LOCUS       DAOSTH010000001       239186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_37_length_239186_cov_11.852077, whole genome shotgun sequence.
ACCESSION   DAOSTH010000001 DAOSTH010000000
VERSION     DAOSTH010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 239186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 239186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239186
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37_length_239186_cov_11.852077"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            293..2800
                     /locus_tag="PLB65_00005"
     CDS             293..2800
                     /locus_tag="PLB65_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ76935.1"
                     /translation="MKEASSQIEDFIKTFRTDKRFNSTIVAENIKEEKQAKTADFPKG
                     LLPYIPSLVKSMGINELYQHQAESIKELLEGKNVVVSCGVASGKSLVYQSVILNQIIQ
                     KPQSRALLLFPTKALAQDQAQKMQSLCNELCKQNPRIPSIINGIYDGDTPSEIRGKLR
                     KQANILFTNPDMLHIGILPNHTLWSAYLSRLVYVVIDEVHIYRGVFGSHMANVIRRLK
                     RITNIYGQKPQFIFTSATLANAQELAETLIEEPVELIDNDGSPQGERHFYICNPPMVE
                     PSLGIRRSSTMEISSIAKSFLRTNLQAILFTGSRRSVELIYRYLVDDKKVADKIRSYR
                     SGYLALERRKVEKELREGKIGLVISTNALELGIDIGGLDAVFLNGYPGTICATRQEAG
                     RAGRKGNPSFCILEAGSNPLDQYICQHPEYIYENNPEQALIDPNNSEILRMQLLCAIS
                     EMAFKEGENFGALSFAEIQGYLYSLLDEGLIKHISDRYIGISNSYPAADVSLRNAGNQ
                     FQILADNELVGWVDSGSVKWMTHPEAIYLHQGETWIVKELDFGQKKVILEPIQVNYYT
                     QAIQYTEIGLDDLLKLENVTGGRKHFGKVTVSRTITGFKKLRFYTLEVLEQEELDLPP
                     EVMQTQAYWISLSGETVEKIRNQGLWANDKNDYGPQWEKLTEKIRQRENYRCRNCGVT
                     GDLEVHHIIPFRRFDDPDEANDPDNLVALCPRCHKLAETRVHIQSGLAALAYLLGNLA
                     PFFVMCAPQDLGIHSEDKSPLALGNPVIAIYDNIPGGIGLSGKLYELHNQLLYAAIDR
                     VQGCSCENGCPSCVGPVAENGLGAKEEAIAILKELIS"
     gene            2900..5428
                     /locus_tag="PLB65_00010"
     CDS             2900..5428
                     /locus_tag="PLB65_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOQ76936.1"
                     /translation="MIDNEGTMKKAMSILVLLLIVTILSALPKELTRSGNPELYKEFS
                     KKARVNLKQLQYKQKEAYQKMLKEHPDVLMCYLIAYECDANLAAADPEDIESNYQTIC
                     ALVNKEGLKYKPEFFLSYIAKQTVSDEAITAYRKAMLKDGLQNIINTTHNDIDRFRAV
                     TLWCVSRLQFQQTSGRDQTPLDIIHKSIIGRCEEMQILLVAAARTVGLPSRPASVPWW
                     AHIDNNHAWTEIFLDGEWHYTGDMDMAYFPDQTWFSGLVDKTVMILAEGSLATWDDEV
                     LAKGKYETLINSTRNYTKERSRTIKLKVINENGKPVTNAYVIPMVFNWGSLRALTVLT
                     TNKNGYLQFTAGRGAFYLSVYSKDGKALILVPSNEKTNVELTAKLDNNDFPGGWATLE
                     YPGNIHNWQTQPEEYTLAVNLEKQLWQEKQKAFETNAIKAKVKDDSLMIKVAIACRGN
                     FTAFWDFVKRNPTPEASFLEFLLLGDPKFLWQAKAEQFEALYENWIRLQDPDIDEENT
                     LSLFSSSVFYEDLPEPIEYVKGKAQLYPKAFRFNYPHNREGAIKVLSKLKQKYKIKPE
                     KALSGLPPLHIAIEHKYLTPVQYRIMAVYILRANGFPAEFSRIPNLVAVFIDGDWQYV
                     NIVKLSWEDRSKDDKASTFHCTLQINDTDNNPIKVEEEQLTLCRYENGMFYPLNVNFD
                     YLGSGKYTGIFPVYETWLQFGYRVSDSKTKFYYQQIKPNIDDVIHLEIIAEEYPRKWE
                     AADSELIALLDDIQNSENKIILIGNYDQENSRRLADKLISLKKDFVWLGYSTSETAPQ
                     NYILSNKWNSMVRENTHNAMRSITLIKIGETWQMYDGLWDKLPD"
     gene            5513..6325
                     /gene="mutM"
                     /locus_tag="PLB65_00015"
     CDS             5513..6325
                     /gene="mutM"
                     /locus_tag="PLB65_00015"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOQ76937.1"
                     /translation="MPELPEVETIVKGLKKVLKGKVINSLECYYPGTIIYDTDIEATP
                     FPAKVLELCRRGKYIIITLETDNAIIIHLRMTGKLVFDVLPSEPLQYERARIILHNSE
                     VLHFIDIRTFGKIIICKQQNEEQYLPILGIEPFSKNFNANHLKAILKGKEASIKTVLL
                     NQSIIAGLGNIYTCEILYRAGINPLMPAGKLSHKEIVEIVQQTLAVLSEAISLGGTSI
                     SDYRNIDDKTGSFQDFLKVYQKQFCPLGHKIERIKQSGRSTFFCPICQKNKS"
     gene            6424..7617
                     /locus_tag="PLB65_00020"
     CDS             6424..7617
                     /locus_tag="PLB65_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HOQ76938.1"
                     /translation="MKALKLKEGIYWVGAIDWDLRNFHGYVTQRGSTYNAYLIIDDKI
                     TLIDNVKSYLYEEMITRIKDIIDPSKIDIIIQNHIEMDHSGGLPELIKLIPKATLYTN
                     ANGVKGLKRHFKQDWNFQEVKNGESISIGKRTLSFLTTPMVHWPDNQFTYCPEEKILF
                     SNDGFGQHIASTEHFAKDLPKGIVLEEAKKYYANIVLPYSKQVQNVLKVAKGLDIHMI
                     CPSHGVIWTEENIPEIISAYINWANNVSDRHRALVIYDTMWKSTQKLAYTISEAFEEE
                     GVSTQLMNLQTNHISDIMTEVMDAKFIAVGSPTLNSSILPTVAAFMYYLKGLSPKNKI
                     GLAFGSYGWGGQSIPILQELLQDPEQCGFEMMEPIKTQYIPDQEELNNIKNNLRQQIK
                     TKLEE"
     gene            7706..8701
                     /locus_tag="PLB65_00025"
     CDS             7706..8701
                     /locus_tag="PLB65_00025"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOQ76939.1"
                     /translation="MNSCNEQDIYSSVFHSALVAIGITDVEGNYVIVNPTWCKLMGWS
                     EEEAKKLNIRDLTPLEDQRTSAESFENLITNKVNSMRKQRRYKRKDGSIFWADLYCST
                     LYNDEGKVMGVLGIFVDIDKQIIGEQVQKELYRNLETLNMELSSTNEDLKRLARYDSL
                     TGLFNRRVMEELLTKESSRAFRTHRGFGVAIADIDDFKKVNDTYGHDCGDIILIEIAN
                     IFQKNIRLTDSVGRWGGEEFLFILTETTAEGAYIAMDRIRKAVAAEIFDYRQNPIEIT
                     LTIGLSFHHGDPSSKEIVNQADQALYRGKKNGKNQLVNYYNDVSASYITEDNPTT"
     gene            9184..9753
                     /locus_tag="PLB65_00030"
     CDS             9184..9753
                     /locus_tag="PLB65_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ76940.1"
                     /translation="MKTNDQSWKPTLTLAKLFEEQNQFYEALAAYEVISQNESSPEFR
                     EKIEELYLRILNDPNSDYDPRVEELFAPEELAYLRIMNHQGFNNLAQARAKLEEGMQG
                     SEIIFSQVMPEDVLSDESEVEVLTSILQEIEQQAQLNIVSEKSDFDEYNVQDLLIAIL
                     AKFDKNQKLSEVKLSDLVTLFLEMQSLKT"
     gene            9935..10483
                     /gene="rsmD"
                     /locus_tag="PLB65_00035"
     CDS             9935..10483
                     /gene="rsmD"
                     /locus_tag="PLB65_00035"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HOQ76941.1"
                     /translation="MRIITGIYKGRNLFLVPGITTRPTTSFNREVIFSVFQSYQGCRV
                     LDLFAGTGSFGFEALSRGAKWVDFVEFAPAAVNTILKNIALLGCSDNCHLWRKKVDVY
                     LKSCKEKYDIIFLDPPYNKNLINPSLKLIYENELLNESGILIVEHSPKENIEVPYSDF
                     INTEKYFKNTNFSWLDSKLYPK"
     gene            11089..13548
                     /gene="sppA"
                     /locus_tag="PLB65_00040"
     CDS             11089..13548
                     /gene="sppA"
                     /locus_tag="PLB65_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOQ76942.1"
                     /translation="MRSFSTIFTVLLLLLLSSVAIAQNVSNNNSVLLSDQDFPIANVD
                     NMFIPVSNPSLIGTGTSSGVGLAYLNDEKKWQDHYWLFVNTDFLSYIYEKDHSDNFHT
                     LALGTELFPAFILPNLYVGTNYRWLEDDFENGIFRSGITYRPHNSTSVAFTWDNPKHN
                     SPYYRLGLAFRPFTFSEAIADYRLELSLDANYVRYENDNDYEIKKPVIGLQTQILDGV
                     KIGATYNLDKETAFVNFSLCAANLEAGSLLHSKDNDNYGIAWAQLTSLNYKPFLGYKD
                     SYWYKMDLTGNVVTYAAPKFKIGPFSLYDTKDKSIETIINEINKAKEDPNVQGILLKN
                     PSFSTSLALQEELIATFLDFKSTGKQVCLYYDNIGNAGYMFAAAIADKIYLNPMGSVD
                     LRGLSISSPYIKNMLSSLGIEVLNFRSHKYKNAGNMFSEERMTDAEKEVYDSILQSLY
                     AQILQRIEVGRGDKLTASPDEIINAGPYFIATEALDNGLVDALIYEDQLNKQMKKDFK
                     FVKKQKELTDYRDYAWSKPKENQIAVIYASGNIVSGKGTPGQKIAQETTVDLIRKARY
                     NNMYKGIILRVDSGGGSAQASDIILRELELAQTENKKPVVVSMGGTAASGGYYISCNA
                     DKIIAEPSTLTGSIGVVGLVFNGTEMFNKIKVNWDTVKKGEHSDIGSFNRPWTDEEKE
                     MMTRSIEDVYETFVKRVDIGRSSLDYNQVNEYAQGRVWTGAQAYEIGLIDGLGGMETA
                     KENMQQLLGKKGKITLVDATTKKEGVKISFNISEMNTFTPLKAYNAVNNDYIKLYELW
                     EDYAGDKALMISPELPEILQF"
     gene            complement(13867..14643)
                     /locus_tag="PLB65_00045"
     CDS             complement(13867..14643)
                     /locus_tag="PLB65_00045"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ76943.1"
                     /translation="MEYEPLKNLLENVISVFPCLRKLLYLQFNIIFLRQRYVFREIRN
                     LFHNTDKIKLYDAGAGFCQYSNFVLSHWPESTAFATDLKTNYLRSFANYAADKYPGRF
                     FYKSADLQIFKPANKYDLALAIDILEHIEDDESAIKNLKEALKPGGYLIISTPSDLDE
                     AAKFTSEHIRAGYHKEDLEEKLQQAGFSIIKSIFTYGTFGSLSWKLMLKLPMQITSKK
                     LYIILPIYYLLIYPVCEILMRIDMKTDNKKGTGILIVAQS"
     gene            14721..15584
                     /locus_tag="PLB65_00050"
     CDS             14721..15584
                     /locus_tag="PLB65_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOQ76944.1"
                     /translation="MIVKEINDIKRINLHLHTNVSDGSLSPKTLVNKAREIGLDLISI
                     TDHDTADAYAQIPENVSPLRILPGVEISSRYEDDDVHILAYGCDLTNKALQEMMEMYL
                     VGRKERAKKMISILADMGIKISLEEVISVAGSRELIVRPHIAQVLVKHGFCQTKNEAF
                     DKYIGNNKPAYVPKPEVSVAEAVSIIHKAEGVAVVAHPVKLTKLSYIDDFIPMGIDGL
                     EVWHPDHYQWEIDNLINIAMKNGLYMTGGSDFHSEQDKHNLFDIIPLPEIVIQSIKEL
                     WKEYQCRALSR"
     gene            15563..16099
                     /locus_tag="PLB65_00055"
     CDS             15563..16099
                     /locus_tag="PLB65_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ76945.1"
                     /translation="MPRTIKVKLIRNEFPATYRYSLWLRIFKLLFAFAVCLYAFYFVV
                     KFVSAETPMFFKVLPLIILFIALDGMFKQLMALNRVTFYEDRVCFGFIAKPRLIIPYE
                     NILYIDLVKQITYSIILKYKEGNREKIFKTSASFPKILEIILNLADLAPNAQSSEFFG
                     KIIKHLRTLAGENDAEQV"
     gene            16083..17249
                     /locus_tag="PLB65_00060"
     CDS             16083..17249
                     /locus_tag="PLB65_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HOQ76946.1"
                     /translation="MQSRFDKIIDRRNSGCYKYDALSMIYGKSDLIPLWVADMDFAVS
                     DAILDALSERLEHPVFGYNFRLNDFSEAIVSWEQRRFNWNIHKDWIISVPGLVPALSL
                     FVLAFTEPGEGVLIQTPVYRPFYDAVIDHNRKLITSALINNNGVYSIDWDDFENKLKQ
                     AKLFILCSPHNPVGRVWTEEELCKIGSLCSKYKVIVFSDEIHADIVYPGYKHIPFASL
                     ADFSNFCLTGVSPSKSFNIAGLATAVVIISNSVIRDKFMVMNTKLHLYLGNSFGIKAL
                     IAAYNDSENWLEELLCYLNGNREYISNFVQNELPGVLLSPIEGTFLAWLDFRNWGLPE
                     PKLQEMIIKKAGLALEPGSEFGDKDEGFMRLNFGCPRSILELALSKIKLLAEDL"
     gene            17249..18076
                     /locus_tag="PLB65_00065"
     CDS             17249..18076
                     /locus_tag="PLB65_00065"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOQ76947.1"
                     /translation="MMEQEIIDLYKNPPQKYTEEHKALFMAFSDALNRGIVRSCEKKN
                     DTWIVNEWVKMGILTGFRMGELTAFPWSEKKPFFDKDTIPEKLFTLKDRIRIVPGGSS
                     ARNGCYIATGVAIMPPAFINIGAYVDSGTLIDSHSLVGSCAQIGKNVHLSAGAIIGGV
                     LEPVGLRPVIIEDDVFVGGNTGIYEGIIVQSKAVIASGTVITASTPIYDSVQGRYLNY
                     DSGNSFTIPYGAVVVPGSRRLKSNPEFQVACPIIIKYRDEKTDKAVELEQTLRELLE"
     gene            18170..19747
                     /locus_tag="PLB65_00070"
     CDS             18170..19747
                     /locus_tag="PLB65_00070"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOQ76948.1"
                     /translation="MNYIFTNGFVLNSQTQNFEEKSVLIHNDRIALIGNLQECKMQAK
                     SSYEIINLKNRLLLPAFLDAHTHFVEYAKGRILVNLNGCHTIDEIRNYLISYRDNLSW
                     KLKWILGGGWDRNCLNNPDELSLKLLDTIFPDIPVALMSKDYHSKLCNSLALKIAGIC
                     KDTANPKGGLIEHNSIGELTGVLYESANELIDPYIVYPESEVIIQAISETVDSIYPLG
                     LVGFNSMESIFSRDLMLKTQEKRKKFRFCWHFYPEDYEKVLQEGIKSYEGNEFYKLGG
                     LKLFGDGSLGSQTAAMFESYPQGEKGILRYTDDELFSLVLSAAENGLSSTIHSIGNRC
                     VKQVIDCFLRLKKTGKHNTLFNRIEHIQAIRNEDIPLLKSSGLFASLQPVHIANDIPL
                     INKYWSDIKEQTYSIKSLISTGIEYAFGSDAPIETINPFLGIYSAIERRQNNNPKNPQ
                     FRPEQSINPFEAIWGYTMGAAKSTKSEHKRGSIETGKLADLIVIEDFRQYPSTFWLTA
                     KSELTMINGDIVYVNEG"
     gene            19932..21326
                     /locus_tag="PLB65_00075"
     CDS             19932..21326
                     /locus_tag="PLB65_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HOQ76949.1"
                     /translation="MNALGFKFPIQERKTALYNLEENWYLPLLAEKRGLSLQNIKRSN
                     FGQYSMAVNYLTSVLEEAAAINKVAVYNIDHMAQLYFYGPDVVTLLNRALTGNFTDMK
                     VGQCKYSLLLNDKGGVQDDMILMRVSETSFILVINAGHDITDTVEIDNKKQEYIADID
                     RIMACKKKGGEEVFAKDISDTLVKIDIQGPLSYKLIKEVYGADVLKNRNNPEKNMSFF
                     TFNEFELNGHHYYLSRTGYTNRWGWELYIPAAAAEEDAKKIITKALDFGGLLVGLGGR
                     DENRISAGPFGLPLMGQEYDPYHTPLNAPLFETAVDMNKDYFVGKDALAKEIAEGNQK
                     RLYIFVSEGIVSNRGIYKEGKRLGTVTSSINSPNVSLEMRLAIGSKRKNVNEENGTAA
                     IGLGWFYAPLFEKDIEGKEIAEIEGKPIRIPVEFYREDENRNPIGSPVLGFVTLEGIT
                     PATAHKALKNIENL"
     gene            complement(21554..22204)
                     /gene="udk"
                     /locus_tag="PLB65_00080"
     CDS             complement(21554..22204)
                     /gene="udk"
                     /locus_tag="PLB65_00080"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HOQ76950.1"
                     /translation="MLPLNNVRLILIGGGTCSGKTTIAKAIGMRLKDLKTVIVAQDNY
                     YRDLSNLPFQQRMKVNFDHPDAIDVDFLLSDLELMLEGKSVDIPDYDFVTHCRKEGKT
                     CVAFADLIILEGIFALYYPELISISDLKIYVDTDSDLRLARRMQRDIVERGRDVENVL
                     DQYLSTVKPSHEAFIEPTKKNADVIIPGDRDFDKVLYMLNGYLLYELIANNREHKH"
     gene            complement(22188..23363)
                     /locus_tag="PLB65_00085"
     CDS             complement(22188..23363)
                     /locus_tag="PLB65_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ76951.1"
                     /translation="MATISKKVLFIDTPFAIEEGLRAKRSRFLWDIVSRNFDADLLLL
                     KSDIYREKPISSHSGYDKLYSLSLKSENKLEPIIYHHLGKGQAGRFAHILDSKRYEII
                     FFGGLACLPLLKIANRVLPNCEMIIDVEKVYISDLKEKWESNRELNNISYLWEYIQQY
                     IWDKYLINKKPNCLYANPGDLDLLCNFYKINKSNSHFVPLPITDLRFSESTEKDKKYI
                     IFWGSPENKDNLTVAKNIVSEIYPRISKKLVEKNIGIILCGEEKMQEFCSGRISYAPY
                     DDLDNILEQALFIILPLEKPDTEGRILSCAAHHKALVCTKIAIQNFPVEENCYLQGDC
                     FTEVSDKILELLRGINKLNEYSDNFFNACWSNFQAAEIERNLLERINTWIKKDVTTQ"
     gene            23716..23988
                     /locus_tag="PLB65_00090"
     CDS             23716..23988
                     /locus_tag="PLB65_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOQ76952.1"
                     /translation="MKLRPVEDNVVVKLNTADNEKTIGGIIIPDTAKEKPQIAEVIAV
                     GNDEDLQKIVKVGDKVLFGKYAGTEIEFEGEKLLILSKSDLLAIVE"
     gene            23994..24317
                     /gene="trxA"
                     /locus_tag="PLB65_00095"
     CDS             23994..24317
                     /gene="trxA"
                     /locus_tag="PLB65_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOQ76953.1"
                     /translation="MSIVAVNSSTFEEEVLKSSLPVLVDFWAPWCGPCKALSPTVDKV
                     ANETAGKVKVVKVNVDEAQDIAAKYSIMSIPTLLVFVNGVVSDQLIGLVQKDKIMEKL
                     NKFMQ"
     gene            complement(24605..26062)
                     /locus_tag="PLB65_00100"
     CDS             complement(24605..26062)
                     /locus_tag="PLB65_00100"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOQ76954.1"
                     /translation="MKMWRQLVLVLIALLMTSCASAKDNMIDPNGPNPVVEVVKNVRE
                     AVVQIRVEAKVTVQNYINPFLDDPWFRQFFRFPQEQTRPIVSLGSGFIYEYNPGSREA
                     FILTNNHVVEKGRDGTITVTMADKVVYNASIVGLDPSTDVAVIKIVVKEGEKVTVAPL
                     GDSDKLQIGEWAIAIGNPFSEGLDRTVTLGVISATSRSNLNLGDNSPIYQDFIQTDAA
                     INSGNSGGPLLNIKGEVIGINSALASTNGGNVGIGFAIPINLAKRVVEDLVASGKVTR
                     AYIGILPQEITPDIMEAFGLKEVAGVLVAKVEKDSPADKAGIKTGDIIIEINGEKVPS
                     VPKFRIAIATARVGQQIPLKIVRDKKDMTIRVTLEAYPEDIAAASDSVKGGIATGISV
                     EAIDSQTAKRLGVTSDKGVVVTKVDPNSPAAKSGLKVGYVILQIEDKEVNSPKEFNTE
                     LEKAKTNMEKENRKTIRLYVLDTNKQPRFIVLKFE"
     gene            complement(26303..27724)
                     /gene="thyX"
                     /locus_tag="PLB65_00105"
     CDS             complement(26303..27724)
                     /gene="thyX"
                     /locus_tag="PLB65_00105"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HOQ76955.1"
                     /translation="MKVNVAGYNIDKGLIDKLNSPVATPELISAAYARISRSNKSIED
                     LRKEAITELEKARKTNTNIIFEMGHSSVAEHAVFNIDILGISRALTELVQRSRIASYT
                     EKSQRYVTFGKDYIVPKEFANRPKLKQPYKQMMDTLFEEYEYCFNALNELYKETKPEL
                     KNRQREYLAKEDARYILPLSTKTQMGMTINSRSLENLLRRLSKSPLKEAAELKDLLFK
                     SVYAIAPSLIRYTEDDNFSGIVNLEKVGFTDFLQQELPWVKELDMENKLNIIHTPNNI
                     DDTVLAAIIYQQGELSWNETKETVAHLPKIIKRQLWNQVFTNLKPWHKVPRAFELIDF
                     SFEITMSESCWAQFKRHRYCTIIQKGGSALATKIPPSIKKINRDKIWSDLNTQLIRLG
                     SSLPPKLAYLSPYFRTNASLVTILAKMNLREIYHFVRLRSDENAQWEIRELSQLMAKQ
                     VKNAAPQAGFWLCGKSEFPKQSS"
     gene            complement(27737..28513)
                     /gene="deoC"
                     /locus_tag="PLB65_00110"
     CDS             complement(27737..28513)
                     /gene="deoC"
                     /locus_tag="PLB65_00110"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOQ76956.1"
                     /translation="MKDIPQIAKDLFGDNHLAQCGGGHKICLNCYNCHLKSPERICDP
                     DTNLASVIDATILKAEAKRKDVEELCKTANLYKTASVCVNSYFIPLVKELLEQPVKIC
                     TVINFPLGASSPEAILNEAIAVLEAKAEEIDMVQNLSALLSGDYKNNLLSTQEVVNIC
                     NKHNALLKVILETCYLTEEQIIISALLAKKAGADFIKTSTGFGSQGATIENIALMREV
                     VGPKIGVKAAGGIRTKEQALAMIKAGANRIGASNVVAILQ"
     gene            complement(28510..29562)
                     /gene="rlmN"
                     /locus_tag="PLB65_00115"
     CDS             complement(28510..29562)
                     /gene="rlmN"
                     /locus_tag="PLB65_00115"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOQ76957.1"
                     /translation="MFTNIYSLLPEELANKIKIEQLGSPVYRTKQLLFWLYKSYINEP
                     DKMSNLPAEFKTYLLNNYSFYLPETDSKLVSSDKAIKYRLKLEDNELIESVLIPTEKK
                     NTLCISTQVGCGRNCQFCSTAKMGLIRNLYNYEIVGQVIYAARQLKSSDNKKLTNLVL
                     MGMGEPLDNLDNVLSALRILQSQEGLSFSPRRITVSTSGVVPGIIALADSGIKAKLAL
                     SLTSAIDSKRSKLMPVNKQYNLIELKQALLYYLRKSSYRITIEYILIPSFNMDDDDLK
                     ALSKYVGDLSCKINFIPYNPGKDSIFRAPTVKEINDFMYRAQSLPQAITLRKSRGADI
                     FGACGQLNKNNLGAKI"
     gene            complement(29562..30395)
                     /gene="ricT"
                     /locus_tag="PLB65_00120"
     CDS             complement(29562..30395)
                     /gene="ricT"
                     /locus_tag="PLB65_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HOQ76958.1"
                     /translation="MQQYIEVEFRTGRIGYYRNREELNIQPEDLIIVEVERGDDIAQV
                     VHLGLEEDEIAPEVLTSKNFSIRRHASSEDIDKMRNLSYEEEKASRTFLSILERYPFE
                     MKLIETIYQFDGNKLTFFFTAEGRIDFRTFVRELATVFKTRIELHQTSGRDEARRLGG
                     FGMCGKQYCCGSFLKRFNQVTIKMVKDQNLAGNLTKISGPCGRLLCCLNFEEDFYVEE
                     VKGFPLIGTFVSYQNTKMMVFRLNVIGKKIMLASEEGIIQEIDLDEFSKLPIISLPIL
                     E"
     gene            complement(30395..31588)
                     /locus_tag="PLB65_00125"
     CDS             complement(30395..31588)
                     /locus_tag="PLB65_00125"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HOQ76959.1"
                     /translation="MQTIIFDDASRSNFFPLTLNRSIGDLRCGITKLRQRLEHIFSDT
                     DTTCVIIDDSLVPLYKEHHPTWIINSLVNGEKLYLNSRIIVTVEAIAQIKDLPMGYCL
                     YSGNDLVAIKVNDSSVWPDLLKLKKKLGNIRLYDSLADLIQDNSRSLVRDYHLHFYDG
                     DNYFETEPGVTVLHPYNIWVAEGAEFSPGVVLDASEGPIIIDTSVRIMANAVICGPVY
                     IGKKSVIKIGAKIYGGTSIGPVCKVGGELEGTIFQAYSNKQHDGFLGHSYIGEWVNLG
                     ADTNNSDLKNTYKNVAFYSYPLKTKIDSSTQFLGCMIGDHSKTGIGCSINTGTVIGIG
                     CNLYGPNLIRDFVPDFSWGQADELDKHRFSAFCETAAIVKQRRKLIFTDTERELYHQI
                     YNMRL"
     gene            complement(31569..32738)
                     /locus_tag="PLB65_00130"
     CDS             complement(31569..32738)
                     /locus_tag="PLB65_00130"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ76960.1"
                     /translation="MSSVIFYILSALILTGCITYFVFCIRFWNGYRTYKPTFNYKKRT
                     VSVVIAARNEAKNLQELLLCLLNQDYPKELFEVILADDDSEDDTEQVAKKYIDAGLNL
                     HYLKVLGRENVISPKKKAMEDAVYATQGEIILTTDADCLVPETWISSMVSMFTDDVSM
                     VAGYSRTYIPDWKKASLVQKYEHLDFALTYMVLAGGYTIGKKWACIGQNLAYRRTAFD
                     DVGGFTKIKHLLSGDDVNLLQLMRNKGHKIIFNFLPSSFVYTHPVTSWKQLINQRSRW
                     ASNMKYQLKFNPEFFFILLSMAFMYWGGLIMLFFNIKIALIIFLYRIIVENITISYSR
                     SRFGFTKEMLRFYPIWLVIQNFFLVLTMVLGQFNIFVWKGKRPTSKRNNNANHNI"
     gene            complement(32742..34802)
                     /locus_tag="PLB65_00135"
     CDS             complement(32742..34802)
                     /locus_tag="PLB65_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HOQ76961.1"
                     /translation="MNAHTQEKAHKILKYLWFAFCIFTGIFVGALWFYRDSLPPTSEL
                     RNYTLRSGSEVYDRNGRMVYLFAFEKRKLVSLKELPPYLIDALIITEDKHFYHHFGID
                     LIGNFRALVIDIARMDFSQGASTITQQMARNMFLTLDKRVSRKMKEIILALRIEREFT
                     KDEILEIYFNKIFWGGQVHGVETASLYYFGKHARDLNLAESAALIGMIQRPNYYSPVK
                     HPERLIERRNMILGKMLKAKKITQEEYNEAVASKISSDRSSMQQYSTDYYIEHIRLYL
                     ERKYGTEKLFEGGLRIYTPLDAELSVYADSVLNRYLSKIEQSYPPAMRYSNVSKNSVD
                     INTRYLQGGLLLMDNNTGHVLAMIGGRNFTHSKYNRITQAKRQPGSSIKPTYYTAALE
                     KGYTPATVIIDSPITLTGGDGKEWTPQNFSRQYYGPTRMRTAITYSYNVWAVKAVMDI
                     GLDVVNDAFLRFGINTKAADYSAALGAYEVTPLNLISAFTTFPNGGYRTKPIFITRVE
                     DMNGKVLERSATSKYQVTSPEVAFLMTSMLQSVVTSGTGAAARNNYYWQAAGKTGTSS
                     DHRDAWFIGFNKKYTLGIWNGFDNNAAISASASCAPIWGQIMTKAIRIDNKGRTPRND
                     DPRYSFVVPDRIVKRTINPKTGFLSDTGIEEYFIEDNIPSARSDTLSYNFYPTRWGTK
                     SKLD"
     gene            complement(34799..35323)
                     /locus_tag="PLB65_00140"
     CDS             complement(34799..35323)
                     /locus_tag="PLB65_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HOQ76962.1"
                     /translation="MNIDNKSTLIQLAFFTAIAATIHIVEDLIMRMLPLPFIRVGFSN
                     IVILYLIYHNKILEAFVVNISKTIISGIATLTLLSPSILISLTAGMAAIIVMVFVRWL
                     HLGLGIYGISICGAIAHNLMQLTIVYAILIHSSRVFVLTPILLSIALVSGSIIAYLTL
                     YIDRKNEGSEIIKE"
     gene            complement(35320..36036)
                     /locus_tag="PLB65_00145"
     CDS             complement(35320..36036)
                     /locus_tag="PLB65_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ76963.1"
                     /translation="MIEVNKLYVSLNEREILSNINFKLEDSNNLVILGRSGSGKTVLI
                     KTLLGIYYPASGNVIIDGIDIHRADEEQQKATKKRFAMVFQNSALLDSFTVLQNVALP
                     LYERGEKNIAYVRDKVLHCLQIVGLEDTISLYPAQLSGGMRKRISIARALVYDPDYII
                     FDEPVSGLDPITSREIMYYITRIVETASATLITITHELKNIDAIGNQILFLNEGKVIF
                     SGSVKQLYNSTDSFVQQYLK"
     gene            complement(36023..36379)
                     /locus_tag="PLB65_00150"
     CDS             complement(36023..36379)
                     /locus_tag="PLB65_00150"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOQ76964.1"
                     /translation="MKFFRTIYPTLFWLIIIWILSSIPSQDLPSVNIVGFDKLQHLGV
                     YAVLGCLIGYWLRFKNWDIKKIVLIYLGLIVLAGIDEYHQRYIPGRDVSIYDFLANAS
                     GLIIGFLLYLRKYDRS"
     gene            complement(36376..37056)
                     /locus_tag="PLB65_00155"
     CDS             complement(36376..37056)
                     /locus_tag="PLB65_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOQ76965.1"
                     /translation="MLVNGFLAAITTDYYMKAVGIKIAQISIQQNKADNYVEIRAKSV
                     RESSIFPQINNVYRVKHNGSYQPLTYTRIIHQKNVQDEVTTTYNHNTAQAVMYRNSDK
                     STLQYSITKNSQDIYSFLAKVISGQVDTGTYPIDANGVSWQANITRYSTEIVDTPLGK
                     YPAVRYEISFQNMTNKKMPYIDMVTFNMLQENNKLNLWVYNRQFAVKATFKKKGISST
                     WELCNIKK"
     gene            complement(37077..38342)
                     /locus_tag="PLB65_00160"
     CDS             complement(37077..38342)
                     /locus_tag="PLB65_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOQ76966.1"
                     /translation="MDDGKAFIIIVLFALSAFFSGSETALFSLSRVYLKRLENSKSKS
                     AKRILKLLKKPRELLITLLLGNTFVNLAISSFGTLLALQIGRKYSYTESSVVTIQIIL
                     TTILILFFGEISPKLIALSKADTLSGILSLPLLMFRYLFYPIVWVLVKMSELISSHQN
                     VDRYVGTKFTDEEFHNLIQSESSSHSLEEHEKKMLVGLFRFREAEIKEIYVPRVKITA
                     IEENQSIEDLKKIIIESGYSRIPVYRGSIDEIVGIIYVKDLILYPEKKTIKQLMRPVW
                     FVTENMKVQTLLNQFKQRKLQVAIVVDEYGGTSGIISLEDILEEIVGEIRDEYDKEEI
                     PELIKIDENTYILSGNFSIRQFNENFSTDISLEDYDNLAEFLLAYYNHVPNIGDTVFY
                     DERIEFTILDADEKSIKQIQVKIKTGELT"
     gene            complement(38345..38809)
                     /gene="ybeY"
                     /locus_tag="PLB65_00165"
     CDS             complement(38345..38809)
                     /gene="ybeY"
                     /locus_tag="PLB65_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOQ76967.1"
                     /translation="MNSLEVLGAVIIPESEQEKIAELICSKEDPDHCYQICVKIVDSN
                     TMKRYNKRYRGLAETTDILSFVTAELPFCESDLTFTEDGFPDKPIRFCDIIIDINQLF
                     YQRDSNIIEDNYRTVLIHGLLHLIGYDHIRSADAEKMRAKEEYYFKLTQGEN"
     gene            complement(38802..40898)
                     /locus_tag="PLB65_00170"
     CDS             complement(38802..40898)
                     /locus_tag="PLB65_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HOQ76968.1"
                     /translation="MNSKYILLCIVTAIVIVGIYQIVAVGRVNYPEYNLKEGQVADVE
                     IIAPFDYPILKSTSELADEREQVLSQVNKPYEISDDPMFEAIKALDNIYAVLCSLQPT
                     LDPNAIATELKRNGYIFSSEGLSFATHPMLRDKIYESLRNKLRAIYKQGIYETLKEDS
                     VLIDQNGKLKTVNIREFLSIEQVKEDLVTNFPEAKTFVLEFSEQFLKPNLQVDEEKLN
                     ELNQDSLGTIYQSKGMVLRNEVIVRKNSRITQDEVRKLESLQEAYRRQKIRKSPLQEM
                     FLTLGLLMFVFLNIFLTNHYFGVQSKEEKIPVADFIPVNLGFILLVVFTVLSNYALSA
                     NNLIIPFAMIVIAGAILVNFEFGLLYSICNILVISPFLNWETFTPVIMILSSVICLLI
                     LRRQNAWHEYLMIWLYLLLSQIVVIISIGIYKRDTLLTILRSTGYSFISSTLSVSGIL
                     LIVPYYEHKWNRATKQTLLELLDFNHPLLKRLATEAVGTYHHSLVVGSLAEHSAEAIG
                     ANPLLARVGSYYHDIGKITNPDIFTENNEDSADIHSQMEPEESTQLIRNHVTDGIALA
                     KKYKIPQPVIDIIMQHHGNSVIRYFYEKASKEKKELSKDTYRYPGPRPQTKEAALVML
                     ADIVESTTKAKNIENEAEVAKIIDDTIHYLLKEGQFDEAPISMKDLQTVKQCFIPVLT
                     SIYRKRLDYPEPPKDE"
     gene            complement(40986..42131)
                     /locus_tag="PLB65_00175"
     CDS             complement(40986..42131)
                     /locus_tag="PLB65_00175"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOQ76969.1"
                     /translation="MKYRKMPYLDDKLSTLGFGCMRLPKNKEGKIDEEQALAMLHYAY
                     ENGVNYYDTAWSYHNGESEPLLGKFLSQIERNKVFIATKLPCWLVKNRADMDNFLEKQ
                     MEHLQTSYIDYYLLHSLNQKSWMEMKRLGVIDFLDKAKMEGKVRHLGFSFHDSYPAFK
                     KILLAYQWDFCQFLLNYLDTNYQAGMNGYKLAGQNGTGIVAMEPLRGGKLAGHLPENV
                     AAVWKKHNIRLSPVKFALRWVWNLEGCQVLLSGMSSLEQVKENVHYANLCKANVLDAK
                     TLKIYKEVRQEFLKRIPVMCSECRYCLPCPQKVAIPDIFGIYNDSIMFNEKDRHQKEY
                     ELFIPDESKADKCIACGACVSKCPRHINIPEEVKKIAEYFAECKVID"
     gene            complement(42128..43942)
                     /gene="uvrC"
                     /locus_tag="PLB65_00180"
     CDS             complement(42128..43942)
                     /gene="uvrC"
                     /locus_tag="PLB65_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOQ76970.1"
                     /translation="MQALSKEIESKLSLLPDQPGVYLWKDKNGTIIYIGKALNLKHRV
                     RSYFASSGKDVKTEHLVKNIADLEYIITNSESDAFILEATLIKHHRPKYNIMLKDDKR
                     YPFVKITVNEPFPRIFITRELLKDDAKYFGPYTEVRYLRSTLRDLEWIFPLRNCNRNI
                     PKTKYKQPCMNYQLGKCPAPCVGCINQKDYAVIVNRLLRFFSGRYTELIDELRAEMQN
                     ASDSLDFEEAAKLRDRIVAIQSIQKRQSVFNVEGGNTDIIGFYREEKRAVCVVFKMQN
                     GAIIHQENYPLANLDYEESDSILASFLQLYYADKEEMPDEVLLPFAPKDIDKLNSWLG
                     NKLQVPQRGEKTKLIAMAKRNAFNIVEESKLAHLRKANRTIFPIQELKEVLKLPKLPR
                     KIVCMDISTIQGSDTVSSAVFFENGKSKKKYYRHFIIRSIDTQNDFAAMQETMARFLT
                     ETEKDEQMKPDLIIVDGGKGQLSASEQILNSSGKNDISLIALAKRAEEVYLSGNEQSI
                     VLPRSSSALRLLITIRDEAHRFAITFHRKRRTQRTLESALEAIPGIAETNKFLLLKEL
                     GSIDNIAKADIETLSSIKGIGIKTATKIYNHFHNEENK"
     gene            complement(43918..45492)
                     /gene="murJ"
                     /locus_tag="PLB65_00185"
     CDS             complement(43918..45492)
                     /gene="murJ"
                     /locus_tag="PLB65_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOQ76971.1"
                     /translation="MESQHSNRTLAKNISVMSIGVFFSRILGLIRDQVMAFFFGTTYL
                     NDAFNVGYNIPNLLRRLFGEGALSTAFVPIYNEILIEDGKDKQIDFALNLLSILTLIL
                     VILTILGIAFASLIVKCLYPGLAPETTVLAIKLTRIIFPYLFFIGLSSTFIAILNSHN
                     YFFMTGLSSALLNVGMISTIIIPYYVLKVSGEELIIWAGWGVIVGGFLQTIVNFPYLK
                     KIGYRWAFCFRFGSTALAQLWKRIIPSMIGIGIREINLIADSLMASFLPIGSITALSF
                     GNRLMQLPLGIFGISTGTAVLPFYSRCVTNGKYDELSESIRFTGLNLAYIMLPVTTII
                     LALGEDFVRILFQSGAFNERAVWMTSQALIFYSLGLVFFGLNQTITPIFYAHKDTKTP
                     VKIAAAMVALNITLNFILMQFMAHRGLALSTSITAGVNYFILLYLIRKKMPGITFSGI
                     IRNIAKTLLICLIIFFLLVIGRNILPVQGRIELLIRDAVFSVVAFGLFYLSAILIKID
                     YITQAGKALCKRFLRK"
     gene            complement(45493..46605)
                     /locus_tag="PLB65_00190"
     CDS             complement(45493..46605)
                     /locus_tag="PLB65_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HOQ76972.1"
                     /translation="MINKIKGQEDALKLLRKAIENERISQAYLFYGSDGVGKFTTALY
                     FGMAVNCMATNELKPCGHCDSCHKFLQLEHPDFVYIFPTPNLNLSPEGEIKNSDALKM
                     FQKYIENKVNTPWADFFFKDTIEIRKESITMLSKHFELSIHEARYRIVIVEDADMMTI
                     ATANAFLKTLEEPPERTIIILITERIQKLLPTILSRTQPIYFRPLTRSVIEEILTTEF
                     EISAPIARTASRISAGNLKTAIRVASDSVSLSRNWAFEIFALAATKDDLGYLGLAKAY
                     KDQQKKDQVIDLLKYIRIIASDLGALNANFEAEITNIDKIDDLKAMVENKSGIDDRLY
                     DFLLFLEDLNRKIEGNVNLNLVMTNLYLNCKHLLNS"
     gene            complement(46606..47211)
                     /locus_tag="PLB65_00195"
     CDS             complement(46606..47211)
                     /locus_tag="PLB65_00195"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HOQ76973.1"
                     /translation="MKYFRKLVGEKCYLSPVSMDDVEKYTEWLNDMEIGQFVTLSHQV
                     IDIEKESELLRELMKNNCIFAIVEKDTNKVIGNCGIHQVSDVHRNASLGIFIGEKTFW
                     NQGIGSEATNLLLDFAFNMLNMHNVYLSVLAYNKRAIRCYEKIGFKKVGVQREFMFVS
                     GQYHDVIIYDMLASEFTSPYIKKVYTYSVSDEAGRSKISIV"
     gene            48185..48270
                     /locus_tag="PLB65_00200"
     tRNA            48185..48270
                     /locus_tag="PLB65_00200"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:48219..48221,aa:Leu,seq:tag)
     gene            48302..49594
                     /locus_tag="PLB65_00205"
     CDS             48302..49594
                     /locus_tag="PLB65_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOQ76974.1"
                     /translation="MQVEFNQINVVIKEINITIPSDEVTKAYEKYLVKIAKEIEVPGF
                     RKGKAPLYMVERVNTEKIKENFCYDYVIDVFDKVAEENHIEYLLYPDIKDLQWEKGND
                     MTVKIQIEHEPQLELKQIEGLTVPYKPAILEESVEKFIADLIAQNTIIKDVEIAEEND
                     EVEVELIVQNNSDTYTVSAIFYACSEDNDYSIPALIGHKIGDTLSVILTGKKIQTNAL
                     NADFNLVDDTEYSCELCINRISRKEIPALDDEFAKDMEYDSLEEMRAKIAEEMKLRIE
                     HINIDIENNSIINKLFTDNPFPLPMKTVEYLAFQQISDVSDDNLRKLLLNRYYYMIMN
                     QMVQLYIGNALMKEKPIELTDEMVEEYINHQAIIQDVTVEAYKEHSKIDFSSQDTQNS
                     IKEYFILRQIAKTCEFVIEKEPPETENTESGTEKIEEE"
     gene            49594..50178
                     /gene="clpP"
                     /locus_tag="PLB65_00210"
     CDS             49594..50178
                     /gene="clpP"
                     /locus_tag="PLB65_00210"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOQ76975.1"
                     /translation="MSFVPIVIEQVGRSERAYDIYSRLLKDRIVFVGGVIEDNLANTV
                     VAQLLHLEGEDPERDIYMYINSPGGVISAGLAIYDTMQYIKPRVSTICIGQAASMAAV
                     LLAAGAPGKRTALPNSRIMIHQPMGGAQGQATDIEIQAKEILYLRGRMNEILHKHSGQ
                     TIDKIREDTERNYFMSAEEAQEYGIIDEVISMRK"
     gene            50268..51515
                     /gene="clpX"
                     /locus_tag="PLB65_00215"
     CDS             50268..51515
                     /gene="clpX"
                     /locus_tag="PLB65_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOQ76976.1"
                     /translation="MEKYRTLTCSFCGRAEHEVKNLIRGIAGNICDECIVMCHTIIDS
                     SKEEISQEEFDAPVPSRIKAYLDQYVIGQDNAKEIISVAVYNHYKRIFKQKYKDDIDL
                     EKSNILMIGPTGSGKTLIAQTLARFLQVPFAISDATTLTEAGYVGEDVENILVRLLQN
                     ANYDIAKAERGIVYIDEIDKISRKSETPSITRDVSGEGVQQALLKILEGTKVNVPPKG
                     GRKHPQQDFIEMDTKNILFIAGGAFFGLEKIIKERMDKKSVGFDVELGKRQDEATIFD
                     YTTPQDLLKYGLIPELIGRMPVICTLHELSEEALVDILVKPKNAICKQYQKFFEMDGV
                     QLEFTEPALKEIARTAIKQKTGARGLRSIMEKFMLKIMFSIPDRTDIEKCIITPEVIT
                     NSQEPRYEYIKPLNSRNKVANEK"
     gene            51530..52768
                     /locus_tag="PLB65_00220"
     CDS             51530..52768
                     /locus_tag="PLB65_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HOQ76977.1"
                     /translation="MTSEVTIPRREKLLSTLKEKEALIIFAAEEAKLEKFKQDNNFLY
                     LTGLDIPEAIYFAFRGQNGAIEMLFIQRGDPEKEIWTGAKMTAEEASQKCGLSLQKIH
                     YLDEFLSILSFYCPVIDKIWANIGEQYLDKPPTMQLFRLSPIRERYPNIIIESIETII
                     TPLRKIKSDWEIKQLQRAIDITGKAIIDVLEKAEALMMEYELEAIMFYRMQSMGIKHW
                     GFAPIIGTGINAATLHYEKNECQIENGDVILMDVGASYLNYSADITRCFPINGSFTDR
                     QKEVYEVVLDVQKRVIEMIKPGIELLELNQVARDLLAKGAIELGLIDREEDIYKYYMH
                     SISHFLGMDTHDVGSRNAILETGNVITVEPGIYIPEEKLGIRIEDDVLVTDTGYCVLS
                     QHIPKEISEIEEIRIKAMTK"
     gene            52765..53247
                     /gene="coaD"
                     /locus_tag="PLB65_00225"
     CDS             52765..53247
                     /gene="coaD"
                     /locus_tag="PLB65_00225"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOQ76978.1"
                     /translation="MMSKAIYPGTFDPITLGHMNILEKACSIFDEVILAVADYTGKDN
                     LFSLQERYQLCKKSTEHIPNVSVSTFQGLFVDYAKSQNCNLMIRGMRAVSDFEYELSL
                     ALTNKKLNPEIETLFLVPSLKYMYLSSSIIRQLAALQGPLEDYVPTCVAEAMREKHFS
                     "
     gene            53252..54628
                     /gene="dnaB"
                     /locus_tag="PLB65_00230"
     CDS             53252..54628
                     /gene="dnaB"
                     /locus_tag="PLB65_00230"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOQ76979.1"
                     /translation="MAKQVDAEKLNKVQTERALPADINAEAALLSAMLIDSDVVSKGI
                     EKIKEEYFYRNAHKMIFRTMEELFNEGIEIDQLTLINRLERNNFLEKVGGIPYINEIA
                     DFVVSAANFDYHLNIVTEQALLRHLIVASKGIIENCYTAAKPVKTIVDEAEQAIFAIA
                     ELPSHQGFVSFDQVSQEVITNIDKIASTKTSVTGLSSGFGDLDRFTGGFRPGQFIIIA
                     ARPAMGKTSLALNIAAHIAVNLNKKVAIFSMEMAADEVVMRMFSSASEVKMDGMLRGY
                     GMNEEKLIRIMQASEVLSSKQIFIDDSGTNSPLDIRAKTRRLSAEIGGLDLIVIDYLQ
                     LMSLVREKDNRQQEISEISRALKILAKDMKIPVIALSQLNRAAETRDNKRPKLADLRE
                     SGAIEQDADIVMFIYRDEYYFAETTTKPGIAEINIAKNRHGPTGSVELGFDQEFTLFR
                     PLETKYDQ"
     gene            54618..55367
                     /locus_tag="PLB65_00235"
     CDS             54618..55367
                     /locus_tag="PLB65_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ76980.1"
                     /translation="MISNIFAGIGEYTIFVGKCLSKITSLFKRYQEFLIQFKRIGFDS
                     LFLIFLTAAFTGLVTALQAVYQSKGYVPVNLLSVLIGKSTMIELAPVLTALVLTGKVG
                     ASIAAEIGTMRVSEQIDALESMNIDPYEFLYMPRIVAGILAFPLVTVFANAVGIVCAW
                     YFSWLHYGLHYYTFFNNMRSYFVTSDLWGGIAKSIVFGFIITSFSCYHGDRCFGGAEG
                     VGKATTLTVVYSSVFILVMDFIVAWVLFGAY"
     gene            55369..56481
                     /locus_tag="PLB65_00240"
     CDS             55369..56481
                     /locus_tag="PLB65_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOQ76981.1"
                     /translation="MKSWKLSLLLALLLLFSCGQEKNKQGNTHYKHKLVVYTTDEFRK
                     SGFEHSIVPDFQNKHKCQLEFVLFRNTAELCKAIKDKANYGKYDLAIGIDNSFAVSET
                     LAVNFVPPESFDSELLIRETIFDPALRLIPYAYSNLSLIYNTTQIKNPPQSFGELQDA
                     KYLAQIAICDPISSGLGRSTLFWSVALFGSEGYEYLWKSLRKNIYKTYATHTEALEAL
                     KRGECSLMIGYNTTPAFLQELDPLNKDFQVSMLKEGSYQYIESIGIHRGTSKSALCNK
                     FVDYFLASEEQKMVIYKLGMFPANRKTLLPMHFSAIPFISYSVNDRISNNLIQEQILS
                     WLDFWQRLFGYQIALQEGQISWKLESYTPSNINKES"
     gene            56636..57091
                     /locus_tag="PLB65_00245"
     CDS             56636..57091
                     /locus_tag="PLB65_00245"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOQ76982.1"
                     /translation="MFHIVLFEPEIPQNTGNIGRLCVGTESTLHLIQPMRFILTDKAL
                     KRAGLDYWPNLNLQIHNSIEEIYAIFPQERIFLASTKVKKCYWDIKYQKDDVFVFGPE
                     SRGLPIELLNKYPEQGIRIPMSNKIRSINLANSVAIIMYEAVRQINCFF"
     gene            complement(57088..59118)
                     /locus_tag="PLB65_00250"
     CDS             complement(57088..59118)
                     /locus_tag="PLB65_00250"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOQ76983.1"
                     /translation="MKANFQDVQSTILACKTPYQTLIQKLKSTNIMPNMPYEEFFVEV
                     KELCLLSEKYGSILENDPKLETTPFEQLSALHNEYYASLANYENCLGNPDYAVALYNE
                     DMGKFLSALYFHFANMIYLQANRNYLQLEEKLRLFFSLYDKARQGNLNYTDWKNEYQK
                     IVTENHKLKLYRTFYQSYIQEANINYDIIMNTNLKDLRYLYRYGMFISEVNIKMAQFI
                     NAYPEKELNDIACYIVQCWIDGFTRSNKNYRIKQTASIIVPCGMERLGCLIYNELDKI
                     GIKGQFNVPYKIYENKQFTYDHRFDNALIIDRDYTDMKINVYAEVVEDLKDLVSGDAG
                     PVYVELFGETPFSPVAKNTALALTDEQQELSREFMSKSAQIYYQYYRRDQGSFCIIAF
                     PSPEIGEHFPEIFADTLKLNLLDSMHYAKIQQNIIDVLDKADYVNVKGKPGNDTDINV
                     KLHPLQNPDKETNFENCVADVNIPVGEVFTSPLLTGTNGTLHIDDIYLGSLRFYNLKI
                     QFEDGWIKGYSCTNFDDPEEGKKYIYENLLLPHKTLPIGEFAIGTNTLAYQMAKKYDI
                     LPLLPILIIEKMGPHFAIGDTCFSYEEDSPHPSFVNSKEMIAVDNEKSITRKEDPLNA
                     YTHKHIDITLPYEMLESITAVTKDGERYDIIRNGRFVVPGTEELNIPLEEGV"
     gene            59439..62381
                     /locus_tag="PLB65_00255"
     CDS             59439..62381
                     /locus_tag="PLB65_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ76984.1"
                     /translation="MRKPLLLIMLLLIFSTIYAEPVWTNDVAIREVNYIDWQRNAVKN
                     ANNEVFLTWSQSEDGINNLYVQKTDFSGSPQWQEPLVIKAGCVQFNQVKQLVTSDGNI
                     IITWLEEDNSVSSLFVQKYTPAGDLLWGPQGINLSSLRVIDSFEYRIAVNNIGGFLLL
                     LRNDSLSQINLQSYDASCLPVWDNSDLNINLDHPELDDLVADGLGGAIIKFRRFSSVN
                     GINYLRRFANDGALIWATSYTLSNGEIRYPHQLFLNPNGILYDLGFAYRNDSIINIRT
                     YNALAEQINQPLATYQLVLAGETNIENCNLKAVTTNGGDLQIGLLRNTINNDCEIFTY
                     RFNQNLQPRYPVNGLVLSNALYAIDNFDLAVNNDEVAFIWAEYNEATGSILKMERTNI
                     QGSQYGYIYGYNLDQLSGVYDSPQLLYQNNQMLVISKKTIDEYLKLSTDFYTITTKEG
                     KDPQRTDLVSLINGSATLLANIPLADRAMLFYSDSRGGEYHLYCQGISLTGQLLFPEN
                     GKLINLGQLDYNSYKIFKINSEQIGLLHYSNALYLQIISENGDFLLNGNGILISSAQL
                     TRYNATCYEDDIYISWVEPSSIYSNSCIYGQRIHNGTKMWGETGIMLKDLISSPYGYA
                     FTGRYLLWKETVAGNNTILCLKYDENGYPDADWNASGEIVINNLPTNQYSIVASYITG
                     EDLLLLIAEYVIHPVFFQKVTANHTLPWGDEGITFPATFFQLNYKYKADKLYLLYTAR
                     DSGSSSYNVFFQVIDSGGNFLYPEMGLQVNTLAPGNDVSWSDSYANYPTLCVFDNNSC
                     LTVWGSRMSRTDGQDLYYRRINADGNFVESEDQLLCNAPYDQIYPLISTIGNQAIIGW
                     QDRRMSHYTNCYGIYAQRIDAYGSSLPPEITPEVVLFKSCYPNPFSKSTNIVWEQKNH
                     SRVSINIYNLKGQLVKSLTPSSMALGEHNIVWNSDDEKGKTVGNGIYFIRLQYEDKVI
                     TRKVMHLK"
     gene            complement(63413..63883)
                     /locus_tag="PLB65_00260"
     CDS             complement(63413..63883)
                     /locus_tag="PLB65_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOQ76985.1"
                     /translation="MKWYLIVGLTVMVMVATLLVRIPVPGGGYFNLGDVVIIFSGLFG
                     GWKAGLIAGGIGSALADLIGFPVFAPITLVIKGLEGLVAGLAKPGIRWQRNLYPAIAV
                     VIMVAGYFFGCWLFPNLGLAVAVADLPVNIVQAAAGFFGGKALHIAYTKISKTS"
     gene            complement(63880..64542)
                     /locus_tag="PLB65_00265"
     CDS             complement(63880..64542)
                     /locus_tag="PLB65_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOQ76986.1"
                     /translation="MKPLYRLILVALALVLFFVLFCIVYLESLTIQLAMLLIVILFSA
                     WTFSAKGLIKKIYHLLPFILLLFGVYLIFAVFQIGKSRAHWIHYGLTRTTLLLSSLLF
                     MQVLINWLRIDSFLDFPIGIDKLKYIILGKLLYRIAFSSYSELCLFVDSIPGEQQKNA
                     TLKSKFQKHLIVLLALITYVINEATLKGEMIDERIWHCYTVPNSIPVNKIELEDKIKE
                     SK"
     gene            complement(64526..65050)
                     /locus_tag="PLB65_00270"
     CDS             complement(64526..65050)
                     /locus_tag="PLB65_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ76987.1"
                     /translation="MIEIPQGPIFCWQDFSLLIEKKLTFEPGKIYHLQGENGSGKSSF
                     LIHCLLPVLESKKDLIYRIYLQQLFHLQGYAIKSHSAFYKPELDLRSELDCVQYLLNN
                     LFETFSQDPRPVYCIVDENRYIQEIHRYLKENDFPFCLIYCAHSSFSLSEDVNIVNFK
                     VINPKQSIIYEATI"
     gene            complement(65043..65390)
                     /locus_tag="PLB65_00275"
     CDS             complement(65043..65390)
                     /locus_tag="PLB65_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ76988.1"
                     /translation="MIHKDYLIADADVPLISCDELRDELLIYNIDDSTANQLINQFEE
                     LTGKAINRFYKVNELSGGQKVILMVLLVLYSPAPKIHFKNLMQSLDTKRREAIKNLIN
                     SCGKDIILEENND"
     gene            complement(65410..65500)
                     /locus_tag="PLB65_00280"
     tRNA            complement(65410..65500)
                     /locus_tag="PLB65_00280"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(65464..65466),aa:Ser,seq:cga)
     gene            complement(65509..65973)
                     /locus_tag="PLB65_00285"
     CDS             complement(65509..65973)
                     /locus_tag="PLB65_00285"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HOQ76989.1"
                     /translation="MICFSDRSHYTFMKEAIAEAKVAANEDEIPVGAVLVKNGNIILR
                     EHNRSRQLANPLAHCEKLLIDKILVSEPGFLYDYTLYVTLEPCLMCAGMIILSKIGTV
                     VYGAKDPKAGVVGSIYNVLKDKNFNHHPIVVSGILESECSSLLTDFFRKKRI"
     gene            complement(65970..67667)
                     /locus_tag="PLB65_00290"
     CDS             complement(65970..67667)
                     /locus_tag="PLB65_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ76990.1"
                     /translation="MNRILILTIITFLGTIFLISCAQNSTNIAGSVNGTEISLEDFYS
                     SYRGHYSIFGYQTGRTPTKEEKQKIHNETWQNITRAIILKDYYKKYNISASITEVLDT
                     LSNSIPEHIINSPRFRVNGKFDKKLYMQSLMTDKPENLMPLRKHYQEYLIPNMKLQSV
                     LIEKEMLTPKIRKQVSEIIASNADLELYIFDPSKMDISISDAEISAYYQANLEKYSLW
                     QFYRLGYCSIPVIPDSIDLSEAKNVAENIRNELSSGKEAKDVLNSVDSKNVVVSFIDN
                     GYQKTEELPLTVFNALDGLENGKCSEPVPYEKGYMIYQKIQSTKNLTLFNTIYVQSLP
                     RSVTLLAPETQAKQIVKLALDIGLEQAANEFDIEYIVTPAMSPDSLIVPANDLKGKLI
                     KSLKYAQARDILGPLYSAEQSGWYIFEVVEKQTQDYRSLDEVREEIIEELCKEHRIAQ
                     NRKLVQQWIADNSFPATYEMVKLDNINIDSLWEGKPITNIYYQAIKAYLEKAPLPQIT
                     QKDIIIVPKVISYRPGKTKPNPERIKTIYAQNLPDGWFEDWLDKQVKKARVVIYTKP"
     gene            complement(67664..70303)
                     /gene="mutS"
                     /locus_tag="PLB65_00295"
     CDS             complement(67664..70303)
                     /gene="mutS"
                     /locus_tag="PLB65_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOQ76991.1"
                     /translation="MDKVEENKLTPMLKQYFAVKEKHPDKLVLFRMGDFYETFFEDAS
                     TASKILNITLTTRNKNDDNPVPLAGFPYHSLETYLDKLIKAGLKVVICEQTEDPKKAI
                     GLVKREVTEIITPGAVLDQSLLEGNANVFLASLYFTDFKRKIGLAHLDISTGDFLFTE
                     LDSEELVSELQRFNAAELIVDSTGKEELIKKLPMDKLPTITVFDSWQFQPQEAITTLK
                     KHFGVNTLEPYGAHNKTLGATAAGAALAYVQSLYSSPLNHITSLRYYSLSQYMQLDEI
                     SRRNLELVRSIRYGTKYGSLISVLDQTITPMGSRLLQHWLLHPLLSIEEITFRQDIIQ
                     SCIEKSSHLKELRSILKEIGDITRLVTRLGSLRINPRELISLKSYLYSAKILQNKLSS
                     FMHPQFTVWTQNMGSFEDIIALLEKSINDNPPIPITEGGIFAKGYNPELDELMDLIYD
                     GKSWIARLEEDERRKTGINNLKVGYNRVFGYYIEVSSANKNKVPDYYIPKQTLTNSER
                     FISPRLKEFEAKVLSSEEKIKNLEYELFKELRQNLAESLPRFQQLGEVIAELDVLSSL
                     AYLAWQNQYARPVFTESRELHIVEGRHPVIEKLMESDKFIPNDTDLNYPETSIAIITG
                     PNMAGKSTYLRQVGLLVILAQMGSFVPATEMTLPIFDRVFTRVGASDNLAQGQSTFLV
                     EMIETANILNSATPKSLILLDEIGRGTSTFDGLSLAWAIIEHIQKYKHSLTLFATHYH
                     ELTELENLYPDIKNYNVAVKQWNDEMIFLRKIERGGADQSYGIQVARLAGIPDRVIKR
                     AKEILKNLEEHEISPQGLTATIRKKLVRDVPQIDIFEILVDKASENDPVLNDIKEIDL
                     NDLSPVEAWQYLQKIQNKLLGEK"
     gene            complement(70308..71480)
                     /locus_tag="PLB65_00300"
     CDS             complement(70308..71480)
                     /locus_tag="PLB65_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOQ76992.1"
                     /translation="MIPTRYSVIKRILFSFILISLCTVVYSAVRKEFQELNNLYSNGK
                     LDELSEAISNLKPVNDEERACLGYYNAMLKTKTEDTISAHQWLIERFPKSPYAQKSLL
                     ELGKIYILERKIEEATVYLRRINSTDLIERFYWLGLCSWWQDDFATAINNCENYLRLD
                     PTGEFCESAYYLIAECYRAQKKAYSALTTLIKLQNAKLPEMDEQYFYYRLGYAYELSD
                     KLSDAVNAYRRGYELNRYSQIAYQIEDRLLELKSRNRNIDLSFLYPYPKLQIAIAEET
                     VADSLKTVQKIEPKPAAPIVATQPVSKQDLSIKLKSKPKEGFWLQAGRFSIESNANRL
                     VVNIRLLNIPATYYEDISGGKKSWVVICGSFPDRITAEEAKNLLATNNITTFLVAY"
     gene            complement(71480..72739)
                     /gene="rho"
                     /locus_tag="PLB65_00305"
     CDS             complement(71480..72739)
                     /gene="rho"
                     /locus_tag="PLB65_00305"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOQ76993.1"
                     /translation="MLFEEDLFSLSQLQLSRLAKKIGLPGYTQYKGTELIFKLLEFQA
                     AQEGLAFVTGCLEIMEDGFGFLRFPDNNYNAGRDDVYVSLTQIRRFGLKTGNMISGPV
                     RSPKEGEKYYALLRVEAVNYLEPNSMLNLRSFDELTPYYPTERLNLEFDPNNFSTRII
                     NLFTPIGKGQRGLIVAAPRTGKTTLLQDTANAILSNHPEVYLIVLLVDERPEEVTEMK
                     KILKPGNREVISSTFDESPKNHSAVSEMVLEKAKRMVELNQDVVIVLDSITRLARAYN
                     NITPSSGKVLSGGIDANGLIKPKKFFGSARNTEEAGSLTIIATALIDTGSKMDQVIFE
                     EFKGTGNMELVLDRSISDMRLYPAIDLVKSGTRREELLLSQNELNRMYVLRKYLKTIS
                     PIQGIETLRKKMQATDTNEELLNSMSN"
     gene            complement(72762..73487)
                     /locus_tag="PLB65_00310"
     CDS             complement(72762..73487)
                     /locus_tag="PLB65_00310"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ76994.1"
                     /translation="MNEIIKILSKVDGGNILDAATGKGDFINTLKQHLKSFTQIIGVD
                     TSQRCVDYAQKIFPENNIEIYRMNLEDMQFEDGYFDTVCISDALHHIKNREAVLNEML
                     RVLKPEGLMLITEMYSDGDQTPAQETHIMMHHWVASVDRLLGTYHQKTFTRQELQDIV
                     KKLPLKKVEIADFYVPVDDPSKNCPALIRNCQDIIKRLQTMENTEAIIAEGKELMERI
                     KDIGYAGACRLAITGYKKKISYN"
     gene            complement(73620..74516)
                     /locus_tag="PLB65_00315"
     CDS             complement(73620..74516)
                     /locus_tag="PLB65_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOQ76995.1"
                     /translation="MKEKATLILGGGSALGLAHIGVLSILEEEFEITGIIGSSMGSII
                     GGLYSSGLSSAKIYELAGSFNNAKLFSPFKLDKTIKGIFDGRSLLNLFLTWTEGKSIE
                     ESEIPFIACAYDLTSKSTVLFNKGLYADAMRASSSLPVLFAPYKLNQYIFVDGGIEHP
                     LPMAFSSLLSEDKVIAVNVLPEASRSASFINLESEEETKTKRLSRTEVIFNAVLQNQA
                     YLAIRDIAMFSPDIVIEAGMPNGRPFAFHKAREFYNFGRHQAMETLANYKEPRFMPTI
                     RKHYRNLIQHLPNLNIPVKDNT"
     gene            75076..75843
                     /locus_tag="PLB65_00320"
     CDS             75076..75843
                     /locus_tag="PLB65_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ76996.1"
                     /translation="MIKVDNLSKVFIKKDGTNFYAVNKINFEAGDGEIVCLLGVNGAG
                     KTTTMRVLSTIFQPSEGSAEIQGWDIRKHPDKVRENIGFLSGDTGLYNRLSCREFITY
                     FGHLYSIEDQLIEQRIKVMADLLDMNEFLDKKIEFLSSGMKQKVSIVRSIIHDPPVMI
                     FDEPTAGLDILTARNIVSFIRDCKQRGKCVLFSTHIMREAERLADRIVMIHKGKILAD
                     GTLEELRNISSQIDLDDIFVYYINQHTDEQELRANEF"
     gene            75833..77845
                     /locus_tag="PLB65_00325"
     CDS             75833..77845
                     /locus_tag="PLB65_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit/CPBP
                     intramembrane protease"
                     /protein_id="HOQ76997.1"
                     /translation="MNFKHTLVIFRKELMEVLRDKRTIFTTFILPIILYPLIIVGFNS
                     VMMRQTKVLEERGATIAVQDSVDNEISHRLIQGLVRIKNYTFLPYNESTKQHYAEKDI
                     QCIVTIRDSLGSEGTQFFKIYITYDKSKDQSRIVYEKLSKQLSKVEKELQQERLQISG
                     LSPDFLNLVDIRERDTSSAQKKMGMFLGMFLPYIVIMMLFAGASIVAADLVAGEKERK
                     TLETLLVSSVGRTEIVMGKYLTIITLAMLNMIVNLFSISFSLQFMLANQSKEMSGVTL
                     PINAMLILLIAMIPLATLFAALLVSISTFSRNIKEARSYEQPLLMIAMLMGMVSFIPT
                     IEISNLLAIVPVVNIALLFKAVLINEYTLSHILITIISTLVLDLIAILITVKLFKTES
                     VLFRTEEESGGIKVLKKKPKYALTPYNGIVYFTIALILLYYLGGYWQTKDLYNGLIQT
                     ELIIIALPVFIVLKLLKLKPKEILRFKTPKPAGLILVPFIAISASIIVSIMSQLINTI
                     FPYPEKYTEALTQLFNMNEAPWKVFLAIALLPGICEELLFRGFLIRFFEKYGLQWAVI
                     ISAILFAAFHLDPFRFVPVLLLGFLLGYLALRSGSVYTSMLSHIINNGLAFILVTYSN
                     SSWVKFLVSEGDNIHYWLFIPALVVFVISLYAFHKVTAQGEEVCVE"
     gene            77833..79668
                     /gene="glmS"
                     /locus_tag="PLB65_00330"
     CDS             77833..79668
                     /gene="glmS"
                     /locus_tag="PLB65_00330"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOQ76998.1"
                     /translation="MCGIVGYIGYRNALPIVVEAIKRLEYRGYDSSGCALIHKDELQV
                     YKRQGKIIELERCLPEPNKCSGNIAIAHTRWATHGEPNEVNAHPHLDCKGEIAIVHNG
                     IIENYKILKDRLLELGHTFISETDTEVIVHLIEQYLVSEPTLEDAVCEAMKRVEGTYG
                     LVVISIKEPDKLIAVRKGSPLIIGIGENEHFITSDVNAIIIHTKRVIYLQDGELCIVK
                     KDNFEITTLDKRSVKPEISVVDWDISAIEKGEYKHFMLKEIFEQPITIENAFRGRINQ
                     QLGTARLGGLHIPSFELKKVKQVHLVACGTSFYAGLIGKYIIEDLARIPVFAEYASEY
                     RYRNPIIPEDTLVFVISQSGETADTLAALREAKAKGARVLGITNVVGSTIARESDGGT
                     YIHAGSEIGVASTKAFTSQVTIMTLLAILLGRLNHLSPIQGMEYIKELENIPAKVEKI
                     LAQNEYIRNIAATIKDCTNALYLGRGINYPVALEGALKLKEISYIHSEGYPAAEMKHG
                     PIALIDENMPVIAIATKDPLYDKIYSNLQEVRARKGRLITIATEGDTELAKISENVIY
                     IPDTLTNLQPLLTVIPLQLLAYNVADLKNLDVDQPRNLAKSVTVE"
     gene            complement(80265..80633)
                     /locus_tag="PLB65_00335"
     CDS             complement(80265..80633)
                     /locus_tag="PLB65_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ76999.1"
                     /translation="MKKIFKKLLLLGGTVAAAHFGFKAYKKISGTAKLAKSLPEFLNN
                     VYGEMPEVNVNNTLNSVRIRAFFSQEILDKHTDIDNTIREYIDDFYPEIAKSSVDITI
                     MPKMEETEETATEEEPEEKE"
     gene            complement(80657..81373)
                     /locus_tag="PLB65_00340"
     CDS             complement(80657..81373)
                     /locus_tag="PLB65_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77000.1"
                     /translation="MKRILILISILACAVCQVEAISIYEVQFTSSRGSDGTFPSPYMG
                     KDVTLEGIVTATDYREGGFFISEKLNGPWRGILILDRNAKVQRGDYVKTTGTVSEYYG
                     MTCLQNISQNRVLSTNNPLPQPVLLTTGQLAHPEEAEAYESVYIRFLNVSTMANKSSK
                     NSLMVTDGSGTCFVLCNLFNSQRNSKMSTNAQYASLTGIVVYGFSQFALGPITPADYN
                     ILQPTFIQNRSWGRIKSIYR"
     gene            complement(81637..83607)
                     /locus_tag="PLB65_00345"
     CDS             complement(81637..83607)
                     /locus_tag="PLB65_00345"
                     /inference="COORDINATES: protein motif:HMM:NF026235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HOQ77001.1"
                     /translation="MNISLLVLLLCPVYIMGNYPKLSCYYLDCFMSDQNSQLVSALSK
                     YDLVILDMEMSEINPTALLSIRQLNPNVKLLAYMTCQEISTNIPNPIYQPLRYSLRNS
                     IQDEWWLRTATGEHTSFWQGTWMLNCTPFCPEVNGFTWIEHFADFINNNVLNNPLWDG
                     FFIDNCWSIVPYEIANIDCNNDGIVDNRDWLDSQWILGMEAMLNQFRIQNPTKILIGN
                     GGYEYGQYLNGVMFEGWDYNNPNFEFGYDWSTFMNMYNNIEDTFVPPVMNIIQAMEPN
                     GFLADISHFRFTLTTALMGSAYYAIDLGPSDHSDTWWFDEYDSDLGEPLEGFVNPQIS
                     DQPNLLINGEMNSNLAGWELAFVSPTTGYLQNGFENNNSFAICNVTYADGNDYSAQFM
                     QYNNPNMDFQYGNRYLVTFKAKSAQPRTINFVISKHLANWEWVIPHSIPIELNTDWTD
                     YIFSVTSQNTSLNPDQLRFSFNLGNEPGIVYFDDIGLRLMEEELLMREFENGLVICNP
                     YNITLTAELTHPWYHLNGSQDHIVNNGQICTSVSIPAYDGIILRRTPLAIFDSSENIP
                     QVHMSFYPNPAKDKITLYLEATIKCSGRFCLYNLKGQKLRTEILPIIDTGKLEVPLSL
                     VDNNNRPLSKGIYIGSVKFGEFTRNFKLLVLY"
     gene            83909..86836
                     /gene="uvrA"
                     /locus_tag="PLB65_00350"
     CDS             83909..86836
                     /gene="uvrA"
                     /locus_tag="PLB65_00350"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOQ77002.1"
                     /translation="MKTAIIIKGAQEHNLKNIDLTIPRNTLVVFTGVSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGQMEKPKVDYIEGLSPAISIEQKTTSKNPRSTVGTV
                     TEIYDYLRVLFARIGKQYCYNCGRPVGSQTIDQMIEHLMQNKTGTKLQILAPLVQNRK
                     GEHKDELEMLRQEGYVRVIINGQLRNLEEDIILDKKSKHNIDVVVDRIVIKEGIESRL
                     SDSLELALKLADGIIKIDYPEEKQEVILSAQNACPHCHIGFEELSPQSFSFNSPIGAC
                     TLCGGLGYKLEFDPDLVISDPGLSIMEGAVAPWGRLEAKQHSWTMNTVKNLARAFDFS
                     LSTSWKNLPEIVKQLILYGSKGKKFKNSWQNENGRGEFLMRFEGVIPQLERRMNETTS
                     EEMRRYYLQYIGDKPCPACNGNKLKPASLAVKIAGKNISDITAMSISEAYDFFAGLHL
                     KGNELLIAEEVLKEIKNRLSFLKNVGLHYLTLDRRSPTLSGGEAQRIRLASQIGSQLV
                     GVMYILDEPSIGLHQRDISKLVSMLLQLRDLGNTVIVVEHDEYTIRTADQIVDFGPKA
                     GIFGGEIVASGDLETIMHHPKSLTGAYLSGSIKIPTPEKRIKPDARKLSIINARHNNL
                     KNLNVDIPLGLFVCVTGVSGSGKSSLVNQTLSPYLHNHFYHTSHKVGKMDEIRGIEYI
                     DKIITIDQQPIGRTPRSNPSTYVKLFDPIRQLFAQLPASKIRGYKPGRFSFNVKGGRC
                     EACEGAGVKQIEMHFMADIFVTCEVCKGKRYNQETLSIRYKGKNIAEVLDMDVQEAIE
                     FFDAIPAIKSKLKTLQQVGLDYLKLGQPSPTLSGGEAQRIKLSRELSKVSTGNTLYVL
                     DEPTTGLHFDDINKLLKVLKKLVAMGNTVIVIEHNMDVIKSSDWIIDLGPEGGDEGGK
                     IICCGTPEDLINCPNSATGIHLQYHMQKEKELSSNSLTGLETEMRPRKVSTKRKTDKS
                     QAL"
     gene            86833..88992
                     /locus_tag="PLB65_00355"
     CDS             86833..88992
                     /locus_tag="PLB65_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOQ77003.1"
                     /translation="MSYQRFSYAPPTAGKHSVGIAGDFSSWEIISMEEKGGVHYLALE
                     LPPGVYQYKFVVDGSWILDENNPKKVPDGFGGYNSLLVVEPVTEVITWEDIMAELANK
                     TPEKFYQFFRSKENSYELRFFWYPELSDAIYLLYNNQQIQLAHIGQTPLYEVFYCLFE
                     HCGIFTFRLKIQYQNMFLFFGAEGFSEAEEDITPIEIELKAISIFSIPDWVGHSIIYQ
                     IFPDRFYNGNKDNDPDFREWYYEDCRTPPSSSKILSPEKEYYHMVDDWYDINGLKQSP
                     WQKKGVPDFYSFYGGDIAGVKQKLDYLLDLGINVIYFNPLWQAKSNHKYDSADYHSID
                     PHFATTQEMIDFVKLAHEKGIRIILDVAFNHTGETFWAFRDCVEKGPKSPYWNWYDWK
                     KWPLPKPLPPDFNPKDYYQCWWGIKDMPDLNYDLALPHPEENAVRNIKEAHPNAALVD
                     YLMSTVRWWLIDIGIDGFRLDVPDEVPFWFWELFRKEVKKAKPDAWIVGEIWYEARQW
                     VSPLYFDSVMNYAFFKNPVIEFFILSLLNKEEFCRKIESGLALYPFHSLKAMMNLLGS
                     HDTPRIFEISKGDVKRLKLAIAFQMCFIGAPHIYYGDEIAMEGGKDPDNRRPFNWNWE
                     NNPVAGDLRNFYKELISIRKAHPTLCEGHFAFIPAPDKIIAFQRELKGEKILVFLNTE
                     AESIAIDIPESSILLLGKIAERNYILPHSALILSELK"
     gene            89176..91320
                     /gene="asnB"
                     /locus_tag="PLB65_00360"
     CDS             89176..91320
                     /gene="asnB"
                     /locus_tag="PLB65_00360"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOQ77004.1"
                     /translation="MCGIAGIYNFSKETVILTEVTGKKGILNTEPAGLSNDIDNLVKI
                     EGGYRQNGAKAILLKKMTEAIKHRGPDDEGYLIKDKNGIRSYSGMDSTEEIKSLYPLF
                     NSEITTLDNLSKKAVGKTVGAENIILGMGFRRLSILELKPIGHQPMYDKELGIAICYN
                     GEIYNYLELREELKKKGYKFCSQSDTEVIIKAYHFWGENCVQHFNGMWSFSLWDEKKN
                     LLLCSRDRYGIKPFYYAVQNGVLYWGSEIKQLMLTPIDKTLNQAMIWRSMKINSMLVY
                     DDETYWQNIHCLKPGHNLIVTNSKVIVKQYYDLDITSFETSQLSFNEAVEQYRNIFLD
                     SIALQLRSDVEIGATLSGGMDSSAIVCSAVHQKKEPLKTFSSYYAFPPELDERKWIEK
                     IIKETSCASYLISPKANDAIDLWEKLTFLNDLPLAAGFVSQNAVLQEAHKQGIKVILS
                     GQGSDELSAGYRHSLYRYFADLIRGVHISRLGKELPGYLKKDNRAFSKLSKIVLSTFL
                     PESSLYNLEFCYYRFEPFTRSFTNEAEISCGEQILKKIVDIKASRLSNFLYNMVHTTS
                     LQTLLHFEDRMAMANSIESRVPFLDYRLVDFVFSLPSQYKVKPPYTKVIHRAAMKELI
                     PEEIYQRTDKGIFSSPFYQSWMKQELKPYISDIFASSTFRQRGLWNLPKINHFWHKYL
                     GGDNSQVEMLYNVVALEIWFQQYENQSTGGLK"
     gene            91317..92201
                     /locus_tag="PLB65_00365"
     CDS             91317..92201
                     /locus_tag="PLB65_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77005.1"
                     /translation="MKNIVKIIALCFVLISLSSCKLFKSPTVTKIQDVKVVSITPDKS
                     TIKISLMVNNPNCYKLKLDKLEVELLNKDRDKVGYANLEKVVEIPKKAAMCINFNVEL
                     ETRPVVRMISSLNQKVQFFIVGKGHGKVKGLSKKFYFDEPYELDLKEELKNIIPKFSV
                     GTQNLFKITKTSVEKMSIGQTELHIHFIILNPYGLTYNLKGFPAEIYIDDKLAGTGNL
                     KNQLKFNENIFSQDGIMVFQLNNLKSIFGAVKGAIKGEIKYSVKGNVIIDAFGMQIKQ
                     PYSYQDSLPVNIVELILN"
     gene            92356..93951
                     /locus_tag="PLB65_00370"
     CDS             92356..93951
                     /locus_tag="PLB65_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77006.1"
                     /translation="MKKYLFLVLIILPVFLLGQVSSEETNIPGDSMPEEESEYPEGTQ
                     WGMGGAVGTVTIDNRTYSQIRLQPELQIGKFGIGLDIDLIIDAEGNVRKEDWDELQDY
                     VNKFLYIRFAQRNDPFYFKIGSIPSYTLGNGLIFNNYSNMILYPSVKNVGGYIGVNTN
                     ISGLGFEAYSHNIRKNEILAGRVHIQPLAATNIPLLEKLRLGVNAGIDRDQNAKYEDK
                     DGDHIPDIYDKFPKDKMRYLDTDNDGIADEDDIDIDGDGILDHPDYNPYIAQNFPGIE
                     DIAQGYNLDIVIFPDTAAAYNKKDEILIYSADYQLPLIETDHFNLVNYGELAKIDGYG
                     TGMIFPGFSSKFFIFDAKVEFRNFGDQFIPGYFDKQYDQQRAEVKHIVLEESGSQYWF
                     LTTKESNLENVEASLGWFGYLRANLFDFIYPKIAYQDMYGKNNFLGKSLWGSITINPY
                     MIPKLKEATIYYSQTNTNYINFRYLRNENAYISGRLVYSLSDNANLIGKYSEFYTDIN
                     CDGKIKGNDEVIESFTFGVEFQF"
     gene            complement(93955..95121)
                     /gene="manA"
                     /locus_tag="PLB65_00375"
     CDS             complement(93955..95121)
                     /gene="manA"
                     /locus_tag="PLB65_00375"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HOQ77007.1"
                     /translation="MPYLIKPSLKNYSWGDIKYIQELIGALDMTGKPLAEAWFGAHPQ
                     SPSLIITEEGEIPMDKLLETYPESMLGKNQGNNLPFLFKILAAAQPLSLQVHPDRQTA
                     QYGFQKEEEARIPITDPNRIFKDPNPKPELICALTPFIAMCGFRPYEEIIRNFQILGI
                     DNLWQALSSFQKKPCSKNLQELFNHILASEPSQLETTLSAMENCKNNISPKFRFTCQI
                     CKHLNEFYPQDSGVLSPLLLNTFTLQPGQAIFLEPGILHSYIQGAGIEIMANSDNVIR
                     GGLTPKHIDRDILFAITRFETYLPKLLHPHLKLNELVPYPNPAEEFHLYRLILEGNYI
                     LTNQYKPMLVFCFRGSLNTESGLSLQRGKAMFIPSEEKMLQISGSADLFIASTY"
     gene            complement(95133..96473)
                     /locus_tag="PLB65_00380"
     CDS             complement(95133..96473)
                     /locus_tag="PLB65_00380"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005935796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HOQ77008.1"
                     /translation="MNKVKVRTWYLMLASLYFYYKTSGSFIILLIITSLINFGLGALI
                     YRAKSKAGKRFYLILSIVWNLGSLGYFKYTNFVISTVNQIFDGQLPLYDIVLPVGISF
                     FTFQTWSYTLDIYFGKLEPLKSFVDFTFFVSFFPQLVAGPIVRASYFIPQIHKKLSLD
                     KDTIAKALVLIFSGLLKKGVIADYLSINFVDRIFENPALFSGVENLLGVYAYALQIYC
                     DFSGYSDIAIGLAALLGFSLPVNFITPYRATSVTDFWRRWHISLSTWLRDYLYIPLGG
                     NRKGKPRQYLNLMITMLLGGLWHGASWNFVFWGGIHGIALVLDKMILKLKIMQTKVMK
                     FVGVIVTFHFVCFGWIFFRSPDFSVAWLILKRIFTDFHGNIFFQWISQYHICAALILL
                     GYIFHWMPEAYNNNLEKFFRKCPLVLQSLLLAFLIWVLFQFRSAAIQPFIYFQF"
     gene            complement(96606..97904)
                     /locus_tag="PLB65_00385"
     CDS             complement(96606..97904)
                     /locus_tag="PLB65_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOQ77009.1"
                     /translation="MSKRFFLSVLILALAFLMLQNNSRAEDNITVDNTTELFTEEEYP
                     SDMDTLEEFIQSYYLLLESNEYLKQFKFINPEKNYISGDKKALQSFYEKLLELRSGLR
                     DRVTIYQIGDSHIQSGYFSGTARTSLQKYFGNSGRGLIFPLRLAGTNQPDDYRITASG
                     AFSRSNSERGLSGYALNANNGMCLEITTNNFFKGDNSFNTVSMITAMPGLKPKIDLAE
                     KNIKEYSIKAGNYFYSRVSWDKAVTKIKLTLAGEPSTLFGISLENNQPGLLYHSCGIN
                     GASFYNLTAQSTLFEQIGLLNPDLIIISLGTNDAQGTYRNEVFSKNLISFMDKLKQYN
                     PETPVLFTLPPDSYKRGKHNSDLDKVEKVIIDYAKENHYAWWNLSIVMGGNNSVQKWR
                     NQQMAANDMLHYTPKGYMLQGYLFYQAFIKSYKSFSEKAG"
     gene            complement(97905..99296)
                     /locus_tag="PLB65_00390"
     CDS             complement(97905..99296)
                     /locus_tag="PLB65_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77010.1"
                     /translation="MNKVFYRPLIIVVIAVILVELIARVIPDSDNKIFPIKKLSWLES
                     IEPKDTIEPTDTIVVKEATAIPNNLLPLQPFIQKMNNSAKSIRIAYYGDSIIEGDLIT
                     GKLRQELQSVHSGSGVGMIGITSIVSGFRQTIKHKFSRNWESISFMSTGRNNIPLGIT
                     GYTYIPRNYYIAEKVIEPLQLDSLAVLDTSQVVSPKPHKESERFYVDYNPWVEYTATT
                     TAGGAPNFKRIRLFYSHAADSSFVKVSYDGSPFVVRYLNNQSGVQMLDLSPAVSCKKV
                     RLEFSAYDPLHIYGVSFDEPTGAYIDNYPIRGYSGMFFQRIRSDILSGFQKHLKYDLV
                     ILHYGVNVSNYQIRDYSYYERGMKKSIAHIQKALPGVPILLISAHDRSIKQGSEYITS
                     PDIPYLVATQSNVAKETGCGFWNLFAAMGGINSMPKYVNHKPPYAGKDYTHFTRAGAD
                     HIAEMLLIYLRGH"
     gene            99634..101322
                     /locus_tag="PLB65_00395"
     CDS             99634..101322
                     /locus_tag="PLB65_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ77011.1"
                     /translation="MIFRSNRQKDDQLTGWLKIWCMLIFLFLSGFLLAQTRTVKVGIS
                     QNEPKMFIEPSGKPAGIFIDILEAIAAKEDWKLEYYEGSFDAIYYRVAKGDLDLMPDV
                     AISPTREKDLDFHKEPVLSSWSQIYTRKGLEINSILDLDGHSIAVLEKSIQQNVIESW
                     IKGFGLKVKIIKVHNFDAAFAAVANGIADCAVSNYLYGRRHCKQYGLVDTGVVFEPAT
                     LYFATTKSKNQDLLDAIDKHLLTMKKVPNSAYYAALKRWVSKDEGFMLPAWLKFTILI
                     VILALVLTVIYGLTLKHQVNIRTLELRKSNQEMEQRIIERTAELEKAMLKAKEADMLK
                     SAFLATMSHELRTPLNSIIGFTGILLQELPGHINEEQRKQLTIVQNSAHHLLDLINDI
                     LDISKIESGQLDLHYESFFLNDSIDKVVKIVMPQAEKKGLELKVEISQSELEVYCDKR
                     RLEQVILNLISNAIKFTETGYVAIIVHSEEDKYQIQVQDTGIGIAPEDLEQLFLPFHQ
                     IDTGLTRQYEGTGLGLNICLKLINMMGGTITVDSIPGKGSIFTVIMPYKKEFTG"
     gene            101319..101696
                     /locus_tag="PLB65_00400"
     CDS             101319..101696
                     /locus_tag="PLB65_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ77012.1"
                     /translation="MKELVLIIEDNPENMYLMRYLLENHSYEVEGVITGPEGIEKAER
                     IVPDLILLDIQLPGMDGYEVARQLRQNPVTKTIPIIAVTSYAMIGDREQALEAGANAY
                     MEKPINPDTFVADIEKIMENIIK"
     gene            101707..103779
                     /locus_tag="PLB65_00405"
     CDS             101707..103779
                     /locus_tag="PLB65_00405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ77013.1"
                     /translation="MKILIVDDHMNNNYMLESLFKGYGYEVITALNGKEALIKLQTEK
                     VDLIFSDVLMPVMDGFTLCREIRKDEKLKHIPFIFYTATYTGTKDEEFAEKIGANAFL
                     IKPADPRDIIDTLERVIEQSKDESFEISATAEYEQEVQKLYSERLVRKLEQKMLETEQ
                     EALARKQAMKLLQRNERLLRATQRVSNLGGWEWEVACKKMYWTDQVYLIFDLDLNKYK
                     GIEDRKMYELIMLCYNETDRKKIKSSFVNCHINAIPYSIEGWITTAKGNKKYICTVGS
                     PITKKGKVTNVYGYFQDLTNRKYAEMKEHELNEQLSQAQKLITIGQLAGGVAHDFNNI
                     LTVIMGNAEIALSQISPDNPIKADIEEIMKAGKRASNLTKQLLSFSRKQIVKPEPILL
                     NHILEDMTKMIRRLINENIEIELNCEEDLAFIKSDVVQMEQVILNLAINAGEAMPKGG
                     KLTISLKNYIPDSAFYKEHPDIRPSKYVLLTISDTGIGMDEATQKSLFEPFFTTKSNQ
                     NGTGLGLVTVYRIIQSTSGYIKVKSAPGKGTTFLILIPATEEIPEVIPIEAPKPKVAG
                     HLETVLVVEDDEAIRFLTSKAVTKLGYKVLTSVSGDDALIKIRHQELTPDLIISNVIM
                     PGLNGIAFASEIKKYLPGVQIILMSGYSDNFLSQYGTITPDIYFLAKPFTRSELEEAI
                     RKQLSLDT"
     gene            103899..105557
                     /locus_tag="PLB65_00410"
     CDS             103899..105557
                     /locus_tag="PLB65_00410"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77014.1"
                     /translation="MKQNTLLVVLLIFAGALMGNPPEWAWMQRTFCTGNCIIYNLKSD
                     SQDRINVTGYFEGELSLGAITLNSAGSRDAFVAQLDVEGNWLWACSIGGTGAEYAHKI
                     AVDAEDNIYVGGLFYQDFTVGTTLLENNGNSDIFCVILSSAGNILNAIAVGSEDQDTL
                     KGLAVTLDGILFLSGNYRYSITIGSTNLTNAYVGLFISKWDNQLNPIWAQQASSVYGC
                     PECANLGTDNFGNCYITGYFLYQCAFGYPNQVILTSQETSGYIVKLDVNGNAIWGERI
                     SDGGMGIMDSYIDPFGNSYITCDIFFIITDSMQNNRLDAFTYCGKLDFNQNWEWYNDD
                     SAMGHCIPTKICGDETGNCYITGKLWGNYTFGDDTLTGYHNNANIYVAMANPQGQWQW
                     GLQTYDAGYLFYLNIIDITALETGGCVITGSNSTDSLHFGDFFIPPSSNRYSFIVKVN
                     GETQAISDPGIESSPQIVLIYPNPSHDIVRFQLKNSEGLTSEASIYNIKGQLVKSFPN
                     RQNSGVYVLNWNGCNETGNKVASGIYILSIKTEKGIIKKIFTRL"
     gene            105940..106539
                     /locus_tag="PLB65_00415"
     CDS             105940..106539
                     /locus_tag="PLB65_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ77015.1"
                     /translation="MSALPNKKKLILEMATQLFTRFGFAKTSLDEIAAAAQIAKGTVY
                     YYFPSKEDLFIASIEVKTREYFTMLRQHLNATEGFEEKLTEFLRFPIKLIYENMPILI
                     ECLINIPQSFQKQLHQHRERSREVMMQILSEIIQLGKKEDLIDDRFSEDRLCEVITDW
                     FVIGEPGLKIVDVPRLLEMIERDNDFIIKMLLYGIVKRG"
     gene            106543..107922
                     /locus_tag="PLB65_00420"
     CDS             106543..107922
                     /locus_tag="PLB65_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOQ77016.1"
                     /translation="MKKTLIVLALLTFILSSVYAIDLENSITKAKQNNKELLMAQEEI
                     RKSDESYKQVRGSAYPQLNLKGAYGLSKTYYPDSAIPSKMDISAGLDDSPTNNEEYLA
                     GVINSVVNSMIPTSPAEAGSFSMQLQMNQIVYSGGKLGSGIKAGNYYRQLQRINYKII
                     EQNVVLKTTELFYQCLLAQKLWEVQEDGLQIARRHLERVELFNQEGQVSEFDLLQARL
                     GVAKLEPQVLKAKNDYDIAVSAFRKQIGEEDSDIVPEGEFVLPERIEITLEDATEQAL
                     KQRNEIELIGISTQMKELQYKVEKINYLPNVALSADYSLYTSADEYAIQNDDFGHKYG
                     ISLGFQMPIFTGLTNTAKRDYARYDYQKAKLQQRDTEELIALQIKQDYQKYYHAWENH
                     NVQTQNIHLAERGLQLAQVRYDNHVGIQLEVFDAQLTLQTIKLQYYQSIYEIISADRN
                     LKKSIGIAL"
     gene            107940..108965
                     /locus_tag="PLB65_00425"
     CDS             107940..108965
                     /locus_tag="PLB65_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ77017.1"
                     /translation="MKRYLVILTVLILILTFACSKKKEEGKSMEQIYSEEGIPIRQTV
                     VEPSTFRQNLLYNATLNGMEETTVQAMVSDVITRINAKVGDRVVKDQIIVCFPQNTPS
                     AQYEQALTAFNSIKTTYERMQRLFAQGAISQQDLDNVETQYKVSKANLEVSDKMINVR
                     APISGIITAMNVNPSERAYPGKDLFTVASTNGYKAVVMVPDTEITKFRKGAKATATWL
                     DTTISGRVSEISLAMDSSTKAFRVEVAFPGMNKKINYGVTAEISIEILTKPNVIVVER
                     HQIQSNNGTKYVWLNQDGKAVKREITTGLDNTLAYEVISGLNPGDILITEGLNLLTEG
                     AKLRVIE"
     gene            108979..112062
                     /locus_tag="PLB65_00430"
     CDS             108979..112062
                     /locus_tag="PLB65_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOQ77018.1"
                     /translation="MFLTDLSINRPVLVTMAIMVFIVFGVLAYFNLPLNLMPDLKLPY
                     VVVQTVYSGAGPREIESQVTEPIEEAVATVSQIDFMQSYSMENVSIVMVAFKLGKNID
                     VANQEVKDKVDAVIRSLPDGTDRPIVLKMDITAFPIMDLVLSGNMPPKDLYELADGEL
                     KDRISQIPGVARVSLTGGAKRQIDVRLTDKVVFENNISLSQLSGILAAANLDMPAGNF
                     THGTQEYSVRLKGEFQNVKEIENTDIPTAFGVKKLSQLARVSDTTEEVRSKAIYFNIQ
                     ENLKDDNIVRLSITNASDGNVVSVAEEITKQIPILNKGLPEGVKLEVMRDDSVFTKDT
                     INSTLVNILLGIIFTGLVLFIFLHDLRSTLIVAISMPYCIISTFVFMQLFGYSFNIMT
                     MMGLSTSVGILVSSSVVVLENIFRHKDMGKTRKQAAQIGTNEIGTAVLASTLTNIVVF
                     LPIATMTSMVGRFFTEFAITVTVATLFSLLTAFTITPMLASKIIPKEKKVSKWGQSFD
                     KMFDKFSNVYSKFLSYVLRSKKTSVSILLFTLVALLISVGLAFVVGMEMIPNVDQRNL
                     TVSIEMPQGSNLEETAKTMDIIQNRLAKHKEIVHILTNLGSSGFIDTGTNLASADIKL
                     VDRQDRKYSANQLVDILTKELADIPNAKIKIAGSSMGMGGGGRSAVEFMLEGQDIDRL
                     EQLKNEVIKAIEDIPGLINLDTSSRWGRSELTLYPKRDKLAAAGATVYDLALALRANV
                     EGLVSTEYRESGNQYDLKISLEDDVVNTPDKIRNLNVVVMGRNYLVSQLADVDFSSGI
                     NRITHYDRYKTVMVTGDIASGYHLSDITSQINERIQKFNFPSGYRFEWGGEAKMMQET
                     TVDMVRTFILAVILTYMLLSAILESFAQPVLILATVPLALIGVVLSLLIAGQTFNIVS
                     MMSIIMLVGIVVNNAILIMDYVNIKRRDGYSVHDALMEAGKMKLKPIVMSTLAIIFGM
                     LPMAIGIGSTGAEMTRPMGIVSIGGLVVSTFLTLIIIPAFYFITTKNIHAKKESQ"
     gene            112077..112592
                     /locus_tag="PLB65_00435"
     CDS             112077..112592
                     /locus_tag="PLB65_00435"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 domain-containing protein"
                     /protein_id="HOQ77019.1"
                     /translation="MKRITRLISLALLAIFILVGIFGCYGNMSLTKKVYNFNGSLGNK
                     YVQSIVNWAFWICPVYEASMILDICLFNTIEFWTGSNPLTMNENEKIIKYAEGPNGEY
                     KFEISKNQIIISQPNDGTGNENTVLSFDPDSNSWVMNNDGVSQQIGYLEGDQLKLVSP
                     SGEELSINLIK"
     gene            112602..112829
                     /locus_tag="PLB65_00440"
     CDS             112602..112829
                     /locus_tag="PLB65_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HOQ77020.1"
                     /translation="MIERQKIESILDKVRPSIQADGGDVELINIRDDNVIEVRLKGTC
                     NGCPMATLTLKAGIERIIKEEVPEVKEVIAV"
     gene            113238..114188
                     /locus_tag="PLB65_00445"
     CDS             113238..114188
                     /locus_tag="PLB65_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOQ77021.1"
                     /translation="MKNILVLGAAGQIGSELVPYLRKIYGANSVVAAYRSTPLTLEIQ
                     EGGPCENLDAMDSKKLFEVVKKHKIDSIINLVAVLSAKGENNPLMAWNINMGSLINSL
                     EVMREVKGAIFTPSSIGAFGPSTPLDNTPQDTIMRPTTIYGISKVAAELWGDYYYLKY
                     GVDARGVRYPGIISNVTLPGGGTTDYAVEIYYEAIKNKRYTCYLKPGTYLDMMYMPDA
                     LRACVELMEADPNKLVHRNSFNVTAMSFEPEMIAAEIRKHIPEFEIDYKIDPVKQSIA
                     DSWPNYMDQSAAQQEWGWKPEYDLSTMTVDMLNVLSKKLK"
     gene            114188..115180
                     /locus_tag="PLB65_00450"
     CDS             114188..115180
                     /locus_tag="PLB65_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOQ77022.1"
                     /translation="MVKIGVVGVGHLGQYHTQKYQSIDNAELVGVYDTNFPRAQEIAG
                     KLKVHCFSSYEEMLAVCDAVDIVATTTAHYELGKKALLSGKHIFVEKPITSELAQAQE
                     LLEIAAAKNLLIQVGHIERFNPVILKTENEISDPLFIESQRISSFQPRGTDVSVVLDL
                     MIHDIDLVLSFVQSPVSEIRASGVGIFTPTIDIATARIEFVNGAIANVTSSRVSMKQE
                     RTLRFFQRDAYVKMDFLSKQVRIIKKSDNVDKLLPLILSGVVDINPEQLLDIQNYDVG
                     DTEKDALTLELESFVDCVYNNKKPIVDGLAGTRALEIAIKITNQIQAQNAKLRA"
     gene            115196..116491
                     /gene="asnS"
                     /locus_tag="PLB65_00455"
     CDS             115196..116491
                     /gene="asnS"
                     /locus_tag="PLB65_00455"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HOQ77023.1"
                     /translation="MQDVFIKDIDKYIDKQVRLKGWVRNIRHSGKLLFIILRDGTGEL
                     QTVAFQPDLGDEKFELAKRLTLESSVIICGIPKPHPKKENVYELAVSDVMPIQIADEY
                     PISKKEHGPDFLLSNRHLWIRSQKQWAALRIRHSVYYGICNYLNEQNFIRFDSPILTP
                     NACEGTTTLFELEYFDEGKAYLSQSGQLYLETGIMSLGRVYDFGPVFRAERSKTRKHL
                     TEFWMMDAEAAYVEHEENMQIQEELIRYVIRYVLSNCKLELEILERNVEALKAADAPF
                     KRITHYEAVEFLRQKGSDITHNKDLGAQDEVLLTEDSAVPVFVERWPQEIKAFYMKRD
                     PENPNLVLGDDLIAPEGFGELIGGSQREDDYDLLYSRMVAEKMPIEDYQWYLDLRKYG
                     SVPHSGFGIGLERLVAWISGVPHIRETIPFPRMIYRIYP"
     gene            116638..117852
                     /locus_tag="PLB65_00460"
     CDS             116638..117852
                     /locus_tag="PLB65_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOQ77024.1"
                     /translation="MITNYFSETTKTMKSSLIRELVAETKNVPGLISFAGGFPSPATF
                     PKDILSELYKEIVAQQGRDVLQYGNSEGDDILKDELLKWENYPNLNRNNMIITVGATN
                     AIYYMTRCLIDPGDVILCEAPTFLGTIVAFEAIGAEVQSIPMDNEGIDLNRMAEKVKQ
                     LRDANKNIKFLYTIPDFQNPAGITMSIQRRRDLLNFCEAEEILIMEDNPYSRLRFSGE
                     ELPTLFRIAHTEMNNSWLVTEIVSFSKILGPGIRVAYVKSHPELINKMVSWQQKVTIS
                     PDCVTERVVAKFIADGYMEPHIKSLCEFYKPYLDKMLTCLETEMPSYVQWTKPKGGIF
                     LWLWLPEDVNADTLFYKAKDHLVTFIPGSKFYPEGQEKFNCMRLNYTYSTFEQIEKGI
                     KHLGELVKSIYV"
     gene            117915..118361
                     /locus_tag="PLB65_00465"
     CDS             117915..118361
                     /locus_tag="PLB65_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77025.1"
                     /translation="MKKLILITTLSIFLLPLMAQIEFPRPNLNPYSSNGGNSLLNLEK
                     LSMSHSMGFEAGTSSTGYGYYLSRYTNHLKYNFNPKLELDLDLNFVNVGGFNTSSKFE
                     FNNDNSSRVIPEFSLSYKPRDNVQIQFQMLYGPYLNPYKDSWYRSW"
     gene            118396..119133
                     /locus_tag="PLB65_00470"
     CDS             118396..119133
                     /locus_tag="PLB65_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HOQ77026.1"
                     /translation="MAVLGAALGSFFNVLIYRIPKKQSIIFPGSHCESCKKAIPIYQN
                     IPIISYILLRGKCSRCGAKIHWHHLLVEIITPLLFLGLFFRYGLHDFRFYKYILLASF
                     LIPIFFIDAINQIIPHVLSIPLLISGIILAIIPGNDVGIVNALLTGVIVFSLLLLLAW
                     AYQKLRGVEGLGGGDIWLLTGLAIYFGLTGIPYIFLLSALMGIIYFLIFVKDKSKPFA
                     YGTFIAVAAIIWSVIGEDTIFNLLSFL"
     gene            complement(119355..120572)
                     /locus_tag="PLB65_00475"
     CDS             complement(119355..120572)
                     /locus_tag="PLB65_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ77027.1"
                     /translation="MSIIGIIRIAFKSLARNKTRTFLTMLGIIIGVAAVITMLAIGQG
                     AKKIVEDQISSMGTNVLMVTVNFTRSTTNVRQAAGSGTLLELDDVFAIRSQVEGALYA
                     SPVYNSFAQLKFESQNWRTGIMGVDTDYFYIRNMNTEKGELFYDSDVENGTKVCVIGK
                     TVADNLFGDIDPVGQIIRIRNIPFTVKGVMESKGQNVMGGDQDDSVWAPYTTVYARLL
                     GRRWRNMMIVVSAVSKDRIPEVQQDILELLQNRHRGTTSEEFLVRSQTDIAETASKVS
                     DTMTILLASIAGISLLVGGIGIMNIMLVSVTERIKEIGIRMAVGAGKRDVLLQFIIEA
                     VAISILGGFIGILLGFGASAIVGNAMDWSIAVTPFSVLISVGFSMAIGIFFGWYPASK
                     AANLNLIDALRYE"
     gene            complement(120569..121321)
                     /locus_tag="PLB65_00480"
     CDS             complement(120569..121321)
                     /locus_tag="PLB65_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ77028.1"
                     /translation="MSDLLIRTENLVKTYQMGEIQVHALRGVSLEINKGEFVAIMGAS
                     GSGKTTLMNLLGCLDLPTSGEYYINNIGIHEITPTELTKIRNQMIGYVFQNFYLLPRT
                     TALENVELPLLYRNKMSHHQRIRKAIEALTTVNIANRAKHFPNQLSGGQQQRVAIARA
                     IVNDPMFLLADEPTGNLDTRTSLEVMTIFQKLHKEGKTILLVTHENDIARYAQRLITF
                     RDGRIISDVPNKNPQNAEYDLAHLPKLPGDEE"
     gene            complement(121323..122615)
                     /locus_tag="PLB65_00485"
     CDS             complement(121323..122615)
                     /locus_tag="PLB65_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ77029.1"
                     /translation="MKKYLKYIIIIALIIIAIIVISSMRKAKNKPEFRTVTPSFGNIK
                     EVVTATGSLNPYVLVSVGTEVSGKIDKLYKDFNDRVKKGELLAKLDTDILSTNLEAAK
                     ADLAKAKTALDQAKLDYDLQKELLEKQMTPEYDAKKALYAYQTAQQNYNNAQLTLQRA
                     QKNLANAYITSPIDGIVVSRDVDAGQTVAASMSSPTLFQIANNLDQMQISANVDEADI
                     GKIIVGLPVEFTVDAYPSENFSGTVKQIRLNPTTESNVVSYSVIIDAANPEQKLLPGM
                     TTNVTIIIQSRENVMRISEAATRFKPSKEVWEQFGLKWSDDLIKQAQKEAYASLASAM
                     SSSSNTDSIKVSKNTSGKRTQKGSANNVSSGKLALVWVLENDVPKAKGIKTGISESGF
                     IEVISGLNGNEVLITGVNSKKNSDTTNGTQGGPGMRPF"
     gene            complement(122651..123895)
                     /locus_tag="PLB65_00490"
     CDS             complement(122651..123895)
                     /locus_tag="PLB65_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOQ77030.1"
                     /translation="MKVITVILMLLPLLLAAQSYTLEELISFGLENSFSVQKSSLSYQ
                     SSRSMLNTSKWNLLPDASVTGGVNKDFDPAAGTDDVSSSAGLSISKTISLNDAAYFNY
                     LYASLDTKTANMQLEQTRRTYAYNVFSAYIKVLSSQKQRSSLEENLLIQTRVWEQSKI
                     LLQLGKITPFEVKQNEIEVLNSNIALMQLDNTIANARKELFALVQMQDEGYNLTELDL
                     DIQKELPAFNTENNADIQILNQELKRLNLQLKQNKLDDFPRLNLAYNFDRTVSGADFD
                     FDRYNTNHGISLSLSYPLLNLFENSESRTRTKISRQLSQVSIDEKKDQLQRNYDSALR
                     ELQYLKRMNELYSEQLAQSRDQITQAEERYRLGLIELLELDKTRTNYIETDISYNNNL
                     YSIMAKQEEINYLLSEPVLGKW"
     gene            complement(124102..124548)
                     /locus_tag="PLB65_00495"
     CDS             complement(124102..124548)
                     /locus_tag="PLB65_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77031.1"
                     /translation="MKVNFKYGLSGYTGKADDLVFCYDRISGKIYARKRVYPRLTEEN
                     ARIANISRNIFALQPSEVYKNDLRLYLLRYNGLKVNRYKQLRSWVNLYHLLMANMAKL
                     YPEIDLKTITREQIYQENLPCISVKKAVEAGVLPQVNGYERMEALI"
     gene            124978..125979
                     /gene="argF"
                     /locus_tag="PLB65_00500"
     CDS             124978..125979
                     /gene="argF"
                     /locus_tag="PLB65_00500"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOQ77032.1"
                     /translation="MAFNLKNRNFLTLMDFTPKEIQFLLDLSMELKKAKYAGTEQQKL
                     KGKNIALIFEKDSTRTRCAFEVAALDQGAHTTYLGPTGSQLGKKESVADTARVLGRMY
                     DGIEYRGFGQEIVEELAQYAGVPIWNGLTDQDHPTQVLADFLTAMEHLNKPLNEMVFV
                     YCGDGRNNMANALMIGACKTGMDFRIVAPKSLFPDQNLVNKCLEVAKETGAKIAFTDN
                     IDEGVKNADIIYTDVWVSMGEPDSVWEQRITLLKPYQVNSEMMLKTGKSTTIFMHCLP
                     SFHDLKTKIGKDIYDKFGLTCMEVTDEVFEGPQSVVFDEAENRMHTIKAVMVATLGS"
     gene            complement(126049..128943)
                     /locus_tag="PLB65_00505"
     CDS             complement(126049..128943)
                     /locus_tag="PLB65_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOQ77033.1"
                     /translation="MRKMFSLLLIILAAGMLNALPKFIIGIPASELSLSKEENSLRNK
                     VKELSAQDLEIYYYNEFYIIAGTNVSNYPKAVNLGTPQDGRFYLVTIGNRHPDFDRNI
                     VGEVLLKMGSVVLLKTDLDEIQLRQQIIYPFIPLLYEPIKIADYSTLNNSLKHTRTDI
                     VDMIADVSVDSVAYFIQSLQDFMTRYALANNRLEVANWIKAQFERFGITDSQLYPFNW
                     SGTTQYNVVATIPGTIYPDTYIIVGGHHDSITNDNPMTFAPGADDNASGTVAALEMAR
                     VMMANNYQPKCSIRFVTFAAEEFGLWGSKAYATMADQNNLDIRLMINHDMIANISPNP
                     WDSRVFLMPYDGYLDLTEYAAMLTSQYTTLLPVYGSMNLSASDSYSFWQHGFPVVYFF
                     EYNFSDVYHSNNDIVANLDPFYCTKVIRASTAVAVSFADMPSTPTNFTVQDEGTGSSI
                     HLSWNPVSDPTLSHYKISWGITQDTLSDSQTTIQNEISINNLTTGQLYHFALTAVDDN
                     DNESMPIFNQAVPRLIPSVPVSFTDHPLPQVVELVWQANTELDLAGYKLYRSLDSSEI
                     GELIATLPPTVQSFTDNNVQGGDFYYYYRLCAFDNDNNNSPYTAVIKTRPINMNYGVL
                     IVDETMDFSGSNLFQPTDEQVDSFYDQLVAGLWSTSHFDLATLTDTLRLCDLGIYSVI
                     IWHGNDYTDMQYPYYIKDALKSYIYYGGKVLFSLYHPSMAFEMNSGYPASFPSGSFIN
                     EVLGISNVDYSTSARFNKAVSTDPNYIEVNVDPAKVPPGLNGHLFHIEVINPNAQATP
                     IYSYASAYDHNTLQGVLNGGVVGILNNYYAGKTVTLSFPLYSMDFNQSRQVLRYIVET
                     CFEQPISVSDEVINQVPEISILPNQPNPFSSTTQFNILCKNISAPLEVKIYNLKGQLV
                     KTLYKGIADSKNKISWDGTDYKDKQTASGIYLIKANQGNTIETRKILKLK"
     gene            129245..133039
                     /locus_tag="PLB65_00510"
     CDS             129245..133039
                     /locus_tag="PLB65_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ77034.1"
                     /translation="MKKISFMLILVFAAALLMAVSGTIKVNDNPAKVQLLENNKNGLS
                     VHYALDEIQFRDINTKEGIFTELISTNYTSTNTTGLPALPLMRQLISVPLGAEVSAYF
                     TTINSKTINLTEQGINYPLMPRQESVSKSADIEQLPFIVNRDFYNSNTWTDNPSISVT
                     ELGIMRGTRIFAVDFVPVKYNPTLKQIEVIYNAEVKVTFSGADYAATSELRDKTYSAA
                     FENVFANTLLNYYEEPRSSLLRYPIGYVIIVPDNFISPMQPFIDWKKQEGYNVIVAPT
                     SITGTSTTTIKTYMQNLWNAATTENPAPSYLLIVGDIAQVPTNNGSTSSHPTDLPYVR
                     LQGNDYLPEMYFGRFSATTVDEVTNQVNKTLMHEQYTMPSDTYLNEVVMIAGVDSNWS
                     PTHANGQINYATNNYFNTAHNITSHTYLYPASGNSASTIVQNVSNGIGYINYTAHGDV
                     TNWSNPSFTISNVNSLQNTNKYCFTVGNCCLTNKFDTAVCFGEAWLRANNKGAVVYIG
                     GTNYTYWDEDYYWGVGYKPPIVGTGSPFVAGHTGAYDALFHDHNEPFADWAGNAGSMV
                     FMGNMAVVQSNSTRANYYWEIYAIMGDPSITPYLGIPAQNAYNPPAQMFLGLNSMDIQ
                     TDPYSYVALSMNNELHGVGLADATGNLTLNYAPFTEPGMAKLVITRSLRKPLIADIEV
                     IPNEGPYVTVGTLELNDGNNNIAEAGDNINITLQFNNVGIEAATNLTATLTCDSQWII
                     LNNATVQLPDITSGNSITQTNAFNVNILPNVPDQHIAAFTITVTDGTDTWETQRNLTI
                     NAPNVVFGNYTLFDGDGDGIYEPGETVTVTLSISNTGHMNVDSGSLAIVMNNPYAALD
                     NYGFIIPPLTVGNGIPIVFYLFIDQNCPEGEMIPIGFAMNAGIQMLNHSIIIPVGIVG
                     ESFESQSFTSYPWVNDSANPWTIVSGSTNVHFGNYAAKSGAIGNYGSTSLQVTIDIPE
                     DGDISFWRKVSSEATYDCLKFYIDNNETGSWSGEVGWSGVSFPVTSGNHTFKWTYSKD
                     VSQVGGNDCAWLDDILFPGIGVLETAIFYTTTEQIDFNAVLPNTTVSADFTLRNLGNI
                     PMQGIISTPVEFELSQNGNPLSDDYAYQIEAGETQFFTISYVAGNTVSTIHTNLVITT
                     NDPTHSTVEIPVNLTPLANADNNAIPIVTTLQKNYPNPFNPETTIRYSLSEAGPIKLD
                     VYNIKGQLVRKLVNEPKAAGNYTVVWNGKDEQGMNVSSGIYFYRMQTKNYSSTQKMML
                     MK"
     gene            133148..136834
                     /locus_tag="PLB65_00515"
     CDS             133148..136834
                     /locus_tag="PLB65_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ77035.1"
                     /translation="MKRILLFLCLILSFIAISAEQIIVSNYANDIHLTNSNPNNMELE
                     FTLGSFFREAVNIDGEQWFHLFLKKEGITLDEGLPQLPVLARSLIIPPTAAMHLNITN
                     SEHIELAMPIAPSKGNITRNIDPATVPYKFADFYQSEESYPAEIAYLTEPFILRDYRG
                     ITVRFQPFVYYPATGILRVYTKLNISVYAQGSDLTNALTSPKSSYSRYFESTYQNMFL
                     NFSTAKYPSLNEEGRILVIKHSMFDTAIQPWVDWKRQLGFTVNVVDVTEAGSSATGIK
                     NFIQAQYDLNDGLMFVQLVGDGPQIPTLTYSGGGSDPSYALLAGNDNYYDIFVGRYSA
                     QTAAELETQVNRSIYYERDIAPGATWLEKAIGIASAEGGSGQGDMGESDIAHMNNIRT
                     DLLNYGYTTVDQIYDPGATAAQVTTSVNQGRGFINYVGHGADTYWVTTNFNNNNANAL
                     TNDYMLPFIVSVACVNGNFVSQTCFAEAWMRSVNETTNAPAGAIAFWGSTINQSWAPP
                     MRGQDEVTDLLVGNLKYRVGGLLFNGAHKMIEVYGSQGLGDARTWTIFGDASLMIRTK
                     NPQVITATYNPVLFLGMSNFTVQTIPFARITLTGNGIVYGQGIADASGNCVINLDVIP
                     DQPMDLALTIYAFDYQTYIQTIQVLPSTGPYIMVTETTFNDNDDNVFTAGETILMGMA
                     LSNIGSEIASNVSVTLSSTDQYITIINPVLNIGNIASGDASSIEDFQIQLANNIPDEH
                     LVELTVTIATIDGDVFEYNRSFIVYAPLITWGALQIDDSTGNNNGRIDAGENVTLTYT
                     VTNSGHCNASDISTDIVIDGVPYLIAPIISTIDNLAIGMSAQLIYNITFSSQIPMGTP
                     VQLTAMTMIGDYVFVNIYTVIVGIVMENFDYNFSNFPWVFEGGNWTIAGGSYNGTPAA
                     KSPTINHNSSSSISINFECPQASEISFWKKVSSELNGDYLKFYINGVLKYQWSGTDDS
                     WSQVTFPVNPGTNVFKWEYTKNASITQGSDCAWLDDIIFPSTGENIGTPIFVIDIENI
                     DFGQVTVNTTASHEFTICNCGTASMLGTISTEEPFSLGMPSMPSQYLEYIILPGEAFT
                     FNVNFRPTENIEYNGSLIIHTDDPNALINNIPLYGTGKPVANEDPVISVVTSLHGCYP
                     NPFNPSTSISFSLHEKSRVELVIYNILGQKVRTLVNKPLEPGEHKVTWTGTDNSGRPV
                     ASGIYFYRMKAGNYSETKKMILKK"
     gene            complement(137045..137596)
                     /locus_tag="PLB65_00520"
     CDS             complement(137045..137596)
                     /locus_tag="PLB65_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOQ77036.1"
                     /translation="MKTVLQVFVLLVLSFILFFLFYNALTPHKYVDDQNTFCDKIVKD
                     DSAPKQAEADNIKIQDSELIKNPAIISDKIAIDKSATIEPKNDKPKNETSITDALPEN
                     AISNSEESSQELTNANRIVYQVDKDICIGCKLCLRICPVGAISIKKGKAVIDQDKCTG
                     CGTCAIGDGKRFKGCPVAAISAF"
     gene            complement(137597..138394)
                     /locus_tag="PLB65_00525"
     CDS             complement(137597..138394)
                     /locus_tag="PLB65_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOQ77037.1"
                     /translation="MRIKNPVHTLRFIVQLLFLLISMLFLAGIIVCGLPYTIHNLCPY
                     SAVCFGFSKNFLCSLNSSAFGLTIAFSFLILVLAIFFGRLFCSFICPLGTIQEYIFAL
                     FNKKSKKKKQVPHYLERRFSLVKYGVLAVTAILAIAGISYIYIRFCPIYAISMLPYIA
                     VLGLILALLIIIVGIFIERFWCRYLCPYAALLNLFQYLGKVVGFPRFKIHRNLERCND
                     CGFCSLNCPMNIDLTEEEYVNNLDCILCFRCSKKCPKPDTLVWKKDK"
     gene            complement(138611..141391)
                     /locus_tag="PLB65_00530"
     CDS             complement(138611..141391)
                     /locus_tag="PLB65_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HOQ77038.1"
                     /translation="MPKQDLKIKGEKTVDVKEIKKKAQLQKAKEPQKTTKEYQKPAAR
                     VIIDDESLRPTNIVPVKWNRWIAFGVFLITLIVYMCTQARTMSFWDSGEYATCISILG
                     VPHPPGNPFYILLGRAIVAIFGWAVPHGMIAAFISGLFSALAVMFTYLITIQLTSMMK
                     VKSWEAIFAGLIAAFYTAFSFTFWMNAIEAEVYSGLVFFVNLIFWLTLIWVQKSRDFS
                     HQNFLLLIAYLFFLGFCVHQTALQIAPAMLFIVCYPLLKQGIKSGSFWPKLIVYSIFL
                     LIGYFFFGVIGKQMQIDDLDKMGFALVAFIIVFIELKKVLDRRIWLLGIALVLVGLSS
                     HIYLMVRAAERPFINEGNPSNWKMFQEYVLRKQYGNTSFVQRRGNFFTDQLNYHFLRY
                     FSMQWLNEPFFTKFIGVTSNMLKPVANIFIAFLGVAGAMLQFRKNKHSFWYFLSVILL
                     TTIVMVFVMNLSNAEVRDRDYFFVVAYNMWAIWLGIGALTLVTLWKSKPVRIAVLILM
                     CLIPLSNFITQYRVHDRSQEFIALDYGLNFLNSLEENAIIFTNGDNDTFPLWYAQAVK
                     DPYSKENIYSARDVYPNAESQEAVAKAMEYKNKCLKGIRKDVSVANLSLLNTAWYIRQ
                     LRDKEGILFNIPDEQLDDLSIRRVEEPLVIPGPPENPAMGFTMNIPPTAEWRKDEPFY
                     RISDLAVMQIVKDNYGKRPIYFAVTCESFVNFEDYVRNEGMVSRVVSNPGDSMIDPQR
                     LLHNIDVVYNYRSIDNKKVYKDDNMNRLIMNYGSGFVRAANYFVDLGDYPKALEYINK
                     ARRFVHEEIKLTEFYTNYYSKTGQWNKLDEFINKTIAPHPEGWKIYLSYILAYTVDKY
                     PDKVLPYLKKGFLLFPDQEYFAHLALGYAQEYRQYDTIKQLLTEVQPKLTYDVSSFIS
                     DLTSPPPMDTIP"
     gene            complement(141366..142352)
                     /locus_tag="PLB65_00535"
     CDS             complement(141366..142352)
                     /locus_tag="PLB65_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ77039.1"
                     /translation="MGEKLRMTIIYAVLIICGLAMIIPFLWMLSTSLMTQSEFNKQES
                     IFIPRVTYYIWDNGTQEQRILLVQEKGENSIIHILNEEDKIIEEYKEVPSSQVKKITK
                     VPYFHWQNYVKAFNKVPFAIYFKNTILVSLFTLLGVLITSPLAAYAFARMEFFGRDFF
                     FYLFLSMMMVPEPIYLVSSYVLLDKISWLDTYQALIVPWCVNIFTIFLFRQHFKSLPQ
                     ELFDAAAIDGCSTFGMLWRIMLPLSKSVIATASIFSLIGSWNSFMWPLVMTNRPELRV
                     LQVGLSYFNQEASTQTTLLMAASTFSIVPILILFFMAQKQIIASYAKAGLKD"
     gene            complement(142364..143296)
                     /locus_tag="PLB65_00540"
     CDS             complement(142364..143296)
                     /locus_tag="PLB65_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ77040.1"
                     /translation="MKAKWKISPYLYIFPALFLLMAFRLIPIVMSFIISFFDWSIKGT
                     GKFIGLMNYATMLKDEVFWRSMGNTIWLVIIVVPASIILSLLFAVLLNNIKALKGLFR
                     TVYFMPYVTSLVAVSIVWKLMFNEQTGLINTLIGYIGISPQKWLSESRGIFEMLFSGL
                     GINLHGWIGGPSQALFAIIIMTVWKGLGYNTIIYLAGLQNISKVYYEAADIDGASKIK
                     QFFKITLPLVSPTTFYVLIMTTIVTFQTFSQVYLMTDKGGPLNTTKLIVYYIYEKGFD
                     TLEMGYASAVALALFIIILALTIYQKRLEKHVNY"
     gene            complement(143293..144726)
                     /locus_tag="PLB65_00545"
     CDS             complement(143293..144726)
                     /locus_tag="PLB65_00545"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOQ77041.1"
                     /translation="MNETSKPRILIVDDSVETLELFELQLKNKYKVDTATSLKAGKEL
                     LDNYGYHIAIIDLILPGENGLDLIQYVTQSYPHTAVIAISGQASIETAVAAMKLGANE
                     YLVKPFRDLDIINIQVEKILQTQWLIAENKRLSALIDTDIETDLIIGNSPAIQSIMQT
                     VRKIAKLDTSTLITGETGVGKSVFANLIHRNSLRKHQKFVTVNCGSLTETLLESLLFG
                     HKRGAFTDAYRDKIGYFQEANGGTLFLDEITETSLSFQVKLLKVLETGFFRMVGGEED
                     VHTDVRIITATNKDIGECVANGTFREDLYYRLNVFKIHIPPLRERRDDIKVLAYAFTN
                     EFCNKYNKGELKLSPEVMSILLNYSWMGNIRELKNVIEHAVILAEHNVILPEDLPENI
                     VNSTNMISHAFQQEEYGDWLSAKQNFERRYLNQLLSQCNGNFSKASKISGITRENLYN
                     KCAKLGIDYKQFRTRNNLQKETKETTA"
     gene            complement(144723..145298)
                     /locus_tag="PLB65_00550"
     CDS             complement(144723..145298)
                     /locus_tag="PLB65_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77042.1"
                     /translation="MKDLPPAILEGLIHNIRTPLNLILGYVHRLKQQVNCDEAEQIYK
                     AGIKLEDLLQETWEAIQIRGGGNSKTPLNTWLDAELNFLHNYLEIKHRFIFTPHYYEK
                     EILGKITPIALSEWFESFLMFITEHIPEGQISMQIFVNQQGLKIFINGSNYLADISDL
                     NLPPEFFSFNQDIKVDLKYENAKLVTMVKLI"
     gene            complement(145300..146496)
                     /locus_tag="PLB65_00555"
     CDS             complement(145300..146496)
                     /locus_tag="PLB65_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOQ77043.1"
                     /translation="MELSKRAREIQASPIRKLNPIANATKAKGIKVYHLNIGQPDLHT
                     PPEMLKAYHEFSDKILAYGPSQGLDLYREGLVKYYKRCGIEVKPDDIIVTTAGSEAIT
                     FAMMVVCEVGDEIMVPEPFYTNYNGFATMAGINLVPITTYAEDGFALPDNSTIEAKIT
                     NKTRAIMLCNPGNPTGTVYSNDEIFRIAELAKRRGLFFISDEVYREFIYDGLTHTSVL
                     DVPDFEENAILVDSVSKRYSACGARIGCIVSRNKELMASVLKFAQARLCPPTVDQLAA
                     NAGINLPDSYFEQIRTEYQSRRDLVMSELDKIPGIVCRKPEGAFYINVKLPIENSEDF
                     IIWMLKEFQIDNETVMAAPGDGFYATEGLGKNELRLAYILCQEDLKKAMSIFAKGLAT
                     YQRIHK"
     gene            complement(146719..147420)
                     /locus_tag="PLB65_00560"
     CDS             complement(146719..147420)
                     /locus_tag="PLB65_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOQ77044.1"
                     /translation="MKGVIIAAGFGSRLWKTTKQIPKTLLPYKKGTILSTIIQQLQMA
                     GISELIIVVGFKKDYIANYLQNDPPTLPVTLVENLEWERGNALSVYKAKDYINNEPFL
                     LSMSDHLVKMEALKKIVEVPERINLLLTDPFISENFDVDDATKVLTEDDYIIAIGKEL
                     DNYNALDCGIFRLEPDFFTAVEKSVQKGNESISNAITELISLKRIKVVMLNKANQWMD
                     IDTPEAYNFAHNFVI"
     gene            complement(147424..148530)
                     /locus_tag="PLB65_00565"
     CDS             complement(147424..148530)
                     /locus_tag="PLB65_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HOQ77045.1"
                     /translation="MKKIKLLFRIGYAYHKAAFDPIIDLLLNDEKYDIWFSLDMEKIK
                     YFIFEFPYRNRIIEDWKNLGYRFTNETKGFDIVISGDTLRNAEDYGKTLLIFLNHGTG
                     IKNILYRNLARTPGVKYQIFVEGKHRVESLLKCPYLEKSEVHLIGLPKLDFYFQGKFK
                     REEILERWGLDPAKKTVLFAPTYKPTCMYEIKDDIFDQTRNYNLIIKLHPYSWMGKYA
                     PHKQHRIFEKRVKKYPHSVLLPFTEFNIVPYYAAADTVLSEASSTVFDFIALNKFGIV
                     YDLDCDRLNHSDGQPLLEIDNREFLKGAFPHIQNGKQLQTAIEKALNPTKEMKTKADE
                     YRQRFFYGLDGKASIRFVEKMEQLYYEGGHENGA"
     gene            complement(148598..149917)
                     /locus_tag="PLB65_00570"
     CDS             complement(148598..149917)
                     /locus_tag="PLB65_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HOQ77046.1"
                     /translation="MLRGNEIKPAEGKLGVLLVGLGAVSTTFVAGVQAIKKGISEPYG
                     SVSQMSTIRLGKRTENRTPLIKDFVPLADLQDIEFLAWDIFESDAYEASLKAGVLDKE
                     LLDKIEPELRSLKPHKAVFDNKYVKKLHGTYVKSGTNKMNLAHQLMEDIKRFKEEKNC
                     SRLVMAWAASTEVYLEADPIHNSIESFEQGLKDNHPAIAPSMIYSYAALQMGIPFLNG
                     APNLNLEIPALRKLAEEKKVPVGGKDFKTGQTLMKTIIAPGLKARMIGIQGWFSTNIL
                     GNRDGEVLDDPDSFKTKEVSKLSVLETILEPEKYPQLYKDLYHKVRINYYPPRGDNKE
                     SWDNIDIFGWLGYPMQIKINFLCRDSILAAPVLLDLILFIDLAQRCEMSGVQEWLSFF
                     WKSPMTAPGLYPENDLFIQQMKLKNTLRYMMKENLITHLGLEYYNEM"
     gene            150388..150699
                     /gene="trxA"
                     /locus_tag="PLB65_00575"
     CDS             150388..150699
                     /gene="trxA"
                     /locus_tag="PLB65_00575"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HOQ77047.1"
                     /translation="MIKDLNEVDFDKAIAGDKVIIDFWAPWCGPCKIFSPTLDEIDKE
                     VADLTIYKVNVDNYPALAGRQNVHSIPTLLFYRRGKLVDSSIGVVSKSVILQKLERMN
                     D"
     gene            150779..151666
                     /locus_tag="PLB65_00580"
     CDS             150779..151666
                     /locus_tag="PLB65_00580"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOQ77048.1"
                     /translation="MPNHKLKNRIEYVSFRIALAGLKLLPPEAAKAVLKGLFWFGGWV
                     IGIRKNVALQQLSKIFPDKNSKEKAAIIKKLYRNMALSIYECYLMDDETLYKNIRIEG
                     REFIEEALSLKRGAIFATAHYGNWEAARILPKAGIPISGIAKPQRNVLFDKYTNTIRE
                     RCGMRIINMKRGLRDIVHEFRENRIVAILIDQNAGGSGLLMNFLGFPASHWKGVAKLS
                     LRYRVPIVPCFARRSEDDRIVFEFFPVILHNDWDDKEENYKSVLEEVNKILEHQIREH
                     PEQWFWVHLRWKHSYDMLA"
     gene            151707..157190
                     /locus_tag="PLB65_00585"
     CDS             151707..157190
                     /locus_tag="PLB65_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ77049.1"
                     /translation="MFIDHRYEVLEHLGTGAWANVYKVRDIRSGKLYSLKLFQYVSSD
                     DLYSRFSAEEMHHITRLEHPNLSHTVDFGHVADHIYYISEYFEGKPLTGVKFTKAKLG
                     QIYDIAIQICYALHALHTQNILHKDLKLENVLYRTEGNTIILKLIDYGFSKVDTTKDS
                     QAVSGTLPYLAPEIYLGKPASKASDFYALGVILYKLTTGSFPFSVDQINALITGGNQY
                     FIPSFPSELNKDIPLSLEKFILRLLDQNPDNRFASCEEIVHYINRTQSFQYPFSVSWS
                     MINTLHFNSYLVRDKYSHQILDYIPAVEKGNGKIISVTGGDGLGKDSIISLFRYHLLS
                     GKYFIFDYTCSKSDHEAFFALIKEFVQSLSPEELQEYSGLKTISEKFRCYLFGSEQEA
                     KTVSQTSSELKLDFESVKSLLIDLSINKPIIFIIRNFQYVHHNTIDFINFMSSYIVKH
                     RIMIVITCNDYNKINQIDHTVLMNIPTLNLDESRNYINRLLSGFSSSDNRDSVPEELI
                     NEIYRRSAGNPYFIVEILIDLMQRRKLYYDKMLHYPADLSDYQLSSRLLQSIHSRLSH
                     LKDVNYLHLQKLSVVQTPLTRELIIYILKIKDSELYSLLNDGIYHEILKKQGKNYYFT
                     FQEAKQRLFNECRDKTHKLVSKRVLKFYADEIITDFETCRGLIANAFLANDLICARLY
                     YLRYYNMLNQENEQEQAFFAILNVLNLDFNPELSISLKDLISDVKKLQEKMEFTGFYE
                     RAKFIIQELMNLPDFFEKYYLLGTINILAENIQKALEYFSTGERNIITGRQQLLLWLA
                     YAQVFAKIDPSKMILYVDKALKQDMTLELHIAFVDRLAVYHSLMKNWDMAIKTIADFL
                     EKLPPEHNTDVMYRLIVIHNDLGVFYSNQKNIDEADEHLFTALNISRRYNINRYLGLI
                     YNNISDLYLKQGLTVISEHYSELAYKYSDELNFAMSKALSLLNQGEAKIKMGDFVTAE
                     QRLLECEALVLSKESTNYLQSVKRNLALAKSKIKGFGHYFSFIMDNEPELISCRIKEL
                     NPLVKTYFYYLHEIGNAKKLRRLLSKNVHINYKHIHEEEFYHNALALASMAEKDFEGA
                     LNELKIALRHAGEINNNYAIAVFYVLQIRCYYGLKDVERARELTELALPKIKENHYRY
                     WECELAVLALKIDLLVPDIPLRSILRKVNEYLDAWSNYEYYQLNVELYQVKIQIFIEL
                     KQEELAEAEYANYKKYLENITENIRADDRQNYLSINLYYLNNLRKFNLLPIASRTKDL
                     MKKWNEMLFNITNINSIDRIKFLIEKGLIQVFSPWQFYLMKYSSKVSSYTCFQSYNTG
                     KENTFNTELLPYIERAFKIDNLVILEQNNLHFMVVPFQIGSKRIGFLLLSDKSEMPFT
                     KQEMSLMRDMKQHLAALLVRIEDFSQITLRIQKMNQLMAITHELMRIVNINELERAIV
                     SACIDFTNSTRGFLIKRDSDGAIIYTVQLNAAKQILPTVSGVSKTVLSLSQNTQEIVS
                     TFNAVEDNRFKSAISVQDYVLHTIFCAPIIVDNNVYGYIYLDNLNDNNREMYLNTEII
                     KLLLEQITIALKNAMLYENLLQKNNELNTFETLKDEFMAIVSHELNTPLTTLQGYVTR
                     LKRNLFADEEEKKDIINKIENSVKKIILTINDITTMNTYNLAKSLPFSTLSVQEILEI
                     IYQEVQILSRKRKMFIKLEIEKDLPPIKANWEAFHLMIYNLVLNAIRFTNDFGTVIIG
                     ARKSAFQSEKIDGKESLVIYVQDNGIGIPEYQLKNVFRKFYELNEIYAHKSGTIEYRS
                     SGLGLGLATSKRIAELHSGKIWIKSKENEGTTVFVTIPIKQSK"
     gene            157207..160128
                     /locus_tag="PLB65_00590"
     CDS             157207..160128
                     /locus_tag="PLB65_00590"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ77050.1"
                     /translation="MKRYIIMMLCLVLMCICFAESVGKKSSPSLLAVSNSETYAYRYI
                     VELYDAGDAYNLSKEITLFKATYPESVYLPYIRLIEGNLAMESGNYTKAIEIYELLLN
                     IDLVQNVRNDILLNYAYCLSELKQYDRAFNILQRLDSETVNPVYIELSNNLRGDIYFH
                     LGQFYSAEKAYKRAMVAFPDNEGIRFSLFSTYLALDKDDDALELLKTITPESDYYYKY
                     IGSWLGYLLDNELYEDFDRFVEEQKLEDITSNLDIIDIRIRRALHIGDFEKTSQLLQK
                     ITIESPQFQYYQALVLINAGKEKQADSLLALLVSSARPEISVPAYMERVKLLFKKNPK
                     AAIEQLNSYLKQTPSDFMKAELYYTMGFFYYNTGNYSEAIRHLGIARHYETSRELCSR
                     IDILIAEAFYASGNNDLAKDTFNRYLNLYPYGSRRDKAYFYLGYMSFQEKDYTQAKVN
                     FQMLQEQYPNSPYCNDATFYLAEMDFYLANYNLALENYLYLYSKKVDNEVVALRITQV
                     YYYLGDYEKSESYIANLVPSYDTCILKGNIMLVKKEYEKAIDQFLQAEKYAPDNLKTI
                     EAKSYRALCLYQMKHFKEASALYLQLSGQKESPDTYLFLAAKSAYAAKDYHLALELYN
                     SFIDKYPESTHFLEALSNIANTYYNMGNYDRAINDWINILTRFRNQTKFTDNQLATIQ
                     DALIGLELALQYVNKEDLLAELLVLPDTFFSEYIKFELNYILVKVYANKKLWADLIAR
                     AEKMKEDFPQYRQEDVELLMATALIELNEYTKADSLLSELYAESRNNDTLLKWAELEF
                     ITGNYESALEKYKLAYKNNPNSEIWVNMLKCSEANNYNEFDHLWTLGIDFLENCPEVN
                     VFRIRQLYYTEHFEEANQLAEHLINYSLSTFDHATAFLVMGMINYRKGEYQTAISTLS
                     KVVMLFPEHKDICSKAINYIVRSLLDSGAYTEAEVFFIQHENELTITDKTELTELLEG
                     I"
     gene            160128..161606
                     /locus_tag="PLB65_00595"
     CDS             160128..161606
                     /locus_tag="PLB65_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77051.1"
                     /translation="MKRFFLTLASILTLSLLIAQTQELPDLTISGESSFKPYLHKHSL
                     LFDHSLVMGDSLPAFIPPAVSTNENRSESYTMQHRCYLQFEGNVDYGFNSFISYYPTS
                     EVLNALTYTLDMCSPVSKLLSVHNKLFLGTEFSSKLPLSFRLQNVSSHADDFDNTLLD
                     FTVSHHRPIVSIGDFILKDISAQLGYSYLYQQNLGSSYKGNYLNLYLASRLDVDPVDI
                     KAKILTREGDVGFQLSTNAKDEYMNISRLGVSVLADEYTFIPSLEFLYRSPIQKFGVV
                     SIANEPLLESNTFLSHMEENPWIAFDDGHKLKKIPLNFKVGLEYLYPEKMNFTLAYLT
                     IKNNSRYEIDSPVLINSINYSIPTVRYTDIFSNLTIIEAFFKLDEVNIHQGMELDLSY
                     LSEDDYRRAPYRPLLNLDSRFSYNFSRWFFSLDILQKYFTVNHLGNDLPEAVIINIGA
                     EYREANYAVYFELANLFNRKQYVFSELPAQGRNFYLGLKHRF"
     gene            161672..162511
                     /locus_tag="PLB65_00600"
     CDS             161672..162511
                     /locus_tag="PLB65_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77052.1"
                     /translation="MKLKVLNLIREILNVLFGDYNGIQVFIAPVTLVYWIDSNSLITS
                     ATSLISFRIHYLPLLAFFTILAFVVFMLVKIKLLYPCSGSEFIDLTIQLNVSVMALIL
                     IALIIYALSNFLAYFYGIKGTVKSGLYLLFKLYTAILILYHYLINVVLAPFYRRRYSH
                     LHALKNFLVWARHNKFLLFRYVLLIVMVIFFAVRVYQIVLQFLIIPAINSISDFTGFD
                     LKFKLYPFLGVSDIFTNILAIFGAFTVSNLLFFPIIWVLKYLVDRFLPFSNLIRSNYA
                     QIT"
     gene            162495..163784
                     /locus_tag="PLB65_00605"
     CDS             162495..163784
                     /locus_tag="PLB65_00605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOQ77053.1"
                     /translation="MPKSHKKKLRKIKRRRKNNNLGVFILVGFLACFIIFVIIREPIN
                     EQVGKKTDTVDQKQDMPSRSKKRNKKEGKILTHDKKSTSVSDRKALGTNQIREEKNTP
                     EVAIVNTIGKLGIPKSYYRKRNTEEYTIYNVPIDKSIMDLVYANMIFKGELESNGAKF
                     IKGTDNPGKQTLLFNFPNVPKQYQINIYYDSELYKNKQTRRTITLVVDDFGPVGGDLL
                     EGFLALDKEICFAILPDETYSVLTMEKAKEQGRTTIIHIPMEPIGYPEVNPGKNAILV
                     QYEDRQIDKVLSHFIEQLPYCEGVNNHMGSLATTDEEVMSAVMATLKKHNKFFLDSRT
                     TNISIAYAIAQKYHIKSYRNDLFLDSPNISQSTMDAKLNQIIELSNNNKNVIAISHCH
                     NFEKLQYLKRIINRLKAAGFTLVPITEIDKYNVPEIL"
     gene            163794..164609
                     /locus_tag="PLB65_00610"
     CDS             163794..164609
                     /locus_tag="PLB65_00610"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOQ77054.1"
                     /translation="MSFFQAIIMGIIQGLTEFIPVSSSGHLVLFQYFFGMGESENIVF
                     ELFLHLGTLFAVLIYFRKTLWELFKSLFCWGNTVNREQHRKNRTLVYYLIISTLATAI
                     IYILVGDYFKAAFAMPILVAFFLLITGGIVFLSDYFVDQGIPASNVGFFRAVIIGIGQ
                     GIAILPGVSRSGTTIAYSLATGMKRKDAAQYSFLLSIPAILAGNLSEYKALTELDSTM
                     LQNYLVGFVSSFIIGYLVIAFLIRLIENSRLKYFGIYCWIAGLLSIILLALGI"
     gene            164609..165610
                     /gene="holA"
                     /locus_tag="PLB65_00615"
     CDS             164609..165610
                     /gene="holA"
                     /locus_tag="PLB65_00615"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HOQ77055.1"
                     /translation="MEAPIFALDFAAANVNLGSSYLLVGTDSYLVDKVIETIKKKLKK
                     KENIDTFIIYGDDVKSSELADILDTYTIFSSAKLVILKNAEQLNKYELKTLSSYFSSP
                     SEIQSLAIATEKIDLHNESWKQIKAHSTIINCESPRYGGAIRSWLDKSLKDMNKTMTI
                     KAIEEFINRIELDYYYAANELNKLDLLTTGRNRITEEDVMKSLGTTRTGTLIDFYRAL
                     GKKQEKQAIEALEKMLNANWEFLQIFFQFTKFYNNIWHILLLKKAHISDNEIVSKHLS
                     DIYPNQRKEFLELSRSYNLASLEKIFTILLETDQQFKLSVADSQILLITCLIRVMEA"
     gene            165610..167073
                     /gene="malQ"
                     /locus_tag="PLB65_00620"
     CDS             165610..167073
                     /gene="malQ"
                     /locus_tag="PLB65_00620"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOQ77056.1"
                     /translation="MHKTGILLHISSLPSDFGIGDFGPEAIRFAEYLKTEGYTYWQIL
                     PITHCGYGNSPYNPLSAFALNPYFISPELLYQQGLINQRMLKAVKIPASNITQYERVY
                     NLKDKMLAQATDNWMLTNNLDDYIEENAFFLKPYLTFLSLSRIYDDSCWYNWNEEHKH
                     YSEALYQKTRQLDSSFINIKAATQAMLSEQLKYFKEYLHKMGIILVGDLPLYLSYDSS
                     EVWAHPEFFDLDEQGRRLHFAGVPPDAFSEKGQLWGNPLYKWDVLQQDSFKLFMNRIG
                     MALSYLDILRLDHFIGYVNYWQVKPNFDEKGHPILPQNAINGSWEPALPEEFFSTLLK
                     NYGIERFLAEDLGILNQEVCRVRDSFGFPGMIILQFCFENSVPEVKKYPPERWLYTGT
                     HDNHTIRGWFESLPSDSLSLKHLQEYCIQNKITDIHQMINSENIHNIMRNIALSSGCQ
                     QIIFPLQDILGLGDEARMNVPGTALGNWQWRLEERIY"
     gene            167101..168708
                     /locus_tag="PLB65_00625"
     CDS             167101..168708
                     /locus_tag="PLB65_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOQ77057.1"
                     /translation="MPKLMLLGDEALAQGALDAGISGFYGYPGTPSTEIIEYAQKSKQ
                     AAENNIHRTWSSNEKTALEAALGMSASGKRAMVTMKHVGLNVAADPLMNSAITGASGG
                     LIIAVADDPSMHSSQNEQDSRYYGNFAMIPVLEPGNQQECYDMAFYGFELSEKYHIPV
                     LLRLTTRLAHSRSGIVTKEPIPQNEMRKPEDMFQFMLLPAIARKKYQHHLELQKDFMR
                     ESLNSPFNAYKTEASSKKLGIITTGLAYNYLREAFKGEEIPYPVLKIGQYPLPEKQIH
                     QLYDSCDELLVIEEGMPVVEEQLKGLAFPGLKPIHGRLDGYLPRTGELNPNLVAKAFS
                     LPDTIGRPVPEIVVGRPPALCVGCPHSDTFLSLNEVMAEYGRGHVFSDIGCYTLGFMP
                     PYNSINSCVDMGASITMAKGASDSGLFPAVAVIGDSTFCHSGITGLLDCIYDKANVMI
                     VILDNATTAMTGGQNYTGYGKLEDICLGLGVEKEHIRIFVPLKKNYPEITQIYKEELA
                     YNGVSVVIARRECIQTLKKKNKMETVE"
     gene            168705..169274
                     /locus_tag="PLB65_00630"
     CDS             168705..169274
                     /locus_tag="PLB65_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOQ77058.1"
                     /translation="MKKDIILAGVGGQGILTIATILGTAAIKRGMNLKQAEVHGMSQR
                     GGDVQSHLRLSDDPIWSDLIPYGSADMILSVEPLEALRYLPYLKEDGWIISNSTPFKN
                     IPNYPPEEEIYREIKKIPNYVLFDADTLANAQKANRSMNIVVLGAAITHLGFSQEEIE
                     EAIKAIFAPKGEAIVEANLRALLAGIETK"
     gene            complement(169513..170343)
                     /locus_tag="PLB65_00635"
     CDS             complement(169513..170343)
                     /locus_tag="PLB65_00635"
                     /inference="COORDINATES: protein motif:HMM:NF016816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HOQ77059.1"
                     /translation="MKIYISTDMEGMPGTFNWEQEKTNRPEVMKNYQAHISDCLNAIL
                     YSGFNEFIEEITVADSHSGGDNLSYDITSLDKRISLISGGPRPQYMMPAFSSEYSMVF
                     LLGYHSGTGALKGNMDHTYSNSKIFKIWINDIPMNEAIINSAYAGYLGVPVALISGDK
                     ALEEEITTAMPWVHFVRTKEAVAKFAAKNYSSIVVKEALVKAVQASLAKKDIPLYTFQ
                     SPIHLKIEFHSTSMTDCAALMPHTKRLDGRTIEFIDDDYGVIFEAIMALVTLAYGSIL
                     "
     gene            complement(170340..171257)
                     /locus_tag="PLB65_00640"
     CDS             complement(170340..171257)
                     /locus_tag="PLB65_00640"
                     /inference="COORDINATES: protein motif:HMM:NF016142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HOQ77060.1"
                     /translation="MHSLLNYSSRIKKALDLGKPILAMESTVLTHGLPYPDNLDILET
                     LEELCYSLEVEPATIIVLDGQLHIGMDDQEKEILKNKLQHSQELNKFSMRDIPYAINY
                     KKSGGTTVSATMLLSYKAGIRVFATGGIGGVHKSWNETLDISSDLKALSEIPIIVVSA
                     GCKAILDIPATLEVLESEAVPVLGWKTNEFPAFYSQKSGKKIDKIESAKDVVNIYKTI
                     QKLYPNPTGILVANPVPEKDQIPYKEIAEHIIAAIDEAKAKRISGKELTPFLLANLAE
                     KTAGKSVQTNLALLKNNVLIGAQIAKELK"
     gene            complement(171313..171807)
                     /locus_tag="PLB65_00645"
     CDS             complement(171313..171807)
                     /locus_tag="PLB65_00645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOQ77061.1"
                     /translation="MKKLKKKLTACFSAFLLLFIAPVIAQTVASVNEEEFFTDRSLTV
                     IDSLQTQEPPGAPPGEKMIATYYSKRFHGRKTASGERYNRDAFTCAHKSLPFQTLLKI
                     TNPQNGKSVTVRVNDRGPFNRNRDIDLSYAAAKEIDMLAAGVLPVEVEIMMSSYTDSL
                     LAQH"
     gene            173210..173413
                     /locus_tag="PLB65_00650"
     CDS             173210..173413
                     /locus_tag="PLB65_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOQ77062.1"
                     /translation="MPKMTVDPDYCKGCGLCINACPRKIIRLSENINAKGYNYAECFE
                     QEKCTACKLCYITCPDVAITIEK"
     gene            173422..174501
                     /locus_tag="PLB65_00655"
     CDS             173422..174501
                     /locus_tag="PLB65_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HOQ77063.1"
                     /translation="MSKILMKGNEAIAEAAIRSGCRLYFAYPITPQSELIEYMAKMMP
                     KVNGTFIQAESEVAAINMVYGAAGSGKRVMTSSSSPGISLKMEGISYIAGAELPCVIV
                     NVQRGGPGLGDIQPAQGDYFQATKGGGHGDYRLIVLAPSSVQEFADMASEAFDLADKY
                     RNPVMILADGMLGQMMEPVEFKPAKTDAELQELANQHLSWCICPNADGDKKHHHEINS
                     LELDPQVLEKHVLDLYKKYAEIEKNEARYETYNVSDDNEVLCVAWGTASRVVKSAINE
                     VMKEGKSVGLIRPIRVWPYPYDAIKQAIGKNVKKVYVFELNSGQMVEDVKIAVEGKVP
                     VDFWGKLGGVVFTPAEIKEKLETCF"
     gene            174517..175266
                     /locus_tag="PLB65_00660"
     CDS             174517..175266
                     /locus_tag="PLB65_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOQ77064.1"
                     /translation="MEIIAKRPEALTKEPFHYCPGCMHGVAHRLIAEAIDENGLINEI
                     AGVAPVGCGVFAYKYFNFDMAEAAHGRAPAVATGIKRARPDMHVFTYQGDGDLAAIGT
                     AEIVHAANRGEKISVFFINNAIYGMTGGQMAPTTLPNMKTTTSPYGRNVEDIGYPIRV
                     CELLSTLVAPYYIERVSLLTSADIIKAKKAVEKAIRYNKENRGFTFVEFISTCPTNWG
                     YEPEKARQWAKENMLPFFKPGVFRDKGEGVE"
     gene            175266..175811
                     /locus_tag="PLB65_00665"
     CDS             175266..175811
                     /locus_tag="PLB65_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOQ77065.1"
                     /translation="MTTEMICAGFGGQGVLTIGKFIAQAAMQEGKNVSWLPSYGPEMR
                     GGTANVSTVVSTEPIASPIVSFPDVLVALNQPSIDKFAHSIRPGGVLIYNTNMCPRGC
                     QREDIIQIAVPMNEIASEIGSMIVLNMLATGIIIGKTNIIKYETLEGNLTSFLKEKNP
                     DLLAKNLAAIQRGIEIGKQYA"
     gene            175903..176700
                     /gene="pyrE"
                     /locus_tag="PLB65_00670"
     CDS             175903..176700
                     /gene="pyrE"
                     /locus_tag="PLB65_00670"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOQ77066.1"
                     /translation="MEEKVIVTVATYTNLFEAELAKSLLEDSGFHPNLLNERMMGMYA
                     SIAGDMYKIELQVPESEEQEAKELITGLEDSDYVTRILQSEGALLDGHFQLTSGKHSN
                     QYIEKIRILQNPEAAHNLCKRLAEHLVEYEFDAVVGPAYGGIALAFEVALIMKKNFLF
                     TQRKDGEMTLRSGFDLSKLKKVAIVEDILTTGGSIQEVISCLENRGLEVVAVGVLVDR
                     SGGKVQFSVPLESLFCIDIPAFNPDNCDACKQGLPLVKPGSSDKVSF"
     gene            176707..178149
                     /locus_tag="PLB65_00675"
     CDS             176707..178149
                     /locus_tag="PLB65_00675"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ77067.1"
                     /translation="MPKITIVTLLPGEIEKVIDVCKFSPINSELEIEIIYPQKDVSSH
                     QAGNLKLTKFEDVQELPVPDKSHICFFASPISCDLYRKQAKINVSSLYVHPELAILQL
                     QSDNPFKTKLSDYGLSPCVLEGDAAADLYIEQDRLIYFCDFQQFMQNKKPNFVLLSTN
                     TQFVESLISMDSAHWEHFHPELNYRNLCVGYNNPHRLLLHSQKQLLRLIRIPDSIYDL
                     GGIRYSQQDDSTAYSFFATHYDQYMAHVDYESWVNYVLSWFKQYSSLQLNKILELACG
                     TANFSEQLVNRGYQVYACDNSIDMLHMAAKKAVKPILYYASLTDPIPGRNYQLIICMF
                     DSINYLTQAKQITKTLNEVSLALENGGLFVFDISTLENSLDNFSDVSELYHYSDGLML
                     HQAWFESVRLMQKSALHFFKKDTIGYCYQYEQHLQRVYFTNELIELIHNSPLKLRAIH
                     STDTKTNFYPKHLNSIDEKYSRLFFILEKI"
     gene            178152..178751
                     /locus_tag="PLB65_00680"
     CDS             178152..178751
                     /locus_tag="PLB65_00680"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOQ77068.1"
                     /translation="MTNLYQNDIKHLTENGVFAGIDEAGRGALAGPVVIAAVVLDYNK
                     IIEGLNDSKQLSPKKREILFQQIVELALDYSIVEIGPDYIDKHNILQATLLGFKKAFL
                     QLKSKPEHCLIDGKDIHKDLKPYAKAIIKGDQLYAAIAAASILAKVHRDKLMLAYDEI
                     YPEYGFASHKGYGTAFHSKRIHSFGLSPIHRKSFKVPEV"
     gene            complement(179015..180169)
                     /locus_tag="PLB65_00685"
     CDS             complement(179015..180169)
                     /locus_tag="PLB65_00685"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ77069.1"
                     /translation="MNKIYDVIVIGAGSTGLPTAYNLAKAGLKVLVIEAEHSPGQGNN
                     KKAIGGVRATHSDFGKINVCLRSIEILKNWSEEMGEDIGWLSNGYSYPAYNETDAKTL
                     KDLMQVQLGFGLNIKWLNPDEYSDIVPGIWKEGLQGSTYSPEDGSCSSLLVSAAYFEH
                     CKEEGVDFHFQEHITGFNIVSSKITKVITDKGIYSSAKVVNAAGSYAREVGQMANLDL
                     PVFPENHEAGITEPVERFFGPMVIDMRVAPGSSNFYFYQNSEGQVVFCITPEPPISGI
                     DTRSTSLFLPMCCKRMLTIYPRLRYLKVRRTWRGQYPMTPDGFPIVGIMDEAENLINA
                     VGMCGQGFMLGPGMGELVTRICLESTTEKDKEVLKSFDPYRQFTGQEAFK"
     gene            complement(180166..182244)
                     /locus_tag="PLB65_00690"
     CDS             complement(180166..182244)
                     /locus_tag="PLB65_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ77070.1"
                     /translation="MADSNRIAIHPILPVPDKKEIYFYWNGQKMQAKCGETIASALIA
                     NGISVFGHHHKDGSAQGIFCANGQCSQCSVIANGLAVKSCMTAVSENMIVQSLEGLPV
                     LPAVDNPLSFEPLEQIDTDVLIIGGGPAGLSAAIELGKQNIPTLVIDDKNALGGKLVL
                     QTHKFFGSQADSSAGTRGIDIGKNLAAEIATYSSVDIWLNSTAVFVFSDKKVGVLKDG
                     VYKIVQPKRILNAAGAREKFLRFPGNNLAGIYGAGAFQTLVNRDLVRPTQRLFIIGGG
                     NVGLIAGYHSLQAGIDVVGLAEAMPVCGGYKVHSDKLKRFGVPIYTSHTILCANGDDT
                     VESITIAELDKNFQPVKGTEKTFTCDTILIAVGLNPLSEFTLEAEAAGLPIDAAGDAL
                     EIAEASSAMFNGKIAGVKIASILKGEKKNPIPDDWFIKAEELKSAPGKTNNYNTPEKE
                     EGVFPILHCLQEIPCNPCTTVCPTNSIHTEDGSLTAVPVYTGSCIGCGKCLLICPGLA
                     ITLVDYRKDKENPTISIVYEVSNYPVKVGDNKILNDINAKELGKYAITAVQDFPKQHT
                     QIVKFKVPKAIAKKVAGFQIQDANVSAPLTKPVLLEKTADEAMVCLCERVTAGEVRAL
                     IRKGITDMNLIKSITRAGMGPCGSKTCEVLIKGLLREEGIPEKEIAPITKRPLFIEIP
                     LEKFPNGSAK"
     gene            complement(182528..183616)
                     /gene="lptG"
                     /locus_tag="PLB65_00695"
     CDS             complement(182528..183616)
                     /gene="lptG"
                     /locus_tag="PLB65_00695"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOQ77071.1"
                     /translation="MKILDKYIIREFLRTYLIIFLSFVAIFVVIDVIDNLPRLIRNGA
                     TTQQATIYYLMRLPYLLVLTSPVTVLLTGLFMMSALSKHNESIAIRAAGISIKRAMLP
                     LFGIGLIISIAVALFGEYVVPWAETTKNYVYNVQIKGQQPDDQMLKARIHYQGKANDF
                     YYFGFFDGYKNNLKIIDLTRIDYKTHQISEHITASTADWNGSRWVLRDCEIRHFDNGK
                     QVFMAYYPQTEIVNLDVQPQDFIRITQKTLSLNFWQLREYIDRMKKLGDDPAREIVDL
                     HMKISFPLTNLIVIFFFIPIATSNIRSKGRGWIIMLGLVVCFVYLIVVRVCQSLGYNS
                     IINPVWAAWFPNLFFTILGLGFIYKAEI"
     gene            complement(183619..184947)
                     /locus_tag="PLB65_00700"
     CDS             complement(183619..184947)
                     /locus_tag="PLB65_00700"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HOQ77072.1"
                     /translation="MKILKRYLLKEHIAPFFIALMVVTFVLLSDRIIDLLNLIIEKKL
                     PWQIIFELFGLSLPYMLALSIPMAVLVATILAFGRMSVDREIIAIKSSGINIYSLIGP
                     LVIVALLLTGLMVYFNHWFLPETNHKLKNLTLKIAYYKPMTIIKEKEFTNFMDYTIFA
                     NEATDSLLTDVLIYDRSQTHFPRTVFAKTGNVIQKDNGNSLQIILNNGEMHERNEKEP
                     GKYQKTTFTNYVINVRNLGSNVDMFETGYRSDREMNYGQLVAAIKGHKEELTIKQQEI
                     VELNKRIELLKKSPPSFTRNMELQRLHSMQKMTSDRVEELKELISSLQVEYHKKFSIS
                     FAIIIFILIGIPLGLMTRTSGIGMSFSVSSVIFLIYYIALNAGEQLADKGNMPPFLSM
                     WLSNIAFLIIAIFLIISSIKEKKLIDMPTLMWRIKHYRNRKAPVPDEIVH"
     gene            complement(184944..186947)
                     /gene="ligA"
                     /locus_tag="PLB65_00705"
     CDS             complement(184944..186947)
                     /gene="ligA"
                     /locus_tag="PLB65_00705"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HOQ77073.1"
                     /translation="MNKEEIKAEIEELRAEIEKHNRLYYVLAKPEISDYEYDMLVKHL
                     QDLEAQYSEETIEESPTRKVGSDLAPGAKTIPHKVRMTSLENAYSLEEVLSFMNRTNN
                     DLGYNSEYCAELKIDGFSINLYYENGEFIYATTRGDGLEGEDVTENFRLIPEVPFTID
                     YKRPVEIRGEIYIPIQDFLALNETRRENELKTFANPRNAAAGSIKLKNSEEVKSRHLQ
                     ALFYALGYYESPLISKNEVVDKQTRLLDFLTELGFKTSKRALCNSYKEIEEFCLQVEQ
                     ERYTLPYDIDGIVIKVNNLEDQKKLGWTAKSPKWAIAYKFKPEEKETILESVSFQVGR
                     TGAVTPVANLKPVYISGSTVSHSTLHNADEIKRLDLHYGDTVLVVKSGEIIPKILSVN
                     ISKRPQNAKPVEFPVSCPVCYTPLERDEDGSIIYCPNANCPAQVQRKLEHFASREAMD
                     IYGLGSSLIARLIETGQIKSIPDIYHLDYDKIASMDRMGKKSAENLRKAIEASKTKNF
                     DRVLFALGIRHIGLITARTLAQHFKTIDALIAATDEDLLGVPDIGSIIAHSIIGFFSQ
                     KSNLELIDSLKAVGLQFAYTSSQRSNTLANKTFLITGTLSNYGRKEMESLIMEHGGKI
                     LSGVSKQLNYLIVGENPGSKLDKAHQLGTVKIINELELLDMLK"
     gene            complement(186944..187735)
                     /locus_tag="PLB65_00710"
     CDS             complement(186944..187735)
                     /locus_tag="PLB65_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HOQ77074.1"
                     /translation="MKVSVLQFEPRLLDKAYNLMRITDLLSSLKTDLVVMPELCTSGY
                     VFSTKEEVYSIAEVSGKGEAFLALSNIATKNNCSIVYGYPEVDGDKLYNSSMLIMPDG
                     DYHNYRKTHLFNREKLFFSPGDTGFQVFTTPAGVKIGMMICFDWQFPEAARTLALSGA
                     QIICHPTNLVLPWCQAAMKIRSLENRVFTITSNRIGTETNAGVTISFTGASQVLNTKG
                     ELLANLPVNKEGIATAEINPLEANDKTITERNDAFKDRRPELYRL"
     gene            complement(187732..188946)
                     /gene="hydF"
                     /locus_tag="PLB65_00715"
     CDS             complement(187732..188946)
                     /gene="hydF"
                     /locus_tag="PLB65_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HOQ77075.1"
                     /translation="MSSTPRGERLIIAIIGKRNAGKSSLINALVGQEIAIVSEIPGTT
                     TDPVDKHYELLPLGPVTFYDTAGVDDTGELGQKRVQATQKILYRADIAIFVSDGRPFT
                     KAELTMMDKIIGMELPILVVFNKSDIIEANPENMDLCLKKSLPYVSVSCITKDGIREC
                     KDKLINLAPQVLKDNRIIAADLVNPQSNIILVTPIDSSAPKGRLILPQVQTLRDLLDR
                     NCVVTVVKETELKSALENIKEPPSLVITDSQVIDMVAKIVPEEIPLTTFSILFARYKG
                     ELDILLEGINRLKSLKDGDKILIAEACSHHIQKDDIGRVKIPNWMRSFSSKTLTFEVY
                     AGHDFPDNLEEYAVCVHCGGCMITSMEMNRRIMECHRRGVPITNYGLTISYLKGVLDR
                     TIQPLMKEKKEQ"
     gene            complement(189180..189830)
                     /gene="coaE"
                     /locus_tag="PLB65_00720"
     CDS             complement(189180..189830)
                     /gene="coaE"
                     /locus_tag="PLB65_00720"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424716.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOQ77076.1"
                     /translation="MQLNNRPLLIGITGNIGSGKSSFCNYLAANGLRIISADVLANQH
                     LEDKDIQSALVTRYSPDILTTSVRDKNVLTINRKLLADKVFISETETNYLNSLIHPLV
                     LQDFQQIAEQSQEKVLCFEVPLLFEANLQDCFDYLILISASKEKRLDRLEKKGEEKSK
                     AEKRIMHQIPDINKRNLVDLVIENNNDIDALHKAADNFANHISEIKLKEVRPFILV"
     gene            complement(189817..190563)
                     /gene="cdaA"
                     /locus_tag="PLB65_00725"
     CDS             complement(189817..190563)
                     /gene="cdaA"
                     /locus_tag="PLB65_00725"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HOQ77077.1"
                     /translation="MEFLIPRYQDIVDILFIAFLIYQALLIVRKSGGYQVLWGLLFVL
                     VLYIVAIIFNLKMVGSLLNAIRNYWFIAVVILFQPELRSILSHLNLPRELSKAFSKQE
                     KSSLYTPIIDAVSSMSFRKIGALIVLENKRKLNEYIYSGEPIDAAVSMRLILTIFNPK
                     SVLHDGAIIIRDQRIMAAKVVLPLSRKDEYVRKFGTRHLAGIGITEISDAIAIIVSEQ
                     TGQISVANAGKIQTNVAFEELMQILTDATK"
     gene            complement(190564..191397)
                     /gene="folP"
                     /locus_tag="PLB65_00730"
     CDS             complement(190564..191397)
                     /gene="folP"
                     /locus_tag="PLB65_00730"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HOQ77078.1"
                     /translation="MNKNYYPEHIPVIMGILNVTEDSFSDGEQFLDRDSAIKHSIEMI
                     AQGAEIIDIGAESTRPGSLPVNSETQLKRIVPIITTIKEKYPDQILSVDTQNTAVAKK
                     SIELGVTIINDISALRTDSEMAELLADNPQIKVVLMHMQGIPRTMQLNPFYTDVIAEI
                     NDFFQERIDYCLSKGILLENIILDPGIGFGKTLEHNLTILANLKSFKQFGLPVLLGAS
                     RKSFIDKISPSLPSQRIGGCLAAAFAAACGEMDIIRVHDVLAHKQFFDVLIAIAKAGK
                     N"
     gene            complement(191390..192109)
                     /locus_tag="PLB65_00735"
     CDS             complement(191390..192109)
                     /locus_tag="PLB65_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77079.1"
                     /translation="MRRFNPFKLFNGYFISVVFHLLLFLFFAFYFLKPYTPEKWYSFE
                     WESSQENKVKKTSSLRIEDLIGGKNAAGSAGISKSQESSGNMSLNLNKLAPERAGESE
                     SVITMPLNVPNQEENKANIPSSFTRNRGAHSLKEIPGTFQKTNPNFSATLEEGGGEAY
                     IISQSKPQIIPTEEGEVYIEFKLMRNGAIDMNSVNILSFTNAAYVESIRKVMPSWKFG
                     FKGVYNPKTTYRLLCRFVINE"
     gene            complement(192112..192513)
                     /locus_tag="PLB65_00740"
     CDS             complement(192112..192513)
                     /locus_tag="PLB65_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOQ77080.1"
                     /translation="MKLRVSKQRISTTMLISMTDVIFLLLLFLLIGSNFVSQTGLPIR
                     LPGSSTSVRQSYETLLITYYNDGHLYFMDKPITIQQLKDILQQKYQTPEQVVRIAVEK
                     QVSVQKLIDLMDIVREIGYEHIFIATTVEKG"
     gene            complement(192510..193136)
                     /locus_tag="PLB65_00745"
     CDS             complement(192510..193136)
                     /locus_tag="PLB65_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOQ77081.1"
                     /translation="MSILDLIVKGGFLMYVLTVISVISIGIIIEKYRQFRKVRIANNK
                     LIESLKSQDKLENIRALINLHQNECPMGIMLYKLFNSPSDDLDLINQSMEATANLELH
                     KLERGLGWLSTFAAIAPLIGFLGTVLGMVNVFMKIQAANQSGVDISMLAGGIWVALLT
                     TVGGLIVGIFTIIFYNDLVQKLENIVKDMQDNAIEHIIKYKKIMQGKL"
     gene            complement(193233..194318)
                     /gene="serC"
                     /locus_tag="PLB65_00750"
     CDS             complement(193233..194318)
                     /gene="serC"
                     /locus_tag="PLB65_00750"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HOQ77082.1"
                     /translation="MERVHNFNAGPAVLPEEVLREAQSDLFNYKGMGLSVMEMSHRSK
                     EYEAIINEARNAAKRIYGLGDDWDVLFLHGGASLQFLMVPLNFIPEGKVANYIHTGVW
                     SKKAMAEVKKLNKPLHIAASSEDKSFSYIPKNYQLSDNPAYLHITTNNTIYGTEWKTD
                     PDVPEGIPLIADMSSNFMSKPIDINKYSLIYAGAQKNVGPAGCAVVLVKKDFLATAAA
                     NLPSMLDYQIHAKNGSMYNTPPCFTIYMVGLVMKWIENYGGLAMIEQNNKAKAKYIYD
                     AIDASNGFYRGTVIPEYRSLMNITFRLPNEELEALFISEAKKNGMIGLKGHRDVGGCR
                     ASCYNALPLNAAYDLAEFMKNFQKQNG"
     gene            194460..195053
                     /locus_tag="PLB65_00755"
     CDS             194460..195053
                     /locus_tag="PLB65_00755"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOQ77083.1"
                     /translation="MIHELLHNKKVILASASPRRKELFALLGINAYIIPAEIAEPING
                     EEPQVQAMQNALRKAEIVKNKVDKDAVVIAADTLVAIDNHILGKPLNQKEASRYLRLL
                     SGNTHSVYTGICIFYNEIANINYEQTLVHFAELTELEIKSYIETGEPMDKAGAYGIQG
                     YGAQFITAVDGCYYNVMGFPVRKFYEMLNAILEKEEK"
     gene            195050..196513
                     /locus_tag="PLB65_00760"
     CDS             195050..196513
                     /locus_tag="PLB65_00760"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOQ77084.1"
                     /translation="MNILANLYLWIKGKFTEEPKSLLVHNTSISEILPYPVPEQYKNI
                     SMKDLNGSYAYPGFIDTHTHSFEGGLYSLGVDLSTAKNIEDILALLSAANSKKTKDEF
                     IFAWQMDETLLKERRFPTLTELNSAVPDKPLILRRIDGHSCMLNSYARNMVTGLKTKE
                     EILRGGENDYAVHFFHKRLNDEAILNAYRAAANIGLQAGFTSLHTMIGDAENSINHYE
                     LIRDNLNKFPLRFILYPQSFNIKAALEAGANRIGGCILADGSIGSETAAISEPYLNSN
                     SWGVLYQSDEFWQKFITEASQHRLQVAIHCIGDRAIRQINNVYKSLASTADLRHQLIH
                     CEITPDNLIEEIATSGAVPVMQPNFDYLWGGENGFYAKKIGIKRSRIMNRFATLLKSG
                     ITITGGSDWYITSLNAAHSLRAAINHHNPLERLSTAQAIDIYTKNAAWLNSEEDTYGK
                     IKIGYKADLSIYSHPIENETTELYYLIRDGEIKYAAI"
     gene            196500..197081
                     /locus_tag="PLB65_00765"
     CDS             196500..197081
                     /locus_tag="PLB65_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77085.1"
                     /translation="MQLSDNDLIKVDIKQVIKQELLFHPELQLIDLYKLVFQAYMGPG
                     HLINNIDEAANYIITEMLLHSEPYLPIKQDIGNGKGFFRVSLDLLMPEKELKKEQAIV
                     EIYRKSYILADLMKQSVYDNNHNLNITDIWNATVPQIKELIDFDLAEWKEVDLIAESK
                     AIPRHSQIYKTLYNPKYRVLKYNFYDNIDKLVK"
     gene            197101..198534
                     /gene="gltX"
                     /locus_tag="PLB65_00770"
     CDS             197101..198534
                     /gene="gltX"
                     /locus_tag="PLB65_00770"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOQ77086.1"
                     /translation="MNEIRVRFAPSPTGYLHIGGLRTALYNYLFARKEKGTFILRIED
                     TDKSRQVPGAIENLINTLNALGLDYDEGPGRESKFAPYIQSERLSLYKEHAQYLLDSG
                     FAYPCFCSPETLTKMREEQQSKGEFVKYDRRCLSLTKSEIEQKIAAGEKYVIRLKMPD
                     NRKFQFNDLIRGEVEMDSNQSDDQVLLKSDGFPTYHLAAVVDDHLMQISHIIRGEEWL
                     SSTPKHIYLYECLGWETPKWVHLPLILNADRTKLSKRMNDVSVDSYIAKGYLKEALIN
                     FVALLGWHPSDDRELFTLDELCNAFSLERVNKAGAIFDLTKLDWMNGQYLHSLPVEDI
                     AMRCKHLFLEKGYDLSDENKYQLAIAVARERCTLLNEIVDYCKMFYQPEELKNDDIKT
                     LLAPEAQKVLAWFATNLPILSAWDTESIQNLVNSGIAELGIKGKKFYTPLRLALIHQQ
                     HGPELPSTFIILGKEESIKRLQQYLPS"
     gene            199088..199405
                     /locus_tag="PLB65_00775"
     CDS             199088..199405
                     /locus_tag="PLB65_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77087.1"
                     /translation="MAKYGRLLIIEISLFFLGACQEESLLGPLSESFDPLSIQEDRIP
                     LAGGEYLYQQNIVITNTEDNDFFAYKLITYQGELPVSYMNQTETANALQNLFSNMINN
                     EPL"
     gene            199416..199790
                     /locus_tag="PLB65_00780"
     CDS             199416..199790
                     /locus_tag="PLB65_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77088.1"
                     /translation="MKHYIILILLLSIVLLFGQTKPSDAKSVNYETHNSGLLSLNVYN
                     IGSLSNLHNPYDGMKLMSQSGQWISAKKYRRNNLNQLLYWLSPIPGPGNDQMVTEMDP
                     LWNPNLLVVTDTLSTVAYDGDC"
     gene            complement(199824..201827)
                     /gene="uvrB"
                     /locus_tag="PLB65_00785"
     CDS             complement(199824..201827)
                     /gene="uvrB"
                     /locus_tag="PLB65_00785"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HOQ77089.1"
                     /translation="MQRFKLVSDYIPSGDQPEAIKSLVKGIKEDKKYQVLLGVTGSGK
                     TFTIANVIEQINRPTLVLSHNKTLAAQLYGELKQLFPENAVEYFISYYDYYQPEAYIP
                     GKDIYIEKDSSINEQIEKLRLRATMSLMERRDVIIVSSVSCIYGLGVPEEYREALISI
                     SRGMKMDREELIKKLVAAHYSRNDIAFERGAFRVRGDTVDIFPAYLEHCLRVEFFGDE
                     IYRLEKLHPISCNSLGEVENYDVYPAIHFLMNEDKLHKAIINIENEMNERIAWFMQNQ
                     KYLEAERLKQRTAFDLEMLRELGYCSGIENYTRHLTGAKPGEPPNCLLDYFPEDFLFV
                     IDESHVTIPQVHGMFGGDYTRKKNLVEYGFRLPSAFDNRPLRFEEFENYMRNVIFVSA
                     TPANYELELTQGEIVEQVIRPTGLLDPEIEIKPVKHQVDDLIGQIKQRTVKSQKVLVM
                     ALTKRMSEDLSTYLINAGIKARYMHSDIDSIERAKIIRDFRLGVFDVIVGVNLLREGL
                     DLPEVSLVAILDADKTGFLRSTRSLIQISGRAARNVDGKVIFYADEITDAIKQTITET
                     NRRRVKQLAYNKAHNITPQTIYKTIEQIMQSTAIAEGYDKISKPITDEKPSEKDFLEY
                     LDLDNKEKIIELLTKEMNKAAANLEFEHAAELRDRIWELQQNL"
     gene            201949..202272
                     /locus_tag="PLB65_00790"
     CDS             201949..202272
                     /locus_tag="PLB65_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77090.1"
                     /translation="MLNYEDLQINYENLLKLGYAVVDVRYKDYDVCPDIPKLVIARVD
                     SDRDDFYQDMLKLYTGIEFKPNEMFEIWTDILSHKIKMSIVLHRDIAIKVAALDYIET
                     VYPSK"
     gene            202269..202682
                     /locus_tag="PLB65_00795"
     CDS             202269..202682
                     /locus_tag="PLB65_00795"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA];
                     GO_process: GO:0006183 - GTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006228 - UTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOQ77091.1"
                     /translation="MKEQSLLLIKPNAVRNKHIGHIVTILEENDFHFCALKIFRFDRQ
                     LGELFYAEHKGRAFFENLLSFMISDDTVAIIVEKDNAVQELREIIGDVDPEKRKPGTI
                     RALYAEGITENAVHASDSITSAIREIGIIFDKQTN"
     gene            complement(202925..203800)
                     /locus_tag="PLB65_00800"
     CDS             complement(202925..203800)
                     /locus_tag="PLB65_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HOQ77092.1"
                     /translation="MNLSDINNLIQRATVLAKTNWLQAVYLLQEGLKEEPDNYSLIIN
                     LGDIYLERQIYEKALSYYIKAISLHPDSPQLLFLIGTCYFSLGEYRIAISYFNRISNP
                     PPEVLYNSALAYAFLGCYQESIDVINKILEVMDDNPFIYFLLIEQYVRMQNYDQAYQT
                     IINAEKKFGKHRQLLLLSALVYGKKGIWLKSYHSFVEYESLGHITNPDHLIAYANAAT
                     NIGLIDRAIEILRQAQEINPYISVVYEELIRLLLQKNNYKEARKVLSLAKKYISQFSP
                     VLYLFKERLDNHPPE"
     gene            complement(203802..206969)
                     /locus_tag="PLB65_00805"
     CDS             complement(203802..206969)
                     /locus_tag="PLB65_00805"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOQ77093.1"
                     /translation="MKTIKNTLKRIFSIVMLLSFSYTLIALETPFAGDPAISPDGKTV
                     CFVYCDDLWLVPFEGGIPRRITNTTCKEWNPQFSPDGKMIAFSSDRDGQSMIYLMPTE
                     GGISKPVTYESLGLSEWFSDSEHLLCTKYTPRWGISFYKVPIDGSRPVMIAEVATRFA
                     TLSPDNKQIVFQKGGYSRREAYRGSANGELWLLNIETKKYTKLTNTELSEMYPRFSQT
                     GALYYCASDGKNLQINKVNKLSFRKPEKITNLSPFSARDINLARDNERLVFEYFDTIY
                     KYDPTIKTGEKVSPLYIDLSEDEWQDNIVRSYSNNNVGDYTVSINELLVGFTHNYDCF
                     FAPVKGGEVKQISYDHGSISSMQFLDNRRMVISKLDNGIVKLFVATADSIITLSPIEW
                     FGADSLCVSDLGKDKNGRWYFKYADYYSHQKVAIADSGLINIRPVKTPWAVTTNFAFS
                     EDGNYALYGCMRDDNYVKELYLYDVKADTSKRILADDGWLDNLVWMPDNRSILLTRNQ
                     DIYRLDLVPRDEFELDKDPWEEILNQEKSKPDSTNEEMYYDENGYLVLDIVAEENKTE
                     TNNKLEIVWEGIEKRFYPIIIAKDNYNFVLDTISDSTFYYVEDANQPNKNNYLKKANI
                     YGKNQKEVFNFGSTANAYCKVNSTVYYLVQGELKSYNLATSSRKDIKVNYDYQYDKAL
                     LNQRVFEEAWSAFGNNFYDPDMHNVSWKELFRLYRPYVDKARDIYDVATIIEEMIGDV
                     NASHTGFYPRSEYSPFRNPVAWLGAEFDYNIILEEGIVLSRVYPNSRLSNFYHLKDGC
                     ILTNVDGVKITSKTSLDSLLAGKIGKKIKLTVVQDGVDIEAVITGLSYSQMYELRYNY
                     QIAQNRKLVDDLSHGKLGYIHIPAMGNDDWKQFYREFLVDNYDKDALVIDVRGNSGGH
                     IHDQIITLLQKKKYAYTATRRYSRILRSEPRSIWDKPSIVLVNEESFSDGEIFPMVYQ
                     ELKLGKVVGTPSSGAVIGTWHYYLQDGSSMRMPGTGWYKLDGTNMEGTGVMPDIIILN
                     LPEDIIAERDPQLVRAVKELLKELK"
     gene            complement(206969..208261)
                     /locus_tag="PLB65_00810"
     CDS             complement(206969..208261)
                     /locus_tag="PLB65_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FapA family protein"
                     /protein_id="HOQ77094.1"
                     /translation="MDKTYTNKEGNLILEIRDEPLSAWLTIKKTGKLIDEYAIIALLE
                     EAGIKSGFEEAVEYAQIHSLEKEFDIPFPVAMCKKDENKTILRYKFNPDILTAPEMEI
                     SYQTLAKLKVFKAGDTVAEYSHNIFEQKGSIYNIFGELIDASSVDPEQAIALAGENVA
                     YDVQNCTYYLLKDGYPYVDEEGHISLLDSITLKASELPSDTIISCPIDLIIEGDLTYA
                     NLHCDANLRIEGEVHSCSIECKKDMLVTGDINASSAKGIIVWGNLECSSIHNSYIVCL
                     NNIHFTNKLENSTVVCDGEIIGAPDRSEICGGLTQAGESITAFQAGSYNNTITEIEIA
                     ISPFYRNLLLILTKDLIRLKENKDINATAIVDLENRIQNYEILLDDKLNQFLNREQKD
                     TKRISIQDISRPPIIYRILKHSYEINKREKKIEFIEKE"
     gene            complement(208254..208943)
                     /locus_tag="PLB65_00815"
     CDS             complement(208254..208943)
                     /locus_tag="PLB65_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HOQ77095.1"
                     /translation="MKIFYHLGKRDTDYRSLMHLQKDFILEGTKIPISRVIIAEQTHS
                     KQVHICREIDGGAGFMNKPQIPVVDGLITKIPYQYLLIRTADCYPVILMDNRINVIAA
                     LHCGREGTRKNIVGEAIRIMNSQMYCQLTDIIAIVGAGICHNHYEVSQELYNEFGKSI
                     VSQGLEPVLTKNRHIDLRKTLNAQLLKAGIPEINIEHINDCTFENSCYHSYRREKTNN
                     RQINIVGIIHG"
     gene            complement(208940..210250)
                     /locus_tag="PLB65_00820"
     CDS             complement(208940..210250)
                     /locus_tag="PLB65_00820"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ77096.1"
                     /translation="MIISKLKEIFVSLTIRNYRLFFMGQGISLIGTWIQRTTMGWFVY
                     RLTGSAFLLGLVSFLSMVPSVFVSPFAGAWADRVNRHHIMIYTQIAFCLQTGILAILV
                     LTGVINKQVQYPIIILSLLQGIIDAIDAPIRQNFVMDLVSSRRLLPNAIATNSAMFNG
                     ARLIGPAIGGFLIMMFSEGVCFAINAISYIPVIISLCFIRISYPPPSPQKEPTLKKIV
                     SGWKYAWENMPIRFLISNLAIYTIFGMSFSTLMPIFAKDILQGTSATQGLLMSAMGVG
                     ALAGSFFLASRKNIRGMPTRLVYLCLVYSISLILFSLSGSLPLSMFYMVFIGLTGMMT
                     MATTNTLIQSIASIEMRGRVIALYTMASASTAPLGSILMGSLSSQFGARYTLIICASV
                     FLLWSLYSLKLAPKFIKGVLRMLVISNNTDVYRSKILAPEINIQ"
     gene            complement(210253..211614)
                     /gene="hslU"
                     /locus_tag="PLB65_00825"
     CDS             complement(210253..211614)
                     /gene="hslU"
                     /locus_tag="PLB65_00825"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOQ77097.1"
                     /translation="MLNYVHLTPKRIVEELDKYIIGQDQAKRCVAIALRNRWRRQQIS
                     GDIKGEIIPNNIILIGPTGVGKTEIARRISRLTGAPFVKVEASKFTEVGYVGRDVESM
                     VRELMNYAVSQIREMMIEEVRPEAEKNATEMILDILLPRSKKMKQNRVSDNAEYDRYL
                     RSREKMRKKLLSGSLDDKEIEIEPELDTTPNLEILPGPGLELLDLDFTEMLTNIFQGK
                     GASRTIKTTVHTAWKRYIETEINRLISKDKLIETAKNSVEENGIIFIDEIDKIANIDK
                     QGGLDVSRSGVQRDLLPIVEGSKVPTKYGMIDTSHILFIAAGAFHSAKPSDLIPELQG
                     RFPIRVELNSLTKEDFERILIEPENSLTKQYQEMFRSEGVELTFMLSAIREIARFAAL
                     ANEKMEDIGARRLHTVMNTLLDDFLFEMPSETLKSVKITKKMVTEYLNPVIESEDLSR
                     FIL"
     gene            complement(211618..212148)
                     /gene="hslV"
                     /locus_tag="PLB65_00830"
     CDS             complement(211618..212148)
                     /gene="hslV"
                     /locus_tag="PLB65_00830"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOQ77098.1"
                     /translation="MIMHGTTIIGIRRNGKTAICGDGQVTMGEAIVKGNAQKIRRIFD
                     GKVIVGFAGATADAFSLLERFEDKLKSNKGNLRKAAIDLARDWRQDRILRRLEAMLIA
                     GDKDTLLLINGVGDVLEPDDNIIAIGSGGNYALAAARALARKTKLGAGEIAVEAINIA
                     SEICIYTNSNYIVEEV"
     gene            complement(212151..214355)
                     /gene="ftsH"
                     /locus_tag="PLB65_00835"
     CDS             complement(212151..214355)
                     /gene="ftsH"
                     /locus_tag="PLB65_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOQ77099.1"
                     /translation="MMKYILILFCIIALGFLNATTADTLTVPQKTLPEAGNAFMQFST
                     LMNWFILALLLISIISIIRSIIVRNRNIKNKDKPNPDFRNPQPPKNVPPKKSAFSLPM
                     IIMLILMVIIIFHIFSTSGTSITKESYTNFMARVANGQITQVQFSEKDIFYTDIANKK
                     YTTTLPFENPQLVDSLVAMGIKVSATKPSRWASIISYLLPFLLLIIFWAFFLRGMNAQ
                     NAKAFSFGKSKARLYEASKTGVTFKDVAGVDEAKEELQEIVEFLKDPKKFQRLGGRIP
                     RGVLLIGRPGTGKTLLAKAVSGEAGVPFFSISGSDFVEMFVGVGAARVRDLFEQAKKN
                     APCITFIDEIDAVGRHRGSGLGGGHDEREQTLNQLLVEMDGFEPNEAVIIIAATNRPD
                     ILDPALLRPGRFDRRVTVDLPDIKGRTEILKVHTAKVPLANDVHLEIISRGTPGFSGA
                     DLANLVNEAALIAASKNKTQIQMDDFEEAKDKLILGKEKKSRVIPEEDKRLTAYHEIG
                     HVLTSVFLDKVEAVHKVSIIPRGFTGGATHYLLSDKTNYSKSYLLQLLVTLLGGRAAE
                     EVIFTEFTTGSGNDLERSTDIAKKMVCSWGMSDKIGPITIGKEQGEVFLGKELVSRDI
                     YSDETSQMVDREIREIINNAYSQAISILNEHKELMEIMANELFDKETLGTDEIFKLIL
                     DNCRDDESEIVQNKYKKALELRFEPGEKARVSEPETLEDKTEDTDTKLEETS"
     gene            complement(214352..214897)
                     /gene="hpt"
                     /locus_tag="PLB65_00840"
     CDS             complement(214352..214897)
                     /gene="hpt"
                     /locus_tag="PLB65_00840"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HOQ77100.1"
                     /translation="MNKMNCDISAVLFDEHKIQTRIRELGLEISNSYKDSTPVMVGIL
                     KGGFIFLADLVRSITIPVEVDFLAISSYGNDANSSGIVKIRKDIDIDLSNRDVIIVED
                     IVDTGLSLEYIKDYIWQHKPASLKTCVLLDKPDAHKIDVCFEYIGFSIKNEFVVGYGL
                     DFAERYRNLPYIGILKEELYK"
     gene            complement(214894..216327)
                     /gene="tilS"
                     /locus_tag="PLB65_00845"
     CDS             complement(214894..216327)
                     /gene="tilS"
                     /locus_tag="PLB65_00845"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOQ77101.1"
                     /translation="MEIAQALQRLEKNINENELIKIGDKLLLGCSGGADSNAMLYLFS
                     QLRVTYNLTLLAVHVNHQIRGAESDADQESVKELCSNLNIPLIIHKVKVPKTGNLENH
                     ARKLRFSVFAKVLDSYKFDKILLAHQREDQAETVLLNLFRGSGITGMAGIKAISGKIV
                     HPMLCFSRKELEDILSKVDITWRTDSSNLDNKFRRNLLRNELIPRLEREFNPMLSSHL
                     SFVAELFAQADKILKQRSESQYKRICLDIQPERTVLSCPLLIKLSKIERYYLLRNVFI
                     KVCDDDNDFFAVHYQAIDNILATKGSKCIYLPKNIVVIKQYEELIIQVGDIKKNTEPE
                     PMIIDTDRTMAVYGNYRFSFKYLKVQPKDHKLADGQEQIIIDADKVNFPFMIRFRKPG
                     DRFMPFGMTQYKSLKEFFIDEKVPKYERDLIPILEDGEKLFWIVGCRLDDRVKCDETT
                     SRYLQIVANPIASKSRRAIHKKEKRKG"
     gene            216917..218170
                     /gene="hutI"
                     /locus_tag="PLB65_00850"
     CDS             216917..218170
                     /gene="hutI"
                     /locus_tag="PLB65_00850"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HOQ77102.1"
                     /translation="MQADLIIINISKLVTLAHNGRPRLGKEMEETATILNKALAVKDG
                     KILEINDSKSIEEKYRNVECIDANGQIATPGFVDCHTHPIFVHTREDEFAMRIQGKTY
                     VEISKAGGGILNTIQTTRDADEDLLFELAKRRILKMIQQGTTTLEAKSGYGLDTENEL
                     KQLRVIRRLQEELLIDIVPTFMGAHEFPPEYRNNHSGYVELLCKEMIPAVAEQGIAEF
                     CDVFTEAHTFNIEESRKILSCAAEYGLKLKMHSDEIESIGGAELASEMKCISADHLGS
                     ASETGIKALQKAGVVPVLLPATLFSLQSKKYANAKLMMELNLPVAIATDFNPGSCNCD
                     SMSLTMSLACLQMGMTPAASLCAATLNAAFAIDRGKIIGSLETGKQADIVLWDIPDLN
                     FIPYHLGSSYITSVLKNGKIVYKAT"
     gene            218171..220198
                     /locus_tag="PLB65_00855"
     CDS             218171..220198
                     /locus_tag="PLB65_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HOQ77103.1"
                     /translation="MADTSLNSYQIIKTVNSSLRFSLYQAKQIETGNVVLIKTPDSQR
                     TNDIGLKHDLVNEANSHLKLNHPFIRTVYETREEKGTAYLIGEYIEGRSLSAYLKNNP
                     NSFTLEESLSRLQELLEAISYAHKMGCIHLNLNPYNILVDKENHLHIIGFGKNQDAYK
                     TAYEKDETYHPLLYIAPELYSAGIALPQSDLYSFAVIAYHILCGVIPWRVDNQLIPEK
                     QKQQSLCRAVIMPEILNKQVPDWLFAALLQCLKLDPHLRFSNAEELLELFTKRDNWVP
                     EQEAEIIKEVEKVETIVDIEKCEAIEKIEQIEDVQLVDDLPPTNLALNVDSQQKINTD
                     ISHLYGKLDVTIPEEIPQSDSVKTISDTTPKDTPKYSFTTSAPVETIETRKLKKTFKV
                     LIWMSAIVLLFIIIKYVAFGNRPKFTSVADSTQVEIPEGPLNKNLPIAMMLVPADTLV
                     MGNIAPEADDDEFPLLTISIPAFYIGQKEISQAEWMMVFPNNPSHSKDPALPVENVSF
                     YDIIEFCNQKSVLDGFEPCYDYYDNEVVCNFSSNGYRLPTEAEWEFAAKSGKRYDFFV
                     YSGSADPDEVGWYNGNSEAQSHPGGQKKPNQLGIYDLSGNLFEWVWNWYAPYSARNWN
                     LITGPDKGTDKVIRGGSWYHSVSEMRVTNRNYAKPYTKNAYLGFRVVRTKS"
     assembly_gap    220273..220372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            220557..221633
                     /gene="prfB"
                     /locus_tag="PLB65_00860"
     CDS             220557..221633
                     /gene="prfB"
                     /locus_tag="PLB65_00860"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOQ77104.1"
                     /translation="MNKYLKFGGYFDPEPKKLLISELEQKMNAPDFWLDQTNAKKVSK
                     QLSQLRDELDHIRKMENIKGELETYLSLLEEDFNIDLLNEAVSELPRIQNFIEKAEIE
                     CYLNDKYDHNDALLTIHSGAGGTESQDWVEMLLRMYSHWAEKNNYSFNIIDYLPGEEA
                     GIKSVSVEIKGDFAYGMLKSEIGIHRLVRISPFNAQGKRQTSFASVFVYPEFDEDLDV
                     EIDPKDLKIDTFRSSGAGGQYVNTTDSAVRITHLPTNIVVTCQNERSQIQNREKAMSI
                     LKSRLYQYYEEQREKEKQNVESSKTEIGWGNQIRSYVFQPYQMIKDHRTNYETGNTDK
                     VMDGDLNDFIYAWLKYNAKQRING"
     gene            221626..222366
                     /gene="uppS"
                     /locus_tag="PLB65_00865"
     CDS             221626..222366
                     /gene="uppS"
                     /locus_tag="PLB65_00865"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOQ77105.1"
                     /translation="MAKQDYKQLLNELDRDKLPKHIGFIMDGNGRWAKKRNKPHLYGH
                     RAGVKSLRQVVELGVELKLQYLTFYAFSTENWSRPESEVKGLLWLLKDQLKKEIPELM
                     EQNIYAQFIGTSIGLEKNYWNEVSSLVAQTHKNTGMIVNFAFNYGGRLEIIEGFKKFM
                     SMNPDKWDKLTPEDFGNYLWTAGQPDPDLIIRTSGEKRLSNFLLWQSAYAEIYITETL
                     WPDFDKTEMIKALLDYASRDRRFGGRKE"
     gene            222363..223163
                     /locus_tag="PLB65_00870"
     CDS             222363..223163
                     /locus_tag="PLB65_00870"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOQ77106.1"
                     /translation="MNELFKRVLVAALLIPVVLIILYFQGLSLVITLAIIVFLGGLEY
                     IRMMHKAKIQISYVWIVLNCLFYFAIVYIKGRDFSLLWLALLFALIESITTWNAERSI
                     PRLFATFFCFVYTALFPALIARISLSYSEYNFLLALILMIWFVDSIAYFVGMKIGKHR
                     NITAISPQKSLEGFLAGILAPWLILIILYICKVRIMSFSYLALIAIAAGIIGQIGDLI
                     ESMLKRYCKVKNSSNLLPGHGGILDRCDSILFAGSFLYCALEILTKVR"
     gene            223169..224902
                     /locus_tag="PLB65_00875"
     CDS             223169..224902
                     /locus_tag="PLB65_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ77107.1"
                     /translation="MKKALMLIVLMAATAAIFAQAVDLFFSEYVEGTSNNKALEIFNG
                     TGDPVDLSNYTVKLASNGGTWSTTNILHMTGVLDDGEVYVIANSGAEASILAIANVTS
                     TVTYYNGNDVVGLFNGDTLIDIIGVYQQDPGPANWPVAGTPSGTAEHTLIRKPDVIQG
                     NTNFIAGAGTNQDDSEWIVHPQNYFADLGQHTFNPSAPEQAATPVFNPPAGVYANPIN
                     VTITSETTGAEIHYTTDGSEPSESSTLYTVPIAISSTTTLKAKAFAAGFNPSYTATAN
                     YLFPVQISNIAALRASTADGTTIYQLANEVILTMQQTWRHQKFIQDATGAILIDDYPG
                     VIITTYNIGDGITGITGTLNRYTTGMLQFWPTVDPGPATSSGNVVIPQLVTITELNHD
                     IELYQSRLVRLNNVHYASPSGDYAALTSYDLLDDTGTVVMRTQFSDADYIGTPMHQGN
                     FDVYVIVTQYNTAVQVTPRMLSDFNPVANDDEVLIPRNIELLGNFPNPFNPETTIRFR
                     MDKSAPAELTIYNEKGQVVKTINTITTQGINSVVWDGKDLSGKQVSSGVYFFRLKSGS
                     YSSTKKMVMMK"
     gene            225079..226245
                     /locus_tag="PLB65_00880"
     CDS             225079..226245
                     /locus_tag="PLB65_00880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOQ77108.1"
                     /translation="MQTPQKKLLLITDLYPNKFNPVNGVFVQKQAIELSKFYQVQVVA
                     ACDKNPYLIYQHHQDGFTAKIICYPYWQKFFLSSLITYRILALPAIKRCYKRFQPDLI
                     HIHDYRHIPELFWLKPWLNKLPIPRFLTLHNIRTHPAFLINNPRIKFYKWALQWTLTR
                     WNHIFTVNSRLAEWVKTYNQEVTVIGNGITEPISVEPVLIDKFKKKLYDDYFKIISIG
                     NLVTEKGFSYLIEAVSILKKEGFLIQLLIVGEGEKRNDIERKIHSCNVAKEVTLPGRI
                     DNAFLRNILPLFDLFVLPSYSETFGIVFLEAMYAGLPVIGIRNEGIYGLAEDGVEALF
                     AEPKNSKDLADKIKFLINNPDLKEEIAKAGQRLVKEKYMLKELIQRVIAIYEQR"
     gene            226232..227521
                     /locus_tag="PLB65_00885"
     CDS             226232..227521
                     /locus_tag="PLB65_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77109.1"
                     /translation="MSKDKLLMIVNEFPPTGASSVQRPLKFAKYAVKAGWEVHIIAPK
                     KPVRKVLDYSLLQEIPPETKVYRVAGLGIKNPEENKMVDANFEDTAPQSKIIKAFWRF
                     LKLINDFLFPYDKQIGWMPFAYWKTVKLINKYKIRNIYITAYPYSAFLVGIALKKKYK
                     DSIFWIADYRDSWQFGPLLDKMVLPFRFKKICRTDDRVLVTCDAAVFVTPESRNIYLH
                     KHPWLDKKSYYIPNGYDEDDFEGVIPKKFDCLTFVYMGKLTRVYGNPISLLEALKECN
                     LPKYQVIHIGSISAAFLQDIQRIDKGVYKFCGYKNHREAISYILGADINLIFLNDDPI
                     STFSFTGKLFELLRAGKPILAMGPKKCIIEDLLKETGAGKYAWIKDKDQIKMKINELL
                     TDTSSFSIQTDIIKQYSRAETCKTLLSIYERGQISNS"
     gene            complement(227503..228549)
                     /locus_tag="PLB65_00890"
     CDS             complement(227503..228549)
                     /locus_tag="PLB65_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOQ77110.1"
                     /translation="MKDEPVKKVLIIHTWGIGDLILLTPVLQAVKQMYPKLELEVLFF
                     PESAAIPVTNAPFISKIHFTGWKANLLIPTILALRKQKYGAVLFSSGVKPWKTWLFML
                     LLKAEKKIGEYRTIRYPGLSMYIKFNPAFSRTRSNYALFESFLNLPSWEDALSKQNDL
                     HLYPYFHLTEENQKWAEKFLLENKLSGEKVIGIHPGCLAKNKHRRWSKEYFITLINKL
                     QEHYSYPVLIIAGPDEIEVGKAIQARTNTLILSNAALANVAAVISHLHFFINTDSGLG
                     HIASCFGINSLTIFGPANEAQTAPFSANSHIIRYPVSCAPCLEKKKKPKCALDCLVKL
                     TPDMVFKRVQELLI"
     gene            complement(228560..229903)
                     /locus_tag="PLB65_00895"
     CDS             complement(228560..229903)
                     /locus_tag="PLB65_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HOQ77111.1"
                     /translation="MSQINLKKNIITSGSFRIVILILSFLISWISARYLGVTLKGEYS
                     YLITLCGFMWTVLDLGVYRSYPYIVRKRPDEVQTLFGWTIITMFFEIVVMTSIGVAFL
                     DFWNKLIGYQLSPVKIIFLVMFVSLNKAFMQLQAIYMGMNRVLENSISHFLISVVFFI
                     LLLIGYFALIDVDRIDILLLFNLIGLACSVLYLAFRNRWGNWYKELNIKTIKLFYSYG
                     FRVFLSSLFIMLLIRADIVLIKHFLDFSEVGIYSLAAHIVDFIQIASNLVGGLLFVKL
                     SDIEDDISKWLLLKKTLLIFFFFITLANLGFGLIGKPVMQIMFGADFVPVYYVYFWLM
                     PAAYGLSFGSLFNNYLNSKGFPIISIILPAIALVLNITLNMLLIPTMGINGAALATSI
                     SYFLWFLLIVYFEQRDSKGKMLHYLMPTWKDVTDLYRETVIEIKEMMNNISHKKA"
     gene            230112..231260
                     /locus_tag="PLB65_00900"
     CDS             230112..231260
                     /locus_tag="PLB65_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ77112.1"
                     /translation="MTIAKIVFWVSWLLLAYHLFGYGLILFIFNALFTKKKSLYPEPD
                     EYPSITVLCPAYNEEKLIEAKIQSFLNLDYPKDKIKMIVISDDSTDKTNEIVEKYTNY
                     NISLVIQKPRAGKQNAHNLVLPMLDTEYILSTDADCLFEPDSVKILVANMLSDRRIGL
                     VSGAVKKVDKGNRESGEGIYWKYESFLKLMESKFKTIIGATGVMYLIKRELFTPISSN
                     SPDDFERVLLIIKNGYYATYEPRASISLEVTEKATEEVQRKIRIITQEWFVLKRNKEL
                     LNPFRYPAVSFIFFSHKLLRWLFFVFVLTGFISNAFLISVGFYRVVFILQIVVYLLGT
                     LGLISQAKGKHIPLTGLAGYFVAMVYSSAVAFFHFLQDKKYDLWNPLR"
     gene            231271..232263
                     /locus_tag="PLB65_00905"
     CDS             231271..232263
                     /locus_tag="PLB65_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ77113.1"
                     /translation="MNILITGSNGFVGSNLMWKLEEEGHQVIGIDISDQCDYKKHSET
                     MIGDIRSLKELNKVSDTYLKKYNSPIDLIIHCAAAKNDFGISHAEYYSHNKYGTKTLI
                     SFANQQNINKMIYFSSVGVFGHPQGEANEDFPYKPDSAYGASKLAGELLCIKWQQENP
                     KRELVVLRPAAIFGPRNITNTYKLIDTLHRRPYLTIGEGNHIKTIVSIYTVIDMTCFA
                     IQQLKPGFEHYNCIDEPYLTLKELMELICSNPGFKMPRIKIPLKAAIGIGYLFDIPAK
                     LFSIDIPVNSDRMRKLGTATYFTAEKAKKAGFIQRVPLKDSIVEMCNWYLSLNE"
     gene            232281..233309
                     /locus_tag="PLB65_00910"
     CDS             232281..233309
                     /locus_tag="PLB65_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOQ77114.1"
                     /translation="METIKIGIIGCGRISKNHFDAVSQIQEAEFVAACDVIDSRLQTV
                     SDNYGIKKLYTNYKEMMEKENLDLVSICTPSGLHPQIGIDAANHRINVLTEKPMATNI
                     EAADALIRACDANNVKLFVVKQNRLNSTMQLLKRAIDKGRFGRIYLAESNVFWQRPQA
                     YYDAEKWRGTWEFDGGAFMNQASHYVDALYWLLGNVDNVMAYTATMARRIEAEDTGCA
                     ILHFRNGIIATLNVTMLTFPKNFEGSITIIGEKGTVKIGGVAVNKIEKWEFEDYDDDD
                     RIAQDANYQPPNVYGFGHNPYYRNVIDVLLGKDVPSTDGRDGRKSVEIIQAIYRSAKT
                     GKRISLPL"
     gene            234333..235055
                     /locus_tag="PLB65_00915"
     CDS             234333..235055
                     /locus_tag="PLB65_00915"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOQ77115.1"
                     /translation="METREGMPLLGDFFPEMTVVTTKGIMNLPKDMAGKWFVFFSHPA
                     DFTPVCTTEFVAFQKLYPKFKALNTELIGMSVDQVFSHIKWEEWIKEHLGVEIQFPII
                     ADTGAIANKLGLIHPGKGTNTVRAVFIVDDKGKIRIILYYPQELGRNMEEILRAVEGM
                     QFSDNNACSMPANWPKNELIGDKVIIPAASTVEAANERLAKIKKGEIEGYDWWFSYRD
                     AKAKQVKKQVNPSKATTKSRKK"
     gene            complement(235119..235787)
                     /locus_tag="PLB65_00920"
     CDS             complement(235119..235787)
                     /locus_tag="PLB65_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77116.1"
                     /translation="MTKEVQLKNRFIVISCLVIVSITFIYLYFAVTSPEGRNCLSSSF
                     IDLSEEPVQKVNKDAYIPNIVLGSREYTIIPLAEYRIAGMVVSKKRYHRGFMSDLSPY
                     DFALIWGKTSEYLPYLKFDQIVRFCLYKYKTGAPIDIQYLNEHFSNNHLIPANKNIKQ
                     AMGKVKKKDIVQLEGHLVNVLGRDKKKRFSSWNTSTTRTDEGNGACEIIYVTCLRINH
                     KEYF"
     gene            236048..239053
                     /locus_tag="PLB65_00925"
     CDS             236048..239053
                     /locus_tag="PLB65_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77117.1"
                     /translation="MKKSFLIMVFMIVISILNAFERVPIYSSQYLEYDGNGITLPNGD
                     FLFFTNASTNNGLNVMMNRVCVNGTNVNSEPYNITNAPGDESIIQQVLSSDNCLIFIY
                     NYINPLVSYDDSEIYIQKVTLNGELLWGERGIRLGIPSYNRFKLVPNNNGGAYVTYVL
                     VRNYPKLYGWNFDAQGNNQWHSEFVFELQPPNLFYLSNVVSDGDGNIIVNLWVQPDSN
                     SYLYSYLVKISPQGNTIGNNPMVTQSQFSGCYDIFSAQNGNYFLWKSNLSSITFQKMD
                     ASGNLLFPEPISYQYDENVSEISTFGSSADGGLYYYLNREYPLYPMLCKLNADLQPSW
                     SAPIELPFYWNYYNGTYRIMDDNSIYIAYVDYINSYYPIYDSGIMLTHIDPINGTYSF
                     PATYISNMVFPKYGVHIFNSSANALVSWSDLTDGNFEVRCQIVDPSGSILLQTEGETL
                     QYRHFGSVEKYQAVEILEGSCYVFEINNIDLSSKLYYQICNDNLSPLLEEFGRELNPD
                     SDEIELLKAIVRTLDNQIGILYLSGNEYFNLYYQVIDNMGIALLPGKGVLIKENVANT
                     NCNFCMTCADGDVIIAWEEVYSLLYTQIIRGQCLHNNVPQWVSGGKILKQSTPKGVAL
                     IKILGNYLVFADYALDYSLGVIKLDANGNPEPSWALTGSYITSGTNLDLIQNCGLIND
                     ELVLFYYNQVSPDINNVHNLCVQKFNNLGYGLWGDYGIILDTSGSWGIASIEGASYGQ
                     KVFFSMLKYNVEHNRYEIHYNVINDDGTMLWENTESIPIQYIYPSSTLHHLSYNNDVN
                     SLFWLSRTDNYLKEIKHHYILPDGLLYYNEPVLIEQIAKAITNLMSLNKETYGLATYI
                     INDYYFEDKKFRGENEGLPTFGNNPKGISYSLNSLYACKISSNPTLAEDPIQNPSNLF
                     CHNYPNPFNPETTICFDLPAPDRIHLNIYNLKGQLIRNLINDYKPSGKQSVIWNGTDN
                     NNQSVASGVYVYKITTGKYIQTGKMMLMK"
BASE COUNT        77910 a        41393 c        43236 g        76547 t
ORIGIN      
        1 cggtgtttgt atagtgaatt aacaaaaact taaaatgctg gaatagaatc tttgtacatt
       61 aaaaacctta tacactacat aaaaaacaat gaaatgcaca aatactattg aagagtataa
      121 gttcttgaac ttaagttaga ggtataacga gcatagatga atatagttac gatatattat
      181 agtttattgg atcgtaatta ttggatcaat gatagagtaa tattacttga ctgtaagata
      241 gattgttata gaaaggctaa aatagattat tgataacagg ataaagagat aagtgaaaga
      301 agcaagttcg caaatagagg attttattaa gacgttccga acggacaagc gttttaattc
      361 aactattgta gcagaaaata ttaaagaaga gaagcaagca aaaacagccg attttcctaa
      421 gggtttactg ccttatattc ccagtttagt caaaagtatg gggattaatg agctttatca
      481 gcatcaggca gaatctatta aagagctttt ggaggggaaa aatgtggtgg ttagttgtgg
      541 cgttgccagt ggaaaaagtt tagtatatca atcggttatt ctaaatcaaa ttatccagaa
      601 accacaaagc agggctttat tactttttcc aacaaaagca ctagcacagg atcaagccca
      661 gaaaatgcaa agtttatgca acgaactatg caagcaaaat cctagaatcc cttcaattat
      721 aaatggaatt tatgatggcg atactccttc agaaataaga ggaaaattac ggaaacaagc
      781 caacatattg tttaccaatc ctgatatgtt gcatattgga attttaccaa atcacacttt
      841 atggagtgca tatttatctc gtttagttta tgtagtaata gatgaagttc atatataccg
      901 aggtgtattt ggctcgcata tggcaaatgt gatcagacgg ctaaagagga taacgaacat
      961 ttatggtcaa aagccacaat ttatatttac ttctgcaact ttagcgaatg cgcaagagct
     1021 tgccgaaacc cttattgaag agccggtgga gcttattgat aatgatggtt caccccaagg
     1081 tgaaagacat ttttatatat gtaatcctcc aatggtagaa ccttctttgg gaataagaag
     1141 aagttccaca atggaaatta gcagtatagc caaaagcttt ttacgcacaa acttgcaagc
     1201 aatattattc acaggaagca gacgcagtgt agagcttatt tatcggtatt tggttgatga
     1261 taaaaaagtg gctgataaaa tacggagtta cagaagtgga tatttagctt tggaaaggcg
     1321 taaggtagaa aaagaattaa gggaagggaa gattggttta gttatttcaa ccaatgctct
     1381 cgagctgggt attgatattg gaggtttaga tgctgttttt ttaaatggtt atccaggaac
     1441 catatgtgca acaagacaag aagctggaag ggctggacgc aaaggtaatc cttccttttg
     1501 tattttagag gccggtagta atcctcttga tcaatacatt tgtcagcatc cggaatatat
     1561 ttatgaaaac aaccctgagc aggcattaat agatcccaat aacagtgaga tattaaggat
     1621 gcagctgcta tgtgcgatta gtgaaatggc gttcaaagaa ggtgaaaatt ttggtgcttt
     1681 aagttttgct gaaattcagg gttatcttta ttctctttta gatgaaggtt taataaagca
     1741 tattagtgat cgctatattg gaattagtaa tagttatcct gctgcagatg tttctctacg
     1801 caatgcagga aaccagtttc aaatcttggc agataatgaa ttagtcggtt gggttgattc
     1861 tggaagtgta aaatggatga ctcatccaga agcaatttat cttcaccagg gtgaaacctg
     1921 gattgttaaa gagcttgatt ttggacaaaa aaaggtcatt ttagagccga ttcaggttaa
     1981 ttattacact caagcaattc aatatacaga aataggtttg gatgatttgc tgaagctgga
     2041 aaatgttacg ggtggtcgta aacattttgg aaaagtaact gtttcccgca caataacagg
     2101 ttttaaaaaa ctgcgttttt atacattgga agttttagag caggaagaac ttgatttacc
     2161 tcccgaagtt atgcaaaccc aagcatattg gatttctctt tcaggggaaa cagtagaaaa
     2221 aattcgtaac cagggtcttt gggctaatga taaaaatgat tatggacctc aatgggaaaa
     2281 gctaacagaa aaaatacgcc aaagggaaaa ttaccgctgc agaaattgcg gtgttacagg
     2341 agatttggaa gtccatcata taattccttt tcgtcgtttt gacgacccag atgaagctaa
     2401 tgatcctgat aaccttgtag ctctttgtcc ccgttgtcat aaattagctg aaacaagagt
     2461 tcatatccaa agcggtttag ctgctttagc atatttgctt ggtaatttag cacctttttt
     2521 tgtgatgtgt gctcctcaag atttgggaat tcatagtgaa gacaagtccc ctttggcatt
     2581 aggaaatcct gtaatagcta tttatgataa tattcccgga ggtatagggc ttagcggaaa
     2641 actatatgaa ttgcataatc aattactata tgcagccata gatagagtgc aaggttgtag
     2701 ttgtgagaat ggttgtccct cttgtgtagg accagtagca gaaaatggtt tgggagcaaa
     2761 agaagaagca atagcaattt taaaggaact gatttcataa atgagggagc agcaaaattc
     2821 ataatcggga tataattcaa tttgctatat gctctatagg caattataat atagataaaa
     2881 tcgccaatgt aacaaaaaaa tgatagataa tgaaggaact atgaaaaaag caatgtcaat
     2941 ccttgttctt ttgctaatag taacaatact ctctgcgtta ccaaaagaac ttacccgatc
     3001 cggaaatcct gagctctata aagaattttc taaaaaagca agagtaaact taaaacagct
     3061 gcaatataag caaaaggaag cataccaaaa aatgttaaaa gaacatccag atgttttaat
     3121 gtgctatctt attgcttatg aatgtgatgc caatttggca gctgccgatc cagaggacat
     3181 cgaaagtaac tatcaaacaa tctgtgctct agtaaataaa gaagggttga aatacaaacc
     3241 tgagtttttt ctttcttaca tagcgaaaca gacagtttct gatgaagcca ttactgcata
     3301 cagaaaagca atgctaaagg atggtttaca gaatattatt aatactaccc ataatgatat
     3361 agacagattc agggctgtaa ctttatggtg tgtttcccgc ttgcaatttc agcaaacttc
     3421 aggaagggat caaacgccct tggatataat tcacaagtcg attattgggc gctgtgaaga
     3481 aatgcaaatt ttgttagttg ctgcggccag aacagttggg ttaccgtctc gtccagcttc
     3541 agttccttgg tgggctcata ttgataataa tcatgcctgg acggaaatct ttttggatgg
     3601 tgaatggcat tatacaggag atatggatat ggcatatttt cctgatcaaa cctggtttag
     3661 tggtttagtt gataaaactg ttatgatttt ggcagaaggt tctttagcta cttgggatga
     3721 tgaggtctta gctaaaggaa aatatgaaac cctgataaat tctacgcgta attataccaa
     3781 agagcgaagt agaaccataa aactaaaagt aataaacgaa aatggtaaac ctgttactaa
     3841 tgcttatgtg attccaatgg tttttaattg gggatcttta agagctttaa ctgttttaac
     3901 tacaaataaa aatggctatt tacaattcac tgctggtcgt ggtgcttttt atttatctgt
     3961 ttatagcaaa gatggtaagg cattaatttt agtcccttcc aacgagaaaa caaatgttga
     4021 attgacagct aagctcgata ataacgactt cccagggggg tgggcaactt tggaatatcc
     4081 gggtaatatc cataattggc aaactcaacc tgaagaatat accttagcgg taaatctgga
     4141 aaaacaactc tggcaggaaa agcaaaaggc atttgaaact aatgctataa aagcaaaagt
     4201 aaaggatgat tcgcttatga taaaggttgc tattgcctgt cgaggtaatt ttactgcttt
     4261 ttgggatttt gtaaaacgca atcctactcc ggaagcaagc tttttagaat ttttattatt
     4321 aggcgatccg aaatttttgt ggcaggcgaa agcggaacaa tttgaagctc tatacgagaa
     4381 ctggatccgg ttacaagatc ccgatataga tgaagagaat accctaagct tattttcttc
     4441 cagtgttttt tatgaagact taccagaacc gattgaatat gtaaaaggca aggcacaact
     4501 ttatcccaaa gcctttagat ttaattaccc ccacaatcgg gaaggggcta tcaaagttct
     4561 atcaaaactg aaacaaaaat ataaaatcaa accagaaaaa gctctaagtg gattgccccc
     4621 tttacatata gccatagagc ataaatatct tactcctgtc caatatagaa taatggctgt
     4681 ttatatatta cgagctaatg gttttcctgc tgagttttct cgaattccaa atttggtagc
     4741 ggtttttatt gatggtgatt ggcaatatgt aaacattgtt aaactttctt gggaagatcg
     4801 ttccaaagat gataaagcca gtactttcca ctgtacttta cagattaatg atacagataa
     4861 taatcctatt aaggtggaag aggagcagct tactctttgc cgttatgaga atggaatgtt
     4921 ttacccgcta aatgttaatt ttgattatct tggctctggg aaatatacag gaattttccc
     4981 cgtttatgaa acctggctac agtttggata tagggtttca gacagcaaaa caaagttcta
     5041 ttatcaacag ataaaaccta atattgatga tgttattcac ctggaaatta tagcagaaga
     5101 atatccccga aaatgggaag cagctgatag tgaactaatt gccctgcttg atgatatcca
     5161 aaactcggaa aataagatta tcttgattgg aaactacgac caagaaaata gccggcgttt
     5221 ggctgataag ctaatttcct tgaaaaaaga ttttgtttgg ttaggttata gtacttcaga
     5281 aacagctccc caaaattata ttctaagtaa caaatggaat tcgatggtac gcgaaaacac
     5341 gcataatgct atgcgtagca taactttaat aaaaattggt gagacctggc aaatgtatga
     5401 tggattgtgg gataaacttc ccgattaaaa atgattatta tagattagtg tcgctaacag
     5461 actactaaaa tttcacggat aaaaaagaca ttaataagaa aggacaaatt aaatgcccga
     5521 attgcctgaa gttgagacca tcgttaaggg cttgaaaaaa gttctgaaag ggaaagtaat
     5581 aaactctctt gaatgttact atccagggac tataatttat gatactgata tagaggcaac
     5641 accttttcca gcaaaggtgt tggagctttg tcgcagaggt aaatatatca ttatcactct
     5701 ggaaacagat aatgccatta tcattcatct caggatgact ggtaaactgg tttttgatgt
     5761 tcttccaagt gaacctctac aatacgaacg cgccaggata attttgcaca atagtgaagt
     5821 tttgcatttc atcgatatac ggacattcgg caaaataatc atttgcaagc agcagaatga
     5881 agaacagtat ttacctattt tgggaattga accttttagt aaaaacttca acgctaacca
     5941 tttaaaagca atattgaagg gtaaggaagc ttcaataaaa accgttttgc ttaatcagag
     6001 tattattgct ggattaggaa atatttatac ctgtgaaatt ctttatcgag caggaataaa
     6061 tccccttatg ccagcaggga aacttagcca taaagaaatt gtggaaatag ttcaacaaac
     6121 attagcagtt ctttctgaag caatatcctt gggaggaaca agtatctcag attatcgtaa
     6181 tatagatgat aaaacaggca gctttcaaga tttcttgaag gtatatcaaa agcaattctg
     6241 tcccttaggg cacaagatag agcgcataaa acaatcagga cgctccactt tcttctgccc
     6301 aatatgccaa aaaaataaaa gttgactagt taaggtaaac gatatattat gtaattatgt
     6361 agaaaagtta gaactgctta taggaaatct gcaaatataa ttcataaagg agatgtaagc
     6421 atagtgaaag cactaaaact gaaagaaggt atatattggg ttggagctat agattgggat
     6481 ctgcgtaatt ttcatggtta tgtaacacaa cggggaagca cttataatgc ttatctgatt
     6541 atagatgata aaataacact tatagacaat gttaaaagct atctttatga agaaatgata
     6601 accaggatca aggatattat tgatccttct aaaattgata tcattatcca aaatcatatt
     6661 gaaatggatc atagcggagg tcttcccgag ctaattaaat tgatccccaa agctacttta
     6721 tatacaaatg caaacggggt taaagggcta aaaaggcatt tcaagcagga ttggaatttt
     6781 caagaagtta aaaatgggga gagcataagc atcggtaaac gcactttgag ctttttaact
     6841 actccaatgg tacattggcc tgataatcaa tttacctact gcccggaaga aaaaatactg
     6901 ttttctaatg atggttttgg tcagcatatt gcctcaacag aacattttgc taaagacctt
     6961 cccaagggaa tagttttaga agaggcaaag aaatattatg ccaatattgt tttgccatat
     7021 tccaaacaag ttcagaatgt cttaaaagtc gcaaaaggtt tagatatcca tatgatatgt
     7081 cctagtcatg gagttatctg gacagaggag aacattccag aaattatatc tgcctatata
     7141 aattgggcaa ataatgtttc agaccgtcac cgtgctttag taatctatga tacaatgtgg
     7201 aagtctactc agaaattggc atatacaata tctgaagcat ttgaagaaga gggtgtaagt
     7261 acccagctga tgaatttgca aaccaatcat atatccgata ttatgacaga agtaatggat
     7321 gccaaattca tcgctgtagg ttcacctact ttaaatagtt ccatcctgcc aacggtggct
     7381 gcttttatgt attatttgaa gggcttatcg cccaaaaata aaattggact tgcttttgga
     7441 agctatggat ggggtggaca aagcatacct attttacaag aacttttgca agatccggaa
     7501 caatgtggtt ttgaaatgat ggagccgatt aaaacacaat atattccaga ccaggaagaa
     7561 ttaaacaaca tcaaaaataa cttgcggcag caaataaaga ctaaactgga agaataatgt
     7621 tttgccagca gttattagcg aacggcaaaa taagaattca taccattaag gaaagaacgc
     7681 taacttatta gcaatggaga ttaatatgaa ctcttgtaat gaacaagata tttattcatc
     7741 cgtatttcac agtgccttag tagctattgg gatcaccgat gttgagggta attatgtaat
     7801 cgttaatcct acctggtgta aactaatggg ttggtcagaa gaggaggcta aaaaactaaa
     7861 tatcagagat ctaactcctc tagaagatca aagaaccagt gccgaaagct ttgaaaatct
     7921 catcaccaat aaagtaaata gtatgcgtaa acaaaggcgt tataaaagaa aagatggcag
     7981 cattttctgg gctgatctgt attgctctac attatataat gatgagggaa aggttatggg
     8041 ggtactgggt atatttgtcg atatcgataa acagataatt ggtgaacagg ttcaaaaaga
     8101 attatatcgg aatttggaaa ccttgaatat ggaattaagt tccaccaacg aagatttaaa
     8161 acgccttgcc agatatgatt ctttaacggg tctgtttaac cgaagagtta tggaagaatt
     8221 actaactaaa gaaagtagcc gtgcttttcg tactcatcga ggttttggag tcgctattgc
     8281 agatatagat gattttaaaa aagttaacga tacttatggc cacgattgtg gggatattat
     8341 tcttattgaa attgctaata tctttcaaaa aaacattagg ttaacggact cggtaggtcg
     8401 ttggggtggc gaagaatttc tatttatcct cactgaaaca acagctgaag gtgcttatat
     8461 agcgatggat cgtattcgca aagctgttgc tgctgaaatt tttgattatc ggcaaaatcc
     8521 tatagaaatt actttaacca taggcttaag ttttcaccat ggtgatccaa gtagcaagga
     8581 aattgttaat caagccgatc aagctttata tagaggtaaa aagaacggta aaaatcaatt
     8641 agtaaattac tataacgatg tgtcagcatc ttatatcact gaggacaatc ctacaacata
     8701 agtaaccgat gattatactg tatatcttaa atttcaagca gattttaata tgctaaaatt
     8761 ctgatggatt gcagttgtaa gagttcacag tagtaagtta attaatacca tatttaattg
     8821 taaacccctc aaaacgctgt ttgttttaac cataaattgt gctaactact tatataatgg
     8881 acgcctatca taaatctata gttatgcact cttcattata gatttatcat ttttcaaaat
     8941 tgagagaaaa ataaagaact caattttagc ataccttaaa atagccaaaa agtaactata
     9001 tgcaagtgtt caagaaagct ttacaattga ttagctcact gtatcgttaa gtttcagagt
     9061 gagaaaattt aattacggtt ctttcaggtc tatttggatt gaaggcgagc aaaagaaact
     9121 gttgacataa aagtaagcaa agctattttg tcttcaaatt gaaaaaaaat ttggggataa
     9181 agaatgaaga caaatgatca gagttggaaa ccaactctta ccttggctaa actttttgaa
     9241 gaacagaatc agttttacga agcattggca gcatatgaag taattagcca aaacgaatca
     9301 tctccagaat ttagagaaaa gatcgaagag ctatatctgc gtattttaaa tgatcctaat
     9361 agcgattacg atcccagagt ggaagaactt tttgctcctg aagaacttgc ttatcttaga
     9421 attatgaatc atcagggctt taataattta gctcaggcaa gagcaaaact tgaagaaggt
     9481 atgcagggtt cagagataat ctttagccaa gttatgcctg aagatgtttt atccgatgaa
     9541 tctgaagtcg aggtattaac ttcaattctt caagaaattg aacagcaggc tcagttgaat
     9601 attgtttcgg aaaagagtga ttttgatgag tacaatgttc aagatcttct catagcgatt
     9661 ttagccaaat tcgataaaaa tcagaaactt agtgaggtca aattgagtga cctcgttact
     9721 ttatttctgg agatgcagag tctaaaaaca taatttaaat gtttacaaaa cttccaaatt
     9781 cagtgaattc tctttgtgct aactgctaca ataaatgcaa acaaaatgat tttgtttcaa
     9841 ttattatttg ccctttttac gatccgaagc cagtgcaaat ggaattgata tttaaaaaaa
     9901 ctcgtaaaca acccaagaag aaagcaacaa aaaaatgaga ataataaccg gtatttacaa
     9961 aggacgcaat ttatttcttg tacctggaat aactactagg cctacaactt cttttaatcg
    10021 cgaagttatt ttcagtgtgt ttcaaagcta tcaaggatgt agagttttag acctttttgc
    10081 cgggacaggt tcttttggtt ttgaggctct ttctcgcgga gcaaaatggg tcgattttgt
    10141 tgagtttgct cctgctgcag taaatacaat tctcaaaaat atagctcttt taggatgctc
    10201 cgataattgt catctatggc gaaagaaagt ggatgtgtat ttgaaatcct gtaaagagaa
    10261 atacgacata atttttcttg atccaccata taacaagaac cttatcaacc catcattaaa
    10321 actcatctat gaaaatgaac tgcttaatga aagtggtatc ttaattgtag aacattctcc
    10381 taaggaaaat atcgaagttc cttattctga ttttataaat actgaaaaat actttaaaaa
    10441 caccaatttc agctggttag atagtaagct ctatccaaaa taacaatctt ctaagaagat
    10501 ttttcctgtt aacctaaaca tatcttgact ggggtgcata ggaaaaagta cttccttctt
    10561 taataagttc agttactaag gaagaagtaa tattctgttt ctacaattta ccaggtattt
    10621 actacttcag cgtaaattgc atattgtgtg caagttccgc tcttcgccca taatagaaag
    10681 cgttaatcaa gtgttaatct aaccttaatc aagcgttaat tattaacgct tgattaaggt
    10741 atgttaaacg atagatgaat ggatttaagc aaggtatata ggagataaca atattgggat
    10801 tttatgataa aagtgattgc taaggttggt ataatacggt ttctataggg aaataatcac
    10861 agcatttttc tattttaact ttcaatacat ccaattggaa ttgatactta tatctatctt
    10921 ataggaatga aaacttaaga ggggaaactt tttactatta cctctcataa atatatcttg
    10981 acttattacc aaggttataa acaaaagtat taaaattaag aataatggtt accacatcaa
    11041 agcctattat atttatcaga atagaataat atatttcagg aaaaaacaat gcgaagcttt
    11101 agtacgatct ttacagtatt gttacttttg ctattaagct ctgtagcaat agcacagaat
    11161 gtttccaaca ataattctgt cttgttgagt gaccaagatt tccctattgc caatgtagat
    11221 aatatgttca ttcctgtcag caatccttct ttgataggga ctggaacatc aagtggagtt
    11281 ggacttgctt atctaaacga tgaaaaaaag tggcaggatc actactggtt atttgtgaat
    11341 acggatttcc ttagttacat ctatgaaaag gatcatagtg ataattttca caccttggct
    11401 ttaggtacgg aactttttcc tgccttcatt ttacccaatt tatatgtagg aacaaattac
    11461 cgctggctgg aggatgattt tgaaaacgga atttttagaa gtggaataac atacagaccg
    11521 cataattcta cttctgttgc ttttacttgg gataatccca aacataattc tccttattat
    11581 agattgggtc ttgcttttcg tccttttact ttttcagagg caattgccga ttatcgttta
    11641 gaactttcct tagatgcaaa ttatgttcga tacgaaaatg ataacgatta tgagattaaa
    11701 aagccggtta tcggattgca aactcaaata ttagacggtg tgaaaattgg agcaacatat
    11761 aatttggata aagaaactgc gtttgtcaat ttcagtttat gtgcagccaa tttggaagca
    11821 ggcagtcttt tacattccaa agataatgat aattatggaa ttgcctgggc tcagcttacc
    11881 tcattaaatt acaagccatt tttaggttat aaagattctt actggtacaa aatggattta
    11941 acaggaaatg ttgtaaccta tgcagctcct aaatttaaaa ttgggccttt tagtttatac
    12001 gatacgaaag ataaatctat cgaaacgata attaatgaaa taaacaaggc aaaagaagat
    12061 cctaatgtgc aaggaatttt gttaaaaaat ccatcgtttt ctacttcgct ggctcttcaa
    12121 gaagaattga tagccacttt tttggatttt aaaagcaccg gcaagcaagt gtgcttgtat
    12181 tatgataata ttggcaatgc tggatatatg tttgctgcag caattgctga taagatatac
    12241 ttaaatccta tgggttctgt cgatttacga ggtttaagca ttagcagtcc atacatcaaa
    12301 aatatgcttt ccagtttagg tatagaagtt cttaattttc gcagccataa atataaaaat
    12361 gcaggtaata tgttctccga agaaagaatg acagatgcag aaaaggaagt ttatgattcc
    12421 attctgcaat ccctctacgc tcaaatttta cagcggatag aagttggacg gggagataaa
    12481 ttaaccgctt ctcccgatga aattattaat gcaggaccct attttatagc aactgaagca
    12541 ttagataatg gtttggtaga tgctcttatt tatgaagacc agctaaacaa acaaatgaaa
    12601 aaagatttta aatttgttaa aaaacaaaaa gaactaacag actatcgtga ttatgcttgg
    12661 tcaaaaccaa aggaaaatca aattgcagtt atttatgcca gcgggaatat agtttctgga
    12721 aaaggaacac caggacaaaa aatagctcaa gaaactactg tagatctaat ccgtaaagcc
    12781 cgctataata atatgtataa aggaattatc ttaagagtag atagcggtgg aggaagtgca
    12841 caagccagcg atattatttt aagagaattg gaacttgctc aaacggaaaa caaaaaacct
    12901 gtagttgttt ctatgggtgg gacagcagca agtggtggtt attacatttc ttgtaatgca
    12961 gataagataa tagctgaacc atcaacatta actggttcta tcggagttgt gggtttagta
    13021 ttcaatggca ccgaaatgtt taataaaatt aaagtaaatt gggataccgt aaaaaaaggt
    13081 gaacattcgg atattggatc atttaaccgt ccctggactg atgaagaaaa agaaatgatg
    13141 actcgcagca ttgaagatgt ttatgagacc tttgtaaaaa gagtagatat tggacgctcc
    13201 tctttggatt ataatcaagt gaatgaatat gcccaaggtc gtgtttggac aggtgcacaa
    13261 gcttatgaaa taggtttgat agatggtctg ggtggaatgg aaactgctaa agaaaatatg
    13321 caacaattgc ttggtaaaaa aggtaaaatc accttggttg atgccaccac taaaaaagaa
    13381 ggcgtaaaaa ttagtttcaa tatcagcgag atgaatacct ttactccttt aaaggcatat
    13441 aatgccgtta ataacgatta tataaaactg tatgaacttt gggaagatta tgctggcgac
    13501 aaggctctaa tgatctctcc agaactaccc gaaatattgc aattttaact tgttttaaga
    13561 taaaggggtt agcggtcgat attgtccgtt aacccttttt ttctcatagt ttttatcctt
    13621 acaattctga agcatttctt taacgcttcc ttaatctcgt taaggaaatg ttaaaggatc
    13681 attagggaat tgcttaagtt acaatgaatc gtcctattgg attataacat actacattgt
    13741 tggaaatgaa gttattataa aaaaggtagc acttaagacc attgcttgta atagcataca
    13801 attttgctaa tatcttattt ggaatacact tacctgaaat ccttattaaa aaaaggctac
    13861 acactattag ctttgggcaa cgatcagaat tcccgttccc tttttgttat ctgttttcat
    13921 atctatacgc atcaatattt cacaaacagg ataaatgagc aggtaataga taggtaagat
    13981 aatatacagc tttttggaag tgatctgcat tggcaatttt aacatcaatt tccaggataa
    14041 ggagccaaag gttccatagg tgaaaatact tttaattata ctgaatcctg cttgctgaag
    14101 tttttcttcc aaatcctctt tatggtaacc ggcacgaata tgttcggaag taaatttggc
    14161 tgcttcatca aggtctgaag gagtggaaat aattaaatat ccacctggct taagcgcttc
    14221 tttcaaattt ttgatagctg attcatcatc ttctatatgc tccaaaatat caattgctaa
    14281 agccaaatca tatttattgg caggtttgaa aatctgtaaa tcggcacttt tataaaaaaa
    14341 tcttccagga tatttatcag cagcatagtt agcaaaagat ctcagataat tagtttttag
    14401 gtctgtagca aaagcagtag attctggcca atgggaaaga acaaaattgg aatattgaca
    14461 aaaacccgct ccggcatcat atagttttat tttatcagta ttatgaaaaa gatttcgtat
    14521 ctctctaaat acatatctct ggcgcagaaa aatgatatta aactgtaaat aaagcagttt
    14581 tcgcaaacag ggaaacacag aaataacatt ttccagaagg tttttcaaag gctcgtattc
    14641 cataactgaa tctccttaaa caaaaaatac attgactatt tttagcaatg ccaaaataag
    14701 tcaactgaga aatttagatt atgatagtaa aagaaataaa tgatattaaa cggatcaatc
    14761 tgcatttgca tactaatgtt tcagatggaa gtttgagccc caaaacactg gttaataaag
    14821 ctcgggaaat tggtcttgat ctgatttcca taaccgatca tgatactgca gatgcttatg
    14881 ctcaaatccc ggaaaatgtt tcgcccttga gaattctacc tggtgtggaa ataagttctc
    14941 ggtatgaaga tgatgatgtt catattttag cttatggctg tgaccttacc aataaagcgt
    15001 tgcaggagat gatggaaatg tatttggtcg gccgaaaaga aagagctaaa aaaatgatta
    15061 gtattttagc tgatatgggg ataaaaattt ccctggaaga agtaatttct gttgctggaa
    15121 gcagagaatt gattgtccgt cctcatattg ctcaggtttt agttaagcac ggtttttgcc
    15181 aaacgaaaaa tgaggctttt gacaaatata ttggtaataa taagcctgct tatgtgccaa
    15241 agccagaagt gtctgttgct gaagcagttt ccattattca taaagctgaa ggtgtagcgg
    15301 ttgttgccca tcccgtaaaa ttgactaaat tgtcttatat tgacgatttt attccaatgg
    15361 gtatagatgg attggaagta tggcatccag atcattatca gtgggaaata gataacctta
    15421 ttaatattgc tatgaaaaat ggtctttata tgacaggggg aagtgatttt cacagcgaac
    15481 aggataaaca taacttattc gatatcattc ccctgcccga aattgttata caaagtatca
    15541 aagaactttg gaaggaatat caatgccgcg cactatcaag gtaaaattaa ttcgtaatga
    15601 atttcctgct acttatcgtt attcattatg gttgaggata ttcaaattat tatttgcatt
    15661 tgcagtatgt ttatatgcct tctattttgt agtgaaattt gtttctgctg aaacacctat
    15721 gttttttaaa gtgcttcccc tgataattct gttcattgca ctggatggta tgtttaaaca
    15781 acttatggca ttaaacagag ttactttcta tgaagataga gtatgtttcg ggttcattgc
    15841 taaacccagg ctgattattc cttacgaaaa tatcctctat atagatttag taaaacagat
    15901 aacatacagt attatactga aatacaaaga aggcaatagg gaaaagatat ttaaaacatc
    15961 tgcgtcgttt ccgaaaatct tggaaattat tctaaatttg gcagatttag ctcccaacgc
    16021 acaatcgagc gaattttttg gaaaaattat taaacattta cgaactcttg ctggagaaaa
    16081 tgatgcagag caggtttgac aaaataatcg atcgtaggaa tagtggatgt tataaatatg
    16141 atgcgctttc aatgatatac ggaaaaagtg atcttatccc tctttgggtt gcggatatgg
    16201 attttgctgt ttccgatgct attctggatg ctttatcaga acgcctagaa catcctgtat
    16261 ttggatataa ctttcgtttg aacgatttta gtgaagcaat tgtatcttgg gaacaaagaa
    16321 ggtttaattg gaatattcat aaggattgga ttatttctgt tccaggtttg gttccagctt
    16381 tatcattatt tgttcttgcg tttacagaac caggggaggg agttcttatt caaactccag
    16441 tttatcgtcc tttctatgat gcagttatag atcataaccg taaactaata acttctgcgt
    16501 taattaataa taatggagtt tacagcatag attgggatga ttttgaaaac aaactaaaac
    16561 aggcgaaatt gtttatttta tgtagtcctc ataatcctgt cggcagagtt tggactgaag
    16621 aagaactatg taaaattggc agtttatgct ctaaatataa agtaattgtc ttttcggatg
    16681 aaattcacgc agatattgta tatccaggtt ataaacatat accttttgca tctcttgctg
    16741 atttttctaa cttttgttta acaggggttt ctccttccaa aagttttaat atagctggtt
    16801 tagcaaccgc agtagtaata atatcaaatt cagttattag agataaattt atggttatga
    16861 atacaaagct tcatctatat ttgggtaata gctttggaat taaagctcta attgctgctt
    16921 acaacgattc agaaaattgg ttggaagaat tgctctgtta cctaaatgga aaccgggagt
    16981 acataagtaa ctttgttcaa aatgaattgc cgggagtttt actcagccca attgaaggga
    17041 cttttctagc atggctggat tttagaaact ggggtctgcc agagcccaag ttacaagaaa
    17101 tgataattaa aaaagctggt ttggcacttg aacctggaag tgaatttgga gataaggatg
    17161 aaggatttat gcgtttgaat tttggttgtc cacgcagtat tttagagctg gctttaagca
    17221 agataaaact tcttgctgag gacctttaat gatggaacaa gaaattattg atctctataa
    17281 aaatcccccc caaaaatata cggaagagca taaagctctg tttatggctt tttccgatgc
    17341 cttaaatagg ggtattgttc gttcctgtga aaagaaaaac gacacctgga tagttaatga
    17401 atgggtaaaa atgggaatcc ttactggttt cagaatggga gaattaaccg ctttcccttg
    17461 gagcgaaaaa aaacccttct tcgataaaga tacaataccc gaaaagctat ttacccttaa
    17521 agatagaatt agaattgtcc ctggaggaag ttcagcacga aatggttgtt atatagctac
    17581 cggagttgca attatgcctc ctgcctttat aaatattggt gcctatgtag atagtggaac
    17641 acttattgat tcccattctt tggttggttc ctgtgcacaa attgggaaaa atgtacatct
    17701 gtctgctgga gctataattg gaggcgtatt agaacctgta ggtttacgtc ctgtaattat
    17761 agaagacgat gtttttgtag gaggaaacac aggtatctat gaaggtataa ttgttcaaag
    17821 taaagcagtg atagcttctg gaactgtgat aactgcttcc actcctattt atgatagtgt
    17881 acaaggaaga tatttaaatt atgatagcgg taatagtttt actattcctt atggagctgt
    17941 tgttgtgcct ggttccagac ggttaaaaag taacccagaa tttcaagttg cctgcccaat
    18001 tattattaaa tatagggacg aaaaaactga taaagctgta gaattggaac aaaccttaag
    18061 agagttatta gaataaggta gccaccaatg cctccttatt ttacaaatta aagaacccta
    18121 agcttctatg ctttaggcag ctagataaaa gcaatattag gataaaataa tgaattacat
    18181 ctttactaac ggatttgtgc taaacagcca gacccaaaac tttgaagaaa aatccgttct
    18241 cattcataat gaccgaatcg ccttaatagg aaatctgcaa gaatgcaaaa tgcaggcaaa
    18301 atcatcttat gaaataatca acctcaaaaa cagattatta ctgcctgctt ttttagatgc
    18361 gcatactcat tttgtagaat atgccaaagg acgcatactg gtaaatttaa acggttgcca
    18421 tactatagat gaaatacgca attaccttat atcctataga gataatctta gctggaagct
    18481 aaaatggatt ttgggaggtg gttgggatcg aaactgctta aataatcctg acgaactatc
    18541 ccttaagctt ttggatacaa tctttccgga tattcctgtt gctttaatga gtaaggatta
    18601 tcatagtaaa ctatgcaatt ccttagcttt aaaaatagcg ggaatttgta aagatacagc
    18661 aaatcctaaa ggaggtttaa ttgagcataa tagtattggt gaacttacag gagttcttta
    18721 tgaatcagcc aatgaactaa tagatcccta tattgtctat ccggaatctg aagttattat
    18781 tcaagctatc tcagaaacgg tggattctat ataccctttg ggtttggttg gttttaacag
    18841 tatggaaagc attttcagca gagacctaat gttaaaaacc caagaaaaga gaaagaaatt
    18901 ccgtttttgc tggcattttt atccagagga ttatgaaaag gttttgcagg aaggtatcaa
    18961 aagctatgaa ggaaatgagt tttataaact tggtggctta aaactttttg gagatggtag
    19021 tttaggttct caaacagctg ctatgtttga aagttatccg caaggggaaa aaggtatttt
    19081 acgctacaca gatgatgaat tattttcctt agtgctttca gctgcagaga atggactttc
    19141 ttctacaatt cattcaattg gaaaccgctg tgtaaaacaa gtaattgatt gtttcctgcg
    19201 gctgaaaaaa actggtaaac ataataccct ttttaaccgt attgaacata ttcaggcaat
    19261 ccgtaatgag gatatccctc tgctaaaaag ttctggacta tttgcatcgt tacaacctgt
    19321 gcatatagcc aatgatattc ctctaatcaa taaatactgg agtgatatta aagaacaaac
    19381 atattcaatt aaaagcctaa tttctacagg aattgaatat gcctttggtt cagatgctcc
    19441 catagaaact atcaatccat ttttaggaat ttactctgct atagaaagac gccaaaataa
    19501 taatcctaaa aatccacaat ttagaccaga gcaatcaatc aatccgttcg aagcaatttg
    19561 gggatataca atgggagctg ctaaatctac aaaaagtgaa cataaacgcg gatcaataga
    19621 aacaggtaaa ttggcagatt taatcgttat tgaggacttt agacaatacc cttccacttt
    19681 ttggttaact gcaaaaagtg aattaacgat gataaacggt gatattgttt atgttaacga
    19741 agggtagaat ctcgttagat aacaaaaagg gtaaatcata gcttatcgtt cttctcgtaa
    19801 ataataattg tgttactact cattttgttg cgaacaaaat aataaggcag ctacccaaaa
    19861 ataagattat tctggacaaa aagaccaatc ttttctaata tgacaaaaag aaaataaata
    19921 ggagcataaa aatgaacgca ttaggtttta aattcccaat ccaagaacgt aaaacagcac
    19981 tctacaattt agaagaaaat tggtatcttc ctctacttgc tgaaaaaaga gggctgtctc
    20041 ttcaaaacat aaaacgcagt aattttggcc aatattcaat ggctgtaaac tatttaactt
    20101 ctgtattgga agaagcagca gccattaata aagttgcagt ttataacata gaccatatgg
    20161 cacaattata tttttatgga cctgatgttg taaccctgtt aaatcgtgct ttaaccggaa
    20221 attttaccga tatgaaagta ggccagtgta aatactcttt gctcttgaac gataagggtg
    20281 gagttcaaga tgatatgatt ttaatgaggg tttcagaaac cagtttcatt ttggttatca
    20341 atgctggaca tgatataact gatactgtag aaatcgataa taagaaacaa gaatatattg
    20401 ctgatataga tcggattatg gcttgcaaaa aaaaaggggg cgaagaagtt tttgctaaag
    20461 acatttcgga tactttagtg aaaatagata tccagggtcc actttcttat aaattgatta
    20521 aagaagtcta tggggcagat gtgcttaaaa atcgtaataa tcctgaaaag aacatgagtt
    20581 ttttcacttt caacgaattt gaattaaacg ggcatcatta ttatttatcc agaacaggtt
    20641 atactaatag atggggctgg gaactttata ttccagctgc agcagctgaa gaagatgcta
    20701 aaaagataat tactaaagct ctagattttg gcggactttt agttggttta ggtggacgag
    20761 atgaaaacag aatttcagct ggaccttttg gtctaccgct aatgggacaa gaatatgatc
    20821 cttatcatac acctctaaat gctccccttt ttgaaacagc agtagatatg aacaaggatt
    20881 actttgtagg aaaagatgcc ttagctaaag agattgcaga aggtaatcag aaacgacttt
    20941 atatttttgt ttctgaaggg attgtttcca acaggggaat ttataaagaa ggaaagcgtt
    21001 taggaactgt taccagcagc ataaattctc ctaatgtatc tttagaaatg cgtttagcta
    21061 ttggttccaa gcgtaaaaat gtaaatgaag aaaatggaac tgctgccata ggtttaggtt
    21121 ggttttatgc tccccttttt gaaaaagaca tcgaagggaa agaaatagca gaaattgaag
    21181 gtaagccaat tcgtatacca gtagaatttt atcgtgaaga tgaaaatcgt aacccgatag
    21241 gaagccctgt tttaggattt gttaccttgg aaggaataac tcctgccaca gctcataaag
    21301 cactaaaaaa catagaaaac ctgtaaatct acaaaaatgt aaacattaga aaacaagaaa
    21361 ttaagctttt gtaaaagata acaaagttca aagcatcaat ggctgaataa catttatccc
    21421 gcctattatt cattaggcag cagggattca ttccgttaaa attaattatg ttttagccaa
    21481 gatagctctt ttgcaagtgt attattaaga ggaaagttga ttgtttgggg tttatctttt
    21541 cacttgcaga gaattaatgt ttatgctctc tgttattggc aatcagctca taaagcaggt
    21601 atccatttag catataaaga accttatcaa aatccctgtc tccgggaata ataacatccg
    21661 cattcttttt tgtgggttca ataaatgctt catgggaggg ttttacagtg cttaaatatt
    21721 ggtctaatac attttcaaca tctcttcctc tttctacaat atctctttgc attcttcttg
    21781 ctaagcgtaa atcagaatcg gtatccacat agattttcag atccgatata gatataagtt
    21841 caggatagta aagagcaaaa ataccttcta aaataataag atctgcaaaa gcaacacagg
    21901 ttttcccttc ttttcggcaa tgcgtaacaa aatcataatc tggaatatca actgatttcc
    21961 cttcaagcat aagttctaga tcggaaagta aaaaatcaac atcgatggca tcaggatgat
    22021 caaaattaac tttcattcgt tgctgaaaag gcagattact taggtctcta taatagttat
    22081 cctgagccac gataactgtt tttagatctt ttaaacgcat accaattgct ttagcaatag
    22141 tagttttacc agaacaggtt ccccctccaa ttaaaattaa acgaacatta ttgagtggta
    22201 acatcttttt tgatccaggt atttattctt tctaacaaat tcctctcgat ttcagctgct
    22261 tgaaaatttg accaacaagc attaaagaag ttatcagagt attcatttag tttattgatt
    22321 ccgcgtaaaa gctccaatat cttatcagac acttctgtaa aacaatctcc ctgcaagtag
    22381 caattttcct caacaggaaa attctgaatc gctatctttg tgcaaactaa tgctttatga
    22441 tgtgctgcac aggaaagaat tctgccttca gtatcaggtt tttctaaagg tagaataata
    22501 aacaatgctt gttccaatat gttgtccaaa tcatcataag gagcataaga tatccttcca
    22561 ctgcaaaatt cctgcatttt ttcttctcca caaaggatga tacctatatt cttttctacc
    22621 agctttttcg aaatacgagg ataaatttcg gatacaatat ttttagctac tgtaagatta
    22681 tctttatttt ctggtgaacc ccaaaatatg atatatttct tatccttttc tgtgctttca
    22741 gaaaacctta aatcagttat aggtaaagga acaaagtggc tgttgctctt gttaatttta
    22801 tagaaattac aaagaagatc taaatctcca ggattggcat aaagacaatt tggcttttta
    22861 ttaatcaagt acttatccca aatatactgt tgaatatatt cccaaagata ggaaatatta
    22921 ttaagttctc tattgctttc ccatttttct ttcaaatcgg atatatacac tttctctaca
    22981 tctataatca tttcacaatt aggcaaaacc ctattagcta tcttcagtaa aggtaaacaa
    23041 gccaatcctc cgaaaaagat gatctcatat ctcttgctat ctagaatatg agcaaaacgt
    23101 cctgcttgac ctttacccag atgatgatag ataattggtt ccagtttgtt ttcagatttt
    23161 aaactcaatg aatatagttt gtcataaccg ctatgggaag agatgggctt ttctctataa
    23221 atatcacttt ttaaaagaag aaggtcagca tcaaaattcc gggaaacaat atcccataaa
    23281 aaacgagagc gttttgctct taatccctct tctattgcaa agggagtatc aataaacagt
    23341 actttttttg aaatcgtagc catcttatct tatatatctc ctattccttt aattttgaaa
    23401 tagcaaacta tgtcaagcac caaatggatt gagaacttaa ttgaggcgaa gatatcgtta
    23461 tataaagaag atagatatcc taaaaaaaag tactgagtat ttgtataatt tgtacaagct
    23521 gcaaaatcct ctttccaagt gtattttcat ttgtgtgaaa agctattttt tttatggtag
    23581 cataatctaa agaacggatg cgggaaaagt atttttatct tgacaaaaaa ttagcagtct
    23641 aattttgtgt ttgctaaata gaccataatc acttatatta ttaatgaaca agaaagataa
    23701 ttaatggagg ttaaaatgaa actcagacct gttgaagaca atgtggtagt taagcttaac
    23761 accgcagata atgaaaagac cattggcgga attataattc ccgataccgc taaagaaaaa
    23821 cctcagattg ctgaagtgat tgctgttggc aatgatgagg atttacaaaa aatcgtgaag
    23881 gtgggagaca aagttctctt tggcaaatat gctggtaccg aaattgaatt tgagggagag
    23941 aagcttttaa tcctttccaa aagtgatctt ctggctatag tagagtaata aaaatgtcta
    24001 tagtagcagt aaattcttcc acctttgaag aggaagttct aaaatcctca cttccagtat
    24061 tagtggattt ttgggctccc tggtgtggac cctgtaaagc acttagtcct actgtcgaca
    24121 aggttgctaa tgaaaccgca ggaaaggtta aagtagtaaa agtgaatgtg gatgaagctc
    24181 aggatattgc agcaaaatat tccattatgt ccattccgac cctgcttgtc tttgttaatg
    24241 gtgtagttag cgatcagctt ataggtttgg tacaaaaaga caaaattatg gaaaagctaa
    24301 ataagtttat gcaatagttt tgccctgaaa ctatagttaa agcccggtat gtaacaagca
    24361 ttccgggctt ttttaatttt gggaaaatat ggctttgtta aaatggggaa aaggttgtcc
    24421 ttgcttagtt agttgaggtg gagctaccgg ggcgcttttc gggaaattcc caagaagtac
    24481 cattgcatct acacggtagg taattatgga agaacaaacc ttttttcggg ataaaagtgt
    24541 ggaataataa aagagcggaa ggcaatatat cttccgctct ttcaattttt ggagaaagtg
    24601 aaggttattc gaatttcaac acgatgaatc ggggttgttt attggtatcc aaaacataca
    24661 ggcggatagt tttccgattt tctttttcca tattagtttt agctttttcc agctcagtat
    24721 tgaattcttt ggggctattc acttctttat cttcaatttg taatattaca tatccaactt
    24781 tcaatccgga tttggctgct ggtgaatttg gatcaacttt tgtaacaaca actcccttat
    24841 cactagtaac tcctaaacgc ttagctgttt gactatcaat agcttcaacg cttattccag
    24901 tagcgattcc accctttaca ctatcggagg cagcggcaat atcttcaggg tatgcttcta
    24961 aggtaacgcg gatagtcata tcttttttat cgcgcactat ttttaagggt atttgctgtc
    25021 ccaccctggc tgtggcaata gcaatgcgga atttgggaac actggggact ttttctccgt
    25081 ttatttcgat aattatatct ccggttttaa tcccggcttt atctgcagga gagtcttttt
    25141 ctactttggc tactaaaact ccggcaactt cttttaagcc gaaagcttcc ataatatcgg
    25201 gggttatttc ttgaggtaag ataccaatat aggcacgggt aacttttccg ctggcgacaa
    25261 gatcttcaac aactctctta gccaggttta taggaatggc aaaaccgatt ccaacattgc
    25321 ctccattagt acttgccagg gcagaattaa taccgataac ttcaccttta atgttcaata
    25381 aagggccacc actgtttcca ctattgatag ctgcatcagt ttggataaaa tcctgatata
    25441 taggagaatt atctcccaaa ttaagattag aacggctggt ggcagatatt acacctaatg
    25501 ttacagttct atccaatcct tcgctgaaag gatttccaat agcaatagcc cattcaccaa
    25561 tttgtaattt atcggaatct cctaaaggag caaccgttac cttttcgcct tcttttacta
    25621 caattttaat tacggctaca tcagtgctgg gatctaaacc tacgatagaa gcattataga
    25681 caaccttatc agccatcgta acagtgattg tgccatcccg acctttttct acaacatggt
    25741 tattggttaa aataaaagct tcacgggagc ctggattata ttcgtaaata aaaccactac
    25801 ctaaagaaac aataggacgg gtttgttctt gggggaatcg gaagaattgg cgaaaccaag
    25861 gatcgtctaa aaaaggatta atgtaatttt gaactgttac cttggcttca actctgattt
    25921 ggacaactgc ttcgcgtaca ttttttacta cttctactac aggattgggc ccattaggat
    25981 caatcatatt atctttggca ctagcacaac tcgtcatcag taatgctatt agcaccagga
    26041 ctaattgtct ccacattttc atagttatat ctccttactt tttaagataa gattttctct
    26101 aagtaatact tggaaaagat aaaaaacctg cgggtaaacc cgttaataaa atttatatat
    26161 atacattata atctgaaaat ggtttacggt caagtggaat ttgctactta ccaagattgc
    26221 catatttcct ttcgttaaaa aatactccaa tcactaccgt aaatcttaat aataagcata
    26281 attgcgaaaa agttaaagtg atttaggatg attgtttagg aaattcgctt ttcccacaaa
    26341 gccaaaatcc tgcttgagga gcagcatttt taacctgttt tgccattaat tgagataact
    26401 ctctaatttc ccattgggca ttttcatcac tacgcaaacg gacaaaatgg taaatttccc
    26461 ttaaattcat tttggccaag atcgtaacaa gggaggcgtt agttctaaaa tagggagata
    26521 aatatgccag ttttggaggc agcgaacttc ctaagcgaat taactgtgta ttgaggtctg
    26581 accatatttt gtctcgatta attttcttga tactaggagg gatttttgta gctaaagcgc
    26641 ttcctccctt ttgtataatt gtgcaataac gatgacgctt aaattgagcc cagcaacttt
    26701 cgctcattgt tatttcaaaa ctaaaatcga taagctcaaa agctcgtgga actttgtgcc
    26761 agggtttcaa attggtaaat acttgattcc agagctgtcg ttttataatt ttgggaagat
    26821 gggcaactgt ttctttagtt tcattccagc ttagttctcc ctgttgataa ataatagcgg
    26881 ctaaaacagt atcatcgata ttattgggag tgtggattat attcagttta ttttccatat
    26941 ccagttcttt cacccaagga agttcctgtt gcaaaaaatc ggtaaaacct actttctcta
    27001 aatttactat gccactgaaa ttgtcatctt ctgtatagcg aatcaaagat ggagcaatgg
    27061 catagacaga tttaaaaagt aagtccttca gttcagctgc ttcttttaag gggcttttag
    27121 ataagcgtct tagcaaattt tccaaacttc gggaattaat agtcattccc atctgagttt
    27181 ttgtagaaag aggtaaaatg taacgcgcat cctctttggc caggtattct ctttgtctgt
    27241 ttttaagttc tggttttgtt tctttataca attcatttaa agcattaaaa caatattcat
    27301 attcctcaaa aagggtgtcc atcatctgct tatatggttg cttcagtttg ggacggttgg
    27361 caaattcttt ggggacaata taatccttcc caaaagttac atagcgctga gatttttctg
    27421 tgtaagaagc aattctactg cgttgtacta attcagttaa cgctctggaa attcctaaga
    27481 tatcaatatt aaatacagcg tgttcagcta cggaactgtg tcccatttca aagataatat
    27541 tagtgttagt tttacgcgct ttttctaatt ctgtaatagc ttctttacgc agatcttcaa
    27601 tactcttatt gctgcggctg attctggcat aagctgcaga aatcaattct ggtgtagcta
    27661 cggggctatt taatttgtct ataagtccct tatctatatt atatccggcg acattaacct
    27721 tcatttgatg taacctttat tgtaatatgg caacaacatt ggaagcgcca attctattgg
    27781 ctcctgcttt aatcattgct aaagcttgtt cttttgttcg aattccacct gctgctttaa
    27841 cgccaatttt gggtccaact acttcacgca tcaaagctat gttttctatg gtagcaccct
    27901 gagagccgaa accggtagaa gttttaataa aatctgcgcc agctttcttg gctaacagtg
    27961 cacttataat tatttgctct tcagttagat agcaggtctc taaaattact ttaagcagag
    28021 cattatgctt attacatata ttaaccactt cttgggtgga aagcaaattg ttcttgtaat
    28081 caccactcaa taaagcgctt aaattctgaa ccatatcaat ttcttcagct ttagcttcta
    28141 aaactgctat agcttcgttt aaaatagctt ctggtgagga tgctcctaag ggaaaattga
    28201 taacggtgca gattttaaca ggttgttcca ggagttcttt tactaaagga ataaaatatg
    28261 aatttacaca aacagaagcg gttttatata aatttgctgt tttgcacaat tcttccacat
    28321 cttttcgttt tgcctctgcc tttagaatag tggcatcaat tacagaagct aaatttgtat
    28381 caggatcgca gatgcgctcc gggcttttta aatggcaatt ataacagttc agacatattt
    28441 tatgtccgcc tccacattgt gctaaatgat tatctccaaa tagatctttg gcaatttgag
    28501 gaatgtcttt catatcttcg ctccaagatt atttttatta agctgtccac aggcaccaaa
    28561 aatatctgca cctctgcttt tgcgtaaagt tattgcttgt ggaagggatt gagctctata
    28621 cataaaatca ttaatttctt ttactgtagg agctctaaaa atagaatcct ttccaggatt
    28681 ataaggaatg aaattaattt tgcaggagag atctccaaca tatttactta gtgcttttag
    28741 atcgtcgtca tccatattga aactgggaat caagatatat tcaatagtga tgcgataaga
    28801 acttttcctt agataataga gcaatgcttg ttttaattca attaggttat attgtttatt
    28861 tacaggcatt aatttactgc gttttgaatc tatggcagaa gttaaagaaa gagcaagttt
    28921 agcttttata ccactatcgg ctaaagctat aatgcctgga acaactccgg aagtggaaac
    28981 ggtgattcgt cttggactaa aagaaaggcc ttcttggctt tgtaaaattc tcagggcgga
    29041 taatacattg tctaagttgt ccaatggttc gcccataccc attagaacta agtttgtaag
    29101 tttcttattg tctgaacttt tcagttgcct ggctgcataa ataacctgac ctactatctc
    29161 ataattatat aaattgcgaa ttagtcccat tttggctgta gaacaaaatt ggcaattgcg
    29221 tccacaacca acttgggtag aaatgcagag agtatttttt ttctctgtag gaatcagcac
    29281 actctctata agttcattgt cttctaattt taaacggtat tttattgctt tatcgctgga
    29341 tacaagtttg ctatctgttt ccggtaaata gaaggagtaa ttatttaata aatatgtctt
    29401 aaactctgca ggcagattac tcatcttatc aggttcattg atgtaggatt tatacagcca
    29461 gaaaagaagt tgcttagttc tatacacagg tgagccgagc tgctctattt taattttatt
    29521 agccagctct tcaggcagta aagaatagat attggtaaac attattccaa aataggtaag
    29581 ctgataatgg gtaatttgga aaattcgtcc aaatcaattt cttggatgat tccttcttca
    29641 ctggcgagca taattttttt cccaataaca ttaagacgaa aaaccatcat ctttgtattc
    29701 tgatatgaaa caaaagttcc aattaaaggg aatcccttta cttcttctac ataaaaatcc
    29761 tcttcgaaat taaggcagca gagtaaacgg ccacagggtc cagaaatttt ggttaaatta
    29821 cctgctaaat tttggtcttt taccattttg atggttacct gattgaagcg ttttaaaaaa
    29881 ctaccgcaac aatattgttt tccacacata ccaaaaccac ctaaacgacg agcttcatct
    29941 ctacctgatg tttgatgtaa ttcgatgcga gttttaaaaa ctgtagctag ttcccgtaca
    30001 aaagttctga aatcaattct cccttcagca gtgaagaaaa aggttagttt atttccatca
    30061 aactgataaa ttgtttcaat aagtttcatt tcgaaaggat aacgctccag tatcgataga
    30121 aatgtccgag atgctttttc ctcttcataa cttaaatttc tcattttatc gatatcttca
    30181 gaactagcat gacggcgaat actgaaattt ttggaagtaa gaacttcagg agcaatttcg
    30241 tcttcttcta agcccaaatg aacaacctga gctatatcat cacctcgttc aacttctacg
    30301 atgattaggt cttcgggttg aatattaagt tcttcacgat ttcgataata acctatacga
    30361 ccggttctaa attctacttc tatatattgt tgcattataa cctcatatta tatatttggt
    30421 gataaagctc tctttctgta tctgtaaaaa ttagcttacg cctttgttta acaattgccg
    30481 ctgtttcaca aaaagcagaa aaacgatgct tatctaattc atctgcttgc ccccaagaaa
    30541 aatccggaac aaaatctcga atcaaattag ggccataaag attacatccg attccaataa
    30601 cggttcctgt attgatactg catccaatac cggttttact gtggtctcca atcatacaac
    30661 ccaaaaactg tgtggatgaa tctattttag ttttaagagg ataggaatag aaagccacat
    30721 ttttgtaagt atttttcaga tcactattat ttgtatcagc cccaagattt acccattcac
    30781 caatgtagga atgacctaga aaaccgtcat gttgtttatt gctatatgcc tgaaaaatag
    30841 ttccttccaa ttctccacct actttacaaa cgggaccgat agaagttcct ccgtaaattt
    30901 tagccccaat tttaattacg gatttcttgc ctatataaac aggtccacaa ataacagcat
    30961 tagccataat gcggacactg gtatcaatga tgatgggtcc ttcagaagca tctaaaacaa
    31021 cccctggaga aaattccgca ccttctgcaa cccaaatatt gtaaggatga agaacagtaa
    31081 ctccgggctc cgtttcaaaa taattatctc catcataaaa gtgcaaatga taatccctaa
    31141 caagtgatct ggaattatcc tgaattaaat cagcaagaga gtcatacagt ctaatattgc
    31201 cgagcttttt cttcagcttt aataaatctg gccaaactga agaatcatta acttttattg
    31261 caaccaggtc attacctgaa tataaacaat agcccatagg aagatcttta atctgagcaa
    31321 tagcttccac cgttacaatt atgcgtgaat tcaaatatag cttttcacca tttacaagag
    31381 aatttataat ccaagtagga tgatgttctt tatataaggg aacaagtgaa tcatctatta
    31441 tcacgcaagt tgtatctgta tcagaaaaaa tatgttccag acgctgacgg agtttagtta
    31501 ttccgcaacg gagatcacca atacttctgt ttaaagttaa tggaaaaaag ttagagcgcg
    31561 aagcatcgtc aaatattatg gtttgcatta ttattccttt tgctagtggg gcgttttcct
    31621 ttccaaacaa agatattaaa ctgtcctaag accatcgtta aaaccaagaa aaaattctgg
    31681 ataactaacc agataggata aaatctaagc atttccttgg taaatccaaa acgggaacga
    31741 gaataactta tagtgatgtt ttctacgata atccggtaaa gaaaaataat cagagcaatc
    31801 ttgatattaa aaaacaacat tatcaatcca ccccaataca taaaagccat agaaagtagg
    31861 ataaaaaaga actccggatt gaacttcagc tgatatttca tattagaagc ccaacgactt
    31921 cgttgattta ttagttgttt ccaggaagta acaggatgag tatatacaaa actggaaggt
    31981 agaaaattga aaattatttt gtgcccctta ttgcgcatta attgtaagag attcacatca
    32041 tcaccggaaa gcaaatgttt tattttagta aagccaccta catcatcaaa agctgttctt
    32101 ctataggcaa gattttgccc aatacaagcc cattttttac caattgtgta accaccagcc
    32161 aaaaccatat aagttaaagc aaaatcaaga tgttcatatt tctgcacaag tgatgccttt
    32221 ttccaatctg gaatataagt tctggaatat cctgccacca tagaaacatc atccgtaaac
    32281 atagaaacca tagaagaaat ccaggtttca gggacaaggc aatcagcatc cgtagtaaga
    32341 ataatttctc cttgcgttgc atatactgca tcttccatag ctttcttttt aggtgaaata
    32401 acattctccc tgcccagaac tttcaagtaa tgtagattta aaccggcatc aatatatttt
    32461 ttggctactt gctctgtatc atcttcagaa tcatcatctg ctaaaataac ttcaaatagt
    32521 tctttagggt aatcctgatt aagcaaacac agtaacaact cctgtaaatt tttggcttca
    32581 ttgcgagctg cgataacgac actaacagtt ctttttttat aattgaaggt gggtttataa
    32641 gttctatagc cattccaaaa acggatacaa aacacgaagt aggttatgca tcctgttaat
    32701 attaaagcac taaggatata aaaaataacg ctgctcatct attagtccaa tttacttttc
    32761 gttccccaac gggttggata aaaattatag cttaaagtat cagacctagc agagggaata
    32821 ttgtcctcta taaaatactc ctcgatacca gtatctgaaa gaaaaccggt tttaggattt
    32881 attgtccgtt tcacaattct atcgggaaca acaaaactat agcgtggatc atcatttcga
    32941 ggggtacgcc ctttattatc aattcgtatt gccttagtca taatttgtcc ccaaatagga
    33001 gcacaacttg cacttgcaga aattgctgca ttattgtcaa aaccattcca aattcctaaa
    33061 gtgtattttt tgttaaaacc aataaaccag gcgtcacgat ggtcactgga agtacctgtt
    33121 tttccagctg cctgccaata ataattattt ctggcagcag ctcccgttcc agaagtgaca
    33181 actgattgaa gcatagaagt cattaaaaat gctacttcgg ggctggtaac ctgatattta
    33241 gaagtagcac tacgttctaa tacttttcca ttcatatctt ccactctggt tataaatatt
    33301 ggtttagttc gatatcctcc attgggaaaa gtcgtaaaag cagaaattag gtttaatgga
    33361 gtaacttcat atgcacccaa agctgcagaa taatctgctg ctttagtatt tattccgaaa
    33421 cggagaaaag catcattaac aacatcaagt ccaatgtcca taacagcttt cactgcccaa
    33481 acattatagg aataagttat agcagttcgc attctggttg gtccataata ttgacggcta
    33541 aaattttgtg gagtccattc cttaccatct cctccggtaa gagtaattgg agaatctatt
    33601 ataacagtgg caggtgtata acctttttcc aatgcagcag tataataggt tggtttaata
    33661 gagcttcccg gttgacgctt agcttgagta atacgattat atttgctatg cgtaaaattt
    33721 ctacctccaa tcatcgctaa aacatgtcct gtattattat ccatcaacaa taatcctcct
    33781 tgtagataac gagtattaat atccacactg tttttagaaa cattgctgta acgcatagca
    33841 ggaggatagg attgttcgat tttagaaaga tagcggttta aaacagaatc tgcataaaca
    33901 gagagttctg catccaaggg agtgtaaatt cgtaatccac cttcaaaaag tttttcggtg
    33961 ccatatttac gttcaagata caaacggata tgttcaatat aataatcggt tgaatattgt
    34021 tgcatagaac tacgatcact agagattttg gaagcaactg cttcgttata ttcctcctga
    34081 gtgatcttct tggcttttaa catcttaccc aatatcatat tgcgtctttc aatcaaacgc
    34141 tccggatgtt ttacaggact ataataatta ggacgttgta tcatacctat taaagctgct
    34201 gattcagcta agttaaggtc tcgagcatgc tttccgaaat aatataggga agcagtttcc
    34261 actccgtgaa cctgacctcc ccagaagatt ttattgaagt agatctctag tatttcgtcc
    34321 ttggtgaatt ctcgttctat tcttaaagct aaaataatct ccttcatctt acgcgaaaca
    34381 cgtttatcca aagtaagaaa catatttcgt gccatctgtt gagttatagt gcttgccccc
    34441 tgagaaaaat ccattctggc aatatctatc actaaggcac gaaaattgcc tatgagatca
    34501 attccgaaat gatgataaaa atgcttatct tcagtaatta ttagtgcatc tataagatag
    34561 ggaggaagtt cttttaaaga aacaagtttg cgtttttcaa aagcaaacag atagaccatt
    34621 ctaccattac ggtcataaac ctcagatcct gaacgcaaag tgtaatttcg caattcactg
    34681 gttggaggta agctatcacg gtaaaaccat agagcaccta caaatatacc tgtgaatatg
    34741 caaaatgcaa accacaagta tttaagaatt ttatgtgctt tttcttgagt atgggcattc
    34801 attcttttat aatttcagag ccttcgtttt ttctatctat atataaagtt aggtaggcaa
    34861 ttatgctacc actaaccaaa gcaattgaga gtaggattgg tgtcaacaca aaaactctgc
    34921 tactatggat caggatggca taaactattg ttaactgcat aagattgtga gcaatagctc
    34981 cacatatact gattccgtat attcctaaac ctaaatgtag ccagcgaaca aaaaccataa
    35041 cgattatggc tgccatgcct gcagttaaag aaataagaat agatggacta agtaaagtaa
    35101 gggttgcaat tccgcttata attgttttgc ttatatttac tacgaaggct tccaggattt
    35161 tgttatgata gattagatag agaatcacaa tatttgaaaa accaacgcga ataaaaggta
    35221 aaggtaacat tcgcataatc agatcttcca caatatgaat agtggctgca attgcagtaa
    35281 aaaaagccaa ctggataagg gtagatttat tgtctatatt catttaagat attgttgtac
    35341 gaagctatcg gtggaattat aaagctgttt cacactacca ctaaatataa ctttgccttc
    35401 atttaaaaac aaaatttggt tgccgatagc atcgatattt tttaattcat gggtgatagt
    35461 tattaaagta gcggatgcgg tttccacaat gcgggttata taatacataa tttctcgaga
    35521 agtgatagga tcaagtccag aaacaggttc atcgaaaata atataatcag gatcgtaaac
    35581 taatgctctg gcaatactta tccgtttacg cattcctcca cttaactgag ctggatataa
    35641 actaatagta tcttctaaac caactatctg caaacaatgt aataccttat ctctaacata
    35701 agcgatattt ttttctccgc gttcatatag aggtagagct acattttgta gcacagtgaa
    35761 agaatccaac aatgcagaat tttggaaaac catggcaaag cgtttttttg tggctttttg
    35821 ttgttcttca tctgctctgt gaatatctat accatcaata ataacatttc cggaagcagg
    35881 ataatagatt cccagtaggg tttttattag aactgtttta ccactgccgc tgcgtcctaa
    35941 aataactaaa ttgttactat cttccagttt gaaattgatg ttggatagta tttctctctc
    36001 gttaagagaa acatataact tattaacttc gatcatactt acgcagatat aacaaaaagc
    36061 cgataattaa tccggaagca ttggctaaaa aatcgtagat tgatacatcg cgacctggaa
    36121 tatatctctg atggtattca tctatacctg caagtacaat aagtccaaga tagataagga
    36181 caatcttctt tatatcccaa tttttaaaac gcagccaata accaataaga catccaagta
    36241 cagcataaac gcctaaatgt tgcaatttat caaaaccgac aatgtttaca gaaggcaagt
    36301 cttgagaggg tatagatgat agtatccaga taattatcaa ccagaaaaga gtggggtata
    36361 tagttcggaa aaacttcatt tttttatatt acacagttcc caagtggaag aaatcccttt
    36421 ctttttaaaa gtggctttca ctgcaaactg acggttataa acccataaat tcaatttgtt
    36481 attttcttgt agcatattga atgtaaccat atcaatatag ggcatcttct tattggtcat
    36541 attctggaaa gatatctcat agcgaactgc aggatattta ccaagaggag tatcaactat
    36601 ttcagttgaa tagcgagtta tatttgcctg ccaggaaaca ccattagcat ctataggata
    36661 agttcctgta tctacttgtc cggaaataac ttttgccaga aaggaataaa tatcctggct
    36721 atttttagtt atagaatact gaagggtact tttgtccgaa ttacgataca taacagcctg
    36781 tgcagtatta tgattgtaag tagtggtaac ttcatcttga acatttttct ggtgaataat
    36841 tcgggtgtag gttaagggct gataagaacc attatgttta acgcgataaa cattgttaat
    36901 ttgaggaaaa atagatgatt ccctcacaga ttttgcccta atctcaacat agttatcagc
    36961 tttattttgt tgaatactga tttgggcaat tttaatccca acagctttca tataataatc
    37021 tgtggttatg gccgctaaaa atccattaac aagcattaat agtgttagga tatatttcag
    37081 gtcaattctc cggttttgat tttaacttga atctgtttta tgctcttctc atcagcatct
    37141 aaaatagtaa attcaattct ctcatcgtaa aatacagtat caccaatatt gggaacatga
    37201 ttatagtatg ccaatagaaa ttcagcaaga ttatcatagt cctccagaga aatatcagta
    37261 gagaaattct cattgaattg acggatgcta aagtttccgc tcaaaatgta agtgttttca
    37321 tctattttaa tcaattcggg aatctcttcc ttatcatact catctctaat ttcaccaact
    37381 atttcttcca gaatgtcttc caaagaaatt atcccgcttg tacctccata ttcatcaact
    37441 acaatggcta cctgcaattt tctctgtttg aattgattta acagggtttg cactttcata
    37501 ttttctgtaa cgaaccaaac tggacgcatc aattgcttta tggttttctt ttccggatag
    37561 agaatcaagt cttttacata tattatccca acaatttcat ctatactacc tctataaaca
    37621 gggattcgag aataaccgct ttctatgatt atctttttta ggtcttctat actttggttt
    37681 tcttcaatag cggtaatttt aacacggggg acatatattt ccttaatttc tgcttctctg
    37741 aatctgaata agccaacaag cattttcttt tcgtgttctt ctaaactgtg actgctgctt
    37801 tcagattgaa taagattgtg aaattcttca tcagtaaatt tagttcctac atagcggtct
    37861 acattttggt gagaagatat caactcactc atttttacta gcacccaaac gataggatag
    37921 aaaagatagc ggaacataag caatggtaaa gaaagtatac cacttaaagt atcagctttg
    37981 gatagagcaa tcaatttcgg ggaaatttct ccaaaaaaca aaatcaggat ggtcgtgaga
    38041 ataatttgaa tagtaaccac ggacgattct gtatagctat atttcctgcc aatttgtaaa
    38101 gccagtagtg tcccaaaaga ggatatagcc aaattaacaa aagtattgcc taaaagcaag
    38161 gtaattagca attctcttgg tttttttagt aacttaagga ttctttttgc gcttttgctt
    38221 ttactgtttt ccaagcgttt aagataaacc ctggaaagcg aaaataaagc tgtttcgcta
    38281 ccggaaaaaa aagctgataa cgcaaaaagg acgataatta taaatgcctt accgtcatcc
    38341 acttctaatt ttcaccttgt gtaagtttaa aataatattc ttctttagct ctcatttttt
    38401 ctgcgtcagc actgcgaata tgatcataac caattaaatg caataagcca tgaatcaaaa
    38461 ccgtgcggta attatcttcg ataatattag aatccctttg ataaaaaagc tgatttatgt
    38521 caataataat atcgcaaaag cgtatcggct tatcggggaa accatcttct gtaaaagtaa
    38581 gatcactctc gcaaaaaggt aattctgcag ttacaaaact taaaatatcg gtagtttctg
    38641 ctaaaccacg ataacgtttg ttatagcgtt tcattgtgtt actatctact atctttacac
    38701 agatttgata acagtgatca ggatcttctt tagaacaaat aagttctgcg attttttcct
    38761 gttccgattc gggaataata acagcaccta aaacttctaa actattcatc tttaggaggt
    38821 tccggataat caagacgttt acggtagata cttgttaaaa ctggaataaa acactgtttt
    38881 acggtttgca gatctttcat agaaataggt gcctcatcaa attgaccttc cttcagtaga
    38941 taatgtattg tatcatcaat aattttagca acttctgctt cattttcaat atttttggct
    39001 ttagtagtag attctacaat gtcagcaagc attactaaag ctgcttcttt ggtttgagga
    39061 cgaggtccag gataacgata ggtatcttta gacaattctt ttttctcttt actagctttc
    39121 tcataaaaat agcgaataac agaattgcca tgatgctgca taataatatc tatcaccggt
    39181 tgcgggattt tgtacttttt cgccaaagct atcccgtctg ttacatgatt acgaattaat
    39241 tgagtgcttt cttctggttc catttgcgaa tgaatatctg ccgaatcctc gttgttttcc
    39301 gtaaaaatat ctggattcgt aattttacct atatcatgat aataactgcc tactctggct
    39361 aatagtggat tagctccaat agcttcagca gaatgttctg ccaagcttcc aactaccaaa
    39421 gaatgatgat atgttccaac tgcttcagtt gctaatcttt ttagaagggg atgattaaaa
    39481 tctaggagct ccaatagtgt ctgttttgtt gcccgattcc atttatgttc ataatatgga
    39541 acaattaaca aaattccact aacgctaaga gttgaggata taaagctata acctgttgaa
    39601 cgcagtattg ttagcaaagt atctctttta taaatcccga tactgataat taccactatt
    39661 tgggataaaa gcaaatataa ccagatcatc aaatattcgt gccaggcatt ctgacggcgt
    39721 aaaattaata aacatattac cgaggataag atcataatga ccggagtaaa tgtttcccaa
    39781 ttcagaaaag gactgataac cagtatattg cagatgctat acaataaccc aaattcaaaa
    39841 ttaaccagaa tggctcctgc gataacaatc atagcaaagg gaattattaa attgtttgca
    39901 gaaagagcat aattactaag cacagtaaaa accactaaga ggataaaccc caagtttaca
    39961 ggtataaaat ctgccactgg tatcttttct tctttgcttt gaacaccaaa atagtgattt
    40021 gtaagaaaaa tatttaaaaa gacaaacatc agcaaaccca aagtaagaaa catttcttga
    40081 aggggagatt ttcgtatttt ttgacgccga tatgcttcct gtaacgattc cagcttacga
    40141 acttcgtctt gagttattct ggagtttttg cgaactataa cctcattccg taaaaccata
    40201 cctttacttt ggtaaattgt tcccagagaa tcttgattca gttcatttag tttttcttca
    40261 tctacttgga ggttaggttt caggaattgc tcagaaaatt ccaaaacgaa ggttttagct
    40321 tccggaaaat tcgttaccag atcttcttta acctgttcta tagagagaaa ttctcttata
    40381 tttactgttt tcagctttcc attttggtca attaatacac tatcttcttt caatgtctca
    40441 tagatgcctt gtttgtaaat agctctaagt ttattacgca gagattcata tattttatca
    40501 cgaagcatag gatgtgtagc aaaactgagt ccttcagaag aaaaaatata tccatttcgt
    40561 ttcagctctg tggcaatagc atttggatct aaagttggtt gtaaagaaca taaaacagcg
    40621 taaatattat ctaatgcctt gatagcctca aacatcgggt catcagatat ttcataaggt
    40681 ttattaacct gagataaaac ttgttccctt tcatcggcta attcgctggt agattttaaa
    40741 ataggataat caaagggagc aattatctcc acatctgcta cctgaccttc cttcagatta
    40801 tattcagggt agtttactct gccaacggca actatctggt aaataccaac aattactata
    40861 gccgttacta tgcaaagaag aatgtatttc gaattcatat ttttatcttt tttatagagc
    40921 ttgttctttg tcaagtgtca tttaacataa gttcaacatc ctctctattc ataatacacc
    40981 cgtaattaat caataacctt gcattcagca aaatactcag ctatcttttt cacttcttcc
    41041 gggatattaa tatgtctggg gcacttggaa acacaagctc cacaagcaat acacttatct
    41101 gctttgcttt cgtctggaat gaaaagttca tattcttttt ggtgcctgtc tttttcatta
    41161 aacataattg aatcgttata tataccaaaa atatctggga ttgctacttt ttgagggcag
    41221 ggaagacagt atctgcattc agaacacata acaggtattc gtttcagaaa ttcttgccta
    41281 acttctttat atatttttag tgtcttagca tctaaaacat ttgctttaca aagattcgca
    41341 taatgcacat tttcttttac ctgttctaaa gaactcattc cacttagtag aacctgacag
    41401 ccctccaaat tccaaaccca tcttaaagca aattttacag ggcttaaacg gatattatgt
    41461 tttttccaaa cagctgctac attttctgga agatgtcctg caagttttcc tcctctaaga
    41521 ggttccatag caacaattcc agttccattt tgtcctgcca atttatagcc attcattcca
    41581 gcttggtaat ttgtatccag atagtttagt aaaaattggc aaaaatccca ttgataagca
    41641 agcaggattt ttttgaatgc aggatagcta tcgtgaaagg aaaaaccgag atgtcttact
    41701 tttccttcca tttttgcttt atctagaaaa tcaataaccc ccaatctttt catttccatc
    41761 caggactttt gatttaatga atgtagcaaa tagtaatcta tatagctggt ttgtaaatgt
    41821 tccatctgtt tttccagaaa gttatccata tcagctctat ttttaactaa ccaacagggt
    41881 agttttgtgg ctataaacac tttattgcgt tcaatctggc taagaaattt gcccaataag
    41941 ggttcacttt ctccattatg ataagaccaa gcggtatcat aataatttac accattttca
    42001 taagcataat gtaacatagc caatgcttgt tcttcgtcta tttttccttc tttatttttc
    42061 ggcaagcgca tgcagccaaa accaagagtg gatagtttat catccaaata aggcatttta
    42121 cggtatttca tttgttttcc tcattgtgaa agtgattata aattttggta gcagttttaa
    42181 ttcctattcc tttaatgctt gaaagggttt ctatatctgc tttggctata ttatctatgc
    42241 taccaagttc ttttagtagc aggaatttat ttgtttctgc aataccagga atagcttcca
    42301 aagcactttc cagagttctt tgagttcttc tttttctgtg aaaagttata gcaaaacgat
    42361 gtgcttcatc acgaatagta attaataatc gtagtgctga agaagatcgt ggaagaacta
    42421 tagattgttc atttccagag agatagacct cttcagctct tttcgctaaa gcgattaagc
    42481 tgatatcatt tttaccggaa gagttaagaa tttgttcact ggcagaaagc tgaccttttc
    42541 ctccatcaac gataatcaga tcaggtttca tttgttcatc tttttcagtt tctgttaaaa
    42601 aacgagccat cgtttcttgc attgctgcaa aatcattttg ggtgtctata ctccgaataa
    42661 tgaagtgacg gtagtatttt ttcttgcttt taccgttttc aaagaaaacc gcggatgaaa
    42721 cagtatcact tccttgaata gtagatatat ccatacagac aattttacgg ggcagcttgg
    42781 gaagttttaa gacttctttc aattcctgaa tcgggaaaat agtacggttg gctttacgca
    42841 aatgtgccaa tttactttct tctacaatgt taaaagcatt gcgtttagcc atagcaataa
    42901 gtttggtttt ttcgcctctt tgaggaacct gcaatttgtt tcccagccaa gaattaagtt
    42961 tatcaatatc tttgggagca aaaggaagaa gaacttcatc gggcatttct tctttatctg
    43021 catagtaaag ctgtaaaaaa cttgccagga tgctgtctga ttcttcataa tccagatttg
    43081 caaggggata attttcctga tggatgattg ctccattctg catcttaaaa actacacaaa
    43141 cagctctttt ttcttccctg taaaaaccaa taatatctgt atttccacct tcaacattaa
    43201 atacagattg gcgcttttga atactttgaa ttgccacgat tcgatcccgt aattttgccg
    43261 cttcttcaaa atctaaagaa tccgacgcat tttgcatttc agcgcgcaat tcatcaatca
    43321 attctgtata gcgtccactg aaaaaacgaa gtaagcgatt tacaataacc gcataatctt
    43381 tttggttaat gcatcctaca caaggagcag gacactttcc cagctgataa ttcatacatg
    43441 gttgtttgta ttttgtttta gggatatttc gattacagtt tcttaaagga aatatccatt
    43501 ccagatcgcg taaagttgag cgtagataac ggacttcagt atatggtcca aaatatttgg
    43561 catcatcttt caataattca cgagtaataa aaatgcgtgg gaaaggttca ttaactgtta
    43621 tttttacaaa gggatagcgt ttatcgtcct tcagcattat attgtatttg ggacggtgat
    43681 gcttgataag agttgcttct aaaataaagg catctgattc actgttagtg atgatatatt
    43741 caagatcagc aatgtttttc accagatgct cagtttttac atccttgccg cttgaagcaa
    43801 aataacttct tacccggtgt tttagattaa gagctttgcc tatatagatt atagtaccgt
    43861 ttttatcttt ccaaagataa actccaggtt gatctggtag caaagaaagt ttagattcta
    43921 tttccttaga aagcgcttgc atagtgcttt tcctgcctga gtgatataat caatttttat
    43981 taaaatagcg gataaataaa acaacccaaa ggcgactacg gaaaaaacgg catctctgat
    44041 taataattca attctaccct gaaccggaag tatattgcgt cctattacca ataaaaagaa
    44101 tataataagg caaatcagta aagttttagc tatgtttctg atgattccag aaaaagttat
    44161 acctggcatt ttctttctaa tcagatagag caaaataaaa taattcacac ccgcagtaat
    44221 agatgtggaa agagctaaac cgcgatgagc cataaattgc ataaggatga agtttaaagt
    44281 tatatttaaa gcaaccatag ctgcagctat ttttacaggg gttttagtat ctttatgggc
    44341 ataaaaaatt ggcgtaattg tttgattcaa accaaaaaag actaaaccca gagaataaaa
    44401 aataagtgct tgggaggtca tccaaacagc tctttcattg aaagcaccac tctgaaaaag
    44461 aattcttaca aaatcttcgc ctaatgctaa tataatagtt gtaacaggta acataatata
    44521 agcaagattt aaacctgtaa aacgaatact ctccgatagc tcatcatatt tcccattagt
    44581 tacgcagcga gaataaaaag gtaaaactgc tgttccagta gaaatcccaa atatacctaa
    44641 cggcaattgc attaaacggt taccaaaact taatgctgta attgatccga tgggtaaaaa
    44701 agaagccata agtgaatcgg ctatcagatt tatttcccga atacctattc ctatcatact
    44761 gggaattatg cgtttccaaa gctgagcaag agctgtagaa ccaaatctga aacaaaatgc
    44821 ccaacgatat cctatttttt ttaagtaagg aaaattgacg attgtttgta aaaaacctcc
    44881 tactatcact ccccagccag cccagataat taactcttct ccactcactt ttagaacata
    44941 ataaggaata atgatagtag atatcattcc aacattaagt aaagcggatg aaagacctgt
    45001 cataaagaaa tagttatgag aattgagaat ggcaatgaaa gtagaggata aaccaatgaa
    45061 aaacaaatag gggaaaatta tccgggttag ctttatagcc agaacagtag tttcaggagc
    45121 cagtccagga taaagacatt ttacaattaa agaggcaaaa gctatcccta agatagttag
    45181 aattacaagg ataagagtta aaatactaag caagtttaaa gcaaaatcta tctgtttatc
    45241 ttttccatct tcaattaaaa tctcattata gataggtacg aaagctgtag aaagagctcc
    45301 ctctccaaaa agtcgtcgaa gcaaattagg tatattataa ccaacattaa aagcatcatt
    45361 taaataggta gtaccaaaga aaaaagccat cacttgatca cgaatcagac ctaaaatacg
    45421 gctgaaaaaa acaccaatag acatcacact gatgtttttt gctaatgtcc gattcgagtg
    45481 ttgagattcc atttaagagt ttaagaggtg tttacagttc aagtacagat tggtcataac
    45541 caggttgaga tttacattac cctcaatttt acggttgaga tcttcaagaa acagtaaaaa
    45601 atcgtataga cgatcatcta taccagattt attttctacc attgctttca gatcatctat
    45661 tttatcaata ttggttattt ccgcttcaaa attagcatta agagctccta aatcactggc
    45721 aataatgcgg atatatttca gtaaatcaat tacctgatct ttcttttgtt gatccttata
    45781 tgcttttgct aatcctaaat agcccaaatc atctttggtt gctgctaagg caaaaatctc
    45841 aaaagcccaa ttgcgcgata atgaaacact gtctgaagca acccgaatag cagtttttaa
    45901 attgcctgca cttattcgag aagcagttct agcaattgga gcacttattt caaattctgt
    45961 agtaagaatt tcttcaataa cactgcgtgt gaggggtcta aaatatatag gttgagttcg
    46021 agaaagaatt gtaggaagaa gtttttgaat tcgttcagta ataagaatta taatagtccg
    46081 ttctggaggt tcttccaatg tctttagaaa tgcgttagcc gtagctatag tcatcatatc
    46141 tgcatcttcc acaattacaa ttctatatct tgcttcgtga atggatagtt caaaatgttt
    46201 gctaagcatt gtaatgcttt ctttgcgaat ttcaatagta tccttgaaga aaaaatcagc
    46261 ccagggagtg tttaccttat tctcaatgta cttttgaaac atttttaagg catcagagtt
    46321 tttaatctca ccttcaggac tgaggttcaa atttggagta gggaaaatat acacaaaatc
    46381 tggatgctcc agctgcagga atttatggca ggaatcacaa tgcccacagg gttttaattc
    46441 atttgttgcc atacaattta cagccatacc aaaatatagt gctgttgtaa atttgcccac
    46501 accatcactt ccataaaaaa gataagcctg ggatattcgc tcattttcta tagctttacg
    46561 caaaagcttg agagcgtctt cttgtccttt tattttgttt atcatttaca caatactaat
    46621 tttactgcga cctgcttcgt cactaacact gtaggtatat acctttttaa tataaggact
    46681 ggtgaattca ctggcaagca tatcgtaaat aatcacatca tgatattgac cggatacaaa
    46741 cataaattct ctttgcacac caaccttttt aaagccaatt ttttcataac acctgatagc
    46801 tcgtttgttg taagcaagaa cagaaagata tacattatgc atattcaaca tattaaatgc
    46861 aaaatcgagc aaaagattcg tagcttcaga acctattcct tgattccaaa aggtcttttc
    46921 cccaataaaa attcctaaag aggcattccg atgaacatca gaaacttgat gtataccgca
    46981 atttccaatc accttattag tatctttttc tacaattgca aaaatgcagt tattcttcat
    47041 caattcacgc aataactcac tttctttttc gatatctata acctgatgag agagggttac
    47101 aaattgtcct atttccatat cattcagcca ttcagtatat ttttccacat catccataga
    47161 tacaggagaa agataacatt tctcccctac aagtttacga aaatacttca ttttattctc
    47221 cctgtgttta tacatattat ggtatttata aacttcttat ggtattgatt tcttaacctt
    47281 agcaaagtgt caagaagttt ccgaaattct cgtttcacac caaaataaag atatgattgt
    47341 tccatatttt ggacaaggac aatgtctcgc taagatatgc cataattatt aacctaccct
    47401 taatatatgt ccagcagaac tagttgctac tcaacatcat ctaatagcat acaataaata
    47461 ttgttcacaa gcaacaactc atactcctat aaatgatatc attatgatga tctgtgcatt
    47521 tcttgcttga ttatctacaa tagagctaca ctgaagctat tcgggatttt gccaattaac
    47581 agcaccacaa ctccacgaca gctaactaag caatacataa ctggctaaaa cagtagaatg
    47641 ctgatttagt aaaaaggcaa taaaaatggt gattattttc ccagagaggc ttataaatct
    47701 gttttaataa aaaatatcac cataagttgt cgtaataaca aaactctctt gagggtgatc
    47761 catcataggt tttttgcatt tataaaacgg tataactgct actatagttg tattgtccaa
    47821 agacttaaag caatatctct atatttttat ggagcccaat taatggcatt aaccataaaa
    47881 atttatagag cataaggaga aatattgtaa acactttctt tccctcaact ctatattcta
    47941 tgtcaatttc ttttaggcga ttcactcttt ttttattaca gagacaaaat catctagatt
    48001 tattaggtaa aacaactcat tagtaagcga catatcatac ctagttcttt tctctcttat
    48061 agtttgtatt actttctctt attccaatcg aattattttg agcagtaaat atatgatata
    48121 ttacacagta cttgacagaa ggcaaggatc attttttttg gattttttac aatgaaaaat
    48181 ggaagcgaaa gtggcggaat tggcagacgc gctggactta ggatccagtg agttatactc
    48241 ataggggttc aagtcccctc tttcgcacca atttttttaa tatgataaac aggagtttcc
    48301 agtgcaagta gaatttaatc aaattaatgt tgtaatcaaa gaaattaaca ttacaatacc
    48361 ttcagacgaa gttacaaaag cttatgaaaa atatcttgtt aaaatagcca aggagataga
    48421 agttcctggt tttcgtaaag gtaaagctcc tctatatatg gtcgagcggg tgaatactga
    48481 aaaaataaaa gagaattttt gttatgatta tgttattgat gtatttgaca aggttgctga
    48541 agaaaaccac atagaatatt tactttatcc tgatataaaa gatctgcaat gggaaaaagg
    48601 caacgatatg acggttaaga tacaaattga acatgaaccc cagcttgagc ttaaacaaat
    48661 agagggttta accgttcctt ataaaccagc aatactggaa gaaagtgtag aaaaatttat
    48721 cgctgattta attgctcaaa acacaataat aaaagatgtt gaaatagctg aagaaaatga
    48781 tgaagtagaa gtggaactaa ttgttcagaa taacagtgat acttatactg tttctgctat
    48841 tttttatgct tgttcagaag ataatgatta ttcaatacct gcgctaattg ggcataaaat
    48901 tggagatact ctttccgtta tcttaactgg taaaaaaatt caaaccaatg cactgaatgc
    48961 tgattttaat cttgttgatg atactgaata ttcttgcgaa ctatgtatta atcggattag
    49021 cagaaaagaa attcctgctc ttgatgatga atttgccaaa gatatggaat acgattcatt
    49081 ggaagaaatg cgggcaaaaa tagctgaaga gatgaagttg agaattgagc atattaatat
    49141 agacatagaa aataacagta taattaataa attatttaca gataaccctt tccctttacc
    49201 tatgaaaact gttgaatatc tcgcatttca acaaatcagt gatgttagtg atgataacct
    49261 aaggaagctt ttactaaatc gttattatta tatgattatg aaccaaatgg tacaattata
    49321 tataggtaat gctcttatga aagaaaaacc tattgaattg accgatgaga tggttgagga
    49381 atatataaat catcaagcta ttatccaaga tgtaacggtt gaggcatata aagaacatag
    49441 taaaatagat ttttcctcgc aggacaccca gaacagtata aaagagtact ttattcttag
    49501 gcaaattgcc aaaacctgcg agtttgtgat agaaaaagaa cctccggaaa cagaaaatac
    49561 agaatccgga acagaaaaaa tagaggagga ataatgagct ttgtccctat cgtaatagaa
    49621 caagttggcc gttctgaacg tgcttatgac atttattccc gtttgctaaa agataggatc
    49681 gtttttgttg ggggagttat tgaagacaat ttggcaaata ctgtagtggc tcagttactc
    49741 catctggaag gagaagatcc tgaacgcgat atttatatgt atataaatag tcccggtggt
    49801 gttatttcag ctggtctggc catttatgat actatgcaat atattaaacc tcgggtaagt
    49861 actatttgta taggtcaagc tgcaagtatg gctgctgttt tacttgctgc tggtgctcct
    49921 gggaaaagaa ctgccctgcc aaatagtaga attatgattc atcaaccaat gggtggagct
    49981 cagggtcaag caactgatat tgaaattcaa gctaaagaaa tcctatattt acgtggacgg
    50041 atgaatgaaa ttttacacaa gcatagcggc caaactatag ataaaattag agaagataca
    50101 gaacgcaatt attttatgag tgctgaagag gcacaagaat acggtatcat tgacgaagtg
    50161 atatcgatgc gaaaatagct tgactaaatt ttagcaatct tattatcagg tataggtgat
    50221 atcaatatat cacctatgaa tttttaaaat attaaaagga ggaatccttg gagaaataca
    50281 gaacattaac ctgctcattc tgtggaagag cggaacatga agtaaaaaat ttaataagag
    50341 gaattgcagg taatatctgt gacgaatgta ttgttatgtg tcacactatt atagattcca
    50401 gtaaagagga aatttctcag gaagaatttg atgctcctgt tcccagtaga ataaaagctt
    50461 atcttgatca atatgttatc ggacaagata atgccaaaga gattatatca gtggcagtat
    50521 ataatcatta caaacgcata ttcaaacaga aatataaaga tgatatagat ctggaaaaaa
    50581 gcaatatctt gatgataggt cctaccggaa gtgggaaaac tcttattgct caaactttgg
    50641 caagattttt acaggttcct tttgctattt ctgatgccac aactttaact gaagcagggt
    50701 atgtgggtga agatgtagaa aatatacttg tccgcttatt acaaaatgca aattatgata
    50761 ttgctaaagc ggaacgagga attgtatata tagatgaaat tgataaaata agccgtaaat
    50821 ccgaaacgcc ttccatcaca agagatgtta gtggtgaagg agttcaacag gcattattga
    50881 agatcttgga aggaaccaaa gtgaatgtcc ctccaaaagg tggacgcaaa catcctcaac
    50941 aggattttat tgaaatggac acaaaaaaca tcctgttcat tgctggtgga gcttttttcg
    51001 gtttggagaa aattatcaag gaaagaatgg ataaaaaatc agtcggcttt gatgtagagc
    51061 tcggtaaaag acaggatgaa gctactattt ttgattatac taccccccaa gaccttttaa
    51121 agtatggttt aattcctgaa ctgattggca gaatgcctgt tatttgtact ttacatgaac
    51181 taagcgaaga agctttagta gatattttag tgaaacccaa aaatgcaatc tgtaaacaat
    51241 atcaaaagtt ctttgaaatg gatggagtcc agctggaatt tacggaacca gctttaaaag
    51301 aaattgccag aaccgctatc aaacaaaaaa caggagctcg cggtttgcgt tcaataatgg
    51361 aaaaatttat gctaaaaatt atgtttagta ttccggatag gactgatatt gaaaagtgta
    51421 taattactcc tgaggtaata actaattctc aggaacctcg atatgaatat atcaagccac
    51481 ttaattcgcg caataaagtt gcgaacgaaa aataacagga gaaccgataa tgacttctga
    51541 ggttactatt ccgcgccgtg aaaaattact ttccacttta aaggaaaaag aagcactaat
    51601 tatctttgca gcagaggaag caaaactgga aaaattcaag caggataaca acttcctgta
    51661 tttaactggt ttagacattc cggaagcaat ctattttgct ttcagaggac agaatggagc
    51721 tatagagatg ctgtttattc agcgtggaga tccagaaaaa gaaatttgga caggagcaaa
    51781 aatgacagct gaagaagcaa gccaaaaatg tggtcttagc cttcagaaaa tacactatct
    51841 ggatgagttc ttaagtattc tctctttcta ctgtccagta attgataaaa tttgggctaa
    51901 tatcggagag caatatttag ataaaccacc tacaatgcaa cttttccgtt tatctccaat
    51961 ccgcgaacgc tatcctaata ttattattga atccattgaa accataatta ctcccttgcg
    52021 aaaaataaag agcgactggg aaataaaaca gttacaacga gcaatcgata taactggtaa
    52081 agctataatt gatgttctgg agaaggcaga agccttgatg atggaatatg aactggaagc
    52141 aataatgttc tatcgaatgc aaagtatggg aattaaacac tggggttttg ctccaattat
    52201 tggaactgga attaatgctg caactttgca ttatgaaaaa aatgaatgcc agatagagaa
    52261 cggagatgta attctgatgg atgtgggagc ttcctattta aactacagtg cagatatcac
    52321 tcgttgcttt cctataaatg ggtcttttac tgatcgtcaa aaagaagtct atgaagtagt
    52381 tttggatgtt caaaaaaggg ttattgaaat gatcaaaccg ggcattgaac ttttagaact
    52441 aaatcaggta gctcgcgatc ttttagcgaa gggagcaatt gagcttggct taatagatag
    52501 agaagaagat atttataaat attatatgca tagcattagt cacttcttag gtatggatac
    52561 tcatgatgta ggtagccgaa atgctatttt agaaacagga aatgtgatca ctgttgaacc
    52621 gggaatctac attccggaag aaaaacttgg tatccgtatc gaagatgatg ttttagtaac
    52681 agatactggt tattgtgttc tttctcaaca tatccccaag gaaatttctg aaatagaaga
    52741 aatccgtatt aaggcaatga cgaaatgatg tctaaagcaa tttatccagg tacttttgat
    52801 ccgattactc tgggtcatat gaatattttg gaaaaggcct gcagtatttt tgacgaagtt
    52861 attcttgctg tggctgatta cactggtaag gataatcttt ttagtttaca ggaacgctac
    52921 caactctgta aaaaatctac agaacatatc cctaatgtat cagttagcac ttttcaagga
    52981 ttgtttgtag attacgctaa aagccaaaac tgtaatttaa tgattagagg aatgagagca
    53041 gtatccgatt ttgaatatga actatcttta gcgttaacca ataaaaaact caatcctgaa
    53101 atagagaccc tgtttttggt gccttcttta aagtatatgt atctttcttc atcaatcatc
    53161 cgtcagctgg ctgctttaca aggtccgttg gaagattatg ttcctacctg tgttgctgaa
    53221 gctatgaggg aaaaacactt tagttaatat tatggctaaa caagtcgatg ctgaaaaatt
    53281 gaacaaggta caaaccgaga gagctttacc tgctgatata aatgcagaag cagctcttct
    53341 ttcagctatg ttaatagata gcgatgttgt tagtaaaggt atcgaaaaga tcaaagagga
    53401 atacttttac cgcaatgcgc ataaaatgat ctttcgcaca atggaagagc ttttcaatga
    53461 aggcatcgaa atagatcaat taactttaat caaccgcttg gaaaggaata actttctgga
    53521 aaaagttggt ggtattccct atattaatga aattgccgat tttgtagttt ctgctgccaa
    53581 ttttgattat caccttaata ttgttactga gcaagcatta cttagacatt tgattgttgc
    53641 ctccaaggga attattgaaa attgctacac agctgccaaa ccggttaaaa caattgtcga
    53701 tgaagctgaa caggctattt tcgctatagc tgaattaccc agtcatcagg gttttgtgag
    53761 ttttgatcaa gttagccagg aagttattac taatatagat aaaatagcct ctaccaaaac
    53821 atctgttaca ggtttaagta gtggttttgg agatttagac cgttttactg gaggttttag
    53881 acctggtcag tttattatta ttgcggctcg tcctgctatg ggaaaaactt ctttggcttt
    53941 aaatattgca gctcatatag ctgtgaattt aaacaaaaaa gttgctattt tttctatgga
    54001 aatggcagcc gatgaagtag ttatgcgaat gtttagttcc gcctcggaag ttaaaatgga
    54061 tggtatgtta cgaggttacg gaatgaacga agaaaaactt atccgcatta tgcaggcatc
    54121 tgaagtgctc tcttcaaaac aaatattcat tgatgattct ggaacaaact caccattaga
    54181 tatcagagca aaaacaagaa gattgtcagc agaaattggt ggtctcgatc ttattgtaat
    54241 agattacttg caattaatgt ctttagtaag ggaaaaagat aaccgacaac aggaaatctc
    54301 agaaatttca cgcgctctga aaattctggc taaagatatg aaaattccag taatagctct
    54361 ttcacaatta aaccgcgcag ctgaaacccg tgataataaa aggcctaaat tagcagacct
    54421 gagagaatcg ggagctattg aacaagatgc tgatattgta atgtttattt atcgggatga
    54481 atattatttt gcagaaacaa ctaccaaacc gggaatagcg gaaattaata tagcaaaaaa
    54541 tagacacgga cctactggaa gtgtagaatt aggattcgat caggaattta ctcttttccg
    54601 gcctctagaa acaaaatatg atcagtaata tctttgccgg cataggtgaa tacacaatct
    54661 ttgtcggaaa gtgcctatct aaaataactt ctctttttaa gcgttatcag gaattcttga
    54721 ttcaattcaa acgaattggt ttcgattcgc tatttctgat ttttttaaca gctgctttta
    54781 caggactcgt aactgcttta caggcagtat atcaatccaa aggttatgtt ccagtaaatc
    54841 tacttagtgt gttgattggt aaatccacaa tgattgaatt agcgccagta ttaactgctt
    54901 tagttttaac cgggaaagtt ggtgcttcca ttgcagctga aattggcacg atgagagtaa
    54961 gcgaacaaat tgatgctttg gaaagtatga atattgatcc ttacgaattt ctctatatgc
    55021 caagaattgt tgcaggcatc ttagcctttc ctcttgttac tgtttttgct aatgctgtag
    55081 gtatagtttg tgcctggtat ttttcctggt tacattatgg acttcattat tacacttttt
    55141 tcaataatat gcgcagttat tttgtaactt ccgatctttg gggtggcata gccaaatcga
    55201 ttgttttcgg atttattatc actagttttt cttgttatca tggagaccgt tgttttgggg
    55261 gagcagaagg agttgggaaa gcaacaacat taactgttgt ttattcttcc gtttttattc
    55321 tggtgatgga ttttattgtt gcctgggttc tgtttggagc atattaaaat gaaaagttgg
    55381 aaacttagtt tactgttagc tcttttgctc ttattttcat gtgggcagga aaaaaataag
    55441 cagggaaaca cacattacaa acataaactc gtagtctaca ctaccgatga atttcgtaaa
    55501 tcgggttttg aacattctat agttcctgat tttcaaaaca agcataaatg ccagctggaa
    55561 tttgtgcttt ttcgtaatac tgcagagctt tgcaaagcaa ttaaagataa agcaaactat
    55621 ggcaaatacg atcttgctat tggtattgat aatagctttg cagtttcaga aacgctggct
    55681 gttaattttg tgcctccaga atcttttgat tcagaattgc taattcggga aactatcttt
    55741 gatcccgcgt taagattaat accttatgct tatagtaatc tttccctgat atataatact
    55801 acccagataa aaaatcctcc tcaatcattt ggagaattgc aggacgccaa atatcttgct
    55861 caaattgcta tctgcgatcc tatcagttca ggtttgggac gctcaaccct tttttggagt
    55921 gtagcacttt ttggatctga gggctatgaa tatttatgga agagcttacg caagaatata
    55981 tacaaaactt atgctactca tacagaagct ttagaagcac ttaaaagagg ggaatgttca
    56041 ttaatgattg gatacaatac cactcctgcc tttttacagg aacttgatcc cttaaacaaa
    56101 gatttccagg tttctatgct aaaagagggt agttatcaat atatagaatc aattggtatt
    56161 catcgtggaa cttcaaaatc cgctctatgc aataagtttg ttgattattt tctggcttcc
    56221 gaagaacaaa aaatggtaat ttataagctg ggaatgttcc ctgccaatag aaagaccctt
    56281 ctgcccatgc atttttcagc tatacccttt ataagctata gtgtcaacga tcgcatatcc
    56341 aataatttga ttcaagaaca aattttgtca tggctggatt tttggcaaag gttatttggt
    56401 taccaaatag ctttgcaaga agggcaaata tcctggaaat tagaaagtta tacaccttct
    56461 aatataaaca aagagtcata aacgcaataa ttaattagcg gggacgtaag atttctttat
    56521 ataagcatag agcactcact acataaattt aggtaggtat tgccaatggg agttatatat
    56581 aaagatatcg tttattttgc tgaggatgag catccctaaa tttctcgagg taaaaatgtt
    56641 tcatattgtt ttatttgaac ctgaaatacc acaaaataca ggaaatatag gccgtctttg
    56701 tgtaggtaca gaatctactc tacatttaat tcaaccaatg cgttttatct taacggataa
    56761 agcactaaaa agagcaggat tggattattg gccaaaccta aatctgcaaa ttcataacag
    56821 cattgaagaa atctatgcta tatttcctca ggaacgaatc ttcttagcaa gcaccaaagt
    56881 aaaaaaatgt tattgggata ttaaatacca aaaagacgat gtttttgtct ttggacctga
    56941 atccagaggt ttacctatag agcttttaaa taaatatccc gaacaaggaa tccgcatacc
    57001 tatgagtaac aaaattcgca gtataaatct tgctaattca gtagctatta ttatgtatga
    57061 agcagtaagg caaattaact gtttttttta aacaccttct tccagaggga tattcaattc
    57121 ttctgttccg ggaacaacaa atctgccatt tctaataata tcgtatcttt ccccatcttt
    57181 agttacagca gttatagatt ccagcatttc atagggcagg gttatatcaa tatgtttatg
    57241 agtatatgca tttagcggat cttctttacg agtaatagat ttttcgttat ctacagcaat
    57301 catctcttta gagtttacaa aactgggatg ggggctatct tcttcataac tgaaacaggt
    57361 gtcaccaatg gcaaaatgag gccccatttt ttctattatc aatatgggca gtaaaggtag
    57421 gatatcatat ttcttagcca tctgataagc caaagtgttt gttccaatag caaattctcc
    57481 aatcggaaga gtcttatgag gcagcaacaa attttcgtat atatacttct tgccttcttc
    57541 cggatcatca aaattcgtac aggaataacc tttaatccag ccatcttcaa attgaatttt
    57601 caaattataa aagcgcaaag aaccaagata aatatcatct atatgtagtg ttccattagt
    57661 gcctgttaat aggggagaag taaaaacctc accaacaggg atattaacat cggctacaca
    57721 attttcaaag ttggtttctt tatcaggatt ttgcagagga tgcaatttaa cattgatatc
    57781 ggtatcattt cccggtttgc ctttcacatt tacataatca gctttatcca acacatcaat
    57841 tatattctgc tgaattttgg catagtgcat actatccaga aggtttaatt ttaatgtatc
    57901 agcaaaaatt tctgggaagt gttctccaat ttcaggtgaa gggaaagcga taatgcaaaa
    57961 actgccttga tctcttctat aatattgata ataaatttga gcggatttag acataaattc
    58021 tctggaaagt tcctgttgct catcagttaa agctaaagca gtgttttttg ccacaggtga
    58081 aaaaggtgtc tctccaaaaa gttctacata aacaggtcct gcatcaccgc taaccaagtc
    58141 tttcaggtct tcaactactt cagcatagac atttattttc atatctgtat aatccctatc
    58201 tataataagc gcattatcaa aacggtgatc gtaagtaaac tgtttgtttt cataaatctt
    58261 ataagggaca ttaaattgtc cttttatccc tattttatcc agttcgttat aaatcaaaca
    58321 ccccaatctt tccattccgc aaggaacaat aatacttgct gtttgcttaa tccgataatt
    58381 tttgttggag cgggtaaaac catcaatcca gcactgaaca atataacagg caatatcatt
    58441 tagttctttt tccggatagg cattgataaa ttgagccatt ttgatgttaa cttcagaaat
    58501 aaacattcca taacgataga gataacgcaa atcttttaga ttagtattca ttattatatc
    58561 gtagtttatg ttcgcttcct gaatgtaact ctgataaaat gtccgataga gttttaattt
    58621 atggttttct gtcacgattt tctggtattc atttttccaa tccgtatagt ttaaatttcc
    58681 ttgcctcgct ttatcgtata aactaaaaaa caagcgcaat ttctcttcta attgcaagta
    58741 atttctattt gcttgtagat agatcatatt agcgaaatga aaatacagag cggacaaaaa
    58801 tttgcccata tcttcattat atagagcaac tgcataatcc gggttcccaa gacagttttc
    58861 atagtttgct aaagatgcat aatattcatt atgcaatgca gatagctgtt caaagggagt
    58921 tgtttctaac ttaggatcat tttcaaggat agaaccatac ttctcactta agaggcaaag
    58981 ctctttcact tctacaaaga attcttcata aggcatatta ggcattatat tggtagattt
    59041 aagtttttgg atcaatgtct gatatggggt tttacaggca aggattgtac tttggacatc
    59101 ttgaaaattt gctttcataa tggtttcctg ttatattata tttcgggata gaaaatatac
    59161 ccctgatgga aattcataaa ttttgaaagg gaagatttgt gtcaagcatt aatccacaac
    59221 ttccgctatt aaattttggt aaaaaatgca gaatagatac tctatatttt ttgaagcact
    59281 cgagagcggt gacacaattc aaatttgtca ccccctctaa tgttctggag cgatatatat
    59341 cttgacacga taatctatat taaaattaag ttctgaaatg aaatttatct gctaagtata
    59401 cagattatgt taggttctat aaaacctgag gagagaaaat gagaaaaccg cttttattga
    59461 ttatgctact actgattttt tcaactattt atgcagagcc agtttggaca aatgatgttg
    59521 ccataagaga agtgaattat attgactggc aaagaaacgc ggttaaaaat gccaataatg
    59581 aggtctttct cacttggtct caatccgaag atggcattaa taacctttat gtgcaaaaaa
    59641 cagatttttc tggaagtccg caatggcaag agccattagt cattaaagct gggtgtgtcc
    59701 aattcaacca agtaaaacaa cttgttactt ccgatggaaa tattatcata acttggctgg
    59761 aagaagataa ttcagtttct tctctatttg tacagaaata tactccagca ggtgatttat
    59821 tgtggggtcc gcaaggtatt aatctatctt ctctccgcgt catagatagt tttgaatata
    59881 gaatagcggt taataatata ggcggttttc tgcttttact gagaaatgac agcttatccc
    59941 aaattaattt gcagagttat gatgcctctt gcctacccgt ttgggataat agcgatctaa
    60001 atataaattt ggatcatcct gaacttgatg atttggttgc agatggttta ggtggagcaa
    60061 taatcaaatt ccgcagattc agtagtgtta atggaattaa ttatttacgc cgctttgcca
    60121 atgatggtgc attaatttgg gcgacaagct atactttaag caacggagaa ataagatacc
    60181 cacatcagtt atttttaaat cccaatggta ttttatatga tctgggtttt gcttatagaa
    60241 atgattccat catcaatatc agaacttata atgctttagc agagcagata aatcaacctc
    60301 tggcaactta tcaacttgtt cttgctggag agactaatat tgagaattgt aatttgaaag
    60361 cagttactac caatggtgga gatctgcaaa tcggattatt gcgtaatacg ataaataacg
    60421 attgcgagat ctttacttac aggttcaatc aaaatttgca gcctcgttat ccggtcaatg
    60481 gacttgtact ttccaacgcc ctttacgcta ttgacaattt tgatctggct gtaaacaatg
    60541 atgaagtggc ttttatctgg gctgaatata acgaagcaac cggtagcatt ctgaaaatgg
    60601 aaagaaccaa tattcaagga agccagtatg gctatatata tggttataat ttagaccagt
    60661 tgagcggtgt ttatgattca cctcagttat tatatcaaaa caaccaaatg ctagttatca
    60721 gcaaaaagac aatagatgaa tatctgaaat taagcaccga tttttatact ataacgacaa
    60781 aagaaggcaa agaccctcag cggactgatc ttgtttctct tattaacggt agtgctactc
    60841 ttttagcaaa tatcccatta gcagatcggg ctatgttgtt ctattctgat tccagagggg
    60901 gagaatatca tttatactgt cagggtatat ctttaactgg acaattgtta tttcctgaaa
    60961 acggaaaact gatcaatctt ggtcagttgg actataattc ctataaaatc tttaaaataa
    61021 attctgaaca aatagggttg cttcactatt ccaatgctct ctatctgcag attatatccg
    61081 aaaacggaga ttttttatta aatgggaatg gcattcttat ttcatcggct caattaactc
    61141 gttataatgc aacctgttat gaagatgata tatatatcag ttgggttgag ccaagttcta
    61201 tatattccaa tagttgcata tatggacaaa gaattcataa cggcactaaa atgtggggtg
    61261 aaacaggtat tatgttgaag gacttgatta gctcacccta tggttatgct tttacaggaa
    61321 gatatctatt atggaaagag acggtagctg gcaataatac tatcctatgt ttgaaatatg
    61381 atgaaaatgg ctatcctgat gcagactgga atgcttccgg agaaatagtt attaataatt
    61441 tgcccacaaa tcaatattct attgttgcca gttatataac aggtgaggat ctcttgttgc
    61501 tgattgcaga atatgttatt catccagttt tcttccaaaa agtaactgcc aatcatactt
    61561 tgccttgggg agatgaaggg ataacttttc ctgcaacatt ttttcagcta aattataaat
    61621 ataaagcaga taaactttat cttctctaca ctgctagaga tagtggcagt agttcatata
    61681 atgtcttctt ccaggtaatt gacagtggtg gcaattttct gtatccagaa atgggattgc
    61741 aagttaatac tttagcacct ggtaatgatg tctcttggag tgattcttat gctaattatc
    61801 ctactctttg cgtttttgat aataattctt gtttaactgt ttggggttcc agaatgtcga
    61861 gaacggatgg acaagatctt tattaccgca gaattaatgc agatggtaat tttgtggaaa
    61921 gtgaggatca attactttgc aatgcacctt atgatcagat atatccatta atatccacta
    61981 ttggtaatca ggcaataatc ggttggcagg atagaagaat gagtcattat actaattgct
    62041 atggaattta tgcgcaaaga atcgatgctt acggttcttc tctgccacct gagattactc
    62101 cggaagttgt tctattcaaa tcttgctatc ccaatccttt ttctaaaagc actaatatcg
    62161 tttgggaaca aaagaaccat tctcgagtta gtataaatat atacaacctc aaagggcagc
    62221 ttgttaaatc tctaacacca agttccatgg ctttagggga gcataatata gtttggaatt
    62281 ctgatgatga aaaaggaaag acagttggca acggtatcta ttttattcgt ctgcaatatg
    62341 aggataaagt tataacccgt aaagtaatgc acctgaagta attccctatc cgaattgata
    62401 gccatataat gaaataggcc tcaacatcag acgaaaatgg cattttgcct tggatttgtc
    62461 tgattgctat tgcgatcctt gattttaggt agtaacaacc taatcgttat tggattccgc
    62521 aaatatgcat aaatagcaac aagttagtgt gattttgttc ctcgccttgc tgttccttgg
    62581 aaattcgaac ccgagcttca gctaaaaaaa agaacgaaca gagtagaaat tatgtctttg
    62641 ctctttcaac ctcgttgcca tagcatacag aaacgtttta tagaaaacca taaaacaaga
    62701 aatctaattg ttcaactgct aaactatgaa tattgcagaa agaatgatag ataaagtgat
    62761 ttggtaatcc cttctgctta tttaaaaaac catattcttc cttaatgagt tactgtggag
    62821 ttacaatgga gcctcctggg tttttgccga gtagcaccac ggtatctcta ccacagttca
    62881 ttaagcaagc attaatttag tttctacaat aaagaagcaa tttcagctca attggtaata
    62941 atgccaattc ccagtctatt cggatagtcc ctaagttgcc aaattctcat tcagcaaatg
    63001 ctatttaagc atatatgttt cagatttttt ttagcattcc attttgcaga tgcacattca
    63061 attagcactt accttgatat ctatatttgt gtaaattcat attggctact tactataatt
    63121 tcataactga atggccaaat gatgaatttt ttggtcatga tgtttcgttt tgaatccaaa
    63181 ccgagcctca gctttaaaag gattgagaaa cggaaaaaaa tgtatgattg ctctcaaaac
    63241 ctggttgatg cagaactaaa atagttcaga taggaaatag aacatagtgg taattattat
    63301 ctatggtctc gtataataat agggtaaaag aacaaaataa agggaaaata gtattttccc
    63361 ttattgcaca agtggctata atcttataga tattagtgtg attagctgtt cattatgagg
    63421 tttttgatat tttggtataa gcaatatgca atgctttacc tccaaaaaat ccagctgcag
    63481 cttggacaat atttaccggt aaatctgcaa ctgcaacagc aagaccaaga ttggggaaaa
    63541 gccagcaacc gaaaaaataa cccgcaacca taatgacaac tgcgatggca ggatataaat
    63601 tccgttgcca tcttatgcct ggttttgcaa gtcctgctac taaaccttct aaacctttaa
    63661 taacaagagt tatgggagca aaaacaggaa aaccgataag atctgcaaga gcagaaccaa
    63721 ttccacctgc gattaatcct gctttccagc cgccaaaaag tccgctgaaa ataataacaa
    63781 catcccctaa attaaaatat cctccacccg ggacaggaat acgcacaagc agcgtagcaa
    63841 ccattaccat aacggtaagg ccgacaatga gataccattt cattttgact cctttatttt
    63901 atcttcaagt tcaatcttat tcaccggaat tgaattcgga actgtataac aatgccagat
    63961 tcgttcatca atcatttctc ctttcagagt tgcctcattg atcacataag tgatgagtgc
    64021 caaaagaaca ataagatgtt tttgaaattt gctttttaaa gtagcattct tttgctgctc
    64081 ccctggaatt gaatccacaa aaagacacag ctcggaataa gatgaaaaag caattctata
    64141 aagcagctta cctaagataa tatacttcag tttatctata ccgataggaa aatctaaaaa
    64201 agaatctatc cgcagccagt taattaaaac ctgcataaac aatagactgc taagcaaaag
    64261 agtagtcctg gtcaatccat aatgaatcca gtgagctctg cttttcccaa tttgaaaaac
    64321 ggcaaaaata agatatacgc caaaaaggag caaaataaaa ggtaggagat gatagatctt
    64381 ctttataagc cccttagcag aaaaagtcca ggcactaaac aatataacta ttaataacat
    64441 agccaattga atggttaaac tctccaagta aacaatgcaa aataacacga agaacaaaac
    64501 cagcgctaag gcaaccagga ttaatctata tagtggcttc ataaattata ctctgttttg
    64561 gatttataac tttaaaattg acgatgttaa catcttcaga gagagaaaat gaagaatgcg
    64621 cacagtaaat aagacaaaag ggaaagtcat tttcctttag atatcggtgg atttcttgta
    64681 tataacggtt ttcatctaca atgcaataaa ccggtctggg atcttgggaa aaggtttcaa
    64741 agagattatt caataaatat tgaacacagt ccaattcact tcttaagtcc aattcaggtt
    64801 tgtaaaaagc ggaatgggat ttaattgcat atccttgcaa atgaaaaagc tgctgtaaat
    64861 agatacggta aattagatct tttttactct ccaatacagg taggagacag tgtattaaaa
    64921 aagagctttt gccactgccg ttttcacctt gcaaatggta tatctttcct ggttcgaagg
    64981 tcaatttttt ttcaatcagc aagctaaaat cctgccaaca gaaaataggt ccctgaggaa
    65041 tctcaatcat tattttcttc cagaattata tctttaccac agctgttaat caggttttta
    65101 atggcttctc gacgtttggt atccagggat tgcattaaat tcttaaagtg aatttttggg
    65161 gcaggtgaat aaagaactaa aagcaccatc agaattactt tctgtcctcc tgaaagttca
    65221 ttcactttat agaatctgtt tatggctttc cccgttagtt cttcaaactg attaatcagt
    65281 tgattagcag tagaatcatc tatattgtat attagcaatt catctcgtaa ttcatcacaa
    65341 gaaataagtg gaacatctgc atctgctatt aaatagtcct tatgaatcat caaaactcca
    65401 aaatgcaact ggcggagagt cagggattcg aaccctgggt acctttaata ggcacaacgg
    65461 ttttcgagac cgtcccgttc aaccactccg gcaactctcc gtttattttt atattctttt
    65521 tttgcgaaaa aaatctgtaa gcaagctgga acattcgctt tccaaaatac cgcttacaac
    65581 tatagggtga tggttgaagt ttttatcctt caaaacatta tatattgaac caaccactcc
    65641 cgctttaggg tcctttgcac catagacaac agtgccaatt ttacttaaaa taatcatacc
    65701 ggcacacatc aaacagggtt ctaaagttac atataatgta taatcatata agaacccggg
    65761 ttctgaaacc agaattttgt caataaggag tttttcacaa tgcgctaaag gattggctaa
    65821 ctgtctactc cgattatgtt cacgcagaat aatgtttccg tttttaacaa gtactgcacc
    65881 aacaggaatt tcatcttcat tcgcagctac ttttgcttct gcgattgctt ctttcataaa
    65941 agtataatgg cttctatcgg aaaagcaaat catggtttgg tataaataac tactcttgct
    66001 tttttaactt gcttatccag ccaatcctca aaccagccat caggcaaatt ttgggcataa
    66061 attgttttga tgcgttccgg gtttggtttg gtttttcctg gtcggtaaga aattacttta
    66121 ggcactatga taatatcttt ttgagttatt tgaggaagag gagctttttc cagatatgct
    66181 ttaattgcct gataatatat attagtaatt ggtttgccct cccaaaggga atctatatta
    66241 atattatcca attttaccat ttcgtaggtt gcaggaaaac tgttatcggc aatccattgt
    66301 tgaacaagct ttctattttg ggctattcta tgttccttgc aaagttcttc tataatttct
    66361 tcacgcactt catccaaact tctataatcc tgagtttgtt tttccacaac ttcaaatata
    66421 taccatccag attgttctgc agaatataga ggtcccaaaa tatctcgtgc ttgggcatat
    66481 tttaaagatt ttattagctt acccttaaga tcatttgcag gaacaattaa cgaatcaggg
    66541 ctcatagcag gtgttacaat atattctata tcaaattcat tggctgcttg ttccagtcct
    66601 atatccaaag caagtttcac aatttgcttg gcttgtgttt cgggagctag taaagtaact
    66661 gatcttggta atgattgcac ataaattgta ttaaaaagcg ttagattttt tgtgctttgt
    66721 attttttgat agatcatata acccttttca taggggacag gctcactgca ttttccattt
    66781 tccaagccat ccagagcatt aaagacagtt aaaggtaatt cttctgtttt ttgataaccg
    66841 ttatcaataa aactaacaac cacatttttg ctatctacag aatttaatac atccttagct
    66901 tcttttccac tggaaagctc gttgcggata ttttctgcta cattttttgc ttcggaaagg
    66961 tctatgctat cgggaataac aggaatcgaa caataaccta atcggtaaaa ttgccataaa
    67021 ctatatttct ccaaattagc ttgataatag gcactaattt ctgcatcact aatagaaata
    67081 tccattttgg aaggatcaaa aatatataat tctaaatcag cattactggc tatgatttct
    67141 gaaacctgtt tacggatttt gggagttagc atttcctttt ctattaaaac actttgcagc
    67201 ttcatattgg gaattagata ttcctgataa tgctttcgga ggggcattaa attttctggt
    67261 ttatccgtca ttaaagactg catatatagt tttttgtcga acttaccatt aactctgaat
    67321 ctgggggagt taattatatg ctccggaatg gagttggaaa gcgtatccag cacttctgta
    67381 atgctcgcag aaatgttata ctttttataa taatccttca aaataatggc gcgggtaatg
    67441 ttctgccagg tttcattatg tattttttgc ttttcctctt tggtaggagt gcgtccagtt
    67501 tgatatccaa aaatggaata gtgaccgcga tatgaactat aaaaatcttc taaagaaatt
    67561 tcagtgccgt ttacgcttcc ggcaatatta gtactatttt gagcacaaga aatcaggaat
    67621 attgttccca aaaaagtgat aattgttaaa ataagaattc tgttcatttt tcccctaata
    67681 gtttattctg gattttttgt aagtactgcc aggcttcaac aggtgaaaga tcattcagat
    67741 caatttcctt tatatcgttt aaaacagggt cattttcact agctttatca actaaaattt
    67801 caaatatatc aatttgaggt acatccctaa ctaatttttt acgaatagta gctgttaagc
    67861 cctgaggact tatttcgtgt tcttccaggt ttttcaatat ttcttttgca cgcttaataa
    67921 ctctatcggg aattcccgct aaacgagcaa cttgaattcc atagctttga tctgcgcctc
    67981 ctctttcaat tttgcgcaga aaaatcattt cgtcattcca ttgctttact gccacattgt
    68041 agtttttaat atccggatag aggttttcca gttccgtaag ctcatgatag tgagtggcaa
    68101 aaagagttag tgaatgtttg tatttttgta tatgttcaat gattgcccaa gccaaagaaa
    68161 gcccgtcaaa agttgaagta cccctaccta tttcgtctaa taaaatcaga gattttgggg
    68221 tagcagaatt aagaatattg gcggtttcta tcatttctac caaaaaagta ctctgtccct
    68281 gagctaaatt atcggaagct ccaactctgg taaaaacacg gtcaaaaata ggaagtgtca
    68341 tttcggtcgc aggaacaaaa cttcccattt gggctaaaat aactaaaagg ccgacttgcc
    68401 ttaaatatgt agattttcct gccatattgg gtccagtaat tatggctatg ctggtttcag
    68461 gataatttag gtctgtatca ttggggataa atttatcact ttccatcagc ttttcaataa
    68521 ctggatgcct gccttcaact atgtgtaatt ctctactttc ggtaaaaact ggtcgcgcat
    68581 attgattctg ccaagctaaa tatgctaatg aagaaagaac atccagttca gcaataactt
    68641 cacctaactg ttgaaatcgg ggcaaagatt ctgctaaatt ttggcgcagc tctttaaaca
    68701 gttcatattc caagttcttt attttttctt cggaggaaag cacttttgcc tcaaattcct
    68761 taagacgcgg agaaataaaa cgttcactat tagttaaggt ctgtttggga atataataat
    68821 ctggaacttt attcttattg gcagaactta cttcaatata ataaccgaaa acacggttgt
    68881 aaccaacttt taaattattg ataccggttt tacgacgttc atcttcttcc aaacgagcta
    68941 tccaactttt gccatcataa atcagatcca taagttcatc taattcagga ttataacctt
    69001 tggcaaaaat acctccttcg gttattggaa taggtggatt atcgttaata gatttttcaa
    69061 ggagagcgat aatatcctca aaactaccca tattttgtgt ccaaacagta aattggggat
    69121 gcataaaaga gcttagtttg ttttgcaaaa tcttggctga atatagatat gacttcaagg
    69181 aaattagctc tcttggattt attcttaaag aacctaatcg ggtaaccaat cgggtgatat
    69241 ctccaatctc cttcaaaatt gaacgcaatt cttttaagtg actgcttttt tctatacagg
    69301 attgaattat atcttggcgg aaagtaattt cttctatgct taataaagga tgcaggagcc
    69361 aatgttgtaa aagccgcgac cccataggag taatagtttg gtctaataca gaaattaaac
    69421 tcccatattt tgtcccataa cggatagaac gaactaattc cagattccta cgactgattt
    69481 catccaattg catatattgc gaaagagaat agtaccgtaa agaagtgata tgattaagcg
    69541 gagaagaata taaagattga acataagcta aggcagcacc agctgctgta gctcccaatg
    69601 ttttattatg tgctccataa ggttctaaag tgtttactcc gaaatgtttt ttcaaagttg
    69661 taattgcttc ctggggttga aactgccagc tatcaaacac tgttatagtc ggtaatttat
    69721 ccattggtaa ttttttaatt agttcttctt ttcctgtgct atctactatc aattctgcag
    69781 cattaaatct ttgtaattca ctaactaatt cttcgctatc caattctgta aaaagaaaat
    69841 cgcctgtaga aatatctaaa tgtgctaatc ctattttacg cttaaaatca gtaaaatata
    69901 aggaagccaa aaaaacattg gcattacctt ccaagagaga ttgatctaaa accgcaccag
    69961 gagtaattat ttcagtaact tcgcgtttta ccagtccaat tgctttttta gggtcttcgg
    70021 tttgttcaca aataacaact tttaaacctg ctttaattag tttatctaaa taggtttcca
    70081 aagaatgata gggaaaaccg gctaagggaa caggattatc atcgttttta ttacgcgtag
    70141 taagagtgat attcaaaatt ttggaagcgg tggaagcatc ctcaaaaaaa gtttcgtaaa
    70201 aatcacccat ccgaaaaaga accagtttat ctggatgctt ttctttaaca gcaaaatact
    70261 gttttagcat cggagttagc ttgttttctt ctactttgtc cactgttcta atatgctaca
    70321 agaaaagtag ttatattgtt tgtagccaga aggttcttgg cctcttctgc tgttattcta
    70381 tctggaaaag aaccgcaaat aactacccag ctttttttcc ctccactaat atcttcataa
    70441 taagtggcag ggatatttag cagacggata tttactacta aacggttagc attgctttct
    70501 atggaaaatc ttcctgcctg tagccagaaa ccttctttgg gtttgctttt aagcttaatt
    70561 gaaagatcct gtttgcttac tggttgagtt gccactattg gagctgcagg ttttggttca
    70621 attttctgaa ctgtttttaa gctatctgct actgtttctt ccgcgatcgc aatctgcaat
    70681 ttaggatagg gatagagaaa actgagatct atattgcgat ttctactttt taactctaat
    70741 agacgatcct caatttgata ggcaatttga gaatatctat ttaattcgta acctcttcgg
    70801 taagcattaa cggcatcaga taatttatcg gaaagctcgt aagcataacc aagacggtaa
    70861 tagaaatact gttcatccat ctcaggaagc ttagcatttt gcaatttgat aagtgtagta
    70921 agggcactat atgctttctt ttgagcacgg tagcattctg caattaaata ataggcagat
    70981 tcacaaaatt ctccagtcgg gtctaaacgt agataatttt cacaattgtt tatagcggtt
    71041 gcgaaatcat cctgccacca agaacataaa ccaagccagt agaaacgttc aattaaatct
    71101 gtggaattta tccgtcttaa atacacagtg gcttcctcaa ttttcctttc cagaatatat
    71161 attttaccta attccagaag ggatttttga gcataaggac ttttgggaaa tctttcgatt
    71221 agccattgat gagcagatat ggtatcctcg gttttagttt ttagcattgc attataatat
    71281 cccaggcaag cacgttcttc atcattaaca ggtttcagat tacttatagc ttcagatagc
    71341 tcatccagct ttccattgct atacaaatta ttcagttcct gaaattcctt tctaacagcg
    71401 gagtaaacaa ctgtgcataa actaatcaag ataaaggaaa aaaggattcg cttaataact
    71461 gaatagcgag ttggaatcat taattgctca ttgaatttag aagttcttcg ttggtatcgg
    71521 ttgcctgcat cttcttgcgt aaggtttcaa tcccttgaat gggagagatc gttttcagat
    71581 atttacgcaa cacatacatt cggtttagtt cattttgaga tagaagtaat tcttcgcgtc
    71641 gggttccgct ttttactaaa tctattgcag gatacaatct catatcactg atggagcggt
    71701 ctaaaacaag ttccatattt ccagttccct taaattcttc aaaaattacc tggtccattt
    71761 tgctgcctgt atcaatcaaa gcagtagcaa ttatagttaa actacccgcc tcttcagtgt
    71821 tacgagcaga gccaaagaat tttttgggtt taattaagcc attggcatca ataccaccgc
    71881 ttaaaacttt tccgctggaa ggagttatat tattataagc tcttgccaat cgcgttatac
    71941 tatccaaaac gattactaca tcctgattga gctccaccat ccgtttggct ttttctaaaa
    72001 ccatttcgct tacggcagaa tgattttttg gtgattcatc aaaagtagaa ctgataactt
    72061 ctctgttacc aggttttagg atttttttca tttcagttac ttcttcgggg cgctcatcta
    72121 caagtaaaac aataagataa acttcaggat gattgctcaa aatagcattt gctgtatctt
    72181 gtaacaaagt tgtttttcca gttctgggag ctgcaacaat taacccacgc tgcccttttc
    72241 caattggagt gaataaatta attatgcgag tgctaaagtt atttggatca aattctaagt
    72301 ttaagcgttc agtaggataa tatggggtca attcatcaaa ggaccgtaaa ttcaacattg
    72361 aatttggttc cagataatta accgcttcca ccctaagcag ggcataatat ttttctcctt
    72421 ctttaggact gcgaacaggt ccactaatca tgttacctgt tttaagccca aatcgtctta
    72481 tctgagttaa tgatacataa acatcatcgc gaccagcatt ataattgttg tcaggaaagc
    72541 gaagaaatcc aaatccatct tccataattt ccagacaccc agtaacgaag gcaagacctt
    72601 cttgagctgc ctgaaattca agtagtttaa agattagttc ggttccttta tattgtgtat
    72661 agccaggcaa accgattttt tttgccaagc gggatagttg aagttgactc aagctaaata
    72721 gatcctcttc aaacagcatt agttatctcc tttatcagcc attaattata gcttattttt
    72781 tttttataac cggtaattgc taatcgacag gcaccggcat atccaatatc tttaatccgt
    72841 tccatcaatt cttttccttc tgcaattatt gcctctgtgt tttccattgt ctgcaagcgt
    72901 tttataatat cttgacaatt gcgtatcaat gcaggacaat ttttactggg atcatccaca
    72961 ggtacataga aatctgcgat ctccactttt ttcaggggta gtttttttac tatatcttgc
    73021 agttcttggc gggtaaaagt tttttgatgg tatgttccca aaaggcgatc aacagaagca
    73081 acccaatgat gcatcataat atgagtttcc tgagcaggtg tttggtctcc atcagaatac
    73141 atttctgtga tcagcatcaa gccctctggt tttaaaactc ttagcatttc gtttaaaacc
    73201 gcttctcggt tttttatatg gtgaagggca tcagaaatac aaacagtatc aaaataacca
    73261 tcttcgaatt gcatatcttc caggttcatt cggtatattt ctatattatt ctcagggaat
    73321 attttttgtg cgtaatccac acaacgttgc gaggtatcta ctccaattat ttgtgtaaaa
    73381 gattttaggt gttgtttcaa ggtattgata aaatctccct ttccggttgc ggcatctaaa
    73441 atattaccac catccacctt agacagtatt ttgatgattt cattcataaa agaactcctc
    73501 gcttgttatt tatttgggat tagtgttctt tggtataatc ttctcagatt gagcagttca
    73561 gataactgca aattatctca tattttcaat ttctattatt ttaaaaagcc agttttccgt
    73621 caagtattat ctttaacagg aatgttcaga ttgggtagat gctgaattag atttctataa
    73681 tgtttacgaa tagtaggcat aaatctgggc tctttatagt ttgctaaagt ttccattgct
    73741 tgatggcgtc caaaattata aaattctcgt gctttatgaa aagcgaaagg acgaccatta
    73801 ggcatacctg cttcaattac gatatctgga ctgaacattg ctatatctcg aattgccaga
    73861 tatgcttgat tttgcaaaac agcattgaaa attacttcag ttctgcttaa acgcttggtt
    73921 ttagtttctt cttcactttc cagatttatg aaactggcac ttctggatgc ttcaggtaaa
    73981 acattaacag cgattacttt atcttcagaa agcaaagagg aaaaagccat aggtaaggga
    74041 tgttcaattc ctccatcaac aaaaatgtat tgatttagtt tataaggagc aaataataca
    74101 ggtaacgaag aagaagctct cattgcatct gcataaagtc ctttattgaa aagaactgtg
    74161 ctcttacttg ttaaatcata agcacaagca ataaagggga tttcactttc ttctatgctt
    74221 ttaccctcag tccaggttag aaataaattt aataaactcc ggccatcaaa aatacctttg
    74281 atggttttat ctaacttgaa gggagaaaat aatttggcat tattaaaact gcctgcaagt
    74341 tcatatatct tagcggaact tagtccggaa gaatataatc caccaattat acttcccata
    74401 ctgcttccga taattccggt tatttcaaat tcctcttcta aaatacttaa aaccccgatg
    74461 tgagctaagc ccagtgcaga accaccccct aaaatgagag ttgctttttc tttcatagta
    74521 tattccctta tttttataac cacttcaaac aagattgctg aaaggtcaag ttttatttgt
    74581 atagtagtaa attgtgtagg taaataatcg ctagcatctt cgaactttgc ttgctaaaaa
    74641 gaattacagt ctaatagaat aaacataaat gatggtaaaa aatctcttac agatcataag
    74701 atatgaaatg aatagagtta gttatgtttc tgaattcata aaaacagcta agccagacta
    74761 tatccaatgg gaattatgct tatttatcca tcaatagcac attcctctgt tgtaacaata
    74821 aaaacctcga caaaaattca tatcttcaaa aataggtttt aaggaatttt gtagccaaga
    74881 taagatattt gtcaccctct atatatatgg agaaggaaat tgaatttaat tcttcttcgt
    74941 agaattctga tgctgtaata ggctataagt aaaataatta tcgtcgctat tagtcctttg
    75001 tgtctgtaag tttttaagaa aaagcatcgt taattaaagt acaaatgaat gctaacttaa
    75061 attgggagta tatttatgat caaagtagac aacctgagca aagtctttat aaaaaaagac
    75121 ggaacgaatt tttacgccgt aaataagata aattttgaag caggagatgg agaaatagtt
    75181 tgtcttttag gtgtaaatgg agctggaaaa acaaccacaa tgagggttct ttccactatt
    75241 tttcagccct cagaaggctc tgcagaaatt cagggatggg atattagaaa gcatccagat
    75301 aaggtgagag agaatattgg ttttctatct ggagatacag gactatataa tcgtttaagt
    75361 tgcagagagt tcatcactta ttttggacat ctttacagca ttgaagatca acttatagag
    75421 caacgaataa aagtgatggc tgatctactt gatatgaatg aatttctgga caaaaagata
    75481 gagtttctat cttcaggtat gaagcagaag gtttcaatag tgcgttccat tattcacgat
    75541 ccgcctgtaa tgatttttga tgaaccgact gccggtttag atattttaac tgccagaaat
    75601 attgtttcgt ttattcggga ttgtaagcaa cgagggaaat gtgttttatt ttccactcac
    75661 attatgcgag aggcagaacg tttagcagat agaattgtta tgattcataa agggaaaatc
    75721 ttagcggatg gcactttgga agaactgcgt aacatttctt ctcagataga tttggatgat
    75781 atcttcgtgt attacattaa tcaacataca gacgaacagg agctgagagc caatgaattt
    75841 taaacatact ttagtaatct tccgaaaaga gttaatggaa gttttgcggg ataaaaggac
    75901 tatttttact actttcattc ttcccattat attatatcca cttataattg ttggttttaa
    75961 ttcagttatg atgcgtcaga ctaaagtgtt agaagaaagg ggagctacca ttgctgtcca
    76021 ggatagtgta gataatgaaa tttcgcatcg cttgattcag ggtttagtcc gcatcaagaa
    76081 ttacactttt ctcccttata acgaaagcac aaaacagcat tatgcagaaa aagatattca
    76141 atgtatagtt actattcgag attctttagg cagcgaggga acccagtttt ttaaaatata
    76201 tataacatac gataaatcta aagatcaaag tagaatagta tatgaaaagc tttctaaaca
    76261 gctttccaaa gtagaaaaag agctgcaaca ggagcgttta cagatttctg gattaagccc
    76321 ggattttttg aatttagtag atattagaga acgagatact tccagcgctc agaagaaaat
    76381 ggggatgttt ttggggatgt ttttacccta tatagttatt atgatgttat ttgcaggtgc
    76441 ttcaattgtc gcagcggatt tggttgctgg cgaaaaagaa aggaaaacct tggagacttt
    76501 acttgtttct tctgttggaa ggacagagat tgttatgggt aaatatctca ctatcataac
    76561 tttggctatg cttaatatga ttgtaaatct tttcagcatc agtttttcct tacagtttat
    76621 gctggcaaat cagagtaaag aaatgtcagg tgtaactttg cccattaatg caatgttgat
    76681 tttacttatt gcaatgattc ctttagcaac tctatttgct gctttgcttg tttccatttc
    76741 tactttcagc cgtaatatca aagaagcaag aagttacgaa caaccattat taatgatagc
    76801 aatgctgatg gggatggtca gttttattcc tactatagaa atttccaatc tgctggctat
    76861 agttcctgtt gttaatatag ctttactatt caaagcggtt ttgattaatg aatatactct
    76921 gtcgcatatc ttaattacca taataagcac tttagtttta gatctaattg ccattctgat
    76981 cactgtaaag ctgtttaaaa cagaatcagt tcttttccgt actgaagaag aaagtggagg
    77041 tataaaagtt ctgaaaaaga aacccaaata tgccttaact ccttacaacg gcatagttta
    77101 ttttacaata gctttaattt tattatacta tttaggtgga tattggcaaa ctaaagacct
    77161 gtataatgga ttaatccaaa ctgagctcat tattattgct cttcccgttt ttatagtttt
    77221 gaagctatta aagctgaaac cgaaagaaat cttaagattt aaaactccca aaccagctgg
    77281 tcttatcctt gttcctttta tagctatatc tgcttcgata attgtatcta tcatgtcaca
    77341 gctaataaat acgatttttc cttatccgga aaaatacaca gaggcactta ctcagctttt
    77401 taatatgaat gaagctccct ggaaagtctt tttggcaata gctttgttgc ctggaatttg
    77461 tgaagaactt ttattccgag gatttttaat tcgttttttt gaaaaatacg gtttacaatg
    77521 ggcagtaatt atcagtgcaa ttctatttgc tgctttccat cttgatcctt ttcgttttgt
    77581 tccggtttta cttttgggtt ttttattagg atatttggct ttgcgttcgg gttcagttta
    77641 taccagtatg ttatcgcata taataaataa tggattagct tttatactgg ttacatatag
    77701 caattctagt tgggttaaat tcttagtatc ggagggggat aatatacact attggttatt
    77761 tataccggct ttagttgttt ttgttatctc tctttatgct tttcataaag ttactgccca
    77821 aggagaagaa gtatgtgtgg aatagttggt tatattggct atcgtaatgc tttacctatt
    77881 gttgtagaag caattaagcg gttagaatac agaggttatg atagttctgg ttgtgctctt
    77941 attcataaag atgaattgca agtatataaa aggcaaggga aaattatcga attggaacgc
    78001 tgtttgccag aaccaaataa atgtagtgga aatattgcta tagcacatac tcgttgggca
    78061 acccatggag aaccaaatga agttaatgct catcctcatt tggattgtaa aggggaaatt
    78121 gccattgtcc ataatggcat aatagagaac tataaaatat taaaagatag attattggaa
    78181 ctggggcata cttttataag cgaaacagat acagaagtta ttgttcatct aatagaacag
    78241 tatttagtta gcgaaccaac tcttgaagat gctgtttgtg aggcaatgaa aagagtagaa
    78301 ggcacttatg gattggttgt catatctata aaagaaccgg ataaattaat tgcagtgcgt
    78361 aaaggtagtc cgctcattat tgggattggt gaaaatgaac attttattac cagcgatgtg
    78421 aatgctatca taattcatac aaaacgagtt atctatttac aagatggtga attatgtatt
    78481 gtaaaaaaag ataattttga gattactact ttggataaac gcagcgtaaa accagaaatt
    78541 tcagtagtgg attgggatat ttctgctata gaaaaaggcg aatataaaca ctttatgttg
    78601 aaagagattt tcgaacaacc catcactata gaaaatgctt ttcgaggtag gataaatcaa
    78661 caacttggaa ctgcacgctt agggggattg catattccta gctttgaatt gaaaaaagtt
    78721 aaacaagttc atttagttgc ctgtggaact tctttttatg caggattaat cggtaaatac
    78781 ataattgaag atcttgcccg tattcctgtt tttgcagaat atgcttctga atatcgttat
    78841 cggaatccaa ttattccaga agatacttta gtatttgtaa ttagccaatc aggggaaact
    78901 gcagatactt tagcagcatt aagagaagct aaagctaaag gagccagagt tttaggcata
    78961 acaaatgttg ttggtagtac cattgccaga gaaagcgatg ggggcactta tatccatgcc
    79021 ggaagtgaaa ttggagtagc cagtaccaag gcatttactt ctcaagttac tattatgacc
    79081 ttgctagcaa tccttttagg aagattgaat catctatctc caatccaggg gatggaatat
    79141 attaaggagt tggaaaatat cccagccaag gtagaaaaaa tccttgctca aaacgaatat
    79201 atcagaaata ttgcagctac tattaaggat tgtactaatg cactttattt gggtagagga
    79261 ataaattatc ccgttgcttt ggaaggagct ttaaaattaa aagaaatctc ctatatccat
    79321 tctgaaggat atccagctgc tgaaatgaaa catggtccta tagctttaat agatgaaaat
    79381 atgcctgtga tcgcaattgc tacaaaagat ccgctctatg ataaaatata ctctaattta
    79441 caagaagttc gagcccgcaa aggacgcttg ataactatcg ctaccgaagg agatacagag
    79501 cttgctaaaa tatcggaaaa tgttatctat atacccgata ctttaactaa tctacaacca
    79561 cttttaaccg ttattcctct gcaattgctt gcctacaatg tagcagacct gaaaaatttg
    79621 gatgtggatc aaccgagaaa tctggcaaaa agtgttacgg tagaataaaa tctgaagata
    79681 tattgccgga cagaaatatc ctatatccct ggtatgatat tatctctact tttcagtatt
    79741 ttcgatgcta gatacagaat caatttattt aggacgcctc aagtccacct tttttaggac
    79801 acttcaacaa cccatactcc tctcctcctc ctctcctctt caacccatag ttaaggatga
    79861 gtgaagattg ggtggatgag gagttacttc tggttaactt gcaagagaaa gtaaggttta
    79921 gcttaagata atctttatat gttaagaatg ttgcacaata aaacatcatt agcttggagt
    79981 taaattataa aatccaggtc actttttctt aacaagaatt tacaatacgc tgtaccaatt
    80041 gccataaatg ctatatttta cttcataaat ctcaacttga aaattgtgaa atcagttaat
    80101 gatgattata gttttggaag taaggataca ttaaaacaag catccgcaca attgaaatta
    80161 aaaaaggagc ggtggttata gtatttcttt attaatggca ttcacaacac aaaaaacccg
    80221 gttctctaaa aaaccgggtt tatagtttgt aaaagttagt tttcttattc tttttcttca
    80281 ggttcttctt cggttgcagt ttcttcagtt tcttccattt tgggcataat agtaatatcc
    80341 acagaggact tggcaatttc gggatagaaa tcatcgatat attcacggat agtattatcg
    80401 atgtcggtat gtttatctaa aatttcctgg ctgaagaaag ctctgattct taccgaattg
    80461 agagtgttgt taacattaac ttctggcatt tcgccataga cattattcaa aaattcaggc
    80521 agggatttgg caagtttggc agtgccgcta atttttttgt atgccttgaa accaaaatga
    80581 gcagcagcta cggttccacc taataaaagg agctttttaa aaatcttttt cattattgac
    80641 acctcttctt cttattttat ctgtaaatgg atttgattct tccccaagaa cggttctgga
    80701 tgaaagtcgg ctgcagtata ttatagtccg caggggtaat aggaccaagg gcaaactggc
    80761 tgaatccata aacaacaatt cctgttaaag aagcatattg agcattggtt gacattttgc
    80821 tgtttcgctg agaattaaat aaattgcaaa gaacaaaaca ggttccgctg ccatcggtaa
    80881 ccattaagct gtttttggag cttttatttg ccatagtaga tacatttaaa aaacgaatat
    80941 atacactctc gtatgcttct gcctcctcgg gatgagctaa ttgaccagta gttagaagaa
    81001 cgggctgagg aagaggatta ttagtactga gcactctgtt ttgagagata ttctggagac
    81061 aggtcatccc gtaatattca gaaacagtgc cagtagtttt aacataatca ccccgctgaa
    81121 ctttagcatt tctatccaga attaatatcc ctctccaggg accatttagt ttttcactaa
    81181 taaaaaaacc gccttctcga taatcagtag cagtaacaat tccttctaaa gtaacatctt
    81241 tacccatata tggagatgga aaagttccat cgcttcctct tgaagaagtg aactgaactt
    81301 catatataga aatggcttct acctgacaaa cagcgcaagc cagaatactg attaggatta
    81361 atattctctt catctatacc tctataatta cataataaag cattccacta tcctgtcaag
    81421 taaaatcgta ggcttaacaa tggggaaaag gtaaaaaagt taaatcaggt tttcatatcg
    81481 ttatatcttt catttcacaa gggacttacg cttacctcgt ccataattgt tcaagggtcc
    81541 ttctgtgata acaggttcaa tttggcaaat tcaacagaga ataaaagtaa ctcaaattct
    81601 gctaacgatt cttattaact atcatcatcg aaataattag tataagacta gtaatttaaa
    81661 attacgagta aattcaccga attttactga gccaatataa atacccttag ataaaggtct
    81721 attattatta tcaacaagcg agagaggaac ttctaattta cctgtatcta taattggcag
    81781 tatttctgtt cttagctttt gtcccttgag gttgtataga caaaatctcc ccgaacattt
    81841 tatagttgct tccagatata aggttatttt atcttttgct ggattagggt aaaaagacat
    81901 atgaacttga ggtatatttt ctgaactatc aaaaatagca aggggagttc tgcgtaaaat
    81961 aattccatca tacgcgggta tagatacaga agtgcatatt tgaccattgt ttacaatatg
    82021 atcctgacta ccattaagat gataccaagg atgggttaat tctgcagtaa gagttatgtt
    82081 atacggatta catattacca atccattttc aaattctctc ataagtaatt cttcctccat
    82141 taagcgtaaa cctatgtcat caaaatatac aattccgggt tcattaccaa gattaaagga
    82201 aaagcgaagt tgatcaggat ttaagcttgt gttttgagag gtaacagaga aaatataatc
    82261 agtccaatca gtatttaatt caattggaat actatgagga ataacccatt cccagttggc
    82321 aagatgtttt gagataacaa aattgattgt tctgggctga gcagattttg ctttgaaagt
    82381 aaccagatat ctattaccgt attgaaaatc catattggga ttattatatt gcataaattg
    82441 agcgctgtaa tcatttccat ccgcataggt gacattacat attgcaaatg aattattatt
    82501 ttcaaatcca ttttgtaaat atcctgtagt gggtgataca aatgccaatt cccaacccgc
    82561 taaattagaa ttcatttcac cattaatcaa caaattcggc tggtctgaaa tttggggatt
    82621 tacaaaacct tctaatggct cgcccaaatc actatcatat tcatcaaacc accatgtatc
    82681 agaatgatca gaagggccaa gatcaattgc ataataagca gaacccatca atgccgttgt
    82741 aagagtaaag cgaaaatgag aaatatcagc aagaaaccca tttggttcca tagcttgaat
    82801 gatattcatt actggcggga cgaatgtatc ttctatgtta ttatacatat tcataaatgt
    82861 tgaccagtcg taaccaaatt caaaattagg attattatag tcccagccct caaacataac
    82921 tccattaaga tattgtccat attcataacc tccatttccg attagaatct tagtgggatt
    82981 ttgaattcta aattgattta acattgcttc cattcccaat atccactgac tatcaagcca
    83041 atccctgtta tcaactatcc cgtcattatt acaatcaata ttggcaatct cgtaggggac
    83101 aatagaccag caattgtcta taaaaaaacc atcccaaagg ggattattaa gaacattgtt
    83161 attaataaaa tctgcaaaat gttctatcca ggtaaatcca tttacttcag gacaaaaagg
    83221 agtacaattt aacatccagg ttccttgcca aaaagaagta tgttctcctg ttgccgtcct
    83281 taaccaccat tcatcttgaa ttgagtttct taaggaatag cgaaggggct gataaattgg
    83341 attgggaata tttgtactaa tttcttggca ggtcatataa gccagcagtt ttacattggg
    83401 gtttagttgc cttatactta gtaaagcggt aggattgatt tcactcattt ccatatccag
    83461 aattactaaa tcatattttg aaagtgcgga tactagttga ctgttctggt cactcataaa
    83521 acaatcaaga taataacagg ataatttggg ataattaccc ataatataaa ctgggcataa
    83581 tagaagtact aaaagcgaaa tgttcataaa aaatttccac atatttccct ctcttttttt
    83641 tacctgtgtt cataaaataa ttataatcga ttatggagaa aaagcaaata gagtaaattg
    83701 atggggattc gattataatt aatactcact gttaataaac gaagtaagga agcgaaaaaa
    83761 ttatttcgtt actgatcact cataacatat aatcctctta ctggatatat gtctctatta
    83821 tacattttca ttctgaaaca gtcgtcattt ttttttcatt tgacagccag ccaaatgaga
    83881 aaagacttgc cctaaaatga ggtgaaattt gaaaacagct attattatta aaggtgctca
    83941 ggaacataat ctaaaaaata tagatttaac aattccacgc aatacccttg tggtctttac
    84001 aggtgtatcc ggttcgggaa aatcatcttt ggctttcgat acactatatg cagaaggcca
    84061 acgtcgttat gtagaatctc tttctgctta tgcaaggcag tttttagggc agatggaaaa
    84121 acctaaggtt gattacattg aaggtctttc tcctgcaatt tctatagaac aaaaaaccac
    84181 aagtaaaaat ccacgctcta cagttggaac tgttactgaa atttatgact atttaagagt
    84241 cctatttgct cgaattggaa aacagtattg ttataattgt ggtaggcctg ttgggtcgca
    84301 gactattgat cagatgattg aacatctgat gcaaaataaa acgggaacaa aattgcaaat
    84361 ccttgctccc cttgtacaaa atcgaaaagg tgaacataaa gatgaattag aaatgcttcg
    84421 tcaggaaggt tatgtccgcg tgattatcaa tggacagctg cgcaatttgg aagaagatat
    84481 tattctggat aaaaaaagca agcacaatat agatgtagta gtggatcgca tagttattaa
    84541 agaaggcatt gaaagtcgtc tttccgattc tttggaatta gctttaaaac tggcagatgg
    84601 gataattaaa attgattatc cagaagaaaa acaagaagtt attctttcag cacaaaatgc
    84661 ttgtccccat tgtcatattg gttttgaaga actatcacct cagagttttt ctttcaatag
    84721 tcccattggt gcttgtactc tttgtggagg gctgggttat aaacttgaat ttgatccaga
    84781 tttggtaatt agtgatcctg gattgagtat tatggaaggt gctgttgctc cctggggacg
    84841 tctagaggct aaacaacata gttggacaat gaatacagta aagaatcttg caagagcttt
    84901 tgatttttcc ctttctactt cctggaagaa tctcccggaa atagttaaac agcttattct
    84961 atatggttcc aaaggtaaaa agttcaaaaa ttcctggcag aatgaaaatg gcagaggtga
    85021 attcttaatg cgttttgagg gtgtaattcc tcagctggaa agaagaatga acgaaacaac
    85081 atctgaagaa atgcgccgat attatcttca atatatcggt gataaacctt gccctgcttg
    85141 caatggaaac aaattgaaac cggcatccct tgcagtaaaa attgctggta aaaatatcag
    85201 tgacataaca gcaatgagca tcagtgaagc atatgacttt tttgctggtc ttcatttgaa
    85261 aggaaatgaa ctgctgattg ctgaagaagt tttgaaagaa ataaagaacc gtttgagctt
    85321 tttaaagaat gtcggtttgc attatttaac tttggataga aggtctccca ctctttctgg
    85381 aggcgaagct caacgcattc gtttagctag tcaaattggc agtcaattag ttggtgtgat
    85441 gtatattttg gatgaaccca gcataggttt acaccaaaga gatatttcta aacttgtctc
    85501 tatgcttttg cagcttagag atttaggtaa tactgtaata gttgttgaac acgatgaata
    85561 tacaatacgc acagcagatc aaattgtaga ttttggaccc aaagccggaa tttttggagg
    85621 ggaaattgtg gcttcagggg atttggaaac aattatgcac catccaaaat ccttaactgg
    85681 agcatatctt tcgggaagta ttaaaatccc gactcccgaa aaaagaataa aacctgatgc
    85741 aagaaagctt agcattatca atgcgaggca caataacctg aaaaatctta atgtagacat
    85801 tcctctggga ctttttgtgt gtgtaacagg tgtatctgga agtggaaaaa gcagcttagt
    85861 aaatcagact ttatctccct atctacataa tcatttttat catacatcgc ataaagttgg
    85921 taagatggat gaaatacgcg ggattgaata tatagataaa ataattacaa tagatcaaca
    85981 acctataggc agaacccctc gttccaatcc ttctacctat gttaaattat tcgatccgat
    86041 tcgccaactt tttgctcagt tacctgcttc taaaatacga ggatataaac ctggcagatt
    86101 ttcctttaat gtaaaaggtg gacggtgtga ggcctgtgaa ggtgcaggtg taaaacagat
    86161 tgagatgcac tttatggctg atatttttgt aacctgtgag gtctgtaaag gaaaaaggta
    86221 taatcaggag acactttcta ttcgctataa ggggaaaaat attgctgaag tgctcgatat
    86281 ggatgtgcag gaagcaatcg agttctttga tgccattcct gctataaaaa gtaaactgaa
    86341 gaccttacaa caggttggtc tcgattacct taaattgggt caaccctctc ccactctgtc
    86401 tggaggcgaa gcacaacgca taaaactatc tcgggaactt agtaaagtgt ctacaggtaa
    86461 tactctttat gtgttagatg aaccaacaac gggacttcac tttgacgata ttaataaact
    86521 attgaaggtt ctgaaaaaat tggtggcaat ggggaataca gttatcgtga ttgagcataa
    86581 tatggatgtg ataaaatctt ctgactggat aattgattta ggacctgaag gtggtgatga
    86641 aggtgggaag ataatatgtt gtggaactcc tgaagacctt attaattgtc ccaattctgc
    86701 aaccggtata catttacaat atcacatgca aaaagaaaag gaactttctt caaattcctt
    86761 aactggatta gaaacagaaa tgagacctcg caaagtatca actaaaagaa aaacagataa
    86821 aagccaggca ttatgagtta tcagcgtttc agttatgctc cgcccacggc aggaaaacat
    86881 tcagttggta ttgcaggtga ttttagttcc tgggaaatta tctctatgga agaaaagggg
    86941 ggagtacatt atcttgcctt agaacttcca ccaggagttt atcaatataa atttgtagta
    87001 gatggttcct ggattctgga tgaaaataat cccaaaaagg ttcctgatgg ttttggagga
    87061 tataattctc tgttggttgt tgaacctgtt acggaagtaa taacttggga agatataatg
    87121 gctgaactcg cgaataaaac tccagaaaag ttttatcaat tctttcgttc aaaggaaaat
    87181 agctatgagt tgcgtttctt ctggtatcca gaattgtcag atgcaattta tctgctatat
    87241 aataatcagc aaattcagct tgcacacatc ggccaaactc ctttatatga ggtattttat
    87301 tgtttattcg aacattgtgg catttttact tttcgcctaa aaatccaata tcagaatatg
    87361 tttctgtttt tcggagctga gggcttttct gaagctgaag aagatataac tcctattgaa
    87421 atagaactaa aagctatctc cattttttcc attccagatt gggttggcca cagtataatt
    87481 tatcagatat ttcccgatcg cttttataat ggaaataagg ataacgatcc cgatttcaga
    87541 gaatggtatt atgaggattg tagaactcca ccttcctcca gtaaaattct ttctcccgaa
    87601 aaggaatatt atcatatggt agatgattgg tatgatatca atggcttgaa acaaagtcct
    87661 tggcaaaaaa agggggtgcc ggatttttat tctttttatg ggggtgatat cgcaggtgtt
    87721 aaacaaaagc ttgattatct acttgattta ggtataaatg ttatttattt caatccgctg
    87781 tggcaagcga aatctaatca taagtatgac agcgcagatt atcatagtat cgatccccat
    87841 tttgctacta ctcaggaaat gatagatttt gtaaagctag ctcacgaaaa aggaataaga
    87901 atcattctgg atgtagcttt taatcatacg ggagagacat tttgggcttt tcgtgattgc
    87961 gttgaaaaag gtccaaaatc cccttactgg aattggtatg attggaaaaa atggcctcta
    88021 cccaaacctc ttcctccgga ttttaatcct aaagattatt atcaatgttg gtggggtatt
    88081 aaagatatgc cggatttgaa ttatgacctt gctctaccgc atcctgaaga aaatgctgtt
    88141 cgcaatataa aggaagcaca tcctaatgca gctttggttg attatctgat gtctactgtt
    88201 cgctggtggt taattgatat tgggattgat ggtttccggt tagatgttcc agatgaagtt
    88261 cctttctggt tttgggaact gtttcgcaag gaagtaaaaa aagctaaacc cgatgcttgg
    88321 atagttggtg aaatctggta tgaagcaaga cagtgggttt cgcctctcta tttcgattcc
    88381 gtgatgaatt atgcattctt taaaaatccg gtaatcgagt tctttattct tagcttgcta
    88441 aataaggaag aattctgccg aaaaatagaa tccggactcg ctctatatcc tttccattcc
    88501 ttaaaggcaa tgatgaatct tttagggagc catgatacac ccaggatttt tgagataagt
    88561 aaaggagatg ttaaacgcct gaaactagca attgcttttc agatgtgttt tattggagct
    88621 cctcatattt attatggaga tgaaattgct atggaaggag gcaaagatcc tgataataga
    88681 agacctttta actggaattg ggaaaataat ccggtagctg gtgacttaag aaacttctat
    88741 aaagaactaa tttctatccg taaagctcat cctactcttt gtgaaggaca ttttgctttt
    88801 atcccagctc cagataaaat aattgctttt cagcgggaat tgaaaggtga aaaaattctg
    88861 gttttcttga ataccgaagc agaatcaata gctattgata tcccagaatc ctcaatatta
    88921 cttttaggga agatagcaga gaggaattat atattacctc attctgcatt gattttatca
    88981 gagttgaaat agagttgctt atagatagtg aaaatatatt gatgctgaaa taaaattcga
    89041 gaaaaagtga tgcctaagct gttcagcaaa agagaacatg ggatgtatcg atagattgtt
    89101 ggatagaaaa gtaattcaaa tatcttgact tcaaaccagc aatggtaaaa tgataatata
    89161 gaataaggag tatgaatgtg cggaatagca ggtatatata attttagtaa agagacagtt
    89221 attttaactg aagtaactgg aaaaaaaggt atattaaata cagaaccagc aggtttaagt
    89281 aacgacatag ataatttagt aaaaatagag ggtggttata gacaaaacgg agctaaggca
    89341 attctattaa agaaaatgac tgaagcaatc aagcatcgtg gtcctgatga tgagggctat
    89401 ctaataaagg ataaaaatgg aatcagatct tattcaggaa tggattcaac ggaggaaata
    89461 aaatcgttat atcctttatt taattcagaa ataactactt tagataacct ttctaaaaaa
    89521 gctgtgggaa agacagttgg agcagagaac ataatcttgg gaatgggctt ccgccgattg
    89581 agtatattgg aattgaaacc gattggtcat caaccgatgt atgataagga actgggcata
    89641 gcaatttgtt ataatggaga aatttataac tatcttgaac tgcgtgaaga gctaaagaag
    89701 aagggctata aattttgtag tcagagcgat acagaagtaa taataaaagc atatcacttt
    89761 tggggtgaaa attgcgtgca gcattttaac ggaatgtggt cattttctct atgggatgag
    89821 aaaaagaact tgctattatg tagtcgtgat cgctatggga taaaaccatt ttattatgcg
    89881 gttcagaatg gagttttata ttgggggagt gaaattaagc aacttatgct cactccgata
    89941 gacaaaactc taaaccaagc aatgatatgg cgtagtatga agataaattc tatgctggtt
    90001 tatgatgatg agacctattg gcagaatata cactgtttaa aaccgggaca taatttgata
    90061 gtaactaaca gtaaggttat tgtaaaacaa tattatgatt tggatataac aagtttcgaa
    90121 acttctcagc tatcttttaa tgaagcagtt gagcaatatc gaaatatctt tctagattcc
    90181 attgctctac aacttcgcag tgatgtagaa attggagcaa ctctttcggg aggaatggac
    90241 tcttctgcaa tcgtgtgttc tgcagtgcat caaaagaaag agccgcttaa aacattttca
    90301 tcttactatg ctttccctcc agaattggat gaacgcaagt ggatagagaa aattattaaa
    90361 gaaacttcct gtgcttcata tttgattagc cctaaagcta atgatgctat agatttatgg
    90421 gagaaattaa cattcttaaa tgatctacct ttagccgcag gttttgtatc gcaaaatgca
    90481 gttttgcaag aagctcataa acaagggatt aaagttatac tctctggtca gggatcagat
    90541 gagctatctg ctgggtacag acattctcta tatcgctatt ttgctgatct gataagaggt
    90601 gtgcatatta gtagactagg aaaagaactt ccgggatatt tgaaaaaaga caatcgggca
    90661 ttttccaagc tgagcaaaat agtgctttct acttttttac cagaatcctc actatataat
    90721 ttggagtttt gctattaccg gtttgaacct ttcacgcgtt cttttacaaa tgaagctgaa
    90781 atttcctgtg gtgagcagat attaaaaaag atagtagata taaaagcttc gcggctttcc
    90841 aatttcttat ataatatggt gcatactact tctctgcaaa ctctgttgca ttttgaagac
    90901 aggatggcaa tggcgaattc tattgaaagc agagttcctt ttctggacta tcgtttagtt
    90961 gattttgttt tttcgcttcc ttctcaatat aaagttaaac ccccttatac aaaagtaatt
    91021 catcgggctg caatgaaaga actgattccg gaagagattt atcagcgcac agataaaggg
    91081 attttttctt caccatttta tcagagttgg atgaaacaag aattaaaacc ctatatatca
    91141 gatatttttg ccagtagtac ctttaggcag agaggattat ggaatttgcc caagattaat
    91201 catttttggc ataaatattt agggggagat aacagtcagg tggaaatgtt atataatgtt
    91261 gttgccctag aaatatggtt tcaacagtat gaaaatcaat ctacaggagg attaaaatga
    91321 aaaacatagt taaaataata gctttatgct ttgttctaat ttctcttagt tcctgtaaac
    91381 ttttcaaaag tccaacagtt actaaaattc aagatgtgaa agttgtttca ataacccctg
    91441 ataaaagcac aataaaaata tcccttatgg tgaataatcc caattgctat aaactgaaac
    91501 tggataaact ggaagtagag ttactgaata aagaccgtga caaagttggt tatgctaatt
    91561 tagagaaagt tgtggaaata cccaaaaagg cagctatgtg tataaatttt aatgttgagc
    91621 tggaaacgcg tccggtagtg agaatgatta gttcgcttaa ccagaaagtt caatttttca
    91681 ttgtaggtaa aggacacggc aaagttaagg gtcttagcaa aaaattctat tttgatgagc
    91741 cttacgagct ggatttgaaa gaagaattaa aaaatattat tcccaaattc agtgtaggta
    91801 cccagaatct gtttaaaatc acgaaaacca gcgtggagaa gatgagcatc ggtcaaaccg
    91861 aattacatat ccattttatt attttgaatc cttatggcct tacttataat ttaaaaggtt
    91921 ttcctgctga gatttatatt gatgataaat tagctggaac gggaaacctg aaaaaccagc
    91981 tgaaattcaa tgaaaatatc ttctcacaag atgggataat ggtatttcaa ctaaataact
    92041 tgaaatccat ttttggtgct gtgaaaggtg cgattaaagg tgaaatcaaa tactctgtca
    92101 aagggaatgt aattattgat gcttttggta tgcaaataaa gcagccctac tcataccagg
    92161 attccttacc tgtaaatatt gtggaattga tactgaatta aaactgatgc ttgtttcgct
    92221 ttgactatca aaatggtagc gattaaaaga aagttctagt gtagtgtata aaatcctgga
    92281 agcacgattt tgcttgacat tttcctaaaa gcgcttatta taaattgtaa tgatacttaa
    92341 gccaaggaga taaagatgaa aaaatatttg tttttggtat tgattatatt acctgttttc
    92401 ttactagggc aagttagtag tgaggaaaca aatattcctg gagattctat gccagaagaa
    92461 gaatcggaat atccggaagg aactcaatgg ggtatgggtg gcgcagttgg tacagtaaca
    92521 attgataatc gaacctattc ccagattcgt ttacaaccag aacttcagat tggtaaattc
    92581 ggaattggtc tggatattga tctgattatt gatgctgaag gtaatgttcg taaagaggat
    92641 tgggatgaat tgcaggatta tgtaaataaa tttttatata ttagatttgc gcaacggaat
    92701 gatcccttct attttaaaat tggtagcatt ccaagttata cgctgggtaa tggtttaatt
    92761 ttcaacaatt attccaatat gatactctat ccaagtgtga aaaatgtagg gggttatata
    92821 ggtgttaata ccaatatatc aggtcttggt tttgaagcat actcccataa cattcgcaag
    92881 aatgaaattt tagcaggaag agtgcatatt caacctttgg cagcaaccaa cattccttta
    92941 ctagaaaaat tgcggttagg agttaatgct ggaatagacc gtgatcaaaa tgctaaatat
    93001 gaagataaag atggtgatca tattcctgat atttacgata aatttcccaa agataaaatg
    93061 cgatatttag atactgataa tgatggcatt gcggatgaag atgatataga catagacgga
    93121 gatggtattt tagaccatcc tgattataac ccttatatag cacaaaactt tcctggaatt
    93181 gaagatatcg ctcagggtta taatttggat attgttatat tccccgatac agcagccgcg
    93241 tataataaaa aggatgaaat tctaatctac agtgcagatt atcagttacc tttaattgaa
    93301 accgaccatt ttaacctggt taattatggt gaactagcca aaatcgacgg ttacgggact
    93361 ggaatgattt ttcccgggtt tagcagcaaa ttcttcattt ttgacgcaaa agtggaattc
    93421 cgtaattttg gtgatcaatt tattccagga tattttgata aacaatatga tcagcagcga
    93481 gctgaagtga aacatatagt tttggaggaa agcggaagcc agtattggtt cttaactacc
    93541 aaagaatcca atttggagaa tgttgaagct tctcttggct ggtttggcta tttacgcgct
    93601 aatctctttg atttcatcta tcccaaaatc gcctatcaag atatgtatgg gaagaataac
    93661 tttctgggaa aatccctatg gggtagtatt actataaatc catatatgat tcccaaattg
    93721 aaagaagcaa ctatatacta ttcacaaacc aatactaact atataaattt ccgatatcta
    93781 cgtaatgaaa acgcttacat ttcaggacgc ttagtttatt cattatccga taatgcaaat
    93841 ctaattggaa aatacagcga attctataca gatatcaatt gtgacggtaa aattaaaggt
    93901 aatgatgaag taattgaatc ctttactttt ggagtggagt ttcaatttta gacttcaata
    93961 agttgaagct ataaaaagat cagcagaacc ggaaatttga agcatttttt cttcagaggg
    94021 gatgaacatt gcttttcctc tttgtaatga taatccgctt tcagtgttca atgaaccccg
    94081 gaagcaaaaa acaagcatag gtttatattg gtttgttaaa atgtaattac cttccaagat
    94141 aagacggtag agatgaaatt cttctgcagg attaggatag ggaacaagtt cattcaattt
    94201 aagatgggga tgtaaaagtt tgggaagata ggtttcaaag cgagttatag caaaaaggat
    94261 atctctatct atatgctttg gggttagccc tccccgaata acattatccg aatttgccat
    94321 aatttcaatg ccagcaccct ggatgtatga atgcaaaatc ccaggttcca aaaatatagc
    94381 ctgaccaggt tgcagggtga aggtatttag taaaagaggg ctaagtacac cgctatcttg
    94441 tggatagaat tcatttaaat gtttacaaat ctggcaggtg aacctgaatt ttgggctaat
    94501 attattctta caattttcca tagcagagag agttgtttcc aattgagagg gctcgcttgc
    94561 taatatatgg ttgaataact cctgtagatt cttagagcaa ggtttcttct ggaaagaaga
    94621 taatgcctgc cagagattat ctatccccag gatttggaaa ttgcgaatga tttcttcata
    94681 aggtctaaat ccacacatag caataaaagg tgtaagggca cagattagtt ctggttttgg
    94741 attagggtct ttaaaaatac gattaggatc agttattggt attcttgcct cttcttcttt
    94801 ttggaagcca tattgtgcag tttgcctatc cgggtgtact tgtaatgaaa gtggttgggc
    94861 agccgcaagg attttaaata aaaaaggtag attattaccc tgatttttac ccaacatact
    94921 ttccggatat gtttccaata acttatccat agggatttca ccttcttcag ttataataag
    94981 agatggtgat tgaggatgtg ctccgaacca tgcttcagct aagggttttc ctgtcatatc
    95041 cagagcacca attagctctt gaatatattt tatatcaccc caagaatagt tttttaacga
    95101 gggtttaatt agataaggca taaatcatct cctcaaaatt gaaaataaat gaaaggttgg
    95161 attgctgccg aacggaattg aaagagaacc cagataagaa atgctaacaa caatgattgt
    95221 agaactaatg ggcattttcg gaagaatttt tccagattat tgttgtaagc ttctggcatc
    95281 cagtgaaata tatacccgag caaaattaag gcagcacata tatggtattg actgatccat
    95341 tgaaagaata tatttccgtg aaaatcagta aaaatgcgtt tcaatattaa ccaagcgacg
    95401 gaaaaatcgg gagaacggaa aaatatccaa ccaaaacaaa caaaatggaa agttactatc
    95461 actcccacaa atttcatcac tttggtttgc ataattttta actttagaat cattttatcc
    95521 agcactaaag cgataccatg aattccaccc caaaaaacaa aattccaaga tgctccatgc
    95581 cacaaaccac ctaaaagcat tgttatcatt agatttaaat actgtctggg tttgcctttt
    95641 ctgtttcccc ccaaaggaat ataaagataa tctcttagcc atgtggaaag agatatatgc
    95701 caacggcgcc aaaaatcggt taccgaagtt gcccgatagg gagtaataaa gtttaccggc
    95761 aaagaaaaac caagaagagc tgccaaacca atggcaatat ctgaataacc gctaaaatca
    95821 caatatattt gtaaggcata ggcataaact cctaaaagat tttccacacc ggaaaatagt
    95881 gctggattct caaagatgcg gtcaacaaag ttgatagata aataatctgc gataacacct
    95941 tttttcagta atccggaaaa gattaataca agagctttgg caattgtatc cttatctaaa
    96001 cttaacttct tatgaatttg gggaataaaa taacttgccc ggacaattgg tcctgcaacc
    96061 agctgtggaa agaaagatac aaaaaaagtg aaatccacaa aactcttgag cggttcaagc
    96121 ttaccaaaat aaatatccag agtgtaactc caggtttgga aagtaaaaaa tgatatacct
    96181 acaggtaaaa cgatatcata taaaggtagc tgaccatcaa aaatctggtt aacagtagat
    96241 atgacgaaat tagtatattt gaaataaccc agcgaaccta aattccatac aatgctaaga
    96301 ataagataaa atcgttttcc tgccttgctt ttagctctat aaataagtgc acccaagcca
    96361 aaattgatta gggaggttat gataagtaaa ataatgaaac taccgctggt tttgtaataa
    96421 aaatacaagg aagccaacat caaataccag gtacggactt ttaccttatt cacaaccaat
    96481 ggatagaaaa gaaggactac aataaagaaa aatagaaaaa aggtgctatg gaaaagcaac
    96541 ggctgttgtg ggtcgtaaac taaactatta aggatgtttt gaattaaggg aggcataatt
    96601 tattcttaac ctgctttttc gctaaaactc ttataacttt ttatgaatgc ctgatagaag
    96661 agataacctt gcagcatata acccttggga gtatagtgaa gcatatcatt agctgccatt
    96721 tgttgatttc tccatttttg aacactattg ttgccaccca tcactatgct taaattccac
    96781 catgcatagt gattttcttt ggcataatcg ataatcactt tttccacttt atctaaatcg
    96841 ctattatgct tgccccgttt atacgaatca ggtggtaaag taaaaagaac cggtgtttca
    96901 ggattatatt gtttcaattt atccataaaa gaaataagat ttttgctgaa aacttcgttt
    96961 cgataggtgc cctgagcatc gtttgtcccc aaagaaataa taattaaatc cggattcaat
    97021 agccctattt gttcaaataa ggttgattga gctgttaaat tataaaaact tgctccatta
    97081 attccacaag agtgataaag taatcccggt tgattatttt ccagagagat tccaaataaa
    97141 gtggaaggtt ctcccgcaag tgttagttta attttggtaa ctgctttatc ccaggaaact
    97201 ctgctgtaaa aataatttcc agctttaata gaatactctt tgatgttctt ttccgctaaa
    97261 tcaatttttg gtttcaaacc gggcatcgca gtaatcatac taactgtgtt aaagctatta
    97321 tcgcctttga aaaaattgtt tgtagtaatt tccagacaca ttccgttgtt agcattcaaa
    97381 gcgtaaccac ttaaaccacg ttcggaattt gaacggctga aagccccgct ggccgttatt
    97441 ctataatcat ccggttgatt agtccctgct aagcgtaaag ggaaaataag ccctctacct
    97501 gaattgccga aatatttttg taatgaagtt cttgctgttc cagaaaaata cccagattgg
    97561 atatgggaat cacctatttg ataaatagtt actctatctc ttaatccact acgcaactcc
    97621 agcaattttt cataaaaact ttgcaacgct tttttatcac ccgatatata atttttctct
    97681 ggattaatga acttgaactg tttcaagtat tcgttgcttt ccagaagcag atagtagctt
    97741 tgaataaatt cttccaaagt atccatatca gaaggatatt cctcttcagt aaaaagttct
    97801 gttgtgttat caactgttat attatcttct gcgcggctat tgttttgtag catcaaaaaa
    97861 gctaaggcca gaattaaaac agagaggaaa aaacgctttg acaattagtg tcctctcaaa
    97921 tagattagaa gcatttcagc aatatgatct gctcctgccc gggtaaaatg agtataatcc
    97981 tttccagcat atggaggttt atgatttaca tattttggca tagaattaat cccacccatc
    98041 gctgcaaaaa gattccagaa tccacaacct gtctctttag caacatttga ttgagttgcc
    98101 actaaatagg gaatatcagg agaagttata tactcagaac cctgctttat acttctatca
    98161 tgagcactga tgagtaaaat tggaactccc ggaagcgctt tttgaatatg agctatgctc
    98221 tttttcatcc ctcgttcgta ataactgtag tcccgaattt gataattgga tacatttact
    98281 ccataatgta aaattacaag atcatatttt aggtgctttt gaaatccgga aaggatatca
    98341 gagcgaatac gctgaaagaa cattcctgaa taacctcgaa tcggataatt atctatataa
    98401 gcacctgtag gttcatcaaa actaactcca taaatgtgca gagggtcata agcactgaat
    98461 tccaaacgaa cttttttaca agaaactgct ggactaaggt ctaacatctg cactccggac
    98521 tgattattta aatagcgaac tacaaaagga gaaccatcat atgaaacttt cacaaaacta
    98581 ctatcagccg catgactgta gaataaacga atacgcttaa aatttggtgc tccaccagca
    98641 gtggttgtgg cagtatattc tacccaggga ttataatcta cataaaaacg ctcactttct
    98701 ttatgaggtt ttggactaac tacttgtgag gtatccaaaa cagctaaact atcaagttgt
    98761 aagggttcta taactttctc tgcaatatag taattacgcg gaatataagt gtaaccggtt
    98821 attccaagag gaatattatt tctaccggta ctcataaaag aaatactttc ccaattacga
    98881 gaaaatttat gttttatagt ttgcctaaaa ccagatacaa ttgaggtaat accaatcatc
    98941 ccaactcctg aaccagaatg gactgattgt aattcttgtc ttagtttccc tgttatcaaa
    99001 tccccttcaa taatagaatc gccataatag gcaattctta tgcttttagc tgagttattc
    99061 atcttctgta taaatggctg taagggtaat aaattgttgg gaattgcagt agcttcttta
    99121 actactattg tatctgtagg ttctatcgta tctttcggtt ctatagattc cagccaagac
    99181 agtttcttaa taggaaatat tttattatcc gaatctggaa taacccttgc tattaactcc
    99241 actaatatta cagctataac tactataatc aggggtcgat aaaaaacttt attcatatct
    99301 accttttaaa acatctttgt attattaact ggttggttga tttcctcgtc aactctctga
    99361 gctacagtac ttaagttact ggataatttt tcggtaaccg tgtaactcta ttgtgtgtgg
    99421 aacaataaga tatcattctt ccacattatt aaagccaatt tttacacatc attcaatcgt
    99481 caagaactat ttggcttttt gtttttttac tttattatat atgtatacat aaggatatat
    99541 tcctcttttg gagaatgact ttttgtcatt ttcttatgaa tccaatgtct tttcagtaac
    99601 atcggtacaa aaaggtaatt tgaggaggat actatgattt ttagaagcaa ccgtcagaaa
    99661 gatgatcaat tgacaggttg gcttaagata tggtgtatgt taatctttct atttttatct
    99721 gggttcttat tagctcaaac cagaactgtt aaagtaggta tttctcaaaa tgaacccaag
    99781 atgtttattg aaccttctgg taagccagcg ggtatcttta ttgatatttt agaagcaatt
    99841 gcagctaaag aggattggaa acttgaatat tatgagggta gctttgatgc aatctattat
    99901 cgtgtggcga aaggggattt agatctaatg ccagatgtgg caatttctcc aacccgagaa
    99961 aaagatttgg attttcataa agaaccggtt ctatcttctt ggtctcaaat ttatactcgt
   100021 aaaggtttag aaatcaattc catacttgat cttgacggtc attctatagc ggtgctggaa
   100081 aaatccattc aacaaaatgt aattgaatca tggattaagg gttttggtct gaaagttaaa
   100141 atcataaaag ttcataattt tgatgcagct tttgctgctg ttgccaacgg tattgcagat
   100201 tgtgctgttt ccaattatct atacggcaga aggcattgta aacagtatgg tctggtagat
   100261 acaggtgttg tttttgaacc agcaaccttg tattttgcaa caaccaaaag taaaaaccag
   100321 gaccttctgg atgccataga taaacactta cttactatga agaaagtgcc taattctgca
   100381 tattatgctg ctttaaaaag atgggtttca aaagatgaag gttttatgtt acctgcctgg
   100441 ttgaaattca ctatattgat agttatcctg gctttagtgt taacagttat ctatggtctt
   100501 actttaaaac atcaagtaaa tattcgcact ttggaattgc gaaaaagtaa tcaggaaatg
   100561 gaacaaagaa ttattgaacg tactgcagag ctggaaaaag caatgctaaa agccaaagag
   100621 gctgatatgt taaaatctgc gtttctagct acaatgtctc atgaattacg cactccctta
   100681 aattctatta tcggttttac aggtatttta ctgcaagaat tacctggcca tataaatgaa
   100741 gagcaaagaa aacagcttac tatagtgcaa aacagtgctc atcatctttt ggatttgatt
   100801 aatgatattt tagacatttc caaaattgaa tctggccagc tggatttgca ttatgaatca
   100861 tttttcttga acgactcaat agataaggta gttaaaatag ttatgccgca agctgaaaag
   100921 aagggcctgg aattgaaggt ggaaatatcg caatcggaat tagaagtata ttgtgataaa
   100981 cgcagattag aacaagtgat attgaacctt atcagcaatg cgattaaatt taccgaaaca
   101041 ggatatgtgg ccattatagt acatagcgaa gaagacaaat atcagattca ggttcaagat
   101101 actggaatcg gaatagcccc agaagatttg gaacagttat ttctaccttt ccatcaaatt
   101161 gatacaggtt taactcgtca atatgaagga acaggtttgg gcttgaatat ttgtttaaaa
   101221 ctaataaata tgatgggagg tacaattaca gtggatagta tcccaggtaa aggtagcata
   101281 tttactgtaa ttatgcctta taaaaaggag tttacaggat gaaagagctg gttttaataa
   101341 ttgaagataa tccagaaaat atgtatttaa tgcgctattt actggaaaat catagttatg
   101401 aagtagaagg ggtaataact ggtcctgagg gaatcgaaaa ggcagaaaga attgtgcctg
   101461 atctaatttt actagatatt caacttccag gaatggatgg ctatgaagtg gcaagacaat
   101521 tgagacaaaa ccctgtaact aaaacaatac cgataatagc tgtaacttct tatgctatga
   101581 taggagatag agaacaagcc cttgaggcag gcgctaatgc ttatatggaa aagcctataa
   101641 atcctgatac atttgtggca gatattgaga agattatgga aaatattatc aaatagggag
   101701 gtaagaatga aaatactaat tgttgatgac catatgaata acaattatat gttggaatcg
   101761 ctttttaagg gttatggata cgaggttata accgctctaa atggtaaaga agctcttatt
   101821 aaactgcaaa cagagaaagt ggatttgatc ttcagcgatg ttttgatgcc tgttatggat
   101881 ggattcactc tttgtcggga aatcagaaaa gacgaaaagc taaaacatat tccctttatt
   101941 ttctatactg ccacctatac cggaactaaa gatgaagaat ttgcggagaa aataggtgct
   102001 aacgcttttt taataaaacc tgctgacccc agagatatta ttgatacttt agaaagggtt
   102061 atagaacaga gcaaagatga atcgttcgaa atttccgcta ctgcagaata tgaacaggaa
   102121 gtgcagaaat tatatagtga acgcttggta cgcaaattag aacagaaaat gttagaaact
   102181 gaacaggaag ctcttgctcg taaacaggca atgaagctgt tgcaacgaaa tgaaagattg
   102241 ttaagagcaa cccaaagagt tagtaattta ggaggatggg aatgggaggt cgcctgtaag
   102301 aaaatgtatt ggacagatca agtttacctt atttttgatc ttgatttgaa taaatataaa
   102361 ggaattgaag atcgaaaaat gtatgaactt ataatgcttt gttacaacga gactgataga
   102421 aaaaagatca aaagttcctt tgtgaattgt catattaacg ctatccctta ttccatagag
   102481 ggatggatta ctactgccaa gggtaataaa aaatatatat gtactgtagg cagtccgatt
   102541 acgaaaaaag gaaaagtaac caatgtatat ggttattttc aagatttgac aaatcgcaaa
   102601 tatgcagaaa tgaaagaaca tgaattgaat gaacagcttt cgcaagccca aaaattgatt
   102661 actatcggac aacttgcagg gggcgtagct cacgatttta ataatatctt aacggttatt
   102721 atggggaatg ctgaaatagc actttctcag atttctcccg ataatcctat aaaagcggat
   102781 attgaagaaa ttatgaaagc agggaaaaga gcttctaatt taacaaaaca gcttttatct
   102841 tttagcagaa aacaaatagt aaaaccagaa cccatattac taaaccacat tttagaagat
   102901 atgactaaga tgattagacg acttattaat gagaatatcg aaattgaact gaattgtgag
   102961 gaagaccttg cctttataaa atctgatgta gtacaaatgg aacaggttat attgaactta
   103021 gccatcaatg ctggcgaggc aatgccgaaa ggaggaaaat taacaattag tttgaaaaac
   103081 tacattcccg attctgcttt ttataaagag cacccagata ttcgtccaag taaatatgtt
   103141 ctattaacta tttccgatac tggaatcgga atggatgaag ccacccagaa gagccttttt
   103201 gagccttttt ttacaacaaa aagtaatcaa aatggcactg gactcggctt ggtgaccgtt
   103261 tatagaatta ttcaaagtac tagtggatat ataaaagtaa aatctgctcc cggtaaagga
   103321 acaactttcc ttatcctcat tcctgcaaca gaagaaattc ctgaagtgat tcccattgaa
   103381 gcacctaaac ctaaagttgc aggtcatctg gaaacagttt tagttgtaga agatgatgag
   103441 gctattcgct ttttaaccag taaagcagtt actaaattag ggtataaagt ccttacttct
   103501 gtaagtggag atgatgcctt gataaaaatc cgacaccaag agttaacacc ggatttaata
   103561 atatccaatg ttataatgcc cggtttaaat ggtatcgctt ttgcaagcga aataaagaag
   103621 tacttacctg gggtacaaat aattctcatg tccggatact cagataattt tctttcccaa
   103681 tacggaacca ttactcctga tatttacttt ttggcaaaac ctttcacgcg ttctgaactg
   103741 gaagaagcta tcagaaaaca actatcctta gatacctaac aaaaaaaagg atatcttccg
   103801 aaagacataa ggttaataaa ggatataagc taaaagagag atataagtag tatatactga
   103861 taaccaatag ctatgaaata tcaataaaga ggtgtaaaat gaagcaaaat acattgctcg
   103921 tcgtcctatt gatttttgct ggtgccttaa tgggcaatcc tcccgaatgg gcttggatgc
   103981 aaagaacttt ttgcaccgga aactgtatta tttataacct taaaagtgat tctcaggata
   104041 gaattaatgt aaccggctat tttgaaggtg aactcagttt gggagcaata actcttaatt
   104101 ctgcagggtc aagagatgcc tttgtggctc aacttgatgt tgaaggtaat tggctttggg
   104161 cttgttcaat aggtggaaca ggagctgaat atgcccataa aattgcagtg gatgcagaag
   104221 ataatatcta tgtaggaggt cttttttacc aggattttac agttggaaca acactattgg
   104281 aaaataatgg taatagtgat atcttttgtg tgatattatc ctctgcagga aatatactga
   104341 atgcaatagc tgttggctcc gaggatcaag acaccttaaa aggtttggct gtaactttag
   104401 atggaatttt gtttttaagc ggaaattata gatattccat tactattggc agcacaaatt
   104461 taactaatgc ttatgtggga ttatttatta gcaaatggga taaccagtta aatcctatat
   104521 gggctcaaca agcaagtagt gtatatggtt gtccagaatg tgcaaatttg ggaacggata
   104581 attttggaaa ctgttacata accggttatt ttctctatca atgtgcattt ggttatccta
   104641 atcaggtaat tttaaccagc caggaaacaa gtggttatat cgtaaaactg gatgttaacg
   104701 gcaatgcaat ttggggtgaa aggatcagtg atggcggaat ggggattatg gatagctata
   104761 ttgacccttt tggcaatagt tatattactt gtgatatttt ttttattata acagatagta
   104821 tgcaaaataa tcggttagat gcttttactt attgcggaaa attggatttt aatcaaaact
   104881 gggaatggta taatgatgat tctgcaatgg gacattgcat accaaccaaa atctgtggag
   104941 atgaaacagg taattgctat attaccggta agttatgggg caattatact tttggtgatg
   105001 atactttaac cggatatcat aataatgcca atatctatgt agctatggca aatccgcaag
   105061 ggcaatggca atggggattg caaacttatg atgctggcta tttattctat ctgaatatta
   105121 tagatattac tgctttagaa acagggggct gtgtgattac tggctccaat tcaactgata
   105181 gcttgcattt tggcgatttt tttattccgc cttcttccaa tagatattct tttatagtaa
   105241 aagtaaatgg ggaaacccaa gcaatttctg atcccggaat tgaatcaagt ccgcagattg
   105301 tattgatata ccccaatcct tcccacgata ttgttcggtt tcaattaaaa aacagtgagg
   105361 ggttaaccag tgaagcatca atttataata tcaaagggca attggtgaaa agctttccca
   105421 acagacaaaa tagtggagta tatgtcttga attggaatgg ttgtaatgag actggcaata
   105481 aagttgcttc gggcatttat atcttgagca tcaaaactga aaaaggtatt ataaagaaga
   105541 tctttacaag attataggat gaataaaagc tatattatcc attaaagaat acgattacag
   105601 cgacattggg gattcaagca ataaaggtaa aaaaacctta tctattgcaa agaaacgaag
   105661 ctcatataca gagcatcttt tatttatact atatattgat taaatattgg ggtctggctg
   105721 caaggaaaaa tgtaaaacag cttatatgaa ttgcttttag ctattcttgt ttttaaataa
   105781 tagtgttttt tatatgcttt cagtttacag ctctacggta tgtaatctca cctatcaaga
   105841 aaaatttctt gacttattaa actaattgac cggaatggtt cagttattca gttgtgattt
   105901 tataagttga tgataaacaa tataatataa ggaaggagaa tgagcgcgtt accgaataag
   105961 aaaaagttga tattggaaat ggctactcag ctatttaccc gtttcggctt tgccaagact
   106021 tctctggatg agattgctgc tgcagcacaa attgccaaag ggacagtgta ttattacttt
   106081 cccagtaaag aggatctctt tattgcctca atagaagtaa aaacaagaga gtactttact
   106141 atgttgcgtc agcatcttaa tgctacagag ggattcgagg aaaagttgac ggaattttta
   106201 cgatttccca tcaagcttat ttatgaaaat atgcccatcc tgattgagtg tttaataaac
   106261 attccccaga gttttcagaa acagttacat caacatagag aaagaagtag ggaagttatg
   106321 atgcagatac tttctgaaat aatccaactt gggaaaaaag aagatctaat tgatgatcgt
   106381 ttctctgaag ataggctctg tgaagtgata acagattggt ttgtaattgg cgaaccaggt
   106441 ttaaaaatag ttgatgttcc ccgcttgctg gaaatgattg agcgggataa tgattttatt
   106501 attaagatgc tactatatgg aattgtgaaa cgaggataaa atatgaaaaa gacactaatt
   106561 gtattagctc tgcttacttt tatattaagc agtgtgtatg ctatcgactt ggaaaacagc
   106621 atcacaaaag ctaaacagaa caataaggaa ctgttgatgg ctcaggaaga aatcagaaaa
   106681 tctgatgaat cttataaaca ggttcgtggc tctgcatatc cacaattaaa tttaaaaggt
   106741 gcttacggat tgagtaagac ctattatcca gattcagcaa taccttccaa aatggatata
   106801 agtgcaggtt tagatgattc acccacaaat aatgaagaat atttggctgg agtgataaac
   106861 agtgtggtga attctatgat tccaacctct cctgcagagg caggttcttt ttctatgcaa
   106921 ctgcaaatga accagattgt ctattctggg ggtaaacttg gaagcgggat aaaagctggt
   106981 aattactatc gtcagcttca gagaatcaat tataagatta tagaacagaa tgttgttcta
   107041 aaaactacag aactgtttta tcagtgtttg ttagcacaaa aactatggga agttcaggaa
   107101 gatggtttac aaatagccag aaggcatttg gaacgcgttg aactttttaa tcaggaaggt
   107161 caggtttcag aatttgacct cctacaagct cgcctaggtg tagctaaatt ggagccccaa
   107221 gttttgaaag ccaaaaatga ttatgatata gctgtttctg cttttcggaa acaaattggt
   107281 gaagaagata gtgatatagt tcctgaaggt gaatttgtcc ttcctgaaag aatagaaatt
   107341 actttagaag atgcaacaga gcaagcttta aaacaaagaa atgaaattga actgataggc
   107401 ataagcacac agatgaagga attgcaatat aaggtagaga aaattaatta tttgcctaat
   107461 gtagccctca gtgctgatta ttcattgtat acatcagcgg atgaatatgc tatacaaaat
   107521 gatgatttcg ggcataaata tggaatcagc ttaggttttc agatgcctat ttttactggt
   107581 ttgactaata cagctaagcg agattatgcg cgctacgact atcaaaaagc aaaactgcaa
   107641 caaagagata cggaagaact gatcgcttta caaataaagc aggattatca aaagtattat
   107701 cacgcttggg aaaatcataa tgtacaaact cagaatattc atctagctga acgcggattg
   107761 cagcttgccc aagtgcgtta tgataaccat gttggtatcc agcttgaggt ttttgatgcc
   107821 caattaacac tacaaactat taaactgcaa tattatcaat cgatctatga gattatatct
   107881 gctgatcgaa acttaaaaaa atcaattggt atagccctat aaatggagat aataaaatta
   107941 tgaaaaggta tttagtaatt ctaacagtcc tgatcctgat actcaccttt gcctgttcta
   108001 aaaaaaagga agagggaaag agtatggaac aaatatatag cgaggaagga attcccattc
   108061 gccaaacagt tgttgaacca tctaccttcc gtcaaaacct tctttacaat gcaactctta
   108121 atggaatgga agaaaccact gtccaggcaa tggtttcaga tgtaattacc agaattaacg
   108181 ccaaagtggg cgatagagtt gttaaagatc agattatagt ctgttttcca cagaatacac
   108241 catctgcaca gtatgaacag gctttaacag cttttaatag catcaagaca acttatgagc
   108301 gtatgcaacg actttttgcc caaggtgcaa ttagtcagca agatctggat aatgtggaaa
   108361 ctcagtataa agtatctaaa gctaatttag aagttagcga caagatgata aatgtccgtg
   108421 ctccaattag tggaattatc actgctatga atgtgaatcc gagtgaaaga gcttatccag
   108481 gaaaagatct atttacagtt gcttctacaa atggatacaa agctgttgtg atggttccgg
   108541 ataccgaaat taccaaattt agaaaaggtg ctaaagcaac cgctacttgg ttagatacaa
   108601 ctatttctgg aagagtatca gaaatatctt tagcaatgga tagttccact aaagcattta
   108661 gagtggaagt ggcctttccg gggatgaata aaaaaatcaa ctatggagtt acagcagaga
   108721 tatctataga aattcttacc aagccaaatg tgatagttgt agagcgtcac caaatacaaa
   108781 gtaataatgg gacaaaatat gtatggttga atcaagatgg taaagctgta aaaagagaaa
   108841 ttactacagg attagataat actcttgcct acgaagttat cagtggtttg aatcctggcg
   108901 atattctcat tactgaaggt ttaaatttgt taacagaagg cgcgaaactg cgcgttattg
   108961 aataaggatg ttggaactat gtttttaaca gatctttcaa ttaatcgacc tgttttggtc
   109021 actatggcaa taatggtgtt tatagtgttt ggagttcttg cctattttaa tttgcctcta
   109081 aacctgatgc cagatttaaa attgccttat gtagttgttc aaaccgtata ttcaggagct
   109141 ggtccacgag aaattgaatc tcaagttacc gagccgatag aagaagcagt tgctacagtt
   109201 agtcagattg actttatgca atcatactcg atggagaatg tgtctattgt gatggttgct
   109261 tttaaattag gtaagaacat agatgttgcc aatcaggaag tgaaagacaa ggtggatgcc
   109321 gttattcgca gtttacctga tggcacagat agacctatag ttctgaaaat ggatattact
   109381 gcttttccta taatggactt ggttctttca ggcaatatgc ctcccaagga tttatatgaa
   109441 cttgctgatg gagagctgaa agatcgtata tctcaaattc caggtgtagc gagggtttca
   109501 ttaactggag gagcgaaaag gcaaattgat gtacggctta ctgataaggt tgtttttgag
   109561 aataatatat cattatcaca attaagtgga attcttgctg cagcaaatct ggatatgcct
   109621 gcaggaaact ttacgcatgg aactcaggaa tattcagtaa ggttaaaagg tgaatttcag
   109681 aatgtgaaag agatagagaa tactgatata cccacagctt ttggagtaaa aaaactatcc
   109741 caattggcac gagtttcgga tactactgaa gaagttcgtt ccaaagcaat ttatttcaat
   109801 attcaggaga acttgaaaga cgacaatatt gtgcgtttat ctattactaa tgcttccgac
   109861 ggaaatgttg ttagtgttgc agaagaaatt acaaaacaaa taccaatttt aaataagggt
   109921 cttcctgagg gtgtgaaact tgaagttatg cgtgatgata gcgtctttac caaagacact
   109981 attaattcta ctttagtgaa tattcttctg ggtataattt ttacaggatt ggttctcttc
   110041 atctttttac acgatttgcg ttcaaccctt attgtagcaa tatcaatgcc ttattgcata
   110101 atttccacat ttgtatttat gcagcttttt ggctattctt tcaatattat gacaatgatg
   110161 ggattatcca cttcagtagg aatattagtt tccagttcag ttgttgtttt agagaatatt
   110221 ttccgacata aagatatggg taaaaccaga aaacaagcag cccaaatagg aacaaacgaa
   110281 attggtactg cagttttggc atctacttta accaatattg tggtattcct ccccattgct
   110341 actatgactt ctatggtagg acgattcttt acggaatttg ccataactgt aactgtagct
   110401 acattatttt ctctgttaac agcttttact attactccga tgcttgcatc caaaattata
   110461 cctaaagaga aaaaagttag taaatgggga caatcatttg ataaaatgtt tgataaattt
   110521 agtaatgtat attccaaatt tttaagctat gttttaagat caaagaaaac cagcgttagt
   110581 atccttttgt ttactcttgt ggcgttattg atttcagttg gccttgcttt tgtagtgggg
   110641 atggaaatga taccaaatgt agaccaacgc aatcttactg tttcaataga aatgcctcag
   110701 ggaagcaatt tagaagaaac agctaaaaca atggatataa ttcaaaatcg gttagcaaaa
   110761 cataaagaaa tcgtgcatat actcacaaat cttggttcca gtggctttat tgatacagga
   110821 accaatttag ccagtgctga tatcaaactg gtggatagac aggatcgtaa atattctgca
   110881 aatcaattag tagatatctt aactaaagag cttgctgata taccaaatgc taaaataaaa
   110941 atagcaggtt cttctatggg catgggaggt ggaggccgtt ctgctgtgga atttatgctt
   111001 gaaggtcaag atattgatcg tttggaacag cttaaaaatg aagttattaa agcgattgaa
   111061 gatataccag gtttaatcaa tttagacact tccagccgtt ggggcaggtc tgagcttact
   111121 ctctatccca aaagagataa acttgctgca gcaggagcta cagtctatga tttagcatta
   111181 gctttacgag caaatgtaga aggtttagtt tctaccgaat acagagaaag cggtaatcaa
   111241 tacgatttaa aaatttctct ggaagatgat gtagttaaca ccccggataa gattagaaat
   111301 ctgaatgttg ttgtgatggg caggaattac cttgtttccc aattagcaga tgttgatttc
   111361 tcttcaggga ttaatagaat aactcattat gaccgttata aaactgttat ggtcaccggc
   111421 gatatagcaa gcggatacca tctaagcgat ataacttctc aaattaatga acggatacaa
   111481 aaattcaatt ttcctagtgg ttatcgtttt gaatggggtg gagaagcaaa aatgatgcaa
   111541 gaaacaacag ttgatatggt tcgcaccttt attcttgctg taattttaac ctatatgtta
   111601 ttatcagcaa ttctggagag ttttgcacaa cctgttttaa ttttggcaac agtccctctg
   111661 gcattaattg gagtagttct ctccttactt attgccggac aaacatttaa catcgtttct
   111721 atgatgtcta taataatgtt agttggcatt gttgttaata acgctatatt aataatggac
   111781 tatgttaata ttaaacgcag ggatggatat agtgtccacg atgctttaat ggaagcaggt
   111841 aaaatgaaat taaagccgat agttatgtct accttggcta ttatatttgg tatgttacca
   111901 atggctatag gtattggtag tacaggagct gaaatgacca gaccaatggg aattgtatcc
   111961 attggaggat tggtagtttc cactttcctt actctaataa ttattccggc tttctatttc
   112021 ataactacca aaaatatcca tgctaaaaaa gaatcccagt aaaaaaggag cgtacaatga
   112081 aaagaatcac acgtctcatt agcctcgctt tattagcgat atttatcctc gtcggtatct
   112141 tcggatgcta tggcaatatg tcactaacca aaaaagtgta taactttaat ggcagtttag
   112201 gtaacaaata tgtccagagt attgttaact gggctttttg gatatgtcca gtttatgaag
   112261 catctatgat tctggacatt tgtttgttta acaccattga attttggaca ggcagcaatc
   112321 ctttaactat gaacgaaaat gaaaagataa ttaaatatgc tgaaggtcct aatggtgaat
   112381 ataaattcga aatcagtaaa aaccaaataa ttatttctca acccaacgat ggcactggta
   112441 atgaaaacac agttctttct tttgatccag atagcaatag ttgggttatg aataatgatg
   112501 gtgtgtctca acaaattggc tatctggaag gtgatcagct aaagctagtt tctccttctg
   112561 gagaggaact atcaattaat ctcataaaat aaggaaatac agtgatagaa cgacaaaaaa
   112621 tagaaagcat actggataaa gttcgccctt ctattcaagc agacggaggt gatgtcgaac
   112681 taattaatat ccgtgacgat aatgttattg aagtacgttt gaagggtacc tgtaacggat
   112741 gccctatggc aaccttaact ctgaaagctg gtattgaacg tatcattaag gaagaagttc
   112801 ctgaagttaa agaagttatc gcagtttaac aaaagtaacc atccctgata cttgcaacat
   112861 acataaaatg ggtgtggata tccacaccca tttttatctg tgggatttat agcttttcag
   112921 tatctccttt ttttaattta gtgtcaattt tttaggacgc ctcagtacgc ctcatagctt
   112981 cctatatcct tcccttataa ttcccatatc attcccttat cgatccctta tgacaatatg
   113041 gaaatgatag tggaaagtct ttagcagtat atcactgtta agtagtagat tacatataaa
   113101 aaagaaggtt attgaactgc attgatggca ttaatactat cttggcagaa tgaaatcaga
   113161 aacaaaaaaa ttgacaaggc agcgcatttt aaaaataagg taataaaaga aattataaaa
   113221 aacaacggag ttcaattatg aaaaatattc tggtgcttgg tgcagccggt caaattggat
   113281 cagaacttgt tccttactta agaaagattt atggtgcaaa ttctgtagta gcagcgtatc
   113341 ggagtactcc cttaacctta gaaatccagg aaggtggtcc ttgtgaaaat ttggatgcaa
   113401 tggattctaa gaagttattc gaagtagtaa aaaaacataa gatagattct attatcaatc
   113461 ttgttgccgt tctttcagct aaaggtgaaa ataatcctct tatggcctgg aacattaata
   113521 tgggaagctt gataaactct ttggaagtga tgagagaagt gaagggtgca atttttactc
   113581 cttcctcaat tggagctttc ggtccttcca ctcctttgga taatactcct caagatacaa
   113641 ttatgaggcc tactaccatt tatggtattt caaaagtagc tgcagaactg tggggagatt
   113701 attattattt aaaatatgga gttgatgcac gcggagttcg ttatccaggt attatttcga
   113761 atgtaacttt acctggtggt ggaactacag attatgctgt agaaatatat tatgaagcta
   113821 ttaaaaataa acgctatact tgctatctta aacccggtac atatttagat atgatgtata
   113881 tgccggatgc cttaagagct tgtgtagaat taatggaagc cgatcctaat aaattggttc
   113941 atcgtaatag ctttaatgta acagctatga gctttgagcc agaaatgatt gctgcagaaa
   114001 ttagaaaaca cattccagaa tttgaaattg attataagat agatcctgta aagcaatcaa
   114061 ttgcagatag ttggccaaat tatatggacc aatcagctgc tcaacaagaa tggggttgga
   114121 aacctgaata tgatctttcc acaatgactg tggatatgtt aaatgtcctc tccaaaaagc
   114181 ttaagtaatg gttaaaatag gagttgtggg agttggccat ttaggtcaat atcataccca
   114241 aaaatatcag agcattgata acgcagaatt agttggtgtt tatgacacta acttcccaag
   114301 agcccaagaa attgctggta aattaaaagt ccattgtttt tccagttatg aagaaatgct
   114361 cgctgtttgc gatgcagttg atatagtggc tactacaaca gcgcattatg aattagggaa
   114421 aaaagcatta ctttctggaa agcatatttt tgtggaaaaa ccgataacca gtgaattagc
   114481 gcaggcacag gaattattag aaattgctgc agctaaaaat ctgttgatcc aagtgggtca
   114541 tattgagcgt tttaatccgg ttattttaaa gacggaaaac gagatctccg atccattgtt
   114601 tattgagtca caacgaatat catcttttca acctcgggga acagatgttt ctgtagtgct
   114661 cgatttaatg attcacgata tcgatttggt attaagtttt gttcagagtc ctgtatctga
   114721 gatcagagca agtggagtgg gtatttttac tcctacgatt gatatagcca ctgctcgcat
   114781 tgaatttgtt aatggagcca tagcaaatgt tacctcttct cgggtatcga tgaaacagga
   114841 acgcacctta agatttttcc agagagatgc ttatgtgaaa atggactttt tatccaaaca
   114901 ggttagaata atcaaaaaaa gcgataatgt agataaatta ttacctctaa tcttgagtgg
   114961 agttgttgat atcaatccgg aacagttgtt agatatacaa aattacgatg ttggtgatac
   115021 tgaaaaagat gctctaactt tggaactgga atcttttgtt gattgtgttt acaataacaa
   115081 aaagccgata gttgatggct tagcgggaac tagagctttg gaaatagcta ttaaaataac
   115141 aaatcagata caagctcaaa atgccaaatt gagagcttga ataataggta gaaaaatgca
   115201 agatgtattt ataaaagata tcgataaata tatagataaa caagttcggt taaaaggatg
   115261 ggttcgcaat atccgtcata gcgggaaact actttttata attcttcgag atggaactgg
   115321 cgaacttcaa actgttgctt tccaacctga tttaggtgat gagaaatttg agttagcaaa
   115381 acggcttact ttagagtcgt cggtaataat ttgcggcatt cccaaacccc atcctaaaaa
   115441 agagaatgta tatgaattag ccgtaagtga tgtaatgccg attcaaatag cggatgaata
   115501 tccaattagt aaaaaagaac atggtcctga ttttctgctt tccaatcgtc atctatggat
   115561 aagatcacag aagcaatggg ctgcccttag aattcgtcat tctgtttatt acggtatttg
   115621 taactacctg aatgagcaaa attttattcg ttttgattcg ccaattctta ctcccaacgc
   115681 ttgtgaagga acaaccactc tttttgaact tgaatatttt gatgaaggaa aggcatatct
   115741 ttcccaatcg ggtcagcttt atctggaaac aggaattatg agtttgggca gagtttatga
   115801 ttttggtcct gtctttagag cagaacgctc taaaactcgt aaacatttaa ctgaattttg
   115861 gatgatggat gcggaagcag cttatgttga acatgaggaa aatatgcaaa ttcaggaaga
   115921 gctaattcgc tatgttattc ggtatgtgct ctctaattgt aaattggaat tggagatttt
   115981 agaaagaaat gtggaagctt taaaagcagc tgatgcaccc tttaaaagaa taacacacta
   116041 tgaggcagtt gaattcttac gccaaaaagg tagcgatata acccataata aagatttggg
   116101 tgctcaagat gaagttctgc ttacagagga ttcagcagtt cctgtttttg tggaacgctg
   116161 gccacaagag atcaaagcat tttatatgaa acgcgatcct gaaaatccaa acctcgtttt
   116221 gggagatgat ttaattgctc ccgaaggttt tggagaattg attggaggca gtcagcgcga
   116281 agatgattat gatttattat attcgcgtat ggtagctgaa aaaatgccca ttgaggatta
   116341 tcagtggtat ctggatttgc gaaaatatgg ttctgtacct catagcggtt tcggaattgg
   116401 actggaaaga ttagttgctt ggataagtgg tgttcctcat atcagagaga caattccctt
   116461 cccgagaatg atttacagaa tttatcctta aaaagtctta gctggtagtt ggaagtttaa
   116521 cagccggaaa agatcaacac aaatgtacta gcggaatttg aattctaaag cttattgcag
   116581 gtgtttagtc cataatccta aatgattttg aataaaaaaa ataacaagga tagaaaaatg
   116641 attacgaatt acttttctga aacaacgaaa acgatgaaat catcgctgat cagagaactg
   116701 gttgcggaaa ccaaaaatgt tcccggctta atttcttttg caggtggctt tccttctccg
   116761 gcaacttttc ctaaagacat tttaagtgaa ctttacaagg aaatagttgc tcagcaagga
   116821 agagatgttc ttcaatatgg taatagtgaa ggtgatgaca ttttgaagga tgaactttta
   116881 aaatgggaaa attatcccaa tttgaacaga aataatatga tcatcactgt aggagctaca
   116941 aatgctatat attatatgac ccgctgtttg atagatccag gtgatgtaat tctttgtgaa
   117001 gcccctactt ttttaggaac aattgtcgct tttgaagcta ttggagcaga agttcaaagt
   117061 atcccaatgg ataatgaagg aatagattta aacaggatgg cagaaaaagt taagcaacta
   117121 agagatgcca ataagaatat caaattcctt tacactatcc ctgattttca aaatcccgct
   117181 ggaatcacaa tgagcattca gcgtcggcgt gatttgctaa atttctgtga agcagaagag
   117241 attctaatta tggaagataa tccctattcg cgtttacgct ttagtggaga agaacttcct
   117301 accctatttc gtattgcaca tacagaaatg aataattctt ggctggtaac cgaaatcgtt
   117361 tcattttcta aaatcctagg acctggaatc agggtagctt atgtaaaaag tcacccagag
   117421 ttgattaata aaatggtctc ctggcaacag aaagttacta tcagtccaga ttgtgtaact
   117481 gaacgggtcg tagcaaaatt catcgctgat ggctatatgg aaccacatat taaatctctg
   117541 tgcgaattct ataaacccta tttggataaa atgcttactt gcttggaaac agaaatgcca
   117601 agttatgttc agtggacaaa acccaagggt ggaattttct tgtggttgtg gttgcctgaa
   117661 gatgtaaatg ccgatactct tttctataaa gctaaagatc acttggtaac cttcattcca
   117721 ggaagcaaat tctatcccga aggtcaagaa aaatttaact gtatgcggct caactatacc
   117781 tattccactt ttgaacaaat cgaaaaggga attaaacatt taggtgagct agtaaaatca
   117841 atttatgttt gacatcggta cagttcttgc ttatattatt aagtgatatc ttcttttaag
   117901 aaggggaaat aaaaatgaaa aagctgatat tgattacaac cctgagcatt tttctgctgc
   117961 ctttgatggc acagatagaa tttccgcgtc ccaatttgaa tccttattca agtaatgggg
   118021 gaaattcgct tctcaattta gagaaactat caatgagtca ttcaatgggc tttgaagcag
   118081 gaaccagctc taccggatat ggctattatt tatcccgcta tacaaatcac ctgaaatata
   118141 actttaatcc caagctggag ctcgatttag accttaactt tgtaaatgtc ggtggtttca
   118201 atactagtag caaatttgaa tttaataatg acaatagtag tagagttata ccggaatttt
   118261 cattaagtta taaaccacga gataatgtcc aaattcaatt ccagatgctt tatgggccat
   118321 atttaaatcc ctataaagat agctggtata gaagttggta aggagattca actgttaaca
   118381 ttcgtaataa taattatagc tgtccttggt gctgccttgg gcagcttttt caatgtgcta
   118441 atttaccgca ttcctaaaaa gcaatccatt atattccctg gttcacattg tgaatcatgt
   118501 aagaaagcta ttcccatcta tcaaaacata cccattatca gttatatttt gctgagagga
   118561 aaatgttcaa ggtgtggagc taaaattcat tggcatcact tattggtaga gattattacc
   118621 cctttgttgt ttttgggttt attctttcgc tatggcttgc acgatttccg attttataag
   118681 tatatattat tggcttcttt tctgattccg atctttttca ttgatgcaat aaatcagatt
   118741 attcctcatg tgctatccat tccattgctt atatcaggta ttattttagc aataatccct
   118801 ggaaatgatg ttggcattgt taatgcttta ctaactggag taattgtctt cagtttattg
   118861 ctattactgg cttgggcgta tcaaaaatta cgcggagtgg aaggtttggg aggaggagat
   118921 atttggttgt taactggttt agcaatatat tttggcttga ccggtatccc ttatatcttt
   118981 ttactatctg cattgatggg gattatctat ttcctcatat ttgtgaagga taagagtaaa
   119041 ccatttgcct atggaacctt tattgctgtt gctgcaatta tctggtcagt gattggagaa
   119101 gatacaattt ttaacctatt gagtttttta taggggctag ccgcatacag atactcatat
   119161 tcaaatttga aagatagtgc gtaaaaagtt tgcgattacc gctatgtaag agaagagtca
   119221 tcattgccaa aaaagctgta atgcttatta caaatttatt tgacagtgat gactcataat
   119281 ttgagcgact tattgcttat ttgatttgca tcgcagtgtt ttttcttctt gggagtttat
   119341 attctcaaac atatttattc atatcgtaaa gcatctatca gattcaggtt tgccgcttta
   119401 gatgctggat accatccaaa gaagatacct atagccatcg aaaaacctac tgatattaac
   119461 acggaaaaag gagttacagc gatgctccaa tccatagcat ttccaactat tgctgaagct
   119521 ccaaaaccaa gaagtattcc aataaaacca cctaaaatac taatagcaac tgcttcaata
   119581 ataaattgta ataaaacatc tctttttcct gcgcctacag ccatcctgat cccaatttct
   119641 tttatacgct ctgtaactga aaccagcatt atattcataa ttccaattcc accaacaagt
   119701 aatgatatac ctgctatgct tgctaaaagt atagtcattg tatctgatac tttactggca
   119761 gtttcagcaa tatcagtttg actcctaact aagaattctt cgctggttgt tccgcgatgt
   119821 ctgttttgta gtaattctag aatatcttgt tgcacttcgg gaattctatc tttggaaaca
   119881 gccgacacaa caatcatcat atttctccat ctacgaccca ataatcgagc atagacagtt
   119941 gtataaggtg cccaaacact atcatcctga tctccaccca tcacattttg tcctttggat
   120001 tccatcactc ctttcactgt gaaagggata ttgcgaatac gaataatctg tccaactggg
   120061 tctatatctc caaagagatt atcagcaaca gttttcccga ttacacacac tttagttccg
   120121 ttttcaacat cgctatcgta aaacaattca cccttttcag tattcatatt gcggatgtag
   120181 aaataatctg tatctacacc cataatgcct gttctccaat tctgggattc aaatttcaat
   120241 tgggcaaagc tattataaac aggtgaagca tataaagcac cttctacttg tgatcgaata
   120301 gcaaaaacat catcaagttc aagtaatgta ccacttccag ctgcttgacg aacattagta
   120361 gtgcttcgtg taaaattaac tgtaaccata agtacatttg tgcccataga agatatttgg
   120421 tcttctacta tttttttggc accttgccca atggcaagca tagttatcac agctgccaca
   120481 ccgataataa ttcccagcat tgttaaaaaa gttctggttt tatttctagc aagcgatttg
   120541 aaagctatgc gaattatacc tattatactc attcttcatc tccaggtaat ttaggaaggt
   120601 gggctaagtc atattcagca ttttgaggat ttttattggg aacatcagat attatgcgtc
   120661 catcacgaaa agtaatcaat ctttgtgcat accgggcaat atcattttcg tgggtaacca
   120721 gaaggattgt ctttccttct ttatgtaatt tctggaaaat agtcataact tccaaacttg
   120781 ttcttgtatc caaattgcca gttggctcat cggcaagtaa aaacatagga tcattcacaa
   120841 tagcacgggc aatagcaact ctttgttgtt gcccacctga aagctgatta ggaaagtgtt
   120901 ttgctctgtt agcaatatta acagtagtta gtgcttcaat tgcttttcta attctctggt
   120961 gatgagacat tttatttcta tatagcagag ggagttcaac attttccaaa gcagttgttc
   121021 tgggcagtaa atagaaattc tgaaacacat aaccaatcat ctgattgcgg attttggtta
   121081 gttcagtagg agtgatttcg tggatgccta tattatttat ataatattca ccgctggtag
   121141 gtaaatccag acagccaagc aaattcataa gagttgtttt gccgcttccg cttgctccca
   121201 taatggcaac aaattctcct ttatttattt ccagagaaac acctcttaat gcgtgaacct
   121261 ggatttctcc catctgatat gttttaacta agttttctgt tctaatcagt aaatctgaca
   121321 tattagaaag gcctcattcc aggaccacct tgcgttccat tagtggtatc tgaattcttt
   121381 ttactattca ctccggttat caatacttcg ttaccgttaa gacctgaaat aacttcaatg
   121441 aaaccacttt cggaaattcc agtttttatt cctttagctt ttggaacatc attttccagt
   121501 acccagacaa gtgctaattt tccagaactt acattattag ctgatccttt ttgagttctt
   121561 tttccggatg tattcttgct taccttaatt gaatctgtat tactggaact ggacattgca
   121621 gaagccagag aagcataagc ttctttttgg gcttgtttga taagatcatc actccatttt
   121681 aacccaaatt gttcccaaac ttctttactg ggtttgaagc gtgtagctgc ttctgaaatt
   121741 cgcatcacat tttctcggga ttgaataata atagttacat ttgtagtcat accaggaaga
   121801 agtttttgtt caggattggc tgcatcaata atgacgctat aggatacgac attggattcc
   121861 gtagttgggt ttaagcgaat ttgcttcacg gtaccggaaa aattttcgct gggataggca
   121921 tccaccgtaa attccacagg caaaccaact attatttttc caatatctgc ttcgtccaca
   121981 ttagctgaaa tttgcatttg atcgaggtta ttagctattt gaaataaagt aggtgaactc
   122041 atactggctg ctacagtttg tcctgcatct acatcacggg aaaccacaat tccatcaata
   122101 ggactggtaa tatatgcatt agccaagttt ttttgagctc gctgtaaagt taattgggca
   122161 ttattataat tctgttgtgc agtttgataa gcatagaggg cttttttggc atcatattca
   122221 ggggtcattt gtttttccag aagttctttt tgcaaatcat aatccagttt tgcttgatct
   122281 aaagcagttt ttgcttttgc caaatcagct ttagcagctt ccagattagt agaaaggata
   122341 tccgtatcca gttttgctag taattctcct ttctttactc tatcgttaaa atctttgtat
   122401 agtttatcga ttttgccact aacttcggtt cctacactaa ctaaaacata aggatttagc
   122461 gatcctgttg cagttacgac ttcttttata ttaccaaagg aaggggtaac agttctaaat
   122521 tcaggtttgt tttttgcctt gcgcatactg gatatcacaa tgatagcaat aattataagc
   122581 gcaattataa tgatatactt gagatatttt ttcattagtt gtccttgatt tttatcttat
   122641 aggttttact ttaccatttt cctaaaactg gttcagaaag cagataattt atctcttcct
   122701 gttttgccat tattgaatat agattgttat tataggagat atcggtttca atataattgg
   122761 ttcgtgtttt atctagttcc aaaagttcaa ttagacctaa acgatagcgt tcttctgcct
   122821 gggtaatttg atctctggat tgagccagtt gttcactgta aagttcattc attcttttca
   122881 aatattgcaa ttctcgtaaa gcactatcat agtttctttg taactgatcc tttttttcat
   122941 caatgcttac ttgggaaagc tgacgactga tttttgttct agttctgctt tcagaatttt
   123001 caaataaatt caggagagga tagcttagcg aaagagatat tccatgatta gtgttgtaac
   123061 ggtcaaaatc aaaatcagca ccacttactg tacggtcaaa attataagcc aaattaagcc
   123121 ggggaaagtc atccagtttg ttttgcttta attgcaagtt cagtcgtttc aattcttgat
   123181 ttaaaatttg aatatcggca ttattttctg tattgaaagc aggaagctct ttttggatat
   123241 ccagatccag ctctgttaaa ttataacctt catcttgcat ttgtacaagg gcaaaaagtt
   123301 cctttcgtgc attagcgatt gtattatcca attgcattaa agctatattg gaatttaaaa
   123361 cctctatctc attctgctta acttcaaaag gagtgatttt tcccaattgc aatagtattt
   123421 tgctttgttc ccaaacccgg gtttgaataa gaagattttc ttccagagaa ctgcgttgtt
   123481 tttgtgaact tagcactttt atatatgcgc tgaatacatt atatgcataa gttctgcgag
   123541 tttgttccag ctgcatattt gctgttttcg tatccaaaga agcatataga taattaaaat
   123601 aagctgcatc gtttaaactg atagttttac ttatggataa accagcagaa ctgcttacat
   123661 catcagttcc agcggcagga tcaaaatctt tatttactcc tccagtaaca gaagcatccg
   123721 gtaataaatt ccattttgat gtattcagca ttgatcttga gctttgataa cttagagagc
   123781 tcttttgaac actgaaacta ttctccagtc caaaacttat cagctcttcc aaagtgtaac
   123841 tttgggcagc taacaatagg ggcagcaaca ttagtataac tgtgataact ttcatttttt
   123901 tcttttttcc ttgattcctt acaatttcct tatctgtatt ttgtatctcg tttcttttaa
   123961 ttataccatc ttatgaagta tatcctgcaa ctttttttat atctacagac aaagatttct
   124021 tttttacctt ccaatatttc tctctatact cgtcaatagt tttcttccag actgcaaaaa
   124081 cgaagaggat aactttttcc tttatatcaa tgcttccatt ctttcatagc cattcacttg
   124141 gggaagaaca cctgcttcaa cggctttttt tacacttata cagggtaaat tctcttgata
   124201 tatttgttct ctggttatcg ttttcaggtc tatttccgga tagagtttag ccatatttgc
   124261 cattagcagg tggtacaaat ttacccaaga gcgtagctgt ttatatctat ttacttttaa
   124321 tccattgtaa cgcaaaagat aaagccgcag atcattcttg tatacctctg aaggctgtag
   124381 ggcaaatatg ttgcgagata tattagcaat tcgagcattt tcttcggtta agcgaggata
   124441 tactctcttc cttgcgtaaa ttttcccgga tattctgtca taacagaaca ccaaatcatc
   124501 agctttaccg gtgtatcctg acaggccata tttgaaattt actttcattt tgttttctcc
   124561 tatttcgtcc ttctatatat atagggggtg acaaaagtgg ggaagtggtg acaaaaatcg
   124621 tgtttttttg ccatcaaata aaaagttctt tcgaacatac ataccggact tacacagccg
   124681 atatgattcc catatccttc ccatatcgga taagggaatc atatgggagt gtttttgctg
   124741 gtatattggc tatgattaag tttccatact aaatactctt aggagaattg attaactaac
   124801 cctgggagaa gacagttcat aaagccccat aaccccaaga aaagagaaac tcattgacag
   124861 ttaagccata cttttaaaaa aggataacac tcgcttagaa tgaaaaaccg ttttttttta
   124921 cttaaagagt ctttagcgat aaaaccaaat actaatataa cataaaagga gaccaaaatg
   124981 gctttcaacc ttaaaaaccg taactttctt acccttatgg attttactcc taaggaaatt
   125041 caatttctgc ttgatctgtc aatggagctt aaaaaagcca aatatgcagg aactgaacag
   125101 caaaaattga aaggaaaaaa cattgctctt atctttgaaa aagatagcac acgcactcgt
   125161 tgtgcctttg aagttgccgc tttagatcaa ggtgctcata caacatattt aggcccaact
   125221 ggttcacaat tgggcaaaaa agaatcagtt gccgatactg caagggtttt aggcagaatg
   125281 tatgacggaa ttgaataccg tggcttcgga caagaaattg tggaagaatt agcccaatat
   125341 gccggtgttc caatttggaa tggtttaacc gatcaggatc atcctaccca ggttttggct
   125401 gattttttaa cagctatgga acatttaaac aagcccttga atgaaatggt tttcgtttac
   125461 tgcggagatg gacgcaataa tatggcaaat gctttgatga ttggtgcttg caaaactggt
   125521 atggattttc gcattgttgc tcctaaatct ctattcccag atcaaaatct ggttaataaa
   125581 tgcctggaag ttgccaaaga aaccggtgcc aaaatcgctt ttactgataa tattgacgaa
   125641 ggtgtgaaaa acgctgatat catttatact gatgtgtggg tctctatggg tgaaccggat
   125701 agcgtttggg aacaaagaat tactcttctt aaaccctatc aggtaaacag cgaaatgatg
   125761 cttaaaactg gtaaaagtac aactatattt atgcattgtt taccttcttt ccacgatctg
   125821 aaaaccaaaa ttggcaaaga tatttacgac aaattcggct taacctgtat ggaagttaca
   125881 gatgaggtct ttgagggacc ccaatctgtg gtctttgatg aagctgaaaa cagaatgcat
   125941 acaattaaag cagttatggt agccacttta ggatcgtaaa ataaatctaa atataaatat
   126001 gggagcggga taacataata cctgctccca ttttcgtaat aatactgttt atttcaattt
   126061 caagatttta cgggtttcta ttgtattgcc ctgatttgcc ttaattagat atattcccga
   126121 ggcagtttgt ttatctttat agtctgttcc gtcccaggaa attttatttt tggaatcagc
   126181 aatgcccttg taaagagttt taactagctg acctttcaga ttgtaaatct taacttccag
   126241 tggggcactg atgtttttac agaggatatt aaactgcgtt gtactagaaa aagggttggg
   126301 ctgattgggt aaaatagaaa tttcggggac ctgatttata acttcatcgg aaactgatat
   126361 tggttgttca aaacaggtct ctacgatata gcgcaaaacc tgacggcttt ggttgaaatc
   126421 cattgagtat aagggaaaac ttaaagttac tgttttacct gcataataat tgtttaggat
   126481 tccaactact cctccattta aaacaccttg tagggtatta tgatcataag cggaagcata
   126541 actataaatc ggagtagctt gagcattcgg gttgataact tcaatatgaa ataaatgacc
   126601 attgagacca ggtggaactt tagcaggatc aacatttact tcaatataat tgggatcagt
   126661 acttaccgct ttgttgaaac gcgcacttgt actatagtct acattgctta ttcctagaac
   126721 ttcatttatg aaagaaccag aaggaaaaga ggcaggataa ccactattca tttcaaaagc
   126781 catactggga tgataaagag agaacagaac ttttccacca taataaatat agcttttaag
   126841 agcatcctta atgtaatagg gatactgcat atcggtataa tcgttgccat gccagataat
   126901 tacggaataa atacccagat cacacagacg caaggtatca gttaaagttg ctaaatcgaa
   126961 atgagatgtg ctccataaac cagcaaccaa ttgatcataa aagctatcaa cctgttcatc
   127021 cgttggttga aaaagattgc taccgctaaa atccattgtt tcatcaacaa ttaaaacacc
   127081 gtaattcata tttataggtc tggtttttat cacagcagta taaggactgt tattattatc
   127141 attatcaaaa gcacagagtc gatagtaata ataaaaatct cctccttgaa cattgttatc
   127201 cgtaaaactt tgaacagtgg gaggtaaagt ggcaataagc tcaccgattt ctgagctgtc
   127261 caaagaacgg tataatttat aacctgctaa atcaagctcc gtatttgctt gccaaacaag
   127321 ttcaactacc tgaggcaaag gatgatctgt aaaactaact gggacggaag gaattaatct
   127381 gggaacagct tgattaaata taggcatact ttcattatca ttatcatcaa cggcagttaa
   127441 agcaaagtga taaagctggc cggttgtcag attgttaata cttatctcat tctgaatcgt
   127501 ggtttgagaa tcgctaagag tatcctgagt aataccccag gaaattttat aatgcgaaag
   127561 agtaggatcg gaaactggat tccaactaag atgaatactg ctaccggtgc cttcatcctg
   127621 aacagtaaaa tttgtaggcg tggagggcat atcagcaaaa ctgacagcaa cggcggtgga
   127681 agcacgaatt actttggtac aataaaatgg atcaagatta gctacaatat cattattact
   127741 gtgatagaca tccgaaaaat tatactcaaa aaagtaaacg accgggaagc catgctgcca
   127801 gaaggaataa ctatcactgg cgcttaaatt catcgatcca taaacaggca atagagtggt
   127861 atattgtgaa gttaacatag ctgcatattc ggtgagatca agatatccat cataaggcat
   127921 taaaaaaact ctcgaatccc agggattggg acttatatta gctatcatat catgattaat
   127981 cattaaacgg atatccagat tattttgatc tgccattgta gcgtatgcct tagaacccca
   128041 aagcccaaat tcttctgccg caaaagtaac aaaacggatg gaacatttgg gctgataatt
   128101 atttgccatc attactcttg ccatttctaa agcggcaact gtgccactgg cattgtcatc
   128161 tgctccggga gcaaaagtca ttggattatc gttagttatg gaatcgtgat gtccacctac
   128221 gataatataa gtatccggat aaatagttcc cggaatggtt gctactacat tatattgagt
   128281 ggttccactc caattaaaag gatataattg agaatcggtt atcccaaatc gttcaaattg
   128341 agccttaatc caattagcta cctctaaccg attatttgcc aaagcataac gagtcataaa
   128401 atcttgtagg gattgaatga aatacgcaac actgtccaca gaaacatcag caatcatatc
   128461 tacaatatct gtcctggtat gtttgagtga attatttaga gttgaatagt ctgctatttt
   128521 aatgggttca tataacaaag gaataaaagg atagattatc tgctgtcgca gctggatttc
   128581 atctaaatct gttttcagta agacaaccga acccatcttt aataaaacct ctccaactat
   128641 gtttcgatca aaatcgggat gcctattacc aattgtaacc agataaaatc ttccgtcttg
   128701 aggagttccc aaattaaccg ctttggggta attacttaca ttggtgcctg caataatata
   128761 gaactcgtta tagtaatata tttccagatc ctgcgctgaa agttctttaa ctttattgcg
   128821 taaggaattc tcttctttgg ataaggaaag ttctgaagca ggaattccga taataaattt
   128881 aggtaaggca tttaacattc ctgctgctaa tataatcagc agtaggctaa acatttttct
   128941 catatttatc tcctaagtat tagaatgaaa taattgaagc atatcatatt ccatcttttg
   129001 gttacttata tattaatctg tatagcgcat aaggcaaata aataacacaa atttctattt
   129061 tgctactcaa aaccttgtga caatgtaggt tagccctatt tcaaggattt ctttttgctt
   129121 tttttatttt tttcttcttt acttattgta gggttatgtt ttttttggtt tcggcttcaa
   129181 tcttgcatat aatatatagg taataataaa tgatttaatt agctaagaaa aatagggaga
   129241 aacaatgaaa aaaatttcat ttatgctcat acttgttttt gctgctgcgc tcttaatggc
   129301 agtaagcgga actatcaaag tcaatgataa tccagccaaa gtgcaattgc tggaaaacaa
   129361 caagaatgga ctttctgtcc attatgctct ggacgaaatt caattcagag acattaatac
   129421 taaagaaggc atatttacag agcttatttc tactaattat acttctacaa atacaacagg
   129481 tttacctgcc ttgcctttaa tgcggcaact tatcagtgtt cctttaggag ctgaagttag
   129541 tgcttatttt acaactatta actctaagac cataaatctt accgaacaag gaattaacta
   129601 tcctctgatg ccccgtcagg aatctgtttc caaatctgct gatatagaac aacttccatt
   129661 tattgtaaat cgtgattttt acaattccaa tacttggacg gataaccctt ctattagtgt
   129721 aaccgagctg ggaattatgc gtggaacaag aatttttgct gttgattttg tacctgtaaa
   129781 atacaatccc accttaaaac agatagaagt tatttataat gcagaagtta aagtaacttt
   129841 cagcggtgct gattatgctg ctacatcaga attacgcgat aaaacatatt ctgctgcttt
   129901 tgaaaatgtg tttgctaaca ctcttctaaa ctattatgaa gaaccacgct cttcattact
   129961 tcgctatccg attggatatg ttatcattgt tcctgataat tttattagtc ctatgcaacc
   130021 ttttattgac tggaaaaagc aagaaggcta taatgtaatt gtagctccca cttctattac
   130081 tggaacttct acaactacaa ttaaaacata tatgcagaat ttatggaatg ctgctacaac
   130141 tgaaaatcca gctccttcat atcttcttat agtaggtgat attgctcaag ttcctactaa
   130201 taatggaagt acaagttctc atccaactga tcttccgtat gttcgtttac agggtaatga
   130261 ttatttacct gaaatgtatt ttggtagatt ttccgctact actgttgacg aagttaccaa
   130321 tcaagttaat aagactctta tgcatgaaca atatactatg cctagcgata cctatttaaa
   130381 tgaagtagtt atgattgctg gtgtggatag taattggtca ccaactcatg ctaatggcca
   130441 aataaactat gctactaata actattttaa tactgctcat aatattacca gccacactta
   130501 tctttatcct gcatctggaa attcagcatc aacaatcgtc cagaatgttt ccaatggaat
   130561 tggttatata aattacactg ctcatggaga tgtaacaaac tggtctaatc caagctttac
   130621 aattagcaat gtaaattctt tgcaaaatac caataaatac tgttttacag ttggaaactg
   130681 ctgcctaacc aataaatttg atacagccgt ttgttttgga gaggcgtggt taagagcaaa
   130741 caataaaggt gcagtagttt atattggagg caccaattat acttattggg atgaagatta
   130801 ttattggggt gtaggttata agccacctat cgtaggaact ggttctcctt ttgtggcagg
   130861 tcatacaggt gcttatgatg ctctctttca tgatcataat gagccttttg ctgattgggc
   130921 tggaaatgca ggaagcatgg tttttatggg aaatatggcc gtagttcagt ccaatagcac
   130981 tcgtgctaac tattattggg aaatctatgc tattatgggt gatccttcta taactcccta
   131041 tcttggaatt cctgctcaaa atgcatataa tcctcctgct caaatgtttt tggggcttaa
   131101 cagtatggat attcagacag atccctatag ctatgtagct ctttcaatga ataatgaatt
   131161 acacggagta ggtttagcgg atgctacagg aaaccttact ttaaattatg ctccttttac
   131221 tgaacctgga atggcaaaac tggtaattac gcgttccttg cgtaaaccct taattgccga
   131281 tattgaagtt attcccaatg aaggacctta tgtaacagta ggtacattgg aattaaatga
   131341 tggtaataat aatatagcag aagcaggtga taacatcaat atcactcttc aattcaataa
   131401 tgtaggtatt gaagcagcta ctaatctaac tgctacatta acctgtgaca gtcaatggat
   131461 tatattaaat aatgcaactg ttcagcttcc tgatattact tcaggaaaca gtattactca
   131521 aaccaatgct tttaatgtta atattctgcc taatgttcct gatcaacata ttgctgcttt
   131581 tactatcact gttacagatg gaactgatac ttgggaaact cagcgcaatt taactattaa
   131641 tgctcccaat gttgtttttg gaaattatac tctatttgat ggtgatggag atggaattta
   131701 tgaaccaggt gaaacagtaa cagttactct ttccatatcc aatacgggtc atatgaatgt
   131761 agatagtggg tctttagcaa tagtgatgaa taatccttat gcagctttag ataattatgg
   131821 atttatcatt cccccgctta cagttggaaa tggaatacca attgttttct acttgtttat
   131881 cgatcagaat tgtccagaag gcgaaatgat cccaattgga tttgctatga atgcaggaat
   131941 tcagatgtta aatcatagca ttattatacc tgttggaata gtaggggaga gttttgagag
   132001 ccaatctttt acttcatacc cttgggtaaa tgacagtgcc aatccttgga ctatagtaag
   132061 tggttcaaca aatgttcact tcggaaatta tgctgcaaaa tctggagcaa ttggtaatta
   132121 cggatctact tctttacaag tgactattga tatacctgaa gatggtgaca tctctttttg
   132181 gcgaaaagtg agtagcgaag ccacatacga ttgtctaaaa ttctatattg acaataatga
   132241 aaccggttcc tggagtggag aagttggctg gtctggagtt agttttcctg ttacatctgg
   132301 caaccatact ttcaaatgga cctattccaa agatgtatca caagttggag gaaatgattg
   132361 tgcctggtta gatgatatac tcttccctgg aataggagta ttagaaactg ccatattcta
   132421 taccacaacc gaacagatag attttaatgc tgtgttacct aatacaacag tgagcgcaga
   132481 ttttaccttg cgtaatttag gtaatattcc aatgcaagga attatctcaa ctcctgtaga
   132541 attcgaatta agccagaatg gcaatccctt atctgatgat tatgcatatc agatcgaagc
   132601 aggtgaaacg caattcttta ctattagtta tgttgccgga aatacagttt caacaattca
   132661 tacgaatttg gtgattacta ctaatgatcc tacacattct acagtagaaa ttccggtgaa
   132721 tttgactcct cttgctaatg ctgataacaa tgctattcca attgttacta ctctacaaaa
   132781 aaactatcct aatcctttca atccagaaac aactattcgt tattccctta gtgaggctgg
   132841 ccccattaaa cttgatgtct ataatatcaa aggtcaatta gtccgcaagc ttgttaatga
   132901 acctaaagca gcgggaaatt atacagttgt ctggaatgga aaagatgaac agggaatgaa
   132961 tgtttccagt gggatttatt tttaccggat gcaaaccaag aactattcat ctactcagaa
   133021 gatgatgtta atgaaataat tattcgatgt ggcaggcatc cttatttagg gatgcctgct
   133081 ataaaataaa gggaaatcaa atgctacata attctttata ttttgaaatc aaacaaggaa
   133141 gtaaccaatg aaacggattt tattattctt atgtctaata ctcagcttta tagctataag
   133201 tgcggaacaa attatcgtta gtaattatgc taatgatata catttaacga acagcaatcc
   133261 taataatatg gagctggaat ttactttagg cagttttttt agagaagccg ttaatataga
   133321 tggagaacag tggtttcatc tctttttaaa gaaggaaggg ataaccttag atgaaggttt
   133381 gccacaactt cctgttttag caagaagcct aattattcca cctacagctg caatgcatct
   133441 gaatataaca aacagtgaac atatagaact ggctatgccc attgccccct ccaaagggaa
   133501 tataactcgt aatattgatc ctgctacagt tccttataaa tttgctgatt tttatcagag
   133561 cgaggaaagt tatcccgctg agattgctta tttaacggaa ccgtttatat taagagatta
   133621 caggggaatc acagttcgtt ttcaaccctt tgtatattat cctgcaacag gtattctgag
   133681 ggtatatacc aagctgaata tttcagttta tgctcaggga tctgatttaa ccaatgcctt
   133741 aacaagtcca aagtcctctt atagtcgcta ttttgagagc acctatcaga atatgtttct
   133801 aaatttcagc actgctaaat atccatcctt aaatgaagaa ggtagaattt tagttattaa
   133861 gcatagtatg tttgatactg caattcaacc ttgggttgat tggaaaagac agcttggctt
   133921 tactgtaaat gttgtagatg ttacagaagc aggttcttca gcaactggga ttaaaaattt
   133981 cattcaagca caatacgatc tgaatgacgg tttaatgttt gttcagctcg ttggtgatgg
   134041 acctcaaatt cctactctta cttattctgg aggtggaagt gatccttcct atgcactctt
   134101 agctggaaat gataactatt atgatatctt tgtgggtcgt tattctgctc aaacagcagc
   134161 tgaactggaa acacaggtta accgcagtat ctattatgaa agagatattg ccccgggagc
   134221 aacttggtta gaaaaagcaa taggaatagc ttctgcagaa ggtggtagcg gtcaaggaga
   134281 tatgggtgaa agtgatattg ctcatatgaa taatattcgg acagaccttc ttaattatgg
   134341 ttacactact gttgaccaaa tttacgatcc aggagcaaca gcagctcaag tgacaacttc
   134401 tgtaaatcag ggaagaggat ttattaatta tgttgggcat ggtgccgata cttattgggt
   134461 aactacaaat ttcaacaaca acaatgctaa cgctcttact aatgattata tgctaccctt
   134521 tattgtttca gttgcttgtg tgaatggaaa ctttgttagc caaacctgtt ttgcagaagc
   134581 atggatgcgt tctgtaaatg aaacaacaaa tgctcctgct ggagctatcg ctttttgggg
   134641 ttctacaatt aatcaaagtt gggctccgcc tatgcgtggt caggacgaag taaccgatct
   134701 cttagttggc aatttaaaat atcgggtggg tggtttactt tttaatggtg cacataaaat
   134761 gattgaagta tatggtagcc aaggtcttgg tgatgccaga acctggacca tttttggaga
   134821 tgcatcatta atgattcgga ctaaaaatcc tcaagtgatt actgctactt acaatcctgt
   134881 cttgttttta ggaatgagca attttacggt gcagaccata ccttttgcca gaattacttt
   134941 aacaggaaac ggaattgtat acggacaagg tattgcagat gcttcaggta actgtgtgat
   135001 aaacttagat gttataccag atcaaccgat ggatttagct ctcactattt acgcatttga
   135061 ctatcagact tatattcaga ctattcaagt tttaccaagt accggacctt atataatggt
   135121 aacggaaacc acttttaatg ataatgatga taatgtcttt actgctggtg aaaccatttt
   135181 aatgggaatg gctctttcca atattggtag tgaaattgct tctaatgtaa gtgtaactct
   135241 ttcttccaca gatcaatata ttactatcat caatccggta cttaatattg gaaacattgc
   135301 cagtggagat gcaagtagca tagaagattt tcaaattcag cttgctaata acattccaga
   135361 tgaacatctt gtagaattga cggttactat tgctactatt gatggcgatg tttttgaata
   135421 taatcgtagt tttatagttt atgccccgtt aataacttgg ggcgcattac aaattgacga
   135481 cagtactgga aataataatg gtagaataga tgctggagag aatgtaaccc ttacttatac
   135541 tgttactaat tctggtcatt gtaatgctag tgatatttca actgatatag taatagatgg
   135601 cgtaccatat cttatagctc caataatttc tactatagat aatcttgcta tcgggatgtc
   135661 agctcagtta atttataata taactttcag ctctcaaatt ccgatgggaa ctccagttca
   135721 gcttacagca atgacaatga tcggggatta tgtatttgta aatatctaca cagttatagt
   135781 tggcattgtt atggagaatt ttgattataa tttcagtaat ttcccctggg tttttgaagg
   135841 aggaaattgg actattgctg gaggtagtta taatggcact ccggcagcga aatcccctac
   135901 aattaatcat aattctagta gctcaattag tattaacttt gaatgtcctc aggccagcga
   135961 aatttctttc tggaaaaaag tttcttctga actgaacggt gattatttga aattttacat
   136021 caatggagtt ttaaagtacc aatggagcgg aactgacgat tcatggagtc aagttacctt
   136081 cccggttaat cctggaacaa atgttttcaa atgggaatat acaaaaaatg caagtataac
   136141 tcaaggtagc gattgtgcct ggcttgatga tattattttc ccttctacag gagaaaacat
   136201 tggtacaccc atatttgtta ttgatatcga aaacatagat tttggacaag ttacagttaa
   136261 tactacagcc tcacatgaat tcactatttg caattgtggt acagcatcta tgcttggcac
   136321 tatttctaca gaagaacctt tctctttagg gatgccttca atgccttctc aatatttgga
   136381 gtacattatt cttccaggag aagcatttac tttcaatgtt aatttccgtc ccactgaaaa
   136441 cattgaatac aacggctctt taattattca tacagacgac cccaatgcct taattaataa
   136501 cattcccctc tatggaacag ggaaaccggt tgccaatgaa gatcctgtta tcagtgttgt
   136561 aacttctttg catggttgct atccgaatcc tttcaatccc agcacaagca tcagtttcag
   136621 tttacatgaa aaaagtagag tggagttggt aatttataat atcttagggc aaaaagttag
   136681 aaccttagta aataaaccct tagaacctgg tgaacataaa gttacctgga ctggaacaga
   136741 taattcaggt agacctgttg ctagtggtat ttatttttat aggatgaaag ctggaaatta
   136801 ctctgaaaca aagaaaatga tccttaagaa ataagctata aacaagttag agccggagga
   136861 gtatattcct ccggcttttt gttttgctta tgtcgaattt taattatgta aataaactga
   136921 taatacaatc gcattataga taaaaaaaga accgcgcaaa acaataaaaa aggaatattg
   136981 caaacctaca atatcaatta gttctatgct aattgcctta ttatgtatta gttagctaat
   137041 atactcagaa tgctgatatt gcagcaactg gacagccttt aaatctctta ccatctccaa
   137101 tcgcgcaagt tccacaacct gtacatttat cttgatctat cacggcttta ccctttttta
   137161 tggaaattgc tccaacaggg catattcgaa ggcataattt acatccaata caaatatctt
   137221 tatcgacctg ataaacaatt ctattggcat tagtcaattc ttgggaactt tcttcggaat
   137281 tggaaattgc attttcaggt aatgcatctg taatagatgt ttcatttttg ggtttatcat
   137341 tttttggttc aattgttgct gatttatcta ttgctatttt atcagatatt atagcaggat
   137401 tttttatcaa ttcagaatct tgtattttta tgttatctgc ttcggcttgt ttgggtgcag
   137461 agtcatcttt tacaatttta tcgcaaaaag tgttttggtc atcaacatat ttatgtggtg
   137521 ttaaagcatt gtagaacaaa aagaagagta taaaacttaa aaccaataag acaaatacct
   137581 gtagaacagt tttcatttat ttatcctttt tccatacgag agtatcaggt ttggggcatt
   137641 tttttgaaca gcgaaaacag agaatacaat ccagattatt aacatattct tcttcagtaa
   137701 gatcaatatt catcgggcag tttaatgaac aaaatccgca atcattacag cgttccaaat
   137761 tacgatggat tttaaaacgc ggaaaaccca ctacctttcc caagtattga aataaattca
   137821 atagagcagc ataagggcaa agataacggc accaaaaacg ctcaataaat attcctacga
   137881 taattatcag caaagctaaa attaaaccca aaacagcaat ataagggagc atagagattg
   137941 cataaatagg gcagaagcga atgtagatat agctaatgcc ggcaatggct aaaattgcag
   138001 ttacagcaag gacaccatat tttacaaggc tgaaacgcct ttctaaataa tggggaacct
   138061 gttttttctt tttacttttt ttgttgaaga gagcaaaaat atattcttga atcgttccca
   138121 acgggcaaat aaaagagcaa aaaaggcgtc cgaaaaatat agctaaaacc aatattagaa
   138181 aactgaaggc aatagttaaa ccaaatgcag aactatttaa actacataga aaatttttac
   138241 taaagccaaa acacacagct gagtaagggc aaagattatg aatagtataa ggtaatccac
   138301 aaacaattat gccagctaaa aaaagcatag atattagcaa aaagaggagc tgaacaataa
   138361 atctgagagt atgaaccgga ttttttatgc gcataacagt agttccttta taatcatcgt
   138421 gaaaatatat cctctacact aaaaagaatt attccaacta tccattaatt tgtacctaat
   138481 tctttactat atatagctca ataacttccc ataacattcc catatcacga tatgggaacg
   138541 ataagggaat gatatgggaa ggttacaggc tagctgcaag gatatagtaa ttctgtaaca
   138601 ttttcctatt ttatgggata gtatccatag gagggggaga agttaaatct gatataaaag
   138661 agctaacatc ataagttagt ttaggttgaa cttcggttag cagctgttta attgtgtcgt
   138721 attgtctata ttcctgagca taacccaatg ccagatgtgc gaaatactct tggtcaggga
   138781 aaagcagaaa accctttttt aagtaaggaa gtactttatc gggatactta tctaccgtat
   138841 aagcaagaat atagctcaga tatattttcc acccttcagg atggggggca atagtcttgt
   138901 tgataaattc gtccagtttg ttccattggc cggtcttact gtagtaattt gtgtaaaatt
   138961 cagtcagctt aatttcctcg tggacgaatc ttctggcttt gttaatatat tctaatgcct
   139021 tgggataatc tccgagatca acaaaataat tggcagcacg cacaaagcca gaaccataat
   139081 tcattattaa acggttcata ttatcgtcct tatacacttt cttattgtct atagaacggt
   139141 aattgtaaac cacatcaata ttatgtaaaa gtcgttgtgg atctatcatt gaatcgcctg
   139201 ggttggaaac aactcgggag accatacctt cattacgaac ataatcctca aagtttacaa
   139261 aactctcaca ggtaactgca aaataaattg gacgtttacc ataattatct ttcacaattt
   139321 gcataaccgc taaatcggag atgcgataga aaggttcatc ctttctccat tcagctgttg
   139381 gcggaatatt cattgtaaat cccatagcag gattttctgg aggtcctgga ataacaagag
   139441 gttcttccac tctacgaatg ctgaggtcgt ctaattgttc gtccggaata ttaaataaaa
   139501 tcccttcttt gtctcttagc tgtctaatat accaggcagt atttagtagt gagagattgg
   139561 caacggaaac atctttacgg attcctttca ggcatttgtt tttatattcc atcgctttag
   139621 caactgcttc ttggctttcc gcatttggat aaacatctct ggcagagtaa atattctctt
   139681 tagaataggg atcttttact gcttgtgcat accagagagg aaaagtatca ttatcaccat
   139741 tagtaaatat gatggcattt tcttccagcg aattaaggaa atttagtccg taatctaaag
   139801 cgataaattc ctgagatcta tcatgaactc tgtattgtgt aataaaatta cttagtggta
   139861 ttaagcacat aagaattaac acagcaatgc gaacaggttt acttttccat agggttacta
   139921 aggttaaggc tccaattccc aaccagatag cccacatatt ataggcaacc acaaaaaagt
   139981 aatcgcggtc acgcacttcg gcattggaaa gattcattac aaaaaccata acaatagtag
   140041 taagcagaat cacggataag aaataccaaa agctgtgttt attttttcga aattgtaaca
   140101 ttgctcctgc tacgcctaag aaagcaataa atatattggc aacaggtttc agcatattgg
   140161 aagtgacccc tatgaattta gtaaaaaatg gttcattcaa ccattgcata ctaaaatatc
   140221 ttaaaaaatg ataattaagc tggtcagtaa agaaattacc tctacgttga acaaaacttg
   140281 tattgccata ttgctttctt agaacatatt cttggaacat tttccagtta cttgggttgc
   140341 cttcgttaat aaaaggtctc tctgcagctc gaaccattaa atatatatgc gatgaaagtc
   140401 caaccagtac taaggcaatt cccaataacc aaattctgcg gtctaaaacc ttttttaact
   140461 cgataaagac aattatgaag gctacaagag cgaaacccat tttgtctaaa tcatcaattt
   140521 gcatctgttt tccgataacg ccgaaaaaga aataaccaat tagtagaaaa attgagtaga
   140581 caattaattt gggccagaaa ctgccacttt taatcccttg tttcaataga gggtaacaga
   140641 caataaataa cattgctgga gctatttgca aggcagtttg atgaacacaa aaacccagga
   140701 aaaatagata ggcaatcaat agaagaaagt tctgatgaga aaaatcccgg ctcttttgta
   140761 cccagattaa agtaagccaa aaaatcaggt ttacaaagaa aactaaaccc gaatacactt
   140821 ctgcttcaat agcattcatc caaaaagtga aagaaaaagc tgtgtaaaaa gctgctatca
   140881 aaccagcaaa aattgcttcc caagatttaa ctttcatcat actagttagc tgaatggtta
   140941 taagatatgt aaacattaca gctaaagcac taaaaagtcc ggaaataaac gcagcaatca
   141001 ttccatgagg aacagcccaa ccaaaaatgg caactatcgc tcttcctaaa agaatgtaaa
   141061 aaggatttcc tgggggatga ggaacaccta aaatactgat gcaggttgcg tattctccac
   141121 tatcccaaaa actcatagtt cgtgcttgag tacacatata aactattaaa gtaataagaa
   141181 atactccaaa agcaatccaa cgattccatt ttaccggaac aatgtttgta ggacgaagtg
   141241 attcatcgtc aattataaca cgggcagcag gtttttgata ttccttagtt gttttttgag
   141301 gttctttcgc tttttgtaat tgcgcttttt tctttatttc ttttacatcc actgtttttt
   141361 ctcctttaat ctttaagtcc tgctttggca tagctggcta ttatttgttt ttgtgccata
   141421 aagaatagta tcaagatagg gacgatgctg aaagttgaag cggccatcaa taaagtagtt
   141481 tgagttgatg cttcctgatt aaaataacta agccctactt gtaaaactct caattctggt
   141541 ctattagtca taactaatgg ccacataaaa ctattccagc ttccaattag cgaaaaaatt
   141601 gatgcagtag cgataactga tttggaaaga gggagcataa ttctccagag cataccaaaa
   141661 gtactacaac catcaattgc agctgcatca aaaagttctt gaggcaggga tttgaaatgc
   141721 tgacggaaaa gaaagatagt aaaaatattc acacaccagg gtacaattag tgcctgataa
   141781 gtatccagcc aacttatttt atccaataag acataagaag atacaagata aattggctcc
   141841 ggaaccatca tcatacttaa aaatagatag aaaaagaaat cccttccgaa aaattccatc
   141901 ctggcaaaag cataagctgc cagaggagaa gttataagta cacccaataa ggtgaaaagg
   141961 cttaccagaa tagtattttt aaagtagatg gcaaaaggaa ctttgttaaa tgctttcaca
   142021 tagttctgcc agtggaaata aggtacttta gttatctttt taacctggga gctggggact
   142081 tctttatatt cctcaattat cttatcttct tcatttagaa tatgaataat gctattttca
   142141 cctttttcct gcactaataa gattctctgt tcttgtgttc cattatccca aatataatat
   142201 gtaaccctag gaataaaaat actctcctgt ttgttaaatt ccgattgagt catcaaagaa
   142261 gtggaaagca tccacaaaaa aggaattatc atagccaatc cacaaataat caggacagca
   142321 taaataatgg tcattcgtaa tttttcaccc attttcaact tccttaatag ttaacatgtt
   142381 tttccaaacg cttttgatat atggttaaag caagaatgat aataaataaa gctagtgcta
   142441 cggcacttgc ataacccatt tctaaagtat caaaaccctt ttcataaata taatatacaa
   142501 ttagcttggt agtatttaag ggtccaccct tatccgtcat taaatatacc tgcgaaaagg
   142561 tttgaaaagt gacaatagta gtcattatca acacataaaa agtagtaggt gaaactaaag
   142621 ggagagtaat tttgaagaat tgttttattt tactggctcc atctatatca gctgcttcat
   142681 aatagacctt ggaaatattt tgtaaaccag caagataaat aattgtattg tagcccagtc
   142741 ctttccaaac agtcataata attatggcaa aaagggcttg agatggaccc ccaatccaac
   142801 catgaagatt aatacctaat ccagaaaaca gcatttcaaa aatccctctg gattccgata
   142861 accatttttg aggggaaata ccaatgtatc caatcaaagt attaatcaag ccagtttgtt
   142921 cattgaacat taatttccaa acaatggata ccgcaaccag tgaagttaca taaggcataa
   142981 aatagacagt tctgaaaaga cccttcaggg cttttatgtt atttaaaaga acagcaaaaa
   143041 gcaaagatag tataatactt gcaggaacaa caataataac cagccaaata gtatttccca
   143101 tagatcgcca gaaaacttca tcttttaaca tagttgcata attcattaat ccaataaatt
   143161 tgccagttcc ttttatagac caatcgaaaa agctgataat aaagctcata acgattggta
   143221 tcaaacgaaa agccattagc aaaaataatg cagggaaaat gtagagatag ggtgaaattt
   143281 tccatttcgc tttcatgctg tagtttcctt tgtttctttc tgcagattat tacgggtgcg
   143341 aaattgttta tagtcaattc ccagtttggc acatttatta tataaatttt ctctggtgat
   143401 gccactaatt tttgaagctt tactgaaatt gccattacat tgggaaagca attgatttaa
   143461 ataacgacgc tcaaaattct gcttggcaga taaccaatca ccatattctt cttgctggaa
   143521 agcatgcgaa atcatatttg ttgaattaac aatattttcc ggtagatctt caggtaaaat
   143581 aacattatgc tcggctaaaa taacagcatg ctcaataaca tttttcagct ctcgtatgtt
   143641 tcccatccag gaataattca ataaaataga cattacttca ggagagagtt ttagctcccc
   143701 cttattatat ttattacaaa attcattagt gaaggcataa gcgagcactt taatatcgtc
   143761 tctgcgttct cttaaaggag gaatatggat tttaaaaaca tttaagcgat aatataaatc
   143821 ttctctaaag gtgccattgg caacgcattc cccaatatct ttgtttgtag ctgtaataat
   143881 tcttacatct gtatgaacat cttcttcacc accaaccata cggaaaaaac ctgtttccag
   143941 aaccttcagt aatttaacct gaaaggaaag tgatgtctct gttatttcat ccagaaaaag
   144001 agttccgcca ttagcttcct gaaaatatcc aattttatcg cgataagcat ctgtaaaagc
   144061 acctcgctta tgaccaaaaa gtaaactttc caatagagtt tcggttaaac tgccacagtt
   144121 cactgttacg aatttttggt gtttacgcaa gctgttacga tgaattaaat tggcgaaaac
   144181 actttttcct actcccgttt ctcctgttat taaagtggaa gtatccagtt tggcaatttt
   144241 tctcactgtt tgcataattg attgaattgc aggagaatta cctattatca aatctgtttc
   144301 aatatcagta tcaattaaag cgctaagccg tttattttca gctattagcc attgagtttg
   144361 tagtattttt tctacttgga tattgataat atcaagatca cgaaaaggtt ttaccagata
   144421 ttcattggct cctaatttca tagcagcaac agctgtttca attgatgctt gaccactgat
   144481 agcaataaca gctgtgtgag gataactttg agtaacatat tgaatcagat caagtccatt
   144541 ttctcctgga aggataaggt ctatgatagc aatatgataa ccgtaattat ccagaagctc
   144601 tttccctgct tttaaacttg tagcggtatc caccttgtat ttattcttga gctgaagctc
   144661 aaaaagctcc aaggtctcaa cgctatcgtc aacaattaga attctgggtt tacttgtttc
   144721 attcatatca attttaccat agtaaccagt ttggcatttt catatttcaa gtcaacctta
   144781 atatcttgat tgaaactgaa aaattcggga ggaaggttca aatctgatat atcagcaaga
   144841 taattgcttc cattaataaa gatttttagc ccctgctgat ttacaaatat ttgcattgat
   144901 atttggcctt caggtatatg ttcagtgata aacatcaaaa aagattcaaa ccattcactt
   144961 aaagcgatag gtgtaatttt ccctaagatc tctttttcgt aataatgcgg agtgaagata
   145021 aagcggtgtt tgatctccag gtagttatgc aaaaagttaa gttctgcatc tagccaggta
   145081 ttaagaggtg tttttgaatt tccaccacca cgaatttgaa tcgcctccca agtttcttga
   145141 aggaggtctt ctaattttat accggcctta taaatctgtt ctgcttcatc acaattaact
   145201 tgctgtttga ggcgatgaac ataacctaaa atcaggttta agggagttcg gatattatga
   145261 attagtcctt caagaatagc gggaggcaaa tctttcatct tatttatgaa tcctttggta
   145321 tgtagcaagc cctttggcaa agatactcat tgcttttttc agatcttctt ggcaaaggat
   145381 atatgctaag cgaagttcat ttttcccaag cccttcagtt gcataaaaac cgtctccagg
   145441 agctgccata actgtttcat tatcaatttg aaattcctta agcatccaaa tgatgaaatc
   145501 ctcagaattt tcaatgggta gctttacatt aatataaaaa gctccttcag gtttccggca
   145561 aacaataccg gggattttat ccagttcact cataaccaga tctcgacgag attgatattc
   145621 agttcttatt tgttcaaaat agctatcagg taaattaata cctgcattgg cagctaattg
   145681 atcaaccgta ggaggacaaa gtcgtgcttg ggcgaatttt aaaacggaag ccattaattc
   145741 cttatttcta ctaacaatac aaccaattct tgcaccacag gcactatagc gtttagaaac
   145801 actatccact aatatggcat tttcttcaaa atctggaaca tctaaaacac ttgtatgagt
   145861 taaaccatca taaataaatt cacgatagac ctcgtcagat atgaaaaaga gacctctgcg
   145921 ttttgctaat tcggcaatgc ggaatatttc atcgttacta tatacagttc ctgtaggatt
   145981 tccaggattg caaagcataa tggctctagt tttatttgtt attttggctt caatagtaga
   146041 attatcagga agagcaaaac catcttctgc gtaagtagtg atgggaacca aatttattcc
   146101 agccattgta gcaaaaccgt tataattggt ataaaatggc tcgggaacca ttatttcatc
   146161 tccaacttca caaacaacca tcatcgcaaa agtaattgct tcacttcctg cagttgtaac
   146221 aataatatca tctggcttaa cttcaattcc acaacgctta taatatttaa ctaagccctc
   146281 tctataaaga tcaagacctt gcgatggacc gtaagctaag atcttatctg aaaattcgtg
   146341 atacgccttt agcatttctg gaggagtatg tagatcaggt tgaccaatat ttaagtgata
   146401 aactttaatt ccttttgctt tagtggcatt ggctatggga ttgagttttc gaataggaga
   146461 tgcttgtatc tcccttgctc ttttggatag ttccattttt tgcatcctta ttattatatt
   146521 tttcttctga ttcctttatt taaaagagca gaaaaaagac aaggaaaatt ttgttacggg
   146581 agaggattta tcaagctatt cttttgtcac aaggtgggtt gaagtcctcg ttaataacag
   146641 ctataagttc ataacctact gtataagata aaggtataag aagttttgca gagaaacgat
   146701 cagatctcgt ttttcctgtt atataacaaa gttatgggcg aaattatatg cttcgggagt
   146761 atctatatcc atccactgat ttgctttatt tagcatcacc actttaatcc tttttaaaga
   146821 gatcaattca gttatcgcgt tactgataga ttcattacct ttttgcactg atttttctac
   146881 tgcagtaaag aaatcaggtt ccaaacggaa tatgccacaa tcgagagcat tataattatc
   146941 aagttctttt ccaatagcga ttatataatc atcttcggta agtacttttg tagcatcgtc
   147001 tacatcaaag ttttcggaga taaagggatc cgttagcagt aaattaattc tttcaggaac
   147061 ttccactatt tttttaaggg cttccatttt taccaggtga tcagacatcg aaagaagaaa
   147121 gggttcgtta ttaatatagt cctttgcttt ataaactgaa agagcattac cgcgttccca
   147181 ttcaaggttt tccactaaag taacaggtaa ggtaggtgga tcattttgca aatagttagc
   147241 aatataatct ttcttaaaac caaccactat aatcagttct gaaataccag ccatttgcag
   147301 ttgttgaata atagtggata gaatagttcc ttttttatag ggtagaaggg ttttaggaat
   147361 ttgttttgtg gttttccaaa gccgacttcc aaatccagca gcgataatta cacctttcaa
   147421 aatttaagct ccattttcgt gaccaccttc atagtaaagt tgttccattt tttctacaaa
   147481 gcggatactg gcttttccat caagtccata gaaaaagcgt tgacggtatt catctgcttt
   147541 agttttcatt tctttagtag gattcagggc tttttcaata gcagtttgca gttgttttcc
   147601 gttttggata tgaggaaaag ccccttttaa aaattctctg ttatcaattt ccaatagggg
   147661 ttggccgtca gaatggttta atctatcaca atcaagatca tatacaatcc cgaatttatt
   147721 tagagcaatg aaatcgaata ctgtactgga agcttcactt aaaacagtat cagcagctgc
   147781 ataataaggc acaatattaa attctgtaaa aggcaaaagc acagaatgag gatatttttt
   147841 tacccttttt tcaaaaatac gatgttgttt atgcggggca tattttccca tccaactata
   147901 gggatgtagt tttattatca gattatagtt tctggtttgg tcaaagatat catcttttat
   147961 ttcgtacata caggtgggct tataagtagg agcaaacaat actgtttttt tagcaggatc
   148021 taatccccat cgttctaata tttcttctct tttgaattta ccctgaaaat aaaagtctag
   148081 cttaggtaag ccaatcagat ggacttcact tttttctaaa tatgggcatt ttaacaggga
   148141 ctcaacccga tgtttacctt ctacaaatat ttgatactta actcccggag tgcgtgccaa
   148201 attgcgatag agaatatttt ttatcccggt tccatgattc aaaaaaatca atagagtttt
   148261 gccatagtcc tctgcattcc gaagggtatc gccagaaata acaatatcga aacctttagt
   148321 ttcattggta aaacgataac cgagattttt ccagtcctca attattctat tgcgataagg
   148381 aaattcaaag atgaaatact tgattttttc catatccaag gagaaccaga tatcgtattt
   148441 ttcatcgttt agcaacaggt ctataatagg atcaaaagct gctttatggt aagcataacc
   148501 aattctaaac aggagtttta tcttcttcat ttaccgtatt ttctttggta tttacaacta
   148561 ttaaaataag tatggaggca agctccatac tatatattta catttcgtta taatactcca
   148621 aaccaagatg agtgataaga ttttccttca tcatatatcg caaagtattt ttcagcttca
   148681 tttgttgaat gaaaagatcg ttttcaggat agagtccagg agccgtcata ggtgatttcc
   148741 agaagaaaga tagccattct tgtactccag acatttcaca acgctgtgcc aaatcaataa
   148801 ataaaatcaa gtctaataaa acaggagcag ctaaaatgct atctctgcac aggaaattta
   148861 tctttatttg cattggataa ccaagccagc caaagatatc tatattatcc caactttctt
   148921 tattatcacc acgagggggg tagtaattaa tccgcacctt atgataaagg tctttataaa
   148981 gttgaggata tttttcaggt tccagaatcg tctccagtac tgaaagtttg ctaacttctt
   149041 tggttttaaa gctgtcagga tcatctaaaa cttcaccatc tctattacct aaaatattag
   149101 ttgagaacca accctggata ccaatcattc ttgctttcag accgggagcg atgatagttt
   149161 tcattagggt ttggcctgtc ttaaaatctt taccaccaac gggtactttt ttctcttctg
   149221 ctagtttcct taatgccgga atttccaaat ttaaattcgg agcaccattt aaaaaaggaa
   149281 ttcccatttg taaagcagca taggaataaa tcattgaagg agcaattgct ggatggttat
   149341 ctttcaaacc ttgttcaaaa ctttcaatgc tattatgaat aggatcagct tctaaataga
   149401 cttctgtaga tgcagcccaa gccataacta gacggctgca atttttctct tctttaaagc
   149461 gtttgatatc ttccatcaac tgatgagcta agttcatttt attagtgccg cttttaacat
   149521 aagtgccatg taatttcttc acatatttat tgtcaaagac agctttatgg ggcttcagtg
   149581 aacgcaattc tggttcaatt ttatccaaca gttctttatc caaaactcct gcttttaaag
   149641 atgcttcata ggcatcgctt tcaaaaatat cccaagctag aaattcaata tcctgtaaat
   149701 ctgcaagcgg gacaaaatcc tttattaacg gggttctatt ttctgtcctt ttccctagac
   149761 ggatggttga catttgagaa acggaaccgt agggttcgga aatgcctttt ttaattgcct
   149821 gaacaccagc cacaaaagtt gtgctaactg cacctaaacc aactaaaagg acaccaagtt
   149881 tgccctcggc gggttttatt tcattacctc taagcattgt ttttctcctt actaatattt
   149941 gcatattttg agtcactaat aattaaattg tactgatgtc aagtacaatc cgcaatggga
   150001 aaatttgcat atataattgg gagatctttt tcagctaaaa cagtatgtaa ccctctatac
   150061 aataaaatga aaataacttg agtaatataa cagttacgag ggaaaatacg ataatagtct
   150121 attatgggaa gataagaaat tattcggaat tgtaaaaaag aggtttacaa cagacattgc
   150181 cgatattcat tgccaaagtg aggaattcag cttatattat atataagtat tttgttgaaa
   150241 atgagtataa aaggctgggg aagagttttg gagcatgaga ctaattttaa ggacagctta
   150301 gaaaatgtat tagtattcat ctgttagata gagaagaagg actttaatta atataaacac
   150361 agataaaaac tagtgaggac accaaagatg attaaggact taaacgaagt agattttgac
   150421 aaggcaattg ccggagacaa agttataatt gacttttggg caccctggtg tggtccctgt
   150481 aagattttct cgcccacttt agatgagatt gataaagagg ttgctgacct tacaatctat
   150541 aaagtaaatg tggacaacta tcctgcctta gctggtagac aaaatgttca cagtatacct
   150601 actctgttgt tttatcgtag aggtaaatta gtagatagct caataggagt agtatcaaaa
   150661 agtgtgattt tgcaaaaact ggaacgaatg aacgattaaa tcaagatatt acttgtcagt
   150721 tcagggcact tgaaaataat ggtgtcttga gaaaattctt actcaagagg aatattaagt
   150781 gcccaatcat aagctaaaaa atagaataga atatgtaagt ttcagaatag cacttgctgg
   150841 attgaaactt ttacctcctg aagcagcaaa agctgtatta aaaggcttgt tttggtttgg
   150901 gggttgggtt ataggtattc gcaaaaatgt tgcgttacaa caacttagta agatttttcc
   150961 ggataaaaac agcaaagaga aagctgctat tatcaaaaaa ctatatcgta atatggcatt
   151021 atccatttat gagtgctacc taatggatga tgaaacacta tataaaaata tccgaataga
   151081 aggtagagag tttatagaag aagcattatc cctgaaaaga ggagcaatat ttgccacagc
   151141 tcattatgga aattgggaag cggcaaggat tttacctaaa gcaggaattc caataagtgg
   151201 tattgctaaa ccccaaagaa atgttctatt tgataaatat actaatacaa tacgcgaacg
   151261 atgtgggatg cggataatca atatgaaacg tggacttagg gatatagtcc acgaatttag
   151321 agagaatcgt attgtagcaa ttcttatcga tcaaaatgca ggtggatcag gtttgttaat
   151381 gaatttttta ggttttcccg cttctcactg gaaaggagtt gctaaactct ctttgcgcta
   151441 tagagttcct atcgttccct gttttgctcg caggtcggaa gatgatagaa tagtttttga
   151501 attctttcct gttatcttgc ataatgattg ggatgataag gaagaaaact ataaaagtgt
   151561 tctagaagaa gtaaataaaa ttcttgaaca tcaaatcaga gaacatccag aacaatggtt
   151621 ttgggtacat ttaagatgga aacattccta cgatatgctt gcctaagggt tgttttcgca
   151681 tcctgaaata gctatggagc agttatatgt ttatagatca tcgatatgaa gttttagaac
   151741 atctggggac aggtgcatgg gctaatgtat ataaagttcg cgatattcgc agcggaaaac
   151801 tttattcttt aaagcttttc caatatgtct cttcagatga tttatactcg cgtttcagcg
   151861 ctgaagaaat gcatcatata actcgtcttg aacatcccaa tcttagtcat actgtcgatt
   151921 ttgggcatgt tgcagatcat atttattata tcagtgaata ttttgagggt aaaccgctta
   151981 ctggtgtcaa attcactaaa gctaaattag gccaaattta tgatattgct attcagatat
   152041 gttatgcttt gcatgcttta cacactcaaa atattctgca taaggacttg aaactggaga
   152101 atgtattgta taggacggaa gggaatacaa ttattcttaa gctgattgat tacggctttt
   152161 ccaaagttga tactactaaa gattcacagg cagtttcggg aacattacct tatcttgctc
   152221 ctgaaattta tttgggaaaa cccgctagca aggctagcga tttttatgcc ttgggagtaa
   152281 ttttatataa acttactacc ggtagttttc cttttagtgt ggatcaaata aacgcactta
   152341 ttactggtgg taaccaatat tttattccca gttttcccag tgaactgaat aaagatattc
   152401 ctctttctct ggaaaagttt atattacgtt tactagatca aaatcctgat aaccgctttg
   152461 ctagttgcga agagattgtg cattacataa atcggactca atccttccaa tatcctttct
   152521 cggtgtcctg gtctatgata aacaccttac attttaatag ttatcttgtt cgggataaat
   152581 attcacatca gattttagat tacattcctg cagtagaaaa aggaaatggg aaaattatat
   152641 cagtaactgg aggagatgga ttaggcaaag atagtattat ctccttattc cgctatcatt
   152701 tattaagcgg taagtacttt atttttgatt acacctgttc caaaagcgat catgaggcat
   152761 tttttgcttt aataaaggaa tttgttcaat ctctaagtcc cgaggaactt caggaataca
   152821 gtgggctgaa aacgatttct gaaaaattcc gttgctattt atttggttca gaacaggaag
   152881 ccaaaactgt aagccagaca agtagtgaat tgaagcttga ttttgaatct gtaaaaagtt
   152941 tgcttataga cctttcaatt aataaaccta ttatttttat cattcgcaac ttccagtatg
   153001 tgcatcataa tacgattgat tttattaatt ttatgtcctc ttatattgtt aaacaccgta
   153061 taatgattgt tattacctgt aacgattata ataaaataaa tcagatagac catacagtgc
   153121 taatgaacat ccctactcta aatttggatg aaagccggaa ctatatcaat cgtttgctat
   153181 ccggtttttc ctcttctgac aatagagata gtgttcctga agaattaatt aatgaaatat
   153241 atagacgctc tgcagggaat ccttacttca ttgtggagat attgattgat cttatgcaaa
   153301 gaagaaagct atattacgat aaaatgttac actatcctgc cgatctttct gattatcaat
   153361 tatcatctcg tttattgcaa tcaattcact cgcgcttgag tcacctaaaa gatgtaaatt
   153421 atttacactt gcagaaacta agcgtagtgc aaactccttt aacccgagaa ttgataatct
   153481 atattttaaa aataaaggat tctgaacttt attctctatt gaatgatggt atataccacg
   153541 aaatattaaa gaaacagggc aaaaactatt attttacttt tcaggaagct aaacagcgtt
   153601 tatttaatga atgcagggat aaaacacata aattagtttc taaacgcgtt cttaagtttt
   153661 atgcggatga gattattaca gattttgaaa cttgccgtgg tctgattgcc aatgcttttc
   153721 tggccaacga tctgatttgc gctcgcttat attatcttag atactataat atgctcaatc
   153781 aggaaaatga acaagaacaa gctttttttg caattttaaa tgttttaaat ctggatttta
   153841 atcctgaatt gtctatctct ctaaaagact taatttctga tgttaaaaaa ttgcaggaaa
   153901 aaatggaatt cacgggattc tatgaacgag ctaagttcat aattcaggaa ttgatgaatt
   153961 tacctgattt tttcgagaaa tactatcttt tggggaccat aaatattttg gcagaaaata
   154021 tccagaaagc cctggaatac tttagcacag gtgagaggaa tattattaca ggaagacagc
   154081 aattattact ctggcttgcc tatgctcagg tctttgctaa aattgatccc tctaaaatga
   154141 tattatatgt cgataaagcg ctgaagcaag atatgacctt ggaattgcat attgcttttg
   154201 ttgaccgttt ggcagtctat cattcgttaa tgaaaaattg ggatatggcg atcaaaacta
   154261 tagctgattt tttggaaaaa ttacctcctg aacacaatac tgatgttatg tatcggttga
   154321 ttgtgataca caatgattta ggcgtttttt acagtaatca gaaaaatatt gatgaagcgg
   154381 atgaacacct ctttactgct ttaaatattt ccaggcgata taacataaat cgctatttgg
   154441 gccttattta taataatata tcagatttat atctgaaaca agggcttacg gttatttctg
   154501 aacactattc ggaattagct tataaatatt ctgatgaact caattttgct atgtctaaag
   154561 ctctgtctct tttaaatcaa ggtgaagcaa aaataaagat gggagatttt gttaccgctg
   154621 aacaaagact tttagaatgc gaggcattag ttctttctaa ggaaagcaca aactacttgc
   154681 aatcggttaa aagaaaccta gcattagcta aaagtaagat aaagggtttt ggccattatt
   154741 tcagtttcat tatggataat gaaccagaac tgatcagttg tcggataaaa gaattgaatc
   154801 ctttagtgaa gacctatttt tattatctgc atgaaattgg aaatgcgaag aaattaaggc
   154861 gtttgttatc gaaaaatgta catataaact ataaacatat acatgaagag gaattctatc
   154921 ataatgcttt ggccctagct tctatggcag aaaaggactt tgagggtgct ttaaatgaac
   154981 tgaaaatcgc tttgcgtcat gccggagaaa ttaataacaa ttatgcgatt gcggtattct
   155041 atgtattaca aatccgctgt tactatggtt taaaagatgt agaacgagcc agagaactaa
   155101 ctgaattagc tcttcccaaa ataaaggaaa accattatcg ctattgggaa tgcgaattgg
   155161 ctgttttggc gcttaaaatt gatcttttag ttcccgatat acctttaaga tcaatattga
   155221 gaaaagtgaa tgaatacctt gatgcttggt ctaactatga atactatcaa ttgaatgtag
   155281 agctctacca agtaaagata cagatattta tagaattgaa acaggaagaa ctggcagaag
   155341 ctgaatatgc aaactacaaa aagtatctgg agaatatcac agaaaatatc agagctgatg
   155401 accgacagaa ttatctgagt ataaatctct attatctgaa taatctgcgt aagtttaatc
   155461 tgttacccat tgcttccaga acgaaagacc tgatgaaaaa atggaacgag atgctattta
   155521 atattactaa tatcaacagt attgatagga tcaagtttct tatagagaaa ggcctgattc
   155581 aagtattctc gccttggcag ttttatctga tgaaatattc ttccaaagta tcttcctata
   155641 cctgtttcca gagctataat accggaaaag aaaacacttt caatacagaa cttttaccgt
   155701 atatagaaag agcatttaaa atagacaacc tcgtaatcct tgaacaaaat aatctgcatt
   155761 ttatggttgt cccttttcag attggttcaa aaagaattgg ttttctgctg ctatcggata
   155821 agagtgaaat gccttttacg aagcaagaaa tgtctcttat gcgagatatg aaacaacatt
   155881 tggctgctct attagtaaga atagaagatt ttagccagat tactttacgc atacaaaaga
   155941 tgaaccagct gatggctata acacacgaat tgatgcggat tgtaaatata aacgaattgg
   156001 aaagagccat agtatctgcc tgcattgatt ttactaatag tacaagaggt tttctgatta
   156061 agcgtgatag tgatggagcg ataatatata ccgtgcagct caatgcagca aaacaaattc
   156121 tgcccacggt atctggagtt agtaaaactg ttttaagttt gtctcaaaac actcaggaaa
   156181 ttgtctccac tttcaatgcc gtggaagata atcgttttaa gagtgcgata agtgttcagg
   156241 attatgtttt gcacacgatc ttctgtgctc ccataattgt agacaacaat gtgtatggct
   156301 atatctatct ggataacttg aatgataata accgcgaaat gtatctgaat acagaaataa
   156361 taaagctgct attggaacaa attacaattg ctctgaaaaa tgctatgctc tatgaaaact
   156421 tactgcaaaa gaataacgag ctaaatacat ttgaaacgct gaaagacgaa tttatggcaa
   156481 tagtatctca tgaattaaat acaccgctaa caactttgca gggatatgtt acgagattaa
   156541 aaagaaatct ttttgctgat gaagaagaaa agaaagatat tatcaacaaa attgagaatt
   156601 cagtgaagaa aatcattctc actatcaacg atattactac tatgaatacc tataatttag
   156661 ccaagtcgtt gcccttttcc actctatctg tgcaggaaat attggaaatt atctatcagg
   156721 aagtccaaat tctttctcgt aaacgtaaga tgttcataaa attggaaatt gaaaaagact
   156781 tacctccgat taaagcaaat tgggaagcgt ttcatttaat gatttataat ctggttctaa
   156841 atgccattcg ttttaccaac gatttcggga ctgtgattat tggagctcgt aaatctgctt
   156901 ttcaaagcga aaagattgac ggtaaagagt cattagttat ttatgtacaa gataatggaa
   156961 tcggaattcc ggaataccaa ttgaagaatg tattcagaaa attctatgaa ctgaacgaaa
   157021 tctatgctca taaatctgga actatagaat atcgtagtag cggtttgggt cttggactgg
   157081 ctacatccaa acgcattgct gaattgcatt ctggtaaaat ctggataaaa agtaaggaaa
   157141 atgaaggaac aactgtattt gttacaatac caataaaaca aagtaaatag gtgaaataaa
   157201 caacttatga aacgatatat tataatgatg ttatgccttg tcttgatgtg tatttgtttt
   157261 gctgaatctg taggaaaaaa gagcagccct tctttgttgg cagtaagtaa tagcgaaaca
   157321 tacgcatatc gttatattgt agaactttac gatgcaggtg atgcttacaa tctgagtaaa
   157381 gaaattactc tgtttaaagc tacctatcca gaatcggtat atttacccta tataaggttg
   157441 attgaaggta atcttgctat ggaaagtgga aattacacta aagcgataga aatatatgaa
   157501 ctcttactga atatcgacct tgttcaaaat gtgcgtaatg atattttgtt gaattatgct
   157561 tattgcctga gtgagttaaa acaatatgat agagcattca atatattaca gcgtttggat
   157621 agtgaaacag taaatccagt gtatatcgaa ttatcaaata acttgagagg agatatatat
   157681 tttcatttag ggcagttcta ttctgccgaa aaggcatata aaagagcaat ggtagcattt
   157741 cctgataatg agggtatccg tttttccttg ttttctactt atttggcttt agataaagat
   157801 gatgatgcct tggaactgct gaaaacaata actcctgaaa gtgattatta ttataaatat
   157861 ataggtagct ggctgggata tctattagat aatgaacttt atgaagactt tgatagattt
   157921 gttgaagagc agaaactaga ggatatcact tctaatctgg atattataga tatcagaatt
   157981 cgcagagctt tgcacatagg tgattttgaa aaaacatctc aacttctgca aaaaattaca
   158041 attgaatctc ctcagtttca atattatcag gcactggttc tgataaatgc aggtaaagaa
   158101 aagcaagctg attctctatt ggctttgctt gtttcctctg ctagaccaga aatttctgtt
   158161 ccggcttata tggaaagggt aaaactatta ttcaaaaaga atcctaaagc tgcaattgag
   158221 cagctgaaca gctatttgaa acaaactccc agcgacttta tgaaagccga attatattac
   158281 actatgggct ttttctatta taatacaggt aactattctg aggcaatcag acatttagga
   158341 atagctcgcc attatgaaac aagccgcgag ctttgtagta gaatagatat tctgatagca
   158401 gaagcattct atgcatctgg aaataacgat ttggctaaag atacattcaa tcgctatctt
   158461 aatctttatc catatggcag cagaagagat aaggcatatt tctatcttgg ctatatgagc
   158521 tttcaggaaa aggattatac ccaagccaag gttaactttc aaatgcttca agagcaatat
   158581 ccaaattccc cctattgtaa tgatgcaact ttctatttgg cagagatgga tttctattta
   158641 gcaaactaca atctagcatt ggaaaattac ctttatttgt acagtaaaaa agtagataat
   158701 gaagtagttg ctttacgcat tacacaagta tattactatc tgggagatta tgaaaagagt
   158761 gaaagttata tagcaaattt agttcctagc tatgatacct gcatcttaaa agggaatatt
   158821 atgctggtaa aaaaggaata tgagaaagct atagaccaat ttttacaagc agagaaatat
   158881 gctccagata atttaaaaac aatagaagcg aagagctatc gggctttgtg cttatatcag
   158941 atgaaacatt ttaaagaagc atcagctttg tacctacaac tttctgggca aaaagaaagc
   159001 ccagatacat atcttttcct agctgctaaa tcggcttatg ctgctaaaga ttatcatttg
   159061 gctctggaat tatacaattc ttttatagat aaatatccgg aatcaacgca ttttttggaa
   159121 gcgctttcca atattgccaa tacctattat aatatgggta attacgatag agctattaat
   159181 gactggataa atatcttaac caggttcaga aaccaaacaa aatttaccga taaccaactg
   159241 gcaactatac aggatgcctt aattggtttg gaattggctt tacaatatgt aaacaaagaa
   159301 gatcttctag cggaattgct agttttgcca gatacttttt tcagcgaata tatcaaattc
   159361 gaattgaatt atattctggt gaaagtttat gcaaacaaaa aactctgggc agaccttatt
   159421 gccagagccg agaaaatgaa agaggatttt ccccaatata ggcaagaaga tgtggaattg
   159481 ttgatggcta cagctcttat tgaattgaac gaatatacaa aggcagattc attgctatcc
   159541 gaattgtatg ctgaaagtag aaataacgat actttactga aatgggcaga actcgaattt
   159601 attacaggaa attatgaatc agctttggaa aaatacaaac tagcttataa aaacaatcct
   159661 aatagtgaaa tttgggttaa tatgctaaaa tgttcagaag cgaataatta taatgaattt
   159721 gatcatctat ggacactagg aatagacttt ctggaaaatt gccctgaagt aaatgttttc
   159781 cgcattcgcc agctctatta tactgaacat tttgaagaag caaatcagct tgcagaacac
   159841 cttattaatt atagtttatc cacttttgat catgctacag cttttttagt gatggggatg
   159901 attaattaca gaaaaggaga atatcaaacg gcaatttcta ccttaagtaa agtggtaatg
   159961 ctctttccag aacataaaga tatttgtagt aaagcaataa actatatagt tcgttctctt
   160021 cttgattcgg gagcatatac agaagcggaa gtgtttttta ttcaacatga aaacgaattg
   160081 acgataactg acaaaacaga attgacagaa cttttggagg gaatataatg aaacgcttct
   160141 ttttaactct tgcctcaatc cttactcttt cattattgat agctcaaact caggagcttc
   160201 ccgatttaac aattagcggt gagagttcct ttaaacccta tctacataaa cattctttac
   160261 tatttgacca ttcacttgtt atgggggata gcttgcctgc ttttattccc ccagctgttt
   160321 ctactaacga aaatagaagc gaaagctata ctatgcagca tcgatgttat cttcaatttg
   160381 aaggtaatgt agattatgga ttcaatagtt tcatcagtta ttatccaacc agtgaagtgt
   160441 taaatgctct aacctatact ttagatatgt gctctcctgt ttcgaaactg ttatctgttc
   160501 ataataaact cttcttaggg acggaattca gttctaagct tcctctctct tttcggctcc
   160561 aaaatgtttc ctcgcatgct gatgattttg ataatactct tttagatttt actgttagcc
   160621 atcatagacc cattgtttcc ataggagatt tcattttaaa agatatttct gcacaacttg
   160681 gatatagtta tctataccag caaaacctgg gtagttctta taaaggtaac tacttaaatt
   160741 tatatcttgc ctcacgcttg gatgttgatc cagtggatat aaaagcgaaa atccttacca
   160801 gagaagggga tgtaggattc caactatcta caaatgctaa agatgagtat atgaatattt
   160861 ccagattagg ggttagtgta ttagctgatg aatatacttt catcccttct ttagaatttc
   160921 tttatcgttc tcctattcaa aagttcggtg tagtgagtat tgctaatgaa ccgctgttag
   160981 aaagcaatac ttttctttcc catatggaag aaaatccctg gatagctttt gatgatggac
   161041 ataagctaaa gaaaataccg cttaacttca aagtaggttt agaataccta tatcccgaaa
   161101 aaatgaattt cactttagcc tatctaacca tcaagaataa tagtcgttac gaaatagaca
   161161 gccctgtact tatcaatagt atcaattatt ctattcccac tgttcgttat acggatatat
   161221 tctccaatct aactataata gaagcgtttt tcaagctgga tgaagttaat atacatcagg
   161281 gaatggaatt agatctatcc tatttgagcg aagatgatta cagaagagca ccctaccgtc
   161341 ctcttctaaa cttggattct cgcttttctt acaatttttc cagatggttt tttagtttgg
   161401 acatcttgca gaaatacttt actgtaaatc acttgggtaa tgatctacct gaagctgtaa
   161461 ttatcaatat aggtgcagaa tatcgtgaag caaattatgc agtctatttt gaacttgcta
   161521 atctctttaa tcgcaagcaa tatgtttttt cagaattacc tgcacaaggt cgtaattttt
   161581 atcttggttt gaaacacaga ttttaggatt ttattgacaa aataccctat gtataggaat
   161641 aaagaataca atgttaaaat gaaggtaaat aatgaagtta aaggtgctta atctaatacg
   161701 cgaaattctt aatgtcctgt ttggagatta taatgggata caggtattta ttgcacctgt
   161761 aactcttgta tattggatag atagcaatag cttgatcact tctgcaacca gtttgatcag
   161821 ttttcgaatt cattatcttc ctttacttgc tttttttact attctggcgt ttgtcgtttt
   161881 tatgttggta aaaattaaat tactatatcc ttgttccggt agtgagttta ttgatttaac
   161941 aattcaattg aatgtgagcg taatggcttt aattcttatt gcactgatta tatatgcttt
   162001 atcaaatttc ttggcttatt tctatgggat aaaaggaacg gtaaaatctg gattatatct
   162061 gcttttcaaa ctatatacag caatattaat tctttatcat tatctcatta atgtggtttt
   162121 agctcctttt tatagaagac gatacagcca tttgcatgcg cttaagaact ttcttgtttg
   162181 ggcaaggcat aataaatttc tgctttttcg ctatgtttta ttgatagtta tggtgatctt
   162241 ttttgcagtt cgtgtatacc aaatagtcct acaatttctt attataccag caatcaatag
   162301 tattagcgat tttacgggct tcgatttaaa attcaagtta tatccatttt taggagttag
   162361 tgatattttt acaaatatac tggctatatt tggtgctttt acggtctcca atttactctt
   162421 ctttcccata atctgggtat taaaatattt ggtagaccgg tttttgcctt tttccaatct
   162481 aataaggtct aattatgccc aaatcacata agaaaaaact gcgtaaaatt aaacgccgca
   162541 gaaagaacaa taatctaggc gtatttattc ttgtgggatt cttggcttgc tttatcattt
   162601 ttgtgataat tagagagcca atcaatgaac aagttgggaa gaaaacagat actgtagacc
   162661 agaaacaaga tatgccttca agatctaaga aaagaaataa aaaagaaggt aaaatcctta
   162721 cacatgacaa gaaaagcact tctgtatcag ataggaaagc acttggaaca aatcaaatac
   162781 gagaagaaaa aaacacgccg gaagtagcta ttgttaacac aattggcaaa ttgggtatac
   162841 ctaaaagcta ttaccgcaag cgtaatacag aggaatatac tatatataat gttcctattg
   162901 ataaatcgat tatggacctc gtttatgcta atatgatttt caaaggcgaa ttggaaagta
   162961 atggagctaa attcatcaag ggaacagata atcctggtaa acaaacccta ctatttaatt
   163021 tccctaatgt tcctaaacaa tatcaaataa acatatacta tgattctgaa ctatacaaga
   163081 ataaacagac aagacgcaca attacattag tggtggacga ttttggtccc gtcggtggtg
   163141 acctgcttga gggttttctg gctttggata aagaaatctg ttttgctata ttaccagatg
   163201 aaacttacag tgttttaaca atggagaaag caaaagaaca gggacgaact accattatcc
   163261 atattcccat ggaaccaatt ggttacccag aagtaaatcc tggtaaaaat gccattttag
   163321 ttcagtatga agatcgtcaa attgataaag tcctttctca ctttatagag caattacctt
   163381 attgtgaagg agtaaataat catatgggca gtttggctac tacagatgaa gaagttatga
   163441 gtgctgttat ggcaactctg aaaaagcata acaagttctt tttagatagc cgtactacaa
   163501 atatctcaat tgcctatgct atagctcaaa agtatcatat caaatcttat cgtaatgatc
   163561 tatttctcga ttctcctaat atcagtcaat cgactatgga tgcaaaatta aatcaaatta
   163621 ttgaactttc caacaacaac aaaaatgtta tagctatatc ccattgtcat aattttgaaa
   163681 agttgcaata tcttaagcgg ataattaatc gtcttaaagc tgctggcttc actttggttc
   163741 ctatcacaga aattgataaa tacaatgttc ctgaaatatt atagaggtat attatgagtt
   163801 tttttcaagc tattattatg ggtattatac aaggattaac ggaatttatc ccggtaagca
   163861 gttcggggca tctggtatta tttcagtatt tctttggtat gggagaaagc gaaaatatag
   163921 tttttgagct cttcttacat ttgggaactt tgtttgcagt tcttatttat ttccgtaaaa
   163981 ctctatggga attattcaaa tctttattct gctggggaaa tacagtgaac cgtgaacagc
   164041 atcgtaaaaa cagaacttta gtatattatc taattatctc aaccttggca accgctataa
   164101 tatacattct tgttggtgat tattttaaag ctgcttttgc aatgccaata ctagtagctt
   164161 tctttttatt aattaccggc ggcatagttt ttctttccga ttattttgtt gatcaaggta
   164221 ttcctgcttc aaatgttggt ttctttcgtg ctgtgataat tggtatagga cagggtatag
   164281 ctatattacc tggagtttca cgttcgggaa ctactattgc ttattcgttg gcaacaggga
   164341 tgaagcgtaa ggatgcagct caatattctt ttttattaag cattccagct attctagcag
   164401 gtaacctttc tgaatataaa gcacttactg agttggattc tactatgctg caaaactatc
   164461 tggttggctt tgtatcttct tttataattg gctacctggt gattgcattt ttaatcagat
   164521 taatagaaaa tagccgtctg aagtattttg gaatatattg ttggattgca ggtttgctct
   164581 ctattatcct tctggcatta ggtatataat ggaagcacct attttcgctc ttgattttgc
   164641 tgctgcaaat gttaatttgg gctcttccta tcttcttgtt gggacagatt cctaccttgt
   164701 ggataaagtg atagagacaa tcaagaaaaa attgaagaaa aaggaaaata tagatacttt
   164761 cattatttat ggtgacgatg ttaaaagctc agaactggca gatattttgg atacttacac
   164821 aattttctct tcagcaaaac ttgtgatctt gaaaaatgca gaacaattga acaaatatga
   164881 actgaagacc ttatctagct atttttcttc accctccgaa attcaaagtt tagctatagc
   164941 aacggaaaag atagacctgc ataatgaatc ctggaaacaa attaaagctc attctacaat
   165001 aattaattgt gaatctcctc gttatggagg ggcaattcgt tcctggttgg ataaaagttt
   165061 gaaagatatg aataaaacaa tgactatcaa agctattgaa gaattcataa accgcattga
   165121 actcgattat tattatgctg ctaatgaatt gaacaaactg gaccttttaa ctaccggcag
   165181 gaatagaatt acagaagaag atgttatgaa gagtttaggc actacccgta ctggaacttt
   165241 aatagatttt tatcgggctt taggcaaaaa acaggaaaag caagcgatag aagcattgga
   165301 aaagatgctg aatgccaatt gggaattttt gcaaatattc ttccagttca cgaaatttta
   165361 taataacatc tggcatatat tgctgctgaa aaaggcacat atttccgata atgaaatcgt
   165421 atccaaacat cttagtgata tatatcctaa tcagcgaaaa gaatttttgg aattgtcccg
   165481 ttcctataat ctagcttcgc tggaaaaaat cttcaccatt ttactggaaa ccgatcaaca
   165541 gtttaaactt tcggttgctg attctcaaat tcttctgatt acttgtttaa ttagggttat
   165601 ggaagcataa tgcacaagac aggtatctta ttgcatatca gctcgttgcc ttcagatttc
   165661 ggtatcggag attttggacc tgaagcaatt cgatttgcag aatatttaaa aacagaaggg
   165721 tatacttatt ggcagatatt acccataact cattgcggat atggaaattc tccttataat
   165781 cctctttccg cttttgcctt aaatccctat tttatcagtc cggagctgct ttatcaacaa
   165841 ggcttaatca atcaaagaat gctgaaagca gtaaaaatac ccgcaagtaa tatcactcaa
   165901 tatgaaagag tatataactt gaaagacaaa atgctagctc aggcgactga caactggatg
   165961 ctaaccaata atctggatga ttatatagaa gaaaatgcct tttttttaaa gccctattta
   166021 acttttctat cgttaagcag aatttatgac gatagctgtt ggtataattg gaatgaagaa
   166081 cacaaacact atagcgaggc cttatatcaa aaaactcgtc aattggattc ctccttcata
   166141 aatatcaaag cggcaactca ggcaatgtta tcggaacagc tgaaatactt taaagaatat
   166201 ttgcataaaa tgggaataat tctcgttggc gatttaccgc tttatctttc ctatgatagt
   166261 tctgaggttt gggctcatcc tgagttcttt gatttagacg aacagggaag gcgtttacat
   166321 tttgcaggtg ttcccccaga tgctttttcg gaaaaaggtc agctttgggg taatccactt
   166381 tacaaatggg atgttttaca gcaggatagt tttaagcttt ttatgaatag aattggtatg
   166441 gcgctaagtt atctagacat tttgcgactg gatcatttta tcggttatgt aaattattgg
   166501 caggtaaagc caaattttga cgaaaaagga catccaatac ttccccaaaa tgccataaac
   166561 ggttcttggg aacctgcttt accagaagag ttcttctcca ctttattaaa gaactatggt
   166621 atagagcgtt ttcttgcgga agatttaggt atactaaatc aggaagtatg cagagtaaga
   166681 gatagttttg gttttccagg aatgattatt ttacaatttt gcttcgaaaa tagtgtacct
   166741 gaagtaaaaa aatatcctcc tgaacgttgg ctctatactg gaactcatga taatcataca
   166801 attcgtggat ggttcgagtc gcttccttct gattcactat cattaaagca cttgcaagaa
   166861 tattgcatac aaaataaaat aactgatatc catcagatga tcaatagcga gaatatacat
   166921 aacataatga ggaatattgc cttatcttca ggttgccaac aaatcatatt tccacttcaa
   166981 gatattcttg gtttaggtga cgaagcaaga atgaatgttc ctggtactgc tttaggtaat
   167041 tggcaatggc ggttagaaga aagaatttat taattataaa taaaataaaa aggagaaaaa
   167101 atgccaaaac ttatgctttt gggtgatgaa gcccttgctc agggagcttt agatgccgga
   167161 atatcaggat tttacgggta tccgggaact ccctcaactg agatcatcga gtatgcacaa
   167221 aagtcaaaac aagcagcgga aaacaatatt cacagaacct ggagttcaaa cgaaaaaact
   167281 gctttggagg ctgctttggg tatgagtgct tccggaaaaa gagctatggt aactatgaaa
   167341 catgtaggat taaatgtggc agcagatcct ttaatgaatt ctgctatcac aggagccagt
   167401 ggtggtttga ttattgctgt ggcagatgat ccttctatgc acagttctca aaatgaacag
   167461 gatagccgtt actatggaaa ttttgctatg attcctgttt tggaaccagg aaatcagcag
   167521 gagtgttatg atatggcttt ttacggattc gaattatccg aaaagtacca tatacctgtg
   167581 cttttaaggt taacaacgcg tctagcacat tcccgttcag ggattgttac aaaagaacct
   167641 atccctcaaa atgaaatgcg caaacctgag gatatgtttc aatttatgct tctaccggct
   167701 attgccagaa aaaaatatca gcatcatttg gaattgcaaa aggattttat gagggaatcc
   167761 ttaaattcac cttttaacgc ttataaaaca gaagcgagct caaaaaagtt gggtataatt
   167821 acaactggct tagcatataa ctatctgcga gaagctttta aaggtgaaga aattccctat
   167881 ccggttctga aaataggtca gtatccttta cctgaaaaac aaattcatca gctctatgat
   167941 agctgcgatg aacttctagt tattgaagaa ggtatgcctg ttgtggaaga acaacttaaa
   168001 ggcttggctt ttcctggtct aaaaccaatt cacggacgtt tggatggtta ccttcctcgt
   168061 actggtgaac ttaaccctaa tcttgtagct aaagcatttt ctctgcccga cacaataggt
   168121 agaccagttc cagaaattgt tgtgggtaga cctccagctt tatgtgtcgg ttgtcctcat
   168181 tccgatactt ttctttcttt gaatgaggtt atggctgaat atggaagagg tcatgttttt
   168241 agcgatattg gttgttacac tcttggattt atgcccccat ataattctat caattcttgt
   168301 gtggatatgg gtgcttctat tactatggct aaaggtgcat ctgatagtgg acttttccca
   168361 gccgttgctg ttattggaga tagcactttc tgccattccg gaataactgg attgctggat
   168421 tgcatttatg ataaagccaa tgttatgatc gtaattttgg ataatgccac aactgctatg
   168481 acgggcggac aaaattatac aggatatgga aaactggaag atatttgttt gggtttagga
   168541 gtggaaaaag aacatattcg aatctttgtt cccttaaaga aaaattatcc cgagattacc
   168601 caaatctata aagaagaact tgcatacaac ggtgtttctg ttgttattgc acgtagagaa
   168661 tgcattcaga ccctaaagaa aaagaacaaa atggagacgg tagaatgaaa aaggatataa
   168721 tacttgcagg agttggcgga caaggtatat taaccatagc caccattctt ggaaccgctg
   168781 ctattaaaag aggaatgaat ttaaaacaag cagaagtgca tggaatgagt caaagagggg
   168841 gagatgtgca atctcatcta cgcctttccg atgatcctat ttggagtgat ctaattcctt
   168901 atggctctgc cgatatgatt ttaagcgtag agcccttgga agcattacgc tatctgccat
   168961 atttaaaaga agacggctgg attatatcta attccacccc ctttaaaaat attcctaatt
   169021 atcctccgga agaagaaatc tacagggaaa ttaagaaaat ccctaattat gttctttttg
   169081 acgcagatac tttagctaat gcacaaaaag ccaatcgcag catgaatatt gtagttttgg
   169141 gtgcagcgat aactcatttg ggctttagcc aagaagaaat tgaagaggca attaaagcga
   169201 tttttgctcc caaaggcgaa gccattgttg aagcaaattt aagagctttg ctagccggta
   169261 ttgaaacaaa gtaaacttat tgggcgtagt tatctacgcc ctttttcgtt tctcacagat
   169321 tgcttaagta actcacgctg attacgcaga gagcgcagat tatatttatg taattatatc
   169381 gttccagcta atttagccga tccagttatc gatgttcttt tttttaaact tccagatccc
   169441 tcgacttctt atattggcaa cgaaacttat ctctttccaa aatttctatc tcaccaagtc
   169501 actatctctt ttttacagga ttgacccata agccagtgtt accaaagcca ttattgcctc
   169561 aaagataact ccataatcat catcaatgaa ctcaatggtt cttccatcaa ggcgttttgt
   169621 atgaggcatc agagcagcgc aatcggtcat agaggtagag tgaaactcaa ttttaaggtg
   169681 tatagggctt tgaaatgtat atagaggtat atctttttta gctaaagatg cttgaactgc
   169741 cttcacgaga gcttctttga ccacgataga tgaataattt ttagcagcaa atttagcgac
   169801 tgcttcttta gttctaacaa aatgcaccca gggcatagcc gttgttattt cttcttccaa
   169861 agctttatca ccacttatta aggccaccgg gacacctaaa taaccagcat aagcagaatt
   169921 tatgattgct tcattcatcg gaatatcgtt tatccagatc ttaaaaatct tgctgttaga
   169981 ataagtgtgg tccatatttc ccttcagggc accggttcca gaatgataac ccagtaaaaa
   170041 gaccatagaa tattcgctac taaaggcagg catcatatat tgaggtcttg ggcctccgga
   170101 aattagcgaa attcttttat ccaaagaggt tatatcatag ctcaaattat ctcctccact
   170161 gtgggaatcg gcaacagtga tttcctctat gaattcatta aatccgctat acaaaatggc
   170221 attcaagcag tcacttatat gtgcctgata atttttcata acttccggac ggttggtttt
   170281 ttcctgctcc cagttaaaag ttcctggcat tccttccata tcagtagaaa tatatatttt
   170341 cattttaact ccttggcgat ttgcgcgccg attagtacat tatttttcag caaagcaaga
   170401 ttggtctgaa ctgatttgcc tgcagttttt tctgccaaat ttgccagcag gaaaggagtt
   170461 aattctttac cggaaattct ttttgctttt gcctcatcaa tagctgcgat aatgtgttcg
   170521 gctatttcct tataaggaat ttgatctttt tccggtacag gattagccac caaaattcct
   170581 gtgggattag gatacaattt ttgtatcgtt ttatagatat ttacaacatc tttagcgctt
   170641 tcaattttgt ctatcttttt tccgcttttt tgcgaataga atgcaggaaa ttcattcgtt
   170701 ttccatccca atacaggaac agcttcactt tccaaaactt ctagcgttgc tggaatatcc
   170761 aaaattgctt tacagccggc agaaaccaca attatcggaa tttccgaaag ggcttttaag
   170821 tcagaagaaa tatccaatgt ctcgttccag cttttatgaa ctccgcctat tccacctgta
   170881 gcaaaaaccc ttattccggc tttatagcta agcagcattg tggcagaaac agttgtccct
   170941 ccgctttttt tataatttat tgcatagggt atatctcgca tactaaattt attcagttct
   171001 tgtgaatgct gtagtttatt tttcaggatt tccttttctt gatcatccat tccgatatga
   171061 agttgaccat ccagcacaat tattgtagct ggttcaactt ccaaactgta gcagagctct
   171121 tccaatgtct ctaaaatatc caaattgtcg ggataaggca aaccatgcgt taaaacagtt
   171181 gattccatag ccaaaatggg ctttcccaaa tctaatgctt tctttatgcg agaggagtaa
   171241 tttaatagtg aatgcatctg tagttacctg tcgttattca taacagggtg tcaacaagga
   171301 taggtaacta tattaatgtt gtgcaagtaa agaatctgta taagacgaca tcataatttc
   171361 cacttccaca ggtaaaactc ctgcagcaag catatcaatt tctttagcgg ctgcataaga
   171421 aagatcaata tctctattac gattgaaggg acctctgtcg tttacacgca cggttaccga
   171481 tttgccgttt tgaggattag ttatttttaa aagggtttga aaggggaggg atttatgggc
   171541 acaagtgaag gcatcgcgat tatatctttc gccacttgcc gtctttctac catgaaatcg
   171601 tttagaataa taagtagcga tcattttttc accaggagga gctcccggtg gttcctgggt
   171661 ttgtaaagaa tcaattaccg tcaaacttcg atcagtaaaa aattcttcct cgttcacgct
   171721 tgccacggtc tgagctatga ccggtgctat aaataacaat agaaaagcac taaagcaagc
   171781 tgttaatttt ttcttaagtt tcttcatatt agtcctttgc tcatttaatg tggaacatcc
   171841 tgttcagcag gggggataat gtcaagaaaa aattttcttt acagggttta tttaattata
   171901 tatcaatcag ttatacgatg tgttacttgt ctcttgtttt tacttatttt gatcctttaa
   171961 catatcttta ttgatacctt aacgggttta aagcagtgtt aagtaagcgc taaaaaatcg
   172021 ttaaagaaag gattaagtaa cagcaattat aagctatatt attatgtaag gcaaaggtac
   172081 tttgctatta tatatccgct gcagatgcta tttttacaat taaataattt ctgtattccg
   172141 taaggttgat gtcctcatca attatttttc atttgttatt tggcaaccct aaaattcata
   172201 ataacgataa agatgggtta caaatagaac tacaagatgt ctttgttccg gtttaataga
   172261 attggcttgt aagagttata agaactacct acaggagatt acaaatatcg caaagaaaaa
   172321 aatggtgata aatccaaaaa tatataaata ggagttattg aaacatgcat agggaatgca
   172381 accattatga aaatcaatat ggaaataaca atattcacgg taggcaagaa gtcaagacat
   172441 atcgactaca gataagtgga gtaaagggaa aaagtacctc tctttttatt aagtttcatt
   172501 cctaaggaag aagtaatata acttttttac aattcatcag aaatttccct ttatctaaaa
   172561 aatccgaata agaaagccac atagtttatg tacatatacc gcaacttagg tgctgaataa
   172621 aattactcta aagaggggca agctcatagt tcgttagcaa acttcggttt atttgccaag
   172681 caagaactgg taagctcatt tacctcttaa ctttaaaatt atgggttgat attgattact
   172741 atcctaatat atttacctac cataaaatgt ttagtacctt tgtataaact gcatatcagg
   172801 ttcaacttct actactcgct cttaggtaaa agcgttaatc aagtgtttat ctaaccttaa
   172861 tcaagcgtta attattaacg cttgattaag ggatgttaaa cgagagatta aaacctccca
   172921 gcaaggaaca taggaaacaa caatattagg attttattat aaaagtgatt gttgaggttg
   172981 gtaaaataca atttcaatag aaaaataatc gcaatatctt tttgttgttg ctttaatcat
   173041 ttcaattgtg attgatattt atatgtatcg taataggaat ggaaacttaa gagggaggta
   173101 attttctact tttacagccc atacacaaca aagaaatagt ttgacagaat accgctgaaa
   173161 atctctttat cacataaaga gaaaaaataa aaaaagcgag gtattcgata tgccaaaaat
   173221 gactgttgat ccagattact gcaaaggttg tggcttgtgc ataaatgctt gtccgagaaa
   173281 gattattcgc ttatcggaaa atattaatgc caaaggctat aattatgcag agtgttttga
   173341 gcaggaaaaa tgcactgcct gcaagctatg ttatataacc tgtcccgatg tggcaataac
   173401 gattgagaag tgaggttatg aatgtccaaa atattaatga aaggaaatga ggcaattgca
   173461 gaagcggcta tccgaagtgg ttgtcgcttg tattttgcct atcccatcac cccccaaagt
   173521 gaattgatcg aatatatggc aaaaatgatg ccgaaggtaa atggtacctt tattcaagca
   173581 gaaagtgaag ttgctgccat taatatggtt tatggagctg ctggaagtgg aaaaagagtt
   173641 atgacctctt cttcctcacc aggtatttct ctaaagatgg aaggtatctc ttacatagct
   173701 ggagctgaac ttccctgtgt aattgttaat gttcaacgtg gtggtcccgg tttaggtgat
   173761 attcaacctg cgcaaggtga ttattttcag gcaactaaag gtggcggcca tggcgattat
   173821 cggttaattg ttttggctcc aagttcagtt caagaatttg ctgatatggc cagtgaagca
   173881 tttgatttgg cagataaata tcgtaatccc gttatgattc ttgccgatgg aatgttaggt
   173941 cagatgatgg aaccggtgga atttaaaccg gcaaagactg atgcggaact acaagaactt
   174001 gccaatcaac atctttcctg gtgtatttgt cccaatgcag atggcgataa aaaacaccat
   174061 catgaaataa attctctgga acttgatccc caggttttag aaaagcatgt tttagattta
   174121 tataagaaat atgctgaaat cgaaaagaat gaagctcgct atgaaactta taatgtatct
   174181 gatgataatg aagttctatg cgttgcctgg ggaactgcca gccgagttgt aaaatcagcc
   174241 atcaatgaag taatgaaaga agggaaaagt gttggcttga ttcgtcctat ccgggtttgg
   174301 ccctatccgt atgatgccat caaacaggca ataggtaaaa atgtgaaaaa agtgtatgta
   174361 tttgagctaa actcaggaca aatggtggaa gatgtaaaaa tagcagtaga aggaaaggtt
   174421 ccagttgatt tttggggaaa attaggtgga gttgttttta ccccagccga aatcaaagaa
   174481 aaactggaaa cttgttttta aaggtaggag gaaaaaatgg aaattatcgc aaaacgtcct
   174541 gaagcactta ctaaagaacc cttccattat tgtcctggtt gtatgcatgg tgtagctcat
   174601 cggttaattg ctgaagcaat cgatgaaaat ggattaataa atgaaatagc tggtgtagct
   174661 cctgttggtt gtggtgtttt tgcctataaa tattttaatt ttgatatggc agaggcagct
   174721 catggtcgtg ctcctgcagt cgctacagga ataaaaagag ctcgtcctga tatgcatgta
   174781 tttacttatc agggagatgg tgatttagct gctattggaa ctgcagaaat tgttcatgct
   174841 gcaaaccgtg gagaaaagat aagcgttttc tttatcaata atgctatcta tggaatgaca
   174901 ggtggacaaa tggctccaac aactttacct aatatgaaaa ccactaccag tccttatggt
   174961 agaaatgttg aagatattgg ttatccaatc agggtttgtg agctattatc tacactggtg
   175021 gctccttatt acattgaaag agtttcactt ttaacctctg ctgatattat taaggcaaag
   175081 aaggcagtag aaaaagcgat tcgttataat aaggaaaacc gtggttttac ctttgtagaa
   175141 tttattagta cttgccctac taattggggt tatgaacctg aaaaagcaag gcaatgggct
   175201 aaggaaaata tgctgccttt cttcaaacca ggtgtttttc gtgataaagg tgaaggagtg
   175261 gaataatgac tactgaaatg atatgtgccg gttttggcgg tcaaggtgtt cttacgatag
   175321 gcaaatttat tgctcaagca gctatgcaag aagggaaaaa tgtttcctgg ttaccatctt
   175381 acggacctga aatgagaggt ggaactgcta atgtttcaac agttgtttcc acagaaccaa
   175441 ttgcttcgcc aattgttagt tttcctgatg tattggttgc cttgaatcaa ccgtcgatag
   175501 ataaatttgc ccattctatt cgtcctgggg gagttctgat ttataatact aatatgtgcc
   175561 ctcggggctg tcaacgcgaa gatattattc aaatagcggt tccgatgaat gaaatagctt
   175621 cagaaatcgg cagtatgatt gttctcaata tgttggcaac tggtattata attggtaaaa
   175681 ccaacattat taagtacgaa actctggaag gtaatttaac ttccttcctg aaagaaaaga
   175741 atcccgattt acttgcaaag aatcttgcag ctattcaacg gggtattgag attggtaaac
   175801 aatacgctta aataacacta taataatacc aggaattgga gagtctctat ctttccaatt
   175861 cctggaatac tttacatttt agttaactag ggggaagaga caatggaaga aaaagttata
   175921 gttactgtgg ctacctacac taacctcttt gaagctgaat tggcaaagag cttattggag
   175981 gattctggtt ttcatccaaa tttactaaat gaacgaatga tgggtatgta tgcttctatt
   176041 gctggagata tgtacaaaat tgaattacaa gttccggaaa gtgaagaaca ggaagcaaaa
   176101 gaattgataa ctggtctgga ggattctgat tatgtaacaa gaatcctaca aagtgaagga
   176161 gctcttttgg atggccattt tcaattaacc tcaggcaaac atagcaatca gtatatcgaa
   176221 aaaataagaa ttctgcaaaa tcctgaagca gctcataatc tttgtaagcg tcttgcagaa
   176281 catcttgttg aatatgaatt tgatgccgta gttggtcctg cttatggagg aattgctctt
   176341 gcctttgaag ttgccttaat aatgaagaaa aattttctat tcacccagcg aaaagatggc
   176401 gaaatgactt tgcgaagcgg ttttgacctc tcaaagctga aaaaagtagc cattgtggaa
   176461 gatattctta ctaccggcgg aagtattcaa gaagttatat cctgtcttga aaataggggg
   176521 ctggaagttg ttgctgtagg agttttagtt gatcgcagtg gtggaaaagt tcaattttct
   176581 gtcccactgg aatctttgtt ttgtattgat attccagctt ttaatcctga taattgtgat
   176641 gcttgtaaac aaggtttacc attagtaaaa cctggtagca gcgataaagt ttcattctaa
   176701 tcttatatgc ccaagataac gattgtaaca cttctacctg gcgaaattga aaaggttata
   176761 gatgtttgca aattctctcc gataaattca gagctggaaa ttgagattat ctatcctcag
   176821 aaagatgttt cttcccacca agcaggaaac ttaaaactaa caaaattcga ggatgttcag
   176881 gaacttcctg ttcccgataa aagtcatatt tgtttttttg caagtcctat ttcctgtgat
   176941 ctgtatcgta aacaggcaaa aataaatgta tcttctctct atgtgcatcc agaactggca
   177001 atattacaac ttcaaagcga taatccattt aaaacaaaac tttctgatta tggtttatcc
   177061 ccttgtgttt tggagggaga tgcagctgct gatttatata ttgagcaaga tagattaata
   177121 tatttttgtg atttccaaca gtttatgcag aataaaaagc cgaattttgt tctgctaagc
   177181 actaatactc aatttgtgga atcccttata agtatggata gtgcacattg ggaacatttt
   177241 catccagaat taaattatcg taatctctgt gttggataca ataatcctca ccggttattg
   177301 ttacattccc aaaaacagct actaaggtta attcgcatac ctgatagcat ttatgattta
   177361 ggagggatcc gttattctca acaggatgat tcaactgcct attctttttt tgcaacccac
   177421 tatgatcaat atatggctca tgtggattat gaatcatggg tgaattatgt tctttcctgg
   177481 tttaagcaat attcctcttt acagcttaat aagatcttag agctcgcttg cgggacagca
   177541 aatttctcag aacaacttgt aaatagagga tatcaggttt atgcttgcga taattctatt
   177601 gatatgttgc atatggcagc taaaaaagca gtaaaaccta tattatatta tgcttcttta
   177661 acagatccta ttcccggaag gaattatcag ctgataattt gtatgtttga tagtatcaat
   177721 tatctcactc aggcaaaaca aataaccaaa acactgaacg aagtttcgct ggctttagaa
   177781 aacggaggtt tatttgtttt tgatatttct actctggaaa acagtttaga caacttttct
   177841 gatgtctctg aactatacca ttattctgat ggtttaatgc ttcatcaagc atggtttgaa
   177901 agtgtgcgat taatgcaaaa atctgcactg cactttttta aaaaggatac aattggctat
   177961 tgctatcaat atgaacaaca cttacaaagg gtttatttta ctaatgaact gattgagctt
   178021 atccacaatt ctcctctgaa actaagagct attcatagca cggatactaa aacaaacttt
   178081 tatccaaaac acttaaacag catagatgaa aaatatagtc gcctgttttt tatcctggaa
   178141 aaaatctgaa aatgacaaac ctctatcaga atgatataaa acatcttacg gaaaatggtg
   178201 tctttgccgg aattgatgaa gcaggtagag gtgccttagc tggaccggtt gttattgcag
   178261 ctgtagtttt agattataac aaaattattg aaggcctaaa cgattccaaa caactttctc
   178321 ccaaaaaacg cgaaattctc tttcagcaaa ttgtggaact tgctcttgat tatagcattg
   178381 tagaaatagg cccagactac atagataaac acaacatcct tcaggcaact cttttgggtt
   178441 tcaagaaagc gtttttacag ctgaaaagta aaccggaaca ttgcttgatt gatggaaaag
   178501 atatacacaa agacctgaaa ccttatgcaa aagcgattat taaaggtgat caactctatg
   178561 ctgcaattgc tgctgcctct attttagcta aagtccatcg agataaactg atgcttgctt
   178621 atgatgaaat atatccggaa tacggtttcg cctcccataa aggatatgga actgctttcc
   178681 acagtaaaag aattcatagt ttcgggctat ctcctattca ccggaaaagt tttaaagtac
   178741 ctgaggtcta aaaggctgtg aggattatga agttttggga tataaaccca cagaataaat
   178801 accataataa tgcggtgact cctctcatta aatatccaag agaagacacc gcatatctat
   178861 caaagagaat taaacaggaa tcaagctaaa ctgcgttctc tatcaacatt tagtttttag
   178921 ctcggagtaa ttcttttctc tctttgcttg tatggcttta gacttataca agtagttttg
   178981 gacttagctt tcttaaaccc ttaatacgaa agggctattt gaatgcttcc tgaccggtaa
   179041 actgacggta gggatcaaaa ctctttagaa cttctttatc cttttctgta gtgctttcta
   179101 aacagatacg agtaactaat tcacccattc caggaccaag cataaaaccc tgaccacaca
   179161 ttccaacagc attaatcaga ttttctgctt catccataat tcccactata ggaaatccat
   179221 ctggggtcat aggatactgt cctctccagg ttcggcgaac ttttagatat cgtaaacggg
   179281 gatagatagt tagcattctt ttacagcaca taggtaaaaa gagagaagtt gagcgggtat
   179341 caattccaga aataggaggt tcaggagtta tgcaaaaaac aacttgtcct tcactattct
   179401 gatagaaata gaaattagaa cttccaggtg ctacacgcat atcaataacc atcgggccaa
   179461 aaaatctttc tactggttca gttatgcctg cttcatggtt ttcgggaaat acaggtaaat
   179521 ccagattagc catttgacca acttcgcgag cataacttcc tgctgcatta acaactttag
   179581 cagaagaata aatgccttta tctgtgatca cttttgtaat tttactggaa acaatattga
   179641 atccggtgat atgttcctga aaatggaaat ctacaccttc ttctttacaa tgctcaaaat
   179701 aagctgcaga aacaagtaaa gaagagcaag agccatcttc gggagaatag gtagaaccct
   179761 gtaaaccctc tttccagatt ccgggaacta tatcactata ttcatcaggg tttagccatt
   179821 ttatattaag tccaaaaccg agctgaactt gcatcaggtc tttcaaggtt ttggcatcag
   179881 tttcattata agctggataa ctgtaaccat tggaaagcca accaatatct tcgcccattt
   179941 cttctgacca gttttttaat atctcaattg atcgtaaaca tacatttatt ttaccaaaat
   180001 cagaatgggt ggcacgtact cccccaatag cttttttgtt atttccctgc ccagggctat
   180061 gttctgcttc tataactaag acttttaatc cagctttcgc taaattataa gcggttggga
   180121 gtccagttga acctgctcca ataactatta catcatatat cttattcatt ttgcactccc
   180181 gttgggaaat ttctccagag gaatttctat aaataacggt cttttagtaa tgggagcaat
   180241 ctctttttct ggaatgcctt cttcacgaag taaacctttt atcagtacct cacaagtttt
   180301 agagccacaa ggtcccattc cggctctggt tatagatttt atcaaattca tatctgtaat
   180361 tccttttctt ataagagctc gtacttcacc tgctgtaact ctttcacata aacaaaccat
   180421 tgcttcatca gctgtctttt ctaaaagaac tggttttgtt aatggtgcag aaacatttgc
   180481 gtcctgaatt tggaatccag caactttttt agcaattgct tttggaactt taaattttac
   180541 aatctgagta tgttgtttgg gaaaatcctg aactgcagta atagcatatt tacctaactc
   180601 cttggcattt atatcgttca ggattttatt atcccctact ttaacaggat aattgcttac
   180661 ttcgtataca atactgatag ttggattttc tttgtctttt ctatagtcca ctaaagtaat
   180721 agctaaacct ggacaaatta acaaacattt tccacagcca atgcaagagc cagtataaac
   180781 aggaacagct gttaaagaac catcctctgt gtgaatacta ttggtagggc aaacagttgt
   180841 gcaaggatta cagggaattt cctgaaggca atgcaaaata gggaagaccc cttcctcttt
   180901 ttcgggagta ttatagttat ttgtttttcc tggagctgat ttcagctcct ctgcttttat
   180961 aaaccagtca tcaggaatag gattcttttt ttctcccttt aggatggaag cgattttcac
   181021 accggctatt tttccattga acattgcaga acttgcttct gcaatttcta atgcatcgcc
   181081 ggctgcatca attggtaaac ctgctgcttc tgcctctaaa gtgaattcac ttaaaggatt
   181141 aagtcctact gcaattaaaa ttgtatcaca ggtaaaggtt ttttcagttc cttttacagg
   181201 ctggaagttc ttatctaatt ctgcgatagt aatactctct actgtatcat ctccattggc
   181261 acaaagaatg gtatgggaag tatagattgg tactccaaag cgtttcagtt tatcagaatg
   181321 aactttataa cccccacaaa caggcatcgc ttctgctaag ccaacaacat caatacctgc
   181381 ttgcaaggaa tggtaaccag caattaaacc aacattgcct cctccgatta taaaaagccg
   181441 ttgggtagga cgtacgagat cccgatttac aagtgtttga aaagcacctg caccataaat
   181501 tcctgctaaa ttgttacctg gaaatcttaa gaatttctct ctggcaccag cagcattcaa
   181561 aatacgtttg ggttgaacaa ttttataaac tccatctttt agaactccta cctttttgtc
   181621 gctgaaaaca aaaacagcag tactattcag ccatatatct acactgctat atgtagcaat
   181681 ttctgctgca agatttttgc caatatcaat acctcttgtt cctgcactgc tatctgcttg
   181741 agaaccaaag aatttatgag tttgtagaac aagcttgcca cctaaggcat ttttatcgtc
   181801 aataaccaag gttgggatgt tttgtttgcc gagttctata gctgcagaaa gtcctgctgg
   181861 acctcctcca ataataagaa catcggtatc aatttgttcc aacggttcaa acgatagagg
   181921 gttatctacc gcaggtaaaa caggaagccc ttccaatgat tgcacgatca tattttcact
   181981 gacagctgtc atacaagatt ttacagctaa accattagct atcacactac attgtgaaca
   182041 ttgtccattt gcacaaaaaa ttccttgagc actgccatct ttatggtgat gcccaaaaac
   182101 cgaaatacca ttggcaataa gagctgaagc aattgtttct ccgcactttg cctgcatttt
   182161 ttgcccattc cagtaaaagt atatttcttt tttatcagga acaggcaaaa tgggatgaat
   182221 ggcgatacga ttgctatctg ccatttgttc aaactcctta ttctttaaag agatatgttg
   182281 ctttacgagt cagcagagca tattcccttt ttctaatatt atttatcttc ccttcaattt
   182341 tttccttggt agtttgtcaa gcagtaattg tggcacattt agcagtagca cttgttgttt
   182401 tttatatcta cagcaagata gatagctcca attttataat ctattgcaga aataagaagt
   182461 ttaagcaaat tctaactgca ttcccattct tctaatgact gttatggtaa aaagcactac
   182521 agaattgcta aatttctgct ttatatatga atcctaaacc aaggatagta aagaacaaat
   182581 tcgggaacca ggcagcccaa acaggattaa ttatactatt ataacctaaa ctctggcaga
   182641 cccttacaac aattaaatag acgaaacaaa ccaccaagcc cagcataatg atccaaccgc
   182701 gacccttaga acgaatatta gaagtagcta tggggataaa gaaaaagatc acaattaggt
   182761 tagtgagcgg aaaggatatt ttcatatgta gatctacaat ttcacgggct ggatcatcac
   182821 ccagcttttt catcctgtct atatattctc tcaattgcca aaaatttaag gatagggttt
   182881 tttgcgtaat ccttataaaa tcctgaggtt gaacatctaa attgacaatt tcagtctgag
   182941 gataataagc cataaaaact tgtttgccat tatcaaaatg cctaatctca caatctctta
   183001 acacccatct acttccattc caatcagcag tagaggcagt tatgtgttca cttatttgat
   183061 gagttttata gtcaatacga gtaaggtcta taatttttag attgttctta taaccatcaa
   183121 aaaaaccaaa gtaataaaaa tcgttcgctt tgccctgata gtgtattctg gctttcagca
   183181 tttggtcgtc tggctgctgt ccctttattt gaacattgta aacataattt ttggtggttt
   183241 cagcccaagg gactacatat tctccaaata gagcaactgc aatactaata ataagaccaa
   183301 taccaaaaag aggcaacata gctcttttta tactaattcc agcagcacgg atagctatac
   183361 tctcgttatg tttagaaagt gcactcatca taaataaacc agttagcaga actgttactg
   183421 gagaagttaa gacaagcaaa taaggtaacc gcattaaata atatatagtt gcctgttgag
   183481 tggtagcacc gttacgaatt aagcgcggca aattatcaat aacatctatc accacaaaaa
   183541 tagcgacaaa agatagaaag ataatcagat aggtgcgcaa aaattcacgg ataatatatt
   183601 tatcgaggat tttcatcttt agtgtacaat ctcatctgga acgggtgctt ttctgttacg
   183661 gtagtgctta attctccaca tcagagtggg catatctatc aactttttct ctttaatgga
   183721 gctaataatc aaaaaaatgg caatgattaa aaacgcaata ttggataacc acattgaaag
   183781 aaaaggtggc atatttcctt tgtcagcaag ttgttcgcct gcattcaaag ctatataata
   183841 aattaggaaa attaccgaag aaaccgaaaa ggacatacct atcccactgg ttcgagtcat
   183901 taaaccaagt ggtataccta tcaagataaa gataatgata gcgaaagaga tagaaaattt
   183961 cttatgatat tcaacctgaa gagagctaat cagttctttt agttcttcta ctcggtcact
   184021 tgtcattttt tgcattgaat gcaagcgttg taattccata ttgcgagtaa agcttggagg
   184081 gcttttctta agcagttcta tgcgtttatt caattctact atttcttgtt gttttattgt
   184141 aagttcctct ttgtgacctt taatagcagc aactaactgg ccatagttca tttctctatc
   184201 gctacgataa cctgtttcaa acatatcaac attgctacct aaattacgaa catttatcac
   184261 atagttggta aaagtggttt tctgatattt accgggctct ttttcattgc gttcatgcat
   184321 ttctccgtta tttaatatta tctgaagaga attgccatta tccttctgaa ttacatttcc
   184381 tgttttggca aaaacagtac gggggaaatg tgtttgactg cgatcatata ttagaacatc
   184441 cgttagtaaa gaatccgttg cttcattggc gaagatagta taatccataa agttcgtaaa
   184501 ttctttctct ttgataatcg tcattggctt ataataagca attttcagtg ttaaattctt
   184561 caatttatga ttagtttcag gaagaaacca atggttaaaa taaaccatta acccggttaa
   184621 taacagagct actataacca aagggccaat tagcgaataa atatttattc cgctggattt
   184681 aatagcgata atttctctat ctacactcat tcttccaaaa gcaagaatag ttgcaacaag
   184741 cacagccata ggaatagaaa gagcaagcat ataaggcaga gaaagaccaa atagttcaaa
   184801 tattatttgc caaggcagtt tcttttcaat aatcaggttc aataaatcga taattcgatc
   184861 actcaataaa acaaaggtaa ctaccatcag agcaataaaa aatggagcaa tatgctcttt
   184921 caaaagatat cgcttcagga ttttcatttc agcatatcca aaagctctaa ttcattaatt
   184981 attttaactg ttcccagttg gtgtgcttta tccagtttag aaccaggatt ctcacctacg
   185041 ataaggtaat tgagttgttt gcttacaccg cttaatattt tacccccgtg ttccattatt
   185101 aagctttcca tttccttcct accataatta gacagagtac cagttatcag aaaagtctta
   185161 tttgccaatg tattagacct ttgtgagctg gtataggcaa actgcaatcc gactgctttc
   185221 aaagagtcaa tcagttccag attgcttttt tgagagaaaa agccaataat ggaatgagct
   185281 ataatagaac caatatcagg gacacctaaa agatcttcat cggttgctgc aattagagca
   185341 tcaatggttt taaaatgttg agccagtgtt cgagcagtaa ttagtcctat atgcctaatt
   185401 ccaagagcaa aaaggactct atcaaaattt ttggttttgg atgcttcaat agctttccgt
   185461 aaattttctg cagatttctt tcccattcta tccatagagg caattttgtc ataatcaagg
   185521 tgatagatat caggaatact ctttatctga cctgtttcta tcaaacgggc aattaaagaa
   185581 ctacctaagc catatatatc cattgcctca cgagaagcaa aatgttccag ttttcgttgt
   185641 acttgagcag gacaattagc atttgggcag taaattatgc ttccatcttc atcgcgttct
   185701 aaaggtgtat aacatactgg gcaactaaca ggaaattcaa ctggtttagc attttgcggg
   185761 cgtttactga tatttactga aagaatttta ggaataatct caccgctttt aactaccaaa
   185821 actgtgtccc cataatgtaa atcgagtcgc tttatttcat cggcattatg caaagtagaa
   185881 tgagaaacag tgcttcctga aatatataca ggttttaaat ttgctacagg agtaactgca
   185941 ccggttctgc caacttggaa gctgactgat tccaatattg tttctttctc ttctggctta
   186001 aatttataag caattgccca tttgggactt ttagccgtcc aaccaagctt tttctgatct
   186061 tccagattat taaccttaat tacaattcca tcaatatcat acggaagagt ataacgttcc
   186121 tgctctactt gcaaacaaaa ttcttcaatt tctttatagg aattgcatag agctctttta
   186181 ctggttttaa agccaagctc tgttaaaaaa tctaataagc gtgtttgttt atctaccact
   186241 tcgtttttgg aaatcaaggg tgattcataa taaccaagag cataaaacaa agcttgcaaa
   186301 tggcgagatt taacttcttc agaattttta agtttgattg aaccagcagc agcattacgc
   186361 ggattagcaa aggtttttag ctcattttct cttcgggttt cattcagtgc taaaaaatcc
   186421 tggataggaa tatagatctc acctctgatt tctacgggtc ttttataatc gatagtaaaa
   186481 ggtacttctg gaatcaaacg gaagttctct gttacatcct caccttctaa accgtctcct
   186541 cgagttgtag catatatgaa ttctccgttt tcgtaataca aattgatgct gaatccatca
   186601 attttaagct cagcacaata ttcactatta taacccaaat cattatttgt cctgttcata
   186661 aacgataaaa cctcttccaa ggagtaagca ttttccaagc ttgtcattcg taccttatga
   186721 ggaatggttt tagctccagg agctagatca ctgccaactt tccgagtagg ggattcttct
   186781 atggtttctt cagaatattg cgcttccaaa tcctgcaaat gcttaaccag catatcatat
   186841 tcgtaatcgc tgatttcagg ttttgccaat acataataaa gccggttatg tttctctatt
   186901 tcagctctaa gctcttctat ctcggctttt atttcttctt tattcataag cgataaagct
   186961 ccggtcgtct atctttaaaa gcatcattcc gttctgttat agttttgtca tttgcctcaa
   187021 ggggatttat ttctgcagtt gcaattcctt ccttgttaac aggaagattg gcaagaagtt
   187081 ctccttttgt attaagaacc tggcttgcac ctgtaaagct aattgtaact ccagcattgg
   187141 tttctgtccc tatccgattt gaggttatgg taaataccct attttctaaa ctgcgaattt
   187201 tcattgctgc ctgacaccaa ggcaaaacta aattcgttgg atgacaaata atctgagcac
   187261 ctgatagggc aagagttctt gctgcttcag gaaattgcca atcgaaacaa atcatcattc
   187321 cgatttttac tccagcggga gtagtaaaaa cttgaaaacc tgtatctcca ggactgaaga
   187381 acagcttttc acggttgaac aaatgggttt ttcggtagtt atggtaatca ccatcgggca
   187441 ttattaacat gctggaattg tatagtttat caccatcaac ttcaggataa ccataaacta
   187501 tgctgcagtt attctttgtg gctatattag ataaagcaag aaacgcttct cctttaccag
   187561 aaacttcagc aatggaatat acctcttctt ttgtgctgaa aacataaccg cttgtacaaa
   187621 gttcaggcat aactaccaga tcggttttta gagagcttag caagtctgtg atacgcatta
   187681 agttatatgc cttatccaat aaacgaggtt caaattgtaa aactgatact ttcattgttc
   187741 ttttttctcc ttcattaatg gctgaatagt tctatccaaa acacctttca gataagaaat
   187801 tgtcaatccg taattagtaa taggaacccc gcgcctatgg cattccataa tgcgtctgtt
   187861 catttccata gatgtaatca tacagccacc gcaatgaaca caaacagcat attcttccaa
   187921 attgtctgga aaatcgtgtc ctgcataaac ctcaaaagta agagttttac tgctaaaact
   187981 tctcatccag ttagggattt taactctgcc gatatcgtct ttttgaatat gatgagaaca
   188041 ggcctcagca attaaaatct tatcaccatc cttaagtgat tttaagcggt ttatgccttc
   188101 caaaagaata tctaattcac ctttataacg ggcaaaaaga atagaaaaag tggttaaggg
   188161 aatttcttca ggtacgattt tagctaccat atcaattact tgggaatctg ttattaccag
   188221 ggaaggtggt tcttttatgt tttccagagc tgactttagt tctgtttcct ttacaacggt
   188281 tactacacaa tttcggtcca gcaaatcacg cagagtttga acttgaggca gaataagacg
   188341 acccttgggg gcagaagaat caataggagt aaccagaatt atattacttt ggggatttac
   188401 tagatcagcg gcaataatac gattgtcttt taaaacctga ggtgccaaat tgatcagttt
   188461 atccttacat tctctaattc cgtctttagt tatgcaagat acactcacat aaggcaggga
   188521 tttctttagg cataaatcca tattttccgg gtttgcttct attatgtcac ttttattaaa
   188581 aaccaccagt attggcaatt ccattccgat aattttatcc atcattgtca gttcagcttt
   188641 agtaaatggt ctgccatcag aaacaaaaat ggcgatatca gcacgatata gtattttttg
   188701 agtggcctga acgcgttttt gtcccaattc acctgtatca tcaactccag cggtatcata
   188761 aaaagtaacg ggtcccaaag gtagaagttc ataatgctta tctacgggat cagtagttgt
   188821 gcctgggatt tcggacacaa tagctatttc ctgccccaca agagcattta ttaaactgct
   188881 tttaccggca tttcgttttc cgataatggc tataattaag cgttcaccgc gaggcgtact
   188941 gctcattttt ttactccata ttctaatata cctattcctt ttcttaagaa cttaggtatt
   189001 agtcaagtcc tttctttgct tattacttta ggaagtagaa aagttattga tgtcagcaag
   189061 tttaattaat gcaaaggtta ttttacctgt atatatagtg attagcaaaa aacatcaaag
   189121 ctgataggaa tgtaaactac gcaaaatgta atgggaattg gataagtgct ggcaacaaat
   189181 tatactaaaa taaagggacg cacctctttt agtttaattt cgctaatatg gttggcaaag
   189241 ttatctgcag ccttatgaag ggcatctata tcattgttgt tttctataac taagtctacc
   189301 aaattgcgtt tattaatatc cggtatttgg tgcataattc ttttttctgc cttcgatttt
   189361 tcttcacctt ttttttctaa acggtctaaa cgtttttctt tagaagcact aatcaaaata
   189421 agataatcaa aacaatcttg aagatttgct tcaaaaagaa gaggaacttc aaagcagaga
   189481 actttttctt ggctttgttc agctatttgc tgaaagtcct gtaagacaag agggtggatt
   189541 aaggagttta aataattcgt ttctgtttca ctaataaaaa ccttgtcagc caagagttta
   189601 cggtttatag taagaacatt tttatctctt acggaagtgg ttaaaatgtc tggagaatag
   189661 cgtgttacca aagcactttg aatgtcttta tcttccagat gttggtttgc cagaacatct
   189721 gcagagatta ttctcaagcc attagcagcc aggtaattac agaaagaact ctttccgcta
   189781 ccaatatttc ctgtaattcc aataagcaaa gggcgattat ttagttgcat cggttaaaat
   189841 ctgcatcaat tcttcaaaag cgacattcgt ttgaattttt ccagcgttag ctacagatat
   189901 ttgtccagtt tgttcagaaa caataatagc tattgcatcg cttatttctg taattcctat
   189961 tccggctaaa tggcgagtac cgaatttacg aacatattca tcttttctcg atagaggtag
   190021 cactacttta gcagccatta tacgctggtc gcgaataata attgctccat catgaagcac
   190081 gcttttagga ttgaatattg taagaataag tcgcattgaa acagcggcat caataggttc
   190141 gccgctatat atatattcat ttaatttccg tttattttcc agcacaatca aagctccgat
   190201 tttacgaaaa ctcatagaag aaacagcatc aataatagga gtatagaggg aggatttttc
   190261 ctgtttactg aatgctttac ttaattctcg cggtagattt aaatgagata aaattgaacg
   190321 tagttctggt tgaaagagaa tcacaactgc tataaaccaa tagttacgga tagcgttcag
   190381 caatgaccca accatcttaa gattgaatat tatcgctaca atatagagga caagaacaaa
   190441 cagtaaaccc cataaaacct gataacctcc ggacttacga acaattagca atgcttgata
   190501 tatcaggaaa gcaataaaca gtatgtctac aatgtcttga taacgaggaa ttaaaaattc
   190561 cacttaattt tttccagcct tggcaattgc tattaaaaca tcaaagaatt gtttatgagc
   190621 cagcacatca tgcacccgaa taatatccat ttctccacag gcagcagcaa aagcagcggc
   190681 aagacatcct cctatgcgtt gcgagggaag tgagggagaa attttatcaa taaagctctt
   190741 tctgctagca cccaacagaa caggtaatcc aaattgtttg aaggatttca aattggcaag
   190801 gatggttaaa ttatgctcca gggtttttcc gaaaccaatt ccaggatcaa gtattatatt
   190861 ctcgagcaga attcctttac ttaaacaata atctatccgt tcctgaaaaa agtcattgat
   190921 ttctgctatc acatctgtat aaaaaggatt gagttgcatt gtgcgtggaa ttccttgcat
   190981 atgcataaga acgactttaa tttgcggatt gtcagctaac aattcagcca tctcagaatc
   191041 ggttctgaga gcagaaatgt cgttgattat agtgacgcca agttctatgg acttctttgc
   191101 tacagcagta ttttgagtat ctaccgatag aatttgatct ggatactttt ctttgatagt
   191161 agtaataatt ggaacgattc ttttcaattg agtttccgag tttacaggca aagaaccggg
   191221 gcgggtagat tctgctccaa tatcaattat ttctgctccc tgagcaatca tttctattga
   191281 atgtttaatt gcggaatccc tatctaaaaa ctgttctcca tccgaaaatg aatcctcggt
   191341 aacattcaag atacccatta taacaggaat atgttcagga tagtaattct tattcattta
   191401 tcacaaacct gcaaagtaaa cgataagtag ttttaggatt gtatacacct ttaaatccga
   191461 acttccagga aggcataact ttacggattg attctacata agctgcatta gtaaaggaaa
   191521 gaatattaac actgttcata tctatcgctc catttctcat aagtttaaat tctatataaa
   191581 cctcaccttc ttcggtagga attatttggg gtttggattg gctgataata tatgcttctc
   191641 caccaccctc ttctaaagtt gcagaaaagt ttggatttgt tttctggaaa gttcctggta
   191701 tttcttttaa agaatgggct ccacgattac gggtaaaact ggaaggaata ttagctttat
   191761 tttcttcttg attgggaaca tttaaaggca tagtgataac agattcactt tcaccagcgc
   191821 gctcaggagc taatttgttc agatttaagc tcatatttcc agagctttcc tgggatttgg
   191881 aaattccagc tgatcctgca gcattttttc ctccaatcag gtcttcaatt cttagggaag
   191941 aagttttttt tactttgttt tcttgcgaag attcccattc aaagctatac catttttccg
   192001 gagtgtatgg cttaagaaaa tagaaggcaa aaaagaggaa taacagaagg tgaaaaacaa
   192061 cagagataaa atatccgtta aatagcttga aaggattaaa gcgacgcact actatccctt
   192121 ttctactgta gtggcaataa agatatgttc gtagccgatt tcacgaacaa tatccattag
   192181 gtcaattaat ttttgcactg acacttgttt ttcgacagca atacggacga cctgctcagg
   192241 agtttgatat ttttgctgta atatatcttt aagctgctga atagttatgg gtttatccat
   192301 aaaatacaga tgtccatcat tataataggt gattaataaa gtttcataac tttggcgtac
   192361 cgaagttgaa gaaccgggaa gccgaattgg aaggcctgtt tgactaacaa aattcgagcc
   192421 gattaataaa aacaataata gaaggaagat gacatcggtc atcgatatta gcatagttgt
   192481 ggaaatccgt tgtttggaaa cccgcagttt catagttttc cttgcattat tttcttgtat
   192541 ttaatgatat gttcaattgc attatcctgc atatctttta ctatgttttc aagtttttgg
   192601 actaggtcgt tgtaaaaaat gatggtaaaa atacctacaa tcaatcctcc tacggtagtt
   192661 aacagagcaa cccagattcc tcctgcaagc atagaaatat ctactccact ttgattcgct
   192721 gcttggattt tcataaatac attcaccatt cctaaaactg ttcccaaaaa acctatcaag
   192781 ggagcaatag cggcaaaagt agaaagccag cccaaacctc tttccaattt atgtagttct
   192841 aaatttgctg tagcttccat actctgatta attaaatcca gatcatcact gggactatta
   192901 aacagtttat agagcattat tcccattgga cattcattct gatgtagatt aattagtgcc
   192961 cggatgtttt ccaatttatc ctgggatttc aatgattcta tcagtttatt attagcaatt
   193021 cttacttttc tgaattgacg gtatttttcg ataataattc caatgctgat tacggagata
   193081 accgtaagta catacatcag gaatccgcct tttacgatta aatctaaaat tgacattaaa
   193141 cctcacgcat gcgtttttgc ttttatatta cttttactgc tgtagtaccg gttaaggtac
   193201 tacagcagta gcattactat tatagttaat aattacccat tctgtttttg gaagttcttc
   193261 ataaattcag ctaaatcgta agctgcgttc agcggaagag cgttataaca ggaagcacgg
   193321 catccaccta catcacgatg acctttcagg cctatcattc catttttctt cgcttcactg
   193381 ataaagagag cttccagttc ttcattggga aggcggaagg tgatattcat taaagaacga
   193441 tattcaggaa tcacagttcc tctataaaaa ccgtttgaag catcaatagc atcgtaaatg
   193501 tattttgcct ttgccttgtt attctgctcg atcatggcta atccaccata gttttcaatc
   193561 catttcatca cgagaccaac catataaatt gtgaaacaag gaggagtatt atacatactt
   193621 ccatttttag catgtatttg ataatccagc atcgaaggaa gattagcggc agctgtagct
   193681 agaaaatctt ttttaaccaa aacaacagca caacctgcag gaccaacatt tttctgagca
   193741 ccggcatata ttaagctgta tttattaata tcaatcggtt tgctcataaa gtttgagctc
   193801 atatcagcaa tcaaaggaat accttcagga acatcgggat ctgttttcca ctctgtaccg
   193861 taaatagtat tattagttgt aatatgcaag taagcagggt tatctgatag ctgatagttt
   193921 tttggaatgt agctaaaact tttatcttcg gaacttgcag caatatgcaa aggtttattc
   193981 agttttttta cttctgccat tgctttctta ctccatacac cagtatgaat ataattggcc
   194041 actttccctt ccggaatgaa gtttaaggga accatcaaga attgtaaact ggctcctcca
   194101 tgcagaaaaa ggacatccca atcatcacca agaccgtaaa tcctctttgc ggcatttcgt
   194161 gcttcgttaa taattgcttc atactcttta ctgcgatggc tcatttccat aacggaaagc
   194221 cccatccctt tgtaattgaa aaggtctgac tgtgcctctc taagaacttc ttcaggtaag
   194281 acagcaggac ctgcattaaa gttatgtact cgttccattt gtatcctcca ggatattttt
   194341 tttctttcta tggacaagtt tttctaatac cttactttaa ggcaagttat tttttacaaa
   194401 tagagaaaat tattgactta aaccagcctt tatattctat gtaacaaagg tttaaaatta
   194461 tgatacatga gttattacac aataaaaaag ttatccttgc ttctgcttct ccgcgcagaa
   194521 aagaactttt tgctttattg ggaataaatg cttatattat tcctgcagag attgcagaac
   194581 cgattaatgg agaagaaccg caagttcaag ctatgcaaaa tgctttgcgg aaagcagaaa
   194641 ttgttaaaaa taaagtggat aaagatgcag tagttatagc tgcagatact ttagtagcca
   194701 tagataatca catcttaggc aagcccttaa accaaaaaga agcaagcaga tatttgagat
   194761 tactttcagg aaacactcac agcgtttata ccggtatctg tattttctat aatgagatag
   194821 cgaacataaa ctatgagcag accttagtgc attttgccga attgactgag ttagaaatta
   194881 aatcgtatat agaaacagga gaacctatgg ataaagcagg agcttatgga attcagggtt
   194941 atggagcaca atttatcaca gcagttgatg gatgttatta taatgttatg ggttttccag
   195001 ttcgtaaatt ctatgaaatg ctgaatgcca tattagaaaa ggaagagaaa tgaacatatt
   195061 agccaacctc tatctctgga taaagggtaa atttactgaa gaaccgaaat cgctacttgt
   195121 tcataataca agtatttcag agatattgcc ttatcctgtt ccagaacaat ataaaaacat
   195181 ctctatgaaa gatttaaatg gttcttatgc ctatcctggc tttattgata cgcacacgca
   195241 cagttttgaa ggaggattat attctctggg agtggatttg tctactgcta aaaatataga
   195301 agacatcctg gctctgcttt cggcagcaaa ttccaaaaag acaaaggacg agttcatttt
   195361 tgcttggcaa atggatgaga cattattaaa agaaaggcgg tttcccactc taacagaact
   195421 aaatagtgct gttcccgaca aacccttaat tttaagaagg atagatggtc attcctgtat
   195481 gctcaattct tatgcaagaa atatggttac aggtttgaaa actaaagaag agattttgcg
   195541 aggaggggaa aatgattatg ctgtccattt ttttcataaa cgcttaaatg atgaagcaat
   195601 tttaaatgct tatcgggcag cagcaaatat tggtttacag gcaggtttta cttctctaca
   195661 tactatgatt ggtgatgcag aaaatagtat taatcactac gagctcatca gagataattt
   195721 aaataaattc cccctacgat ttattctata tcctcaatct tttaatatca aagcagcttt
   195781 ggaggctgga gctaatagaa tcggaggttg tatattagct gatggttcaa tcggttctga
   195841 aactgcagct atatcagaac catatttaaa tagtaattct tggggagttc tttatcaaag
   195901 cgatgaattc tggcaaaaat ttattacaga agcaagtcaa caccggctcc aggtagctat
   195961 acattgtatt ggtgatcgag caattcgcca aattaataat gtttacaaat cactagctag
   196021 cacagcagat ttaagacatc agcttatcca ctgtgaaata actcccgata acttaatcga
   196081 agaaattgct acttctggag ctgttcctgt tatgcaacca aattttgatt atttatgggg
   196141 aggtgaaaat ggtttttacg ctaaaaaaat agggataaag cgtagtagaa taatgaaccg
   196201 ttttgctact ttattaaaat ccggcattac tattaccggt ggttccgatt ggtatataac
   196261 atctttaaat gcagcacatt cacttagggc agcaatcaat catcacaatc ccttagagcg
   196321 tttatctact gcgcaagcaa ttgatattta tactaaaaac gctgcctggt taaatagtga
   196381 agaagatact tatgggaaaa tcaagattgg atataaggca gatttatcta tctattcaca
   196441 cccaatagaa aatgaaacaa cagagcttta ctatcttatc cgcgacgggg aaataaaata
   196501 tgcagctatc tgataatgat ttaattaaag ttgatatcaa acaggttatt aagcaggaat
   196561 tactttttca tcctgaattg caactgattg acctctataa actcgttttt caggcatata
   196621 tgggaccggg acatctaatt aacaatattg acgaagcagc aaattatatt ataacagaaa
   196681 tgctgctaca ttctgaacca tatttaccta taaaacagga tatcggtaac ggaaaaggat
   196741 tttttcgtgt aagtttagac cttctgatgc cggaaaaaga gctaaaaaaa gaacaggcaa
   196801 ttgttgaaat ataccgtaaa agctatatat tggcagatct tatgaagcaa tcagtgtacg
   196861 ataacaacca taacctcaat attactgata tttggaatgc tactgtacca cagataaaag
   196921 aactgataga ttttgatctt gctgaatgga aagaagtaga tttaattgct gaaagtaaag
   196981 caattcccag acattcccag atctataaaa cactctacaa tcccaaatat agagtcctta
   197041 aatacaattt ttatgataat atcgataaat tagtgaaata atacctggag atataataga
   197101 atgaatgaaa tccgtgtccg ttttgcacca agtccaaccg gttatttaca tattggtgga
   197161 ttgagaactg ccctttacaa ttaccttttt gcccgaaaag aaaaaggtac ctttatttta
   197221 agaatagaag atacagataa aagcagacaa gttccgggag caattgaaaa cttgattaac
   197281 actttaaatg ccttagggtt ggattacgat gaaggacctg gaagagagag caaatttgcc
   197341 ccctatattc aatccgaacg cttatcttta tataaagaac atgcacagta tttattagat
   197401 tccggttttg cctatccctg tttttgttcc ccggagacct taactaaaat gcgcgaagaa
   197461 caacaatcaa aaggtgaatt tgtaaaatat gaccgccgct gtttatcttt aactaaatcc
   197521 gaaatagaac aaaaaatagc cgcaggagaa aagtatgtta tccggttaaa aatgcctgat
   197581 aaccgcaaat ttcaattcaa tgatctgatt cggggtgaag tggaaatgga ttccaaccag
   197641 tccgacgatc aggttttact aaaaagtgac gggttcccaa cttatcatct ggcagctgtg
   197701 gtagatgatc acttaatgca aatttctcat attatcaggg gagaagaatg gctttccagc
   197761 actcccaaac atatttattt gtatgaatgt ttgggttggg aaactcctaa atgggtgcat
   197821 cttcctctta ttcttaatgc tgaccgtaca aaattgagca aaaggatgaa tgatgtatct
   197881 gtagatagct atatagcaaa aggttatctg aaagaggcac taatcaattt tgttgcttta
   197941 ctgggttggc atccttccga tgaccgcgaa cttttcactc tggatgaact ttgcaatgcc
   198001 ttttcattag aaagagtaaa taaagcggga gccatatttg atttaaccaa gctggattgg
   198061 atgaacggac aatatttaca ttcactgcct gttgaagata ttgctatgcg ttgtaaacac
   198121 ctttttttag agaagggata tgatcttagc gatgaaaata aataccaact tgctattgct
   198181 gtagctcgcg aaagatgcac tttactaaac gaaattgtgg attactgtaa aatgttttat
   198241 caacctgagg agttaaagaa tgacgatata aaaacactat tagctccgga agctcaaaaa
   198301 gttcttgctt ggtttgcaac taatttacca attttatctg cttgggatac ggaatctatt
   198361 cagaatttag ttaattctgg aattgctgaa ctgggtatta aaggtaaaaa attctacact
   198421 cctttacgtt tggctttaat tcatcaacag catgggcctg agctaccttc cacttttatc
   198481 attttaggaa aggaagaatc aattaaacgc ctgcagcaat atctgccgag ttaaattcaa
   198541 ataatgtgtt ggaggataat aagttggcag atttgttttt gccaaaaata atggagttat
   198601 ctaaatgtac tctttcccta aattgcggaa tcgaaattca gcagcccatc accatatttt
   198661 ctaatcaact aaatctaagg aattatataa gatagcagtc tccttactgg aagcttatat
   198721 acagtcaata agcatcccgt atgattccct tatctgatat gggaatcata tgggaattat
   198781 ataggctgca tacgagtttt taaaaagtga tatatacgaa gtaattatta gaagtgggta
   198841 aaaaaggtgc ttgaaatttt aatatttgta ttcacataaa taccattgtt atttatagtt
   198901 taaatagatt aatttgctgg cgagtttgga aaaaaatata ggaaaaatct acgcatactt
   198961 atactagaaa gttgcctcag catttgtatc agcgactttc ttttgtaatt agaacacata
   199021 gtatacatct tatctgcttt ttgtagatca taacaaatgc agaatattga gtataaaagg
   199081 aattattatg gctaagtatg gaagattatt aataatcgag atttcattgt tttttttggg
   199141 tgcctgccaa gaagaaagtt tgttaggtcc cttatctgaa agttttgatc ctctcagcat
   199201 ccaagaagat agaattcctc ttgccggtgg tgaatatctt taccaacaaa acatcgtaat
   199261 taccaatacc gaggataacg acttttttgc ttataagtta ataacttatc aaggtgaact
   199321 tccggtatct tatatgaatc aaacggaaac agcaaacgct ttacagaatt tatttagcaa
   199381 tatgatcaat aatgaacctt tataaggagg ataaaatgaa gcactatata atcttgatcc
   199441 tacttctttc cattgtcctt ttgtttggtc aaaccaaacc ttcagatgca aaaagtgtaa
   199501 attatgaaac ccataattcc ggtttacttt cacttaatgt ttataatatt ggatctctct
   199561 caaatttgca taatccttat gatggaatga agttaatgtc tcaaagcgga cagtggattt
   199621 ctgccaaaaa atatcgcaga aataatctaa atcaattatt gtattggctt tctcccatac
   199681 ccggtcctgg aaatgatcag atggttacgg agatggatcc tctttggaat ccaaatttgt
   199741 tggtagttac agatacactt tccacagttg cttatgatgg cgattgctga ttagggcttt
   199801 tttttaatca ataatatgat gcattataga ttctgttgaa gttcccagat tctatctctg
   199861 agttcagcag catgttcaaa ttcgagattg gcagcagcct tattcatttc tttagtcagc
   199921 agttcaataa tcttttcttt attatcgaga tctaaatatt ccaaaaaatc cttttctgaa
   199981 ggtttttcat cggtaattgg ttttgatatc ttatcatatc cttcagcaat agctgtcgat
   200041 tgcataattt gttcaattgt cttataaatt gtttgtgggg taatattatg ggctttatta
   200101 taggcaagct gttttacccg gcgtctatta gtttcggtta tagtttgttt tatagcgtct
   200161 gtaatttcgt cagcatagaa aattactttg ccatcaacat tacgagcagc tcgacccgaa
   200221 atctgaatca aggagcgagt tgaacgcaaa aatccagttt tatcagcatc taaaatagct
   200281 actaacgaga cttcgggtaa atctaaacct tctcttagaa gattaactcc aacaataaca
   200341 tcgaatactc ctaagcgaaa atctctaata attttagcgc gttcgatgct gtcgatatcg
   200401 ctgtgcatat atcttgcttt gataccagca ttaataagat aggtactaag gtcttcactc
   200461 attcttttag tgagagccat caccagaact ttttggcttt taactgtgcg ttgtttaatt
   200521 tggccaataa gatcatcaac ctggtgcttt acaggtttaa tttcaatttc tggatcgagt
   200581 aaaccagtgg gacggataac ttgttcaaca atttcgcctt gagttaattc caactcataa
   200641 tttgcaggag tggcagaaac aaaaatgaca ttacgcatat aattttcaaa ttcttcaaaa
   200701 cgtaacgggc gattatcaaa tgcagaaggt aaacggaaac catactcaac caagtttttt
   200761 ttgcgagtat aatcacctcc gaacatacca tgaacctgcg ggattgttac atgggattca
   200821 tcaattacaa agagaaaatc ttcggggaag taatccagta aacagtttgg tggttcacca
   200881 ggtttggcac ctgttagatg gcgggtataa ttttcgatac cactacaata acccagttca
   200941 cgaagcattt ccaaatcgaa agcagttctt tgttttagac gttctgcctc caggtatttt
   201001 tgattttgca taaaccaggc gatgcgttca ttcatttcat tttctatatt gatgatggct
   201061 ttatgcaatt tatcctcgtt cattaaaaaa tgaattgcgg ggtaaacatc gtaattttca
   201121 acttctccca aactgttaca ggagatagga tgcagttttt ccaaacgata aatttcgtct
   201181 ccaaaaaatt ccacgcgtag acagtgttcc aaataagcag gaaaaatatc tacagtatct
   201241 cctctaactc tgaatgcacc tctttcaaaa gcaatatcat tgcggctgta gtgagctgca
   201301 accaattttt taatcaattc ttcgcggtcc attttcattc cccgcgatat agaaattaat
   201361 gcttcacgat attcttctgg aacaccaaga ccatagatgc aagacacaga ggaaacaatt
   201421 attacatccc ttctttccat taaactcatt gttgctctta accgtaattt ctctatctgt
   201481 tcgttgatac tgctatcttt ttcaatatag atatcttttc cgggaatata tgcttcaggt
   201541 tgataataat cgtaatagct gatgaaatat tcaactgcat tttcggggaa aagttgtttt
   201601 aattcaccat aaagctgagc agcaagagtt ttattatgtg ataaaacaag ggtaggacgg
   201661 tttatctgtt caataacatt ggcaattgta aatgtttttc cgctacctgt aacgcctaaa
   201721 aggacttgat attttttatc ttctttaatt ccttttacaa gagattttat cgcttcgggt
   201781 tgatcaccgg aagggatata atcgctaaca agtttaaagc gttgcattat ttgtctcctt
   201841 tttagttaga aattcttctt gacattacag ggcttgaatt ctgaattgct ttacaaggaa
   201901 tatgaatccc ttgttttgtc cagaattatt tagcttggag atagaaaaat gcttaattac
   201961 gaagatttac aaattaacta cgaaaacctg ctcaaattgg gttatgctgt cgttgatgtc
   202021 cgttataagg actacgatgt ttgccctgat attcccaaac tggttattgc cagagttgat
   202081 tcagacaggg atgattttta tcaggatatg ctgaaactct atactggaat tgaatttaag
   202141 cccaatgaaa tgtttgagat ctggacagat atcctcagcc ataaaatcaa aatgagtata
   202201 gttttacacc gtgatattgc cataaaagtt gccgctctgg attatatcga aactgtatat
   202261 ccctcaaaat gaaggaacaa tcactactgc taattaaacc aaatgcagtt cgcaacaagc
   202321 atatagggca tattgtaacg attttggaag agaatgattt ccatttttgt gcccttaaaa
   202381 tattccgttt cgataggcag ttgggggaac ttttttatgc tgaacataag ggtagagcat
   202441 tctttgaaaa tttactgagc tttatgatat cggatgacac tgtagctatt attgtcgaaa
   202501 aagacaacgc tgttcaggaa ttaagggaaa taatcggaga tgtagatccg gaaaaaagaa
   202561 aaccgggaac aattagagct ctttatgccg aagggattac tgaaaacgca gtacacgctt
   202621 cggactcaat tacaagtgct attagagaaa tcggaatcat atttgataag caaactaatt
   202681 agcacagtaa aatccttcag caagatttga aaataaggcg aataataaca aagagatatt
   202741 tctgagccct gttttccact ggatataaaa taattggtga aattacacta tgtgttttcc
   202801 tacttgatta tatactgcaa agatatcgtg gcttcactgg gtaaaatgcc aagataaatt
   202861 atcgtatcta taccgcaaat aacaaggcaa gaatttgaac gggaaaagag taggggaaca
   202921 cttattattc cggaggatgg ttatccagtc gttctttaaa gagatataaa acaggagaaa
   202981 actgggatat atattttttg gctaaagaaa gtactttgcg ggcttcttta taattatttt
   203041 tttgcaggag cagacggatt aactcttcat aaactacact tatataagga tttatttcct
   203101 gagcttgtct taatatttca attgctcgat caatcagacc aatgttggtg gctgcattag
   203161 cataggcaat taggtgatcc ggatttgtga tatggcctaa agactcatat tctacaaagc
   203221 tgtggtagga tttcaaccaa atgccttttt tgccataaac taaagcagaa agtagcaaaa
   203281 gctgtctatg cttgccgaat tttttttctg cattaataat tgtttgatat gcttgatcgt
   203341 agttttgcat tctgacatac tgttcaatca ggagaaaata gataaatgga ttatcatcca
   203401 taacttcaag gattttattt attacatcga tactttcctg ataacaacct aaaaatgcat
   203461 aagctaatgc actattatat agaacttcgg gaggtggatt actaatcctg ttgaaatagc
   203521 tgattgcgat tctatattct cctaaggaga aataacaagt cccaattaaa aaaagcaact
   203581 gggggctatc tggatgcaag gaaatggctt taatataata actaagggct ttttcatata
   203641 tttgcctttc gagatagata tctcccaaat tgatgataag ggaataatta tccggttctt
   203701 ctttcaaacc ctcttgaagt aaataaactg cttgtagcca attagtttta gccaggacag
   203761 ttgcccgttg aatgagattg tttatatcag ataaattcat actatttaag ctcttttaga
   203821 agttctttaa cagcacgcac aagttgagga tcacgctctg caataatatc ctcaggtaaa
   203881 tttaatataa ttatatcagg cataactcct gttccttcca tattggtacc atcaagctta
   203941 taccaacctg ttcccggcat tctcattgaa gaaccatcct gaagataata atgccaggtt
   204001 ccaataactg ctccactaga aggagttcca actaccttgc ctaattttaa ttcttgatat
   204061 accataggga aaatttctcc atcgctgaaa gattcctcat taactaatac aatgcttggt
   204121 ttatcccaaa tacttcgagg ttcggaacgc aaaattctac tatacctacg cgtagcggta
   204181 tacgcatact tctttttttg cagtagtgtg ataatctgat catgaatatg gcctcctgaa
   204241 ttaccacgaa catctataac caaagcatcc ttatcataat tatccactaa gaactcgcga
   204301 taaaactgtt tccaatcatc atttcccata gctggaatat gaatataacc taatttgccg
   204361 tgagaaagat catctacaag tttacgattt tgagctatct gataattata ccgcagctcg
   204421 tacatctggg aataagacaa accggtaata acagcttcta tatcaactcc atcttgaaca
   204481 actgtaagct ttatcttctt cccaattttt cctgcaagca gtgaatccaa cgatgtcttt
   204541 gaagtgattt tcactccatc cacattagta agaatacagc catctttcag atgataaaaa
   204601 ttgcttaaac gggaatttgg ataaactcgg cttaagacaa ttccttcttc cagaattata
   204661 ttataatcaa attcggctcc cagccaagcg acaggattcc tgaaaggact atattcgctg
   204721 cgtggataaa atccagtatg tgaagcattt acatcaccta tcatttcttc tattatagtg
   204781 gctacatcat aaatatctct tgccttatct acataaggac ggtataaacg gaagagttct
   204841 ttccaggaaa cattatgcat atccggatcg taaaaattat taccgaaagc actccatgct
   204901 tcttcaaaaa ctcgctgatt caaaagggct ttatcatact gataatcata attaaccttg
   204961 atatctttgc gagaggaagt agccaaatta taggacttaa gttcaccctg tacaagataa
   205021 tagacggtgc tatttacttt acagtaagca ttagctgtgc tgccaaaatt gaatacttcc
   205081 ttttgatttt tgccgtagat attagctttt ttgaggtagt tatttttatt cggttgattg
   205141 gcatcttcaa catagtaaaa agtactgtca ctaatcgtgt ccagcacgaa attataatta
   205201 tcctttgcta ttataatagg gtaaaagcgt ttttcgatac cttcccaaac aatttccagt
   205261 ttattatttg tttcagtttt attctcttct gctacaatat ctaaaactag atagccattt
   205321 tcgtcatagt acatttcttc atttgtgcta tccggtttac ttttttcttg gttcaaaatc
   205381 tcttcccagg gatctttatc caattcaaat tcatctctcg gaacaagatc caggcgatat
   205441 atatcctggt ttcgggttaa cagtatagac ctattatctg gcatccagac aagattatcc
   205501 agccaaccgt catccgctaa aattcgttta ctggtatctg ctttaacatc gtagagatat
   205561 agttctttca cataattatc atcgcgcata caaccataca atgcataatt gccatcctcg
   205621 ctgaaagcaa aattggtggt aacagcccaa ggtgttttta ctggtcgaat attaatcaat
   205681 cctgaatcgg caatagctac tttttgatgg ctgtaataat ctgcatattt gaaataccaa
   205741 cgaccatttt tgtctttacc aaggtctgaa acacataaac tatccgctcc aaaccactca
   205801 ataggggaaa gggttataat ggaatctgct gttgctacaa acagttttac aattccatta
   205861 tccagtttgc taattaccat tcggcggtta tccaaaaatt gcatactgga aatggaaccg
   205921 tgatcataac taatctgttt tacctcccca cctttcactg gagcaaaaaa acaatcgtaa
   205981 ttatgagtaa aaccaaccag cagttcattg atagaaacag tataatcacc tacattatta
   206041 ttgctatagc ttctaacaat attgtcttgc cattcgtctt cagaaagatc tatataaaga
   206101 gggctaacct tctctccggt ttttatagtg ggatcatatt tatagatagt gtcaaaatat
   206161 tcaaagacaa gacgctcatt atctcttgcc agatttatat ctcgggcact gaaagggcta
   206221 agattagtaa ttttttcagg tttacgaaaa gagagcttgt taaccttgtt aatctgtaaa
   206281 tttttaccat ccgatgcaca ataatataaa gctcctgttt gtgaaaaacg gggatacatt
   206341 tctgagagct cagtattagt aagttttgta tattttttcg tttcaatatt aagtagccat
   206401 agttctccat ttgcactgcc tcgatatgct tcccgccttg aataaccacc cttttggaaa
   206461 acaatttgtt tattatcagg acttaaggta gcaaaacgag tagcaacttc tgctatcata
   206521 acaggtctgg aaccatctat aggaacttta tagaaagata ttccccatct gggagtgtat
   206581 tttgtgcata aaagatgctc ggaatcacta aaccactcag aaagacctaa gctctcatag
   206641 gttacaggtt ttgatattcc cccttcggta ggcatcaaat aaatcatact ttgtccatca
   206701 cgatcagaag aaaaagcgat cattttacca tcggggctaa attgaggatt ccattcttta
   206761 caagtagtat tagtaattct acggggaatt cctccttcaa aaggaactaa ccataggtca
   206821 tcacaataaa caaaacaaac agttttgcca tcaggagaaa tagcaggatc acctgcaaac
   206881 ggtgtctcaa gagctattag agtataacta aatgataata acattactat cgagaagatt
   206941 ctttttaaag tattcttaat tgttttcatt attccttttc tataaattct attttctttt
   207001 ctcgtttgtt aatttcgtag ctatgtttta aaatccgata aataatgggg ggtctggaaa
   207061 tatcctgaat agaaattctc ttggtatctt tctgctctct gtttaggaat tgattcagct
   207121 tatcgtccaa caatatttca tagttttgga tgcggttttc taagtccact atagctgtag
   207181 catttatatc tttattctct tttagacgaa taaggtcttt agttaagatt agcaacaaat
   207241 tgcgataaaa aggacttata gctatctcaa tctcggttat tgtattatta taagaacccg
   207301 cttgaaaagc agtaattgat tctcctgctt gagttaaacc accgcaaatt tcacttctat
   207361 ctggtgcacc tattatttca ccatcacata ctactgtcga attttcaagt ttattggtaa
   207421 aatggatatt attgaggcac actatatagg agttatggat actgctacat tccaaatttc
   207481 cccaaacaat tatgccctta gctgaagaag catttatatc acctgtaacc agcatatcct
   207541 ttttacattc aatactgcag gaatgtactt ccccctcaat tctcaagttt gcatcacaat
   207601 gcagattagc ataagtaaga tctccttcga ttattaaatc aattggacaa cttataattg
   207661 tgtcacttgg aagttcgctt gcttttagag taatgctatc taaaagactg atatgaccct
   207721 cctcgtctac ataaggatag ccatctttta gaagataata agtacagttt tgaacatcat
   207781 aggcaacatt ttctccagcc aaggcaattg cctgttcagg atcaactgaa ctagcatcta
   207841 tcaattctcc aaaaatatta taaatgctcc ccttctgttc aaatatatta tgtgaatatt
   207901 ctgctacagt atctccagct ttgaaaacct tcaatttggc taatgtttga tagctgatct
   207961 ccatttcagg agcagttagt atatcgggat taaatttata gcgtaggatt gttttattct
   208021 catccttttt acacatagca accgggaaag gaatatcaaa ttctttttcc agagaatgaa
   208081 tctgtgcata ttcaactgct tcttcaaatc cgcttttgat acctgcttct tcaagtaaag
   208141 caataatagc gtattcatcg ataagcttac ctgttttttt tatggttaac caagcagaca
   208201 agggctcatc acgaatttct aaaatcaggt taccttcttt gttggtgtag gtcttatcca
   208261 tgtatgatac ctacaatgtt tatttgacga ttattcgttt tttcccggcg gtaagaatga
   208321 tagcaagagt tttcaaaagt gcaatcattg atgtgttcaa tattgatttc cggaattcca
   208381 gcttttaata gctgagcatt aagggttttg cgtaaatcga tatgccggtt cttggttaaa
   208441 acaggttcca atccctgaga aacaatactc ttaccaaact cgttataaag ttcttggcta
   208501 acttcataat gattatgaca gatacctgca cctacgatag ctataatatc agtaagctgg
   208561 caatacattt gactattcat tattctgatt gcttctccaa ctatgttttt tctggttcct
   208621 tccctaccgc aatgaagtgc tgctataaca tttatacggt tatccattaa aataacagga
   208681 taacaatctg cagttctaat aagtaaatat tgatagggta ttttggttat taatccatca
   208741 actacaggaa tttgaggctt gttcataaaa cctgcaccac catcgatttc tctacaaata
   208801 tggacttgtt tggaatgggt ttgttcagca attattactc ttgaaatagg gatttttgtc
   208861 ccttccaaga taaaatcctt ctgtaaatgc atcaaagatc ggtaatccgt atctctctta
   208921 cctagatggt agaaaatctt cattggatat taatctccgg agcaagaatt ttggaacgat
   208981 aaacatctgt attattgctg attactagca ttcttaatac tcccttaata aactttggag
   209041 ctaactttaa gctatacaat gaccacaata aaaatactga agcacaaata attaaagtat
   209101 atcgagctcc aaactgactg gataaactac ccatcagtat actccccaaa ggagctgtac
   209161 tggctgaagc catagtatat agtgcaatca ctcttcctct catttctata gaagcaatac
   209221 tctgaataag agtatttgtg gttgccatag tcatcattcc tgttaaacca ataaaaacca
   209281 tatagaacat actaagagga agcgaaccgg atagagaaaa taaaattaga gagatactat
   209341 atactaaaca aagatataca agacgagttg gcatccctct tatatttttg cgcgaagcta
   209401 agaagaaaga accagctaat gctcctactc ccatagccga cattaataaa ccctgagtag
   209461 cagaagtgcc ctgtaaaata tccttagcga aaataggcat taaagtagaa aaagacattc
   209521 caaaaattgt atatattgcc aaattgctta ttaaaaaccg aatgggcata ttctcccacg
   209581 catatttcca accagaaact atttttttga gagtaggttc tttttgagga gaaggtgggg
   209641 gatagcttat tctaataaag cacaaggaaa ttattactgg aatatatgaa atagcattaa
   209701 tagcaaaaca taccccttca ctaaacatca taattagaaa accaccaatt gcaggaccaa
   209761 ttaaacgcgc tccattaaac atagcagaat tagttgctat cgcattgggc aataaccgtc
   209821 tggaagaaac aagatccatt acgaaatttt gacgaatagg ggcatctata gcatcaataa
   209881 ttccttgaag caaagataat attataatag gatactgaac ctgcttgttg attactccgg
   209941 tcaaaactaa tatagccaga atccctgttt gtaagcagaa agcaatttga gtgtatatca
   210001 ttatatgatg gcggtttaca cgatctgccc aagcaccagc aaaaggactg acaaaaacag
   210061 atggcaccat tgaaagaaaa ctcactaaac caagcaaaaa tgcagaaccg gttaaacgat
   210121 atacaaacca gcccatcgta gttcgttgaa tccaggttcc aattaaggaa atcccctgtc
   210181 ccataaaaaa gagacgatag tttctaatag ttaatgaaac gaagatttct tttagttttg
   210241 aaatgatcat tgctatagga taaagcggga aaggtcctca ctttcgatga cagggttaag
   210301 atattcagtt accatctttt ttgtgatttt aacgctttta agagtttcgg aaggcatttc
   210361 aaatagaaaa tcatcgagca gtgtattcat tacagtatgt aaacgacgag ctccgatatc
   210421 ctccattttt tcatttgcta aagctgcaaa acgagcaatt tctcgaattg cacttagcat
   210481 aaatgttagc tcgactcctt cgcttcggaa catctcttga tactgttttg ttaatgaatt
   210541 ttctggctca ataagaatgc gttcaaaatc ttccttagtt aaactattga gttccactct
   210601 tataggaaat ctgccttgta attccggaat taaatctgaa ggtttagctg aatgaaaagc
   210661 acctgcagca ataaatagaa tatgagaagt atcaatcatt ccatatttag taggaacttt
   210721 actaccctca acaatgggaa gaagatctcg ttgtactcca ctacgagaaa catctaatcc
   210781 accttgctta tcaatattgg caattttatc aatttcgtct ataaagataa tcccattttc
   210841 ttccactgaa ttttttgctg tttcaattag tttatcttta cttatcaacc ggttaatttc
   210901 ggtttcaata tatcgtttcc aggcagtgtg aacagtggtt ttaattgttc tggatgcacc
   210961 tttaccctga aagatattag tcaacatttc ggtaaaatca agatctaata attctaaacc
   211021 tggtcctggt aatatttcca gattgggagt agtgtctaat tcgggttcaa tttcaatttc
   211081 tttatcatcc aaactacctg ataacagctt tttacgcatt ttttcccgac tgcgtaaata
   211141 cctatcatat tctgcattat cactaacgcg attttgtttc atttttttgc tgcggggaag
   211201 caaaatatcc aggatcattt cagttgcatt cttttctgct tcaggacgaa cttcttctat
   211261 catcatctcg cgaatctggc taactgcata attcattagt tcacggacca tcgattctac
   211321 atcgcgtcca acataaccaa cctcagtaaa tttggatgct tctactttga caaaaggagc
   211381 tcctgtcaat cgtgaaattc ttcgagcaat ttcagttttt cctactccag tagggcctat
   211441 cagtataatg ttattgggaa taatctcccc cttgatatca ccagatattt gttggcgacg
   211501 ccaacgattt ctaagagcaa tagctacaca acgttttgcc tgatcctgcc ctattatata
   211561 tttatccagt tcttcaacaa tccgttttgg ggttaaatgt acatagttta acaatttcta
   211621 aacttcctct acaatatagt tactatttgt ataaatgcat atttcagaag caatatttat
   211681 agcttcaaca gcaatttcac cagcacctaa ttttgttttt ctagctaaag cgcgagcagc
   211741 tgccaaagca taatttccgc cactaccaat agctattata ttatcatcag gttcaagcac
   211801 atccccaact ccattaatta gaagaagggt atctttatct ccagcaatta gcattgcttc
   211861 caaccgtctt aatattctat cttgtcgcca gtccctggct aagtcaattg ctgctttgcg
   211921 taaattacct ttattggatt ttaatttatc ctcaaatctt tccaataaac tgaatgcatc
   211981 agctgtagct cccgcaaagc caacaatcac ttttccatca aaaattcttc gtattttttg
   212041 ggcattgcct tttacaatag cttcacccat agtaacttgt ccgtctccgc atatagcagt
   212101 tttaccgttg cggcggatac ctataatagt agttccatgc ataatcataa ttatgaagtt
   212161 tcctcaagtt ttgtatctgt gtcttcggtt ttatcttcta atgtttcagg ttcagaaact
   212221 ctagctttct cgccaggttc aaagcgaagt tcaagagctt ttttatattt gttttgtaca
   212281 atttcagatt catcatcgcg gcaattatca agaataagct taaatatttc atcagtgcct
   212341 aaagtttctt tatcaaaaag ttcattagcc ataatttcca tcagttcttt atgctcattc
   212401 aagattgaaa tagcttgaga ataggcatta ttgatgattt ctctaatttc acgatcaacc
   212461 atttgtgagg tttcatcact atatatatca cgggaaacaa gttcctttcc cagaaacact
   212521 tctccctgtt cttttccaat agtgatagga ccaattttat cactcattcc ccaagaacag
   212581 accatttttt ttgctatatc tgtgctgcgt tccaaatcat ttccagaacc agtagtaaat
   212641 tctgtaaaaa taacttcttc agctgctctt ccacctaaca gggtaactaa taattgaagt
   212701 aaatagctct tggaataatt ggttttatcg cttaaaagat aatgagttgc tccaccagta
   212761 aaacctctcg gaataatgga gaccttatgc acagcttcaa ctttatccaa gaatacagag
   212821 gtgagcacat ggccaatttc atgataagcg gtaaggcgtt tgtcttcttc aggtataact
   212881 ctgcttttct tttctttacc tagaataagc ttatctttgg cttcttcaaa atcatccatt
   212941 tgaatctgag tcttgttttt gctggcagca atcaaagcgg cttcattaac cagattagct
   213001 aaatctgctc cactaaatcc aggagttcct cttgaaatta tctccaagtg tacatcatta
   213061 gcaagcggaa ctttagcagt atgaactttc aagatttccg tacgcccttt aatatcaggt
   213121 aaatcgactg taactcttcg atcaaatctt ccaggacgca gtaaagcagg atcaagaata
   213181 tcaggacgat tagtggcagc aataataatt actgcttcat tcggctcaaa accatccatc
   213241 tctactaaaa gttgattcaa ggtttgttca cgttcatcat gaccacctcc taaaccactt
   213301 ccgcgatgtc taccaactgc atctatttca tcgataaaag taatacaagg agcatttttc
   213361 tttgcttgtt caaacaggtc acgaactctt gcagcaccga caccaacaaa catttctaca
   213421 aaatccgaac cactgattga aaagaaggga actccagctt ccccagatac agcttttgcc
   213481 aaaagagtct ttcctgttcc aggccgtccg ataagcaaaa ctccccgcgg aattcttcct
   213541 cctaatcgtt gaaacttttt aggatccttc aaaaattcta caatttcctg caattcttct
   213601 ttagcttcat caactccggc aacatcttta aaagtaactc ccgttttact cgcttcgtat
   213661 aatctggctt tacttttccc aaaactgaat gccttagcat tttgagcatt catcccacgc
   213721 aagaaaaatg cccagaaaat tattaacaat aaaaacggta atagatagga tattatactt
   213781 gcccaacgcg atggttttgt ggcgctgact ttaattccca ttgctacaag agaatctaca
   213841 agctggggat tttcgaaagg cagggtagtg gtatatttct tattggcaat atcagtgtaa
   213901 aaaatgtcct tttctgaaaa ctgtacctgc gttatttggc cattagcaac gcgtgccata
   213961 aaattagtat agctctcttt agttatagaa gtaccgcttg tactaaatat atgaaagatg
   214021 attattacca taaggatgag cataataatc ataggtaaag agaaagcaga ttttttggga
   214081 ggtacatttt tggggggttg aggatttcga aaatccggat tcggtttatc tttgttctta
   214141 atatttcgat ttctaacaat tatggatctg ataatactga ttatcgaaat taataataaa
   214201 gccagaataa accagttcat aagtgtggaa aattgcataa aagcatttcc tgcctcaggg
   214261 agagttttct gaggcacagt taatgtgtct gcagttgtag catttaaaaa acctaatgct
   214321 attatgcaga atagaataag tatgtatttc atcatttata tagctcttct tttaatattc
   214381 ctatataggg taagttacgg tatctctctg caaaatcaag tccgtagcca accacaaatt
   214441 cgtttttaat ggaaaaaccg atgtattcaa aacatacatc tattttatgg gcatcgggtt
   214501 tatctaaaag tacacaggtt ttcaaactgg caggtttatg ttgccagata taatccttga
   214561 tatattctaa agaaagaccc gtatctacaa tatcttcaac tataattaca tctctgttag
   214621 acaaatcaat atcgatatcc ttgcgaattt ttacgattcc actactatta gcatcattac
   214681 catagctgga aatagctaaa aaatcaactt ctacagggat agtaatactc cggactaaat
   214741 cagccaaaaa gataaatccc ccttttaaaa tacctaccat aacgggtgta ctatctttat
   214801 agctgttgga tatttccaag ccgagttcac gaattcgagt ttgaattttg tgttcgtcaa
   214861 agagaacagc agaaatatca caattcatct tattcatcct ttcctctttt cttttttatg
   214921 gattgccctt ctggattttg aagcaatagg gtttgccact atttgcaaat agcgagaagt
   214981 ggtttcatca cattttaccc gatcatccaa acggcatcct acaatccaaa ataacttttc
   215041 tccatcttct aaaatgggta ttaaatccct ctcatattta gggacttttt cgtcaataaa
   215101 aaactcttta agacttttat attgtgtcat cccaaaaggc ataaaccgat caccgggttt
   215161 tctgaatctg atcataaaag ggaaattcac tttatcggca tcaataataa tttgttcttg
   215221 accatctgcc agcttatgat ctttgggttg taccttcaaa tatttgaaac tgaaacgata
   215281 attgccataa acagccatag tacggtctgt atctatgatc ataggttctg gttctgtatt
   215341 ttttttaata tctccaactt ggataattaa ctcctcatat tgttttataa ccacgatatt
   215401 tttgggaaga tagatacatt tactgccttt agtagcaaga atattatcta tcgcctgata
   215461 gtgaactgca aagaaatcat tatcatcatc gcagaccttg ataaagacat tgcgaagtaa
   215521 ataatacctt tcaattttgc taagctttat aagtagtggg caagataaaa ctgttctttc
   215581 gggttgtata tctaaacaaa ttcgtttata ttgagactca ctgcgctgtt ttaaaatttt
   215641 atctgcctga gcaaaaagtt cggctacaaa tgagagatgt gaagataaca taggattaaa
   215701 ttctctttca agacgaggaa taagttcatt gcgtaaaaga tttctacgga atttattgtc
   215761 caaattactg gaatcggtcc tccaggtaat atccacttta gataaaatat cctcaagttc
   215821 ttttctacta aaacaaagca ttggatgaac aattttacca gaaatagctt taatccctgc
   215881 cataccagta attcctgaac cacgaaaaag atttagtaaa actgtttctg cttgatcttc
   215941 tctttgatga gcaagcagaa ttttatcaaa tttataacta tccagcactt tcgcaaaaac
   216001 tgaaaaccgc agtttacgag catggttttc caaatttcct gttttaggaa cttttacttt
   216061 atgaataatt aaggggatat tcaaattgga acatagttct tttacagatt cctgatcagc
   216121 atcactttca gctccgcgaa tctgatgatt gacatgcact gctaaaagag ttaaattata
   216181 agtaactctc agctgagaaa aaagatagag catagcatta gaatctgcac cacctgaaca
   216241 tccaagtaac aacttatcac ctatcttaat aagctcattt tcattgatgt ttttttccag
   216301 acgctgaagg gcttgggcaa tttccatctt gattcctcat ctcataatat tcctattttc
   216361 tattattttg atataggttt gagtgtcaat aggttttttt acagaaaatt gcttattcgt
   216421 ttaacaatta taattgtata aattacgagg tttatatgct atgattgatg aacctatagt
   216481 gaatacaggc aaatcagtta tactaaatta tacttgcagt gggttcttgc tatgaatcct
   216541 ttaagaataa cattattgta gggttttgtg tgtttattgc tgataccaaa catactattt
   216601 ccttctgtgc ttgatttcgt atattttgga agctatatta aagattcaat acttgtttta
   216661 aaattctctt atttcatcaa ggaaatgcta gaggatccct aaagaatctc ccaagaagat
   216721 atcagttaca taacaataaa atcagattat tcaacgggct tattataggg gatttcctat
   216781 tcattattta atgtctaatg gtttccgaca atcttattga tagttggatt attggttaaa
   216841 aacgagtaaa aaaacttgac gaataatcaa ataaacacga aaaggaatta taattaatta
   216901 attgagcgag gaaacgatgc aggcagacct aattattatt aatattagca agttggttac
   216961 tcttgcccat aatggccgcc cccgtttggg aaaagaaatg gaagagacag ctacaatttt
   217021 aaataaagca ctggctgtaa aagatggtaa aatcttagaa ataaacgata gtaagtcaat
   217081 tgaagaaaaa tatagaaatg tagaatgtat tgatgcaaat ggacaaatag ctacacctgg
   217141 gttcgttgat tgccacactc atcccatttt tgttcataca cgagaagatg agtttgcaat
   217201 gcgtatacag gggaagactt atgttgaaat ttctaaggct ggaggtggca ttctgaatac
   217261 tattcagaca acgcgtgatg ctgatgagga tttacttttt gaactagcca aaaggcgtat
   217321 tttaaaaatg atccaacaag gtactactac tctagaagca aaaagtggtt atgggttgga
   217381 tactgaaaat gaactaaagc aattaagagt aattagacgc ttacaggaag aacttctgat
   217441 tgatattgtg cccactttta tgggagctca tgaatttcct cctgaatata gaaataatca
   217501 ttctggttat gtagaactac tttgtaaaga aatgattcct gctgttgcgg aacagggaat
   217561 agctgaattt tgtgatgttt ttaccgaagc acatacattt aatattgaag aatcgcgtaa
   217621 aatacttagt tgtgctgctg aatatggatt aaaactaaaa atgcatagtg atgaaattga
   217681 atcaattggc ggtgcagaac ttgctagtga aatgaaatgt atatctgctg atcatttagg
   217741 ttcagctagt gaaacaggaa ttaaggctct acaaaaagcc ggtgtagtac cagttttgtt
   217801 acctgccact ttattttcct tacagagtaa aaaatacgcc aatgcgaaat tgatgatgga
   217861 attaaatctt cctgttgcta tagctactga ttttaatccc ggtagctgta attgtgatag
   217921 tatgtcctta acaatgtctc ttgcctgttt acaaatgggg atgactcctg ccgcgtcttt
   217981 atgtgcagca actttaaatg ctgcttttgc tatagataga ggcaaaataa ttggctctct
   218041 ggaaactggt aaacaggcag atatagtact ttgggatatt cccgatttga attttatccc
   218101 ttatcattta ggatcttctt atataaccag cgtgctaaaa aatgggaaaa tagtgtacaa
   218161 ggcaacctaa atggctgata catctctaaa tagctatcaa ataattaaaa ctgtaaacag
   218221 ctctttgcgc ttttctctct atcaggcaaa acagatagaa acaggaaatg tagtcctaat
   218281 taaaactcca gatagccaaa gaacaaatga cataggatta aaacacgatc tagttaatga
   218341 agctaatagc catttaaaac taaatcatcc tttcatcaga actgtttatg aaacaagaga
   218401 agaaaaagga actgcctatc taattggcga gtatattgaa ggaagatctc tctctgctta
   218461 tctgaaaaat aaccctaatt catttacttt ggaagaatca ctttctcggt tgcaagaact
   218521 tttggaagcc atctcttacg ctcacaaaat gggttgtatt catttgaatt taaatccgta
   218581 taatatcctt gttgataagg aaaaccatct gcatattatt ggtttcggta aaaaccaaga
   218641 tgcttataaa accgcttacg aaaaagatga gacctatcat ccactattgt acattgcacc
   218701 tgaactgtat tcggcgggaa ttgcccttcc tcaatccgat ttatattctt ttgctgtaat
   218761 tgcttatcat atactatgtg gtgtcattcc ctggcgggta gacaatcaac ttattcctga
   218821 aaaacaaaaa cagcagtcac tttgccgagc tgttattatg ccagagattt taaataaaca
   218881 agttccggat tggctttttg cagctttgtt gcaatgtttg aaacttgatc ctcatctgcg
   218941 gttttcaaat gcagaagaac ttttggaact ttttactaaa agggataatt gggttcccga
   219001 acaggaagca gagataatta aagaagtaga aaaggttgaa accattgtag acatagagaa
   219061 gtgcgaagcg attgagaaga tagaacaaat tgaagatgtt caattagttg atgatttgcc
   219121 acctacaaat ttggcattaa atgtggattc acagcaaaaa attaatacag atatctcaca
   219181 tctctatgga aaattggatg ttactatccc agaagaaata cctcaatccg attcagtaaa
   219241 aacaatatcg gatactactc ccaaagatac tcctaaatat tctttcacta cttctgcacc
   219301 ggtagaaact attgaaacca gaaaactgaa aaaaaccttt aaagtcctta tttggatgtc
   219361 tgctattgtc ctactattca ttattattaa atatgtagct tttggtaacc gtcccaaatt
   219421 tacttctgta gcagatagca cgcaagtgga aatcccagaa ggacctctta ataaaaacct
   219481 acctattgca atgatgttag ttcctgccga tactttagtg atgggaaata ttgctcccga
   219541 ggcagatgat gatgaatttc ctcttttaac tattagtatt ccagcttttt atatcggcca
   219601 aaaagaaatc agtcaggcag aatggatgat ggtttttccc aataatccgt ctcatagtaa
   219661 agatcctgca ttacctgtgg aaaatgttag tttttacgat attatagaat tttgcaatca
   219721 gaagagtgtt ttagacggtt ttgaaccttg ctacgattat tacgataatg aagtggtttg
   219781 caatttttca tcaaatggtt atcgcttgcc cactgaggcg gaatgggaat ttgccgccaa
   219841 aagtgggaaa agatatgatt tttttgttta cagcggttcc gctgatcctg atgaagttgg
   219901 ttggtataat ggcaatagcg aagctcaaag tcatcctgga ggacaaaaaa aacctaacca
   219961 actgggaatt tatgatctaa gcggaaacct gtttgaatgg gtctggaatt ggtatgctcc
   220021 ttattcagca cgcaactgga atttgataac tggtccagat aaaggaaccg ataaagttat
   220081 tagaggtggt tcttggtatc atagtgtttc cgaaatgaga gttactaacc gaaattatgc
   220141 taaaccttac acgaaaaatg cctaccttgg ttttcgagta gtaagaacaa aatcttaaca
   220201 agttcaaagt tcagagttca aagttcagag ttcaaagttc agagttcaaa gttcaaaagt
   220261 tcaaggttta aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
   220321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaagttca
   220381 aagttatatt tggcaaaacc gtgcaattct tacaattaaa ctctcgctgc gttagctctc
   220441 attacttcat ttctacttga cagcaaacgg cttaccgatg tttaggttat aaaaataaaa
   220501 taagaggaaa aaccaagtgg aatacactga ttataaaaaa acagccaatg aaataattga
   220561 acaaatatct gaaattcgga ggttactttg atccggagcc gaagaaatta ttaatctccg
   220621 aattagagca aaagatgaat gctccggatt tttggttaga tcaaacaaat gctaaaaaag
   220681 taagtaaaca gctttctcaa ctacgagacg aactagatca cataagaaaa atggaaaata
   220741 taaaaggtga actggaaact tatctttctt tgctggaaga ggattttaat attgatttac
   220801 taaatgaagc agtttcggaa cttccacgca tacagaattt catagagaaa gcggaaattg
   220861 aatgctacct gaatgataaa tatgatcata atgatgctct gctaactatt cattccggtg
   220921 cgggaggaac agaatctcaa gattgggtgg aaatgcttct aagaatgtat tctcattggg
   220981 cagaaaagaa taattatagt tttaatatta ttgattacct accaggagaa gaagctggaa
   221041 taaaaagtgt aagtgttgaa attaaaggtg attttgctta tggaatgttg aaaagtgaaa
   221101 taggcattca tcgcttggta agaataagtc ctttcaacgc tcaaggcaaa agacagacct
   221161 cctttgcctc tgtttttgta tatcctgaat ttgatgaaga cctggatgtg gaaatagatc
   221221 ctaaagactt gaaaattgat actttccgct caagtggagc aggaggtcaa tatgttaaca
   221281 caactgattc tgcagtacgc attactcatt tacctaccaa tatcgtagta acttgccaga
   221341 atgaacgcag tcagattcaa aacagagaaa aagcaatgtc cattctgaaa agccgcttat
   221401 atcaatacta tgaagaacaa cgggaaaaag aaaagcagaa tgtagagagt agtaaaacag
   221461 aaattggatg ggggaatcaa attcgcagtt atgtcttcca accatatcag atgattaaag
   221521 atcatagaac taattatgaa acgggtaata ctgataaagt tatggatggt gatttgaatg
   221581 attttatcta cgcttggttg aaatataatg ccaaacagag gataaatggc taaacaggat
   221641 tataaacaat tgctcaacga actggatagg gacaaattac ctaagcatat cggttttatt
   221701 atggatggta atggccgttg ggcaaaaaaa agaaataaac ctcatcttta tggtcataga
   221761 gccggtgtaa agtcattgcg tcaagttgta gaattgggag tggagctaaa actacaatat
   221821 ttaaccttct atgccttttc tacagagaac tggagccgtc cagaaagtga agtaaaaggc
   221881 cttttatggt tactaaaaga ccagctgaaa aaagaaattc cggaactgat ggaacaaaat
   221941 atctatgcac agtttattgg aacttctatt ggactggaaa aaaattactg gaatgaagta
   222001 agttcgcttg tggctcaaac tcataagaat acaggaatga tagtgaattt tgccttcaat
   222061 tatggaggtc gtctggaaat aattgagggc ttcaaaaagt ttatgtcaat gaacccggac
   222121 aaatgggata aattgactcc cgaggacttt ggaaattatc tttggacagc aggacaaccg
   222181 gatcctgatt tgataattag aaccagcgga gaaaagagac tttccaattt tttgctatgg
   222241 caatcagctt atgccgaaat atacataaca gagaccttgt ggcctgattt tgataaaaca
   222301 gaaatgatta aagcactttt agattatgcc tcgcgtgacc gtagatttgg aggtagaaaa
   222361 gaatgaatga gttattcaaa agagtgcttg ttgctgcttt attaattcct gtagtcctta
   222421 ttattcttta ttttcagggc ttatctctag ttatcacctt agcaattata gtttttttgg
   222481 gagggttgga atatataagg atgatgcata aagcaaaaat acaaataagt tatgtctgga
   222541 ttgtgttaaa ttgcttgttc tattttgcta ttgtatatat aaagggcaga gatttctctt
   222601 tgttatggtt agctttactt tttgctctaa tagaatctat tactacttgg aacgcagagc
   222661 gttctattcc acgacttttt gctacttttt tctgttttgt atatactgct ctatttcctg
   222721 ctttaatagc tcgtatcagt ttatcttatt cagaatataa ttttttactt gccttaattc
   222781 tgatgatatg gtttgtggat tcaatagctt attttgttgg tatgaaaata ggaaaacaca
   222841 gaaatataac agcaatcagt ccgcaaaagt ccttagaagg ttttcttgca ggaattcttg
   222901 cgccctggtt gattttgatt atattatata tttgcaaggt tagaattatg tctttttcct
   222961 atctggcttt aattgctatt gcagcaggca ttattggtca gattggagat ctgattgaat
   223021 ctatgttaaa gcgttattgt aaagtgaaaa atagttcgaa tctgcttccc ggtcacgggg
   223081 gaattttaga tcgttgtgat agtattctat tcgcaggttc gttcttatat tgtgctttgg
   223141 aaattttaac aaaagtgagg taataataat gaaaaaagca cttatgctaa tcgtcttaat
   223201 ggcagcaact gcagctatat ttgctcaagc tgttgatctc ttcttttctg aatatgtgga
   223261 agggacaagt aacaacaaag cattagagat cttcaatgga actggtgatc ctgtagattt
   223321 atctaactat acagttaaac ttgcttccaa tggaggaacc tggagtacta ccaatatttt
   223381 gcatatgact ggtgttttag atgatggtga agtttatgtg attgctaatt caggagccga
   223441 agcttctatt cttgctatag cgaatgtgac ttctacagtt acttattata atggcaatga
   223501 tgtagtaggt ctttttaatg gtgatactct aattgatatc attggtgtct atcaacagga
   223561 tcccggacct gctaattggc ctgtagctgg aactccttcc ggtactgctg aacatacttt
   223621 aatccgtaaa ccagatgtta tacaaggcaa tactaatttt attgctggtg caggaacaaa
   223681 tcaagatgat tctgaatgga tagttcatcc gcagaactat tttgctgact tgggccaaca
   223741 tacttttaac cctagtgcac ccgaacaagc tgctactcca gtttttaatc ctcctgcagg
   223801 ggtctatgct aatcctataa atgtaactat tacttctgaa actacaggag ctgaaataca
   223861 ttatacaact gatggtagtg aaccaagtga atcttcaacc ctatatactg ttccaatagc
   223921 tatttcatct acaacaactt tgaaagctaa ggcatttgca gctggtttta atccttctta
   223981 tacagctacc gctaattacc tatttccagt tcaaatatcg aatatagcag cccttagagc
   224041 ttcgactgct gacggaacaa ctatctatca actggcaaat gaagttattc ttacaatgca
   224101 acagacttgg cgtcatcaga agtttatcca agatgcaact ggtgccatac ttattgatga
   224161 ctatccagga gtaatcatca caacttataa tattggagat gggattacag gcatcactgg
   224221 aactcttaat cgttatacaa ctggcatgct tcagttttgg cctacagtag atcctggacc
   224281 tgccacttct tcagggaatg tagttattcc gcagttggta actataacgg agcttaatca
   224341 tgatattgag ctttatcaat cacggttagt tcgtctgaat aatgttcatt atgcatctcc
   224401 cagcggagat tatgctgctt taaccagtta tgatctgtta gatgatacag gaacagttgt
   224461 gatgcgtact caattttccg atgcagatta cattggcact cctatgcacc agggtaattt
   224521 tgatgtgtat gtgatagtta ctcaatataa tactgctgtt caagtaactc ccaggatgct
   224581 tagtgatttt aatccggttg ccaatgatga tgaagttctg atcccgagga acattgagct
   224641 tttaggtaat tttcccaatc cttttaatcc ggaaactact attcgtttca ggatggacaa
   224701 atctgctcct gccgaattga ctatttataa cgaaaaggga caggttgtta aaactataaa
   224761 cactattact actcagggta ttaatagcgt tgtttgggac ggtaaagatc ttagtggaaa
   224821 gcaagttagc agcggtgtct atttcttccg tctaaaatct ggcagctaca gcagcacaaa
   224881 aaaaatggtg atgatgaaat aaccggagga cactgttcct ccgcaatata gtgtagtcgg
   224941 aggacgctgt tcctttgcaa aaatgaggta aagacagcga gctccgacta caaagaaaaa
   225001 cagcatactg acaacctatt ttttacaaca tacaataaag cacagatttc agtatactgc
   225061 ttcattatta agtgatagat gcaaacaccc caaaaaaagc tactgttaat tacggattta
   225121 tatcccaata aatttaatcc tgtaaatggt gtatttgttc agaagcaagc aatcgagttg
   225181 tcgaagttct atcaggttca ggtggtagct gcttgtgaca aaaatcctta tcttatttat
   225241 caacatcatc aggatggttt tactgccaag ataatctgct atccctattg gcaaaagttt
   225301 tttttatctt ctctgataac ttatagaata ttagctttac ctgcaataaa aagatgctat
   225361 aaaagatttc aaccggatct gatacatatt cacgattacc gtcatatacc agaattattt
   225421 tggttgaagc cctggctaaa taagcttcct attccaagat ttttaacctt acataatatc
   225481 cgaactcatc cagcttttct aataaataac cccagaatta agttctataa atgggcttta
   225541 caatggacat taacaagatg gaatcatata tttactgtca acagcagatt ggctgaatgg
   225601 gtaaaaacct ataatcagga agtaacagtt atagggaatg gtataactga accgatatct
   225661 gttgaacccg tattaataga caaatttaag aaaaaactat atgatgatta ttttaaaatt
   225721 atcagtattg ggaatctggt taccgagaag ggcttttcct atttgattga agcagtttct
   225781 attctgaaaa aggaagggtt ccttatccaa cttttaattg taggagaagg cgaaaaaaga
   225841 aatgatattg aacgcaagat tcattcttgc aatgttgcta aagaggtaac cttaccaggc
   225901 agaattgata atgctttctt aagaaatatt ctgcctttgt tcgatttatt tgttttacca
   225961 agctatagcg agacatttgg cattgttttt ttagaagcga tgtatgctgg attacctgtt
   226021 attggaatta gaaatgaagg tatttacggc ttggcagaag atggggtaga ggcacttttt
   226081 gcagaaccta aaaacagtaa agaccttgct gacaaaatca aatttctgat aaacaatcct
   226141 gacctaaaag aagaaatagc aaaagccggg cagagattag tgaaagaaaa atatatgctt
   226201 aaggaactta tccaaagagt aatagccatt tatgagcaaa gataaattac tgatgattgt
   226261 taacgaattt cctcctacag gagcaagttc tgttcagaga cctttaaagt ttgccaaata
   226321 tgctgttaaa gctggttggg aagttcatat tattgcacct aaaaaaccgg taaggaaggt
   226381 cttggattat tccctgttac aagaaattcc tcctgaaaca aaagtataca gagttgctgg
   226441 tttgggcatc aaaaatcccg aagaaaacaa aatggtagat gccaactttg aagatacagc
   226501 cccccaatca aaaataatca aggcattttg gagattttta aagctaatca atgattttct
   226561 ttttccctat gataaacaga ttggttggat gccttttgct tattggaaaa ccgtgaaatt
   226621 aatcaataaa tacaaaatta gaaatatata tattacagct tatccctatt ctgcattttt
   226681 ggtcggtatc gcactaaaaa agaagtataa agatagtata ttttggattg ccgactatag
   226741 ggattcttgg cagtttggac ccttacttga taagatggtt ttacctttta gatttaaaaa
   226801 aatatgcaga actgatgata gggttttggt aacttgtgat gctgcagtat ttgttactcc
   226861 tgaatcccga aatatatatt tgcataagca tccctggctg gataaaaaaa gttactacat
   226921 ccctaatggc tatgatgagg acgattttga aggcgttatc cctaaaaagt ttgattgttt
   226981 aacttttgtg tatatgggca agctaaccag ggtatatgga aatcccatat ctttattaga
   227041 agcactaaaa gaatgtaacc tgcctaaata tcaggttata catattggaa gcataagtgc
   227101 cgcttttctg caagatatcc aaagaataga taagggagtt tataaattct gtggttataa
   227161 aaatcaccgg gaagctatat cttacattct gggagctgat ataaatctga tttttctaaa
   227221 cgatgatcca atttcaactt ttagttttac agggaagcta tttgaactac tgagagctgg
   227281 aaagccaatt ttagcgatgg gacccaaaaa atgtattata gaggacttac taaaagaaac
   227341 aggcgcaggc aaatatgcct ggataaagga taaagaccaa attaaaatga agattaatga
   227401 attgctaaca gatacatctt cgttttctat tcaaaccgat attattaaac aatattctcg
   227461 cgcagaaacc tgcaagacac ttttatccat atatgaaaga ggtcaaatca gtaattcttg
   227521 aacgcgctta aaaaccatat caggagtaag cttaaccaag caatctaatg cacacttagg
   227581 tttctttttc ttttctaggc aaggagcaca ggagacagga taacgaataa tatggctatt
   227641 agcagaaaaa ggagctgttt gagcttcatt ggcgggtcca aaaatcgtta aactatttat
   227701 cccaaagcag gaggcaatat gtcctaagcc agaatctgta ttaataaaga aatgtaagtg
   227761 cgaaataaca gctgccacat ttgccaaagc tgcattgctc aaaatcaagg tgttcgttct
   227821 tgcctgaatt gcttttccta cttcaatttc atccggtcct gcaattatga gaaccggata
   227881 ggaataatgc tcttgcaatt tgtttatcag agttataaaa tattctttac tccatcttct
   227941 atgtttgttt ttagctaaac aaccaggatg aataccaata actttttctc ctgaaagttt
   228001 gttctcgagt aagaatttct ccgcccactt ctgattttct tcggtcaaat gaaaataagg
   228061 atataaatgt aagtcatttt gcttggaaag tgcatcttcc caagaaggta aatttaaaaa
   228121 actctcaaac agtgcataat tactacgggt tctggaaaat gcaggattga actttatata
   228181 catagataaa ccaggatagc ggatagttct atattcacct atcttttttt cggctttcaa
   228241 aagtaacata aatagccatg ttttccaagg ttttactcca gaagaaaaaa gaactgctcc
   228301 atatttttgc ttgcgcagag ccaaaattgt gggaataagt aagttagctt tccaaccggt
   228361 aaagtgtatt ttacttataa agggagcatt tgtaacagga attgccgctg attcaggaaa
   228421 gaaaagaact tccagttcca gttttggata catttgtttt acagcctgca ggacaggagt
   228481 taataatatc aaatcaccaa taccccaagt gtgaattatt aatacttttt ttactggttc
   228541 atctttcata ggtctatcct taagctttct tgtgggatat attattcatc atctctttaa
   228601 tttcgattac tgtctctcga tagaggtcag ttacatcttt ccaggtaggc ataagataat
   228661 gcaacatctt gcctttacta tcacgctgtt caaaatagac aatcaaaaga aaccaaagaa
   228721 aataagatat actggtagca agagcagcac cattaattcc cattgtaggt attaacagca
   228781 tattcaaagt aatattcaat accaaagcta ttgcaggaag gataatactg attatcggaa
   228841 aacctttact gttcagataa ttattaaata gagaaccaaa actaagtcca taagccgcag
   228901 gcatcaacca aaaataaaca taataaacag gaacgaaatc agctccaaac attatctgca
   228961 ttactggttt tcctattaat ccaaaaccaa gattcgccaa agtaataaaa aagaaaaaaa
   229021 tcaatagggt tttctttagc agaagccatt tacttatgtc atcttcaata tcggaaagct
   229081 tgacaaataa taagccccct acgaggttag aggcaatttg aataaaatct acaatatgag
   229141 cagccagaga atagatacct acttctgaga aatccagaaa gtgcttaatt aacacaatat
   229201 cggctcgaat taacagcatt atgaataatg aggatagaaa aactcgaaat ccataactat
   229261 agaacagctt gattgttttt atattcaatt ccttatacca atttccccac cggttacgga
   229321 aagcaagata taaaacactg caagccaagc caatcaagtt aaataacaac aggatatcaa
   229381 ttctatcaac atctataagt gcaaaatagc cgattaataa aagaataaag aatacaaccg
   229441 agattaaaaa atgagagatg gaattttcca gtaccctatt catccccata tatattgcct
   229501 gtagttgcat aaatgctttg ttcaaggaaa caaacataac cagaaaaatt atttttactg
   229561 ggcttagctg atagccaatc agtttattcc aaaaatccag aaacgcaact ccgatagagg
   229621 tcattactac tatttcgaag aacatagtga tgattgtcca tccaaacaat gtctgaactt
   229681 catctgggcg tttacgcact atataaggat aacttctata aacccctaaa tcaagaactg
   229741 tccacataaa accgcataaa gtgatcagat aactgtattc accctttaag gttactccta
   229801 aatatctggc actaatccag ctgatcagaa atgacaatat caggattact attctgaaac
   229861 tgccgcttgt tattatattt tttttcagat ttatctggga catttttctc ttgggtaatt
   229921 agcactaaat tttctattat aattcataat gccataaaca taatccctgc ctttttgtca
   229981 attgtaaaac taaattcgat actgttagtg gtagtgaatt ctgcttgaca gctcaaccct
   230041 gttaataatt gtgatttata aatattgaca gattgttaaa ctgttggtgt ataggatgta
   230101 aaggaaaata aatgaccata gcgaagatag tattctgggt ttcatggctg cttttagctt
   230161 accatctttt cgggtatgga ttaattttat tcatattcaa cgctctgttt acaaaaaaga
   230221 aatctcttta tcccgaacct gatgaatatc cttctattac ggtactatgt cctgcttata
   230281 acgaagaaaa gttaattgaa gcaaagattc aatctttttt aaacttagac tatcccaaag
   230341 ataaaataaa aatgatagtg atttctgatg attcaaccga taaaaccaat gaaatagtag
   230401 aaaaatacac caattataat atatctctag taattcaaaa accgagggca ggtaaacaaa
   230461 atgcgcataa tttagttttg ccaatgcttg atactgagta tatattatct acagatgctg
   230521 attgtttatt tgaacctgat agcgttaaaa tactggtagc taatatgcta tctgatagaa
   230581 gaatcggttt ggtatcgggt gctgtaaaaa aagtcgataa aggaaacagg gaatcgggag
   230641 aagggattta ttggaaatac gagtcatttc tgaaattaat ggaatcaaaa tttaaaacga
   230701 ttattggtgc tactggagtt atgtatttaa ttaaaagaga actatttact cctataagtt
   230761 ctaatagccc cgatgatttc gaaagagttt tactgattat aaagaatgga tattatgcaa
   230821 cttatgaacc tcgagcttca atttcactgg aggttacaga aaaagcaacg gaagaggttc
   230881 agagaaaaat taggattatt acccaagaat ggtttgtttt aaagcggaat aaagaacttt
   230941 tgaatccttt tcgctatcca gctgtcagtt ttatcttctt ttcgcacaag ctgttgcgtt
   231001 ggttgttttt tgtttttgtg ttgacaggat tcatttccaa cgcttttttg atctcagttg
   231061 gtttttacag ggttgttttc atcctgcaga tagtagttta tctacttggg acattggggc
   231121 tcataagtca agcaaaagga aaacacatac ctctaaccgg tttggcagga tattttgtag
   231181 ctatggtata ttcttctgcg gttgcctttt tccatttcct gcaagataaa aagtatgatc
   231241 tttggaaccc tttacgttaa tggaggataa atgaatatat taattacggg cagtaacggt
   231301 tttgttggat ccaatttaat gtggaaatta gaagaagagg gacatcaagt tatcggaata
   231361 gatatcagtg accaatgcga ttataaaaaa cattcggaaa cgatgatagg tgatatacgg
   231421 tctctgaaag agctgaataa ggtctctgat acttatctta aaaagtataa cagccctata
   231481 gatttgataa tacattgtgc tgctgcaaaa aatgatttcg gaatttccca cgcggaatac
   231541 tatagccata ataaatatgg caccaaaact ctaattagtt ttgccaatca gcagaatatc
   231601 aataaaatga tctatttcag ttcagtaggt gtttttggac accctcaggg agaagctaat
   231661 gaggattttc cttataaacc tgacagtgct tatggcgctt ccaagctggc tggtgaactc
   231721 ctttgtataa aatggcaaca ggaaaatcca aaacgagaac ttgtagtatt gcgccctgca
   231781 gctatttttg gacctcgcaa cattaccaat acttataaac tgattgatac tctacataga
   231841 agaccatatt taacaatagg tgagggaaat catataaaaa ctatcgtttc aatatatact
   231901 gttattgata tgacttgttt tgctatccag caattaaaac caggattcga gcattataac
   231961 tgcatagatg aaccgtattt aactcttaaa gagctgatgg agcttatatg tagtaatccc
   232021 ggttttaaaa tgccgcgaat taaaatacct ttaaaagcag ctatcggtat tggatatctg
   232081 tttgatattc ctgctaaact tttttccata gacatacctg taaacagtga tagaatgcgt
   232141 aaattgggaa ctgctaccta ttttacagct gaaaaagcaa aaaaagcagg ttttattcaa
   232201 agagttccac taaaagatag cattgtcgaa atgtgcaatt ggtatctatc tttaaatgaa
   232261 taatatgaag gagttttatt atggaaacaa ttaaaatagg aataattggc tgtggccgta
   232321 tttctaaaaa ccattttgat gccgtttctc aaatccagga agctgaattc gttgctgctt
   232381 gcgatgttat tgattcaaga ttgcagaccg tttccgataa ctacggaatt aaaaaacttt
   232441 ataccaatta taaggagatg atggaaaaag aaaatttaga tcttgtttct atctgcaccc
   232501 caagtggctt gcatcctcaa ataggtattg atgcagccaa tcatagaatt aatgtgttaa
   232561 cagagaaacc tatggctact aatatcgaag cagccgatgc tctaatcaga gcttgtgatg
   232621 ccaataatgt taaacttttt gttgttaaac aaaatcgcct taattctact atgcaactac
   232681 taaaaagagc aattgataaa ggacgctttg gcagaatcta tttagctgaa tcaaatgttt
   232741 tttggcaaag acctcaagcc tattatgatg cagaaaaatg gcgcgggaca tgggaatttg
   232801 atggaggtgc ttttatgaat caagcaagcc attatgtaga tgcactttat tggcttttag
   232861 gaaatgtaga taatgttatg gcttacacag caactatggc acgcagaatt gaagctgaag
   232921 atacgggttg tgcaatcctc cattttcgga atggtattat tgccacttta aatgttacta
   232981 tgctaacttt tcccaaaaat tttgaaggat caataactat aattggagaa aaaggaactg
   233041 tgaaaattgg aggagtagcc gttaacaaaa tagaaaaatg ggaattcgaa gattatgatg
   233101 atgatgatcg cattgcccaa gatgctaatt atcaaccacc taatgtttat ggcttcggac
   233161 ataaccccta ttaccgcaat gtgatagatg tacttttagg taaagatgta ccttcaactg
   233221 atggacgcga tggcagaaaa tctgttgaga tcattcaggc catttatcgt tcagcaaaaa
   233281 ccggtaagcg tatttctcta cctttataaa ctgtaattta tagctggagg atttcgttcc
   233341 tccgcttttt ttttctagcc accgaagtca ccgaagattt ttcacgcaga ttacgctgat
   233401 tacgcagata taactttaga aacaaggatt aagaggattt agatgtgtag acggaggacg
   233461 ccgttcctcc gcattttttc ctagccaccg atgtcaccga agatttttca cgcagattac
   233521 gctgattacg cagatataac tttagaaaca aggattttag gattaagagg atttctaatt
   233581 taatatccat taggaacgat acatactaac gacggatttt aacccggaga caagaagaac
   233641 gaataaaaaa agtcccatcg ggacgacaga tttgagcgat ggattttaac ctggctaaaa
   233701 tacaggaagg ttgatgtttt catcaaccca caacaaacac actataaagc tatcattcaa
   233761 tattgcggga attacacaat ttgactgtaa gaggagagac aggatgtcaa tctttccaaa
   233821 aataactcac gcagattcca cagatatcag caactttagg ttcgaaggta tcgctatctt
   233881 taatatactt ccttttaagt agattttttt acgcatatct ttctccttaa ttttagtccg
   233941 ctattttcag gacacctcac tcatttctgt atctcctttc tttgttcttg tgtcaactta
   234001 tttaggatga aacatataac ttattcgaaa ctcataagca tcccatagca ttcccttatg
   234061 attcccatat cggataaggg aatgatacgg gaatgtatca aggcctatat ggttagagta
   234121 tatgttagga tcattatctt gcatattgaa tcccggaaaa aaacaaaagc taagtgaaaa
   234181 tctactatct tccttttggt attcatataa agggatgcta atttatttta cgcataatga
   234241 ttgccatcgt cccgatttac aacattaaat ttgtttattg caatattggc attattatat
   234301 agatagacca aaaatctata aaggagataa taatggaaac gagagaagga atgccattgt
   234361 tgggggattt tttccctgag atgactgtcg ttactacaaa aggtattatg aaccttccta
   234421 aagatatggc tgggaagtgg ttcgtttttt ttagccatcc agcagatttt acacccgtat
   234481 gcacaaccga atttgttgct tttcaaaagc tatatcctaa attcaaagct ttaaatacgg
   234541 agcttattgg gatgagtgtt gatcaagtgt tttcccatat taaatgggaa gaatggatta
   234601 aagaacacct tggagtagag attcaatttc ccataattgc tgatactggt gccatagcaa
   234661 acaaattagg gcttattcat cctggaaagg gaactaatac tgtccgagca gtattcattg
   234721 ttgatgataa aggcaaaata agaattattt tatactatcc acaagaactt ggacgtaata
   234781 tggaggaaat tctccgtgcc gttgaaggaa tgcagttctc agataataat gcttgttcta
   234841 tgcctgccaa ttggcccaaa aatgaactga ttggagataa ggttattatt cctgccgctt
   234901 ctactgttga agcagctaat gagcgtctcg ctaaaataaa gaaaggggaa attgagggtt
   234961 atgattggtg gttttcatat cgcgatgcaa aagctaaaca ggtaaagaaa caggtaaatc
   235021 cttctaaagc aactaccaag agtagaaaga aataattgtt tatgtgggca gcgtaaaaaa
   235081 ctgccctttt tttatcccat aatatcaaag ggaatgtcct aaaaatactc tttgtgatta
   235141 atgcgtaaac aagttacata aataatctca caagcgccat taccctcgtc tgttctggtt
   235201 gtacttgtgt tccaagaact aaaacgcttt ttcttatccc gacctagaac attaactaag
   235261 tgcccttcca gttgtactat atctttcttt tttacctttc ccatagcctg ttttatattc
   235321 ttgtttgcag gaatcagatg attattgcta aaatgttcat ttaagtattg aatatcaatg
   235381 ggcgctccag ttttatattt atataaacaa aatcttacta tctgatcaaa tttcaaataa
   235441 ggtagatact ctgatgtttt tccccaaata agagcaaaat catagggcga taaatcactc
   235501 ataaatccgc gatgatatct ttttttgctt actaccattc ctgctatccg atactctgct
   235561 agtgggatta tagtatattc tctactgcct aacacaatat ttgggatata ggcatcttta
   235621 ttaacctttt gaacaggctc ttcagaaagg tctataaaag atgaactaag gcaattcctt
   235681 ccttcaggag atgttactgc aaaatataga taaataaagg ttatcgatac aataaccaaa
   235741 caactaatca caataaaacg atttttcaac tgtacctctt ttgtcatctt atcataatgt
   235801 aacgctttga agcgatatgg caaattaaac ttttctctga ttaaactgcc atgggaaaga
   235861 tactgataag ctggcttgta ggctattaaa aagaactgag gttattagaa aggcaaaatg
   235921 gatctatctt actaaagaag aaaaatctag tttcaacaat ataaatgaaa atggaatata
   235981 gattgcttat tattataaag aaataaatgt catttgtcct ttaaagaact gaaaagagga
   236041 gacaataatg aaaaagtctt ttctaataat ggtatttatg attgttatct caattttaaa
   236101 tgccttcgaa agagtgccaa tatatagctc tcaatattta gaatatgatg gaaacggtat
   236161 tacactgccc aatggagatt ttttgttttt tactaatgcc tctactaata acggtttaaa
   236221 tgtaatgatg aatagagtat gtgttaatgg aacaaatgtg aattcagaac catataatat
   236281 tactaatgct cccggtgatg aatccattat acaacaagta ttaagctcag acaattgcct
   236341 catttttatt tacaattata ttaatccact agtgtcttat gatgatagtg aaatatatat
   236401 tcaaaaagtc actttaaacg gagagctctt atggggtgaa aggggaattc gattaggaat
   236461 acctagttat aatagattca aattggtacc caataataat ggaggtgctt atgttaccta
   236521 tgtcctcgtc cgaaactatc ctaaactata cggatggaat tttgatgctc aaggtaataa
   236581 ccaatggcat tcggaatttg tttttgaact gcaacctccc aatttgtttt atctttccaa
   236641 tgtcgttagt gatggagacg gaaatattat cgtaaattta tgggtacagc ctgattcaaa
   236701 cagttatcta tacagctact tggtgaagat ctctcctcag ggaaatacta ttggtaataa
   236761 tccgatggtg acccaaagtc aattttcagg ttgttatgat attttcagtg cgcagaatgg
   236821 taattatttc ctatggaaat ccaatctttc cagtattact tttcaaaaaa tggatgcttc
   236881 aggtaatctt cttttccccg aaccaatatc ttatcaatat gatgaaaatg tttcggaaat
   236941 tagtactttt ggttcttcag cagatggggg tttgtattat tatctgaata gagaatatcc
   237001 tttgtaccct atgctctgta aattgaatgc tgatttacag ccaagttggt ctgctcctat
   237061 agaattacct ttttattgga attattataa tggaacttac aggattatgg atgataattc
   237121 tatatatatt gcttatgtgg attacataaa ttcgtactat ccaatttatg attctggaat
   237181 tatgttaact cacattgatc ccataaacgg tacttattca tttcctgcaa cttatatttc
   237241 caatatggtt tttcccaaat acggagttca tatttttaat tcttctgcta atgcactggt
   237301 ttcgtggagt gatttaactg atggaaattt tgaagttcgc tgccaaatag ttgatccttc
   237361 tggttcaatc ttactgcaga ctgaaggaga aactcttcaa tacagacact tcgggtctgt
   237421 agaaaaatat caagcagtgg agattttaga aggctcctgt tatgttttcg aaattaataa
   237481 tatcgatctt agtagtaaac tctattatca aatctgtaat gacaaccttt cgccattgct
   237541 ggaagaattt ggtagagaac taaatcctga ttctgatgag atagaactgc taaaagccat
   237601 tgttagaact ctggataatc agataggtat cctttatctt tctggtaacg aatatttcaa
   237661 tttatattac caagtgattg ataatatggg tattgctcta ttacccggga aaggagttct
   237721 aataaaagaa aatgtggcaa atactaattg taacttttgt atgacttgcg ctgatggtga
   237781 tgtaataatt gcctgggaag aagtttattc tctgctctac acacaaatta ttagaggcca
   237841 atgtttgcat aataatgttc cccaatgggt ttccggaggg aaaattctta agcaaagcac
   237901 ccctaaggga gttgccttaa ttaaaatact gggaaattac cttgtctttg cggattatgc
   237961 gctagattat tcattaggtg taataaaatt ggatgcaaat ggaaaccctg agccatcttg
   238021 ggcactaact ggaagttata ttacaagcgg aactaattta gatctaatcc agaactgtgg
   238081 attaataaat gatgaacttg tgctgttcta ttataaccaa gtctctccag acataaataa
   238141 tgtgcataat ctatgtgttc aaaaattcaa taatttgggt tatggcttat ggggtgacta
   238201 tggaattata ttagatacaa gtgggagttg gggtatcgct tcaattgaag gtgctagcta
   238261 tggacaaaaa gtctttttct ctatgctaaa atataatgtg gaacataaca gatacgaaat
   238321 acattacaat gttattaatg atgatggaac tatgttatgg gaaaatacag aaagcatccc
   238381 tatacagtac atttatccat cttcgacatt acatcattta tcctataaca acgatgttaa
   238441 ctctcttttt tggctctccc gaactgataa ttatctaaaa gaaatcaagc atcattacat
   238501 tttacccgac ggattactat actataatga accagtacta attgaacaaa tagctaaagc
   238561 gattacaaac cttatgtcac tcaataaaga aacatatggt ttggctacct atataatcaa
   238621 tgattattat tttgaggata aaaaatttcg tggagaaaat gaaggattac caacttttgg
   238681 taataacccc aaagggatta gctacagtct aaattccctc tacgcctgca aaatttcttc
   238741 caatcctacg cttgctgagg atcccattca aaacccttca aatctatttt gtcataacta
   238801 tcctaatccc tttaatcctg aaaccactat ctgctttgat ttacctgctc cagataggat
   238861 tcacctaaat atctataatc ttaaaggaca attaatccgc aatcttataa atgattataa
   238921 accttcaggg aaacaatctg ttatatggaa tggtactgat aacaacaatc aatccgtggc
   238981 aagcggagta tatgtgtata aaattacaac aggaaaatac attcaaaccg gtaaaatgat
   239041 gctgatgaaa taaacaaatc tgtctcgttt gcctatctga attacacaaa aataccactc
   239101 acttcgtgga atttatacat ttttctcgcc attcaaaaaa ataatttgta tatttaacga
   239161 tttgcgttat aatatgttgt agaggc
//
LOCUS       DAOSTH010000002        87887 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_280_length_87887_cov_12.558418, whole genome shotgun sequence.
ACCESSION   DAOSTH010000002 DAOSTH010000000
VERSION     DAOSTH010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 87887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 87887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87887
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_280_length_87887_cov_12.558418"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(200..1660)
                     /locus_tag="PLB65_00930"
     CDS             complement(200..1660)
                     /locus_tag="PLB65_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOQ77118.1"
                     /translation="MKHKIAILVVICLLWSIALAATKPDPTALVGQVGDREYNYKKFN
                     DGFKAYLQYYGGGKVLTKQDSIKLNNQYWEELVGIYVYDQAIKAGKIKVTDAELEAEI
                     KKNIPSEVKQIKDFQTNGKFDNAKYLQALKDHPEFKKELMNYARDLYSYNKLIKTIKN
                     EVKANPDSVKNAWMHQNNYASAKIICFDYNKLTNINVTDEEAREYYEAHKEEFKRENG
                     RRFLFVRFTGALSKSEDMEKIAQDNKTLSTTLFNRAKEVGLKRAAKEMNLPLEESPYF
                     NPQDQIIPLIGRAPDLIAFAFNNPVGSIPDIFYAPTGDIFVLELNSEIPEYYIDFEIK
                     KQEMIIRANRTKRMFTMDNYVQEFMKRETPETYLEAAARDSIAIVEVDKLLADSDIRT
                     IGKIPALNEAILNTPLGSFTPLIENEKRWYLAYVTNRQLPDLTIWEKDKQNIIAAAEK
                     KMQEDHLNQWYIEQRQKVKIIDNRKDFYELYIPLKL"
     gene            complement(1927..4728)
                     /locus_tag="PLB65_00935"
     CDS             complement(1927..4728)
                     /locus_tag="PLB65_00935"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77119.1"
                     /translation="MKKYWLIILALTLVTAIFANGSRYEVVAYSEDFESGATGWTHYD
                     GAVSPNNWHIYNFGGTQSDVWWMGDPALAQGTYIGGYYNHQYLVLDTPARTLTAANAT
                     LTFKMRHNVETPSSNPPYDGWDACNVRISTDGGTTWTPITGTPAYDMTSAYSFGFEHG
                     EGTGIPAWGGVREWTNCTFNLSSYVGQSVKIRFAFASDPAYCTSEAPAMYGMMVDDIS
                     FGGYTNNGVNDGQMTWSSMVPQGGDIWHLGTDATAPSPSHCMICQNDAGSYNINMMNY
                     LVSPPIQLPESGDIRADFMIMGNFTDPNTFPEVDYWGWEMSVNNGITWHYMSNPYDDP
                     NGNNYVYSDAPPAWASMTESYTLDGLISNYHGETAIFRWFFKSDADTPNGTGIRIDDF
                     KIYNDIFIAAPENLEATVSGNSVTLNWNAPGSGGGGGEEGWIYYCGEPAGNSVGTNSA
                     ADFDVAAKWDPLGDVNSIYPYVGMNITKIKFVPAEPTSMCSYSVRIWTGSAGTMVYDQ
                     PVSTITEDAWNEVVLNTPYTIPSGTAIWAGFRCNTTGGFPAGCDSGPQVEGYGNMIRL
                     SGVWQTLTQQSATLTYNWNIQVYVADANGKEYVLGELPMNDTFAEGTFGVAYNEPVRG
                     RQITAYKIYRDETYLAQVEGSVLTYTDNEVPGGIHTYYVTAMYGTNESSASNAVTTFV
                     VPADHYELNHDDGIAEQGFSVGSTKQMAVKYSHSGQFSIKYIKAYVHTVGTAPMIVRV
                     YDNSGVDGTPGVQLTQYQYPLASIVEGWNYITLPTEVQLTDATFYIGIMETVNSSLIG
                     LDTNSSGYSYKKIGTNWEPVTEGEIMLRPIVMGGMAGEDPTLPALVLDAKNYPNPFNP
                     ETTIHFSLPESGPTSVKVYNLKGQLIRTLVNGNMTAGNQKVVWNGLDDNNQTVASGLY
                     FYRVTNVGKNITRKMLLSK"
     gene            complement(5019..7187)
                     /locus_tag="PLB65_00940"
     CDS             complement(5019..7187)
                     /locus_tag="PLB65_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOQ77120.1"
                     /translation="MIGGGGGGGHSGRGAHFEDELKNKIYDRRLFSNVIHYIKPYLKW
                     VILSFLFLMLISGAEVIIPLIQRSAIDDYIVSDKTLAIFTQEDDYHEFINRYQKLGFK
                     EYKYGDNHFLVISSKDRNKMDRADLEKLKAKGILKEDTVFLVSAKDKNISILKQYLPE
                     NINPEQPNLTGWFKISSETIAIPKEQLKELPKAKRLEVRSDASKQLLILALIFLAIIT
                     LRFVASYFQTVFTAYFSQTAMADLRHDVFAHLQKMPTKFFDTNPVGRLVTRVTNDIAA
                     IDEMLASGVITIIQDVVLIITIVILMLALNWHLALVSFSILPLVIWVIVIFRRKTRVV
                     YREVRKHLAVLNATLAEHIEGQKIIQLFNQYFHKRQEFADINQKYYLASIKQIRLFAF
                     FRPIIHVSSQIAVALIIWYGGGKILQNVITIGLLMAFTQYIQKLFEPINDFSEKFNFL
                     QSALASAERIFNLLALSPDDYREEKHQNIKLEGEIEFENVWLAYNENEWVLKDISFKV
                     APGEKIALVGHTGSGKTSIVNLILGMYPYQKGRILIDGKDIHNYSLADLRRNVGIVQQ
                     DVFLFSGNIKDNIALNNDELSDEEIIQVAKYVNADKFISKLPNGYNEPVMERGATLST
                     GQRQLIAFARVLAYNPSIFILDEATSNIDTETELLIQDALNKIIKDRTSIIIAHRLST
                     IQNVDRILVLHKGEIVEEGSHFELLDKQGLYYDLYRLQYT"
     gene            complement(7187..8938)
                     /locus_tag="PLB65_00945"
     CDS             complement(7187..8938)
                     /locus_tag="PLB65_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ77121.1"
                     /translation="MRTFDAVLPYLKKSYTRILGGLVMLILVDLVQLAVPKVMQYAID
                     HIQERTIDYRGLAWIGLIIFGCAIAVMVLRYFWRILIIGNSHLIEKSLRQDYYDHLLT
                     LSQNFFNKSKTGDLMAYATNDLNAVRMLFGMGLISAMDIVLMTIASFSFMVSINAKLT
                     LLAIIPMPIISLTVLYFGKKVHTRFKAVQESFATLSGKVQETISGIRIVKAFCQEKNE
                     LKKIDKVSHDFVDQNLALAKIAGVFHPFMGFIISISMIITLYFGGKAAIRGEISVGGF
                     IAFFQYLGMLVWPMIAIGHIVDMYQRGTASLKRLNDIFEVNPEIDDAFADERITHLKG
                     KIQFQNLSFRYADHLPFIFNDITAGIEAGKTMAIVGPTGCGKTTLIELLVRIYNPPRN
                     SIYIDDYELFTIPLNVLRRDIVLVPQDIFLFSETIADNIRLGNPEASDEEVFEAARTA
                     QVYDEIMEFEHKFDTIVGERGVTLSGGQKQRVAIARALLTNPNILILDDALSAVDTKT
                     ERRILNRLIEIRKGKTTFIIAHRISALQHSDLIIVINNGIIAEQGTHQELLAKGGLYK
                     DLYEKQRIRAKIEGEEE"
     gene            9736..10209
                     /locus_tag="PLB65_00950"
     CDS             9736..10209
                     /locus_tag="PLB65_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOQ77122.1"
                     /translation="MNKLFMPELVKIIEKFENKNACLHYMADLLSESGCLSFPDRFLA
                     AVKGREEIMSTGIGRGIAIPHARDLTVSSLKIAVCMIKQGLDFTSVDNLPVNLVFMIA
                     VPQSSNQDYMKILRSLSGFLRRDENRESLLQATDERDLYEKVQVLEEELRPQLGV"
     gene            10157..11128
                     /locus_tag="PLB65_00955"
     CDS             10157..11128
                     /locus_tag="PLB65_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOQ77123.1"
                     /translation="MKKFRFLKKSFALSLAFSFLIILPLFSQHQDAGTTGFSTLKIYY
                     SARANAMGQAMLGRAMNFEGMQFNPATLIKAKNKAVYTTFADHFVGSGGGSINYMVPK
                     NIYTSYGFYLNYWNSGSIDRTDIGANGEFIELNDTFGAQDITAGFSVAKFVSPALDAG
                     GSVKLVLDQIDGKSATAALIDLGILHHTPNEKIKVGLTIRNLGFQLSHYTNKDYSEGI
                     PITFGAGIGIDAAKNTLLNIDLNKANGENFIGKFGIEQSVHPSLVLRAGFKTNGGDYS
                     IGGDLGWSSGISLGLGWTWKKYAIDYAIASYGDLGFTNQVSIRYNFD"
     gene            11165..13258
                     /gene="glyS"
                     /locus_tag="PLB65_00960"
     CDS             11165..13258
                     /gene="glyS"
                     /locus_tag="PLB65_00960"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOQ77124.1"
                     /translation="MKQNQFLLELGVEELPEKQITIACNSVKLSFANFLKENKLNCSG
                     YKVSGTPRRIFLQAIDLDSHQKDEEIVKTGPAFRLAYDEAGNLTNAGKGFLKRNQVEE
                     SAIFIQETDKGKFLAVKYIKTGISTGELLQKWIPDMILQIPFDKKMLWNKPGFAFSRP
                     LRWLLILLNNNPLTIDFFGIPCGNTTYGNRYLGLDRCITINKAEEYIPKLLEHKVVAE
                     IDERRKEIVDQMANIFPKGDYRVIKDLPLVETVVNLVEFPTAVCGEFEHKYLSLPDKI
                     IISTISQAQKYFAVHDTIGKLSNKFVFISNGDPAAWDLIKQGNEKVVNARLADALWYW
                     NEDTKHPLDYWTKHLDMVVFQAKLGTMADKTKRIICLTELIAKELNLPSEQREKVLRC
                     AELCKADLVTNMLGEKEFTKLQGYIGKQYAIVSGEDEEVAEGIYEHYMPRGSADKLPS
                     TLCGALVAIADKMDTVAGIIGIGMLPTGSGDPFALRRAANGIVQIISHRKWDFNIFAL
                     ADEALELISKQTELDKNAYSNLHSFIEQRITGLLKTKGIAYDVIDSVMHIDKSHIYDL
                     ENRAKALNDLKSKPNFIHLVIGFKRVANIIAETTEFVPFDKNKLVEPEEINLYKSLQK
                     LHNDIDSALQKKDYPLALQYLISFGKVIDAFFDAVLVNCDDEVLHNNRHSLLREVKNE
                     FLRVADLSLIVLETE"
     gene            13271..14275
                     /gene="pta"
                     /locus_tag="PLB65_00965"
     CDS             13271..14275
                     /gene="pta"
                     /locus_tag="PLB65_00965"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOQ77125.1"
                     /translation="MFIIDEVKKKAKKTKGSVVLPESSDPRVLIAAAQIVKEDLAKII
                     LLGKEEKITEDAKALNLDLNGVTIINPETSEYLSAFADAFYLRRKHKGVNPVQALETV
                     KDPLYFGASLVKQGKVSGMVAGAVNTTADVLRAALQVIGVMPGLKTVSSTFIMPVPDF
                     RGEPKVFLFADCAVVPNPTAEQLADIAICTALTRKAIIGDEPKVALLSFSTLGSANDE
                     LVDKVISAKAILDERKVDFEYDGELQLDAALIPEVAIRKAPQSPIAGKANTLIFPDLQ
                     AGNIGYKLVQYIGKVKAIGPIIQGLAAPASDLSRGCSYEDIVNTVAYVLLLAAQQKYK
                     "
     gene            14300..15508
                     /locus_tag="PLB65_00970"
     CDS             14300..15508
                     /locus_tag="PLB65_00970"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOQ77126.1"
                     /translation="MATKLSNRCRLIKPSPTLSLSAKAKEMKDAGIDVISFSVGEPDF
                     PTPDYIKNAAHKAIDANYTRYTNNAGILELRQAICDKLLRDNDLKYSPKEILVSPGAK
                     ASIVNALTAVCDVKDQVLMGAPYWVSYPYQVALANAEPIYIPTKEEDGYKIRAAELEI
                     AIQENPCTKALILNSPGNPTGAVYTKEELAALAEICIKYDILVISDEIYERLVYDDVK
                     HISIASISEEMKQRTIVINGVSKAYAMTGWRLGYAAGPANIIATAGKVQEHSTSCVNS
                     ITQYACVTALNEEDDSIEKMRQEFSVRRDFLYNELKKIPHISCFKPQGAFYIMPGIKW
                     YLENNNQNIQTSDDFCAKLLDKYSVALVPGSSFGMEGTVRFSYANSMENIKEGISRFA
                     SFLQELNPGR"
     gene            15512..15727
                     /locus_tag="PLB65_00975"
     CDS             15512..15727
                     /locus_tag="PLB65_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77127.1"
                     /translation="MDDFNDKMNDRWKEKRKKAYNWPRLIIMILALIAILYIMNHLGN
                     TKNVVQTPSATVTDTLQADSVHKEITP"
     gene            15724..16617
                     /locus_tag="PLB65_00980"
     CDS             15724..16617
                     /locus_tag="PLB65_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOQ77128.1"
                     /translation="MSLVIVGSIGLDSISTPAGEVCEALGGSAIYGSLAASHFTNTHI
                     VGVVGKDIPVEYLNLLQKSGINLDGLEVKDGKTFRWKGKYSTWNRVETLSTDLNVFAD
                     FSPQLPESCKCCRSLLLANIHPALQLQVLNQVSGYSHLACDTMNYWIELCPEEVEKVL
                     KRVDIVFMNEDEIRDYTKIADIYSAAYEILSLGPKWVIVKRGECGSVAVSKDDMYFAP
                     AYPVKKVKDPTGAGDSFAGAFMGYLADHDFVNHALIKEAILYGTVLAAMNVSDFSVQG
                     LVGKSMSEIDNYKDRLIKWTS"
     gene            16605..17657
                     /gene="purM"
                     /locus_tag="PLB65_00985"
     CDS             16605..17657
                     /gene="purM"
                     /locus_tag="PLB65_00985"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOQ77129.1"
                     /translation="MDILKDSLDYRSSGVDIAAGEKAVTAIKHKVKATYNSNVLSELG
                     SFGGLYQIDKSSWQNPILVSSTDGVGTKLLVAILANEYKTIGQDLVNHCVNDILVQGA
                     IPQFFLDYIGLGKLIPARVEDIIEGMVVACKQNSCALIGGEMAEMPGIYKEKDFDLVG
                     TIVGIVEREQLLPRAKMKPGDKLIALPSSGLHTNGYSLVRKIVFEKLNLSLDTYIPDC
                     ETTLGELLLRVHRSYLPLLKDYLTDPSLLGLAHITGGGIAGNLKRILPDNVSAIINLK
                     ETDIPPFFKWLQEAGKVDFSIMKETFNLGIGMLCVVDHKMQDKYLSIPEARCVGELVP
                     SDYIQEKIEFDLCGAK"
     gene            17851..20883
                     /locus_tag="PLB65_00990"
     CDS             17851..20883
                     /locus_tag="PLB65_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOQ77130.1"
                     /translation="MRKYALILLIIMLSGVVFLDAATTGKLSVRVRDNSGRPLEFANV
                     VVSQGNQRITGGQTNEKGLAIIINIPPGIYTVKISLIGYDTVIYNDVRIKVDETTSLT
                     PTMTKSGIEMKAVTVTAKVDNVDKNQIGSARTIEMDIMTNAAVSDVSDIVSLQAGVVN
                     IGGELHIRGSRDNEINYTVDGMSVTDPVDGGATLQVDTDAIKDMKVMTGGFPAEYGNA
                     QAAVINIVTKDGDPFFSGKVEYNTDHLISEGRNSDVLKFAIGGPVLPFASQKMKEKFT
                     FYLNGAGEWLDGRLKDYYISDPNKDYSINGRSLIDADYEEYNPYSDRDNILGIDIGNR
                     NFNSYNINLKSKYAFKPGQVATFAIRGDRNLNYLYNNTWRYALQHYAEEETDQRQYIG
                     TYDRVINDRMNLKVKASYYQKDNYEGPRGIDRNNYMYMTIDPNNIPANYIDMVSLGNY
                     GYTTIDANNDNVHDVGFLPAEYWVYRLESVEEPRNIPGYNPPGTIYSRYVDDTTKIIS
                     TRADFEWQVNETHLAKTGLEVIKHNIMKNQLEDYLKIDEKRRTNYLNSIFNVRNFDLD
                     NYVDTNGDLLCDYVPEGLYALAAIQEVPTSLADLVPIYYPQDYYAAAKAAAGKRDGYK
                     AEPWQMAYYLQDKMEWEGMIVNAGLRFDFWYLGKDYSVARDDGSYDSRAFDESDRLQV
                     MISPRLGVSHPITDKDVLRFAYNYQNQLPQMQYIFTSKTPEDANDSDTIIQVGNPSLE
                     PQITITYEVGLSHQLSEDYVLDLTAYYKNLYNYVNTRKERSEIEQTVYWYRYYSNDYG
                     SARGIDMQIEKLLSNFNTWSLAYSLAWAQGNSSYTVIQDETTNLREFPLDWDIRHNIS
                     ANFTFHIARGEEYFIPFTDYILPLDDFSANFNWTFASGAPYTPQSAESNSSLDTNSKR
                     KDNTQQANLRLTKGFVLPNNMSIRIYAEIENLFKNMNVLNVYPKTGNYYWGGDNLEEA
                     NIPGFVYDEVQYVYSLAYKNPTYYNNFRGLTVGVSFNF"
     gene            20910..21674
                     /locus_tag="PLB65_00995"
     CDS             20910..21674
                     /locus_tag="PLB65_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOQ77131.1"
                     /translation="MRKFIGTILLIMMMTFVFASFAYAQENTAAADQGGGGLQNIAEL
                     VFGSGLVKWFKDGGWAMWPILFLAIYGLAYIIWKFIALLTGKVNLNAFLNKIVPLIKD
                     KKYEEAKEIASKTRGPVASIIYAGLEKIDKGIDAVDEAIENASMIEMSYLEKGFIELS
                     NSITLAPMLGFLGTVAGMITAFDSIAAARAVDATIVATGIKIALITTEAGLIVAIPVQ
                     FFNNVFMTMVDGIVIDMQRASEKVTEILAESIGTKK"
     gene            21698..22096
                     /locus_tag="PLB65_01000"
     CDS             21698..22096
                     /locus_tag="PLB65_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOQ77132.1"
                     /translation="MAAIPTASTGDIAFLLIIFFMSTTKFDVKEGIKLVLPQAAEVES
                     GKTQTLTLTEKEMTRIQILPDGQIALNREAPRYIESSELDVIIQQKVQINPDMIFKVI
                     TDRETKYSEMIRVVDRLKAAKIEKISLSTS"
     gene            22106..22540
                     /locus_tag="PLB65_01005"
     CDS             22106..22540
                     /locus_tag="PLB65_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOQ77133.1"
                     /translation="MAKFSTKKRRDVAIPQASTSDIAFLLLLFFMVTTVFVQEKKFWV
                     ERDRWPHAESIERIPRNHTSTIYVMRDETILLDDIPTRIESSEDVFISQTLIRKKAED
                     PELIVCFRTDRDTKYGVMSDIMRQLQDANTLVVAFETQPLLK"
     gene            22553..23188
                     /locus_tag="PLB65_01010"
     CDS             22553..23188
                     /locus_tag="PLB65_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOQ77134.1"
                     /translation="MTTHNQDWKDIANAQFSKALALSLTLLIFGIMVTPKVEVRKQVF
                     QTQQMELVDIPMEERERIEAPQTEVDVNVTFQISDVLGTEEVDMAAFQEALSQIGDIY
                     KTTTTSVQQQEDERVVNFVPYDDAPVIIGKIEPVYPEFAKRNKLQGTVVLEVEVLKDG
                     SIRDIRVRRGVGGGLDEAAIEAVRKVRFQPGKSSGQAVDCLVIIPVEFKIQ"
     gene            23203..26337
                     /locus_tag="PLB65_01015"
     CDS             23203..26337
                     /locus_tag="PLB65_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77135.1"
                     /translation="MKLYKIGICLCLLLIFSISSVYGAKAVPSTGTKKLDLTKYHNVG
                     NIWLRVSNYGFFGSGDDIVPRYPSLEYPGGSGMDYLYQGALWFGAKKQRRNNLNQKLY
                     WLTYPPSASNDTLITELDPRWNSNKVAALDTLVSVGFDGDDDLYEFLPAFNPLVVANP
                     EQSGNYALYNNQDGISYASTRQQKRGKDDDGDGRIDEDGAGFTFPFRMASELPIQFAN
                     DFGGRFMYEVPISAFSILDDPNNAEIWFPLGFMELSYRSNDPTYNYAFSAPFDDDGDG
                     RVDEDGAPVSEQDYISFYYDYCPFGTDGERDYGASKGGNTHIPIKVRVRQMSYQWSYD
                     YIKNLVYVEFDITNMNDLDELKDCAMGIYMDCDVGPQTYGVEKASDDKSGYVKGPGYE
                     FAYTYDADGDNGLCTGLVGARVCTPDPEKLSFDCWYWKVGQGPDDSRPRNLSPSNKTA
                     NEKYWLLTGRNPNTTYYTPLRPEADDQTEWEQPSPNDTRFLFSFYGAQPGTEEYNEQD
                     ENGNYHKRWNLAPNKTMKIVVAVFPGDNKEDLKRTARWAKEIYGQAQDLVTVVLPDTF
                     PHYNPPEPPEIPTMYAEMVNDGNQINIYWDNRSEFSYDVKTVSTAVMGWQDPNSANII
                     SGLDSDPRPHIANNWADIPEQFRPDESLPPDKCWNMNALVNPFTAYRLRHDFQGYTVW
                     GRSGSGSQEDWSMIDRWDKVDTAQDLADYSANYGYPDVFVDFGGYMGINTGLPNKNAW
                     NAPLSEYNKFYCFDENYSLVPNGDTFYGWPIYEPNPIINGTPMDELTDWSTIQEAADE
                     IVGPDMETTKLMQARLFKHNEIPQQIFDALYDPKLIPLRGFAFPGGTGNEGPDQDELL
                     ELKKERLARRYYKSEILYPRKGIEYYIAVTAYDRGIPSNDLNFLETGRDADANMRVFF
                     PGTLAKENMDKIMVVPNPYIGHSSFDGRRENDDKGDKSRRIWFVNLPKRCDIRIYTLA
                     GDLVQTLKHDGAPITDIITISKASTAGVAADGMECWDLLSKHQQIIAPGVYLYSVENK
                     ANGDVKVGKFVIIK"
     gene            26348..27379
                     /locus_tag="PLB65_01020"
     CDS             26348..27379
                     /locus_tag="PLB65_01020"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOQ77136.1"
                     /translation="MKKRMIIIITIALVCVPLLIVAKPFGKTGTVALQFLKLGVDARA
                     VGMGEAYTAVTDDISSVYWNPAGLAPAFENQVFVSHTNWPADIMHEFGAATYTNGVST
                     IALYGSVLHMDDMDVTEEETLGPTGEKFTCSDIALGVAFAQQFTNKFSAGVGVKYLRE
                     NLYEFDINSYAVDLGSMYNTGWRNIKIGMAMRNFGPDIRFRVDDDDDGSYDEDPFDLF
                     DNDGDGLIDEDRIELESKIPLGFSLGISGDIMRQGNNYMIASFQMDNVIDRTETWNMG
                     AEVKLSNLFLRGGYQFNYDTNGFSAGVGWQVPTSLGIFNIDYAYTSMGELAESFIKSA
                     HRVSIKMKY"
     gene            27411..29594
                     /locus_tag="PLB65_01025"
     CDS             27411..29594
                     /locus_tag="PLB65_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77137.1"
                     /translation="MKKALLITLCLTFLVGIAFAQQMDPATNKKALEGPEMKPCILQN
                     SRTVPQYTFTRTPYALLTSYYDYMIGSYNGIPLRVIPSDISDGGYFMTYHARRAANAT
                     RRIFYAYLGADGSLTTNSEISNVTVNEGYPTLAVDPISGKPLYAWHANADSEADYLET
                     QFTADSFIQGTDGVFLPITTLIDNPVTCLTTNDNEYIWPTAQIGPSPIAGKSRVYIVA
                     RNATTHTYGPSENPRIAYADFDGDQIELGEPALDWHFTSIPEMDQWNHDSEWRRPFHA
                     LTTDDAGNVYYAGFHFATESDGSTDIPEPDLDVFKCNNFGEGTWTRVSEWSKMPSWNP
                     NTSPTDTTGVFEISGGNSPYGDDGLYWSVNGSASSHLNATIDDLGRIHYPAIWSLNNI
                     DGYYYPAYQYPKEIIFDPSEAEGSQFLINEIWPKKDPHNNVDQCFQPWDTEEPWGEEE
                     FAQDPDTGQWYVSSYLSWDFPYWDQSAHGDAMFFHCNNMKVTEGNDHGMLATVWQNCY
                     RAKRFNADGDTDYAEWANTPEIWISVSSNNGDNWSEPISLNNIETPEFAGLKPMWVYP
                     ADKIIYVGEQDGLKVGKLGLMFYNDYTWGSFVINPGVGATNDGGQVMFTEIQITFPPT
                     GDAAEDPSVSPVTNMLNQNYPNPFNPETTITFDMPKTANANLSVYNVKGQLVKTLHNG
                     VANYGRNSVVWNGTDNNGSQISSGIYFYRLSTDGKVETRKMMLLK"
     gene            complement(30485..31408)
                     /locus_tag="PLB65_01030"
     CDS             complement(30485..31408)
                     /locus_tag="PLB65_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOQ77138.1"
                     /translation="MKKTLFWILFFLTGLAVIIAYLFIGKPNTNIITQVRIPRLLLTL
                     FTGMALAGIGSIYQLMLANPLAEPYILGISSGAAFGSIVLGITGFIMLMPIGGFIGAG
                     LTMLLVWKLAHHRGRFDKTRLLIAGVIVGMFFASGISLIMYLHQQDTALILGTLMGNL
                     GHIFTKNEWISFLFLFSVSLLILIYLYFKSTAIDILSNGDLYAASLGINVLALRKQIF
                     VLTSIVIGISVAYAGIIGFVGLIIPHIIRFFVPSGQKNIYLLSLWTGGIFLLACDMLA
                     KHLLSMELPVGIITSFIGCPFFMWLLLKRDN"
     gene            complement(31405..32250)
                     /locus_tag="PLB65_01035"
     CDS             complement(31405..32250)
                     /locus_tag="PLB65_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOQ77139.1"
                     /translation="MKKIVYVALIILAMFVFSCKQMNSKAKKGIVVLSPEVAEIICAL
                     DAEEDIIGITAECNYPSSLQQKKVVGSFSVIDKEAIIALNPELVFCSSLEQESVALDL
                     KNLGYSVEVIYPKSIMEMLSAIERIGILIDRKEQATKQISQMKQEILNIKENSIGKTR
                     PKVYLEIYRNPLMSVSDASFIGELIETAGGDNIFPTLERDYARINPEAVIAAKPDIMI
                     CYSQDTLENIINRKGWLNIPAIQNRKIYFEKEINPDLIQRATPRCIQGMKQLASIFDS
                     WRKEQ"
     gene            32949..34451
                     /gene="cls"
                     /locus_tag="PLB65_01040"
     CDS             32949..34451
                     /gene="cls"
                     /locus_tag="PLB65_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HOQ77140.1"
                     /translation="MLIASLTQIIVGGLVLETFSKVTIRIINILFGISIVLIILIVVM
                     ENGSPATTMAWILVLIFLPIIGFILYLFFGRNWRKERLFSKKDYADTQLLATYIQKYP
                     LTIEDNWQTELSARLNKLLKNNSKATLTVNNEVKLYSDTMEAFNAILKGISSAKHFVH
                     MEYFSIFSDETGYDMQKILIQKALEGVEVRFIYDDVGCWMLKKEFKQELHNAGVEFVP
                     FMPVWIPFLNSRINYRNHRKLVIVDGKKAYLGGLNIGDKYLGKNSYYGYWRDSLAEIE
                     GQAVLSLQAIFLADWNFVTGKNLMQETLLDKYLPTDITVKDKFLPMQVVANGPDSDYA
                     SIMQLYFAAITYAKHNIRISTPYLILNETLLSALKIAAISDVKIQIIMPDKPDHFLVF
                     WASRSYFQQLLDVGVEIWTYHKGFNHSKILIVDDEVLLIGTVNMDLRSFSHNFELAAT
                     IYDRNVCAEAIMHFEEDLMNSSKINAEEFKQRSMLYKTRESICRLVSPLL"
     gene            34612..37431
                     /locus_tag="PLB65_01045"
     CDS             34612..37431
                     /locus_tag="PLB65_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HOQ77141.1"
                     /translation="MKFISYPLSRNLDRIVQELAKPNSILVFPTLIAAQRAEREYLRN
                     WDLSRCNFYSVEELRSALILPQLPIVTDEKRLLCLYMVMEESERDFFHIYSYSDIVEW
                     GRRFFDFFEELCVECIEMETLEQLPNSGIMSMQEWQEKYLEKIIAIRNNYFQFISSLG
                     FSDAIFYLKSENIIVPWQGCEVIYINQYYYSALEKKQIEALEDAGNQVTIVTHGLEIS
                     GETDDWKVQDFDLKNAWNSLERKPVIEILETENETQMALSFLSWNMDNNCPNSAIIDS
                     SFHLKSYSHFFPEEHFAKPDNYPFSDGTIYKMLSAIYKGLKASKESDGYLPVRILANY
                     ISEDWFCHYFFEENSNLEREDYIQQLRLELSDIINKDTLYVNCAFFNNINKPVLQSLV
                     LQYNNLMEAFTEATNLSEICSLIDSPNGLCLIKLLQEHEKQYTDIIPVFWTQMANFMA
                     IENLGLINSWKQIFEEDTLGENLLDLLLNYLKSAKISIQRNEKLSPEWEISNLLDSRN
                     RAITKVAFFQMIEGIVPSNPTPVWLFNESQRAKLGLKTYNDVRKWERYYFFRLLLCSE
                     QAICLSYANQERDISPSSFLGELEELLQNEDVSFLQKKMVGVSLTEVYSENTPVKTIP
                     ELENNEYCCVEKLPEEDFFIIPSDPMTDFPKSNIRFSASALIQFMKNPFLWYVENKCK
                     LYTQNWEAEETISYKLFGNIMHSYFSTTLSKLTGRHISTDALEAFFGNSAKLEAQLRE
                     VITSDKLIYKIPKNYNAEFLGDILAKKLAQSLYLFYKDWLKKQVEYRTFDLYPETDDD
                     SNQVVEYKKLGTVSFQERDYLLYIGGRADLRVELDNKAIIVDFKTGSHDYRQLCIYEW
                     YYYLLDEILPADNVSSLFWNILDPTKKTEGVNEEKRQKLKEQIFEHFTYCLANGYSTI
                     TKVSDRQRLQYITRADLLREIRGFE"
     gene            37428..40769
                     /locus_tag="PLB65_01050"
     CDS             37428..40769
                     /locus_tag="PLB65_01050"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOQ77142.1"
                     /translation="MIENNCRIICASAGTGKTYRLSLEYLALILKYYGKSEFSLDNIL
                     VLTFTRKATAEIRERIIKHLELLLSDNVEDMDEQNILLNNLRELMEERFVPLSEIEKN
                     MLLSARKELICDQSHLQVMTIDSYTGQIFRNIVRPICNIEHYDIDTEAIIKIMPLLFK
                     HLMKPIFRESLQDLLSKKVSRSLDDYTSFFRSLIEQRWLYYLITKRKSTRDSSSNGIL
                     MNLDPDADSTEYYGNFLNAMQELIQIVVNICAEKNEASLADYFNKEFKQLVNIESNTI
                     NAIYIMDNLKRLDLDAAEKLLTILDKSNIWNKQKISAKKYTVENTQLQNAQDMAKLFL
                     ADYLILKLYIPEQKQILGIWSDVLKEYDRLIYRYKNLTYNDITWLTFEALFSNEPPFL
                     NPQSADSATEFYQFLTHRTRFLLIDEFQDTSLIQFNVLKPIIEEITAGEGSKPFGGVI
                     VVGDEKQSIFGWRGGERDLLLNLKDIFPALKEVKMESLKQSFRCGPTLMEFINQVFSN
                     SALHQLLQEKEMQWNYARIESQEYKKEEGSEIEFCLRNLSKSADNTNKEKDIYQDFIE
                     CMVMPALRKDETGSIAILCRTNNELTKIQHVLDEYKITSLYQPNSSIIEHHYVAPLLS
                     WLRFQAWGDWMDFIAFLRSDYVLINTKVLKQTLAIIARTLELQKEWEKPILPDFSALP
                     IINTLFQLSLQQQGKKATEICRDLIDFCLPSKQLSERDYLNLQRFLDILATRMLTDPE
                     KGTSFPDLLAYFNENANSENFQQVSVNISDSLQLLTFHKSKGLQFKRVFVFYNFYGKH
                     RDTSDTISWALSYEDKTFNKIRDYGISLHYQKIMKLSSYAPLYLQEQKREQLEEMNNL
                     YVAITRAEQKLHLYFGFQYKEGWDAYYDKYKSESLPVAICNAVQEIFMKSTPDERGVY
                     VQKSMFELCEKDKTEKSERSKAIREQINSSIKICHHRPFDPANYLPAEVPDIKDLKKY
                     YLIERPNLIGDLLHFYMSFIIRNELKEHQYALYRCLNRYGAIFTGSQIDNFAARCKQV
                     CEHNPYLFTTRWNEIFTEQEIVENSKIFRIDRLMINTAEKESLIIDYKSGDIHDPKQL
                     INYKNALLKLPALQNYQIDTKIVPL"
     gene            40995..41261
                     /gene="purS"
                     /locus_tag="PLB65_01055"
     CDS             40995..41261
                     /gene="purS"
                     /locus_tag="PLB65_01055"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HOQ77143.1"
                     /translation="MLKAKIYVKLKASVLDPQGKAVTNSLHNLGFTDVLETRISKYIE
                     MNFTSDDREKVSQEVEYICNSLLANPNTEVYQYEIVPLEQNLEN"
     gene            41251..41937
                     /gene="purQ"
                     /locus_tag="PLB65_01060"
     CDS             41251..41937
                     /gene="purQ"
                     /locus_tag="PLB65_01060"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HOQ77144.1"
                     /translation="MRISVVTFPGSNCDYDAYRVCLSRGHQTKLIWHKDTDLANPDLV
                     ILPGGFSYGDYLRGGALARFSPIVNEVISFAKKGGLLLGICNGFQILTECGLLPGTLL
                     MNESLLFICKHQYIRVETEASPFTQSIGKGKVLDVPIAHKEGNFYTDSETLKQLQDNN
                     QIIFRYCDKQGEINLQSNPNGSLDNIAGICNPQRNILGMMPHPERSATDTVPTQDGKW
                     IFEAIEKALE"
     gene            41918..42616
                     /locus_tag="PLB65_01065"
     CDS             41918..42616
                     /locus_tag="PLB65_01065"
                     /inference="COORDINATES: protein motif:HMM:NF039185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOQ77145.1"
                     /translation="MKKLWNNFLELIVPPACLVCGNRIEDRTQVICEFCEAKLSLIGE
                     GTCPVCGSENKELPCEVCLDGQFEFDSARSIFRYIGPIRELIHILKYEGYTYPAGYFA
                     LPIAELIESEPSLKNHDYICAVPLHRVRKRERGYNQTDLIAYAVSKLICMPYLNPVTR
                     RINTFSQTLLSKEHRIANLSGAFTVKHKDQVTGKKIILIDDVFTTGSTLNEIAKTLRA
                     AGAKKISTITVARA"
     gene            42619..43008
                     /locus_tag="PLB65_01070"
     CDS             42619..43008
                     /locus_tag="PLB65_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77146.1"
                     /translation="MNKEYLEIRPETSESEINEMIEKVAKFIAERHLAPAGIMFFESI
                     RPLHGIGSQALFMILPIAEIIFDSRKYQQFAIMMEDEKNLKKLISRMDELDEEYNRER
                     RKENRLKRQHRKAKRKAFFRKIFKTKK"
     gene            43034..44896
                     /locus_tag="PLB65_01075"
     CDS             43034..44896
                     /locus_tag="PLB65_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HOQ77147.1"
                     /translation="MQYDEKRLTRIVATDCGSTTTKAILIEWVDGEFRQTLRGEAPTT
                     VEAPLNDVTKGVINATQELEELARLKYHNPNIKFMENGEFVIPRKGDVGVDAYVSTSS
                     AGGGLQMMVTGVVAAMTGESAERAALGAGAIVMDLIASNDRRMNYEKIERIRQLRPDM
                     ILMAGGEDGGTVKHVVEMAELVASADPKPRLGSGYKLPVIYAGNKDAREEIIKTLGEK
                     VDLIITDNIRPKLEIENLLPAREKIHDLFMEHVMKQAPGYNKLMEWTIGPEHEQVPIM
                     PTPAAVGNIMQAIAKEEQIEVLGVDIGGATTDIFSVFTKDFVFNRTVSANLGMSYSIS
                     NVLASAGLDNIMRWVPFDINENELRNMIKNKMIRPTTVPSLLEELVLEQAIAKEALRL
                     AFEQHKSFASSLKGMQKQRDISEAFSQSLSGETIVNMLTLDLLVGSGGVLSHAPRRNQ
                     TVMMLIDAFLPEGITRLSVDSIFMMPHLGVLSEISTKAATEVFNKDCMVYLGTCVAPV
                     GKGKIGKPALYAKLELPDGSVFEEDIPFGEIRLIPCEVGQKAKATLKTHKGLILDQDK
                     KKEITVDLHGGIVGIVIDTRGRQPFVLPTEKSERIAALKKWMKELKAYPEHILV"
     gene            44906..46036
                     /locus_tag="PLB65_01080"
     CDS             44906..46036
                     /locus_tag="PLB65_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77148.1"
                     /translation="MGQAYSAGLTVTDNIILRKERILPLKGQVVVKKGDKVKAEDVVA
                     ETLLPGKVIPFNLANKLGVTPQQLENYIKIQPGQKITKNTVLAANKGLFGLGIMKNEV
                     LSPIDGEVENISSVTGQILLREPKIPVQVKAFIDGLVTDVIEDEGVIIENKSAYIQGI
                     FGLGGETYGELKILAESPDDELDVAKIDASCAGKIIVAGAFAAYHSIDIARKNGVKAI
                     ITGGLDDQDIKKLLGYDIGVAITGHENIGITIVCTEGFGKINMANKTFELLKRFEGHK
                     TSIHGRTQIRAGVIRPEIIIPLEFDEKELVAKTSSMPVLEIGTVIRIIRQPHFGQIAK
                     VTALPEELTKVESETLVRVLEAEFEDGTRTILPRANVEVIES"
     gene            complement(46157..46411)
                     /locus_tag="PLB65_01085"
     CDS             complement(46157..46411)
                     /locus_tag="PLB65_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77149.1"
                     /translation="MLQFSLHISMFFLNNSAGDAVELIGNFPISATAIAPQEPSKKQP
                     DFQKWHTRQYLRKEKLLKKVGLIRKNDIPPQVIWGNVKYS"
     gene            46708..48228
                     /gene="proS"
                     /locus_tag="PLB65_01090"
     CDS             46708..48228
                     /gene="proS"
                     /locus_tag="PLB65_01090"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOQ77150.1"
                     /translation="MSKNNKTAITPTREENYAEWYQQVIKAADMAENSEVRGCMVIKP
                     WGYAIWENIQHILDRMFRETGHRNAYFPIFIPKSFFEKEAEHVEGFAKECAIVTHSKL
                     EADEKGGLKLAGELTEPYIVRPTSETIIGASFAKWVNSYRDLPLLINQWANIVRWEMR
                     TRLFLRTTEFLWQEGHTVHETEIEAREETLKMLNVYATFARDYLALPVIQGEKTESEK
                     FPGAVTTYCIEAMMQDRKALQSGTSHFLGKNFAKASEIKFQNRNGEMEYAWTTSWGVS
                     TRLIGALIMAHSDDNGLVLPPKIAPSHIAIIPIYKNDEEKQIVMDFAIKVQNIFKGLY
                     LDDIQIFTEMDTRDMTSSERNWYWIKKGIPLRLEIGPRDIVNDSVMLARRDKEPKDKH
                     SIKIDNLVDYVLKTLSEMQEGYYQRALNFRDKWTVDIDDKEEFYRFFNSKNEEQEISG
                     GFANSHWCGNQTCEEKIKDDLKVTIRCIPFEAKEEEGKCIYCGEKSNRRVIFAKAY"
     gene            48185..49456
                     /locus_tag="PLB65_01095"
     CDS             48185..49456
                     /locus_tag="PLB65_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HOQ77151.1"
                     /translation="MKNPIVELFLLKRIKMNAAIINIGNEILMGKTINTNLAYLGSEL
                     TKLGILAEYAVTIKDEPNAIKQTLQDTWKKYDIVITTGGLGPTEDDLTKAAIANFFGK
                     NQHFDNSIWEHIQKMFAFRNIPIPETNRSQAMVPDDFIPLQNDLGTAPGLLYQEGDYL
                     FFALQGVPSEMKHIFETHIKKIIAKTYPNYKPLHIKTLRTYGIAESRLAELFTLSDLP
                     EGISIAWLPQIGRVDLRFYGTDVRKVEEAADSALNKIHPYVWGYDEESPAEILLSLLL
                     NNFYTLSVAESCTGGLVQKLVTDVPGASNSFLGGVITYTNELKKKILKVSDKVLETKG
                     AVSETCAMQMAEGIKLLTNSTCAISITGIAGPDGGTEKKPVGTVCFGFIAAEKHWSEK
                     QFFTGDREIVRIKAAEFAILTLIKYLQGRYN"
     gene            49453..50700
                     /gene="purD"
                     /locus_tag="PLB65_01100"
     CDS             49453..50700
                     /gene="purD"
                     /locus_tag="PLB65_01100"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOQ77152.1"
                     /translation="MNILVIGSGGREHSLAECFAKSKKVAKVYVSPGNAGIAKYYECV
                     KLPKQEDICNFCRQNTIDLVFIGPEQPIAEGLSDYLWEKGISVIAPSKFAGQLETSKI
                     FAKDLMQKSKIPTARYQIARTVEQALELIENFGYPAVIKADGLAGGKGVIIVSDLNEA
                     EDALNKITISARGVIVEEFLKGWEVSLFAFTDGVNFKTTLFAQDHKQLYDGDRGPNTG
                     GMGAYAPVPEAEKYRSKIEKEIVEPTLKAMRDMGYPYKGILYCGLMITREGPKIVEYN
                     CRLGDPEAQVILPLLETDLVDICQAILQSEINKIELKFSDKTALGVVLASGGYPLSYQ
                     KGFPLIMPSTLSGIYFSGVADAENGLVTSGGRVLCAVGVGKDLNEAREEAYSKAQQIT
                     FENRIFRQDIGLRNNTLLDNGDK"
     gene            50705..55786
                     /locus_tag="PLB65_01105"
     CDS             50705..55786
                     /locus_tag="PLB65_01105"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ77153.1"
                     /translation="MTIYSIIQVKNLSLAKFYKAGLLFYLSFFLPALIFASPITLISE
                     SQDLLTIQFELPEYELEDVTINGQTWKRIICSESSIYGEEGYPELKLFSTAIAVPVDG
                     DISFSIESSESQTLKNVNIYPAATMLLEESEPIFVNKPNYKAYSNRELYPLQVVQKGE
                     PAFVGNRRFVPLFICPFQYRAQSKELIIHRSITVSVYVAGSKNASKNWQLNPNPLDNA
                     TPSLFLNENSSKIWRLEKTRDTNYTSPKNGTSGINEIQIIVDKEGIYKVDYHYLMDYI
                     NMMVDSLQVNMNWTPANVDPRYLELSDEYGQVPINFVGENDGIFNPNDYFEFYGDMHK
                     GDTSYMDDYTSENVYTLKLVDRFGARMAVENGGLIESNISNCIVPDAYEDTVHFEEQL
                     ISDKLGQGWSSIDQNYYREDVWFWRKIDAPDLEIVPIELQYPKDTTTRYASAKICLMG
                     LTYSETLTSGQFDHEASIRLNQAMINTLTWIGQTEKIFQNQTEISNTALRHGVNNLYI
                     ALSGNTVMGDEEQVLLDWAEIKYWREYKTDQDYIKFTKPSNLPDGLYQFEVGGFSNAD
                     ISVYKIGSSIFNSVQIEPFNINGDAPWTVTLQDSVASDAVRYFAVTENKKKTPKNIRL
                     NFPSDLKNPYLSADVVVITPRKFTDVEGTLQLKSLWESEGHTVQIVDVQDIYDEFNFG
                     ITGSEPIRDFIRYAYNNWSSPQINQVILLGEGVDDTRDNSYSRMYNLIPVKKTWTYNH
                     GATASDQWYGCIVGTDNIPDVSIARISVWKEEQILAYAAKAISYRENLQTSRLWSSHL
                     TFTSGGKITDGNDLFAQQSERIIRKTIPPEYRVTRVYTSTQTVSSDYFGGTFNLKDAI
                     NSGTQYVQFMGHGGGRIWADYNLFNFNDVATLNNQVYPVVLSLACYCSAFDTNGMASI
                     SEALVLQPGKGAIGTAGFTGLGFRDHDEDWGLAYCEALFKHNFDNIGLANIYALARFY
                     STTYSPAARYALINGFAYMGDPLIKLRKPIKDIPVSANSYVLNPGDTLYVNADFPSGI
                     NAARLFIMKPSGKIVNVPYDLPVYPDGHFTASYVNPDQTTDNYFRKIMIAGYSPSDEF
                     VGISQYSVGRPNIVHHSFIPAEPAWSDSVLFTAKVFSPLPVQNIYCKVRTDSTSQVIN
                     WVDLPMQRSETDSTLYVTATRLGRQPTRKEIFYKYVLETEAGVSETILQSYVVAGPDL
                     LLKDIKLEQDGNNLVLKVLGTNVGNTPSITTDLKLYVGTTANNLAFFSSQSYPPLEVN
                     ELRWDSISLANLPNANLFLEVRVNTSNTFPEWEAYVNIGNYIRLQVPLNYYLIDSSGS
                     TLSSIDNNLQCTIPEGLVPTNEQILFAVNTLSALPPLNQPDVQNIMMNAPDGFSGNKY
                     SIPYEILALGTGVTDSLGVLNGGKKLFLSFNYSAIDSLTQAQENANNFRIYRYNSQVK
                     KWILIGGFVDVNLNKVQFEVDRTGIYSIFRNIDSSPPSIDVNVQDQEFTVGGYVAGNG
                     IISLLLSDANGIDVIDNSIQLFLNGIPIPEEDYVVSINLENINRIPIKYQLSLGRGIH
                     ELKVKCRDLNGMLATRDIQFTVNDKFDVINLANYPNPVLGTGGQGAAIDPKNEGRTRF
                     TYILTDGADEVTIKVYTISGRLVKTFRNLPVGVGYHEYPRTVYGWDCKDDSGYTLANG
                     VYFYRIIAKKGHKTIEKTQKMAILN"
     gene            55797..56807
                     /locus_tag="PLB65_01110"
     CDS             55797..56807
                     /locus_tag="PLB65_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77154.1"
                     /translation="MKKISILFSLTLMCLTPLFAGRYAGDFMMIGSGVRPLGMGGAFS
                     SLADDGSAIYWNPAGLCFITDSEVSAMHCFLYNNLASYDYVTYAQPLPNDVTICLNVT
                     RLTVSDIPHFDERYLIGTNVDQRINNSVYHLPGVPDSKFSSYDDLYQFAFSKKIHYDA
                     NMGWEFFAVPFDFGFGGTVKLIKRSIWDRTGTGTGIDIGFKAKTDLAVIFDWEDLGNL
                     DFGINFQDIGGTDITWDTPSKIRDEVLYNTKTGVAIEQPIPKLKSIVTLAYDYDYVYG
                     GTNHYGVDWEYDKKCNLRLGYYDTNFSAGATIKLYGVLLDYALITNPIGISNRLGLRV
                     RF"
     gene            56819..57604
                     /locus_tag="PLB65_01115"
     CDS             56819..57604
                     /locus_tag="PLB65_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77155.1"
                     /translation="MRKICILAGLLLLVLTYSCAADDSPEKDKIPPAAPDLLEHLGDT
                     GDDPITIDGIEVILDDDNNGIDAVPDGKWIKVPWKKFVDNDLSHVKVYRYTVQNPEPL
                     LIDTVPASEDYYLDRSFLIEREWYYYYIELYDASGNFSVSDTVSYALLAKSTLTSPEG
                     GAAVNPNNLRLYWRIGDSQTTEFRILIWDEHTGELVYHTDYSYTPNDENPAPPNVPFP
                     VMTPAPVNGQVFRWRVDSFDYDSEHEMKMGSESEERTFIIQYK"
     gene            57700..58182
                     /locus_tag="PLB65_01120"
     CDS             57700..58182
                     /locus_tag="PLB65_01120"
                     /inference="COORDINATES: protein motif:HMM:NF022007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOQ77156.1"
                     /translation="MKKTLLVFLLAGFAFMLFGQQTINFTPSSNISAYTYEGIRSGVE
                     KLKMNNYILGQVYKPGLYTVPDDTDFLTLLSLAGGPREDAKLSKIRVVRPSSEGDKVI
                     WVNFKKYLETGDATLIPQLKPGDTIIVSGTIFYAVSRVASFLSDLTVALSMYSIITGF
                     "
     gene            58195..60483
                     /locus_tag="PLB65_01125"
     CDS             58195..60483
                     /locus_tag="PLB65_01125"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HOQ77157.1"
                     /translation="MLENENNQVPVQEEIKLSDYFRIILQYRYLVILVFFLVIVATVL
                     YTARLPKVYSASSRILLEDANKQSDLMFLSAGGLGKNTVNNQIELIRSKPIMKLAWEI
                     MKKYPDWDTFPASQLPDPVASLNKMKVESKRETDVLTISFESTNPAEAMAAVNSIAEA
                     IQQQNTQYARLEFTTIREFLETQLDAISRRLQSSENDLREFKNLNQLTQLTEETTKLI
                     EKASDVEAEYEAALTEQAVKAKTLDMLNRQLREQDSLLVNIDNILKTPYINELRKQIV
                     DTQSLITKLVTKNEYPLDHPQIQLLYREIENAKETLDREIRKLVSLSINEDPLKTRSD
                     LLTRIIQANVDLEMARAKVDGLEQTKEMYNQRLIAIPNTELELARLTRNLMLDEKIHS
                     MMMEKYEDAKIAEQAKIGNIRIIDYAERPTIPIKPRVSMNILVGIILGLGLGIGSAFL
                     VHSLDTKLRTLEDMESFVRLPIAGTIPVIREAESKLQEFNDMINSATGENREQLTRSM
                     HYVMMQLVSHYAPKSPIAEAYRTLRTNILAKKGEGSVAILVTSSGPKEGKSTSIANLA
                     ITLAQMNSKVILVDMDLRRPTVHTKLGLEKENGTSDFLIDPEVSVEQVIKPSGIANLD
                     VITSGFIPPNPSELIASPRTDFLIEELKTRYDFVLFDSPPLIAVTDALILTKKVDMTY
                     LVVRCGFTDKGIIRRTKELMENIDGRIDGIIVNGIYVQKYYSKQNYYYYYYYYYYYYG
                     DEVPQKQKKNVNRFLRKNKSVS"
     gene            60443..61276
                     /gene="ispE"
                     /locus_tag="PLB65_01130"
     CDS             60443..61276
                     /gene="ispE"
                     /locus_tag="PLB65_01130"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOQ77158.1"
                     /translation="MLTASCAKINLFLELTGKLPNNYHQVDTVLCSIDLFDYLDYEVI
                     DSPQIVLSCNINQLANENNLIYKVASYLQNEYKIPNGISIKLKKSIPIAAGLGGGSSN
                     AANCILALNKLWQLHLSKAEMHKIAAQFGSDINFFLEGGTARGENRGEIITKMPDIIL
                     DNILLINPGIAISSAEAYKLAYLTSKQEQHYFDIKNVYSSCFNRLEAGIRKAYPIVDE
                     VINTLKQYEAKVSMLSGSGSTCFGIFENIAQLEKCKDYFSEKGFWTYRAKTINSENSI
                     S"
     gene            62364..63431
                     /gene="ispG"
                     /locus_tag="PLB65_01135"
                     /gene_synonym="gcpE"
     CDS             62364..63431
                     /gene="ispG"
                     /locus_tag="PLB65_01135"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOQ77159.1"
                     /translation="MDNITRKTTRRIMLGDIPIGGNAPISIQSMLSIKTSNIPEATTQ
                     IRELISAGCEIIRCAVMDISDANAIKELKKISKAPLVADIHFDYRLAIASIENGIDGL
                     RINPGNIGKIEGVQKLVAAAKERNIPIRIGVNSGSLPKDLVQKYSVSAEAMVESALRH
                     IKILEDLAFYDIKVSAKASSIPLMLESYRKLSKACDYPLHLGITEAGTTLSGSIKSAI
                     ALGILLEEGIGDTIRVSLTANPVQEVIVAKEILRNLGLRKGLNIISCPTCGRTRIDLI
                     GLTEQVEKALKNYSDYPLTIAVMGCAVNGPGEAKEADFGIAGGIKEGLLFAKGEIVKK
                     VPEELLVKELLALIQNHIGKQ"
     gene            63434..63967
                     /locus_tag="PLB65_01140"
     CDS             63434..63967
                     /locus_tag="PLB65_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOQ77160.1"
                     /translation="MLLKVFFTFFKIGAFTIGGAYAMIPLIKREVCQKNKWLSEDEFL
                     DGLAAAQSCPGPIAINISIYVGYHLAKGKGMAVAVIGTILPSFITILIIAMLFQQFSE
                     IVIVRKAFHSLRAAVVALIAVPLLQMSRQAGLKLSNFWFPLAIAILVSFFAVSPIYLI
                     IITIAFSIYQSIRKQKI"
     gene            63964..64482
                     /locus_tag="PLB65_01145"
     CDS             63964..64482
                     /locus_tag="PLB65_01145"
                     /inference="COORDINATES: protein motif:HMM:NF014471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOQ77161.1"
                     /translation="MIYLKLFWTFFKIGLFSFGGGYAILAMIRQEVVLKNAWISSSQF
                     MDVVAISQMTPGPIAINAATFIGYQKGGLWGAVLSTLGVIFPSLIIMTLITITYIKLK
                     NQPWFKNIFQKLRLLSLGLIAAALIMVLGDAFRDLFSILVFAGSFLVSWRFKLNPFYL
                     LMGAAVIGVIFG"
     gene            complement(64524..66983)
                     /locus_tag="PLB65_01150"
     CDS             complement(64524..66983)
                     /locus_tag="PLB65_01150"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOQ77162.1"
                     /translation="MMNDDFCTKLSQLSINGTVEEIVLRFMEHLKFDLGKDQFSAAPY
                     DCYISFASAVKDILLDRWLITQPQEYALQRKRVYYLSLEYLIGRSLTNSILNLDIQQQ
                     SIKALTEIGFDLSMLSELEWDAGLGNGGLGRLAACFLDSMASLQIPAYGYGIRYEYGI
                     FFQHIINGEQVETPDNWLRYGSVWEIPRPTRLFPVNFGGTVREIINEDGTKKKHWQPE
                     VTVMAMAYDYLIPGFQNSYVNTLRLWSAKSSREFDLRYFNEGDYVQAVAEKNNGEMIS
                     KVLYPNDNKMQGKELRLKQEYFCVCATLQDILRRFFKKNKDLRNLPDKAAIQLNDTHP
                     CIAVPELMRILVDEKNIPWETAWDITQKCLSYTNHTILPEALEKWQVQLFEKMLPRHL
                     QIIYEINHRFLSDIQISRKVDDDFLRRVSLIEEEPVKSVRMANLAIVGTHSINGVSEL
                     HTELLKHQVFSDFYSLMPDKFNNKTNGITPRRWLMLCNPLLCHLITKAIGTEWKTDLE
                     QLSDLNKYKNDSSFLEDLAEVKYQNKLDFCKYYEDMHDRRFNPDTIFDFHAKRIHEYK
                     RQLLNALGIIYLINQIRDGKELTPQSFFFAGKAAPGYFTAKLIIRFICALGDYIEKDK
                     QLSSYLNVCFLPNYRVTLAERIIPAAEISRQISLAGTEASGTGNMKFALNGALTIGTL
                     DGANIEIREAVGEDNFFLFGMTAAEVHYLKEQGYHPYEMMRQNEDIKRIFDFIESDEL
                     NPLNPGLFTPLTDLLLYQGDSYCLIADLQDFIRVNKEATETYKDKQKWNRMVLANIAN
                     MGKFSSDNTILAYAKDIWGVK"
     gene            67195..69468
                     /locus_tag="PLB65_01155"
     CDS             67195..69468
                     /locus_tag="PLB65_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HOQ77163.1"
                     /translation="MKLMSNYQPKLMVIIAVFYLFCFGIRAAVVQPEIALLAAKNYLQ
                     ETGLSKSVNTDDYQIFTYNSAGKLASLPHIYVQNENPVLYYIQYADGNFAVVSADDNF
                     YPVLAYSNEGLTRIDNLPSAFYYWLDTYSAQIQLIREKNALYPENSQLWQSLLEGTYT
                     NLSRTDRSVGPLLTTNWDQSWPYNALCPADQHGPGGHVYAGCVATAMGMVMKYWDHPV
                     SGVGNESYYCPGYGYQSANFETTTYLWDQMPNSISSNYLPVATLLYHCGVAVHMGYSA
                     DGSGAQSTDAAEALVDHFRYPTAQYESKDSYSNSEWNNLMTAQIDNGTPVYYSGYSNE
                     GGHAFVLDGYDTANHFHFNFGWSGSGNGYFYTTDVNGFSTWQGAIINTIPENYNINTI
                     PVVLNANNAEAGDNFTLEIKTKPVLGNWNVNHYDIMLYYDNENLDYTGYSVQGTLSQE
                     GNITIVENSPGNLSIDWNSNTSLCGGGVIIKLNFVARDAGEFLFDILSMHYNTLPVNN
                     VVFTMVNITAPVATLAESNITLSNIMHLVYNSIGFTQVNTSYLLPSWNVNHYQFNLNY
                     NPAKLEYAGYDVNGTLSDGCEINVDSSTPGVINISCDTESKFVGSGILIKPQFRAIGN
                     TSTISITQISLSDFYYNDTAVLGSGSANVVLSPYTAIDDEVISTPKPKLEIYPNPFSG
                     NTTIKFTGTAKGAVTINVYNLKGQFVKELTISDFSQREIQWNATDSKGRNVSEGIYFM
                     QWQQGNLRGTDKVLIIK"
     gene            complement(69667..70275)
                     /locus_tag="PLB65_01160"
     CDS             complement(69667..70275)
                     /locus_tag="PLB65_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOQ77164.1"
                     /translation="MVKIFYYLERKLGALLLRLLRKTLKFEVINQETSDRIRCIYMFW
                     HRNLLLMTLQRIDQGAAVMVSSSKDGEMIAGPLSELGYIPVRGSSSRKGSMAMREMIR
                     MSKTISLAITPDGPKGPCYTFHPGLFQIAYLAKIPIVAVAVNANKEWLFNSWDKFRFP
                     KPFAKLTMIYSDPIWVNNKEDIAIVEETIRSFLEEKETEFHK"
     gene            complement(70268..71413)
                     /gene="lpxB"
                     /locus_tag="PLB65_01165"
     CDS             complement(70268..71413)
                     /gene="lpxB"
                     /locus_tag="PLB65_01165"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOQ77165.1"
                     /translation="MTRKESYKIFWLVGESSADLHSELVMKALNDKFGNLTHIGIGGP
                     RMQRQGLKPLFPFERFAVMGFVEVVKHLFFFLKVEHKLSKLFATEEIDLAILVDYPGL
                     NLRVAKMADELRIPVLYFICPQFWAWKHKRVYQLRDSVRYVACILPFEEEKLSIHNIN
                     CSYVGHPIAEEVNIELSRDAFAQFFGIDPNKKWLGFFPGSRNNEIAKMLPTFLQASEK
                     WDPNEYELLFSKSHSVNHQLYMSYIEGKKHITLIDGYTYEMMKYCDLLICTSGTVTLE
                     AAFIGTPLLICYKANFTSYLIGRHLVKIKRIGLPNIILDNDILPELIQKEMNPSNIYK
                     IGMQILNDPEKNEEIRKQLYKLKALLSDKHPSVEMPKIVRHLLDTYG"
     gene            complement(71410..71976)
                     /locus_tag="PLB65_01170"
     CDS             complement(71410..71976)
                     /locus_tag="PLB65_01170"
                     /inference="COORDINATES: protein motif:HMM:NF018410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HOQ77166.1"
                     /translation="MNKYLKPLLLMILIVVLSQPLFGNSTADKTVAWLESKNVSPELI
                     VLIISMIPVIELRGSIPVAIFLFNFHWLKAALLSIIGNMIPIPFILLLVDWFFSLISK
                     VKPGKKFADWIFARTRRKGKSIENYEAIGLTIFVGIPLPGTGGWTGALAANIFGIRFW
                     RAMFCILLGVILAAIIVTILSLMGTLAV"
     gene            72606..73211
                     /locus_tag="PLB65_01175"
     CDS             72606..73211
                     /locus_tag="PLB65_01175"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HOQ77167.1"
                     /translation="MNDQLQELLKKVYEEGVAKANAEAEKIINSAKEQAEDTVSQAKQ
                     KAETILKEAEKKAADLKKNTESDLNMAYNHTLTALKQKITDLILSEALNTKLNAAFNE
                     TEFVKKLIFETLTAWKENASSGTIMISEKMKPLLDDFYLKSLKDIFDGKLKVDFSPTM
                     KQGFIIAPEDGTYKIKFESDDFANLFKSYLRPRTKEILFGK"
     gene            73218..74105
                     /locus_tag="PLB65_01180"
     CDS             73218..74105
                     /locus_tag="PLB65_01180"
                     /inference="COORDINATES: protein motif:HMM:NF022415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2764 family protein"
                     /protein_id="HOQ77168.1"
                     /translation="MKQEYYYFVSGLPNIIIDDTKLPWTPEEFLAEAKNQVSESDYRL
                     LEILHLPMDMDNLLRVLYKSNKEHNNEGLYEDTFWEEYLVFLRAVVENPELKTPVEFA
                     ALPSFIKEIIVKVLNQEEMQILPQTELELLKQFYAWTAESPNDFIRDWFAFDANLRNI
                     LTAINGRKFNLPYAQYLIGDSETVERLSKSHAADFGLGKDNDLFNALIRIYEQNNILY
                     RERGYDIVRWKWIDDYNFFNYFNIDRILGYYCQLRILARWIKSSPELGKEVFNSILSD
                     LNNSFSFPADFNIRSTQRK"
     gene            74119..75873
                     /locus_tag="PLB65_01185"
     CDS             74119..75873
                     /locus_tag="PLB65_01185"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HOQ77169.1"
                     /translation="MTTGIVKGIISNLVQVEVSGPVSQNEICYIIQGGVKLMAEVIKV
                     VGNVANTQVFESTRGLKPGDQVEFTNHMLEVKLGPGMLSKNFDGLQGDLNKREGVYLT
                     RGEYTDPLDEDSLWKFTPIAKAGDKVSAGDWLGSVPENWVNHKIMVPFVMEDEYTVKS
                     VVPEGEYKITDTIAVLTDNDGKDIPVNMIQKWPVKIPITAYKEKPRPFKLLETGVRTM
                     DTLNPMVEGGTGFIPGPFGAGKTVLQHSISKNAEADMIIMAACGERANEVVELFVEFP
                     ELDDPRTGRKLMERTIIVCNTSNMPVAAREASVYTAMTIAEYYRNMGLKVLLLADSTS
                     RWAQALREMSNRMEELPGPDAFPMDLPAIISNFYARAGFVYLNNGATGSITFIGTVSP
                     AGGNLKEPVTESTKKAARCFYALSQARADSKRYPAVDPIDSYSKYLEYPEVIEYLNTN
                     VNPHWVVDVNHTKDIILRGKEAQEQINILGDDGVPLGYHDKYWKSELIDRVILQQDGF
                     DKVDQSTPMNRQQYMLELILEICNTNFVFESFEEVMPYFTRLINICKQMNYTEFQTAE
                     FNNYEAELRKIVEERRAK"
     gene            75873..77195
                     /locus_tag="PLB65_01190"
     CDS             75873..77195
                     /locus_tag="PLB65_01190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HOQ77170.1"
                     /translation="METQAFQKIYTKLTQITKATCSVQATGVGNEELATVAGRLAQVV
                     KIVGDNVTLQIFAGTEGIGTDAEVVFFGKAPTLKVSDELGGRFFNAYGEPIDGGPEIE
                     GKEVEIGGPSVNPVRRKQPSELIATGIAGIDLNNTLVTGQKIPFFADPDQPYNEVMAM
                     VALRAQSDKIILGGMGLSNDDYLLYKNTFENAGVIDRIVSFVNTTENPTVERLLVPDM
                     ALTAAEYFALEKHEKVLVLLTDMTLYCDALSIVSNRMDQIPSKDSMPGSLYSDLAKLY
                     EKAVQFPDGGSITIIAVTTLSGGDITHAIPDNTGYITEGQLFLKRDTDIGKVIVDPFR
                     SLSRLKQLVIGKKTREDHPQVMNTAVRLYADAANAKTKLENGFDLSDYDIRVLDFAKE
                     YSEQILAIDVNISTDEMLDRTWALFQKYFNRAEIGIKDEFMSLYWKKE"
     gene            77192..77791
                     /locus_tag="PLB65_01195"
     CDS             77192..77791
                     /locus_tag="PLB65_01195"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HOQ77171.1"
                     /translation="MNLKFQYNKISQLQLIKQLAVRQRALPTLKNKESALRLEVKKAR
                     DNALALDQKVKERTAELDAFMKLFVEFDPDIVKIKNVEIKTRKIAGVKTPLLENIEFE
                     IADYNLFKAPSWFPEGISLLKEICQLQIERDFFIRKMHILEQVRKKTTQKVNLYEKVQ
                     IPAFEDAILKIKRYLEDEENLSKASQKILKSRFESETRK"
     gene            77788..79572
                     /locus_tag="PLB65_01200"
     CDS             77788..79572
                     /locus_tag="PLB65_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HOQ77172.1"
                     /translation="MIEKMRKYTFVLYHQEYESFLVELQKLGLVHIIRSRDDKTESQT
                     KNLELMQEYGECIKFLTKLKSIAPKQTNPLPTKALLNRINEAREEKEKLQHSIDMVKK
                     QIRDLTPWGHFDYNLVHKLKEAGLTIRFHTCLKNHFKPEWQENYPVKIINEVGGIVYF
                     IVITDDGDPCLEADTFSFHKHTLQELEDHLLELQNKQRDIDEYLYSIAPTAIEMFNEE
                     LKRLSNEYEFEDAMQQGEFEAENTIRLISGWIPVSLEQGLKDFLEKNNVIYFAEEAKA
                     EENPPIKLKNNWFARLFEPISKMYMLPYYNEFDLTPFFAPFFALFFGFCNADIGYGIV
                     IIILAVILKLKLKNKGMKDIMSLLIIFGASSIIMGWVMGSALAYDLKTIPALADKILI
                     KDNNQIFNYALLLGAIQILFGIAISAVKTIRNNGIKFGLSTIGTFLLLLGLTVLGSSL
                     LGTDISKVQPYLKYPIYIGLAMILLFNSPGKNIILNILNGLWLLYQIITGYFGDILSY
                     IRLFALCVSSAILGFVMNSIGSQMAGIPVVGPIIFILFMLFGHTLNIGLGGLSGFVHP
                     LRLTFVEFYKNAAFAGPGQEYKPFGKKE"
     gene            79587..80102
                     /locus_tag="PLB65_01205"
     CDS             79587..80102
                     /locus_tag="PLB65_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HOQ77173.1"
                     /translation="MPDSLQTVAQPIVATMASQNLAYFLAWIGLFLMVALSGVGSAWG
                     TVIGGRATVGAMKKRDDVFPSCMILSALPGTQGLYGFGSFFILKAYITPQINMLQACA
                     ILGAGLMSGTVELISAYHQAKIVATGIESIGSGHDVFAKTLILGVFPELYAIIAFAAS
                     FLIGGIIPTLA"
     gene            complement(80173..81045)
                     /locus_tag="PLB65_01210"
     CDS             complement(80173..81045)
                     /locus_tag="PLB65_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77174.1"
                     /translation="MKASTAIMIAILAILTIVFAVLWINSNDKTKELNQTNEELKKLY
                     ESSTSTIGEIQSSLEALDKDLSGQLFTQKEIPGTSPEERRTQIISSIANMRDQIEADK
                     KKIASLEKQLATSKSQLKGVQEIVNKLKVSISEKEQIMDELQQRLGILSETLESERRT
                     SAEEIQKREMTISEKEQQLNQQSIEANTIYYVYGTRKELIEKGIMDRKGGLLGIGRVS
                     VVKQNISVDQFTSMNLLESQQIRFPVTKKGYSLLTNHPAASYKVEKEDNLNVLTILDP
                     SNFRKQKFVVIELL"
     gene            81131..82531
                     /locus_tag="PLB65_01215"
     CDS             81131..82531
                     /locus_tag="PLB65_01215"
                     /inference="COORDINATES: protein motif:HMM:NF017633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HOQ77175.1"
                     /translation="MKYSYLKVWTDEMADYQSGIESIILYPELLVLKTKDKNALCIVL
                     SRRDAFIFLQDNFASASQGKVIWQKLANCHLTNITLSSTDRIVYLELQHQDIYQQNIK
                     FVIIVELMPPQPNAILVDSEMKIIDAIHKYSYADNPQRQILPGLIYTPPKTSFQPILE
                     EVQNPFPDGSVTCNSYFIQLYYNKLKQEEEEEQRQKLCIALKKELQKLQKKEKILKQD
                     LENAEKADFWLACAEGLKPNLHNIKTGMPSFTTVNYLDSNLSQIEIPLQPDKNPKENL
                     QLYIKKYHKAKKGLQRIKENLEKTKEEIAKIIDLIAKAEKGELIDSGITSSNKPKNII
                     SEAILADKLLKYKIDDEFQIIIGRRAKENDYITTQLAKPYDYWFHCRIYHGAHILLRC
                     LKKTEPRPQIIELCCNLAAWYSKAKFSANVPVDYTQIRYVRKPRKSPPGFVTYTNYKS
                     VYAKPMSLREIKEILS"
     gene            82528..83283
                     /gene="recO"
                     /locus_tag="PLB65_01220"
     CDS             82528..83283
                     /gene="recO"
                     /locus_tag="PLB65_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOQ77176.1"
                     /translation="MKNKLRAILVKQSKYSESSLILQFFSREFGHIGVIAKGILKKNE
                     KQQLLTLCEYELIAYEPKEQGLWLYSEANLISNFSVYPNSLSWATAETGVELINQLIL
                     AQEDITTIYDLTIAYLKYLQQVKDNAVLIFWRFLNRITILSGIGNPLTNCGICKKAIS
                     TPSAYLKSKGELICEECLNDINISSDLIILSPLGQNILALLPEIGNHLQEIKLNRAVI
                     NEINSFFELYWQVHHKQPLHLKGLSVLAQFYDS"
     gene            complement(84783..85571)
                     /locus_tag="PLB65_01225"
     CDS             complement(84783..85571)
                     /locus_tag="PLB65_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77177.1"
                     /translation="MNKKILIAVKTYPNPSKKYNETVCTAGLDEDKNWIRIYPITFRA
                     RPLEEQYKKYQWIQVDIQKTTGTDFRPESYHLSNLDDEIKILEEVSSYDVKKDYLLHN
                     VYHSLDDLLKDSNNPKYISLATYKPSSIISANYQPCSRNWDAKDQEQFRQLNIFYDSP
                     KNPLKKIPYTFKYTFLDINGSEHNMSILDWEIGALYWNCLKSTKSEKEACHKVIEKLE
                     YLANKRDLYFILGTTLEHQKRKFHNPFTIVSLFYPPFDDQLTIF"
     gene            complement(85568..86437)
                     /locus_tag="PLB65_01230"
     CDS             complement(85568..86437)
                     /locus_tag="PLB65_01230"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOQ77178.1"
                     /translation="MLYKRQQEVRYLVSKLNNSGKVKLQKLMFLVSQKQETYSYDFVP
                     YKYGPYSFILQKDLDYLVRNEFLYFKQNHYQINNADAIDITSQRKKVLNDIISRYGFF
                     TTQELMQYVYREYPKFAIKSCKADSLLSAAEMNNVQKEIPNNEKPALFTIGYEGRSID
                     KYLSYLVFAGIKILIDVRANAISMKPEFSGKNLSNFCNLMDIEYVHIPELGISSEHRK
                     KIKDKEILFKNYKGVLETNYSDYLDQIVQLALKNKRIALTCFEANPAECHRSIVAKKV
                     FKRLSAEFTLKNL"
     gene            complement(86602..87744)
                     /locus_tag="PLB65_01235"
     CDS             complement(86602..87744)
                     /locus_tag="PLB65_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77179.1"
                     /translation="MVSSDDLIRFAAFKLPFENSLYTENFLTIEETQKRIDYDDLRVN
                     AIPRKKLLEEGYNNNVENKYQGSKWGSIYIKAPDIYFTIMEKAKNKLIKLGDVAKIKF
                     GVKTGADEFFYIPKSNPFKIEKEFLKPVIKSPRECKSILIDPDKLKYWIFMCPYEKHQ
                     IQGTNALKYIEMGESAKKYIKQGKDKGKYIIGINNVSSVKGRKNWYDLGVRELPELIT
                     PSSFSDTFKVYLNNGVLANKRLYEIHYKDEVNVLSAILNSTIFFFYLELNATNSLGEG
                     LLDKTVFEFESTPIINPQLLSAHTKVILQLDSLKNRKILSIFKELQVDCNNYDFRNAN
                     PLPDRKLLDNVVFSILGLSNSEIQEVYAGLLELTSSRLKKANSLKL"
BASE COUNT        28322 a        15123 c        16951 g        27491 t
ORIGIN      
        1 tgaactttga actaagaact cctgcacttt gaactaagaa tagttcacgc agattgcgca
       61 gatcacgcag atttagtttt agaaacgagg atttaaggat tagtttgcgc agatgtcaca
      121 gatgaacaca gatcttgtat tagaaatcgt tttgtaatga gggttatcct tgctatatgt
      181 ttacttttcg attttttcgt tatagtttta aggggatata gagctcgtaa aaatcctttc
      241 gattgtctat tattttaact ttctgccttt gttcaatgta ccattgattc aaatgatctt
      301 cctgcatttt tttttcggca gcagcaataa tattttgttt atctttttcc cagatagtta
      361 aatccggcaa ctggcgatta gttacataag ccaaatacca gcgtttttcg ttttcgatta
      421 agggtgtaaa tgaacccaag ggagtgttta aaattgcttc attcaaggca ggaattttgc
      481 caatggttct aatatcgcta tcagccagca atttatccac ttccacaata gcaatgctat
      541 ctctggcggc tgcctccaaa tatgtttcag gagtttctct tttcataaat tcctgaacat
      601 aattatccat agtgaacatt cttttagtgc ggttggcacg gatgatcatt tcctgctttt
      661 taatttcgaa atctatatag tattccggaa tttcgctatt tagttccaga acaaagatgt
      721 cacctgttgg tgcgtaaaaa atatcgggta ttgaacctac tggattattg aaggcaaaag
      781 caatcaaatc tggagctcgt ccgatcaaag ggattatttg gtcttgtgga ttaaaatagg
      841 ggctttcttc caacggcaaa ttcatttctt ttgctgctcg ttttaagcca acttctttag
      901 ctcgattaaa taaagtagtg ctaagtgttt tattatcttg agctatcttt tccatatctt
      961 ctgatttact tagagctcca gtaaaacgca caaataggaa acggcgtcca ttttcgcgtt
     1021 taaattcctc tttatgagct tcgtaatact ctctggcttc ttcatcagtt acatttatat
     1081 tggttagttt attataatca aaacagatga ttttagcact ggcatagtta ttttggtgca
     1141 tccaggcatt tttaacgctg tccggatttg ccttgacctc atttttaatg gtttttatca
     1201 atttattgta agaatagagg tcacgagcat aattcatcaa ttccttttta aattcagggt
     1261 ggtcttttaa tgcttgtaaa tattttgcgt tgtcgaactt tccattggtt tggaagtcct
     1321 ttatttgttt aacctcagaa gggatatttt tctttatttc cgcttccaat tcggcatcgg
     1381 ttacctttat tttgcccgct ttgatggcct gatcgtaaac atagatacct acaagttctt
     1441 cccaatattg attatttagc ttgatgctat cctgtttggt gagtactttt ccccctccgt
     1501 aatattgcag ataggcctta aaaccgtcat taaacttttt ataattatat tccctatctc
     1561 caacttggcc aactaatgca gttggatcgg gcttagtagc cgctaaagca attgaccata
     1621 ataggcagat tacaacaagg atcgctattt tatgtttcat tcaatcactc ctcataattt
     1681 gggacttccc ttttcttata aacaatactt ttgaatagcg gatttactgc aaggaaaaaa
     1741 acagagaaag atattaaatt aagcagaaga agataggtta tcattactat tgtatggaag
     1801 ttatgtttgt gatttgctgg aatagattgc aaaaatgaaa ccccgctttt agaggcgggg
     1861 tttcgcattg taattggtta cagtatataa ctgtagcaga gctttatatc aaactccgca
     1921 acaaggttat ttagagagta gcattttacg ggtaatattc ttaccaacat tggttacacg
     1981 gtagaaatat aatccgctag cgactgtttg attgttatca tccaatccgt tccaaacaac
     2041 tttctgattg ccagcagtca tattgccatt tactaaagtc cgaatcaatt gtccttttaa
     2101 gttatagacc tttacgctgg taggtccgct ttcaggtaaa ctgaagtgga tagttgtttc
     2161 aggattgaaa ggattaggat agtttttagc atccaaaact aaagctggaa gggtaggatc
     2221 ttctccagcc ataccaccca ttactatagg acggagcata atttcaccct cggttacggg
     2281 ttcccaatta gttccgatct ttttatagct atagccgctg gagttcgtat ccaaacctat
     2341 cagtgaagaa ttaacagttt ccataatgcc aatgtagaaa gttgcatcag tcaattgcac
     2401 ttctgtagga agagtgatgt agttccaacc ttcgacgata gaagcaagag gatactgata
     2461 ttgagtaagt tgtacaccag gagtaccatc tactccggag ttatcataaa cccggacaat
     2521 cattggagcg gtacctactg tgtgcacata ggctttgata tacttaatgc taaactgacc
     2581 agaatgggag tatttaacag ccatctgttt tgtggatcca acgctgaatc cttgttcggc
     2641 aataccatca tcgtggttca attcataatg atcagcgggc accacgaaag tggttacagc
     2701 attggacgca ctggattcgt ttgttccata cattgcagtt acatagtaag tatgaatgcc
     2761 gccggggact tcattatcgg tataggttaa tacactgcct tctacttgag caaggtaagt
     2821 ttcatcacga tagatcttat aagcagttat ttggcgtccg cgaactggtt cattataagc
     2881 aacgccaaaa gtgccttcgg caaaagtatc gttcatagga agctcgccaa gtacatattc
     2941 tttgccatta gcatcagcta cataaacttg aatattccag ttgtaggtaa gtgttgcact
     3001 ttgttgagta agagtttgcc agacaccact caaacgaatc atatttccgt agccctcaac
     3061 ttgaggtcca ctatcacaac cagcagggaa tccacctgtt gtattacaac ggaaaccagc
     3121 ccaaattgca gttcctgagg gaatagtata aggtgtatta agaactactt cattccaggc
     3181 atcttcagtg attgtgctta caggttgatc gtaaaccata gttccggcag aaccggtcca
     3241 aattcgtaca gaataagaac acatagaagt tggctcagct gggacaaatt tgattttggt
     3301 aatattcatt cccacatagg gatagatgct attaacatca cctaaggggt cccattttgc
     3361 tgcaacatca aaatctgcag ctgaatttgt tccaacggaa ttaccagctg gttcaccaca
     3421 ataataaatc cagccttctt ctccaccgcc acctccgcta ccgggagcat tccagttaag
     3481 agtaactgaa tttccggata cggttgcttc cagattttcg ggagcagcaa taaagatatc
     3541 gttataaatc ttgaaatcgt cgatgcgaat acctgttccg ttaggtgtat cagcatcgga
     3601 tttgaagaac caacggaaga tagctgtttc accatggtaa ttactaatta aaccatccag
     3661 agtatagctt tcagtcattg aagcccaagc aggtggagca tcactataca catagttgtt
     3721 tccatttgga tcatcatatg ggttactcat atagtgccaa gtaataccat tattaacaga
     3781 catttcccat ccccaataat ctacttcagg gaaagtattg ggatcggtaa aattacccat
     3841 aatcataaaa tcagcacgaa tatcaccgct ttcaggtaat tgaatagggg ggcttaccaa
     3901 ataattcatc atattgatat tataagaacc agcatcattt tggcagatca tacaatggga
     3961 aggagatggg gcagttgcat cagttcccag atgccagata tctccacctt gaggaaccat
     4021 agaagaccaa gtcatttgac catcattcac accattatta gtatatccac caaaactgat
     4081 atcatcaacc atcattccat acattgcagg agcttcgctt gtacaatatg cagggtcgga
     4141 agcaaaggca aaacggattt ttacactttg acctacataa gagctgaggt taaaagtgca
     4201 gttagtccat tcccgtactc caccccaagc aggaattcca gttccttcac catgttcaaa
     4261 accgaaggaa tatgcagagg tcatatcgta agcgggagtt ccggttattg gagtccaggt
     4321 agttccaccg tcagtggaaa ttctcacatt acaggcatcc catccatcgt aaggtggatt
     4381 tgagcttgga gtttccacat tgtgacgcat tttgaaagtt aaagttgcat tagcagcggt
     4441 caaagtccgc gctggggtat ctaagacaag atattgatga ttgtaataac caccaatgta
     4501 agttccttga gccagggctg gatcacccat ccaccagaca tcactttggg ttccaccaaa
     4561 attgtaaata tgccaattat tgggagacac cgcaccgtcg tagtgagtcc aaccagtagc
     4621 tccagattca aaatcttcgc tataagccac aacttcatag cggctgccat ttgcaaatat
     4681 ggctgttaca agtgtaaggg ctagtataat cagccaatac tttttcattg atcctcctta
     4741 tagatcataa atgatacttt cttctaatat actgccaagg ataaaataag attactccgt
     4801 aggatttggg ttattgtata cttataccag agttttttat tagcaatatg gataagcaca
     4861 atacggataa aactcttatt taatcttggc agaacactct atcacatata taggtggtga
     4921 ccaatttcgt cgataaatta tgaaagccac cattgtatat tgatatcata attcgaatcc
     4981 caaattctgt caatcacttt ttctatatca gcccaatttt aggtatactg taatctatat
     5041 aaatcgtaat ataagccctg tttgtcaagg agttcgaaat gcgaaccttc ttcaacaatt
     5101 tcacctttat gcaacactaa aatgcgatca acattctgaa ttgtagaaag tcgatgagca
     5161 attattattg atgttctatc cttgataatt ttgtttaacg catcttgaat aagaagttcg
     5221 gtttcggtat ctatattcga agttgcttca tccaaaataa aaatggatgg attataagcc
     5281 agcactctgg caaaagcgat aagttgtctt tgccccgtag agagagttgc acctctttcc
     5341 ataaccggtt cattatatcc attaggtaat ttactaataa acttatcagc attaacatat
     5401 ttggctactt ggataatttc ttcatcggaa agctcatcat tattgagagc aatattatct
     5461 tttatatttc ccgagaagag aaaaacatct tgttgcacga taccaacatt tcttcgcagg
     5521 tcagctaaag aatagttatg tatatctttc ccatctatca gaattctacc tttttggtag
     5581 gggtacatac ccaagatcaa atttacgata gaggttttgc cactgcctgt atggccaact
     5641 agagcgattt tttcgccagg agcaactttg aaagaaatat cctttagcac ccattcgttt
     5701 tcgttatagg caagccaaac attttcgaat tcaatttcgc cttccagctt aatattttga
     5761 tgtttttctt cccgataatc atctggcgaa agagcaagta aattgaatat tctttctgca
     5821 cttgccaaag cactctgcaa gaagttaaat ttctccgaaa aatcgtttat gggctcaaag
     5881 agtttctgga tatattgagt gaaagccatc agtaaaccga tagtgattac attttgtaat
     5941 attttccctc ctccatacca aataattaat gctacagcaa tttgagatga aacatggatg
     6001 ataggacgga aaaaggcaaa cagacggatc tgttttatcg aggccagata gtatttttga
     6061 tttatgtccg caaattcttg acgcttatga aaatattgat tgaagagctg gataattttt
     6121 tgcccttcga tatgctctgc caatgtagca tttagaacag caaggtgttt tcgtacttcg
     6181 cgatagacca ctctggtctt tctacggaag ataactatta cccaaattac tagtggcaag
     6241 atactaaagc ttactaaagc caaatgccag tttaaagcca gcattaatat cacaatcgta
     6301 ataattaaaa ctacatcttg aattatagta ataactccag aagctaacat ttcatcaata
     6361 gcagcaatat cgtttgtaac tcgagtaacc aaacgtccca caggattggt atcaaagaat
     6421 tttgtaggca ttttttgcag atgagcaaaa acatcatgtc tcagatcagc cattgcagtt
     6481 tgcgaaaaat atgccgtaaa tacagtttga aaatagctgg ctacaaagcg taaagttata
     6541 atggccaaaa agataagtgc taatatcaat aattgctttg aggcatcgga acgaacctcc
     6601 aggcgttttg ctttaggtaa ttctttaagt tgttccttgg gaatagcaat agtttcagag
     6661 ctaattttaa accatccggt aagatttggt tgctcaggat ttatattttc cggcagatat
     6721 tgttttaaga tgcttatgtt tttgtcttta gcgcttacta agaataccgt atcttctttt
     6781 aaaatacctt ttgctttcag cttttccaag tccgctcggt ccattttgtt acgatctttg
     6841 gaactgataa cgagaaagtg attatcacca tatttatatt ctttaaatcc aagtttttgg
     6901 tagcggttta taaattcgtg ataatcatct tcttgggtaa aaatagccag agttttatcg
     6961 gacacgatat aatcatcaat agctgatctt tgaattaagg gaataataac ttctgcaccg
     7021 gaaattaaca tcaggaacag aaaagaaaga ataacccatt ttaggtaggg ttttatataa
     7081 tgaatgacat tgctaaaaag ccgtctatcg tatattttat tctttagctc atcttcaaaa
     7141 tgtgcccctc gtccagaatg acctccaccg ccaccaccgc caatcattat tcttcttctc
     7201 cttcgatttt tgctctaatt ctttgttttt catagaggtc tttgtacaat ccaccctttg
     7261 ccagaagctc ttgatgggtt ccctgttcgg caattattcc attattgata acaattatta
     7321 gatcagaatg ttggagcgca gaaatgcgat gggcaattat aaaagttgtt tttcctttcc
     7381 taatctctat taagcggttc aagatcctac gctcggtttt tgtatctact gccgaaagag
     7441 catcgtccaa aattaaaata ttaggattag taagcaaagc tctggcaatc gctacgcgtt
     7501 gtttttgtcc tccagaaaga gtaactcctc tttctccaac aatagtatca aatttgtgct
     7561 caaattccat tatttcatca tatacctgag cagttcttgc tgcctcaaaa acttcctcat
     7621 ctgaagcttc aggattgccc aaacgaatgt tatcggcaat tgtttcagaa aataggaaaa
     7681 tatcttgagg gacaagcaca atatctcttc gtaaaacatt taagggaatt gtaaaaagct
     7741 cataatcatc tatatagata ctattgcgtg gggggttata tatccttact aatagttcaa
     7801 ttaaagtagt tttaccacaa cctgttgggc caacaattgc cattgttttt ccagcttcta
     7861 tccccgctgt gatgtcatta aaaataaagg gtagatgatc tgcatagcgg aaagataaat
     7921 tttggaattg gattttacct tttagatgag ttatacgctc atctgcaaag gcatcatcaa
     7981 tttcaggatt aacttcaaat atatcattca agcgtttcag ggaagcagtt ccgcgttggt
     8041 acatatctac aatatggcca atagcaatca taggccaaac tagcattcct aaatattgga
     8101 agaaagcaat aaaaccacca acgcttattt cgccacgaat ggctgcctta cctccaaaat
     8161 agagagtgat aatcatactt atactgataa taaaacccat aaaaggatga aatacaccag
     8221 ctattttcgc cagcgctaaa ttctgatcta caaaatcgtg agatacctta tcaattttct
     8281 ttaattcgtt tttttcctga cagaatgcct tcactattct gatgccagaa atggtttcct
     8341 gaacttttcc acttaatgta gcaaaacttt cctgcacagc tttaaaccga gtatgcacct
     8401 ttttcccaaa atacaaaaca gttaaggata taataggcat aggaattatt gccagtaatg
     8461 ttaatttagc atttatcgaa accataaatg aaaaagaggc aatggtcatt agcacaatat
     8521 ccattgcaga aatcaatccc attccaaaaa gcatccgtac cgcatttaaa tcatttgtag
     8581 cataagccat taaatcaccg gttttgcttt tattaaaaaa attctgagat aaggttaaca
     8641 aatgatcgta gtaatcctga cgcaagctct tttctatgag atgcgaattg ccgattatca
     8701 gaatccgcca aaaatatctt agtaccatca ctgctatggc acaaccgaat ataattagcc
     8761 caatccaagc aagacctctg tagtcaattg tccgttcctg gatgtgatct atggcatatt
     8821 gcatcacttt agggacagca agctgcacaa gatcaaccaa aattaacatt accaatccac
     8881 ccaaaatccg ggtgtagctc ttcttcaaat agggaagaac agcatcaaat gttcgcattt
     8941 tgctcccata atcaattatt tggggatgaa agctaacctg aagaacaggt gctatagact
     9001 attaaaaccc aaagtcaacc tttagaaatg gataattttg tcaaatgttt tcagttgata
     9061 ttgcatattc ctatatggta aaaaaccaat gtactatcat aaaatagggg atttttatag
     9121 tatcgagatt ttaacaaaca gattgaatct tgcttagata gttgtggtag aaccaccgtg
     9181 atgctatgga gggaaatgcc caagaaatac caccgtattt ctaccacata taattaagca
     9241 agaaatatgt atactgagtc cttctgaaaa aagtggacca aaattacgaa gaaagaaatg
     9301 aacaaaaagt ttacctaaaa ggctgcatat tcaggatagg atgccatcga atatataagt
     9361 tgctcatatc tgggcattca gcgtgaatta tttttgcgga agaacggcgt tctccgacta
     9421 cccagttgtc atcttcttgg tatactcttt ttaaatctga gagtattatt ttcggtgact
     9481 tcgatggcct cggtggctaa atcctctcaa tccttaaatc ctaaaaatcc ttgtttctat
     9541 aattaagcca gcgtgaatta tttttgcgga ggaacggtgt cctccggcta cccatttgtc
     9601 atcttttttg tatactcttt tcaagccagt gtgaattaat ttagataatt tggtttcatc
     9661 aagcgagaag aattttgatt gacgataaat gccttgtaaa aaactatgtg tcttacaaaa
     9721 aattatggtg agacaatgaa caagcttttt atgcctgagc tggtaaaaat tatagaaaaa
     9781 ttcgagaata aaaatgcctg tttacattat atggcagacc tgctatcgga aagcggttgt
     9841 ttaagttttc cagatcgttt tttggctgca gtaaaaggta gagaagaaat aatgagtact
     9901 ggtattggaa gaggaatagc aattccgcat gcaagagatc taactgttag tagtttaaaa
     9961 attgcagtat gtatgataaa gcaaggttta gattttacaa gtgtggataa tttacctgta
    10021 aatttggtgt ttatgattgc tgtgcctcaa agttccaatc aggattatat gaagatatta
    10081 cgctcgcttt caggcttttt gaggcgggat gaaaaccgtg aatcgttatt acaagcaacc
    10141 gatgaaagag atctatatga aaaagttcag gttcttgaag aagagcttcg ccctcagctt
    10201 ggcgtttagc tttcttataa tattaccgtt attttcacag catcaggatg cgggaacgac
    10261 cggattctct actttgaaga tttattattc tgctcgagct aatgctatgg ggcaggcaat
    10321 gctggggaga gcgatgaatt ttgaagggat gcaattcaat cccgctaccc taataaaagc
    10381 taaaaacaag gctgtctata ctacttttgc agatcatttt gtaggttctg gaggcggatc
    10441 tataaattat atggttccca agaatatcta tacttcctat ggtttttatc tcaattactg
    10501 gaattctggt tctatcgaca gaacggatat cggagcaaat ggcgagttta ttgaattaaa
    10561 cgatactttt ggagcacagg atattaccgc tgggttttct gtggccaaat ttgtcagtcc
    10621 tgctttagat gcaggtggaa gcgtaaaact tgttttagat caaattgatg gtaaatctgc
    10681 tactgctgcc ttaattgatc ttggaatttt acatcacact cccaatgaaa aaataaaagt
    10741 ggggcttact atccgcaatt taggctttca attgagccat tatactaata aagattattc
    10801 tgaaggaata ccgattactt ttggtgctgg tattggaatt gatgcagcaa aaaatactct
    10861 gctcaatata gacctaaata aggctaatgg agaaaacttc atcgggaaat ttggcataga
    10921 acaaagtgtt catccttctc tggtgctgag agctggtttt aaaacaaatg gtggcgatta
    10981 ttctataggc ggcgatttgg gttggagctc tggaatcagt ttaggcttgg gatggacttg
    11041 gaaaaaatat gcgattgatt atgcaattgc ttcttatggc gatttaggat ttacaaatca
    11101 ggttagtata cgctataatt ttgactaact atagacgact atatattaag gtataggaaa
    11161 acttttgaaa cagaaccaat ttttacttga attgggagtt gaagaacttc ctgaaaaaca
    11221 gattactatt gcttgtaata gtgtaaagct atcttttgcc aattttttaa aggagaataa
    11281 acttaattgc tcgggttata aagtgagtgg aacaccgaga cgaattttct tgcaggcaat
    11341 agacctggat agccatcaaa aagatgaaga aattgttaaa acaggtcccg cttttcgcct
    11401 cgcatatgat gaggcaggaa atttaactaa tgccggaaaa ggttttttga aaagaaatca
    11461 ggtggaagaa agcgcaatat ttattcagga aacagataaa ggtaagtttt tagcggtaaa
    11521 atatatcaaa accgggatat ccacaggaga acttttacag aagtggatcc cggatatgat
    11581 tttacagata cctttcgata aaaaaatgct gtggaataaa cctggttttg ctttttcgcg
    11641 tcctttacgc tggcttctaa ttctgcttaa taataatcct cttactatag atttttttgg
    11701 tataccttgt gggaatacaa cttatggtaa tcgctattta ggtttggata gatgcattac
    11761 aataaataaa gctgaagaat atatccccaa gctgctggaa cataaagttg tagctgaaat
    11821 tgatgaacgc cgcaaagaaa ttgtagacca aatggctaat atctttccca aaggtgatta
    11881 tcgggttata aaagacctcc ctttagtgga aacggtagta aacctggtag aatttcctac
    11941 agcagtttgc ggagaatttg aacacaagta tctatcacta cccgataaaa ttattatcag
    12001 tacaataagt caggcacaaa aatattttgc tgtacacgat acaattggca agctgagtaa
    12061 caagtttgtc tttatttcca atggtgatcc agcagcctgg gatcttatta aacagggaaa
    12121 tgagaaagtt gtaaatgctc gtttagctga tgcattgtgg tattggaacg aagatacaaa
    12181 acatccactg gattattgga ccaaacactt ggatatggtt gtctttcaag caaaattggg
    12241 aacaatggct gataaaacaa agagaataat ttgcctaact gagcttatag ctaaagaact
    12301 taatctacct tcagaacaga gagaaaaagt gcttcgctgt gcagaactgt gcaaagcgga
    12361 tttagttact aatatgctag gcgaaaaaga atttaccaaa ctccagggat atattggtaa
    12421 acagtatgct attgtttccg gtgaagatga agaagtagca gaagggatat atgaacacta
    12481 tatgccccgt ggttctgctg ataagctacc ttctactttg tgtggagctt tggttgctat
    12541 tgctgataaa atggatactg tggctggaat tatagggatt ggtatgcttc ccactggaag
    12601 tggagatcct tttgctcttc gacgtgcagc caatggtata gttcaaatta tcagccaccg
    12661 aaaatgggat tttaatatct ttgctttagc tgatgaagca ttggaactaa tcagtaagca
    12721 aacggaactg gataaaaatg cttatagcaa tctacatagc tttattgaac aaaggataac
    12781 cggtttgctg aaaacaaaag gcattgctta cgatgtgatt gatagcgtta tgcatatcga
    12841 taaaagccat atatatgatc tggaaaacag agcgaaggct ttgaatgatt taaaaagcaa
    12901 accaaatttt attcatttgg tgatcggttt taaaagggta gccaatatta ttgcagagac
    12961 cactgaattt gtcccctttg ataaaaataa actggttgaa ccggaagaga ttaatctata
    13021 taaatcgttg caaaaattgc ataatgatat agattctgct ttacaaaaaa aggattatcc
    13081 tctggctttg caatatctga tctcgtttgg caaagtgata gatgcctttt ttgatgctgt
    13141 tttagtaaat tgtgacgacg aagttttaca taataataga cactccctgc taagggaagt
    13201 gaaaaatgag tttttaagag ttgccgatct atcgttgata gtattggaaa ccgaataaca
    13261 ggaagaagaa atgttcataa ttgacgaagt gaaaaaaaaa gctaaaaaaa ctaagggcag
    13321 cgtagtttta cccgaatcat ctgatcctcg ggttttaatt gctgccgctc aaatcgtgaa
    13381 ggaggatttg gcaaaaataa tactattagg caaggaggaa aagataactg aggatgctaa
    13441 agcattgaat ctggatttga atggtgtaac gataattaat cctgagacct cagaatacct
    13501 atctgctttt gctgatgcct tttatctgag gcgcaaacat aaaggagtaa atcctgtcca
    13561 agcattggag acggttaaag atccccttta ttttggcgct tctttagtaa aacagggaaa
    13621 ggttagtgga atggttgctg gagctgttaa tacgaccgct gatgttttaa gggcggctct
    13681 acaggtgatt ggtgtgatgc ctggattgaa aactgtttcc agtactttta taatgccggt
    13741 tcctgatttc agaggtgaac ccaaggtctt tttatttgcc gattgtgctg ttgttccaaa
    13801 tcctactgct gaacaacttg ctgatatagc tatatgtact gctctaacgc gaaaagcaat
    13861 tattggagat gaaccaaaag ttgccctgct ttctttttct accttaggca gcgcaaacga
    13921 tgaattggtg gataaagtta tatctgcgaa agcaatttta gatgaaagaa aggtagattt
    13981 tgagtatgat ggtgaattac agcttgatgc agccctcatt ccagaagtgg caattagaaa
    14041 agctcctcaa agtcctattg ctggaaaagc taataccctt attttcccgg acttgcaagc
    14101 tggaaacatt ggttataaac tggttcaata tataggaaaa gtaaaagcca taggaccaat
    14161 cattcaaggt ctggctgctc ctgcaagtga tttgtctcgc gggtgctctt acgaagatat
    14221 tgtgaataca gtggcttatg ttcttctttt agctgctcaa caaaaatata aataaaataa
    14281 aaaaatactg gaggcaaaga tggccacaaa gctatccaac cgctgccgtt tgattaagcc
    14341 ctcgcctaca cttagtttat ctgctaaggc gaaggaaatg aaggatgctg gaattgatgt
    14401 aatcagtttc agtgtcggag agccggattt tcctactccg gattatatta aaaatgctgc
    14461 tcataaagct attgacgcta attatactcg ttataccaat aatgctggta tcctggaatt
    14521 gagacaggca atttgtgata aacttctacg agataatgat cttaaatatt cccccaaaga
    14581 aatcctggtt tcgccagggg ctaaagcatc aattgttaat gctttaactg cagtatgtga
    14641 tgtaaaggat caagtgttaa tgggagcacc ttattgggta agttatccct atcaagttgc
    14701 cctagccaat gcagaaccaa tatatattcc taccaaagaa gaagatggct ataaaattcg
    14761 agctgcagaa ctggaaattg ctatacagga aaatccttgc actaaagccc tgattctaaa
    14821 ttctcccgga aatcctactg gtgctgtgta tacaaaagaa gaacttgctg ctcttgctga
    14881 aatatgtatc aaatacgata ttctggttat ttccgatgaa atctatgaac gcctggttta
    14941 tgacgatgta aaacatatat ccatagccag tatcagtgaa gaaatgaaac aacggacaat
    15001 tgttataaac ggagtttcta aagcttatgc gatgacaggt tggcggctgg gttatgctgc
    15061 aggacctgca aatattattg ctacggcggg gaaagttcag gaacatagca cttcctgtgt
    15121 aaattctatt actcagtatg cttgtgtaac agctttaaat gaagaagatg attccatcga
    15181 aaaaatgcga caagaatttt ctgtccgcag agattttctc tataacgaac tgaaaaagat
    15241 tcctcatatt tcctgtttta aaccccaggg tgctttttat attatgccag gcataaaatg
    15301 gtatttggaa aacaacaatc agaatataca aacttctgat gatttttgtg ctaaattact
    15361 tgacaaatat agtgttgccc tagtacctgg tagttctttt gggatggaag gaaccgtacg
    15421 gttctcctat gcaaacagta tggaaaacat taaggaagga attagtcgct ttgcttcctt
    15481 tttacaggag ctaaatcctg ggaggtaaaa gatggatgat tttaacgata aaatgaatga
    15541 tcgctggaaa gaaaagcgca agaaagcata taattggcct agactgatta ttatgatact
    15601 tgctctgata gcaattcttt acattatgaa tcatctggga aatactaaaa atgtggtgca
    15661 aactccttca gcaactgtaa cagacacact tcaggcagat tctgtgcaca aggagataac
    15721 accttgagtt tagttatcgt tggttcaatt ggtttagata gcatatctac acctgcagga
    15781 gaagtttgtg aagctttggg tggttctgct atatatggtt ctttggcggc ttcccatttt
    15841 accaatactc atattgttgg tgtagtagga aaagatatac ctgttgaata tctaaacctg
    15901 ctgcaaaaaa gtggcatcaa tttagatggc ttagaggtta aagatggtaa aacttttcgc
    15961 tggaaaggga aatattctac ctggaataga gtggaaaccc tttctaccga tttaaatgta
    16021 tttgccgatt tttctcctca gctgcctgaa agctgtaaat gctgtcgtag tttactgctt
    16081 gccaatatcc atccagcatt gcaattacaa gtgctaaatc aggtatcggg atattcgcat
    16141 ctggcttgtg acactatgaa ttattggata gagctgtgtc ctgaagaagt agaaaaagtg
    16201 ctgaaacggg tagatatagt ttttatgaac gaggatgaaa ttcgggatta tacgaaaatt
    16261 gcggatatct actctgctgc gtatgaaatt ctatcattag gtcctaaatg ggtaattgtt
    16321 aaacgcggtg aatgtggttc agtagctgtt tctaaggatg atatgtattt tgctccggct
    16381 tatcctgtaa aaaaagttaa agacccaact ggagcaggtg atagttttgc tggtgctttt
    16441 atgggctatc tggctgatca cgattttgtg aatcatgctt taattaaaga agcaatcctt
    16501 tatggaacag ttcttgctgc aatgaatgta tcagatttta gcgttcaggg tttagtgggg
    16561 aaatcgatga gcgaaattga taactacaag gatagattaa ttaaatggac atcctgaaag
    16621 acagcttaga ttatcgcagt tccggggtag atattgcggc tggcgaaaaa gccgtaacag
    16681 ctataaagca taaagtaaaa gctacctata atagcaatgt gttaagtgaa ttaggcagct
    16741 ttggtggctt gtatcaaatc gataaatcca gctggcaaaa tcccattctc gtgtctagca
    16801 ctgatggagt tgggacaaaa ctacttgtag ctattctggc taatgagtat aaaacaatcg
    16861 gtcaggacct ggtaaatcac tgtgtgaatg atatcttggt acaaggtgcc atcccccagt
    16921 tctttttgga ttatataggt ttgggtaaat tgattcctgc aagagtggaa gatataatag
    16981 aaggtatggt agtagcttgt aaacaaaatt cttgtgctct aatcggggga gaaatggcag
    17041 aaatgcctgg aatatataaa gaaaaggatt ttgacctggt aggaacaata gtaggaattg
    17101 tggaaaggga acaattgctt cccagagcaa aaatgaaacc tggagataaa ttaattgctc
    17161 ttccaagcag tggattacat actaatggtt attctctggt gcggaaaatt gtatttgaaa
    17221 agcttaatct atcgctagat acatatatac cagattgcga aacaactttg ggcgagcttc
    17281 ttctaagggt gcatcgcagt tatcttcctc tgttgaaaga ttatcttacc gatccttctt
    17341 tattaggact tgctcatatt acgggtggtg gaattgctgg aaatctgaaa cgcatccttc
    17401 ctgataatgt gtctgcaata attaacctga aagaaacaga tattcctcca ttttttaaat
    17461 ggttacagga agcaggtaag gtagatttct ctataatgaa ggaaaccttt aatttgggaa
    17521 ttggaatgct ttgtgtagta gatcataaaa tgcaagataa gtatttatct atacctgaag
    17581 caagatgtgt aggagagctt gtcccttcag actatattca ggaaaaaatt gaatttgatc
    17641 tgtgtggtgc taaataaatg tcttatgtaa gtttctcctt gttcaaacct catttttggt
    17701 taaaatatat aaaaaacctc ttgacgattt tgtatgtatt aaagatgaag tttttaaata
    17761 ggttttttaa aaaaagatcg aaattagttc cttctactaa aacaaaagaa ggtgaaataa
    17821 agaataccca taaagaaagg gaggttttcg atgcgcaaat atgcattgat cctgctaata
    17881 atcatgctta gtggagtagt ttttctggac gctgccacta ctgggaaact ttcagttcga
    17941 gtacgtgata actcagggcg tccactcgaa tttgcgaatg ttgttgtctc acaaggaaat
    18001 caacgcatta ctggtggcca gaccaacgaa aaaggattag caattatcat aaatattcct
    18061 cctgggatat atactgttaa gatttcattg attggctacg atacggtaat ttataatgat
    18121 gtccggatta aggttgatga aacaacatct cttactccca caatgactaa aagcgggata
    18181 gaaatgaagg cagttactgt tactgcaaaa gtggataatg tggataaaaa ccagattgga
    18241 agtgctagaa ctatagagat ggatattatg accaatgctg cagtatccga tgtttctgat
    18301 attgtttctc ttcaagcagg tgttgttaat attggaggcg aattacatat ccgcggcagc
    18361 cgtgataatg agataaatta cactgtagac ggaatgagcg ttaccgatcc tgtggacgga
    18421 ggagcaactt tacaagtgga tactgatgcc attaaggata tgaaggttat gactggtggc
    18481 tttcctgcag aatatggtaa tgcccaagca gccgtgatta atattgttac taaagatggt
    18541 gatccttttt tctctggtaa agtagaatat aatactgatc atttaatttc ggaaggtcgg
    18601 aattctgatg ttttaaaatt tgctattgga ggtcctgtat tacctttcgc ttctcagaaa
    18661 atgaaagaaa aattcacttt ttatcttaat ggagcaggtg aatggttgga tggccgtttg
    18721 aaagattatt atatcagcga tcccaataag gattattcta ttaacggaag atcattaatc
    18781 gatgcagatt atgaggagta taatccttat tcagatagag acaacatact aggtatcgat
    18841 attgggaaca gaaactttaa ttcctataat ataaacttga aatctaagta tgctttcaaa
    18901 cctggacagg ttgccacttt tgctattcga ggcgatagaa acttaaacta tctatataac
    18961 aatacctggc gttatgcttt acaacattat gctgaagaag aaacagatca acggcaatat
    19021 attggcacct atgatcgggt tattaatgac agaatgaatc tgaaagttaa agccagttat
    19081 tatcaaaaag ataattatga aggtcctagg ggaattgaca ggaataacta tatgtatatg
    19141 acaattgatc cgaataacat acctgctaat tacattgata tggtttcctt aggaaattat
    19201 ggatatacta ctattgatgc caataacgat aatgtacacg atgtgggatt tcttcctgct
    19261 gaatattggg tctaccgttt ggaaagtgtg gaagaaccca gaaacattcc tggctataac
    19321 cctcctggta caatttatag tagatatgtt gatgacacaa ccaaaattat cagtactcgt
    19381 gcggattttg aatggcaggt caatgaaacc catttagcta aaactgggtt ggaggttatc
    19441 aagcataaca ttatgaaaaa tcagctggaa gattatctta aaattgatga aaaacgcaga
    19501 accaattacc tgaatagtat tttcaatgtt agaaattttg atcttgataa ttatgtggat
    19561 accaatggtg atctgttatg tgattatgtt cctgaagggt tatatgctct ggctgcaatt
    19621 caagaggttc ctacttcttt agcggattta gtacctatat actatcctca agattactat
    19681 gctgcagcaa aggcagctgc tggcaaacgc gatggttata aagctgaacc ttggcaaatg
    19741 gcctattatt tgcaggacaa aatggaatgg gaaggtatga ttgtgaatgc tggattacgc
    19801 tttgatttct ggtatttagg aaaagactat tctgtagcca gagatgatgg tagttacgat
    19861 tcccgcgcct ttgatgaaag tgatcggcta caagttatga tctctcctcg cttgggtgtt
    19921 tcccatccga ttaccgataa ggatgtgctg cgctttgcat acaattacca aaatcaatta
    19981 cctcaaatgc aatatatctt cactagtaaa actccggaag atgctaacga ttcggataca
    20041 attatccaag taggaaatcc tagtttggaa cctcagatta ctattactta cgaagttggg
    20101 ctttctcatc aacttagtga agattatgtt ttagacctaa ctgcgtatta taaaaattta
    20161 tataactatg tgaatacaag aaaagagcgt agcgaaattg agcaaacagt ttattggtac
    20221 agatattatt ctaatgatta tggctctgct cgtggtatag atatgcagat agagaaactc
    20281 ctctctaact ttaacacttg gagccttgct tattcattag cttgggctca gggtaatagc
    20341 tcctatacag ttatccaaga tgagacaact aatttgagag agtttccttt ggattgggat
    20401 atacggcata atataagtgc taatttcact ttccatattg caagaggcga ggaatacttc
    20461 attccgttta ctgattatat tttacctctt gatgattttt ctgcgaattt taattggact
    20521 tttgcttctg gtgctccata cactcctcaa agtgcagaat ctaatagttc attagatact
    20581 aacagcaaaa gaaaagataa tactcagcaa gcgaatttga gattaactaa gggttttgtt
    20641 ttgcctaata atatgagtat cagaatttat gcagaaatag aaaatctgtt taaaaacatg
    20701 aatgttctaa atgtttatcc taaaacaggc aattattatt ggggcgggga caacctggaa
    20761 gaagctaata ttcctggatt tgtttatgat gaagtacaat atgtttatag cctcgcatac
    20821 aaaaatccca cttattataa caatttccga gggcttactg tgggcgtctc gtttaacttc
    20881 taaattattc caaggaggaa ttaataaaaa tgagaaaatt catcggaaca atactgttga
    20941 taatgatgat gacctttgta ttcgcgagtt tcgcttatgc acaggaaaat actgctgcag
    21001 ctgatcaagg tggtggcggg ttacaaaata ttgcagagct cgtatttggc agtggtctgg
    21061 ttaaatggtt caaggatggt ggatgggcta tgtggccaat actcttcttg gctatctatg
    21121 gcttagctta tattatctgg aagtttattg ccttacttac tggtaaagtt aatttaaacg
    21181 cttttctcaa caagatcgtt cctttaataa aagataaaaa gtatgaggaa gcgaaagaaa
    21241 ttgccagtaa aacaagggga cctgtagcat caattattta tgcaggatta gaaaaaatag
    21301 ataaaggaat tgatgctgtg gatgaagcta tcgaaaatgc ttctatgatt gaaatgagct
    21361 atctggaaaa gggattcatt gagctatcta attctataac acttgctcct atgttaggat
    21421 tccttggtac cgttgctgga atgatcactg cattcgattc tattgctgct gctcgggctg
    21481 tcgatgctac aattgtggcc acaggtatta aaattgcact tattactact gaagcaggat
    21541 tgattgtagc tattcctgtt cagttcttta ataatgtgtt tatgactatg gtagatggaa
    21601 tagttataga tatgcaaaga gcatccgaaa aggttacaga aatactagcc gaaagcatag
    21661 gtactaaaaa atgaaaatta ctcggaagag gaaatacatg gcagctatcc caacagcttc
    21721 cactggtgat attgcctttt tgttgatcat cttctttatg tccactacca agtttgatgt
    21781 gaaagaaggt attaagcttg ttcttccaca ggcggctgaa gtggaaagtg gaaaaactca
    21841 aactcttacg cttactgaga aggaaatgac tcgtattcaa atcctacctg atgggcaaat
    21901 agcgttaaac agggaagcac cgcgatatat tgaaagtagc gaactggatg ttatcattca
    21961 gcagaaagta caaattaatc cagatatgat ttttaaggtc attactgacc gtgaaacaaa
    22021 atacagcgaa atgattagag tggttgaccg cttgaaagct gctaagattg aaaaaatctc
    22081 cctatctaca agttaggaga aagaaatggc taaatttagt acaaaaaaaa gacgtgatgt
    22141 tgccattcct caggcatcta cctctgatat tgccttcctc ttgttgctgt tctttatggt
    22201 gaccacagtt tttgtgcagg aaaaaaagtt ctgggtagaa cgcgatcgtt ggcctcacgc
    22261 agaaagtatt gaacgcattc cgcgcaatca tacaagtaca atctatgtta tgcgagatga
    22321 aactatttta ctggatgata ttcctacccg gatagagagc tctgaggatg tattcatttc
    22381 acaaaccctt atccgaaaaa aagcggaaga tcccgagctg atagtttgtt ttcgaacaga
    22441 tagagacacg aaatatggag taatgtctga tataatgcgg caacttcaag atgctaacac
    22501 attggtagtt gcctttgaaa ctcaaccatt gctaaaataa aaggtggata agatgacaac
    22561 acacaatcaa gattggaaag acattgctaa tgcacaattt agcaaagcac tggctttatc
    22621 tcttaccttg cttatctttg gcataatggt aactccgaaa gttgaagttc gtaaacaggt
    22681 ttttcaaacc caacaaatgg aattggtcga tattccgatg gaagaacgag aacggataga
    22741 agcaccgcaa acagaagttg atgttaatgt aactttccaa atcagcgatg tattaggaac
    22801 tgaagaagtg gatatggctg cttttcagga agcactttcg cagattggag atatctacaa
    22861 aacaacaact acaagtgttc aacaacaaga agatgagaga gtggtaaatt tcgttcccta
    22921 tgatgatgct ccagtaatta tcggtaaaat tgaacctgtt tatcctgaat ttgctaaacg
    22981 aaacaaattg cagggaaccg tagtattgga agtggaagtt ttaaaagatg gcagcattcg
    23041 tgatataaga gttcgccgcg gggtaggagg cggtttggat gaagctgcta ttgaagctgt
    23101 gcgtaaagta agatttcaac ccgggaaaag tagtggccaa gcagttgact gtttggttat
    23161 aattccggta gaatttaaaa ttcaataaat tgggagtgct taatgaagtt gtataaaatt
    23221 ggaatttgcc tttgcctact ccttatattt tctataagct cagtatatgg agcgaaggca
    23281 gttccctcaa ctggaacaaa aaaactggac ctaacgaaat atcataatgt tggaaatatc
    23341 tggttaaggg taagtaacta tggatttttc ggttcaggtg atgatattgt tccccgatat
    23401 ccttccttag agtatccagg tggtagcggt atggattacc tttatcaggg agctttatgg
    23461 tttggagcca aaaaacagcg tcgtaacaat ctaaaccaaa aactgtattg gttaacatat
    23521 ccaccctcag ctagcaacga tactcttatt accgaattag accctcgctg gaattcgaat
    23581 aaagtagcag cattggatac attagtaagt gttggttttg atggtgatga tgatttatac
    23641 gaattcttac cagccttcaa tcccttggtt gttgccaatc ccgagcaatc cggtaactat
    23701 gctctttata ataaccaaga tggcataagc tatgcttcga ctaggcaaca aaaaagagga
    23761 aaagacgatg atggtgatgg taggatagat gaagatggag ctggttttac attccccttt
    23821 agaatggcat cggaattacc aattcaattt gctaatgatt tcggcggtag atttatgtat
    23881 gaagttccta tatccgcctt ctctattctt gatgatccta ataatgcgga aatatggttc
    23941 ccgcttggtt ttatggaact atcttaccgg agtaacgatc ctacttataa ttatgctttt
    24001 tcagctcctt ttgatgatga tggtgatgga agagtagatg aagatggagc tcctgtttct
    24061 gaacaggatt atatctcctt ctattatgat tattgcccct ttggtacaga tggagaacgt
    24121 gattacggag ctagcaaggg cggtaacact cacataccta taaaggttag agtgcgccaa
    24181 atgagctatc aatggagcta tgattatatt aaaaatttag tttatgtgga atttgatatt
    24241 accaatatga atgatctgga tgaattgaaa gattgcgcaa tgggtatata tatggattgt
    24301 gatgtcggtc ctcagacata tggagtggaa aaagcatctg atgataaatc cggttatgta
    24361 aaaggtccag gttatgaatt tgcttatact tatgatgccg atggtgacaa tggcctttgc
    24421 actggattag taggagctcg tgtctgtact cctgatcctg aaaaattgag tttcgattgc
    24481 tggtattgga aagtaggaca gggacctgat gacagccgtc cccgtaattt aagtccttca
    24541 aacaaaacag caaatgaaaa atactggctg cttacagggc gtaatcctaa tactacatat
    24601 tacactccgt tgcgtccaga agctgacgac cagaccgaat gggaacaacc ctctccaaat
    24661 gatacccgtt tccttttctc attctatggt gcccaacctg gaactgagga atataatgaa
    24721 caggatgaaa atgggaatta tcacaagcgt tggaatctgg cacctaataa aacaatgaaa
    24781 attgtggttg ctgtatttcc tggtgataat aaagaagatc tgaagaggac agctcgttgg
    24841 gcaaaggaaa tatacggtca agctcaagat ctagtcactg tggttctacc ggatactttc
    24901 ccgcactata atcctccgga accacccgaa attcctacta tgtatgcaga gatggttaac
    24961 gatggtaatc aaataaacat ttattgggat aatcgcagtg aattctccta cgatgttaaa
    25021 actgtcagta ctgctgttat gggctggcag gatcctaata gtgcgaatat tattagcgga
    25081 ttagatagtg atcctaggcc tcatatagct aataattggg ctgacattcc tgaacaattt
    25141 aggccagatg aatccttacc tcctgataaa tgttggaata tgaatgcctt ggtaaatcct
    25201 tttacggcat accgtttacg ccatgatttt caaggttaca ctgtttgggg aagaagtggt
    25261 agtggtagcc aggaagattg gtctatgatt gatcgttggg ataaagtaga tactgctcag
    25321 gacttggctg actattcagc taattatgga tatccagatg tatttgttga ttttggtgga
    25381 tatatgggta tcaatacagg tttacccaat aaaaatgcct ggaatgctcc tttaagtgaa
    25441 tataataaat tctattgttt cgacgaaaat tattctcttg tgcccaatgg agatactttc
    25501 tatggctggc ctatctatga accaaatcca attataaatg gtactcctat ggatgaatta
    25561 accgattggt ctacaatcca agaagctgca gatgaaattg ttggtccaga tatggaaacc
    25621 acgaaattga tgcaagcacg gctcttcaag cataatgaaa ttccccaaca gatatttgat
    25681 gctttatatg atccgaaact tattccattg cgtggttttg cttttcctgg aggaacaggt
    25741 aatgaaggac ctgatcagga tgaattactg gaattgaaaa aagaaagatt agccagacgc
    25801 tactataaat cggaaatact atatccccga aagggaatcg aatattatat tgcggtcact
    25861 gcttatgatc gtggaattcc atctaacgat ttaaacttct tagaaacagg ccgtgatgcg
    25921 gatgccaata tgagagtatt tttccctgga actttagcaa aagagaatat ggataaaata
    25981 atggttgttc ccaatcccta cattggacac agtagttttg acggccgcag agagaatgat
    26041 gacaagggag ataaaagccg tcgtatatgg tttgttaatt tgccaaagcg ttgtgacatt
    26101 agaatctata ccttagcagg cgatcttgta caaaccttaa aacatgatgg tgccccgata
    26161 accgatatta taaccatctc gaaagcaagt acagcaggtg ttgctgctga tgggatggaa
    26221 tgctgggatc tattatccaa acatcaacag attattgctc ccggtgtata tctatattct
    26281 gtagaaaaca aagccaacgg tgatgtgaaa gttggcaaat tcgtcataat taagtgaggg
    26341 aggaactgtg aagaaaagaa tgattattat cataacgata gctctcgtgt gtgtaccact
    26401 gcttattgtt gccaaaccct ttggtaaaac aggcacagtg gctttacagt ttctcaagct
    26461 gggagttgat gctcgtgctg taggtatggg tgaagcatat actgcagtta cagatgatat
    26521 ttcttctgtt tattggaatc cagctggttt agctcctgct tttgaaaatc aggtatttgt
    26581 ttcgcataca aactggcctg cagatattat gcatgaattt ggagctgcga cctatacaaa
    26641 tggcgtttct actatagcgt tatatggcag tgtattacat atggatgata tggatgtaac
    26701 ggaagaagaa acattaggtc ctaccggcga aaaatttact tgtagtgata ttgctttagg
    26761 agtagctttt gctcagcaat ttaccaataa attctcagca ggtgtgggcg ttaaatactt
    26821 gcgagaaaac ttatatgagt ttgatataaa tagttatgca gttgatcttg gttcaatgta
    26881 taatactggt tggcgtaata ttaagattgg aatggcaatg cgtaattttg gccctgatat
    26941 ccgttttaga gtagatgacg atgatgatgg cagctatgat gaagatccat tcgatttatt
    27001 cgataatgat ggtgatggac ttattgatga agataggatt gaattggaaa gtaaaatacc
    27061 tttaggcttt tctctaggta ttagtggaga tattatgcgc caaggaaaca attatatgat
    27121 tgcgtccttt caaatggata atgtaattga ccgcacagaa acttggaata tgggagcaga
    27181 agtgaagctt agcaatttat tcctaagagg cggttatcag tttaactatg atactaatgg
    27241 ctttagtgct ggagtagggt ggcaggttcc tacctccctt ggaattttca atatcgatta
    27301 tgcctatacc tctatgggcg aattagcaga gagctttatt aaaagcgctc atcgtgtatc
    27361 tattaaaatg aaatattaaa atgaatctac taatgattca ggaggaaaat atgaaaaaag
    27421 ctcttctaat caccttatgt ttgactttcc ttgttggtat cgcttttgca caacaaatgg
    27481 acccggctac caacaagaag gcactcgaag gtcctgagat gaaaccttgt atattgcaga
    27541 acagccgtac tgttcctcaa tatactttta ctcgtacccc ttacgcattg ttgacctcat
    27601 attatgatta tatgatagga agttacaatg gtattccttt gcgcgtgata ccatcagata
    27661 tctctgatgg tggatatttt atgacatatc atgcacgtag ggcagctaat gcaactaggc
    27721 gtatatttta tgcttactta ggagcagatg gcagtttaac tactaatagt gaaatttcta
    27781 atgtcacagt aaatgagggt tatccaactc ttgcagttga tccaatttct gggaaacctc
    27841 tatatgcttg gcatgcaaat gcagattctg aagccgatta tttagaaacg caatttacag
    27901 ctgattcttt tattcaaggt actgacggtg tgttcttacc gataacaact ctaattgata
    27961 atcctgtaac atgccttaca acaaacgaca atgaatatat ctggcctaca gctcaaattg
    28021 ggccctcgcc tatagctggg aaaagcagag tttatatagt tgcaagaaat gcaactactc
    28081 acacttatgg accttcagag aatccgcgta ttgcctatgc tgattttgat ggagaccaaa
    28141 ttgaactagg agagccagcg cttgattggc actttacctc tattcccgaa atggaccaat
    28201 ggaatcatga ttctgaatgg cgtcgtccat tccatgcgtt aactacagac gatgcgggaa
    28261 atgtttatta cgctggtttt cattttgcta cagaatctga tggatcaact gatattcctg
    28321 agccggattt agatgtgttt aagtgtaaca actttggaga aggaacttgg acgcgagtta
    28381 gtgaatggag caagatgcct tcttggaatc ctaataccag ccctacagac actactggcg
    28441 tttttgaaat ttcaggtggt aatagtcctt atggtgatga tggattatat tggagtgtaa
    28501 atggcagcgc ttcgagccat ctgaatgcaa ctattgatga tttgggaaga attcattacc
    28561 cagctatctg gtcattaaat aatattgatg gatattacta tcctgcatat caatatccca
    28621 aagagattat ttttgatcct tctgaagcgg aaggttccca atttctgatt aatgaaatct
    28681 ggcctaaaaa ggatcctcat aataatgtag atcagtgttt tcaaccttgg gacactgaag
    28741 aaccatgggg agaagaagaa tttgctcaag atcccgatac aggacaatgg tatgttagtt
    28801 cataccttag ctgggatttc ccttattggg atcaaagtgc acatggtgat gctatgtttt
    28861 tccattgcaa caatatgaaa gtaaccgaag gtaatgacca cggaatgtta gctacagttt
    28921 ggcaaaattg ctatcgtgcg aaaagattca atgcagatgg tgatactgat tatgctgagt
    28981 gggcaaacac tcctgaaatt tggatttcag tttcctccaa taatggagat aattggagtg
    29041 agccaatttc attgaacaat attgaaactc ctgaatttgc tggacttaaa ccgatgtggg
    29101 tttatcctgc tgataaaatt atctatgttg gagaacagga tggcttaaaa gtaggtaaat
    29161 taggcttaat gttctataat gattacactt ggggttcttt tgttattaat ccaggagtgg
    29221 gtgctacaaa tgatggcgga caggttatgt ttaccgaaat acagattact ttcccaccta
    29281 caggtgatgc agcagaagat ccttctgtaa gcccggttac caatatgttg aatcaaaatt
    29341 atcccaatcc cttcaatccc gagaccacta ttactttcga tatgccaaaa actgctaatg
    29401 ctaatttatc cgtttataat gtaaagggac aattagttaa gacccttcat aatggagttg
    29461 caaattatgg cagaaattct gtggtttgga atggtacaga taacaatggt tctcaaatta
    29521 gcagtggaat atatttctac cgtctttcca ccgatggaaa agttgaaact cgtaaaatga
    29581 tgttgttgaa ataaaaatag ccttcctcaa taaaaataag atgaagccct gggagcttat
    29641 tccggggctt cttcatttaa agaactacat tctgtcgttc atcttacaac caaattatgt
    29701 tgttttcgtt atattctata aaattgttgc acatatccaa ttatctttcc taattattaa
    29761 tcaacctttt gagatacaat cacttccaat atatagtgga ttgttcaaca agataaaaaa
    29821 cacattaaga tcgattattc gaagctatta agatttaatt agaccaagaa tatcagcaaa
    29881 atacaaagtt aaaccttact ttttcttgac agttaacaat atacaacttg ttatccaccc
    29941 aagcatttct cctccttaac tatgggttga tgaggagagg aggaggagag gaggatgggt
    30001 tgtttgtttg aggcgtccta aataaagtgg actaaaatta tggagaaaga aatgcacaaa
    30061 aagtctacag aaaaggaagc atattcaggg catcaatgcc ttcgaacctt aagttgctca
    30121 taactgctta atctgaggga attattttcg gtggcttcgg tggctaaaca ttcttctaaa
    30181 atccttgttt aagaaagctc aattctctca atccttaaat ccttacatcc ttaaattatg
    30241 gtttctaagg ctaaacctac gaaaattgcg taatctgaat gaaaatcttc ttgcggaaga
    30301 acgacatcct gttgtttcac ctcatagtca aaattatata gtttccgcta tattccatta
    30361 tagctttata gtttgtctgt tgtggattga acaagagatc aaacttccag agttctagtt
    30421 attttatagt tacaagttct tatctgcgaa acacagatag ttatttagaa agatggcaaa
    30481 aaaatcagtt atcccttttt agcaatagcc acataaaaaa tgggcaacca atgaaagaag
    30541 taataattcc taccggtaat tccatcgaaa gtaagtgttt agccagcata tcacaagcaa
    30601 gtaaaaagat ccctccagtc cacaacgaaa gcagatatat attcttttgt ccagatggaa
    30661 caaaaaaccg aatgatatgc ggaataatca atcctacaaa accgataata ccagcatagg
    30721 caacacttat accgattacg atggaagtta gaacaaatat ttgtttcctt agcgctaaaa
    30781 catttattcc aaggctcgca gcatagagat ctccgttact taaaatatcg atagcagtag
    30841 atttgaaata gagatagatt aaaataagca gcgaaaccga gaaaagaaaa agaaagctaa
    30901 tccattcatt tttagtgaag atatgtccta aattacccat cagagtgccc aaaattaatg
    30961 ctgtatcttg ttggtgtaga tacataatta acgaaatacc agaagcaaaa aacatcccaa
    31021 caatcacacc cgcaatcaat aaacgagttt tatcaaatct acctctatga tgtgccaatt
    31081 tccaaacaag caacattgtt aatcctgcac cgataaagcc tcctatcggc atcaacataa
    31141 taaaaccggt tatcccaagt acaatacttc caaaagcagc accggatgaa attcctaaaa
    31201 tatagggttc ggctaaagga ttagcaagca ttagttggta gatagagcca attccagcaa
    31261 gagccatacc ggtaaaaaga gttaatagca agcggggaat tctcacttga gtgataatat
    31321 ttgtatttgg tttacctata aagagatatg ctatgataac tgctaaacca gttaaaaaaa
    31381 ataagatcca aaaaagcgtt tttttcattg ctctttccgc caggaatcaa aaatactggc
    31441 taattgcttc attccttgta tacagcgggg agtggctcgt tgaataagat cgggatttat
    31501 ctctttctcg aagtatattt ttctattttg aattgcagga atattcagcc agcccttacg
    31561 gttgattata ttttccagag tatcctgtga gtagcaaatc ataatatcag gtttggctgc
    31621 aataacagcc tcaggattaa ttctggcata atctctttcc agtgtgggaa aaatattatc
    31681 gccaccagca gtttcaatca gttctccaat aaaagaagca tcggaaacgc tcatcaaagg
    31741 atttcggtat atttccaaat agacttttgg acgagtttta cctatagaat tctcttttat
    31801 atttaatatt tcctgtttca tctggcttat ctgttttgtg gcttgttctt ttctatcgat
    31861 taatatgccg attctttcaa tagctgaaag catctccata atgcttttag ggtagattac
    31921 ttctacggaa taccccaaat tcttcaaatc taacgctaca ctttcttgct ccagagaact
    31981 gcaaaaaaca agttctggat tcaaagcaat aatggcttct ttatcgatga cgctaaaaga
    32041 gccaactacc tttttttgtt gaagagagga aggataatta cattctgcag ttattccaat
    32101 aatatcctct tcagcatcaa gtgcacaaat aatttctgcc acttctggag aaagaacaac
    32161 gattcccttt ttggctttgc tattcatttg cttgcaggaa aaaacaaaca tagctaaaat
    32221 aattaaggct acatatacta tttttttcat taatttattc ctcgtgcttt gctcatatta
    32281 ggatcaggaa atattataat actcagtaaa aactaaatag aactgatccc ccttttctgg
    32341 aaaagtaaga tcagcacaaa ttacgcatat atcagttaag ctgctataat atagcatcct
    32401 tatttgtaac actatctata gctaaaatga tattagttta ccttatggca taataacctt
    32461 ctcctgtcaa ggaaaatgaa ctttggttta aatttagata tccgccatac tctatttaaa
    32521 tttaattaaa agatggtttt ttcataataa ctaagtttat aaatgcatta agtgcttagg
    32581 tgaaaatcca tactttatgt tcagggttta tgcttgattt aactcctaat cctgaaaata
    32641 gaaaagccta ttaatttgtg tttatgtaat gatagattta aggcaattgt tagttatata
    32701 attatatagc ataattgata gttaagacag gcaatagttt tatttgcaac aatctaagta
    32761 ctgttacctt ttttgcttga caatcatttg caatggagta aaaaggtttt agtaaaaata
    32821 aagcgatata ggaaaacaac aataagtcca cataatttaa atcctaacag gaaacgaggt
    32881 gaattattat aacttccagt aagatatggg atgccatagc tttaataata gcgttattaa
    32941 tcagcttagt gttgattgca tccctcactc aaataatcgt tggaggctta gttttagaaa
    33001 ctttcagtaa agttactata cgcatcatca atatcctgtt tggtatctct atagtattaa
    33061 tcattcttat cgtagttatg gaaaatggtt ctccagcaac aacgatggca tggattttgg
    33121 ttctaatctt tttaccaata attggcttta ttttgtattt gttttttggc agaaattggc
    33181 gaaaggagag gttattcagc aaaaaggatt atgctgatac tcagttgctg gctacctata
    33241 ttcaaaaata tcctttaacc atagaagaca attggcaaac agaattgagt gcaagattaa
    33301 ataagctttt aaagaacaat agtaaagcta cccttacagt aaataacgaa gttaaattat
    33361 attccgatac gatggaagct tttaacgcaa tcctgaaagg tatatcctct gcaaaacact
    33421 ttgttcatat ggaatatttt tcaatttttt ccgatgagac aggttatgat atgcagaaaa
    33481 ttcttatcca aaaagccctt gagggagtgg aggtacggtt tatttatgat gatgtaggtt
    33541 gctggatgct gaaaaaggaa tttaaacagg aattacataa tgcaggagtt gaatttgttc
    33601 cttttatgcc ggtatggatt ccctttctga acagtcgtat aaactatcgt aatcatcgta
    33661 aactggtaat tgtagatggt aaaaaggcat atttgggggg attgaatatt ggtgataaat
    33721 acttgggaaa aaacagctat tatggttatt ggcgtgattc cttagctgaa attgaaggac
    33781 aagcagtact ttctttacag gcgatttttt tagcagactg gaattttgtg acgggaaaga
    33841 acttgatgca ggaaacctta ctggataaat atcttccgac agatattact gttaaggaca
    33901 aatttctacc aatgcaagta gttgcaaatg gtcccgatag cgattatgct agtattatgc
    33961 aattatattt tgcagcaatt acatatgcga aacataatat tcgcatttct actccctatt
    34021 tgattttgaa cgaaactctg ctatccgctt taaaaattgc tgcaatcagc gatgttaaaa
    34081 tacaaattat tatgcccgat aaaccggatc attttttggt gttttgggct tctcgaagtt
    34141 attttcaaca acttttagat gtaggtgtgg aaatatggac ttatcataaa ggctttaatc
    34201 actccaaaat actaattgta gatgatgaag ttctattaat tggaactgtt aatatggatt
    34261 tgcgtagctt tagccataat tttgaactgg ctgctacaat atatgataga aatgtatgtg
    34321 cagaggcaat aatgcatttt gaagaagacc tgatgaatag cagtaaaata aatgcggaag
    34381 aatttaagca gcgtagtatg ctgtataaaa ctcgagaatc aatatgtaga ttggtttctc
    34441 ctttattata aagggagaaa tatgtgcaat attataagga gaaaaggcta ttaaccagaa
    34501 aggttgatat tctatcaatc ctgaaactaa cttatccagc agccaaaaaa acataggtga
    34561 ctaaatagct aaagtgttta tttttaatag attattatag gatagaagat catgaagttt
    34621 atcagttatc ccttatctcg aaatctggac agaatagttc aagaactggc taagccaaat
    34681 agtattcttg tttttcccac tttaattgct gcccaaagag ctgagcgcga atatttacga
    34741 aattgggatc tttcccggtg taatttttat agtgtggaag aattgcgttc tgcactaata
    34801 ttaccccaat tgcccatcgt gacagatgaa aaaaggctac tgtgtctgta tatggttatg
    34861 gaagaaagtg agcgtgattt ttttcatatc tatagctatt ccgacatagt ggaatggggt
    34921 aggcgttttt ttgatttctt tgaagaactc tgtgtagaat gcattgaaat ggaaactttg
    34981 gaacaactac ctaattccgg aattatgagt atgcaggaat ggcaggaaaa atatctggaa
    35041 aagattatag ctatccgtaa taactacttt caatttatta gttctttagg tttcagcgat
    35101 gccatttttt atcttaaatc cgaaaatatc attgtgccct ggcagggctg tgaggttatt
    35161 tatatcaatc aatactatta cagtgctctg gaaaaaaagc agatagaagc tcttgaagat
    35221 gctggaaacc aggttactat cgttactcac ggtttggaaa tttcaggcga aacagatgac
    35281 tggaaagtgc aggattttga tttgaaaaat gcctggaatt cgcttgagcg caaacctgtt
    35341 atagaaattt tagaaactga gaatgaaact cagatggctc tgtcgttcct atcctggaat
    35401 atggacaata attgtcctaa cagcgctatt atagatagca gtttccatct aaaaagttac
    35461 agtcatttct ttcctgaaga acattttgct aaaccggata attatccttt tagcgatgga
    35521 actatctata aaatgctatc tgcaatatat aaagggttga aagcatccaa agaaagcgat
    35581 ggctatcttc cagtaagaat tctggctaat tatatatccg aggattggtt ttgccactac
    35641 ttttttgagg agaacagtaa tctggaaaga gaggattata tccaacagtt gcgtttggag
    35701 cttagtgata taattaataa agatactctc tatgtaaatt gtgctttttt caataacatt
    35761 aataagccgg ttttacaatc tctggtgctt cagtataata acttaatgga agcttttaca
    35821 gaagcgacaa atctatctga aatctgctct ctaattgata gccctaatgg tttatgccta
    35881 attaaattgc tgcaagaaca cgaaaagcag tatacagata ttattcctgt tttctggaca
    35941 caaatggcca actttatggc tatagaaaac ttggggttaa taaactcctg gaaacagatt
    36001 tttgaggaag acaccttggg agagaattta ctggatctgc tgcttaacta tctaaaaagt
    36061 gctaagataa gtatacaaag gaatgaaaag ctatctcctg aatgggaaat aagcaatcta
    36121 ctggattcgc gtaatcgggc tattaccaaa gtagctttct ttcaaatgat tgaaggcatt
    36181 gttccttcca atccaactcc cgtctggtta tttaatgagt cgcaacgggc aaaactgggg
    36241 ttgaaaactt ataacgatgt tagaaaatgg gagcgttatt atttcttccg tcttttgcta
    36301 tgttctgagc aggcaatttg tttaagttat gcgaatcagg aacgcgatat tagccctagt
    36361 tcctttttag gagaattgga agaactatta caaaatgaag atgttagttt cttgcaaaaa
    36421 aagatggtgg gtgtttcttt aactgaagta tatagtgaga atactccagt taaaactatc
    36481 cctgagcttg agaataatga atattgctgc gtggaaaaat tgccagagga ggatttcttt
    36541 atcattcctt ccgatccgat gacagatttt cctaaaagca atattcgttt tagtgcttca
    36601 gcacttatcc agtttatgaa aaatcctttt ttgtggtatg tggaaaacaa atgtaaactc
    36661 tatacacaaa attgggaagc tgaagaaaca atcagctata aactcttcgg caatattatg
    36721 catagctatt tttctacaac tctctcaaaa ttgacaggtc gccatatatc tacagacgct
    36781 ctggaagcat tttttggaaa tagtgctaaa ttggaagctc aactgcgaga agtgattact
    36841 tccgataaac ttatttacaa gattcccaag aattacaatg cggagttctt gggagatatt
    36901 ttggccaaaa aattagcaca atctctttac ctattttata aagactggct gaaaaaacag
    36961 gttgaatacc gcacttttga tctatatcca gaaacggatg atgactcaaa ccaagtggta
    37021 gaatataaaa aattgggcac tgtctcattc caggaaagag attatttact ttatatcgga
    37081 ggtagagcag acctgcgtgt ggaactggat aataaagcaa taattgtaga ttttaaaaca
    37141 ggcagccacg actatcggca gctctgtatt tatgagtggt actattatct tttggatgag
    37201 attttgccag cggataatgt ttcatctcta ttttggaata tattagaccc cactaaaaaa
    37261 acggaaggtg tgaatgaaga aaaacggcaa aagctgaaag agcaaatatt tgaacacttt
    37321 acctattgtt tggctaatgg ctatagcaca ataactaaag tttctgatag acagcgttta
    37381 cagtatatta cccgtgctga tctactcaga gaaataaggg gctttgaatg attgaaaata
    37441 actgccgaat aatttgtgcc agtgctggaa ctggaaaaac ctatcgtctc tctttagaat
    37501 atcttgcgct aatcctgaaa tattatggaa aaagcgaatt ctctttagat aatatcctgg
    37561 ttctaacttt cactcgtaag gcaactgctg aaatcagaga acgaattatt aagcatttgg
    37621 aattgctact ctctgataat gtagaagata tggatgaaca aaatatattg ctaaataatt
    37681 tgcgagaact gatggaggaa agatttgtcc ctttaagtga aatagaaaaa aatatgctac
    37741 tgagtgcccg caaagaacta atctgcgatc aaagccattt acaagtgatg actattgatt
    37801 cttacactgg acaaattttt agaaatattg tgcgtcctat ttgtaatata gaacactatg
    37861 atattgatac cgaagcgatt attaaaataa tgccgttatt gttcaaacat ctaatgaaac
    37921 ccatattccg cgaaagctta caagatttat taagcaagaa agttagccgc tcattggatg
    37981 attatacttc cttcttccgt tctttaattg aacagcggtg gctatattat ctaattacca
    38041 agcgaaaatc tacccgggat agttcctcaa atggaatatt gatgaattta gaccccgatg
    38101 ccgatagcac agaatattat gggaacttcc tgaatgctat gcaggaactt attcagatag
    38161 tggtaaatat ttgtgctgaa aaaaacgaag cctccttggc agattatttt aacaaggaat
    38221 tcaaacaact ggtgaacata gaatccaata caattaatgc tatatatata atggataatt
    38281 tgaaacgctt ggatttagat gccgcagaaa aactacttac aattctggat aaaagcaata
    38341 tctggaacaa gcagaaaatc agtgctaaaa aatatactgt agaaaatact caactgcaaa
    38401 atgcgcaaga tatggcgaaa ctctttttgg cggattattt gatcctgaaa ctatatattc
    38461 ccgaacaaaa gcaaattttg ggaatttgga gtgatgttct gaaagaatac gatcgactaa
    38521 tctaccgcta taaaaatctt acctataatg atataacctg gttaactttt gaagcccttt
    38581 tcagcaatga accccctttt ctaaatcctc aaagtgctga ttccgctact gaattttatc
    38641 agtttttaac ccatagaact cgtttcctgt tgattgatga atttcaagat acctccctta
    38701 ttcaatttaa tgtgctaaaa ccaattattg aagaaatcac tgctggagaa ggttctaaac
    38761 cctttggtgg cgtaattgtt gtcggtgatg aaaaacaatc tatctttggc tggagaggag
    38821 gtgaacgcga tttattgcta aatttgaaag atatttttcc tgccctgaaa gaagtgaaaa
    38881 tggaatcctt aaaacaaagc tttcgctgtg gacctacttt gatggaattt attaatcagg
    38941 tgtttagtaa tagcgctcta catcagcttt tacaagaaaa agagatgcaa tggaattatg
    39001 cccgaattga aagtcaggaa tacaaaaaag aagaaggctc ggaaattgaa ttctgcctgc
    39061 gcaatttatc gaaaagtgcc gataatacca ataaagaaaa agatatttat caagatttta
    39121 tcgaatgtat ggttatgcct gcgctgagaa aagatgaaac cggttcaatc gctattttat
    39181 gcagaaccaa taatgagctt accaaaattc aacatgtttt agatgaatat aaaataacca
    39241 gtttatatca acccaatagt tctattattg aacatcacta tgttgctcca ttactttcat
    39301 ggctgcgttt tcaggcttgg ggtgattgga tggattttat tgcttttctg cgttcggatt
    39361 atgtgttaat caataccaaa gtactaaaac aaactttggc tattatcgct cgaactctgg
    39421 aattgcaaaa ggaatgggaa aagccaatcc taccggattt ttctgcttta cctataataa
    39481 atactctatt ccaactgtcc ttgcaacaac aggggaaaaa agctaccgaa atatgcagag
    39541 atttgattga cttctgtttg ccttccaaac aactaagtga acgagattat ctaaatctgc
    39601 aacgctttct ggatattttg gctacccgta tgttgactga tcccgaaaaa ggaacttcct
    39661 ttcctgactt gcttgcctac tttaatgaaa atgctaattc tgagaatttt caacaagtat
    39721 ccgtaaatat cagcgacagc ttacaattgc ttaccttcca taaatccaaa ggattgcagt
    39781 tcaaaagagt tttcgttttt tataattttt acggtaaaca tagagacacc agcgatacta
    39841 tatcctgggc tttaagctat gaagataaaa cctttaataa aatcagggat tacggaataa
    39901 gtttgcacta ccagaaaata atgaaactat caagctatgc acctctctat ctacaagaac
    39961 aaaaaagaga acaattggaa gaaatgaata atctctatgt ggctatcacc cgcgctgaac
    40021 aaaaattgca cttatatttt ggctttcaat ataaagaagg ttgggatgcc tattacgata
    40081 aatataaaag tgagagctta ccggtcgcta tttgcaatgc tgttcaggaa atctttatga
    40141 aatctactcc cgatgaacgc ggtgtttatg tccaaaaaag tatgtttgaa ctatgcgaga
    40201 aagataaaac ggaaaaatcc gaaagaagca aagccataag agagcaaatc aattcttcca
    40261 taaaaatctg tcatcataga ccttttgatc ctgcaaacta tcttcctgca gaagttccag
    40321 atataaaaga cctgaaaaaa tattatctaa ttgagcgccc taacctgatt ggagatttac
    40381 tgcattttta tatgtccttt attattcgga atgagctaaa agaacatcag tatgcattgt
    40441 atagatgcct aaaccgctat ggagcaatct ttacaggttc tcaaatagat aattttgctg
    40501 cccgctgtaa acaagtctgt gaacataatc cttatctatt tactaccaga tggaatgaga
    40561 tttttacaga acaggaaatt gtggagaata gtaaaatatt cagaatagat cggttgatga
    40621 ttaatactgc tgaaaaagaa tcactgatta tagattataa aagcggagat atacacgatc
    40681 caaagcagct tataaattat aaaaacgctc tgctgaagtt gcctgcctta caaaattacc
    40741 agattgatac taaaatagta cccctataat tgtaaagtgt caacactgct gaaatagctt
    40801 ataatatctc tgttaatcct atgtaccaga taaaatacag caagataaag aaattgtatc
    40861 gttggctgtg agtagatgag gacatcaacc gtcccgggaa atggcctctg ctgactcttt
    40921 taaacaaaag tattgactaa atctgcaagt ttatagaaaa ggataaatta gtataattac
    40981 ctaaaggaaa agcaatgcta aaagcgaaaa tttatgtgaa actaaaagcc agcgttcttg
    41041 atccacaagg caaagctgtt accaattccc tacataattt gggttttaca gatgtcttgg
    41101 aaacccgaat cagcaaatat atagaaatga attttacaag tgacgatagg gaaaaggtga
    41161 gccaggaagt agaatatatt tgtaatagcc tgttagctaa tcctaacact gaagtatatc
    41221 aatatgagat agttccgttg gagcaaaatc ttgagaatta gtgttgtaac atttcccggc
    41281 tctaattgtg attacgatgc ctatagagtc tgccttagca gaggtcacca aacaaaactc
    41341 atctggcata aggatacaga cttggctaat cctgatttgg ttattcttcc agggggcttt
    41401 tcgtatggag attatcttcg tgggggagct ctggcaaggt tttctcctat tgtgaatgaa
    41461 gttatttctt ttgctaagaa aggtggttta ttgcttggta tttgtaacgg ctttcagatt
    41521 ttaacggaat gtggtttact gccaggaaca ttattaatga acgaatcttt actatttatc
    41581 tgtaaacatc aatatatccg ggtggaaact gaggccagtc ctttcaccca aagtattggc
    41641 aaaggaaaag ttctggatgt tcctatagct cacaaagaag ggaatttcta tacagattct
    41701 gaaactttaa agcaattaca agataataat caaataattt tccgctattg cgataagcaa
    41761 ggtgagatca atttacaaag taatcccaat ggctcattag ataatatagc cggaatttgc
    41821 aacccgcaga gaaatattct agggatgatg cctcatccgg aaagatcagc taccgatacc
    41881 gttccgactc aagatggaaa gtggattttt gaagcaattg aaaaagctct ggaataattt
    41941 tctggaacta attgttcctc ctgcatgttt agtctgcgga aatagaattg aagaccgcac
    42001 tcaggttatt tgtgaatttt gcgaagccaa gctttcttta ataggagaag gaacctgtcc
    42061 tgtttgtggt agcgaaaaca aagaactacc ctgtgaggtc tgtttggatg gtcaatttga
    42121 atttgattcc gcacgctcca tatttcgtta tataggtcct atacgagaac tgattcacat
    42181 tttgaaatat gagggctata cttatcctgc cggttatttt gctttaccga tagcggaatt
    42241 aatagaatct gaaccaagct taaagaatca cgattatata tgtgcggttc ctttgcatag
    42301 agttagaaaa agggaacgcg gttataatca aaccgatttg attgcttatg ctgtttcgaa
    42361 actgatttgt atgccctatt tgaatcctgt tacaagaaga ataaacactt ttagccaaac
    42421 tctgctctcc aaggaacata gaattgcaaa tttaagcggt gctttcactg ttaaacataa
    42481 agaccaggta actggtaaaa aaatcattct gatagatgat gtttttacca ctggcagtac
    42541 tttaaatgaa atagctaaaa ccctgcgtgc tgcaggggct aaaaaaataa gcacgattac
    42601 tgttgcccga gcttgaagat gaataaagaa tacttggaaa tccgaccgga aacttcagaa
    42661 agcgaaatta acgagatgat tgaaaaagta gcaaaattca tagctgaacg acatttggct
    42721 ccagcaggaa taatgttttt tgaatccatc cgtcctttac acggaattgg cagtcaagcg
    42781 ctatttatga ttttacctat agcggagatt attttcgatt cccgaaagta ccaacaattt
    42841 gctataatga tggaagacga aaaaaacttg aaaaaactga tcagtcgtat ggatgaactg
    42901 gatgaagaat ataatagaga acgcagaaaa gaaaatcgat tgaaaagaca gcatcgaaaa
    42961 gccaaaagga aagcattctt tagaaaaata tttaaaacca aaaaataaaa ttgcttgaat
    43021 aagtggagga tatatgcaat acgatgaaaa acgccttaca cgtattgttg caactgattg
    43081 tggcagcact accaccaagg caatattaat cgaatgggtg gatggagaat ttcgtcaaac
    43141 ccttcgcggt gaagcaccta ctactgtaga agcaccctta aatgatgtta ccaaaggtgt
    43201 tatcaacgct acccaagaac tggaggaatt agcacgccta aaatatcata atcccaatat
    43261 taagtttatg gaaaatgggg aatttgttat tccgcgcaaa ggggatgtag gtgtagatgc
    43321 ttatgtatca acttcttctg ctggaggtgg attacaaatg atggttactg gtgtggttgc
    43381 tgctatgaca ggagaaagtg cagaaagagc agctttggga gcaggggcaa ttgttatgga
    43441 cctcattgcc agcaacgata gaagaatgaa ctatgaaaaa attgaacgca tccgtcaact
    43501 taggccagat atgattttaa tggctggagg tgaagatgga ggcactgtaa aacatgttgt
    43561 agaaatggca gaacttgtag ccagtgctga tcctaaacct cgtttgggct ccggttataa
    43621 actacctgtt atttatgctg gaaataaaga tgcccgcgaa gaaattatta aaactctggg
    43681 cgagaaagta gacctgataa ttaccgataa tattcgtccc aaactggaaa ttgaaaacct
    43741 cctgccagca agggaaaaga ttcatgacct atttatggaa catgttatga aacaagcacc
    43801 tggctataat aaactgatgg aatggactat aggccctgaa catgaacaag ttcctattat
    43861 gcctactcca gccgcagttg gaaatattat gcaagcaata gctaaagaag aacaaattga
    43921 agttttagga gttgatattg gaggagctac aaccgatatc tttagcgttt tcactaaaga
    43981 ttttgttttc aatcgtaccg ttagtgctaa tttgggaatg agttatagca tttccaatgt
    44041 tctggcgtca gcaggattgg ataatattat gcgctgggtt ccttttgata ttaacgaaaa
    44101 tgaattgcgc aatatgatta aaaataaaat gatccgtcca acaactgttc cttctttgtt
    44161 ggaagaacta gttttggaac aggcaatagc taaagaagca ttacgtttgg cttttgaaca
    44221 acataaatcc tttgccagtt cactgaaagg tatgcagaaa cagcgtgata tttctgaagc
    44281 attcagccaa tccctttccg gagaaaccat tgttaatatg cttactttag accttttagt
    44341 aggtagtgga ggagttttat ctcatgctcc tcgcagaaat cagaccgtga tgatgttaat
    44401 tgatgctttt ctaccagaag gaattactcg tctttctgta gacagcattt ttatgatgcc
    44461 tcatctgggt gtgctttccg agattagtac caaagcagct actgaggttt ttaacaaaga
    44521 ttgtatggtt tatttaggaa cttgtgttgc ccctgtaggg aaagggaaaa ttggtaaacc
    44581 agcactttat gccaaattag aacttcctga tggctcggtt tttgaagaag atataccctt
    44641 tggtgaaatc cgtttaatac cctgtgaagt tggtcaaaag gcaaaagcga ctcttaaaac
    44701 ccacaagggc ttgattttag accaagataa gaaaaaagaa attaccgttg atcttcacgg
    44761 tggaatagtt ggaatcgtga tagatacacg aggacgtcag ccatttgttc ttcccaccga
    44821 aaaatcagaa cgcattgctg ctctgaaaaa gtggatgaaa gaattgaaag cataccctga
    44881 acatatctta gtataggagg aaaaaatggg acaagcatat tccgctggat taaccgttac
    44941 cgataatata atcttacgaa aagaacgcat tctacccctc aaagggcagg tagttgttaa
    45001 aaaaggtgat aaagttaaag ctgaagatgt agttgctgaa actttattac ctggaaaagt
    45061 gatacctttc aatctggcta ataaattggg tgtaactccc caacaattag aaaattacat
    45121 aaaaatccaa cccgggcaaa agattacgaa aaacacagtt ttagctgcca ataaaggtct
    45181 ttttgggctg ggaataatga aaaatgaagt tctttctccg atcgatggag aagtagaaaa
    45241 tatctcttct gttactggac aaattttact gcgcgaacca aaaatccctg ttcaagtgaa
    45301 ggcatttatt gatggattag ttactgatgt tattgaagat gaaggtgtta tcatagaaaa
    45361 taaaagtgcc tatatacaag gcatttttgg tttggggggc gagacctatg gcgaattaaa
    45421 aatacttgca gaatctccgg atgatgaatt ggatgtagct aaaattgatg cttcttgtgc
    45481 aggaaaaatt attgttgcag gtgcctttgc tgcttatcat tcaatagata tagctcgtaa
    45541 aaatggcgta aaagcaatta ttaccggtgg acttgatgac caggatatta aaaaattact
    45601 cggttacgat atcggggtag ccattaccgg acacgaaaat atcggtataa ctattgtttg
    45661 cacagaaggt ttcggcaaaa tcaatatggc taataagacc tttgaacttc tgaaaagatt
    45721 tgagggacat aaaacctcta tacatggcag aactcagatc cgcgctggag ttatcagacc
    45781 agaaatcatt attcctctcg agtttgatga aaaagaactg gtcgctaaaa caagctctat
    45841 gcctgtttta gaaattggaa cggtgattag aattatccgt caaccgcatt ttggtcagat
    45901 tgccaaagtt acagcacttc cagaagaact taccaaagtg gaatcggaaa ccctggttag
    45961 agtactggaa gcagaatttg aagatggaac aaggacaata ctacctcgtg ctaatgtgga
    46021 agtgatagaa tcataggagt ttaaacaaac tcgtaaacaa cataactaag atccaagttt
    46081 tgcgtaaccg atgtgcaata ttaaagggat gtcctctgct cggtaaacgg aacttgatta
    46141 tataaaatgt agcctttcag gaatatttga catttcccca aataacttga gggggaatgt
    46201 catttttcct tatcaaccca acttttttta agagtttctc ttttctaaga tactgcctag
    46261 tatgccattt ctggaaatcg ggttgtttct tgcttggttc ttgtggcgca attgccgtgg
    46321 cactaatggg gaaattgccg atcagttcca ctgcgtctcc agcgctgtta ttaaggaaga
    46381 acatagatat atgcagcgaa aattgtaaca ttacgaaccg caaaatctgc actcgcaaaa
    46441 cagctaagta tctataaata atagttgttc agcttttaat aatcacctaa ttattcctgt
    46501 aaaataagca catatttgtt tcataaccag ataaatattt gcaaagcagg gtttataaag
    46561 cacaaatgaa gttcttttgc attgacaata ttggcacaat aaaggaattg gcttttttta
    46621 agtgaaataa tataatgggt attatgtctg ctgagaaaga aaaaacagat accctaagtg
    46681 ttcagtatgt aattttggag aaaagtaatg agcaagaata acaaaactgc aattacccct
    46741 actcgggaag aaaattatgc cgaatggtat caacaagtaa taaaagcagc cgatatggct
    46801 gaaaatagtg aagttcgcgg ttgtatggtt ataaaacctt ggggctatgc tatttgggaa
    46861 aatattcaac atattctgga tagaatgttt cgcgaaacgg ggcatagaaa tgcctatttt
    46921 cctattttta ttcccaaaag cttttttgaa aaagaagctg aacatgttga aggttttgct
    46981 aaagaatgcg ctatagtaac acatagtaaa ttggaagcag atgaaaaagg cggattaaaa
    47041 cttgccggag aacttactga accttatatt gtacggccta caagcgaaac aattatcggt
    47101 gcatcctttg ccaaatgggt taattcctat agagatttgc ctttgttaat aaatcaatgg
    47161 gctaatattg tgcgctggga aatgcgtact cgtctatttt taagaaccac tgaatttctt
    47221 tggcaggaag ggcacactgt gcatgaaaca gaaattgaag ctcgagaaga aaccttaaaa
    47281 atgctgaatg tttatgctac atttgcgcgt gattatcttg ctttgcctgt cattcaaggt
    47341 gaaaagactg aaagtgaaaa atttcccggt gccgttacaa cttattgtat tgaggcaatg
    47401 atgcaggata gaaaagcatt gcaatcgggc acttctcact ttttgggtaa aaactttgcc
    47461 aaagcctccg aaattaaatt tcaaaatcgg aacggagaaa tggaatatgc ctggactact
    47521 tcttggggtg tttccacgcg gttaattggt gccttaatta tggcgcatag tgatgataat
    47581 ggattagttt tacctcctaa aattgctcct tcgcatattg cgattatccc tatttacaaa
    47641 aatgatgagg aaaaacagat agtgatggat tttgccataa aagtgcagaa tatttttaaa
    47701 ggattgtatt tggatgatat tcaaatattt actgaaatgg atacaaggga tatgaccagc
    47761 agcgaaagaa attggtattg gataaaaaaa ggtattcctc tacgcctcga aataggccct
    47821 cgtgatattg ttaacgattc tgtaatgctt gccagaagag ataaagaacc taaagataaa
    47881 cactcaatta aaattgataa tttggtagat tatgttctaa aaaccttgtc cgaaatgcaa
    47941 gagggctatt atcagcgggc tttgaatttt agagataagt ggacagtaga cattgatgat
    48001 aaggaagaat tctatcggtt ctttaattcc aaaaatgaag aacaggaaat aagcggtggt
    48061 tttgccaatt cccactggtg tggaaatcaa acctgcgaag aaaagataaa agatgacctg
    48121 aaagttacga tccgctgcat tccttttgag gcaaaagaag aagaaggcaa atgtatctat
    48181 tgtggtgaaa aatccaatcg tagagttatt tttgctaaag cgtattaaaa tgaatgcagc
    48241 catcatcaat attggcaatg aaattttgat gggtaaaacc atcaatacca atcttgccta
    48301 tttgggttcg gaacttacca aattgggaat attagcagaa tatgcagtca cgattaaaga
    48361 tgaaccaaac gccataaaac aaacattgca agatacctgg aaaaaatatg atatagtgat
    48421 aactactgga ggtttggggc ctactgaaga tgatttaact aaagctgcaa ttgccaattt
    48481 cttcggtaag aaccaacact ttgataatag tatctgggaa cacatccaaa aaatgtttgc
    48541 tttccgaaat atcccaatac cggaaaccaa tcgttcccag gcaatggttc ccgatgattt
    48601 tattcctttg caaaatgact taggaacggc tcctggcttg ctttatcagg aaggagatta
    48661 tttattcttt gcattgcaag gtgtcccctc cgaaatgaaa catatttttg aaacccatat
    48721 aaagaaaata atagcaaaaa catacccaaa ctataaacct ttgcatataa aaacattgcg
    48781 aacttacgga atagctgaat cccgattggc tgagttgttt actctgtctg atttacccga
    48841 aggaatatct atagcatggt tacctcaaat tggaagagtt gatttgcgtt tttatggaac
    48901 tgatgtaaga aaagtggaag aagctgcaga tagcgctcta aataaaatcc atccttatgt
    48961 ttggggttac gatgaagaaa gccccgcaga aattttatta tctctattgt tgaataattt
    49021 ctacactcta agtgtggctg aatcctgtac aggtggctta gttcaaaagc tagtaactga
    49081 tgttcctgga gcatctaact ccttcttggg tggtgtaata acatacacta acgaattgaa
    49141 gaaaaagata ttaaaggttt ccgataaagt tttggaaact aaaggagcag taagcgaaac
    49201 ctgtgcaatg caaatggcgg agggaataaa actattgaca aattctacct gtgcgatttc
    49261 aataacaggt attgctggtc ccgacggtgg aacagaaaaa aaacctgtag gaacggtgtg
    49321 ctttggcttt atagccgctg agaaacattg gtctgaaaaa caattcttta ctggagatag
    49381 ggaaattgtg cgcataaaag ctgcagaatt tgctattctt accttaatca aatacttaca
    49441 aggcaggtat aattgaatat cttagttata ggtagcggag gtagagaaca ttcgctagca
    49501 gagtgttttg ccaaaagtaa aaaggtggct aaagtatatg tgtcaccagg taacgcagga
    49561 attgcgaagt actatgaatg cgtgaaattg ccaaagcagg aagatatatg taatttttgc
    49621 cgtcaaaata caattgacct tgtctttatt ggaccagaac agccaatcgc tgagggttta
    49681 tctgattatt tatgggaaaa aggaatatcg gttatagcgc ccagtaaatt tgccggtcaa
    49741 ttggaaacca gtaagatttt tgccaaggac ttaatgcaga aatcaaaaat tcccactgct
    49801 agataccaaa tagcgagaac tgtagaacaa gctttggaat tgatcgagaa ttttggctat
    49861 cctgcagtta tcaaagcaga tggattggca ggtggaaaag gggtaataat agtttcggat
    49921 ttaaacgaag ccgaagatgc attaaataaa ataacaataa gtgcaagagg agtaattgtt
    49981 gaagagtttt taaaaggttg ggaggtttct ttatttgcat ttacagatgg ggtaaatttt
    50041 aagaccactc tttttgctca agatcataaa cagctatatg atggggaccg gggtccgaat
    50101 acaggtggaa tgggtgctta tgctcctgtt cctgaggccg aaaaatatcg aagcaaaata
    50161 gaaaaagaga ttgttgaacc gactctaaaa gcaatgcgtg atatgggata tccatataaa
    50221 gggattcttt attgcggatt gatgataacc agagaaggac caaaaatagt ggaatacaat
    50281 tgtcgtcttg gagatcctga agcacaggtt atattgccat tactggaaac agacttggtg
    50341 gatatctgcc aggccatttt gcagagtgaa ataaataaaa ttgagcttaa attctcggat
    50401 aaaactgctt taggagttgt tttagcttct ggaggttatc ctttaagtta tcaaaagggc
    50461 tttcccctca ttatgccttc aaccctttcc ggtatttatt tttctggagt agcagatgct
    50521 gaaaatggcc ttgtaacttc tggcggacgg gttttatgtg ctgttggtgt tgggaaagat
    50581 ctaaatgaag ctagggaaga ggcatacagt aaagctcagc aaataacttt tgaaaacagg
    50641 atttttcgac aagacatcgg tttaaggaat aataccttat tagacaatgg agacaaataa
    50701 tattatgaca atatactcta ttatacaggt taaaaacctg tcccttgcca agttttataa
    50761 ggcaggttta ttattctatc ttagtttttt cttacctgct cttatatttg cctcccctat
    50821 aacacttatt tctgaaagtc aggatttact tactattcaa ttcgaacttc cagaatatga
    50881 actggaagat gtaacaatta atggccaaac ctggaaaaga ataatctgta gtgagagttc
    50941 tatttatggt gaagaaggtt atccagagct gaaattattt tccacagcaa tagctgttcc
    51001 tgtagatgga gatatcagtt tcagcattga aagcagtgaa tctcaaacgc taaagaatgt
    51061 gaatatatat cctgcggcaa ctatgttgct ggaagaaagt gaaccaatat ttgttaataa
    51121 gccaaactat aaagcatatt ctaatcgtga gctgtatcca cttcaagtag ttcaaaaagg
    51181 tgaacctgct tttgttggta accgtagatt tgttccctta tttatttgtc catttcagta
    51241 tagagctcaa agtaaagaac tcattatcca cagaagtatt actgtaagcg tatatgtagc
    51301 aggttcaaaa aatgcctcca agaattggca actgaatcct aatcctcttg ataatgcaac
    51361 tccatcttta ttcttgaatg aaaacagctc caaaatatgg cgcttggaaa aaacccgcga
    51421 tacaaattat acttcaccca aaaatggcac ttccggaata aatgaaattc aaataattgt
    51481 tgataaagaa ggtatttata aagttgatta ccattaccta atggactata ttaatatgat
    51541 ggttgattcc ttacaggtaa atatgaattg gactcctgcc aatgtagacc ctcgctatct
    51601 ggaattgagt gatgaatatg gtcaagtacc tattaatttt gttggtgaaa atgatggtat
    51661 tttcaatccg aatgattatt ttgaatttta tggtgatatg cataaagggg atacttccta
    51721 tatggatgat tacacttctg aaaatgtata tactttgaaa ttggtggatc gctttggagc
    51781 aagaatggct gtagaaaacg gtgggttgat agaatccaat atctcaaatt gtattgttcc
    51841 agatgcttat gaagatactg ttcacttcga ggaacaactg ataagcgata aattgggtca
    51901 gggctggagc tcaatagacc agaattacta ccgagaagat gtttggttct ggagaaaaat
    51961 agatgctccc gacttggaaa ttgtacctat agaattgcaa tatcctaaag atactacaac
    52021 tcgctatgcc tctgctaaaa tatgtttgat ggggcttact tattccgaaa cattaaccag
    52081 tggacaattt gaccacgagg catctattcg tctgaatcaa gcaatgatta atacccttac
    52141 ctggattgga cagactgaaa agattttcca aaatcaaacc gaaatctcca atactgcttt
    52201 aagacatgga gtaaataatt tatatatagc tctatcaggt aatactgtta tgggcgatga
    52261 ggaacaagtt ttattggatt gggcagaaat aaaatattgg cgggaatata aaacagatca
    52321 ggattatata aaatttacca aaccttctaa ccttcctgat ggtttatatc agtttgaagt
    52381 gggtggattc agtaatgccg atatatccgt gtacaaaatt ggttccagca tattcaattc
    52441 tgtgcagata gagcccttta atattaatgg agatgctccc tggacagtta ctttgcaaga
    52501 tagtgttgct tccgatgccg taagatattt tgctgtaacg gaaaataaga aaaaaacacc
    52561 gaaaaacatt aggttgaatt ttcccagtga tttgaaaaat ccctatctta gtgctgatgt
    52621 cgttgttatt actccccgga agtttactga tgtggaaggg actctgcaat taaaatcact
    52681 gtgggaatcg gaagggcaca ctgtccaaat tgtggatgtg caagatatct atgacgaatt
    52741 taatttcgga atcactggta gtgaaccaat cagagatttt attcgttatg cttataacaa
    52801 ttggagcagt ccacaaataa accaggttat tcttttagga gaaggtgtag acgatacacg
    52861 agataacagt tattctcgaa tgtataatct gataccagtt aaaaagacct ggacttataa
    52921 tcacggagct acagcaagtg atcaatggta tggttgtatt gtcggaactg ataatattcc
    52981 cgatgtatcc attgccagaa ttagcgtttg gaaggaagaa caaattttgg cttatgccgc
    53041 taaggcaatt tcctatcggg aaaatctaca aacctctcgt ctatggagta gtcatttaac
    53101 cttcacctcc ggagggaaaa ttaccgatgg aaatgatctt tttgctcagc aatcggaacg
    53161 cattatccgg aaaacaattc ctcctgaata tagagtaacc agagtttata cttccacgca
    53221 aacagttagc tcggattact ttggcggaac ctttaacttg aaagacgcga tcaattccgg
    53281 aactcagtat gttcaattta tgggtcatgg aggaggaaga atttgggcgg actataatct
    53341 cttcaatttt aatgatgtag caacgctcaa caatcaagtt taccctgtag ttttatctct
    53401 tgcctgttat tgttcagctt ttgatacaaa cgggatggct tctatcagtg aagcattagt
    53461 tttgcagcct ggtaaaggag ctattggaac tgctggtttt acgggtttgg gttttaggga
    53521 tcatgatgag gactggggtt tagcatattg cgaagcacta tttaaacaca atttcgataa
    53581 tattggtttg gcaaatatct atgctttagc caggttctat tccacaactt attctcctgc
    53641 agccagatat gctttaatta acggttttgc ttatatgggt gatccgctta tcaaattacg
    53701 caaaccaata aaggatattc ccgtctcggc taatagctat gttctaaatc ctggggatac
    53761 tctttatgtg aatgcagatt tcccttcagg gattaatgct gcgcgtttat ttataatgaa
    53821 accaagcgga aaaatagtta atgttcctta tgatttacca gtttatccgg atggccattt
    53881 tacagcgtct tatgttaatc cagaccaaac taccgataat tatttccgca aaattatgat
    53941 agctggttat tctcctagtg atgaatttgt tggcatttct cagtatagtg tgggcagacc
    54001 gaatattgta catcatagtt ttattcctgc agaacctgcc tggtcggatt ctgttttatt
    54061 tactgctaaa gtatttagtc ccttgccagt tcagaatatc tattgtaaag taagaaccga
    54121 tagcacttcg caagtaatta attgggtaga tttgccgatg caacgatcgg aaacggatag
    54181 cactttatat gttacggcta cgcgtttagg aaggcaacct actcgcaaag aaatattcta
    54241 taaatatgtg ttggagaccg aagcaggtgt ttcggagacc attttgcaaa gttatgtagt
    54301 ggcaggacct gatttacttt taaaagacat aaaattggaa caggatggaa ataacttagt
    54361 attaaaagtg ctgggaacca atgttggaaa cactccttca attactacag atttaaaatt
    54421 atatgtgggg actacggcaa ataaccttgc ttttttttct agccaaagtt atcctccttt
    54481 ggaagtaaat gaattgcgtt gggacagtat ttctcttgct aatttaccca atgccaattt
    54541 atttttggaa gtaagagtaa ataccagcaa cacttttccg gagtgggaag catatgttaa
    54601 tattggcaac tatatccgtt tgcaggttcc tctgaattat tatttgattg atagttcggg
    54661 ttctactctt agtagcatag ataataatct tcaatgcaca atacctgaag ggcttgttcc
    54721 cactaatgaa caaattcttt ttgccgtaaa tactttatca gcattacctc ccctcaatca
    54781 acctgatgta caaaatatta tgatgaatgc tccagatgga tttagtggga ataaatattc
    54841 tattccttac gagattttag cattaggcac aggtgttaca gattccttag gggttctaaa
    54901 tggaggtaag aaactattcc tttcatttaa ctatagcgca attgattctt taactcaggc
    54961 acaggaaaat gctaataact tcaggattta tcgttataat tcccaagtta aaaaatggat
    55021 actaatcggc ggttttgtag atgtaaatct aaacaaagtt caatttgagg ttgatcgaac
    55081 cggaatttac agtatcttca gaaatatcga tagttcacca ccaagtatag atgttaatgt
    55141 tcaagaccag gaatttactg tcggtggcta tgttgctggc aacgggatta tttcgctatt
    55201 actatctgat gccaacggaa ttgatgttat agataattcc attcaacttt tcctaaatgg
    55261 aattcctatt cctgaagaag attatgttgt ttccatcaat ttggaaaata tcaatagaat
    55321 tccgatcaaa tatcagctat ctttaggacg cgggatccac gaactaaaag ttaaatgtcg
    55381 tgatttaaat ggaatgcttg caacccgcga tatccaattt accgtgaatg acaaatttga
    55441 tgttatcaac cttgccaatt atccaaatcc tgtgttagga acaggaggcc aaggcgcagc
    55501 tattgatcct aaaaatgaag gacgcactcg ttttacttat atattaactg atggagctga
    55561 tgaagttact atcaaagtat atactataag tggacgtttg gttaaaacct ttagaaattt
    55621 acctgtgggg gttggttatc atgaatatcc tcgtacagtg tatggttggg attgtaaaga
    55681 tgatagcggt tacactttgg ctaatggtgt ttatttttat cggataattg ctaagaaagg
    55741 tcataaaacg attgaaaaga cacaaaaaat ggcaattctg aattaaggag cttaaagtga
    55801 aaaagataag tatcctgttc tcgcttaccc tgatgtgtct aacgccactt tttgcaggac
    55861 gttatgcagg agattttatg atgattggtt ccggagtgcg tcctttggga atggggggtg
    55921 cttttagttc attggcagat gatggttctg ccatatattg gaatccggct ggtttatgtt
    55981 ttattaccga ttcagaggtt tcggctatgc attgttttct ctataataat cttgcttctt
    56041 acgactatgt tacttatgct cagcccctac ccaatgatgt aactatttgc ttaaatgtaa
    56101 ctcgcttaac ggttagtgac attcctcatt tcgatgaacg ctatttgatt ggaaccaatg
    56161 tagatcaacg gatcaataat agcgtatatc atttgccggg tgttccagac agcaaatttt
    56221 ccagttatga tgatctttat caatttgctt tctctaaaaa aattcattat gatgctaata
    56281 tgggttggga attttttgcg gttccttttg attttggttt cggaggcact gttaagctta
    56341 ttaaaaggtc tatttgggat aggactggaa ccggaactgg tatagatatt ggttttaaag
    56401 caaaaacaga ccttgctgtc atttttgatt gggaagatct tggtaattta gattttggca
    56461 tcaattttca agatattgga ggaactgata tcacttggga tacaccaagt aagataagag
    56521 atgaagtttt atacaataca aaaaccggag tagcgattga gcaacccata ccaaaactga
    56581 aatctattgt aactttagcc tatgattatg attatgtgta tgggggaaca aaccactatg
    56641 gtgtagattg ggaatatgat aaaaagtgta atttgcgcct tggatattac gacactaatt
    56701 ttagtgcagg tgcaactatc aaactttatg gagttttgct cgattatgcc ttaattacta
    56761 atccaatagg tattagcaat cgtttgggct tacgagttag attctaagga gttctaaaat
    56821 gagaaagatt tgcatattag ccggcctttt gttacttgtc ttaacctatt cctgtgcggc
    56881 agacgattct ccagaaaagg ataaaattcc tcctgcagct cctgacttat tagaacattt
    56941 gggtgatacg ggagatgatc cgattactat agacggaatt gaagtaatct tagatgatga
    57001 taataatggt attgatgctg ttccagatgg caaatggata aaagttcctt ggaaaaaatt
    57061 tgtagataac gacttaagtc atgtaaaggt atatcgctat actgtacaga atcctgaacc
    57121 attgttgatt gatactgttc ctgcttcaga ggattattat ttagaccgga gttttttgat
    57181 agaaagagaa tggtattatt attatattga actttatgat gccagtggaa atttcagcgt
    57241 ttccgatact gtttcctatg ctttattggc aaaaagtact ctcacttctc ctgagggagg
    57301 ggcagctgtg aatcctaata atttaagact ctactggaga attggtgata gccagacaac
    57361 tgaattcagg attctgattt gggacgagca tactggagaa ctggtttatc atacagatta
    57421 ttcttatact cctaatgacg aaaatcctgc cccacccaat gttccctttc ctgtgatgac
    57481 tcctgctcct gtaaatggac aggtttttcg ttggagagta gattcctttg attatgattc
    57541 ggaacacgaa atgaaaatgg gttcagaatc tgaagaaaga acttttataa ttcaatataa
    57601 atgaatatag tgctttagaa tagtgattat gcttatgaga aataacttga ctttaacagc
    57661 taaataaaga atgtaacgca aaatagaaaa gaggatattg tgaagaaaac attgttggtg
    57721 tttctacttg ctggttttgc ttttatgtta ttcggccagc agacaattaa ttttacccca
    57781 tcctccaata tttctgccta tacctatgaa ggtattcgca gcggagtgga aaagctgaag
    57841 atgaataatt atattttggg ccaggtttat aaaccgggtt tatatactgt ccctgatgat
    57901 actgattttt taactttgct ttctttagca ggagggccaa gagaggatgc taaactatct
    57961 aagataaggg tcgttcgtcc ttcttctgaa ggcgataaag ttatctgggt taattttaaa
    58021 aaatatctgg aaactggaga tgctacttta attccgcaat taaaaccggg tgacactata
    58081 attgtctcag gaactatttt ttatgctgtc tctcgagtgg caagttttct ctccgactta
    58141 accgtagctc taagtatgta tagtataata actggatttt aggaggggga ttcaatgctg
    58201 gaaaatgaaa ataaccaagt tcccgtacaa gaagaaataa aactatctga ctactttaga
    58261 ataatcctgc aatatcgtta tctggtaatt ctggttttct ttctggttat tgttgcgaca
    58321 gttttatata ctgctcgttt accaaaagta tattctgctt cttccagaat tttactggaa
    58381 gatgccaata aacaatctga tcttatgttt ctatctgctg gcggattagg taaaaatact
    58441 gtaaataatc aaattgaact gattcgtagt aaacctatta tgaagcttgc ctgggaaatt
    58501 atgaagaaat atcctgattg ggatactttc cctgcaagtc aactacctga tcctgttgcg
    58561 agtttaaata aaatgaaagt agaatcaaaa cgggaaacag atgttttaac tattagcttt
    58621 gaatctacaa atccagctga agcaatggcc gcagtgaatt ccatcgctga agctatccaa
    58681 caacaaaaca cccaatatgc tcgtttggaa tttactacta tccgtgaatt tctggaaact
    58741 caactggatg ctatttctcg ccgattgcaa agttctgaaa atgatttaag agaattcaaa
    58801 aatttgaacc agcttacgca attaaccgaa gaaacaacta aactaatcga aaaagcatcg
    58861 gatgtagaag cagaatatga agcagcatta acagagcaag cagtgaaggc taaaaccctc
    58921 gatatgctta atcgtcaatt acgcgaacag gattccctgt tagttaatat agataatatc
    58981 cttaaaaccc cctatataaa tgaactgcgc aaacagattg ttgatactca atcccttatt
    59041 actaaactcg ttacaaaaaa cgaatatcct cttgaccatc ctcaaattca gctactttat
    59101 cgcgaaatcg aaaatgccaa agaaacactg gatcgcgaaa tccgcaagct ggtaagtttg
    59161 tcgataaatg aagatcctct taaaacccgc agcgacctat taaccagaat tattcaagcc
    59221 aatgttgatt tggaaatggc cagagccaaa gttgatggct tggaacaaac aaaagagatg
    59281 tataatcaac gccttattgc tattcccaac acggaattgg aattagcaag attaacgcgc
    59341 aatctgatgt tggatgaaaa aattcatagt atgatgatgg aaaaatatga ggatgctaaa
    59401 attgcagaac aggccaaaat tggtaatatc cgtataatcg actatgcaga acgtcccaca
    59461 attcctatta aaccacgcgt ttcgatgaat attctcgtag gtattatatt gggtttgggt
    59521 ttaggaattg gatctgcttt tttagtgcat tctttggata caaaattacg cacgctggaa
    59581 gatatggaaa gctttgtccg gttaccaatt gctggaacga tacccgtaat tcgcgaagcg
    59641 gaatctaaac ttcaggaatt taacgatatg attaattctg ctacaggaga aaatcgagaa
    59701 cagcttactc gttcgatgca ctatgtaatg atgcaattgg tttctcatta tgccccaaaa
    59761 tctcctattg cagaagcata tagaacttta agaaccaata tcttagctaa aaaaggtgaa
    59821 ggaagtgttg caattcttgt tacttcttcc ggacctaaag aaggtaaatc tacttccata
    59881 gccaatcttg ctataactct tgctcaaatg aatagcaagg ttattttagt tgatatggac
    59941 ttgcgtaggc ctactgttca cactaagtta ggtctggaaa aagaaaacgg caccagtgat
    60001 tttcttatcg atccggaagt aagtgttgag caagttatca aaccctctgg aatagccaat
    60061 ctggatgtaa ttaccagtgg gtttattcct cccaatccat cagaattaat tgcttcaccc
    60121 agaaccgatt ttttgattga agaattaaaa acacgctacg actttgtcct tttcgattct
    60181 ccacctttaa ttgctgttac ggatgctctt attttaacta agaaagtaga tatgacctat
    60241 cttgttgtcc gttgtggctt taccgataaa ggtatcatca gacgcaccaa ggaacttatg
    60301 gaaaatattg atggtagaat agatggtatt atcgtgaatg gtatttatgt ccaaaaatat
    60361 tacagcaaac agaattacta ctactattat tactactact attattatta tggtgacgag
    60421 gtacctcaaa aacaaaaaaa gaatgttaac cgcttcctgc gcaaaaataa atctgtttct
    60481 tgaattaaca ggtaagcttc ctaataatta tcatcaggtc gatactgttc tttgcagtat
    60541 tgacctgttt gattatctgg actatgaagt tattgattct ccgcaaatag tcctttcttg
    60601 caatattaat cagttagcaa atgaaaataa cctcatatac aaggtagcat cctatttgca
    60661 gaatgaatat aaaatcccca atggaatatc cattaagcta aaaaagtcga ttccaatagc
    60721 agcaggttta ggaggtggta gtagcaatgc tgccaattgt atattagcat taaataaact
    60781 ctggcagcta catttatcaa aagcagaaat gcataaaata gctgctcaat ttggaagtga
    60841 tatcaatttt tttctggaag ggggaacagc aagaggggaa aatagaggag agattataac
    60901 aaaaatgccg gatatcattt tggataacat actattaatt aatccaggca tagcaatttc
    60961 cagtgcagaa gcatataaac tggcttatct aacttccaaa caagaacaac attattttga
    61021 tattaaaaat gtgtatagct cctgtttcaa tcgcttggaa gctggaatca gaaaagctta
    61081 tcctattgtg gatgaagtaa ttaacacgct taaacaatat gaagcaaaag tgtcaatgct
    61141 tagtggaagc ggatcaactt gtttcggaat ctttgaaaac atcgctcagc ttgaaaagtg
    61201 caaggattat ttttctgaaa aagggttctg gacatatcga gctaaaacca ttaatagtga
    61261 aaatagcata tcctaattag gcttttgtgt gcaaagataa gtagagataa gtaaaataga
    61321 ttagctgata aatgatggga tatctgccca tagaaaatgg gtaaaattac tttgttgtga
    61381 tggaactttt attatcattt ttatgttgct ttctccttta ctaacatcat caccctataa
    61441 tcccaaaaca aatatttaac gattgcctaa tctcgattga ggatcgttta aaaaatgtta
    61501 gagtgtcctt agaaaactta ctaggcaagt agtgcagatt ccttgtaagt agtttataca
    61561 ctatatagtt atcttactgt aaaatagata agataaccag aaataagaag gataatcttt
    61621 ttcattctgg cgaggacatc agttctgtga aatggggagt aaaggaaaaa agtacccccc
    61681 tttaattatt tctgttacta aggaagaagt aatatatcat ttctacattc accgggaatt
    61741 tcactccatt aaaaaaatcc gaatatagaa gccacatagt ttatggatat atttagtact
    61801 aaggtgatga attaaaatca tctaaaaaaa agctaagcac aaagtctgtt aatatccttc
    61861 agtttatttg ctaagcaaga actggtgaac tcattttcct caaaccttaa agtttgggat
    61921 tgatattgaa tacaatccta atatattgat aggtcatcag gcatttacta ctttagtata
    61981 aactgcatat tgtattcaag cttcgcactt cgcccttaaa ggaaagcgtt aatcaggtgt
    62041 taatctaacc ttaatcaagc gttaattatt aacgcttgat taaggcttga taaacgatta
    62101 atcaatggct tgcagtaagg aaaattggag acaacaatat taggatttta ttataaaagt
    62161 gattgtttaa gttagtatag tacactttta agagggaaat aatcgcaata tctttaagtt
    62221 gttactttag gacttggaat tgtaattgcc acttatatgt atgttatatg aaaaaacaaa
    62281 aggacgatac cttttactcc gaccacccag atcggttttg ttaaatagat tgacgatttt
    62341 agggctttgc agaaaataac gctatggata atataactcg taaaacaaca cgccgcataa
    62401 tgttaggtga tattccgatt ggaggtaatg cacctattag tattcaatct atgttatcta
    62461 ttaagacttc aaacattcct gaagcaacaa cccaaattcg cgaactgatt tctgcaggat
    62521 gtgagataat ccgctgtgcg gtaatggata tcagtgatgc taatgcgatt aaagaactaa
    62581 agaaaatatc caaagctcct ctggttgctg atattcattt tgactatcgc ttagcaatag
    62641 cttccattga aaatggaatt gatggtctgc gtatcaatcc tggcaatata ggaaaaattg
    62701 aaggtgtaca gaaattggtt gcagcagcta aagaaagaaa tatcccaata cgcatcggag
    62761 taaatagtgg ttctttgccc aaggatttag tgcaaaaata ttctgttagt gcagaagcaa
    62821 tggtagaatc ggcactaagg catataaaaa tattagaaga tcttgctttt tatgatatta
    62881 aagtttctgc taaggcatca tcaatacctt tgatgttaga aagttataga aagttaagca
    62941 aggcttgtga ttatccttta catcttggaa taacggaagc tggaactact ttaagtggct
    63001 ccattaaaag cgctatcgct ttgggtatat tattagaaga aggaattggc gatactataa
    63061 gggtttcttt aactgccaat cctgttcagg aagtgatagt agctaaggaa attttacgaa
    63121 accttggctt aagaaaggga ttaaatataa tttcctgtcc tacctgtgga cgcaccagaa
    63181 tcgatctaat tggattaaca gaacaagtgg aaaaagctct gaagaactat agtgattacc
    63241 ctttaaccat tgctgtaatg ggttgtgccg taaatggtcc cggagaagct aaagaggctg
    63301 attttggcat tgctggaggg attaaagaag gtttgctttt tgcaaaaggt gaaattgtga
    63361 aaaaagttcc ggaagaacta ttagttaagg aactgcttgc tcttattcaa aatcatatag
    63421 gtaaacaata aaagtgcttt taaaagtctt ttttactttc tttaaaattg gtgcttttac
    63481 cattggtggt gcttatgcaa tgattccttt aatcaaaaga gaagtctgcc aaaaaaataa
    63541 atggcttagc gaagatgaat ttttggatgg tttagcagct gcccaaagtt gtcctggtcc
    63601 cattgctata aacataagca tctatgtagg ttatcatctt gcgaagggta aaggtatggc
    63661 agttgctgta ataggaacaa ttttaccttc ctttataact attctgataa ttgctatgct
    63721 ctttcaacaa ttttccgaaa tagtaattgt gcgcaaggca tttcattctt taagagctgc
    63781 cgttgtagct ttaatagctg ttcccctttt acaaatgagt cggcaggcag gtttaaagct
    63841 atccaatttc tggtttcctt tagctattgc tattctggtt agtttttttg cagtaagccc
    63901 aatttactta attataataa ccattgcttt tagcatctac caaagcatca gaaaacaaaa
    63961 gatatgattt acttgaaatt gttctggacc ttcttcaaaa ttggactctt cagttttggt
    64021 ggaggttatg caattttagc aatgatccgt caggaagtag ttttaaaaaa cgcctggata
    64081 tcatcttcac aatttatgga tgtagtagct atttctcaaa tgactccagg cccgatagct
    64141 ataaatgctg ccacttttat cggttaccaa aaagggggat tatggggagc agttcttagc
    64201 actctgggag tgatttttcc ctctttgatc attatgacct taattacgat aacctatata
    64261 aagctgaaaa atcaaccctg gttcaaaaac atcttccaaa aattgcgttt actttctttg
    64321 ggattaattg ctgctgctct aattatggtt ttgggagatg cttttagaga cctgttttct
    64381 atcctggttt ttgctggctc ttttttggtg agctggagat ttaagctgaa tcctttttat
    64441 ctgctaatgg gagctgctgt tataggggtc atttttggct aatttgtaaa cgccaaaaac
    64501 acatttgtaa tttccttggt agactatttt actccccaaa tatctttggc ataagctaag
    64561 atagtattat cggaggaaaa tttgcccata ttagcgatat tagctaagac cattctattc
    64621 catttttgtt tatctttata ggtttctgtg gcttctttat taacccgaat aaaatcctgt
    64681 aaatcagcta ttagacaata actatctccc tgatataaaa gcaaatctgt caatggagta
    64741 aataaacctg gatttaaggg attgagttca tcggattcaa taaaatcaaa tatccgcttt
    64801 atatcttcat tttgacgcat catttcgtaa ggatgataac cctgttcttt caaataatga
    64861 acttcggcag ccgtcattcc aaataggaag aaattatctt cacccactgc ttctcgaatt
    64921 tctatattag ctccatctaa agttccgatt gtaagtgctc catttagagc aaatttcata
    64981 tttcccgttc cagatgcttc tgttccagcg agggaaattt gacgggaaat ttcagcagcg
    65041 gggataattc tttctgccaa agttactcga taattgggca gaaaacatac atttagataa
    65101 gaagacagct gtttatcttt ttcgatgtaa tcacccaaag cgcaaataaa acgaataatc
    65161 aattttgctg taaagtaacc tggtgctgcc tttcctgcaa aaaagaaact ttgtggagtt
    65221 aactctttgc catcccggat ttgatttatc aagtagataa ttcccaaagc attaagcagc
    65281 tgtcttttat attcatgaat gcgttttgca tgaaaatcaa aaatagtatc aggattaaac
    65341 ctacgatcgt gcatatcttc gtaatattta caaaaatcca gcttattttg atatttcact
    65401 tctgccaggt cttccagaaa agagctgtca tttttatatt tattcaaatc tgaaagctgc
    65461 tctaaatcag ttttccattc ggtaccaata gctttagtaa ttaaatgaca taaaagtgga
    65521 ttacataaca tcagccaccg gcgaggagtt atgccattgg ttttattatt gaatttatct
    65581 ggcataagag aataaaaatc gctgaatacc tgatgcttta acagttctgt atgcaattcc
    65641 gaaactccat taatactatg ggttcctaca atagccaaat ttgccattcg cacacttttt
    65701 actggttctt cttctatcaa agagaccctg cggaggaagt catcatctac ttttctgctt
    65761 atttgtatat cagagagaaa acggtgattt atttcataga tgatttgcag atgacgaggc
    65821 aacatttttt caaacagctg aacttgccat ttttctaacg cttccggcaa aattgtatga
    65881 tttgtatacg aaaggcactt ttgggtaata tcccaagcgg tttcccaagg tatatttttt
    65941 tcatccacta aaatacgcat aagttcagga acggcaatac agggatgagt atcattaagt
    66001 tgaattgcag ctttatcagg aagattgcgt agatctttat tctttttaaa aaacctgcgt
    66061 aagatatctt gtaaagtagc acacacacaa aaatactctt gcttcaatct aagttctttc
    66121 ccttgcattt tgttatcatt gggatacagt accttggaaa tcatttctcc attattcttt
    66181 tcggctacag cttgtacata atccccttca ttaaaatatc tcagatcaaa ttcacggctg
    66241 cttttggcac tccataatct taaagtattt acatagctat tttgaaaacc agggatcaaa
    66301 taatcataag ccatagccat aaccgtcact tctggttgcc aatgtttctt tttggtgcca
    66361 tcctcattga taatttctct aacagtccct ccaaaattaa caggaaataa tcgtgtcgga
    66421 cgaggaattt cccaaacgga accatatctt aaccagttat caggtgtttc tacttgttca
    66481 ccattgataa tgtgttgaaa gaaaatacca tactcataac gaataccata accgtaagca
    66541 ggtatttgta gggaagccat agaatctaaa aaacaagctg ctaaacgtcc taaacctcca
    66601 tttcccaatc cggcatccca ttctagttca cttagcattg ataaatcaaa accaatttca
    66661 gtaagagctt taatgctttg ttgttggata tccagattga gtatggaatt ggttaaagaa
    66721 cgaccgatta aatactccag ggatagataa tagactcttt tcctttgtaa agcatattct
    66781 tgaggttgag ttatcagcca gcggtctaat aaaatatctt ttacggctga agcaaaactg
    66841 atataacaat cataaggggc agcagaaaat tggtccttac caagatcgaa ttttaaatgt
    66901 tccataaaac gaagcactat ttcttctact gtaccattaa tgctaagctg agaaagttta
    66961 gtgcaaaaat catcgttcat catatctcct cccgctaatc ataagtccat tataaataat
    67021 aaacggtttt caggataaac caaataagat gtttaatata gaaaagaagt gtaactgcca
    67081 taataattaa tgtcttccaa cttcttcatt tctatttatt ccctgctaaa atataaattg
    67141 atttggaata attcttgtat gtattatatt atctataaaa gtctaggagg atatatgaaa
    67201 ttgatgagta actatcaacc aaaactaatg gttatcattg cggtttttta cctgttttgc
    67261 tttggtataa gggctgctgt ggttcagcca gaaattgctt tgctggctgc caagaattat
    67321 cttcaggaaa cgggattatc caaatccgtt aatactgatg actatcaaat ttttacttat
    67381 aatagcgcag ggaaacttgc ttctctgcca catatatatg tacagaatga gaatccggtt
    67441 ctctattata tccaatatgc cgatggtaat tttgctgttg tatcagcaga tgataatttc
    67501 tatcctgtct tggcttattc taatgaaggt cttaccagga tagataattt accttcagcc
    67561 ttttattatt ggctggatac atattctgct caaatacaat tgattagaga aaaaaatgct
    67621 ctatatccgg aaaactctca gctttggcag agtttactgg aaggaactta tactaattta
    67681 agcagaactg atagatccgt aggtccttta cttactacta attgggatca aagttggcca
    67741 tataatgctc tatgtcctgc agatcaacac ggtcctggtg gtcatgttta tgctggttgt
    67801 gtagctactg ctatggggat ggttatgaaa tattgggatc atcctgtttc cggagttggt
    67861 aacgaaagtt actattgtcc tggctatggt tatcagagtg ctaattttga aactaccaca
    67921 tatctttggg atcagatgcc caattccata agttctaatt acttacctgt agctacttta
    67981 ttatatcatt gtggggttgc agttcatatg ggatattctg cagatggttc aggagctcaa
    68041 agtacagatg cagctgaagc acttgtcgat cattttcgtt atcctacagc tcaatatgaa
    68101 agtaaagata gctattctaa ttccgaatgg aacaatctaa tgacagctca aattgataac
    68161 gggactccgg tatattatag tggttatagc aatgagggtg ggcatgcatt tgtcctggat
    68221 ggttatgata cagcaaatca ttttcatttc aatttcggct ggagtggatc aggaaacggt
    68281 tatttttaca caacggatgt taatggattt tctacctggc agggagccat tataaatact
    68341 attccggaga actataatat caatactatt ccagtagtgc ttaatgccaa caatgctgaa
    68401 gctggtgata attttacctt ggaaataaag acgaaacctg tattaggtaa ctggaatgta
    68461 aatcactatg atattatgct ttactatgat aatgaaaatt tagattacac tggctacagt
    68521 gtgcagggaa ctttatctca ggaaggtaat ataaccatag tggaaaactc cccgggaaat
    68581 ctaagtattg actggaatag caacactagt ttatgcggtg ggggagtgat tattaaactg
    68641 aatttcgttg ccagagatgc aggcgaattt ctttttgata ttttgagtat gcactataat
    68701 accctacctg taaacaatgt tgtttttact atggtaaata ttactgctcc tgtggctacc
    68761 ttagcagaaa gtaatattac tctttcaaac attatgcatc ttgtttacaa ttcaattgga
    68821 tttacacaag taaatacttc ttatctgctt ccttcctgga atgttaatca ttaccaattt
    68881 aaccttaatt acaatcctgc caagcttgaa tatgcaggat acgatgtaaa cggaacatta
    68941 agtgatggct gcgaaatcaa tgtagattct tctactccag gagttataaa tatatcctgc
    69001 gatacggaaa gtaaattcgt tggctcaggt atattaatta agccccaatt tagagctatt
    69061 ggcaatacta gtactatttc cattactcaa atatctctta gtgattttta ttataacgac
    69121 acagcagttt taggttctgg gagtgcaaat gttgttcttt caccttatac tgctattgac
    69181 gatgaagtga tttcaactcc aaaacccaaa ctggaaatat atcctaatcc tttttccgga
    69241 aatactacca taaaatttac tggaacagcc aagggagcgg ttacaataaa tgtatataac
    69301 ctgaagggac aattcgtgaa ggaattaact attagcgatt tttcccaaag ggaaattcaa
    69361 tggaatgcca cagacagcaa aggcagaaat gtttcggaag gaatttactt tatgcaatgg
    69421 cagcaaggta atttacgagg cacagataaa gtattaatca tcaagtaaat aatataaagc
    69481 ttgggaagat tgaagttgtt actgctgata acagcttttc tcttcccttt cattttttat
    69541 aatgtaagat tcggaaaagc ataagcagct ttaagattaa ttcgatattc ttgtaatatg
    69601 taatggagga tatttctgag taattaggat ttccttcaag ttatatatct tatttctgca
    69661 ctttagttat ttatgaaatt ctgtttcctt ttcttctagg aaacttcgaa ttgtttcttc
    69721 tactattgca atatcctctt tattgtttac ccatatagga tcggaataaa tcattgttaa
    69781 tttagcaaag ggctttggga agcggaattt atcccaggaa ttaaacaacc attctttatt
    69841 ggcatttacg gcaactgcta caatgggaat tttagccagg taagcaatct gaaaaagtcc
    69901 gggatgaaaa gtgtagcaag gacctttagg tccatcgggt gtaatagcta aggaaattgt
    69961 tttagacatt ctaatcatct ctcgcatagc cattgatcct tttctcgagc tggaaccgcg
    70021 aactggaata taaccaagtt cggataatgg cccagcaatc atttctccat ccttggaaga
    70081 agaaaccata acagctgctc cttgatcaat tcgttgcaga gtcatcaaaa gcaggtttct
    70141 atgccagaac atatagatac agcgtatcct gtctgatgtt tcttgattaa ttacttcgaa
    70201 ttttaaggtt ttacgcagca gccgcaggag cagagcaccc aattttcgtt ccaggtaata
    70261 gaatatttta accataggta tctaatagat gacggactat ttttggcatt tccacagaag
    70321 gatgtttatc gctcaataaa gctttaagtt tatacagttg tttacggatt tcctcatttt
    70381 tttctggatc gttcagtatt tgcattccta tcttatatat attagagggg ttcatctctt
    70441 tttgaatcaa ttccggtagg atatcgttat ctaaaattat attaggtaaa ccgatccgtt
    70501 ttatttttac taaatgtctt cctatcagat aagaggtaaa attagcttta taacagatta
    70561 ataggggggt tccaataaat gcagcctcta aggttactgt tccggaagtg cagatcaaaa
    70621 gatcgcaata tttcatcatt tcataggtat agccatcaat tagtgtgata tgttttttac
    70681 cttctatata gctcatatat aattgatgat ttacactatg ggatttagaa aaaagcagtt
    70741 catattcatt cggatcccac ttttcacttg cctgcaaaaa agtaggtaac atttttgcta
    70801 tttcattatt cctacttcct gggaaaaatc caagccattt tttatttggg tcaataccaa
    70861 aaaattgagc gaaggcatca cggctaagtt caatattaac ttcttccgca atcgggtggc
    70921 caacataact gcaattaata ttatgaatag atagcttttc ttcttcaaaa ggcaaaatac
    70981 aggcaacata gcggacagaa tctcttaatt gatataccct tttatgtttc catgcccaaa
    71041 attgagggca gataaaatat aaaacaggaa tccgtaattc atcagccatt ttagcaactc
    71101 ttaaatttaa gccagggtaa tcaaccaaaa tagcaagatc aatttcttca gtagcaaaaa
    71161 gtttgcttag tttatgttcc actttcaaga aaaaaaacag atgctttact acttccacaa
    71221 aacccataac cgcaaagcgt tcaaagggaa agagaggttt caaaccttgc ctttgcatcc
    71281 ggggtccacc aattcctata tgcgtaagat ttccgaattt atcattcaga gctttcatca
    71341 ctaattcgga atgtagatca gcagaactct cacccaccag ccaaaagatt ttataacttt
    71401 cttttcttgt cataccgcca aagttcccat aagcgataaa atagttacaa tgatagctgc
    71461 aagaattact cctaataaaa tacagaacat tgcacgccag aatctaatac caaaaatatt
    71521 agcagctaag gcacctgtcc atcctcctgt tccaggaaga ggtataccaa caaaaatagt
    71581 taaacctatt gcttcatagt tttctattga tttgcctttg cgtctggttc tggcaaaaat
    71641 ccaatcagca aatttcttac ctggtttcac cttacttatc aaagaaaaaa accaatctac
    71701 caataacaaa ataaatggaa tgggaatcat attaccgatt atggataaaa gcgctgcctt
    71761 aagccaatga aaattaaaca agaaaatagc tacaggtata ctacctcgca attcaataac
    71821 cggaatcata gatattataa gtactatcaa ttctggtgaa acattcttag attccaacca
    71881 ggcaactgtt ttatctgctg tagaattacc aaaaagcggt tgtgacaaaa ctactatcaa
    71941 gatcatcaac agcaacggtt tgaggtattt attcaagttc actccttagc atctttgatc
    72001 tttattccat ttattattag cctatttttt gtcaatttga tttgtaacag cgtttgagta
    72061 ataaaagtaa aaagtactct ctcttattat cctttccaat catctgcagc tattatcatt
    72121 cgagggcata atatcatagt ccgaatgaaa cgatgttatt atatgtgagt tgtagctgaa
    72181 taagatatta ttgttaatcc tctaaactaa aaaaaatgtt ggcaaacgaa gatgtataaa
    72241 aagcacttat gttactttta tgagaattca gaataggcag aaagaccatt gtggtggagc
    72301 atttagaaat ttcatcaaaa acgatagatt tgagcgacag ggtttaaccc ggctccaata
    72361 ttatcaagat aatatccttg cgtgtaactt gttcaatggt tttattttac actgagaacc
    72421 ttgtatataa actatattct gcataatcag aaaaatctgc aggtgtatat tttctcaggt
    72481 acaccaagtt cttgggtaaa tagttagcca aaaggaatca gaataatctg cttatcacaa
    72541 tttcaattga caaaatattg gtatagtata tttgttacgc aaagtgactt tactggagat
    72601 atattatgaa tgaccagtta caagaactac tcaaaaaagt ttatgaagaa ggcgttgcca
    72661 aagccaatgc tgaagctgag aagataatca attctgctaa agaacaagct gaagacactg
    72721 tttcccaagc caaacagaaa gcggaaacaa ttttaaaaga ggcagaaaaa aaggcagcag
    72781 accttaaaaa gaacacagaa tcagatttga atatggcata taatcacact ttaactgctc
    72841 tcaaacagaa aattaccgat cttattcttt ccgaagctct caacacaaaa ctaaatgctg
    72901 cattcaatga aacagaattt gttaagaagc tcatttttga aacacttact gcctggaagg
    72961 aaaatgcctc ctctggaacg attatgatca gcgagaaaat gaaaccttta ttagatgatt
    73021 tttatcttaa atctctgaaa gatatcttcg acggtaaatt gaaggttgat ttctcgccta
    73081 ctatgaaaca gggctttatt atcgctcctg aagatggaac ttataaaatt aaattcgaaa
    73141 gcgatgattt tgccaaccta tttaagagct atttacgccc gcgcacgaag gaaatccttt
    73201 ttggcaaata ataagctatg aaacaagaat attactattt cgtctccgga ttacccaata
    73261 tcataattga cgatacaaaa ctaccttgga ctccggaaga atttttagct gaggcaaaaa
    73321 accaggttag cgaaagtgat tatcgcctcc tggaaatttt gcatctgcca atggatatgg
    73381 ataatctttt gcgggttctg tataaaagca ataaagaaca taataacgaa ggattgtatg
    73441 aggatacttt ctgggaagaa tatctcgttt tcttgcgagc tgttgttgaa aatccggaat
    73501 tgaaaactcc cgttgaattt gctgcccttc cttctttcat taaagagatt attgtaaaag
    73561 tgctaaatca ggaagaaatg cagatcctac ctcaaactga attggaactt ttaaaacaat
    73621 tttatgcttg gactgctgag agcccgaatg attttatcag agactggttt gcctttgatg
    73681 caaatttgcg aaatatctta acggcaataa atggccgcaa attcaatcta ccttatgctc
    73741 aatatctgat tggcgatagt gaaacagtgg aaagactaag taaaagccat gctgctgatt
    73801 ttggtttagg taaagataat gatcttttca atgccctaat tcggatctat gagcaaaata
    73861 atattctgta tcgagaaagg ggatatgata tcgtccgctg gaaatggata gacgattata
    73921 acttcttcaa ttactttaat atagaccgaa ttttaggtta ctattgtcaa ttgcggattt
    73981 tggctcgctg gataaaatcc tcaccagaac tgggaaaaga agtttttaat agcattttaa
    74041 gcgatttgaa taatagcttc agcttccccg cggactttaa tataagaagc actcaaagaa
    74101 aatagaaggt aaataaatat gactacaggt atcgttaaag gaattatctc taacctggtg
    74161 caagtagagg tctctggacc tgtttcccaa aatgagatat gctacattat tcagggtgga
    74221 gtaaaattga tggcggaagt tatcaaagtg gttggtaatg tagctaatac acaggtcttt
    74281 gaaagcaccc gcggtttaaa accaggtgat caagtagaat ttactaatca tatgctggaa
    74341 gtaaaattag gccctggaat gctttccaaa aactttgatg gccttcaagg tgatttaaat
    74401 aaaagggaag gagtttacct tactcggggt gaatatactg atcccttgga tgaagattcc
    74461 ctctggaaat ttacccctat tgccaaagca ggcgataaag taagtgctgg tgattggctt
    74521 ggctctgttc ccgaaaactg ggttaaccat aaaattatgg ttccttttgt gatggaggat
    74581 gaatacacag ttaaaagcgt tgtgcctgaa ggtgaatata aaattactga tacaattgct
    74641 gttttaacag ataatgatgg caaagatata cctgttaata tgattcaaaa atggcctgtg
    74701 aaaatcccta tcacagctta taaagaaaaa cctcgcccct ttaaacttct ggaaacagga
    74761 gtgcgaacaa tggacactct aaatcctatg gtggaaggag gaacaggttt tattccaggt
    74821 ccttttggcg caggaaaaac tgttttgcag cattccatat ctaaaaatgc ggaagcggat
    74881 atgattatta tggctgcctg tggtgaaaga gcaaatgaag tagtggaact ttttgtagaa
    74941 ttccctgaac tggatgatcc cagaactgga agaaaactta tggaacgaac tattattgtt
    75001 tgtaacactt ccaatatgcc tgtggctgct cgtgaagcat ctgtatatac tgctatgacg
    75061 attgccgaat attatagaaa tatggggcta aaagttttac ttttggcgga ttccacttca
    75121 cgctgggctc aggcactgcg cgaaatgagt aatagaatgg aagaacttcc tggtccggat
    75181 gctttcccta tggaccttcc agccattatt tccaatttct atgctcgggc aggttttgtt
    75241 tatctaaata atggagcaac tggttctata acttttattg gaactgtctc gcctgcaggt
    75301 ggaaacttga aagaacctgt tactgaatct acgaagaagg cggcacgctg tttttatgct
    75361 ctatctcaag ccagggcaga tagtaaacgc tatcctgcag ttgatccgat tgattcttac
    75421 agtaaatatt tggaatatcc ggaagtgatt gaatacctca atactaatgt aaatccgcat
    75481 tgggttgtag atgtaaatca tactaaagat attattctgc gcggaaaaga agctcaggaa
    75541 caaattaata ttttaggaga tgatggagtt ccgcttggtt atcatgataa atattggaaa
    75601 agtgaactaa ttgatagagt tattttacaa caggatggct tcgataaagt agatcaatcc
    75661 actccaatga atagacagca atatatgctg gaactgattt tggagatatg taatacgaat
    75721 tttgtgttcg agagttttga agaagttatg ccttatttta ccaggttaat taatatctgc
    75781 aaacagatga actacacaga gttccaaact gcagaattta ataattatga agctgaactt
    75841 cgtaaaattg ttgaggaaag gagagcgaaa taatggaaac ccaggccttt caaaaaatct
    75901 atacgaaatt aacccaaata actaaagcta cctgctccgt tcaagcaacc ggagtcggga
    75961 atgaagaatt ggctacagtt gctggacgtt tagcgcaagt tgtgaaaata gttggtgata
    76021 atgtaacctt acagattttt gcaggaactg aaggtattgg aaccgatgcg gaagttgtct
    76081 ttttcggaaa agctcctacc ttgaaagtaa gtgatgaatt aggtggtagg ttttttaatg
    76141 catacggtga accgattgat ggtggaccag aaattgaagg aaaagaagtt gaaattggag
    76201 gtccttcagt taatcctgta cgccgcaaac aaccctcaga attaattgct acaggtattg
    76261 ccggaattga tctaaataat actttggtta caggacaaaa aatacctttc tttgcagacc
    76321 ccgatcaacc ctataatgaa gtaatggcta tggttgcttt acgggctcaa agtgataaga
    76381 ttattttagg aggaatgggg ctttccaatg atgactacct actctataag aacacctttg
    76441 aaaatgccgg agtaatcgat agaattgtct cctttgtgaa tactacagaa aatcctactg
    76501 tggaaagatt gctggttcct gatatggcac ttacagcagc cgaatatttc gctttggaaa
    76561 aacacgaaaa ggtactggtt cttttaactg atatgactct atattgcgat gctttaagta
    76621 tcgtttctaa tcgtatggac caaatacctt ccaaggatag tatgcccggt tctttgtata
    76681 gtgatttggc aaaactctat gagaaagcag ttcaatttcc cgatggtggc tcaataacca
    76741 ttattgctgt tactactctt tcagggggag atattactca tgccattccg gataatacag
    76801 gttatataac agaagggcag ctttttctga aacgtgatac agatatcggg aaagttattg
    76861 tagatccctt ccgttcactt tctcgattga aacaattagt tatcggcaaa aaaaccagag
    76921 aggatcatcc tcaagtaatg aatactgctg tgcgtcttta tgccgatgct gctaatgcta
    76981 aaaccaagct ggaaaatgga tttgacttgt ccgattatga tatccgagtt cttgattttg
    77041 ctaaagagta ttcggaacaa attcttgcta ttgatgttaa tattagcaca gatgaaatgt
    77101 tagatagaac ctgggctctt ttccagaagt atttcaaccg tgctgaaata ggtatcaaag
    77161 acgaatttat gagtttatat tggaagaagg aatgaatctg aagtttcagt ataataaaat
    77221 atcccaattg cagttaatta aacaactggc agttaggcaa agagcattac caactcttaa
    77281 gaacaaggaa agtgcattac ggttggaagt taaaaaagca cgggataatg cattggcttt
    77341 agatcaaaaa gtgaaagaac gcactgcgga actcgatgct tttatgaaac tatttgtgga
    77401 atttgatcca gacatagtga agataaaaaa tgtggaaatc aaaacccgca aaattgctgg
    77461 cgtaaaaact cctctattgg agaacataga atttgagatt gcggattata acctttttaa
    77521 agcaccttcc tggtttcctg aaggaatttc cttactaaaa gaaatctgcc agctgcaaat
    77581 tgaaagggac ttttttatcc gcaaaatgca tattttggag caggtgcgta aaaagaccac
    77641 tcaaaaagtg aatctttatg aaaaagtgca aattcctgcc ttcgaagatg ctattctaaa
    77701 gataaaacgc tatttggaag atgaagagaa tctatccaag gcgtctcaaa aaatactaaa
    77761 atcccgcttc gaatcggaga ctcgaaaatg attgaaaaga tgcgcaaata cacctttgtt
    77821 ttgtatcatc aggaatacga aagctttctg gttgaactgc aaaaattggg tttggtgcat
    77881 attattcgca gtcgtgatga caaaacagaa tcccaaacca aaaatcttga attgatgcag
    77941 gaatatggtg aatgtataaa attcttaacc aagctgaaat ctattgcccc taaacagaca
    78001 aatcctctac ctacaaaagc attactgaat aggattaatg aagccagaga agaaaaagaa
    78061 aaactgcaac actctatcga tatggtaaaa aagcaaatcc gggatttaac cccttggggt
    78121 catttcgatt acaatctggt tcacaaatta aaagaagcag gtttgacgat ccgttttcat
    78181 acctgtttaa agaatcactt caaaccggaa tggcaggaaa attatcctgt aaagattatc
    78241 aacgaagtgg gtgggattgt ttattttata gtgattactg atgatggcga tccgtgtttg
    78301 gaagcagata ctttttcttt ccataaacat accttgcaag agcttgaaga tcatctatta
    78361 gaactacaaa ataaacagag agatatagat gaatatttat attccatagc gcctacagct
    78421 attgaaatgt tcaatgaaga attaaaacgc ttaagcaatg aatatgaatt tgaagatgct
    78481 atgcaacaag gtgaatttga agcagaaaat acgatccggc ttataagcgg ttggatacct
    78541 gtaagtttag aacagggttt aaaggacttt ctggaaaaga acaatgtaat atattttgct
    78601 gaagaagcca aagcagagga aaatcctccg atcaaactaa agaataactg gtttgcccgg
    78661 ctttttgaac ctatcagtaa aatgtatatg ctgccctatt ataatgagtt tgatttaact
    78721 cctttctttg ctcccttttt tgcgcttttc tttggctttt gcaatgcgga tattggctat
    78781 gggatagtaa ttattatttt ggctgtaatc ttaaaactaa agttgaaaaa caaaggtatg
    78841 aaggacatta tgtccttgct aattatcttt ggcgcttctt ccataataat gggatgggtg
    78901 atgggaagtg ctttggctta tgatctaaaa acgattcctg ctttagcaga taaaatcctg
    78961 ataaaagata ataatcaaat cttcaattat gctctgcttt taggagctat acaaattctt
    79021 ttcggaattg ccatctcagc tgtaaaaaca atacggaaca acggaatcaa atttggtctc
    79081 tctactattg gtaccttctt gttattattg ggtttaactg ttcttgggtc ttcactttta
    79141 ggaaccgata tcagtaaagt tcaaccttac ctgaaatatc ctatttatat aggattggca
    79201 atgattctac tgttcaacag tcccggtaaa aacattattc tcaatattct caacggatta
    79261 tggttgctct atcaaattat aaccggttat tttggagata ttctttctta catccgactt
    79321 tttgctttat gcgtttccag tgctatcttg ggctttgtga tgaattccat tggcagtcag
    79381 atggctggaa tacctgtagt tggcccaatc atttttattc tgtttatgct ctttgggcat
    79441 actttaaata ttggcttggg aggtcttagc ggttttgttc atcctttacg acttactttc
    79501 gtggagttct acaaaaatgc tgcttttgct ggtcctgggc aagaatataa accatttgga
    79561 aaaaaggaat aacataggag gttattatgc ctgattcctt acaaacagta gctcaaccaa
    79621 ttgttgccac tatggcgagc cagaatttag catatttttt ggcttggatc ggactttttc
    79681 ttatggtagc tctttccgga gtcggaagtg cctggggaac tgttatcggt ggtcgtgcaa
    79741 cagttggcgc aatgaaaaaa agggatgatg tttttcccag ctgtatgatc ctttccgctt
    79801 tacctggaac ccagggtcta tatggttttg gttctttctt catcctaaaa gcttatatta
    79861 cccctcaaat taatatgctt caggcttgtg ccattttagg agcaggtttg atgtcaggaa
    79921 ccgtggaact tatttctgct tatcatcaag ctaaaatagt agcaacgggt attgagagta
    79981 ttggttcagg acacgatgtt tttgctaaaa cccttatttt gggtgtgttt cctgagcttt
    80041 atgctattat tgcttttgct gctagtttcc tgatcggtgg aataattcca actttagctt
    80101 aaccaaaatt atcggtcaaa aaaagaaggt gaggtatttt agacctcacc ttctttgtat
    80161 atgcgtataa ttttacaaaa gctcaattac cacgaatttt tgcttgcgaa aattgctcgg
    80221 atcaaggatt gttagcacat tcagattatc ttctttttct actttatagg aagcagcggg
    80281 atgattagtc aacagagaat atcccttctt tgtaacaggg aatctaattt gctggctctc
    80341 taacagattc atagaagtga attggtcaac agagatattt tgttttacaa ctgaaactct
    80401 tccaattccc aaaagaccac cttttctatc cataatccct ttttctatta gttctttgcg
    80461 agttccataa acataataga tagtattggc ttcgatactt tgctggttta gttgttgttc
    80521 cttttccgag atcgtcattt cccttttctg gatttcctcg gcactggttc ttctttctga
    80581 ttccagggtc tcgctcaaaa ttcccaaacg ttgttgcagc tcatccataa tttgttcttt
    80641 ttcgctaatg ctaaccttta gtttattcac aatttcttga acacctttca gctggctttt
    80701 ggaagtggca agttgttttt caagggaggc aattttcttt ttatcagctt caatttggtc
    80761 acgcatattg gcaattgaag agattatctg ggttcttctc tcttcaggtg aagttccagg
    80821 aatttctttc tgggtaaaaa gctgccctga aaggtcttta tccagtgctt ctaaacttga
    80881 ttgaatttca cctattgtgc tcgtagagga ttcgtagagt ttttttagct cttcgttagt
    80941 ctgatttagt tcttttgttt tgtcgttaga atttatccac aaaacagcaa agacaatagt
    81001 cagaattgcc agaattgcta tcattatagc ggttgaagct ttcatgcttg actcctttgt
    81061 cgtattattt atattggcat taaaaattat agcttgtttt tctgtcaaga taaaaggatt
    81121 atatcacaaa gtgaaatact cttatctaaa ggtttggact gatgaaatgg ccgattatca
    81181 gtccggaatt gaaagtatta ttctataccc agaactgtta gttctaaaga caaaagataa
    81241 aaatgccctt tgcatcgttc ttagcagacg ggatgctttt atctttctgc aagataattt
    81301 cgcctctgct tctcagggca aagtaatttg gcaaaagctt gcaaactgcc atcttacaaa
    81361 tataacttta tcgtctactg accgtattgt ctatttggag ttgcagcatc aggatattta
    81421 ccaacagaat attaaatttg tcattattgt ggaattgatg ccaccacagc caaatgccat
    81481 cctagtggat agcgaaatga agattattga tgccattcat aaatatagct atgccgataa
    81541 tccgcagcga caaattttac ccgggcttat atatactcct ccgaaaacct cttttcaacc
    81601 gattttagaa gaagtccaaa atccttttcc tgatggtagt gttacctgta attcctattt
    81661 tatccagctg tattataata aattgaaaca agaagaggaa gaagaacagc gccaaaagct
    81721 atgtattgct ttaaaaaaag agctgcagaa acttcaaaaa aaagaaaaaa tactaaagca
    81781 agatttggaa aatgctgaaa aagcagattt ttggcttgcc tgtgcagaag gactaaaacc
    81841 caatcttcat aacattaaaa ctggtatgcc aagttttact acagttaatt atctggattc
    81901 caatttatca caaatagaaa ttcctttgca gcccgataag aatcccaaag agaacttgca
    81961 actctatata aaaaaatacc ataaagccaa aaaagggctg cagagaataa aagagaattt
    82021 ggaaaagacc aaggaagaaa tagcaaaaat aatagatctt attgccaaag cggagaaagg
    82081 agaactaata gatagcggta ttacttcatc aaataaaccc aaaaacatca tttcggaagc
    82141 tatcctggct gataaacttt taaaatataa aattgatgac gagtttcaaa taatcatcgg
    82201 acgccgtgcc aaagaaaatg attatatcac tacgcaattg gcaaaacctt atgattattg
    82261 gtttcattgc cgtatctatc atggagctca tattttgcta agatgtttaa aaaagacaga
    82321 gcccagacct cagataattg aactttgctg caatttggct gcctggtatt ctaaagctaa
    82381 attttctgcc aatgttcctg tggattacac acaaatccgc tatgtgcgaa aaccgcgaaa
    82441 aagtcccccc ggatttgtta cttatacaaa ttataaaagc gtttatgcta agccgatgag
    82501 tttgagagaa ataaaggaaa tactctcttg aaaaacaaat tgcgagcaat tcttgttaag
    82561 caaagcaagt atagtgaaag tagtttaatt ctgcaatttt tcagccgcga atttggacat
    82621 attggggtta tcgccaaagg catactaaaa aaaaacgaga aacagcaatt attaactctt
    82681 tgtgaatatg aacttatagc ttacgaaccg aaagaacagg gtttgtggtt atatagcgaa
    82741 gcgaatttga ttagcaattt ttctgtttat cccaattctc tttcttgggc aactgcagag
    82801 acaggtgtgg agcttattaa tcaacttatt cttgctcaag aagatataac cacaatatac
    82861 gaccttacaa ttgcctatct aaaatacttg caacaggtga aagataacgc agttttaata
    82921 ttttggcgct ttctaaacag aattactatc ttaagtggaa tcggaaatcc cttaactaac
    82981 tgcggcatat gcaaaaaagc aatttcgact ccttctgcct acctaaaaag taaaggtgaa
    83041 cttatctgcg aagaatgttt aaatgatatc aatatcagtt cagatctgat tatcctatca
    83101 cctttgggac aaaatatctt agccctactt cctgaaatag gtaatcacct acaagagata
    83161 aaattaaata gggcagttat aaacgaaata aacagcttct ttgaactata ttggcaggta
    83221 catcataaac aaccgttaca tctaaaagga ctatccgttt tagctcaatt ctacgattcc
    83281 tgaggtctta cgaagttgat atttgtgatc aaatattaga tatttacttc cagatagatt
    83341 atccaaagag gctatgaggg aagaaaaata taatatcaga atatagtgat atcttaatct
    83401 ggtatgaaca gttagctata tcttgatatg tccagattta tttgtaagct attccttgga
    83461 agttattgcg atgccataaa tcgtatatcg ctaaaatgga atagaatttc tactaatatt
    83521 tgccaataaa ttctcctgca atattgaata tacgatcttt ctccagtttg tttgcaattt
    83581 cagataatat aaatggctag cttaaactca ccttttttta tgctcaactc caaagttatt
    83641 aatcaacttg ggatcagaaa gtgaatatta gggaaaaata ccttgccttg attatcgata
    83701 ggctatattt atatctagtt atttagatgt tgacagccag gaactttcgt tattaagcct
    83761 tcttctacag gcctttttac tgaactgcaa ggtaattgct ttgccagaat ttgtgcttta
    83821 taatagtttt cagattctct tgttcctggt attttaatat cagattattc ccttactaga
    83881 aagtgtctat cacttcttca tcatccctta atcactcctt aattattaac gcttgattaa
    83941 gggatgatga aggaatgata gactgtatca cgcaagggta atgatgatta tcccttttag
    84001 ataagtacca tggcttaatg ggaagaaact gtatagtcag aggacactgt tcctctgctt
    84061 ctcctttaga acatgaaata tctctcacag attatgcaga ttttatttta gaaacaagga
    84121 tttaagaatt ttaggattaa gaggatttct aattctattt ccatttgaaa cgatatattt
    84181 gagtgacagg ttttaaccct taaaaaaaaa cggtatggga aaatatgttc tgtcaaaatg
    84241 acaaaatttg tattctatcc ttcacttttt caggtttgtt taaaactaaa cacggaatta
    84301 ttaatgtaat aatcaccaat actttttcct agtggatgga ctaagggaat tggaagtatc
    84361 ctatactgat aaataggaca gaaaatagaa ttactgcaaa cggtctggca gattactttt
    84421 tcttatttat cagatatcat taagccggat gaagaatcta ttaaacacga gaatattgtt
    84481 cctcggaagc ttcttttatt tttatcttgg atgcctttaa tgccgaggtt gtctttgaac
    84541 ccaataaaaa agttcatttt atcctattct tgggtgaaca gaaaaataaa ttataaagag
    84601 aaaatagaaa ctccaaatgg cttataataa ttgcctcata acgaactata aatttatgaa
    84661 tagctccata attattttcc tgccatctct gagggattaa agaacatcat ttgtgattta
    84721 cgaagataga ggaatattgg aactaagcgt gagaaaaatg taaattatgc cctaataaaa
    84781 tgttaaaaaa ttgttaattg gtcatcaaaa ggaggataaa acaaactcac tatagtgaaa
    84841 ggattatgaa attttctttt ttgatgttct aaagttgttc ccaaaataaa gtacagatct
    84901 ctcttattcg ctaaatattc taatttctca ataactttat gacaagcttc tttttcactt
    84961 ttagtagatt ttaagcaatt ccaatacaga gctccaattt cccaatccaa aatactcata
    85021 ttatgttcag aaccattaat gtctaaaaaa gtgtatttaa aggtataagg aatttttttt
    85081 aacggatttt tgggtgaatc ataaaatata ttaagctgac ggaattgctc ttgatctttt
    85141 gcatcccagt ttctgctaca gggctgataa ttggcagaaa taatagaact cggtttataa
    85201 gttgccaaag agatatattt aggattatta gagtccttaa gtaaatcgtc taaagaatga
    85261 tatacattat gcagaagata atccttctta acatcatagg aagaaacttc ttccaatatt
    85321 tttatctcat catctaaatt agataaatga taactttccg gtctaaaatc tgtacctgta
    85381 gttttctgaa tatcaacttg aatccattga tactttttat actgctcttc taaaggtctt
    85441 gcacggaaag taataggata aatcctaatc cagttcttgt cttcatctaa accagccgtg
    85501 caaactgtct cattatactt tttagaagga ttgggatagg tctttactgc aatgagaatt
    85561 ttcttattca taaattcttt agtgtaaatt cagcagacaa tctcttaaaa actttttttg
    85621 ctacaatgct ccgatggcat tcagctggat ttgcctcaaa gcaggtaagg gcaattcttt
    85681 tatttttcaa agcaagctga acaatctgat ctaagtagtc agagtaattg gtttctaaaa
    85741 ctcccttgta gttcttgaat aaaatctctt tatccttaat tttttttctg tgctctgaag
    85801 atatgcccaa ttcaggaata tgaacatatt caatatccat caggttacaa aaattgctta
    85861 agttctttcc actaaattcc ggtttcatac taattgcatt tgcccttaca tcaatgagta
    85921 tttttatacc tgcaaaaaca agataagaaa gatatttatc aatgcttctc ccttcataac
    85981 caattgtaaa taaagcgggc ttttcgttat tgggtatttc tttctgcaca ttattcattt
    86041 ctgcagccga taaaagtgaa tctgctttac agcttttaat ggcaaattta ggatattctc
    86101 tgtaaacata ttgcattaat tcttgcgtag tgaaaaagcc atatctggat atgatatcat
    86161 ttaaaacctt ttttcgttgt gaagttatat ctattgcatc agcattgttt atctgatagt
    86221 gattctgctt aaaatataag aactcgtttc ttaccaaata atccaaatcc ttttgtaata
    86281 taaacgaata aggtccatat ttatagggaa caaaatcata ggaataagtt tcctgtttct
    86341 ggctcactaa aaacataagc ttctgtaact taactttacc agaattatta agtttagaaa
    86401 ccaaataacg aacttcctgc tgcctcttat ataacatcac cttcacctgc tagccagtta
    86461 tcattttaaa aattatataa atccttgtat aggattccat tttacaggaa tgataatgga
    86521 agtttcattc caaaacccat cttaaattta gtcctttatt ttacaataaa actacttcgt
    86581 agattttagt caactaactt cttataattt taacgaattc gctttcttca aacggctgga
    86641 agtaagttcc aataatcctg catatacttc ttgaatttcc gaattggaca aacccagaat
    86701 tgaaaacact acattgtcta ataattttct atccggtaaa ggattagcat ttctaaaatc
    86761 ataattatta cagtcaacct gaagttcttt aaatatgctt agaatttttc tattctttaa
    86821 actatctaat tgtaaaatga cttttgtatg tgctgataac aattgaggat ttattatcgg
    86881 cgttgattca aactcaaata ctgttttatc taatagtcct tcacctaagc tgtttgttgc
    86941 atttaattct aagtaaaaga aaaatatagt agaatttaaa atagcagaaa gtacattcac
    87001 ttcatcttta taatgtattt cataaaggcg tttatttgct aaaacaccat tattcagata
    87061 taccttaaaa gtatcactaa agcttgaagg agtaataagt tcaggtaatt ctctaacacc
    87121 caaatcatac caattttttc tgccttttac tgaagaaaca ttattaattc ctataatata
    87181 ctttccctta tctttacctt gtttaatata ttttttagct gattcaccca tctcaatata
    87241 ttttaaggcg ttggttcctt ggatttggtg tttctcataa gggcacataa agatccaata
    87301 cttaagttta tctggatcta ttaaaataga tttgcattct ctagggcttt taataactgg
    87361 tttaagaaat tctttctcaa ttttaaaagg gttagattta ggaatataga aaaactcatc
    87421 agctcctgtt ttaactccaa attttatttt agctacatca cccaatttaa tcaatttatt
    87481 ctttgctttt tccataatag taaaatagat gtcaggagct tttatataga tacttcccca
    87541 tttagagccc tgatatttat tttctacatt gttattataa ccttcctcta atagtttttt
    87601 tcttggaatg gcattaactc ttaaatcatc ataatctatt cgtttttgtg tttcttcaat
    87661 agtgaggaag ttttcagtat agagtgaatt ttcaaaagga agtttgaaag cggcaaaacg
    87721 aattaaatca tcggagctta ccattttttg tttagctcca ataacagcga taatagtatt
    87781 tatatctgca cttcggaata ctcttttact caggttatca ataatgaaat agacagggca
    87841 gttttccaga aggaattttt gtaaccaggc accgaattct acatcca
//
LOCUS       DAOSTH010000003        81929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_313_length_81929_cov_10.382759, whole genome shotgun sequence.
ACCESSION   DAOSTH010000003 DAOSTH010000000
VERSION     DAOSTH010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 81929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 81929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81929
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_313_length_81929_cov_10.382759"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1332
                     /locus_tag="PLB65_01240"
     CDS             190..1332
                     /locus_tag="PLB65_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HOQ77180.1"
                     /translation="MEAIKANGINIQKGILFHSASMGELTAIKSLIKKHLQEYPDIKL
                     CITSSTVSSCLEANNISPKVKGFLSVIDIPHLRNRQLKKINPGLICVVETEIWPNMLL
                     WAKRNKVPVLFLNARMSKSTLRGYSLIKFLFKHLQAPITEIHTQSEEDAKRFREIFKK
                     PVLVSGNLKFSIELKDYDAEQLKKEWRYNNDDFIICWGSSRPGEEELLISILPYLKQY
                     ISNLHLIIAIRHPRRLDEVLELLNGLAYSCYTKREIYDPAEDILIIDTLGILDKAYCI
                     CDIAIVGGSFYDFGGHNPLEPAYYKKPVIIGPFHYSCLDSVKKLQQEGGILISDKNSL
                     HSDIITLYKDNELRYNMGEKAKKVLTQNARTLDIYLKALEKWLKEI"
     gene            1332..2195
                     /gene="amrS"
                     /locus_tag="PLB65_01245"
     CDS             1332..2195
                     /gene="amrS"
                     /locus_tag="PLB65_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOQ77181.1"
                     /translation="MRKAMYYTNEGEKIKCLLCPRECILSEGQKGFCRSREVINGSFV
                     ATAYGKTTSLALDPIEKKPLYHFHPGSMIVSLGPNSCNLTCKFCQNWEISQQDCPTRY
                     LAIEDLIEIIKNQQDQKVAFTYTEPLMWYEYILDFAAKAPEIDIVLVTNAYINKEPWS
                     NMMQYVKAVNIDIKSFRDDFYRNICGGTLEVVLSNIIIAKEMGVHLELTNLLIPGYNN
                     SDEELTNLSQFIASVDKNIPLHLSAYYPCYKMTVRPTTREEVEHACEIASKYLTYVYA
                     GNVYSSRYSRS"
     gene            2196..2564
                     /locus_tag="PLB65_01250"
     CDS             2196..2564
                     /locus_tag="PLB65_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HOQ77182.1"
                     /translation="MKKDSILNDLRPADLVLILIVLLTIVFSISYYLSSKPNSQVYIY
                     KDNWLVGVCSLKNDKIIKIDSHNIVEIKKGKVRMKYADCPDKRCVKQGFTNSMPIICL
                     PNKVIIEIRNNGEQKKLILH"
     gene            2572..4572
                     /locus_tag="PLB65_01255"
     CDS             2572..4572
                     /locus_tag="PLB65_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77183.1"
                     /translation="MLRFALYVSNHGFGHSTRISALAEEFIKYGIFCHIISTKPAYLF
                     QNLDPHYFIIHQRSVDVGVKHNENLTVDMERTVSSLLELLSKRNEIIAREVKFLHQEK
                     IDCVIADVPFLVSEFGAYCQLPVYAVTNFEWHYIYSSLLTNNNIINSQTEFTNPVTLR
                     PVLNLIWSLYQRFDACFQLPFSSSESISAFKNNIPCGLLSRKKDSYSDIRLINNWDKD
                     VLILLVIFGGEGKLELDYEALCSAFKGVVISTQTGVQAKNHYQVTMDADFLDLIYNAD
                     FVLCKPGYSTLAEAVQMNKFIIYCPRKKYPEETVLIQGLSKYSNCLQIDTLQMNTKQW
                     KAIFESIHIRKQPVDQYKNNNINVAGSILKKYAQMIGNNLVSVFDLGSNNLNYLLYSL
                     DNKQIIHKTQLTTSLGKDFKNDKLSAKRLQSVKQIIEPLLQFDGYIESKKVLLATGVM
                     RKALNANLLTDWIEKHYEIKSKIISQEEEIRYVYYAARHFQSGEEAYLAIDIGGFSTE
                     FIDLGNDEKIQGIGLPFGLMTLLNEFDVNCDTATKYIQDQLATLTYNGSYHLIGVGLT
                     YTYLAAVIFKFHYNDIDKMEGKTITRSLLQQLFHSIEVKDEDRYLPFLLEESYLPVLK
                     LSILFIISLLDRFNTSEIIVCNSGIAVGYANWLAKTKNKALK"
     gene            4569..5852
                     /gene="miaB"
                     /locus_tag="PLB65_01260"
     CDS             4569..5852
                     /gene="miaB"
                     /locus_tag="PLB65_01260"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HOQ77184.1"
                     /translation="MKFFIETYGCQMNIADSELIASILIEAGHQEVEQIEKADLLIFN
                     TCSVRAHAENRVLGRIQSEKSRKKTNPSLKIGVVGCMAQRIGKELIEENTDIDFVLGV
                     DQYQNLPEIIQEIDNKQYLLDLDSHQIYKGIQPSYHSDYCAYITIMRGCNNFCSYCIV
                     PYVRGRERSRPWQDIVQETIFAGKQGRKDVTLLGQNVNSYFHSDVNFPSLLRKLNEVD
                     EIYRLRFITSHPKDLSDELIEVMAISAKVCEHIHLPIQSGNNKILQAMNRKYTVEHYL
                     ELINKLRNYIPNIAITTDIMTGFPGETESDFRQTLKVLEEIGFDDAFCYKFSPRSGTV
                     AANLPHQISEQERLERLQRMIDLQRNITLKKNRNQIGQTVEIYVESFSKKSTDKVCGK
                     TRDFKMAVLSGTQKDFGTLQKVEVKDATASTLICF"
     gene            5982..6857
                     /locus_tag="PLB65_01265"
     CDS             5982..6857
                     /locus_tag="PLB65_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HOQ77185.1"
                     /translation="MKSMTGFGDAHISKDGIDLDVEIKSINGRFLDLRFILPRELSFF
                     EYKIRKHISNNISRGTVEIRINYNDHREPKLVLDKIKLLKYNELAIQASNLLSSEEVI
                     SIEFLLQEPGVIMSENHLEDDPILANILNECLVLSLQKITESMHKEGEQIKSVLQDSM
                     LKIKNIVASIYELCEPFKKAMFENMHKRILELLESYQVENMEQRLMQEVAIYVDKYDV
                     AEELTRLIAHINTFQNTLQEEGDIGKTLNFIIQEMQREANTLGSKFSTPQSFPLILAI
                     KEEIEKCREIVQNVA"
     gene            6847..7284
                     /locus_tag="PLB65_01270"
     CDS             6847..7284
                     /locus_tag="PLB65_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77186.1"
                     /translation="MLPEPLLKWKSWPFVERPWTSIILIVFLILLSLLLYRITVIGWQ
                     QPFYYFLGMILVFGNLLPYFIITEYELYEDKVQVRYLFIKVSRPYTDFGCFYEDKHGI
                     MLSTFKMPRRLDPFRGLSLRFSSDKKEREKLVEILMQKIGKKY"
     gene            7284..9602
                     /locus_tag="PLB65_01275"
     CDS             7284..9602
                     /locus_tag="PLB65_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HOQ77187.1"
                     /translation="MNTINSKELRQAILDYFSKYPHTGLSYNELVNTFQLTRTGKSLL
                     SGVLNKLIEEGILVKDKRKYHLKTIKTIEKPSPTLNPKLLQGIFDATPLSRNFSYAFV
                     RTPEKDYFIDAEDTLNAFHNDIVAFEPKYRKGHQDYGIIHKIVKRASDHLAGDLTKMK
                     SSWTFVCSNPKVHKWFYVSDTAGAEDGDKVILQVTNWGNPQTGKLPVGRVIEILGKSG
                     DPEVELVAIIRQYNLPLEFPEEVTAAANLLSADIKPEDYHNRRNLTDLYTFTIDPASA
                     KDFDDAISIEKTNKGWRLYVHIADVAHYLPVEGAIFQEAAIRGNSFYFPKIVIPMLPE
                     RLSTLICSLRPQEEKLCLTVQTEFDRKGNILEQHIYESVICSKARLNYDEVDNLFKGK
                     DTDLPQELIEILLEGRELSRLLTEKRMEAGYIFFDLPDIEYEYDESGFVRHLTLAEET
                     ESHKLIENFMLIANEYVAKHLSQVAPTTLYRIHEDPDFNKLERLIELLSYYGVKWVIY
                     ENLNKSLQYLLNSFPNPAYHKVFDRIALRSMKKAKYSIEHIHHFGLAMDYYTHFTSPI
                     RRICDLVIHHLCKTYLCHSSNEKFTLPQLKHYAEVASEQELQADQSERDIERVYSRAY
                     MKKKEGNIYNGMIISVNSRGLIVQLEEIPITGVILEINLEEGDWEYLEREMRYINLRT
                     RKFYQLMDTLKVTVGYVDDDVYFIPLPDTLQHNVPKNMISSATINSLQRNKSAVHKIS
                     SSSNSYSKNKTSRRPISHRSHSNNNQNSRGNR"
     gene            9599..10402
                     /gene="murI"
                     /locus_tag="PLB65_01280"
     CDS             9599..10402
                     /gene="murI"
                     /locus_tag="PLB65_01280"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HOQ77188.1"
                     /translation="MKKAIGIFDSGVGGLTVYKAIRNAFPSEDLIYFGDTARVPYGPK
                     SPNTIIEYSLQNARFLLQQGIKILIVACNTSSAVALPAMQTITDIPIIGVIEPGAEQA
                     AKTTKNKRIGIIGTEGTINSEAYPNAIKKLIPEAVIFSVACPLFVPLVEEGWQDHLVT
                     ELVVKEYLTNLKMQAIDTLVLGCTHYPLLSKAIQQIMGSEVTLVDSAMAITEYLARYI
                     GSESDKQQGSSTFYVSDNEEKFTQIAERILNDNIKKLIRVRLYESWFQN"
     gene            10585..11415
                     /locus_tag="PLB65_01285"
     CDS             10585..11415
                     /locus_tag="PLB65_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HOQ77189.1"
                     /translation="MEPLILTAAITGAETTRSDQPNLPITPEEQAKEAKACFEAGARV
                     IHLHIREDDGSPSQRLERFQEAISAIRKAVPEIIIQISTGGAVGEAFEKRLAPLALKP
                     EMGTLNAGTLNFGNDVFINHPADIIRLAEAFKEYGVVPEVEVYESGMIDSVARLIKKG
                     IITHSPLHVQFVLGVPGGMSGKPKNLMYMIDHLHEEIPTATWAVAGIGKWHLPTSLIA
                     LVTGGHIRCGFEDNIFYHKGVIADSNAQLVARLARIAEEIGRPLATPEQARQIFALHK
                     "
     gene            11427..13670
                     /locus_tag="PLB65_01290"
     CDS             11427..13670
                     /locus_tag="PLB65_01290"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HOQ77190.1"
                     /translation="MLLTENALKVLEKRYFRKDENGIIIEDWSKMLTRVAFNIAAGDE
                     EKAKRFFTLMDSGYFLPNSPTLMNAGGDLQQLSACFVLPIEDSMESIFETVKNAALIH
                     KSGGGTGFSFSRLREANARVRSTNGVSSGPLSFLKVFNAATDAVKQGGTRRGANMAIL
                     NVDHPQILDFITCKENPAELTNFNISVGITEEFMQAVANDNEYNLISPHTGKVNKKLK
                     ARDVFSLIVQMAHKNGEPGIVFLDRINYANPTPELGKIESTNPCGEQPLLPYEACNLG
                     SINLALLNKDGDIDWDKLKEVVYDSVDFLDAVIDVSRYPLPQIDAMSKANRKIGLGVM
                     GWADLLFQLKIPYASEKAVDLGAKLMEFIDYYAKERSVELAKVRGHFPNFTKSIYSQG
                     SLKREKNSQDWDNLKEKIKKEGIRNATLTTIAPTGTISMIADTSSGIEPQFSLAYIKN
                     VMDGEKLIYVNKYFQKALEERGIFSKELVEEILASGSLSGIDVIPQDIKTIFQTAHEI
                     SPEWHIRMQAAFQKYTDNAVSKTINFSNSATVEDIRLSYELAYQLGCKGVTVYRDGSR
                     DNQVLYLGTLATIEEGKEKKVAPRTRPEITQGITRRMETGCGHMYVTINSDSQGACEV
                     FIQMGKVGGCASAQLEAIARLVSLALRSQIKIEPIIRQLKGIRCQSPMWNKGKMITSC
                     GDAVGQALEQFLTLFSNGDINSVDFQVEGDKSDSDRPKGNGFALCPDCGNTIEHLEGC
                     LICPSCV"
     gene            13799..14596
                     /locus_tag="PLB65_01295"
     CDS             13799..14596
                     /locus_tag="PLB65_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77191.1"
                     /translation="MRCKYCNARLAIHDIWCMQCGRHTSALKNELFSIKSLSETYRAY
                     KVNMSSSLPGAAVAVILGLIPVAVLIWMFSSIIVLESNTTLQLLLNLLIKSIVFSIFI
                     PFMLIAFNPICKHNDYHLNLSEMFTALRCYPRYFLWTLINAVFFCIVYIICFGLPNFS
                     SAPILHLVWLILVNYWIAIVLPSLVLMEELSINPWKAIIKSYQHFQDVRWNIYLLALV
                     LAVLNALGCLFFWLLLIPEALILPLSFFAVRDYTRRLIEFELLTYRL"
     gene            14607..15611
                     /locus_tag="PLB65_01300"
     CDS             14607..15611
                     /locus_tag="PLB65_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HOQ77192.1"
                     /translation="MNRREIISLIILIVVIGFGAYKYLTRSFTELKSQYLMDTIVEIS
                     ATSQSKNVGRQIDSVFNFIRSLEDKLNEYDPNSYLGKINADSLNTVFDMDPDMYNLLT
                     IADSLFKMTNGAFDPTIKPVWDLWNFNAENPVPPDSLVLKEMLSKVDFTKIRYDEKRL
                     IKPLGMQLTFGAIAKGYILDKARDHMQTLNLDNGYINCRSSMTFFGYKKPQIVYIQHP
                     RKLDDYIASFKVLNQSISTSGDYQQFYEYNGVRYHHIISPFTGYPVPDVHSVTVLCSS
                     AAWSDGISTALFLLPPETVLDSIKQIADCEAIIYYSKADSLASIKTAGMKDKDLKEKL
                     "
     gene            15608..16378
                     /gene="folE2"
                     /locus_tag="PLB65_01305"
     CDS             15608..16378
                     /gene="folE2"
                     /locus_tag="PLB65_01305"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HOQ77193.1"
                     /translation="MSIPDIQSQPDTRKITIDKVGVKGIRYPIVVQDRENGIQHSVAD
                     LDIYVELPHQRRGTHMSRFLEVLNRYHTEVFIDKLDSFLNELKSTLKADTAYIDISFP
                     FFIKKQAPVSKVVSLLGYDCFFNASYNNNFELWIGVKVPVTTLCPCSKAISVSGAHNQ
                     RSEIFIKVRYREFVWLEELIQLAEEHSSCEIFPLLKRSDEKFVTDKAYSNPKFVEDIV
                     REITMELNKDKRITGFYVEAENFESIHAHNAYALISRI"
     gene            16386..19049
                     /locus_tag="PLB65_01310"
     CDS             16386..19049
                     /locus_tag="PLB65_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOQ77194.1"
                     /translation="MNDKLITGNISFPKPICRNLSNGLEVISVKDNSHPVLCLQLYIR
                     TGSTLENDQQRGYSHFIEHLSFKSTSDFPNNKISLYASGLGGMLNAFTDYDCTCYYLN
                     LPSEKLKEGFHILSQLAFHSTFSKEDVSKEKEIILEEIKQYQNDPESDFLEYIQTNCY
                     QKSPLRYPVLGNPKSINSARYKELREFYKNRYTPVNSFLVICGNYQENELDAYLDTYF
                     SAWENISKPLPYLLDIEPEMNGFRYIFRKKAIGDDTIAFAFPELSEKHPLANALLIAM
                     RYFAIGRSSRLYNRLVEKDKLCSSVKVNSLCGVLSGVSVIYITPISATFIPDIIRIVY
                     EEYSALLQFGIPLAELELIKKDIINSWLFSFEGMENLANLIAAEKFTGDLCRLQKYGD
                     EINSISIEAVMAAIHKYWLPEGITVYHEGSCEISGTKKQAKQIKESLAPKKTILCAEE
                     IHNISIQNLNPPPLYNAENTRRGYITQIDDNYYQIVLSNNVKVIFKQQKNNNISGFSL
                     STPISQISETPSEIGHNFFCTSLQLYKTQKHSHQELLQYSRNNGFNIRLIHHLDTTTF
                     RGKCLSSNLDKALAMLAESIFLPSFDHNYFSVLTSTALDGIRRDNDYPVSYAYMNWYK
                     MLVGNKSNLFRPTGSATQIRSIRIKHLQQWYEDWNIGKDFCLAIVGNHNPQEILELCE
                     KNFGLVHNVSHSPKFKPFYSPSSVHFKKTYRNTDQAIIYIGGFASPAIDRDENTAFYI
                     LSQILGGDISSRFFDILREKYAYAYQTGFDFHSLNELGFWNAYAFCDKDDYNNCLALM
                     KEIIYSIADKGVKENELETAKQYLIGMNRFEDESVSYTASTISNLVALGYEPEYYLLR
                     EERIKKVNSELIWQIAKKWLLPDNQYIYIML"
     gene            19052..19816
                     /locus_tag="PLB65_01315"
     CDS             19052..19816
                     /locus_tag="PLB65_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HOQ77195.1"
                     /translation="MFKLGIDIGSRNTKVVIFNEHTKSIEFSDCKSTEIFILSGVNKL
                     LHNAFLASGINKNELTAIGVTGYGRKLFKDATLTISEISCHTAGCLYYFPDAKTIIDM
                     GGQDSKIITLTDSGKVKDFVMNDKCAAGTGRFLEMTAMRLDCDVSDLSAIASKSTKEL
                     KLNSTCVVFAESEIIGMLSSSTAPENIVRSVHRSIAKRILAQMASLVWEPPIVFTGGV
                     ALNQDIVKCFSESLSYQLITPPDPEITAALGAAILA"
     gene            19816..20559
                     /locus_tag="PLB65_01320"
     CDS             19816..20559
                     /locus_tag="PLB65_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HOQ77196.1"
                     /translation="MKFDYHIHTEDSYDSSIKTIDLIDRAIQLKYDEIAITEHLDLLP
                     IEMKSYGAPSLKHYFKRINDLQNTISELTLLSGVEVGDYHIVKDYAVSLLEGINFDLI
                     LGSVHFLSDQTNVAIPLKKQLSEEQIRDYYLQNLALVSNCDIDVLAHLGVYKRCYNSI
                     PDERIHYPIIQDIFRVIIDRKIALEINYSSLRRGYPSFIPEQHILELYLALGGQLFSL
                     GSDAHFIEHFDLFRDSIPQEFSNSFRYHL"
     gene            20820..21092
                     /locus_tag="PLB65_01325"
     CDS             20820..21092
                     /locus_tag="PLB65_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-only hydrogenase system regulator"
                     /protein_id="HOQ77197.1"
                     /translation="MNERCHILTIIMEDRESAFHPVNELLHSYAPHILLRVGYPMRDL
                     GVAVIFLIVELPIAEMGSFSGKLGQIKSVKVQVTTLKLEKGEKNEH"
     gene            21082..22491
                     /gene="hydG"
                     /locus_tag="PLB65_01330"
     CDS             21082..22491
                     /gene="hydG"
                     /locus_tag="PLB65_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HOQ77198.1"
                     /translation="MNISTEGVKSFIPTTEIEEHLARNRFADTIRIRDIIDKSLDKQR
                     LNPDETAALINVTDPELKQQILEGAHTLKERIYGNRIVLFAPLYVGNDCINDCVYCGF
                     RSSNKECHRATLSKEELISETEALEDAGHKRLIMVYGEHPKYSPEFIADTVQTVYNVK
                     HNKGEIRRVNINAAPLDIEGFRIVKSVGIGTYQIFQETYHEETYKTLHPRGPKSNFLW
                     RLDSLDRAMQAGIDDLGIGALMGLYDWHFEVMGLLYHTIHLEQRFGVGPHTISFPRIE
                     PAIGTNFTNQPPHRVSNEDFKLLIASLRLSVPYTGMILTAREPIEVRDEVLRYGISQI
                     DAGSSIGVGDYAHKDDESKRKSQFVLGDTRSLDDVIYELANSSYIPSFCTSCYRAGRT
                     GEHFMEFAIPGFVKRFCTPNALLTFAEYLYDYASEHTKKVGFDLIDQEVAKIDDPQMQ
                     ISVKDNLAKMKEGIRDLHY"
     gene            complement(22876..23823)
                     /gene="arcC"
                     /locus_tag="PLB65_01335"
     CDS             complement(22876..23823)
                     /gene="arcC"
                     /locus_tag="PLB65_01335"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOQ77199.1"
                     /translation="MQKKAVLALGGNAIIKAGEKGTIAQQFSNTRESLGGIVELIKRG
                     YHLSITHGNGPQVGNLLRQQELGEMEGIPQLPLGVLNAATEGTMGYMIEQSLQNILHK
                     SGIQKQVITIISQVVVDKNDPSMLNPTKFVGSTYYTAAQAEELKNRLGWILKEDSGKG
                     FRRVVPSPYPKEIIPASTINELVDRGEIVIAVGGGGIPIYIEEDGTYEGVDAVIDKDF
                     ASALLALEIKADLFVILTGVEKVYINYGKENQQALDQITIAEAKHYYNEKQFPAGSMG
                     PKIQAAIDFLEGGGSEVLITSIEKIVDAFEGKTGTRFIK"
     gene            complement(23969..25303)
                     /locus_tag="PLB65_01340"
     CDS             complement(23969..25303)
                     /locus_tag="PLB65_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic 2,3-diphosphoglycerate synthase"
                     /protein_id="HOQ77200.1"
                     /translation="MKRKVIIMGAAGRDFHNFNVFFRDNKDYEVVAFTATQIPGIDDK
                     KYPAALAGKLYPDGINIYPESEIKNLIKQHNVDLVVLAYSDLPYEVVMHKASIINSTG
                     ADFMLMGKDNTTLKTSKPLVTVCAVRTGCGKSQTTRTVVKALKAKGLKVVSIRHPMPY
                     GDLVKQKVQRFAELDDLKKHNCTIEEMEEYEPHIMMGSVIYAGVDYAAILKEAEKEAD
                     VIVWDGGNNDIPFYTSDNEIKIVVVDPHRPGDEISYYPGETNVYLADVIVINKIDSAN
                     LYDINEVRENVRAINPTAQIIEAASPLFVDHPEMILGKKVLVVEDGPTLTHGEMQYGA
                     GVVAAEKYGCADLVDPRPWAVGEIKQTFEKYPGVGSLLPAMGYSSKQIKDLEKTINST
                     ECDSVVIATPIDLRRIIKINKPACQVSYELQEIGVPTIADILKNFGKKSTKK"
     gene            25479..25566
                     /locus_tag="PLB65_01345"
     tRNA            25479..25566
                     /locus_tag="PLB65_01345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25513..25515,aa:Leu,seq:cag)
     gene            25811..27151
                     /locus_tag="PLB65_01350"
     CDS             25811..27151
                     /locus_tag="PLB65_01350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOQ77201.1"
                     /translation="MEKKIFVLDTNVLIHNPQALFSFQDNRVVLPIVVIEEIDQFKKG
                     VDEKSRNARQIGRFLDALRKKGSLQEGVPTENGGTIQITINKEITATASELLFMDRND
                     NLIISTALYFKNKYPESIVTLVSKDVNVRVKADCVGINAENYETDTIKYDEFFTGWEI
                     LDVEPEQYREIEEKGYINNIFGDFFANQFVRIPTPDNPDKYTILRYHKGRNQLYVIKH
                     YTGQYVYGIKARNFEQEMALDILLDDSIKLVSLSGKAGTGKTLLAIAAGLQKVVEEQK
                     YSRLVISRPISPLGKDLGYLPGSKSEKFNPWMQPIYDNMEILLSSHLDKNKPNSNTKK
                     RTSVNDLTDYGFLELEPLTYIRGRSLPDQFFIIDEAQNLSPHEMKTIITRAGENTKVV
                     LTGDPYQIDIPYLDSQSNGLSVSVEKFKGEILVGHITLDKGERSALADLAAKYL"
     gene            27155..29044
                     /gene="alr"
                     /locus_tag="PLB65_01355"
     CDS             27155..29044
                     /gene="alr"
                     /locus_tag="PLB65_01355"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOQ77202.1"
                     /translation="MIKKNICDRSWVEIDLSAFRKNLSILKSYLEPHQQFMMIVKADA
                     YGHGAIEISKIASEEGAVYFGVANPEEGRLLRLQGCKSPILILSPCLTSEINVILDND
                     LAVNVTSLNFAKALNKAAVVKDKQAIIHLKVDTGMHRSGILDSEFSDFYSQIQKLSNL
                     NIEGVFSHFASADDDIEFSNQQEERFWKVIDQLPERPKYIHIDNSHALINKLGKRSNL
                     VRLGIMAYGIQIPLQEDLGLQPVMTFKTTLSQIKHISKGESIGYNRSWIADKDCVYGI
                     LPIGYADGYDFMLSNNGVVWLDNVLCKVIGRISMDMICIDLSDIDKPKIGDVATLLGG
                     SRLETRAENLVAKYGGNSYELLCQVGRRAKSYFFQNGKLIHSIPLLRRDFVPDDFPDS
                     KLNKIIESAIAQRLQSIEIGEIIYREMLRDFFYYKDRDIHYRHNFHHKIVFSSSLIAG
                     YYNASTLLNFDKVLSNDYFLVVCASSDEVLRRYFKNGDVEYRWLMDDSFKLDNESFIV
                     CLVQVDELILETSIKHINDCLEIKCSHPKLRGKIGKKVHFTINTQTLYPTSAHQFSVF
                     ITELTRGVDITFQYPQEIKEVDCVPFFSGQDKDPLITQNNREIRVSTELNEWIFPISG
                     VVFAY"
     gene            29142..30092
                     /locus_tag="PLB65_01360"
     CDS             29142..30092
                     /locus_tag="PLB65_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77203.1"
                     /translation="MKRLNLLLMLILCIPLLAGSFNWYSQNDERWKSERLGSGNSSIG
                     SSGCVLSCLSMLLNAEASNQYMTPDRLNEWLQKNGGYAGNNMRWQIPGEIDGSGLGLE
                     LVAQSTKRNDWDFLSGELEKGNKVIVKVNGRRSHWVLVIKQNGPKDNPSSYIINDPGI
                     KEYEERTLAYFGGFKSARSYSGNWLDEQAFDLDSEINVVPVPQDELFLYDVAKLPHPA
                     DVYVTLHNNLPVDITGYFILGLFDEDGNYIRTVDYEYVTVEASGKYDLLYELSDVKPL
                     NEEHQELIIIYSKYFSAMPSLTESLELISKGIRNYTTITN"
     gene            31145..33181
                     /locus_tag="PLB65_01365"
     CDS             31145..33181
                     /locus_tag="PLB65_01365"
                     /inference="COORDINATES: protein motif:HMM:NF017964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HOQ77204.1"
                     /translation="MNNYFMETHHHEWILTNRRGGYALGTGNLINQRKYHSLLVSSDN
                     KFNRTHLVAGIEEKIEWRGEIFNLDSNNYSNCIYPEGFLHLVKPWLRPYPIFLYSALP
                     HQNDILILKEIMMDESTNTTLVKYTNLGHHLLHFELHPKFTMINHHQLNCPGSLDFLQ
                     FDTEIEEFNKFTRYRIHRQDNNVSLYGAVLQGEIIPNRNVYYNVFYPWEVMNGYEGIG
                     DQITLVQISFDLKVGKSNYLLFSDTSIEDAEILIKRIEERYAHLPKPLDYPIFPDSDD
                     ALLNNLDYNDNFLYNYDEYLQILEFALKDFLANDDVVAGYPFYGAWGRDTMIVLNALL
                     HTPNQQETVEKILNKYKGYIKDGLIPNMMVESGKEANYDSIDSTLWLIILLWKLGKLK
                     QTVKYWKDTIAVSEEVLTNILHNDKHPFSVRSDGLIELHSDFAYGTWMDVRIEGKPIT
                     PRNGAPIEINSLWYNALCCYESMCSAYIQKSRKKYQPNDELIMIKNMVKTSLQKFYNE
                     GYLADRIVGEELIMEIRPNAIIALSLPWQVFSQEVLQQVFERSFNELYTNYGIRTLSP
                     NDIRFRKKYYGTQRERDLAYHNGSVWAWLLGPFCGLYERAYSGIKTKEEIIEALTSFI
                     STFRNSFMKGHIASIAEVWDGDAPHFPKGAPAQAWSVAALYNVENYILFLEESK"
     gene            33178..34521
                     /locus_tag="PLB65_01370"
     CDS             33178..34521
                     /locus_tag="PLB65_01370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOQ77205.1"
                     /translation="MKILMFTWEFPPLISGGLGMACYGMVKALLSQGIKIDLVLPTKE
                     LVYFPLRDEGDADTLPAVFIEPEKQIEYTRRAFTCLSERLEYIGVTTHPESYFNMSEI
                     AEYSNLIKKHYELSEVTISEELELLKEMTAHLIGDEDLIRKVQEYTLRANRFAKILEY
                     DLIHAHDWLTYPSGILTKQISHKPLIVHIHATEFDRAGGPGDPRIHKIEYIGMTYADK
                     VIAVSQYTAQMIMSRYRIDSGKIRIIHNAFTLPETNLNNKKRIFIGPTILYLGRVTIQ
                     KGPDYFLDMAAKVLKVHPEARFILAGTGDMQRQILRRSASLSLKNRFLFTGFLNRKQV
                     ERILQAADIYVLPSVSEPFGISPLEAMSYGITAIISKQSGVAEIIHHAFKIDYWDVDL
                     WAETINHLIENPEECRKIGIEGMKEVQRIQWDEAAEKIRQLYATTLSEFSEQRVE"
     gene            34532..35731
                     /locus_tag="PLB65_01375"
     CDS             34532..35731
                     /locus_tag="PLB65_01375"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HOQ77206.1"
                     /translation="MLNIVFYFQVHQPYRLTHFNVLDIGNNAKIFDERLNSNVMRKVS
                     KNCYLPTNQLLLELIQKYEGRFKVAFSITGTAIEQFKLYSPETLDSFKCLIDTGCVEL
                     LGETYFHSLSFLFDTNEFLDQVYMHRELMQKEFGYYTTTFRNTELIYQDRLSDIIYEI
                     EGFKTIITEGVDRILQWRSPLYAYKNYAKNLNLLLKYYQLADDIAFRFSNRDWPEYPL
                     TVDKFVNWIDHLTLAEKEGKNLFLNLFMDYETFGEHQWASTGIFDFMRHFPDAVLSKS
                     HLGFANPKDVSRLANYQQESLSFPEPVSWADEQRDLSAWLGNEMQQNASETLYELINR
                     IKEKGDTDLLRMARLLSTSDHFYYMCEKYFQDGDVHKYFSPYDSPDQAYIYYINALTE
                     LEERLLR"
     gene            35736..37151
                     /locus_tag="PLB65_01380"
     CDS             35736..37151
                     /locus_tag="PLB65_01380"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOQ77207.1"
                     /translation="MKGKILILEDDVVLSEQIANIMRHYEYEVLLTVNSDQFFEILRN
                     FQPDVILLDVYLVGSRLNGIQVLKHLRNNMDLNYKVIVISGEVTSSQINEIRALGAYH
                     FIEKGSAFSINQLLLHIENAITLKKQEEEHIGLQIEYINLKKQYTRSFPFIGESTAIK
                     KVREQIIRLATVDEDIFLIGETGTGKEIAANYYYIYSPRFGKPFHTVNCSALSESLIE
                     SELFGHIKGSFTSADRNKIGFFEECTNGVLFLDEVTNLSLMSQSKILRAIENKEIQIV
                     GGNLKKVNTRLIFASNAGLEKLSDQSVFRKDLFYRIEGNIIELPPLRERDDDILLLIC
                     YFLTNFSSQYSVMDQLDLPALKNDLLSYSWPGNIRELRNFCKFIMINENRINNSIIKK
                     HLSQKISHAHYSGDSNMERYMRIDNLKDSVASFERDYLLHHLQLNEWNVSQTAQSIGI
                     ERTTLYKKIKTLGINQSDMES"
     gene            37153..38028
                     /locus_tag="PLB65_01385"
     CDS             37153..38028
                     /locus_tag="PLB65_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77208.1"
                     /translation="MLQENIGLKVLSLIIAIFVWIQSLLISEQRSVVNLPLALLSIPQ
                     NITLEDFPKTIPFQVRGRGLEIIKLKLSRTKVFIDASKIKPGTDIISLSDYTINLPDN
                     IQLEIIGPVNTQEIAVHSDLFYQKKVPVELTFSDNQTRERFYSMNYSIIPERVTVFGP
                     KSKVHLIDRVSTESITASMLDQSELTLLLNPLGNDVSSSVKQIKVIISSSSNTTRVID
                     NLPITVTQEKKCIPSTVTVKISGDSKEMININPSDINIGIANEPDEYGFYSVTVKIPE
                     GLQLIEVTPQKVRLK"
     gene            38029..39045
                     /gene="tsaD"
                     /locus_tag="PLB65_01390"
     CDS             38029..39045
                     /gene="tsaD"
                     /locus_tag="PLB65_01390"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOQ77209.1"
                     /translation="MIPQYILAFESSCDDTSVAILDTEYNVIVNLISSQPEHIEFGGI
                     LPEMASRLHLRHILTLTKKALDISELNLSDISAIAVSINPGLIGSLIVGLAFAKSLAW
                     SLSLPLVTVNHMLAHIFANCIEHKEIKPPFLAMVVSGGHTELVQFDTLVSFQIVGKTL
                     DDAAGESFDKTAKLLGLGFPGGPIIDDLAKNGNPDFIRFPRALPQKDNFNFSYSGLKT
                     AIRTWLVNQKPEILNDKLPDIAASVQQAIIEPLVNKSVLWARQHKIPYILLAGGVAAN
                     SALRKQVTQSAAKYGIKVFYPSTAFCMDNAAMVGAAAIPKFLVGDYAPLTVNVSSKKG
                     TRTV"
     gene            complement(39127..39202)
                     /locus_tag="PLB65_01395"
     tRNA            complement(39127..39202)
                     /locus_tag="PLB65_01395"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39167..39169),aa:Thr,seq:cgt)
     gene            complement(39198..39866)
                     /locus_tag="PLB65_01400"
     CDS             complement(39198..39866)
                     /locus_tag="PLB65_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77210.1"
                     /translation="MRKIFITIILLVISLCLMAQEVDTLNINFALLNPEIINLELRIF
                     APMQRIYITADFSVDKDALPEATYYSIFLTKDAIIEQLMVNDQFAPCIYASELVPEHF
                     NPIFPFPEMLKEKNNVNCLSFYLPNFSGKINFKLRYSLPIPEWIKESDIRGYVTFSSS
                     QNWFPRNLVSPSKIYARLLSSKNYTMELGTQCTIKEQDGLRTTEGYFIDAPGQQTIFK
                     IIKS"
     gene            complement(39940..42885)
                     /locus_tag="PLB65_01405"
     CDS             complement(39940..42885)
                     /locus_tag="PLB65_01405"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HOQ77211.1"
                     /translation="MNIRWFICGFIFLYANIAIASIRYTTLENFESGSIILSSWSDED
                     VQPDAWTLSTSTPDGSSYCLQLTGNCYKLQQITPYPIDSTNVFQIQVKTTSNPCIQGI
                     GFTDGMHSLFYSFSGTRILDIEVWIPVYQGAFINNVWNTIQLPISNDWQAFWGYHPII
                     NGIIYINDLDGLSSRSVWFDNLIDISSDLPVAPVVSISTSYQVKDTGVFFYNDVIDPD
                     SDVFTYEWSFGDSTYSNLPNPYHLYTIGSSYPYTVTLKVTDDTGKVGFASTDIILETG
                     NSNLPVTCNFVGDIMLARRYENPGGIIPTLGVNAIFNPTKPYFGDAADINIANLEVVL
                     SNVGVHHPTKSVYYRGNPANVSGLVYAGIDIVCTANNHTMDYGIEAYQQMQGFLDSAG
                     IIYSGCGANAYEAYLPAFYNCRGINIAFLRSSDRTGQYNNAQPFLQAGYCKPGFAYMT
                     PYYIQQQIQAVEGIADLKIVEMHGGSEYSLAPGAGYDKELNPFLEDTEDEDYCYRNDV
                     PHQWDREIRHSAIDSGADLVIVHHPHIIQGLELYQNKLIAHSLGNFVFDLDYPETMPT
                     MILYADAYLDGFRNFRIKPFYIDQFIPKPATGQLAIYILDYIASRSRDLDTRVSVDKI
                     ALEAQVLMDDDYPPVVANAFSCHKQLQNLNENMSCTPPIKLPRRGSICEITNVEPPAE
                     YQFRLGQENIWFGNFEDEGSTLWVPPDYSTSDFIDGTRSAKLSTTSSSTVTSTISNRC
                     KIYDNSKKYTLHGWIKTENVYSANIMIRFYTSRTSGSSSYTDYVIDNLSGTTEWTYYF
                     KELNLPSNVYYYDIRLQMTGSTGINGTALFDNVGLIEWTEWSIVDTYVNIPWPNNYYW
                     IQGRTSDTPKSMSFSLLERSYGSSDTSRIVDYEKPKLIFSISPNPFNPNTSINFTLPA
                     AGKTTIKIYNIKGQLVKDLYSGNLEAGKYSFVWNSCDFSNRSVSSGIYFIKLDCGYNQ
                     VIRKAVLLK"
     gene            complement(43489..44496)
                     /locus_tag="PLB65_01410"
     CDS             complement(43489..44496)
                     /locus_tag="PLB65_01410"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOQ77212.1"
                     /translation="MRIAILGGGGWGLALSALLSENGHQVLVWEFNPVYITLLKETHS
                     NPHLLPEITIPGEVDFTNDFKDIVIFSPEIVILATPSQFLRTTLQNTPDSLWHSPSLK
                     AIVNVAKGIEEDSLKTLDIVIKDELPFFPENKICALSGPSHAEEVARKIPTTVVIAGS
                     DEDLLCQLQIVFSNSYFRVYRNSDLVGVEIGGAVKNIISIAAGIIYGLNFGDNTIGAL
                     LTRGIVEIQRLGITLSARPETFLGLSGLGDLITTATSPHSRNRYVGMEIGKGKKLFDI
                     ISQMEMVAEGVTTTRSVYLLAKKMQVEMPIVEKVYQVLYEDEDPRKAIVELMTRELKA
                     E"
     gene            complement(44496..44831)
                     /locus_tag="PLB65_01415"
     CDS             complement(44496..44831)
                     /locus_tag="PLB65_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77213.1"
                     /translation="MVFKKESDPKKIKEFLTLVENPKVELRRYKRPVLVWAVQEGTVY
                     YSFWEDDLLSRYGLENVDGWDFEEDLLFCPDWVEDSDEEDLDDIDLDEDDIEELNRFF
                     HINHDDKDK"
     gene            complement(44847..45998)
                     /locus_tag="PLB65_01420"
     CDS             complement(44847..45998)
                     /locus_tag="PLB65_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77214.1"
                     /translation="MSNSNSKIPSVDTKPDMIKELKEIVDSNESSSSTMQEKRNIPKK
                     HKIRRKTSIRPIIMLLSLCLFLGFIDIDAQITASVKIGATYSDNVFHLSEYDINRFDD
                     HHPNLDFVDTMDDLTLSTRIELGYPMSYRWWKFTPSITSTLSQNISNEDKYRYDWAAK
                     FRVDRYYWNCSIQYASSPYIYFRHFIDTDGTKELEKYNYSRDTFRGDLAVKPFPKTTL
                     KADLRYDIYRYNEYFTEADGKAFAEELGVSYKFPYFTIEGSYAYRDFSCDKRIENKDC
                     SYQSNTYKGKIVLPKMPLESKSETLWQPSLTLNYEQKYYQGGGSWYGGRADYTYTLSA
                     GMNILFSPKWNLSLDYSHLFRNVETDNESVLRLKEYNENRLSAALRFKF"
     gene            complement(46008..46829)
                     /locus_tag="PLB65_01425"
     CDS             complement(46008..46829)
                     /locus_tag="PLB65_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77215.1"
                     /translation="MHKHHFSIILIILFANILFAQTANRYKVLPFDNPGTKRLLKTET
                     GNYYYYRSLPERALILNTTGVDKIEIRSFSYGEVRKPEIVSVIGKERKVYQLSLKEKK
                     GDYYIYNPFTLDIPEGTKEIKVICYDRSIYMRAFSVLPPKPAKPKKQPNLVIKAHSGV
                     VSVQHNSSSTDYYSFMPDQPFKFTINNGRNAYVYIRPRLLDRSLPQLGVYVNGELVEV
                     IDFTYKRTTKYHSQGINNLGIAKKIVLPENTNSTNYELRALSDHLFLAKPVLLKK"
     gene            complement(46842..47963)
                     /gene="pgsW"
                     /locus_tag="PLB65_01430"
     CDS             complement(46842..47963)
                     /gene="pgsW"
                     /locus_tag="PLB65_01430"
                     /inference="COORDINATES: protein motif:HMM:TIGR04332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate system protein"
                     /protein_id="HOQ77216.1"
                     /translation="MYRPSLKSDRSLIILLILAIVLFYIAQSSYLHIKSDNYELKIAA
                     ANLMKASIDSLTAEINRRGIEIDPIDDPLKTGLIGNRLSSITTDRGLLSEKQAAINPN
                     ISAIFVEELSKLKLMPGDCIAVGITGSNPAVNIALYSAIQVMELNPKIIVSLSSASYG
                     ANRPELTWLDMENILKEKGLINFKSAYASFGGKEDLGIGLSDNGIKALRDAMQRNNVP
                     LLIGNNLDDNISLRMAAYDEMLPEGQRYKAFINIGTGIANVGSEPNANLIPEGINRNL
                     AEHSYEKEGVMIKMAKNNIPVLHFRRILRWAKRYGLPTSFDTIPKVGQGKMFSSLVHN
                     QTVNIICLAILVIAIAMVIIFDRHDRRFMANIVDPDEEL"
     gene            complement(47966..48421)
                     /gene="pgsC"
                     /locus_tag="PLB65_01435"
     CDS             complement(47966..48421)
                     /gene="pgsC"
                     /locus_tag="PLB65_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein PgsC"
                     /protein_id="HOQ77217.1"
                     /translation="MIDTIVQAAVGMGVIVSLMFSELLGASAGGIVVPGYIALYLDKP
                     LQIIGTLIISLITWGIIRIISSFTLMFGKRRMVLSILIGFILGWASRIFVFQDITVHQ
                     LQMQSIGYIIPGLIANWFERQGFWKTVSSMGIAAILVRLALMVIFNGEI"
     gene            complement(48418..49608)
                     /gene="pgsB"
                     /locus_tag="PLB65_01440"
     CDS             complement(48418..49608)
                     /gene="pgsB"
                     /locus_tag="PLB65_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR04012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0070501 - poly-gamma-glutamate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate synthase PgsB"
                     /protein_id="HOQ77218.1"
                     /translation="MTVLIIVTLILITHWIIEYQRHLKNVRAIPIRIHVNGTRGKSSV
                     TRLIAAGLREGGKTTVAKTTGTLPRVILPDGREAAVIRLMGANIIEQKYIFRHAVKHN
                     PDAIVIECMAVNPVLQWITERKFIRSTISVITNCREDHLDLMGSTVQSVTMCLSNTIP
                     KHGVCYTAEDEQFSLLKKVAEQRRCRIHKIRPMDVTQEELDKFRYIEHAENVQLALAV
                     CAEAGIEREIALHGMQKATPDPGALTKYKIQDGNKTLFFYNVFAANDPQSTVGIIKMV
                     TGHLQNVEKIIILNSRADRLFRSHQLVDAVKDLDFSYLLLTGEIPEKVESYCLQAGIP
                     KEKIIPLGEPLPEVIYKKVIELTNSESHILGIGNIAGDIKYGAQIVAHFKHKMKENIE
                     RSRM"
     gene            50215..51147
                     /locus_tag="PLB65_01445"
     CDS             50215..51147
                     /locus_tag="PLB65_01445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ77219.1"
                     /translation="MKIATSPKVLYYHIVADSFPEIYPQGISVSSFFTQLRAFQQKGY
                     NFRPLSEVLADPQQFDGKDLVISFDDGFAVNYPVLMYLSKEYKIKPTLFLIGKCIDNR
                     EMAWNHKLILLKQYCSSEKLALIIDNYVPGATPASLFSCVSMSDKDEIANSLWNEIMP
                     FSEKDYLNNHKPFLSISQLESLLDAGVELGIHSWSHPDFSRLSYIEAYNELEQCFSLM
                     EEVNLPCHRYFAYPYGRPAATAVESKLIEKIKLSATFGISYKLGDNRSGDSRWQRTKM
                     DGSKIMNWEQFYLSPYLRLLKHPVHALKDVLEFL"
     gene            51236..53587
                     /locus_tag="PLB65_01450"
     CDS             51236..53587
                     /locus_tag="PLB65_01450"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOQ77220.1"
                     /translation="MTSIPVLWLIAPLGAVLALIFAAVFFYQVKKQDPGSTEMQEIAG
                     YVREGAFAYLKQQYKGVGIFFIIAFIIFQIMSLGLGVLHWLIPWAFLTGGFFSGLAGY
                     IGMNMATLASNRTTQACTVGLNKGLRIAFRAGSVMGLTVVGLALIDISAWFLILNLFH
                     FPLSQITVIMLSFGMGASTQALFARLGGGIFTKAADVGADLVGKVEANIPEDDPRNPA
                     TIADNVGDNVGDVAGMGADLYESYAGSILATAALGMSAVNMLGANYASWATNFVVAPM
                     ILAGLGALLSILGIFTVSTKENAGPKELMFALNKSVYSSSLLIAICAFFVTKSLLPPD
                     YYFGVFISTVIGLVAGILIGLFTELFTSHSYKPTREIANQAEYGPATIILEGISVGMR
                     STAAPVIVIVVGILAAFICSHGFSSIEMGLYGIGFGAVGMLATLGVTLAMDAFGPIAD
                     NAGGNAQMCNLPENVRLMTDNLDSVGNTTAATGKGFAIGSAALTAMALLAAYLEEVRG
                     ALTLIGNKAGKAVFLHTNYNMKFSENIPIAEASMADFIHYFQIHVLNPKFLLGIFIGA
                     MVAFLFSSLTIKAVGRAAGKMVKEVRRQFKEIPGILEGTAKPEYAKCVTISTQAAQKE
                     MLLPALIGILSPIATGLILGIPGVLGILVGALSSGFVLAVMMNNAGGAWDNAKKYIET
                     DLKAKGTDIHKSSIVGDTVGDPFKDTAGPCINILVKLMSMISIVFSGLIVSYSLHVEN
                     INPPFSKAQLNHKIENKIIMVDKDSLNVTNIYPVSAPKIPQDQ"
     gene            complement(54365..56149)
                     /locus_tag="PLB65_01455"
     CDS             complement(54365..56149)
                     /locus_tag="PLB65_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HOQ77221.1"
                     /translation="MAKTVNVFIDGHHLEVPDTMTIIEAADKIGIYIPRLCYHPDLPP
                     TGNCGVCVVEISGNPTPKRACCTPVAEGMKIVTNSKKLRTYRKTVVEMILSNHDVTCP
                     TCSANNKCELQSLANNLGVDPDALPSILVKKPADDSSLSIVRDTNKCISCGRCITVCN
                     EVQTVCALTFADRGIDTHVDTAFSNGMANSPCVNCGQCTVYCPTGALHERSELDAVWE
                     AIFDPDKHVIVQEAPAIRVSLGEEFGMPLGSVTPKKMYAALRKIGFDAVMDTNFTADL
                     TILEEGTECVTKLKAGEKRPLITSCSPGWIKFMETYYPDLADCVSTAKSPMSMFGVLS
                     KTYYAQEKGIDPAKIVSVAIMPCTAKKFEARRPELSDSGYQDTDYVLTTREFIRMIKE
                     TGIDFANLQDEEPDAGMSFYTGAGTIFGATGGVMEAAIRSAYYLVTNTDLENVDITAV
                     RGLEGVKEASVDIPGFGKVNVAVAHGLSNARKVLDQVREGLATKGESPWHFIEIMACP
                     GGCVGGGGQPYGNDIASRARRGLALYEEDRNLPVRMSHHNPEVLKIYEKFLGEPNSKL
                     ALKLLHTHYFKRSIFNGQITEEITHKHQ"
     gene            complement(56171..57733)
                     /gene="nuoF"
                     /locus_tag="PLB65_01460"
     CDS             complement(56171..57733)
                     /gene="nuoF"
                     /locus_tag="PLB65_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOQ77222.1"
                     /translation="MKYYRNHILVSINETSLAAGVQEFISSLRNELAKNDLAEEINIL
                     ETGPLGFFGRGICLTVYPENINYEDVKIEDIPELVQEHFLKGRPLKRLMLGAAEKFSP
                     KFNYENRIVLRNSGIIDPENIDDYIGTGGYEALEKALTQLRPEDVISEVKKSGLKGRG
                     GAAFPVGLKWSFTAALNVPQKYVVVNADEGEPGTFKDRLIMEGDPHQLLEGVMLCAYA
                     VGANKAYIYIRGEYKLCIARLENAIKQAYQYGILGKNIFDSGFDIDIEIKIGAGAYVC
                     GEETALIESLEGNRGNPRWKPPFPGVEGLWKAPTIVNNVETLANVPFIIAKGAEEFVK
                     YGTPECPGTKVYTILGDVAFPGLCEADMGTTLRTIINDYAGGMKKGFRFKAALVGGAA
                     GVILPDRLLDVKMDFSSLNQYAAVLGSGAILVLNEHQSIIDLLWSILRFFRHESCGKC
                     SPCRNGTEQLYKLISKIRKGNGTMQDVDLMLFIAETMQQTSFCALGQSPIMAVRSAIE
                     NFPDEFIELTKK"
     gene            complement(57733..58188)
                     /gene="nuoE"
                     /locus_tag="PLB65_01465"
     CDS             complement(57733..58188)
                     /gene="nuoE"
                     /locus_tag="PLB65_01465"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOQ77223.1"
                     /translation="MIKEICKKYAPHKDNLIQILHEIQNADPQHYISNEAVDTVAEYL
                     NIPANHIYGVLTFYTMYSTKPRGKNIIRLCESPPCYIKGSENILRKLKVLLGVNVGET
                     TKDGLFTLELCACLGVCGNAPVMMINDDFYGDLTEEKVEEIIDKIREMR"
     gene            complement(58202..60061)
                     /locus_tag="PLB65_01470"
     CDS             complement(58202..60061)
                     /locus_tag="PLB65_01470"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOQ77224.1"
                     /translation="MSLKRIDLLICCGSGCVSAGAIKIKDQFHSVLKDKGLTNEINII
                     ETGCMGPCDYGPVMVIYPEGVFYKKVTPEDVEEIVEEHFLKGRPVKRLMLQDEEEKNY
                     LTNKEIPFYQKQVKVALANCGYIDPESIEEYIATGGYEALGTVLTTMTSQQTIDVIKK
                     SGLRGRGGGGFPTHLKWQMVHDNPSEEKYFICNGDEGDPGAFMDRSLLEGDPHRVLEG
                     MMIAAYAIGAKNGFFYIRAEYPLAIKRIKLAIQKAKEAGLMGENIFGSNFSFEAEVRT
                     GAGAFVCGEETALIHSIEGQRGNPTPKPPYPAVQGLLKKPTIVNNVETLGNIPTIILK
                     GAEWFSSMGTQTSKGTKVFALTGDVRNTGLVEVPMGITLRELIFDVGGGIIGDHKFKA
                     VQLGGPSGGCLTTQHLDVPIDYESLKERGAMMGSGGVIIMNEEKCMVNVAKFFMDFCV
                     DESCGKCAPCRIGLKQMLEILKRIDSGNGKEGDIEELQRLGETISKLALCGLGQTAPN
                     PVLSTIRYFRDEYEAHIRDKSCANKVCLDLMHFEIDKSRCIGCSLCSRKCPTKCISGS
                     REDKYTINQLSCIKCGTCMDVCPVKAISKVPGMHPDVKAKREAEAIKQQNQDE"
     gene            complement(60080..60445)
                     /locus_tag="PLB65_01475"
     CDS             complement(60080..60445)
                     /locus_tag="PLB65_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOQ77225.1"
                     /translation="MKTLADLKKIREKAQEEMKLRGGTTRIKVVVGMGTSGIAMGARE
                     VMKTFLEEIANRNLTDVLVTQTGEKGLSSLEPVVDIIEEGKPKITYGNMTPEKVKKVI
                     VEHIVNGRIVSDYVVATGN"
     gene            complement(60445..61014)
                     /locus_tag="PLB65_01480"
     CDS             complement(60445..61014)
                     /locus_tag="PLB65_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ77226.1"
                     /translation="MQDISLHLLDIIENSVRAEAKLIKIRIIRDVKDNLLRLIVEDNG
                     IGMDSQTLEKAQSPFYTSKKDREKKVGLGIPLLKQNAEATNGSFQMTSAPGFGTVLTV
                     DFQLDHIDRMPLGNLKDTLLGVIIGHPEVDFSIKLISHEQGVEKSFYFETAAIKEELG
                     NIPLTYPDVIEYIDQSLLEGIQNTNMEDV"
     gene            complement(61028..61510)
                     /locus_tag="PLB65_01485"
     CDS             complement(61028..61510)
                     /locus_tag="PLB65_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOQ77227.1"
                     /translation="MTSAPQMHNDLYNQLKEVIAEKKNVRNPLIEILRVAQDIFGYLP
                     IEVQEFIANEMNIPASHIYGVVTFYNFFSMTPRGKYNLNVCIGTACFVKGAPRLIQML
                     SDELGIQMGETTKDGLFTMTAVRCVGACSLAPVFVIGEDTFGRIDSKDKVAEIIKRYQ
                     "
     gene            complement(61542..62279)
                     /locus_tag="PLB65_01490"
     CDS             complement(61542..62279)
                     /locus_tag="PLB65_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOQ77228.1"
                     /translation="MLSLRADLHIHSVLSPCGGLEMSPKAIIQRLKYFGIQWMAITDH
                     NSMQNCPAYEMVAKNEGLYYSWGVEIQSIEEIHLLAYFDDSSSAKEFDSELYNSLLPI
                     NNDVDFWGDQVIIDENENILRVEQRALINSSQWDLNTVAEKVQAYNGLVVPAHIDAGV
                     NSIISQLGFMPSEPELKLFGITAMLDIKSWLTEHPDFEDKVFLRASDAHYLNDIGKGY
                     SIITVAEATVQELILAAKGVSGRKIEV"
     gene            complement(62291..62632)
                     /locus_tag="PLB65_01495"
     CDS             complement(62291..62632)
                     /locus_tag="PLB65_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77229.1"
                     /translation="MINLSDYVTAIKGINCTSALSLEGVKITGAYVCDMLSDVMGSAK
                     PGQVWITIMKHLNIIAVASMTGIPAIIFAKGNEPDTAVIEKAIEEGIILISSKLPTFE
                     LAGKLYKLLNP"
     gene            complement(62633..63934)
                     /locus_tag="PLB65_01500"
     CDS             complement(62633..63934)
                     /locus_tag="PLB65_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HOQ77230.1"
                     /translation="MTESIYFHALHILEDNCTGCTACVRVCPTEAIRVRDHKASIDPY
                     RCIDCGNCINICRFHAIIPICDSLEIIHNFKYKLAIISSSFFGQFTEDISYEVAKQAV
                     LDLGFDQVAEEASVTDFMINIIRDYIRKNREKRPILSSNCPSVVRLIQLKYPSLLPSL
                     FHQEAPMSILTRYLRDKISQEYGLEEKEIGIFLIVPCIAHVTAVHQPEGAYKHLQDGA
                     FSIGMIYGKVRDSLKYLQNNPPSIDTYPKGLTWALSGVQADLVDCDDIRALSVNGVEN
                     VMEILSRVEDHYLDQYDYIVLRTCTNGCVGGCLNVENPFVAMSRIKKMIKEGKEEDYD
                     TSELYELYEKGEFTVVPLAPRPIMELDKDIKKAIQKMKQINEFLTMLPGLDCSACGSP
                     TCYALAEDIVLGKASIDDCVVLLKRHSKDTEEDEDKKNKDT"
     gene            complement(63939..64463)
                     /locus_tag="PLB65_01505"
     CDS             complement(63939..64463)
                     /locus_tag="PLB65_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ77231.1"
                     /translation="MQDFVAAKFEMAEYWHINSGLEERVKEFLAKKIEPDVVLQIDIP
                     EKDFNIAGRGSSELKNLLKRLGVDSDILRRIAVASYEAEINVAAHSIGGKMISNVYDD
                     LIYMQFIDNGPGISDIEQAMIPGYSTADDLVREFGFGAGLGLPNIKKNSDALHIESAL
                     GSPTLVEIIIFFLK"
     gene            complement(64435..64782)
                     /locus_tag="PLB65_01510"
     CDS             complement(64435..64782)
                     /locus_tag="PLB65_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HOQ77232.1"
                     /translation="MTLYDIVELLDAEVLYEGADLNKVVPCAFASDLISDILMCTKEP
                     TLLLTGLTNNQVIRLADMIDLIGIVFVRGKKPMQDVIDMAKERGLPLIATKMTLYRSS
                     GILYNAGLRSCKI"
     gene            65220..65900
                     /locus_tag="PLB65_01515"
     CDS             65220..65900
                     /locus_tag="PLB65_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HOQ77233.1"
                     /translation="MELQYFGHSTFLFTNSQKQNIIIDPYLNANPLSPVKSNQVKADY
                     IILTHAHSDHMADALTIADRHKTTIICVSELASIISKSGFRTHPLQIGGAFRFDFGRV
                     KFVPAFHGSSTMNGVYAGLAAGAVFTVEGKTIYHCGDTGIFGDMKLIGEIDKIDILLI
                     PIGGNYTMDIDDAVRAVTMINPELAIPMHYNTFDLIKCDPQEFIDRIATNGFRGKVMK
                     PGEKLNIP"
     gene            complement(65971..66885)
                     /locus_tag="PLB65_01520"
     CDS             complement(65971..66885)
                     /locus_tag="PLB65_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOQ77234.1"
                     /translation="MMSRKAIIILIIVLLIITACGKNTVLNTPDIDPSSINFGTPQTL
                     DVITWNLRTFPQGTDLEALKQIILALNVDVIAFQEIMDYTTFIDMASDIPNYNACIYS
                     ATDTYRLAYLYDTRTVTVNNQYTIYENDSNPFPRPPYILDITWENDNYLIINNHLKAF
                     GDNYIDENNNWDEEVRRRLACMKLDSYISTNLPNSKVIVLGDMNDQIAEPPEYNVFLN
                     FINKSDEYYFVDMPIAVSPNYNNVSYPNSFSHIDHILITNELFADFEQGGSYCKTILA
                     ENWMLNWNTYSSTISDHRPVGIRLGKPH"
     gene            67031..70555
                     /locus_tag="PLB65_01525"
     CDS             67031..70555
                     /locus_tag="PLB65_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ77235.1"
                     /translation="MKKTIFILILLACSILAATTIQNFVTSGSSLYAQFEGLQTNPIE
                     YQDTNMEEDEPFASGSFITKTFAFPYADAELIISSMCWNVYDSEGNFLYSEQRKENSA
                     VSIVNSFTFREMRGYTIRIETQREEDGLIHTLNELSFELTGSNPIQIPDSVSPAFIDA
                     YKALADNFDYSYLRELPISRPKILIISHSQLANYQNAYISWKKSLGFDVYTVNKSDIG
                     SSIQQIKTYIANHYQTYKCDYLILWGDVTGTFAIPTNYFPSPEYAENDADDNYYAMLE
                     GDDYFPELLVGRFSFADASEFITMINKTIAYEKAPYMTDTNWMKRSLMVAGNYAEGGL
                     RPSTPISMSRWLRNRMLSYGYAQVDTVYFPPSFPGTTAIQQSINQGVQFISYRGWGDA
                     NGWHYPQFHIPDLNNTFNGSKMPIVFSIVCNTGDFANTVNPNFGERWMRMGTMSNPGG
                     CVAFVGPSDLHTKTRLNNSISSGAFRSIFDYGVRGFGTSVMIGKIELYKNFPNDLAPN
                     QYVAFYYHVYNILSDPTLNMWVLVPNIISENVIEGGLTYNQSDNHIRINAPHLNSGII
                     SGTKDGINYSYAPIENGYGILPIDPNVEGELTITISKKNYVPLVRTLTLQQNSTIGIV
                     SNNLGNTILAPDTDYSLSLQLKNFSEQIYNNVNANLTTNHSAIILSNPTVSIATINPD
                     ATVSVEFEFTTTGDFRPGEIIDFVLSLDNPSETHIFQLVTGGAKIHLVNYEGILEIGE
                     ENNIIFSVTNLGNYPMEDINLNIQSLSTAAEAQSNSISIGSLAPNETKQFTATITIME
                     GVTPGHNIPLRIDATNNTDYSYYSFYAVTAGNPGANNPTGPDEYGYYAYDSNDLGYAQ
                     TPVYDWIELDPEDGTPIGNVYLNRDDGVKTITLPFSFRFYGVDYNSLTMSTNGWVSFI
                     PTDDSNFYNCYIPAALGPYAMIAGYWDDLKGMKTGEDENGDPVYDYMHIIYWYDSQNN
                     RFIVEWNKAYNQYTIDLGPNASLEKFQIILYPKPDQDGDIVIQYHTVDNPGITTNYCT
                     VGIEDHNQLSGLTYTHGNTYPVTANILAPELAIKFTTNKPDNFVSNEDNTLPEPISNL
                     INYPNPFNPTTTIAFNVKQPAKVTINIYNLKGQLIKTLINNELLYGEHKVVWDGTDNL
                     GNNIGSGVYLYKMQLNGYSKTKKMLLLK"
     gene            70809..71453
                     /locus_tag="PLB65_01530"
     CDS             70809..71453
                     /locus_tag="PLB65_01530"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HOQ77236.1"
                     /translation="MIDVIVFLGIQGSGKGTQAELLAEKTGFQHINIGDLFREQISKQ
                     TELGKKVQSIIAKGELVNDELVFELVNSSLSTSCPGVIFDGFPRTQAQAEYLLQHYNV
                     LRVYYLELTETEAIERIEARRLCSSCGATYNILHKKPTREGICDVCGSSLIQRTDDNG
                     EAVRKRVNEFYKQTFALKEYFEVMDLLKEIPARDSIEIIQQKIITDLQTKLKIN"
     gene            71455..73149
                     /locus_tag="PLB65_01535"
     CDS             71455..73149
                     /locus_tag="PLB65_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HOQ77237.1"
                     /translation="MPSANKPGFFKRIELVAYLMAIWSCLVLFLEPIFEYYVGSYQTI
                     EFITALANILLLMLTILSRVLSGEVQQNRTVVYFDLVILILGSLLLIYQAKFVIFFLL
                     IRQTWFIVQFLLFRAFEGKIYKLLMRNPPISLMLSFAAVILIGTILLMLPASSTEGYI
                     THFVDALFTATSATCVTGLVVMDTGSYYSLFGQIVILLLIQIGGLGIMTISTAFALML
                     GQKISLNVQTLMHKVVGETPVIDVLKLLRSTVLVTVIIEIIGAVFLFIGFAKEMPTAQ
                     AIYYALFHSISAFCNAGFSLWSNNLCSYVQSPLVNLSITFLIILGGLGFAVLIDIYHY
                     FFAKGIIKKLTLHSKIVLSTTALLLISGFLGLYISEYNSSMKGFTIARRVLGSWFQSV
                     TARTAGFNTIDISLYSSASVLITVILMFIGASPGSTGGGIKTTTFAVLILSVVSMLTG
                     KQDLVIFKRKISLSNARESTTLATLALTIIIVILFVLMLTEPFPFEQILFEAFSAFGT
                     VGLSMGITAKLSYIGKLLITLLMYIGRIGPLTLIYALSIRNLQPRVTYAEETIPIG"
     gene            73181..73885
                     /locus_tag="PLB65_01540"
     CDS             73181..73885
                     /locus_tag="PLB65_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HOQ77238.1"
                     /translation="MARYAVIGLGRFGMTVAKILAENGMEVIAIDKNKDLVNEVSTFV
                     TSAVCMDSTDEAALRSQNLNETDAVIIGIGSNVEECILTAAILKKIGVGIIYAKVESE
                     LHGRILKLIGVQHTLLPEEMVGNQLAKTLISRNVLEYINLSSGHIVVEMIAPKEFVGK
                     PLQELALPSKRGVNVVAIKYNYLSVTEDGRNVIEKRLNDMPGANDIINEGDVLILLGP
                     KGNIDKLIYETAVNKD"
     gene            73891..74628
                     /locus_tag="PLB65_01545"
     CDS             73891..74628
                     /locus_tag="PLB65_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77239.1"
                     /translation="MKRSLKAKYHTMIAILFGIIVLQTVILFFAVAQATDLTTLASDI
                     HSIVIIFVFIIFVYIVVLYNYIPYRLHKAIHSVQQLVEEISSGNYQIDIDSSIYDQDK
                     DFQDLIFALEKMLGIITRFDQAKADKVFEHHQRIQFLINLLPQSILIASSNGDVVYCN
                     EPLRKRFPMISETVNLNELIWKDDYHARVFSVIIDALRYGNNLYNVIVEDVVYLHRAR
                     INGSIIRNRKGLSTGAVFVLDFFDNER"
     gene            74618..75652
                     /locus_tag="PLB65_01550"
     CDS             74618..75652
                     /locus_tag="PLB65_01550"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOQ77240.1"
                     /translation="MKDKIRVVYAEPETQRLDKYLVALKIQELYSRTFIEHLIEEDCI
                     LVNLTPVKKSYLLREGDEILIHLPEPEPIDMQPQDIPLDVIYEDEHLAIINKEAGMIV
                     HPGFGNPQGTLVNAILHHFKGNLSSGRECNRPGIVHRLDRGTSGLIIIAKNDPTQSYL
                     ADMFAKHKIKKTYLAITSGVPDPLNGDIETYLARSNKNPRMICVANQGRYSFTHYETL
                     KTWHYFALVKVIPTTGRMHQIRVHFAHIHYPLLGDLLYNSRRYVHSLVPQNMKRKVTE
                     LLVNHLHHQALHSWKLEFEHPITNCPISVTAPIPEDIKYTLNWLDAYFCVDTDTNNLN
                     LLLEEETKIG"
     gene            75645..76877
                     /locus_tag="PLB65_01555"
     CDS             75645..76877
                     /locus_tag="PLB65_01555"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOQ77241.1"
                     /translation="MDKKEQQIDKTLFASIEEAIDIIRNGGMLIVVDDENRENEGDLL
                     MAADKITPEMVNFMISEGKGLLCVPLDYEIASRLDIPLMTNKSSDRQGTKFAVSVDVI
                     EGTTTGISATERAKTIKALANHNAIASMFMRPGHIFPLIAERGGVLRRAGHTEAAVDL
                     TRLAGLNPVGAICEIIRTDGEMARLDDLIPFAQKHNLKIITIESLIHWRRKKERLVHP
                     VSRAQLPTKFGQFEIITYISDISDEYHIALVMGEINPSIPILVRVHSECLTGDALFSK
                     RCDCGEQLAHAFQMISEEGSGVILYMRQEGRGIGLLNKIRAYHLQDEGADTVQANLKL
                     GFPPDLRDYGIGAQILKDLGIKKMRLMTNNPKKIVGLSGYGLEIVERVPIEIKPQENN
                     LEYLKTKKNKMGHILNEI"
     gene            76867..77643
                     /locus_tag="PLB65_01560"
     CDS             76867..77643
                     /locus_tag="PLB65_01560"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HOQ77242.1"
                     /translation="MKFKRFCLIIGLILQVVLLFSIDIPEPVGFVNDFANLLSEETRN
                     KINDWAIELREKTDVDFVIVTLPDIGEMNEVDYGVKLYEKWKIGSKHDEGVLVLLALK
                     ERKLRFEVGYGSEGYLTDAYINQVYTTMKSYLSKGNENWDEAFIQGSLMLLSTIAKEK
                     GVTLTGLSDYAKRKNTSSSGGLGIFAFLVIFVILIIVTRGRILEWLLWFSIFGGRGGG
                     SFGGKGGNSWGSGNGGFGGFGGFGGFGGGRSGGGGGGGGF"
     gene            77644..78222
                     /locus_tag="PLB65_01565"
     CDS             77644..78222
                     /locus_tag="PLB65_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77243.1"
                     /translation="MIFLKSLTFIVWNVILGCILLFMLKWLLFNRKARYIFGIRIPLT
                     PGFLVAKRDWLFDKVRDLVQDYLDQAANPYYKDGYLSKWIKQIRHFIWDKTDFVDGWF
                     FIPVKFKLVIRDKIADAFTAVVEKFLRKTVPRIVEHLQIEHQIDEFDIQLSVDFFYSY
                     FKKYVYKPLLIGFAALNLVIGIMNMIWFLIIV"
     gene            78226..79398
                     /locus_tag="PLB65_01570"
     CDS             78226..79398
                     /locus_tag="PLB65_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HOQ77244.1"
                     /translation="MNFLFAHFRKLLYRHNIRGDDQKLLLRFLLICLFIAILLLVSSC
                     LAWIFEHHGAEGNSIQSFWDGIWWAIVTIATVGYGDKFPVTFPGRLVGIILIIVGYSS
                     LSFFTGMVASLFVENRLKGAKGLKQIKTHNHIVICGWNKTAEFFLKALVEKEVTDLDI
                     CIVVNDTPDFFERLESCYPALSLKFVRGESIQEETLKRASVETAAQIIIIADEQLDRS
                     SVDDHSIIVANAVRYLAKKDKITVQLINPENRSLLERNGIQNIIVWDDIGGYILANNV
                     TDTNYLTVICQLAQDKNNHIITQRIPTEFVSKSFGELSDYYYKECGYLLLGLFTKEPE
                     LELSSIFTEDNSGIDQFIQYALAKSQKIIREEKNNVRWKPNRDIELQTNDYAILMV"
     gene            79411..79911
                     /locus_tag="PLB65_01575"
     CDS             79411..79911
                     /locus_tag="PLB65_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOQ77245.1"
                     /translation="MIDLDTLSKVPLFNNITIEALTYLQPCFKQMLVPKGTVIIKENT
                     EGDQIYVLVKGCVQVTKDLVKGFDTDQFWTEKVLAKLEGDSFPTFGENGVLGNGPRTA
                     NIIATTDCELYTLTKTDFDSFANYDSKAAYIVMKNIAMKLSESLKLTDDNLVKLATAL
                     YIAVQH"
     gene            80053..>81929
                     /gene="lon"
                     /locus_tag="PLB65_01580"
     CDS             80053..>81929
                     /gene="lon"
                     /locus_tag="PLB65_01580"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOQ77246.1"
                     /translation="MDFTTNKIPRTLPVLHMSNVVMFPYLLMPLVVSDEESKLVIDYA
                     LANDKLMAFFLDEEKDDTGEVELARVGTVVTILRMLRNQDGSISMLLQGSTRVKLQKI
                     VQKTPYFMVDVEAIPEQMDEDTEIQAYRTVAIELLEKIAQESNILNREMIAGLNNIKQ
                     AGRVADIIAGNIDLPIKDRQIILETFDLKQRFRHLNNCLAELIKQMKVENHIRSNIQL
                     EMNEDQRRYYLREQLDAIRRELGETDEVSKEILRWRELIAQKNLPDYVEEVALEELDR
                     LSTMQPASSEYSVIRNYLDWIVNLPWKVYSKDRLNLIKIESILEKDHYGLKEAKERIL
                     EFIAVKKLKGNLKGPILCFVGPPGTGKTSIGRSVARALSRKFIRMSLGGIHDEAEIRG
                     HRRTYIGAMPGKIIMEIKRQGTANPVFMLDEIDKLGRDFRGDPASALLEVLDPEQNNS
                     FVDNYINLPFDLSEVLFITTANSLDTIPPALRDRMEIIEFTSYLENDKIEIARHFLIP
                     REKENNGLAKEKITFSKSALQEIIRYYVREAGVRNLQRRIGSIFRKIAKEVAMGTKQK
                     WTIKADDIAKYLGPRKVTLELANRKPEIGVATGLAWTGFGGEILFCETLRMPGKGNII
                     LT"
BASE COUNT        26409 a        14128 c        14732 g        26660 t
ORIGIN      
        1 tctgcctgtc acgcagaagg ccgcgagttc gagtctcgtc gctcccgcca ttttaaatta
       61 ggatggcaaa tcttaatttg ctataacata taaattatga ttttactttc aatttttaat
      121 attatcctcg aggttgttta cttccttttt tttccgtgta tctatcttct attaaaacat
      181 aaaaattatg tggaagctat taaagccaac ggtataaaca tacaaaaagg gatcttgttt
      241 cattctgctt ctatgggtga attaactgcc ataaaatcac ttataaaaaa acacctgcag
      301 gaatatcctg atattaaact ttgtataact tcaagcactg tatccagttg ccttgaagct
      361 aataacatta gccccaaagt taaaggtttt ctttcagtta tcgatatacc gcatctacga
      421 aaccgtcagt taaaaaaaat caaccctggc ttaatctgtg ttgtggaaac agagatttgg
      481 ccaaatatgt tgctatgggc aaagagaaat aaagttcctg ttttgttttt aaatgccaga
      541 atgtccaaaa gcaccttacg tggatactcc ttgattaagt ttttgtttaa acatctgcaa
      601 gcaccaataa ctgaaattca tacccagagc gaagaagatg caaaacgctt tcgtgaaata
      661 ttcaaaaaac ctgtacttgt tagtggtaac cttaaattct ctatcgaatt gaaggactat
      721 gatgccgaac agttaaaaaa agaatggaga tataataatg acgattttat tatctgttgg
      781 ggctcttccc gtcccggaga agaagaattg cttatttcta tcttgcctta tctaaaacaa
      841 tatatctcta atttacactt aataatcgct atccgacatc ccagacgttt agatgaagtt
      901 ttggaactat tgaatggctt agcatatagt tgctatacta aaagagaaat ttacgatcca
      961 gcagaagata tacttatcat cgataccttg ggcattttgg ataaagcata ttgtatatgc
     1021 gatatagcta ttgtgggtgg ttctttttat gattttggag gccataatcc gcttgaacct
     1081 gcttactata aaaaaccagt aattattggt ccctttcatt attcctgcct tgattccgtt
     1141 aaaaaacttc agcaggaggg tggaatttta ataagcgata aaaatagctt acattctgat
     1201 attataactt tatacaaaga taatgagtta cggtataata tgggggaaaa agcaaaaaag
     1261 gtcttgactc aaaatgctcg cactttagat atttacctta aggccttaga aaaatggctg
     1321 aaggagatat aatgagaaaa gcaatgtatt atactaatga aggcgaaaaa ataaaatgtc
     1381 tgctttgccc cagggagtgt atattaagtg aaggccaaaa aggattctgt agaagcagag
     1441 aagttatcaa tggctctttt gttgccactg catatggaaa aacaacctct ctcgctttag
     1501 accctatcga aaaaaaacct ctttatcatt ttcatccagg tagtatgatc gtatctttag
     1561 gaccaaactc ctgtaattta acctgtaaat tctgtcagaa ttgggaaatc agtcaacaag
     1621 attgcccaac tcgttatttg gcaattgaag acttaattga gataattaaa aaccaacaag
     1681 atcagaaagt tgcttttact tatactgagc ccttgatgtg gtatgagtat attctcgatt
     1741 ttgcggcaaa agctcctgaa atagatattg ttttagtaac caatgcctac ataaataaag
     1801 agccctggag taatatgatg caatatgtaa aagcagtcaa tatcgatata aagtctttta
     1861 gagatgattt ttatagaaat atatgtggag gtacacttga ggttgtatta tctaacatca
     1921 ttatagccaa agaaatgggt gttcatttag aactaaccaa tctgcttatt cctggatata
     1981 ataatagtga cgaagaactt actaatttat ctcaatttat cgcttctgtt gacaagaata
     2041 ttcctttaca cctctctgca tattatcctt gttataaaat gacggtgaga cctaccacaa
     2101 gagaagaagt tgaacacgct tgcgagattg cgtctaaata tttaacttat gtgtatgcag
     2161 gtaatgttta ttcttctcga tattccagaa gttagatgaa aaaagactca atcttaaatg
     2221 accttaggcc tgctgatctg gtactaattc taattgtttt attgacgatt gttttttcga
     2281 tcagctatta tttaagtagt aaacccaatt cccaagtata tatatacaag gataattggc
     2341 ttgttggagt atgttcttta aagaatgata aaataatcaa gattgatagc cacaatatcg
     2401 tggaaataaa aaaaggtaaa gttagaatga aatatgccga ttgtcctgat aaacgttgcg
     2461 ttaagcaagg tttcactaat tctatgccga ttatttgttt acccaataaa gtgataatag
     2521 aaatacgcaa taacggtgaa caaaaaaagt tgattcttca ttaaatataa tatgttgcga
     2581 tttgctttat atgtttctaa tcatggtttt ggccattcta cccgaataag cgctttagct
     2641 gaagaattta taaagtatgg tattttttgt catataatta gcacaaagcc tgcctatctt
     2701 tttcaaaatc tcgatccaca ttattttatt atacatcaac gttctgtgga tgttggagtg
     2761 aaacataatg aaaatctaac tgtggatatg gaaaggaccg tttcttcttt attggaatta
     2821 ctttctaagc gtaacgaaat tatagcaaga gaagttaaat tcttacatca agaaaagatt
     2881 gattgtgtaa ttgctgatgt cccttttttg gttagtgagt ttggagcata ttgtcagctc
     2941 ccggtttatg cagttaccaa ttttgaatgg cattatatat atagctcttt actaactaat
     3001 aacaacataa taaacagcca gactgaattt actaaccctg taactttgag acctgtatta
     3061 aatcttattt ggtctttgta tcaacgcttt gatgcttgtt ttcagcttcc tttcagcagt
     3121 tctgaaagta tatctgcttt taagaataac attccttgtg gtttgctttc ccgtaaaaaa
     3181 gattcttata gtgatatccg gcttataaat aattgggata aggatgtact tatattactg
     3241 gttatctttg gaggggaagg taaattagaa ttggattatg aagctctgtg ttctgctttc
     3301 aaaggtgtgg tcatttctac tcagacaggt gtacaggcaa aaaaccatta tcaggtaact
     3361 atggatgctg actttttgga tttaatatac aatgcggatt ttgttctttg caaacctggt
     3421 tatagtacct tagcagaagc tgtgcaaatg aacaaattta tcatctactg cccccgtaag
     3481 aagtatcctg aagaaacagt gttaatacaa ggtttaagta aatattccaa ctgtttgcag
     3541 atagatacat tgcaaatgaa tactaagcag tggaaagcaa ttttcgaaag cattcatatt
     3601 cggaaacaac ctgttgatca atataaaaat aacaatatca atgtagctgg atcaatacta
     3661 aaaaaatatg cacaaatgat aggcaataac cttgtcagtg tgtttgatct cggttccaat
     3721 aatttgaatt atttactata ttcccttgat aataagcaaa ttatccacaa aacgcaattg
     3781 acgacatccc tggggaaaga ttttaaaaat gataagctta gtgcaaagcg gctgcagagc
     3841 gtaaaacaga ttatagaacc attattgcaa tttgatggat atatcgaatc caaaaaagtg
     3901 cttttagcaa caggagttat gcgaaaggca ttgaatgcga atttgctaac tgattggatt
     3961 gagaagcatt atgaaataaa aagtaaaatt atctcccagg aagaagaaat acgctatgta
     4021 tattatgcag ccagacattt tcagagtggg gaagaggcat atcttgcaat cgatataggt
     4081 ggcttttcta cggagtttat cgatctagga aatgatgaaa aaatacaggg tatcggtcta
     4141 ccttttggtt taatgacatt gttaaacgaa tttgatgtca attgtgatac ggctacaaaa
     4201 tatattcaag atcagcttgc tactttaacc tataatggtt cttatcacct tataggagta
     4261 ggactaactt atacctatct agcagcagtg atttttaaat ttcactataa tgatatagat
     4321 aaaatggaag gtaaaacaat tactaggtct ttattgcagc aactttttca ttcaatagaa
     4381 gttaaagatg aagaccgata tttaccattt ttgctggaag aaagctatct tcctgtgctg
     4441 aagttaagta tacttttcat tatttcttta ctggacagat ttaatacctc cgaaataatt
     4501 gtatgcaatt caggaatagc agttggctac gctaattggc ttgctaaaac aaaaaataag
     4561 gcgctaaaat gaaatttttt attgaaacat acggttgtca gatgaatatt gctgatagtg
     4621 aattaatcgc ttccatttta attgaagccg gtcatcagga agttgaacaa atagaaaaag
     4681 ctgatttact tatattcaat acatgcagtg ttcgcgcaca tgctgaaaac cgtgttttag
     4741 gtagaattca aagcgaaaaa tctcgtaaaa agactaatcc atctttaaag attggtgttg
     4801 ttggttgtat ggcacaacgc ataggtaaag aactaattga agaaaataca gatatcgatt
     4861 ttgtattggg agtcgatcag taccaaaatc ttccagagat tattcaagaa attgataata
     4921 aacaatactt gcttgattta gactctcacc aaatttacaa aggtattcag ccatcatatc
     4981 atagtgatta ttgtgcctat atcactatta tgaggggttg taataatttt tgttcctatt
     5041 gcattgttcc ttatgttaga ggccgtgaac gaagccgtcc ttggcaggat atagtacaag
     5101 aaacgatctt tgcaggtaaa caaggtagaa aggatgttac tctattagga caaaatgtaa
     5161 attcctattt tcattccgat gtgaattttc caagtttgct aaggaagctg aatgaagtgg
     5221 atgaaattta tagattaaga tttatcactt cgcatcctaa agatctatct gatgaattga
     5281 ttgaagttat ggctatttct gctaaagtgt gcgaacacat acatttacct atacaaagtg
     5341 gtaataataa aattttacag gcaatgaacc gaaaatatac tgttgaacac tatctggagc
     5401 taataaataa actgcgtaat tacatcccta atattgcaat aactactgat attatgacag
     5461 gttttcccgg agaaactgaa agtgatttta gacaaactct taaagtactg gaagaaatcg
     5521 gttttgatga tgcattctgc tataaattca gtcctcgctc aggaactgtg gcagctaatt
     5581 taccacatca aatctcggaa caagaacgat tggaacggct acaaagaatg atagatcttc
     5641 aacggaatat tacactgaaa aagaacagaa atcaaatcgg acagacagta gagatatatg
     5701 tagagagttt cagtaaaaaa agcacggata aagtttgtgg aaagacaagg gattttaaga
     5761 tggcagtttt atcaggcaca caaaaggatt ttggtacact acaaaaagta gaggttaaag
     5821 atgcaacagc cagcacactt atctgttttt agtcgcctac ataactgttg tgttaaggca
     5881 tattatgtaa acttatatag gaggtgctta tctattatgc cttataacaa cttattccct
     5941 actcttgctt atagatatca gcttttaaca aggagataat aatgaaaagt atgactggtt
     6001 ttggagatgc tcatatttct aaagatggaa ttgatctgga tgttgaaatc aaatcaataa
     6061 atggacgttt tttagattta cgatttatcc tacctagaga acttagtttt ttcgagtaca
     6121 aaatacgtaa acacataagc aataacatat cgcgtggaac tgtagaaata cggattaatt
     6181 ataatgacca cagagagcca aagctggttt tagataaaat taagttatta aagtataatg
     6241 aattggcaat acaggcttct aatctcttat ccagcgagga agttatttct attgaatttt
     6301 tgctgcaaga accaggagtg attatgagtg agaaccattt agaggatgat ccgattttag
     6361 caaatattct caatgaatgc ttagttcttt ccttgcagaa aattacggaa tcgatgcata
     6421 aggaaggaga gcaaataaag agcgtattac aagattctat gttgaaaatt aagaatatag
     6481 tggcaagtat ttatgaatta tgtgaacctt ttaagaaagc aatgtttgaa aatatgcata
     6541 agcggatttt agagctatta gaaagttatc aggtagaaaa tatggagcaa cgtctaatgc
     6601 aagaagtagc aatttatgtg gataaatacg atgttgctga agagttaacc cggttaattg
     6661 ctcatataaa caccttccaa aatacacttc aggaagaagg agatattggt aaaacgctaa
     6721 attttataat tcaagaaatg caacgtgaag caaatacttt aggttcaaaa ttctctactc
     6781 ctcaaagttt tcctttgatt ttagccatta aagaagaaat cgaaaagtgc agggagattg
     6841 tgcaaaatgt tgcctgaacc cttgctcaaa tggaaatcct ggccttttgt agaacgtccc
     6901 tggacttcta taattttgat agtattttta attttgcttt cgcttttgtt gtatagaatt
     6961 actgttatcg gttggcaaca accgttttac tattttttag gtatgattct agtttttggt
     7021 aaccttctcc cctattttat tattactgaa tatgaacttt atgaggataa ggttcaagta
     7081 cgatatctct ttattaaagt tagcagaccg tacacagatt ttggttgttt ttatgaggat
     7141 aaacatggaa ttatgttatc tacttttaaa atgccaagac ggttagaccc gtttagaggt
     7201 ttatctttaa ggttttcttc cgataaaaaa gaaagggaga aattagtgga aatcttgatg
     7261 caaaagatag ggaagaaata ttaatgaata caataaatag caaggagtta aggcaagcaa
     7321 tattagatta tttctcgaaa tatccacata ctggtttgtc ttataatgaa ttagttaata
     7381 cttttcaact tactcgaact ggaaaatcac ttttaagcgg tgttcttaat aaacttatag
     7441 aagaagggat attagttaaa gataaaagaa aataccactt gaaaactata aaaacgattg
     7501 aaaagccctc ccccactctc aatcccaagt tattacaagg tatttttgat gctacccctc
     7561 tttctcgtaa tttttcttat gcttttgtta gaactccgga aaaggattat tttatagatg
     7621 ctgaagatac gctcaatgct tttcacaacg atatagtagc ttttgaacca aaatacagaa
     7681 agggtcatca ggattatgga attatccaca aaatcgtaaa aagagcttcc gatcatttag
     7741 ccggtgacct aactaaaatg aaaagcagtt ggacatttgt ttgtagtaat ccaaaagtac
     7801 ataagtggtt ctatgtttca gatactgcag gtgcagaaga tggagataag gtaattttgc
     7861 aagtaaccaa ttggggtaat ccccaaactg gtaaattacc tgtaggcagg gttatagaga
     7921 ttttaggaaa atctggtgat ccagaagttg aacttgtagc cattatccgt caatataatt
     7981 taccgttaga gttccctgaa gaagttaccg cagctgcaaa tttactttct gcagatataa
     8041 aaccggaaga ttatcataat cgcagaaatt tgacagattt atacactttt actatcgatc
     8101 cggcatctgc caaagatttt gatgatgcaa tttctataga gaaaactaat aaaggttggc
     8161 gtctttatgt tcatattgcg gatgtagcac attatttacc tgtagaggga gcgatttttc
     8221 aggaagccgc tatacgggga aacagttttt atttccctaa aatagtgatt cctatgttac
     8281 ctgaacgcct ctccactctt atatgcagtt tgcgtcctca ggaggaaaag ctttgtttaa
     8341 ccgttcagac agaatttgat cgtaaaggaa atatccttga gcaacatatc tacgaaagtg
     8401 tgatatgcag taaagcccgt ttgaattatg acgaagtgga taatcttttt aaaggtaaag
     8461 atacagactt gcctcaagaa ttaatagaaa ttcttttaga agggcgagaa ctttcccgtc
     8521 tattaactga aaaaagaatg gaagctggtt atatcttttt tgatttaccg gacattgaat
     8581 atgaatatga tgagagtggt tttgttaggc atttaacttt agctgaagaa actgagagcc
     8641 acaaattaat cgagaatttt atgctgattg ccaatgaata tgtagcaaaa catctatccc
     8701 aggttgctcc aaccacatta tatcgcattc atgaagatcc ggatttcaac aaattagaaa
     8761 gattaataga gcttctttca tattatgggg taaagtgggt tatatatgaa aatctgaaca
     8821 aatcgttgca gtatttgtta aattcttttc ctaatcctgc ctatcataaa gtttttgatc
     8881 gcatcgcact tagaagtatg aaaaaagcta aatacagtat tgaacatatt catcattttg
     8941 gtttggcgat ggattattat actcatttta caagtccaat tagaagaatt tgtgatttag
     9001 ttatccatca tttgtgtaaa acatatttat gccatagcag taatgaaaaa tttactttgc
     9061 cacagctaaa gcactatgcc gaagttgctt ccgagcaaga acttcaagca gaccaatccg
     9121 aacgggatat agaaagagtt tacagccgag catatatgaa aaagaaagaa ggcaatatat
     9181 ataacgggat gatcatatcc gttaattccc gtggcttaat tgtccaatta gaagaaattc
     9241 cgattacagg agttatttta gaaattaatc ttgaagaagg tgattgggaa tatttggaac
     9301 gggaaatgcg ttatataaac cttagaaccc gaaaatttta ccagttaatg gatactttaa
     9361 aagttacagt tggatatgtc gatgatgatg tttactttat tcctttgcct gatacactac
     9421 agcataatgt gcctaaaaat atgatatcca gtgctacaat aaactcttta cagagaaata
     9481 aatcagcagt gcacaaaata tcgtcttctt caaactccta ttcaaaaaat aaaacatcca
     9541 gaaggccgat atcacatcgg tcgcactcaa acaataacca aaattcaaga ggaaacagat
     9601 gaaaaaagca attggcattt ttgattcagg agtaggtggt ttaaccgttt ataaagctat
     9661 ccgtaatgct tttcccagtg aagatttaat ctattttgga gatactgcca gagtacctta
     9721 tggccctaaa tctcctaata ctatcattga atattcatta caaaatgctc gttttttatt
     9781 acagcaaggt ataaaaattt taatagttgc ctgtaatact tcttctgctg tagctcttcc
     9841 ggctatgcag acaataacag acatacctat tatcggagta attgaacctg gtgcagaaca
     9901 agcagctaaa accaccaaaa acaagcgtat tggtattatt ggaacggaag ggactatcaa
     9961 tagcgaggcc tatccaaatg ctataaaaaa acttattcct gaagcagtca ttttttctgt
    10021 tgcttgtccg ctttttgtcc ctttggtaga agagggctgg caagatcatc ttgtaactga
    10081 actggtcgta aaagaatatc ttacgaactt aaaaatgcaa gccattgata ctcttgtttt
    10141 aggttgcact cactacccct tgctctctaa agctattcaa caaattatgg ggagtgaagt
    10201 aactttagta gatagcgcaa tggctattac tgagtatctt gctcgttata tagggtcaga
    10261 aagcgacaaa caacaaggta gcagtacttt ttatgttagt gataacgaag aaaaatttac
    10321 ccaaattgct gaacgcatct taaatgataa tatcaaaaaa ttaatcagag ttcgacttta
    10381 tgaaagctgg tttcagaatt aaaatactat aatgaataag gaattattat aatgtcgttc
    10441 accactacat tcgctgcttt aactctacat cagcaatgct tctgattaca gcatttaata
    10501 gttttttgct atcgatctaa ttgctgatca atctattaga ttgcataatg tgataatata
    10561 aaataaatat agagaggtaa tattatggaa cccttaattt taactgctgc aataacggga
    10621 gctgaaacaa cccgttctga tcaacccaat ttacctataa ctcctgaaga acaagcaaag
    10681 gaagcaaagg cctgttttga agcaggagca agagttattc acttacatat tcgtgaggat
    10741 gatggaagtc cttctcaacg tttggaaaga tttcaagaag ctatttctgc tattcgtaag
    10801 gccgttccgg aaatcataat ccaaattagt acgggaggag ctgttgggga agcatttgaa
    10861 aaacgccttg cccctcttgc tttaaaacca gaaatgggta ctcttaatgc tggaacttta
    10921 aattttggaa atgatgtttt tatcaatcat cctgccgata ttattcgctt ggcagaagcg
    10981 tttaaagaat atggtgtagt tccagaagtt gaagtatatg aatcaggaat gattgattct
    11041 gttgcccgat tgattaaaaa agggattata actcacagcc cgttgcatgt tcaatttgtt
    11101 cttggtgttc ctggaggaat gagtgggaag cctaaaaacc taatgtatat gattgatcat
    11161 ttacatgaag aaattccaac agcaacctgg gctgtagctg gaattggtaa atggcatctt
    11221 ccaacttctt tgatagcgct ggttacaggt ggccacattc gctgcggttt tgaagataat
    11281 attttctatc acaagggagt tattgctgat tctaatgcac aattggtagc tcgtttagct
    11341 agaatagcag aagaaattgg acgccctctt gctactccag aacaggcacg tcaaatattt
    11401 gccttacata aataagagga gcgagaatgc tattaacgga aaatgcactg aaagttctgg
    11461 aaaaacgcta tttccgcaaa gatgaaaatg gcattataat tgaggattgg tcaaaaatgc
    11521 taactcgagt agcatttaat attgctgctg gggacgagga aaaagctaaa cgctttttta
    11581 ctttgatgga ttctggttat tttttaccaa attcaccaac tttaatgaat gctggaggtg
    11641 acttacaaca gctttctgct tgttttgtac ttcctatcga agatagtatg gaaagtatct
    11701 ttgaaactgt aaaaaatgct gctctaattc ataaaagtgg gggtggaaca ggattttcat
    11761 ttagtaggtt aagagaagct aatgccagag ttcgttctac caacggtgtc tccagtggcc
    11821 ccctttcctt tttaaaagtt tttaatgcgg caaccgatgc tgttaaacaa ggtgggacaa
    11881 gaagaggggc aaatatggca attttaaatg ttgatcatcc ccagattctg gactttatca
    11941 cctgcaaaga aaatccagcg gaacttacta actttaatat cagtgttggc ataacagaag
    12001 aatttatgca ggccgttgct aatgataatg aatataatct tatttctcct catacgggaa
    12061 aagtaaataa aaagttgaaa gctcgagatg tcttttcctt gattgtgcag atggctcata
    12121 aaaacggtga accaggaatc gtttttttgg atagaataaa ctatgcgaac cccactccgg
    12181 aattggggaa aattgaatct acaaatcctt gtggagaaca accattactt ccttatgaag
    12241 catgtaatct aggttcgatc aatttagctt tgctgaataa agatggtgat atagattggg
    12301 ataaattaaa agaagtagta tatgatagcg tagattttct ggatgcagta attgatgttt
    12361 ccagatatcc tttgccacaa attgatgcca tgagtaaagc caatcgaaaa attggactgg
    12421 gagtaatggg gtgggctgat ttacttttcc agcttaaaat tccgtatgct tccgaaaaag
    12481 cagttgatct tggagctaaa cttatggaat ttatagatta ttatgccaaa gaacggtctg
    12541 ttgagcttgc aaaagtaaga ggtcattttc ccaattttac caaaagcatt tacagtcaag
    12601 gaagcttgaa aagagagaaa aacagccaag attgggataa tctgaaagag aaaataaaaa
    12661 aagaaggaat ccgcaatgcc actcttacta ctattgctcc tacgggaacg attagtatga
    12721 ttgcagatac ttccagtgga attgaacctc aattttcgct tgcttatatt aaaaatgtaa
    12781 tggatggaga aaaattaatc tatgtaaata aatacttcca gaaggccttg gaagagagag
    12841 ggatatttag taaagaatta gtagaagaaa tattagcttc tggttctctt agtggcattg
    12901 atgtaatccc acaagacatc aagacaatat ttcaaactgc ccatgagatt agccccgaat
    12961 ggcatatcag gatgcaggca gcttttcaaa aatatacaga taatgctgtt agtaaaacaa
    13021 ttaatttttc caattcagcc acggtggaag atatacgctt aagctatgaa cttgcctatc
    13081 aactaggatg taaaggagtt actgtatacc gagatggcag cagagataat caggttctct
    13141 atttaggaac tcttgctact atagaagaag gaaaagaaaa aaaagttgct cctcgcacca
    13201 gacctgaaat aactcaaggt ataactcgtc gtatggaaac tggttgtggt catatgtatg
    13261 ttactataaa ttctgattct caaggtgctt gtgaagtttt tattcaaatg ggaaaagtag
    13321 ggggttgtgc ctctgcccag ttagaagcta ttgcacgtct tgtttctttg gctttacgct
    13381 cacaaattaa gattgaacct ataatccgtc aactgaaagg gattcgctgt caatccccga
    13441 tgtggaataa agggaaaatg atcacttctt gtggcgatgc agtaggtcag gcattggagc
    13501 aatttcttac tctcttttca aatggcgata ttaattccgt tgattttcag gtagaagggg
    13561 ataaatcgga ttcggataga cctaaaggaa atggttttgc gctttgtcct gactgtggga
    13621 atactatcga acatctggag gggtgtttaa tatgtccttc ttgtgtttga gtaaatgtta
    13681 gccagtgctt gacaaaagca gcccttctga ttttttgcct cttacagaaa tagcagatga
    13741 tacatttatc tgctatttcc cttaaaagtg attcagaaat taaataaaag gagtatgaat
    13801 gcgttgtaaa tattgtaatg ctcgccttgc cattcatgat atatggtgta tgcaatgcgg
    13861 aagacataca tctgctttaa aaaacgagct cttctcaata aaatccttat ctgagacata
    13921 tagagcgtat aaagttaata tgtcctcttc cctaccggga gctgctgttg ctgttatttt
    13981 aggtttgata ccggtagcag ttttgatatg gatgttcagc agtattatcg tattggaaag
    14041 caacactact ttgcaacttc tattaaacct acttattaaa tcaatagtgt tttccatctt
    14101 tatacctttt atgttaattg cttttaatcc tatttgtaaa cacaacgatt atcatctcaa
    14161 tctttctgaa atgtttactg ctttacgttg ttatcctcgt tattttttat ggactttgat
    14221 caatgcagta tttttctgca tagtatatat aatatgtttt ggcttgccta atttcagctc
    14281 agctcccatt ttacatttgg tctggcttat tttggttaac tactggattg ccatcgtgtt
    14341 accctctttg gtgttgatgg aggaattatc tatcaacccc tggaaggcaa taattaaatc
    14401 ctaccagcat tttcaggatg tccgttggaa tatctattta cttgctttag tgttagctgt
    14461 attgaatgcc cttggttgtc tttttttctg gttactgtta attcctgagg ccttgatcct
    14521 gcctctatca ttttttgcag tgagggatta tacacgtcgg ctaatagaat ttgaattgtt
    14581 aacctacaga ttgtgaggtg ttattaatga atcgtagaga aatcatttcc ctaataatct
    14641 tgattgtcgt aattggtttt ggagcatata aatatcttac gcgctctttt actgaactta
    14701 aaagtcaata cctgatggat acgatagtag aaatatctgc cacttcgcag agtaagaatg
    14761 ttggccgtca gattgattca gtattcaatt ttattcgttc tctggaagat aaactcaatg
    14821 aatatgatcc aaacagctat ttgggaaaaa taaatgctga ttctcttaat actgtttttg
    14881 atatggatcc tgatatgtat aatttactta ctatagctga tagcttattc aaaatgacga
    14941 acggcgcttt tgatccaact ataaaaccag tttgggattt atggaatttt aatgcagaaa
    15001 atcctgtccc tcccgattct ctagtactga aagaaatgct tagcaaagtt gattttacta
    15061 agatacgata tgatgaaaaa agactgataa agccactggg aatgcagtta acttttggag
    15121 ctattgcaaa aggttacatt ctagataagg cacgcgatca tatgcagacg ctaaatttag
    15181 ataacggata tattaattgt cgcagttcta tgaccttttt tggttataag aaacctcaaa
    15241 ttgtttatat tcagcaccct cgtaaactgg atgattatat tgccagtttc aaagtgctaa
    15301 atcaaagcat atctacttct ggtgattatc agcaatttta cgaatataat ggtgttcgct
    15361 atcaccatat tataagtcct ttcaccggtt atcctgttcc cgatgtgcat tctgtaactg
    15421 ttctttgttc ttctgcagct tggtctgatg gaatttcaac agctcttttt ttgttgcctc
    15481 cggaaacggt tcttgattca attaagcaaa tagcagattg tgaagcaatt atttattaca
    15541 gcaaagccga ttctctggca tcgatcaaaa ctgcagggat gaaggacaaa gatttaaaag
    15601 agaaattatg agcattcccg atattcaatc ccaacctgac acccgtaaaa taactataga
    15661 taaagttggt gttaaaggta tccgctatcc gattgttgtt caggatagag aaaatggcat
    15721 tcagcatagt gttgccgatt tagatatcta tgtagagcta cctcatcaga ggcgtggaac
    15781 acatatgagc cgttttctgg aagttctaaa tcgctatcat actgaagtct ttatagataa
    15841 acttgattca tttttaaacg aattaaaaag cactttaaaa gctgatactg cctacataga
    15901 tatcagcttc ccgtttttta taaaaaaaca ggcaccggtt tccaaggtag tttctctttt
    15961 gggttatgat tgctttttca atgcatcata taacaataat tttgaactgt ggataggtgt
    16021 aaaagttcca gtaactactc tttgcccttg ttccaaagca attagtgttt caggagctca
    16081 taaccaaagg tctgaaatat ttatcaaagt tcgctatagg gaatttgtct ggctggaaga
    16141 attgatccaa cttgccgaag agcattccag ctgcgaaatt tttcccctgc taaaacgttc
    16201 tgatgagaag tttgttactg ataaagcata tagtaaccct aaatttgtgg aagatattgt
    16261 gcgcgaaatt actatggaat tgaataaaga taaacgcatc acaggattct atgtggaagc
    16321 tgaaaacttt gaatctattc atgctcacaa tgcttatgct ttgataagta gaatataaca
    16381 tcgttatgaa cgataaatta attactggaa atatttcttt ccccaagcca atatgcagga
    16441 atttatctaa tggcctggaa gttatctccg taaaagataa ttcccatcca gttctttgcc
    16501 tccagcttta tataagaact ggttccacct tagaaaatga tcaacaaaga ggatattctc
    16561 attttataga acacttaagc tttaaatcta ccagtgattt tccaaataac aaaatatcgc
    16621 tttatgcttc gggtttgggt gggatgctca atgctttcac agattacgat tgtacttgtt
    16681 attatttaaa cttaccttca gaaaagctaa aagaaggttt ccatattctt tctcaacttg
    16741 cttttcacag cactttcagt aaagaagatg taagcaaaga aaaagaaatc attctggaag
    16801 aaattaagca gtatcaaaac gatcctgaat cggactttct ggagtatatt caaacaaact
    16861 gctatcaaaa aagccctctc agatatcctg ttttaggtaa tcctaaaagc attaacagcg
    16921 caaggtataa agaacttcgc gaattttata aaaatcgata tactccagtt aattcctttc
    16981 tagtaatctg tggtaactat caagaaaatg aattggatgc ttatcttgat acttattttt
    17041 ctgcttggga aaacataagt aagcctcttc cctatctgtt agatattgaa ccggaaatga
    17101 atggctttcg ctacatcttt aggaaaaagg caattggtga tgatactatc gcctttgctt
    17161 ttccagaact tagtgaaaaa catcctttgg ctaatgcact tttgatagct atgcgttatt
    17221 ttgccatagg aagatcttcg cggctatata atcgcttagt agaaaaagat aaactatgtt
    17281 ccagtgtgaa agtaaattct ctttgtggag ttctttccgg agtttcagtt atttatatca
    17341 ctcccatatc agcaactttt attcctgata ttattaggat agtttatgaa gaatattccg
    17401 ctcttttaca gtttggaatc ccattagcag aacttgaatt aataaaaaaa gatataatta
    17461 atagctggtt atttagcttt gaagggatgg aaaatcttgc caacctgata gctgcagaga
    17521 aatttacagg agatttatgc cgccttcaga aatatggaga tgagattaat tctatttcta
    17581 tcgaagccgt aatggctgct attcataaat attggctacc cgaaggtatt actgtttacc
    17641 atgaaggttc ttgtgaaatt tctggaacaa aaaaacaagc taagcaaata aaagaaagtt
    17701 tagcccccaa aaagacaata ctatgtgcag aggaaattca taatatttct attcaaaatc
    17761 tgaatccccc accattatac aatgcagaaa atacgagaag aggttacata acccaaatag
    17821 atgataatta ttaccagatt gtcctctcta acaatgtaaa ggttattttc aaacagcaaa
    17881 agaataataa cattagcggc ttttccttat caactcctat tagtcagatt agcgagactc
    17941 cttcagaaat tggtcataat ttcttttgta cttctttaca gctctataaa acccaaaaac
    18001 atagccatca agaattgctg caatattctc ggaataatgg ttttaatata aggttaattc
    18061 atcatttaga tacaaccact ttcaggggaa aatgtttaag tagcaatctt gataaagcac
    18121 tagctatgct tgcagaatcg atttttttac caagttttga tcataactat ttctctgtat
    18181 taacatctac tgctttagat ggaatccgtc gtgataacga ttatcctgtt agctatgctt
    18241 atatgaactg gtataaaatg ctggtaggca ataaaagtaa tctatttcga cctacagggt
    18301 ctgcaaccca aattcgttca atccgtataa aacatcttca gcaatggtat gaagattgga
    18361 atataggaaa agacttttgc ttagctatcg taggaaatca taatccccaa gaaattttag
    18421 aactttgtga aaagaacttt ggcttggttc ataatgtttc tcattcgccc aagttcaaac
    18481 cattctattc tccttcttca gtgcatttta aaaagaccta tcgaaatact gatcaagcca
    18541 ttatatatat tggaggattt gcttctcctg ctattgatcg agatgaaaat accgctttct
    18601 atattttatc ccaaatttta ggtggtgata tctcttctcg cttttttgac attctaaggg
    18661 aaaaatacgc ttatgcttat caaactggtt tcgatttcca ttctttgaac gaattaggtt
    18721 tctggaatgc atatgccttt tgtgataaag acgactataa taattgcctt gctttgatga
    18781 aggaaattat ctattctatt gctgataaag gagtaaaaga aaatgaactg gaaactgcaa
    18841 aacaatactt aattgggatg aatcgctttg aagatgagag tgtgtcttac acagcatcaa
    18901 ctatttccaa tcttgttgcc ttgggctatg aaccagaata ttatctctta cgcgaagaac
    18961 gtattaaaaa ggttaatagc gaattaattt ggcaaatagc caaaaagtgg ctattgcctg
    19021 ataaccaata catctatatt atgttgtaac tatgttcaaa cttggtatcg acatcggctc
    19081 tcgtaataca aaagttgtga tctttaatga acacacaaag agtattgagt tcagcgattg
    19141 caaatcaact gaaatattta tccttagcgg agttaataaa cttttacaca atgcattcct
    19201 tgcttcggga attaataaaa atgaactaac tgctatcgga gttacaggtt acggtcggaa
    19261 actttttaaa gatgcaacct taactatatc cgaaataagt tgccataccg ccggttgttt
    19321 atattatttt cctgatgcaa aaacgataat tgatatgggt ggacaggact ccaaaatcat
    19381 cacccttaca gacagcggaa aagtaaaaga ttttgttatg aacgataaat gtgcagctgg
    19441 aacaggacgc tttttggaaa tgactgcaat gcgtttggat tgtgatgttt ctgatctttc
    19501 tgctatagct tccaaatcaa ctaaagaact aaaactaaat tccacttgtg ttgtgtttgc
    19561 cgaatcggaa attatcggaa tgttatcatc ttcaactgct ccagaaaata tagtccgttc
    19621 tgttcatcga agtattgcaa aacgtatttt agctcaaatg gcatctctgg tttgggaacc
    19681 acctattgta tttacaggag gtgtagcttt aaaccaagat atagtaaaat gtttcagtga
    19741 atcactttcc tatcaattaa ttacacctcc cgatccggaa attacagctg ccttaggtgc
    19801 tgctatctta gcataatgaa attcgattat catattcata ctgaagatag ttacgatagc
    19861 tctattaaaa caattgattt gatagatagg gctattcaat taaaatatga tgaaatagct
    19921 attacagaac atctagattt gctacctatt gaaatgaaaa gctatggagc accttccttg
    19981 aaacattatt tcaaaagaat taatgattta caaaatacaa tatcagaact cacattactt
    20041 agtggtgttg aagtaggtga ttatcatata gttaaggatt atgctgtttc acttttagag
    20101 ggtataaatt ttgatttgat attgggatct gtgcactttc tatcggatca aacgaatgta
    20161 gctattcctt taaagaaaca gctttccgaa gaacagatta gagattatta tttgcaaaac
    20221 ttggctttag tatccaattg cgatattgat gtccttgccc atttaggggt ttataaacgt
    20281 tgttataaca gtattccgga tgaacgaatt cactatccga taatacaaga tattttcagg
    20341 gttattatag accgaaaaat agctctggaa atcaattaca gttcattgcg tagaggatat
    20401 ccttctttta ttccggagca gcatatttta gaattatatc tagctttagg aggacaactt
    20461 ttttctttag gtagcgatgc acatttcatt gagcattttg atttgtttag agatagcatt
    20521 ccgcaagaat tcagcaattc gtttcgatat cacttatagt aaacatagat aacatttatc
    20581 aatatcctga atttatcctg ctgtactaaa tgtatactgt gcttcactat atttaatctg
    20641 ctttgctatt ttccattttt tgtactttct ttaggcataa atttcccttg ccaaaaatgc
    20701 tatattgatt atactgtcct aaaaataaac ctgcgatgag agataagttc tggcatcaag
    20761 ggtggtaaaa tcctgtttga tttgcctttt tatcatctct cgctgaagga aattatatta
    20821 tgaacgagag atgtcatatc ctcactatta ttatggaaga ccgtgaatca gctttccatc
    20881 cggtaaatga actcttacat agttatgctc cacatatatt attaagggtt ggatatccta
    20941 tgcgcgattt gggtgttgct gtaatctttc taattgtaga gctaccgatc gctgaaatgg
    21001 gttctttttc tggaaaatta ggacagatta agtccgtgaa agtacaggtt acaactttaa
    21061 agcttgaaaa aggagaaaaa aatgaacatt agcacagaag gcgtaaaatc ctttattcct
    21121 acaaccgaaa ttgaagaaca tctggcaagg aatcgtttcg ctgatacgat tcggataagg
    21181 gatattattg ataaatcatt agataaacag cgtttaaatc cagatgagac ggctgcccta
    21241 attaatgtta ccgatccaga attaaagcaa cagattttag aaggcgcaca tactcttaaa
    21301 gaacgcattt acggtaatcg tatcgttttg tttgctccct tatatgtagg taacgattgt
    21361 ataaacgatt gtgtttattg tggatttcgc agttccaata aagaatgtca tcgagctaca
    21421 cttagtaaag aagaacttat ttccgagacc gaagctttag aagatgcagg gcataagcgt
    21481 ttaattatgg tttatggtga acatccgaaa tattcaccgg agtttattgc agacacagtt
    21541 caaacggttt ataatgttaa acataacaaa ggtgaaattc gcagggtaaa tattaatgct
    21601 gctccccttg atattgaagg ttttagaata gtgaaatccg taggtattgg aacttatcag
    21661 atttttcaag agacctatca tgaagaaacc tataaaactc tacatccaag gggacctaaa
    21721 agtaatttct tatggcgttt ggatagttta gacagagcta tgcaggctgg aattgatgat
    21781 ttaggtattg gtgctttaat gggtttatat gattggcatt ttgaagtgat gggcttatta
    21841 tatcatacta tccatctgga acaaagattt ggagtaggac ctcatacaat atcttttccc
    21901 cgtattgaac ctgctattgg gactaatttt acaaatcaac ctccccatag agtatcgaat
    21961 gaggatttca agcttttgat agccagcctt cgtttaagtg ttccttacac agggatgatt
    22021 cttactgcaa gagagcctat tgaagttaga gatgaagttt tgcgctatgg aatttctcaa
    22081 attgatgcag gaagtagtat tggagtaggc gattatgcgc ataaggatga tgaatctaaa
    22141 cgcaaaagtc aatttgtgtt aggagatact cgttctttgg atgatgttat atacgaactt
    22201 gctaacagta gttatatacc ttccttctgt acttcctgtt accgggcagg aagaaccggg
    22261 gagcatttta tggaatttgc tatccctggt ttcgttaaac gattttgcac tcccaatgcc
    22321 ctacttactt ttgctgaata cctttacgat tatgccagtg agcatacaaa aaaagtcggt
    22381 ttcgacttga ttgatcagga agttgcaaaa atagatgatc cccaaatgca gatatcggta
    22441 aaagataatt tggcaaagat gaaagaagga atcagagacc tacattatta gtattaatag
    22501 cttaagtgga atgggcttaa atatttaacc cgctgtatat atgataaaat gaaaacggag
    22561 cttcttataa ctccgttttt ttttgcatat tgaactcggc aataatttac tatgccacaa
    22621 tctcgaaagg aaacaatgcc attcaagaaa agtttctatt ggggagatca ctacgataga
    22681 tctgatagtt aaccttatcg ctgatgatga ataaatcgtt attatatgtg atatgtaaag
    22741 taggaaaaaa agttaaacca taattataat aaataatctg ttgaccaata gcaagttcta
    22801 cgatggataa atatgttgtt tattatacat aagaaaaagg aaagcaggtg gaagagaatc
    22861 ctcctgctac aaaagttatt ttataaatct ggtccccgtc tttccttcaa aagcatccac
    22921 tattttttct atggaagtaa tcaatacttc tgatcctccg ccttccagaa aatcgattgc
    22981 tgcttgaatt ttaggtccca tactacctgc tggaaattgt ttttcattat agtagtgttt
    23041 tgcttcagcg atggtaattt gatccaatgc ttgttgattt tccttaccat agttgatata
    23101 tactttttct acgccagtta atataacaaa taggtctgct ttaatttcca gagctaaaag
    23161 tgctgatgca aaatccttgt ctattactgc atcaacccct tcataagtgc catcttcttc
    23221 aatatatata ggaattccac ctccaccaac agcgataact atctctcccc tatctaccaa
    23281 ttcattaatt gtggaagctg gtataatttc ttttggataa ggtgaaggaa ccacacgccg
    23341 aaaaccttta ccagaatctt cttttaagat ccagcccaat ctgtttttaa gttcttctgc
    23401 ctgtgcagca gtataatatg tgctccctac aaatttagtc ggatttagca tactaggatc
    23461 atttttatcc actactacct gagatatgat agtgataacc tgtttttgaa ttccactttt
    23521 atgtaatata ttctgtaaac tctgttctat catatagccc atagtgcctt ctgtagcagc
    23581 atttaataca cctaaaggca attgaggaat tccctccatt tctcctaatt cctgttgacg
    23641 gagcagattc cctacttgag gtccattacc atgtgttata cttaaatgat agcccctttt
    23701 gattaattct acaataccac ctaacgattc acgggtattt gaaaattgtt gtgctattgt
    23761 acctttttca ccagctttaa taatagcgtt tccgccaagt gccagaacag cttttttttg
    23821 catttctcaa atccttattg ttctgtataa attattttga ttgaaaaaac aggttaattc
    23881 tcatttgtgg aacagagaat tctccctaat attcatagcg agaagttaat tggaggtgga
    23941 taagtaccca cctccaatgt attatcgatt atttttttgt agatttcttg ccgaagttct
    24001 ttaagatgtc agcaatagta ggaacaccga tttcctgtaa ttcatagctt acttggcagg
    24061 caggtttatt tattttaata attctgcgaa ggtctatcgg agtagcaatt actacagaat
    24121 cacattccgt actgttaatt gtcttttcta agtctttaat ttgcttagaa ctatatccca
    24181 tagcggggag caaagatcca actccgggat atttttcgaa agtctgtttt atttcaccaa
    24241 cagcccatgg acggggatct acaaggtctg cacaaccata tttttctgct gcaacaactc
    24301 ctgctccgta ttgcatttct ccatgggtta aagtaggtcc atcttccact actaaaactt
    24361 ttttgcctaa gatcatttca gggtgatcta caaataaagg agaagcagcc tcaataattt
    24421 gagccgttgg attaattgct ctaacatttt ctcgcacttc attaatatcg tacagattgg
    24481 cgctatcgat cttattaata acgattacat cagccaagta tacatttgtt tcacctggat
    24541 aataagaaat ctcatcacca ggacggtgag gatctacaac tacgatttta atttcattat
    24601 cggaagtgta gaaaggaata tcattatttc caccatccca aacaataaca tccgcttctt
    24661 tctctgcttc ttttaaaata gcagcataat ctacacctgc ataaattaca ctacccatca
    24721 taatatgggg ctcatattct tccatttctt caatagtgca attgtgcttt ttgaggtcat
    24781 caagttctgc aaaacgctga actttttgtt ttacgaggtc accgtaaggc ataggatgac
    24841 gaatagagac aactttcaat cctttagctt tgagagcttt taccactgtt ctggttgttt
    24901 gagactttcc gcaaccagtt cttaccgcgc atacagtaac caaaggtttg ctggttttca
    24961 gggtagtatt atctttgccc atcaacataa aatctgcacc agtggagtta ataattgagg
    25021 ctttatgcat cacaacttcg taaggaagat cgctataagc taaaactact aaatcgacat
    25081 tgtgttgctt aatcaaattt ttaatttcgc tttccggata aatattaata ccatcaggat
    25141 agagttttcc agctaaagca gccggatatt ttttgtcgtc aattccagga atctgagtag
    25201 cagtaaaagc tacaacttca taatccttat tatcgcggaa gaaaacattg aaattgtgaa
    25261 agtcccttcc tgctgcaccc ataatgataa ctttgcgttt cattgatgaa tctcctttat
    25321 ttaatatttg ttacaaattt gctttcaagg tttaaaatag ggttttaccg tcaaggaaaa
    25381 tctatttttt ttgaattact gacatataat atggaaatac caatatgaaa atcgggcttg
    25441 acaaaaaaag gctgtcttaa aaatagaaaa acaaatgtgc tgaagtggtg aaattggtat
    25501 acacgctagt ttcagggact agtgagcaat gagctcatgg gggttcgagt ccccccttca
    25561 gcaccatttt tttgtgcacc gaattatttt ccctcataat tattcgagtt agtatgcgtt
    25621 acccaggata aaataatcat tgactaacaa tagctcatta tataagttgt ttgttgtttc
    25681 ttacattgat acaaaactat ttctttctac tttcgattaa gaaataagct ctgatttttc
    25741 aaagttcgat tggaaagcag tttgcgaaga aattagaaag cattaatata tataaaaagg
    25801 aatgtataga atggaaaaga aaatttttgt cctggataca aatgtactga ttcataatcc
    25861 gcaagctcta ttttcctttc aggataatag agttgtttta cctattgtgg ttattgaaga
    25921 gattgatcaa tttaaaaaag gggttgatga aaaaagtagg aatgcccgtc aaattggtcg
    25981 ttttcttgat gctttacgca aaaaagggag tttgcaggaa ggtgttccta cagagaatgg
    26041 gggaacaata caaataacga tcaacaaaga aataacggcc acagcttccg agcttttatt
    26101 tatggaccgt aatgacaatt tgataatcag tacagccttg tattttaaaa ataagtatcc
    26161 cgaatctatt gttactttgg tttccaaaga tgtaaatgtt cgggtaaaag cagattgtgt
    26221 tggcatcaac gcagagaatt atgaaaccga cactattaaa tatgatgaat ttttcaccgg
    26281 atgggaaata cttgatgtgg aacctgagca ataccgtgaa atagaagaga agggatatat
    26341 aaacaacatc tttggggatt tttttgccaa tcaatttgtt agaatcccta ctcctgataa
    26401 tcctgataaa tatacaattt tgcgttacca taaaggccga aatcaacttt atgtaataaa
    26461 acactatact ggccaatatg tatatggaat caaagcccgc aattttgaac aagaaatggc
    26521 tttagatatt cttttggatg atagcatcaa gctggtgagt ttatctggaa aagcaggaac
    26581 tgggaaaacc cttttggcta tagcagctgg cttgcaaaag gttgtagaag aacaaaagta
    26641 ctcacgcttg gttatttctc gtcccatttc tcctttagga aaagaccttg gctatttacc
    26701 tggttccaaa agtgagaaat ttaatccctg gatgcaaccg atctacgata acatggagat
    26761 actgctatct tcgcatttag ataaaaacaa accgaattcc aatacaaaaa aaagaaccag
    26821 tgttaatgac cttactgatt atggtttttt agaactggaa cctttaacct atatcagagg
    26881 acggagctta cctgatcagt tttttataat tgatgaagct caaaatttga gccctcatga
    26941 aatgaaaacg attattacca gagctggcga aaacactaaa gtagttctaa ctggagatcc
    27001 ttatcagatc gatataccct atcttgattc ccaaagcaat ggactatctg tttccgttga
    27061 aaagtttaag ggtgagatcc ttgtgggtca tatcacttta gataaaggtg aacgaagtgc
    27121 tttagcagat ttggctgcca aatatctata agctatgata aaaaagaata tttgcgatcg
    27181 cagctgggtt gaaattgatc tctctgcttt cagaaagaat ttaagcattc ttaaaagtta
    27241 tcttgaaccg catcagcaat ttatgatgat tgtaaaagca gatgcttatg ggcatggagc
    27301 aattgaaata agtaaaattg cttctgaaga aggagcagtt tattttggag ttgccaatcc
    27361 agaagaaggc cgtttattaa gacttcaggg atgcaaatct ccaattttaa ttctttctcc
    27421 ctgtttaacc agcgaaataa atgttatttt agacaatgat ttggccgtta atgttacttc
    27481 cttgaatttt gccaaagctc taaataaagc tgctgtagta aaagataaac aggcaattat
    27541 ccatttgaaa gtagatacag gtatgcatcg cagcggaatt ctggatagcg aattttctga
    27601 tttttattcc cagatacaaa agctgagcaa ccttaatata gaaggggtat tcagtcattt
    27661 tgcctctgca gatgatgaca tagagtttag taatcagcag gaagaaagat tctggaaagt
    27721 gatagaccaa cttcctgaaa gaccaaaata tattcatata gataattccc atgccctaat
    27781 aaataaattg ggaaaacgaa gtaatttagt acgattaggc attatggctt atggaattca
    27841 gattcctttg caagaagatc tcggtttaca gccagttatg acttttaaaa ctactctctc
    27901 acaaataaag catatctcca agggtgaatc tattggttat aatcgttcct ggatagctga
    27961 taaagattgt gtttatggaa ttctacctat tggatatgca gatggctatg attttatgct
    28021 ttccaataat ggtgttgttt ggctggataa tgtactttgt aaagttattg gccgaatttc
    28081 aatggatatg atatgtatcg atttaagtga tatagataaa cccaaaatcg gagatgtggc
    28141 aactctctta ggtggttcaa gattagaaac aagggcagaa aatttagttg ccaaatatgg
    28201 aggaaattct tatgaattac tttgccaggt gggacgacgc gcaaaaagtt atttttttca
    28261 aaatggaaaa ttgattcaca gcatcccatt attaaggcga gattttgtcc cagacgattt
    28321 tccggattca aaactgaata aaattatcga atcagcaatt gcccagcggt tacagagcat
    28381 agaaattgga gaaattatct atcgtgaaat gttaagagat ttcttttatt ataaggatag
    28441 agatatccat taccgccata atttccatca taaaattgtc ttttctagtt ctttgattgc
    28501 tggttattat aatgcatcta ctttattaaa tttcgacaaa gtgctcagta acgattattt
    28561 tttagttgtt tgtgcatcct ctgatgaagt cttacgaaga tattttaaaa atggagatgt
    28621 agaatacaga tggcttatgg atgatagttt taaacttgat aatgaaagct ttattgtttg
    28681 tctggttcag gtagatgaac tcatcttgga aaccagtatc aagcatataa atgactgttt
    28741 agaaattaaa tgcagccatc ctaagctaag gggaaaaata ggtaaaaaag tgcattttac
    28801 tatcaacacc caaaccttat atccaacctc tgctcatcaa ttcagtgttt ttatcacaga
    28861 actcactagg ggagtagata tcacttttca atatccacag gaaattaaag aagttgattg
    28921 tgttcctttt ttttcaggac aagataaaga tccccttatt actcaaaata atagagaaat
    28981 aagagtgtct acagaattga atgaatggat atttcctatc agtggagttg tttttgccta
    29041 ttgaaacata aaaaaagatt gacaaatctt cctcgtcaga attgtagttt ctccattata
    29101 attatttata tttgttgttg ttatctaacg gtgacttgct tatgaaaaga ttgaacttat
    29161 tattaatgtt aattttgtgc ataccacttt tagctggaag ctttaactgg tattcacaaa
    29221 atgacgaacg ctggaaatcg gaaaggttag ggtctggaaa tagttccata ggtagtagcg
    29281 ggtgtgtgct tagttgttta tctatgcttc taaatgcaga ggcaagtaat cagtatatga
    29341 ctcctgatcg cttaaacgaa tggttacaaa aaaatggtgg ctatgctgga aacaatatgc
    29401 gttggcaaat tccaggtgaa attgatggca gtggattggg gctcgaatta gttgctcaaa
    29461 gcacaaaacg taacgattgg gatttccttt cgggagaatt ggaaaaaggc aataaggtta
    29521 ttgtaaaagt taatggtaga cggagccact gggttcttgt tatcaaacaa aatggcccca
    29581 aagataaccc gtcttcctat ataataaatg atcccggcat caaagaatat gaagaaagga
    29641 cccttgccta ttttggtggc ttcaaatcgg cgcgttctta ttccggtaat tggttagatg
    29701 aacaagcctt cgatttggat agtgaaatta atgtcgttcc tgtacctcaa gatgaactat
    29761 ttctttatga tgttgctaaa ttacctcatc ctgccgatgt ttatgtaact ttacataata
    29821 atttacctgt cgacattact ggatacttta ttcttgggct ttttgatgaa gatggaaact
    29881 acatccgtac tgttgattat gaatatgtta cagtagaagc cagtggcaaa tacgatcttt
    29941 tatatgaact atccgatgta aaacctctaa atgaagaaca ccaagaatta ataatcattt
    30001 atagtaaata cttctctgct atgccttctt taactgaatc ccttgagttg ataagcaaag
    30061 gcataagaaa ctacacaact atcacaaatt aaagcttata actacatatt attatcaagt
    30121 tctataataa gcgaactgat ttttgatttt ttctatacgc acatttctgt taatattttc
    30181 agactaaaat ctaaattatg ttttctgcca ggcaacatcg cataactcgt aaggagtcat
    30241 ataattcaga gctacattta taattagctg aatagccagt tctttggctt tttattacag
    30301 aattgagact aaattcatac ttaagcgtat taaaaaaccc tccattttag tatcatcaga
    30361 gtggtaatac taacctacat tcccttacac tgcaaagcac tcaggtatct tccaggataa
    30421 tacaacaaac tatgatcgga aggatgaata atgcctttag aatttacaaa tttatgtgat
    30481 caaatttgcc gttttcagtg atcacttata ataatcagat tattccctta cttataaccg
    30541 acaatcatat ctttatcact cctttatcaa tccttaatca ctccttaatt attaacgctt
    30601 gattaagggt tgtttaagga ttaatatact tttgatgaga gagtaaaatt aattatctct
    30661 tatcgataag aactatggta taatgtggag agtaagcatt tagctggagt atatttggtt
    30721 ccttctcttt tctttataga caaaaatatg gtttaattta tgtagtaacc cttgttgttt
    30781 tacagtgtat gtaacttata aataaagaac aaaatacgat gaatagaata atgagaatgc
    30841 gctcaattat taatcccgta atatcgatat ccataaaaaa atggacaaat atgaaggttt
    30901 tagggagata gagaaaataa aataatgaat ataaaaaacc gaattttttt tccaatattt
    30961 atccaactta attaaaatat gatcgagata atatctatac ttctggttat actacgatct
    31021 tcaagctatg atgtatatat ctaataacta tctattatgc aattaactaa agaatttgct
    31081 tgacggaaat gagtatatgg aaaaaaatca acacaaaagt atcgcataag ttaaaggagg
    31141 tttaatgaat aactatttta tggaaaccca ccatcacgaa tggattctta ctaaccgtag
    31201 gggtggttat gctttgggta ctggtaatct tatcaatcaa cgtaaatatc atagtttatt
    31261 agtaagtagt gacaataaat ttaatcgtac ccatttagta gctggtattg aagaaaagat
    31321 tgaatggcgt ggtgagatat tcaacttgga tagcaataat tatagtaact gtatatatcc
    31381 tgagggtttt ttacatttgg taaaaccgtg gttgagacca tatcctattt ttttgtattc
    31441 tgctcttccc caccaaaatg atattctaat tttgaaagaa ataatgatgg atgaatctac
    31501 caatacaact ttggttaaat atactaattt agggcatcat ttacttcatt ttgaacttca
    31561 tccaaaattt acgatgatta atcaccacca gctcaattgt cctggaagtt tagatttttt
    31621 acagtttgat acagaaattg aagaatttaa taaatttact cgttatagaa ttcatcgaca
    31681 ggataataat gtttcattat atggtgctgt tttacaagga gaaattattc ccaatcgtaa
    31741 tgtatattac aatgtttttt atccttggga agtaatgaat ggttatgaag gtattggtga
    31801 tcagatcact cttgttcaga ttagttttga tttaaaggta ggcaaaagca attatcttct
    31861 ctttagtgat acttcaatag aagatgcaga gatattaata aaacgaatag aagaacgata
    31921 tgctcatttg cctaaacctt tggattatcc gatatttccc gatagcgatg atgctttgtt
    31981 gaataatttg gattataatg ataattttct ttataactat gatgaatatc tacaaatcct
    32041 cgaatttgct ttaaaggact ttttggctaa tgatgatgtt gtggctggtt atccttttta
    32101 tggtgcttgg ggtagagata cgatgattgt tttaaatgct ttattacata ctcccaatca
    32161 acaagaaact gtagaaaaaa tactgaataa atataaaggg tatattaagg atgggctcat
    32221 acccaatatg atggtagaat ctggtaaaga agcaaattac gatagtatag attctaccct
    32281 atggttaata attctgcttt ggaagcttgg aaaactgaaa caaacagtta aatactggaa
    32341 agataccatt gctgtttcgg aagaagtttt aactaatatt ctacataatg ataaacaccc
    32401 tttttcggtg cgatctgatg gacttattga gttgcattct gattttgctt atggaacttg
    32461 gatggatgtt cggattgagg gaaaacctat aactccccga aatggagctc ccatcgagat
    32521 aaattcactt tggtataatg ctttgtgttg ttatgaatca atgtgttctg cctacataca
    32581 aaaatctcgt aagaagtatc aacccaacga tgaactgata atgattaaga atatggtaaa
    32641 aacatctcta caaaagtttt ataatgaagg ttatcttgca gatagaattg tgggtgagga
    32701 acttattatg gaaatacgac caaatgctat cattgctctt tccttgcctt ggcaggtatt
    32761 ttcgcaagaa gttttgcaac aggtatttga acgaagtttt aatgaactgt ataccaatta
    32821 tggaattagg actctaagcc ctaatgatat ccggttccgc aaaaaatact atggaacaca
    32881 aagagaaaga gaccttgcat atcataatgg aagtgtctgg gcatggttac ttggaccttt
    32941 ttgtgggtta tatgaacgcg cctattctgg cataaaaaca aaagaagaaa ttattgaagc
    33001 actcacttct tttatttcca cttttcgtaa cagttttatg aaaggtcata tagcttccat
    33061 tgcagaagtt tgggatggtg atgctcctca ttttccaaaa ggggctcctg ctcaagcatg
    33121 gagtgttgct gctttatata atgtagagaa ctatattcta tttttggagg aaagcaaatg
    33181 aaaatcttga tgtttacttg ggaatttcct ccccttattt ctggaggatt aggtatggct
    33241 tgttatggaa tggtaaaagc tttacttagt caaggaataa aaattgatct tgttcttccc
    33301 actaaagaac tagtttattt tcctttacgc gatgaaggcg atgcagatac tctacctgct
    33361 gtttttattg aaccagaaaa gcaaatagaa tacacaagac gagcttttac ttgtttaagt
    33421 gaacgtcttg aatatattgg agtaacaaca catccagaaa gttatttcaa tatgagtgaa
    33481 atagcagaat actctaactt aataaaaaaa cactacgaat taagtgaagt tactataagt
    33541 gaagaacttg aactgctgaa agaaatgacc gctcatctca ttggcgatga ggatcttatt
    33601 cgtaaagttc aggaatatac ccttagggca aatcgctttg ctaaaatatt agaatatgat
    33661 ttaattcatg cacacgactg gcttacttat ccgagtggaa ttctgacaaa acaaatatca
    33721 cataaaccct taattgttca tattcatgct actgaatttg atagagctgg gggtcctggt
    33781 gatccacgaa ttcataaaat tgaatatata gggatgactt atgctgataa agttatagct
    33841 gtttcacaat atactgcaca gatgattatg agtcgatata gaattgattc tggtaaaata
    33901 cgcatcattc ataatgcgtt tactttaccc gaaaccaatt taaataacaa gaaacgtata
    33961 tttataggac ctacaattct ttatcttggt agggttacca tacaaaaagg accagattat
    34021 tttttagata tggctgcaaa agttttgaaa gttcatccgg aggcaagatt cattcttgct
    34081 ggaaccggag atatgcaaag acaaatatta cgacgatcgg cttccttgag cttaaaaaac
    34141 cgttttttat ttacagggtt tcttaatcgc aagcaagtag aaagaatttt acaagccgcc
    34201 gatatctatg ttttaccttc tgtatctgag ccctttggaa tatcaccctt agaagctatg
    34261 tcttatggaa taactgctat tatatcaaaa caatctggtg tcgcagaaat tattcatcat
    34321 gcttttaaaa ttgattattg ggatgttgat ctttgggcgg aaacaattaa tcacctaata
    34381 gaaaatcctg aggaatgccg taaaattggt attgaaggta tgaaagaagt gcaacgtatc
    34441 caatgggatg aagcagctga aaaaattaga caactttacg ctacaacttt atcagaattt
    34501 agcgaacagc gtgtagaata aggagctatt gatgttaaat attgttttct actttcaggt
    34561 tcatcaaccc taccgtctta ctcatttcaa tgttcttgat attggtaata atgctaaaat
    34621 atttgatgaa agattgaata gcaatgttat gcgtaaggtg tctaaaaact gttatttgcc
    34681 cactaatcaa ttacttttgg aattgatcca aaaatacgaa ggtaggttta aagttgcatt
    34741 ttctattaca ggaacagcta tagagcaatt taaactttat tctcccgaaa cccttgattc
    34801 ctttaagtgt ttaatagata ctggttgtgt agaattgctg ggggaaacat attttcattc
    34861 gctatctttt cttttcgata ctaatgaatt tctcgaccaa gtttatatgc atagggaact
    34921 tatgcaaaag gaatttggtt attatacaac cacttttcgt aacacagaat taatctatca
    34981 ggatcgcttg tcggatataa tctacgaaat tgaaggtttt aaaactatca ttactgaggg
    35041 agtggataga attttacaat ggcgcagtcc tttgtatgct tataaaaatt acgctaagaa
    35101 cctgaatttg cttctaaagt actatcagct tgctgatgac attgcattcc gattttccaa
    35161 tagagactgg ccagaatatc cgttgactgt tgataaattt gtaaattgga tagatcatct
    35221 cactttagca gagaaggaag gcaaaaacct atttttgaat ctgtttatgg attacgaaac
    35281 ttttggagaa caccaatggg catccactgg tatttttgat tttatgcgtc attttccaga
    35341 tgcagtgtta tccaagtctc atcttggttt tgccaatcct aaagatgttt ctcgtttggc
    35401 aaattatcaa caagaatctc tatctttccc agaacctgta tcttgggcag atgaacaaag
    35461 agatctttct gcttggttgg gtaatgaaat gcaacaaaat gcaagtgaaa cactttatga
    35521 actgatcaat cgcattaaag aaaaaggtga taccgactta ttaagaatgg ctcgtttact
    35581 tagcacttcc gaccattttt actatatgtg tgaaaaatat tttcaggatg gcgatgtgca
    35641 taagtatttt tctccttacg attcacctga tcaagcatat atatattaca taaacgcact
    35701 tacagaattg gaagaacgac ttttgaggta acagaatgaa aggtaaaata ctaatattag
    35761 aggatgatgt agttctttct gaacaaatag ctaacataat gagacattat gaatacgaag
    35821 ttctactaac tgttaatagc gatcagttct ttgaaatttt aaggaatttt cagccagatg
    35881 ttattctttt ggatgtatat cttgttggct ctcgtttaaa tggtatccaa gttttaaaac
    35941 atctacgaaa taatatggat ttaaactata aggtaatagt gatttctggg gaagttactt
    36001 cctcacaaat aaatgaaatt cgagcactgg gtgcttatca ctttattgaa aaggggtcgg
    36061 ctttttctat aaatcaattg ttgctccaca ttgaaaatgc tattactttg aagaaacaag
    36121 aagaagaaca tatcggacta cagattgaat atattaacct caaaaaacag tatacaagaa
    36181 gtttcccttt catcggtgaa agcactgcca taaaaaaagt acgagaacaa attatccgct
    36241 tagccactgt agatgaagat atatttttaa taggtgaaac agggacaggt aaagaaatag
    36301 cagcaaatta ctattacata tattcacctc gttttggtaa accgtttcat acggtgaatt
    36361 gtagtgcact atctgaatcg ctgatagaat cagaactgtt tggtcatatt aagggttctt
    36421 ttaccagtgc agatcgtaat aaaataggtt tttttgaaga atgcacaaac ggtgttctgt
    36481 tcttagatga agtaactaat ctttcattaa tgtcccaatc caaaatccta cgcgctattg
    36541 aaaacaagga aatccaaatt gtgggtggaa acctcaaaaa agttaatacc cgacttatat
    36601 tcgcttctaa tgcaggatta gaaaagctct cggatcagag cgtatttcgg aaagacctgt
    36661 tttatagaat tgaaggtaat attatagaat taccaccttt acgagaaaga gatgatgata
    36721 ttctgctctt aatctgttat ttcttaacca acttttcttc tcaatacagt gttatggatc
    36781 aattagacct gcctgcgtta aaaaatgact tgctgagtta ttcttggcct ggaaatatta
    36841 gagaactccg aaatttctgt aaattcatta tgataaacga aaataggatt aataatagta
    36901 taattaaaaa acacttaagc cagaagataa gccatgctca ctattcgggt gatagtaata
    36961 tggaacgata tatgcgcatt gataatctga aagatagcgt ggcttcattc gaaagagatt
    37021 atttattgca ccatttacaa ttaaacgaat ggaatgtttc tcaaactgca caatcaatcg
    37081 gtatagaaag aactaccttg tataagaaaa taaaaacttt ggggattaat cagtcggata
    37141 tggagagtta aaatgctaca ggaaaatata gggctgaaag tactttcttt gattattgct
    37201 atctttgtgt ggattcagtc tttattaatt tctgaacagc gtagtgttgt aaatttgcca
    37261 ttagctttac tctctattcc ccaaaatatt actcttgaag atttcccaaa aactattcct
    37321 tttcaagtga gaggaagagg attggagatc ataaaattga aactctctcg cactaaggtc
    37381 tttattgatg caagtaaaat caaacctgga acagatatta tatctttgag cgattatact
    37441 ataaatctac ctgataatat tcaattggaa ataataggac cggtgaatac acaagaaatt
    37501 gcagtgcact cagatttatt ttatcagaaa aaagttccag ttgagcttac tttctctgat
    37561 aaccaaactc gagaacgatt ttactctatg aattattcaa taatacctga aagagtaact
    37621 gttttcggac ccaaaagtaa agttcattta attgataggg tatcaactga atcgattaca
    37681 gcttctatgc tggatcaaag tgaattaact cttttgctta atcccttagg aaatgatgtt
    37741 tcctcatctg taaaacagat taaagttata atttcgtcct cctctaatac aaccagagtt
    37801 atcgataatc tacccataac tgtgacccaa gagaaaaaat gtataccttc tactgtaact
    37861 gtgaagatat ctggcgattc taaagaaatg attaatatca atccttcaga tatcaacata
    37921 ggaatcgcta atgaacctga tgaatatggt ttttattctg tgactgtaaa aattccagag
    37981 ggattacaat taattgaagt tacacctcaa aaagtacgac taaaataaat gatacctcag
    38041 tatattcttg cctttgaatc ttcctgtgat gatacttctg tagctatttt ggatacagag
    38101 tataatgtaa ttgtaaactt aatctccagt cagccagaac atatagaatt tgggggcata
    38161 ttgcctgaaa tggcttcgcg cttacatcta aggcatatac taacattaac aaagaaagca
    38221 cttgatatat ctgaacttaa cttatctgat atcagtgcga tagccgtttc tattaatcca
    38281 ggtttaattg gttcgttaat tgtaggtctt gcttttgcta aaagtttagc ttggagttta
    38341 tctttacctc tggtaaccgt aaatcatatg cttgctcata tatttgccaa ttgcatcgag
    38401 cataaagaaa ttaagccgcc ttttttggct atggttgttt caggaggcca tactgaattg
    38461 gttcaatttg ataccttagt ttctttccaa atagttggaa aaacattgga cgatgcagct
    38521 ggagaaagtt tcgataaaac agcaaagtta ttgggcttag gttttccagg aggacctata
    38581 attgatgacc ttgctaaaaa tggtaatcct gattttatta ggtttccaag ggctctaccc
    38641 caaaaagata atttcaattt cagttacagt ggattgaaaa ctgccattcg tacctggctt
    38701 gtaaatcaaa aaccagaaat tctaaacgat aaacttcctg atattgctgc ctcagtacag
    38761 caggcaatta tagaaccctt agtcaataaa agtgtgctct gggcaagaca acataaaata
    38821 ccctatatac ttttagcagg tggtgtagca gctaattctg cattgcgtaa acaggtaacc
    38881 caatccgctg caaaatatgg aatcaaagtt ttttatccat ctacagcttt ttgtatggat
    38941 aacgcagcta tggttggtgc cgctgcaatt cctaaatttt tagtaggaga ctatgcaccc
    39001 cttactgtaa atgtttcttc caaaaaaggt acgcgcacag tttagaatta tcttttaaga
    39061 ggtatcgtgg gtaactgtac cctttttaat atcttatatt aagatgttat atttaagcta
    39121 atacaatgga gctaaatggg ggacttgaac ccccgaccta ctgattacga atcagttgct
    39181 ctaccagctg agctaattta gcttttaata atcttaaata tagtctgttg cccaggggca
    39241 tctataaaat atccttcagt tgttcgcaaa ccatcttgtt cttttatagt acattgagtt
    39301 ccaagttcca ttgtatagtt ttttgagctt agcaagcgag cataaatttt acttggagat
    39361 accaaattac ggggaaacca gttctgactc gaagagaaag taacataacc gcgaatatca
    39421 gattctttta tccattcggg gatagggaga gaataacgca atttgaaatt gatcttacca
    39481 ctgaaatttg gtagataaaa gctaagacaa ttcacattgt ttttttcttt cagcatttcc
    39541 ggaaaaggaa atataggatt aaagtgttcc gggacaagct cgcttgcata tatgcaagga
    39601 gcgaactgat cattaaccat aagttgctct attatagcat ctttcgttaa aaaaatgcta
    39661 taataggttg cttccggaag agcatcttta tccactgaaa aatcagctgt tatataaatt
    39721 ctttgcatag gagcaaaaat gcgcaattcg agatttatta tctcaggatt taaaagggca
    39781 aagtttatat ttaaagtatc gacttcttgt gccataaggc atagagagat cactagcaga
    39841 ataatagtta taaatatctt acgcatttca tactcctgaa ttaatttact tttgtttcat
    39901 aacatttata atagactatt tcgtcaagat aattcctgct tattttagga ggacagcttt
    39961 acgaataact tgattgtatc cgcaatctag tttgataaaa tagattccag aggaaactga
    40021 acggttggaa aaatcacaag aattccagac aaagctatat tttccagctt ccagattgcc
    40081 actataaaga tctttcacca attggccttt aatattatat atttttatag tagttttacc
    40141 agcagcaggt aaagtaaaat taattgaagt attaggatta aatggattag gactgatact
    40201 aaaaattagt ttaggtttct cataatcaac tattcgacta gtatctgaac ttccgtaact
    40261 tctttccaaa agggaaaagc tcatagattt aggagtatct gaggttctac cttgtatcca
    40321 atagtaatta ttaggccaag gaatattaac atatgtatct acaatcgacc attcagtcca
    40381 ttcaattagt ccgacattat caaataaggc agttccatta ataccagtgc ttcctgtcat
    40441 ttgtaaacgg atatcataat aatagacatt tgatggaagg tttagttctt taaaataata
    40501 agtccattca gttgttccag atagattatc tataacataa tcagtataag atgatgaacc
    40561 agaagttctg gaagtataaa atcttatcat tatattagcg ctataaacat tttcggtctt
    40621 tatccatcca tgcagagtgt attttttgct gttatcataa atcttacaac gattagaaat
    40681 tgtagaagtt acagtagaag aactggttgt gctaagtttt gcgctacggg ttccatcaat
    40741 aaaatctgat gtggaataat cgggtggaac ccaaagagta gaaccctcat cttcaaaatt
    40801 accaaaccag atattttcct gtcctagacg aaattgatac tcagctggag gctcaacatt
    40861 tgttatctcg catatagatc ctcttcgagg aagttttata ggtggagtac agctcatatt
    40921 ttcattaagg ttttgcagct gtttatgaca actaaaagcg ttggcaacta caggaggata
    40981 atcatcatcc attaaaactt gggcttccaa tgctatttta tctactgaaa cacgggtatc
    41041 aagatcacga gacctgctgg ctatataatc caaaatataa atagcaagct gtccagttgc
    41101 tggtttagga atgaattgat caatataaaa aggtttgatt cggaaatttc taaagccatc
    41161 taaataagca tctgcataga gtatcatagt aggcattgtt tctggataat ccaaatcaaa
    41221 tacaaagttt cccaaagaat gagcaatgag cttattttga tatagttcca gaccttgaat
    41281 aatatgtgga tgatgaacta ttactaaatc ggcaccactg tcaatggcac tatgacgaat
    41341 ttctctatcc cattgatgag gaacatcatt ccgataacaa taatcctcat cttccgtgtc
    41401 ttctaaaaaa ggattaagct ctttgtcata tccagcgcca ggagctaaag aatattcact
    41461 acctccatgc atttcaacaa ttttgaggtc ggcaatacct tcaactgctt gaatttgttg
    41521 ctgtatataa taaggagtca tataagcaaa gccgggttta cagtaacctg cctgtaaaaa
    41581 aggttgagca ttattatatt ggcctgttct atctgaactt cttaagaagg caatatttat
    41641 acctctacag ttataaaatg caggaagata tgcctcatac gcatttgctc cacacccact
    41701 atatataata cctgcactat caagaaaacc ttgcatttgt tgatatgctt caattccata
    41761 atccatagta tgattattgg ctgtacaaac aatatcaatt cctgcataaa ccaaaccact
    41821 tacattagca ggattacctc tataataaac actttttgtt gggtgatgaa ccccaacatt
    41881 agataaaaca acttccaaat ttgctatatt aatatctgct gcatcaccaa aataaggttt
    41941 ggttggatta aaaatagcgt ttactcctaa agtgggtata atgcctcccg ggttttcata
    42001 tcttcttgca agcattatat ctccaacaaa attacaggtt acaggtaaat ttgagttacc
    42061 tgtttccaat ataatatcag ttgaagcaaa accaactttt cctgtatcat cagtcacctt
    42121 tagagttaca gtatagggat aagaagatcc tattgtatat agatgatatg gattgggtaa
    42181 attgctatat gtagaatccc caaaagacca ttcataggta aaaacatcac tatcaggatc
    42241 aattacatcg ttgtaaaaaa atacacctgt atcttttact tgataggatg tagatatgct
    42301 taccacagga gcaaccggaa gatcagaact aatatcaatg agattatcaa accagacact
    42361 acgcgaagaa aggccatcta aatcattgat ataaatgatc ccattgataa ttggatgata
    42421 accccaaaac gcctgccaat cattacttat aggaagctga attgtgttcc aaacattatt
    42481 aataaaagca ccctgatata ctggaatcca aacctcaata tccaatattc tcgttccaga
    42541 aaagctataa aataaactat gcataccatc tgtaaaacct atgccctgaa tacagggatt
    42601 tgatgtagtt ttaacttgta tctgaaaaac attggtgctg tctataggat agggagttat
    42661 ttgctgaagt ttgtaacagt taccggttaa ttgtaaacag taggaactac catctggagt
    42721 tgatgtacta agagtccagg catccggttg aacatcttca tcactccaag aactcaaaat
    42781 gatcgaacca ctttcaaaat tttccaaggt tgtatagcga atagaagcta tagcaatgtt
    42841 tgcgtataaa aagatgaaac cacatataaa ccatcttata ttcattctat cctccccttc
    42901 ccctatttat tgcttattgc tatattcaga atataaaata aagcattttt taagccagac
    42961 aagcggattt gttttctttc atttgcctcc cccgtaagcc aatattaata ttatataagc
    43021 taatattata taatgtctta tctgctgact attgaaaact gggattagtt ctccacaagg
    43081 atcgtagaag ataaaataga caataaaaag gtatcattct gtcttgggca atctatatca
    43141 taaatggttc catatttttt aaagagctta ttatgaagaa gtaaactatg tccttatcca
    43201 atatagagat tatctataac cctaaagaag ttatgattcg tattatgcta atccagttct
    43261 gatatcggct ctgcaatttt cagatggaag agtggctaat accatttcgc tttcacatat
    43321 ccaaattaag ctatctggta taagtaaact ttgagaacat ttccttgttt tcatcattat
    43381 cgtttggaga aacctatacc taatcattca ataataagac actggtatag aatattatcc
    43441 gaatataata tctttaacta atctctaaga tgaaattaat cagccctttt attctgcttt
    43501 cagttcgcga gtcattaatt ctacaattgc ctttctggga tcttcgtctt cataaagaac
    43561 ctgatatact ttctcaacaa taggcatttc cacttgcatt tttttagcaa gcagataaac
    43621 actgcgggta gtagtaactc cttcagccac catttccatt tgtgatataa tatcaaatag
    43681 tttcttccct tttccaatct ccattccaac atacctattt ctactatgag gtgaagtggc
    43741 tgtagttatt aaatctccca aacctgaaag tcctaaaaag gtttccggac gcgctgataa
    43801 agtaattccc aaacgttgaa tttccacaat tcctctggtt agtaatgctc cgattgtgtt
    43861 atctccaaag ttcaaaccgt aaattatacc tgcagctatg gagattatgt tttttacagc
    43921 acctccaatt tccactccga caaggtctga attacgatat actcgaaaat aagaattact
    43981 aaaaacaatt tgtaattggc aaagtaaatc ttcatcgcta cccgcaataa ctaccgttgt
    44041 aggaattttc ctggcaactt cttctgcatg agaaggaccg gataaagcac aaatcttgtt
    44101 ttctggaaaa aaaggcaatt catcttttat tacgatatca agggttttca aactgtcttc
    44161 ttcgatacct tttgctacat tcacaatcgc ttttaaagat gggctatgcc ataaagaatc
    44221 tggggtattc tgtaaggttg ttcttaaaaa ctgtgaagga gtagctaaga taactatttc
    44281 cggagaaaaa atgacaatat ctttaaaatc attagtaaaa tcaacttctc caggaatagt
    44341 gatttctggt aacagatgtg ggttggaatg ggtttctttt aacagtgtta tatatacagg
    44401 attaaattcc caaactaaaa cttgatgtcc attttctgat agcaatgctg ataatgctag
    44461 tccccatccc ccacctccaa ggatagctat acgcattatt tatccttatc atcatgattt
    44521 atatgaaaga aacggtttaa ttcttcaata tcatcttcgt ccagatcaat atcgtccaaa
    44581 tcttcttcgt cgctatcttc tacccagtcc ggacagaaaa gaaggtcttc ttcgaaatcc
    44641 caaccgtcaa cattttccag cccataccgt gataggaggt catcttccca aaatgaataa
    44701 tatacagttc cctcttgaac agcccaaacc agaacgggtc ttttatagcg tcgcaattcc
    44761 actttgggat tttccactaa agttaggaat tcctttattt tcttaggatc gctttccttc
    44821 ttaaatacca ttttaaacct ctatgctcaa aatttgaatc tgagagcagc gcttaaacga
    44881 ttttcattat attctttcaa acgcaacact gattcattat cagtttctac atttctgaat
    44941 aagtgagagt agtcaagaga taaattccac tttggtgaaa ataatatatt cattccagct
    45001 gataatgtat aagtataatc ggctctacca ccataccaag aacctcctcc ctggtaatat
    45061 ttctgttcat aattgagtgt tagtgaaggt tgccaaagag tttcactttt tgattccagc
    45121 ggcattttcg gtaaaacaat ctttcctttg taggtattac tttgatagga gcaatcttta
    45181 ttttcaattc gtttatcgca gctgaaatct cgataggcat aagaaccttc tatagtaaaa
    45241 tagggaaatt tataacttac gcccaattct tcagcaaaag ccttaccatc agcttcggtg
    45301 aaatattcat tataacgata tatatcatag cgcagatctg ctttcagtgt cgttttagga
    45361 aatggtttta cagctaggtc tccccgaaat gtatcccggc tataattgta tttttcaagc
    45421 tctttagttc cgtccgtatc aataaaatga cgaaagtaga tataaggact gcttgcatac
    45481 tgaatgctgc aattccaata atatctatcc accctgaatt tagctgccca atcgtagcga
    45541 tatttatctt cattactaat attttgagat agagtacttg ttatagaagg ggtaaatttc
    45601 caccaacgat aagacatagg ataacccagt tcaatgcgag tgctaagagt taaatcatcc
    45661 attgtatcca caaaatccag attcgggtga tggtcgtcaa aacgattaat atcatattca
    45721 gatagatgaa atacattatc tgaataagta gctccaatct ttacagaagc tgtaatttgt
    45781 gcatcaatat ctatgaagcc taagaaaagg cataaagaaa gcagcataat aataggtcgg
    45841 atactggttt tccttcttat tttatgcttt tttggtatat ttcttttctc ttgcatcgtt
    45901 gatgaagagg attcatttga atctactatt tcttttagtt ctttaatcat atcaggtttt
    45961 gtatcaacgg aaggaatttt agagtttgaa ttcgacataa tttatctcta cttcttcaat
    46021 agaacaggtt ttgcaaggaa taaatgatca cttagagcac gcaactcata attggtactg
    46081 ttagtatttt ccggtaaaac gatttttttg gcaatcccca agttgttgat accctggcta
    46141 tgatattttg tagtgcgctt ataggtaaaa tctatcactt caaccaattc gccatttaca
    46201 taaactccca gttgaggtaa agacctatcc aataaacgag gacgaatata aacataggca
    46261 tttcgaccat tattaatagt gaacttaaaa ggttgatcag gcataaagct ataataatct
    46321 gtgcttgagc tattatgctg cacactaacc actccactat gtgcttttat aacaaggttc
    46381 ggttgttttt taggtttggc aggtttaggt ggtaaaacac taaaagccct catatatata
    46441 gagcggtcat agcaaattac ttttatctct ttagtgcctt caggaatatc taatgtgaag
    46501 ggattataga tataataatc gcccttcttt tctttcaacg atagttgata aactttccgt
    46561 tctttaccaa taacagaaac aatttcaggt ttacgcactt cgccgtaact aaaactgcgg
    46621 atttcaattt tatcaactcc ggtagtattt agtattaatg ctctttcagg taaactgcgg
    46681 taataataat agtttcctgt ttctgtttta agaaggcgtt tagtgcctgg attatcgaaa
    46741 ggaagaacct tatatcgatt cgctgtctgc gcaaagagta tattggcaaa aaggatgatt
    46801 agtatgatag aaaaatgatg tttatgcatt tttattatcc cttacagttc ttcgtccgga
    46861 tctactatgt tagccataaa cctgcgatcg tgtctatcaa atatgattac catcgcaata
    46921 gcaataacta agatagccag acaaataata ttgacggttt gattatgaac aagtgaacta
    46981 aacattttcc cctgtcccac ttttggtata gtatcaaaag aagtaggaag accatatctt
    47041 ttagcccaac gtaaaatacg gcgaaaatgt aataccggta tattgttttt cgccatttta
    47101 atcatcaccc cttctttttc ataactatgt tcagctaaat ttcggttgat accttccgga
    47161 atgagattgg cattgggttc gctacccaca ttggcgatac cggttccaat atttataaat
    47221 gctttgtagc gttgaccttc tggtaacatt tcatcataag cggccattct aagagaaatg
    47281 ttatcatcca gattattacc tattaataga ggaacattat ttctttgcat tgcatctcgt
    47341 agtgctttaa tcccgttatc ggataaacca attcccaaat cttcttttcc cccaaaggag
    47401 gcgtaggcag atttaaaatt aattaatcct ttttccttta atatgttttc catatctaac
    47461 catgtaagtt ccggacggtt agctccataa gaagctgaag ataaagaaac aattattttg
    47521 ggattaagtt ccattacttg tatcgcagaa tacagagcaa tatttacagc aggattgctt
    47581 cccgttattc ctacagctat acaatctcca ggcataagtt tcagtttgga tagttcttca
    47641 acaaagatag cagatatatt aggatttata gcagcttgtt tttcagacaa tagacctcta
    47701 tctgttgtaa tagaactaag acggtttcct attaaaccgg tttttaacgg atcatctatc
    47761 gggtcaattt ctatccctcg tcgatttatt tctgcagtta acgaatcaat tgatgctttc
    47821 atcaaattgg cagcagctat ctttaattca taattatctg atttgatgtg tagataactg
    47881 ctttgtgcaa tataaaataa cacaatggct aatatcaata gaattatcag ggaacgatca
    47941 gatttcagtg aagggcgata cataattata tctctccatt aaatattacc atcagggcaa
    48001 gtcgaacaag tattgctgct atacccattg aagatacggt tttccaaaaa ccttgacgct
    48061 caaaccaatt ggcaataagt cctggtatga tataacctat agattgcatc tgcaactggt
    48121 gaacggtaat atcttgaaat acaaaaatgc gtgaggccca tcctaggata aaacctatta
    48181 atatacttag aaccatacgc cttttaccaa acataagagt aaaactacta attatacgga
    48241 taattcccca agtgattaaa ctaataatta gggtgcctat tatctgcaaa ggcttatcca
    48301 gatacaaggc aatataccct ggtactacta taccccctgc agaagcacca agtaattcac
    48361 tgaacattag tgagacaatt acccccattc caaccgcggc ttgaactatt gtatcaatca
    48421 catccggctc ctttctatat tctctttcat tttatgcttg aaatgagcaa ctatctgtgc
    48481 cccatattta atatcgcctg ctatgtttcc tattcccaaa atgtgggatt cgctgttagt
    48541 aagctctata accttcttat aaataacttc tggtaaaggt tcacctaaag ggatgatttt
    48601 ttctttagga ataccggctt gcaaacaata agattcaact ttttcaggaa tttctccagt
    48661 tagtaaaaga taagagaaat cgagatcttt cacagcatcc actaattgat ggctacgaaa
    48721 gagacgatct gctctactgt tcaaaattat aattttttcc acattctgca aatgaccagt
    48781 aaccatctta attattccta ctgtggattg gggatcatta gcggcaaaga cattataaaa
    48841 aaatagagtt ttgtttccat cctgtatttt gtatttcgtt aaagccccag gatcaggagt
    48901 tgccttctgc ataccatgta aagctatttc tctctcaatt cctgcttctg cacaaactgc
    48961 aagcgcaagt tgaacatttt ctgcgtgctc aatataacga aatttatcta attcctcttg
    49021 agtcacatcc ataggacgaa ttttatgaat acggcatcgt ctttgctcgg ctactttttt
    49081 caatagtgaa aactgttcat cttctgctgt atagcaaact ccatgtttgg gaattgtatt
    49141 acttaaacac atagtaacac tttgaacagt agaacccatt aaatcaagat ggtcttcccg
    49201 gcaatttgtt attacactaa ttgtactacg aataaactta cgttctgtta tccattgtaa
    49261 aacggggttt acagccatac actcaattac aatagcatct ggattgtgct ttacagcatg
    49321 acgaaaaatg tatttctgtt caattatgtt tgcacccatt agcctgatta cggcagcttc
    49381 gcgtccatca ggaagaataa ctcgaggtaa agttccagta gtttttgcga ctgtagtttt
    49441 acccccttcc cttaaaccag ctgcaatcaa acgggtaaca ctggatttcc cacgcgtccc
    49501 atttacatgt attcgaatag gtatagctcg aacatttttt aaatgtcttt gatattctat
    49561 aatccaatgg gttatcaata tcagcgtcac tattatcaga acagtcattt actcccctgt
    49621 tattaattat atttatgcac tttaagctgg gtgccattaa ccaccaaact gtgatattat
    49681 ataaatgcaa ttaatattcc atttatctct cttttgtaac ttagctatcc aatttgggct
    49741 attgtattgt aaggatacct attttatgtt tctttttttg aagatgcaga cccataaata
    49801 tataaaatag ttagaaaaca acagtatccc tcttttctaa gaatggttaa aggaaaatgt
    49861 acaacaataa ctaatatgta ttgaaatgct gtttcccttt tcgtactgta caaaaagtca
    49921 caacttgcta taataaaatg ttcttgtagt atttggtact tatatctcag caataattat
    49981 actaatctat aattctcttt tcaaacggaa atgctttcag tgtttttccg tataggcaac
    50041 cgcaaagctt ttaaccaagc tgttttgagt aataagcata acatattcta tgcaaggaaa
    50101 acagatatca ggttgtagta atccctcatt actatggaaa aagctaaata caagatactt
    50161 gacagttagc ttaatctgta taaatggata tgatgacatt aaaggattaa taatatgaag
    50221 atagctactt ccccaaaagt actttattac catatagtcg cggatagttt tccagagatt
    50281 tatccgcagg gtataagtgt atccagtttt tttacacaat tgagagcatt tcagcaaaaa
    50341 ggatataatt tccgtccttt aagtgaagtg ttggcagatc cccaacaatt tgatggcaaa
    50401 gatcttgtta ttagtttcga tgatggtttt gcagtaaatt atccagtatt aatgtatctg
    50461 agcaaagaat ataaaataaa acccacacta tttttaattg gtaaatgcat agataacaga
    50521 gaaatggctt ggaatcataa actcatttta ttaaaacagt attgttcatc agaaaagctt
    50581 gcattaataa ttgacaacta tgttcccgga gcaacgccag catcgttatt ctcttgcgtt
    50641 agcatgtctg ataaggatga gatcgctaat tctctttgga acgagattat gcctttcagt
    50701 gagaaggact atctgaataa tcataaaccc ttcctttcta tttctcagct tgaaagttta
    50761 cttgatgcgg gtgtggaatt gggaattcac agctggtcac atccggattt ttctcgttta
    50821 agttatatag aagcatataa tgaacttgag cagtgttttt ctttgatgga agaggttaat
    50881 ttaccttgcc atagatattt tgcttaccct tatggtcgtc ctgctgcaac agctgtcgaa
    50941 tccaaactta tcgaaaaaat aaagctttct gcaactttcg gaattagcta caaactagga
    51001 gataaccgtt caggagatag tcgctggcaa aggactaaaa tggacggaag caaaattatg
    51061 aattgggaac agttttattt gagtccctac ttgagattat taaagcatcc agttcatgct
    51121 cttaaggatg ttctagaatt tttatgattg acaaatttta tgaatataac aaaatgattt
    51181 tagaataaag aattaatcct tagcttccaa gctaataata agttagaggt tgtttatgac
    51241 atcaatacct gtcttatggt tgatagctcc tttaggtgct gttttagctt tgatattcgc
    51301 agcagtgttt ttttatcagg ttaaaaaaca agaccccggc agtactgaaa tgcaagaaat
    51361 tgcaggttat gttcgggaag gtgcttttgc ttatttgaaa cagcaatata aaggtgtagg
    51421 catttttttt ataattgctt ttatcatttt tcagattatg tcattgggat tgggtgtgtt
    51481 gcattggtta atcccctggg cctttttaac tggaggtttt tttagtggat tggctggcta
    51541 tattgggatg aatatggcta cacttgcctc taatcggaca acacaagctt gcaccgtagg
    51601 tcttaataaa gggttaagaa ttgcttttcg agctgggagc gttatgggat taacggtagt
    51661 aggccttgcc ttaattgata tatcagcttg gtttctcatc ctaaatttgt ttcattttcc
    51721 tctatcacaa attaccgtaa ttatgctttc ttttggcatg ggcgcatcca cgcaagcact
    51781 ttttgctcgt ttaggaggag gcatttttac taaagctgcg gatgtaggtg cggatttagt
    51841 cggtaaagtg gaagccaata ttccagaaga tgatcctcgc aatccagcta ctatcgcaga
    51901 taatgttggt gacaatgttg gtgatgttgc tggtatggga gctgacttat atgagagtta
    51961 tgccggctca attttagcta cagctgcttt aggaatgagt gctgtaaata tgttaggtgc
    52021 caattatgct tcatgggcta ccaattttgt ggtagcacct atgatcctcg ctgggttagg
    52081 agcccttctt tctattttgg gtattttcac tgtaagcacc aaagaaaatg ctggccccaa
    52141 ggaattgatg tttgctttaa acaaaagcgt ctattccagt tctttgttaa ttgcgatatg
    52201 tgcattcttt gtaacaaaat ctctgttacc tcctgattat tattttggag tttttatctc
    52261 tacagtaatt gggcttgtag caggaatatt aattgggctt tttaccgaat tatttacctc
    52321 tcatagctat aaaccaactc gggaaatagc taatcaagca gagtatggtc ctgccacaat
    52381 tattttagaa ggtatatcag ttggaatgcg atcaacagct gctccagtga ttgttattgt
    52441 ggtaggaatt cttgctgctt ttatttgcag ccatggtttt tcttctattg aaatgggttt
    52501 atatggaatt ggttttggag cagttggtat gcttgctact ttaggagtaa cattagctat
    52561 ggatgctttt ggccctattg ctgataatgc cggtggcaat gcacaaatgt gtaatttacc
    52621 tgaaaatgtt agattgatga cagataacct tgattcagtt ggaaatacaa cagcagctac
    52681 tggaaaagga tttgctattg gaagtgctgc tttaacagct atggctttgc tggctgctta
    52741 tttggaagaa gttcgaggag cactaacctt gataggaaac aaagctggaa aagcagtttt
    52801 tttacataca aattataata tgaaattcag tgaaaacatt ccaatcgcag aggcttccat
    52861 ggcggatttt atacattact tccaaatcca tgtgttgaat cctaagtttt tacttggcat
    52921 tttcattgga gcaatggttg cctttttatt ctcctcatta accataaaag ctgtaggacg
    52981 cgctgctggt aaaatggtta aagaagtacg acgccaattt aaagaaatac caggaatctt
    53041 agaaggaact gctaaacctg aatatgctaa atgcgttaca atctccactc aagctgctca
    53101 gaaagaaatg cttttacctg ctttaatagg aattctatct cccattgcca caggactgat
    53161 tttaggaatt cccggagttt taggaatact ggttggtgct ctttcctccg gttttgtttt
    53221 agcagtaatg atgaataacg cgggaggggc atgggataat gcaaaaaaat atattgaaac
    53281 agatctgaaa gctaaaggta cggatattca taaatcaagt atcgttggcg atacagttgg
    53341 agatcctttc aaagatacag caggaccttg cataaatatt ctggtgaaat taatgagtat
    53401 gatttctatt gtcttttcag gattgattgt aagttactca ttgcatgtgg aaaatataaa
    53461 ccctcccttt agtaaggcgc aattaaatca taagattgaa aataaaataa ttatggtaga
    53521 taaggattct ctaaatgtaa ctaatatcta tcctgtttca gccccaaaaa ttcctcagga
    53581 tcagtaaatg atcatataaa agaacataaa ttgaggcatt aaaaccaaac aatgtataaa
    53641 tgtgatgaat atcatattct tttaacgcgt tgctaactta caattcggca agcactttaa
    53701 acagatgttt cagagtgatt agtattcttt gccaaataat atgctattct ttcttttata
    53761 cggtaaagaa agtgagtaag caggagaaat atttatcgat tgttacttat aaaaccactg
    53821 cattgtataa accttcaatt ttgctataga ccttacctaa tgggtactta tctcttattt
    53881 ataattgtgc aacacatatt aactggtttc ctgattaaaa aaatgagagt tacaacaaaa
    53941 atatcgttca ttaccatccg cacgacaatc tgcaagaatt aataccaagc attaagtaca
    54001 tttaggataa taaagcgaca tataaagcaa acaaggtaaa tgatatgtta attgatgaaa
    54061 gcaaacaagg ttttcaactg aatatttctc atcatttttt agatcaatat gcttggaatg
    54121 tgagttttaa cttgttcata gataacgacc tagaattata gttgttcaat gtactctgtt
    54181 tatagacatt accttaatat accatagcac aactagcaat gtaacaataa acgagaccat
    54241 ctccacacca taaattgcaa acaaaaagat acaattagtt gtatagttat aattgagttg
    54301 aaaaataaaa gaggcgggaa aactatagtc ctcgcctctc tattaggatg tgtatagtgt
    54361 taatttattg gtgcttatga gttatttctt cggttatttg tccattaaat atagaccgtt
    54421 tgaaataatg agtatgcaac aatttgaggg caagctttga atttggttca cccaagaatt
    54481 tttcgtatat tttcaacact tcaggattgt gatgagacat tctcaccggt aaattgcggt
    54541 cttcttcata taatgctaat ccgcgacgtg ctcttgaagc aatatcgtta ccataaggct
    54601 gtccaccacc acctacacaa ccacctgggc aagccattat ttcaataaaa tgccaagggg
    54661 attcaccttt tgtggcaaga ccttcacgga cttgatcaag aactttgcgt gcgttggaaa
    54721 gcccatgagc aacagctaca ttcacttttc caaagccggg gatatcgaca gatgcttctt
    54781 taaccccttc caatccgcgt actgctgtta tatcaacatt ctcaagatcg gtattggtta
    54841 ccaggtaata agcactacga attgcagctt ccataacacc accggtagct ccaaaaatag
    54901 ttcctgcacc ggtatagaag ctcattcctg catcaggttc ttcatcttga agattagcaa
    54961 aatcaattcc tgtttctttg atcatccgga taaattcgcg agtagttaaa acataatcag
    55021 tatcctgata accgctatca cttagttcgg gacgtcgtgc ttcgaatttt ttagctgtac
    55081 aaggcataat agcaaccgac actatttttg ctggatcgat acctttttcc tgagcatagt
    55141 atgtcttgga aagaactcca aacatactca ttggggattt agctgtggaa acgcaatctg
    55201 ctaaatcagg ataataggtc tccataaatt tgatccaacc gggagaacag gaagtaataa
    55261 gcggacgctt ttctcctgct ttcagtttag taacacattc tgtaccttct tccaagatag
    55321 ttaaatcagc tgtgaaattg gtatccataa cagcatcaaa accgatttta cgaagagctg
    55381 catacatttt tttaggagtt acactgccaa gtggcatacc aaattcttca cctaaagaaa
    55441 cacgaatggc aggtgcttct tgaactatga catgcttatc gggatcgaaa attgcttccc
    55501 aaacagcatc tagctcactt ctttcatgca aagcaccggt ggggcaatat actgtacact
    55561 gcccacaatt aacacagggg ctattagcca ttccgttact gaaagcagta tctacatgtg
    55621 tatcaatacc gcgatctgca aaagttaaag cacaaacggt ttgaacttca ttacacacgg
    55681 ttatacaacg cccacaagaa atacatttat tggtgtctct gacaatggat aaactggaat
    55741 catctgcagg tttttttacc agaatactgg gtaaagcatc aggatccaca cccagattat
    55801 ttgctagtga ttgtaactca catttgttat ttgcagaaca agtaggacaa gttacatcat
    55861 gattggatag aatcatttca actacggttt tacgataggt acgaagtttt ttggaattag
    55921 taacaatttt cataccttca gccacaggtg tacaacacgc acgctttgga gttggatttc
    55981 cactaatttc tactacacaa actccacaat ttccggttgg aggtaaatcg gggtgatagc
    56041 acaaacgtgg aatataaatt ccaattttat ctgcggcttc gataatcgtc attgtatctg
    56101 gaacttccag atggtgaccg tcgataaaga cgtttactgt ttttgccata atatcaatcc
    56161 tcttatctta ttacttttta gttagttcaa tgaattcatc ggggaaattt tcaattgcgc
    56221 tacggacagc cataataggc gattgaccta atgcacagaa agatgtttgc tgcatagttt
    56281 ctgcaatgaa cagcattaaa tccacatctt gcattgttcc atttccctta cggattttag
    56341 aaatcagctt atacagctgc tctgttccat ttctacaagg cgaacatttt ccgcaggatt
    56401 catgacggaa gaaacgcaga atactccaaa gcaaatcgat aatactctga tgttcattca
    56461 gaactaaaat ggctccgctc cctaaaacag cagcatattg atttaagcta gaaaaatcca
    56521 tttttacatc taaaaggcgg tcgggaagaa taactcccgc tgctccacct actaatgctg
    56581 ctttaaaacg aaaacctttt ttcattccac ctgcgtaatc gttgataatt gttcttaggg
    56641 ttgttcccat atctgcttca caaagtcccg gaaaggcaac atcgccaaga attgtataaa
    56701 ctttggttcc cggacattca ggagttccat atttaacaaa ttcttcggca cctttggcaa
    56761 taatgaaagg cacattagcc agagtttcta cattgtttac gatagtggga gctttccaca
    56821 aaccttcaac tcctggaaaa ggtggtttcc aacgcgggtt tccgcgattt ccttccaagg
    56881 attcaatcaa agctgtttct tctccgcaaa cataagcacc tgcaccaatc ttgatttcga
    56941 tatctatatc gaaaccacta tcaaagatat ttttgcctaa aatgccatat tgatatgcct
    57001 gcttaatagc gttttctaaa cgagctatgc agagtttgta ttctcctcgg atatagatat
    57061 atgccttgtt tgcaccaacg gcataagcac aaagcattac accttccagt aattgatgtg
    57121 gatctccttc cattattaac cgatctttaa aagttcctgg ttcgccttca tcagcattaa
    57181 ctactacata tttttgagga acattaagag cagcggtaaa gctccacttt aatcccactg
    57241 gaaatgctgc tccccctcta cctttaagac cagatttctt tacttctgat ataacatctt
    57301 caggtctaag ttgtgttagt gctttttcta aagcttcata accacctgtt ccaatatagt
    57361 catctatatt ttcagggtct attatacctg aattacgtaa aacaatccgg ttctcgtagt
    57421 tgaattttgg gctaaatttc tctgctgcac ctagcattaa acgttttagt ggacgtcctt
    57481 tcaggaaatg ttcctgaaca agttctggaa tatcttcgat cttaacatct tcgtaattaa
    57541 tgttttctgg atatacagtt aaacaaattc ctcttccaaa gaaacctaaa ggtccggttt
    57601 ccaatatatt aatttcctct gccaaatcat ttttggcaag ttcattgcgg agagaagaaa
    57661 taaattcctg aactccagct gcaagagaag tctcattaat agagacaagg atatgattgc
    57721 gatagtattt cattatctca tctccctgat cttatctatg atttcttcaa ctttctcttc
    57781 ggtaagatcg ccataaaaat catcattgat catcatcaca ggtgcatttc cacatacacc
    57841 taagcaggcg cataattcaa gagtgaataa accatctttt gttgtttcgc caacattaac
    57901 accaagcaga actttcagtt tgcgtaatat gttttccgaa cccttaatat aacaaggtgg
    57961 tgattcacac aggcgaataa tgttcttgcc tcttggttta gtactataca tcgtataaaa
    58021 tgtcagcaca ccataaatat gattggcagg tatattaaga tattcagcca cagtatccac
    58081 tgcttcatta gaaatataat gctgaggatc cgcattctga atttcatgga gaatctgaat
    58141 cagattatcc ttatgaggag catatttttt acaaatctct ttgatcatag ctcttatcct
    58201 tttattcgtc ttgattttgt tgttttattg cctccgcttc acgtttagct ttaacatcag
    58261 gatgcattcc cggaacttta gaaattgctt ttacggggca tacatccata caagttccac
    58321 attttatgca ggacagttga ttaattgtat atttatcttc tctgctgccc gaaatacatt
    58381 tagtaggaca ttttcgagag cataaagagc atccgataca acgactttta tctatttcaa
    58441 aatgcatcaa atcgagacaa actttattgg cacagctttt atccctgata tgagcctcat
    58501 attcatctcg gaaatatcga attgtagaaa gaacaggatt gggagctgtt tgacctaatc
    58561 cgcaaagggc aagtttggat atggtttcac ctaagcgttg taattcttca atatcgcctt
    58621 ctttaccatt accactatca atgcgtttta atatctccag catttgtttc aagccaatac
    58681 gacaaggagc acattttccg caggattcat caacgcagaa atccataaag aacttggcaa
    58741 cattgaccat acacttttct tcgttcatta taattactcc gccggaaccc atcatcgcac
    58801 cgcgctcttt taagctctca taatcaattg ggacatcaag atgctgagta gttaaacaac
    58861 caccactggg acctcccaat tgaactgctt tgaatttgtg gtcaccaata attccaccac
    58921 ccacatcaaa aatcagttct cttagagtaa ttcccattgg aacttctact aatcctgtat
    58981 ttctaacatc tccagttaag gcaaaaacct tggttccttt gctggtttgg gttcccatcg
    59041 aagaaaacca ttccgcacct ttaagaataa tagtaggaat attacctaaa gtttctacat
    59101 tattcacaat tgtaggcttt ttcaaaagtc cctgaactgc aggataaggt ggtttaggag
    59161 taggatttcc acgttgacct tcaatagagt gaattagagc tgtttcttct ccacaaacaa
    59221 aggcacctgc gccagttcta acttctgctt caaaactaaa attactgcca aatatatttt
    59281 ctcccattaa acctgcttct ttggcttttt gaatagcaag cttaattcgt ttaatagcaa
    59341 gaggatattc agcgcggata tagaaaaatc cgtttttggc acctattgca taagcagcaa
    59401 tcatcatccc ttctaaaaca cgatgcggat caccttccag taaacttcta tccataaatg
    59461 caccgggatc accttcatct ccgttgcaaa taaaatattt ctcttcagaa ggattatcgt
    59521 gaaccatctg ccactttaaa tgagtgggaa aaccgccacc acctcttcct cgtaaaccag
    59581 atttttttat tacatcaatg gtttgttgag aggtcatagt agtcagcaca gttcccaatg
    59641 cttcataacc acctgtggca atgtattctt caatactctc aggatcaata tatccacaat
    59701 tggccagcgc tactttcacc tgtttctgat agaaagggat ttctttatta gtgagatagt
    59761 ttttttcttc ttcgtcttga agcattaaac gcttcactgg gcgtccctta aggaaatgtt
    59821 cttcaacaat ttcttccaca tcttcaggtg taactttttt atagaaaact ccttccggat
    59881 aaatgaccat tactggtcca tagtcacaag gtcccataca tccagtttca atgatgttta
    59941 tttcgtttgt taaaccttta tctttcagaa cactatgaaa ctgatctttt atctttattg
    60001 ctccagcact tacacatcct gatccacagc agatcaagag gtcaattctt tttaatgaca
    60061 ttttctaata cccccagaac tagttgcccg tagcgacaac ataatcggag acgattctac
    60121 cattaactat gtgttctact attacttttt tcaccttttc aggagtcata ttaccatagg
    60181 ttatttttgg tttaccttct tcgattatat ccactaccgg ttccagggaa gaaagtcctt
    60241 tctctcctgt ttgagtaaca aggacatcgg ttaaatttcg attagcaatc tcttccagga
    60301 aagttttcat aacttcacgg gcacccatag ctattccaga ggttcccatt cctacgacta
    60361 ctttgattct tgtagtccca ccgcgaagtt tcatttcttc ctgagctttt tcacggattt
    60421 tcttaaggtc tgcgagtgtt ttcatcagac atcctccata tttgtatttt gtattccttc
    60481 taataatgat tgatcgatgt attcaataac atcggggtaa gttaaaggta tgttacctaa
    60541 ttcttctttg atagctgcgg tctcgaaata aaagcttttt tctacaccct gttcatggct
    60601 gataagttta atactaaaat caacttcggg gtggccaata ataactccca agagagtatc
    60661 tttcaagtta ccaaggggca tgcgatcgat atggtccaat tgaaaatcca ctgttaaaac
    60721 agtcccaaat ccaggtgcac ttgtcatttg aaagctaccg ttagttgctt ctgcattttg
    60781 ttttaagagc ggtattccca gtcctacttt tttctctcta tcttttttgg aagtatagaa
    60841 gggactttga gctttttcca gagtttgtga atccatacct atcccattat cttccacaat
    60901 taaacgtaat agattatctt ttacatcgcg aataatacgt attttgatga gtttggcttc
    60961 tgctcgaacc gaattttcaa tgatatctaa caggtgtaat gatatatctt gcataccaga
    61021 acccggatta ttgatagcgt tttatgattt cagccacttt gtccttgcta tcaattctac
    61081 cgaatgtgtc ttcaccgatt acaaatacag gcgctaaaga acaagcaccc acacatctaa
    61141 ccgcagtcat agtaaacagg ccatctttag tggtttcccc catttgaatt ccaagctcat
    61201 cagagagcat ttgaattaag cgaggagcac ctttaacaaa gcaggcagtt ccaatacata
    61261 cattaaggtt atatttacca cgtggggtca tcgaaaaaaa gttgtaaaaa gtaacaacac
    61321 cataaatatg acttgcgggg atattcattt cattagcaat gaattcttga acttcgatcg
    61381 gaaggtagcc aaaaatatct tgggctacac gtaagatttc gatcaaggga ttacgaacat
    61441 ttttcttttc tgcaataact tccttcaatt gattgtatag gtcattatgc atctgaggtg
    61501 cggaagtcat aacttctcct tttttttcta tattttttta tctaaacttc tattttccgt
    61561 cctgaaacac cctttgcagc caagatcaat tcttgcactg ttgcttctgc taccgtaata
    61621 atgctataac cttttcctat gtcatttaaa taatgagcat cagaagcacg cagaaagacc
    61681 ttatcctcaa aatcaggatg ttcagtaagc cagcttttta tatcaagcat tgcagttatt
    61741 ccaaaaagtt tcaattccgg ttcacttggc ataaaaccaa gctgactaat aatactattt
    61801 acccctgcat ctatatgagc gggaacaact aatccattat aggcctgaac cttttctgct
    61861 actgtattta agtcccactg tgaggaattt atcagggcac gttgttccac tcttaaaatg
    61921 ttctcgtttt cgtcaatgat aacttgatca ccccaaaaat caacatcatt atttatgggt
    61981 aataagctgt tataaagttc ggaatcaaat tctttagctg aagaactatc atcaaaatag
    62041 gcaagcagat gaatttcttc tatactctgt atttctactc cccaagaata atatagacct
    62101 tcattttttg ctaccatttc ataagcaggg caattttgca tactgttatg gtctgttatg
    62161 gccatccatt gtattccaaa atatttcaat cgctggataa tagctttggg agacatttcc
    62221 aatcctccac aaggggataa tacagaatgg atatgtaaat cagcacgaag cgagagcatt
    62281 ctgaataagc ttatggattg agcagtttat aaagtttgcc agcaagttca aaggtcggaa
    62341 gcttactgga aataagtata atcccttcct caattgcttt ttcaattaca gcagtatccg
    62401 gttcatttcc tttggcaaaa ataatagccg gtatacctgt cattgaagca acagctataa
    62461 tatttagatg tttcataatt gttatccaaa cctgtcctgg tttggcagag cccataacat
    62521 cgcttaacat atcacaaaca taagcaccag ttatttttac tccttccaga gatagcgctg
    62581 aagtacagtt aattccttta atggcagtaa cataatcgct aagattgatc atttatgtat
    62641 ccttattctt tttatcttca tcttcttcag tatctttgct gtgtcttttt aaaagtacga
    62701 cacaatcatc aatagaggct ttacctaaaa caatatcctc ggccagagca taacaggttg
    62761 ggctcccaca agcactacaa tcgagacctg gtagcattgt aagaaattca ttgatctgtt
    62821 tcatcttttg gattgctttt ttgatatctt tgtccagctc cataataggt ctgggagcaa
    62881 gaggaacaac agtaaattca cctttttcat agagttcgta taactcactg gtatcataat
    62941 cctcttcttt gccttctttg atcattttct ttatcctact cattgctaca aaaggatttt
    63001 ccacattcaa acaaccgcct acacaaccat tagtgcaggt tcgtaaaaca atataatcat
    63061 attgatcaag ataatgatct tcaacgcggg agaggatttc catcacattt tcaactccgt
    63121 taacagataa cgcacgaata tcatcacaat caaccaaatc agcttgaact cctgaaagag
    63181 cccaagttaa acctttagga taggtatcga tagatggagg attgttttgc aaatatttta
    63241 acgaatctct aacctttcca tagatcatcc caatggaaaa agctccatct tgaagatgtt
    63301 tataggcacc ttcaggttga tgaacagctg ttacatgagc aatgcaaggc acaataagaa
    63361 aaataccgat ttctttttct tccaaaccat attcttggct tattttgtcc cgtaaatatc
    63421 ttgttaatat actcattgga gcttcctgat gaaataaact gggaagcaaa gatggatatt
    63481 ttaattggat taaacgaaca actgaaggac aattactact taaaataggt cttttttctc
    63541 gattttttct tatataatcc ctaattatat taatcataaa atcagtaaca gatgcttctt
    63601 ctgctacttg atcaaagccg agatccaaaa ctgcctgttt agcaacttca tagctaatat
    63661 cttcggtaaa ttgaccaaaa aaggaagacg aaataatagc tagtttatac ttaaaattat
    63721 gaattatttc cagggaatca caaataggaa ttatagcatg aaagcggcaa atatttatgc
    63781 aattaccaca atcaatacac cgatacggat ctatacttgc tttgtgatca cgaactctga
    63841 ttgcttcagt agggcacact ctaacacagg cagtgcatcc tgtacagtta tcttccaaaa
    63901 tgtggagggc atggaaatat attgattctg tcattttctt attttagaaa aaatattatt
    63961 atttctacca aagtaggtga acccaaagct gattcaatat gcaaggcatc gctgtttttc
    64021 tttatattag gtaaacctaa accagctcca aaaccgaatt cacgaactaa atcatccgct
    64081 gtagaatatc cgggaatcat tgcctgttca atatcgctaa ttccgggacc attatctata
    64141 aattgcatat aaatcaaatc atcatataca ttactaatca ttttaccacc tatagaatga
    64201 gcggcaacat ttatttcagc ttcataacta gcaactgcaa ttcttcgtaa aatatcagaa
    64261 tctactccta agcgttttag taggtttttt agttcagaag aacctcttcc agcaatattg
    64321 aaatcttttt caggtatgtc tatttgcaaa actacatccg gttcgatttt ttttgctaaa
    64381 aattccttca cccgttcttc taaaccggaa ttaatatgcc aatattcagc catctcaaat
    64441 tttgcagcta cgaagtcctg cattatataa aattccactg gatcgatata gagtcatttt
    64501 agtagctatt aatggaagcc cgcgttcctt agccatatca ataacatctt gcattggttt
    64561 tttcccacga acaaaaacaa tacctatcag atcaatcata tcagcaagac gaattacctg
    64621 attatttgtt aatcctgtta aaagtaaagt tggttcttta gtgcacatca ggatgtccga
    64681 aattaaatct gaagcaaaag cacaaggaac tactttattc aaatcagctc cttcatatag
    64741 aacctctgca tccagaagtt cgacaatatc atataaggtc attcattctt tcccttaatc
    64801 aatttataat aaattcagta aatatagata ttgaaattca gtcaagaaaa aaagatgaat
    64861 taaagtatga taaattcata actaaataga caaagatata atattgaact aagaatctat
    64921 tttacagcgc attagcacca agctccctgt accatatatg aatcataatg ttactaagtt
    64981 cttccttagt tgctatcccg gagctgtggt gctgtctctt gggaattttc cacaataata
    65041 ccaccgcaac tctacgataa caagcaagga ataggcaagg aacaagcaat ggttatttat
    65101 tattaaataa taaaaggatg acgatattta cgcaattaca taacgatata atgaagattt
    65161 gcttttggag gtaaatcgat tataatatta ataaaaacag aaggaaggag agtaataaaa
    65221 tggaactgca gtattttgga cattccacct ttcttttcac gaacagccaa aagcaaaata
    65281 ttattattga tccttattta aatgctaatc ccctatctcc ggtaaaatcc aatcaagtaa
    65341 aagcagatta tataatttta actcacgctc attcagatca tatggctgat gccttaacta
    65401 ttgcagatag gcataaaacc actattattt gtgtatctga acttgcttcc atcatttcca
    65461 agagtggatt tcgtacccat cctctgcaaa tagggggtgc ttttcggttt gattttggta
    65521 gagtaaaatt cgttcctgct tttcatggtt cctcgacaat gaacggagtt tatgcaggac
    65581 tagcagctgg agctgtcttt accgttgagg gaaaaactat atatcattgt ggtgatacag
    65641 gcatttttgg agatatgaaa ctgattggcg aaattgataa gatagatata ttactaattc
    65701 ctatcggagg gaattatact atggatattg atgatgccgt acgtgccgta actatgatta
    65761 acccagaact ggccattcct atgcattaca atacttttga tcttattaaa tgtgatcctc
    65821 aggagtttat agatcgaatt gctactaatg gatttcgagg taaagttatg aaacccggtg
    65881 aaaaactgaa tattccataa tctggatatt acctatataa catcataaaa ttcagctaaa
    65941 aattatgtac catttaactt atagtttttt ttaatgtggt ttccccaaac ggattcctac
    66001 tggacgatga tccgaaatag tggaagagta tgtattccag tttagcatcc agttttctgc
    66061 caaaatggtt ttacagtaac tacctccttg ttcgaaatca gcaaaaagtt cattagtaat
    66121 aagaatatga tctatatggc tgaatgaatt gggataggat acattgttat agttgggtga
    66181 gacagcaatt ggcatatcca caaaatagta ttcatccgat ttatttataa agtttaaaaa
    66241 gacattatat tcaggtggtt ctgctatttg atcattcata tctcccaaaa caataacttt
    66301 gctatttgga agattagtgg agatataact atccaatttc atgcaagcaa ggcgacgacg
    66361 cacttcttca tcccaattat tgttttcatc gatataatta tcaccaaaag ctttcaaatg
    66421 attattgatg attagatagt tatcattttc ccaggttata tccagaatat aaggaggacg
    66481 gggaaaggga ttgctgtcat tttcatagat agtatattga ttgtttacgg taactgttcg
    66541 ggtatcatat aaataagcaa gccgataagt atctgtggca gaataaatac aggcgttata
    66601 atttggaata tcagaagcca tatcgataaa tgtagtataa tccataattt cttgaaatgc
    66661 tattacatca acatttaaag ccaatattat ctgttttaaa gcttctaaat ccgttccttg
    66721 aggaaaggtt cgcaaattcc aagttatcac atcaagagtt tgaggagtac caaaattgat
    66781 cgatgaggga tcaatatccg gagtatttag cactgtgttt tttccacagg cagttataat
    66841 cagcaacaca attatcaata tgattattgc ttttctactc atcattagtt agtcctttga
    66901 aatgcttttc catatatact actaatgcaa ttttaatgcc agttgcataa aaatatagag
    66961 aagacaaatt attgtctaac ttggaaggga tattgcatat aatctaataa acactatcag
    67021 gggtgattaa atgaaaaaaa ctatattcat cttaatatta ctagcctgca gtatattagc
    67081 agcaactaca atacaaaatt tcgttacaag tggttcaagt ttatacgctc aatttgaagg
    67141 attgcaaact aatcctattg aatatcaaga tacaaatatg gaagaagatg agccctttgc
    67201 ttccggttca tttattacaa aaacatttgc ctttccatat gctgatgcag aattaattat
    67261 atccagtatg tgttggaatg tttacgattc agaaggcaat tttttatata gcgaacagag
    67321 aaaagagaat agtgctgtga gtattgtgaa cagtttcact tttagagaaa tgcgtggtta
    67381 taccatacgc attgaaactc aaagagaaga agatggatta attcacactc tcaatgaact
    67441 aagtttcgag cttaccggtt caaatccgat tcaaattcct gattctgttt ctccagcttt
    67501 cattgatgct tataaggctt tagcggataa ttttgattat tcgtatttga gagaactgcc
    67561 aattagcaga cctaaaatat tgattatttc tcatagtcaa ctggcaaact atcaaaatgc
    67621 ttatatttcc tggaagaaat ccttgggctt tgatgtgtat actgtaaata aatcagatat
    67681 aggttccagt atccaacaaa ttaaaactta catagcaaat cactatcaga cttataaatg
    67741 cgattatctc attctctggg gtgatgtaac cggaactttt gcaattccta cgaattattt
    67801 tccttcacct gaatatgctg aaaatgatgc agatgataat tattacgcta tgttagaagg
    67861 tgatgactat tttcctgaat tgctagtagg tcgtttttca tttgctgatg cgagtgagtt
    67921 tataacaatg atcaataaaa ccatcgccta cgaaaaagcc ccttatatga cagataccaa
    67981 ttggatgaaa agatctttaa tggttgccgg aaattatgct gaaggcggtt taagaccttc
    68041 cactccaata tctatgtccc gctggttacg aaatagaatg ctttcctatg gttacgcgca
    68101 agtggatact gtatattttc ctcccagttt tcctggaact acagctatcc aacaatctat
    68161 caatcaagga gttcaattca tcagttatcg cggttggggt gatgcaaatg gctggcatta
    68221 tcctcaattt cacatacccg atttgaataa tactttcaac ggttcaaaaa tgcctattgt
    68281 gttttcaatt gtctgtaata caggtgattt tgccaatacg gtaaatccta attttggtga
    68341 gagatggatg cgtatgggga ctatgtccaa tccaggtggt tgtgtagctt ttgttggacc
    68401 gtctgattta cacacaaaaa ctcgtttaaa taattctatt tccagtggtg cttttcgtag
    68461 tatttttgat tatggagtgc gtggatttgg aaccagcgtt atgattggca aaatagaact
    68521 ctacaaaaat tttcctaatg atcttgctcc taatcagtat gtagcattct attaccatgt
    68581 ttataacatc ttaagtgatc ctactttaaa tatgtgggtt ttggttccca atataatttc
    68641 tgaaaatgta attgaaggag gtttaactta taatcaaagt gataaccaca ttagaataaa
    68701 tgctcctcat ttgaatagcg gtataatatc cggaacaaaa gatggaataa attacagtta
    68761 tgctccaata gaaaatggtt atggtatctt acctatcgat cccaatgttg aaggagaact
    68821 aacaattacg attagcaaaa agaactatgt tcctttggta cgcactttaa ctcttcagca
    68881 aaattcaact attggtattg tttctaataa tctgggaaat actattttag caccggatac
    68941 agattattcc ttatctttgc agctaaaaaa tttttccgaa cagatatata ataatgtgaa
    69001 tgctaatcta acaactaatc attctgcaat aattctcagc aatcctactg tttcgattgc
    69061 taccataaat ccagatgcga cagtttctgt ggaatttgaa tttactacca caggtgattt
    69121 tagacccggt gaaataattg attttgtttt atctctggat aatccttcag aaacacatat
    69181 ttttcagcta gtaaccggtg gagcgaaaat acatcttgtt aattatgaag ggattttaga
    69241 aattggagaa gaaaataaca tcattttttc tgtaactaat cttggaaatt atccgatgga
    69301 agacatcaat ctcaacattc aatcattatc tacagctgct gaggcacaaa gtaattctat
    69361 tagtattgga tcacttgctc ccaatgaaac taaacaattt acagctacga taactattat
    69421 ggagggagtt actcctggac ataatattcc attacgcatc gatgcaacca ataatacaga
    69481 ttatagttat tatagttttt atgctgtaac agcaggcaat cctggtgcta ataatcctac
    69541 tggtcctgat gaatatggct attatgctta tgattctaat gatcttggtt atgctcaaac
    69601 tcctgtttat gattggattg aactcgatcc tgaagatggc actcctatag ggaatgtata
    69661 tttaaatcgt gatgacggtg taaaaacgat tacacttcct ttttctttcc gtttttatgg
    69721 tgtagattac aatagtttaa ctatgtctac aaacggatgg gtttctttta taccaacgga
    69781 tgactcaaat ttctataact gttatattcc tgcggcgtta ggaccttatg ctatgatagc
    69841 aggttattgg gatgatctaa aagggatgaa aacaggtgaa gatgaaaatg gagatcctgt
    69901 ttatgattat atgcatatta tttattggta cgattcccaa aataatcgct ttatagtgga
    69961 atggaataaa gcatataatc aatatacaat agatttgggc ccaaatgctt ctttagaaaa
    70021 gttccaaatt attctgtatc caaaacctga tcaggatgga gatattgtaa ttcaatatca
    70081 caccgttgat aaccctggaa ttactacaaa ttattgcact gttggaatag aagatcataa
    70141 tcaattaagc ggtttaactt atactcacgg aaatacttat cctgttactg ctaatatttt
    70201 ggctcctgaa ttagccataa aatttactac caacaaaccg gataattttg tttccaatga
    70261 agataatacc cttccggaac ctattagtaa tctaattaat tatcccaatc cattcaatcc
    70321 aacaactact atcgctttta atgttaaaca accagctaaa gttactatta atatctataa
    70381 tctaaaagga caattgatta aaactctaat aaataatgag ttgctttatg gcgaacataa
    70441 agtagtttgg gatggtactg ataatctggg aaataatatt ggctctggag tatacctgta
    70501 taagatgcaa cttaatggat attctaaaac caaaaaaatg ttgcttttaa aatagatctt
    70561 agcagtcaac agattgagtg aagattatac tgtaaagtag aaatgtgcct gaagctaata
    70621 tgatatccat aagtgtaatt actgattgtg gacagtaaat aatgcatttt aacattatag
    70681 aatggggtgg taaattattt tttgcccccc tttttttaaa caaaatggtt gtgcagcaaa
    70741 atcgtttatt ccatttttct tgacagaact atataaatcc ttttttaggt ataaataaga
    70801 ggttaattat gatagatgta attgtctttt taggaataca gggtagtggg aaagggacac
    70861 aagctgaatt attagcagaa aaaacaggat ttcaacatat aaatataggt gatttattcc
    70921 gtgagcagat tagtaaacaa accgaacttg gcaaaaaagt tcaatccata attgctaagg
    70981 gcgaattagt aaatgatgaa ctagtttttg agcttgttaa ttcatcttta tctacaagct
    71041 gtcctggtgt tatttttgat ggctttccca gaactcaagc acaagcagaa tatttattac
    71101 aacactataa tgttctaaga gtttactatt tggaactaac tgaaacagaa gcaattgaaa
    71161 gaattgaagc acgccggctt tgcagttctt gtggtgcaac ctataatatc ttacataaaa
    71221 aacctacccg agaaggaatt tgcgatgttt gcggctcttc tttaattcaa cggacagatg
    71281 acaatggaga ggcagtccgc aaacgcgtaa acgaatttta caaacaaacc tttgcattaa
    71341 aagaatattt tgaagtcatg gatttgctga aagaaattcc tgctcgcgat tctattgaaa
    71401 ttattcaaca aaaaataatt actgacttac aaactaagct caaaataaac taaaatgcca
    71461 tcagcaaata aaccaggatt ttttaagcga attgaattag ttgcctatct tatggctata
    71521 tggagttgtt tggtcctctt tttagagccc attttcgaat attatgtggg ttcatatcag
    71581 accattgaat tcataactgc tttggccaat attttacttt taatgctaac aattctttct
    71641 cgagttttat ccggtgaagt tcagcaaaat cggacagttg tttattttga tttagtgata
    71701 ttaattttag ggtcattatt gctaatttac caggcaaaat tcgttatctt ctttctgttg
    71761 attcggcaga cctggtttat cgtacaattc ttattattta gagcttttga aggcaaaatt
    71821 tataaattgc taatgcggaa tccgccgatc agtttaatgc ttagttttgc agccgtaatt
    71881 ttaattggga cgatattgtt gatgttacca gcttcatcta cagaaggtta tataacacac
    71941 tttgttgatg ctctgtttac ggctacttct gctacttgtg taacaggact tgtagttatg
    72001 gacacaggaa gttattattc tttatttgga caaatagtta tcttgttgct tatccagatt
    72061 ggtggattgg gtattatgac aatatcaact gcctttgctt tgatgttagg acaaaagatt
    72121 tctttaaatg tccaaacact gatgcataaa gttgtaggtg aaactcccgt aattgatgtt
    72181 ttaaaacttc ttagaagcac agttttagtt accgtaatta ttgaaattat cggagcagtg
    72241 tttttattta ttggatttgc caaagagatg ccaactgctc aagcaatata ttatgctttg
    72301 tttcattcta tatctgcttt ctgtaatgct ggtttttcct tgtggagcaa taatctatgt
    72361 agttatgtgc agtcccctct ggttaactta agtatcactt ttttgattat cttaggtggc
    72421 ttgggttttg cagttttaat tgatatatac cactatttct ttgctaaagg tattattaaa
    72481 aaattgacct tgcattcaaa aatcgtatta tctacaactg ctttattatt aatttccggt
    72541 tttttagggc tttacatttc agaatataac agttctatga aaggttttac tattgcccgc
    72601 agggttctag gttcctggtt tcaatctgta actgctcgta ctgcaggatt taatactata
    72661 gatattagtt tatacagttc tgcttctgta ttaatcactg tgatattaat gtttattgga
    72721 gcttcccccg gttctacagg tggtggaatt aaaacaacaa cttttgcagt tcttatactt
    72781 tctgtggttt ctatgttaac tggaaagcag gatttggtaa ttttcaaacg taaaatctct
    72841 ctttccaatg ctagagaatc aacaacatta gctaccttgg cattgacaat tataatagta
    72901 attttgtttg tgctaatgct cactgaaccg tttcccttcg aacaaatact gtttgaagca
    72961 ttctctgctt ttggaacggt ggggctaagt atggggataa cagctaagtt atcttatata
    73021 ggtaagttgc tgataacttt attaatgtat attgggcgta ttggtccatt aaccttaatt
    73081 tatgcccttt ctattcgtaa tttgcaacct cgtgttactt atgcagaaga aacaattcca
    73141 attggttagg atcaattaac cttccaataa aggagataaa atggcaagat atgctgtaat
    73201 tggattagga cgttttggaa tgacggtagc caagatattg gctgaaaatg gtatggaagt
    73261 tattgccatc gacaaaaata aggatctggt taatgaggtt agcacttttg taacctctgc
    73321 tgtttgtatg gatagcactg atgaagcagc attaagatca caaaatttaa atgaaacgga
    73381 tgctgtaatt atcggtatcg gaagtaatgt tgaagaatgt attcttactg ctgccatcct
    73441 taaaaaaata ggggtaggaa taatatatgc caaagtcgaa tccgaattac atggcagaat
    73501 cctaaaactt attggagtcc aacatacgct tttaccggaa gaaatggtag gtaatcaatt
    73561 agctaaaacc ttaatatcac gtaatgtgtt ggaatatatt aatctctcca gcggacacat
    73621 agttgtggaa atgattgctc ccaaagaatt tgtaggaaag ccacttcagg aacttgccct
    73681 tccttccaag agaggagtaa atgtagtagc aatcaaatat aactatctat cggttactga
    73741 agatggaaga aatgtgatag aaaaacggct aaatgatatg cctggagcaa atgacatcat
    73801 taatgaaggt gatgttctaa ttttacttgg tcctaagggt aatatcgata aattaatcta
    73861 tgaaactgct gttaataagg attgaagata atgaaacgtt ctcttaaagc gaaataccat
    73921 actatgattg ccattctctt tggaatcata gtgttgcaaa cagtaattct gttttttgca
    73981 gtagctcagg caacggattt aacgacccta gcaagtgata ttcattcaat agtgattatc
    74041 tttgtcttca ttatatttgt gtatatagtt gttctctata actatattcc ttatcgttta
    74101 cataaggcaa tacattctgt gcagcaactg gtggaagaaa tatccagtgg caactatcag
    74161 attgatattg acagctcaat ttatgatcag gataaagatt tccaagatct tatctttgct
    74221 ctggaaaaaa tgctgggtat aattacccgt ttcgatcaag ctaaggcaga taaagtattt
    74281 gaacatcatc agcgtattca gtttctaatt aatctgttac ctcagagcat attaattgct
    74341 agttccaatg gagatgtagt ttattgtaat gaacctctca gaaaacgctt tccgatgatt
    74401 tcagaaacag tgaatttgaa tgaattgatt tggaaagatg attatcacgc tcgtgttttt
    74461 tctgttatta tagatgcctt acgttatggt aataatttat acaatgtgat agttgaagat
    74521 gttgtttatt tacacagagc tcgtattaat ggctctataa ttcgcaaccg taaaggtcta
    74581 tctacgggcg ctgtttttgt tttggatttt ttcgataatg aaagataaaa tcagagttgt
    74641 ttatgcagaa ccagaaacac aacgcctgga taaatatctt gtagctttaa aaatacagga
    74701 actctattct cgcactttta ttgaacattt aattgaagaa gattgtatcc tggtgaatct
    74761 tacacctgta aaaaagagct atctgttgag agagggagac gaaatattaa ttcatctgcc
    74821 agaacctgaa ccaatagata tgcaacctca ggatatacct ctagatgtga tttatgaaga
    74881 tgaacacttg gctattataa ataaagaagc tggaatgatt gtacatccgg gttttggaaa
    74941 tccacaagga actttggtga atgctatttt gcatcatttc aaaggaaacc tttccagtgg
    75001 aagagaatgt aatagacctg gtattgtaca tcgtttagat cgtggaacat caggacttat
    75061 tataatagcc aaaaatgacc ccacgcaatc atatcttgct gatatgtttg ccaaacataa
    75121 gattaaaaag acttatttag ctatcacttc aggagttcct gatcccctaa atggtgatat
    75181 agaaacttat ctggcacgca gcaataaaaa tccacgaatg atttgtgtag cgaaccaggg
    75241 gcgctattca tttacccatt acgaaacatt gaaaacatgg cactattttg ctttggtaaa
    75301 agttatacca acaacaggga gaatgcatca aataagagta cattttgccc atatacacta
    75361 tccgctttta ggtgatttac tttataatag tagacgctat gtgcattctt tagtgccaca
    75421 aaatatgaaa cgaaaagtaa cagaattgct tgttaatcat ttacatcatc aagcattaca
    75481 ttcctggaaa ctggaatttg agcatccgat tactaattgt ccaataagcg ttactgctcc
    75541 tatacctgaa gatataaaat acactttaaa ctggttggat gcttattttt gtgttgacac
    75601 agataccaac aatctaaatc tgctcttgga agaggaaaca aaaattggat aaaaaagaac
    75661 aacaaatcga taaaacctta tttgcctcta ttgaagaggc aattgatatt atccgcaatg
    75721 gtggaatgct aatagttgta gatgatgaaa accgggaaaa tgaaggtgat cttttgatgg
    75781 ctgcagataa aattaccccc gaaatggtaa attttatgat cagcgaaggt aaaggtcttc
    75841 tttgtgttcc gttggattat gagatagcaa gtcgcttgga tattcctttg atgaccaata
    75901 aaagtagtga ccgtcaagga actaagtttg ctgtttcagt tgatgttatt gaaggaacca
    75961 caactggaat atctgctacg gaaagagcta aaactattaa agcattggca aatcataacg
    76021 cgattgcctc tatgtttatg cgtccgggtc atatctttcc tttgatagca gaaaggggtg
    76081 gtgttttaag aagagcagga catactgaag cagcagttga tttaacacga ctagcaggct
    76141 taaatcctgt aggagcaatt tgcgaaatta tacgcactga tggagaaatg gctcgtctgg
    76201 atgatcttat tccttttgcc caaaaacata atctgaaaat tatcactatt gaatccctga
    76261 ttcattggcg acgtaaaaaa gaacgcctgg ttcatcctgt ctccagggct caattaccta
    76321 cgaaatttgg ccagtttgaa attataacat atataagcga tatttcggat gaatatcata
    76381 ttgctttagt aatgggtgaa ataaatccct caataccaat tttagtaagg gttcattccg
    76441 aatgtttaac tggagatgct ttatttagta agcgttgtga ttgtggagaa caacttgccc
    76501 atgctttcca aatgatttct gaagaaggaa gtggagtgat tctctatatg cgtcaagaag
    76561 gacgtggtat aggattgcta aataaaattc gggcatacca tcttcaagat gaaggtgctg
    76621 atacagttca agcaaatttg aaacttggtt ttccacccga tttaagggac tatggaatag
    76681 gggcacagat acttaaggat ttggggatta aaaagatgcg gttgatgaca aataatccca
    76741 agaaaattgt tggcctgagt ggttatggat tggaaatcgt agaaagagta cctatagaaa
    76801 taaaacctca agaaaataac ttggaatatt tgaaaacaaa aaagaacaaa atggggcata
    76861 ttctgaatga aatttaaacg cttttgtctc attataggct taattttaca ggttgtctta
    76921 cttttcagta ttgatattcc agaaccagtg ggttttgtta atgactttgc taatctttta
    76981 agtgaagaaa cccgcaataa aattaacgat tgggctattg aactacggga aaagacagat
    77041 gtggattttg taattgtaac tttaccagat atcggagaga tgaatgaagt tgattatggt
    77101 gtaaaacttt atgaaaagtg gaagattggc agcaaacatg atgagggtgt tctagtcctt
    77161 ttagccctaa aagaaagaaa attacggttt gaagttggtt acggttcaga aggatattta
    77221 accgatgctt acatcaacca ggtctataca acgatgaaga gttatttatc caaaggcaat
    77281 gaaaactggg atgaagcatt cattcaaggt tcgttgatgc tgctctctac tattgctaaa
    77341 gaaaaaggag tgactttaac tggcctatct gattatgcta aaaggaaaaa tacaagttct
    77401 tcaggaggat taggcatatt tgctttctta gtaattttcg ttatattaat aatagtaacc
    77461 cgtggcagaa tcttagaatg gcttttgtgg ttttctatct ttggtggtcg cggtggaggt
    77521 tcgtttggag ggaaaggtgg aaattcttgg ggtagtggta atggcgggtt tggtggtttt
    77581 ggtggttttg gtggtttcgg aggtggacgt tctggtggtg gaggaggcgg aggaggattt
    77641 taaatgattt ttttgaagtc acttactttt atcgtgtgga atgttattct tggttgcatc
    77701 ttattgttta tgcttaaatg gcttttgttc aatcgcaaag ccagatacat tttcggtata
    77761 agaattccgc ttactccggg atttttagta gccaaaagag attggctttt cgataaggtg
    77821 cgtgatcttg tgcaggatta tctggatcaa gcagccaatc cttattataa agatggatat
    77881 ttatccaaat ggataaaaca aatcagacat tttatttggg ataagaccga ttttgtagac
    77941 ggttggttct ttatacctgt aaagtttaag ttagtaatca gagataaaat agcagatgca
    78001 ttcacggcag ttgtggaaaa attcctcaga aaaactgtgc ccaggatagt ggaacatcta
    78061 caaattgaac atcagatcga tgaatttgat attcagttga gtgtagattt cttctatagt
    78121 tactttaaaa aatatgttta taaacccctg ttgattggct ttgcagcgct taatttggta
    78181 atcggcataa tgaatatgat ctggttttta attatcgttt agaatatgaa ttttctcttt
    78241 gcccactttc ggaaactgtt atatcgccat aatattcgag gtgatgatca gaagttactt
    78301 cttcgctttc tgcttatttg tttattcatt gctattctac ttctggtatc ttcttgttta
    78361 gcttggattt ttgaacatca cggcgcagaa ggaaattcta ttcaatcttt ctgggacggt
    78421 atttggtggg ccatagttac aatagccaca gttggttatg gagataaatt tccagtcact
    78481 tttcctggtc gcctggtagg tattatttta atcattgtcg gctattcatc attatcattc
    78541 tttactggca tggtggcttc actttttgtg gagaacaggc tgaaaggagc taaaggtttg
    78601 aaacagataa aaactcataa tcacatagtt atctgcggat ggaataaaac tgcagaattc
    78661 tttctgaaag cactggtaga aaaagaagta actgatctgg atatttgtat tgtagttaat
    78721 gatacgccgg acttttttga acgcctggaa tcatgctatc ctgctctttc tcttaagttt
    78781 gtgcgtggag aatcaattca ggaagaaacc ttgaaacgag cttctgtaga aacagcagct
    78841 cagataatta ttattgctga cgaacaattg gatcgttcat ctgttgatga tcacagcatc
    78901 attgtagcca atgctgttcg gtatcttgct aaaaaggata aaattacagt gcagcttata
    78961 aatccagaaa accgttccct gttggaacgg aatggtattc agaatattat tgtttgggat
    79021 gatattggtg gctatatttt agctaataat gtaactgata caaactatct gacagtaatc
    79081 tgtcagcttg cgcaagataa aaataatcat atcattacgc aaagaattcc tacggaattt
    79141 gtgagcaaaa gttttggaga actatccgat tattattata aggaatgtgg atatttgctg
    79201 ttaggtttat ttaccaaaga accagaattg gaattatctt ctattttcac ggaagataac
    79261 agtggtattg accaatttat ccaatatgcc ctagctaaaa gtcagaagat tataagggaa
    79321 gagaaaaaca atgtacgatg gaaaccgaac cgtgatattg aactgcaaac taatgattat
    79381 gctattttaa tggtgtagaa ggaaaattaa atgatagacc tggatacatt atctaaagtt
    79441 cccctattta ataatattac cattgaagct cttacatatt tgcagccatg ttttaaacag
    79501 atgttagttc ccaagggcac tgtaattata aaggaaaaca ctgagggcga tcagatttat
    79561 gttttggtaa agggttgtgt gcaagttacc aaagatctgg ttaaaggttt tgatacagat
    79621 caattttgga cggaaaaggt tttagcaaaa ttggaagggg attcatttcc cacttttgga
    79681 gagaatggtg ttttgggaaa tgggccacga actgcaaata ttatagctac aaccgattgt
    79741 gagctatata cattaacaaa gacagatttt gacagctttg ccaattatga cagtaaagct
    79801 gcttatatag ttatgaagaa cattgccatg aaattatctg aaagcttgaa actgacagat
    79861 gataatctgg taaaactggc aactgcactt tatatagctg ttcagcatta agtaaaacag
    79921 aaatttgctt atttgtaaac ttgcattaaa ttatagatga attataataa aattgtatct
    79981 ggaataaatg ataccgattt ccttaaaact atttaaggaa agacaataag aatttgaata
    80041 ctgggagata atgtggattt tactacaaac aaaataccaa gaaccttacc agttttgcat
    80101 atgagcaatg tagtaatgtt tccctacttg cttatgccac tggtagtatc ggatgaagaa
    80161 tctaagctcg taattgacta tgcattagcc aacgataaac taatggcttt tttcctggat
    80221 gaagaaaaag atgatactgg agaagttgaa cttgcacgag ttggaacagt agtaactatt
    80281 ttgcgtatgc tgcgtaatca ggatggttca ataagtatgt tacttcaggg atcaacccgc
    80341 gtcaaacttc aaaaaattgt ccagaaaaca ccttatttca tggttgatgt agaagcaata
    80401 cctgagcaaa tggacgaaga tacggaaatt caagcatatc gtactgtagc catagaatta
    80461 ctggaaaaaa tcgctcaaga aagcaatata ttaaatcggg aaatgattgc tggtcttaat
    80521 aacattaaac aagcaggaag agtagcagat attattgctg gaaatataga cctgccaata
    80581 aaagacagac agataatatt agagaccttt gatctaaaac agcggtttcg tcatctaaat
    80641 aactgtttgg ctgaattgat caaacagatg aaggtggaaa atcatataag aagtaatatc
    80701 caactggaaa tgaatgagga tcaacgtcga tattatttac gggaacaatt ggatgctatc
    80761 cgtagagagc taggtgaaac agatgaagta agcaaagaaa ttcttagatg gcgagaacta
    80821 attgcacaaa aaaatttacc tgattatgta gaagaagtag ctttagaaga attagaccgt
    80881 ctttctacta tgcaacctgc ttccagtgaa tatagcgtaa ttagaaacta tctggattgg
    80941 attgtaaatc ttccctggaa ggtttatagt aaagaccgtc ttaacctaat aaagattgaa
    81001 agtatcctgg aaaaggatca ttatggttta aaggaagcta aagaacgtat ccttgaattt
    81061 atagccgtaa aaaaattgaa agggaatcta aagggtccta ttctttgctt tgttggccct
    81121 ccaggaacag gaaaaacttc tattggtaga tcagttgcca gagcactttc tcgtaaattc
    81181 attcgtatgt ctctaggcgg aattcatgat gaagctgaaa ttcgtggaca tcgaagaact
    81241 tatattggag ctatgcctgg taaaataata atggaaatca aacgacaggg aacagctaat
    81301 cccgttttta tgttggatga aattgataag ctgggacggg attttagagg agatcctgct
    81361 tctgctcttt tggaagtgct tgatcctgaa cagaataaca gctttgtgga taactatata
    81421 aatcttcctt ttgatttatc cgaggttctg tttatcacta ctgccaattc tctggatact
    81481 attcctcctg cactgcgtga tagaatggaa ataatagaat tcactagcta tttggaaaac
    81541 gataaaattg agatagctag gcatttttta attccgcgtg aaaaagagaa taatggctta
    81601 gctaaggaga aaattacttt ttccaaatct gcactgcaag aaataatccg ttattatgtg
    81661 cgtgaagctg gagtgcgaaa tttacaaaga cgaattggtt ctatttttcg gaaaatagct
    81721 aaagaagtag caatgggaac aaaacaaaaa tggactatta aagctgatga tatagctaag
    81781 tatttaggtc cgcgtaaagt tactttggaa ttggctaatc gcaaaccaga gattggagta
    81841 gctacaggtc ttgcctggac aggttttggg ggagaaatac tattttgtga aacactccgg
    81901 atgccaggaa aaggaaatat tattttaac
//
LOCUS       DAOSTH010000004        68021 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_437_length_68021_cov_11.313907, whole genome shotgun sequence.
ACCESSION   DAOSTH010000004 DAOSTH010000000
VERSION     DAOSTH010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 68021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 68021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68021
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_437_length_68021_cov_11.313907"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..733
                     /locus_tag="PLB65_01585"
     CDS             137..733
                     /locus_tag="PLB65_01585"
                     /inference="COORDINATES: protein motif:HMM:NF019958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOQ77247.1"
                     /translation="MELTVRQKDILKAAIAIIANQGYEKLTIKNLATKIGVTEAALYR
                     HFKSKREIVTMILSYFEELSNRVLNEICESNNAPLDNIRKFVEDRYILFSKNPDLAKV
                     MFSEELFKNDPTFKGQFQCIMHKHKQAMESYLIQAQKDGNIKKDISSIQLFRIIIGSM
                     RFTVTQWNLSDGAFDLQKEGSDLFESIIKLIRIKEEDR"
     gene            760..1077
                     /locus_tag="PLB65_01590"
     CDS             760..1077
                     /locus_tag="PLB65_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOQ77248.1"
                     /translation="MQNRTWQEIEPKVNANGIKGTKIYEAPEGEIVHLYLAPGSHLKK
                     HITPVNVAFYVLEGTATLEIGEEKQSFPKDTLIESPKNIPHGVINEGNSVLRLLVIKM
                     PKP"
     gene            1093..2742
                     /gene="hcp"
                     /locus_tag="PLB65_01595"
                     /gene_synonym="priS"
     CDS             1093..2742
                     /gene="hcp"
                     /locus_tag="PLB65_01595"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HOQ77249.1"
                     /translation="MSMFCYQCQETARNLGCTVRGVCGKSETLANLFDLLIFGLKGLA
                     YASVHLQEKGRYYPEDSIFVMGALFKTITNTNWDEDSIRDTIEQTLALRDKRKKELCD
                     LMGGKCDTCPEAVTFQLKREEYDEFAKKVGVLKTENEDIRSLRETIIYGLKGICAYGE
                     HSAVLGYQDDEVNKFVMKALAATLDDNLTVDQLIQLVLKTGENAVKIMAKLDEANTKT
                     YGSPEPTKVKLGVNNNPGILVSGHDLKDLEELLQQTEGKGVDVYTHGEMLPAHYYPAF
                     KQYKHFIGNYGGSWWHQLEEFESFNGAILMTTNCLVPLRKENTYLDRLFTTGMVNYPN
                     AVHIPEREDGKAKDFTPIIERALKCQPPKEIETGEIIGGFAHDTVLGLADKIIEAVKS
                     GAIKRFVVMAGCDGRMPSRKYFTEVAEKLPKDAVILTAGCAKYRYIKLPLGDINGIPR
                     VLDAGQCNDSYSLAVIALKLKEAFGLDDVNKLPLSFDIAWYEQKAVAVLLALLYLGFK
                     NIKLGPTLPGFLSPNVANVLTETFGLHKTTDADSDIAAMFS"
     gene            3242..4264
                     /locus_tag="PLB65_01600"
     CDS             3242..4264
                     /locus_tag="PLB65_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77250.1"
                     /translation="MPKSYMIVIAIIAVLMLSSCAKRNTEYQEEDWLTLERALPIVGN
                     PLDIKIDDNYIYVAQDQGGISRINRSNYQVNWITEFWASDGSQKVLGRIKKIAVVPER
                     KFMFLVDTEATDAIRAIDISDDDSLIYVHDFTGGTGNIKDLDGFTIVDVPFAQGTYKM
                     VCAYCGSNSFRYDYYDGTIFNNNVFTVSPGIASGFKLTDNYIYVTAEQLGLYIYDRND
                     RHLVGKIHLNGEAQKVELCGNVAYVASRQSGLHTVDVYNPAAPVLLSTFDTSGYATSL
                     AISGNKLAVSSGGGGVYLFDISIPSQPKLIQHLTSCGYTNTVKFMDDRLVVGARDQGI
                     LIYKIK"
     gene            4362..9824
                     /locus_tag="PLB65_01605"
     CDS             4362..9824
                     /locus_tag="PLB65_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ77251.1"
                     /translation="MKATKVLTLLFVCMVNVLLAESVVSGERPLKVIAQNSGIITLQL
                     QVPELQIENTGTGEFKALNMDDAGFTAETGYPELPVFSTWIAIPPKGDIEVKVTSGDY
                     IIKKGVVPKPVYINENQEATLEYNRAAYRSSTLYPSSTYNYSQPQIIRDFRVVQISLN
                     PVQYLADTQELKIYKNMEVKIEITDKPGINEMDGYSGYSYAFTNIYKSMISNFEYYRD
                     ASMAPQSARILLIYGNNTDAVFLAKLNEFVVWKKQKGYEVNVASTVQTGGASNQAIHN
                     YIQTQYNDLATRPDYVILVGDTNGSYAIPTFTEQFSSYSGKGDYPYTFLAGNDYLGDV
                     FIGRISAENISQLDVLFNKIYAVEKNINVTGAAASWLNKMLLIGDPSHSGISTIYINK
                     FIKEMASRANPNYTFMENYSSGFSTYINSNINQGVGFFNYRGYIGMSGWDPSGSLING
                     SRLPHATILTCSTGNFENDTATTEQFIRLGTSVALAGAITAIGMSTSGTHTSFNNCLT
                     AGIYDGIFTYGMRSMGEALLNAKLYIKQVYGSTLDNYANYFSHWCNLMGDPTVEVFTQ
                     IPDQFNVICEDVLPLGSNILDVEVTNPLGEAVEGACVTLYSASQNLIIGKGFTDIDGN
                     ANIIISGGMLNELTVTVSKHNFKPMQKIVTIDPDGSLVYLNKIIIDNGSAGSTGNGDS
                     FASAGETIALNLEIKNTTLSTISATTATISSNDPYITIITNQSSFNEIESNGSLWSNS
                     AFVFSIHNNIPAQHNVRFNVQLTTAADDVYSFDLFVTGYNALLTVNNYTITSGTNNIL
                     DPGESAFLNITIKNNSVAPIYDVYAELVSLNDLVSVIDSDSYVGFISGNSLGSTMENM
                     EIFARPLLIAGMQIPMRLHLYNDSGFEQYADFNIPIGQVSSHTPLGPDTYGYFIYDST
                     DIAYVDCPSYEWIEINPSLGGAGTKITTFSDPGSSSDEGDQVGAVALQEINLPFAFSF
                     YGIEYDQITVCVNGFIVMGRTENAEFRNYHLPGGYGPAPMLAPFWDDLCLINDAGIYK
                     YYDSQNHLFIIEYDKMRNGYDRSTQETFQVIFYDPIYYPTSLGDGKVKFQYKDFNNVD
                     VGQGGYSPTHGNYCTIGIKDHTNTTGLEYSFNNQYANASAPITDSFALLITTAPVLHE
                     NPYLVLNELIIDDSNHNQIAEPGETIELGVKLMNIGLNPATQVQVSATCEDEFAELIN
                     NYTTYGNIQSDSIAVNRIPLSLHIDPNCPNGAEIIVNLHISISGFFWDYSVSIVVRKP
                     TLQICGIYMNDGIEGNGNGQVDPGETLDLIINIENGSPVSATNISSTLTSISQYVSIQ
                     NSTYIMSAIPAGDIKQSVYRLIISPDVFVGNNLTLYLNMLADQIDVQNEQIVLSVGTT
                     GMNANFETNNGNFVPNPANNSWEWGESIVAGAHSGTKVWGTRLNSDYPSYANYTLTTP
                     SVYIGSNFMLEFWHYYNCEANYDGGNVKISTNGGSTWNLLTPEGGYPASNLSVLNGPG
                     YCGNSNGWIIARFNLSNYANQNVQFRFTFASDTSINGPGWFIDDVRTTGYVEFASKVS
                     GTVSSSNSDIDYSNIEVRNSASLSTNPDSQGSYSIYLPLGTQSLWAEGKGYKNSEVAN
                     LVMASSTPWLQQDFYLGYLAPVIGLSYSVADSIFTLNWNPPVEPEYPLISYEIHQKIN
                     AGAFDTVAYSSEPIYTETMRELGTDYYYYIVCVYADGNSIPSDTLHYRYVVSGEDIVN
                     PVLVTKLLNNYPNPFNPETTIRFTLKETAPAKLYVYNIKGQLVKKLVDKVLPSGMHQI
                     VWNGKDNNNCNVASGMYFYRLESANYTCVKKMLLLK"
     gene            10275..11210
                     /gene="trxB"
                     /locus_tag="PLB65_01610"
     CDS             10275..11210
                     /gene="trxB"
                     /locus_tag="PLB65_01610"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOQ77252.1"
                     /translation="MMKYDVIIIGAGPAGLSAAIYSARSGLKTAVFERGLIGGQINVT
                     EEVENYPGFPEALSGYELTERMHRQAEHFGTEFKDEEVTALGMEGLCKIVETSDNKYR
                     ARAVIFCTGAYPRRLNVPGEEKFTGRGVSYCATCDGALYRDKIVAVIGGGDSAIEEGI
                     FLTHFAKKVVVIHRRDELRAQKIIQERAFKNPKMEFVWNSVVQEIRGENKVQELEVYN
                     RKTNTVSVIPVDGVFIYVGILPNNKLMESRIDLDSAGFALTDDFMHTNVPGVYAAGDI
                     RKKVLRQVVTATSDGAIAGWSAEKWITENYDAIQL"
     gene            11194..13824
                     /gene="alaS"
                     /locus_tag="PLB65_01615"
     CDS             11194..13824
                     /gene="alaS"
                     /locus_tag="PLB65_01615"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HOQ77253.1"
                     /translation="MPFSYNKGQKVLSSKEIRQQFIDFFLSKDHIFIPSSSVIPEDDP
                     TLLFANAGMNQFKSIFLGQKEITIKRAVNSQKCIRAGGKHNDLETVGKDGYHHTFFEM
                     LGNWSFGDYYKKEAISWAWELLTEIWNIPKDKLYATVYLTDTEAFELWKQETDIDPTH
                     ISYFEDKDNFWEMGETGPCGPCSEIHIDRGIEHCTMQNVQGHICQINGNCSRFIELWN
                     LVFIQYNREADNTLTPLKNKFVDTGAGFERLTQVLQDKNSNYETDLFMPIINRIEELS
                     GVQYTIEQGMPHRVIADHIRCLCFALSDGGFPSNEGRGYVLRRILRRAARYGRLLGFA
                     EPFLHLLVPIVIEQMAHHFRELSGKEDYIKMVIKAEEERFNRTLDTGLEKFNEITKKL
                     AGDIISGSDAFTLYDTYGFPLDLTAILAEEKGLKIDYQGFDKEMQEQKDRARKASKFT
                     LQTSNEDWIELAENHPTEFVGYTETKIKTNIQCYAVQEKGLILIQLAETPFYAESGGQ
                     VSDTGRIYNDGFEVLINNVRKQDDKIVHYGKLIRGTINSEPVTAEVSIERRKCTARNH
                     TATHLLHRALREVLGEHTVQKGSLVHPDYLRFDFAHFKALTFEEIRKVEDIVNNAILD
                     NRKVITTIQNIEEAKNEGAIALFGEKYNNIVRVVCVEDFSKELCGGTHISFTGEIGLF
                     KITSESSSAAGIRRIEAITGTTSLKWVQDLQDKLNHLATMLNTPIKKLENKLDATLEQ
                     INSLEKEIKELKNKEGEKLVLELLSKARTQENYSLIIEQTSFANPDELKQFAEQLKSN
                     MKSTIAVIFNLYQNKLNILCVVSSDLIPKYNAGKIVAILASELNGKGGGRADLAMAGG
                     KDTAKLSLVKEKVPELISSI"
     gene            13825..14811
                     /locus_tag="PLB65_01620"
     CDS             13825..14811
                     /locus_tag="PLB65_01620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HOQ77254.1"
                     /translation="MNILILRLSSMGDIILTQPICALLQKSYPGCNIDYLCKESFIEL
                     PEMFDPPVNPIVYQKTLKFHLWLFKSKYDIVIDLHNKFATYLIMLFIKASQKVHYCKK
                     RKFRQRIVKGDKKLKIDSTVSLYASALTKLGIKQPWSYPRLQISSLKLSNSIYPTNQD
                     CKDIKPIKIAVFPGANHFTKRYPVLSWIQLINSNPQYCFTLFGSTMDKDICDNIRKHC
                     PGNCINKCSIMSLSELYRELSTFQVIISGDTGPMHLAATLNKPQISIFGGTHPRLGFR
                     PLNDKAIVLCADLPCQPCSLHGRVKCPEGHFNCMNSINPALLSSAIKKALSS"
     gene            complement(14932..15795)
                     /locus_tag="PLB65_01625"
     CDS             complement(14932..15795)
                     /locus_tag="PLB65_01625"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HOQ77255.1"
                     /translation="MLFSCNSKPKTEPTIEKSEAEHPESNTEKIENQDQEKTTSSPDI
                     TKIKSYKYTWSKSDNMPPIAIIIDDFGQYSGKLLEDFTSLPKEIAFAILPDLPHTQTV
                     ARLADKTGHQVLIHIPMQAIDHKANPGKRFIKNGMDKFAIADLLQDFYAQIPNAIAAN
                     NHMGSEVTSDIATMSIVLDELNELGIFFLDSATTNKSTTYTAAENLGLITAKRDIFLD
                     VPDNSDKTIINKIEGLAKYKGRNEPIVIITHCHNREKLVALQKFLAQMDDMGVQLISV
                     SQLYKKLRAPA"
     gene            complement(15819..16310)
                     /locus_tag="PLB65_01630"
     CDS             complement(15819..16310)
                     /locus_tag="PLB65_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOQ77256.1"
                     /translation="MRKLILSVWLICVLCILFGCKRAEPQKKEPSSKIEFRQDGTLNI
                     SNPMGTVKATFSIEIVEKEEELMRGLKFRDQLGENQGMLFIFEYPDIYEFWMQDTYLP
                     LDMLFIASDGTINYIYENTVPFSEERITPNFTHKFVLEVNAGTVKKYNIQTGDKVTWK
                     RHS"
     gene            complement(16321..19206)
                     /locus_tag="PLB65_01635"
     CDS             complement(16321..19206)
                     /locus_tag="PLB65_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOQ77257.1"
                     /translation="MKKIALTFGLIIIAASIFAYPLRIQSWNLQADIKKLNELKVSID
                     YVNLQTGVIHIEVRNDSERDKITAAGIITELLPNPADEYFKGLKKGDLDGRYAYYTLT
                     EYQNFMQQIAAQYPNICQLVQFGSSVQNRPLLMMKISDNVMVDENEPELKYISSIHGD
                     EVVGYDMLIRLIQLLTSQYGIDPRITNIVNNTEIWINPMLNPDGYAAVSRYNANGIDL
                     NRNFPMPTGVQHPDSESWAVETIAVMDFSNAHDFDLSINFHGGALVVNYPWDYTYALT
                     PDNDLLTEMALTYARENLPMYNSTEFTHGITNGAAWYVITGSMQDWNYHYTDCIELTA
                     EIGTNKWPAASTLDSYWADNEEAILKYLEFAQRGVSGIVTNSLGTPIAATITVGGNSK
                     LEHTDLPVGDYHRLLLPGTYQITASANSFIPETVTVTVPQSGSVTQNFTLQPAMITNF
                     QGQLRNLQGNPVSGANIILNSDPQITVQTDTQGLFSFSIYEGDYQLSISAAGYTKIVQ
                     DIQIRQNDYRNIIIMQNPVFCEDFENGLGNWTATGNWGIMNYEGSNVLTDSPSGNYSN
                     MQNRSVTLTNPVSLSGIINPVLSFRCKYELESSYDYVHLEVSANSTNWTALDSFTGII
                     SSWVNKNYSLSNYTNGQVYLRFRIQSDWSQTADGIYIDDIAMTGINTNIPIYGDVTID
                     GRINIQDIAAINEYAIGLDPIIELDPRPWDTFRITNADVDNNGSIDSFDSYLLCKYIY
                     EPDYILPATSGIPEQVTIPIMNATYENNKLNLHFDDIDQLKSLVVSTAPNTINQVLHQ
                     GITFNHPFVQAVNNNDGSYGFAGYAIDQDSLYIILGTNPQNFTLYYTVNGVAGSQYVN
                     LSDAIDGSDLPKPFTYLFQNNPNPFNPETTLHFDLAENNTPVTLNIYNLKGQLIRRLV
                     SGVLSSGKHSVVWNGLDDRGKEVSSGVYLYRLNTPNYQQTRKMLLSK"
     gene            complement(19288..19386)
                     /gene="ffs"
                     /locus_tag="PLB65_01640"
     ncRNA           complement(19288..19386)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PLB65_01640"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(19421..21289)
                     /locus_tag="PLB65_01645"
     CDS             complement(19421..21289)
                     /locus_tag="PLB65_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77258.1"
                     /translation="MKKRILFFFLSLFLLCSPYWKAQTQGLDWTVYSSQQVLSIENAL
                     QYRFYPTAKTSLVINSKSNIENRLNFNQEAKNADLNVGLELMSKHFMHTISSDYQTFY
                     DNSDLEPAAYVNKTAILGYHINYSPVDSLNIGIFSKGILRNEQDRYVNGYSLSSEGYW
                     FGSNASYSNYILNSIAGISANAERKRMDWESFDYANLGANYNYYGNYLNFACLANYNY
                     HSEDIYVLAAPEQNEIRSNYQLSDNQLRWNFDISGNLQYSPEDRVQLEIRDNYSLRQT
                     SYSKSEIRNNTDHYNQAQLHLDYQISQKVSWQSDLAHTYAIKDYYYDLNTRQTDNRHI
                     GSRINWEYSEGDSLIAAYFVNLQIIKFPEDDNQWDNDLLTHNLRFGWKHYWHERVVLG
                     NWFGYSFSKDVYIDSLLSANNKEISSWTMFPECKFLIGDRIAFFQTYQIRADYSDYIY
                     NTGKNNTLYRQLGYQYNLVFDTFPLIARTLDPRWLELPFRNSPENAFMIDLGFAYEEN
                     QYAQQKQSYYELQTKNRRWTASIDFRHDIKNLYWTLSPQYTWGTWKELTFNLNSACLF
                     NNNSLIEFKLAPFIDNFENIDSVSFHNFSANKFFKMVKQCSEAADWRLSTTLRLYF"
     gene            complement(21240..22022)
                     /gene="rsmA"
                     /locus_tag="PLB65_01650"
     CDS             complement(21240..22022)
                     /gene="rsmA"
                     /locus_tag="PLB65_01650"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOQ77259.1"
                     /translation="MKALKALGQNFLIDKTIASEIVALGDLHKGDKVWEIGPGTGILT
                     DEILSWNVQLRAFELDKRLQKYLSEHYQGRFVLESTDILQANWDSLIQQDGYPLKLIA
                     NIPYQITSPLLAQLEKYSKFFSRIVLMVQKEVAQRLCAQSHSKHYSPLTIRLHLVYDI
                     YSKIQVNKEKFNPVPKVDSTVILLLPRTDKPIIKYPESFNNIIDAAFAHRRKTLANNF
                     IPVLGKEKTKELENLSQINFRQRGEELEEKDFILLSELVSSL"
     gene            complement(22086..22262)
                     /locus_tag="PLB65_01655"
     CDS             complement(22086..22262)
                     /locus_tag="PLB65_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77260.1"
                     /translation="MAKNNRGKGIKKLPNSGRGECPVCHRTGIKLLNEVKVGEKTYKV
                     CKTCKSVAAKKLSA"
     gene            complement(22582..23640)
                     /locus_tag="PLB65_01660"
     CDS             complement(22582..23640)
                     /locus_tag="PLB65_01660"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HOQ77261.1"
                     /translation="MNSENKKYLFDLLQTHSPSGFETPAQVLTNAYLSNYCEDITIDI
                     NGNLIAHKKGSGKHKIMIIGHADEIGFMVNYIDESGFIYVKALGGFDVNMLPGLRVDI
                     HHNDNIVHGIIGKKPIHLLRNSDEPSKLRLEDLWIDIGARNKEDAQNLVSIGDTITFS
                     SQIEELNEELIVSKATDNKVGVYIASAVMKELWEKDIKADYYAVSSVGEETTMRGAIT
                     SAYRIDPDIAVAVDVTFTSDIPGVDKHIYGDVSLGKGPVLCLGSALHPAINKKLTELA
                     KEHNIPLQIEISPSRTGTDADAVFASRQGTATAILSIPNRYMHTPNEIISLADLDNAV
                     QLLVEFVLSIDDTFELKR"
     gene            complement(23650..24045)
                     /locus_tag="PLB65_01665"
     CDS             complement(23650..24045)
                     /locus_tag="PLB65_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOQ77262.1"
                     /translation="MKVIHYDEAELEPVDAEGAKGAQIRWLIAQKDGAPTFAMRMFEI
                     EPGGHTPFHQHEWEHEVYCLSGNGAIVTERGEMAFSTDDAIFVEPNLTHTFKNVGKET
                     LKFLCLIPHEKPVIKKALNPFADEEANNC"
     gene            complement(24113..25219)
                     /gene="ribD"
                     /locus_tag="PLB65_01670"
     CDS             complement(24113..25219)
                     /gene="ribD"
                     /locus_tag="PLB65_01670"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020046386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOQ77263.1"
                     /translation="MTDFEINKYFNLAIQAAEKARGKCSPNPFVGSVIVKNNKVIAIG
                     WTLQYGGDHSEIQAIKHAGKEAEGATIFVTLEPCSHYGKTPPCAKAIIEAGIQEVYIG
                     IPDPNPIVSGNGIAMLKNAGIKVHCGFHEDKIKKQLEYYLCHIEKGRPFVTLKTALSL
                     DGKYAAGDGSSKWITGEKARKYVHKLRSENDVILTGIKTILADDPLLNARLPGNPKQP
                     LRVVLDPYLKIPVSSRFINSLSEFPGLIICSNSMLKTDKAKVLRQTGAVIHGLDCENG
                     HFNLSEVLNMLHNMNFYSILLETGSGLAEAFLEDNLIDKFIFIYGAKILGGTKSPFPN
                     LGLSNINQAILLSDISYRKIEQDLMVIAYPKVIT"
     gene            complement(25212..26390)
                     /locus_tag="PLB65_01675"
     CDS             complement(25212..26390)
                     /locus_tag="PLB65_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77264.1"
                     /translation="MLKLFKSNRCQSCYNERAMRQCPRKKKNICWQCCNETRCDTHCP
                     DSCPYSPKRDASNPFPAFKADSRAEAENVIKLHIDLWIGRENPMLQNITPEKMAVESP
                     QEMLSWLSGFQYPQYFPMDYLLQKLNLPFSPTSSKEDPETIAGKWMNSLIQLKWDNLR
                     QYTINESSLYDLAERYTEIIKSIPQFSKISTYSMLYSGLGEDGISAIVYMELNHKQDW
                     TLVLSNIKGDWKIRQNIAGNPEAYFKQNKLYSNIAEAIGKADDATAWDLIESNLKIYP
                     DCADLYYYRALYWQMVKQLDQATVDFFNSIALDNDWQEPYLHLSSHYLAKGDYEQAKV
                     WLLELQKMLPDDPNVLNNLAAAYAGNGELKYAKQLWQEILKKNPAFELARKNLEHLSD
                     "
     gene            complement(26391..27134)
                     /locus_tag="PLB65_01680"
     CDS             complement(26391..27134)
                     /locus_tag="PLB65_01680"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF010587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOQ77265.1"
                     /translation="MNYRKEIERIVAFIQNYCANTKFTNLLIGLSGGIDSALVAALAV
                     QAIGKKCVIAYNLPHRNSHPESAADALLMANHLDIPLQTIKIDDLVEKYFEHYAPDAS
                     SLRKGNWMARIRMNILYDQAAKHNALVIGTSNRSEYLVGYFTQFGDSACAFEPIAHLY
                     KTEVWEIAREIGLPDKIIQKTPTADLWAGQSDETEMGITYPVLDSILKYLTEGLPKPD
                     AEEKTIAKVKQMIEKSNFKRQMPPCLERI"
     assembly_gap    28778..28877
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(28963..29643)
                     /locus_tag="PLB65_01685"
     CDS             complement(28963..29643)
                     /locus_tag="PLB65_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ77266.1"
                     /translation="MLSPLDWVNEFMRKADSDIKEIRINEKEMEMDTKVNIPLSSLFI
                     KVGGNKMFHLFIKSIHQFYDTQEDVLKEVEFDMEKQESEGTKKIYCLAGLLYSTLQRG
                     GRLFIDEIENSLHPFLTKIIIKLFQDKETNPKGAQLIFTTQNTLFLNKDNFRRDEIWF
                     VEKAPNHSSNLYSLVEYKLPKGSTRKDASYAKDYIRGKYGAVPYVEYSEFVDLFKKSR
                     DKTKNEQN"
     gene            complement(29615..30283)
                     /locus_tag="PLB65_01690"
     CDS             complement(29615..30283)
                     /locus_tag="PLB65_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ77267.1"
                     /translation="MLVEFSVGNYRSIYQRVTLSMEAITKKELDDHTMEVSQQRLLKT
                     SVLYGANASGKSNIFKAFSFMKNMVLNSFKESQIGEPILVEPFLLNSTGKDEPSSWEV
                     IFLVDDVLYRYGFSAFRDHIAKEWLLMRKTTSRAREIVLFERLGQDIQVHNKFKEGKG
                     EIKDKLRENSLFISLLSQFNGFIAQKIVKCFFDSNLWYIDSYNPINTTALLDNNVVPL
                     RLGK"
     gene            complement(30481..31773)
                     /locus_tag="PLB65_01695"
     CDS             complement(30481..31773)
                     /locus_tag="PLB65_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HOQ77268.1"
                     /translation="MEEKFLYHIWDESHLATDLKTVSGKDLKIHYQGQFNTFRGPDFV
                     NAIISLDGEDLQGAVEIHQNTQDWLKHNHHEDVYYNQVILHIVLNHNSIQPYTMKENG
                     EFIEILELKNQLSEEIQKLITDIGDRKLSSGNDYCDLLSAIDNDRLFSILTLYGKQRF
                     MSKVRRFNASLAISDFDQVLYEGMMEAAGYDKNKFNLFQLAQSIPFIKLREWHKEGLM
                     SEEMISIFVGSSSLLQKSRNRISPELYEKLMCNYESQRFYARNLNVDWQLFRIRPGNH
                     PIFRLILLGEFLYNCLPAGLLNFFVSNVEALTPNPQKRYQNFCQQFQQKQRGLLQNSK
                     SLGSTVVNNIYINIYLPIIYLYYQKMANAEMLESVLESYLTFKALPENYITRFMCNHI
                     NSSQVKAVNNKTLYQQGLIDIYYRFCRYHLCSECVKNI"
     gene            complement(31773..32444)
                     /gene="mtgA"
                     /locus_tag="PLB65_01700"
     CDS             complement(31773..32444)
                     /gene="mtgA"
                     /locus_tag="PLB65_01700"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HOQ77269.1"
                     /translation="MRRRRPLLLRILRFIVILHLCYWGVIAFFSLLYNFVNPPLTPLM
                     VERYLVRGYPIHKRYYVPLEQIPKRTQNMLIAIEDGNFYKHYGFEWKMVKEAYQRNKK
                     AGRIRFGASTISNQLARTIFLTTRRSYLRKYLEIQATVIMEICMSKKRMLELYFNYVE
                     WGKGIYGIETASRFYYGKSCSKLSRNQTMKLISILTNPIRYSPQTYYKSASARQRYNL
                     LQRYF"
     gene            complement(32515..32775)
                     /gene="rpsT"
                     /locus_tag="PLB65_01705"
     CDS             complement(32515..32775)
                     /gene="rpsT"
                     /locus_tag="PLB65_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOQ77270.1"
                     /translation="MPQHKSPMKRMKTDKKRAARNNYVKSTIRTLSKKIHSDSTEEEK
                     EKILSELYSQLDKAAKKGVIHHRTASRRKARLADYVNKSKAE"
     gene            33036..33317
                     /locus_tag="PLB65_01710"
     CDS             33036..33317
                     /locus_tag="PLB65_01710"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ77271.1"
                     /translation="MKDDPKYQYMSQIMKAMAHPTRLFILDLLAEKEYCVCELQKQIG
                     TDISTVSKHLAVLKNAGLISSHKVNNQVWYNLLCPCVLDFYNCVSEIKN"
     gene            33338..33754
                     /locus_tag="PLB65_01715"
     CDS             33338..33754
                     /locus_tag="PLB65_01715"
                     /inference="COORDINATES: protein motif:HMM:NF020434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein"
                     /protein_id="HOQ77272.1"
                     /translation="MANNNTCSYSCNCNTGEGNDKCVTNLTKNKVYACGGASNVGMIS
                     FELAKTLHLENHYKMGCASGVAAGEFEVQNTIQQEGNQDLVIDGCPISCVQKIFEQKG
                     IEGFKHIIVTEFGINKEAAFNFEPATIKQLIDKILH"
     gene            33990..34682
                     /locus_tag="PLB65_01720"
     CDS             33990..34682
                     /locus_tag="PLB65_01720"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HOQ77273.1"
                     /translation="MDLRPKIGISINYMHLGDYMQYHIRDTYLDALFKNGALPILIPP
                     IEDKQVLMQYLDMVSFIIIIGGLDYPPFLYKEKQHPKTEPMEMHRAISDLYLLDLALK
                     MGKPVLGICAGMQLINIFFGGKLIQHLDNVDIHYGEKMHDIYVQKDSYWLSQIIPEGK
                     ILVNSNHHQGVDPTHIGKGLKVAAVSSDGCIEALEYNSEQMILGIQWHPERMQDTDHR
                     YRLFDFIIHFNQ"
     gene            34773..35963
                     /locus_tag="PLB65_01725"
     CDS             34773..35963
                     /locus_tag="PLB65_01725"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HOQ77274.1"
                     /translation="MAYGKIKNDLQKLFVELKEQGLYKNERILTTPQDAKIGVQGGTN
                     ALNFCANNYLGLGNNPEVIKAAQNIMNDWGYGCNSVRFICGTQAIHKQLEEAMSKFLG
                     MEDTILYAACFDANGGLFEPLLGEDSAIISDELNHASIIDGVRLCKAKRLRYKHSNME
                     ELESALKESQDCTYRIICTDGVFSMDGDMAKLDQICDLADKYDALVMVDDSHATGYIG
                     KTGRGTPEYFGVTDRVDIITTTFGKALGGANGGCTSGRKEIIELLRQRSRPYLFSNTL
                     APAIVGASLKVLDMLTHSSELRDRVWENASYFRKEMLDAGFDIVPGNTAIVPIMLYEA
                     PLAIKMADMLLEEGVYVIGFVYPVVPKGKARIRVQLSAAHTKEDLDFTIAAFKKIGSQ
                     LGLI"
     gene            36015..36878
                     /gene="rsmI"
                     /locus_tag="PLB65_01730"
     CDS             36015..36878
                     /gene="rsmI"
                     /locus_tag="PLB65_01730"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOQ77275.1"
                     /translation="MKYGDIYLIPVPIGNLGDITLRAIDLLKKAELIACEDTRVTSFL
                     LQQYKIPIPKLISFHKFNEKQREEFLLKYLESGEDLMVVSDAGSPAISDPAEKLVKAA
                     IDKGIKVFALPGASALLPAITVSGFSTRQFQFVGFLSQQPKSRREKLQEIAEYPYATI
                     IYEAPQRLHRLLRELLLHCGNRKICICREISKLYEEHIYTTLEEINQNPSLKIKGEFC
                     LVLDGKRINSNTDDESDKITEDKLIDYINEALDLGLKTKEISEKVSLMSSLNKREAYN
                     LVVETQKERDN"
     gene            36875..37564
                     /locus_tag="PLB65_01735"
     CDS             36875..37564
                     /locus_tag="PLB65_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOQ77276.1"
                     /translation="MIPFLLFDFDGTIADSIRLGIRIANNIAPKFGYEPFSEKDLKRF
                     QSLTWRNIISELHLPLYKIPKLISIGLSEYRHLISEVEPCEGILEMLNNLSERGIPMA
                     LLSSNTDENVKLFLDKYNIDTFKWVEGTAGTLHKAKHIKYQLRKHKLKPHKVIYVGDE
                     IRDIQAAHKCAMKIISVTWGFHSPEFLLHYNPDFLVNKPNEIVELIDTFIQDDLAIPF
                     NKINKKKEERA"
     gene            complement(37857..39713)
                     /locus_tag="PLB65_01740"
     CDS             complement(37857..39713)
                     /locus_tag="PLB65_01740"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOQ77277.1"
                     /translation="MKIRKAIIFLVVILIIVKIIFAFPFFKNLEYKAQDMLFRIRGKL
                     PTTDNIAIIAIDDASFSALDETWPFPRDYHSKLIENLNKAGVKQIIFDVEFTENSFPE
                     SDLRLAKTAAKYQNVIFAGKVLPSQHPGEPTQMIPPINAILDYNLPWGIVNMGAEPDG
                     FIRKYTLFEEFDQSKLYSLGIASLANLRVYQSTYADHIKVADRTLHLTDKSIPIIDKN
                     KALINFRGPSGTYPSVSYASVLDDSTFFMPGYQGAELNELYDIIESGILKDKIVLVGA
                     TIDELHDKFPTPYGGEWTPGVEIQANFIDMVLTDSYLKELNLWLYLLVEIILLIGLWF
                     LFKLLKPQFSAIALIGLLIIEFIVTYLLFSSSNIICPIAQTVVLLILIYVASILNHYL
                     DTLKEKRFIRNAFQQYLAPELVNQLLANPKNLKYGGSLQEITVLFSDIRNFTTYSEKH
                     SPEETVNILHQYFTEMVNIIIDNNGILDKFVGDEIMALYGTPIPLPNHALDACKSALK
                     MRERLIQLQEKWISEGYEPFEMGIGINTGMAVVGNLGSEQIFDYTAIGDTINLGARLE
                     SLNKEYDTFNHIIISEYTYEKVKDLVNAKYLDEVKVKGKNIAVKIYELISLS"
     gene            complement(39797..42196)
                     /locus_tag="PLB65_01745"
     CDS             complement(39797..42196)
                     /locus_tag="PLB65_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77278.1"
                     /translation="MFKHYLLLMLSMVLTVQIFAISLVHICPQTYTFNQKTDIKVEIQ
                     QGLSDIDKIKVIYRIDKQDHWFNEEMKQDSPGSVYFTATFPAKYLTTNVIEYYFVVEL
                     RQGNKEYFPPQNETVPNYILEPDIAYGEADPGFVLLTDNPIVAAEDGYLLVVSFFALA
                     DEIDPKTIEVWVGGKNVTANSQIIPPTIVYKEEKPASGIVRALVRAKKEKNIVHSQIW
                     STEVIPSGRISNKLTYQGSVNFASNYYDYSNKNAAGYTKSNASSWADFYANYGKFNAQ
                     ADIYLSSLEHSNSQPVNRYTIGVNSPHVDLFAGDYAPTFSQLTINGQNIRGIYTRLHT
                     ESVSFSVTQGETVRKTTSELDVDLFEDGIQKSGSFKQEALATRLQVGDENSFIFGLNF
                     SHHKDEVSSLDSVYYMYETTDDEGNKSINYTTKAQENAVIGIDAQLNIPEQNIVLGAE
                     IATSLLNTNIIPGALSKEEIEEYIGEDIPIDPQDLSDLFIINKNMIPFLPSRASIAWL
                     AYFRTYFWNNLFNVQYAETGSSFNSFGSTYQMRDSRVISFTDNLNISRYLILSGGINL
                     IEDNLMGHKSETNNTSSWYLQSILRVPKIPYLKLSYYNNLSDNEMNKDIDADFQKYEN
                     KSQSLICSIGYNITQIPYVPTQLDISYKIGSDNSYQIVEEENIDLTENDNNSLNLTML
                     NRFQKIPLTLKFAFSLNNNENLLYLTSKKSKTSNFFASASYSLWNDRINPYTDFRIIS
                     SSGAQNDRDYLYYSLGVEAYPLRSLSINTNIAITDYNNNNSNAYDYNNFIWRVLLTQR
                     F"
     gene            complement(42207..42881)
                     /locus_tag="PLB65_01750"
     CDS             complement(42207..42881)
                     /locus_tag="PLB65_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOQ77279.1"
                     /translation="MKRLFLISLALILAINIFAANSIAMISASKGKVNLRRAAKVVPH
                     KTGDFLYNKDEIRTGGESFAAYKYIDGSSTVKIFSNSIVYITATKNEKILDKKIELDK
                     GSVFTNIKPNTGSFSVATPTTVASVKGTAFFTKFDEFGQSTFIITEGEVELKILNKDT
                     QSITAGNTAVVQANGSFQIHNSTQEELQEIEQAELESTQGKENKKMRIPVIDPNGQTK
                     YIEITW"
     gene            complement(42896..44011)
                     /locus_tag="PLB65_01755"
     CDS             complement(42896..44011)
                     /locus_tag="PLB65_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77280.1"
                     /translation="MKRLIYIIVIIALLPVFANAQANFEVNFVPSSIRINSLSTASFD
                     PTQPDLQPILTTLMIKNLNTTPCRIDLKVALYWNNLLLANTIFCSREDFTLGYCTLNN
                     RDLITDEPMLNFEFKSGSTSISVEDILDASSTLKSAVMAGYFPDGELKFKVWVREFSE
                     TPWEGADNSPSAEFKIIIRNAGNITLLAPGRPIGQNPDKVSGLPISFLWNSQITGFND
                     YKLIIREYAPNNPPTINNVERTGSLFYETPSGKNECSGFSDFLPFTDSNYYAWKVTTA
                     LANEFNPIARGNKNNSLSSNWYIFQYVDEQEAAHSISEFQAHLDLLQNPTLINIHTQG
                     YLPVGVVVIEGKTYSGQEAINLIDALLGQDISVELKK"
     gene            complement(44008..44958)
                     /locus_tag="PLB65_01760"
     CDS             complement(44008..44958)
                     /locus_tag="PLB65_01760"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOQ77281.1"
                     /translation="MEKIVVLKGGTSPEREISLVSGSEVATALRTMGYIVSEIDPANY
                     PVLSNFITAIQNEHPLLVFNGLHGGSGENGELQATLSMAGIKYTGSNFKASCISMDKY
                     ISKLIAIAEGIPVPRYILLREDLLVDYQDPEDYSGFSNQLGLPIIVKPNDAGSSVGIS
                     RVEYLEDLKSAVQNAFKYSPNVLLEEYIPGRELTVTIIDGEAYPVVEIKPLEGWYDYT
                     NKYTSGKTHYEAPAQIDDTVAKLLQLYAIRLWKAFGLSGYARIDFRYDDLKPYFLEVN
                     TLPGMTSLSLTPMAAKAAGMSFQDLLKNIIDITIKYGGLK"
     gene            complement(45005..45910)
                     /locus_tag="PLB65_01765"
     CDS             complement(45005..45910)
                     /locus_tag="PLB65_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77282.1"
                     /translation="MIEIELTQPSFLCKSSLNVWNNPDFMQTVADISDLKPYHLVCYK
                     GESVVSCLPLYEKKVFGRKICKHPLLSYYQGLNLWLEDNSAPARRLLDALQITQTIAK
                     YLSCRYKVIRHILSPETYDVRGFLWNNMQAIPSYTFIHNFQNNIIPLSDERKKITLAE
                     KQDYTFEEEMNIEEFIHLMKLMNVKKRWQPGLSYQDLADFIRILHTAGILRQLNLKKD
                     GRIVSSNIILQDCAKAYTIFRATEIEALKKGASSLHTVKLIEFLKQENLEELDFCGAN
                     VSDIARFKAAMDLQLKVFFQIRTTL"
     gene            complement(45910..46266)
                     /locus_tag="PLB65_01770"
     CDS             complement(45910..46266)
                     /locus_tag="PLB65_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ77283.1"
                     /translation="MRPRILLVDDDRLLREVIGEYLSNHGYEVDLAEDADYALEKFEP
                     GKYQMAIIDYMMPGMDGLELMKILLERDPKIFCLIMTGYPTVDSAYQSIIDGASDYII
                     KPFHLKELLSTIGSYI"
     gene            46686..46898
                     /locus_tag="PLB65_01775"
     CDS             46686..46898
                     /locus_tag="PLB65_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HOQ77284.1"
                     /translation="MKGKVKWFNKNKGYGFIITEDNKEYFVHWKSIVTNSPRELKVLE
                     QDELVTFDLMETDKGIQAINIIRVNP"
     gene            46961..48613
                     /locus_tag="PLB65_01780"
     CDS             46961..48613
                     /locus_tag="PLB65_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOQ77285.1"
                     /translation="MPYLPINKSDLKQRGWNSLDIILISGDAYIDHPSFAAAIIGRTL
                     EAAGFRVGIIAQPDWENDSSFQVLGKPNLFFGITAGNMDSMVNHYTAQRKLRNDDAYT
                     PDGEIGKRPDRATIIYTNILKRLFKGVPIVIGGIEASLRRIAHYDFWQDKVRKSILAD
                     SKADILIYGMGEKPITELAMLLKKGKTIDEIQNLTSSVVFSATNPSPEDVVLPDAEIC
                     TDKNTFYQMTKIFEKNYETKVIYQKTAGRWLKHNLPAKPLTTEEMDAIYALPFEYKAH
                     PIYDGHKIPAYEQIKDSLTSHRGCYGGCNFCAIACHQGRRIQSRSENSLLKEAQKHKG
                     TISDVGGPTANMYASYCSLNFPDSCPRSSCLVPQICPNLRINHSLQLDVLEKITNLPN
                     IKYVFIASGIRYDMAVNDDKYIETIAIKYTGGRLKLAPEHSVPKVLRLMGKPNIDSFE
                     KFCKKFYSFTKKAGLKRKIVPYLIIGHPGTTMEDTYQLRNWLIRNKLVVEQVQEFTPT
                     PMSISTCMFYTGLDYYTGEPIHIPKPSEIRKQKNIIVAKYRH"
     assembly_gap    48946..49045
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            49278..51176
                     /gene="gyrB"
                     /locus_tag="PLB65_01785"
     CDS             49278..51176
                     /gene="gyrB"
                     /locus_tag="PLB65_01785"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOQ77286.1"
                     /translation="MQSSTYTASNIKVLKGLEAVRKRPAMYIGGTGERGLHHLVYEVI
                     DNSIDEAMAGFCDEIICTITNEGHIRVEDNGRGIPVDIQKDLGVPAVQVVLTVLHAGG
                     KFDDKSYKVSGGLHGVGVSVVNALSEFLEVNIHKEGKEYYMRFERGKPVTELQILGDS
                     KKSGTIVTFHPDPLIFETTEFSFDYLTTRLRELAFLNCGVRIILKDERSGRLHDFKYD
                     GGIKSFVEYLNQNKKPLTPNTIYLSNVKEGMEFEVALQYNEGYQENIFSFANNINTTE
                     GGTHLSGFKSGLTRAVNTYIKNADLLKNEKVSPSGEDIREGLTAVISIKIANPQFEGQ
                     TKTKLQNSEVEGIVNSAVYEKLLEYFEEHPAEAKNITMKSILAARSREAARKARELTR
                     RKSVLESGSLPGKLADCTLSDPAKTELFLVEGDSAGGSAKQGRDRSFQAILPLWGKML
                     NTEKARVDKVLNNDKIQPIILALGAGIGQDFDVSKLRYHKVIIMADADVDGSHIATLL
                     MTFFYRYMRPVIEEGKLYIAMPPLFLVRKGKQKVYVYNEEERDAAIKEFGEKGVIVQR
                     YKGLGEMNAEQLWETTLDPENRKMIAIKMDDAIEADRMFTILMGDEVEPRREFIQTNA
                     RYVKNLDI"
     gene            51190..53814
                     /gene="gyrA"
                     /locus_tag="PLB65_01790"
     CDS             51190..53814
                     /gene="gyrA"
                     /locus_tag="PLB65_01790"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOQ77287.1"
                     /translation="MADTTNIIPVQIEEQLKQAYLDYSMSVIVARALPDIRDGLKPSQ
                     RRIIYAMHELNLTPGGHFRKCAKIAGDTSGNYHPHGEQVVYPTLVRLAQPWIMRYPLI
                     DGQGNFGSIDGDPPAAMRYTEARLQKITMEMLSELEKDTVEFRSNYDETRQEPTVFPS
                     RFPNLLVNGSSGIAVGMATNMPPHNIGEVCEAIIALIDNPDLQPLDLLQYIKGPDFPT
                     GGFILGTEGIKDYFETGRGRIIMRGEAEIDTLPNDSEQIIIRSIPYQLSTTQLIDRMV
                     ELVKDKRIEGISDIRDESGRDGMRLVIQVKRNHDAQVVRNLLYKHTQLQATFGVINLC
                     LIDGVPQIVNMKEMLQNFIEFRHDVILRRTLFDLRNAKDRLHILEGFKIALDNLDAVI
                     ATIRASQTPADASSALQEKFGLTEIQAKAILDMRLQRLTGLERDKIEIEYQELLITIA
                     RLTELVEHKELRMNLIKEETSEIQDKFADKRLTTIIPAPAGSFNMEDLIADEMVVVTI
                     THDGYVKRLPVDTYKVQGRGGKGLSASNLKEEDFIQYIFVASTHSYILLFTDHGMCYW
                     LKVFEIPEASRTARGKAIVNLVNLAEGDRIKAFVTLKDFHPEQNIIMCTRKGLVKKTP
                     LSAFSHPRSNGIKAIKLREEDELIDARISESGDDILLATHEGKCNRFSESEIRLVRRF
                     SQGVRGIRLRNGDYVISMTIATAQDQLENGNPNAATILAISENGYGKRSPISSYSCTR
                     RGSMGVITLKTNKRNGYLASLMLTYDDDDLMIITQGGMIIRQKVSEISVVGRNTLGVK
                     LINLAPNDVVRDISLIHSEPDEESLDKEVETFKAKAPFKFEEKAATDEEDYEEEDEEM
                     DVIDELEEEDLDNEEDNE"
     gene            53825..54373
                     /locus_tag="PLB65_01795"
     CDS             53825..54373
                     /locus_tag="PLB65_01795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77288.1"
                     /translation="MILPAVILLLPVACSVNQFVPAEGVLSEDNYAIIRSDTLLIIVR
                     PQNYPGSYQEINSRFFPLYFKVKNTSSHKIHLNQSSFSILCNEKQYDSIPLDYVLADL
                     ERRILLKQFSDPFAPQVTNFGITYKEKEQEMYYELVNNAFSFGDILPGAIKEGYLFYN
                     REIASAKRFTIDILGNTICFVK"
     gene            complement(54395..55327)
                     /locus_tag="PLB65_01800"
     CDS             complement(54395..55327)
                     /locus_tag="PLB65_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77289.1"
                     /translation="MKRIFLSAICLLICLSAFAIDNKFLYQLAYVKSSQAYSIQFDND
                     LLYTNSHNYIWIYSIFNAWQPKLEAAYFSTFPIEDVETLAGKYIYVASSEPTNQVILV
                     DSLYTGSRIFFPQTILGEKLTREGATLYVADRYKGIDIINIGSGSTREILSTFSEKWG
                     IKDFVAAYPYIFALNDFGLVAVDISDQSYPVSLGVNYEIVDASCLVKYGNTIWIGAGK
                     NLMAFNVYDPKKPVLISQIRMTNEILNLAMKDNRLFIALGRGGVKIMDVTNPLKTEEL
                     NNIFLTVPVYDIDLANDYIFLGLGKEGWMIYEYR"
     gene            complement(55324..56310)
                     /locus_tag="PLB65_01805"
     CDS             complement(55324..56310)
                     /locus_tag="PLB65_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOQ77290.1"
                     /translation="MTKEEMIQDINSMQSLKDALLNEIHNVIIGQDRVLTDVLIALFA
                     NGHILLEGVPGLAKTLLISSLAKALSLSFSRIQFTPDLMPSDITGTDILVNDPITEQK
                     RFEYIKGPIFANIILADEINRTPPKTQAALLQAMQEYAVTSGTVTRPLSKPFLVMATQ
                     NPIEQEGTYPLPEAQLDRFMFFINIDYPTMEEELLIADSTTGLEIPTISPQLSGEQII
                     SFQQAVRSLPVSDHVLKYAVNLVRKSRPNTDEASKEIKQWVAWGAGPRASQYLILSAK
                     ARAALDGRYTPAEEDVKASAINVLQHRILPSFAAQAEGINSKQIIHWLLEER"
     gene            complement(56303..57307)
                     /locus_tag="PLB65_01810"
     CDS             complement(56303..57307)
                     /locus_tag="PLB65_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOQ77291.1"
                     /translation="MTHKNRNTLIIGLIVGIILLAAWLYYTPISEIRTQIAKVNYNWV
                     IFASLAYLFAYFLRSLRWRLLLPAQANPGICRTWLYSMAGNLLNYLIPIRAGDFARGW
                     FIKKNYDIPYAKALPSVFVDKVFDTLAILFIIIVLPFTTIELSTAMIILLCLLMLVFI
                     VAFSILVASVWQKEWVIKMLTFIFSWLPQKAKDRINPAIEMFIEELNIFEHQPLKLIL
                     ALLLTAFGILMDGTYFYLLFRAFGILYPFAITLFGYTLINLSYAIPQPPAQLGSNEWM
                     MIIIFSIGFGLTKSTASAIMAFGHILTAGLMSLWGIIAFAVLGPELFISVFKGEKIND
                     "
     gene            complement(57307..58143)
                     /locus_tag="PLB65_01815"
     CDS             complement(57307..58143)
                     /locus_tag="PLB65_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOQ77292.1"
                     /translation="MAVPIINDWAKYFDNPDEGLGSSYERVILNQLLDSICEVYHIQS
                     ALETPCFGFTGVSGINLVNLAKQGIKVSLEDHDLERITKINELWQELRLHIDIKYNPE
                     YVKLDYPDKHFDLGFNFSALWFTQNIRSFLAEFCRVCKKAILICVPNRDGIGFKMQMK
                     DYSPKKYPYLHPAHLDLATIKYLMKQNKWLLQKEGYIDCPPWPDIGMKKELFFKKLVG
                     KQEIQEKNREPLSILSYYQEENSDFISRMLSYGFVEKTAPPSFKKHWAHHYYLLFTPD
                     NG"
     gene            complement(58143..59126)
                     /locus_tag="PLB65_01820"
     CDS             complement(58143..59126)
                     /locus_tag="PLB65_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOQ77293.1"
                     /translation="MDRLLITGITGFIGRNVLRELMNTCPDYSITALVRPQTHPNRYS
                     EFGKDLRIIEIDLADTASLKEFLFTNEFDTILHIGALRGGRKFPKETYLRSNVYSTGQ
                     MVEYCLAKEARLIYCSSVGVFGAIPQELPAGPETEKNPDNYYHYTKIESERIINRAIL
                     NGLKASIIRPSIAYGEGDFGFPYQLVKLVDKYMFPLINRPIWIHLCHIDALVKAFVYL
                     ATNEWKSGLTLTIADREPVRLQALVDFISRQLHNKNYPGALTFDRIFFAWGEKLARML
                     KNELWISRFELISKSWFYDVTAYYDIMEREGIKPHFTIPEFEITIKDYQKH"
     gene            complement(59119..59907)
                     /locus_tag="PLB65_01825"
     CDS             complement(59119..59907)
                     /locus_tag="PLB65_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HOQ77294.1"
                     /translation="MVDIHTHLLPNIDDGSKSLDDSLSQISLMAEGGISRAYLTSHYF
                     RGHFQYTRSEYDDKFNLLVNEVIKAGIDIDLQPGFEIYLQPGILSDIKQHNLTMGKSN
                     YILLESDLNGLPSDFYNNIYPLLRAGYKPILAHAERYVSIMQKPSRAEDLIHRNIYIQ
                     TSAGSLLGLYGEKVKSTAWILVNKGWTHFIASDDHLRGKYYSYFEAKNKIEEVIDNHT
                     ANLLFYLYPSCIDNGDYIPYEYVEVHHSHHHQHHKHSLLKRIFG"
     gene            complement(59911..60792)
                     /locus_tag="PLB65_01830"
     CDS             complement(59911..60792)
                     /locus_tag="PLB65_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOQ77295.1"
                     /translation="MLLQKYFETRFEFIDQGLEQALSFEDEYAANLKKAMYEAVIPGG
                     KRWRPILLLSMFEMLTSFKKHRAFPDAVKAAVAIELMHNGGLIHDDLPCIMNRTQRRG
                     QPALHIKYGNAIAILAADALYSLAFEILGEFQNSEQAVKSIQILSNAAKSYGMIGGQV
                     VALSSRHKVMKINTLNYIDMKKVGSLLQASADIACLLSNADEETRQIMNTYAVNLGIA
                     YQMIEDIEADYSRGSDGLDDEYIPVSKSGYTGLLGFDKARKMVENMLEDTAKIIKPFP
                     NNSVLLEFVAMIKERLP"
     gene            complement(61096..62976)
                     /locus_tag="PLB65_01835"
     CDS             complement(61096..62976)
                     /locus_tag="PLB65_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ77296.1"
                     /translation="MQKYILLLLLLLSALSLFGTAKQDTLRTYNLPTVYVIVDKPSEA
                     IGSLHRIDSEDVKTSLSLREAMQNSVGISATTGSKDESNLRLRGFRKNEIKIMVDGRP
                     LNNGYFGNVDISKLSLLNIEEIQILKGPASPLYGTNNMGGVVNIITKAPPKNYWLELN
                     TIFKRNNTNEIKLSSAHSFDSWNYRIGLSREQTDGFNLSKDFIPTSSENGRIRNNSDK
                     CLYNLSGSWESDLLNFHNIGLDFGYSTMDKKNIPSSIYEHRYRQYQDWMRYYSGLWTK
                     FQMNESSVVNLQLTYDTSADRYLEYNDPTYETMTLDSQMRNQAFGVHPRLTKVFLGNR
                     KLDIGYHYEFQLNRRKDNGSYLEWTYSPVHLHQFFSQYECFLYPDLLLTTGLGLSAGI
                     NKYHNDFEPQLEPAVGITYDSSFNSKTSFAIGRNTSQPTLRQLYSESKGNPALKPQHS
                     LKVELSHNQALLKNKLSIYCSVYYNDTKDLIDMYLGRYENIFKVQSQGAEIGMDISLL
                     KFWQSSVNYAYLSYQENSDYYLTETPKNSVELIQRIKLPFNISFIFDSSFRDERLSQD
                     DEGRYHNLDPYWKHDLLITIPYKQIELSVGMENILDENYQCEYGFPEPGRNISIGFQI
                     KI"
     gene            complement(63023..63835)
                     /locus_tag="PLB65_01840"
     CDS             complement(63023..63835)
                     /locus_tag="PLB65_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77297.1"
                     /translation="MKKISVILIAILTLFMFACSEKDDNPTEVNVYGYKLDQFIFNTV
                     LRDLIDPNASDTLNFRNLFAYEIIASDGFSPRNSSYAGYDLTWDFFKEGFIVPSDNNR
                     TWFPSELGLPGAFKVKNTTKFNLYRKVDVNSAGKTNTVELKGLTIHSIQNWNSVNEDA
                     IKLSDLIQGITLSDSSNIRLISIDGYSKDYTPEQIAVGYYLMDSEVTTFTTLNELLPG
                     SLKKFKKLASIEVTNSTEQDNSFANAPHDKKDITITVPESFSGYDSTILTDY"
     regulatory      complement(63878..63999)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            64209..65114
                     /locus_tag="PLB65_01845"
     CDS             64209..65114
                     /locus_tag="PLB65_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77298.1"
                     /translation="MRKYPLIVILLLILVELFAFDIYFPDGKLLPINKEQLQQEKMVT
                     FNTLRDKDGAQKEENWQGIALIPWLNSLTNIPWHNLKIESKDSYSLTFHRLELNENNA
                     FIAMMQDGEELSEYNMRLIFTNYRENAWMRNIKCLRLLDYIPVPKPRQIFFWDQSFAK
                     NGIDFSSGYVPIGDLMLKCFFQDSGDIVFVDEQFRKLCLNYPRELGSVFLHIDEKGNY
                     TLAPDKSSHLKISSQTDLSQLIYVQCGQVAYIKENYVQEIRQMAKVLNWDWESDSLHL
                     IKASKEEIKAGDKPKEINADCWLEF"
     assembly_gap    65147..65246
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<65247..66574)
                     /locus_tag="PLB65_01850"
     CDS             complement(<65247..66574)
                     /locus_tag="PLB65_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOQ77299.1"
                     /translation="MKRGLYILLLLLSACSVGKKQPEWTLQQPFDNGYYSAVVKIPKK
                     APNYKELARNEAIREISTQISVQIDSDIALKETEANGIPSSELISSIRSSSNNKIRDL
                     QLVGTYETKNDYWAYYRLSKSEYYAWRKNQCEQATAQALNLLNEFDLSQTNLTSGISA
                     LLKGLELIVDYTDMDLTTSYRNKQINLYNELFFRLNHLTEKVKINYAEKEIDLTAKQR
                     ERKSIAVFVNYQQEIPVNNFPVCFNFLSGKGEIVAEGLTDENGKAELIINRITSFSTP
                     QFIEAKPDKDFWLVGIENPIVKTMFGNLQFLPATLKLNVNRPKAFVQYSFNNTSGTDY
                     RNILLKKLQDLDLEISTNQNESDYTFEVNIITRNSEFLPLLKQYSAVADAYIELIDSR
                     TGKSIYNTNLTGIKSVAVTTDAAKGKNELDAVNELCDKVMFMLVQQYIMY"
     gene            complement(66574..67842)
                     /locus_tag="PLB65_01855"
     CDS             complement(66574..67842)
                     /locus_tag="PLB65_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HOQ77300.1"
                     /translation="MKKWLFPVLLCCLLIISACSSNARLAKKVQKNYEKGNYEIATQE
                     AIRALSKKADNPKAQELLVQSWQNYRLAQQRKIEKLMQSNETNKWEQIYQEYTSLQNL
                     AEQIQSLPPLINPYTGYKVVIDIPDYAAEMKQSQENAAEAHYQAGILYAKMSNDKDTQ
                     KKAALEFKEALKLIPNYRDADLRYEQSRKLAIKRIAITPFEDKSNTSGIYGAVSDILT
                     DQIVSLLISSAAKSDFVEIIDRTQLEAVMKEQQLTASGLINNEGSFRLGQILGADEIL
                     TGRILQINVTPERTVSITSEDETEVVIRTEEYTDEDGITQEREIKGKVFCRYRKFTKT
                     ANVSISTSYSILEVETGKIKQQETVEIKNSWSDTWARKISGDDRALSYNIKKLMEKAE
                     PFPPSINEMVKEALKQTGTDIANKLCGYLS"
BASE COUNT        21910 a        11645 c        12184 g        21982 t
ORIGIN      
        1 tcatatcata ttgttgtaca gacacatatt ggcgtctatc ctcctctttt ttcatatcct
       61 actaaaaaag ttcttgacaa gcaagcgaaa tcaattatat tggtatgtga atacttacta
      121 acataaggaa atttaaatgg aacttacagt caggcagaaa gatatcttga aagcagccat
      181 agccattatt gcgaatcaag gttatgaaaa actaaccatc aaaaatttag caactaaaat
      241 cggagtaact gaagctgctt tatacaggca ttttaagagt aaacgagaaa tcgtaacaat
      301 gattttgagt tattttgagg aattatccaa tcgtgttctg aatgaaattt gtgaatcaaa
      361 caatgctcct ctggacaaca ttagaaaatt tgtagaagac agatatattc tttttagcaa
      421 aaatcctgat ttggctaaag ttatgttttc ggaggaatta tttaagaatg acccaacttt
      481 taaaggtcag tttcagtgta ttatgcataa acataaacag gcaatggaaa gttatcttat
      541 tcaggcacaa aaagatggaa acataaaaaa agatatatcg tctattcagt tatttagaat
      601 cattattggt tctatgaggt ttaccgtaac tcagtggaat ttatctgatg gtgcttttga
      661 ccttcaaaaa gagggctctg atttatttga gtccataata aaactaataa gaataaaaga
      721 agaagataga tagcaggata aaaaaataag gaggagaaaa tgcaaaaccg aacttggcag
      781 gaaatagaac ccaaagtaaa tgccaatggc attaagggaa cgaaaattta tgaggcgccc
      841 gaaggagaaa tagttcatct atacttagct cctgggtcac atttgaagaa acatatcact
      901 ccagttaatg tagcttttta tgttctggaa ggaacagcta ctttggaaat tggagaagaa
      961 aaacaaagtt ttcctaagga tactttaatt gaaagtccga aaaatattcc ccacggtgtt
     1021 attaacgaag ggaatagtgt cttaagatta ctcgtgataa aaatgccaaa accataaaaa
     1081 ggagaaaatg aaatgagtat gttttgttat caatgtcaag aaaccgcacg caatcttggt
     1141 tgcactgttc gcggcgtatg tggaaaaagt gaaactcttg ccaatttgtt tgatctctta
     1201 atatttggac taaagggtct tgcttatgcc tcagttcacc ttcaagaaaa aggtagatat
     1261 tatcctgaag attctatttt tgtgatgggt gccctattta aaactataac taacacaaat
     1321 tgggatgaag atagtattcg ggacacaatt gaacagacat tagctttgcg cgataaacgc
     1381 aaaaaagagc tatgtgattt gatgggcgga aaatgtgata cctgtccaga agcggtaacc
     1441 tttcagctaa aaagagaaga atatgatgaa tttgccaaaa aagttggcgt gctgaaaact
     1501 gagaatgagg atatccgttc tttgcgggaa acaattattt acggactgaa aggaatttgt
     1561 gcttatggag aacattctgc tgttttgggt tatcaggatg atgaggtgaa taaattcgtt
     1621 atgaaagctt tggcagcaac tttggatgat aatttaactg ttgatcagct aatccaatta
     1681 gttttgaaga caggcgaaaa tgctgttaaa ataatggcta aattggatga agcaaatacg
     1741 aaaacctatg gtagtcctga gcccaccaaa gttaagctgg gtgtaaataa taaccctggt
     1801 attttggtta gtggtcatga tctgaaagac ctggaagaat tgttacaaca aactgaagga
     1861 aagggtgtgg atgtttacac tcacggagaa atgcttccag ctcattatta tccagcattt
     1921 aaacaataca aacattttat tggcaactat ggaggttctt ggtggcatca attggaggaa
     1981 tttgaaagtt tcaacggagc cattttaatg actactaact gcctggtgcc tttacgcaaa
     2041 gaaaacactt atctggatag attgtttaca accggtatgg taaattatcc aaatgccgtt
     2101 cacatccccg aaagagaaga tggcaaagca aaagatttta cgcctataat tgaacgagct
     2161 cttaaatgcc aacctccgaa agaaattgaa accggtgaaa ttattggtgg ttttgctcac
     2221 gacacagttt taggactggc agataaaatt attgaagcgg ttaaaagtgg agccataaaa
     2281 cgctttgtag ttatggctgg atgcgatgga agaatgccca gccgtaaata ttttaccgaa
     2341 gtggcggaaa aacttcctaa agatgctgtt atcctaactg ccggatgtgc caaatatcgt
     2401 tatataaaac ttcctttggg tgatattaac ggaattccaa gagttttgga tgctggacag
     2461 tgtaatgatt cctattcttt ggcagttatc gcactgaaac taaaagaagc atttggactg
     2521 gatgatgtaa ataaacttcc tctctcattt gatattgctt ggtatgaaca aaaagctgtg
     2581 gcagtgcttt tagcgctatt atatttagga ttcaaaaata ttaaattggg tccaacacta
     2641 cctggttttc tttctcccaa tgtagcaaat gtactaactg agacatttgg tttacataaa
     2701 accactgatg ctgacagtga tattgcagca atgttttcat agaaatctca tatcctaaaa
     2761 ataagctccc tgctatagag tagggagctt atttgtttta agtaattgtg atggttatta
     2821 atcttataat ccatatgttc attttaactt aaaagtgaga cattatggtt atgtccacta
     2881 taaagcataa ccagatctca attttattcg tattatcgtt atgcatacta tctatgttta
     2941 tgaaaaaatg aatacaaaga tccaaataac acaatattta ataggtgggg accaagattg
     3001 attttgtcac cctctggggc cgtttcacaa taaataaaac tcctttttta gccgattttg
     3061 gatatataaa atgtactctc agagaagacc atttaataca atggcttata tgaactgtct
     3121 tacaacagta ttttgtttcg gggcagctcc atatagaaat caataaaaat gagcaaatct
     3181 actatttcgg aacgcttctt gctttattta tagacatact aacaggcagg ggaggaaatt
     3241 gatgcctaag agttatatga tagtaatagc gattattgca gtgctaatgc taagttcctg
     3301 tgcaaaacgc aacacggaat accaggaaga agattggctt acccttgaac gtgctctacc
     3361 tattgttggc aacccattgg atatcaagat tgacgataat tatatatatg tagctcagga
     3421 tcaaggtgga atttccagaa tcaaccgttc taattatcaa gtaaattgga taactgaatt
     3481 ttgggcttca gatggcagcc aaaaggtttt aggtaggatc aaaaaaattg ctgttgtacc
     3541 cgaacgtaag tttatgttct tagtggatac agaagctaca gatgctatca gggcgattga
     3601 tatcagtgat gatgattccc ttatctatgt tcatgatttt acagggggca ctggtaatat
     3661 aaaagacctg gatggcttta ctattgttga tgttccattt gctcagggaa catataaaat
     3721 ggtttgtgct tattgtggtt caaatagctt tagatatgat tattatgacg gaacgatctt
     3781 caataataat gtttttacag tttctcctgg aattgcatcg ggttttaaac tgactgataa
     3841 ttacatttat gtaactgcag aacaacttgg tttatacatt tatgatcgaa acgaccgtca
     3901 tctggtagga aaaattcatt taaacggaga agcacagaaa gttgagctct gtggtaatgt
     3961 tgcttatgta gcaagccgtc aaagtggttt acatactgtg gatgtttata atcctgcagc
     4021 tcctgtattg ctttctacat ttgatacatc tggttatgca acttctttgg ctatttcagg
     4081 aaacaaactt gccgtatcct ctggtggcgg aggagtatat ttatttgata tcagcattcc
     4141 ttctcagccg aaattgattc aacatttaac ctcttgcgga tataccaata ctgttaaatt
     4201 tatggatgat aggttggtcg ttggagcaag ggatcaggga atacttatat ataagataaa
     4261 atagtttatt aaggcgacta aacaatgatt ttggagaacc tgaagttaaa aaagctaact
     4321 tcgggttctc ttactttaaa taaagaaagg aataaaaatg aatgaaagca actaaagtat
     4381 taacactatt attcgtatgt atggtcaatg tactattggc ggaatcggtt gtttctggtg
     4441 aacgaccgtt gaaagtgata gctcaaaatt ctggcatcat aacactccaa ttgcaggttc
     4501 cagaactaca gattgaaaat acaggaacag gtgaatttaa agcactaaat atggatgatg
     4561 cgggtttcac agcagaaact ggctaccctg aactaccagt tttttccact tggattgcaa
     4621 ttcctccaaa aggggatata gaagttaaag tgaccagtgg agattacata atcaaaaaag
     4681 gtgtagttcc taaacctgtt tacattaatg aaaaccaaga agctacattg gaatataata
     4741 gagctgctta tcgcagttct actttatatc cttcaagcac ttataattat agtcaacccc
     4801 agattattcg cgattttagg gttgtgcaga tttcattaaa tcctgtccaa tatttggcag
     4861 acacgcagga actgaaaata tataaaaata tggaagtgaa aattgagata acagataaac
     4921 cgggaatcaa tgaaatggat ggttacagtg gttattcata tgccttcacc aatatttata
     4981 aatctatgat aagtaatttt gaatattatc gagatgcttc aatggcacca caatcagcca
     5041 ggatcttatt gatttacggt aataatactg atgctgtctt tcttgctaag ctgaatgaat
     5101 ttgtcgtctg gaaaaagcaa aagggatatg aagttaatgt tgcaagtaca gtccaaactg
     5161 gtggtgcatc caatcaagct attcacaatt atattcagac gcaatataat gatcttgcta
     5221 ctcgtcctga ttatgttatt cttgtaggtg atactaacgg gagttatgct attcctactt
     5281 ttacggaaca attttcctct tactctggta aaggagatta tccatatact tttttagctg
     5341 gtaacgatta tctgggagat gttttcattg ggagaatttc agctgaaaat atcagccaac
     5401 tagatgtact atttaataaa atttatgcag tagagaagaa tattaatgta actggagcag
     5461 cagcttcttg gttgaataaa atgttgttga ttggtgatcc cagtcacagt ggtatttcca
     5521 ccatatatat aaacaaattc atcaaggaaa tggcaagcag ggcaaatcct aattatactt
     5581 tcatggagaa ttattccagt ggtttttcta cttatattaa tagcaatatt aatcagggag
     5641 taggtttttt caattatcga gggtatattg gaatgagcgg ttgggatcct tccgggagtt
     5701 taataaacgg aagcaggcta ccgcatgcta caattttaac ctgcagtact ggaaattttg
     5761 aaaatgacac tgctacaact gaacaattta ttcgcttagg aacttccgta gctttggcgg
     5821 gagcaattac tgctattgga atgtcgacca gtggaactca tacttctttt aataattgtc
     5881 ttactgcggg aatttatgac ggcatattta cttatggtat gcgcagtatg ggtgaagcat
     5941 tacttaatgc aaagctctat attaaacaag tttatggttc tactttagat aattacgcaa
     6001 actatttttc ccattggtgt aatctaatgg gtgatccaac agtggaggtc tttacacaaa
     6061 taccagatca atttaatgta atttgtgaag atgttcttcc tttgggtagc aatattttgg
     6121 atgtagaagt tacgaatcct ttgggagaag ctgtagaagg ggcatgtgtt acattatata
     6181 gtgcatctca aaatttaata atcgggaaag gattcactga tatagatggc aatgctaata
     6241 taattatatc gggtggaatg ttaaacgaat taacagttac agtttccaaa cataatttca
     6301 aaccaatgca aaagattgta actatcgatc cagatggttc tttggtctat ttaaacaaaa
     6361 ttataattga taatggtagt gctggaagca ctggaaatgg cgatagtttt gcctcagcgg
     6421 gagaaacaat agccttaaat ctagagatta agaacactac tttatctact atttctgcta
     6481 ctacagcaac catatcatct aatgatccat atataacaat tattactaat caatccagtt
     6541 ttaacgagat tgagagcaat ggctctcttt ggtcaaattc tgcttttgtg tttagcatcc
     6601 ataataatat acctgctcaa cacaatgtgc gatttaatgt tcaattgacc actgcagctg
     6661 atgatgtata ctcttttgat ctctttgtta ccggttataa tgctttattg actgttaaca
     6721 attatacaat tacatcagga accaacaata ttcttgatcc gggtgaaagt gctttcctga
     6781 atataacaat aaagaacaat tctgttgctc cgatttatga tgtttacgcg gagttagtgt
     6841 cgttgaacga tttggtctcg gttatagatt ctgattccta tgttggtttt atttcaggaa
     6901 atagtttggg aagcactatg gaaaatatgg aaatatttgc cagaccttta cttatcgctg
     6961 ggatgcaaat acctatgcgt ttgcatttat ataatgacag tggttttgag caatatgctg
     7021 attttaatat tcctatcggt caagttagtt ctcatactcc tttaggtcca gatacttatg
     7081 gctattttat ctatgattca actgatatcg cttatgttga ttgccccagt tatgaatgga
     7141 ttgaaataaa tccaagttta ggaggagcgg gaaccaagat tactactttt tcagatcctg
     7201 gaagttccag tgatgaaggt gatcaagtag gagcagttgc tctacaggaa ataaatttac
     7261 cttttgcctt tagtttttat ggaattgaat atgatcaaat tactgtttgt gtaaatggct
     7321 ttattgtgat gggtagaact gaaaatgcag aatttcgtaa ttatcatctt cccggtggat
     7381 atgggcctgc accaatgctt gcaccttttt gggatgatct gtgtttgatt aatgatgctg
     7441 gtatatataa atattatgat agccaaaatc atctatttat aattgaatat gacaagatgc
     7501 gtaatggata cgatcgctcc actcaagaaa cattccaagt gatattttat gatccaatat
     7561 actatccaac ctcgttagga gatggaaaag taaaatttca atataaggat tttaataatg
     7621 tagatgttgg ccagggaggt tatagtccta cgcatgggaa ttattgtaca ataggaatca
     7681 aagatcatac aaatactact gggctagaat attcttttaa caatcaatat gctaatgcat
     7741 ctgctcccat aacggattct tttgctttac ttattacaac agctccagtg cttcatgaaa
     7801 atccatatct cgtacttaat gagttaatta ttgatgattc caaccataat cagattgcag
     7861 aaccaggaga aaccatagag ttgggagtaa agttaatgaa tattggttta aatccagcaa
     7921 cccaggttca ggtatctgca acctgtgaag atgaatttgc agaactgatt aataattata
     7981 ctacctatgg taatattcag agtgattcta tagctgtaaa tcgaattcca ctcagtctac
     8041 atatagatcc aaattgtccc aatggagctg agattattgt aaatttgcat atcagtattt
     8101 caggtttctt ctgggattat tctgtatcca tagttgttcg taaacctact ctccaaatat
     8161 gcggtatata tatgaatgac ggtatagagg gtaacggcaa tggtcaagta gatccaggag
     8221 aaacacttga tttaattatc aatattgaaa atggttcgcc agtttcagct accaacatat
     8281 ccagtacttt aacctctatt tcacaatatg tatctatcca aaacagtacc tatattatgt
     8341 ctgcaatacc tgctggagac ataaagcaat cagtctatcg gctgattatt tcgccggatg
     8401 tttttgttgg caataatcta actttatact taaatatgct agcagatcag attgatgtac
     8461 agaatgaaca aatagtttta agcgtaggca ctacaggaat gaatgctaat tttgaaacca
     8521 ataatggaaa ctttgttccc aatcctgcca acaacagttg ggaatggggt gaatcaattg
     8581 ttgcaggagc acattctggt acgaaagtat ggggaacgcg tttaaatagt gattatccat
     8641 cttatgctaa ttatactctt acaactccct cagtgtatat cggctccaat tttatgcttg
     8701 aattctggca ctattataat tgcgaagcaa attacgatgg agggaatgtt aaaatatcaa
     8761 ctaatggagg ctcaacctgg aatttactta ctcctgaggg tggatatcct gctagtaatt
     8821 taagcgtttt aaacggacct ggttattgtg gaaacagcaa tggatggata atagcacgct
     8881 ttaacttgtc aaattatgct aatcagaatg tccaattccg ttttactttt gccagtgata
     8941 cttctattaa tggacctggt tggtttattg atgatgtgcg gactactgga tatgttgaat
     9001 ttgccagtaa ggtttcggga accgttagta gtagcaattc tgatatcgat tatagcaaca
     9061 ttgaagttcg aaattctgct tctttgtcta ccaatcctga ttcccagggt agttattcca
     9121 tttatctgcc tctgggcaca caatctttat gggcagaagg taaaggatat aaaaattccg
     9181 aggttgctaa tctggttatg gctagtagta ctccttggct tcaacaggat ttctatctgg
     9241 gatatttagc tccggttata gggctttcgt acagtgtggc agatagtata tttaccttaa
     9301 attggaatcc acccgtagaa cctgaatatc cactaataag ctatgaaatt catcagaaaa
     9361 taaatgcagg tgcttttgat acagtagcat attcttctga accaatctac acagaaacga
     9421 tgagagaatt ggggacagac tattattact atattgtttg cgtttatgct gatggtaata
     9481 gtataccatc agatacttta cactatagat atgtagtaag tggagaagat atagttaatc
     9541 ctgttctggt aaccaaattg ctgaataatt atcccaatcc cttcaatcca gaaacaacaa
     9601 tcagatttac tctgaaggaa acagctcctg ctaaactcta tgtatataat attaagggtc
     9661 aattggttaa aaaacttgtg gataaggttc tcccttcagg aatgcatcaa attgtctgga
     9721 atgggaaaga taataataat tgtaatgttg cctcaggaat gtatttctat agattggaaa
     9781 gtgctaatta tacctgtgtg aaaaaaatgc tgcttttaaa ataaacatcc ttcaataagt
     9841 caggagtcta aaagtctttt gataactctg gtttttacac caatgactta taaaaccttt
     9901 aggactcctg acttctaact actattatag agtgaaattc tacaatattt aaggtcaacg
     9961 atcatcagta ttgcctataa tttagtagtt gcttcattac tggtgtttaa gagaacaact
    10021 gagttaaact gatgcttatg aataaatact actcctatta gcgaggaaaa cggtatcagt
    10081 aaaagcttat gattctttaa ctctctcata ctgctactcg ggagggttac aatattaatt
    10141 acaataaaat tatttgtaag atacccaaaa tgctttgtgg aagaagacaa gagaaaactg
    10201 attatcaatt ttacttgacg aatcagctaa cttaaagctg tttgcaatca agattcaaaa
    10261 aaggtgaaga acgaatgatg aaatatgatg taattatcat tggggctggt cctgccggtt
    10321 taagtgcagc catttacagt gcgcgaagcg gattaaaaac agcagttttc gaaagaggct
    10381 tgattggcgg tcagataaat gttacagaag aagttgaaaa ttatcctgga tttccagaag
    10441 ctctaagtgg ttatgagctt acagaaagaa tgcatcgtca agcagagcat tttggaacag
    10501 aatttaaaga tgaagaagtt acagctctgg gaatggaagg tctttgtaaa atagtggaaa
    10561 cttcagataa caaatatcgt gccagagccg ttatattttg cacaggagct tatccgcgtc
    10621 gtctaaatgt tcccggtgaa gaaaaattta caggccgtgg agtttcttat tgtgctactt
    10681 gcgatggagc tttatataga gataaaattg tagctgttat tggagggggt gattctgcta
    10741 ttgaagaagg tattttcctc actcatttcg ccaaaaaagt tgtagtgatc cacagacgag
    10801 atgaacttcg tgctcaaaaa atcattcaag aacgagcatt taaaaatccc aagatggagt
    10861 ttgtctggaa tagcgttgtt caagagattc gaggagagaa taaagtgcaa gaattagaag
    10921 tatacaatcg gaaaaccaat actgtaagtg ttattcctgt tgacggggtc tttatctacg
    10981 taggtatttt accaaataac aagttaatgg aatctcgcat tgatcttgat agtgcaggtt
    11041 ttgctctaac tgatgatttt atgcatacga atgttccggg agtttatgct gctggagata
    11101 ttcgtaagaa agtactacgc caagttgtaa cagcgaccag tgatggagcc atagctggtt
    11161 ggagtgctga aaaatggata actgaaaatt atgatgccat tcagttataa taaaggacag
    11221 aaagtgctaa gcagtaaaga aattcgccaa caattcattg attttttcct tagtaaagat
    11281 catattttta ttccttcatc atctgtaatt ccggaagatg atccaaccct cctttttgcc
    11341 aatgcaggaa tgaatcaatt caagagtatt ttcttaggac agaaggaaat aaccatcaaa
    11401 agggctgtta atagccaaaa gtgtattcgt gctggtggaa aacataacga cctggaaaca
    11461 gtaggaaaag atggttacca tcacactttt tttgaaatgc ttggtaactg gagttttggg
    11521 gattactata aaaaagaagc tatttcttgg gcatgggagc tgctaactga gatttggaat
    11581 attcccaaag ataagctcta tgctactgtt tatttaacag atacagaagc ttttgaacta
    11641 tggaaacagg aaacggatat tgacccaact catataagct attttgaaga taaggataat
    11701 ttttgggaaa tgggggagac aggtccttgc ggtccctgtt cggaaattca cattgaccgg
    11761 ggaattgagc attgcacgat gcaaaatgtt caggggcata tttgccaaat aaatggcaat
    11821 tgctctcgtt ttattgaatt atggaatctg gtttttattc aatacaaccg cgaagcagat
    11881 aacactttaa ctcccttaaa aaacaaattt gtagatacag gtgctggatt tgaacgctta
    11941 actcaggttt tgcaggataa aaatagtaac tatgaaactg atctctttat gcctataatc
    12001 aatagaattg aagagctttc tggtgttcag tatacaattg aacagggaat gcctcataga
    12061 gttattgcag atcatattcg ctgtctctgt tttgccttaa gcgatggagg tttcccttcc
    12121 aatgaaggaa ggggttatgt tttaagaaga attttaagaa gagctgccag atatggtcga
    12181 ttacttggtt ttgccgaacc ttttttgcat ttactggtgc ctattgtaat tgaacagatg
    12241 gcacatcact ttcgcgaact gagtgggaaa gaagattata tcaaaatggt gatcaaggca
    12301 gaagaagaac gctttaacag aactttggat accggcttgg aaaaattcaa tgaaataact
    12361 aagaaacttg ctggagatat tatatccggt tccgatgcct tcactttgta tgatacatat
    12421 ggttttccct tagatttaac cgctatttta gctgaagaga agggcttgaa gatagattat
    12481 caaggttttg ataaagaaat gcaagaacag aaagatcgtg cccgtaaagc cagtaaattt
    12541 actttacaga caagtaacga agattggatt gagttggcag aaaatcatcc tactgaattt
    12601 gttggatata ctgaaactaa aattaaaacc aatattcaat gttatgcagt tcaagaaaag
    12661 ggtttaattt taattcaact tgctgaaact cccttttatg cagaatctgg tggacaggtt
    12721 tctgatacag ggagaatata taatgatggc tttgaagttc taataaacaa tgtgcgcaag
    12781 caggatgaca aaatagttca ttatgggaag ctgataagag gaactataaa cagtgaacct
    12841 gttactgctg aagtatccat tgaaagacga aaatgtacag ctcgaaatca tactgctact
    12901 catctgttac atagagcttt aagagaagtt ttaggagagc atactgtcca aaaaggttct
    12961 ttagttcatc ccgattatct acgttttgat tttgcacatt ttaaggcact aacttttgaa
    13021 gaaattagaa aagtagaaga tatcgtaaat aatgctattt tggataaccg aaaagtaata
    13081 acaactattc agaatataga agaagcaaaa aatgaaggag caatagctct ttttggagaa
    13141 aaatacaaca acatagtaag agttgtatgt gtagaggatt tctccaaaga attatgtggt
    13201 ggaacacata tttcctttac gggtgaaatt ggtttattta aaattacctc cgaaagctct
    13261 tctgcagctg ggataagacg cattgaagcg ataacaggaa caacctcttt aaaatgggtt
    13321 caggatttgc aagataagct aaatcatctt gcaacaatgc taaatacacc tattaaaaaa
    13381 ttggaaaata aattggatgc aactttagaa caaattaact ctttagaaaa ggaaataaaa
    13441 gaactaaaaa acaaagaagg ggaaaaatta gttctggaat tgctatccaa agcaagaact
    13501 caagagaact attcactaat tatagaacag acttcttttg ccaatcctga tgagctgaaa
    13561 cagtttgctg aacagctaaa aagtaatatg aaaagcacta ttgccgttat ctttaatcta
    13621 taccaaaata aactgaatat tctctgtgta gttagttccg acttgattcc aaagtataat
    13681 gcaggaaaaa ttgtagctat actagctagt gaattaaatg gaaagggtgg gggaagagct
    13741 gatctggcaa tggctggagg aaaagataca gctaaactta gtctcgtaaa agaaaaggta
    13801 cctgaactaa ttagttccat ataaatgaat atacttattc tgcggctatc ttcaatgggt
    13861 gatattattc tcactcaacc gatatgtgct ttgttacaaa aaagctatcc gggttgcaat
    13921 atagattatc tttgcaaaga gtcatttatc gagcttccag aaatgtttga tcctcctgta
    13981 aatcctattg tttatcaaaa gacgctgaaa tttcatttat ggcttttcaa gagtaagtat
    14041 gatatagtta tcgatttgca taacaagttt gctacctatc tgattatgct ttttattaag
    14101 gcttcccaga aagtgcacta ttgcaaaaaa cgcaagttca gacagcggat agtgaaaggt
    14161 gataaaaagc taaaaattga tagcacggtt tctctttatg cctctgcttt aacgaaacta
    14221 ggaattaaac aaccctggtc ctatcctcgt ttacagatta gcagtttgaa gctatcaaat
    14281 tccatatatc ctacaaatca agattgcaaa gatatcaaac caattaaaat tgctgttttc
    14341 cctggtgcta atcactttac aaaaaggtat ccagttcttt cttggattca attaataaat
    14401 tctaatcctc aatattgttt tactcttttt ggttcaacaa tggataaaga tatctgtgat
    14461 aatatcagaa agcactgtcc tggaaactgt atcaataaat gttccataat gtctttgtct
    14521 gaattgtata gagaactatc tacctttcaa gtaattatca gtggtgatac cggcccaatg
    14581 catttagcag caactttgaa taaaccgcaa atatctatct ttggaggaac tcatcctcgt
    14641 ttgggtttca gacctttaaa tgataaagca atagttttgt gtgctgattt accttgccaa
    14701 ccttgttctc ttcatggaag agtaaaatgt cctgaaggtc acttcaattg tatgaattca
    14761 attaatcctg cacttcttag ttctgcaata aagaaagcgt tatcttcctg atccttagta
    14821 atctacaata taaaacctat gctcattaat aagtgatttc gtagcgaaat tgaaagcaaa
    14881 aaagcagtgg ctttatgcgc aactactttc aaatataatt acaaaattgt attaagcagg
    14941 tgctcttaat tttttataaa gttgtgaaac gcttattaat tggacaccca tatcatccat
    15001 ttgagccaaa aatttctgca aggcaaccaa tttctcgcga ttgtgacaat gggtaataat
    15061 cacgatcggt tcatttctgc ctttatattt tgccaaacct tcaattttat tgattatcgt
    15121 tttatcacta ttatcgggaa catccaaaaa tatatctctc ttagcggtaa tcaatcctaa
    15181 attttctgca gcagtgtaag tagtactttt attggtagtt gcgctatcca gaaaaaatat
    15241 tcccaattca ttcaattcat ccagaactat actcatcgta gcaatgtctg atgttacttc
    15301 actgcccata tgattattgg ctgcaatggc attaggaatt tgagcataga aatcctgtag
    15361 taaatctgct atggcaaatt tatccattcc attctttatg aatctctttc cgggattagc
    15421 tttatgatca attgcttgca taggaatgtg tattaaaact tgatgacctg tcttatctgc
    15481 aagacgagca actgtttgag tatgaggtaa atctggtaaa atagcaaaag ctatttcttt
    15541 gggaagggaa gtgaaatctt ccagtaattt tcctgaatat tgaccaaaat catcaataat
    15601 tatagctata ggaggcatat tatctgattt agaccaagtg tatttatagg atttaatttt
    15661 agtaatatcc ggagaagagg tagttttctc ttgatcctga ttctctattt tttcagtatt
    15721 actttctgga tgttcagctt cggatttttc tatagttggt tctgtctttg gtttactatt
    15781 gcaactaaat aacactatgc taaacaccaa gataactatt aggagtgtct tttccaagtt
    15841 actttatctc cagtttgaat attatatttt ttaactgttc cggcattaac ttccaaaaca
    15901 aatttatgag taaaattggg ggtaattctt tcttcactga aggggactgt attttcgtag
    15961 atatagttta tagtaccatc tgaagctata aatagcatat ctaaaggtag atatgtatcc
    16021 tgcatccaaa attcatagat atctggatat tcaaaaataa acagcatacc ttgattttca
    16081 ccaagctgat ctctgaattt taatcctcgc atcagctctt cttccttttc tacaatttca
    16141 attgagaatg tagctttaac agttcccata ggattagata tgttcaaagt tccatcttgg
    16201 cgaaattcaa ttttactgga tggttctttt ttttgaggct ctgctctttt acaaccgaat
    16261 agtatacata aaacacagat caaccaaacc gagagtatta attttcgcat ttatatcgta
    16321 ctatttagat agcagcattt tacgagtttg ctgatagttt ggagtgttta gtcgataaag
    16381 ataaactcca gaactaactt ctttgcctct atcatctaaa ccgttccaaa cgacactgtg
    16441 ttttccagag gataaaacgc cagaaactaa ccgacgaata agctgtcctt taaggttata
    16501 gatgtttaaa gtaacaggtg tattgttttc agcaagatca aaatgcagag tagtttcggg
    16561 attaaaagga ttggggttat tctggaaaag gtaagtaaac ggttttggta aatcagaacc
    16621 atcaatagca tcactcaaat taacatattg agaacctgct actccattaa ctgtatagta
    16681 taaagtaaag ttctgcggat tggttcctaa aattatatat aaagaatcct gatcaattgc
    16741 gtaaccggca aacccgtaag aaccatcatt attattaact gcttgcacga aaggatggtt
    16801 aaaagtgatt ccctgatgca aaacctgatt aatagtattc ggagcggtag aaactactag
    16861 ggatttcagc tgatcgatat catcaaaatg caagtttagt ttgttgttct cataagtagc
    16921 attcataatt ggaatagtca cttgttctgg aattccagat gtagcaggta aaatataatc
    16981 cggttcatag atgtatttac aaagcaaata actatcgaaa gaatcaatac taccattatt
    17041 gtctacatca gcattagtaa ttctgaaagt atcccagggt cttggatcaa gctctataat
    17101 aggatcaaga ccaattgcat attcattgat tgcagctata tcctgtatat ttattctacc
    17161 gtcgatagtt acatcaccat atataggtat attggtattt atccctgtca ttgctatatc
    17221 atctatataa atgccatcag ctgtttgaga ccaatcactt tggattctga atcgtaagta
    17281 tacttgaccg ttagtatagt tcgaaagaga atagttctta tttacccagc tgcttattat
    17341 tcccgtaaaa ctatccaaag cagtccaatt tgtactatta gccgaaacct ctaaatgcac
    17401 ataatcataa ctgctttcca attcatattt acagcggaat gaaagaacag gattaatgat
    17461 tccgctgagg gaaacaggat tagttaatgt aacactgcga ttttgcatat tactgtaatt
    17521 accagaagga gaatcggtca acacattgga tccttcgtag ttcataattc cccagtttcc
    17581 agtggccgtc cagtttccaa gtccattctc aaaatcttcg caaaaaacag gattttgcat
    17641 aataatgata ttacggtaat cattctgtct tatttgaata tcctgaacaa tttttgtata
    17701 acccgcagca gaaatactta gctgataatc accttcataa atggaaaaag aaaataagcc
    17761 ctgagtatca gtctgaacag taatttgagg atcactatta agtattatat ttgctccgct
    17821 tacaggatta ccttgcaggt tgcgaagttg accttgaaag ttagtaatca tagcaggttg
    17881 caaagtgaag ttttgtgtaa ctgatccact ttgaggtact gttacagtaa cagtttcagg
    17941 aataaaacta ttagctgagg cagttatttg ataagtgcca ggtagcaaca agcgatgata
    18001 atctccaacg ggaaggtctg tatgctcaag tttggaattt cctccaaccg taattgtggc
    18061 tgctattggg gttcctaacg aattagttac aattccggaa acccctcttt gagcaaattc
    18121 taaatatttc agtattgctt cttcattatc tgcccaatat gaatcaagcg ttgaagcagc
    18181 aggccatttg ttagttccaa tttcggcagt taattcgatg caatctgtat aatgataatt
    18241 ccaatcctgc atacttcctg taattacata ccaagcagct ccatttgtta ttccgtgagt
    18301 gaattcagtg ctattgtaca taggcaaatt ctcacgagca taagttagtg ccatttcagt
    18361 aagtaaatca ttatctggcg ttaaagcata agtataatcc caaggataat taactactaa
    18421 agcaccaccg tgaaagttta ttgataaatc aaaatcgtga gcattgctaa aatccattac
    18481 tgcgattgtt tctactgccc aagattctga atctggatgt tgaactcctg taggcatagg
    18541 gaaattcctg ttcaaatcaa tcccgttggc attataacga cttactgcag cataaccatc
    18601 tggattgagc attgggttta tccatatttc agtgttattt acgatattgg ttatcctggg
    18661 gtcaatccca tattgagagg tgagcaattg aataagacga ataagcatat cgtaaccaac
    18721 aacttcatct ccatgaatac tgcttatata tttcagctcc ggttcgtttt catcgaccat
    18781 aacattatca cttattttca tcatcaacaa aggtctattt tgaacgctgg atccaaattg
    18841 taccagttga cagatattag gatattgagc agctatttgt tgcataaaat tttggtattc
    18901 tgttaaagta taataagcat agcggccatc cagatcccct tttttcagtc ctttaaaata
    18961 ctcatcagca ggattgggta gtaattccgt aattatccct gcagctgtaa ttttatctct
    19021 ttctgaatca ttacgaacct cgatatggat tactcctgtt tgcagattta cataatcgat
    19081 gctaactttc aactcattaa gctttttgat atctgcttgc aaattccagc tttgaattcg
    19141 caaaggataa gcaaaaatgg aagcagctat gattatcagt ccaaaagtta aagctatttt
    19201 tttcattatt tttaatcctt gtataatgaa gaattatttt catatattac agcataaatt
    19261 atgccatatt ggttggttat atagataaaa agccaggcaa acaatgtcac ataactggaa
    19321 aagcttaatg ctgcttcatt cctgatctga catagttcac ttgcagttat tgcattgcgc
    19381 ctggctaatt ccatgaaatt taggatattc attctgtcaa ttaaaaatac aatctcaaag
    19441 tagtggataa acgccaatct gcagcttcgg aacattgttt aaccatctta aagaacttat
    19501 ttgcactaaa gttatgaaaa cttaccgaat ctatattctc aaaattatca atgaagggag
    19561 caagtttaaa ttcgattagg gaattattat taaaaaggca agcagaattt aaattaaaag
    19621 ttaattcctt ccaggtaccc caagtatatt gaggagagag agtccagtat aaatttttta
    19681 tgtcgtgcct gaaatcaata cttgcagtcc accttctgtt tttagtttgc agttcataat
    19741 aggattgttt ttgttgagcg tattgatttt cttcgtatgc aaagcccaga tcaatcataa
    19801 aagcattttc tggactattg cgaaaaggaa gttctagcca acgcggatct aaagttcgag
    19861 caattaaagg aaaagtatcg aacaccagat tatattgata gcccaattga cggtaaagag
    19921 tattattttt accagtattg tatatataat cactataatc tgctcttatt tgatatgtct
    19981 ggaaaaaagc tatcctgtct ccaatcagaa acttgcattc cggaaacata gtccagctgc
    20041 ttatctcttt attgtttgcc gatagcaaag aatctatata cacatcttta ctaaagctgt
    20101 aaccaaacca gtttcctaaa actactcgtt cgtgccaata atgtttccag ccaaatcgta
    20161 agttatgggt tagcaggtca ttatcccatt ggttatcatc ttcggggaat ttgattattt
    20221 gcaaattaac aaaataagca gcaattaatg aatccccttc actgtattcc caatttatcc
    20281 tgctaccaat atgtctatta tctgtttgcc ttgtattcaa atcatagtaa tagtccttaa
    20341 tagcataagt atgagcaaga tcgctttgcc acgaaacttt ttgcgaaatc tgataatcta
    20401 agtgtagctg tgcctgatta tagtgatcag tattattacg aatttcactt ttactgtagc
    20461 ttgtttgcct taaactgtaa ttatcccgta tttccaattg aacacggtct tcaggagaat
    20521 attgtagatt tccagagata tcaaaattcc agcgtaactg attatccgat agctgataat
    20581 tactacgaat ttcattttgt tccggagccg ctagaacata gatatcttcg ctatgataat
    20641 tataatttgc caggcaggca aaattcagat aatttccgta ataattatag ttcgctccca
    20701 aattagcata gtcaaaggat tcccaatcca ttcttttgcg ttctgcattt gctgatatac
    20761 ctgctatgga atttaaaata tagttagaat aacttgcatt ggaaccaaac caatatccct
    20821 cgctggaaag agaatagcca ttcacataac ggtcttgttc atttcttaat atacctttgc
    20881 tgaaaatgcc aatatttaag ctgtccacag gactgtaatt tatatgataa cccaaaatag
    20941 cagttttatt tacataagca gcaggttcca aatcgctatt atcataaaag gtttgataat
    21001 ccgaactaat agtatgcata aagtgtttac tcattaattc caaacctaca ttcaaatctg
    21061 catttttggc ttcctgatta aagtttaacc gattttctat gttgcttttg ctgttaatta
    21121 ctaatgaagt tttagctgta ggataaaatc tatactgtaa agcattttct atgctgagaa
    21181 cttgttggct ggaatatact gtccagtcca atccctgagt ttgagctttc caatatggac
    21241 tacaaagaag aaacaagctc agaaagaaga ataaaatcct tttcttcaag ctcttctcct
    21301 ctttggcgaa agttgatttg cgacaaattc tccagttctt tggttttttc ttttcccaaa
    21361 acagggataa aattgttggc tagtgttttt ctgcgatggg caaaagccgc gtctataatg
    21421 ttattaaaac tttctggata tttaattatg ggtttatcgg ttcgagggag taatagaata
    21481 actgttgaat ctacttttgg cacaggattg aacttctctt tatttacctg tattttgcta
    21541 taaatatcat aaaccaaatg caatcgaata gttaaaggag aataatgttt gctgtgggat
    21601 tgggcacaaa gtcgctgagc aacttctttt tgaaccataa gcacaattcg ggaaaagaat
    21661 ttagaatact tttctaactg tgctaaaagc ggactggtta tttgatatgg gatattggct
    21721 atcaacttta aggggtatcc atcttgttga attaaggaat cccaattcgc ctgcaggata
    21781 tccgtacttt ccagaacaaa tctcccctga taatgttcag aaaggtattt ctgcaagcgc
    21841 ttatcaagtt caaaagctct caattgaaca ttccaggata gaatttcatc ggttaatata
    21901 cctgtcccgg gtccaatttc ccaaacttta tcacctttat gtaaatctcc caaagctaca
    21961 atttcgcttg cgattgtttt atcgattaaa aaattctgcc cgagcgcttt aagagctttc
    22021 attaaagaga aaaaatcgct ttcgttctaa ttttacaaat tcaaaacgaa agcgaaaaca
    22081 agagattacg cgcttaattt tttagcagcc acacttttac aggttttgca aaccttatat
    22141 gtcttctcac caactttaac ttcattaagt agttttatac cggttcgatg acatacaggg
    22201 cattcacctc ttccagaatt aggaagtttt tttattcctt tgccacgatt atttttcgcc
    22261 attatattct ccttatgggt taatatacta atttttaaaa ccatatctga aaaaggtgac
    22321 ttttcagtca atgctttttt ccgggagtca gaaatctctt gtatctacga cattttccct
    22381 ttttattatt taataagctc aaagtgctaa ggatgatatt agctaattgg cctataacaa
    22441 tttatagccg gaataagaac atatatggta acaacttaca attgtaatga gccctcactg
    22501 ccaaacaaat tatcgggaaa atactagttt cgaatggcag cgaggactcc ttgcttttaa
    22561 tgagaacatc attcattcag ttcatcgttt aagctcaaaa gtatcatcta tactcaatac
    22621 gaattcaacc aataactgca cagcattatc caaatcagca agagagataa tctcattagg
    22681 agtatgcata taccgattcg gaatactaag tatggctgtt gcggttcctt gtctggaggc
    22741 aaaaacagca tcagcatctg ttccagttcg ggaaggggaa atttctatct gaagtggaat
    22801 attatgttct tttgctaatt ctgtcagttt tttgtttatc gcaggatgca aagcagaacc
    22861 caaacagagt acgggtcctt tacccaaaga aacatcaccg taaatgtgtt tatccactcc
    22921 aggaatatca gaagtgaagg ttacatctac agcaacagca atatcaggat caatccgata
    22981 agcacttgtt atagcacctc gcatagttgt ttcttctccg acagaggaaa cagcataata
    23041 atctgctttt atatcttttt cccaaagttc tttcataact gcagaagcta tataaacacc
    23101 tactttgtta tcggtggctt tacttacaat cagttcttca ttcagttcct cgatttgtga
    23161 gctgaaagtt atagtatctc caatagaaac aaggttttgg gcatcttctt tgtttctagc
    23221 accgatatct atccagaggt cttctaatct caattttgaa ggttcatcac tattgcgtaa
    23281 taaatgaatt ggcttttttc caataatacc atgtactatg ttatcgttat gatgaatatc
    23341 tactcttaaa ccaggaagca tatttacatc gaaaccgcct aatgctttaa cataaataaa
    23401 gccgctttca tcaatgtaat ttaccataaa tccgatttca tcagcgtgac ctataatcat
    23461 tattttatgt ttaccactgc cttttttatg agcaatcaga ttaccattga tatcgatagt
    23521 tatatcttca cagtaatttg aaagataagc attggttaga acttgagcag gtgtttcaaa
    23581 accagaagga ctgtgggttt gcaacagatc aaacaggtat tttttatttt cgctgttcat
    23641 ttatgtacct tagcagttat tagcttcctc atccgcaaag ggattgaggg ctttttttat
    23701 taccggtttt tcgtgcggga ttaaacagag aaactttagg gtttcttttc ctacattttt
    23761 aaaagtatgt gttaaattgg gctctacgaa aatagcgtca tctgtgctga atgccatttc
    23821 tcccctttct gtaactattg caccatttcc ggaaaggcaa taaacctcat gttcccattc
    23881 gtgttgatga aagggggtat gtcctccggg ttcaatttca aacatacgca ttgcaaaagt
    23941 aggagcacca tctttttgag caataagcca acggatttgt gcgcctttag ctccctctgc
    24001 atctacgggt tccagttccg cctcatcgta atgaataact ttcatttttt cctccttaag
    24061 gatttgtgtg ttttatagat aaactaaagc gaaaagccaa aaaaccaaat acttaagtaa
    24121 ttacttttgg gtaagcaatt accataagat cttgctctat tttacgatag ctgatatcgc
    24181 ttagtaaaat tgcctgatta atattactta gccccaaatt cgggaatgga ctttttgtcc
    24241 ctcccaaaat tttggctcca tagataaaga tgaatttatc gatcaaatta tcttctaaaa
    24301 aagcttcagc caaaccacta cccgtttcca ataaaatgct gtaaaaattc atattatgca
    24361 gcatatttaa cacttctgat aaattaaaat gaccattctc acaatccaaa ccatgaatta
    24421 ctgcccctgt ttgtcttagt acttttgcct tgtcagtttt tagcattgaa tttgagcaga
    24481 taattaaacc gggaaattcg gatagagagt taataaatct ggaggaaaca ggaattttta
    24541 ggtaaggatc taaaacaact cttagtggtt gttttgggtt cccaggtagg cgagcattca
    24601 gcagaggatc atcagcaaga atagttttta ttcctgttaa tataacatca ttttcactac
    24661 gcaatttatg aacatatttt ctggcttttt caccagttat ccattttgaa gagccatccc
    24721 cggcagcata ttttccatcc aacgaaagag ccgtttttaa agttacaaag ggtctccctt
    24781 tttcaatatg acataggtaa tattccagct gcttttttat tttatcttcg tgaaatccgc
    24841 agtgaacctt gatccctgca ttttttagca ttgctattcc atttccagaa actatgggat
    24901 tgggatcagg aattcctata taaacttctt gaatgcctgc ctcaattatt gcttttgcac
    24961 aaggaggagt tttcccataa tgagagcaag gttctaaagt tacaaaaata gttgctcctt
    25021 ctgcttcttt gccagcatgt ttaattgctt gaatttcaga atgatcaccg ccatattgta
    25081 aagtccaacc aattgctatc accttgttat tttttacaat tacagaacct acaaagggat
    25141 tgggagagca tttaccccgt gctttttcag ccgcttgaat tgctaaatta aagtacttat
    25201 ttatttcaaa atcagtcact taagtgttcc agattcttac gggcaagttc aaaggcaggg
    25261 ttctttttaa gaatttcttg ccataattgc ttggcatatt ttaattctcc attacctgca
    25321 taagcagcag ccaagttatt tagcacattg ggatcgtctg gaagcatttt ttgtagctct
    25381 agcagccata cttttgcctg ctcataatcc ccttttgcta aataatgtga acttaagtgc
    25441 aagtaaggtt cctgccaatc attatccaaa gcaatggaat taaaaaaatc cacagttgcc
    25501 tgatcaagtt gttttaccat ctgccaatat aatgctctgt aataatatag atctgcgcaa
    25561 tccggatata tctttaaatt ggattctatc aaatcccatg cagttgcatc atctgctttt
    25621 ccaattgctt cagctatatt ggagtataat ttattttgtt taaaatacgc ttcgggatta
    25681 ccggctatgt tttgtcgtat tttccaatca cctttaatgt tgctaagcac caaagtccaa
    25741 tcctgtttat gattaagttc catataaaca atagcgctaa ttccatcttc gcctaatcca
    25801 ctatataaca tactgtatgt agatatttta ctaaactgcg gaatagactt tataatttct
    25861 gtgtatcttt ctgctaaatc atatagggaa ctttcattta ttgtatattg gcgtaagtta
    25921 tcccatttaa gttggattag agaattcatc catttcccgg ctattgtctc tggatcttct
    25981 tttgaggaag tgggggagaa gggcaaattc aatttttgca acagataatc cattggaaaa
    26041 tattgaggat actgaaaacc gcttagccaa cttaacattt cctgcggcga ttcaacagcc
    26101 atcttttctg gggttatatt ttgcagcata ggattttctc taccaatcca aagatcaata
    26161 tgtagtttta tcacattttc tgcttcagct cttgaatcag ctttgaatgc aggaaaagga
    26221 ttactggcat ctcttttagg agaataggga caactatctg gacagtgggt atcacatctt
    26281 gtttcattac aacattgcca gcaaatattt tttttcttac gcggacattg tcgcatagct
    26341 cgttcattgt aacaggattg acagcggttg ctcttaaaca atttcaacat ttatatccgc
    26401 tccaaacaag gtggcatttg ccttttaaaa ttggatttct ctatcatctg tttcactttt
    26461 gctatcgtct tttcttctgc atccggcttt ggcaaaccct cagtaagata ttttaagatg
    26521 ctatccaaaa caggatatgt tattcccatt tctgtttcat cactttgccc tgcccataaa
    26581 tctgctgtgg gtgttttttg aatgatctta tctggcagac caatttcccg ggcaatttcc
    26641 cacacctctg ttttgtataa atgagcgata ggctcaaacg cacaagcaga atcgccaaat
    26701 tgcgtaaaat aacccaccaa atattcactg cgattacttg tgccaatcac taaggcatta
    26761 tgttttgctg cttgatcata aagaatgttc attcggattc ttgccatcca attacctttt
    26821 cgtaaagagc tggcatcggg agcataatgc tcaaaatatt tttctaccaa atcatctatc
    26881 tttatcgttt gcagggggat atcaaggtga tttgccatta ataaagcatc tgcagcactt
    26941 tccggatggc tatttcgatg aggtaaatta taggcaatta cacatttttt gcctattgcc
    27001 tgaactgcca acgcggcaac taaagcggaa tcaatcccac ccgataaacc aatcagcaga
    27061 ttggtaaatt tggtatttgc gcaataattt tgaataaacg caactattcg ttcaatttcc
    27121 tttctatagt tcatcttagg ttttactcct actttgccat ttatcgatgc taattatcct
    27181 ctttttttca gcccgcattc gtcaagcaat ttcttgtttc tgcctgtatt ttcgtctttc
    27241 aaaccactct tctaccttgt tcaatataaa cagtacagta acacttccac catatttctg
    27301 taacgcgatt agagaagtgt tacgaaagtg tgagagagtt gttaggtaat caactaagtt
    27361 gcaatcactt atctattatg ggtggtaaca gacataattg atggctattt atctgcatat
    27421 tatagatcta actgtctatg tcgttatatt caagttgaga aggttgagag ggtagcaata
    27481 gcttataaat tgagagaaag gaaaatgtct aaacgaaaaa agataggaga aagtgtaaaa
    27541 aatattaggg gagattaacc gataaatata actgcaataa agagttgtcc aattctaaat
    27601 ataaaaaaca tctgtgggtt tctgacgaaa aaagatagtt acatataaag aagctgaata
    27661 atccatcctg aagtcatttt atcaaatttc cagatacgca aaataacaat tacagtgtaa
    27721 caattttttt cccctttaag tatgatagat aataacgacg gattttgaac ccagcgaaaa
    27781 aacagtccat tctttaaaaa acataaggtt cccatataca atatcaatta ccttgtctgc
    27841 caaatttatt acttcatagt tttatctttc attcattacg gtttattttt gggtaggtta
    27901 tcattctgat gtttattgtg acattcttgt tttaactctc aattttgaaa tagataaacc
    27961 agttaatacg agtatgcctt actgtaaatt ttaggaaaag ttacattaga tcaatacata
    28021 tcaaaattga aagtcaaaac aagcagtttt cagatgcaac aatctatcag attatatctt
    28081 tgccttagag agtaaataat ttataaaagc agaacaagcc accaacatat atttagcatc
    28141 agtaaaatct ggaatactat ctgttccatc aaatatacaa tgtcttatat cggatttctt
    28201 atctgaagca tatctctctt atcttttgaa atgtattagt aaaaattgat atacatatcc
    28261 aattggtttt cccaatttat ttttagccaa atttggaaat actaccacct ccaatttata
    28321 gtggattatt catcaagata aaaaagcata ataggatcaa taacccaaag caattgagat
    28381 ttgctaacac aatgaatatt aacaacatat aaagatagtc tgaattataa accttgcttt
    28441 ctcttgcagg ttactcagat acaacttgtc atccactcca cctcaactca tccttaacta
    28501 cgggataagg aggagaggaa gaggagagga ggatgggttg tttgtttaag ccatcttaaa
    28561 aatggtggac taaaattaag gagaaagaaa tgaacaaaaa ttctactgaa aggatgcaag
    28621 ttcgctacag cgatgccttc gaacctaaag ttgtttatat atctgggtaa tctgcgtgaa
    28681 ctgttttcgg tggcttcggt ggctagaaaa aaaatgcgga ggaacggcgt cctccgtcta
    28741 cacatctaaa tcctaaaatc ctaaaatcct taaatccnnn nnnnnnnnnn nnnnnnnnnn
    28801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28861 nnnnnnnnnn nnnnnnnctt aaagtaattg ggttcggttt tctcaccttc agtaactata
    28921 attatcaaat caggagcggg cttcctaatg ttatatggtc ttttagttct gctcattttt
    28981 agttttatct ctgctttttt tgaataagtc aacaaactct gaatattcta cataaggcac
    29041 agctccatat tttccacgaa tatagtcctt ggcataagaa gcatctttgc gggtagaacc
    29101 tttcggtagt ttatattcta ctaaagaata aagattggat gaatgatttg gtgctttttc
    29161 cacaaaccaa atttcatccc gtctaaaatt atctttattc agaaagagag tattttgggt
    29221 cgtaaagatt aattgagcac ccttaggatt tgtctcttta tcctgaaaca acttgatgat
    29281 gattttggtt aagaaaggat gtaaactgtt ttcaatttca tcaatgaaca accttccgcc
    29341 tctttgtaaa gtagaataca ataaaccggc aagacaatag attttttttg ttccttccga
    29401 ttcttgcttt tccatatcaa attcaacttc tttcagaaca tcttcttggg tatcatagaa
    29461 ttgatggatg gacttaatga atagatgaaa cattttatta ccccctactt tgataaataa
    29521 tgaagaaagt gggatattta cttttgtatc catttccatt tctttctcat tgatcctaat
    29581 ttccttaata tctgaatcag ctttacgcat aaattcattt acccaatcta agggggacaa
    29641 cattattgtc taataaagca gttgtattta tcggattata agaatcaata taccaaaggt
    29701 ttgaatcaaa aaaacatttc actatctttt gggcaataaa accattgaac tgagacaata
    29761 agctgatgaa aagagagttt tctcgtaatt tatcttttat ctctccttta ccttctttga
    29821 atttattatg gacttggatg tcctgcccca atcgttcaaa aaggactatt tctcttgctc
    29881 gggaagttgt tttacgcatt agtaaccact ctttagcaat atgatctcta aaagccgaaa
    29941 atccatagcg ataaagaaca tcatccacca aaaaaataac ttcccaagag ctgggttcat
    30001 ctttaccagt agaattcagt aaaaagggct ctactaaaat cggctctcca atttgggatt
    30061 ctttgaacga attaagaacc atattcttca taaaggagaa tgctttaaat atgttacttt
    30121 tcccgctggc attagctcca tataaaacac ttgttttaag caaacgctgc tgagaaactt
    30181 ccatagtatg gtcatcaagc tcttttttgg taatagcttc catactcaaa gtaaccctct
    30241 ggtaaataga ccggtaattt cccacactga attctactaa catagcaatc tactttatag
    30301 tttataacta cataataatg aatttctgat atttgtcagg ttattttatg aaaaaactac
    30361 ataaatgaca gtttatgtgt tttatgcttg attctggttc aacaaataat atcaatatca
    30421 tacatcttgg tgcattgcag ataacttaga attctccgtt tctcaaggat attttttccc
    30481 tcagatattt tttacacatt ccgaacaaag atgataacgg caaaaacggt aataaatatc
    30541 tatcaaaccc tgctggtaaa gtgtcttatt atttacggct ttcacttggc tggaatttat
    30601 atgattacac ataaaacgag tgatataatt ttccggtaga gctttgaagg taagatagga
    30661 ttctaaaaca gattcaagca tttcagcatt ggccattttt tgataataaa gatagataat
    30721 cggtaagtaa atatttatat agatgttgtt tacaacagtg gatcccaagc tttttgaatt
    30781 ttgcagtaga cccctctgtt tttgttgaaa ctgctggcag aaattctgat accttttttg
    30841 aggattggga gttagggctt caacattgct cacaaaaaaa tttaacaatc cagcaggcaa
    30901 acagttatat agaaattcac caagtaaaat aagtcgaaat ataggatgat ttccaggtcg
    30961 gatacgaaaa agctgccaat ccacatttag attacgcgca taaaatcgct ggctttcata
    31021 attgcacata agtttttcat aaagctctgg gcttatacga ttacgactct tttgtagtaa
    31081 actggaagaa ccaacaaaaa tgctgatcat ctcttcgctc attaaacctt ctttatgcca
    31141 ttcgcgtaat ttgataaaag ggatactttg tgctaactga aaaagattaa atttattctt
    31201 atcgtaacca gcagcttcca tcattccttc atagaggact tgatcaaaat cgcttatagc
    31261 aagtgaagca ttaaatctgc gaactttgct cataaaccgc tgttttccat atagagtaag
    31321 aatactgaat aatctatcgt tatctatggc agaaagtaaa tcacagtaat catttccgct
    31381 ggataatttt ctatctccta tatcagtaat caatttttga atttcttctg aaagctgatt
    31441 tttcagttcc aggatttcta taaattctcc attctctttc attgtgtaag gttgaatact
    31501 gttatgattt aaaactatat gcaaaataac ctgattgtaa tatacatctt cgtgatgatt
    31561 atgtttcaac caatcttgag tgttttgatg aatctcgacc gctccttgta aatcctctcc
    31621 atccaatgaa atgatagcat ttacaaaatc aggtccgcga aaagtattaa attgaccctg
    31681 atagtgtatt ttaaggtctt taccgcttac agttttcaaa tctgtagcca gatggctttc
    31741 atcccatata tgataaagaa atttctcttc catcaaaaat atctctgtaa caggttatac
    31801 ctttgtcttg ctgatgcaga tttataatat gtttgaggtg aatagcgaat aggatttgtt
    31861 aaaatgctta tcagtttcat agtttgattg cgtgataatt tactacaact cttcccatag
    31921 taaaaacgcg aagcagtttc tatcccgtaa attcctttac cccattcaac atagttaaaa
    31981 tacagttcta gcatcctttt tttactcata cagatttcca taatcaccgt tgcctgaatt
    32041 tccagatatt ttcgtaaata actacgcctg gtagttaaaa aaatagttct ggcaagttga
    32101 ttgctaatcg tgctagctcc aaaacgaatt cttcctgctt tcttgttgcg ttgatatgct
    32161 tctttaacca ttttccattc aaaaccgtaa tgtttgtaaa aattaccgtc ttcaatagct
    32221 atcagcatat tttgagtgcg tttaggaatt tgctcgagag gaacatagta tcttttatga
    32281 atcggataac cacgaactaa atatcgttcc accatcaaag gagtaagagg tggattcaca
    32341 aaattgtata aaagtgaaaa aaaggcaatt actccccaat agcaaaggtg aagaattaca
    32401 ataaatcgta aaatacgcag tagcaaggga cgcctgcgtc tcaaacattc acctcaaaaa
    32461 tatcaggaaa aagtaggttg aaaatattta ataaacagag aaaacccagg agttttattc
    32521 tgctttggat ttatttacat aatcagctaa acgagctttt ctccgggaag cagttctatg
    32581 atgaataact cccttttttg cagctttatc taattgagaa tatagctcag acagtatttt
    32641 ttctttttct tcttcggtgc tatcactgtg aattttctta gaaagagttc taattgtgct
    32701 ctttacataa ttattacggg cagctctttt cttgtccgtc ttcattcttt tcataggtga
    32761 tttgtgttgt ggcatattat cctctctttt ctgaattaag tatttaattg agacaaatta
    32821 tattggaact tgtttctgtc aattaaaaag tgagattttt actcaatgcg caccttgtct
    32881 attaagaact atacagttag atttaattat ttcaaatgca aatgagaagt aaattccatc
    32941 tgtctaactt aaagaatctt attttatccg tagcgtaatt ttttcttgac ctatttgcct
    33001 attttgccaa ataggaaaaa aaggaagatg agtatatgaa agatgatcct aaatatcaat
    33061 atatgagcca aattatgaaa gcaatggcac atccgacgcg tttatttatt ctggatttac
    33121 ttgctgaaaa agaatattgc gtatgtgaac tgcaaaaaca gatcggaacg gatatttcaa
    33181 ccgtttccaa gcatttagct gttctaaaaa atgcaggatt aataagcagt cacaaagtga
    33241 ataatcaggt ctggtataat ttgctctgtc cctgtgtgct ggacttttat aattgtgtgt
    33301 ccgaaattaa aaattagatc caaataagag gtgaaaaatg gcaaacaaca atacttgtag
    33361 ttattcctgt aattgcaata caggagaggg aaatgacaaa tgcgtaacta atttaacaaa
    33421 aaataaggta tacgcttgcg ggggagcaag taatgtaggt atgatatctt ttgagcttgc
    33481 gaagacacta catctggaaa atcattataa aatgggttgt gcttcaggag ttgcagctgg
    33541 tgaatttgaa gtgcaaaata caattcaaca ggaaggtaat caggatcttg tgattgacgg
    33601 ttgtccgatt tcgtgtgtgc aaaaaatctt tgagcaaaag ggaatagaag gttttaagca
    33661 tataatcgtg acggaatttg gcattaataa agaagctgca ttcaattttg aacctgctac
    33721 aattaaacaa ttgatagata aaatattgca ttaagaaccg aagagcaaga taaaggactg
    33781 ttgcaatcat aatcatcagg ttaaggtatc agactgaatt tgatgttcct tttcatattg
    33841 actatataga taaggtgtac ttttatcaac catagagtac tatacccatc ttattatttt
    33901 actatcttcc caatattcta tttgccttta tgctttcggt aattataata taaacaagct
    33961 aaatcaactg taaagaaggg ataagaataa tggatttaag accgaaaatt ggtattagta
    34021 taaattatat gcatctgggc gattatatgc aatatcatat ccgggataca tatttggacg
    34081 cactttttaa gaatggcgcg ctaccaattt tgattcctcc cattgaagat aaacaggtct
    34141 taatgcaata tctggatatg gtatcgttca taataatcat cggtggttta gattatcccc
    34201 cttttttata caaagaaaaa cagcatccta aaacagaacc gatggaaatg catagagcga
    34261 tatcagattt atatctgttg gatttagcat tgaaaatggg aaaaccagtt ttgggtattt
    34321 gtgcgggcat gcaattaatt aatatttttt ttgggggcaa gctaattcag catttggaca
    34381 atgtggatat ccactatgga gaaaaaatgc acgatattta tgtgcaaaaa gacagctatt
    34441 ggcttagtca aataattccg gagggtaaaa ttcttgtgaa ttccaatcac catcaaggtg
    34501 tggatccaac tcatataggt aaagggctta aagttgcagc ggtgagttcg gatggctgta
    34561 ttgaagcatt ggaatataat tcggaacaaa tgattttagg aattcaatgg catcctgaac
    34621 gaatgcagga tacggatcac agataccgtc tttttgattt tatcatccat ttcaaccaat
    34681 aggaatataa atatttttcc attgactaaa tcgtagcttc aaaaataatg taaagcaata
    34741 gaaaaaaata tagttataaa gtgaggtatc aaatggctta cggaaagatt aagaatgact
    34801 tgcagaagct ttttgtagaa ttgaaagaac aagggcttta caaaaatgaa cgaattctaa
    34861 ctactcctca agatgccaaa attggcgttc aaggaggaac aaatgctctt aatttttgcg
    34921 ctaataacta tcttggttta ggtaataatc cagaagttat taaggcagct caaaatatta
    34981 tgaatgattg gggttacgga tgtaattctg ttcgttttat ttgcgggaca caagcaattc
    35041 ataaacagtt ggaagaagca atgtccaaat ttttggggat ggaagatact attctctatg
    35101 cagcttgttt tgatgctaac ggaggtctat ttgaacctct attaggtgaa gatagcgcta
    35161 tcatttctga tgaattgaat catgcttcta ttatagatgg cgtccgtcta tgtaaagcaa
    35221 aaagattgcg ttataaacac agcaatatgg aagaattgga atcagctcta aaagagagcc
    35281 aggattgcac ataccgcatc atttgtactg atggtgtttt cagtatggat ggcgatatgg
    35341 caaagctgga tcaaatctgt gatcttgcag ataaatacga tgctttggtt atggttgatg
    35401 attcacatgc aacaggttac attggaaaaa ctggaagagg tacccctgaa tattttgggg
    35461 ttactgatcg agttgatatt attactacaa ccttcggaaa agctttaggc ggagctaatg
    35521 gaggatgtac ttcagggcgt aaagaaatta ttgaattatt gcgtcaaaga agccgtccat
    35581 atctgttttc caacactttg gcacctgcta ttgttggagc cagcttaaaa gttcttgata
    35641 tgcttaccca ttccagtgaa cttagagata gagtatggga aaatgcatct tatttcagaa
    35701 aggaaatgtt ggatgcaggt tttgatattg ttcctggaaa tacagcaatt gttcctatta
    35761 tgctatatga agcaccttta gcaatcaaga tggctgatat gcttttagaa gaaggtgttt
    35821 atgtaattgg ctttgtgtat cctgttgtcc caaaaggtaa agcgaggata agagttcaac
    35881 tttctgctgc acatacaaaa gaagacctgg atttcacaat tgcagcattt aagaaaattg
    35941 gtagtcaatt aggcctgata taattgaaga aaagggtgcc gggtattttc tcggcacctg
    36001 atatattttt tgttatgaag tatggggata tatatttgat tcccgttcca attgggaatt
    36061 tgggtgatat aaccttaaga gcaattgacc tgctaaaaaa agcagaacta attgcttgtg
    36121 aagataccag agtaacttct tttctgcttc aacaatataa aattccaata cctaaactaa
    36181 tcagctttca taaatttaat gaaaaacagc gagaagaatt tctgttgaaa tatctagaaa
    36241 gcggagagga tttaatggtt gtttcagatg ccggaagtcc tgcaattagc gatccagcgg
    36301 aaaaacttgt taaagctgct attgataaag gtataaaggt ctttgcttta ccgggcgcaa
    36361 gtgcactctt accagcgata actgtttctg gtttctcaac cagacaattt caatttgtgg
    36421 gttttttgtc acaacaacct aagagcaggc gagaaaaatt acaagaaata gctgaatatc
    36481 cttatgctac aattatttat gaagcgcctc agcgtttaca tagattatta agggaactgt
    36541 tgctgcactg tggaaacaga aaaatatgta tttgtagaga aataagcaaa ttatatgaag
    36601 aacatatcta taccactttg gaagaaataa atcagaatcc ctctcttaaa atcaaaggtg
    36661 aattttgcct ggttttggat gggaagagaa ttaatagtaa caccgatgat gaaagcgata
    36721 aaattactga agacaaactt attgattata taaacgaagc tctggatttg ggactaaaaa
    36781 caaaagagat aagtgaaaag gtcagtttaa tgagttctct gaataaaaga gaagcatata
    36841 atttggtagt tgaaacccaa aaggaaagag ataattgatc ccctttttgc tctttgattt
    36901 tgacggcaca attgcagatt caattcgtct gggaattaga atagcaaata atatcgctcc
    36961 taagtttggc tatgaaccct tttctgaaaa agatttgaaa cggttccaat ctttaacctg
    37021 gcgtaacatt atatcagaat tacacttgcc gctttataaa attccaaagt taatttccat
    37081 aggactaagt gaatatcgcc atctgatttc tgaagttgaa ccctgtgaag gaattctgga
    37141 aatgcttaat aacctttctg aaagaggaat accgatggct ttactcagtt ctaatacgga
    37201 tgaaaatgta aagctgtttc ttgataaata taatattgat accttcaaat gggtggaagg
    37261 aactgcagga acactacata aagcaaaaca cataaaatac cagctaagaa agcataagtt
    37321 aaagcctcat aaagtaatct atgtagggga tgaaattcgg gatattcaag ccgctcataa
    37381 atgtgcaatg aagataatct ctgtaacatg gggtttccac tcaccagaat ttttgttgca
    37441 ctataatcca gatttcttag taaataaacc caatgaaata gtggaattga tagatacatt
    37501 tattcaagat gacttagcta taccatttaa taaaattaat aagaaaaagg aagaaagagc
    37561 ataactgtca agagattaag tttccattta gttaattgaa ctactacagt ataaaattac
    37621 tttttgaatc atttttaata gataagaaaa caataaaggg caagattatg atagagtgat
    37681 ctgaacatat ccctatagtg caatataaat aatgtagatt atagcgatat ctatctaact
    37741 actataatga tatgtgcaat ttgcagctga taattctgct ttaaaagagt atttatcatc
    37801 cccacctatc aatacaatag tatatactat actctaaaac gcagaaatag cattcatcag
    37861 gataaactta taagttcgta tatcttaacc gctatatttt tacctttaac tttaacttca
    37921 tccaagtatt tagcattaac taaatctttt accttctcat aagtatattc gctgattatt
    37981 atgtgattga aggtatcata ttctttattt aaactctcca gtcgtgctcc caagtttatt
    38041 gtatctccaa tggcggtata atcaaagatc tgttcacttc ccaaatttcc tacaacagcc
    38101 atccccgtat taattcctat tcccatttcg aatggttcat atccttcgct aatccatttt
    38161 tcttgaagtt gtataaggcg ttcacgcatt ttcaaagccg atttacatgc atcaagagca
    38221 tggttgggta aaggaattgg tgttccatac agagccataa tttcatcacc tacgaattta
    38281 tccagaatcc cattgttatc gataataata ttaaccattt ctgtaaagta ttgatgaaga
    38341 atatttacgg tttcttcagg agagtgtttt tcggaatagg tagtaaaatt tcggatatca
    38401 gaaaatagaa ccgttatttc ttgtaatgaa ccaccatact tcaaattttt gggattagct
    38461 aataactgat taaccagttc tggggcaaga tattgctgaa aagcgtttct gataaagcgt
    38521 ttttctttta gtgtatccaa atagtgattt aatatgctgg ctacatatat aagtatgaga
    38581 agaacaaccg tttgcgctat tggacaaata atattggatg aactaaagag caggtaagta
    38641 acaataaact caataatcaa taatccaatt agggctatag cggaaaattg tggttttaag
    38701 agtttaaaaa gaaaccataa accaattagt aatatgattt caacaagtaa atatagccaa
    38761 agattgagtt ctttcaaata actatcagtt aaaaccatat caatgaagtt tgcttgaatc
    38821 tctactccgg gggtccattc tcctccgtaa ggagtaggaa atttatcgtg caattcatca
    38881 attgttgctc ccactaaaac gattttatct ttcaggatac cactttctat aatgtcgtac
    38941 aattcattta attctgctcc ttggtaacca ggcataaaaa aggttgaatc atctaaaaca
    39001 gaagcatagg atactgatgg atatgtccct gaaggtcctc gaaaattgat aagagctttg
    39061 ttcttgtcaa taataggaat gcttttatcg gttaaatgta aagtccgatc agcaactttt
    39121 atatgatcag cgtaggtgct ttgatatact ctcaaattag ccagggacgc aatacccagg
    39181 ctgtatagtt tactttgatc aaattcttca aatagggtat acttacggat aaatccatct
    39241 ggttcagcac ccatatttac aattccccaa ggtaaattat aatctaatat agcattaatg
    39301 ggaggtatca tctgagttgg ttctcccgga tgttgagaag gcaacacctt tccagcaaaa
    39361 attacatttt gatatttagc agctgttttt gctaagcgaa gatcactttc cgggaaggaa
    39421 ttttcggtga attctacatc gaatataatc tgtttaacgc cagctttgtt caaattttct
    39481 atcaacttgg aatgatagtc cctgggaaaa ggccaagttt catctaaagc actaaaacta
    39541 gcatcatcga tagctataat agcgatatta tcggtggttg gtaatttacc tcttattcta
    39601 aatagcatat cttgagcttt gtattccaga tttttaaaga acgggaaagc aaagattatt
    39661 tttacaatta tcaggataac aaccaggaat ataatagctt ttctaatttt catataatcc
    39721 tcttatgaat aattgagccc cgtctgtcat tattaacagc tttctaacaa acggggcaat
    39781 aaattaatta gcattactaa aaccgctgag taagcagaac gcgccaaata aaattattgt
    39841 aatcgtaagc attggaattg ttattgttgt aatcggttat agcaatgttt gtattgatgc
    39901 ttaatgatct aaggggatag gcttctacgc ctaaagaata gtaaagataa tctctatcat
    39961 tttgagcacc acttgaacta ataattcgaa aatcagtata gggattaatt ctatcattcc
    40021 acaaactgta ggatgcactc gcaaagaagt tgcttgtttt acttttctta ctcgtcaaat
    40081 atagtaggtt ttcattatta ttcaatgaga aggcaaattt caaagttaga ggtatcttct
    40141 gaaaacggtt taacattgtt aggttaaggc tattattgtc attctctgtt aaatcgatat
    40201 tttcttcttc tactatttgg tagctattat ctgatccaat tttatagcta atatcaagtt
    40261 gggtaggaac atagggaatt tgagttatat tatagccaat gctgcaaatt aagctttgtg
    40321 atttattttc gtacttttgg aaatctgcat caatatcctt gttcatttca ttgtcactta
    40381 aattgttata ataagacaat ttgagataag ggattttagg tacacggaga atggattgta
    40441 aataccagga agaagtattg ttggtttcac ttttatggcc catcaaatta tcttctatca
    40501 aatttatacc accgcttaaa ataagatatc tggagatatt taaattatcg gtaaaagaga
    40561 taactctgct atcccgcatt tgatatgtgg agccaaaaga attgaaagaa gagcctgttt
    40621 ctgcatattg gacattaaat agattattcc aaaagtaagt tctgaaataa gcaagccaag
    40681 ctatactggc ccggctaggt agaaaaggaa tcatattttt attaataata aaaagatcac
    40741 ttagatcctg tggatcaatg ggaatatctt cacctatata ttcttcaatt tcttctttag
    40801 aaagagctcc aggaattata tttgtattca acaaggaggt ggcaatttca gctccaagta
    40861 ctatattctg ttctggaata tttagctggg catcaattcc gataactgca ttttcttgcg
    40921 ctttggtagt ataatttata ctcttattac cttcgtcatc ggtagtttca tacatataat
    40981 atacactatc aagagagctt acctcatctt tatggtgaga aaaatttaat ccaaagatga
    41041 atgaattttc gtctcctact tgaagtctgg tagctaaggc ttcctgttta aaagaaccag
    41101 atttctggat tccatcttca aagagatcaa catctaactc actggtagtt ttacggacag
    41161 tttctccctg tgtaacagaa aatgaaacag attcggtatg taaacgagta tatattcccc
    41221 ggatattttg accatttata gttaattgac tgaaagttgg agcataatca cctgcgaata
    41281 aatcaacatg aggagaatta actccaattg tataacggtt tacaggttga ctgttggaat
    41341 gctccaagga ggaaagatag atgtctgctt gtgcattaaa tttgccataa tttgcgtaaa
    41401 aatctgccca gctggaagca ttgcttttgg tatagccagc agcattttta ttagaataat
    41461 cataataatt ggaagcaaaa ttcacgcttc cttgataggt taatttgtta gatatccttc
    41521 cggagggtat aacttctgtt gaccaaattt gtgagtgaac gatatttttt tctttcttag
    41581 ctcgaaccaa agctcttaca attccgctgg ctggtttttc ttctttatat acaatcgtag
    41641 gaggtataat ttgagaatta gcagttacat ttttacctcc aacccaaacc tcaattgttt
    41701 tgggatcaat ttcatccgct aaagcaaaaa aggaaacaac taatagataa ccatcttctg
    41761 cagcaacaat tggattatcc gttagaagga caaatccagg atctgcttct ccataagcaa
    41821 tatccggttc taatatgtag tttggaacag tttcattctg aggtggaaag tattccttat
    41881 tgccctgtct caattctact acgaagtaat attctatcac attggtagtt aaatatttag
    41941 ctggaaaagt agcagtgaag tatacagaac cggggctatc ctgtttcatt tcttcattaa
    42001 accaatgatc ttgtttatca attctgtaaa taacctttat tttatctata tcgcttaaac
    42061 cttgctgtat ttctactttt atatcggtct tttggttaaa tgtataggtt tggggacaaa
    42121 tgtggactaa agatatagca aatatttgaa cagtaagaac catcgataac ataagaagca
    42181 aataatgttt aaacatcttt attctcctac caagtaattt ctatatactt agtctgtcca
    42241 ttcgggtcaa ttacaggaat acgcattttt ttattttctt taccctgagt tgattccagc
    42301 tccgcttgtt caatttcctg aagttcttct tgagtgctat tatgaatttg gaaactacca
    42361 ttagcttgaa ctacagcagt atttccagct gtaatagatt gcgtatcctt attcaatatt
    42421 ttaagctcta cttcaccttc agtaatgata aatgtagatt ggccgaattc atcgaatttg
    42481 gtaaaaaagg cagttccttt aaccgaggca actgtagtag gagtagcaac agaaaaagaa
    42541 cctgtattgg gttttatatt cgtaaataca cttcctttat ctaattcaat ttttttatct
    42601 aagatctttt cgttttttgt agcagtaata tagacgatag aattagagaa tatttttact
    42661 gtagaggaac catcaatata tttatatgca gcaaaagatt caccgcctgt acggatctca
    42721 tctttgttat ataaaaaatc acctgtttta tgaggaacca cttttgcggc tcttcttaaa
    42781 ttgactttac cttttgaggc agagatcata gctattgaat tggcagcaaa tatgttaatt
    42841 gctaatatta gtgccaagga aattaaaaat aaccgtttca tttttacctc cctcattatt
    42901 tctttagctc aacggagata tcttgtccca gtaaagcatc aatcaaatta atagcttcct
    42961 gaccactgta agttttgccc tctataacaa caactcctac aggaagatac ccttgggtgt
    43021 ggatattaat taaggttgga ttttgcagca gatcaagatg cgcttgaaat tctgaaatac
    43081 tatgggctgc ttcctgttca tccacatatt ggaaaatgta ccaattactg gatagggaat
    43141 tattcttgtt tcctcttgct attggattaa actcattggc aagggcagta gtaactttcc
    43201 aggcataata attgctatcg gtaaaaggca aaaagtccga aaagccgctg cactcatttt
    43261 tcccactagg ggtttcataa aatagcgatc ctgttctttc cacattgtta attgttggag
    43321 gattattagg tgcatattca cgaattatta gcttataatc attaaatccg gttatctgag
    43381 aattccataa aaagctgatc ggtaatccac taactttatc aggattttgt cctattggtc
    43441 ttccaggtgc aagtaaagta atatttccgg cattgcggat aattatttta aattctgcag
    43501 agggggaatt atctgctcct tcccaaggag tttcagaaaa ttctcgaacc caaaccttaa
    43561 atttgagttc tccatcggga aaatatcctg ccataacagc agatttcaaa gttgagctgg
    43621 catctaatat atcttctact gaaatacttg tactgccact tttgaattcg aaattgagca
    43681 ttggttcatc agtaatcaaa tcccgattat taagtgtgca ataacccaaa gtaaaatcct
    43741 ccctggaaca gaaaatagta tttgctaaaa gaagattatt ccaataaaga gcaactttca
    43801 ggtctattct gcagggagtt gtgttcagat ttttaatcat taaagtagtt aaaattggtt
    43861 gcaaatcagg ttgtgttgga tcaaaactag cagtgctcaa gctgtttatt ctaattgaac
    43921 ttggaacgaa attaacttca aaattagctt gtgcgttagc aaaaaccggt agcaacgcaa
    43981 taatcactat aatatatatc aagcgtttca ttttaaacct ccatacttga tagtaatatc
    44041 tattatattc tttaataaat cctggaaaga cattccagct gccttagctg ccatcggagt
    44101 taacgataga gaggtcattc caggaagtgt gtttacttct aagaaataag gtttcaggtc
    44161 atcatagcgg aaatcaattc tggcataacc gcttaaccca aaagccttcc atagcctgat
    44221 tgcgtaaagt tgtagaagct tagcaactgt atcatcaatt tgggcaggtg cttcgtaatg
    44281 ggttttccct gatgtgtatt tattggtgta atcataccag ccctcaaggg gtttgatttc
    44341 gacaacagga tatgcttctc catcaataat cgttacggtt aattccctac cagggatata
    44401 ttcttccaat aaaacattag gagaatactt aaaagcgttt tgtactgcag attttaaatc
    44461 ttccaaatat tccactctac ttattcctac cgaagaacca gcatcattag gttttacaat
    44521 aattggaagt cctaattgat tggaaaatcc cgaataatct tcagggtctt gataatccac
    44581 cagaaggtct tctcgcaaca aaatgtatct gggaactgga attccttctg ctatagcaat
    44641 cagcttggaa atgtatttat ccatactgat acaactggct ttgaaatttg aaccggtata
    44701 ctttataccg gccatagata gcgttgcttg taattcgcca ttttctccac tgcctccatg
    44761 taaaccatta aagacaagca aaggatgttc attttggata gcagttataa aattagataa
    44821 aactggataa ttcgcagggt ctatttctga aacaatatat cccatagttc ttaaagctgt
    44881 agcaacttcc gaaccgctaa ctaaggatat ttctctttct ggagaagtac ctccttttaa
    44941 aacaacaatc ttttccatct tacctcataa ctatttaaat taatagagta atccatatta
    45001 tatcctataa tgtagtacga atttgaaaaa atacctttaa ttgtaaatcc atagccgctt
    45061 tgaatctagc aatatcagaa acattagctc cacaaaaatc cagttcttcc agattttcct
    45121 gttttagaaa ttcgatcaat tttactgtgt gtaagctgct tgcacctttt tttaaagctt
    45181 caatctcggt agcgcggaaa atagtatatg ccttagcgca gtcttgtaag ataatattgc
    45241 tgctgacgat tctaccatct ttttttaaat ttagttgtcg taaaattcca gcagtatgca
    45301 gaatgcggat aaaatctgct aggtcttggt agcttaaacc tggctgccat ctttttttta
    45361 cattcataag tttcattaga tgaataaatt cttctatatt catttcttct tcgaaggtat
    45421 aatcttgttt ttccgccagt gtaattttct tacgttcatc agaaagaggg attatattat
    45481 tctggaaatt atgaataaaa gtgtaggatg gaatagcttg catattattc caaagaaaac
    45541 ctctaacatc ataagtttca ggggataata tgtggcgaat tactttataa cgacaggaaa
    45601 gatatttggc aattgtctga gtaatttgta aagcatccaa taatcttctt gctggagcag
    45661 aattatcttc cagccaaaga tttaacccct gataataaga taaaagagga tgtttacata
    45721 ttttacgacc aaacactttc ttttcataaa gaggtaggca agaaactaca ctttcgcctt
    45781 tgtaacatac aagatgataa ggttttagat cagaaatatc agctactgtt tgcataaaat
    45841 ctggattatt ccaaacattc aaagaactct tgcacaagaa agaaggttgg gttaattcaa
    45901 tttcaatcat tagatgtaag aaccaatagt gcttaataat tcttttagat gaaaaggttt
    45961 gataatataa tcggatgctc catctattat ggattggtaa gcagaatcta cagtagggta
    46021 tccggtcata atcaaacaaa atatcttggg atctctttcc agcaatattt tcattaactc
    46081 caatccatcc atccctggca tcatataatc tataatagcc atttgatatt tacccggttc
    46141 aaatttctcc aaagcataat ctgcatcctc agctaagtcg acctcatatc catggttcga
    46201 taaatattct ccaattactt cgcgtagtaa tctatcatca tccaccaata gaattcgagg
    46261 tctcataaaa actcctatct atataatatt aacccataac atcaaatata atcaaatcct
    46321 taaaatagtc aatataaaaa tgaaaaaagg ttggatctat ccataaagat tgtactctta
    46381 acggacgaaa agagtatagg atgaataatg ttattcaatt gtgttaaggc atagaaagaa
    46441 aagagctaat aaagttaatt atccctggga aaaaacaaaa accgattaac tcttcccaaa
    46501 cttctatatt tatgctatat tcaaacctat tgtgaaaaat aactcaatcc tgcgagcttt
    46561 tcccattttg caattagcaa tcttaatcca taataacatc actatcttgc tattttgctt
    46621 ttttatagtt gacatattta ggcatttata ttttaaggaa ttggattata aatcgtgagg
    46681 tgagtatgaa aggaaaagtt aaatggttta acaagaataa aggttatggc tttattatta
    46741 ctgaagacaa caaggaatac tttgtccact ggaaatctat agttactaat tcaccaagag
    46801 aattgaaagt actagaacaa gatgaattgg ttacttttga tctaatggaa actgataaag
    46861 gtattcaagc aattaacatc attagggtta atccctaaga gttaattacc taactttttc
    46921 aagggtaatg gcaaaacagc ccttaccctt ttttttacta atgccttatc ttcccataaa
    46981 taaatcagac ctgaaacaac gaggttggaa ttctttagat atcatcctaa ttagtggaga
    47041 tgcttatatt gaccacccca gttttgctgc agcaattatt ggccgtactt tagaagctgc
    47101 aggttttaga gtagggataa ttgctcaacc agattgggaa aatgattcga gttttcaagt
    47161 tttaggaaag ccaaatctat tttttggtat cactgcaggt aatatggatt ctatggtaaa
    47221 tcattatact gcacaaagaa aattgcgaaa tgatgatgcc tacactccag atggagagat
    47281 cggaaaacgt cctgaccgag caactattat atataccaat attttaaagc ggctttttaa
    47341 aggtgtgcca attgtaattg gaggtattga agcatccttg cgtagaattg cacattacga
    47401 tttttggcaa gataaagttc ggaaaagtat tctggcagac agtaaagcag atattttaat
    47461 ttacggaatg ggggagaaac caataactga attggctatg ttattaaaga aaggcaaaac
    47521 aatagatgaa atacaaaatt taacttccag tgttgttttt tctgctacaa atccatctcc
    47581 tgaagatgtt gtattaccag atgccgaaat atgtactgat aaaaatactt tttatcaaat
    47641 gaccaagatc tttgaaaaga actacgaaac aaaggttatt tatcaaaaaa cagctggcag
    47701 atggttgaaa cataatcttc ctgcaaaacc cctaactaca gaagaaatgg atgctattta
    47761 tgctttaccc tttgaatata aagcgcatcc tatttatgat gggcataaaa ttcccgcata
    47821 tgaacaaata aaggattccc taacatctca tcgcggttgt tatggtggtt gtaatttttg
    47881 tgccattgct tgccatcagg gacgcagaat tcaatccaga tcagaaaatt cacttcttaa
    47941 ggaagcacaa aaacataaag gcacaatttc cgatgtgggg ggccctactg ccaatatgta
    48001 tgcttcttac tgcagtttga attttccgga tagctgtcca aggagttcct gtttggttcc
    48061 ccaaatttgt cccaatttgc gtataaatca ttctcttcag cttgatgttt tagagaaaat
    48121 aactaattta cctaatatta aatatgtttt tattgcttct ggaattaggt atgatatggc
    48181 agtaaacgac gataaataca tcgaaacaat tgccataaaa tatacaggcg gacggttaaa
    48241 actagctcct gaacattctg ttcccaaagt tttacgctta atgggaaaac caaatattga
    48301 ttccttcgaa aagttttgca agaaatttta ttccttcacc aaaaaagccg gattgaaaag
    48361 gaaaatcgtt ccgtatctta ttattggcca tccagggact actatggaag atacatatca
    48421 gcttcgtaac tggctgatta ggaataaatt agttgttgaa caagtacagg agtttactcc
    48481 tacaccgatg agtatttcca cttgtatgtt ttatacggga ttagattatt atacaggaga
    48541 acccatccat attcctaagc catctgaaat acgtaagcaa aagaatataa ttgtagctaa
    48601 atataggcat taagttaatg tataacatta cattatgata gttagttcat taatagataa
    48661 aatgataata gatatttatc caccaagcct aagccgtagc taaagttttt ttagctcatg
    48721 ggttgctgat tatcgacctt ttaaataacc ttgagaattg gtttcgtagc attaatatct
    48781 tataacggat aggaatttac taccccacca tatcccattt gtttatatta agttaacctt
    48841 gatttatctt tactacacag cgttatgtag acgataatta tccgatatct atcccttatg
    48901 cgataaggga atcataaggg aagtataagg gaagtataag ggaagnnnnn nnnnnnnnnn
    48961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    49021 nnnnnnnnnn nnnnnnnnnn nnnnnaaaag ggggaaaaag gggtgagttt tagggcttag
    49081 aaaacagtaa gaataatgga tttataactt attatataac aagaggttgt aaatcataaa
    49141 taatataaga aagtacttga caataaactc atatcatata ctgtggcaac aaattaagga
    49201 tatattactt agagcttaac gcttttctgc taaccaatcc ttattattat ttgtaaaact
    49261 ataggagaca atatattatg caaagttcaa cttatacagc cagcaatatt aaagtgctga
    49321 aaggtctgga agcagttcga aaacgccctg caatgtatat tggaggaacc ggtgaacgag
    49381 gattgcatca tcttgtatat gaggtaattg ataattccat agatgaagca atggcaggtt
    49441 tctgcgatga aattatttgc acaattacga acgaaggaca tattagagta gaagataatg
    49501 ggcgtggaat tcctgttgat attcaaaaag atttaggagt tccagccgtg caagtagttt
    49561 taacagtttt gcatgcgggt ggtaaattcg atgataagag ctacaaagtt tctggaggtt
    49621 tacatggtgt aggtgtatcg gtagtgaatg ccctttcaga atttttggaa gttaatattc
    49681 ataaagaagg caaagaatac tatatgcgtt ttgaacgggg taaacctgta acggaattac
    49741 aaatattagg agacagcaaa aagtccggaa caatagtaac cttccatcct gatccactta
    49801 tttttgaaac tacagaattc agttttgatt atcttaccac tcgcttaaga gagcttgctt
    49861 tcttgaattg tggagtgcgt attattttaa aagatgagcg aagcggtcgt cttcacgatt
    49921 ttaagtatga tggtggaata aaaagttttg tggaatattt gaatcagaac aaaaaacctc
    49981 ttacccccaa tacgatatac ctctccaatg tgaaagaggg tatggaattt gaagtagcgc
    50041 tacaatataa tgaaggttac caagaaaata tcttcagttt tgccaataat atcaacacta
    50101 ctgaaggtgg aactcattta agcggattta aaagtggtct tactcgcgca gttaatactt
    50161 atataaaaaa tgctgatttg cttaagaatg aaaaggttag tccatctgga gaagatattc
    50221 gagaaggcct tacggcagta atttcaatta agattgccaa tcctcagttt gagggacaaa
    50281 ctaaaaccaa gctccaaaat tctgaagtag aaggaattgt aaatagcgca gtctatgaaa
    50341 aacttttgga atattttgaa gaacatcctg ctgaagcaaa aaatattaca atgaaaagta
    50401 tattagctgc acgcagtaga gaagccgctc gtaaggcacg tgaattaacc aggcgtaaat
    50461 ctgttttaga aagtggttct ctgcctggaa aacttgcaga ttgtacttta agcgatccag
    50521 caaagacaga actatttttg gttgaaggtg attctgcagg tggaagtgca aaacaaggta
    50581 gagatcgttc ttttcaagct attcttcctt tatggggaaa aatgctaaat acggagaaag
    50641 caagagtaga taaagttttg aataatgaca aaattcagcc gattatttta gctttgggag
    50701 cgggaattgg acaggatttt gatgtgagta aacttcgtta tcataaagtc attataatgg
    50761 ccgatgctga tgtagatggc tctcatattg ccactttact tatgaccttc ttctatcgct
    50821 atatgcggcc tgtaattgaa gaaggtaaac tttatattgc aatgccccct ctgtttttgg
    50881 ttcgtaaagg gaaacagaaa gtgtatgttt ataacgaaga agaacgagat gctgccataa
    50941 aggaattcgg agagaaaggt gtgattgttc aacgctataa aggattaggt gaaatgaatg
    51001 cagaacagct ttgggaaact actctcgatc cggaaaaccg caagatgatt gctatcaaaa
    51061 tggatgatgc tattgaagct gatagaatgt ttactatttt aatgggcgat gaagttgaac
    51121 ctcgtaggga atttattcaa acaaacgccc gttatgtaaa aaatctcgat atttaaggag
    51181 ttaaaataga tggcagatac aacaaatatt attccagttc aaatcgaaga acaactgaaa
    51241 caggcatatt tagattattc tatgagcgtt attgttgcac gcgctttacc tgatattcgc
    51301 gatggtttaa aaccctctca acgcagaatt atctatgcga tgcatgaact taatttaaca
    51361 cccggaggcc actttcgtaa atgtgctaaa atagctggtg acacaagcgg taattatcat
    51421 cctcatggag aacaagttgt ctatcctact ttagtgcgtt tggctcagcc ttggataatg
    51481 cgttatcctt taattgatgg acaaggaaac tttggttcta ttgatggaga tccacctgca
    51541 gcaatgcgtt atacagaagc ccgtttacaa aaaataacaa tggaaatgct ttctgaactg
    51601 gaaaaggata cagttgaatt tcgcagtaat tatgatgaaa ccagacaaga gccaacagtt
    51661 tttccttccc ggtttcctaa tttattagta aatggttctt caggaattgc tgttggtatg
    51721 gcaacaaata tgccacctca taatattgga gaagtttgcg aggcaattat tgctttaata
    51781 gataatcctg atttgcaacc gctggatttg cttcaataca taaaaggtcc agatttccct
    51841 acaggtggtt tcattttagg aacagaaggt attaaagatt attttgaaac aggtagagga
    51901 agaattatta tgcgcgggga agcagaaatt gatactttac ctaatgattc agaacagatt
    51961 attatccgtt ctatacctta tcaattaagt actacacagc tcattgatcg tatggttgaa
    52021 ttagtaaaag ataaaagaat tgaaggcata agtgatatcc gtgatgaatc tggtcgggat
    52081 ggaatgcgct tagttatcca agtaaaacgg aatcatgatg ctcaagttgt tcgtaattta
    52141 ctatataaac atacgcaatt acaggcaact tttggcgtta ttaatctctg tcttattgat
    52201 ggtgtacctc aaatagtaaa tatgaaagaa atgttacaaa atttcatcga attccgtcat
    52261 gatgtgattt tgcggagaac actatttgac ctgcgtaatg ctaaagatcg tttgcatatt
    52321 ttagaaggat ttaaaatagc gctggataat ctggatgcag tgatagctac tattagagct
    52381 tcacaaactc ctgcagatgc aagttctgct cttcaagaaa aatttgggct cactgaaatt
    52441 caagcaaaag caatattaga tatgcgtttg caacggttaa caggtttgga aagagataaa
    52501 atcgaaatag aatatcaaga attactgatt actattgccc gacttactga actggttgaa
    52561 cataaagagc tacgaatgaa cttaattaaa gaagaaacga gcgaaataca ggataaattt
    52621 gcagataagc gtttaactac tattattcca gctcctgcgg gtagcttcaa tatggaagat
    52681 ttaattgctg atgaaatggt tgtagttact atcactcatg atggttatgt aaaacgttta
    52741 cctgttgata cttataaggt tcagggaaga ggaggaaagg gcttaagtgc ttctaatcta
    52801 aaagaagagg attttattca atacatcttc gtagccagta cccattccta tatattactc
    52861 tttacagatc atgggatgtg ttattggcta aaggtttttg aaatacctga agctagtaga
    52921 actgccagag gtaaggcaat cgtgaattta gtaaatcttg ccgaaggcga tagaattaaa
    52981 gcgtttgtaa ctcttaagga ttttcatcct gaacaaaata ttattatgtg cacccgtaag
    53041 ggactggtta aaaaaactcc tctatctgct ttttcgcatc cacgttctaa tggtattaaa
    53101 gcaatcaaat taagagaaga ggatgaattg attgatgcca gaattagtga atctggtgat
    53161 gatatccttt tggctactca tgaaggaaag tgcaatcgat tcagtgaaag tgaaattcgg
    53221 cttgtgcgtc gtttttcaca aggtgtgcgt ggaattcgtt tgcgtaatgg tgattatgtt
    53281 atttctatga caattgccac tgctcaagat caacttgaaa acggtaatcc aaatgctgct
    53341 actattttag ccatcagcga aaacggttat ggtaagcgta gtccaatatc cagctattcc
    53401 tgtacccgtc gtggttctat gggagttata actctaaaaa ccaataaacg aaatggctat
    53461 ttagcatcat taatgctgac atatgatgat gacgatttga tgattattac tcaaggtgga
    53521 atgattattc gccaaaaggt ttctgaaatt tctgtagtag ggcgtaatac tttgggcgta
    53581 aaattaatca atcttgctcc caatgatgtt gttcgtgata tttctttaat tcattccgaa
    53641 ccggatgaag aatcacttga taaagaagtg gaaactttca aagcaaaagc tcccttcaaa
    53701 tttgaagaga aagctgctac ggacgaggaa gattatgaag aagaagatga agaaatggat
    53761 gtgattgacg aattagaaga agaagacctt gataatgaag aggataacga atagactaat
    53821 aaaaatgata ttaccggcag taattttatt actgccggta gcttgttctg taaatcaatt
    53881 tgttcctgca gagggagttt tgagtgaaga taactatgct attatcaggt cagatactct
    53941 attgataatt gtgagacctc agaattatcc aggtagttat caggaaatca atagccgttt
    54001 ctttccccta tatttcaaag tgaaaaatac ttccagccat aagatacatc ttaatcagag
    54061 ttcttttagc atcctatgca atgagaaaca atatgattct attcccttag attatgttct
    54121 ggcagattta gaacgacgaa tattgctgaa acaattcagc gatccttttg ctccgcaagt
    54181 taccaatttc ggtattacct ataaagagaa agaacaggaa atgtactatg aacttgtaaa
    54241 taatgccttt agttttggtg acatcttgcc tggagcaata aaagagggtt atctttttta
    54301 taatcgagaa atagcttctg ctaaaagatt cactatagat atactgggaa acacaatctg
    54361 ctttgtaaag taatcgatat ttttcgctat aaaattagcg gtattcatat atcatccatc
    54421 cttctttacc caatcctaaa aaaatatagt catttgccaa gtcaatatca taaactggta
    54481 cagttaaaaa gatattattc agttcttcgg ttttaagcgg attggttaca tccataattt
    54541 ttactccacc tcttcccaag gctataaaaa ggcggttatc tttcattgcc agatttaata
    54601 tttcattagt cattcttatc tgactgataa gcacgggttt tttggggtca tacacattaa
    54661 aggccatcag atttttccct gcgcctatcc aaattgtgtt tccatattta actaaacagg
    54721 aagcatctac aatttcgtaa tttaccccta aagaaactgg ataggattgg tcactaatat
    54781 caacagcaac taacccaaaa tcgttgagag caaagatata tgggtacgca gctacgaaat
    54841 ctttgattcc ccatttttca gaaaaagtag ataaaatttc gcgtgtgctt ccactgccaa
    54901 tattgataat atcaatacct ttataacggt ctgccacata taaagttgca ccttctcgag
    54961 ttaatttttc tcctaaaatt gtctgaggaa agaagattcg ggaaccagta tataagctat
    55021 ctaccaaaat tacttgattt gtaggttcgc tggatgctac atagatatat tttccagcaa
    55081 gtgtttcaac atcttcaata ggaaatgtgg aaaagtaagc agcttcaagt ttaggttgcc
    55141 aggcattaaa aattgagtaa atccaaatat aattatgact attggtataa agcaaatcat
    55201 tatcaaattg gatagaatat gcttgggagg atttaacata agccagttga tacagaaatt
    55261 tgttatctat cgcaaaagct gataaacaga ttaataaaca aatcgcactt aagaagatgc
    55321 gtttcatcgc tcctccagta accagtgaat tatttgtttg gagttaattc cttctgcctg
    55381 agctgcaaat gaaggtaata ttcgatgctg taacacattt attgctgatg ctttaacatc
    55441 ttcctctgcg ggagtatatc tcccatctaa agcagctctt gcttttgcag ataaaattag
    55501 atattgagaa gcacgaggcc cagctcccca agcaacccat tgtttaatct ctttagatgc
    55561 ttcatcagta ttgggacgtg attttctaac taaatttact gcatatttaa gaacatgatc
    55621 acttacaggg agagagcgaa ctgcttgttg aaaagagatt atttgctctc cagaaagctg
    55681 aggtgagata gtaggaattt ccaagccggt agtactatct gcaattaaaa gttcctcttc
    55741 catagtaggg taatctatat tgatgaagaa cataaaacgg tctaattgcg cttccggtaa
    55801 gggataagta ccttcctgtt caattggatt ttgcgttgcc ataactaaaa aaggtttact
    55861 taaagggcgg gtaacagttc cactggtaac agcatattct tgcattgcct gtaataaagc
    55921 agcttgggtt ttgggcggag ttctgtttat ttcgtctgcc aaaatgatat tggcaaagat
    55981 aggtccttta atatactcaa agcgtttttg ttcagttatc gggtcattaa ccaaaatatc
    56041 tgttccggtg atatctgagg gcataagatc aggggtaaat tgaatgcggc tgaaagaaag
    56101 ggagagtgct tttgctaaag aagaaataag taaagtttta gcaagtccgg gaaccccttc
    56161 cagtaaaata tgaccattgg caaaaagagc aatcaggaca tctgtaagaa ctctatcctg
    56221 cccaattatg acattgtgaa tttcattaag taatgcatct tttaaactct gcatcgagtt
    56281 tatatcttgt atcatttctt ctttagtcat ttattttttc tcctttaaaa acactaataa
    56341 aaagctctgg accaagaaca gcaaaagcaa ttatccccca taaactcatt aagccagctg
    56401 ttaaaatatg tccaaaagcc ataattgctg aggcagtgga ctttgtcaat ccaaatccaa
    56461 tgctgaaaat aataatcatc atccattcat tactgcccag ctgagccggt ggctgaggaa
    56521 tagcataaga tagatttata agtgtgtaac caaacagagt gatagcgaaa ggatataata
    56581 tcccgaaagc acgaaaaagc aaataaaaat atgttccatc cattaaaatt ccaaatgccg
    56641 ttaagagcaa agctaaaata agcttcaagg gttgatgttc aaaaatgttg agttcttcaa
    56701 taaacatctc tatagcagga ttgattctat cttttgcctt ttgtggaagc caggaaaaga
    56761 taaaagtgag catttttatc acccattctt tctgccagac agaagccact aaaattgaaa
    56821 aagctacaat aaacactaac atcaatagac ataataaaat aatcattgct gtgcttagtt
    56881 ctatagtagt aaaaggaaga acgattataa taaataaaat tgctaaggta tcaaaaacct
    56941 tgtccacaaa aacggatgga agagctttag cataaggaat atcataattc tttttgataa
    57001 accaaccgcg ggcaaaatct cctgccctga taggtattaa atagtttagt aagttcccag
    57061 ccatagaata tagccaagtt ctacaaattc cagggtttgc ttgagcggga agtaataatc
    57121 gccagcgtaa agaacgcaaa aaataagcga aaagataagc aagagaagca aaaataaccc
    57181 aattataatt aactttggca atctgagttc taatttcgct gatcggagta taatataacc
    57241 aggcagcaag taatattatg cctacaatta gcccaattat caatgtatta cggtttttat
    57301 gtgtcattaa ccgttatccg gagtgaacag aagataataa tgatgtgccc agtgtttttt
    57361 aaaactaggt ggtgcagttt tttccacaaa accataggaa agcattcttg atataaaatc
    57421 actattttct tcctgataat aggagagaat agagagtggt tctctgtttt tttcctgtat
    57481 ttcctgcttt ccgaccaatt ttttaaaaaa gagttccttt ttcattccga tatcaggcca
    57541 gggaggacaa tcaatataac cttctttttg tagtaaccac ttgttttgtt tcatcagata
    57601 tttgatagta gccaaatcaa gatgagcagg atgcaaataa gggtattttt taggggaata
    57661 atccttcatc tgcattttaa aacctattcc atcacgattg ggaacgcaga tcagaattgc
    57721 ctttttacat actctacaga attcagcaag gaaacttcta atattttgag taaaccaaag
    57781 cgcagaaaag ttgaaaccca aatcaaaatg tttatccgga taatcaagtt ttacatattc
    57841 agggttatat ttgatatcaa tatgcaggcg taattcttgc cataattcat tgatttttgt
    57901 aattctttcc agatcgtgat cttccaagga gacctttatt ccctgtttag ctaaatttac
    57961 aaggtttatt ccgcttaccc cagtaaaacc aaaacaaggt gtttccaacg cagattgaat
    58021 atgatatact tcacaaatag aatctaataa ttggttcagg ataactcgct cgtaggaaga
    58081 gcccaatcct tcatcaggat tatcaaaata tttagcccaa tcgttgataa tgggaacggc
    58141 catcagtgct tctggtaatc ctttattgtt atttcaaatt caggaatagt aaaatgaggt
    58201 tttatacctt ctctttccat aatatcataa tatgcggtta catcgtaaaa ccagcttttg
    58261 gatataagct caaagcggct gatccaaagt tcatttttaa gcattctggc aagcttttca
    58321 ccccaagcaa aaaatatcct gtcaaaagtt aaagcaccag ggtagttttt attatgtaac
    58381 tgacgggaaa taaaatctac caaagcctgt aaacgaactg gttcacgatc tgcaattgta
    58441 agggttaaac cacttttcca ttcgttagtt gccaaataga caaaagcttt taccaaggca
    58501 tcaatgtgac atagatgaat ccaaatcgga cggttaatta atggaaacat atatttatct
    58561 acaagcttaa ccaactgata aggaaaacca aaatcacctt ctccataagc aatactgggt
    58621 ctaataatag atgctttcag tccatttaat attgctcggt tgattatgcg ttccgattca
    58681 attttagtgt aatgatagta gttgtcagga tttttctcgg tttcaggtcc agcaggtaat
    58741 tcttgaggga tagctccaaa aacacctacc gaggagcaat aaatcaatct tgcttctttt
    58801 gccagacagt attctaccat ttgtcctgta ctataaacat tgcttcgtaa atatgtttct
    58861 ttcgggaatt ttcttcctcc gcgtaaagct ccaatatgta agatagtatc aaattcattg
    58921 gtaaaaagaa attcctttaa agaagctgtg tcagcaaggt ctatttcaat gatcctaaga
    58981 tcctttccaa attcagaata tcggtttgga tgtgtttggg gacgcactaa tgcagttatg
    59041 ctatagtcag gacaggtatt cattaattcc cttaaaacat ttctaccaat gaaaccagtg
    59101 ataccagtta taagaagtct atccaaaaat cctctttagc aaactatgtt tatgatgttg
    59161 gtgatgatga gaatgatgaa cttcaacata ttcatagggg atataatctc cattgtcaat
    59221 acaggaagga tataaataaa aaagcagatt cgcagtatga ttatcgatca cttcctcgat
    59281 tttatttttt gcttcaaaat aagaatagta ttttccccgt aaatgatcat ccgaagcaat
    59341 aaaatgtgtc caacccttat ttactaatat ccaggcagtg gatttaactt tttcaccata
    59401 cagaccaagt aagctccctg cactagtttg tatataaatg ttacgatgaa taaggtcttc
    59461 agcgcgtgag ggcttttgca taatactcac atatcgttct gcgtgtgcca aaataggttt
    59521 ataaccagct cgtaataagg ggtagatgtt attgtaaaaa tcactgggta gaccatttaa
    59581 gtcactttcc aaaaggatat aattgctttt acccatagtt agattatgtt gttttatatc
    59641 agataggatg ccgggttgca aatatatttc aaatccaggt tgcaaatcga tatctatacc
    59701 ggcttttata acttcattaa ctagcaggtt gaatttatca tcgtattcac ttcgcgtata
    59761 ttgaaaatgt cctctaaaat aatgagaagt taagtaggct ctactaattc ccccttctgc
    59821 catcaaagat atctggctga gagaatcgtc cagtgatttt gaaccgtcat caatattggg
    59881 taataaatga gtatgaatat ctaccattgc ttaaggtaat ctttccttaa tcatagctac
    59941 gaattctaac aaaacagaat tattgggaaa gggtttaatt atctttgcgg tatcttctaa
    60001 catattctct accattttac gtgctttatc aaaaccaagt aagccagtat atccggattt
    60061 tgaaacagga atatactcat catctaagcc atcgcttccc cgagagtaat ctgcttctat
    60121 atcttcaatc atttgatagg caatgcctaa attcacagca taagtattca taatctgacg
    60181 agtttcttca tcagcattac taagcaggca agctatatct gctgatgctt gtaacaggga
    60241 accgactttc ttcatatcaa tataattcaa ggtgttaatt ttcataactt tatgacgact
    60301 ggaaagtgct actacctgcc ctccaatcat accataactt ttagctgcat tggatagaat
    60361 ttgaatggat ttaactgcct gttccgaatt ttgaaattct cccaaaattt cgaaagcaag
    60421 agaatatagt gcatctgcag ctaaaatagc tatggcattt ccatatttta tatgcaaggc
    60481 aggttgacct cttcgctgag ttctattcat tatacaagga aggtcatcat gaattaagcc
    60541 gccgttatgc atcaattcaa tagctaccgc agcttttact gcatctggaa aggcacggtg
    60601 ttttttaaaa ctggtaagca tttcaaacat agaaagaagc aggataggac gccatctttt
    60661 acctccggga ataacagctt catacatagc ttttttcagg ttggcagcat attcatcttc
    60721 gaatgataat gcctgttcca gtccttgatc aataaactcg aaacgtgttt caaaatactt
    60781 ttgtaaaagc aaagcactct cccttttaaa tatcacttag tttaaaataa ctgtatttta
    60841 ctcttccttt ttatatcgta ccatactttt gtcaagtttt ttctcccttc agtgaattat
    60901 aggtgtacat ataatcgaag tatggaagct aacaattaat gtaaagccta tagggtttat
    60961 ctatccaagg attgttatag ctggttatca acccaaaatc cactcataat tgagttttgc
    61021 aagtgtatga gtggattatg agtgacaaca aaaattgcaa taagtgaata aaaagagtga
    61081 ttcctttttt atatcctaaa tcttaatttg aaaacctatt gagatgtttc ttcctggctc
    61141 tggaaatccg tattcgcatt gatagttttc atccaggata ttttccatcc caacagacag
    61201 ttctatttgt ttatagggaa tagtaataag cagatcgtgt ttccaatagg gatccagatt
    61261 atgatatctc ccctcatcgt cttgagagag gcgttcatct ctaaaagagc tgtcgaaaat
    61321 aaaactaata ttgaaaggta gtttgattcg ttgtattaat tctactgaat ttttgggtgt
    61381 ctccgttaaa taataatcac tattttcctg atagctgaga taagcgtaat ttacactcga
    61441 ttgccaaaat ttaagcaaag agatgtccat accaatttct gcgccttgtg attgaacttt
    61501 aaatatattt tcatagcgtc ccaaatacat atcaatgagg tctttggtat cgttatagta
    61561 aacactgcag taaattgaga gtttattttt tagcaaggct tgattgtggc ttagttctac
    61621 ttttagtgag tgttgtggtt tcaaagcagg attaccttta ctttcagaat aaagctgtct
    61681 taaagttggc tgagaagtat tccgtccaat ggcaaaagag gttttactat taaaagagct
    61741 gtcgtaagta attccaactg ctggttccaa ttgcggttca aaatcattat ggtatttatt
    61801 tattcctgca cttaagccca atccggttgt taacaataaa tcaggataaa gaaagcattc
    61861 atattgactg aagaactgat gtagatggac aggtgaataa gtccattcaa gatatgaacc
    61921 attatcctta cgacgattta gctgaaactc ataatgataa ccaatatcca gttttctgtt
    61981 acctaaaaat accttagtta aacgaggatg tacaccaaaa gcttgattgc gcatctgtga
    62041 atctaaagtc atcgtttcat aagtgggatc attatattcc agatagcggt ctgcgctggt
    62101 atcataagtt aattgcagat tcacaacgct gctttcattc atttgaaatt tagtccacaa
    62161 gccgctgtaa taacgcatcc aatcctgata ctgtctatag cgatgttcat atatggaact
    62221 ggggatattc tttttatcca tcgtgctata cccaaaatcc aagccgatat tatgaaagtt
    62281 taacaaatca ctttcccatg aaccggatag attgtataaa cacttatcgc tattattgcg
    62341 gattcttcca ttttcggaac tggtaggaat aaaatcctta gagagattaa agccgtcagt
    62401 ttgttccctc gaaagaccga ttctataatt ccaactatca aaactgtgag ctgaggatag
    62461 tttaatttca ttagtgttgt tgcgtttaaa aattgtgttc agttccagcc agtaattttt
    62521 aggcggggct ttggtaataa tattaactac cccgcccata ttatttgttc catataaagg
    62581 ggaagcagga ccttttaaaa tctgtatttc ttcaatatta agcagggaaa gtttactgat
    62641 atccacattg ccaaaatatc cgttattcaa aggtctgcca tccaccataa tttttatttc
    62701 atttttgcga aaaccgcgta agcgtaagtt gctttcatcc ttactacctg ttgttgcact
    62761 aatacctaca ctgttttgca ttgcctcccg caaagaaagc gaagtcttaa catcctcact
    62821 atctattcta tgtaaagaac caattgcttc agacggtttg tctacaatta cataaacagt
    62881 tggcagatta taggttctta gtgtatcttg ttttgctgtc ccaaataaac taagagcaga
    62941 cagcaataac aacagcaata gtatatattt ctgcactttc actttcgtag ctacaattac
    63001 ctatgttctt tacagtcggg ttttaataat cagttaagat agtggaatca tagccactaa
    63061 aactttctgg aacagtaata gttatgtctt ttttatcatg cggggcatta gcaaaactat
    63121 tatcttgttc ggtagaattt gtcacttcaa tgcttgctaa ttttttaaac ttcttcaacg
    63181 aacctggtaa gagttcattt aaagttgtaa aagtagttac ctcagaatcc attaagtaat
    63241 aacccacagc aatttgttct ggcgtataat ctttgctata gccatcaatg cttattaaac
    63301 ggatattact gctatcggat aaagttattc cttggatcaa atcacttagt ttgatggcgt
    63361 cttcattaac ggaattccag ttttggatag aatgaattgt taaaccttta agctcaacag
    63421 tatttgtttt acctgcacta ttaacatcta ctttccggta taaattaaat ttggttgtat
    63481 tcttaacttt gaaagcgccc ggaagaccaa gctctgaggg aaaccaagtt ctattattat
    63541 cagaaggaac aataaaaccc tctttaaaga agtcccaagt taaatcatat ccggcataag
    63601 atgaattacg agggctaaaa ccatcgctgg caataatttc ataagcgaat agatttctga
    63661 aattcaaagt gtccgaagca ttgggatcaa ttaaatcacg caaaacagtg ttgaaaatga
    63721 attgatccag tttgtaaccg taaacattaa cctctgtagg attgtcatcc ttttcactgc
    63781 aagcaaacat aaacagggta aggatagcga tcaagatcac gctaattttt ttcatttttt
    63841 cctccatact taattataga ggcagacagg gatgaaaatt atatttccgc tgttgccttt
    63901 ccaaatacgg gaggcataaa tgtttccatt tatacccaat ggtgcatttc catactttca
    63961 agaagctaaa ggataataca ctcggctttt ttcacaaaaa tcaaagcaat aatatttgtc
    64021 aactccaact ttaatattta gcacgcagat tacgctgatt gcgcagatta taacctgatc
    64081 caaatattcc ataaatcgaa gcaatccagt tatttatgtt caattgtttc atttaaggat
    64141 ttctggtcat ttggattttt tgcattttga gctattatat ctaataaaac tataagaaag
    64201 gatgaaaaat gcgaaaatac ccgttaatag tcattttact cttgattttg gtagaactat
    64261 ttgcctttga tatctatttt ccagatggaa aattactgcc tataaacaaa gagcaattac
    64321 agcaagaaaa aatggtaaca tttaatactc ttagggataa ggatggtgct caaaaagaag
    64381 aaaactggca aggaattgct ttaattccct ggcttaattc cctaaccaat atcccctggc
    64441 ataatttgaa aattgaaagc aaagacagtt actctttaac ttttcaccgt ttggaactaa
    64501 acgaaaacaa tgcattcatt gcaatgatgc aagatgggga agagctatct gaatataata
    64561 tgcgcttaat atttacaaat tacagggaaa atgcatggat gcgcaatata aagtgtttaa
    64621 gacttttgga ttatattcca gttcccaaac ccagacagat attcttttgg gatcagagct
    64681 ttgccaaaaa tggaattgat tttagttctg gatatgttcc tattggcgat ttgatgctta
    64741 agtgcttttt tcaggacagc ggcgatattg tttttgtgga tgaacagttt cgtaaattat
    64801 gcttaaatta tcctcgtgaa ctgggcagtg tctttttaca cattgatgaa aaaggtaatt
    64861 atactttggc tccggacaaa agctcacacc taaaaatatc atcccagaca gatttatctc
    64921 aactaattta tgtccagtgt ggccaagtgg cttatattaa agagaattat gttcaagaga
    64981 ttaggcaaat ggcaaaagtc ctaaattggg attgggaaag tgattcttta catctaatca
    65041 aagccagcaa agaagaaata aaagcaggcg ataagccaaa ggagataaat gctgattgct
    65101 ggctggaatt ttaaaggtgc aaataactct cgcactcccg cactccnnnn nnnnnnnnnn
    65161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    65221 nnnnnnnnnn nnnnnnnnnn nnnnnntaat acataatata ttgttgcaca agcataaaca
    65281 taactttatc gcaaagttca tttactgcat ccagttcatt tttgccttta gcagcatcag
    65341 tggtaactgc cacactttta attccggtta aatttgtatt gtaaatactt tttccagtgc
    65401 gggaatcaat cagttcaata taggcatcgg cgacggctga atattgtttt aacaaaggca
    65461 gaaattcact attgcgagtt atgatgttca cttcaaaggt ataatcactc tcattttggt
    65521 tggttgaaat ttccaaatct aaatcctgca gttttttcag caaaatattg cgatagtcgg
    65581 tcccagaagt attattaaaa ctatattgca caaatgcttt aggacgatta acattcagtt
    65641 ttaaagtggc aggtaaaaat tggagattgc caaacattgt tttaactata ggattttcta
    65701 tacccacaag ccaaaaatcc ttatccggtt tggcttctat aaattgagga gtactgaaag
    65761 aagttatgcg gtttatgatc aattctgctt ttccattttc gtctgttaaa ccctcagcaa
    65821 caatttcgcc tttaccgctc aaaaaattga agcaaacagg aaaattattt acgggtattt
    65881 cttgttgata gttaacgaag acagcaatgc tttttctttc tctttgtttg gctgtgagat
    65941 caatctcttt ttccgcatag tttattttca ctttttcggt taaatggttc agacgaaaga
    66001 aaagttcatt ataaagatta atctgcttat tccgataact cgtggtcaaa tccatatcgg
    66061 tataatccac aatcagttcc agtcctttta agagagccga tatgccggaa gttaaatttg
    66121 tttgagagag atcaaattcg tttagtaaat tgagagcttg agccgttgct tgctcacatt
    66181 gattttttcg ccaggcataa tattcacttt tggaaagtcg gtaataagcc caatagtcat
    66241 ttttggtttc ataagtgcca accagttgca ggtctctaat tttattatta ctggaactgc
    66301 ggatgctgct gattaattct gaagaaggaa ttccattggc ttctgtttct tttaaagcaa
    66361 tgtcggaatc aatttgaacg ctgatttggg tggagatttc acgaatagcc tcatttctgg
    66421 caagttcttt atagttgggc gcttttttgg gaatttttac taccgcacta taatatccat
    66481 tatcaaaggg ttgttgcaaa gtccattctg gctgtttttt gcctacgctg caagctgaaa
    66541 gtaacagtaa aagaatatat aaaccgcgct tcattagctt aaatatccgc aaagcttatt
    66601 agctatatca gttccggttt gtttcagcgc ctctttaacc atttcattta tggaaggggg
    66661 aaagggttct gctttttcca ttagcttttt tatgttgtag cttagggcac ggtcgtctcc
    66721 ggaaattttc cttgcccagg tatcgctcca agaattctta atttccacag tttcctgctg
    66781 ttttattttt cctgtttcta cttctaaaat gctataggaa gtggaaatgc taacatttgc
    66841 agttttagtg aatttacgat agcggcaaaa gacttttccc ttgatttcgc gttcctgagt
    66901 aataccatct tcatctgtat attcttctgt gcggattaca acttcggttt cgtcttcact
    66961 ggtaatgcta acagtccgtt caggagtaac atttatttgt aaaatgcgtc cggttaagat
    67021 ctcatcagca cccaaaatct ggcccaagcg aaatgaacct tcattattaa ttaaaccgga
    67081 agcggtaagc tgttgttctt tcataactgc ttccagttgg gttctatcta tgatttccac
    67141 aaaatcactt tttgcggcag atgaaattaa caaagagaca atttgatcgg tcaatatatc
    67201 actgacggct ccatatatgc cagaagtatt gcttttatct tcaaaaggag ttatcgctat
    67261 tctttttatg gcaagtttac ggctttgttc atatcttaag tcagcatccc gatagttagg
    67321 aataagtttc agcgcttctt taaattccag tgcggctttc ttttgcgtat ctttatcatt
    67381 actcattttg gcatataaaa tgcctgcttg ataatgtgcc tcagcggcat tttcttggct
    67441 ttgtttcatt tctgctgcat aatcaggaat gtcaattaca actttataac cagtataggg
    67501 atttatcaaa ggaggtagcg attggatttg ctctgccaaa ttttgcagag aagtatattc
    67561 ctggtaaatt tgttcccact tatttgtttc gttgctttgc atcagttttt ctatctttct
    67621 ctgttgcgcc aaacgataat tttgccagga ttgcaccaaa agttcctggg ctttaggatt
    67681 atccgctttt ttgctgagtg ctcttatcgc ttcctgagtg gcaatttcgt aatttccctt
    67741 ttcataattc ttttgtactt ttttagctaa ccgcgcattg gaagagcaag cgctgattat
    67801 caataaacaa caaagaagaa cgggaaaaag ccattttttc attaaaactc caaaattcat
    67861 cgtttattct acacaaaaaa acttctatgt atgcctatgg gtttatgtca agcggatttt
    67921 ggtcaagggc tgataagcca acagattata cgataagcaa agtaaattat aaatagaata
    67981 taaacaagat tatcaggatt caattattgg aaatagatat t
//
LOCUS       DAOSTH010000005        60757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_543_length_60757_cov_11.285427, whole genome shotgun sequence.
ACCESSION   DAOSTH010000005 DAOSTH010000000
VERSION     DAOSTH010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 60757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 60757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60757
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_543_length_60757_cov_11.285427"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(190..1281)
                     /locus_tag="PLB65_01860"
                     /pseudo
     CDS             complement(190..1281)
                     /locus_tag="PLB65_01860"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
     gene            complement(1699..2883)
                     /gene="ftsZ"
                     /locus_tag="PLB65_01865"
     CDS             complement(1699..2883)
                     /gene="ftsZ"
                     /locus_tag="PLB65_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOQ77301.1"
                     /translation="MIEFDEKPSQIGTNIKIIGVGGAGGNALNTMIENNLYGVEFIAA
                     NTDSRDLAKSKANMKLQLGKKLTRGLGTGANPEIGARSAEESKDDIKSHLDGADMVFL
                     TAGMGGGTGTGAAPIIAKIAREMGILTFGIVTSPFPFEGKKRAENAMYGIKHLCEFVD
                     TLLVIPNSKLCEIYSTLALEDAFHQADNVLYEAAKAVSDIINVTGLINVDFADVKSIM
                     QNMGYALIGSGLAEGDDRAINAARAAIDNPLLTHISLQGCQSLLLNITAGHDILMSEF
                     DEVSNVIVSETGKAANIIMGIILDDSMAGKIRVTIIATGLDKEGKTHLIDFPGLPNIE
                     ETTEENVKIVKESDEEIKDIFARLNINKPATKEEPKQVTNETGRVPPLRTDVPSFLKA
                     LD"
     gene            complement(2895..4166)
                     /gene="ftsA"
                     /locus_tag="PLB65_01870"
     CDS             complement(2895..4166)
                     /gene="ftsA"
                     /locus_tag="PLB65_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOQ77302.1"
                     /translation="MKQSIITALDIGSSNVRCIIAKEIGEGRLEILGIGESPSEGIEC
                     GIVKDIQALSGCVAKAISTAENDAKIAATNIYCNITGDHIRTQIGDGRISIPSEIPNE
                     PGEITQEHTEQVINDAKNSVKIQKGLERCRILHGIPHNFIIDNQDDIHNPVNMNGFHL
                     IARVYTILAELTPLRNLNKCIQLAGYDIDPENFVLNHIAVAESILSEDERRLGALVLD
                     IGGGTCDLSIFNRGTLEKILVIPMAGKNITEDLAIGLKTTLGNAEYIKIEYGNALANS
                     VDQTEEIDVEGISGRASSRKTRYLVSNVIQHRVEEMLSLCYTKAKDYYTPELVTSGII
                     LCGGTAKMKNLDIVLSESFNLNVKTATPDLSRLNGMISRLEDPAFATVIGILYYASGK
                     NTEPVTKGFALPNIKSTKIVDKIKKILRDFT"
     gene            complement(4163..4930)
                     /locus_tag="PLB65_01875"
     CDS             complement(4163..4930)
                     /locus_tag="PLB65_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HOQ77303.1"
                     /translation="MELNKGTRKRRGNSRYYLFFFFALIGVAIIGSGIWFGLTNIDLF
                     KFQKMQISGNKVIPDTLFYKITSPYIGMNLFTVPSSEIKAKLNTLSRVKNVKLHKRLP
                     DTIKLDITERKAAIYLKTIEGDLYPVDSEGVVLIIYTPVYKEDIPIYSTYLSNNQIKP
                     GIKLKYSGLQKVLQLHQRVVKEAPDFLPQISEYYLIDNTINIIDAKTGTRIIPSEEDF
                     ATQISRYRFVQENGNINRNSVVDLRFKNQVVVKAGNK"
     gene            complement(5030..6406)
                     /gene="murC"
                     /locus_tag="PLB65_01880"
     CDS             complement(5030..6406)
                     /gene="murC"
                     /locus_tag="PLB65_01880"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOQ77304.1"
                     /translation="MLGRTKKIHFVGIGGIGMSGIAEFLHNQGLVITGSDLKKSEITA
                     HLEALGIKVEEGHRPENLGDADVVVKSSAVTDDNPEILAAKEKKIPVIRRAEMLAEIT
                     RMSFSIGISGTHGKTTSTSMAGSVLESAGLEPTIIVGGKVKNFGSSNVMGSGKYIVVE
                     ADEYDHSFLALTPCIAGITNVDVDHLDCYQDLDHIKSSFIEYANKVPFFGSVIACLDD
                     PGVQSILPFINKRIVTYGFSRQADVQARDIEMQGFKSSYDLSYKEYKLGRINLNVTGK
                     YNIQNSLLAASIGLELDIPFTAIQLGLQEFNGVYRRFDMKGEAAGITVYDDYAHHPTE
                     IKATLEGFKDSVKRRIVVLFQPHLYSRTRDFYEEFGKSFFSCDCLILAPIYPAREQPI
                     PDITSKLIADAAIQSGHHQVHLVESNSEIVSRTLPLLKEGDILITMGAGNIWKYGEEI
                     LGKLKELC"
     gene            complement(6406..7488)
                     /gene="murG"
                     /locus_tag="PLB65_01885"
     CDS             complement(6406..7488)
                     /gene="murG"
                     /locus_tag="PLB65_01885"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOQ77305.1"
                     /translation="MKFIFGAGGTGGHITPALALADELQKYNHDILFIGNRNSIEESL
                     CKSAGYPIRYIKVQKLYRSFKLENLVFPFFLASSIIAAISILKKEKPQAVICTGGFVA
                     GPTAIAASLLKVPLFFHESNSYPGLVTRKMAKKINTIFISFENSKKYLVKAKTVNYGI
                     PLRQSKISSTQKTFELSSLGLDNNKPTIIVSGGSQGSIAINTVVSSAIPVILSMGYQI
                     IWQTGKTSYERFATKHKNTEGVYMFAFSPELPKMLSKCKLAITRAGAMTIAELEENCI
                     PAILIPLPTAAENHQYYNALAQQEKGVAMLLEQKDLSLLTLVESLKKMSENLAYYQDN
                     LFSLPKNQAAEKIVHYIINYLQNKGE"
     gene            complement(7485..8624)
                     /locus_tag="PLB65_01890"
     CDS             complement(7485..8624)
                     /locus_tag="PLB65_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HOQ77306.1"
                     /translation="MKRSKIQSYIVSFDKVILITYCLLCVIGLVVMLDISSVQSSMRY
                     FYRQLFFMIISAIFAIVILYTLNLEKLRVLSPYIVYLTILLLIIVLVKGNTVKGATRQ
                     INLGFINFQPSVLARLALIFYFAHILDKKNEELVSSTTGSFLGNFFALIVVTGVTFLL
                     IIMERHLSTLIIGGLTLYGMLIYAGAKKRVLITIAIIGIIAGCLVLANGADYRKGRLT
                     TYKKFCLFLKPAGEIKIEDNDYQVKESLTALSSGAIIGTGMARGRAKHYYLPEARTDY
                     VYTVIGEEWGFVGALIVFGIHCFLFFRCFWMAHSQENRFLEFLGVGLAMNIFCNVLVN
                     TGVAMSLLPPTGNTLPFISYGGSALLIDSIALGMLLNISAQRRYV"
     gene            complement(8611..9963)
                     /gene="murD"
                     /locus_tag="PLB65_01895"
     CDS             complement(8611..9963)
                     /gene="murD"
                     /locus_tag="PLB65_01895"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOQ77307.1"
                     /translation="MIEKNKKYGILGLARSGIAAAYKLKELGVIPFLSELRPETKIPN
                     AENLKKDFPCEFGGHTDRLLDCNCLIVSPGIPLNTSILQKARERKIELISEIELGYQI
                     KANDSKIIAVTGSNGKSTTVSLIYHILKSLGKKSILAGNIGDPFCGYSIEKPGLDYIV
                     LEISSFQLDLIDTFKPNVAVLLNITPDHINRYNTFEDYALSKVNIFKNQTATDTAVLF
                     MDSPEIMLYTKNIKASKLYFSLHQIETAYAYPVDNYIQLGPTTKVPLSNLEIRGPHNY
                     ANAMAAMLAVYALYPEPAKIAEAITGFKPLPHRLEFVGSVNGISFYNDSKATNTDSVR
                     SALHSFDKPIRIIMGGSDKGESYSTLTSDMQKIVKKAYLTGDTSEKMYQTWNGIIPLE
                     CISDFEDCVTKAYNDSLPGDIIVLSPACASFDKFKNYEERGEFFKLVVNRIIKENEKK
                     "
     gene            complement(9978..11081)
                     /gene="mraY"
                     /locus_tag="PLB65_01900"
     CDS             complement(9978..11081)
                     /gene="mraY"
                     /locus_tag="PLB65_01900"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOQ77308.1"
                     /translation="MFYHLLYPLAKYSIVFNVFRYVTFRSIGAFITSLIISLLVGPTF
                     IKMLRKRSAVETIDEDLPEKHRLKQGTPTMGGIIILFSLLISSLLWNVLTNPAILMMY
                     LATVWLGILGFLDDYLKNFVKAKKGLIPRYKLLGQISVGLIITLAIYYSSSVTDNITA
                     LQIPFFKNLIIPLGWIFIPFAVVFITGTSNAVNLTDGLDGLAAGTLAFSFLGLGVMSY
                     LKGNYIVADYLNLEFLSNAGELTVFISAIMGTLIGFLWFNSYPAQVFMGDTGSLTLGG
                     IGAVISILLKEEIFFIIIGLIFVVETCSVIIQRSWFKYTKHKYGQGHRVFLCAPLHHH
                     YELRGIHESKIVMRFYIVAAMLVAMGLSTIKLR"
     gene            complement(11081..13930)
                     /locus_tag="PLB65_01905"
     CDS             complement(11081..13930)
                     /locus_tag="PLB65_01905"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HOQ77309.1"
                     /translation="MINKIINALKQNSLLIETANLEQLQNWQGKVQTDTRKLQPGDIF
                     VCIKGTNYDGHNFIPSALNKGAALIVGQEFDNITFPYLKVKDSRKAAAIIARIVLLPE
                     KIPFTLIGITGTNGKTTTSIMIYEAIRDLGFRCGWIGTLGYYIEGRTFPSERTTPDII
                     ELNEIFAQMIDERVNYVVMEVSSHSLSLNRVYGVKFDYCLFSNLGRDHLDFHLTIENY
                     GQTKMSFFEQGAKDGSVSIVNTGDPFGIRIKEMLVEKHAKVLSLGSEDADFRFADVQT
                     DINNSSFRLQSDKDEIYIISRLIGNFNIQNLGLTALTLYAMGFKPEEIHKSIKDIPPV
                     RGRMEKVENNYGIGIFIDYAHTPQAIENVLEAGEDLPHKRMLCLFGAGGDRDKGKRPL
                     MLKAALNHSDVVIISDDNPRSENPEAIIRDIVSSSDIFLPWWIIRDRKQAIESIIRFA
                     QKDDIVLICGKGSENYQEIEGVHYHFDDLETTLEALAKRDTKIKDDAEFILPVDQTLM
                     KLLFISNWNPEPSGYKEPVYYKYLYTDSRILKPESIFVAIQGEKFDGHNYLKEVLAPK
                     ENCAIGEKSWSEESPIAEFQYFQVNNSLEAMGSICRKYLALFAPHKIALTGSTGKTTS
                     KELMAQILESSAPTLKTLANENNMIGLCKTILRIKPEDRYALFELGTNHFGEIASLAD
                     VVDPEIGIILNIGPSHLEYFKDEDGVFQEKIALFNRPLALRLYPGDDSRFQYFAKQGL
                     SIGFNENCDYRITQHQILDSSQSFYLNEDLWIIPYGAPYFALNAAFAIVLCLKLGISK
                     KDIQSALNSPIYLELRNQVVKRGTGILILDCYNANPYSMQKALEYWQTLSSEQPHYAV
                     LGDMLELGDSSVMYHKMIAAMLSEMRYEKLITVGSFSRLFHPDRENAILHYNNVEELI
                     ASGNLRTFPNNAIILVKGSHSIQLERIIPILKGEN"
     gene            complement(13927..16101)
                     /locus_tag="PLB65_01910"
     CDS             complement(13927..16101)
                     /locus_tag="PLB65_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HOQ77310.1"
                     /translation="MKSRFYFLVVIFGLASIFWVAYLFCIQILDPFHLAGVRRMRYTP
                     KKEILIPRRGSILDSQGNLLVSSISYYQIDIDRSSVAMWAKDNNLTLEDAFAKYAELI
                     AAHTSLNKDEVLKRLTIGNKNTSVQISNKISEVELDKLIRDFKSKEMIGLIHSFASMR
                     RIYSKDILAARVMGSVREVSDGYDPATHSKSLYQLSGVCGIESTYDKYLSGNYGWKEI
                     VLDANHRPVPYPNLHSKKPQNGYNVYLTIDSKIQEIVENALFEGMEQYSAKNAGAIVM
                     DPNTGKIIAMAGVSRDDRIEDPNLVRVKTNIPVSFMFEPGSTMKPLTMLPAIEYKLVK
                     PTETFQCGRYQTGRRVISDTHQYGNLTPREIIVKSSNVGIAKIAERIGPTRLYEKFIA
                     LGFGQKTALNMTGESSGMFAKLQNWDGYTLHSVSFGQGISTTALQLATAYCAIANGGK
                     LMKPIIVDTIRDEEGKIVEQFEPTVLRNVSTPAATDTIKSYLQGVVDNGTAKHIKMDY
                     IQVGGKTGTAQKNITGTKGYSEGKYSSVFVGMFPIEDPQMVIVVFYDEPAPGFHFGST
                     SSAPTFKKIVENILFMPDCQILPYNERLMQTSLTMPKLIGMHLFQAERTLNYYGFQYK
                     VEGPDSASVVIDQYPKEGVTVDPHYPITLKIGPSADKKAPVREAGIMPNLIGLSLREA
                     LKQASAHGLAINIRGSGIVRSQSILPGSRILEGSKCHLEASL"
     gene            complement(16103..16459)
                     /locus_tag="PLB65_01915"
     CDS             complement(16103..16459)
                     /locus_tag="PLB65_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77311.1"
                     /translation="MRGKILILVSLLGVVFFLSFYNNNRIVQYTRQISELEKTYYAEK
                     NINTELLVELDDLRSGRHIASLVRVELSNFIPDQQAGKIIYVHEPSAKQDKKTYCIID
                     LLTTKAEAKNIQVLLD"
     gene            complement(16441..17439)
                     /gene="rsmH"
                     /locus_tag="PLB65_01920"
     CDS             complement(16441..17439)
                     /gene="rsmH"
                     /locus_tag="PLB65_01920"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOQ77312.1"
                     /translation="MIFHTPVMAEKCISYLNLRAGKIYVDATTGGGGHSLAMLKSEPS
                     IRLYCFDQDMEAIEEASKKLSNYENVELIKANFQQLRTELAYRKVKGIDGILFDLGVS
                     SHQLNNENRGFSFEKDAPLDMRMDSSQEYSAYDAVNELDAKELARIFRDYGEELNAFR
                     IAKAIERSPKPVNTTGELTRIIESVVGKGTKASLKTKVRIFQALRIYVNKELEVLAST
                     LKDAINLLNPKGRIVVLSYHSLEDRIVKQEFKRAEQECICPPRSLACNCNHHKQLVIL
                     TSKPITASEEEILANPRSRSAKLRAAEKVKEENKEEKTYRKSNRQKTQEEKCVAKS"
     gene            complement(17436..17876)
                     /locus_tag="PLB65_01925"
     CDS             complement(17436..17876)
                     /locus_tag="PLB65_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MraZ"
                     /protein_id="HOQ77313.1"
                     /translation="MSGEFLGIFENSVNKQRVIIPAAFKKKFSEESEKKVIVTLGPND
                     TIAIYPLDNWNETLERLRIGDEHSNKLRTQLIDFAMTEQELEGPGRIRILERLLNEVN
                     ITDSVIIKGEGHYISLWNPKVYNEVRASKLNQHRKEFTSEDYQL"
     gene            complement(18249..18500)
                     /locus_tag="PLB65_01930"
     CDS             complement(18249..18500)
                     /locus_tag="PLB65_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77314.1"
                     /translation="MLSNIVRSIYNNPLLYIEPTKFKGNNSITFMLFIKNQSSNFLYE
                     NGHSILKIEISLPRISAKSDYFEEAPKKYFPAQNIAVSL"
     gene            18654..19136
                     /locus_tag="PLB65_01935"
     CDS             18654..19136
                     /locus_tag="PLB65_01935"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOQ77315.1"
                     /translation="MIKLSELPVSYGKVHLPAKVALVVTQKPTSGYNLITIEWFMRTS
                     LQPPMFAISIGESRYSHECLQANRYFNLVFPSLKMKQLLSLCGSNSGRDMDKFTVAKV
                     EYIQGKLNKLPVLKDAVACFECEVVSEIRSGDHTIFIGQVHYSWQNEEEELFYYQEKL
                     "
     gene            complement(19150..19797)
                     /locus_tag="PLB65_01940"
     CDS             complement(19150..19797)
                     /locus_tag="PLB65_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77316.1"
                     /translation="MKRIYIFLLIILFAAMLFAENRSLMVLKSLAVPGLSQINSGKEY
                     GYVMLTTEALIIGSLFYFNSESDILKQESYEYAIKYAHLAPGHYSDTYFKHLARYESS
                     GFDAGGYNNWIRQTALELYPYDPAMQQQYIAENCYTDDQYWYWDSSENRNQYNKIRNR
                     SNDYRDYAMVTGGILLLNHLTSAIDVMRWSAEAKRGNISFSLKERTPILQLNYQW"
     gene            complement(19808..20467)
                     /locus_tag="PLB65_01945"
     CDS             complement(19808..20467)
                     /locus_tag="PLB65_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77317.1"
                     /translation="MDVLKYKFWLIVLIVLSASIPLLALPKIVYPVSSLILPGSGELL
                     LGHNTRGATLLGADLVNLYAFYATDKEIDLQKENYMKFAELYAGVPYGMPKQHYQAVQ
                     NYPSSSYYNNIQDMMARNYYLIYNYDPQSYTDYIAANTYQGDEEWNWQSEDKWNQYKA
                     IRKRHQKAKMNNQLCLGLLLLNRCLSVIDSSILSKKEMGNVYLSPLSNEGAMLHYQID
                     F"
     gene            complement(20455..21423)
                     /locus_tag="PLB65_01950"
     CDS             complement(20455..21423)
                     /locus_tag="PLB65_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77318.1"
                     /translation="MKKTALLSLLLIFSFCLFGQNKQETDIDQWLYQTEEENIDASRD
                     TVITNDLIKVNYNQKSAHLAMAMSMLIPGTGQFYANRTAITTYIFPILELGFIGGIVY
                     YNKQGNDKTDKYEKYANGELVTYLTPDGTPISGYRYERGRQNQIQDILKDINTVDIYE
                     DTYFRLDENNSQHFYEDIGKYPHYVFGWMDWYYRFAADESGNFVTPRWQFDGPNDSPH
                     THWTGNYPICGQNTDIAISINSHEMSRMRQTYIKMRNDAKDDYGIAHLFTFGLAFNHL
                     VSGLDAVRVTRNFNRYYISQAAPSFNFYAAMPSGNVTPMVGFKWMF"
     gene            complement(21442..24576)
                     /locus_tag="PLB65_01955"
     CDS             complement(21442..24576)
                     /locus_tag="PLB65_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77319.1"
                     /translation="MKKLSLLLLSLLVISILPAQKMFLSKTGEYSKLLKHPYLDQLIQ
                     SNSKLDAIRKDSANYNRLLVILVDFTLEEPDDFNTTGNGKFQLEPDPEYIYSIGAPPH
                     NRQYYEANLDALKYYYLAVSAGSYELQYDVYPKDKVAYTLPKSMGYYNPPGASSELFV
                     SRMEEYFKTAFELADSLDEEIDFASYSHYMIIHSGSDWQHDISANTPSDLPSFYIRVG
                     DGKEAVVDNGTVLISHSCNVPETISQDFYISQSNGQNIHNGYGALNSVIAHEFGHSLG
                     MVDLYNVYNSSPMVGVFDIMDSGGSGILVDELENGDIVYVEGALPTLPGAFSRNLMFR
                     DNYLERGLLKEFPDFPVSSELSLNAISAMQNTTDPVPHTYKIPLNAKEYILVENRNVD
                     PDGDGGTAVYGALDGRVVLYPTPLEDITPPPPTYEYDYLLPSFIKTNGSAIGGGILVW
                     HINEAILYDEGQTDSFGNWTSNFDANTVNTNFYRKAVSIIEADGLTDLGSEYSMYWTG
                     TPYDYFHTHKPILDSGGLFVNWSQELWKPELTPNTKPPLVDSFNMPGFYHLKQISNPS
                     RKMSFRVESGLFDSTQILDYDSATTITGPVINSGFSEWNIPVLKTGNVNLLSNNNGIW
                     QDLMGDYTIPFSSTDYPLQRMDINQDGFWELVCIENNQLYSIDWANDDLIVKTITFPN
                     PPTTTPLSMDNSLIVGTDEGIYALNNFVEPYYVSIRGIKCLAGYDDKIIALGENYFKI
                     FSTVDLSLISEFSLPESFGNIEPLIYTNPDRTITMIFVMSNSGNLYRLYQNRLELIFQ
                     NHSNNTPGQLACTSLGDISPVLFFGIGNQFYALKADGTKLTNFPIVSYDTVSSWESPM
                     ALQLNVDETLFYPLRGSGYLAVDKYGKIVPDMSLSVNREHKSDYLFYNQQQQSLCWYY
                     TDNSGKLYIHTKNGISTNPILFAGFRNGASGCAIFDFKDESTSSTEKYAYIYPNPVRK
                     NYFKINLQNYYGQTKLWLYDISGTLIRNLVIPETLNNPRDFELSTKGLSSGVYIMILE
                     NGCTTKQLKFAIEK"
     gene            complement(24775..25800)
                     /locus_tag="PLB65_01960"
     CDS             complement(24775..25800)
                     /locus_tag="PLB65_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOQ77320.1"
                     /translation="MLKHHILVFSLMLMLVLPFTLSGISQAAMLFLTFEPGARANGMG
                     RAYSAIADDAYSMWWNPGATAFNKDTQIAATHIPWLQGSGIEDIFYEYLGFNHYFYGI
                     GNINAHIIWLDAGTQTQTSSSNVDLGDFHTYEVSGALGYGYEVVPDKIGVGGNFKLLY
                     SYLGPSAGFTESEGSAFSFAFDAGALFKDVVVDNLNASIVLQNIGPDITYSDEEQADP
                     CPMTFRAGLGYTVFDNPMGRLITTAEMSKILANEDPLYQRLITGWKYFDETIYGVGAE
                     YTYLNLISLRGGYYSDKEGEVEGASFGVGVEYKFSKRYKLGFDFAMVPAGGLTDYNKI
                     FSLGFEF"
     gene            complement(25989..26075)
                     /locus_tag="PLB65_01965"
     tRNA            complement(25989..26075)
                     /locus_tag="PLB65_01965"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26039..26041),aa:Leu,seq:caa)
     assembly_gap    26269..26368
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            27442..27891
                     /locus_tag="PLB65_01970"
     CDS             27442..27891
                     /locus_tag="PLB65_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ77321.1"
                     /translation="MLIFKVLHSRKELPENLTQKDIVDFLYTHLERFRDTSFAISKAI
                     DYAFSNEPGKGGFVILANTGDKLCGVLVMIKTGMEEFIPENILVYLAVHSAIRNRGIG
                     SELLKKALEIVKGDIALHVEYDNPARHLYERFGFTSKYAEMRWQKQY"
     gene            complement(28117..30255)
                     /locus_tag="PLB65_01975"
     CDS             complement(28117..30255)
                     /locus_tag="PLB65_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOQ77322.1"
                     /translation="MKYSDYETAKRTRIKWLVVMAIFTLATINLFSYNKGDYRTKWGG
                     NFEDLSLWEYYNGSGWENATQLPSSPFANTIYINTQNIAIHTSIIIEGGLVITGTLQL
                     YSGIAVTINKDANCEIGTIQIYSASKLINNGKIKNGNTNSSITVMDGGTLENNGLISN
                     SYESQNCQVTANSNGIIIFGSQGSITGNCSFTANYGSTIATSNPQGMDGSLTCSGTII
                     YNRPYLIFNGSEPQITGSKIPEQVLGIDFNNPTGISLSKNIKLVYTSLVHSGTTLYFG
                     MSVIEEAWWGSGTFSMEDSSSIATANPNGFSSTGKTGSLQVGTRNYNSNGNYIFNGTV
                     HQQTGDFNPAPNPYTVNDIIFDNLAGVTLTNPITVVSTMELLNGDLSYNILPQGVDGY
                     YSPDVQKVSIDKNGTLMYNFMAESLPFQNNNAFVNRKWILKGNFNGTKKITFNWNEDE
                     DNYFNWNVHNFPKVYSSNSDEPLVTIWNPAQPRKISFIALSFLNAKDDVFYYIGRGMD
                     DTLPVTLSSFIATQTSSSTVKLSWTTQSETNVMGFNILRSNQSNLDTATSLGYFTPAT
                     NTSQIRTYQCTDQVFATGIYYYWLEVVDFNGTNSFYGPAIADIQNIGNQTPSIPVKEG
                     FHSIAPNPIDYEARIFYGIPSKSPTLIQVYNLKGQKVRTILNETKDSGNFHLLWDAKD
                     DQGSQLANGNYILRMDASGKVWTKKITILH"
     gene            30486..32087
                     /gene="ispH"
                     /locus_tag="PLB65_01980"
     CDS             30486..32087
                     /gene="ispH"
                     /locus_tag="PLB65_01980"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOQ77323.1"
                     /translation="MRRIIVAIDGPAASGKSTTAKFLAEKLGYIYIDTGAMYRACALQ
                     AKLEGFAIDDLSSIIPMLERIDIKISYSESGNIIWLNGRDVSQQIRTPEISAAASKIS
                     AIPSVRYKMVELQRKLGKDGGVVLEGRDIGTFVFPEAEAKFFMVADLEVRARRRYQEL
                     KAKGIESRYEDVLQELKARDEADASRSLAPLKPASDAILVDTSNLSIEEQVNKLYEIT
                     LRKIAELGESNSPNNSVPDGIEVYLAHSSGYCFGVKRAIQMAREAKVCDKPVYTLGEI
                     IHNPGIVQELEEKGIHVANNVSDLKDSTVIIRSHGITREEYETLQKNNNEIIDATCPY
                     VKRTHTILQKMTAEGYPVVIFGDKHHPEVIGLRSFGNDKTIVVAEDEPPPKINENSLV
                     LIAQTTQKIEKLNELVCKLLPNVFELRVFNTICLTTTLRQKATIEMAKKSDVVIVIGG
                     YKSSNTSALAKLSSQYCPTFHIENEMQLAGVDLSKYKRIGLTAGASTPEEMIIKVYNV
                     ILQKSGHFSTVKSIEEIPLFKEESC"
     gene            32081..34504
                     /locus_tag="PLB65_01985"
     CDS             32081..34504
                     /locus_tag="PLB65_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOQ77324.1"
                     /translation="MLTHDHNTVETEVKETEEKNPILETVLNESEDKMSIPTVEDEKE
                     EPSIAIPEETISEEPETDASEQVQSEEIAETLKLDTKRKELTQEEKEHQEMLSMYEES
                     FSSFKKGEIIEGTVVDISDKEVRVDIGFKSEGVIPISEFAYSGIPDTNSVIKVYISEI
                     ENGEGKVLLSKKKADYLINIESIKRAFTEKTSLTGIIRRRVKGGMIVDVHGIEGFLPG
                     SQMSLKPIPNLDQFIGKELVFKVVNIDEDHHNIILSRRQVLEEEAEIKRQELLKKIEI
                     DSELDGEVKNITQYGAFIDLGGIDGLLHLTDMSWGKINHPSEMLQIGDKVKVKVINFD
                     AETNRISLGLKQLVPHPWENIEIKYPEGSRVTGKVVNVKSFGAFVELEPGVEGLVHIS
                     EMSWTKKITDARKFVKVGDTINAIVLDLDKENKRISLGMKQMDPNPWLTIEDRYPIGT
                     VLNRKIKSLTAFGAFVEIEEGIDGLIHISDMSWTKRIYHPREVYKRNQEVEAVVLSID
                     RAQHRIALGVKQMVPDPWETLDQILPVNTEVKGKVSKLIPKGLLVDIPIGNDVVEGFI
                     PISHLAIPKLEHSEDAFYVGEELPLKVIELDMENRRLILSVKAFFFSRDPKLQDEYIV
                     LHEQYMRDKVARQLKKKQEKEMREKKLAAKKDQEEAEKAEETVEKSPEPVAEEVSVAI
                     EPETEKTEIQQEPTTEEAPIETEKAEIQQEPTTEEAPIETEKAEEIPVEIEKAEEAIE
                     AVQKEEVIFEPEEKNVSEEQINEETITEETAIDSSVSEEQKVPEKADDASSKPEETSA
                     PEEESDKEK"
     gene            34556..37960
                     /locus_tag="PLB65_01990"
     CDS             34556..37960
                     /locus_tag="PLB65_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ77325.1"
                     /translation="MKRITVLLTVLLLLSINLKAELVTAGNGKNEVTLLEDNETETIL
                     QYRIAEFEKTKISLEGEDWYHIKLPKEGITQDKGFPELPVLNRSIIIPDQALMAIRVF
                     EVEFTDYQLRILPSKGVITRDINPDEVPYPFNDIYKNNCFYPKDIATLSEPYILRDFR
                     GITILANPFAYNSVSGILRVYTSFKVRVYVQGTDSINIISRSRASISRSFYHIYENQF
                     LNWSRERYISVDDNFGKILVICNANYLNQIAPYVAWKRQKGIDTELIEFGSIGTTADQ
                     LKTYIQSRYNIDNDITYVQLVGDVPYIPTLFYAGGGSDPSFSLVAGSDNYPDIFIGRF
                     SAETAAELTAQVNKSIQYERDLNTTATWLSEAIGIASAEGGGSLGDNGESDITHMNLI
                     RTDLLNYGYSSVDQIYDPGAPASIVSSSVNSGRGFINYVGHGSNTSWSTTGFNNSNVL
                     ALTNGNKTPFIMDVACVNGNFVSITCFAEAWMRNENGGAVGIYASSINQSWNSPMCAQ
                     DEVTDLLTSEAHTTLGGLYYNGSCKMMDAYRSDGVNMFRTWNIFGDASLQVRSKTPLA
                     MFVSYPMSITSGTNSITVSTGVSNALVSITNFETIYGIATTNSNGIAVVDLDNSPVGE
                     FTYTITVTAFNRVTYIGSLQQIYNPIPIQPRFVAEWEPAQGAIIRYPIGLPYSFIADI
                     SNNGLLYIIVSNSLQQSCNSVLTSNGVNMANVRYINTSSDSYWIRDYGPWTIMDSSNN
                     LQLVDFTYNRPRPNDDIVPSAIANYLGVNLYQMNLTHTGGNIITDGQGKAMSTNLVLT
                     ENPSLSQSSINQMMNSYLGITEYQLFEDPTNTYIDHIDCWAKLLDVDKVLIIRVPTNH
                     PQYNAIETIVTQWQSTLSSYGTPYRIYRIDTPNNEPYINSYILNKQIYVPLMGTANDS
                     AALLTYQYAMPGYTITGYTYSSFESTDALHCRVNTIFDKQMLSVKLTPPIPSPLLQEL
                     PVNIKIHHSYSLNLNATYVFWKIGQNGTWSSSNLIYQNNDNWICNLDAPLIGDTLFYW
                     ISATDISNRNVSAPLCGAQDPFMIVGMYVPHLNSPQAQMQIQGSSIRIYWNAVNNANN
                     YLIYACDTPDGTYNLVGSTSETFFITLASENKQFYKVVAQRTVNPR"
     gene            complement(37964..39022)
                     /gene="wecB"
                     /locus_tag="PLB65_01995"
     CDS             complement(37964..39022)
                     /gene="wecB"
                     /locus_tag="PLB65_01995"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOQ77326.1"
                     /translation="MMKIAQIVGARPQFIKLAPLSRKIRQRHQEIIIHSGQHYDLQMN
                     EVFFRDMEIPAPDYNLNVGSGSQATQTAAILTAIERILENEKPDLVIIYGDTNTTLAG
                     ALAAAKLNIKIAHIEACLRSFNKSMPEEVNRIVADHLSDILFAPTETAMQNAHKEGLL
                     NKTFQVGDIMVDSLIFGIKKAEKESKVLSALQLEDENYCLLTLHRPYNVDNPDNLLHI
                     LSGLNTLQRKIIFPVHPRTRNILKKIKDNHYHHIHYIEPQAYLDFLLLMKNADMIFTD
                     SGGIQKEAYILKKPCVTLRPETEWLETVQSGWNLLLPVASKDFPNEINKFTPPSEHPD
                     LFGKDVAELILQTLETFL"
     gene            39413..40036
                     /locus_tag="PLB65_02000"
     CDS             39413..40036
                     /locus_tag="PLB65_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77327.1"
                     /translation="MKKYLLIGLVMLCFFSMEAITKETEQKEINRDTGMGFQFGTLSG
                     NGFAYRNYWEKCGLQIVLGGMTTGSKDIDYELYGYYGGASEITKTENGRKTTISTSMN
                     YLHTLANNNIGRFYAFGGGSLIYSRIRQFEVDYRRSSNNYYVRILDHPERKKWDNKYA
                     YYFGFGLGFDFDIGQNFHWVLELPITIDEDSEIVMYIPQTGLYYFFK"
     gene            complement(40138..40554)
                     /locus_tag="PLB65_02005"
     CDS             complement(40138..40554)
                     /locus_tag="PLB65_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HOQ77328.1"
                     /translation="MKSYRKELHILTDKRRDYINITPEVRSVLHESGIKEGLCLVNSM
                     HITSSVFINDDEQGLHSDFEIWLEKLAPEKPHSQYHHNGFEDNADAHLKRQIMGREVV
                     VAVSNGELDFGHWEQIFYGEFDGMRDKRVLIKIIGK"
     gene            complement(40551..41255)
                     /locus_tag="PLB65_02010"
     CDS             complement(40551..41255)
                     /locus_tag="PLB65_02010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ77329.1"
                     /translation="MSDKPQIKAIIFDLDGTLIDSIVDIANAMNASLKELGYPEHPVE
                     AYKSFIGDGQMELARKVLPEDKRTPENIDALAKKYWDNYDIEWYLHTNIFSGVLYLIQ
                     LAVARKIKLAILSNKPHYFTKKMIRHFFRGAMIRHTKNPFGVYSGEEPDKPRKPDPTV
                     ALELAERMKVKPKYCAIVGDSVVDIKTAKNAGMIAIGASWGYGNKKDLQEAGADLIFD
                     SPTQMSAYLDSLPLCL"
     gene            complement(41293..42117)
                     /locus_tag="PLB65_02015"
     CDS             complement(41293..42117)
                     /locus_tag="PLB65_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HOQ77330.1"
                     /translation="MPVVVSVAKLITDFITPEKISLAIRVVLILVIGIPIVRLIRAIT
                     KKIVKDRLSPQSEQLVIRTVYYIAVLILLVTLLNEFGFHLSAILGAAGIFGVAIGFAS
                     QTSFSNIISGIFLISEKPFKVGDVVQVSSNIGTIESIDLLSIKLKTSDNRFIRVPNET
                     MIKTEVINITRYPIRRADVNITVSYSDDLQKVRDVLLDIVKNEPQALTDPAPLFLVDK
                     FADSGINILFGVWSKREDLFDLKTSLMIAIKERFKQENIEIPFPHILIANAEDIKC"
     gene            complement(42372..42971)
                     /locus_tag="PLB65_02020"
     CDS             complement(42372..42971)
                     /locus_tag="PLB65_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HOQ77331.1"
                     /translation="MKKGHIILLVVILLIIILAGWFIGRYNTIQKEKVNVETAWAQVQ
                     NVYQTRYDLIPNLVETVQGAANFEKSTLTQVTEARSRAGGNINLTSESLTDPQAFQAF
                     QQSQAGLSDALSRLMVVVERYPELKANQNFLTFQAQLEGIENRIRTERMRYNDSAKKF
                     NQLIVTFPNNIIASIIKVKPFQFFQAEDSAQKAPKVQFD"
     gene            43315..44523
                     /locus_tag="PLB65_02025"
     CDS             43315..44523
                     /locus_tag="PLB65_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77332.1"
                     /translation="MKQLSLMQKLVCMGLLIIPFGLNGLETKLSGEFWGRWLSEIAKR
                     KDSNGNYEDKLVNNYFSLERGYLGLETKFIDQVKGRFTVDVFTTDKTYEYQTYSLEDS
                     TLISSSKSGSIDGVGLKIKYAYVDFGGLIPIKDLSLTAGMQKVYFGSIYDWNYTLVSK
                     APSDEHKVVNSADLGITVNGYLPSGYGEYAFGIYNGEGYKKVGANLKDNTDFAYLGNL
                     RITPITGLTVGGSYMYNTVGRDKDLNGEVTNPSYEKQQLMDIYTCLAYGPVNLISECI
                     IKKVDYPNLSDSNNTKEYTARGISIFPILNLKDFVKYDLQLVGGYDIWDESDRIETDK
                     ARNKVIASTIGVNYNFMPDETYTPVMQLQLNYIDKNYDEDKSHSDYANGRRDSSQIIL
                     QLKWKFANIL"
     gene            44542..45360
                     /locus_tag="PLB65_02030"
     CDS             44542..45360
                     /locus_tag="PLB65_02030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ77333.1"
                     /translation="MKSIIKSVAPMLMMLLAFSLNAETKNQITCTGSTTVLPIAQATA
                     EVFMNNHAEINISVRGGGSGVGIASLQNKTTDICNSSRPLKSKEISTAKAKGINPIPY
                     AIANDAITIVVHNSNLLNNLSIKQIKDIYTGNIINWKELGGANVPIVVISRDVASGTY
                     EVFNDKVLAGAKVIGSAQLLASNNAVISAVSNTPGAIGYVGLGYLNDNAKLLSVNKVT
                     PSELTVKNNSYPLSRKLYMYTNGKAKGIVANYIGFIQSDRGQKIVVEQGFINIK"
     gene            45429..46301
                     /gene="pstC"
                     /locus_tag="PLB65_02035"
     CDS             45429..46301
                     /gene="pstC"
                     /locus_tag="PLB65_02035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HOQ77334.1"
                     /translation="MNKKYSEKAFQVLTYSTSLFTIIALLAIIYSLFKEGLPLFNKVP
                     IFDFLFGCNWYPTNVEPEFGAWTLIVGSLSVTIGALLIGIPLGLGSAIFISEVAGNCL
                     KEIVKPIIELLAGIPSVVYGLFGMSFLAPQIRNWLNLDTGLNIFSASVILGIMIVPIV
                     ASMSEDALSSVPKSLREAALALGSTKMECIFLVIIPAAKRGILGSIMLGVGRAIGETM
                     VVLMVAGGSAQIPQGVFSSVRPLTSTIAAEMGETVIGSLHYQSLYALAILLFLITFFS
                     NLITEFFILKRERK"
     gene            46298..47152
                     /gene="pstA"
                     /locus_tag="PLB65_02040"
     CDS             46298..47152
                     /gene="pstA"
                     /locus_tag="PLB65_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HOQ77335.1"
                     /translation="MIRRKIVNNIGLSLLTLLLCLTILVLLIFLVRIFYLGGKVLSWE
                     FLFTAPRNAMQEGGIFPALIGTFWLTVCSMMIVIPLGVLTAIFLTNYGKPKCLVNIVQ
                     TAINTLAGTPSIIYGLFGMAIFVNLLRFNVSLISGAFTLAILALPLFITSCTEAIKSV
                     PIDFYEASLALGATKKQTIMRIVLPTALPNILTGIIICIGRVAGETAPIMFTAATFYT
                     RRLPKSLSDEVMALPYHIYALMTEGTHPEYQKSIAYGTAVVLLIMVLGVSATAILIRS
                     YFRRKRKW"
     gene            47146..47904
                     /gene="pstB"
                     /locus_tag="PLB65_02045"
     CDS             47146..47904
                     /gene="pstB"
                     /locus_tag="PLB65_02045"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004810563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HOQ77336.1"
                     /translation="MVNEILTTENLDLWFGQNYVLKNVNLQVLDKRVTAIIGPSGCGK
                     STLLRCFNRMNDLIPEIRIKGKVKLFNEDLYAPKTDVTQIRFRVGMVFQKPNPFPKSI
                     YKNVAYGLQIQGKYTKNEINDRVETALKAAWLWEEVKDRLHSPALALSGGQQQRLCLA
                     RALANKPEVILLDEPTSALDPQSTAKIEELCLELKNNVCILIVTHNIAQAGRISDYTA
                     FMYLGELIEYGATEKVFTVPKDKRTESYLTGVFG"
     gene            47922..48587
                     /gene="phoU"
                     /locus_tag="PLB65_02050"
     CDS             47922..48587
                     /gene="phoU"
                     /locus_tag="PLB65_02050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HOQ77337.1"
                     /translation="MLSDRIQELKKMLMKEAGLVEKMVALSMDGLFYNNTGFLAEVNN
                     FENQVNQIEIELDDKCFTLIALYQPEAKDLRKILMIYRITNDLERLGDLAVNIAESAA
                     ELYSSPVLKLVPELFTMKEAALIMLRDAIIAFTAEDYILSRQVCDADNEVDNYNRYIY
                     KKIASLIRENPSQTKLYLHILRIAKNLERIGDLSTNIAEDTIFLVQGKVIKHNIETKE
                     ESI"
     gene            complement(48793..49314)
                     /locus_tag="PLB65_02055"
     CDS             complement(48793..49314)
                     /locus_tag="PLB65_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ77338.1"
                     /translation="MNYSQTDKLITQYSNFAYSVARIYRNKGLDWEDLKQESLIGLLK
                     ASQHYDPGKNVKFTTYASYWIKKQICEALKKDTRHLEEELHENIPTDNNIDQNPDYSN
                     YNLNVRKENTEYPQFSFNFPSDMPSIEINILTLSFQENKTLKEIAELMSISVERVKQH
                     KKKALRRLKKHLQ"
     gene            complement(49333..50643)
                     /locus_tag="PLB65_02060"
     CDS             complement(49333..50643)
                     /locus_tag="PLB65_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ77339.1"
                     /translation="MKKTSITSLLIFAFAFLIFLGFILILTRVPIVLILIITIIIAAL
                     FVWILHIFLQKQFSLILQTTAQIVRGNHTIRIPELNTKEFSTLQEDLNRMLEKLDKTI
                     HHLAVHREELRLVLSSIDDILWSQNLEGKLEWINQSFQQLFGEINPKQQIYYWEVIRD
                     PVLLSFIKNSENSKDKLLKEAEIEGHYYLFSSTHNDVAKRRVFILQNIDEIRQVEQMK
                     KDFIVNLAHELRTPLTAISGFAEAMEDSINVTNRRYLHIIQNHTKRLIHLISDLEQLI
                     RLERSGSIELQEINLITFLNNISLILSPIIEEKGLYLKLELDDNLQRLVCDPFKLEQV
                     FINLVQNSLRYTTKGGITIKSKLIQKEAFIEVSDTGKGIDNIHLPRIFERFYVADPSR
                     NKTKSGTGLGLAIVKHIIQLHKGRITVQSELGKGTTFSIYLPLE"
     gene            complement(50630..51346)
                     /locus_tag="PLB65_02065"
     CDS             complement(50630..51346)
                     /locus_tag="PLB65_02065"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ77340.1"
                     /translation="MDAQDKKIFIVEDERDILELIRMQLNSAGFTAKGFETALPMLNL
                     LKIEHPDLLILDLMLPDIDGMEVCRQLKNDKATQKIPILMLTARTDLEDKIKGLEYGA
                     DDYITKPFETRELIARIKAILRRSDWESDKNVLSITENFIIDFNRYEVWVQGKRLDLT
                     LTEFKILQLLTKRPGWVYNRSQILDYLWGNDKIVIERTIDVHIRNLREKLGNYAYLIK
                     NIRGVGYQFLSEIEDDIEKD"
     gene            51560..54022
                     /locus_tag="PLB65_02070"
     CDS             51560..54022
                     /locus_tag="PLB65_02070"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ77341.1"
                     /translation="MSKYLIVGGVAGGATTAARLRRLDEKAEIIMFERYSYISYANCG
                     LPYYIGGVIKERDRLFVQTPESFKARLNVDVRINQEVIAINRKEKTVTVKRADGSQYH
                     ENYDKLVLSPGAEPLIPPLPGISDSAIFTLRNVSDTDKIKEYIENHKAQHCVIIGAGF
                     IGLEMAENLHNLGMKVTLVEMAEQVMTPLDYEMAAIVHQHLKTKEVEFYLKDGVSSFS
                     RNESGLQVHLQSGRTINADMVLLSIGVRPDSKLAEAAGLELGINKGIRVNSYLQTSDP
                     DIYALGDAIAYPNPITKMESSTYLAGPANKQARIVADNIVKGNIRKYKGSIGTAIAKV
                     FDITVGSTGVSEKVLKDAGIDYISSIIHTSSHAGYYPDALPITIKIVFNPKDGKLLGA
                     QIVGFDGVDKRTDMLAHILKHNETIYEIQEIEHSYAPPFSSAKDAVNIAGMVADNIIN
                     GLVNIIHWDDLSSLDKNDTFIIDVRTPDETALGTIEGSINIPLDDIRSRLKEIPENKQ
                     IVVFCGTGLRSYFAARILMQNGFKNVYNLSGGYLTYEYATQKQSNEDIFADDYIGKDD
                     NIYKGKEEKMERDIKTITADACGLQCPGPIMRLKNEMDKLAEGDILEISASDPGFAKD
                     VPAWCKMTGNTLLEVKTEGKIIIARIQKGGGDKISKSLESGNNKTIIVFSDALDRALA
                     AFVIANGAASTGKQVTMFFTFWGLNVIKKPNPPKQKKDMMATMFSIMLPRSSQKLSLS
                     KINFAGMGPVLMRQRMKTKNVDSLESMIMQAKKSGVKMIACQMSMDIMGVEPSELIEG
                     VEVGGVATYLADAEQSNVNLFI"
     gene            complement(54192..55529)
                     /gene="gdhA"
                     /locus_tag="PLB65_02075"
     CDS             complement(54192..55529)
                     /gene="gdhA"
                     /locus_tag="PLB65_02075"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HOQ77342.1"
                     /translation="MTINDFMAKVAAKNPGEPEFLQAVKEVVETVWDVYVNNPRFVKA
                     NILERIVEPERVIIFRVPWMDDKGEVQVNRGYRVQFNSAIGPYKGGLRFHPSVNLSIL
                     KFLGFEQIFKNSLTTLPMGGGKGGSDFDARGKSDAEIMRFCQSFMTELFRYIGPNTDV
                     PAGDIGVGAREIGYLFGMYKKLQNEFTGVFTGKGLNWGGSLVRPEATGFGVVYFTEEM
                     LKTKGETIKGKRVAISGFGNVAWGAVQKVNHLGGKVVTISGPDGYILDEDGISGEKID
                     YMVELRASNNDRVKDYADKYPNAKFFPGKRPWEVKVDVAIPCATQNELNGDDARSLIN
                     NGVFCVTEAANMPCTPEAMEIFLNSKILYAPGKAANAGGVATSGLEMSQNSIRLSWSR
                     AEVDEKLHGIMQNIHKACVENGKEADGYVNYVKGANIAGFLKVANAMCDQGIV"
     gene            complement(55922..57142)
                     /locus_tag="PLB65_02080"
     CDS             complement(55922..57142)
                     /locus_tag="PLB65_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOQ77343.1"
                     /translation="MKLEGKVVIAQGGGPTAVINQSLVGVTLEARKFTQVTRVYGALY
                     GVQGIVNEEFVDLTQETTHNLEQIANTPSSALLSTRDKPDEKYCMEIFKVLKAHDVRY
                     FFYIGGNDSADTVRIVNEQATKADYEFRAIHIPKTIDNDLVMNDHTPGFGSAARFVAS
                     AFTGANLDNRALPGVYIGVVMGRHAGFLTASSAMAQKYPDDGPHLIYLPERPFHRDTF
                     LSDVKRVYDLYGRCVIAVSEGIVDDKGIPIVTKLSNNQEKDAHGNIQLSGTGALGDLL
                     SDLVRTKLKIKRVRSDTFGYLQRSFIGCVSDVDQHEAREVGERAAHYAIWYNMDGSIS
                     IQRTGFYSVNYQLENLSEIAGKTRIMPNNFINTEGNGITDDFRYYVRPLLGSGFPVAH
                     RLRAPKVAKLLLER"
     gene            57296..58921
                     /gene="dnaX"
                     /locus_tag="PLB65_02085"
     CDS             57296..58921
                     /gene="dnaX"
                     /locus_tag="PLB65_02085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HOQ77344.1"
                     /translation="MSYIVLARKYRPQSFKEVYAQDHITEILQSAISTNRIAHAYLFT
                     GPRGVGKTSLARILAKSLNCVNGPTITPCNSCSNCAEITAGTSPDVIEIDGASNTSVD
                     DIRELQHELLYPASGSKYKIYIIDEVHMLSKNAFNALLKTLEEPPDNVIFIFATTEPQ
                     KVLPTIISRCQRYDFKRIPIDSIVQRLQEISLMEGITIDTESLYLIARKADGGMRDAL
                     SLMDQAISYCMNNITIDKVRQIFSVIPNQIYHNFLTLIYEKNAQTLINELHQIFEQGI
                     DLQEFISGMQDFIRILLLRKLQVQIKDINQDEQVMFDELAKLFGQNKLMYILSYLIAC
                     KTDLKTSSNPYLILEALMIKLCKLDEMEDIAKIIEKLNYGSITQTQPISNISSAQSQP
                     VQSKDSTKESAVKIPDAISKEETTVVKLQFTQDNLEQLWQRILTRAKKISQMCAIALE
                     STTSKKAEGKKLCLTVEGNTFYNCLKTNLEALESSISELFEKPVKISLHLQETEPPTK
                     VNIQHTTLEDIKQENPEIDKFVKLTDSVLVKTL"
     gene            58918..60021
                     /locus_tag="PLB65_02090"
     CDS             58918..60021
                     /locus_tag="PLB65_02090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ77345.1"
                     /translation="MKRVFPLLTLLYSAFLIWLKRGVHKIILEDKKKVVPEVFQEISI
                     IIPARNERTNLPNLLNSLTHLDYPEGKYEIILINDHSNDGSTEFLATQNIVPNLKVID
                     FYTETPPLIGKKAALQQGIDESRYDILAFTDADCLVPVSWLKEINRSLDKDIDYLLSY
                     SVLKRTKDGTIFRLKNFERSVYYALAAAGLYYKVPFTSSACNMVYRKKVFYESGGFEG
                     IGHLRSGDDDLLLMKMMPKIRKAAYNPSSAMQVVSIDGTDRNKHHQTNIRRASKFRYH
                     PWWLKGLSAFVFIYFCLFYLALWQVMSKKSTKTIQLSLFLKTIAEFAFSQSHLRQISR
                     SKLGFLYPVQILIFPAQFIYYAVRGTVGKYTWK"
     gene            complement(60022..60270)
                     /locus_tag="PLB65_02095"
     CDS             complement(60022..60270)
                     /locus_tag="PLB65_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77346.1"
                     /translation="MVYKVILVKIDQRSSEATKVQDILTKYGCNIRVRLGLHEVSNEF
                     CANDGLVVLEVQGDRDILDKMIEELNALEYVQAKLVEM"
     gene            complement(60283..>60757)
                     /locus_tag="PLB65_02100"
     CDS             complement(60283..>60757)
                     /locus_tag="PLB65_02100"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424926.1"
                     /note="catalyzes a two-step reaction, first charging a
                     glutamine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOQ77347.1"
                     /translation="RLALGKEVRLKYAYYITCNEVIKDSQGNIIELLCTYDEKSRGGW
                     TEDGRKVKGTLHWVCAESAIPVELRLYDRLFTLADMSNMEEGKSYKDYLNPNSLIVKQ
                     NCLAEENLHKAQIGERYQFLRIGYFNTDYESKPDALIFNRIVELRDSWAKLSKKK"
BASE COUNT        19377 a        10936 c        10887 g        19457 t
ORIGIN      
        1 tctgtcgtcc cgctgggact tcttttaggc attcttttta tcgccgggtt aaaaaacccg
       61 tcgttagtat gtatcgttcc taaaagaact ttaaagaaga aatcctctaa atcctaaaat
      121 cctaacccaa gtttcgcatt tttttagcta aagtattgct aaataaggga aattattctt
      181 ttggggacac tataatctga atttttgagt ggctgtgttt ttatgaattt tgatatctaa
      241 tagggaatag atttttttcg cttcttcttc aggttgggct gtttgtctaa tctgcactct
      301 ataaccgtct gtagtattga atattgtatc agtacgcata tgggttttaa gaatttgttt
      361 gatgctttgc caactgatat gaacatcttt gagagaaggt ttataggtga tacttttcag
      421 aatagcatac gctaatacgg aatagaacag atgtgcctct atccgaccat ctttctgatg
      481 atgaatggat cttgttccca attctgtttt caaatttcgg aaagcggact caacttcatt
      541 caaattcata taaaatttcc agatttcttc tacactgaaa tcggtatagt tggtttcaat
      601 tacataacaa cccggaaatt cggacttaca ggatttcttg tgcaggtcag ttattatcag
      661 agattctact cttcgtggat cagaactgtt tgactggagt tgaatttcat agttagttgc
      721 cgtagaacca tatttttctt tcaatcgacc gatctttacc caaatcttgt ctgccttgtt
      781 aatattcccc ttttgaatgg aactgttaag atggtttatc tcttccagaa agttctgttc
      841 ccaactggtc gttttgcctc tttctttgat ggctctggct tcgctgtaaa ctaaaacctt
      901 agctttggca gcactgacaa ttttcttcaa atagagcttt ccctgatgat tatccttgtt
      961 ttcggtaaat cccgctaagg atatgaactc agaggcaaat tcttttaacg aattaggcct
     1021 ttctaccaca aaatacttat attcttctcg ctccagcaag tgaatattat ccttggtagc
     1081 tatttcacga tccattacta tggctggagg ggtaaaacga tctattgaat cttcgctatc
     1141 tttaagttcg gttaatactt ccagcagggt atgaggttca gattgattac cactgtagat
     1201 tttactaacc atcggaaacc catcctgatc cactactaat gctaattcta ataaaggaca
     1261 atcatctcgt ttttccttac tctttccccg cttacatagt ttactgttga gccattttca
     1321 ctttttttgt gcgaaacttg ggttagtaga aattctattc cattttagcg acatacaatt
     1381 aatggtatcg taataacttc caaggaaaag ctcacttata aatctggaca tattatgata
     1441 cagataacag cttattcaag attgagttat tcatatattc tgtctgtaat ttagaggcaa
     1501 agatccgctg tattttttct ggttcacact gttttttttc ttaatctcat aataaacata
     1561 aaatccataa aacgatgagg acattgttct ttcgtaataa aaaaaaccgt cagcgggggg
     1621 ttgctgacgg tttgtgagct tttggacttt gtttcacata aacaaggagg agtttatgat
     1681 gaagttttgt ataaaaatct aatcaagtgc ctttaaaaaa gaaggtacat cagttcttaa
     1741 aggagggact cttccagttt catttgttac ttgttttggt tcttcttttg tggcaggttt
     1801 atttatgttt agtcgagcaa agatatcttt aatttcttcg tcggattctt ttacaatttt
     1861 tacattttct tcggttgttt cctcaatatt tggtaatcct ggaaagtcaa tcaaatgtgt
     1921 tttgccttcc ttatctaaac ctgtagcaat gatagtaacc cgtattttac cagccatact
     1981 atcatccaaa atgatgccca taataatatt ggcagcttta ccagtttcac taactataac
     2041 attagagacc tcgtcgaatt cactcatcaa aatatcgtgt ccggcagtaa tgtttagaag
     2101 taaagattga catccctgca agctgatgtg agtaagaagg ggattatcaa tagctgctct
     2161 ggctgcattg atagctctat catcaccttc ggctaatccg cttcctatta gagcataacc
     2221 catattttgc attatagatt taacatcagc aaaatctaca ttgattaaac cggttacatt
     2281 aattatatcg ctaactgctt tagctgcttc atagagaaca ttatcggcct ggtgaaaggc
     2341 gtcctctaaa gctaaagtag agtatatttc acaaagctta gaattgggaa tcacaagcaa
     2401 tgtatccaca aattcacaga ggtgcttaat gccatacatt gcattttcag ctcttttttt
     2461 gccttcgaaa ggaaaaggag aagtaacaat tccaaaagtt aaaattccca tttcacgggc
     2521 aattttggct ataatagggg cagctccagt tcctgttcca cctcccatgc cggcagttaa
     2581 aaaaaccata tctgcgccgt ccagatgact tttgatatcg tcttttgatt cttctgcgct
     2641 tctggcacca atttcaggat tagcaccagt tcctaatccc cgagttagtt tttttcctaa
     2701 ttgcagtttc atattggctt tgcttttagc aagatcgcgg ctatctgtat tggcagcgat
     2761 aaattctacg ccatagagat tattctcaat catagtattg agggcgtttc caccagctcc
     2821 ccctacacca ataattttaa tgttggttcc aatctggctg ggtttttcgt caaattcaat
     2881 cattattccc ccttttaggt aaagtccctt agtatttttt taattttatc gacgatttta
     2941 gtgcttttta tgttaggtaa agcaaagccc ttggtaaccg gttcagtatt tttacctgaa
     3001 gcatagtata aaattcctat aacagttgca aaggcaggat cttccaaacg gctgatcatt
     3061 ccgtttaaac gagagaggtc tggagtagca gtttttacat ttaggttaaa agattctgaa
     3121 agaacaatat ccagattttt cattttagcg gttcctccgc aaagaataat tcctgaagtg
     3181 acgagctctg gtgtataata atccttagct tttgtgtaac ataaagatag catttcttcc
     3241 actctatgtt gaatcacatt gctgaccaga tatcttgttt ttcgactgga ggcacgtccg
     3301 ctaatacctt caacatcaat ttcttctgtt tggtccacac tattggctaa ggcatttcca
     3361 tattcaatct tgatatattc ggcattacct aaagtggttt taagaccaat agccaggtct
     3421 tcagtaatgt tttttcctgc catagggata actaatattt tttccaaggt gccgcgatta
     3481 aaaatggaca aatcacatgt tccgcctcca atatcaagaa ctaaagctcc caggcgacgc
     3541 tcatcttcac ttaaaattga ttcagctacg gcaatatgat ttaaaacaaa attttccgga
     3601 tctatatcat aacctgccag ttgaatacac ttatttagat ttcgcaatgg agtaagttca
     3661 gcaagaatag tatatactct agcaattaga tggaaaccat tcatatttac aggattatga
     3721 atatcatcct gattatcgat tataaagtta tgcggaatac catgcaaaat acgacaacgt
     3781 tccaaacctt tttgaatttt aacactattc ttagcatcgt ttataacctg ttctgtatgt
     3841 tcctgtgtaa tttcaccagg ttcattagga atttcggagg ggatagaaat gcgtccatca
     3901 ccgatttgag ttctaatatg atcaccggta atattgcaat agatattggt agcagctatt
     3961 ttagcatcat tctcagctgt ggagattgct tttgctacac aaccggaaag agcttgaata
     4021 tcttttacaa tgccacattc gataccttcg gatgggcttt ctccgatgcc taaaatctct
     4081 aagcgtcctt ctccaatttc tttagcaata atgcaacgaa cattggaaga accaatatct
     4141 aaagctgtga tgatggattg tttcatttat tacctgcctt aacaacaact tgatttttga
     4201 agcgcaagtc aactacgctg tttctattta tatttccgtt ttcctgaacg aatcgataac
     4261 gggaaatttg cgttgcaaaa tcttcctctg atggaattat ccgagttcca gttttagcat
     4321 cgattatgtt aatagtgttg tctattaaat aatattctga aatctgaggc aaaaagtctg
     4381 gtgcttcttt cactactctc tgatgaagct gtaaaacctt ttgtaatcca gagtatttta
     4441 attttattcc cggtttgatc tgattgttac ttaagtaagt actataaatt ggtatatctt
     4501 ctttataaac tggcgtataa atgattaaaa ctacaccttc ggaatctaca ggatatagat
     4561 ctccttctat tgttttaaga taaattgctg ccttgcgttc agtaatatca agtttaatag
     4621 tatcgggtaa acgcttatgt aatttaacat tcttaaccct cgatagagta tttaactttg
     4681 cctttatctc agaagaagga actgtaaata aattcattcc tatataggga ctggtaattt
     4741 tataaaataa agtatcggga ataactttat tgccgcttat ttgcatcttt tggaacttaa
     4801 aaaggtctat gttagtaagc ccaaaccaga tacccgatcc aataattgct acacctatta
     4861 gtgcaaagaa aaagaacaga taatatctgc tattaccgcg tcgcttacga gtacctttgt
     4921 ttagttccat aactttatgc ctcaaagtcc tttatctgtt taactgagat tttagtaaaa
     4981 tcaatctcag ctttacaagt tactactttt ttatatgtta tgctttgtgt caacataatt
     5041 cttttaactt tcctaaaatt tcttctccat acttccaaat gttacctgct cccattgtga
     5101 ttaggatatc tccctcttta agcaggggaa gagttcggct tactatttcc gaattagatt
     5161 caactaaatg aacttgatga tgaccacttt gaatggctgc atcagcaatt aatttggatg
     5221 ttatatctgg aataggttgt tcccgggcag gataaattgg ggccaaaatg aggcaatcac
     5281 aagaaaagaa tgatttaccg aattcttcgt aaaaatctct tgtccgagaa tataaatgag
     5341 gttgaaatag tactacaatc cttcttttaa cactatcttt aaaaccttcc agagtagctt
     5401 ttatttctgt tggatggtga gcataatcgt cataaactgt tattcctgcg gcttctcctt
     5461 tcatatcaaa ccgtctataa acaccgttga attcttgcag gcctaactga atagcagtaa
     5521 aagggatatc caattccaag ccaatagaag ctgccagcag agaattttgg atgttatact
     5581 tgccggtaac attcaaattt atgcgcccca gtttatactc cttataggat agatcatagg
     5641 aagatttgaa gccttgcatt tcgatatctc tggcttgtac atctgcttgt cttgaaaacc
     5701 cgtaagtaac tatcctttta ttaataaagg gcagaattga ttgtactcct ggatcatcca
     5761 aacaggcaat aacgcttccg aagaagggta ctttattagc atattcaatg aaagaacttt
     5821 taatgtgatc caaatcttga taacaatcaa gatgatctac atctacattg gttatacccg
     5881 caatacaagg agttaaagct aggaatgagt gatcatactc gtctgcttct acaactatat
     5941 actttccgct tcccatcaca ttggagctac caaagttttt tactttccct cccacaatga
     6001 tagttggttc taaaccagcg ctttctaaaa ctgaaccagc catagaagta gaagttgttt
     6061 ttccatgcgt tccagaaata ccgatggaaa agctcattct ggtgatttca gctaacattt
     6121 cagcgcggcg gataacagga attttctttt ctttagctgc caatatttca ggattatcat
     6181 ctgttacagc actggatttt actacaacat ctgcatctcc aaggttttct ggtctatgtc
     6241 cttcttcaac tttgattcct aatgcttcca agtgtgctgt aatttctgat ttttttaggt
     6301 ctgaaccagt aattaccaaa ccctgattat gtaaaaattc tgcaattccg ctcattccga
     6361 ttccgccgat tccgacgaaa tgtatttttt ttgttcttcc gagcattatt cccccttgtt
     6421 ttgcagatag ttaattatat aatgaacgat cttttcagct gcctgatttt tgggaagcga
     6481 gaacagatta tcttgataat atgccaggtt ttcgctcatc tttttaagac tctcaaccag
     6541 agttaagagt gataggtctt tctgttctaa aagcatagca actccctttt cttgctgagc
     6601 cagagcatta tagtattgat gattttctgc cgcggttgga agtggaataa gaatagcagg
     6661 aatacagttt tcttccagtt cggcgatagt catcgcgcct gcacgcgtta ttgctaattt
     6721 gcatttagaa agcatcttgg gaagttcggg cgaaaaggca aacatataaa caccttcggt
     6781 atttttatgt ttggttgcga aacgctcgta actagtttta cccgtttgcc agattatttg
     6841 atatcccata gataatataa ccggtatggc agaactgaca acagtattaa ttgcaattga
     6901 accttggctg cctccactaa ctataatagt aggtttattg ttatccaggc ccagagagct
     6961 tagttcaaaa gttttctggg tactgctgat tttgctttgt ctaagaggaa ttccatagtt
     7021 tacggtttta gctttaacta agtatttctt gctattttca aacgagatga agatagtatt
     7081 tatctttttt gccatcttcc gagttaccaa accagggtaa ctattgcttt catggaagaa
     7141 caaagggacc tttaataaag aagcagctat agctgtaggt ccggcaacga aaccacctgt
     7201 gcaaattact gcttgaggtt tttctttttt taagatgctg attgcagcaa tgatactgct
     7261 ggctaaaaag aaagggaaca ccaaattttc caatttaaag gagcgatata atttttgtac
     7321 ttttatatag cgaatgggat aaccggctga tttacaaaga gattcctcaa tactattgcg
     7381 attgccaata aatagaatat cgtggttata tttctgtagt tcatctgcca aagctaaagc
     7441 tggagtgatg tgtccacccg ttccacctgc accaaaaata aacttcatac atatctccgt
     7501 tgggcactaa tatttaacaa cattcccaag gcgatggaat caatcaatag agctgaacca
     7561 ccataactga taaatggtaa ggtatttcct gtaggaggta aaagagacat agctacacct
     7621 gtattaacta aaacattaca gaatatattc attgctaaac ccactcccag aaattctaaa
     7681 aatcgatttt cttgagaatg tgccatccaa aaacagcgga aaaagagaaa acaatgtatg
     7741 ccaaaaacaa taagagcacc aacaaatccc cattcttcac caattaccgt atatacataa
     7801 tctgttctgg cttcaggaag ataataatgt tttgctcttc ctctggccat acctgttcct
     7861 ataattgctc cactggaaag agctgttaaa ctttccttca cctgataatc attatcttca
     7921 attttgattt cacctgcggg ttttaagaac agacagaact ttttgtatgt tgttaagcgt
     7981 ccttttctat aatctgctcc attagctaaa acgaggcatc ctgcaattat gccaattatc
     8041 gctattgtga taagaactct ttttttagct ccagcataga taagcatacc atagagtgtt
     8101 aatccaccta taattaaagt acttaaatgc cgttccataa taattaacag gaaagtaacc
     8161 cctgtaacca ctattaaggc aaagaaatta cctaaaaaac taccagttgt agagcttacc
     8221 agttcttcat tctttttatc taagatatga gcaaaataga aaattagagc cagacgcgcc
     8281 aaaacgcttg gttggaaatt tataaaacct aagtttattt gacgagtagc tcctttaaca
     8341 gtatttcctt tcaccaaaac tataatcagt agaagaatgg ttaagtacac aatataggga
     8401 gaaagcactc taagtttttc caaatttaga gtatataaaa taacaatagc gaaaatggca
     8461 gagattatca taaagaaaag ctgtctatag aaatagcgca tagaactctg aacagatgaa
     8521 atatccaaca taaccactag cccgataacg cacaataagc aataagtaat taaaattact
     8581 ttatcgaagc taacgatata gctttggatt ttacttcttt tcattttcct tgataatcct
     8641 atttacaaca agcttgaaga attctcctcg ctcttcataa tttttaaatt tatcaaaact
     8701 ggcacaggca ggcgaaagaa cgattatatc acctggaagg gaatcattat atgctttagt
     8761 tacacaatcc tcaaaatcag atatgcattc aagagggatt attccgttcc aggtttgata
     8821 cattttctct gatgtatcac ctgtcagata tgcctttttt actatttttt gcatatcgga
     8881 agttaaagta ctatagcttt cacctttgtc ggagcccccc ataataattc tgatgggttt
     8941 atcaaaacta tgcaaggcac tgcgaaccga atcggtatta gtagctttgg aatcattata
     9001 gaaggaaatg ccatttacgc tacccacaaa ttccaaacga tgaggcagag gcttaaaacc
     9061 tgtaattgct tcagcaattt ttgctggttc tggatataat gcataaactg caagcatagc
     9121 agccattgca ttagcataat tatgaggtcc tctaatttcc agattggaaa gtggaacttt
     9181 tgtagtaggc cccaattgta tatagttatc tactggataa gcataagctg tttctatctg
     9241 atgtaaggaa aaatataact tgctagcttt tatattctta gtatatagca taatttcagg
     9301 agaatccata aatagcacag ctgtatctgt tgctgtttga ttcttgaaga tgttcacctt
     9361 actaagggca taatcctcga aggtattgta gcgattgata tggtcgggag taatatttaa
     9421 cagtacagct acattcggct tgaaagtatc tatcagatct aattgaaaac tgctgatttc
     9481 caaaacgata taatctaacc ctggtttttc gatggagtaa ccacaaaaag ggtctccaat
     9541 gttacctgca agaatagatt ttttccccaa gctctttagg atatgataaa ttaagctaac
     9601 ggttgtgctt ttcccgtttg agccagttac cgcaatgatt ttagaatcat tggcttttat
     9661 ttggtaaccc agttcgattt cactgataag ttcaatcttt ctttctcttg ctttttgcaa
     9721 aatagaagta ttaagaggta taccgggact tactatcaag cagttacaat caagcagtct
     9781 gtctgtatgg cctccaaatt cacagggaaa gtcctttttc aggttttctg cgtttgggat
     9841 tttcgtctca ggacgcaact cacttaaaaa aggaataaca cctaattctt tcaatttata
     9901 agcagcggca attccactgc gagccaaacc taagataccg tattttttgt ttttctcaat
     9961 catctcattt actctcttta acgaagtttt attgtagata aacccatagc tacaagcata
    10021 gctgccacaa tataaaaacg cataactatt ttggattcgt gaatacctct aagttcataa
    10081 tgatggtgta agggtgcaca aagaaaaact cggtgacctt gtccatattt atgttttgta
    10141 tatttgaacc aggaacgctg tataataacg ctgcaggtct ctactacaaa gattaggccg
    10201 atgataataa agaagatttc ctctttcaat aaaatagaga taacagctcc aattccacct
    10261 aaggttaaag agccagtatc gcccataaat acttgtgcag ggtaactatt gaaccataaa
    10321 aatccaatta aagttcccat tattgcactg ataaaaactg ttagttcacc tgcattagaa
    10381 agaaactcca gatttagata atcggcaaca atataattac ctttcagata actcatcact
    10441 cctaaaccca gaaaagaaaa agctaaagtt ccagcagcaa gaccatctaa tccatcagtt
    10501 aaatttacgg cgttggaggt tccagtgata aatacaacag caaaaggaat aaatatccaa
    10561 cctaatggga ttatcaaatt cttaaaaaag gggatctgta aagctgttat attatcggta
    10621 acggaactgc tgtaatatat tgccaaagta ataattaaac caacggaaat ttgacctaac
    10681 agcttgtatc ggggaatcaa tcctttttta gctttcacaa aattctttaa ataatcatcc
    10741 aaaaacccca aaatacctag ccagacagtt gccaaataca tcattaaaat agctggattg
    10801 gttagtacat tccataacaa agaagaaatg agcagtgaaa ataagataat gattcctccc
    10861 atagttggag ttccttgttt taagcgatgt ttttcaggga ggtcttcatc aattgtttct
    10921 acggcagacc ttttacgcag cattttaata aaagttgggc ccaccaaaag cgatattatc
    10981 aaagaagtaa taaaagctcc aatagaacgg aaggttacat agcgaaacac attaaaaacg
    11041 atgctatatt tagcaagagg gtaaagaaga tgataaaaca ttagttctct cctttaagga
    11101 ttgggattat gcgctccagt tgaattgaat gtgagccctt aactagaatt attgcattgt
    11161 tgggaaaggt tcgtaaattt ccagaagcaa ttaattcttc cacattatta taatgcagga
    11221 tagcattttc tctatcagga tgaaacaatc gcgaaaagga accaacagtg atcagttttt
    11281 cgtagcgcat ttcagaaagc atagcagcaa tcattttatg atacataaca gagctatctc
    11341 caagttccag catatcacct aaaacagcat aatgaggttg ttctgaagat agtgtttgcc
    11401 aatattctaa tgctttttgc atagaataag gattagcatt atagcaatcc agaatcagta
    11461 tccctgttcc tctttttacc acttgatttc ttaattccag atagatggga gaatttaaag
    11521 ctgattgtat atcttttttg ctgattccca atttcaagca taacacaata gcaaaggcag
    11581 catttaaagc aaagtaaggt gctccataag gaataatcca taaatcctca ttcaaataaa
    11641 aggactggct actatccaaa atctgatgtt gcgtaatcct gtaatcacaa ttttcattaa
    11701 agccaatact taatccctgc ttggcaaaat attgaaaacg agaatcatct ccaggatata
    11761 agcgtaaagc aagaggtcta ttaaatagag cgatcttttc ttgaaaaact ccatcttcat
    11821 ccttaaaata ctctaaatga gaaggaccga tattcaaaat aataccaatt tcaggatcga
    11881 caacatctgc caaagaagca atttcgccaa agtgattggt tccaagttca aaaagggcat
    11941 aacgatcttc aggttttatc ctcaaaatag ttttacagag gcctatcata ttgttctcat
    12001 tagccagggt tttcaaagtg ggagcggaac tttccaaaat ttgagccatc aattctttgg
    12061 aggtagtttt accagtactt ccggttagag caattttgtg aggagcaaat agagctaaat
    12121 atttacggca gatagaaccc attgcttcca aactattatt cacctgaaaa tattgaaatt
    12181 ctgcgatagg agattcctct gaccaagatt tttcaccaat agcgcaattt tctttagggg
    12241 caagtacttc ttttagatag ttatggccat cgaatttctc tccctgaatg gctacaaata
    12301 tgctttcagg ctttagaatg cgagaatctg tatatagata tttatagtat actggctctt
    12361 tataaccaga aggttcggga ttccaatttg aaataaatag tagtttcatt agggtctgat
    12421 caacaggtag aataaactct gcgtcgtctt taatttttgt atcccttttt gccagtgctt
    12481 ccaaagtcgt ttcgagatca tcaaaatgat agtgaacccc ctcaatttct tggtagttct
    12541 cactcccttt cccacaaata agaacaatat catctttttg tgcaaaccga ataatggatt
    12601 ctattgcctg ttttctatct ctgataatcc accagggtaa aaatatatcg cttgaggata
    12661 caatatccct gataattgcc tcagggtttt cgctgcgtgg attatcatca ctgataataa
    12721 caacatcact atgattgaga gctgctttca acattagggg tctttttcct ttatctctat
    12781 ctcctccggc accaaataaa cagagcattc ttttatgagg taagtcctct cctgcttcta
    12841 aaacattttc aatagcctga ggcgtatgag cataatcgat aaaaatgccg ataccataat
    12901 tgttttccac tttttccatt ctgcctcgga cgggaggaat atcttttatg gatttgtgaa
    12961 tttcttcagg tttgaacccc attgcatata aagtgagagc tgttaacccc aaattttgaa
    13021 tattaaaatt accaatcaag cggctgataa tataaatctc atctttatcg gattgcaacc
    13081 gaaagctgct gttatttata tcagtttgta catcggcaaa acgaaaatca gcatcttcac
    13141 ttccaagaga aagtacttta gcgtgttttt caacaagcat ttcctttatc cttataccaa
    13201 aaggatcgcc ggtattgacg atggaaacgc taccatcttt tgcgccttgt tcaaaaaagc
    13261 tcattttcgt ttgaccatag ttttctatag taagatgaaa atcaagatga tctcgaccca
    13321 gattactgaa caaacaataa tcgaatttaa ctccataaac tctatttaga gatagtgaat
    13381 gggaagaaac ctccataact acataattta ccctttcatc tatcatctga gcgaatattt
    13441 catttagttc aataatatcg ggagtggttc tctcagaagg gaaagttcta ccttcaatat
    13501 agtaacccaa agttccaatc caaccacaac gaaaccccaa atctctgata gcttcataga
    13561 tcattattga ggtagtagtt tttccattag tgcctgttat cccaatcaaa gtaaatggaa
    13621 ttttttctgg aagcaacaca attcgagcaa taatagctgc cgctttacga ctgtctttca
    13681 cttttagata agggaaagtg atgttatcaa attcctgtcc tacaattaat gctgcacctt
    13741 tattaagagc ggaaggaata aaattatggc catcataatt tgtgccttta atacagacaa
    13801 aaatatcacc tggttgcaat tttctggtat cggtttgtac ttttccctgc cagttttgca
    13861 gttgttccaa attagctgtt tctatcaata gagaattctg ctttaaggca tttattattt
    13921 tattaatcat aacgatgcct ccagatggca tttactgcct tccaatattc tactccctgg
    13981 aagaatagat tgagagcgga caattcctga accacgaata ttgattgcca aaccgtgagc
    14041 tgatgcttgt tttagagctt ctcttaaact aagtcctatt aaatttggca taattcctgc
    14101 ttcgcggaca ggggcttttt tatctgcact ggggcctatc tttaaggtta ttggataatg
    14161 tggatctact gtaactcctt cttttggata ttgatcaata actacagagg cagaatctgg
    14221 cccctcgact ttatattgaa atccataata gttcaaagtc ctttctgcct ggaaaagatg
    14281 cattccaata agtttaggca tagttaatga ggtttgcatc aaacgttcat tataaggcaa
    14341 aatctggcaa tcgggcataa atagaatgtt ctccactatt tttttgaaag tgggagcgct
    14401 acttgtgctg ccaaaatgaa atccaggagc cggttcatca taaaaaacca ctattaccat
    14461 ttggggatct tcaatgggga acattcccac aaatacagaa ctatatttac cttcactgta
    14521 tcctttggtt cctgtaatat ttttttgagc agttccagtt tttccgccaa cctggatata
    14581 atccattttt atatgtttag ctgttccatt atctaccaca ccttgaagat atgactttat
    14641 agtatcagta gctgccggag tagatacatt gcgtaaaacc gtgggttcaa attgttcaac
    14701 tatttttcct tcttcatcgc ggatggtatc cacaataata ggtttcatca atttccctcc
    14761 attagcaatg gcacaatagg cagtggcaag ttgtaaggca gttgtagata taccttggcc
    14821 aaaagaaact gaatgtaatg tatagccatc ccagttttgc agtttggcaa acattccact
    14881 ggattctcct gtcatattta aagcggtttt ttggccaaaa cccaaagcta taaatttctc
    14941 atataaccgc gtggggccaa ttcgttccgc tattttagca atacctacat tactggattt
    15001 aactattatt tcgcgaggag taagatttcc atattgatgt gtatccgaaa taactcttcg
    15061 tccggtttgg tatctaccgc attgaaatgt ctccgttggc tttaccagtt tatattcaat
    15121 agcgggcaac atagttaaag gcttcattgt agaaccaggt tcaaacataa aactaacagg
    15181 aatattggtt tttactctca ccaagttcgg atcttcaatt ctatcatctc tggatactcc
    15241 agccattgct ataattttac cggtattcgg gtccataaca atagcgccag cattttttgc
    15301 gctgtattgt tccattccct caaatagagc attttctaca atttcttgaa ttttagaatc
    15361 aattgtaaga taaacattgt aaccgttttg aggttttttg gaatgcagat taggataggg
    15421 gacaggtcga tgattggcat ccaatactat ttctttccag ccataatttc cagataagta
    15481 tttatcgtaa gtggattcta ttccgcaaac tccagagagt tgatagagag atttactatg
    15541 agttgcagga tcgtaaccat cagatacctc gcgcactgaa cccattactc ttgcagccaa
    15601 tatatctttg gagtatattc ttctcattga agcgaagcta tgaatcaaac cgatcatttc
    15661 tttagattta aaatcccgaa tcaatttgtc cagttctact tcgctaatct tgttggaaat
    15721 ttgaacagaa gtgtttttat tgccaatagt taggcgtttt aacacttcat ctttatttaa
    15781 agaagtatga gcagcaatca attctgcata tttcgcgaac gcatcttcta atgttaagtt
    15841 attatctttt gcccacattg ccacggaaga tctatctata tctatttgat agtagcttat
    15901 tgagctaact aaaagattgc cctgggaatc aagaattgaa cccctacgag gaattaaaat
    15961 ttctttttta ggtgtatatc gcattcttct tactccagct aaatggaagg gatctaaaat
    16021 ctgaatgcag aatagatacg ctacccaaaa aattgaagca agaccaaaaa taacaactaa
    16081 aaagtaaaag cgagatttca tctcaatcca acaaaacctg tatgttttta gcttcagctt
    16141 tagtagttaa tagatctata atacaatagg tcttcttatc ttgtttagct gaaggctcgt
    16201 gtacatatat aattttacct gcttgttgat caggaataaa attgctcaat tctactctta
    16261 ctaaagaagc aatatgtctt ccactacgta aatcatcaag ttctactaat aattcagtat
    16321 tgatgttttt ttccgcataa taggtctttt ccagctcaga aatttgtctg gtatattgca
    16381 cgattcggtt attattgtaa aagcttagaa aaaacacaac tcccaatagg gacacaagta
    16441 tcaggatttt gccacgcatt tttcctcctg tgttttctgc cggtttgatt tgcggtaggt
    16501 tttttcctct ttgttttcct ctttaacttt ttctgcagca cgcaatttag cgctgcgcga
    16561 acgaggattt gccaaaattt cttcttctga tgcggttatg ggtttggaag ttaaaataac
    16621 taactgttta tgatggttac agttacaagc taaggaacgg ggagggcata tacattcttg
    16681 ttctgctctc ttaaattctt gtttaacgat tcgatcttct aaagaatgat aactaagaac
    16741 cactatccta cctttcggat ttaataaatt tatagcatcc ttcaaagtag aagcaagaac
    16801 ttccaattcc ttatttacat aaatccttaa tgcttgaaat atacgcactt tagttttaag
    16861 cgaggcctta gttccttttc caacaacgct ttcaattatt ctggttaatt cgcctgttgt
    16921 attcacaggt tttgggctcc gttctatggc tttagcgatc cgaaaggcat ttagctcttc
    16981 tccataatct ctaaatatcc tagctaattc tttggcgtct aattcattaa ctgcatcata
    17041 agcactatat tcttggctgc tatccattcg catatccaaa ggagcatctt tctcaaaact
    17101 aaaaccccgg ttttcattat tgagctggtg agaagaaaca cccaaatcaa aaagtatgcc
    17161 atcaatacct ttcactttac gataggcaag ctcagtacga agctgttgga aattagcttt
    17221 tatcaattct acattttcat agttacttag ttttttactt gcttcttcaa ttgcttccat
    17281 atcctgatcg aaacaatata atcttatgga cggttcagat tttaacattg ctaagctgtg
    17341 tcctccacct ccggttgttg catcaacata tattttccca gctctcaaat ttaaatacga
    17401 gatgcacttt tctgccatta ctggagtgtg aaatatcata gctgataatc ctctgaagta
    17461 aattctttgc ggtgttgatt aagtttgctg gcacgaactt cattataaac cttgggattc
    17521 caaagtgaaa tataatgccc ctcgccttta ataataacac tatctgtaat atttacttca
    17581 ttcagcaacc tttccagaat acgaattctt cccggaccct ctaattcctg ttctgtcata
    17641 gcaaaatcga ttaattgggt gcgaagttta ttgctatgct cgtcaccaat tcttaaacgt
    17701 tccagggttt cattccagtt atccaaagga tagatagcta ttgtatcatt gggccccaga
    17761 gtaacaataa ctttcttttc cgattcctca gaaaacttct ttttgaatgc tgcgggaatt
    17821 attacccttt gcttgttcac tgaattttcg aaaataccaa gaaattcacc agacatactc
    17881 gaaatccttt ttttagagtt tccctttata aagctctttt tctttaaagt ccgtgctttt
    17941 ttgactcatt ttttagaacc gccttgagat attttgagtt tttatgactt tctatggttc
    18001 ttatatgaga tctcgttttt attgtcaagt gcaaaatgcg tttttttata aatagataga
    18061 gtattttttt caacatcagg gttcaaggtg gaaagtttat cattttatac tacttatagt
    18121 tatggctata tactcttaag ctcatttatc aagtttaggg gcttaactat ttggcaggca
    18181 ataagttgtt attttcttaa aatatttttt ctttaaagta agctgtaact atgcaatttt
    18241 ccgcattgtt acaagcttac agctatattt tgggcgggaa aatatttttt tggcgcttct
    18301 tcaaaataat ccgattttgc agatattctg ggtaagctta tctctatttt tagaatacta
    18361 tgcccatttt cataaaggaa attggacgat tggtttttaa taaacagcat aaaagtgata
    18421 ctgttattcc ctttgaattt tgtaggttct atgtatagca gcggattatt gtaaatggat
    18481 cttactatat tcgatagcat tatggaaaat aatggcaggt tttgagccca gattcataac
    18541 actaagaatg caaagctgta tctattaaca ggccgaggga aaatcaatct tgaggttgca
    18601 aaacttcaaa tttgggctat aatatatata actaatattg cttaggagat aagatgatta
    18661 aactttcaga attgcctgta agttatggaa aagttcattt accagccaaa gtagcactgg
    18721 tagtaacgca aaagccgacg agtggttata atctaataac gattgagtgg tttatgcgca
    18781 cttctcttca gcctccgatg tttgcaatat caattggcga aagccgatat tctcatgaat
    18841 gtcttcaggc aaatcgatat tttaatttgg tctttccatc gcttaaaatg aagcagcttt
    18901 tatctttgtg tggctcgaat tcagggagag atatggataa atttacggtt gccaaagtgg
    18961 agtatattca gggtaaattg aataaattac cagttttgaa agatgccgtt gcatgttttg
    19021 aatgtgaggt tgtatctgaa attcgcagcg gagatcatac tatctttatt ggtcaagttc
    19081 attatagttg gcaaaatgag gaagaggaat tattctatta tcaggagaaa ctctgataca
    19141 gaaaaaggtt taccattgat agtttagctg aaggataggt gttctttcct taagagaaaa
    19201 agagatatta cctcgcttag cttctgcact ccaacgcata acatcaatag cacttgtcag
    19261 atgatttagt agtaaaatgc ctcctgttac catagcataa tctctgtaat cgttactgcg
    19321 attgcgtatt ttattatact gattacgatt ttccgaacta tcccaatacc agtattgatc
    19381 atctgtataa caattttctg caatatactg ttgttgcata gcggggtcgt aaggatataa
    19441 ttccagagca gtttggcgaa tccagttatt atagcctccg gcatcaaaac cgctactttc
    19501 ataacgggct aagtgcttaa aatatgtatc actgtaatgt ccgggagcaa gatgagcata
    19561 ttttatggca tactcataag attcctgctt taatatgtcg ctttcactat tgaaataaaa
    19621 aagtgagcca ataattaagg cctctgtagt aagcattaca tagccgtatt cctttccgga
    19681 atttatttga gacaagccag gaaccgccaa agattttaaa accatcaaac tacggttttc
    19741 tgcaaataac attgcagcaa aaagaatgat cagcagaaaa atatatatac gtttcaaaat
    19801 gttactccta aaagtctatt tgataatgca gcatagcgcc ttcgtttgat aaaggagata
    19861 gatatacatt acccatttcc tttttagata gaatggagct atcaataaca ctgaggcagc
    19921 gattaagaag taataatcct agacagagct gattgttcat tttcgctttc tgatgtcttt
    19981 tgcggatagc tttgtattga ttccatttat cttcgctttg ccaattccat tcttcatcac
    20041 cttgataagt attagcagcg atataatcag tgtaagattg aggatcataa ttgtatatca
    20101 gataatagtt tcttgccatc atatcttgga tattattgta ataagaacta ctgggataat
    20161 tttgaactgc ctgatagtgc tgttttggca ttccataagg aactcctgca taaagttccg
    20221 caaatttcat atagttctct ttttgcaaat caatttcttt gtctgtagcg taaaatgcat
    20281 atagatttac aagatccgct cctaacaaag ttgctcctct tgtgttatgt cccaaaagta
    20341 attctccgct tcccggtaat attaaagaag aaacaggata tactattttg ggtaaagcca
    20401 atagcggaat agaagctgat aatacgatta aaacaatcag ccagaattta tattttaaaa
    20461 catccactta aaacctacca taggggttac atttcctgaa ggcattgctg catagaaatt
    20521 aaaggaagga gctgcctgag aaatataata acggttaaaa tttctggtta cgcggacagc
    20581 atcaagccct gatacaaggt ggttaaaagc taaaccaaaa gtaaaaagat gggcgatacc
    20641 ataatcatct ttagcatcat tgcgcatttt aatataagtt tgcctcattc ggctcatctc
    20701 atgtgaatta atagaaatgg ctatatctgt attttgtcca caaatgggat aattgcctgt
    20761 ccagtgagtg tgagggctat cgttggggcc atcaaattgc caacgtggag taacaaaatt
    20821 tcctgattcg tcagctgcaa aacggtaata ccaatccatc catccgaaaa cataatgtgg
    20881 atattttccg atgtcttcat agaaatgctg agaattattc tcatccaggc ggaaataggt
    20941 atcttcatat atatcaacgg tattaatatc tttcaagata tcctgaatct gattttgcct
    21001 tcccctttca taacgatatc ctgatattgg agttccatca ggagtaaggt aagtaacaag
    21061 ttctccattg gcatattttt catatttatc ggttttatcg ttaccctgtt tattgtaata
    21121 tacaatccca ccaataaaac caagttctaa tatcgggaaa atataagttg taatagctgt
    21181 tcgattggca taaaattgac ctgtacctgg aatcaacata gacattgcca ttgctaaatg
    21241 agcgcttttt tgattgtagt taacttttat taaatcgttt gtaataactg tatctcggga
    21301 tgcatctata ttttcttctt ctgtttgata cagccactgg tcaatgtctg tctcctgttt
    21361 attttgccca aatagacaaa agctaaagat aaggagaagc gatagtaaag ctgttttttt
    21421 cattgtgaac tccttaatag atcatttttc tatggcaaac ttaagttgct ttgttgtaca
    21481 accgttttct aaaatcataa tatatactcc ggaactaaga cccttagtgc tcagctcaaa
    21541 atctctgggg ttgttcagag tttcaggaat tactaaattg cgaattaaag ttccgctgat
    21601 gtcataaagc cacagttttg tttggccata atagttctga agatttatct tgaaatagtt
    21661 tttgcgaaca ggattgggat atatataggc atacttttca gtggaactag tgctctcatc
    21721 cttaaaatca aaaatggcac agccgctagc cccatttctg aaaccagcaa aaagaatagg
    21781 attggttgat ataccatttt ttgtgtgaat atatagctta ccggaattat ctgtataata
    21841 ccaacataat gattgttgct gttgattgta aaaaagataa tctgatttgt gttcccgatt
    21901 taccgacaaa ctcatatctg gaactatttt cccgtattta tctacagcta aataaccact
    21961 acctcgtaaa ggatagaata gggtctcatc cacattaagt tgtaaagcca tagggctttc
    22021 ccaactggaa actgtgtcat aagatacaat agggaagttg gttagtttgg ttccatcagc
    22081 ttttagagca taaaattggt taccgatacc aaagaataaa actggcgata tatcacctaa
    22141 agaagtacaa gctaattggc caggtgtatt attactgtga ttttgaaaga ttaattccag
    22201 tcggttttga tatagacgat atagattccc actattagac ataacaaaaa tcatagtgat
    22261 agttctatcg ggattggtat aaattagtgg ttcaatattg ccaaaggatt ctggaagaga
    22321 aaactcactt attaagctta agtctactgt ggaaaaaatc ttaaaatagt tttcgcctaa
    22381 agctattatt ttatcatcat accctgccag gcattttatt cctcgaatag aaacataata
    22441 cggttcaacg aagttattca aagcatatat gccttcatcg gtacctacta taagcgaatt
    22501 atccatagat aaaggagtag tagtaggagg attagggaaa gtgattgttt ttacaataag
    22561 atcatcatta gcccaatcga tagaataaag ttgattattc tctatgcata ctaattccca
    22621 aaagccgtcc tgattgatat ccattctttg taagggataa tcagtagaag aaaatgggat
    22681 agtataatct cccataaggt cttgccagat accgttgtta ttgcttagca aatttacatt
    22741 tcctgttttt aacacgggaa tgttccattc gctaaatccg gaatttatta caggacctgt
    22801 tatcgtggta gcagaatcat aatccagaat ttgagtgcta tcaaataaac cactttctac
    22861 ccgaaaactc atcttacgag atggattgct tatctgtttt aaatgataaa aaccaggcat
    22921 attaaaacta tctaccaaag gtggttttgt gttgggagta agttctggtt tccaaagttc
    22981 ctggctccag ttgacaaata aaccaccaga atcaagtatt ggtttatgtg tatggaagta
    23041 atcgtaaggt gtgcctgtcc aatacataga atactcacta cctaggtctg taagtccatc
    23101 agcttcaata attgaaaccg cttttcggta aaaattagta ttcactgtat tggcatcaaa
    23161 attactggtc caattaccaa agctatcagt ttggccttca tcatatagaa tagcttcgtt
    23221 tatatgccaa accaagattc cgccacctat tgctgatccg ttagttttta taaatgaagg
    23281 tagaagataa tcatattcat aagtgggagg tggaggagtt atatcttcaa ggggagttgg
    23341 gtataaaaca actcttccat ccaatgctcc gtaaactgct gtacctccat caccgtcagg
    23401 atctacattg cggttttcta ctaaaatata ttctttggca tttagtggaa ttttgtaagt
    23461 gtgaggaaca ggatcagttg tattttgcat agcactgatt gcattcaagg atagttctga
    23521 tgaaacagga aaatcgggaa attcttttaa taacccccgc tcgagataat tatcccgaaa
    23581 cattaaattg cgagaaaaag cccccggtaa tgttggtaag gcaccttcca cataaacaat
    23641 gtccccattt tctaattcat caactaatat cccggaaccc ccactatcca taatatcaaa
    23701 cacgcctacc ataggagaag aattgtatac attgtataaa tctaccattc ctaaggagtg
    23761 cccaaattcg tgtgctataa cagaatttaa agctccatag ccattatgaa tattttgtcc
    23821 attactctgg ctgatataaa aatcctgaga gatagtttcg ggaacattac aagaatgtga
    23881 tataaggact gtaccattat caacaactgc ttctttgcca tcgccaactc tgatataaaa
    23941 agaaggaaga tcggaaggag tgtttgcaga aatatcatgt tgccaatcag atccagaatg
    24001 aataatcata taatggctat aactggcaaa atcaatttcc tcatctaaac tatctgctaa
    24061 ttcaaaagca gtcttgaaat attcttccat tctgctaaca aatagttctg aacttgctcc
    24121 cggaggatta taatatccca tacttttagg taatgtataa gcaactttat ctttaggata
    24181 tacatcatat tggagttcat aagaacctgc tgaaacagct aaataataat attttagggc
    24241 atccagattg gcttcataat attgtctgtt atgaggagga gctccaattg aataaatata
    24301 ttcaggatcg ggttcaagct ggaatttgcc gttgccagtg gtattgaaat catccggttc
    24361 ttctaaagta aaatcaacca aaattaccaa caaacggtta taattggcag aatcctttct
    24421 tattgcatct agtttggaat tggattgaat taattgatct aaataaggat gttttagtag
    24481 tttactatat tcgccagttt tacttaaaaa catcttttgg gcaggtaata tagatattac
    24541 caatagggat agtagcagta atgatagctt tttcatatta ttaattttga tcctgtgttc
    24601 gaggttactt tatatataac caatactact ctaatattga attatcaaaa aaattgtcaa
    24661 taacattata tcaacagata aaggtgttcg acgaggactt ccattcttaa gagacttagc
    24721 agccctcatc aaacaactac ctatatattt ttttttgcat tattttatta ggagttaaaa
    24781 ttcaaaccct aaagagaata ttttattgta atcagtcagt ccacctgcag gaaccatagc
    24841 aaaatcgaaa ccaagcttat aacgcttact gaatttatat tcaacaccta ctccaaaact
    24901 ggctccttca acttctccct ctttatcaga ataataacca cctcgtaaag agatcagatt
    24961 tagataagta tactctgcac ctactccata tattgtctcg tcaaaatact tccatccggt
    25021 tatcaatctt tgatacaaag ggtcctcatt tgccagaatt ttactcatct ctgcagttgt
    25081 aattaaacgc cccataggat tatcaaaaac agtataacca agaccggctc taaaagtcat
    25141 cgggcaagga tctgcttgtt cttcatcact gtatgtgata tccgggccta tattttgtaa
    25201 aacgatggaa gcattcaaat tatctactac aacatctttg aacaatgcac ctgcatcaaa
    25261 agcaaaactg aaagcagaac cttcagattc agtaaatcca gcacttgggc caaggtagga
    25321 atataaaagc ttgaaattgc ctcctacccc tattttatct ggtactactt catatccata
    25381 acctaaagct ccagacactt cataggtatg aaaatcacca agatcaacat tactggaact
    25441 ggtttgtgtc tgtgttccag catcaagcca gattatatgc gcattaatat tgcctatacc
    25501 ataaaaatag tgattaaaac ctaaatactc atagaagata tcttctattc cagaaccttg
    25561 taaccacggt atatgagttg cagcaatttg agtatctttg ttaaatgcag tagctccagg
    25621 attccaccac attgaatatg cgtcatctgc aattgcagaa taggcacgtc ccataccatt
    25681 ggcacgagca ccaggttcaa aagttaagaa taacattgct gcctgagata ttccagacag
    25741 ggtaaaaggt agaacaagca tcagcattaa gctgaatacc aatatgtggt gctttaacat
    25801 tatacctcca taggctttat aaaacttatt tcaggctaca aaaatatcgc ctgaagaaaa
    25861 ttcctttttt tatatataat ctaatctgca gaatgtaaat atcatcttag attatgcctc
    25921 agttccatca actacatata ggggcttacc ctgttatcaa ttaaatagta ataactccta
    25981 aaaagctttg gtgccgaagg ccggaatcga accggcatga gctaatgctc ggtggatttt
    26041 gagtccactg cgtctaccaa tttcaccact tcggcatatt attctatcca aaaaatcaat
    26101 atacacttta atgtcaagca aaaaggtgtt tttattatcc ccacttgaat tgctgtagtt
    26161 aatatattat aatctatctc ctataaaaaa gcaaatatat aaggcagctg cataatttga
    26221 aatcttggtt taactctcat ttatagaata tagcgataac aatatattnn nnnnnnnnnn
    26281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa aatagttcac gcaagtatga attatagaaa
    26401 caaggattta agaattttag aatttagggg atttagatgt gtagtggagg acgctgttcc
    26461 cctgcaagaa atatcctctc atagattaca cagattatgc atatatgcac aacttcaagt
    26521 tcgagggcat cgcgatcttg aatattacaa ccttttaagt agaatttttg ttcatttctt
    26581 tctcaataat tttagtccac catattgaag atggctcaaa caaacaaccc atccctctct
    26641 cctcctcctc tcctcatcaa cccatagtta aggatgagtg aagatggggt ggatgatgtg
    26701 ttgtatctgg gttaccagca agaaaagtaa gggttagagc aagccaacta tttatatgtt
    26761 gttaatcttc caggtgttag caaatctcaa tggcttcgca ttatcgatct taatatgctt
    26821 tttatcgtga tgataaatcc actatatatt ggaggtggta ttatttcaaa agttggcgta
    26881 aaataaattg ggaaaaccaa ttggatatgt gcaataatgt tatctattgt atggattcaa
    26941 ggtaatctct aactgacaag gaaaattgct ttgacatttt atcctctctt tattataagg
    27001 catttatgtt aactaacaag tgagcttagt aaagctaaac tgcttctact aaggagaata
    27061 caatgctagc taaactctat aggaatactt accgttgctt agttaagttc gagattgaat
    27121 ttgattccct aaaattataa ttggcaggta agcatcgtat cctgcgatta ggatttaata
    27181 atggggaaat tcaactattg cttacggaac tttgtgatgt ttgtagaatt aagatatttt
    27241 aaattatcgg aattgattgc ccagggttcc aaagatgagt aaacaggaaa atgttgaccg
    27301 tagtaattag tactttgacc ctgaatatga ataaaccaca attgaatata tcaaaaatat
    27361 acggtgtaca taatagtgat gaagaatgat gttaagatct aaatatgttg agattattcg
    27421 tataaggaag aggaacaaga tatgctaata ttcaaggtat tacatagtag gaaagaactg
    27481 cctgaaaacc ttactcaaaa ggatatagta gatttccttt acacacattt ggaacgattc
    27541 agagatacat cttttgcaat ttctaaagcc atcgattatg ctttttccaa tgaaccggga
    27601 aaaggtggtt ttgttatttt ggctaataca ggtgataagc tctgcggagt gctagttatg
    27661 attaaaaccg gaatggaaga gtttattcca gagaatattc ttgtttattt ggctgtgcat
    27721 tcagcaataa gaaatagagg gataggcagt gaattattaa agaaagcatt ggaaattgta
    27781 aaaggtgata tagccctcca tgtagaatat gacaatccgg ctaggcatct ttatgaaaga
    27841 tttggtttta cttccaagta tgctgaaatg cgttggcaga aacaatatta gggaacacgg
    27901 attagagcaa aatcaataaa actataacta cagtattaat ttattgccaa ttgtaattac
    27961 cgtctattac tctaaatata aagttataaa tctaaactga gcttccgctt agaaaagaac
    28021 agacaggaag tataaagata ctattgctct ctttatcgcg ttactataga ataaagaatc
    28081 gattgagaga aaaaacaaga caatagatct aattgtttaa tgcaggatag ttatcttctt
    28141 cgtccaaact ttccctgatg catccatgcg taggatataa tttccattgg ctaactggct
    28201 accttgatcg tctttggcat cccaaagtag atgaaaattt ccactatctt tagtctcgtt
    28261 taaaattgtt ctcacttttt gtcctttcag gttataaact tgaatcaagg taggagattt
    28321 tgagggaatt ccatagaaaa ttcttgcttc gtaatcaata ggattcggtg ctatggaatg
    28381 aaatccttct tttactggaa tggaaggagt ttgattgcct atattttgaa tatctgcgat
    28441 agctggaccg tagaatgaat ttgtaccatt gaaatccaca acctccagcc aataatagta
    28501 tatgcctgta gcaaaaacct ggtcagtaca ttggtatgtt ctaatttggg atgtattcgt
    28561 agctggtgtg aaatagccca ggctagttgc agtatccaga tttgactgat tggatcttaa
    28621 gatattaaag cccatcacat ttgtttccga ttgggttgtc caactcaatt tgacagtaga
    28681 gctactagtt tgcgtagcta taaatgaact tagagttacc gggagagtat catccattcc
    28741 tcttccgata tagtaaaata catcatcttt ggcattgaga aagctaagag cgataaaaga
    28801 gatcttcctg ggttgagcag gattccaaat tgttactaat ggctcatcac tgttggatga
    28861 atacactttt ggaaagttat gcacattcca attaaagtaa ttatcttcat cctcattcca
    28921 attgaaggtt atctttttgg tgccgttgaa attacctttc aatatccatt tacggttaac
    28981 aaatgcgtta ttattttgaa agggaagcga ttccgccata aaattgtaca tcaaagtgcc
    29041 atttttgtcg atggaaacct tttgtacatc tggactgtaa tagccatcta ctccttgggg
    29101 cagaatatta taactaaggt ctccgttaag aagttccata gtagaaacta ctgtaatagg
    29161 gttggttaaa gtaacacctg cgagattatc aaatataata tcgtttacag tatagggatt
    29221 tggtgcagga ttaaaatctc cggtttgttg atgaacagtg ccattaaaaa tataattgcc
    29281 attgctgtta taatttcttg tgcctacttg caaacttccg gtttttcctg tagaagaaaa
    29341 gccattgggg ttagcggtgg ctattgaaga actatcttcc atagaaaaag ttcctgaacc
    29401 ccaccatgct tcttcaataa cagacatacc aaaatagaga gtggttccgc tatgaaccaa
    29461 agaagtatat acaagtttga tattcttact taagcttatg ccggtaggat tattaaaatc
    29521 tatacctaaa acctgttccg gtatttttga tccggtaatt tgaggttctg aaccattgaa
    29581 tataaggtaa ggacgattat agatgattgt tccgctacaa gttaaggaac catccatacc
    29641 ttgtggatta gaagtagcta tagtgcttcc gtaattagcg gtaaaagaac aattccctgt
    29701 tattgaacct tggctaccga agatgattat cccattggaa tttgctgtaa cctggcaatt
    29761 ttggctttca taagaattag atataagacc attgttttca agggttccgc catccataac
    29821 agtgattgat gaattggtat taccgttttt tattttacca ttattaatca gttttgaagc
    29881 actgtatatt tgaattgttc caatctcgca attagcatct ttattgattg tcactgcaat
    29941 tccagaatat aattgtaaag ttccagttat aactagacca ccctcaataa ttatagaagt
    30001 atgtatagct atgttctgag tgtttatata gatggtattg gcaaaaggag aagatggcaa
    30061 ctgagtagca ttttcccatc ctgaaccatt ataatattcc cataaactaa ggtcttcaaa
    30121 attaccaccc cattttgtgc gataatctcc tttgttgtaa ctaaataagt taattgtagc
    30181 caaggtaaag atagccataa ctaccaacca tttaatccta gttctttttg ctgtttcata
    30241 atcactgtat ttcataagat aaccccatta aaaataaaga tttaaaataa taacatcata
    30301 ttttgaaggt gaataaagaa tcacaatttt taactaccga taaatgtcaa tcatatttat
    30361 ttgagtgaaa ctaatccttt gtaaaaaatc tttgttgcga aaatactaat agcctaaaat
    30421 aaaaaaagta ttgacgatat tgtggacttt taaaaaaaag gattatatgg agtattatgc
    30481 gataaatgcg aagaattatt gttgccatag acggtcctgc tgcttctggg aaaagcacaa
    30541 cagccaagtt tcttgctgaa aaattaggat acatatatat tgacacaggt gctatgtatc
    30601 gtgcttgtgc tttacaggca aaacttgaag gatttgctat tgatgatctt tcctcaatta
    30661 ttccgatgtt ggaacggata gatataaaaa tatcatattc agaaagtgga aatatcatat
    30721 ggcttaatgg aagagatgta agccagcaaa ttcgtactcc ggagatttct gctgcagctt
    30781 ctaaaatatc tgcaataccg agtgttcgtt ataaaatggt ggaattgcag cgtaaacttg
    30841 gaaaagatgg aggcgtagtt ttagagggtc gagatatagg cacttttgta tttccagagg
    30901 cggaagctaa gttttttatg gtagcagatt tggaagtgcg tgcaaggcgt agatatcaag
    30961 agttgaaagc aaaaggtata gaaagcaggt atgaggatgt tttgcaagag ctaaaagctc
    31021 gagatgaagc agatgcaagt agatctcttg caccgttaaa accagcttcg gatgccatat
    31081 tagttgatac aagtaatttg tctatcgaag agcaggttaa taaactctat gaaataacat
    31141 taagaaaaat agccgaactg ggtgaaagca atagtccaaa taatagtgtt cctgatggaa
    31201 ttgaggttta tttggcgcat agctcaggat attgttttgg tgtgaaacgg gctatacaga
    31261 tggcaagaga agccaaagtt tgtgataaac ctgtatatac tttaggtgag attattcaca
    31321 atcctggtat tgttcaggaa ctggaagaaa agggaattca tgttgccaat aatgtttccg
    31381 atcttaaaga cagtacagta attatacgct cgcatggaat aacgagggaa gaatatgaaa
    31441 cactacagaa aaataataat gagattattg atgccacttg cccgtatgta aaacggactc
    31501 atacaatact ccagaaaatg accgcagagg gttatccagt tgttatattt ggtgataaac
    31561 atcatccgga agttattggg ttgcgttctt ttggaaatga caagacaatt gtggtagctg
    31621 aagatgaacc gccacctaaa attaacgaaa atagtttagt attaatagcc caaaccacac
    31681 aaaagattga aaagcttaat gaattggtat gtaaattgct ccctaatgta ttcgaattgc
    31741 gagtgtttaa tactatttgc ttaactacta ctctaaggca aaaggcgaca atagaaatgg
    31801 caaagaaaag tgatgttgtg atagttattg gaggatataa aagttcaaat acttctgctt
    31861 tggcaaagtt gagttcccaa tactgtccta cttttcatat agagaatgaa atgcagttag
    31921 ctggagtaga tctatcaaag tataaaagaa tcggattaac tgccggagct tccactccgg
    31981 aagagatgat cataaaagtt tataatgtaa tattacaaaa aagcgggcac ttcagtactg
    32041 tgaagagtat tgaagaaatc ccattgttta aggaggaatc atgttaacac atgatcataa
    32101 cacagtcgaa actgaagtta aagaaacgga agaaaaaaac cctatattgg aaaccgtttt
    32161 gaacgaaagt gaagacaaga tgtcaatacc aacagttgag gacgaaaaag aagaaccctc
    32221 aattgcaata cccgaagaaa ctataagtga agaacccgag acagatgcct cggaacaggt
    32281 tcaaagtgaa gaaattgctg aaactcttaa acttgataca aagcggaaag agctaacgca
    32341 agaggagaaa gaacatcagg aaatgctttc aatgtatgaa gaatcatttt ccagctttaa
    32401 aaagggtgag attattgaag gcacagttgt ggatatttcc gataaagaag tgcgagttga
    32461 tatcggattt aaatctgagg gtgtgattcc aatatccgaa tttgcctaca gtggaatacc
    32521 agatacaaat tcagtaatta aagtgtatat cagtgaaatt gaaaatggcg aagggaaagt
    32581 tttgctatcc aagaaaaaag cagattacct tatcaatatt gaaagcatta aaagagcttt
    32641 caccgagaaa acttctttaa ccggaataat ccgtcgtcgt gttaaaggtg gaatgattgt
    32701 agatgttcat ggtattgaag gttttctacc cggttcacag atgtctttaa aaccaattcc
    32761 caatcttgat caatttattg ggaaagaact tgtttttaaa gtagttaata ttgatgagga
    32821 tcatcataat ataattcttt ctcgtagaca ggttttagaa gaagaagcag aaattaaaag
    32881 gcaagaattg ttgaagaaga ttgagattga ttctgaactg gatggagaag tgaagaatat
    32941 tacccaatat ggagctttta tagatttggg tggtatagat ggtttattac atcttaccga
    33001 tatgagctgg gggaaaataa atcatccttc cgaaatgctc cagattggcg ataaagtgaa
    33061 agttaaagtg atcaattttg atgctgaaac caatagaata tcccttggct tgaaacaatt
    33121 agtacctcat ccttgggaaa atattgaaat taaatatcct gaaggaagca gagttacagg
    33181 taaagttgtg aatgtaaaat cattcggtgc ttttgtagag cttgaacccg gagtggaagg
    33241 tttggttcat atctcagaaa tgagctggac aaaaaagatt accgatgctc gtaagtttgt
    33301 gaaagtaggg gatactatta atgccatagt gcttgatctc gataaagaaa ataaacggat
    33361 ttctttagga atgaaacaga tggatcccaa tccctggctt acaattgaag atcgctatcc
    33421 gattggaacc gtgctcaacc gcaaaattaa aagtttaact gcttttggag cttttgtaga
    33481 aatagaagaa ggtatagatg gcttgattca tatttctgat atgagctgga caaagcgtat
    33541 ttaccatcct cgggaagtgt ataaaagaaa tcaagaagtt gaagcagttg ttctttcaat
    33601 agatagagcc caacatagaa ttgctttagg cgttaaacaa atggttcctg atccttggga
    33661 gacattggat caaattcttc ctgttaatac tgaggttaaa ggtaaagtaa gtaaactaat
    33721 tccgaaaggt ctcttagttg atattcctat tggcaacgat gtagtggaag ggtttattcc
    33781 tatttcacat ttggcaattc ccaagcttga acatagcgaa gacgcttttt atgtaggaga
    33841 agaattacca ttaaaagtta tcgaactgga tatggaaaac cggcgtttaa ttctctctgt
    33901 taaggcattt ttcttctcac gcgatcctaa attgcaggat gaatacattg ttctgcacga
    33961 acagtatatg cgggataaag tagcccgaca attgaaaaag aaacaagaaa aagaaatgcg
    34021 cgagaaaaaa ttagctgcta aaaaggatca agaagaggca gaaaaagctg aagaaaccgt
    34081 tgagaaatct ccagagcctg tagctgaaga agtttcagta gctattgaac ctgaaacaga
    34141 aaaaacagaa atacagcaag agcctacaac ggaagaagct cctatagaaa cagaaaaagc
    34201 tgaaatacag caagagccta caacggaaga agctcctata gaaacagaaa aagctgaaga
    34261 aattcccgtg gaaatagaga aagcagaaga agcaatagaa gcggtacaga aagaagaagt
    34321 tatctttgaa ccggaagaaa aaaatgtttc tgaagagcag atcaacgaag aaactattac
    34381 agaagaaacc gctatagatt catctgtatc tgaagaacaa aaggtacctg aaaaagcaga
    34441 tgacgcttct tcaaaaccgg aagaaacatc cgcaccagaa gaagaatcag ataaagaaaa
    34501 ataaactgaa cgggtaaaaa aaactccttt tctccgttta gaacagggag aatagttgaa
    34561 aagaataacg gttttattaa ccgtgttgct tctgctatcg ataaacctga aggcggaatt
    34621 agtaaccgcc ggtaatggga aaaacgaagt aaccttgtta gaggataatg aaaccgaaac
    34681 tattcttcag tacagaattg cagaatttga aaagaccaag atttctcttg agggagaaga
    34741 ttggtatcat attaagctgc ccaaagaagg aataactcaa gataaaggtt ttccggaact
    34801 ccctgttcta aatcggagta ttatcattcc tgatcaggca ttgatggcta ttagggtatt
    34861 tgaggttgaa ttcacagatt atcagcttag aatattgcct tcgaaaggag tgattactcg
    34921 ggatataaat ccagacgaag taccttaccc tttcaacgat atatataaaa ataattgctt
    34981 ttatccgaag gatatagcaa ctttatctga accctatatt ttaagggatt tccgagggat
    35041 tacgatttta gctaatccct ttgcttacaa ttcggttagc ggtattttac gagtttacac
    35101 ttcgtttaaa gtgcgggtat atgtacaggg aactgatagt attaatatta tcagtcgttc
    35161 cagagcttct atttctcgca gtttttacca tatttacgaa aatcaatttt tgaattggag
    35221 cagggaacgc tatatatcgg tagatgataa tttcgggaag atcctggtta tttgtaatgc
    35281 aaactaccta aatcaaatag ctccttatgt tgcttggaaa aggcaaaaag ggatagatac
    35341 ggaactaatt gagttcggct ctataggtac aactgccgat caactaaaga catatatcca
    35401 gagccgctat aacatcgata atgatataac ttatgtacaa ttggtaggcg atgtaccata
    35461 tatacctaca ttattttatg caggaggagg tagtgatccc agtttttctt tagtagctgg
    35521 tagcgataat tatcctgata tctttattgg aagattttct gcagaaacag cagcagaatt
    35581 aacagcgcaa gttaataaat ctattcaata tgaaagagat ttgaacacta cggcaacctg
    35641 gctttcagaa gcaattggaa tcgcttcagc agaaggagga ggttccttag gtgataatgg
    35701 cgaaagtgat ataacccata tgaatctaat tcggacagac ctgttaaatt atggatatag
    35761 tagtgttgac cagatttatg atccgggagc acctgcttct attgtttctt caagtgtaaa
    35821 ttctgggcgt ggttttatta actatgtagg gcatggttca aatacttcct ggagtactac
    35881 tggttttaat aattccaatg tattagcatt aactaacgga aataaaactc ccttcattat
    35941 ggatgtggct tgcgtgaatg gaaattttgt ttctatcacc tgttttgcag aggcttggat
    36001 gcgcaatgaa aatgggggag ctgttggaat ttatgccagc tcaataaatc aatcttggaa
    36061 ttctcctatg tgtgcccagg atgaagttac agacctctta acaagtgaag cgcatacaac
    36121 tttaggaggt ttatactaca acggttcctg taaaatgatg gatgcttata gaagcgatgg
    36181 agttaatatg ttcagaacct ggaatatttt cggcgatgcg tctctacaag ttagatccaa
    36241 aactccctta gcaatgtttg ttagctaccc tatgtctata acttctggaa ctaatagtat
    36301 tactgtctca acaggtgtat caaacgctct tgtatcaata actaattttg aaacaatcta
    36361 tggcattgct acaactaaca gcaatggaat agcagtggtt gatttagata attctcctgt
    36421 aggagaattt acatatacaa taacggtaac agccttcaat agagttacat atataggttc
    36481 cttacagcag atatataatc ccattcctat acaacctcgt tttgtagctg aatgggaacc
    36541 tgcacaagga gcaattattc gttatcctat tggtttacct tactctttta tcgcagatat
    36601 atctaataat ggcttgttgt atataatagt atctaattca ttacaacaat cctgtaattc
    36661 tgttctcact tccaatgggg tgaatatggc taatgtgcgt tatataaaca ccagcagtga
    36721 ttcatactgg ataagggatt atggcccctg gacaattatg gattccagta acaatttaca
    36781 gctggtagat tttacctaca atcgtccacg tcccaacgat gatattgtgc cttccgcgat
    36841 agccaattat ttaggagtaa atttatatca gatgaatcta actcatactg ggggaaacat
    36901 tataactgac ggtcaaggta aagcgatgag cacgaatttg gttctaacag aaaatccttc
    36961 attatctcag agctctatca atcagatgat gaattcttat cttgggatta cagaatacca
    37021 gctgtttgaa gatccaacta atacatatat agatcatatt gattgttggg caaaactcct
    37081 tgatgtagat aaggttttaa ttattagggt tccaacaaac catcctcaat acaatgctat
    37141 cgaaacaatt gtaactcaat ggcaaagtac actatccagt tatggcactc cttatagaat
    37201 atatagaatt gatactccca ataatgagcc atatatcaat tcttacattt taaataagca
    37261 aatctatgtg cctttaatgg gaacagcaaa tgattcagca gcattattaa cttatcaata
    37321 tgctatgcct ggatacacta taacaggtta tacatatagt tcttttgaat ctacagatgc
    37381 tttacactgt cgagttaata ctatttttga taaacagatg ctctctgtaa aattaactcc
    37441 tccaattcca tctcctcttt tacaagaact tcctgttaat ataaagatcc accatagtta
    37501 ctctctaaac ctaaacgcaa cttatgtttt ctggaaaata gggcaaaacg gaacctggag
    37561 tagcagcaac ctgatatatc agaataacga taattggatt tgcaatcttg atgctccgct
    37621 gattggtgat acactatttt actggatttc agcaacggat atcagtaatc gcaatgtttc
    37681 tgctcctctt tgtggagctc aagatccatt tatgatagtg ggaatgtatg ttcctcattt
    37741 aaattcacct caagcgcaaa tgcagattca aggatcatct attagaattt attggaatgc
    37801 tgttaataat gccaataatt atctaattta tgcttgtgat actccagatg ggacatacaa
    37861 tcttgttggt tcaacttctg agaccttttt tatcacactg gcaagtgaga ataaacagtt
    37921 ttataaagta gtagctcaac gaactgtaaa tccccggtga aagctataag aaggtttcaa
    37981 gggtctgtag aattagctca gctacatctt taccaaaaag atcaggatgc tctgagggag
    38041 gtgtaaactt gttgatttca ttagggaagt ctttagaagc aactggtaaa agcaaattcc
    38101 aaccgctttg aaccgtttcc aaccattctg tttcaggacg aagagttaca caaggctttt
    38161 tcaagatata tgcttctttt tgaattcccc cagaatcagt aaaaatcata tcggcattct
    38221 tcatcaacaa caaaaaatcc agataagctt ggggttctat atagtgaata tgatgataat
    38281 gattatcctt tatttttttc agaatattgc gagtgcgagg atgaactgga aaaataattt
    38341 ttctttgcaa ggtattgagt cctgatagaa tatgtaataa gttatcagga ttatctacat
    38401 tgtaaggacg atgcaaagtt aacagacagt agttttcatc ttccaattgc aaagcagata
    38461 aaactttgga ttccttttct gctttcttta tcccaaatat aagtgaatct accattatat
    38521 caccaacctg aaaagtctta tttagcagac cttctttgtg tgcattttgc attgcagttt
    38581 ccgtgggtgc aaataagata tcacttagat gatccgcaac aattctattt acttcttctg
    38641 gcatactttt attaaaactt cgtaaacaag cctctatatg agcaattttt atatttagtt
    38701 ttgctgctgc taaagctcct gcaagggttg tatttgtatc tccataaata atgaccaaat
    38761 ctggtttctc attttctaaa attcgctcta tggcggttaa aatagctgcg gtttgagttg
    38821 cctgacttcc ggatccaaca tttaaattgt aatccggagc tggaatttcc atatcacgga
    38881 aaaaaacctc attcatttga aggtcgtaat gctggccact atgaataata atttcttgat
    38941 ggcgctgtct aatttttcgg gatagtggag cgagttttat aaattgaggt ctggctccta
    39001 caatttgggc aattttcatc attaaagtcc ttatatcctc ttaattatat tatattcttt
    39061 caatttagaa tgttatattc attggtttgc tgtctgtacg caaatgaaaa agcataattg
    39121 gttactatgt aagtgattgt cttcccgcat agaatacgga atccactttg ataaataatt
    39181 taattgtatg gctgtttgtc tgtgcagcat taacaggttg cttttcattc ttaatcctct
    39241 tacagagtca taaatatcaa tacgattcag ctgtcaagaa ctaaggactg attagcttaa
    39301 tgaactacta tgtaaaaaca aaaagctaca gaaaaaaatt tgctaatgag caagattagg
    39361 ttaaattatc catacagtgt cttatcaaag agatgaaaat gaaggagtga aaatgaagaa
    39421 atacttatta atcggtttag ttatgctctg cttttttagt atggaagcga taactaagga
    39481 aacagagcaa aaagaaatca acagagatac aggtatgggc ttccaatttg gaactttatc
    39541 aggaaatggc tttgcgtata ggaactattg ggaaaaatgt ggtttacaaa ttgttttggg
    39601 gggtatgact actggctcga aagatattga ttacgagtta tatggttatt atggtggtgc
    39661 ttctgaaata acaaaaacag aaaatggacg aaaaaccact atcagtacat ctatgaatta
    39721 tttacatact cttgctaata acaatatagg tagattttat gcattcgggg gtggctcttt
    39781 aatatactct cggattaggc aatttgaagt tgattatcgg cgaagttcca ataattatta
    39841 tgtgcggata ttagaccatc ctgaaaggaa aaaatgggat aataaatatg cttactattt
    39901 tgggtttggt ttaggttttg attttgatat cgggcaaaac ttccactggg ttttagaatt
    39961 acctattaca attgatgagg atagtgaaat tgtaatgtac atacctcaaa cagggttata
    40021 ctactttttc aaataaatta aattaaagcc cggtttataa taccgggctt tagtgtttat
    40081 aatattagga ttgttgggaa agcataggat tgctctttct ttccaatttt ttctgtgcta
    40141 ttttccaata atttttatca gaacccgttt atctcgcata ccgtcaaatt caccgtagaa
    40201 aatctgttcc cagtgcccaa aatcgagttc accgttggaa acggcaacca caacttccct
    40261 ccccataatc tgtcttttca aatgtgcatc agcattatct tcaaaaccgt tatggtgata
    40321 ttgggaatga ggtttttcgg gggctaattt ctccagccat atctcaaaat ctgagtgcag
    40381 accttgttca tcatcattga tgaatacaga ggaagtaata tgcatagaat taaccaggca
    40441 taaaccctct tttatcccac tttcgtgtag aacacttctt acctctgggg ttatatttat
    40501 ataatccctg cgtttatcag ttagaatatg cagttcttta cgataactct tcatagacaa
    40561 agaggtagtg aatccaaata ggcagacatt tgagtagggc tatcaaaaat caaatcagct
    40621 cctgcttctt gaaggtcttt tttattccca taaccccagg aagcgcctat agcaatcata
    40681 cctgcatttt tagctgtttt aatatcaact acgctatcgc cgactatagc acagtatttt
    40741 ggctttactt tcattctttc cgctaattct aaagctactg tggggtctgg ttttctgggc
    40801 ttatcaggtt cttcaccgct ataaacgcca aacggatttt tagtatgacg gatcatcgca
    40861 cccctgaaga aatggcgaat catctttttt gtaaaatagt ggggtttatt ggataaaata
    40921 gccagtttga ttttacgagc aacggcaagt tgaatcagat ataatactcc agaaaatata
    40981 ttggtatgaa gataccattc aatatcgtaa ttatcccaat actttttggc aagggcatca
    41041 atattttcag gggtcctttt atcttcaggc agaacttttc ttgccaactc catctggcca
    41101 tctccgataa aactcttata ggcctctacc ggatgttccg ggtagccaag ttctttcaaa
    41161 gaagcattca ttgcattggc aatatctact atgctatcga ttaaagtgcc atcaagatca
    41221 aagataatgg ctttaatttg aggtttgtca ctcattatac acttcctttt ttatttagtt
    41281 tgaagatata ggttaacact taatatcctc tgcattagca attaagatgt gagggaacgg
    41341 aatttcgata ttttcttgtt taaacctttc tttaatagcg atcattaaag atgtttttaa
    41401 atcaaagaga tcttctctct tagaccagac tccgaaaagt atattaattc cggaatcagc
    41461 gaatttatca accaaaaaca aaggtgctgg atccgttaat gcctgaggct cattttttac
    41521 aatatctaag agtacatccc taactttttg cagatcatca ctataactaa ccgttatatt
    41581 aacatctgcc cgcctgatag gatatcgtgt tatgttgatg acttcagttt ttatcatcgt
    41641 ttcatttgga actcgaataa aacggttatc tgatgttttc agctttattg ataaaagatc
    41701 tatggattca attgttccaa tattggaact aacttggact acatctccca ccttaaatgg
    41761 cttttccgaa attaaaaata taccactgat tatattggaa aaactagttt gcgatgcaaa
    41821 accaatagct actccaaaaa taccagctgc ccctaaaata gctgaaagat ggaaaccaaa
    41881 ttcgtttaac agcgttacca gtaaaatcag aacagctata taatatacgg tacgaataac
    41941 taattgttcg ctttgaggtg aaagacggtc tttcacaatc tttttggtaa tagccctaat
    42001 taatctaact ataggtattc caattaccaa tattagaaca actctgatag ctaaactaat
    42061 cttttccggt gttataaaat cggttataag ttttgctacg cttacaacaa ctggcatttt
    42121 gggacctcac ttattttttg ggtgcattct catataatat aatctttttt agtaatatgt
    42181 ccaataatat tttaactgtt ggacacataa accatattga ttcattggtg tctctgcttc
    42241 tgtaaagtgt aagaatattt ctacttaata cagtaaaatg tggggtttac actctaaact
    42301 acaatttttt ttcatagcta acacagccaa ctatgcaata aagcaaatag cgttccggag
    42361 aatcaaaata tttagtcaaa ttgaacttta ggagctttct gagcgctatc ttctgcttgg
    42421 aagaattgaa atggttttac ttttattatg ctggcaatga tattatttgg gaaagttaca
    42481 attaactgat tgaatttctt tgctgaatcg ttataacgca ttctttctgt tctaatccga
    42541 ttttcaatac cttccagctg agcttgaaaa gttaagaagt tttgatttgc tttaagctct
    42601 ggatagcgtt ctacaactac cattaaacga gaaagagcat cactaagacc tgcttgactt
    42661 tgttgaaatg cttgaaatgc ctgaggatct gttaaggatt cagaagtaag atttatattc
    42721 ccaccagctc ttgaacgagc ttccgtaact tgagttaagg ttgacttttc aaaattagct
    42781 gcgccctgaa ctgtttccac taaattagga ataagatcat agcgagtttg ataaacattt
    42841 tgtacctgag cccaggcagt ttctacattc actttttctt tttgaattgt attgtaacgc
    42901 ccgataaacc atcctgcaag tattataatc agcagaatga caacgagtaa gataatgtgt
    42961 ccttttttca ttatgtctcc aaatattgtt ttgactcatt tcctgaaagc agcatttatt
    43021 gtccagattt tttttaactt ccgcaattaa aatgtccatt aagtattata aatatgccat
    43081 aatcgatcta tttagaaagg aaaaaatgag aacaacatcc taatatttca tcccaatcta
    43141 gttcatatca tgagaaggta acaaaacttg gattcgttat tccccaatat tgaggtatag
    43201 taaaacaagc ttaatagaat gtaaagaaaa attaacgctc accttataaa atcttaacat
    43261 taaaagagta taagtaatat agaggcgaaa gccaaaaagt ggtaaggaga taaaatgaaa
    43321 cagctgagtt tgatgcaaaa gcttgtttgt atgggattgc ttataatccc attcggatta
    43381 aatggattag aaaccaaatt gagcggagaa ttttggggac gctggctttc cgaaatagcc
    43441 aaacggaaag acagtaatgg caattatgag gataaattag ttaacaatta tttttcactg
    43501 gaaaggggat atctaggctt ggaaaccaaa tttattgatc aagtaaaagg gcgttttaca
    43561 gttgatgttt ttacaactga taagacctat gaatatcaaa cctattcttt agaggacagt
    43621 actctgatat cttcctcaaa atctggaagt attgatggtg taggactaaa aataaaatat
    43681 gcctatgttg attttggtgg tttgattcca attaaagatc tttcattaac tgcgggaatg
    43741 caaaaagtgt attttggtag tatttatgac tggaactaca ctttagtaag caaagcccct
    43801 tccgatgaac ataaagtagt caattctgcg gacttgggaa ttactgtaaa tggttatctt
    43861 ccttctggtt atggagaata tgcttttggt atttataacg gcgaaggata caaaaaagta
    43921 ggtgccaatc taaaagacaa tactgatttt gcctatttag gtaatttgcg tattaccccc
    43981 ataactggtt taacagttgg tggttcctat atgtataaca cagtaggaag ggacaaggat
    44041 ttgaatggag aagtcacaaa tccatcttat gaaaagcaac agctaatgga tatatatacc
    44101 tgtttggcat atggccccgt aaatctgatc agtgaatgta tcataaaaaa ggttgattat
    44161 cctaatcttt ctgatagcaa taatactaaa gaatatacag ccagaggaat atcaatattc
    44221 cctatactaa atttgaagga ttttgtgaaa tacgatcttc agctggttgg tggatatgat
    44281 atatgggatg aatcggatag aattgaaact gacaaagcaa gaaataaagt aatcgcttct
    44341 acaattggtg ttaattataa ttttatgcca gatgaaacat atacgcctgt tatgcaatta
    44401 caattaaatt atattgataa aaactacgat gaagataaat ctcacagcga ctatgcaaat
    44461 ggtagaaggg attcttcaca aatcatttta caactaaaat ggaaatttgc caacatttta
    44521 taagcataaa ggagctaaaa aatgaagtca ataatcaagt ctgtcgcccc aatgttaatg
    44581 atgttacttg catttagttt aaatgcagaa acgaagaatc aaataacttg cacaggttca
    44641 actacggttt tgccaattgc tcaagcaact gcagaggtct ttatgaataa tcacgcagaa
    44701 atcaatattt cagttagagg aggtggctct ggagtaggaa ttgcttcttt gcaaaataaa
    44761 actaccgata tatgcaattc cagtaggcca ctaaaatcca aggaaatatc aaccgctaaa
    44821 gcaaaaggga taaacccaat tccttatgct atcgccaacg acgctattac cattgttgtg
    44881 cacaatagta atttgttgaa caacctatca attaagcaaa taaaagatat atatacaggt
    44941 aacattataa attggaagga gttaggagga gcaaatgtac ccatagtagt tatttctcgc
    45001 gatgttgctt ctgggactta tgaagtattt aacgacaaag tattagcagg agcaaaagta
    45061 attggtagtg cacaattact ggcttccaat aatgcagtta tcagtgcagt aagtaacacc
    45121 cctggagcta ttggatatgt gggtctgggc tatttaaatg ataatgctaa actacttagt
    45181 gtaaataaag ttacaccttc agaacttact gttaaaaata acagttatcc tctttcccga
    45241 aaactgtata tgtacactaa tggcaaagca aaaggcattg ttgctaacta tatcggtttt
    45301 attcaaagtg atcgtggaca aaagattgtt gtagaacaag gttttataaa tatcaagtaa
    45361 agtgaacgaa agaactgtgg gtagaggtta accctctgcc cttcattaat attctgatga
    45421 ggtctatgat gaataaaaaa tatagtgaaa aagcatttca agttcttact tacagtacat
    45481 ctttatttac tattatagct ctcttggcta taatatacag cttgtttaaa gaaggcttac
    45541 cactttttaa caaggttcct atttttgatt ttctgttcgg ttgcaattgg tatccaacaa
    45601 atgttgagcc tgaatttggt gcctggacac taatcgttgg ttctttatcg gtaacgattg
    45661 gtgctttact tataggaata cctttaggtt tagggagtgc aatatttata tctgaagtag
    45721 cgggaaactg tttgaaggaa atagtcaaac ccataataga attattggca gggatccctt
    45781 ctgtggtata cggacttttt ggaatgtcct ttctagctcc gcaaattaga aattggttaa
    45841 atttggatac gggtttgaat atattttctg cttctgtaat tttaggaatt atgattgttc
    45901 ctatagtagc cagtatgagt gaagatgctc tttcaagtgt acccaaatct ttgcgggaag
    45961 cggctttggc attgggatct acaaaaatgg agtgtatttt tcttgttatt attccagctg
    46021 ctaaaagagg tatattggga agtattatgc ttggtgtggg tagagccatt ggagaaacaa
    46081 tggtcgtttt aatggttgct ggaggtagcg ctcaaattcc tcaaggggtc ttttcttctg
    46141 tgcgcccgtt aacttctaca attgctgctg aaatgggaga gaccgttatc ggaagcttac
    46201 attatcaatc gctttatgca ttagcaattc tgttatttct aataactttc ttttccaatt
    46261 tgataacaga attcttcatt ctaaaaaggg agcggaaatg atcagaagga aaattgttaa
    46321 taacatagga ctcagcttac taacacttct gctatgccta acaatattgg tattactcat
    46381 atttctagtc cgaattttct atttaggtgg gaaggtttta agctgggaat tcttattcac
    46441 tgcaccaaga aacgcaatgc aagaaggggg cattttccct gccttaatag gaactttctg
    46501 gcttactgta tgctctatga tgatagttat tcccttaggg gtactaactg cgattttttt
    46561 aaccaactat ggtaaaccga aatgtttggt gaatattgtg caaactgcta taaatactct
    46621 ggctggaaca ccttctatca tttatggctt atttggaatg gctatctttg tgaatctttt
    46681 acggtttaat gtctcattga tttcaggggc gtttacttta gccatattag ccttacctct
    46741 ttttataact tcctgcacag aggcaattaa aagcgttcct atagattttt atgaagcatc
    46801 tcttgctttg ggtgctacca aaaaacaaac tataatgaga atagtattac caactgcatt
    46861 acccaatatt ttaaccggta taataatttg cattggaaga gttgctggag aaactgctcc
    46921 tattatgttc acggcagcta ctttttatac acggcggtta cccaaaagtt taagcgatga
    46981 agttatggct ttaccttacc atatatatgc tttaatgacc gaaggaactc atccagaata
    47041 tcagaaatct attgcttatg gcaccgctgt tgttttatta attatggttt taggtgtaag
    47101 cgctacagct atattaattc gttcttactt taggagaaaa agaaaatggt aaatgaaatt
    47161 ttaactacag aaaatctgga cttgtggttt ggtcagaatt atgtgttgaa aaatgtgaat
    47221 cttcaggtat tggataaaag agttacagca attatcggac cctctggatg cggaaaatca
    47281 actttactac gctgttttaa ccggatgaat gatctgattc ctgaaatccg gataaaaggg
    47341 aaagtaaaac tattcaatga agatttatac gcgccgaaaa cagatgtgac acaaatccgc
    47401 tttagagtag gaatggtttt tcaaaagcct aatcccttcc ctaaaagcat atataaaaat
    47461 gtggcttacg gcttacaaat acaaggtaaa tacacaaaaa acgaaattaa cgatagagta
    47521 gaaactgcct taaaagcagc ttggctttgg gaagaagtaa aagaccgttt acacagtccg
    47581 gccttagcat tatctggagg tcaacaacaa agattatgtc tcgccagagc tttagctaat
    47641 aaaccagaag tgattttact tgacgaaccg acctctgctt tagaccctca atctactgca
    47701 aaaattgaag agttatgttt ggaactgaaa aacaatgtat gtattctaat cgttactcac
    47761 aatatcgcac aagcaggtag aatttcagat tatactgcct ttatgtattt gggagaactg
    47821 attgaatacg gagctacaga aaaagttttt actgttccga aggacaaaag aaccgaatcc
    47881 tatttgactg gtgttttcgg ataaagtact aaggaggata aatgctttct gatagaatac
    47941 aagaactaaa aaaaatgctg atgaaagaag cgggcttagt agaaaaaatg gtagctttat
    48001 ctatggatgg cctattttat aataataccg gttttctggc tgaagtaaat aacttcgaaa
    48061 accaggtaaa ccaaatcgaa attgagctgg atgataaatg tttcactttg attgctttat
    48121 atcaacccga agccaaagac ctcaggaaaa ttctaatgat ctatcgcatt actaacgact
    48181 tggaacgact tggcgatcta gcagttaata ttgccgaaag tgctgctgaa ctttatagca
    48241 gtcctgtttt aaaacttgtt ccagagcttt ttactatgaa agaagctgcc ttaataatgt
    48301 taagagatgc cataattgca tttacagcag aggattatat cctttctcgc caggtctgtg
    48361 atgctgataa tgaagttgat aactataata gatacatata taagaaaata gcatcactta
    48421 taagagaaaa tccttcacaa acaaagctct atttacatat attacgaatt gctaaaaatc
    48481 tggaaagaat tggcgacctc tctaccaata ttgccgaaga taccattttt ctggtgcagg
    48541 gaaaagtaat taaacataac attgaaacta aagaagaatc aatataatac tatagattat
    48601 ggctaaaata actactgatg tctatatcca tgtttgtata tatcctatcc accttattag
    48661 gaaacctcaa ggatgcttca tatattgtta ggagatcctc taacacttcc caaacgatcc
    48721 cttaatgaga ttatggagat gttactgaga ttttttggga ttattattga ataatactag
    48781 gaaaatcagt tgttattgta gatgtttttt taatctacgg agggcttttt tcttgtgctg
    48841 tttaactctt tctacgctga tactcattaa ttcggcaatt tctttcagtg ttttattttc
    48901 ctggaaagaa agtgtcagta tgtttatttc aattgaaggc atatcggagg ggaaattaaa
    48961 actgaactga gggtattctg tattttcttt tctaacattc aggttataat tgctataatc
    49021 aggattttga tctatgttat tatcggtagg gatattttcg tgtaattcct cttccaagtg
    49081 tctggtatct ttttttagag cttcgcaaat ctgttttttg atccagtaac tggcataagt
    49141 agtaaatttt acattttttc caggatcata gtgttgagac gctttcagta aaccaattaa
    49201 tgattcttgt ttcaaatcct cccaatccag ccctttattt cgatatattc tggcaacact
    49261 ataggcaaaa ttgctatatt gcgtaattag cttatctgtc tgtgaataat tcataacata
    49321 aagtgtaaga gattattcca gaggaagata gatgctgaaa gtggtgcctt ttccgagttc
    49381 actttgcaca gttatcctgc ctttatgtaa ttgaatgatg tgttttacaa ttgccaaacc
    49441 gagtccagtt ccactcttgg ttttattccg ggagggatct gctacataaa agcgttcaaa
    49501 aatacgaggt agatggatat tatcaattcc tttaccagta tcggagacct ctataaatgc
    49561 ttccttctgt attaatttac tcttgatggt tatgcctcct ttggttgtat aacgcagtga
    49621 attctggaca agattgataa aaacttgttc cagtttaaag gggtcacaaa ctaaacgctg
    49681 tagattatca tccagttcaa gttttaaata tagccctttc tcttcgataa tcggggataa
    49741 aatcagcgaa atattattca ggaaggtgat taagttgatt tcttgaagtt ctattgaacc
    49801 gcttcgttcc agacggatta gctgttctaa atcacttatt aaatgaatta accgcttagt
    49861 atgattttga atgatgtgca aatatcttct gtttgttaca ttaatgctat cttccattgc
    49921 ttcagcaaaa ccactgatcg cagttaaggg agtgcgtaat tcatgcgcca aattaacgat
    49981 gaaatccttt ttcatctgtt ccacctgacg gatttcatca atattctgca aaatgaaaac
    50041 ccgtcgtttg gcaacatcat tatgggtgct ggaaaataga taataatgcc cttctatttc
    50101 tgcttccttt aaaagtttat ctttactgtt ttcgctattt ttgataaagg ataataacac
    50161 tggatcgcga ataacttccc aatagtaaat ttgctgcttt ggattaattt caccaaagag
    50221 ctgctgaaag gattgattaa tccattccag tttgccttcc aggttttgag accataaaat
    50281 atcgtctatg gagcttagaa ctaagcgcag ttcttcacgg tgcacagcca aatggtgaat
    50341 ggttttatcc agcttttcca gcatcctgtt cagatcttct tgcaaagtac tgaattcttt
    50401 tgtatttagt tcaggaatgc gtatggtatg atttcctctg actatttgtg cagttgtttg
    50461 taagataagt gagaattgtt tttgtaaaaa aatatggaga atccaaacaa aaagtgcagc
    50521 tataataatt gttataatta atatcagtac aattggaact ctggtcaaaa tcaaaataaa
    50581 ccctagaaat ataagaaagg caaaagcaaa aatcagcaaa gaagtgatac tagtcttttt
    50641 caatgtcatc ttctatctcg ctaagaaatt gataacccac tcctctgata tttttaatca
    50701 gataagcata attacccaat ttttctctta aattgcggat atgcacatct atcgtgcgct
    50761 caataactat cttatcattg ccccaaagat aatccaaaat ctgactgcga ttataaaccc
    50821 aacctggtct tttagttaaa agttgcaata ttttaaattc cgttaaagtg agatcaaggc
    50881 gctttccttg tacccacact tcgtaacggt taaaatcaat tatgaagttt tcagttatgg
    50941 aaagaacatt tttgtcgctt tcccaatcgc tgcgtcttaa gatggctttt atcctggcaa
    51001 taagttctct tgtttcaaag ggcttagtga tataatcatc agcaccatat tccaaaccct
    51061 tgatcttgtc ttcaaggtct gttcttgccg tgagcattaa aatcggtatt ttctgagttg
    51121 ctttgtcatt cttcaattgg cgacaaactt ccatcccatc aatatcggga agcatcaaat
    51181 ccagaataag aagatctgga tgttcaattt ttagcaaatt caacattggt agggctgttt
    51241 caaaaccctt tgctgtaaaa cctgcggaat tcagttgcat tctgataagt tccagaatgt
    51301 ctctttcatc ctctacgata aatatttttt tatcttgtgc atccataaaa aacctataaa
    51361 taatttaact aattttgagc aagaaaaaag gactaataat tggttaaatt ttcttaacaa
    51421 atcgttcaga tggttctatt cctgaacgga atctcaaaat cttatgagac gaaatgtatg
    51481 tatttgatac ttgccaaata tgcataaaca attatcttat tcaatatagt aaataatata
    51541 gaaaaaggag attataataa tgtccaaata tttgattgtc ggtggtgttg caggtggagc
    51601 aacaactgca gctcgtttaa gacgcttgga tgaaaaagcg gaaataatta tgtttgagcg
    51661 ctatagctat atatcctatg ctaattgtgg tttaccatac tatattggag gcgtaatcaa
    51721 agaaagggat cggctgtttg ttcagacgcc ggaaagcttt aaagctcggt taaatgtaga
    51781 tgttcgcatc aatcaagaag ttattgctat caatagaaag gaaaaaacgg ttacggtaaa
    51841 aagagcagat ggtagccaat atcacgaaaa ttatgataaa ctggttcttt cacctggcgc
    51901 agaaccttta attcctcctc tacctggaat atccgatagt gctattttta ctctgcgaaa
    51961 tgtttccgat actgataaaa ttaaggagta tattgaaaac cataaagctc agcattgcgt
    52021 tattattggc gcaggattca tcggattgga aatggcggaa aatttgcata atttggggat
    52081 gaaagttact ttggtagaaa tggcggaaca ggttatgacc cctttggatt atgaaatggc
    52141 ggctattgtc catcaacact taaaaacgaa agaagtggag ttttatctta aagatggtgt
    52201 ctcatctttt agtagaaatg aaagtggctt gcaagtccat ttacagagtg ggagaacaat
    52261 aaatgcagat atggtattat tatccattgg agttcgtcct gatagtaaac tggcagaagc
    52321 agctggtttg gaattgggaa taaacaaagg aataagagta aattcttatc tgcaaaccag
    52381 cgatcctgat atttatgctt tgggagatgc aattgcgtat cccaatccga ttaccaaaat
    52441 ggaaagctca acttatctcg cgggacctgc aaataaacag gcacgcatcg tggctgataa
    52501 tatcgttaaa ggtaacatcc gaaaatataa aggcagtatt ggaactgcca ttgccaaagt
    52561 ttttgatatt actgtcggtt caaccggagt atcggaaaaa gttctgaaag atgcaggaat
    52621 agattatatt tcttccatca tacatacttc ttctcatgca ggttactatc ctgatgctct
    52681 gcccataact ataaaaatcg tttttaatcc taaagatggg aaactgctgg gtgcacaaat
    52741 tgttggtttt gacggcgtag ataaaaggac agatatgctg gctcatatat tgaaacataa
    52801 cgagaccatc tatgaaattc aagaaattga acatagttat gctcctccat tttccagcgc
    52861 taaagatgca gtaaatattg ccggaatggt agctgataat attatcaacg gtttagtaaa
    52921 tattattcat tgggatgatt tgagttcctt ggataaaaat gataccttta tcattgatgt
    52981 gcgaactccc gatgaaactg ctttgggaac aatagaagga agtataaaca tccctttgga
    53041 tgatatacgg tcaaggttga aggaaatacc tgaaaataaa caaatagtgg ttttctgcgg
    53101 aaccggtctg cgttcatatt ttgccgcgcg cattttaatg caaaacggct ttaagaatgt
    53161 ttataatctc tccggaggat atcttactta tgaatacgct actcagaaac aaagtaacga
    53221 ggatatcttt gcggatgatt atattgggaa agatgataat atctataaag gcaaggaaga
    53281 aaaaatggaa agagatataa aaacaataac tgcggatgct tgcggtttac aatgtccggg
    53341 acctattatg cgtttaaaaa acgaaatgga taaattggcg gaaggcgata ttttggaaat
    53401 ttctgccagc gacccgggat ttgccaaaga tgttcctgcc tggtgtaaaa tgactggcaa
    53461 cacccttttg gaagtgaaaa cggaaggtaa aataataatt gccagaatcc aaaaaggggg
    53521 tggtgataaa atatccaaga gcttggaaag tggaaataat aaaaccataa ttgtcttcag
    53581 tgatgcttta gatagagctt tggctgcttt cgtaattgcc aatggagctg cttcaactgg
    53641 taaacaagta acgatgttct tcactttctg gggcttaaat gttattaaaa aaccaaatcc
    53701 gcctaaacag aaaaaagata tgatggcaac aatgtttagt attatgctgc cgcgctcttc
    53761 tcaaaaacta agtttgtcaa aaataaactt tgctggaatg ggtcctgttt taatgagaca
    53821 aagaatgaaa actaaaaatg tggattctct cgaatcgatg ataatgcaag caaaaaaatc
    53881 tggagtaaaa atgatcgctt gccaaatgag tatggatatt atgggggttg aaccaagtga
    53941 attgattgag ggagtagaag ttgggggagt agctacatac ctggcggatg cagaacaatc
    54001 taatgtgaat ctgtttattt gacgattatg atacttttct ctcggtctct tgaagtcaac
    54061 gaaaaaggtg taaaaaactg atatagtaac ctttacacct tcattatgat agaagaagac
    54121 aaaatcaata caaaaaagcc cgtaacgact taccgatacg ggctttatct attttagaga
    54181 gattaggggg tttacacaat accctgatca cacatagcat tggctacttt caggaatcca
    54241 gcaatattgg cacccttaac ataatttaca tagccatcgg cttctttacc attttccaca
    54301 caagctttat ggatattttg cataattcca tgaagttttt catcaacttc tgctctgctc
    54361 cagctaaggc gaatagaatt ttggctcatt tctaatccgc ttgtggctac accacctgca
    54421 ttagcagctt tcccaggagc ataaagaatt ttggaattca agaaaatttc cattgcttca
    54481 ggagtacaag gcatattagc tgcttctgta acacagaaaa caccattatt aatcagtgat
    54541 cttgcatcgt ctccattaag ttcgttttgt gtagcacaag gaatagcaac atcaactttc
    54601 acttcccagg ggcgctttcc agggaagaat tttgcgttag gatatttatc tgcataatct
    54661 tttacacggt cattattaga agcacgcaat tcaaccatat aatcaatttt ttcaccactg
    54721 attccatctt catctaaaat ataaccatcg ggaccagaga tagttacaac ttttccacca
    54781 aggtgattaa ctttttgtac ggcaccccaa gcaacatttc caaaaccgga aatggctact
    54841 ctcttacctt taattgtttc ccctttggtc ttcagcatct cttccgtgaa ataaaccaca
    54901 ccaaaacctg tggcttcagg acggactaaa cttccacccc aattaagacc tttgccagta
    54961 aaaacaccag taaattcatt ttgtaacttt ttatacattc caaaaaggta accaatttca
    55021 cgagctccta caccaatatc accagctggg acatcagtgt tgggtccaat ataacggaac
    55081 agttctgtca taaaagattg acagaaacgc attatttcgg catcgctttt tcctctggcg
    55141 tcgaaatcac ttccaccttt acctcctccc ataggaagag tggttaagct gtttttgaaa
    55201 atctgctcaa aacccaagaa cttaagaatg gaaagattta cacttggatg aaaacgcaat
    55261 ccacctttat agggtccaat tgcactgtta aattgtacgc ggtaaccgcg atttacttga
    55321 acttcaccct tatcatccat ccaagggaca cggaaaatga tgacccgttc gggctctaca
    55381 atacgttcaa ggatattggc tttcacgaaa cgagggttgt ttacataaac atcccaaact
    55441 gtttccacaa cttctttaac tgcctgtaag aattcaggct cgccaggatt cttggctgcg
    55501 actttggcca taaaatcatt gatggtcatt agttcctcca taactgattt ttattttata
    55561 tacctttaaa tatagctatc attttatgtc aagtgagaaa aaaagattat aagatagggc
    55621 agcaataata caggattgcg atcctgtaaa attctgaata aataattagc taaaaaagcg
    55681 gaaaacaatt ttcgacgaac gctatcgtta agtaaataaa aggagtacat cactcttaat
    55741 tgccataata atgcaacctg aaacaagatg aagcaattat gagtaattct ctctatagga
    55801 attatcggaa tgtttaaatg gtatattcac agtaatcact tcagaattta gatcctatct
    55861 gaggcaaata aagctcgtct ttatcataat tatatatccc gatatctcta aaactgtcca
    55921 cctatctttc caatagcagt ttagctactt taggagctct taagcgatgt gccacaggaa
    55981 atcctgatcc cagcaatggt cttacataat acctaaaatc atcagtaatg ccatttcctt
    56041 cagtattaat gaagttattg ggcataattc ttgtttttcc agcaatctca cttagatttt
    56101 ccagctgata atttacagag taaaaaccgg ttctctgaat tgaaattgaa ccatccatat
    56161 tataccagat agcataatga gctgcacgtt cacccacttc gcgtgcttca tgctgatcaa
    56221 catcgcttac gcaacctatg aaagaacgtt gtaaataacc aaaagtatca ctccgcaccc
    56281 gtttaatttt caattttgtg cgaaccaaat cactaagtaa atctcctaat gctcctgttc
    56341 ctgaaagttg aatattacca tgagcatcct tttcttgatt gttagaaagc ttggttacaa
    56401 taggaatccc tttatcatct acaatacctt cagaaacagc tatcacacat ctgccgtata
    56461 gatcataaac tcttttaaca tcacttagaa atgtatcccg atggaaagga cgttcgggta
    56521 aataaataag atgaggtcca tcatcaggat atttttgtgc catcgcagag gaagcagtta
    56581 aaaaaccagc atgccgcccc ataaccacac caatatatac tccgggcaaa gcgcgattat
    56641 ctaaattggc accagtaaaa gctgaagcaa caaatcttgc cgcagagcca aaaccgggag
    56701 tatgatcatt cataactaaa tcgttatcga tagtcttagg aatatgaata gccctgaatt
    56761 cataatccgc tttagttgct tgttcattta ctatacgaac tgtatctgca gaatcgttgc
    56821 ctccaatata aaaaaagtat cttacatcgt gtgcctttaa gaccttaaag atttccatac
    56881 agtacttttc atcaggttta tcacgcgtag agagtaatgc agaagaaggt gtattggcaa
    56941 tctgttccaa attgtgagtt gtttcctggg ttaaatctac aaactcttca ttaacgatgc
    57001 cctgaactcc ataaagtgct ccataaacac gggtaacctg tgtaaactta cgtgcttcca
    57061 aagttactcc taccagcgat tgattaatca cagctgtagg acctcctcct tgggcaataa
    57121 caacttttcc ttccagtttc attttaaaat cctccaattg gactaagtaa ttatatttac
    57181 aaaacaatta aaacagagag ccagtaatct gtccagcaga aaatacttgc ttaaaaatgg
    57241 gcattgggaa taatggttaa ttgtaaaaat taaaaacaaa tgtatgagag gatagatgag
    57301 ttacatagtt ttagccagaa aatatcgccc ccaaagcttt aaagaggtct atgctcaaga
    57361 tcatataacg gaaattctcc aaagcgctat ttctacaaat cgcatagccc atgcctattt
    57421 atttaccgga cctagaggtg tggggaagac ttctttggct cgaattcttg ccaaaagttt
    57481 aaattgcgta aatggaccaa ctatcactcc ctgtaatagc tgttccaatt gtgcagaaat
    57541 aacagcagga acttcccccg atgtaataga aatcgacgga gcttctaata ccagtgtgga
    57601 tgatatcaga gaattgcaac atgaacttct ttatcctgcc agtggttcta aatataagat
    57661 atatattatt gatgaagtgc atatgctctc caaaaatgct tttaatgctt tactgaaaac
    57721 cttggaagaa ccaccagaca atgttatttt tatttttgct acaacggagc cgcaaaaggt
    57781 tttgcccact attatctctc gttgtcagcg ttatgatttt aaacgcattc ccattgattc
    57841 catagttcag cggttgcagg aaattagttt aatggaaggc attacgattg atactgaatc
    57901 actatatctt attgctcgca aagcagatgg aggtatgcgt gatgcgcttt ctttgatgga
    57961 tcaagcaatt tcctactgta tgaataacat tactatagat aaagtccgcc aaatttttag
    58021 tgttattccc aatcaaatat accacaattt tcttactctt atctatgaaa aaaatgctca
    58081 aacactgatc aatgaactgc atcagatttt tgaacaggga atagatctgc aggagtttat
    58141 ttccggaatg caggatttta ttcgtatcct ccttttgcgg aaattacaag ttcaaattaa
    58201 agatatcaat caggacgaac aggtaatgtt tgatgagctg gcaaagttat ttggtcaaaa
    58261 taaacttatg tatatccttt cctatcttat tgcctgtaaa acagatttga aaacaagtag
    58321 caatccatac ctcattttag aagctctaat gataaaacta tgcaaactgg atgagatgga
    58381 agatattgcc aaaataatag aaaagctcaa ttacgggtct attactcaaa ctcaacctat
    58441 ctccaatatt tcttcagctc aatcgcaacc tgttcaaagt aaagattcta caaaagaaag
    58501 tgcagtaaaa attccggatg caatttccaa agaggaaact actgtagtca aattgcaatt
    58561 tactcaagat aatttagaac agctctggca acgcatttta actcgcgcaa agaagattag
    58621 ccaaatgtgt gctatcgctc tggaaagtac aaccagtaaa aaagcagaag gtaagaaact
    58681 ctgcctcact gtggaaggga atacatttta caattgcttg aaaacaaatc tggaagctct
    58741 ggaatcctca atttctgagt tattcgaaaa acccgttaaa atatctcttc atcttcaaga
    58801 aacagagcca cctacaaaag taaatatcca acatactacc ctggaagata taaaacagga
    58861 aaatccagaa atagataaat tcgttaagct tactgattct gtgttagtta aaactctatg
    58921 aaaagagttt ttcctttgct tactttgctt tattcagcct ttttaatttg gctaaagcgg
    58981 ggtgtccata agataatctt ggaggataaa aaaaaggttg ttcctgaagt ttttcaagaa
    59041 atctccatca ttattcctgc cagaaatgaa agaacgaatc tgcccaatct gcttaattca
    59101 ctaacccatt tggattatcc cgaaggtaaa tatgaaataa tcctgattaa tgatcatagc
    59161 aatgacggga gtacggagtt tttagctacc caaaatatag ttccaaactt aaaggttata
    59221 gatttttaca ctgagactcc tcccttgatt ggcaaaaaag ctgccttaca acaaggtatt
    59281 gatgaatccc gatatgatat tcttgctttt actgatgctg attgccttgt tcctgtttcc
    59341 tggttaaaag aaatcaaccg ttctttggat aaagatatag attatctgct ttcctattct
    59401 gttttgaaaa gaacgaagga cggaactatc tttcggctaa aaaattttga gagaagtgta
    59461 tattatgctt tagctgcagc aggtttatac tataaagttc ccttcaccag cagcgcttgt
    59521 aatatggttt accgaaagaa agtattttat gaaagcggtg gttttgaagg tattggacat
    59581 ttacgatcag gggatgatga ccttctacta atgaagatga tgcctaaaat cagaaaagct
    59641 gcctataatc cttcttcagc aatgcaggtt gtttcaattg atggcactga tagaaataaa
    59701 catcatcaaa ctaatattcg cagggcttcc aaattcagat atcatccttg gtggctaaaa
    59761 gggctctctg cttttgtttt tatctatttt tgtttatttt atcttgctct gtggcaagtg
    59821 atgagcaaaa agagcaccaa aacaattcag ctttctcttt ttcttaaaac aatagccgag
    59881 tttgcttttt cacaaagtca tttaagacaa atcagtagaa gcaaactcgg ctttttgtat
    59941 cctgtccaaa ttttgatctt tcccgctcag tttatttatt atgctgtgcg ggggacggta
    60001 ggaaaataca cctggaaata attacatttc tactaactta gcttgcacat attccagagc
    60061 attcagttcc tcaatcatct tatccaggat gtctcgatca ccttgtacct ctaaaactac
    60121 caagccatca ttagcacaga attcattgga tacctcatgc aatcctaatc gaacacgaat
    60181 attgcatcca tacttagtta aaatatcctg aaccttggta gcttctgagc tacgctgatc
    60241 gatttttaca agaataacct tgtaaaccat aatatcctcc ctctattttt tcttactgag
    60301 ctttgcccag ctatccctta attctactat tcggttaaaa atcagagcat ctggtttgct
    60361 ttcataatca gtattaaaat agccgattct aagaaactga taccgttctc ctatttgtgc
    60421 cttgtgcaag ttctcttctg ccagacagtt ttgtttaact ataagcgaat tgggattcaa
    60481 gtaatcttta taggacttac cttcttccat attactcata tcggctaaag tgaaaagacg
    60541 atcatataaa cgcaactcaa caggtatagc agattctgca caaacccaat gcaaagttcc
    60601 ttttacttta cgcccatctt ctgtccagcc tccgcgtgat ttttcatcgt aagtgcaaag
    60661 taattctata atatttccct ggctgtcttt tattacttcg ttgcaagtga tataataagc
    60721 gtattttaag cgaacttctt tgcccaaggc aagacga
//
LOCUS       DAOSTH010000006        59997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_560_length_59997_cov_14.340546, whole genome shotgun sequence.
ACCESSION   DAOSTH010000006 DAOSTH010000000
VERSION     DAOSTH010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 59997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 59997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59997
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_560_length_59997_cov_14.340546"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..1462)
                     /locus_tag="PLB65_02105"
     CDS             complement(209..1462)
                     /locus_tag="PLB65_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOQ77348.1"
                     /translation="MPNFAYIIKDAKGARIEGMIKADTLDIAVDKLSKEGSTIISVKA
                     AAEGAFKGKLSLFDKVMLAIYKWRTGVSLKTLVFFTRQLSTMFSAGLTIEKAISDLEK
                     EEKNKKFAKVLRKLSDDIRKGYSLSEAMQQHPGVFNPLYIALVTAGEVSGTLHTVLDE
                     LADYLEKVEDTRRKVYSALAYPVFIIIFLCFVVWGMFYYIIPMFADVYANFHADLPAP
                     TTVAINISNFITSNIFVTILVVLLIILAFFLINLTDKGRYVFDSIKLKIPVIGKVITN
                     SIMSKFSRTFSILMAAGVPIMDTMELTENVVQNAVIESAVRKARVLVKEGYGVANAFR
                     RTGMFPPTLLQMIATGEETGDMDKLLSKAAEFYEKLVDSVIDRLTSLIEPLLIILMAV
                     IVGSIIVVIYLPIFDLGEAMSQGLR"
     gene            complement(1487..3196)
                     /locus_tag="PLB65_02110"
     CDS             complement(1487..3196)
                     /locus_tag="PLB65_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOQ77349.1"
                     /translation="MVYNPQFARLGEILVHEAYVTEDQVKEALVKQSNFGLKLGQTLI
                     KLGYLTENELITALHQQLGYDVVQDRELMDLDIDIVSLIPEPYAVENRVLALREEGDG
                     VVVAMADPENLNVLDSLKKLIGKNIKPVLIADSNLHDAIEKYYKSIRTTTEVEDAVGG
                     FEFVAVDEDENEITIGAATEDVDAPVVKLINLIINEAIKAGATDIHIEPLLKVSRIRF
                     RVDGALREVMTPPIGMHASLISLVKVMSKLNIAERRLPQDGHIALKTSLKSVDVRVSI
                     TPTVLGEKVVMRLLDKGEFGFKLTTLGFESADMDIFRKVIRRPYGIIIVSGPTGSGKS
                     TSLHAALKEIQDIESNIITVEDPVEYRLEGVTQIETKEQIGLTFGSALRSVLRQDPDI
                     VLIGEIRDEETADIAIKFSLTGHLVFTTLHANDAPATITRMIDIGIKPYLVGSSLSLV
                     MAQRLVRKICKYCVKDYIPTEQEIMDAGLTPEEASKINFKIGAGCVHCDNTGYSGREG
                     IFELLTINPEIRSIIYEGGNQDLIRQAAIDNGMRTLHDAALTKMKNGITTIREVIKMT
                     VIE"
     gene            complement(3310..5562)
                     /locus_tag="PLB65_02115"
     CDS             complement(3310..5562)
                     /locus_tag="PLB65_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77350.1"
                     /translation="MKNHSSLMSILTLLMIILIGFSASCDKRNPPPVLPPTQVPVSSS
                     QERYITRITANPDTIYADYNITYSIISVEVKDGEGFGVFNQMVKFKTNLGRVLTDVHT
                     DSSGIARTTFWDDGDQGTATISAIVRNYDASGDTLVWQDSTAINVEIKPIPPIKSVNI
                     KLPSLNDPYLMSVMQTINVIAIPINQDDQQVPNNTLVTFECTKGRFVDSQDNEIGTSV
                     VVATFNGRATVRYNSWTDATTMPGADNAFVTAKVGNASDTKEIIIKPGRPANIDLKTY
                     VRVNDQLIEADTSSVNSPNWIFVKAGLTDIYNNACSYQPVKFTTDLGSFLNTTQTVTQ
                     NTQTDGVASVRFTPGLSAGAASIKASANGDTLFAQTIFMVTSSELYSLDFTQQGQINL
                     NVANTGGLQSAILRVKLRDINGNLIDVPQKVYFKIANTNAPQGANLNGYPQQDSVEVI
                     SNGGEAQVSINSGTDSGIVKIQASWVNSDCTRYIYALKTNIVIHSGPPVPGGIVSFVS
                     GFDSGDNLGGGLWRVQCGAIVKDMYNNPVDYQTSVWFSIINNPGCEIIAQSYVGNTNA
                     DGDSTEGIAYTYINYDGTLTYQTITIRANCGDDPYGTPIFGDTDCILPLNAPEAYMEI
                     QPAVLMFDNNQTSYEYTDTYFALTDGQGCPVKDAQVMLLPLDGGHCIQNPNGVYDPSS
                     PPNEWWMLLTDETGFAKGRVAFKAGECIPPIEGIPQPTPMRIRGTLMDGLLNREVTVT
                     LILFPGQAPW"
     gene            complement(5559..6971)
                     /locus_tag="PLB65_02120"
     CDS             complement(5559..6971)
                     /locus_tag="PLB65_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOQ77351.1"
                     /translation="MKHIRHCKRRYTIILMLSFLACISFLNAQPRRDPILDTKVTFNA
                     DDATLSSVFKALSRLSNTNIVLAVDQAGTTEKEEKRVTINIKDVPIETAVSLVSSAAG
                     LSYRVVSNNTFLVGQKQDIMAEMGERSNIIYLNNLDAKKVSDALQNTTAKITALEGQN
                     ALMIYGNTDTFREVEELVKSIDSQQQQVEIRVRLIEVHLNEARQIGVDWSKLNHLTTI
                     LAEDPVNQYGTGLPYNYSDATGYLPHGNPTDIGVVPDEQYFQRIDGFNDIGHFSRQLT
                     AFDVTIDWLLENNAAQLLTDTRVTALNGELATMHIGEVVPFVVTDKENQLQVERSETG
                     IILNVTPKVNKDGNITMNINPEVSSVTELVGGYVPRTKVRRVSSAVTVPNEHKIIVGG
                     LLNSNITQKVNKVPLLGDLPLVGKIFQHRYELVENTDLIIEITPRLVSMQETSPTVKV
                     DKRLTRTLIQYEEEGEEEQE"
     gene            complement(6999..7541)
                     /locus_tag="PLB65_02125"
     CDS             complement(6999..7541)
                     /locus_tag="PLB65_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77352.1"
                     /translation="MQLRFIKDLTIALIVISLFAMAVQLVGLYRSWEAIPDKSVHTKE
                     SVSDTLLSRIKSIENSIQDRKMFVFNSNKDPLRQGNIIKDKIDREKEYQEMLKNTFRL
                     ATTARDEFGNKIAYIEYQGKLYEARVGDIVEGRRILDIQDKSIRYSHNGIITTTELAP
                     RPAKPVEETTTYGSGTSGNW"
     gene            complement(7543..8094)
                     /gene="pilO"
                     /locus_tag="PLB65_02130"
     CDS             complement(7543..8094)
                     /gene="pilO"
                     /locus_tag="PLB65_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOQ77353.1"
                     /translation="MREKYLIFVLCILLITVLFFVFTINSVSTNRNKIKELDQKIKTA
                     QEQLNSARIMDQQLSQFALIIDNSLTREASFSFDEINAFKTKIGQMADQRTITINKLS
                     DSNKFSFPGLVETTFNLELEATYVQIGQFISDLESMDNILKIHALDISPLQASDKEVT
                     AANAPNRYRVTLEFSVFKVKREA"
     gene            complement(8091..8684)
                     /locus_tag="PLB65_02135"
     CDS             complement(8091..8684)
                     /locus_tag="PLB65_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77354.1"
                     /translation="MENMFYFKINLNKYGEMRLQAEHEKKVFINSIVVFAVGLIIILA
                     SWFYINGMAKARVESRQKYLNELQRELASYETSKDYLSSNDLDKLAETFNNRIFWAKK
                     MVALSNAIDEKLAVRKFSYANGILTLNGITEVNKNIRELDLIQSFIDRLKANEEISND
                     FPRIKSGSITRQVVKDTAILEFVIECYSREVAEGGVK"
     gene            complement(8700..9770)
                     /gene="pilM"
                     /locus_tag="PLB65_02140"
     CDS             complement(8700..9770)
                     /gene="pilM"
                     /locus_tag="PLB65_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOQ77355.1"
                     /translation="MKKKKQVRFKETVGIDIGTHSIKIVHLKKLHEGFKLLNYEISPT
                     IPTGTEYIPSDLGPERYGPVLNEMLKTLKINPKHIKHLVTSIGGDNTSIKQIKTIFLP
                     DEELESALFFEAKKHIPISGSDMILDYQVINLEEKTNNMNILLAATSKDLLNEHTNVL
                     MTVGLSPNIVDIDSLAIANSFILNTFVEDGVYVLLNLGAHRTNMVIWGPEAKFFARDI
                     PYGGYNFTRDIMRKRQLEWNEAEHHKLEFGLGENPAVENIQTVSMLDITEKSTEESIA
                     EEVRRSLRFYVKEAGNSDFRKVYLMGGTAKLKGLKEFIEEKVAIPTEIFMPFINVEMP
                     EKFQDKKDPQLALAIGLAMRLE"
     gene            complement(9809..11752)
                     /locus_tag="PLB65_02145"
     CDS             complement(9809..11752)
                     /locus_tag="PLB65_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77356.1"
                     /translation="MKNILKSQSGNIAIAMLLAVIGAMSGLTMSSLALRNSVAAMSEL
                     EDIQSVHFLRTEADRGQAYLSVAAKRDPDIVGGVRTPTRKIQMNGSNYRKLYSMQSLI
                     NKIYKETDADYVEVGGELQATPTGASITFYQVRSLVEGRTGTSDIYYGRRDLSLTRKY
                     GELTLLQDTGPVFMYFTDSELSPNDTNVYFYGYDVINGPVHCNTDIQIKQLGGGNNNG
                     WPTFLSIVTTSGVVISSPPSYPRDNVFQGGLIENYDEYEFPTNMNEIRAFGSQIGPSG
                     EDRILMVTVDDTQVSGWQGQITTRREHKELYPNYPMGYPYTDPPTTVNYFPTTDTLWT
                     PFSMTAHNGGAYWANCKLWIKGTFKGFQTWASADTMWLIGDLTYTDTALGASPLQNKK
                     DMLGLVSEQSVIIKYAYRDPSDSLRVHTNMGSDGDLDNGIWIYAAMAALGKGVNDRYG
                     DGVFTFEYQHPHGSIPAVKFNPPTDDPDAGMIVFDWIDIHRNYWPQSAAHSWPAWLDF
                     PWYNPIWPEARPYLERGTINIWGGVNQRRRGFVHRSMNDSEYPSNSGVWNPPTDLCGG
                     PCGTTATNIQLYQNPAVGVTLQSRHYPGASGSGVGYKKNYNYDTRMYTVKPPFWPYFK
                     KQGERLPLEQGSWYLKKPPRTLI"
     gene            complement(11771..12412)
                     /locus_tag="PLB65_02150"
     CDS             complement(11771..12412)
                     /locus_tag="PLB65_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOQ77357.1"
                     /translation="MKHIIFNEKGFTLFETIAVITISSILIVTASMGIGVFFRKYQEL
                     NAYVDLQKDMVAFLNVLKYGHHIGSRSSDIEFEGITSAKEITLIRMSSVPGVSHGVEI
                     KPPISDKYPNDFVRYYLHDGVIKMDYRHRGTDSPSAKVIFPEPGLKDRVIIEDFLISD
                     ANANNAIFAYKPDEKMCVINVDLKAKVKIRKDEYRKVHFHTIMAMKNMDRPSP"
     gene            complement(12421..12897)
                     /locus_tag="PLB65_02155"
     CDS             complement(12421..12897)
                     /locus_tag="PLB65_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77358.1"
                     /translation="MKKNIHTLPENNSPNERSFNFSVLGSQGGYSLAEVLVVAIVLAV
                     MVLTVYIGIEFAEKKITQDYRQRVATLLLTGELEKQYTLYMKENIFRPFTEVPVEIDK
                     TEELTVTGMMSISVGREVENYMTHTYGYQYVIGEIRWVDPVTEKVHFVRLREDFYD"
     gene            complement(12900..13955)
                     /locus_tag="PLB65_02160"
     CDS             complement(12900..13955)
                     /locus_tag="PLB65_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOQ77359.1"
                     /translation="MTIHELLRFTAEAGASDLHIAAGAHPMVRVNGRMKKLNLPILSP
                     EEVETLVFGVMNESQQALFKKNLEIDFSTKLSNDVRFRVNAFHQINGVSCAFRVIPNE
                     IKSYEELHLPDILRHLTTKEKGLILVTGPTGSGKSTTLATMIDSINEGRNCHIITVED
                     PIEFVHRSKNSLINQRELGHDTWSFTAALRSALREDPDVILVGEMRDLETVSLALTAA
                     ETGHLVFATLHTGSCTKSIDRIIDMFPKEQQQQIRSMLSESLEAVIAQTLLPTKDGKG
                     RVPSVEIMIANAAVRNLIREEKTYQIPSVIQASTKEGMQTRDQSLYNLVMNNLIERTI
                     AEQFADNPKQFATGAGF"
     gene            complement(13975..14412)
                     /locus_tag="PLB65_02165"
     CDS             complement(13975..14412)
                     /locus_tag="PLB65_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOQ77360.1"
                     /translation="MLRKLKNQKGFTLIEILVVVIIVAILAALAVPRYLRYVEKSRST
                     EAQTAINTIRKTYDIYMQTNGSTDGFSVEQALKEANLGESTVKNWKFEVVGNPPKKYI
                     ATSTQDFAGGEGKQVWYDVTDAQFHGYGIDTWTNPETGGMETD"
     gene            14750..16789
                     /locus_tag="PLB65_02170"
     CDS             14750..16789
                     /locus_tag="PLB65_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOQ77361.1"
                     /translation="MSRSLSFYLGLLLLCINITFLGADNTPKPVSNDKRINETDILAE
                     FDGGVITRKDVEAKISKLPVQYQARYKTVEGQTEVLNAMAMEEVFYKKAQQMGLENDP
                     IVLERIANIEKRFYIQEYYKRNVSDLVVITEDDLQSYYNQNMSQFYLNPYIEIDYLQA
                     ADENEAKKALEELKKGVSFAEVSDKYNQNTYAKGLKGRIKNIRLNGNIPGIGNDYELE
                     NLIRTSKVDSLSFIGPVNTSYGWHIFRTVNWVDGRQKDFSEVKQEIEQRLRPLKEREL
                     LDSIIENSKIKYAVVIDSSLVNTITLDVNKRNNNNPILDNVVLSSSDPDLRFTVRQLL
                     QIYDKLSPQEQQFVIKGGGINLLINQELTQYLMYLEAKAAGYDKYIADNEDYLQAKRA
                     YILRRAYEKLITENIEISDEEIAQRYERDREQFSSPEYRTIEVLFFKDKKTAEKALKK
                     YSKAYKKNNEKAMQDVIKKYSIKPNNAIMDYQYRNGIITGLGDDAKFSNMIWDNPVGY
                     LSPVFTTAKGDIVFFRTIKETPKVYKSKLEVEPRIYGMLKAEKEKAKQAEVTENLYVE
                     FNMKKYPERITLTMTAEELFTQADNAARQRNYRDAIVYYDQIINHYKNNVDDYKASFM
                     KAFLVAEEMKQTDLALQLFREFLQKYPTGDLNESAQFMIDSLEGKIPEHFEPVED"
     gene            complement(17110..17871)
                     /locus_tag="PLB65_02175"
     CDS             complement(17110..17871)
                     /locus_tag="PLB65_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77362.1"
                     /translation="MKWRLIFLCIFCFILGEALFAQTDSLKTSQYINSGELTQLPDSL
                     DKAIRDAEQKKNADLFYNLGVDFFARGDIGMANLYFLKALNINSAHSQAKNNLNLSIR
                     YSPDADLYPEHLFLVQVLYKVLDFFSVNRLAIFVLIFLFLSALALCWLLFYDPEKERA
                     LPILVLALCLIICIASSITLGIKLHHQKHNPNAVVILNQTNLLSPTSNNVIQTVHSGL
                     IVKIVQSENNHYLVHLPNGQLGRLKKEAVKTVIGS"
     gene            complement(17868..19649)
                     /locus_tag="PLB65_02180"
     CDS             complement(17868..19649)
                     /locus_tag="PLB65_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HOQ77363.1"
                     /translation="MKKNILLIMLFPLMLSALKIDLSVNKYVISTNDQLEVTLKITDS
                     DRIKVVEPTPPSIPLFSFRNMTSGSSSSVVFEGTKLVSEFSETFRFFYFPLKTGKTTI
                     PSFQVKVNGRFYSTREINIEVVKGTSKAPSQSAPSYPNFGAFGFDEPDSWDDREQWIG
                     DSFLLAIPQEQTIYRGFPTIVSYYLYTDEMVRSFNLENEIDSDGYGKSTYEQPSVLNY
                     ENVNLNGKQYKRALIKRLAIIPNKEGRLQAPILEGYARLYRFSYSNKTLRSEGGIINV
                     LPLPKNNVPASFGGAVGDFKISYSLSRKELSLGETITFTLKIKGRGNFNQFSAPEFAS
                     GKGFQVSTPMVIDNLKAGIEGERTYYYTLIPQDKGSYELPELSFTWFDNYSGSYKTYR
                     SPKEMIKVSSANVLSYFNRLRQSKTPKAMLPKVVKKHFPPYVPYVQQPWYWILVLLIF
                     AFSLFVAFFALDSKMKRDNPERYASLKAEKVLLRYLKPATLAAKNLSLEFYPLAEKAL
                     FEYLASKYKLSNRLSTKEKLQALSQENIPDWLLEEIQLFLNHSQAARFMPESERAVNL
                     DEDLNRIRKIFNGFTRLGKNKHNRSTK"
     gene            complement(19651..20235)
                     /locus_tag="PLB65_02185"
     CDS             complement(19651..20235)
                     /locus_tag="PLB65_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ77364.1"
                     /translation="MSIKAKRIILLVIVIIILMLILELLLRPKVIRQSFADSFYQAQK
                     YNKAENLFRKNTTNNDAAAYANLAKSLYKQNRFAEADSAISIALNNAQDKKDILYDRG
                     NIAYLQKDYQKALKDYKEALLLNPNDDDLRANYELTLRKLQKQPNPQPLMQQNEDKHK
                     DEDILNILKGLDNKESTDRQQQKPKQSFKTDKWW"
     gene            complement(20225..21220)
                     /locus_tag="PLB65_02190"
     CDS             complement(20225..21220)
                     /locus_tag="PLB65_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ77365.1"
                     /translation="MNIANPYYLALLAFCLIFLILLFRRENRLRRRFQKYAELHLKEH
                     YYHSQSPFWIGFKLFICILALGCIIIALTRPQWDYEKKDLQSSGMDIIFAIDVSKSMD
                     ATDMMPSRLLRAILQIGSFLDQVKTDRIGIIAFAGTATLQCPLTDDYEAVRIVLNGLN
                     SNSVEIPGTNIGKALALAAEAFPTDSKANTLILISDGEDLENSVLRKAKELKAKGIRV
                     YTMGVGSPKGTIIRHPETGEEVKTFLDETTLQEIAELTNGEYFRVTPGGEEIQLILKR
                     IYESESTRRGKKVTILKEQYYLFGILALLLLIIESLLDPRKRKRGLMAAEHSDEH"
     gene            complement(21217..22218)
                     /locus_tag="PLB65_02195"
     CDS             complement(21217..22218)
                     /locus_tag="PLB65_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOQ77366.1"
                     /translation="MIKFGHPWLLLLLLIIPLYLVWELKFNNRRRNYLPHSRVKQLRF
                     LSHNQYLYRYLFPVLRALIIFFLCIAIAEPRWGTRSRDLSNKGVDIIMAIDISGSMLA
                     MDFAPQNRLSAAVSVAKDFVKKRPNDRFGLVAFSEYALTQVPLTFDHLAMLNSLEKLK
                     VNEEASATALGMGLAKAVARLKNSTAKSRVIILITDGVNNTGEIDPLTAAEMAKELGI
                     KVYPIGVGSKGLVPFPYSDPVFGTRYINTLIDLDMETLNKIATTTGTGKAALATDAKS
                     LADIMNKIDRLEKTQFTTQFRYNYSEQFMPFLWLAFIFLAIELLLKTVILPILPDQL"
     gene            complement(22215..23096)
                     /locus_tag="PLB65_02200"
     CDS             complement(22215..23096)
                     /locus_tag="PLB65_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77367.1"
                     /translation="MKKSSVLAFSLIFFPLIIWAFHQELQGADSLKVGTPFLLKITAD
                     YPIHSVSIPDTLEDFAILESRQDERNTRSWQIKIAPLKIGALSFPRIAVNPVDSSVTP
                     VYTDGFRVYVLSVLAEGDTLLRDIKPLQKYPLQLGIWVYLILLLIAIALAIYLWLKRP
                     KKPKREYITQPETKQAILPAWKKSIQALDELIKEDLLAKGEVVLYHFRLSNILRGFLE
                     ETYHFPALEMTGNEIITYLNKRNIPNYREIRAFINYCDMVKFAKRQPSLQEIENYTLW
                     LKDYLISFIGNTIEAKN"
     gene            complement(23093..23977)
                     /locus_tag="PLB65_02205"
     CDS             complement(23093..23977)
                     /locus_tag="PLB65_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOQ77368.1"
                     /translation="MFTQSVSDILKKIRRIEIRTRNPVAEMFRGEYHSRFKGQGLEFS
                     EVREYQPGDNYRDIDWNVSARLGQLYIKKYRETRELRVVFLVDVSSSQEFGTRSMIKR
                     ERIAELVAALSFSAVANQDLAGLILFSDQVERFLPPRKGRNNALEILREVLYYEPKGK
                     KTSLQAACEYAYKMLKKRCIIFILSDWIDSGYERSLKILAAKNEVVALQVVDDSELEL
                     PPAGILSLKDPETGEEIWINSSDPKLRKAYQEVINNEQKELQEFFRECNCDYLMIKTS
                     DSYVSSLRKFFALRGKRR"
     gene            complement(23994..24980)
                     /locus_tag="PLB65_02210"
     CDS             complement(23994..24980)
                     /locus_tag="PLB65_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOQ77369.1"
                     /translation="MLIEAIQNKVEQSSVALENVRLEIARVIVGQQEIIDRLLIGILA
                     NGHILIEGVPGLAKTLIISSLASVFDASYSRIQFTPDLLPADITGTMIYNPKSGDFSV
                     KKGPVFANFILADEINRAPSKVQSALLEAMQERQVTLGDNTFILPKPFFVMATQNPIE
                     QEGTYPLPEAQVDRFFMKLKIKYPSYDEERIILERMVNEQPIPLKKVLSPADIESMRE
                     VMYMVYMEEKLKDYILHLINSTRHPERYPRLNDLKGLIEFGASPRATIYLARAAKANA
                     YISGRAYVIPDDIKAIARDVLRHRIILSYEAEAEEITTEDIINRILDEIEVP"
     gene            complement(25110..25301)
                     /gene="rpmB"
                     /locus_tag="PLB65_02215"
     CDS             complement(25110..25301)
                     /gene="rpmB"
                     /locus_tag="PLB65_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOQ77370.1"
                     /translation="MSKVCDICGKTAQVGNYRSHALNATKRRFYPNLHKVRVEDEKGT
                     KRISVCSSCLKANKVRKVI"
     gene            complement(25366..26481)
                     /locus_tag="PLB65_02220"
     CDS             complement(25366..26481)
                     /locus_tag="PLB65_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ77371.1"
                     /translation="MQKNTKCSNMGFFSGLSEDVLQELTKDLERIQFKKGEAIITEHS
                     WGDNLFFICKGKVEINKGLHNPETPFAQLSILQAGDFFGEMGIIEDSPRSASVVALED
                     VELMIIPREAFHNMIFAYPLIMLNLVRTISGRLRSTNERFVELVDEMIKKNRLMAIGL
                     AASKIIHDIKTPLTVIVLTAQVLENVFPEGREFTENIIKQTKLIDQLVHEILDFAKGT
                     ETPPLIQQVDLDVFFRELQEMYFPNLKERKIDFIVENRVPNYVHFDEGKIRRVLINLI
                     KNAIEAMPVEGEIKILSSISSGWLQISVIDNGPGIPQKVQEDLFQPFVSDGKNYGTGL
                     GLAICKKLVQEHKGRLEYIPIEPHGSRFDIRIPQSAK"
     gene            complement(26547..27401)
                     /locus_tag="PLB65_02225"
     CDS             complement(26547..27401)
                     /locus_tag="PLB65_02225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77372.1"
                     /translation="MKHIRFLLPVILFTLCGTVNAQLAQIIDFFVPQTVPVTPQESFV
                     RYMRGGYLTSGNYYQYLNPDEGFEHIESASVVILHYTYSKFSSQTVPKPLPMVTFGIL
                     DNVAAIQGSFNGDLKNSYFMDVLSLSGSYTYPLLNYNAYLNAGIKTAATYDAPYADKI
                     TALGVEHLFVESIECEYDAANYTYRVTKINQPEPYLHFSAEASARLGVRIYNNWFLAL
                     ALGVRQSPKVEGRWFMKDKVNSWLAGNAYYDTDWDMEQTIDKSLPKEVYFLGGTDFFL
                     CLSVSPFY"
     gene            complement(27611..28570)
                     /locus_tag="PLB65_02230"
     CDS             complement(27611..28570)
                     /locus_tag="PLB65_02230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOQ77373.1"
                     /translation="MKPLLFLTRLLPEPAIKLLEDNFTLKINPYNRSLTKQEIMENIK
                     DADALICLLTDIIDKEIIDAAPKLKVISNYAVGYNNIDIEYAIKCGIVVCNTPGVLTE
                     TTADLTWALIMATCRRITESERFIRSGKFKGWEPMLMLGQDVNNKTLGILGMGRIGQA
                     VAKRAVGFNMQIIYHNDIPLNQNLPYEATEVDFNTLLKEADILTLHIPLTENTYHLIG
                     KNELEIMKDSAVLINTSRGPIIDEQELIRALSTKKIFAAGLDVYEQEPYIPPELLALD
                     NVVLLPHIGSASIETRTKMALLAVENAISVIEGRKPPAMVKLT"
     gene            complement(28567..29403)
                     /locus_tag="PLB65_02235"
     CDS             complement(28567..29403)
                     /locus_tag="PLB65_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOQ77374.1"
                     /translation="MNAINFFKTKKLDLIVDFYTSVLALPVWLNQDSCIIFRAGNQLL
                     GFCRCDISEISGTITFFCKDRKEVDAIYSRLKAIAKTAPKPNLAYNIYNFWAADPEGR
                     SLEFQCFDHQLEPYITLEEGLKTRRSIRKYKSEPVPEELLNKVFELCRYSPTACNLQS
                     YYYIVIKQREILEKIVSLRGPSGKPILASPYAIAVASKGDISHRKIQDACIAAYHLML
                     AAKAYDLGTCWVTDMDKDEIKDLLGIPHQDYIACLTPIGYPAEHFPVPERHQVKDFVR
                     YL"
     gene            complement(29654..30919)
                     /gene="hisS"
                     /locus_tag="PLB65_02240"
     CDS             complement(29654..30919)
                     /gene="hisS"
                     /locus_tag="PLB65_02240"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOQ77375.1"
                     /translation="MKYNIPRGTFDILPEDSYKWQFLQDSFRKFAICFGYQEITTPIF
                     EVSELFERSSGESSDVVQKEMYRFCDKKGREFALRPEGTAPVVRSYVENNLDKLSKRS
                     KLFYLGPMFRYDRPQAGRYRQFYQYGIEFIGSNNPYYDAEVIAISMLWLNSLGLNKLR
                     LEINSVGCPDCAALYDKALQEYYRPFLGKLCPDCQNRFNTNPRRLLDCKVPSCIELRD
                     DAPSQLNYLDEPCEKHFEAVCNYLQAMKIKYIINPRIVRGLDYYTNTAFEIVYEGLGA
                     QNSLAGGGRYNGLIEQIGGKSVPAIGFAGGFERLLIALEEEKINYAEKPKPDAYLVCL
                     GENVRLEVLTYLNDLRIKGLIVDYDPDKNSMKAQFKAADASGAKYAIIIGEDELANKT
                     INLKNLQTGEQIRVAMNAEDTKQRIRAKI"
     gene            complement(31003..31809)
                     /gene="truA"
                     /locus_tag="PLB65_02245"
     CDS             complement(31003..31809)
                     /gene="truA"
                     /locus_tag="PLB65_02245"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOQ77376.1"
                     /translation="MARYLIKICYDGTAYCGWQKQKNGLAIQEVMENALQIFTCEKNS
                     LIAAGRTDAGVHSLGQYAHFDYMGSMKEYQLLLAFNRYLPDDIKVLEIKKVSPALSAR
                     YQAYERSYRYILAKEKNPFNRNYTGFIPYLTLKLERMQAAATYLIGKYDFSSFGRNNP
                     EIPNRMCELKVCDINEQTDYFSFDFVADRFLHNMVRRMVGTLANISHFNLPPETISDI
                     LEKKCPRQNLVITAPASGLYLMNVKYPDKFLDESYLPELQENNGEISDLP"
     gene            complement(31822..32460)
                     /locus_tag="PLB65_02250"
     CDS             complement(31822..32460)
                     /locus_tag="PLB65_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77377.1"
                     /translation="MKFISYPISGQHLYLKLAVFLTGLAIGVDSSLRQLLIQLILILV
                     ILGFEPSLYRLFLNSLKRLLPFFAGYWVFATLFAQDFLTTVFFSIQILYLIAVTVYVL
                     GNVKLKGIAIESKRLRRWKTVNYLFYYLFATIYFIEAFFAEYRNLKQVKGGNLVLAQI
                     RDVLKNVSSLSPEINTSVTDLLSFEREKEIHNYRANYLGIFFLALLVIVHSL"
     gene            complement(32457..34838)
                     /locus_tag="PLB65_02255"
     CDS             complement(32457..34838)
                     /locus_tag="PLB65_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ77378.1"
                     /translation="MKCRVIIICLLCLIGLFACEVNTMYNARNYFKSAQSKPLNSNGR
                     PNAQAIDEYTKAIKKCGKIIATDKKGKRVEEAYYLMAKSLYYKGNSAFQAKDQFQNLV
                     IRFPDSKYVPEAYIYIAKILRETNQPKEAEKLLDEFLRNPKYRKHHPMALSVLADFAI
                     QDKDYIKAQHYLERIINEFPKTKEYREAYFLFGKNYYEQKDFAKSLEAFKKMQNARGI
                     DKAKKLDGTYYIGLNELELGEAEKALKTAKGLLKAESRPDKLPFVRLLKARAQFALGD
                     TTAARSEIELINKNYPRTAGSAGGYYYLAEHYYYNQGDIHKAIASYNRVRTEFSSSPL
                     AASAQVKSSALGLVSPRQGLDCEKNLSNFLDYQYQAAESFLNVLALPDSAIARYRIVI
                     AQKDILVNRCDSLLALISKNNAILDSLRAIVPIQEISEEDSIGIVDKTELLTQLDDLL
                     DVSTFTDSLSNTADSLKITKPEIAAEDSLLKDNIIAIQDSSSNQIDTLVVSIRSEITR
                     LEQENELCQDRINTLEEIIERFDSEIIPFCLFAIGSIYHNNYPESPENAEILTQMQEQ
                     YPENKFTKALYALQNDLPVRLIDPLEEREEQLLELYLEQIYTAPDSALVGLEELTRSR
                     YPKIKLAANYRLGWFYSFEQVDTLLAKPYLNAVLEEPEAGEYATITQRFFNGDIFLLR
                     GAPKFDIPVADSTVVDSTSLNINENKKELPLPEGAEIHKSLSDSLKIYNEIPVLVDTL
                     AKIIPPVENKQEEDISSLETETPEVNTPEPEENPVPTLPEESVPEKKEEIPLE"
     gene            complement(34870..36387)
                     /gene="hutH"
                     /locus_tag="PLB65_02260"
     CDS             complement(34870..36387)
                     /gene="hutH"
                     /locus_tag="PLB65_02260"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HOQ77379.1"
                     /translation="MQEIIIDGNTLTLEAVEQVALYNAPIRLSESSKEKVIKCRAYVD
                     KIIESGEIVYGLNTGFGKFSTVIIPKENIAELQLNLIRSHSTSVGEPYSEVQTRAIML
                     LRIAVLAKGHSGIRLETLETLVQMLNKGIHPIIPMRGSVGASGDLSPLSHLALVLIGE
                     GEAIYKGERLPGSVAMQKASITPVKLAAKEGLALNNGTQVMAALGVLNLLEAERLCRY
                     ADIIAAISIDALKGTPKAFDELIHNVRPHAGQKTSAKNIRTLLQGSPLRESHLNCGNV
                     QDAYSLRCTPQVNGAVRDACAYVRSVLEVEINSATDNPLIFPDEGKVISGGNFHGEPL
                     AIALDTLAMAVCELASISERRIEQMLNSSLSRGLSPFLATHPGIDSGFMIQQLTAAAL
                     VSENKVLAHPACVDSIPTSANQEDHVSMGSISAIKLCQIVQNVASVLGIELLIGCSAL
                     DQRQICSSPVLEKVKASLRENVAPLDGDRIMYPDVNKAIELVRSKRLDEICSEIN"
     gene            complement(36805..38478)
                     /locus_tag="PLB65_02265"
     CDS             complement(36805..38478)
                     /locus_tag="PLB65_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOQ77380.1"
                     /translation="MQKLLQQLNYLNDEHITKVVEVNSHYDPPYMIVEYIHGVSLAKL
                     KTHNPEKLTEDFVRLIAIQIVEAAIKLHQYQLTLSKLALTNVLVKDNNEIVILSSAIT
                     WEERDEREELFSIAGLLAQVLSKSPFYTTIYSQERLANQKFEYIYGVSVPLNKILSEC
                     LHRNIMQRYIGLNEFLDAIINLPPVEKCEIWEEQEKTLPEDMDDISKKEIPKTKIEVT
                     FWVLIILMIGIIVMLLTTNIFSVIFGGKGEKLTYSAWLAPNSESDSVNNPISAKELER
                     NAPLQTSYGELKSGTKSAREDFRKQLPVTNITPSTPSPVVQKPRPPHNMVFIESNSLG
                     LGRLKDNLNHNVSLSSFYMARYEVTQAEWNLYMKPANCSTFGDNLPVDNVSWYDIAIY
                     CNGRSEAEGLTPAYKIRGVGASRVVTCNFKANGYRLPTEAEWELAAKADQLYNYSGSE
                     DPDEISWNRDNSGGKIHTHGTKKPNDYGLYDMTGNVSEWVWDWYDSNYIRSLPTFINP
                     TGPDTGTYKVIRGGSVLNGEGRNLNILWREKGDPNRGYQYVGFRLVRSS"
     gene            complement(38824..41952)
                     /gene="ileS"
                     /locus_tag="PLB65_02270"
     CDS             complement(38824..41952)
                     /gene="ileS"
                     /locus_tag="PLB65_02270"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HOQ77381.1"
                     /translation="MYKTIDLKESPKHLENRIREYWKNHNLVQKSIDIREGSPRFIFY
                     EGPPTANGKPGIHHVMARTLKDVICRYKTMNGFQVKRKAGWDTHGLPVEIEVEKTLGL
                     QDKRGIEEYGIENFCKKCRESVFSYEKLWREMTELMGYWIDLDHPYITLNNSYIESVW
                     WILNNFFSRNLIYKAYKIVPYCPSCGTPLSSHEVAQGYKDVEDPSIYVKFKALEEDNT
                     YFLAWTTTPWTLISNVALAVHPEETYVKVNYHDQNLILAKSRLQVLDEEAQIISEMKG
                     RELEGLRYQPLFDFVTVDKPAWYVCLGDYVTMSEGTGIVHTAPAFGQDDYSLAMKYDL
                     PFLQPVDGEGKFIEAITPWAHKFVKDADKDIIRHLKESGNLFRREQIKHSYPFCWRCK
                     SPLIYYARESWYIRTTEFKEQLIENNRKIDWYPPFVGEKRFGDWLENNVDWALSRDRF
                     WGTPLNIWICEKCGKQRSIGSIEQLRKEGKLISGEDIPEDLDLHRPYIDEVVLTCSCG
                     NKMYRTPEVIDCWFDSGSMPFAQWHYPFENYDNFEPELFPADIISEGIDQTRGWFYSM
                     LAISTLLKGVSSYKSCLVNDMILDKNGRKMSKSHGNAVDPIELMQNYGADAIRWYLLE
                     VSPPWVPTRFDVDGVKEILGKFIGTLKNVYSFFATYANIDGFIASEYPYNWEREVEID
                     RWIVSRLHSLIVTVRNYFDSYDFTKAVRAIQSFVIDELSNWYVRRSRRRFWASGFDQD
                     KIDAYRTLYQVLVETAKLIAPFAPYLAEELFLNLEAGESIHLELYPVSDNKYIDTQLE
                     KKMQSVIDVVSLGRAARNECQIKVRQPLSEMFVPAKIKEDLLDMLDLVKEEVNIRKIN
                     FVSEQDGFVQYELKPDFKILGPKYGKKVQIINNLLNKTPAAEVLMAFNKTGYFAMDIE
                     QETINLFPEEISVSIIPREGYVFANAKDLFVALDTKLTDELINEGFAREIVNKIQFTR
                     KEMNLDIMDNIEIKIVVTPEIARAIQAHQDYILSETLAKEITILKNPLPDMQGIDING
                     HKIYLSITKLNK"
     gene            complement(41958..42323)
                     /locus_tag="PLB65_02275"
     CDS             complement(41958..42323)
                     /locus_tag="PLB65_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77382.1"
                     /translation="MFILEEKHLEAAKEIAQRNRHKKSCDHCYDRGWIGVSEQNLLVL
                     CPKCVDIDKAMEEWKNYVSENEDLKEHFSELFEEKPIETEQDNLQLQKEREHLKSQPS
                     NPPKFVPGQRRTGHQKKIG"
     gene            complement(42453..42959)
                     /locus_tag="PLB65_02280"
     CDS             complement(42453..42959)
                     /locus_tag="PLB65_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77383.1"
                     /translation="MKIIRHFFCEIKRNREPAPQLCAVCPRIIKCKSFRSWNKLYHQE
                     YLNFVVDITKKFPDKYAMEVYFMAEKQTFVQIVDIATGKIEKVVSLTEIESLSAEDKL
                     TLSRNKTLFIVTHRIEPIVKIELKKSVINAPIQFMDNIPKEEEPIEEVTPLPIKPDKP
                     KAKRPTKS"
     gene            43357..43920
                     /gene="efp"
                     /locus_tag="PLB65_02285"
     CDS             43357..43920
                     /gene="efp"
                     /locus_tag="PLB65_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOQ77384.1"
                     /translation="MATMADIRNGMIIEFKDDLYEVVEFLHVKPGKGPAFLRSKLKNI
                     RTGRVLENTFRESDTFNEVRIERTKKEYLYREGDFFVLMDSENYEQMHIDKSLLGEKE
                     KLLTDNMELIVSATPEGEIVGIELPTTVVQTIAECEPNIKGNTASGSGKVAFTETGLR
                     LMVPFFVEIGDKVKIDTRTGEYIERAN"
     gene            43933..44139
                     /locus_tag="PLB65_02290"
     CDS             43933..44139
                     /locus_tag="PLB65_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77385.1"
                     /translation="MGKVKSFAAKVAHDLSTSGKVICPVCNTEVKKIKLITNKKTDGT
                     WAPQKEYVKICKCNEADILAGKNL"
     gene            44326..47460
                     /locus_tag="PLB65_02295"
     CDS             44326..47460
                     /locus_tag="PLB65_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOQ77386.1"
                     /translation="MPCLMWKRIVLLLTIVIAGSLSALVPPVPKYKNLPETWEATSLE
                     GNFYTMDNFKGRKPNLPNNILAIRVQFNDVAFRSEAVYPDNIAHDDIFFDRWMLHLKD
                     FFLEASHLQYELDYFLYPQVLTMPHPLSTYGADSTEDIDVNLPQILPDIMDLIDEEVD
                     FTQYGGIIIFHSGTGQESDIESIRPNEIWSTFLTRKMLQKYFEPENDLYPGFITNDGA
                     ILTNVIIVPEDEYQDYFPTEGVDDASSYLFSMYGVLAHQFGHILGLPTLYDNDSSNGI
                     SQGIGNWGLMGTGLWNGNGYVPAQLCAWSRYYLGWDNPVIISQNSLNNAVDHFLNHSP
                     TAIHLYKVPISATEYFLVENRQQNPDGSLDPITNSPSYSFKLLPEGEQDYYPDDPSTP
                     QDESLLPYFNFMENSYLGSEWDFFLPGLGGPIPNNSTYLVDGSGILIWHIDENVINAN
                     FTANFDKNRVNAISAHKGVDLEEADGIQNMDTGLNSIYKWGSPYDSFRDDNNNYFGHQ
                     YHNGVLSLPTSESYYGGIPLEICNISASGLQMTFSVIYSWRLNTSYEGTNPINAAGID
                     FDNDGTDELFYPMPDGRIYIWKNEELMDGYPLYGMPIPYTYTWDGENLFIPMQRDRLC
                     RLYKHNKNESKYVYNSPNYSWVGHPVDLGNSIAMPLKSNNEENTFSLQLYSKEDNSVN
                     TIVDLSGNFKANLVYFHNKLSVVYENTSGEYWLSDIDLADNTITDSKLPISADSTIIA
                     IFKAPVKDDENLIVQCQNSVYCLKGANIVDGFPFVHNLSANSDSSFIAPLSIADVDGN
                     GALDILIGGESDYAVIDYAGRLMNPENPSPQPHSDFISAGILAMDIDNDNKAELIGNF
                     KNNRLKIWENDFRDKRGYPVSFAERSRTLPFVAKASDNNWYVYCATDNGSLFRNQLEG
                     EPKFSAAYNWISEYGNLRRTASIEATDLPNQYQTNELFVPGEVYIYPNPLKSIYRQKI
                     LLNVMTNRDVELELSIFDISGTLVYRQKGFAKAYLKNLDIFDIPSAKLCSGVYIAILR
                     SSEGSKRLKFAIEK"
     gene            47476..48378
                     /locus_tag="PLB65_02300"
     CDS             47476..48378
                     /locus_tag="PLB65_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOQ77387.1"
                     /translation="MKQIFTTLCLILISAMCFAGINKDAGTYGYKFLNVPYGPVALSL
                     AGRGVYSSNNSSAFILQPASSCVNDQRLLGITHNMWLADTQANMISYSYSRKIDHFGI
                     AIRNLDYGDIENRDDMGIMIGSYHPIDIDATANYARRLSPNFYTGVNLGVLYQKLNTA
                     SSLALHSDLGFCYLPPVKNTKISLAVRDLGVANKTDDERVKLPTSFEMDINKGFLLAD
                     QQIFLGGSAIQTLDEDLKGSINLEGNLFQIFSIRGGYLLGYDAQDFSAGFGVKYKNIS
                     VDYGYGAFNSELNDVHSFGVTYRF"
     gene            48391..52605
                     /locus_tag="PLB65_02305"
     CDS             48391..52605
                     /locus_tag="PLB65_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOQ77388.1"
                     /translation="MNNNLGFMQNRRVGLVFLLICLGLFLQAEITPNQIIFKTTVPVQ
                     IKGTKTGLNAFDSFLTQQGVKQIKPIKGMHQPNYYLANVSEMPSIQTLQQLSFYGIQY
                     VQPNHISKLHTIPNDPLYNQQFHYICSIPDAWNYTTGSKYIKIGVVDSGVLIHHPDLW
                     QNIAINPNEIPDNGIDDDNNGYIDDWCGWDFTDAPEMAENAIGDYIGQDNDVEDENYH
                     GTHVSGIAGAVGNNGIGISGVCWNVGILPIRAGFRTLDNQGYLQDDDVSAAIIYATDN
                     GCNVINMSWGDPDYSAIIADACEYAYSKGVVLIASAGNDGVSGLSYPAKLSTVISVGS
                     VNNAKQRSTFSSYGVDLDLVAVGENVLSTYKLDPGEQYFRLNGTSMSAAFVSGAAALL
                     LSLHPDLSPAEVRASLLTSTDDIYSVGFDIQTGHGLLNVKKILDNLAPPYLEITSPQD
                     QLGISGSVPIIGSVYGPDFADYTVCYKLDDDANDPYWKDVTTHQMDPITHLEQVHNSE
                     LATFYIPDYFPEGKYLLRLQYEKKINNMNRYNYYRTVYVDRSAPVIRSETVSGFTRWN
                     RQNLQYYVSANFNEKVNASLKITASDGQIYNVYSTLADSIHIFKLPSYIPEGMISVQF
                     VATNITGLTSVSDTFNDLLNIHYCLIPHYGYTKEVIGPPQKPLLRKRDYNGNGVSEYV
                     SMEIPASGYGPVKAFELCSGEHIETNDFGDAFWPLDIGSTYTTGSKSELLLLRVENAE
                     LWLTPLGGNYPSNSNDSLVIALDTGISGGVMADYDNDGKTEILLVKNLPAERVIQAYK
                     RISTGALVQRNTLSNPTTTYSRNNFVPTIIVENLDGDNYPDILCADTDGDIMIYEIIN
                     PTEHHLRWTTRLPVGNTYILTSGDFDGDGNKDFFVAGYNTDITNPNLSFWYGEGFRNT
                     GNNTYSSMGNIMFNEVSTLNSITNADLDKDGCNEIILAITPNLYILKYSNGNFTPVYY
                     DESYRNYQVVTLEDENGAVRIMANIHSDGDNAVSAQWIPSASFTGPATPANLTVKPLN
                     ENSINISWISTGAPEYVLYRKDETGNTVSYNLSAATNYTDTGLQQGKAYQYAIAAIDP
                     SYTPSESNKGPWIEGIPLPKPDVIEINLAGQKELRLLFNQAMPNDIINPGHYFISNGL
                     GNPISANSISGNYGVQLRFRNNFPPIDSLFVIELRNLTGISGVDPVQNFYTFSYQEDV
                     IPPEISQVNVLPSQRGIIITFSEEILPNPALHLANYILHCPQNDPDNTIVSIAHSVDT
                     VTITFAEKLKFTNYAYYLEINNITDLAGNLISPQGNLARFGIQNLNNLDHLNVFPNPV
                     TSKHPQEATFINFPLNKKGKIAIYNSGGQLVFKDDIGPFFEGSNNITYTWNLKNNDQR
                     PVSSGVYFYVVEMGGKLKRGKLAIIK"
     gene            52722..53075
                     /locus_tag="PLB65_02310"
     CDS             52722..53075
                     /locus_tag="PLB65_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HOQ77389.1"
                     /translation="MFWKIIISVFNSPVYTLVISTIFLLLNILDGHSTYLVLKPNYYH
                     REKNPVARWVFKKLSIPNGIIIFKTVLMAILILAISYYTAWDSLTINIAMTVADLLFL
                     IVVIHNYRLYRRIKH"
     gene            complement(53137..54489)
                     /locus_tag="PLB65_02315"
     CDS             complement(53137..54489)
                     /locus_tag="PLB65_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HOQ77390.1"
                     /translation="MIKAEVFRQYDIRGIVNEELDENSFYLIGKGFGTYIRNKNLKTI
                     VLGGDARHSTPSFMQAFSKGALETGCDIIDLGIVPTPVLYFSIWKLKTDGGAMVTASH
                     NPPRYNGCKLNLGLSSVYADELQKVLQIIQKGDFVSGQGNYVKNNDMGDIYIDYVVNG
                     IKLQRPVKVIVDGGNGVGGPYLTAILRRLGCEVIDMFCEPDGDFPNHHPDPTIAKNME
                     ALSKAVIENKYELGIGLDGDADRIGVVDEQGKLLLGDQILNILARDYLKHNPGKKIIA
                     DVKCSMNLFNDIIKYGGEPIMYKTGHANIKMYMQEKKVEFAGEMSGHIFLADRYLGFD
                     DAIYVSCRFCEIVAQTLNPVSSFLADQPKLFNTPELHTSCPDAKKFEVVAKVCEEFKK
                     EGYEVNDIDGARITFSDGWGLIRASNTTPIIVTRYEAITEERMNEIKGLIEGKIQKYL
                     "
     gene            complement(54506..55096)
                     /gene="rdgB"
                     /locus_tag="PLB65_02320"
     CDS             complement(54506..55096)
                     /gene="rdgB"
                     /locus_tag="PLB65_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HOQ77391.1"
                     /translation="MISDILVATHNPDKLKELQELLNNLKVQLHFLDELPQFLPSEEN
                     GKTIYENAMQKALEAAKYSGLLTLADDTGLFIEELNGAPGILSARFAGNSCSYSDNRK
                     KVLNLLQGVENRKAYFETVVALALPDGIVAVLGGKLEGEITTEELGDNGFGYDSIFAV
                     GDKTYAEMDSEQKNKISHRALAMKAIIPILEKVIID"
     gene            complement(55093..55623)
                     /locus_tag="PLB65_02325"
     CDS             complement(55093..55623)
                     /locus_tag="PLB65_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HOQ77392.1"
                     /translation="MKEDDVIIGKEIPVEEQEKIKDKIIDDDVDSTKLQFYEDLRKKV
                     RSWTNTQLGPLGGKLGEYVFLLPDFFILVCRIAVDKRVSVKQKLLMGGVIAYVMMPID
                     LIPDFIPVLGFVDDLVLIVLGLNMILNELNPQIVLDNWSGEGEVLEHLKKITALAEQF
                     LDKHLLNKIKNVLKKI"
     gene            complement(55834..58557)
                     /gene="ppdK"
                     /locus_tag="PLB65_02330"
     CDS             complement(55834..58557)
                     /gene="ppdK"
                     /locus_tag="PLB65_02330"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOQ77393.1"
                     /translation="MNTPKRVYLFGNGKAEGRADMKNLLGGKGANLAEMNLIGVPVPP
                     GFTITTEACIEYNKLGAAKLREILDNDVHLAIKHIEDIMHMQFGSVENPLLVSVRSGA
                     RASMPGMMDTILNLGLNDTTIQGLIKKTNNERFAWDSYRRFVQMYSDVVLGMKPANKE
                     EHDPFEVIINQVKKEKGISSDLELTTEDLKQLVTLFKQEVQEKIHKKFPDDPWEQLWG
                     AIFAVFDSWNNDRAIFYRKLNNIPDEWGTAVTVQAMVFGNMGYTSATGVAFTRDAATG
                     ENLFNGEYLINAQGEDVVAGIRTPQQIAKIGSQRWAKLAGISEEERKEKYPSLEETMP
                     ETYKELYEIQKKLENHYREMQDLEFTIQEGKLWLLQTRTGKRTGAAMVKIAMDMLREG
                     IIDEKTALNRIEPAKLDELLHPVFTKEGLAKAKVITLGLPASPGAATGQIVFFADDAE
                     AWAENNKKVILVREETSPEDLRGMSVAQGILTARGGMTSHAAVVARGMGKCCVAGAGK
                     LHIDYKNRTMTVNGKTYKEGDWISLNGSTGQVLEGKIDTQDPELSGDFGAIMQLADKY
                     TRMKVRTNADTPKDASVARHFGAQGIGLCRTEHMFFEGERINSMREMILAEDEQGRRE
                     ALKKLLPMQRSDFEGIFTAMNGFPVTIRLLDPPLHEFVPQEEEAQQEIAKELGIPLEI
                     VKARVASLHEVNPMLGHRGCRLGNTYPEITEMQSRAIMEAALAVKARGINVLPEIMVP
                     LTSIPAEFEMQETIIRETAEKVFTDMNDRVDYLVGTMIEIPRATLLADKIAEHAEFFS
                     FGTNDLTQMTFGFSRDDAGVFLPIYIEKNLLKQDPFQVLDREGVGQLVKIGVERGRKT
                     RPELKIGICGEHGGEPSSVEFCHMVGMNYVSCSPYRVPIARVAAAKAAISE"
     gene            complement(58948..59583)
                     /locus_tag="PLB65_02335"
     CDS             complement(58948..59583)
                     /locus_tag="PLB65_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDC27 family protein"
                     /protein_id="HOQ77394.1"
                     /translation="MKKIIYIAFGLMLLNLCFAQESPVTKYLQNQNLDTYKQAVDYLV
                     KMDTDGETGMQAKLNLAYISNLESLRLLDLAKENLDNLKPGERFALANLFLEMDKYPE
                     AIEIYDLLNEATPLWSCPWRHKGEALYKSGNFESAKIALGKAIETNEEHYDAYIWYAK
                     TLYELKEYKQALTALETAFNLTEENEESEFDMVLSEEELNQLYQELQKLAK"
     gene            complement(59599..59880)
                     /locus_tag="PLB65_02340"
     CDS             complement(59599..59880)
                     /locus_tag="PLB65_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HOQ77395.1"
                     /translation="MFTINEYFDGKVKSIAFQNKQGNFTVGVMDIGEYEFGTNTIERM
                     TVISGELIVRLPEKENWQKFCKGETFTVAAHQKFHLKVEEPSAYLCQYE"
BASE COUNT        18517 a        11545 c        10398 g        19537 t
ORIGIN      
        1 actattttcg gtgatttcgg tggcctcggt ggctaaatat tcttttaaaa tccttcaggt
       61 atatcttcgc cgggttaaaa cccgtcactc aaatttatca gaacaccttc tataatctat
      121 tttataaatt aaggaatgct tgttttgtat tcgaaaaaag gggaggatat atatcctccc
      181 cataatttat attttcttgt tatagtgttt atcttaatcc ctgagacatt gcttcgccca
      241 gatcaaagat aggcagatag attactacaa taatgcttcc aacgataaca gccattaaga
      301 taattagtag aggttcaatt aaagaagtta aacgatcaat aactgaatca actaatttct
      361 cataaaattc tgcagctttg gaaagtaatt tatccatatc tcctgtttct tcaccagtag
      421 ctatcatttg taataaagta ggggggaaca tacctgttct tctgaaggca tttgctacgc
      481 cgtaaccttc tttaaccaag acccttgctt tacgaacggc actttctatg actgcatttt
      541 gtacaacatt ttctgttaat tccattgtat ccataatggg aactcccgca gccattaata
      601 tactaaaagt acgggaaaat ttactcatta tggaattggt gataacttta ccaattacgg
      661 ggatttttag cttgatgctg tcaaaaacat agcgtccttt atcggttaag ttaatcagga
      721 aaaaggcgag tataattagc agtaccacaa gaatagttac aaatatatta ctagtgatga
      781 aattactgat attgatggct acagtcgtag gagcaggaag atcagcatgg aaatttgcat
      841 aaacatcagc aaacatcgga atgatataat agaacattcc ccaaacgaca aagcacaaga
      901 aaatgatgat gaatactgga tatgctaaag cactgtatac tttgcgacga gtatcctcaa
      961 ccttttccaa gtaatcagct aattcatcta acacggtatg gagagtacca gaaacctctc
     1021 cggctgttac taatgctata tacagaggat taaaaactcc cgggtgctgt tgcattgctt
     1081 ccgaaagtga atatccttta cggatatcgt cggaaagttt acgcaacacc ttggcaaatt
     1141 ttttgttttt ttcttccttc tccaaatcag atatcgcttt ttcgatagta agtccggctg
     1201 aaaacattgt ggagagttga cgagtaaaga acaccaaggt ttttaagctg acaccggttc
     1261 tccatttata aatagcgagc ataactttat cgaaaaggga gagttttcct ttaaaagcac
     1321 cttcagcagc tgctttaaca ctgataattg tgctcccttc cttagaaagt ttatcaactg
     1381 cgatatcgag agtatcggct ttgatcattc cctcgattct ggctcctttg gcatccttaa
     1441 taatgtacgc gaaatttggc atagcggttc accttcctaa aaatatttat tcgattacag
     1501 tcattttgat gacttctcgg attgtagtga tcccgttctt catttttgtt aaagcggcat
     1561 catgcaaagt acgcattccg ttatctattg ctgcttgtct aattaaatct tgattgccac
     1621 cttcataaat aatactgcgg atttcaggat taatagttaa gagctcaaat ataccttctc
     1681 gtccagaata acctgtatta tcacaatgca cacaaccagc cccaatcttg aaattgattt
     1741 tggatgcttc ttcgggagtt aaacctgcat ccattatttc ctgctctgtg gggatgtagt
     1801 cctttacgca atatttgcat attttacgaa ctaagcgttg agccataacc aaagataagg
     1861 aagatccaac tagatagggt ttaataccaa tatctatcat acgagtaatt gtggcaggag
     1921 catcattagc atgtaaggtt gtaaaaacga ggtgaccagt taacgaaaat ttgatagcta
     1981 tatcagctgt ctcttcatca cgaatttctc caatcaaaac aatatcaggg tcctgacgta
     2041 acacagaacg aagtgcagaa ccgaaagtaa gtccgatttg ctctttagtc tcaatttgag
     2101 taacaccttc caaacggtat tcaacggggt cttcaactgt aattatatta gattcaatat
     2161 cttgtatttc tttgagggca gcgtgaagag atgtggattt tccacttccg gtgggtcctg
     2221 aaacaataat aattccataa ggtcgtctga taacctttct aaagatatcc atatctgcag
     2281 attcaaaacc gagagtggtt agtttgaatc caaattctcc cttatccaaa agacgcatca
     2341 cgactttttc acctaaaact gtaggtgtaa tagaaacacg aacatcaaca ctttttaacg
     2401 atgtctttaa agcgatatga ccatcctgag gtaatctgcg ttcggcaatg ttcaatttac
     2461 tcattacttt taccagagaa attaaactgg catgcatacc gatgggagga gtcataactt
     2521 ctcttaaagc tccatcaaca cgaaatctta ttcgcgaaac tttaagtaag ggctcaatgt
     2581 gtatatcggt ggctccagct ttaatagctt cgttaataat aagattaatc aattttacta
     2641 caggagcatc tacatcttca gtggccgctc cgatagtgat ttcattttcg tcttcatcta
     2701 ctgctacaaa ttcaaaacca cctactgcat cttcaacctc tgtagtggtg cggatgcttt
     2761 tatagtattt ttctattgca tcgtgtaaat ttgaatcagc aatcaaaacg ggcttaatat
     2821 tcttgcctat tagttttttc aagctgtcta aaacattcag gttttcagga tcagccatag
     2881 ctactacaac gccatcacct tcttctctta aagccaaaac tctattttct acagcgtatg
     2941 gctctggaat caaacttact atgtctatat caagatccat tagttctcgg tcttgaacta
     3001 catcatagcc aagttgttga tgtaaagcgg taatgagttc attttcggtt agatatccaa
     3061 gcttgataag cgtctgacca agtttcaaac cgaaattact ttgcttgacc aatgcctcct
     3121 ttacttgatc ttctgttaca tatgcctcgt gaactaagat ttctcccagc cgggcaaatt
     3181 gtggattata aaccattact atttccttat ttgaaattat ccatattgat tttggtcagc
     3241 aggtcggatc attgaggcat ataggctctg aaagatccaa cccaccaacc aaatctagca
     3301 tttatttttt taccaaggtg cttgaccagg gaataagatt aaggtaacag ttacttcccg
     3361 gttcagcaac ccatccatca atgttcctct aattctcatt ggagtaggtt gtggaattcc
     3421 ttcgatagga ggaatacact cacctgcttt gaaagcaacc cttccctttg caaaaccagt
     3481 ttcatcagtc aataacatcc accactcgtt tggaggtgaa ctggggtcgt atacaccatt
     3541 tggattttga atacaatgac ctccatccaa aggtagcaac ataacctggg catccttaac
     3601 aggacatccc tgaccatctg ttaatgcaaa gtaagtgtct gtatattcat aactggtttg
     3661 gttattatca aacatcaata ctgctggttg tatttccata taagcttcag gtgcattcag
     3721 tggtaaaata caatcagtat caccaaaaat tggagtacca taagggtcat caccacaatt
     3781 agctctgatt gtgattgttt gatatgttaa agtaccatca taattgatat aagtgtacgc
     3841 aataccttct gttgaatcac catcagcatt tgtattacct acataagatt gggcaatgat
     3901 ttcgcagcca ggattattaa tgatagagaa ccaaacagaa gtttgataat caacagggtt
     3961 gttatacata tcttttacaa ttgcaccaca ttgtacacgc cataaaccac cgcctaaatt
     4021 gtcaccacta tcaaatccgc tgacaaagga aacaattcct cctggtactg gaggacctga
     4081 atgaataaca atgttggttt tcaaggcata gatatagcga gtgcaatcac tatttaccca
     4141 agatgcttgt atcttcacaa ttcctgaatc tgttccacta ttaattgaga cttgtgcctc
     4201 tcctccattt gaaataacct ccacagaatc ctgttgagga taaccattca agtttgcccc
     4261 ttgaggtgca ttcgtatttg ctattttgaa atagaccttc tgaggaacat cgattaagtt
     4321 gccatttata tctctaagct taactcgtaa tattgcagat tgtaatccac ctgtattggc
     4381 aacatttaag ttgatttgtc cctgctgagt aaaatccaag gaatataatt cactggaagt
     4441 taccataaat attgtttggg caaataatgt atccccgttt gcagatgctt taattgatgc
     4501 tgcacctgca gaaagaccag gagtaaatct tacagaagca acgccatcag tttgagtatt
     4561 ctgagttacc gtctgagtag tatttaaaaa tgaccccaaa tctgtagtaa attttacggg
     4621 ctgataagag catgcattgt tgtaaatatc agtcaggcct gctttcacaa atatccaatt
     4681 agggctatta accgaacttg tatctgcttc aattagttga tcatttaccc gtacatatgt
     4741 cttcaaatct atattagcag gacgtccagg tttgattatt atttcttttg tatcacttgc
     4801 attaccaacc tttgctgtaa caaaggcatt atcagctcct ggcatagtag tggcatcagt
     4861 ccaagaattg tatctaactg tagctctccc gttaaaggta gccacaacta cactggttcc
     4921 tatttcatta tcttggctat ctacaaagcg tcctttagta cattcaaaag ttaccaaagt
     4981 gttattaggt acttgctgat catcctgatt tattggaata gcaattacat taatagtttg
     5041 cattacagac ataagatagg gatcatttaa actcgggagt tttatgttta ctgattttat
     5101 cggaggaatg ggtttgattt ctacatttat agcagtagaa tcctgccaaa ctaaagtatc
     5161 gccactggca tcgtaatttc taactattgc cgatatagtg gctgttcctt gatcaccatc
     5221 atcccaaaag gttgttctgg ctattccgct gctatcagta tggacatcag ttaaaactct
     5281 gcctaaatta gttttaaatt tcaccatttg attgaatacc ccaaaacctt ctccatcttt
     5341 cacttccaca gaaatgatag aataggtgat attataatca gcataaattg tatcaggatt
     5401 agcagttatt cttgttatat atctttcctg ggaagaagat acaggtactt gtgttggagg
     5461 taacacaggt ggtggattgc gtttgtcaca gcttgcactg aaaccaatta ggatgatcat
     5521 taatagcgta aggattgaca ttagtgaact atgattcttc attcttgttc ctcctctccc
     5581 tcttcttcat attgaataag agtgcgggtt aaacgcttgt caacttttac tgtcggactt
     5641 gtttcctgca tagagacaag ccggggagtg atttctatga tcaggtcagt attttctacc
     5701 agttcataac gatgctggaa tattttaccc actaagggaa gatcgcctaa taatggtact
     5761 ttattaactt tttgagttat atttgaattc aataaaccac caacaataat cttatgttcg
     5821 ttaggaacag tgacagcaga agaaactcta cgaacttttg ttcttggtac atatccacca
     5881 accaattccg ttacagaact cacttcggga ttgatattca tagtgatatt gccatcttta
     5941 ttcactttgg gggtaacatt taaaataata cctgtttctg aacgttccac ctgaagttgg
     6001 ttttccttgt cagtaactac gaagggaact acttctccaa tatgcatagt agccagctct
     6061 ccatttaggg ctgtaacacg agtatcggta agtaattgag cagcattatt ttctaacaac
     6121 caatcgatag taacatcaaa agcagttaat tggcggctaa aatggccaat atcgttaaaa
     6181 ccatcaatac gctggaaata ttgttcatca ggaacaactc caatatcagt tggattacca
     6241 tgtggtaagt aaccagttgc atcagaatag ttataaggta aacccgttcc atattggttt
     6301 acaggatcct ctgccagaat tgtggtaaga tgattcaatt tactccagtc aactccaatt
     6361 tggcgagctt cgtttagatg aacttcaatt aggcgaactc taatttctac ctgttgttgt
     6421 tgagaatcaa tagattttac taactcttca acttctctga aagtatctgt attgccgtag
     6481 atcattaaag cattctgacc ttccagagct gtaattttgg cagtagtatt ttgaagagca
     6541 tcactaactt tcttagcgtc cagattgttt aaataaatta tattgctgcg ctcacccatt
     6601 tcagccataa tatcttgttt ctggccaaca aggaaagtat tattactaac tactcgataa
     6661 gagagaccag cagcactgga aaccaaagaa acagctgttt caattggtac atctttaatg
     6721 ttgatagtaa ccctcttttc ttctttctct gtagttcctg cttgatctac agccagaaca
     6781 atattggtgt tgctcaatct cgaaagagct ttaaatactg atgaaagggt ggcatcatca
     6841 gcgttaaaag taacttttgt atccaatatg gggtctcttc ttggttgtgc atttaagaac
     6901 gatatgcatg ccagaaaaga cagcataagg ataatcgtat aacgacgttt gcaatgtcta
     6961 atgtgtttca tatttactcc cagtatcatt tatattaatt accaattccc gcttgttccg
     7021 ctaccataag ttgttgtttc ctcaactggt ttagctggac gaggggcgag ttcagtagta
     7081 gttattattc cattatgact gtagcggatg ctcttatctt ggatatccaa aatacggcgt
     7141 ccttcaacga tatcacctac gcgtgcttca tatagcttac cttgatattc aatataggca
     7201 attttattgc caaactcatc gcgagcggta gtagctaagc gaaaagtatt tttcagcatt
     7261 tcttggtatt ctttttcccg atctattttg tccttaatga tatttccttg tcgcaagggg
     7321 tctttgttag aattaaatac aaacatttta cgatcttgga tggaattttc aatggacttt
     7381 attctgctta gaagagtatc ggaaacagat tcttttgtat gaaccgattt atctggaatt
     7441 gcttcccagc ttctatataa tccaaccagt tgaactgcca tagcaaatag tgagatcacg
     7501 attagagcta ttgtaaggtc tttaataaat ctcagttgca tcttatgcct ccctcttcac
     7561 tttaaacaca gaaaattcaa gggtaactct gtaacggttg ggagcattgg cagcagtaac
     7621 ttctttatca gaagcttgaa gaggactaat atccagggcg tgaattttca ggatgttatc
     7681 catcgattcc aaatctgaaa taaactgccc aatctgaaca taggtagctt ctaattccag
     7741 gttaaaagta gtttctacta aacctgggaa ggagaattta ttactatcgg agagtttgtt
     7801 tatagtaatt gttcgttgat ctgccatttg accaattttg gttttgaatg cgttgatttc
     7861 gtcaaaacta aaactagctt ccctggttaa tgaattatct attatcaggg caaactgact
     7921 gagttgttga tccataatgc gggcgctgtt taattgttcc tgagcggttt tgattttctg
     7981 atccagttct ttaatcttat tgcgattggt ggatacacta ttaatagtaa acacaaagaa
     8041 cagtactgta atcagcaata tgcacaggac gaagatcaaa tacttttctc tcatttcacg
     8101 cctccttcag ctacttccct actataacat tcaataacaa attcgagaat agctgtgtcc
     8161 tttacaacct gtctagtaat agatccggat ttaattcggg ggaaatcatt gctgatttct
     8221 tcattagctt tcaaacgatc gatgaaagat tgaataagat ctaactccct aatattctta
     8281 ttaacttcag taataccatt tagagttaag attccattgg cataagaaaa tttacgaact
     8341 gccaattttt catcgattgc attacttaag gcaaccattt tctttgccca gaatattcta
     8401 ttattgaatg tttctgcaag cttatcgaga tcattactgg aaagataatc tttactggtt
     8461 tcatagcttg ccagttctct ttgtagttca ttcaagtatt tttggcggct ttcaactctt
     8521 gcctttgcca taccattaat ataaaaccaa gaggctaaaa taataataag cccaacggca
     8581 aagacaacta ttgaatttat gaagaccttt ttctcatgtt ctgcctgcag gcgcatttca
     8641 ccatatttat ttaggttaat tttgaagtaa aacatatttt ccatcagaaa actccttacc
     8701 tattccaatc tcattgctag gccgatagcc aaagcaagtt gtggatcttt tttatcctgg
     8761 aatttttcag gcatttccac attgataaaa ggcataaaga tctctgttgg aatagctact
     8821 ttctcttcga taaattcttt taaacctttc agtttggcgg ttccgcccat aagatatacc
     8881 ttgcggaaat cactgtttcc tgcttctttt acatagaagc gtaaggagcg gcggacttct
     8941 tcagcaatgg attcttctgt tgatttctca gtaatatcaa gcatactaac agtttgtatg
     9001 ttttccacag cagggttttc gcctaaaccg aattccagtt tatggtgttc agcttcattc
     9061 cattccagtt gacgtttacg cataatatca cgagtaaaat tgtatccgcc gtatggaata
     9121 tcacgagcaa agaattttgc ttctggaccc cagataacca tatttgttcg gtgagcaccc
     9181 agatttagta atacataaac tccatcttca acaaaagtat ttaagataaa gctattggct
     9241 attgctaaag aatcgatatc tacaatatta ggcgataaac caacagtcat caaaacattg
     9301 gtatgctcgt ttaatagatc tttggaggta gcagccagca gtatattcat attgttggtt
     9361 ttctcttcca aattgataac ctggtaatct aaaatcatat ctgatccgct gataggaata
     9421 tgttttttgg cttcaaaaaa cagggcagat tccagttctt catcaggaag gaagattgtt
     9481 ttgatctgtt tgatactggt gttatcgcct ccaatggatg taaccagatg cttaatatgc
     9541 ttaggattta tcttaagcgt tttcagcatt tcgtttagca cgggaccata acgctctgga
     9601 cctaagtcac ttggaatata ttccgttcca gttggaatcg taggacttat ttcatagttt
     9661 aaaagcttaa atccttcgtg cagtttcttt aagtgaacaa ttttaatact gtgggttcca
     9721 atatctattc ccacagtttc tttaaatcga acttgctttt tcttcttcat tcttcctctt
     9781 tatttatcat attatatcac atagtattct atattaaagt gcgaggtggt ttcttcaaat
     9841 accagctacc ctgttctaag ggaagacgtt ccccctgctt tttaaaataa ggccagaagg
     9901 gtggcttcac tgtatacatc cgtgtatcat agttgtagtt ctttttataa cctacacccg
     9961 agcctgaagc tccaggataa tgacgactct gtaaagtaac accaacagca ggattttgat
    10021 atagttgaat attagtagct gtagtgccac aaggtccacc gcataaatct gtaggcggat
    10081 tccaaacacc actgtttgat gggtattcac tatcattcat acttctatga acaaaaccac
    10141 gccggcgttg atttactcct ccccaaatat taattgttcc tctttcaagg tagggacgag
    10201 cttccggcca aataggatta taccaaggaa aatctagcca agcaggccat gaatgtgcgg
    10261 ctgattgagg ccaatagttt cggtgaatat caatccaatc aaatacgatc atccctgcat
    10321 cgggatcatc agtcggagga ttaaatttca cggctggaat tgagccatga ggatgttgat
    10381 attcaaaagt aaatacacca tctccatatc gatcatttac tccttttcca agggcagcca
    10441 tagcggcata aatccatata ccattatcta aatctccatc cgaacccatg ttagtatgaa
    10501 ctcgtaagga atcagaagga tcacgatagg catattttat aataactgat tgttccgaaa
    10561 ccaagccaag catatctttc ttattctgta aaggtgaagc acctaatgct gtatccgtat
    10621 atgttaaatc tccaataagc cacatagtat cagcggaagc ccaagtttgg aagcctttaa
    10681 atgttccttt tatccagagt ttgcaatttg cccaataagc acccccatta tgggcagtca
    10741 tactgaaagg agtccagagt gtatctgttg tcggaaagta attaacagta gttgggggat
    10801 cagtataagg atagcccatt ggataattag gatatagttc cttgtgttct cttcttgttg
    10861 taatctgacc ttgccaacct gatacttgtg tgtcatctac cgttaccatt aatattctgt
    10921 cttcaccgct tggccctatc tgggatccaa aagctcgaat ttcattcata ttagttggaa
    10981 attcatattc atcataattt tcgattaatc ctccttgaaa aacattatcg cgaggatagc
    11041 ttggcggaga agatataact acgccagagg tggttacgat agataaaaaa gtaggccaac
    11101 cattattatt tccgccaccc aattgtttta tctgaatatc agtattacaa tgaacaggac
    11161 cgttgatcac atcataccca tagaaataca catttgtatc attaggtgag agctcactat
    11221 cggtaaaata cataaataca gggcctgtat cttgcaataa agttaattcg ccatatttac
    11281 gagtgaggga taaatctctt ctgccataat aaatatcgct tgttccggtt cttccttcaa
    11341 ccaatgaacg aacttgataa aaagtaatac ttgctccagt tggagtggct tgtaattcac
    11401 cgccaacttc tacataatct gcatcagttt ccttataaat tttatttata agtgactgca
    11461 tagaatagag tttacggtaa ttacttccat tcatctgaat ttttctggta ggagttctta
    11521 ctcctccaac aatatcagga tctctttttg ctgctacaga tagatatgct tgacctctat
    11581 ctgcttctgt tctcaaaaag tgaacactct gaatgtcttc caattcagac atagcagcta
    11641 cgctattgcg tagggccaaa ctggacatag ttaatccact catagcacct ataactgcca
    11701 agagcattgc gatagcaata ttgccgcttt gacttttaag gatgtttttc ataaatcaac
    11761 ctcctgaatt tcatggagat ggtctatcca tatttttcat tgccataata gtatgaaaat
    11821 ggacttttct atactcatct tttctaattt taactttggc tttaaggtct acattaataa
    11881 cgcacatttt ctcatccggc ttataggcaa aaatggcatt attcgcattt gcgtcactaa
    11941 ttaagaagtc ctcaattatc actctgtcct taaggccggg ttctggaaat atgacctttg
    12001 cagaagggga atctgttccc cgatgtctgt aatccatttt gattactcca tcgtgaagat
    12061 aataccttac aaaatcattt ggatatttat ctgaaattgg aggttttatt tcgactccgt
    12121 gactaacacc tggaacggaa ctcatccgaa taagagtaat ttcttttgca ctggttattc
    12181 cttcaaattc tatatctgaa gaacgagaac cgatgtgatg gccatatttc agaacattta
    12241 aaaaggctac catatctttc tgtaaatcta cataagcatt cagctcttga tatttacgaa
    12301 aaaagacacc tattcccata gaggctgtta ctattaatat ggatgagatc gtaataacag
    12361 ctatggtttc aaataatgta aatccctttt cattgaatat gatatgtttc atggctcgtc
    12421 ctaatcgtaa aaatcttcgc gcaaacgcac aaaatgtact ttttctgtaa cgggatcaac
    12481 ccaacggatt tctccaatta catattgata accatatgta tgagtcatat agttctcaac
    12541 ttcccttccc acggatatag acatcattcc agtgacggtc aattcttctg ttttgtcaat
    12601 ttctacagga acttcagtaa acggtcggaa aatattttcc ttcatatata gggtatattg
    12661 tttttccagc tcacccgtta gtagcaaagt agcaacgcgc tgtctataat cctgagtgat
    12721 ctttttttcg gcaaactcta tacctatata tacagtaagg accatcaccg ccaaaacaat
    12781 ggcgactacg agaacttcag ccaaactata ccctccctga gaaccaagaa cgctaaaatt
    12841 aaagcttctt tcatttggcg aattgttctc aggtagggta tgaatatttt tcttcattcc
    12901 taaaaacctg ctcctgtagc aaattgttta ggattatctg caaattgttc cgctattgtt
    12961 ctttctatca gattattcat caccaagtta tacaaagatt gatctctggt ttgcatacct
    13021 tcttttgtac ttgcttgaat aacagaaggt atctgatagg tcttttcttc acgaatgaga
    13081 tttcgaacag ctgcattagc tatcataatc tcaacggaag gaacacgacc ctttccatcc
    13141 tttgtcggca aaagggtttg agcaataact gcttccagtg attcggaaag cattgaacga
    13201 atctgttgtt gttgctcttt agggaacata tcaatgatac ggtcaatcga ttttgtacaa
    13261 cttccagtat gcaaagttgc aaacaccaag tgaccagttt ctgcagctgt caacgctaag
    13321 gacactgttt ccagatcgcg catttcacca accagaataa catcaggatc ttcacgcagg
    13381 gcactacgca aggcagcagt aaagctccaa gtatcatgac caagctcacg ttggttgatt
    13441 aagctatttt tactgcgatg tacaaattcg atagggtctt caactgtaat aatatgacag
    13501 ttacgacctt cgtttatgct atcaatcatt gtagctaaag tagtagattt tccgctacct
    13561 gtaggtcctg taaccaggat cagtcctttt tctttggtag ttaaatgtct taaaatatct
    13621 ggaaggtgta attcctcata acttttaatt tcattgggga ttacacgaaa ggcacaagat
    13681 acaccattta tctggtgaaa tgcatttact ctgaatcgga catcgttact cagcttagtt
    13741 gagaagtcaa tctccaaatt ctttttaaat agtgcctgtt gagattcatt cataactccg
    13801 aaaacaaggg tttctacttc ttccggactt agaataggta aatttagttt tttcatacga
    13861 ccatttacgc gcaccatagg atgagcgcca gcagctatat gaaggtcaga agctccagct
    13921 tctgcagtaa agcgtaataa ttcatggatt gtcaagttac tatcctcctc ggggttaatc
    13981 ggtttccatt cctccagttt caggattggt ccaagtatct attccgtaac catggaattg
    14041 ggcatctgta acatcatacc aaacttgttt tccttcacca ccggcaaaat cttgagtaga
    14101 agttgcaata tattttttag gtgggttacc gacaacttca aatttccaat ttttaacggt
    14161 gctttcaccg aggttggctt ctttaagggc ttgttcaacg ctaaagccat ctgttgaacc
    14221 attagtttgc atatagatat cataagtctt acgaatggtg tttattgcag tttgggcttc
    14281 tgtactacga gatttttcta cataacgaag ataacgggga acggctaagg ccgccagaat
    14341 ggctacaata ataaccacca ctaagatttc gattagggta aaaccctttt ggtttttcag
    14401 tttcctgagc atttttttat cctcctacag gaatgttttt tttactttta tatatatgca
    14461 caatgcgtgc caaatcccaa ttaaaaattt aaataattga aaatagggag agtgtaaaca
    14521 gctatagcac aagaaaaagc gttgtttggt tatatagttg ggcgaatgtt gaaaaccgag
    14581 ataagcaaat tatgggcaac ctgaaaaagt aaaaaagaac cgcagatatt taaactataa
    14641 atgctttttc aaaatcgtat ataccttata gtataaaaca acacttgact tcttttacta
    14701 tcttacaact ttgtctcaca gacaaaaata atttattaag gagcatacga tgtcccgatc
    14761 tttaagtttc taccttgggt tgctcttact gtgtatcaat attacttttt taggagcaga
    14821 taatacccct aaacctgtta gcaatgataa gcgaataaat gaaacggata ttttagcgga
    14881 atttgatggc ggagtaataa cccgtaaaga tgttgaagct aagatttcca aacttcctgt
    14941 tcaataccaa gcgcgttata aaacagttga aggtcagacg gaagttttaa atgctatggc
    15001 tatggaagaa gttttttata aaaaagctca acaaatggga ctggaaaatg atcctattgt
    15061 tttggagcga atagccaata ttgaaaagcg tttttacatc caggaatatt ataaaaggaa
    15121 tgtaagtgat ttagtagtta ttacagaaga cgatctacaa tcctattata atcaaaatat
    15181 gtcccaattt tatcttaatc cctatattga aatagattat cttcaagctg ctgatgagaa
    15241 tgaagcaaaa aaagcattgg aagaattaaa aaaaggtgtt tcatttgctg aggtctccga
    15301 taaatataac caaaatactt atgccaaggg tttgaaaggt agaataaaaa acattcgttt
    15361 aaatggaaat attccaggta ttggcaacga ttacgagtta gaaaatctta ttcgtacttc
    15421 taaagtagat tctctatctt ttattggacc agtgaacact tcttacggat ggcatatttt
    15481 ccgtactgtt aattgggtag acgggcgtca aaaagatttt tctgaagtaa agcaagaaat
    15541 cgaacagcgg ctgcgtcctc tgaaagaaag agaattattg gattctatca tagagaattc
    15601 taagatcaag tatgcagttg ttatcgatag ctctttagta aacacaatca ctcttgatgt
    15661 taataaacgg aataataata accctattct ggataatgta gttcttagtt ccagtgatcc
    15721 agatttacga ttcaccgtga ggcagttatt acaaatttac gataagctat cacctcagga
    15781 acaacaattc gttatcaagg gtggtggaat taacctgtta ataaatcaag agctcactca
    15841 atatttgatg tatctggaag ctaaggcagc tggctatgat aaatatattg ctgataatga
    15901 agattacttg caagcaaaac gtgcctatat cttacgcaga gcttatgaaa aacttattac
    15961 cgagaatatt gaaatcagcg atgaagaaat tgctcaacgc tatgaacggg atagagaaca
    16021 gttttcctct cccgaatatc gcactattga agtcctcttc tttaaagata aaaaaacagc
    16081 tgaaaaagca ctgaaaaaat acagcaaagc ctataagaaa aataacgaaa aagcaatgca
    16141 agatgtaatt aagaaatatt caatcaaacc gaataacgct attatggatt atcaatatag
    16201 aaatggaatc ataactggtc tgggcgatga tgcaaaattc tctaatatga tttgggataa
    16261 tcccgttgga tatttgagcc ccgtttttac tactgcaaaa ggggatattg ttttcttcag
    16321 gacaattaaa gaaactccca aagtttataa gagtaaattg gaagtagaac ctcgcatcta
    16381 tggaatgctg aaagccgaaa aagaaaaagc aaagcaagca gaagttactg aaaacctcta
    16441 tgtggaattt aatatgaaaa aatatcctga acgtatcact cttactatga cagccgaaga
    16501 actttttacc caagcagata atgctgctag acaacgaaac tatagagacg ctattgttta
    16561 ttacgaccaa attattaatc actataaaaa taatgttgat gactataaag catcttttat
    16621 gaaagcattc ctggttgctg aagaaatgaa acaaacagat ctggctttac aacttttccg
    16681 tgaattcttg caaaaatatc ctacaggcga tctaaatgaa tctgctcaat ttatgattga
    16741 ttccttggaa ggaaaaattc cggaacactt tgaacctgtg gaagattaat aataaatgta
    16801 cataaaacgc tggctttaaa attctaagct ggcgagaata taataagcag ctatatatat
    16861 aaggaagaat ggcataccgt cattcttcct ttttctattc aacaatccta ataatgctgc
    16921 ccacagccct aaacataaat atagtcgata aatcgctatt ccttctttat tacttgcgct
    16981 gtagttgtag tgctgatgtt gggaattttg cccagtatca gcacggcagc cctaccgtag
    17041 atactaagga agaaataagc tggaattata agtaaaagaa tgattgcaga aataactaac
    17101 agatggtaat caggatccaa taacagtttt tacagcttct ttttttaagc gccctagttg
    17161 gccattaggt aaatgaacca gataatggtt attttcgctt tgcactattt taacgatcaa
    17221 tcccgaatgg actgtttgga taacattatt acttgtgggg gagagaaggt tagtttgatt
    17281 taaaatcaca acagcattag gattatgttt ttggtggtgc agttttattc ccagagttat
    17341 tgaagaagct atgcagataa ttaaacacag agctaaaact aagattggca gagctctttc
    17401 cttttctgga tcataaaaaa gcagccaaca tagggctaag gcagatagaa acagaaagat
    17461 aagaacaaag atagcaagcc tgttaacgga aaaaaaatcc agaactttat aaagaacctg
    17521 caccaaaaaa aggtgttcag gatataaatc tgcatctgga ctatagcgga tagataggtt
    17581 taagttgttt tttgcctgag aatgggcaga attgatgttc aatgctttta agaagtataa
    17641 attagccatt ccgatatctc ccctggcaaa gaaatctact cccagattat aaaagagatc
    17701 agcattcttt ttttgttctg cgtctcggat tgctttatcc agggaatccg gtaattgagt
    17761 aagctcgccg gaatttatat attgtgatgt ttttaatgaa tctgtttgag caaagagagc
    17821 ttctcctaaa ataaagcaga agatacataa gaaaattaaa cgccacttca ttttgtgctc
    17881 ctgttatgtt tatttttacc caaacgagta aagccgttaa atatcttacg aatgcggttt
    17941 agatcttcgt ccaaatttac ggctctttcc gattctggca taaatctggc tgcttggcta
    18001 tggtttaaaa atagctgtat ttcttccaga agccaatctg ggatattttc ttggctaagc
    18061 gcctgcaatt tttcttttgt ggatagacgg ttggagagct tatatttgga agcaagatat
    18121 tcaaacagag ctttttctgc caaaggatag aattccagcg acaaattttt ggcagctaaa
    18181 gtggctggtt tcaaataacg aagcaaaact ttttcagctt tcaaagaagc atagcgttct
    18241 ggattatctc ttttcatttt actatccaga gcaaaaaatg caacaaataa agaaaaagca
    18301 aagataagta ataccaaaat ccaataccag ggctgttgga cataagggac ataaggtgga
    18361 aaatgctttt tcactacttt gggcagcatc gctttgggtg ttttagattg tcttaaacgg
    18421 ttaaagtaac ttaatacatt tgccgaggaa acctttatca tttcttttgg gctgcggtag
    18481 gttttgtagc taccgctata gttatcaaac caagtaaaac taagttctgg aagttcataa
    18541 cttcctttat cctgaggaat tagggtataa taataggtac gctctccttc aattcctgcc
    18601 ttgagattgt ctataaccat tggagtagat acctgaaatc ctttcccaga agcaaattca
    18661 ggagcagaga attgattgaa attacctctg cccttaattt ttagggtgaa agtgatagtt
    18721 tcccccaaag aaagctcttt acgggaaaga gaatagctaa ttttaaaatc tcctactgct
    18781 cccccaaaag aagctggaac attgtttttg ggcaagggca gaacatttat aataccccct
    18841 tcactgcgta gggttttatt ggaataactg aaacggtata aacgagcata accttctaaa
    18901 ataggagctt gcaaccttcc ttctttattg ggaataattg ccaatctttt aattagagcc
    18961 cgcttatact gctttccgtt caaatttaca ttctcataat tcaaaacaga gggttgctca
    19021 taagtgcttt ttccatagcc atcggaatca atctcatttt ccaaattgaa tgagcgaacc
    19081 atttcatccg tatagagata gtaagaaaca atagtgggaa aaccacgata aattgtttgt
    19141 tcttgtggaa ttgctaaaag aaaagaatct cctatccact gttctctatc atcccaagaa
    19201 tctggttcat caaaaccgaa agctccaaaa ttgggatatg aaggtgcact ttgacttggt
    19261 gcttttgatg ttcccttaac tacttcaata ttgatttctc gagtagaata aaagcgtcca
    19321 ttcactttca cttgaaaaga aggaatagtg gtttttccag tttttaaggg aaaataaaaa
    19381 aaacgaaagg tttctgaaaa ctcggaaacc agctttgttc cttcaaaaac tacagaacta
    19441 ctggagccac ttgtcatatt gcgaaaacta aatagaggaa tagaaggagg agttggttca
    19501 acaactttaa ttctatcgct gtctgtaatc tttaaggtta cttccagttg atcatttgta
    19561 ctgatcacat atttatttac tgataaatct atcttcaaag cagatagcat aagcgggaaa
    19621 agcataatca acaatatatt ttttttcatt tcaccaccat ttatcggttt tgaaactttg
    19681 tttgggcttt tgctgctgac ggtctgtgct ttctttatta tccaatcctt ttagaatatt
    19741 aaggatatct tcatctttat gcttatcttc attctgttgc atcaatggct gaggatttgg
    19801 ctgtttttgt aatttgcgta aagtcaattc ataatttgcc cgtagatcat catcattagg
    19861 attcaaaagt aaagcttcct tatagtcctt tagtgctttt tgataatcct tttgtagata
    19921 ggcaatattg cctctatcgt agagaatatc ttttttatcc tgggcattgt tcaaagctat
    19981 ggatatggca gaatcggctt cggcaaaacg gttttgctta tataaactct tggctaaatt
    20041 ggcataagct gcagcgtcat tatttgtagt gtttttacgg aacaggtttt ctgctttgtt
    20101 atatttttgg gcttggtaaa aagaatcagc aaagctctgc cgaatcactt ttggccttaa
    20161 tagtaattcc agaataagca tcaaaattat aattactatc actaataaaa ttatccgctt
    20221 tgctttaatg ctcatcacta tgctccgcag ccatcaaacc cctttttctt tttctgggat
    20281 caaggaggga ttctataata agtaaaagca aagccaaaat gccaaaaagg taatattgct
    20341 ctttcaatat agtaactttc tttcctcggc gggtgctttc gctctcatat attcttttta
    20401 atatcagctg gatttcttct cctccaggag ttacccgaaa atactctcca ttcgtaagtt
    20461 cagcaatctc ctgaagggtt gtctcatcca gaaaagtttt tacctcttct cccgtttcgg
    20521 gatgacggat tattgttcct ttcggacttc ccactcccat agtgtaaact ctaattcctt
    20581 tggccttaag ttctttggct ttacggagca cagaattttc aagatcttct ccatccgaaa
    20641 ttaagattaa agtattagct ttagaatctg tcggaaatgc ctctgcagcc aaagcaagag
    20701 cttttccgat attggttcct ggaatttcta cactattact gttcaaaccg tttaaaacaa
    20761 ttcttactgc ttcgtaatca tcagttaagg gacattgtaa agtagctgtt cccgcaaaag
    20821 caataatgcc aattcggtct gtttttacct gatctaaaaa actaccaatc tgtaaaatag
    20881 cacgcaacaa tctactgggc atcatatctg tggcatccat acttttagaa acatctatag
    20941 caaatataat gtccattccg ctactttgca aatccttttt ttcataatcc cattgtggtc
    21001 tggtgagagc tatgattata catcccaaag caagaatgca gataaataat ttaaaaccta
    21061 tccagaaagg tgattgagaa tgataataat gctcttttaa atgcagctct gcgtactttt
    21121 gaaagcgacg ccgtaatctg ttttcccttc tgaaaagtaa aataagaaag ataaggcaaa
    21181 atgccaaaag tgctaaataa tatggatttg caatattcat agctggtctg gtaaaatagg
    21241 taagataacc gttttcagta acagttcaat agccagaaag atgaaagcaa gccataaaaa
    21301 aggcataaac tgctcactgt agttatatct gaattgggtg gtgaattggg ttttttccaa
    21361 gcggtctatt ttattcataa tatccgctag acttttagca tcagttgcta aagctgcttt
    21421 tccagttcct gttgttgttg ctattttatt cagggtctcc atatccaaat ctattagtgt
    21481 attaatatag cgagttccaa aaacaggatc agaatagggg aaaggaacta atcctttact
    21541 tccaactcct atgggataaa cttttatgcc aagttcctta gccatttccg ctgcagttaa
    21601 agggtctatt tctccagtgt tattaacacc atctgtaata agaatgatca ctctactttt
    21661 ggctgtgcta ttttttaaac gggcaactgc ttttgccaaa cccattccta aggcagttgc
    21721 agaagcttct tcattaactt ttagcttttc taaagaattt aacatagcaa gatgatcaaa
    21781 agtaagagga acctgagtta atgcatattc ggaaaaagct actaagccaa atctgtcgtt
    21841 gggacgcttt tttacaaagt ccttagctac actaactgca gcacttaatc tattttgagg
    21901 ggcaaaatcc atagctaaca tagaaccact gatatcaatt gccattataa tatccacacc
    21961 cttattactt aaatctcggg aacgagttcc ccaacgcggt tcagctatag cgatacaaag
    22021 aaaaaatatt attaaggcac gtaaaacagg gaaaagataa cgataaagat attgattgtg
    22081 agataaaaat cgcaactgct ttactctgga atgaggtaga taattccttc ttctattgtt
    22141 gaactttaac tcccatacta aatataaagg aataatcaac aaaagcaaca acaaccaggg
    22201 atggccaaat ttaatcaatt ctttgcctct attgtgttac caataaaact aatcaaatag
    22261 tccttcagcc ataaagtgta attctcaatc tcttgtagcg agggttggcg tttggcaaat
    22321 ttaaccatat cacaatagtt tataaatgct ctaatttccc gataattcgg aatattgcgt
    22381 ttgtttagat aagtgatgat ttcgttacct gtcatttcca aggcgggaaa atgataggtc
    22441 tcttctaaaa agccacgcaa aatattggat aaccgaaaat gatataaaac cacctctcct
    22501 ttggcaagta aatcttcttt aataagctca tctaacgctt gtatagattt tttccaagca
    22561 ggtaaaatag cttgtttagt ttctggctgc gttatatact ctcgcttagg ttttttagga
    22621 cgcttcaacc aaagataaat tgctaaagct attgcgatta ataataaaat cagatacacc
    22681 catattccta attgtaaagg atacttttgc aaaggtttaa tatcccttaa aagagtatcc
    22741 ccttcggcta aaactgaaag aacatacact ctaaaaccat cagtgtaaac aggagttaca
    22801 gagctgtcaa ctggatttac tgctatgcgt ggaaaagata atgcccctat ttttagaggt
    22861 gctattttaa tttgccagct gcgggtattc ctttcatcct gtctgctttc caatatagca
    22921 aaatcctcta aagtatcagg gatagaaaca ctatgtattg ggtaatcggc agttattttt
    22981 aagaggaaag gagttccaac ttttaagctg tctgctcctt gaagttcttg atggaatgcc
    23041 caaattatca aagggaaaaa gataagggag aaagccaaaa cgctggattt tttcatcgtc
    23101 tttttcctct tagagcaaag aattttcgta atgaacttac ataagaatct gaagttttga
    23161 tcattagata atcacaatta cattcgcgga aaaattcctg caactctttc tgttcattat
    23221 ttattacttc ttgataggcc ttacgcaatt tgggatcgga actatttatc caaatttctt
    23281 ctccggtttc gggatccttc aaggataaaa tgcctgcagg gggaagttct agttcgctat
    23341 catctacaac ctgtaaagca acaacttcat ttttagcagc caggattttt aaggagcgtt
    23401 catatccaga atcgatccaa tctgataaaa taaaaataat acagcgtttt ttcaacattt
    23461 tataggcata ttcacaagct gcttgcaggg atgtcttttt cccctttggc tcgtaataga
    23521 gaacttcgcg aagaatttcc aaagcattat ttcttccttt tcgaggaggc aaaaaacgct
    23581 ctacttggtc agaaaaaagg atcaggccag ctaaatcctg attggccact gcagaaaaag
    23641 ataaagctgc cactaattca gcaatgcgtt ctctttttat catactacgg gtgccaaatt
    23701 cttgtgaact acttacatca accaagaaaa ctactctcaa ttctcgggtc tcacggtatt
    23761 tctttatgta aagctgaccc aaacgcgctg aaacattcca gtcaatatct ctgtaattat
    23821 ctccgggctg atattcccgc acttccgaaa attccaaacc ctgtccttta aaccgactgt
    23881 gatattctcc acgaaacatt tcggcaacgg gatttcgcgt ccgaatttct atcctgcgaa
    23941 ttttttttag gatatcggaa acactttggg taaacataag cagagatata tctttaaggg
    24001 acttctattt catccaaaat tcgattaatt atatcttcgg tagtaatttc ttcagcttct
    24061 gcttcataag aaagaattat ccgatgcctt aaaacatctc tggcaattgc tttgatgtca
    24121 tctggaataa cataagccct accagatata taagcattgg cttttgctgc tcttgccaga
    24181 taaattgtag cgcggggtga agccccaaat tcaattaatc cctttaaatc gtttaagcga
    24241 ggataacgtt ctggatgacg agtagaatta atcaagtgta aaatataatc cttcagcttt
    24301 tcttccatat ataccatata catcacttcg cgcatacttt ctatatcagc tggacttaat
    24361 acttttttca gaggaattgg ttgttcattt accatccttt ccaaaattat tcgttcttca
    24421 tcgtaagaag gatatttgat cttcagtttc ataaaaaatc tatctacttg agcttctgga
    24481 agaggataag ttccttcctg ttcaattgga ttctgagtgg ccatcacaaa aaacggttta
    24541 ggcagtataa aagtgttatc tcctaaagta acttgccttt cctgcattgc ttctaaaagt
    24601 gcagattgta ctttggaagg agcacgattg atttcatcag ccaagataaa attggcaaag
    24661 acgggaccct tcttcacact gaaatcgcca cttttgggat tgtaaatcat tgtgcctgtt
    24721 atatctgcag gtaacaaatc aggtgtaaac tgaatcctgg aataagaggc atcaaaaact
    24781 gaagctaagg aagatattat cagggtcttg gctaagccag gcacaccctc aataagaata
    24841 tgaccattgg caagaattcc aatcagcaga cggtctataa tttcttgctg accaactatc
    24901 actcgggcaa tttccaagcg gacattttca agggctactg aactctgttc tactttgttc
    24961 tgaattgcct caattaacat aaatccccca tattttattc acttacttta agtaaaaaat
    25021 cgggaatctg accaaaccaa gattcccgaa aaatgttctt ctattttatt gagtccagga
    25081 gtaaaaaacc caaacagaac ggattattgt taaattactt tacgaacttt atttgctttc
    25141 agacaagaac tacaaacact tattcgtttg gtcccttttt catcttcaac acgaacttta
    25201 tgcagattag gataaaatct gcgcttagtg gcatttaatg catggctgcg ataattgcct
    25261 acctgagctg tttttccaca tatatcacaa actttagaca taattcactt cccaatttgt
    25321 tttcttttaa accacaaaaa ttattactcc atttttgaca agcaattatt tagcactctg
    25381 tggaatacga atgtcaaaac ggctgccatg tggctctata ggaatatatt ctaaacgacc
    25441 cttatgctcc tgaaccaact tcttgcaaat tgccaatcct aaaccggtac cataattttt
    25501 cccatcggat acaaatggct gaaaaaggtc ttcctgcact ttttgtggta tacctggacc
    25561 attatctata accgatattt gtaaccaccc agaagaaata ctactcaaaa tcttaatttc
    25621 cccttcaaca ggcattgctt caatagcatt tttaataaga ttaattagca ctctacggat
    25681 ttttccttca tcaaaatgaa cataattcgg aactctgttt tctacaataa aatctatttt
    25741 tcgctcttta agattgggaa aatacatttc ctgaagttcc cggaaaaaga catctaaatc
    25801 tacttgttga atcagtggag gtgtttctgt tcctttagca aaatccaata tttcgtgaac
    25861 taattgatca atcagcttgg tttgtttaat aatattctcc gtaaattctc taccctctgg
    25921 aaaaacattc tccaaaacct gcgcagttaa aactataacc gttaagggag ttttgatgtc
    25981 atgaatgatt ttacttgcgg ctaaaccaat agccattaag cgattcttct tgatcatttc
    26041 atccacgagt tctacaaaac gttcattcgt actgcgcaac ctccctgaaa ttgtcctcac
    26101 cagattcaac ataattaatg gataagcaaa gatcatatta tgaaatgctt ctcgtgggat
    26161 gatcatcaat tctacatctt ctaaagctac tacacttgca gagcgggggc tatcttcaat
    26221 aatgcccatt tcgccaaaaa aatcgccagc ttgtaaaata gaaagctgtg caaaaggggt
    26281 ttcgggatta tgtaaacctt tatttatttc taccttccct ttacagatga aaaaaaggtt
    26341 atcgccccaa gagtgttcgg taataattgc ttcacctttt ttaaattgta ttctttctaa
    26401 atctttagta agctcttgca atacatcttc agaaagaccg ctaaaaaaac ccatattact
    26461 acacttcgtg tttttttgca tttttacctc acatctgcta tagttgttaa attacttcct
    26521 aattttaacc acaattgttt tacatcttaa taaaaaggac taacgcttag acaaagaaag
    26581 aaatcagttc cacccagaaa ataaacttct ttcggcaggg atttatctat ggtctgttcc
    26641 atatcccaat ctgtatcgta ataagcatta cctgcaagcc aagaattaac tttgtccttc
    26701 ataaaccatc taccttcaac tttgggcgat tgcctaactc ccaaagccaa agctaaaaac
    26761 caattattat atattctaac tcctaaacga gctgatgctt cagcggaaaa atgtagatag
    26821 ggctctggct gattaatttt cgttacccga tatgtataat tggctgcatc atattcacac
    26881 tcaattgatt ccacaaataa gtgttccacc cctaaggcag taattttatc agcataggga
    26941 gcatcataag tagctgctgt ctttattcct gcatttagat atgcattata attcagcaag
    27001 ggataggtgt aactcccgga taacgataag acatccataa aatatgagtt ttttaggtca
    27061 ccattaaaag aaccttgaat tgcagcaaca ttatctaaaa tcccaaaagt aaccataggt
    27121 aagggtttgg gaacagtttg agaggaaaat ttggagtagg tataatgtaa tataactaca
    27181 gaagctgatt ctatatgttc aaaaccctca tccggattca gatactgata gtagtttcca
    27241 gaagttaaat atcctcctcg catataccgg acaaacgact cttgaggtgt aaccggaact
    27301 gtttgaggaa caaaaaaatc tattatctga gccaattgtg cattaacggt tccgcacaga
    27361 gtaaataata taacaggcag cagaaaacga atatgtttca cgataaaact ctctttcctt
    27421 attgatttaa ttatttcaat aatgccaata tctgtcaaga ataattattt atttacttca
    27481 tttgtgaaat ggagtaagct ataaagtctc actgaaaaaa agcctgcaca actatgcgtt
    27541 ctaatacaat gttcaatact ccggctggtt attgaatgca gatggatatg tgtaatcttc
    27601 gcgtctaata ttatgttaac tttaccattg cgggcggttt tcttccttca ataacggaaa
    27661 tagcattttc tacagccaga agagccattt tagttctggt ttcaatactg gctgatccaa
    27721 tatgagggag taaaactaca ttatccaagg ctaaaagttc cggaggaata taaggttctt
    27781 gttcataaac atccaatcca gcggcaaata ttttttttgt ggaaagtgcc ctaatcagtt
    27841 cttgttcatc tataatagga ccgcgagaag tatttattag aactgcgcta tctttcataa
    27901 tttccaattc attttttccg attagatgat aggtattttc cgttaggggg atatgtagtg
    27961 tgaggatatc tgcttctttt agtagagtgt taaaatctac ttcagttgct tcatagggca
    28021 aattttggtt aagaggaata tcattatgat aaataatctg catattaaaa ccgacagcgc
    28081 gtttagcaac tgcctgaccg attctaccca tacctaaaat tcccaaggtt ttgttattaa
    28141 catcctgacc taacatcagc attggttccc aacccttaaa tttgccactt cggataaagc
    28201 gttcactttc tgtgattctt ctacaggttg ccataattag agcccaagta agatcagcag
    28261 ttgtttctgt taaaactcca ggagtattgc aaaccacgat tccacatttt attgcatatt
    28321 caatatcgat attattgtag ccgactgcat aattggaaat aactttcaat ttaggagctg
    28381 cgtctataat ttctttatct ataatatcgg ttagtaagca gatcaaggcg tcggcatctt
    28441 taatgttttc cataatttcc tgtttagtta aagagcggtt atagggattg atctttaaag
    28501 taaaattatc ttccagaagc tttattgcag gttccggaag caaacgagtt aaaaataaaa
    28561 gcggtttcat agatacctca caaaatcctt cacttgatgt ctttcaggaa caggaaaatg
    28621 ttcagcagga tagccaattg gagttaagca agcaatataa tcttgatgcg gaattcccag
    28681 caaatcctta atttcatctt tatccatatc ggttacccaa caggttccca aatcataagc
    28741 tttagcagcc agcattaaat gataagcagc aatacaggca tcctgaatct tccgatgaga
    28801 aatatctcct ttagaggcaa cggcaatagc ataaggagaa gctaaaattg gttttccgga
    28861 aggacccctt aaagaaacaa ttttctccag aatttctcgc tgcttaatta ctatataata
    28921 ataactttgc aaattacaag cagtgggaga atagcggcaa agttcaaaaa ctttatttaa
    28981 aagctcttct ggaaccggtt cagatttata tttacggata ctgcgtctgg ttttcaaacc
    29041 ttcttccaat gttatatatg gttccaattg atgatcaaaa cactgaaact ctaaagatct
    29101 accttcagga tcggcagccc agaaattata gatattatag gcaagattag gtttcggagc
    29161 agttttggct attgctttta gcctcgaata gatagcatca acttcttttc tgtccttaca
    29221 gaaaaaagtt attgttccag agatttcact tatatcacat ctacaaaaac caagtaattg
    29281 atttccagct ctaaaaatta tgcaagaatc ctgattaagc cacacaggga gtgccaaaac
    29341 tgaagtgtaa aaatccacta tcaaatctaa cttttttgtt ttgaagaaat taatagcatt
    29401 catcataaat ccttaataag ttactatagt ataatgtaat ctattttacc cagaaaagca
    29461 aatataaagt aggatggaca caagatttaa tggacttagt tttagataac ggactacagg
    29521 attataggat tgagcggatt tcttttaaac aataagaaaa aaaagaaaag aggattatgt
    29581 aaatccggag taatttgtgc caagacaact acaatgtttg aactaagaaa tccctgcact
    29641 tagaacttta aacttaaatc ttagccctta ttctttgttt agtatcttct gcattcatag
    29701 ccacccttat ctgttctccg gtttgtaaat tttttagatt tatagtttta ttagcaagtt
    29761 cgtcttctcc gataataatg gcatattttg ctccgctggc atcagccgct ttaaattgtg
    29821 ctttcataga atttttatcg ggatcataat ctacaataag tcccttaatc cttagatcat
    29881 ttaagtaagt taaaacctct aagcgtacat tttctcctaa acaaacaagg taggcatccg
    29941 gtttcggctt ttctgcataa ttaatctttt cttcttccaa agctataagc aggcgttcaa
    30001 aaccaccagc aaaaccaatt gcgggaacac ttttacctcc gatttgttca ataagtccgt
    30061 tatatcttcc tcccccggcc aaggaatttt gtgcgcccaa accttcgtaa actatttcaa
    30121 aggcagtatt tgtatagtaa tcgagtccgc gtacaattcg aggattgatt atatatttta
    30181 tcttcattgc ctgcagatag ttacatacag cctcaaaatg tttttcacag ggctcatcca
    30241 gataatttaa ttgagaagga gcatcgtctc taagttctat acaggaaggc actttacaat
    30301 caagtaatcg gcgaggatta gtgttaaaac gattttggca atcggggcaa agtttaccta
    30361 aaaaaggtct ataatattcc tgcaaagctt tatcgtaaag ggcagcacaa tcagggcaac
    30421 ccacactgtt aatttccaac cgtaatttat tcaatcccaa gctattaagc catagcatag
    30481 aaatggcaat tacttctgca tcgtaatagg gattgttact accgataaat tctatcccat
    30541 attgataaaa ttgacgatat ctaccagctt ggggacggtc gtaacgaaac ataggcccta
    30601 aataaaaaag ttttgagcgc ttggataatt tatccaaatt attttctaca tagctacgca
    30661 ccactggggc tgttccttca ggacgcaatg caaattcgcg acccttttta tcgcagaaac
    30721 gatacatttc tttttgaact acatctgagc tttccccaga actgcgttca aagagttctg
    30781 aaacttcaaa aatgggggta gtaatttctt gatagccaaa acagatggca aatttacgaa
    30841 agctatcctg caggaattgc catttatagc tatcttcagg cagaatatca aaagttccac
    30901 gagggatatt atatttcatt agtatttcct cttaaatttt taaattccat tttgttatta
    30961 tcttctcttc agcatatata ttatattata gtccttaccc ttttagggca aatcggaaat
    31021 ttcaccatta ttttcctgta attcgggaag gtaagattcg tcaagaaact tatccggata
    31081 tttcacattc attaagtaaa gccctgaggc aggggctgtt atcactaaat tctggcgggg
    31141 acatttcttt tccaaaatat ccgaaatcgt ttcaggagga agattaaaat gggaaatatt
    31201 agccagagtt ccaaccattc tccgaaccat attatgcaga aaacggtctg ctacaaaatc
    31261 aaagctaaaa tagtctgttt gctcgttaat atcgcatact ttcaattcac acattctatt
    31321 gggtatttct ggattgttgc gtccgaaaga agaaaaatca tattttccta taagataggt
    31381 agctgctgct tgcattcttt ccagttttaa agttaaatag ggtataaaac cagtataatt
    31441 acggttaaag ggattttttt ccttagctaa aatataacga taacttcttt catacgcttg
    31501 atagcgagca cttaatgcag ggcttacttt ctttatttcc aacactttaa tatcatcagg
    31561 aagatatcgg ttgaaagcta aaagcaactg atattctttc attgaaccca tataatcaaa
    31621 atgcgcatat tggcctaatg aatgaactcc ggcatctgtt cttccagcag caattaaaga
    31681 atttttttca caggtaaaga tttgtaatgc gttttccatt acttcttgaa tagcaagtcc
    31741 gtttttctgt ttttgccagc cgcaataagc tgtgccatca taacatatct ttattaagta
    31801 tcttgccatt tttcctctgt attacaaact gtgcactata actaataacg ccaggaagaa
    31861 tattcccaga tagttagccc ggtagttatg gatttctttt tcgcgctcaa aacttaataa
    31921 atcagtaact gatgtgttta tttcaggcga aagggaagat acatttttta gaacatccct
    31981 aatctgagct aaaaccaagt ttccaccttt aacctgtttt agatttctat attcagcaaa
    32041 aaatgcttcg ataaaataaa tggtagcaaa cagataataa aacaaatagt ttaccgtttt
    32101 ccacctgcgt aacctcttgc tctctatggc aatacccttt agtttaacat ttcccagaac
    32161 ataaactgta actgcaatca aatatagtat ttgtatactg aagaacacag ttgttaaaaa
    32221 atcttgagca aaaagagttg caaaaaccca ataaccagca aaaaaaggca ataatctttt
    32281 tagagaattt aaaaataacc gatataaaga aggttcaaaa cctaaaataa ccaatatcaa
    32341 aatcagctga atcaatagct ggcgaagcga agaatctact cctattgcca gacctgttaa
    32401 aaaaacagct aacttcaaat agagatgctg tcccgaaatt ggataagata tgaacttcat
    32461 tcaaggggta tttcttcttt tttttctgga actgactctt ctggaagagt aggaacagga
    32521 ttttcttctg gttccggagt atttacttcc ggagtttcag tttcaagtga actaatatct
    32581 tcttcctgtt tgttttctac cggaggaata atcttagcca aagtatcaac taaaacagga
    32641 atttcattat atatttttaa actatccgac aaggatttat gtatctctgc tccctccgga
    32701 agaggtagtt ctttcttgtt ttcgttaata tttaaagaag tggaatctac tacagtagaa
    32761 tcagcaacag gaatatcaaa tttaggagct cctcttagca agaaaatatc accattaaag
    32821 aatctttgtg tgatagtggc atattcgcca gcctcaggtt cttctaaaac agcatttaaa
    32881 taaggtttag ccaataaggt atcgacctgt tcaaaactgt aaaaccatcc caaacgataa
    32941 ttagcggcca gcttaatttt tggatagcgc gaacgggtaa gttcttcaag acctaccaaa
    33001 gccgaatctg gagcagtgta tatttgttcc agatacagct ctaaaagctg ttcttctcgt
    33061 tcttctaaag gatcaattaa acgcactggc agatcattct gtaaggcata tagcgctttt
    33121 gtaaatttat tctctggata ttgttcttgc atttgggtta aaatttctgc attttcagga
    33181 ctttctggat agttgttatg ataaatagag cctatagcaa acaaacaaaa aggaattatt
    33241 tcactatcaa accgctctat aatctcttcc agagtattta ttctgtcttg acataactcg
    33301 ttttcctgtt ccaatctggt aatttcagaa cgaattgata caactaaagt atctatctgg
    33361 ttagaagagc tatcctgtat tgcaataatg ttatccttta gtaaactatc ttcagcagct
    33421 atttccggtt tcgttatttt taaggaatca gcagtgtttg ataaactatc tgtaaaagtg
    33481 ctaacatcaa gaagatcatc taattgggta agcaattcag ttttatctac tatacctata
    33541 ctatcttctt cagaaatttc ctgtatcggt acaatggctc ttaacgaatc cagaattgca
    33601 ttatttttac tgataagagc tagtaaagaa tcacatctgt ttaccagaat atctttttga
    33661 gcaataacaa ttctatagcg tgctatggca gaatctggta aagctaatac attcaaaaag
    33721 ctttctgcag cctgatattg ataatccaaa aaattgctta gatttttttc acaatcaaga
    33781 ccttggcggg gagagaccaa acctaaggct gaagatttta cctgagcaga tgctgcaaga
    33841 ggagatgaag aaaattcagt tcgtactcta ttataactag caattgcttt atgaatatct
    33901 ccttgattgt aataatagtg ttctgccaag taataatatc cacctgcaga acctgctgtg
    33961 cgaggataat ttttgttgat aagttcaatt tcagatctgg ctgcagttgt atcccccaaa
    34021 gcaaattgag ctcttgcttt caataaacgg acgaagggta atttatcagg acgagattct
    34081 gcttttaaca atccttttgc agttttgagt gctttttccg cttctcccaa ttccaattca
    34141 tttaatccga tataataagt tccatccaat ttttttgctt tatcgattcc acgagcgttt
    34201 tgcatttttt taaatgcttc cagggatttg gcaaagtcct tttgttcata gtagttcttt
    34261 ccaaaaagga aatatgcctc gcgatattct tttgttttag gaaattcgtt aattatcctt
    34321 tccagatagt gttgagcttt aatataatct ttatcctgta tagcaaaatc agctaaaacg
    34381 gatagagcca ttggatgatg tttacggtac ttagggttgc gtaaaaattc atcgagtagt
    34441 ttttcagcct ctttgggttg atttgtttct cttaaaattt tggctatgta gatatatgct
    34501 tcgggaacat atttactatc gggaaagcga attaccagat tttggaattg gtcttttgcc
    34561 tggaaagcgc tattaccttt atagtaaagg gattttgcca tcaaatagta tgcttcttca
    34621 acccttttgc cttttttatc agtagcaata atttttccgc atttctttat cgcttttgta
    34681 tattcatcaa ttgcttgagc atttggtcta ccattactgt ttagcggttt ggattgtgcc
    34741 gatttaaaat aatttctggc attatacata gtatttacct cacaggcaaa aagcccgata
    34801 aggcaaagca gacagataat tattactcgg catttcataa ttcaataacc gcggaatgct
    34861 atattcctgt caattaattt cagagcaaat ttcatccaaa cgcttagaac gaacaagttc
    34921 aattgcttta tttacatccg gatacattat cctatcacca tctaaaggag caacattttc
    34981 tcttaagctt gccttaactt tttctaaaac aggcgagcta caaatttgtc tttgatccag
    35041 tgccgaacag ccaattagta gttcaattcc caaaacagaa gccacatttt gcactatctg
    35101 gcaaagtttt atagctgaaa ttgaacccat agacacatga tcttcttgat tggcagatgt
    35161 aggaatagaa tcaacacagg caggatgggc tagaacttta ttttccgaaa ccaacgctgc
    35221 agccgtcaat tgctgaatca taaaaccaga atcaattcca ggatgcgttg ctaaaaatgg
    35281 tgaaagccct ctggatagag aagaatttaa catctgttca attctgcgtt cagatatgga
    35341 agcaagctca caaaccgcca tagctaaagt atctaaagca atagctaagg gctcaccatg
    35401 aaaatttccc cctgaaataa ctttcccctc atcaggaaaa atcaacggat tatccgttgc
    35461 cgaatttatt tccacttcca aaacactacg cacataagca caggcatctc taacggcacc
    35521 attaacttgt ggagtgcatc gtaagctata agcatcttgc acattcccac aatttaaatg
    35581 ggattcccgc aaagggctac cctgtaaaag agttctgata ttttttgcgg atgttttctg
    35641 accggcatga ggccttacat tatgaatcag ttcatcaaat gctttgggtg ttccttttaa
    35701 ggcatctatg ctaattgcag caataatatc agcataacga cataaacgtt ctgcttccag
    35761 aagatttaga actcccaaag cagccataac ttgagtccca ttatttaagg ccaaaccttc
    35821 tttggcagca agttttacag gtgttatact tgctttttgc attgccacac ttcctggcaa
    35881 acgctcacct ttataaattg cttccccttc accaatcagc actaaagcca aatgtgaaag
    35941 aggagaaaga tctccactgg ctccaactga accccgcatc ggaattatag gatgaattcc
    36001 tttattcaac atctgtacca aggtttccaa agtctctaat cgaatgccag aatgaccttt
    36061 ggctaagaca gcaatcctta aaagcattat tgcccgcgtt tgcacttcag aatatggttc
    36121 tcccacgctg gttgaatgac tgcgaattag atttaattgc aattcagcta tgttttcctt
    36181 aggaataatt acggtgctga atttaccaaa accagtattc aaaccgtaga caatttcacc
    36241 actttctatt attttatcca cataagcacg acatttaatc actttttcct tgctggattc
    36301 cgacaggcga atcggagcat tataaagagc aacctgctcc actgcttcca gcgttaaagt
    36361 attgccgtca ataataattt cctgcatttt atcctctata tatagtaatt cttatgatgg
    36421 taaacctaaa aaatgataat tttactcctt tatttctata gatatgattt tgtcaagcca
    36481 agaaagctct cacattggtt atattgtatt ttatcctttc tgccttgctt gattctctaa
    36541 cctcaatcct cattatctcc ttctccactc atagttcagg atgggtttag gaggagagaa
    36601 tgaggagttg atgaggagtg gttaacaagc aagatgtaag taaccacttg agaaataaag
    36661 tggtagcaag aaaaaacaat tacacttaat tgaagggatt cttgctttcg cagaattaca
    36721 gaaacgattg cagaaagatt tttaccaaat tggaaatcgg taaactatct tcaaattccc
    36781 tcttatcata ttataaggta acaatcagct gctgcggact aacctaaagc caacatactg
    36841 ataaccgcga ttcggatctc ctttttctct ccagaggatg tttaaatttc tgccttctcc
    36901 atttaaaaca cttcccccgc ggataacttt ataggttccg gtatctggtc ctgtaggatt
    36961 tataaaagta ggcaaagaac gaatataatt ggaatcatac caatcccaaa cccattcgga
    37021 aacatttccg gtcatatcat ataagccata atcattgggt tttttggtcc catgagtatg
    37081 aattttgcca ccgctattat ctctattcca agatatctca tcaggatctt ctgaaccact
    37141 gtaattataa agttgatctg ctttggctgc taattcccat tccgcttcgg ttggtagacg
    37201 gtatccatta gctttaaaat tgcatgtcac aactctggaa gcacctactc ctcttatttt
    37261 gtaagccggg gttaaacctt cagcttcact tctaccatta caataaatag ctatatcata
    37321 ccaacttaca ttatccacag gtaaattatc tccaaaagtt gaacaatttg ccggtttcat
    37381 atagaggttc cattctgctt gggttacttc atatctggcc atataaaaag aagatagaga
    37441 aacattgtga ttcaggttat ctttcaatct tcccaaacct aaggaattgg attcaataaa
    37501 taccatatta tgtggaggtc ttggtttttg aactacgggt gaaggagtag aaggagtgat
    37561 attggtaaca ggaagctgct ttctaaaatc ttctcgagca gattttgttc ctgattttaa
    37621 ttcaccataa gatgtttgca aaggtgcatt gcgttctaat tcttttgctg atataggatt
    37681 atttacacta tccgattcgg aatttggggc tagccaggca gaataagtta atttttctcc
    37741 cttacctcca aaaataacgc tgaaaatatt ggtggtaagc aacataacaa ttataccaat
    37801 cattagaata attaaaaccc agaaagtaac ctcaattttg gtttttggga tttctttttt
    37861 gctgatgtcg tccatatcct cagggagtgt tttttcctgt tcttcccaga tttcgcattt
    37921 ttctactgga ggtaaattga taatggcatc gagaaattca ttcaatccaa tatatcgttg
    37981 cataatattg cgatgcaaac attcagataa aattttatta aggggaacac tcaccccgta
    38041 tatgtactca aatttctgat tagctaaccg ttcttgagag tatattgtgg tataaaacgg
    38101 acttttagaa agtacttgag ctaacaaacc agctatagaa aacagttctt ctctttcgtc
    38161 tctttcttcc caagtgattg cagaagaaag aattactatc tcattattat cttttaccag
    38221 aacattagtt aaggctaatt tacttaaagt taactgatat tgatgaagtt ttatggcagc
    38281 ttcaactatc tgaatagcta taagacgaac aaaatcctca gtcaattttt ccggattatg
    38341 cgtttttagc ttcgctaagg agaccccgtg aatatattca acaatcatat agggagggtc
    38401 gtaatgagaa tttacttcta ctactttggt aatgtgttca tcattcaagt aatttagctg
    38461 ttgtaaaagt ttctgcagag aaaatatttt ggttacattg cttaaacttg ctttgtagaa
    38521 gagctttagt aaatattctt taccatcctt ttcagctatg tattttatac cttctgcatc
    38581 tttatttaat aaacggacta ttttatagtc ccgaaaactg ttgccaggat ttaagagaag
    38641 ttgagaacgc tttatatcac ctttatttac ttgctgtttc gaatcttttt tatctggttc
    38701 cttttgctga gttttttccg cttcttgaat ttgattttct ccacagacag gacaaaactt
    38761 cgcttgcgct ggtatagagt ttccacaatt aatacagaat ttattgatga ctgcctccta
    38821 accctattta tttaatttag taatgcttaa atatatctta tgcccattta tatctatgcc
    38881 ttgcatatcc ggtagagggt tctttaatat agttatttct ttagccaggg tttcgcttaa
    38941 tatataatct tgatgggctt gaattgccct agcgatttct ggagtaacta caattttgat
    39001 ctcaatatta tccataatat ccagattcat ttctttgcga gtaaactgaa ttttatttac
    39061 tatttctcga gcaaaacctt cgtttataag ttcatcagtt agcttagtat ctaacgctac
    39121 aaaaagatct ttggcattgg caaaaacata tccttcacgg gggataatgc taacagatat
    39181 ttcttctgga aataaattaa tcgtttcttg ctcaatatcc atagcaaaat aaccggtttt
    39241 gttaaaggcc attaaaactt ctgctgcagg tgttttattt agaagattat taatgatctg
    39301 cactttcttg ccgtatttag gacccaaaat tttgaaatcg ggcttcaatt catactgcac
    39361 aaagccgtcc tgttcgctta caaaattgat tttacggata ttaacttcct cttttacgag
    39421 atccaacata tccagtaaat cttcttttat cttagctgga acaaacattt cacttaaagg
    39481 ttggcgaact ttaatctggc attcatttct ggctgctcta cctaaagaaa caacatcaat
    39541 cacactctgc attttctttt ccagttgtgt gtctatatac ttattatccg aaacaggata
    39601 caattccaga tgaatactct cgcctgcttc cagattcaaa aatagttctt ctgccagata
    39661 aggagcaaag ggggcaatta gtttagctgt ttctacaaga acttgatata aagttcgata
    39721 ggcatctatc ttgtcctggt caaatcctga agcccaaaaa cggcgtctac ttcttcgaac
    39781 ataccaattg gaaagttcgt caataacaaa actctgaata gctctcacag ctttggtaaa
    39841 atcgtaacta tcaaaatagt tgcgaacagt tactataaga gaatgtaagc gagaaactat
    39901 ccatctatct atttctactt cgcgttccca attataagga tattcagaag caatgaaacc
    39961 atcaatattg gcataagtag caaagaaaga atatacattt ttaagcgttc caataaactt
    40021 tcccaaaatt tctttaacgc catctacatc aaaacgcgta gggacccaag gcggagaaac
    40081 ttctaaaaga taccaacgaa ttgcatctgc cccataattt tgcataagtt caattgggtc
    40141 aacggcattt ccatgagatt tactcatttt cctgccattt ttatccaaaa tcatatcatt
    40201 tactaaacag cttttataac tagaaacacc ttttaacaag gtagaaatgg ccagcataga
    40261 ataaaaccag ccacgagttt gatctatccc ttcagaaata atatctgcag ggaatagttc
    40321 cggctcgaaa ttatcataat tttcgaaagg ataatgccat tgagcaaacg gcatagaacc
    40381 actatcaaac caacaatcaa taacttctgg agtgcgatac attttatttc cacaggaaca
    40441 agtaagaaca acttcatcaa tataggggcg atgtaaatcc aaatcttcag gaatatcctc
    40501 tccggagatt aatttcccct cttttcgtaa ctgttcaata gatccaatgg aacgctgttt
    40561 tccacatttt tcacaaatcc agatatttag tggcgtcccc caaaaacgat ctctggaaag
    40621 agcccagtcc acattatttt ccaaccagtc gccaaaacgc ttttctccta caaaaggagg
    40681 ataccagtca attttacggt tgttttcaat tagctgttct ttgaattctg tggttctgat
    40741 ataccagctt tcgcgtgcat aataaattaa agggcttttg catctccagc aaaaaggata
    40801 gctgtgtttt atctgctctc tacggaataa attgccactt tcttttaagt gacggataat
    40861 atctttatcg gcatctttaa cgaatttatg agcccaaggt gtaatggctt ctatgaattt
    40921 accttcgcca tctactggct gaagaaaagg tagatcatat ttcatagcta aagaataatc
    40981 atcctgacca aaagctggag ctgtatgaac aattccggtt ccttcactca ttgttacata
    41041 atcccctaaa cagacatacc aagcaggttt atcaactgtt acaaaatcga aaagaggttg
    41101 atatcgaaga ccttccagtt cgcggccttt catttctgaa attatttgag cttcttcatc
    41161 cagaacttga agacgcgatt tggccaaaat taaattctga tcgtgataat taactttcac
    41221 ataggtctct tccggatgaa ccgctaaagc tacattggag ataagagtcc agggagtagt
    41281 tgtccaagcc aagaaatatg tattatcttc ttccaatgct ttaaacttta catatataga
    41341 aggatcttcc acatctttat aaccctgagc tacttcatga gaagataaag gagttccaca
    41401 acttgggcaa tagggaacta ttttataggc tttataaata agattgcgag agaaaaagtt
    41461 atttaaaatc caccagacgg attctatata actattattt agagtaatat atggatgatc
    41521 aaggtctatc caataaccca ttaattctgt catttcccgc caaagctttt catagctgaa
    41581 aactgattcg cggcattttt tacagaaatt ttcaattcca tattcttcga tgccccgttt
    41641 atcttgaaga ccaagggttt tttcaacttc aatttcaaca ggcagaccat gggtatccca
    41701 tcctgctttt cttttaactt gaaagccgtt cattgtttta tagcggcaga taacatcttt
    41761 caaagttctg gccattacat gatgaattcc aggtttacca tttgcggtag ggggtccctc
    41821 gtaaaagata aaacgagggc tgccttcgcg gatatctatg cttttttgta ctaaattatg
    41881 gtttttccag tattcgcgaa tacggttctc taaatgctta gggctttctt ttagatcgat
    41941 agttttatac atatacctta tccgattttc ttctgatgac ctgtccttct ttgtccggga
    42001 acaaatttag gaggattaga aggttgactt ttaagatgtt cacgttcctt ttgcaactga
    42061 agattatctt gttctgtttc tatcggtttt tcttcaaaga gttcactgaa atgctctttc
    42121 aagtcttcat tttcggaaac ataattcttc cattcttcca tagctttatc tatatctaca
    42181 catttgggac agaggactag taaattttgt tcgctaactc caatccaacc acgatcgtag
    42241 caatggtcac aacttttctt gtgacgattt ctttgggcaa tttcttttgc cgcttctaag
    42301 tgtttttctt cgagtataaa cataatttct ccttacttgg aacatagagg tataaaaaca
    42361 cctcaataaa tttcagcatt gtgagccatt tattttaagc gcacaattct gtaaaaggaa
    42421 atttttgttc ctaaaattac tattctgctt ttttagcttt tggtaggtcg ttttgccttt
    42481 ggtttatctg gcttaattgg caaaggcgtt acttcctcaa ttggttcttc ctccttagga
    42541 atattatcca taaactgaat aggagcatta atcactgatt tcttaagctc gatttttaca
    42601 atcggctcaa ttcggtgagt tacaataaac agggtcttat tgcgagataa agtaagttta
    42661 tcttctgcag ataaagactc aatttcggtt aagctaacta ccttttctat tttaccggta
    42721 gctatatcta cgatttggac gaaggtctgt ttttcggcca taaagtaaac ctccattgca
    42781 tatttatcag gaaatttctt ggtgatatcc actacgaaat taagatattc ttgatgatat
    42841 aatttattcc aggaacgaaa actcttgcac tttataatac gaggacacac tgcacacaat
    42901 tgaggtgccg gttcgcgatt gcgttttatt tcacagaaaa agtgcctaat tattttcata
    42961 tcttttcctt atcttgtaat tcctttatac tgtcaagaaa atttatgttt tccaacaatc
    43021 cctccaataa tatctacaat cctgttcatc ttatacctat tatctttttg tcgaggggct
    43081 tacgccacca tcgctatcat tgaatttccc gttgggttaa taaggagaaa tatagtttta
    43141 taaaatcctc ttaatcctga aatcctaaaa tcttttttgc tagaattcaa attctaaaat
    43201 cagcccccta tttgagaact tgatagaaga ttacctcatc agaatgctca gtgtttataa
    43261 aatagaaggg gatctgcact tattttaaaa attcccttgc caaaaaccct ttatctaaaa
    43321 ttatgactta cttagtctaa atataaggaa acaaatatgg ctacaatggc tgatatcaga
    43381 aatgggatga tcattgaatt caaagatgat ctttatgaag ttgtggaatt tcttcatgta
    43441 aaaccaggta agggacctgc tttcttgcgt tccaagctga aaaacatacg cacgggaaga
    43501 gttctggaaa atacttttcg agaaagtgat acttttaatg aagttcgtat tgaacgcaca
    43561 aagaaagaat atctttatcg ggagggagat ttttttgtgt tgatggatag tgaaaactac
    43621 gaacagatgc atattgataa atctctttta ggcgaaaaag aaaagttact taccgataat
    43681 atggaattaa tcgtttctgc tactccggaa ggagaaatag taggaataga attgccaaca
    43741 acagttgttc aaactattgc tgaatgcgaa cctaatataa aaggtaacac tgcttctgga
    43801 agtggcaaag tagcatttac agaaacaggt ttaagactga tggttccctt ctttgtagaa
    43861 ataggggata aagtaaaaat agacaccaga accggtgaat atatagagag agctaattaa
    43921 aaagaggata aaatgggaaa agtaaaaagt tttgccgcaa aagttgcaca tgacctttcc
    43981 acttcaggga aagtgatttg cccggtatgc aatacagaag tgaagaagat taaacttatc
    44041 acgaataaga agacagatgg aacctgggca cctcagaagg aatatgttaa gatttgcaaa
    44101 tgcaacgaag cggatatcct tgcgggtaaa aatctgtaag gacaaccaat ctgcattagt
    44161 aaaggcgaat taattacgcc tgaggagaaa tgcgtctctt caggcgtttt ttcattgacg
    44221 gtttgtataa gctttataaa aaggttttca attatagcta tctgctttca gtagtttggt
    44281 agcggatttt ttgtcttcat aaatttattt tcctggagga ttgaaatgcc ttgccttatg
    44341 tggaagcgga tagttctact gctaacaatt gtaatagctg gttctttatc agctctggtt
    44401 cctcctgttc ctaaatataa gaatctacct gaaacttggg aagctacttc tttggaaggt
    44461 aatttttata caatggataa ttttaaaggc aggaagccaa atttacctaa taatattttg
    44521 gctataagag tccaattcaa tgatgtagcc tttcgttctg aagcagtata tcctgataat
    44581 atagcccacg atgatatctt ttttgatcgc tggatgctcc atttgaagga tttttttctg
    44641 gaagcgagtc atttacagta tgaacttgat tactttcttt atccgcaagt gcttacaatg
    44701 ccgcatcctt tatctactta cggtgccgac agcacagaag atatagatgt aaaccttcct
    44761 cagattttac ctgatattat ggatttaata gatgaagaag tagattttac tcaatatggc
    44821 ggaattatta tttttcattc tggaacgggt caggaatcgg atatagagtc cattcgtcct
    44881 aacgagatat ggagcacttt cttaacccgt aaaatgctgc agaaatattt tgagccggag
    44941 aatgacttat atcctggttt tattaccaat gatggagcta ttttaacaaa tgtaattatt
    45001 gtccccgaag atgaatatca ggattatttc ccaactgaag gtgtagatga tgcttcgtcc
    45061 tatcttttta gtatgtatgg agtattagct caccaatttg ggcatatttt aggtttgcct
    45121 actttatatg ataatgacag cagtaatgga atcagccagg gaattggtaa ttggggttta
    45181 atgggcacgg gtttatggaa tggtaatggc tatgttccag ctcagctttg tgcttggtct
    45241 cgttattatt taggttggga caatccagtt atcatatctc aaaatagttt aaataatgca
    45301 gttgatcatt ttctaaatca ttcgcctaca gctatacatc tttataaagt gccaatatcg
    45361 gcaacggaat attttttggt tgaaaacaga caacaaaatc ccgatggaag tttagaccct
    45421 ataactaata gccccagtta tagttttaag cttcttccgg agggtgagca ggattactat
    45481 cctgatgatc cttctacacc acaggatgaa tctctgctac catattttaa ttttatggaa
    45541 aacagctatt taggcagtga atgggatttt ttcctacctg gtttgggagg tccgattccc
    45601 aataatagta catatcttgt agatggctcc ggaattctta tctggcacat agacgagaat
    45661 gttatcaatg caaattttac tgcgaatttt gataaaaacc gggttaatgc catttctgct
    45721 cataaaggag tagatttgga agaagcggat ggaattcaaa atatggatac cggtttgaat
    45781 agtatttaca aatggggaag tccttacgat agctttcgag atgacaacaa caattatttt
    45841 ggtcaccaat atcataatgg agtgctttca ttgccaactt cagaaagcta ttatgggggt
    45901 attcctttag aaatatgcaa tatttcggcc agtggattac agatgacttt ttccgttata
    45961 tacagctggc gtttaaatac ttcctatgaa ggaactaatc ctattaatgc agctgggata
    46021 gattttgata atgatggaac tgatgaactt ttctatccta tgcctgatgg taggatttat
    46081 atttggaaaa atgaagagct gatggatggc tatcctttat atggaatgcc tattccctat
    46141 acttatactt gggatggaga aaatcttttt attcctatgc aacgggatcg gctttgccgt
    46201 ttatataaac acaataaaaa tgaaagcaaa tatgtttata actctcctaa ttacagttgg
    46261 gttgggcatc ctgtagattt aggaaattct atcgctatgc ctttaaaatc caataatgaa
    46321 gagaatacct tttctcttca gctttacagt aaagaggata atagcgtaaa tacaattgtg
    46381 gatttgtctg gtaactttaa agccaacctg gtatattttc ataacaaact ttccgttgtt
    46441 tatgaaaaca catcgggaga atattggcta agtgatattg acttagcgga taatacaata
    46501 actgattcta agcttcctat ttctgccgat tcaacaatca tagcgatctt taaagcgcca
    46561 gttaaagatg atgaaaactt aattgtacag tgccaaaaca gtgtttattg tttaaaggga
    46621 gcaaatatag ttgatggctt tccctttgta cataatctat ctgccaattc ggacagtagt
    46681 tttattgctc ctttaagcat agccgatgtt gatggaaatg gtgctttgga tattttgatt
    46741 ggaggggaaa gtgattatgc tgttattgat tatgctggca gattaatgaa tccagaaaat
    46801 ccaagtcctc agcctcattc agattttatt tctgctggaa tattggcaat ggatatagac
    46861 aacgataata aagccgagtt gataggcaat tttaaaaata accgccttaa gatatgggaa
    46921 aatgattttc gcgataaaag aggttatcct gtttcttttg cagagcgcag tagaacctta
    46981 ccttttgttg ctaaggcatc agataacaat tggtatgttt attgtgccac ggataatggt
    47041 tctctgttta gaaatcaatt ggaaggagaa cctaaattca gtgcagctta taattggatt
    47101 agtgaatatg ggaatctaag aagaacggct tcaattgaag ctactgattt acctaatcaa
    47161 tatcagacca atgaactgtt tgtgcctggt gaggtctata tttatcccaa tcccttaaag
    47221 tccatatatc ggcaaaaaat attattgaat gtaatgacca atcgcgatgt cgaactggaa
    47281 ctaagtatat ttgatatcag cggtactttg gtataccgtc agaaaggttt tgccaaagca
    47341 tatcttaaaa atcttgatat ttttgatatt ccttctgcca aactatgcag tggggtttat
    47401 atagcaatat taagatcctc agagggcagc aaacgcctca agtttgctat agaaaaataa
    47461 cgggagcgta atctaatgaa acagatattt actaccttat gcctaatcct gatttcagca
    47521 atgtgttttg ctggaattaa taaagatgcc ggaacttacg gctataaatt tttaaatgtt
    47581 ccttatggac cagtggcttt atctctggca ggaagaggag tgtatagttc taataattca
    47641 tcagctttca ttttacaacc tgcttcttcc tgtgttaatg atcaacgcct tttgggaata
    47701 actcataata tgtggttggc agatacccag gcaaatatga tatcctattc ttattcccga
    47761 aaaatagatc attttggcat tgctatccgc aatttggatt acggtgatat agaaaacaga
    47821 gatgatatgg gtattatgat tggtagctat catccaatag atattgatgc tacggcaaat
    47881 tatgccagac ggctaagtcc taacttttat acgggtgtaa atttaggagt tttgtatcaa
    47941 aaattgaata cagcttccag cctagcttta cattcagacc ttggtttttg ctatcttcct
    48001 cctgttaaaa acaccaaaat atctttagca gttcgggatt taggggtggc aaataaaact
    48061 gatgatgaaa gagtaaaact acctactagt tttgagatgg atatcaacaa gggttttctc
    48121 ttagccgatc agcaaatttt tttggggggc tcagctattc agaccttgga tgaagacctc
    48181 aaaggtagta tcaatctgga aggtaatctc tttcaaatat tttctataag gggaggctat
    48241 ctgttgggtt atgatgctca ggattttagc gctggttttg gagtgaaata taaaaacatt
    48301 tctgtagatt atggatatgg agcttttaac agtgaattga acgatgttca ttcttttgga
    48361 gtaacttacc gattttaaca gaggtataag gtgaataata atcttggttt tatgcagaac
    48421 cgcagagtcg gattagtatt tttgttaata tgtttggggc tctttctcca agcagaaatt
    48481 actcctaatc agattatatt taaaacaact gttccagtac agattaaagg aacaaaaaca
    48541 ggcttaaatg cctttgatag tttccttact cagcaaggag ttaagcaaat aaagccaatt
    48601 aagggtatgc accaacccaa ttactatctg gctaatgttt ctgaaatgcc aagcattcaa
    48661 actttgcaac agcttagctt ttatggcatt caatatgttc agccaaatca cataagtaaa
    48721 ttacatacta ttcccaatga tcctttatac aatcaacaat ttcattatat atgcagcatt
    48781 ccagatgcct ggaattatac aaccggcagc aaatatatca aaatcggtgt tgtggattct
    48841 ggagtattaa ttcaccaccc agatttatgg caaaatatag ctataaatcc aaacgaaata
    48901 cctgataatg gaatcgatga tgataataat ggctatatcg atgactggtg tggatgggat
    48961 tttactgatg cccctgagat ggctgaaaat gctattggag attatatcgg acaggataat
    49021 gatgttgaag atgaaaatta ccatgggacg catgtatcag gtattgctgg agcagttgga
    49081 aataatggaa tagggattag tggagtttgc tggaatgtag gtattttacc aatcagggca
    49141 ggtttcagaa ctttagataa tcaaggttat ttacaggatg atgatgtttc tgcagcgata
    49201 atttacgcta ccgataatgg ttgtaatgtt attaatatga gttggggcga tcctgattat
    49261 tcagcaataa ttgctgatgc ttgtgaatat gcatatagca agggtgttgt tttaattgcc
    49321 tctgcgggaa atgatggagt ttcaggttta agttatcctg cgaaactttc tacagttatt
    49381 tcggttggtt ctgtgaataa tgctaaacag cgttccacct tttccagtta cggagtggat
    49441 ttagatcttg ttgctgtagg cgaaaatgtt ctttctactt ataaacttga tccaggagaa
    49501 caatattttc ggttgaatgg cacttctatg agtgcagctt ttgtctcggg agcagcggcc
    49561 ttacttttat ctttgcatcc agatttgagt cctgcagaag taagagcttc gttattaact
    49621 tctacggacg atatatattc tgttggtttt gatattcaaa ctgggcatgg tttgctaaat
    49681 gttaaaaaaa tactggataa tcttgctccg ccctatctgg aaattactag tcctcaagat
    49741 caattaggga taagtggttc agtaccaatt atcggttctg tttatggacc agattttgcc
    49801 gattatacag tatgctataa attggatgat gatgccaatg atccttactg gaaggatgtt
    49861 actactcacc aaatggatcc aattactcac ttggaacaag ttcataattc tgaattagct
    49921 actttttata ttccggatta tttcccggaa gggaaatatc tgctccggtt gcaatatgaa
    49981 aaaaaaataa ataatatgaa tcgctataac tattacagga ctgtttatgt agatcgtagt
    50041 gcaccagtta taaggtcgga aacggtttct ggatttactc gttggaaccg ccaaaatcta
    50101 caatattatg tttcagcaaa tttcaatgag aaggtaaatg ccagtctgaa aataactgct
    50161 tccgatggcc agatatacaa tgtttattct actcttgcag atagcattca tatttttaag
    50221 ttaccttcct atattcctga agggatgatc agcgttcagt ttgttgctac taatataacg
    50281 ggtttaactt ctgtctctga tactttcaat gatttgttga acattcacta ttgtttaatt
    50341 ccacattatg gttatactaa agaagtaata ggtcctcccc aaaaaccttt actccgtaaa
    50401 agagattata atggcaatgg cgtttctgaa tatgtttcta tggaaattcc agcgagtgga
    50461 tatggtcccg taaaagcatt tgaattgtgt tctggagagc acatcgaaac taatgatttt
    50521 ggggatgctt tttggccttt ggatattggt tccacctata caacaggttc caagagtgaa
    50581 ttgttattgt taagagtaga aaatgctgaa ttgtggctta ctcctttagg aggaaattat
    50641 ccttcgaata gtaacgatag cttagtaatt gcgttggaca ctggaatttc cggaggagta
    50701 atggccgact atgataatga tgggaaaacc gagattttgt tggttaaaaa tctacctgcc
    50761 gaacgcgtta ttcaagcata caaaagaata tccacaggtg ccctggttca aagaaacacc
    50821 ctcagcaatc ctactacgac ttatagtcgg aataattttg tgcctaccat cattgtagag
    50881 aatctggatg gcgataacta tcccgatatc ttatgtgccg atacagatgg cgacataatg
    50941 atttatgaaa ttattaatcc tacagaacac catttacgat ggacaactcg gttgcccgta
    51001 ggcaatactt atattcttac atcaggggat tttgatggtg atggcaacaa ggatttcttt
    51061 gtggcaggat ataatacaga cataactaat ccaaatctta gtttctggta tggcgaaggt
    51121 tttcgtaata caggaaataa cacatatagc tctatgggca atattatgtt caatgaggtc
    51181 tcaactttaa attctattac taatgcagac ctggataaag acggttgtaa cgagattatt
    51241 ttagcgatta ctcccaattt atatattctt aaatatagca acggtaattt tacccctgta
    51301 tattatgatg aaagctatag aaattaccaa gttgtcactt tggaagatga aaatggtgct
    51361 gtgcggatta tggccaatat acatagcgat ggagataatg ctgtttctgc acagtggata
    51421 ccttcagcat catttacagg acccgcaact ccagctaatt taacagtgaa acctttaaat
    51481 gaaaattcta taaatatttc ttggatatct acaggtgcac cggaatatgt tttatatcgt
    51541 aaagatgaaa ccggaaacac agtttcgtat aatttaagtg cagcaaccaa ttataccgat
    51601 actggtttac aacaggggaa agcatatcaa tatgcaattg cagctataga tccatcttac
    51661 actcccagtg aaagtaataa aggcccttgg atagaaggta ttcctttacc caaaccggat
    51721 gttatagaaa taaaccttgc aggacaaaaa gagctgcgtt tgttatttaa tcaggcaatg
    51781 cccaatgata ttattaatcc aggccattat tttatcagta acgggctggg gaacccaatt
    51841 tctgctaata gtatttccgg aaattatgga gtccagctaa gatttcggaa taattttcca
    51901 cctattgatt ctctgtttgt aattgagcta agaaatttaa ctggaatcag tggtgttgat
    51961 cctgtacaaa acttttatac tttttcttat caggaagatg taattcctcc agaaatatcc
    52021 caagtgaatg ttctgccttc ccaacgcgga ataataataa cattcagcga agaaatattg
    52081 cccaatccag ctttgcatct tgccaattat atattacact gtccacaaaa tgatcccgat
    52141 aatacaattg tttccatagc tcattctgta gatacggtta caatcacttt tgccgaaaag
    52201 ctgaaattca ctaattatgc ttactacctg gaaattaata atataaccga tttagcgggt
    52261 aacttaattt ccccgcaggg caatttagcc cgcttcggaa ttcaaaacct caataatctc
    52321 gatcacttaa atgttttccc caatcctgta acttccaaac atcctcagga agctactttt
    52381 attaattttc ctttaaacaa aaaagggaaa attgctattt acaacagtgg gggacagcta
    52441 gtttttaaag atgatatcgg tccttttttt gaaggtagta ataatattac atatacttgg
    52501 aatctaaaga ataatgatca gcgacctgtt tccagtggtg tctattttta tgttgtagag
    52561 atgggtggaa aattaaaacg cggcaaactt gccataatta aataagctat gtccttgcta
    52621 aaaaacacac ctggatttac accttattta tatgtaaacc gtaaaatgag ccgcactcta
    52681 aataaatata acctgataaa aagattttct gctaccttga tatgttttgg aagataataa
    52741 tatctgtgtt taattctcct gtatacactc ttgtgataag cactatattt ttattgctta
    52801 atatcctgga tggacattca acttatctgg ttctaaaacc aaactattat catcgcgaaa
    52861 aaaaccctgt tgccagatgg gtatttaaaa agctgagtat tcccaatgga ataatcatct
    52921 ttaaaactgt gctaatggct attttgattt tggctatttc ctattatacg gcttgggatt
    52981 cgttaactat aaatatagcg atgactgttg cggatctcct ttttctgatt gtagttatac
    53041 acaattaccg tctttaccgt cggataaaac attagtcttc tctgcttact aagcaagcaa
    53101 gtaatttttg gtatcagatg ttcctaaggg tggcaattat aaatatttct ggattttacc
    53161 ttctattaat cctttaattt cattcatcct ttcttctgta attgcttcat aacgcgtaac
    53221 gattattggg gttgtattgg aagcgcgaat caaaccccat ccatccgaaa aagttatcct
    53281 ggcaccgtcg atatcattta cttcatagcc ttctttctta aattcttcac aaaccttggc
    53341 tactacttcg aatttttttg catcgggaca gcttgtatgt agttctggag tattaaaaag
    53401 tttaggttga tcagccaaaa aagaactaac cggatttaga gtttgcgcta caatttcaca
    53461 aaaacggcag cttacataga tggcatcatc aaatcccaaa tatctatctg ccaaaaagat
    53521 atgcccactc atttcacctg caaattccac tttcttttcc tgcatataca tctttatatt
    53581 ggcatggccg gttttataca taatcggttc accgccatac tttataatgt cgttaaatag
    53641 attcatactg catttcacat cggcaataat cttcttaccg gggttatgtt tcagataatc
    53701 cctggctagt atattcaata tctgatctcc taacaataat ttaccctgtt catccacaac
    53761 tccaatgcgg tctgcatctc catctaaacc aatgccaagt tcatatttat tttctataac
    53821 ggctttagat agcgcttcca tatttttggc aatagtagga tcaggatgat gattaggaaa
    53881 atccccatcg ggctcacaaa acatatctat cacttcacaa cccaaacggc gtaaaatagc
    53941 tgttaaataa ggtccgccaa ctccatttcc tccatctaca atcactttaa ccggacgctg
    54001 taatttgatt ccattcacta cataatcaat gtaaatatca cccatatcat tgtttttaac
    54061 ataattaccc tggccagaaa caaaatctcc cttttgaatt atttgtaaca ctttttgcaa
    54121 ttcatcagca taaacactgc ttaaaccaag atttaattta catccattat aacgaggagg
    54181 attatgacta gctgtaacca ttgctccgcc atccgttttt agcttccaga tggaaaaata
    54241 taaaacagga gtaggaacaa ttccaagatc aataatatca caacctgttt ctaaagcacc
    54301 tttactaaat gcctgcataa aagagggggt tgaatgtctg gcatcaccac ccaaaacaat
    54361 agtttttagg tttttattcc gtatataggt tccaaaaccc ttaccgatta aataaaatga
    54421 attctcatcc agttcttcat ttacaattcc acgaatgtca tactgacgga atacttctgc
    54481 tttgatcatt gataaactcc tttaatcagt caataattac tttctccaaa atgggtataa
    54541 tagctttcat cgccaaggcg cgatgggaaa ttttattttt ctgttctgaa tccatttctg
    54601 cgtaggtttt atctcccaca gcaaatatac tgtcgtaacc aaaaccgtta tcgccaagtt
    54661 cttcagtggt tatttcacct tccagttttc ctcccagaac agcaacaata ccatcaggaa
    54721 gagctaaagc tacaactgtt tcaaaatatg ctttacgatt ttctacgccc tgtaacaggt
    54781 ttagtacttt tttacgatta tcagaatagc tgcaagaatt accagcaaag cgagcagata
    54841 aaatacctgg agcaccattt aattcttcaa taaataatcc ggtatcatct gctaaagtaa
    54901 gtaaaccgct atattttgcc gcttccaaag ctttttgcat cgcattttcg taaatcgttt
    54961 taccattttc ctcgctggga agaaattgag gtaattcatc caaaaaatgc aattgtacct
    55021 tcaggttatt tagtagttcc tgcagctctt ttagtttatc aggattgtga gtagcaacta
    55081 ggatatcact aatcatatct tttttaacac attcttgatc ttattcaata aatgcttatc
    55141 caaaaattgc tctgctaaag ctgtaatctt ttttaagtgc tccagaactt caccttcgcc
    55201 actccaatta tctaacacaa tttgaggatt aagttcattt aaaatcatat tcaaaccaag
    55261 aacaattaaa accagatcat ccacaaaacc caaaaccggt ataaaatctg gtataaggtc
    55321 tatcggcatc atcacataag caataacgcc tcccatcagt aatttttgtt ttacgctaac
    55381 ccgtttatca acagctatgc ggcaaaccaa aatgaaaaaa tctggtaata aaaatacata
    55441 ttcacccagt tttccaccta agggacctaa ttgggtatta gtccaactcc ttactttctt
    55501 acgcaggtct tcgtaaaact gtaatttcgt ggaatctaca tcatcatcga tgattttatc
    55561 tttaatcttt tcctgctctt caacaggaat ttccttaccg atgattacat catcttcttt
    55621 cataataaac ctccttttac ttatataata atgataagtt tcaggtcaag caaattattt
    55681 tggatccaac agtttgcgct tattataaaa aagggggata tcattttcca ggttggttga
    55741 tatccccgtc tataatttct aagttacctt atctactaat agtaaccata tatcctaatt
    55801 ataattgaac cacaaagatg tagttcattc aatttattca ctaatagcag ctttggctgc
    55861 agcaacacga gcaataggaa ctctataggg tgaacagctc acatagttca ttcccaccat
    55921 atggcaaaat tctacactac taggttcgcc accatgttct ccacaaattc caattttcaa
    55981 ttcgggacgg gtttttctgc cgcgttcaac cccgattttt actaattggc caacaccttc
    56041 tctatcgaga acttggaaag gatcttgttt cagcagattt ttctctatat atatcggtaa
    56101 aaacacacct gcatcatcgc gagaaaaacc aaaggtcatt tgagttaaat cattagttcc
    56161 aaagctgaaa aattctgcat gttcagcaat tttatcagca agtaaagtag cgcggggaat
    56221 ctcgatcatt gttcccacca gataatccac tctatcattc atatcagtaa aaactttttc
    56281 ggctgtttca cggatgatag tttcttgcat ttcaaattct gcagggatac ttgttaaggg
    56341 gaccataatt tctggtaaaa cattaattcc gcgggcttta acagctaaag cagcttccat
    56401 tattgccctg gattgcattt cggtaatttc aggataggta tttcccaatc gacaacctct
    56461 atgcccaagc ataggattaa cttcatgtaa agaagcaact ctggctttaa ctatttccag
    56521 cggaattcct aattctttag caatttcctg ttgagcttct tcttcttgag gaacaaattc
    56581 atgtaggggg ggatccaaaa gacggatagt tacaggaaaa ccattcatag cagtgaagat
    56641 accttcaaag tcactcctct gcataggtaa taattttttc agtgcctctc ggcgtccttg
    56701 ttcatcttca gctaaaatca tctcccgcat cgagttaatt cgttcgcctt caaaaaacat
    56761 atgttcagtt ctgcacagtc ctattccttg agcaccaaaa tgtctggcta cagatgcatc
    56821 ttttggagta tcagcatttg tgcgaacttt cattctggta tatttatctg ccagttgcat
    56881 aatagctcca aaatcaccac tcagttcagg atcttgtgta tctattttac cttctaaaac
    56941 ctgaccagtt gagccgttta atgaaatcca gtcaccttct ttatatgttt ttccatttac
    57001 agtcatagtt ctgtttttat aatcaatatg taattttcca gcaccagcaa cacaacattt
    57061 acccattccg cgggcaacaa cagctgcgtg agaagtcata ccacctctgg cagtaagaat
    57121 accttgagcg acagacattc cccttaaatc ttcaggtgaa gtttcttctc gaactaagat
    57181 aactttttta ttattttcag cccaggcttc agcatcatct gcaaaaaaga caatctgtcc
    57241 agtagctgca ccaggtgaag caggtaaacc taaagtaata acttttgcct tagccagacc
    57301 ttctttagta aaaactgggt gtaaaagttc atcaagtttt gcaggttcaa tacggtttag
    57361 agcagttttt tcatcaataa ttccttcgcg taacatatcc atagcgattt tcaccattgc
    57421 tgcacctgtt ctttttcccg ttctggtttg taagagccag agcttacctt cttggatagt
    57481 aaattcgaga tcttgcattt cgcgataatg attttccagc tttttctgaa tttcgtataa
    57541 ctctttatag gtttcgggca ttgtttcttc taaagatgga tatttttcct ttctttcttc
    57601 ttctgagata ccggcaagtt ttgcccagcg ttgagaacca attttggcaa tctgttgagg
    57661 agttcttatt ccagcaacta catcttcgcc ttgagcatta attaaatatt caccattaaa
    57721 taaattttca ccggtagcag catcgcgtgt aaaggctacc cctgttgcac ttgtataacc
    57781 catattgcca aaaaccattg cttgaactgt tacagcagtt ccccattcat caggaatatt
    57841 attcagttta cgataaaaaa tagcacggtc attattccaa ctatcaaaaa cggcaaaaat
    57901 tgcaccccaa agctgttccc aagggtcatc aggaaacttt ttatgtattt tttcctgcac
    57961 ttcttgttta aaaagtgtta ctaattgttt aagatcttcg gtagttagtt ccagatcgct
    58021 gcttattcct ttttcttttt tcacctgatt aataataact tcgaagggat catgttcttc
    58081 tttattggca ggcttcatac ctaaaaccac atcagaatac atttgcacaa aacggcgata
    58141 gctatcccag gcaaatctct cattattggt ttttttaatt aagccctgaa ttgtagtatc
    58201 attcaatccc agattcaata tcgtatccat cattccaggc attgaagcac gtgctccaga
    58261 acgcactgaa accaaaagtg gattttcaac agagccaaat tgcatatgca taatatcttc
    58321 aatatgcttt atagctaaat ggacatcgtt atctaagatc tctctaagtt tggccgcacc
    58381 caatttgttg tattctatac aggcttctgt agtaatagta aatcccggag gaaccggaac
    58441 cccaattaaa ttcatttcag caaggtttgc accctttccg cctaaaagat ttttcatatc
    58501 agcacggcct tcggcttttc catttccgaa gagataaaca cgcttaggtg tattcattta
    58561 tcctccctat tattatcgtc ttatgatata ttatatgttt tgtcagattc ttcgatatgg
    58621 caaatttgtc aactgctttc ttggattatg gatatatcca agtaaaaaac cagagattcc
    58681 tgtaaagttc attatattca gcataattat aggtctaaaa aggtatttac tgctatttct
    58741 tttaagctca tagattttgg tatttttctc ctttttccta ttattcataa ttttttgctc
    58801 tttgcagaaa ggaaaagaag gcaatagggg gctatataaa aaaaggaaat atagaatata
    58861 gggcatactc catatttcct aaaaaaaact gttcgctgat gagtaaaatt gcaatgcagc
    58921 taaacagctt caggatagtt atccagctta tttagctaat ttttgcaatt cctgataaag
    58981 ctgatttaat tcttcttctg aaagcaccat atcaaattcg ctttcttcat tttcttcagt
    59041 taaattaaaa gcagtttcca aagcagttaa tgcttgttta tattctttta attcatagag
    59101 agttttggca taccagatat aggcatcata atgctcttca ttggtttcaa tagcttttcc
    59161 cagagcaatt ttggctgatt caaaattccc acttttatac agtgcttcac ctttatgacg
    59221 ccaggggcag ctccacaaag gggttgcttc attcagaagg tcataaattt cgatcgcttc
    59281 aggatattta tccatttcca gaaatagatt agccaaagca aagcgttcac ctggtttcaa
    59341 attatccagg ttttctttgg ccaaatccag taaacgcaag gattctaaat tgctaatata
    59401 ggcaagattc aattttgcct gcattccggt ttcaccatct gtgtccatct ttaccagata
    59461 atctactgct tgtttataag tatccaaatt ttgattttgc agatatttgg ttactggaga
    59521 ttcctgtgca aaacatagat ttaacaacat caagccgaaa gcaatataga taattttttt
    59581 cattgtggtt ttactccctt attcatattg gcaaagatag gcacttggtt cttccacttt
    59641 taaatggaat ttttgatgag ctgctaccgt gaaagtttct cctttgcaga acttctgcca
    59701 attttccttt tcaggtaagc gtacaataag ttctccggaa ataactgtca tcctttcaat
    59761 agtattagta ccgaattcat attcaccaat atccatcact cctacagtga agttaccctg
    59821 cttattttga aacgcaatgg actttacttt gccatcaaaa tactcattaa tagtgaacat
    59881 atatgtctcc ttgttgaata tagttttagg cacataatct aaaaaatcca aaaatcatat
    59941 ataaaaaaag tggctccgga agaggggttc gaacccccga cctagtggtt aacagcc
//
LOCUS       DAOSTH010000007        52990 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_703_length_52990_cov_11.733749, whole genome shotgun sequence.
ACCESSION   DAOSTH010000007 DAOSTH010000000
VERSION     DAOSTH010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 52990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52990
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_703_length_52990_cov_11.733749"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..5529)
                     /locus_tag="PLB65_02345"
     CDS             complement(295..5529)
                     /locus_tag="PLB65_02345"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOQ77396.1"
                     /translation="MKRQFIAMLMLLLIISLFAYAPLNSKIDNKPSYASDLIKIKLGA
                     EVVYRANLPTDLYAEKTNTGINELDQLMAQTGATKIIKAHRKVNDANWEKQTGFDRWF
                     ILKLNGKTTVEEALKQFIANRYVEEAIPEYIAYPTVVPNDTYYANNWGHNNTAQLPAY
                     TSSGHTGSGVGTIGFDSDAQLAWNQSQGYGSASIIIAIIDSGVDTAHPDLRLVTGYDF
                     GDNDSNPMDNSSDPGHGTACSGIAAGKANNSLGIAGIAGGCSIMPLKVANSEGVNYFT
                     AIENALTYAADHNAHIASISLGATDIIEGDSPSTDAALNYAYNSGVVLFAATGNENNS
                     TISYPANETSVISVGAASPSGQRKSTTSSDGEYWWGSNYGVNTQNNKNAVDIMAPTIL
                     PATDLTGTGNGYNTNGDYYLWFNGTSCATPYAAGVAALLLSKDPSLTPAQVFSKLTST
                     ATDMTIDGGVGWDRYTGYGMVNANAALNTIVSGMPTCQITSPADGASFALNSIITINV
                     NATDSNGSIASVKTYINNILYNTDYSDPYSYNWNTTGLTAGSYTIKAVAKDNSNNETI
                     SEISISLIQPVSEAIIGTGTSVTGNSAPSPINVWYKSLHGQSVYTKAELNAAGIFGPI
                     NITQLGFNIVSLPTVTMPNFVVRLKHTTATNVSSFVTADNLVTVYSNPSYLPTQTGWN
                     MYNFTTPFLWNGTDNLLVDTAFGIASSYNSSGTVQYTSIDNGYHYLVNDNSNQTNIFS
                     GGSSSSTRANIKLKVAPNYTGPEITVTPNSLNFGSIPIGESQTREFTIQNYGDQTLNG
                     TIFTPAGYTVSESTRDNLQSSESNASSRNILDIAIVAGATKTYALIFSSPSAGSFDGV
                     VTISSNATNNPLVNIQVSGSVYIPPEIAVSINELAVTVYAQTDSTETFTISNTGGLPL
                     SYYIYPETRSRFTQNKLPEDKSIQGSTLTLNYAYYEPGTTINWTFTLYNNSSDLEWLK
                     DVYLTFPADIIVNSASNFTGGTDDMLPNISSGDGITIHWHGESSNGYGVIQGGQTATA
                     NINITIPLTFNGNLILPYTIQGDVYGGEPHILNGQLILNKIIHTIPWLSIEPSQGDIP
                     VSGTTTINVTFSAQELVAGLYNAQLLVNSNDPTNPLTILNVFLTVLPVNHPPVINLPA
                     SFSFDVNETLTVDFSTYVSDADNDPLIITCSEANNVIATIDGSQVNLSATHNWFGTET
                     ITFNVFDGICQSSDSVLVNVLINISDGFIVDKDNLPTNWTSENSPYHIVEPIIIASTK
                     HITIEPGVIVQIWNDEPINVYGSISAQNVTFMPGFSDLLWSGLAISRTTGIGQESNFT
                     NCEILNALNPITVLNCNPIIHSVQIAPIDATVLINGTGITLSGNTAANISNVEISHYG
                     TGILINGNANPILRDAKIENAEIGIRINSGGNLDMQNSMILNCKTGLQNFSSVFNYIE
                     RNLFVVDDLQISPPDLQNYLALSINAIPAFTFQNNTIYGYPNIATVNSAQLNFSNNIA
                     WSNTPISVPFVCQEGTVNAVYNDVNYGTMVYPGIGNINANPHFANAIEHDFHLLYTSP
                     CIDAGSPVTTPDPDGSIADIGCYHYSHSASIITPEGPFFVGHPLQFINNSIGHNTPES
                     HPIWLVNGLPVSSEWDLHTVFNTWGNYAIRLIMISGSLIDSSDVFQLYVIDETPLAPQ
                     NVVLQSVENSYLLSWDAVTLSVSQTPITITNYKIYASEDPLGTFVLFQTMPASNRQLI
                     LNLSELGSKRFFKVTAEK"
     gene            5761..6699
                     /locus_tag="PLB65_02350"
     CDS             5761..6699
                     /locus_tag="PLB65_02350"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOQ77397.1"
                     /translation="MTSKPQFTGRSLFDLDDYSHEEIMYILEAAKGMKEINLREYKKI
                     PTLRGKTICTLFVENSTRTRMSFELAANRLSADVVSFQSSISSLQKGESLQDTVYTLN
                     AMGIDLYCIRHSSPGSPQLVNKYSGKPVINGGDGRHSHPTQALLDIFSIWEKLGDLKG
                     LKITIVGDILNSRVVRSNLIGMGKLGAKVTVCGPKTLMPGNMESVYDCRVEYDLPRAL
                     KDADVVMGLRMQLERMTEGLFPSLEEYSKHYVLSKETLKYAKRNALIMHPGPMNRGVE
                     ILPEIADSNQSVIVEQVANGVAVRMALMFLILGGKA"
     gene            6711..7997
                     /locus_tag="PLB65_02355"
     CDS             6711..7997
                     /locus_tag="PLB65_02355"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOQ77398.1"
                     /translation="MKILIKNAKIFQKDKFIKGDVLIERKKITKLGGTITVNADKIID
                     AQGACVFPGFIDLHSHLRDPGHTYKEDIVTGTKAAAHGGFTTVCAMPNTEPVTDNIAS
                     VEYIQLRAKDYGSARVYVIGAITKQSAGEEIAEMATMKSGGIVAVSDDGKCVQDARLM
                     LSCMKYASNFGLPIIIHPEDYSLAGKGQIHSGKVATKLGLSGIPGLAEEVIIARDIML
                     AESAGAQLHIAHISTARSLELVKNAKDKGLPVTCEVTPHHLVLNEEATITFNTNTKMK
                     PPLRSEKDRLACVQALKDGLIDCIATDHAPHADFEKEKEYDHAPFGIIGLETAFPVLY
                     KNLVQTGLISLNRLVEALTIAPAKILDLPGGVLEVGKAADLTIIDLEKETLFSVENIL
                     SKSKNTPWLNQFLPGRVLCTICNGNVTYMDSEKWTN"
     gene            7985..8776
                     /locus_tag="PLB65_02360"
     CDS             7985..8776
                     /locus_tag="PLB65_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HOQ77399.1"
                     /translation="MDKLKPQFLERAIYSREELCGDYFILWIWDEELGKKCQPGQFFE
                     IRAHYSIENAEETRSLPKLFKPISIYDNAEGRIGFFIKKVGYGTNALSKLKAGDLLEL
                     IGPLGNGFPIVSGKKILLVSGGIGYPPLWYLQKRLIKHNLIYWVHGGSNQCDVFPCDE
                     VWTEDGSLGKMGMVTEGVRSLIMEKKINLIYSCGPIPMLKECARIALELEIEHYCSLE
                     AYMACGIGVCYGCAVPIGNTEDWNYLRVCKEGPVFNAKEVLWNRM"
     gene            8761..9678
                     /locus_tag="PLB65_02365"
     CDS             8761..9678
                     /locus_tag="PLB65_02365"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HOQ77400.1"
                     /translation="MEQNVSQIYTSLGRLTLNSPVTIASGTFALENLDFMAQDYLGAY
                     VCKTITRYPKKGNPPPRLFETEAGLLNSIGLQNPGLDKFINDDLPILRETLTTPLIVS
                     FSGSSLNEFKLMLESLEQNEGIDGYELNVSCPNVENEGIAFGVDPEIVYKLCALLAPL
                     TNRELIIKLSPNVTDIAVIAKAAEDGGATSISLINTIWGMAIDIQSGKSMLKKGIGGY
                     SGIGIKPLALALTYRAAKAVDIPIIAMGGIYSWQDALEFFWAGASAVALGTANFIDPL
                     AVPKVYEGIDNFLRERNCTLNEIIGKCEV"
     gene            9886..11943
                     /gene="fusA"
                     /locus_tag="PLB65_02370"
     CDS             9886..11943
                     /gene="fusA"
                     /locus_tag="PLB65_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOQ77401.1"
                     /translation="MKEYEMKKIRNLLFIGASGAGKTTLAEQIFYLTKTSNRLGKIDE
                     GNTIMDFDPEEITKQMSLGLSIGYVNYLDHKINILDAPGCPDFVGDTIVALPAVENVV
                     IVANAAGGYEVGLELAIEQIEKSKMGKVIVVNRIDNEHSDYDKTLEAIAENTKIKPVK
                     VHIPIGKEGSFEGVIDIIKRKAIKGKTESDIPANMSEIVEEARMHLMEAVAETDEDLL
                     NDYLENMELPEDKLIAGLKKGIISGEISPAFVCSAGTGEGVLALLSGIIDYLPSPEDS
                     NEMEVLKENEKVSFICKPDGELLGYLFKLYADPSMGDFAYARIFSGTLKTGVEFYIPE
                     KDAKDKAGTMYYMLGKNRNDCNEIKAGDIGALLKLKNAKVMNSIVATGSKLSVVPVEL
                     PTPTTWQTLKAVNQADEDKIGTVLQRVIAEDPTINYELNVETHENVLSGMGEQQLQLV
                     LKKLKNRYKVDAILKDPRIPYKETIMASSESNYKHKKQSGGRGQYADVYFRIKPTERG
                     EGFKFINSIVGGVIPSNYIPAIEKGLIETMEKGIIAGYPVVDISVEVYYGSYHEVDSS
                     EIAFKIASSMALKEGFKKSKPILLEPINELVIIVPSEYMGDVMGDISTRRGKILGMEQ
                     NGKKQYLTAQMPLAEMYSYYPALKSFTQGRGRFTQKFSHYEKVPDDIAQKVIAAWQEQ
                     SNE"
     gene            12161..12916
                     /locus_tag="PLB65_02375"
     CDS             12161..12916
                     /locus_tag="PLB65_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOQ77402.1"
                     /translation="MSGHNKWSSIKHKKGAADAKRGQIFTRLVKEIILAARAGGGDPD
                     MNPRLRTAINTAKENNMPKENIERAIKRGTGEIEGAAYEEIIYEGYGHSGVGIVVEVM
                     TDNKKRSVSELRHIFSKYGGNLAETGSVAWNFEQKGFMNVPAAGLDEDDFMMKALEAG
                     AEDVELNENNFDIYTSPTDFHTVISNLEKEGFPIHNAELTRVPKNTIAVDDDIAEKLM
                     RLIDSLEELDDVQKVYANFEFSDAFMEKLSQAQ"
     gene            12921..13397
                     /gene="ruvC"
                     /locus_tag="PLB65_02380"
     CDS             12921..13397
                     /gene="ruvC"
                     /locus_tag="PLB65_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOQ77403.1"
                     /translation="MIIIGLDPGSRYCGYGLLQIEDRKILAAGCDVLDVTKGKTLSLR
                     LKSLYENIIAVLDEYQPEIAAVESIFFHRQVRSVFTLGHARGVILLALAMKDIPIVEY
                     SPREIKKSVCGNGSASKQQVRYMINKIYPLKESPKRDDAFDALAVATCYYNRMKWL"
     gene            13397..13996
                     /gene="ruvA"
                     /locus_tag="PLB65_02385"
     CDS             13397..13996
                     /gene="ruvA"
                     /locus_tag="PLB65_02385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOQ77404.1"
                     /translation="MIQYISGTLTFKNPVMAIIETMGIGWEIKIPISTYEVLPSVGKN
                     CKLLTFLHISQDDMRIFGFSTEAERELFMMLNKVSGIGPKIALSILSTLSIPTFIKAI
                     NSGEEGLLTRVPGIGKKSAQRLIVELQDDVHTLMSHIDQKDLQIGDKNSEVESALLSL
                     GFNLQQIRKELALLDESMRQLNTETLIKEIIKRIYQRNK"
     gene            13993..15309
                     /gene="rsmB"
                     /locus_tag="PLB65_02390"
     CDS             13993..15309
                     /gene="rsmB"
                     /locus_tag="PLB65_02390"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOQ77405.1"
                     /translation="MNFRTEAYSTILKVLKDKEYSDTLLHQRAKKIKNAGEDPALFYN
                     LVKGVIKLCLKLDYILQQYVDKKKFASTDVKIKTLLYMGLYQLMYLNSIPDHAAVNET
                     VELAKTLFNEQVADFVNAVLRSYLRNPEITYPKEPELRIAYEHSFPPELVKQWLELWG
                     EENTEYLAMFFNENPELHIRVNTTATTPDKLKAYFAKRDIILKPSAASSNMFYTAQTA
                     EVLEDVAFSEGYFSVQDTSAALIVELLDPQKEESVLDLFAAPGGKCSYIAERMNNTGE
                     VIAVDKIPSKMKLLKQAADRLQLTNIKTVITDAFKYGPVAPAFQRVLVDAPCSGWGVF
                     GRKSDLRWQVHQNIPELIKLQEKALDYAANFVAPNGFLVYSTCTMNPKENEDQIASFL
                     KRNKKFELVPASQFIPDKYTSSGFLKTIPFKHNMDGAFAAKLQRIK"
     gene            15272..15904
                     /locus_tag="PLB65_02395"
     CDS             15272..15904
                     /locus_tag="PLB65_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOQ77406.1"
                     /translation="MVHLPQNYNGLNKNRYLQGKGKTKNKQIGYLVGIGICVIIVTAF
                     VFSQIFFPIILGRTPKVEVPSVVGMNLAHAKKILQDEKLHAVVKDSLFSDVAAPEVVL
                     EQHPVPGDKLHQDGTVYLVISKGSSTVTVPSVVGKPFQEAFIILRNLDLHSTVVDSTY
                     SDIYPVNTVIRSVPSAGAKALKKSTVKLILSKGSEPTADTLDYEMIPYYF"
     gene            15936..16409
                     /locus_tag="PLB65_02400"
     CDS             15936..16409
                     /locus_tag="PLB65_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77407.1"
                     /translation="MNEELKEIKIRARMMPGAVTYDGFLGNDKRSLSEIIAADNKELE
                     RFNRYVEEIADRMKYFTDATFDVFDNKVVIDDIYEVESTSSKGNLSCPYGHGGLFRKY
                     ETTLTNRKNNITVKWTAMTIHLIEAHHFFGGKGSHYRYEPETLLKALFTEAERKK"
     gene            complement(16648..17856)
                     /locus_tag="PLB65_02405"
     CDS             complement(16648..17856)
                     /locus_tag="PLB65_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HOQ77408.1"
                     /translation="MKILLITYYFPPCGGAAVQRWLRFINALDKKGVEIFVVTTLDGD
                     YPYRDESLLSKIPATVKVLRSKPISFGKLWNILGQKELPYGSLQNKTTDKPLQKFLYW
                     LRLNVIVPDLRIGWNPAALKSAVQVLRTNKIDYVITTGPPHSTHLIGLKLKKRYNIQW
                     RTDFRDPMSEIYYLKLNPPMRLTMALHKYLERKIIRTAELNYIVSKGIANSLPEGNKE
                     VLYNGFDPDDFKDLHYERTNRFRIKYVGQLTAGQDPTPLINALTEIKLPNIDFSLIGT
                     RNFPETDFPVNRIPFLPHQLALQELINAELLILIINTYEGNEGMLTTKLFEYIASRSP
                     ILCLADSDGEAYELICKTESGFVSKNTEEIIEIINKLYERFSKGDNLRTKGDISFLDV
                     NNQIQALLKV"
     gene            complement(17856..18944)
                     /locus_tag="PLB65_02410"
     CDS             complement(17856..18944)
                     /locus_tag="PLB65_02410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOQ77409.1"
                     /translation="MKRIAILGPAPPYRGGISLFAFSLAEAYRDLGYQVQFINFKEQY
                     PQLLFPGKGQYDYTITDNEFVNKRILVPWQPKTWGQTIYEIKQYKPDAIIVSWFLPYF
                     APAYGYIIKRIPNTPIIILAHNILAHEKWILEKQLLKYVFKPATKIVTLSKATLEELH
                     NELPLYISKKGVLGFHPCYEHYISEVVPFPQRNTLLFFGLIKPYKGLDVLLRAMPKVI
                     NAVPDAKLIIAGEVYGKCDVYTELINSLGIGSKVECYFRFISDPEIAHFFSQSCLCIL
                     PYKSASQSGVIATSYSFGVPVLASNVGGLGEYVAEGETGYLVPPDNPSALAEKIILHL
                     TQKPDMRKAIDNFTKRYSWKALAELTLQ"
     gene            complement(18941..19936)
                     /locus_tag="PLB65_02415"
     CDS             complement(18941..19936)
                     /locus_tag="PLB65_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOQ77410.1"
                     /translation="MEKRLNMKKNILIILTGGTISMKASDPAGVIPSNEFADFLRQFP
                     QLNSVANVEVMDYLNIPSPYMTPEMMFELAKLVDLKIIDYDGIVITHGTDTLEETAYL
                     CDLVLTTRKPVIFTAAMRSGNEIGLDGPRNIIGSVRVACHPDSIDKGVLVVMNDEIHT
                     ARDVVKSDTGKIDSFISPGYGPVGIVDPDKIVYHRTSLYKDNVWTDQLDTKVELIKAA
                     AGMDGKFIRCCMENDIKAIVIEAFGRGNLPKSLVPDIQAAIAKSILVVITSRTYTGRV
                     LAEYGYEGGGKHLETLGCILASDMKGVKVRLKLMALFGKYHDVDIVKRFFKQSVL"
     gene            complement(19937..20605)
                     /gene="queC"
                     /locus_tag="PLB65_02420"
     CDS             complement(19937..20605)
                     /gene="queC"
                     /locus_tag="PLB65_02420"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HOQ77411.1"
                     /translation="MTRSIVLVSGGMDSLVTAACAVSEVDEVFFLHFNYGQLTEQKEL
                     ACFAALADYYKPFQAKVVDYQWLSEIGGSALTDRHIPVPEGKISSKEIPITYVPFRNA
                     IFLCAAVAWAEVIKATKIYIGAVEEDSSGYPDCREVFFKSFEKVIATGTKSELPINII
                     TPVLHKSKSEIVQMGIKLKAPFHLSWSCYSDDEIACGVCESCLLRKRAFREAGIIDPI
                     PYRN"
     gene            complement(21004..21963)
                     /gene="dusB"
                     /locus_tag="PLB65_02425"
     CDS             complement(21004..21963)
                     /gene="dusB"
                     /locus_tag="PLB65_02425"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOQ77412.1"
                     /translation="MTTYLFKEPLLFLAPLAGYTDSPFRQICKQWGAEVLISEMVSAD
                     GIIRDSDKTLQYAYFEEFERPFGIQIFGNDPLIMAKAASTLLPLKPDFIDLNMGCPVK
                     KVIRRGAGSALMQTPSLAVKIVKEVKNALQDCLPLSVKFRSGWDMQNLNFLDFGILMQ
                     DSGADFLCLHPRTSRQMFSGISNWEHIKILKQNVSIPVIGNGDIKTPEDALTMLRETN
                     CDGLMIGRGALGKPWLFSQCRELLKGGTYKPVTRQDILNTIILHIDKALQYKQEYIAV
                     RELRAQLCFYTKGILGGPELRNKINHTDSIAELKEIILKGFPL"
     gene            complement(21963..23651)
                     /locus_tag="PLB65_02430"
     CDS             complement(21963..23651)
                     /locus_tag="PLB65_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOQ77413.1"
                     /translation="MKYDFSTNYEINKMLNSVVDNITNLAEEQLLHIKRLTRIGESLS
                     SESDLDKIFDMILEEGISFTKADAATIYKVNDEATKLEFEIVYNATLNLLKGGTKGPI
                     NWKAIDLYNEDGTPVNDKIVTNVFHNKRSLCFDDVYEAKGYDVSGTIKTDQENNYRCK
                     SMLTIPLKNHEDRVLGVIQFINAMDENSNIISFTEEHKAMLSSLASQAAIALSNKKLI
                     EDLEELLNQFIRSIAGAIERKSKYSSNHITRVALLTEMIANKINDIGEKYFPERKFSE
                     NELKELSMAGWMHDVGKIVTPEAIMDKSTKLETICDRIELVKLRIEKLELLLSYLQVK
                     LSESDFADFIHHYIDLELEPKDFHSYLEDLTNFLINVNEGKEFVDKTDLERVDKFSNI
                     DFEYEGKHYFIFTADEKKNMMISGRGTFTPEEMKIMQDHVSITWEMLSQLSFPKKYEN
                     VAFYASTHHEMLNGKGYPKGYSADNLPLQSRILAVADVFEALTAADRPYKKAKTLTES
                     LTIMGFMVKDGQLDKDLVSFFLDSGLYKEYAERFMKREYIDEVDINAIKAKYSG"
     gene            complement(23657..24289)
                     /gene="trmB"
                     /locus_tag="PLB65_02435"
     CDS             complement(23657..24289)
                     /gene="trmB"
                     /locus_tag="PLB65_02435"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOQ77414.1"
                     /translation="MINHKDFFVIEPKINTEGHDLRLDPQEIFHNSNPLLLEIGSGKG
                     EFISLFSVLHPEFNFIGLESAEKRINNTLKKLSPEQNPNVRLIRLHIDANISQLFPAE
                     SVSGVFIQYPDPWPKRKHHRRRLIQQDFLSALSIILKPSSRVQITTDNRDYAAWILDE
                     FLTHPSFISVYDEPVLKHSPFEEHISTFYEIEQKRQGFEPQYMLFQRIQE"
     gene            complement(24282..24899)
                     /locus_tag="PLB65_02440"
     CDS             complement(24282..24899)
                     /locus_tag="PLB65_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77415.1"
                     /translation="MESTKIMPRHLLLLFFPIATAILGGLLFSGSFRDFTNNWGGRGI
                     SQAELYLAVSFFIGAIAFSCYCLPKYALWGLLIVIPLLDGILMIVRLGSQFSLILLFI
                     LNLICGILLWLILRYTFLAKSLIKIRTLIFSFVSALVLGTYLKILMLLLKQPKAANTF
                     MDYFLNALVLFIFIGVGISLANLVIVRKEIDEAKKAIREDAEDDD"
     gene            25053..25970
                     /gene="ftsY"
                     /locus_tag="PLB65_02445"
     CDS             25053..25970
                     /gene="ftsY"
                     /locus_tag="PLB65_02445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HOQ77416.1"
                     /translation="MKSLKEKLSKTNSNLMGKIAETVKKKKVIDEETLAEIEEILLYS
                     DTGIEMTEIILQRFREQLKKDKVTDPEIAQIYLTDVMRDILLSEDEEIPDFFAEPSVK
                     PYVIVFVGVNGTGKTTTIGKVAYKFSQMGKKVLIVAGDTFRAAAIEQIAIWAERAGVA
                     IVRSQPDSDPAAIIYDGVNAAMARGYDIVLIDTAGRQHTKDNLMKELSKIDRTIKKLI
                     PEAPHEVILVVDATTGQNAISQAHNFDKSIHLTGIALTKYDGTSKGGIIFNLKHNLKL
                     PVKLLGVGEGIEDLMPFKAVPFVKAFFEE"
     gene            26900..27211
                     /locus_tag="PLB65_02450"
     CDS             26900..27211
                     /locus_tag="PLB65_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HOQ77417.1"
                     /translation="MSNQPPQNQIQIKIDEKVGEGTYANFFVITNSPSEFIMDCGRIL
                     PGIPDARIYTRVLMTPSHAKQLMQLLEKNIDAYENQFGEIKLQGQQDNKPVGFKPDSK
                     I"
     gene            27546..28817
                     /locus_tag="PLB65_02455"
     CDS             27546..28817
                     /locus_tag="PLB65_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOQ77418.1"
                     /translation="MSKIANMLDADLENKICLVRMDHNVVKNGIIKDTMRIDATIPTL
                     MHIFRKGGLPILMSHIGRPFDKKTGTINISEAESVVPVVKYLESKFQLRGLIPDCKPQ
                     DKIGITDYTPIGKAVESLKKGEVDFVYLPNTRWFKGEEAKDESTDVMAKSLAQFADVY
                     INDAFGSWQAHCSTYTIANYLPSYAGLLMQKEISNLQKIFEPKHPFLAIVAGAKFDTK
                     IGPLSSLIKIADKLILGGVIYNAYLCCKYNIKIEGVTNEDIEAANKFISESRDYIHKI
                     VELPLVIENTGMQKRENPKEIDIHILEPGTQLAYVLDVAGSNFAEECVKKVFQEAGTI
                     FVNAVMGYSALFPEGTMAMYSLIDQCKNAVKLFGGGDTIQDFKEYLPGIFAKAQNDPS
                     YYFFTGGGAILDAIQQGSPYGMKPVQALLRD"
     gene            28836..30728
                     /locus_tag="PLB65_02460"
     CDS             28836..30728
                     /locus_tag="PLB65_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOQ77419.1"
                     /translation="MDNDNKLNLLKEFPPPTYEEWLQVVNEGLKGADFEKVMKTQTPE
                     GITLQPIYRKEDVAKLPFMESMPGKSPYVRGNNPIRFLTEGWLIAQNHTDSDLNTLNQ
                     KILKELNQGLTAVNITLANNTSPEGVKVQTLNDWETLLAKIDIKAAPLFMQLGLEDTE
                     ILKTLKQYTDKYNIELKTLQSGIGIDFIGELADRAYLPFSLEETWQKLEETVRWNIEN
                     ASANRIISIDASIYESAGASIVQELSFALSTAICYIKHLQDAGFTIEELAPLFQVKLS
                     LGSNFFMEIAKIRTFRLLWSEMIKAYGGEEEYQKVWIHGKTAKFNKSIYDIYVNVLRT
                     TTEGFAGVIGGVDSLEIGRFNELIAPADELSVRLARNQQIILKEEAHFDKVIDPAGGC
                     YYIEWLTNELANKSWALMQEIEAKGGMLEALINSDIHNYIESVAKQRIEAVNRRKSVF
                     IGVNMYANPLENMVSALPHSNSTCDEFTTTIKPLPKRRAVEDVENLRMQIEASNANKK
                     IFLLNMGTLAEYKARADFALNFFQVASFEVIYPNGFANIDEAIKEAEKSGAKAFCICS
                     TDDNYLNLVPEICSKLKGKIISLAGYPTDKIEDYKKAGINSFIHIKADIVTTLRELAK
                     QMEVIK"
     gene            30725..32863
                     /gene="scpA"
                     /locus_tag="PLB65_02465"
     CDS             30725..32863
                     /gene="scpA"
                     /locus_tag="PLB65_02465"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HOQ77420.1"
                     /translation="MKNPDFLKIKPDFIAEQKTEPKLDFSWKTNEQIEVKPLFFKADM
                     ENIEHLDYLSGLPPFLRGPYPTMYIQRPWTIRQYAGFSTAEESNAFYRRNLAMGQKGL
                     SIAFDLPTHRGYDSDHPRVIGDVGKAGVAIDSILDIKILFDQIPLDKMSVSMTMNGAV
                     IPIMAFYIVAAEEQGVKPEALNGTIQNDILKEYMVRNTYIYPPEPSMRIIADIFSYTS
                     KKMPKFNSISISGYHMHEAGATADLEMAYTLADGLEYVRTALKAGIDIDVIAPRLSFF
                     WAEGMNYFMEVAKLRGARILWAKIIKQFNPKNPKSMALRTHSQTSGWSLTEQDPYNNI
                     TRTCIEALAATMGHTQSLHTNSLDEAIALPTDFSARIARNTQLYLQNETSICRVIDPW
                     AGSYYVEYLTDALMKSAWNHIMEVENLGGMAKAIETGLPKMRIEEAAARRQAKIDSGA
                     DIIVGVNKYPPETEIPIEILEVDNSAVRATQIARLEKLRKERNNEDVEKALEAITKCA
                     ETKNGNLLELAIDAARKRASLGEISYAMEKVWGRHQEAIRLINNVYSSEYAKMDDIEQ
                     VRALTDKFAELEGRRPRILVAKMGQDGHDRGAKVVATAYADMGFDVDVGPLFQTPEET
                     ARQAVENDVHIIGISSLAGGHKTLLPKLMEELKKYGREDIIVTVGGVIPPQDYEFLYQ
                     NGAAAIFGPGTKLPESAKLVMEKLLAHKNE"
     gene            32856..33947
                     /gene="meaB"
                     /locus_tag="PLB65_02470"
     CDS             32856..33947
                     /gene="meaB"
                     /locus_tag="PLB65_02470"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HOQ77421.1"
                     /translation="MNEKRKPEWTPLYSGKEFATSVVTGKKSVHIKASPSTKGKKYDL
                     DTLVKGVLNSERTLLAKAITLIESNAEKHFLQGQELIQKLLPYSGNSIRIGITGVPGA
                     GKSTFIESFGLYLIEQGHKVAVLAIDPSSTISKGSILGDKTRMETLSRNPNSFIRPSP
                     SAGILGGVARKTRETIILCEAAGYDIVLIETVGVGQSETTVRSMVDFFLLMQIAGAGD
                     ELQGIKRGILELADLIVFNKADGDNIQRTEIAKQELQNVLHYMRNATEGWQTNVVTCS
                     AINKTGLDEIWHSISEFYTKTKQNGIFAKRRAQQNTQWFENLITEAVLHRFYQKEEVK
                     TLITQLKDDIAIGKIPIALAVSKILKASE"
     gene            complement(33950..35863)
                     /locus_tag="PLB65_02475"
     CDS             complement(33950..35863)
                     /locus_tag="PLB65_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOQ77422.1"
                     /translation="MQSKQEERGLAYQIIVFIFFFLVLIFSVFIGITRFLFSEIMMDN
                     SRETVSHLAKETVYQIEGRLSAIVDISQSIITLYNSDYLNRNELDTILNDYIYEFDTL
                     ESITIAYAPEYNKSGDSRTIYHFKNKAISQELHPAEYQYLDWFQIPYELKSKCWTDPW
                     YDASSTKQTVISYCVPIFIKGNCIGVMRFDTKLSGLQRIVSPLKLKRSGYAFLVSSIG
                     TIITHPADSISFNESIFSLAEETKDTQLRELGKKMIQGETDFIRLRGNTIFNDSWIYF
                     SPLLSNNWSLGIVIAHKDVVRDLNLLLIIQTLFSVIIFISISLIVYYRTFSVSKPIRL
                     FTEVATKIGSGDFDAQLPPSGNSYEIDHLIHSFAVMQKSLKEYIKNLEITNAEKNRIL
                     AEVQVASEIQRKLIPANTEHPYKVKEIRCYGILEPAGEIGGDLYDFFPVDESHFFFVI
                     ADVAGKGIVASMTMIMVSTYLRTISTYNFTVNEIMKNLNNFLCTQSSAAIFVTALIGI
                     IDLKNDELEFSNAGHTPLFLRKLNGTYEIFADTHSNPLGVFENMEIGSSQLKLDKGDE
                     LILLTDGVTEIMNEKEDFLGIKGVESIIQNLETTNPEQTAKQIMQYVHNFAQSIPQRD
                     DITILVLDYKYPE"
     gene            complement(35853..36416)
                     /locus_tag="PLB65_02480"
     CDS             complement(35853..36416)
                     /locus_tag="PLB65_02480"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ77423.1"
                     /translation="MWKTDDRLLSLIFLQQHKLNMDIVDLDTSINLFLKDGIDIMNVM
                     RYNEYHQLQQAGIDIEDLFTVSFADIGLNVADNGLYTTREFYEKHSKHCRDFVDATIE
                     GWLYAINNQEETISTVLEIMHKHHLPANRPHQKWMLNELSQDFMVDSSQIGVLQKSQF
                     EEAKRLLTEQNMPITSQTYNSFYPYAK"
     gene            complement(36452..36880)
                     /locus_tag="PLB65_02485"
     CDS             complement(36452..36880)
                     /locus_tag="PLB65_02485"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ77424.1"
                     /translation="MNNKRETLALLLLLLSIFLLTGCKKSDKQNGAITQSNMEQSPYS
                     TGLFTIRYLPVWTHQAEFAGVYMAQKKGFYNDKGLNVLIQTGGPHYPPYDNLQDGHSD
                     IVQMSLLTAIKRDSEKDNLVNLAQLMQRTYLMLVGKKPEE"
     gene            complement(37574..39172)
                     /locus_tag="PLB65_02490"
     CDS             complement(37574..39172)
                     /locus_tag="PLB65_02490"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HOQ77425.1"
                     /translation="MAKYIFVMGGVLSSLGKGIATASIGLLLKSMGYKIVLQKFDPYL
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFVDEALTRYSSNSAGQIYESVIQNE
                     RNGVYLGKTVQVIPHVTNEIKNRIQRLDKEYDIIITEVGGTVGDIEGLPFLESVRQLR
                     LDIGYENTLNILLTYVPYIKSAGELKTKPSQHSAYKLREIGIQPDVLLCRSEKPFEEE
                     IYSKIALFTNVKRENVINAIDVDCVYEIPLNFQKAKLPEIICQHFKLEQRPIVMNEWL
                     HFLHNRENAEEVVTIAVCGKYVQHQDAYKSIGEALIHAGASLMKRVKILWIDSEHNFL
                     EEEIATMLNGVQGILVPGGFGVRGIEGKIVFTKYARENNIPYLGICLGMHVAVIECAR
                     DLCNLVDANSTEFNEETLAPVIHLIKDQVYQKRIGGSMRLGAYDCHLDKGSKAYQAYQ
                     TETISERHRHRYEFNNDYREIIQKAGLKISGLSPDNYLVEIVEYPDLDFFIAVQFHPE
                     FKSRPTNPHPLFREFIKAASLKKP"
     gene            complement(39185..40429)
                     /locus_tag="PLB65_02495"
     CDS             complement(39185..40429)
                     /locus_tag="PLB65_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOQ77426.1"
                     /translation="MVDKLKLEKSMALFEEAQTLVPGGVAGIRRPYNFVPGEYPIFFD
                     NGKGGRIFDVDGNEYIDYLCAYGPIIIGYREEEIDNAVIDQIKNKGICFSLTQAVQNA
                     LVKKLRELIPCCDMAALVKTGSDATTIAIRVARGYTGKTKIARCGYHGWHDWCVEVKG
                     GIPQKLYEDIYEFHYNDIDSLEAILKANKDDMAGIIVTPVGHPNGAEVQMPKPGYLEA
                     VRELANQYNCLLIFDEIRSGFRCSLGGAQKLFGITPDLSTIGKAMANGYAIAALVGKE
                     EYMKVLADKVFLSSTFFPNSDGIVAALKTIEILERDKVLDVVAEKGRKFGTEVEKVVA
                     NSGVPVNFTGAPWMPYITFKKDDQGLYKKLRVEFYTQLIRRKVFLQPYHHGYICYRHT
                     DEDLAYTVGAIQESLAEVKKML"
     gene            complement(40668..41864)
                     /locus_tag="PLB65_02500"
     CDS             complement(40668..41864)
                     /locus_tag="PLB65_02500"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HOQ77427.1"
                     /translation="MKQVVVVCAKRSPIGNFGGVFKDISAVQLGVNTLKAMFTETGIK
                     PDMIDEVIIGNVCGAGLGQNVSRQIAIHSGIPETVPAYTVNKVCGSGMKAVSLATLMI
                     STGEADIIVAGGAENMSQIPYALKDARWGARMGSKDMVDLMIRDGLSDIFNDCHMGVT
                     AENLAEKYNISRAEQDAFAASSQNKTEAAQKTDRFKDEIVPVIIPQRKGDPIIVDKDE
                     MPRAGVTVESISKLRPAFKNDGTVTAANASGINDSAAFLILMSADKAKELNLTPLATF
                     VNSASAGVDPSIMGYGPVPAIKKVLQKANWKLSDIELAELNEAFAAQSLAVFKGLELE
                     GIGKLNPEITNVNGGAIALGHPIGASGARIIVTLLHEMKRRNVHKGLSALCIGGGMGI
                     ATLWER"
     gene            complement(42038..42113)
                     /locus_tag="PLB65_02505"
     tRNA            complement(42038..42113)
                     /locus_tag="PLB65_02505"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42078..42080),aa:Gly,seq:ccc)
     gene            complement(42716..43360)
                     /locus_tag="PLB65_02510"
     CDS             complement(42716..43360)
                     /locus_tag="PLB65_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HOQ77428.1"
                     /translation="MLNCAEELILLAIEDKTNTFYKITNINFNVALIGALLMDLALRK
                     RIDVDMEGINILSTEKTGDKFLDTVLENLMNSETGNEPANLVAQLYNSMGHLKNKLLQ
                     DLEDKGILKVSQCKVFWLFKQRRYPVIDQKEEEEVLSRIRKTVLTDIEPEPRDLALIA
                     LLNICNLMDKIFTQEELEQRHNRLEQLKKMDLISPAVKRIIGEMQVLITTSYTT"
     gene            complement(43392..44681)
                     /locus_tag="PLB65_02515"
     CDS             complement(43392..44681)
                     /locus_tag="PLB65_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ77429.1"
                     /translation="MRKLFILAITIFVLLAGCKSALQSEKVKITFWHGLSGPLGDTLN
                     EMIREFNRTHKNVEVITNPISSYTALSQKLMASIQAKKQPDIAQVFESWTSKYIEAGV
                     LCNFEELMRADTTWTKEDLEDIYPVFLKSNTFNDTIYSFPFNKSVRAYFYNKDAFYRA
                     GLDPNHFPATWNEFRLYCRKLTTDTNNDGIPETYGTNFNVNEWQFINLLYQAGGAILD
                     ELGNPQLNSPQGIEALTFITDLLNKDKTVYLVREFEGQNDFLAGIAAMYEGSSVSITH
                     MRQQPINFNIGYAPLPTYRTNKSAVSGTNIVIFKSGDKKREQAAWEFIKWFTDTNQTA
                     RWSADTCYMPVRKSAMQSAILKNFLKDYPQFQGIYNQLETAVFEPQIPAWFKARLELK
                     GYLEKAMRGISTPKEALDEMNEKFKSIIYEEQYIKNL"
     gene            complement(45003..45716)
                     /locus_tag="PLB65_02520"
     CDS             complement(45003..45716)
                     /locus_tag="PLB65_02520"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HOQ77430.1"
                     /translation="MLKLTPNTKVVVLTGAGISAESGIRTFRDSDGLWEDHRVEDVAT
                     PEAFFSNPVMVWNFYRERYNDSLSRIPNPAHLALVKLENFLGDNFLLVTQNIDRLHHR
                     AGSQRVLEMHGSLSNCLCTKCQSKLAIKDIDLNKAIPLCPYCQGFLRPDIVWFGEFPH
                     YLDMIDDALRECSVLIVIGTSGVVYPAAGFVITAKLMGARTVAINLEKPDNLGYIDEF
                     YQGKSGELLPKLVDMWISG"
     gene            complement(45722..49306)
                     /locus_tag="PLB65_02525"
     CDS             complement(45722..49306)
                     /locus_tag="PLB65_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ77431.1"
                     /translation="MKNIALLVLLVFSTAVLIAGVSVNYSLEMPKIDSNGQYTKIKLE
                     NTQSWGQPGNPDLPWFGIKILLPEGMEADKIVVSKTDPVVYSLPKRVSPIQKQYPFSH
                     KIIETPDAPNLEVYNSSAIYPDNPDNGVRTEFLCGHPIAMSAISPFEYCPLKNELVFY
                     RNLSVDISYSTSSKAMDAMRFLKNDAYTVNRLQKAVDNPETIIMNQTRDAGVEYLIVI
                     DASKEMQWTPFKTFQEARGRNVLMKTMTDITNNTIGIDTQDKLRNYIISLYTTNPLRY
                     VFLAGDTDVIPHRGFYVSVSDGDEYTDADIPADMYYACLDGTWNDDGDSYWGEMYETD
                     LAPELAIGRFCYNDDSEITNFLNKLNSYTNNPITNGIKTALFVGEWLWDGPTWGGDYM
                     DEMIGSSSNNGYTTTGVPASWNISTLYDRTYGAADSWGPSQIRPLLSQGPNLVNHLGH
                     SSTTYTMRLSNNQVNASTITNDGINYNFSTYFTQGCYAGAFDNRETNVGQYTSDSITE
                     KMTSIATGPVAMISHSRYGWGMQGSTDGASQYIHRQFIDAIFGENYQEVGYALVDSKI
                     DNIPFITNEPVMYWVTYETNLFGDPSMMIWTNTPQTITADLPANLLVGVNNYQIQTNA
                     PNAEFIMKYEGSTIYKTISDASGLIQINLFATLTPGLYNIYINAPNFYPFNTTITATA
                     EQMPYIVCNHISFNDQDGLYHSGDTLTMDFYIKNVGLLDLTTPGTLTLLSNSPNIQIT
                     SSTANFDGISVGDSLAINAAFQFNVIGNYPDHTNAMLQIRANYDSFTSTSIAYITLNA
                     PIIDLTSYQIHNSSLIIMPGDSPTINFSISNTGSGNAYNPILILFSSYSDIILDQYEV
                     VLDPIYANTTLNFQNVISFHILDSASIDDNYTIDYIFSADNGNVIEGSFLVHLGAISY
                     SFETNYQDWETVALSSNFINQWHRSSTRNHTPNGTYAMKFGGNGSGQYAGSTYGALIS
                     PVMNVNPNCQLKFYHWMDAEKHSTNQSYAWDGGLVEMSLNGGDWTQITPVGGYPYKIY
                     NNSASPFPANTWVYSGTINWSEAIFELGNISGTAQFRWIFGSDAYVGGEGWYIDDVNI
                     TGIVDNIDETTPSLENVVLHNNYPNPFNPETTIEFQLPSKMPICLEVYNSKGQLVNRL
                     IDDIMDAGIHKVVFTGLDMHKHNIGSGVYYYRLRTPSGTVTHKMLLIK"
     gene            49634..50626
                     /locus_tag="PLB65_02530"
     CDS             49634..50626
                     /locus_tag="PLB65_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase"
                     /protein_id="HOQ77432.1"
                     /translation="MKIHKAIALFSGGLDSIIAVKWMQNRGYSVHSVFFKAPYIFPER
                     ALHYASKNNIELEVIDISEDHIKLLDNPVYGFGKYFNPCIDCHGLMFKKAAELLPSRN
                     ADYIISGEVLGQRPKSQRHDALDSVGRLSGVKDLIIRPLSQNLLSDTLPLREGWIDKQ
                     DMLSIHGRGRYAQLALAEQLGIVDFPSPAGGCLLTDRNYCLRIKDLKKHQQDTLENIE
                     LLAWGRHFRLDNETKLIIGRDEAENEVLENMFRNGTKLQARDYMGPVGFITGNASYET
                     LSLALSIFLLYHPKAPENAAIEIWKVIDKQLTQHTEKIATKAKKELNKDYLISY"
     gene            50643..51269
                     /locus_tag="PLB65_02535"
     CDS             50643..51269
                     /locus_tag="PLB65_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ77433.1"
                     /translation="MLKIESTVLLESYQTNTYLLWETDSSNAFLIDPANPSEAFLKRI
                     RQLELKVKMIINTHGHADHIGGNHFFAKALNCPVAIHSLDAEMLTDNSKNFSIYMGFE
                     LSLFGPQVILNDGDELFLGTDKVKVIHTPGHTKGSICLLADKYLISGDTLFEQSIGRT
                     DFPGGSHTAIIDSIKNKLFILDDEVLVFPGHGFTTSIGLEKKNNPFIY"
     gene            51278..52813
                     /gene="guaA"
                     /locus_tag="PLB65_02540"
     CDS             51278..52813
                     /gene="guaA"
                     /locus_tag="PLB65_02540"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOQ77434.1"
                     /translation="MHNKVLILDFGSQYTQLIARKIRSLNIYCEIHPFTISADKLYSF
                     APKAIILSGSPYSINNNETPKPDECIWQLGIPILGICYGMQLMVAHFGGKVQPSAKRE
                     YGLAYVKIIDHNELLADLGNREEVWMSHSDAISNLPECFSVLAETENSPYAAIAHNEL
                     PFYALQFHPEVVHTKNGIKILENFLYRIVNLTPDWTAGTFINKSIEKIRSQVGDKKVI
                     LGLSGGVDSTVTAALLYRAIGKQLLPIFVDTGLMRYREADRVKETFSAYPDLNIRFVD
                     AGETFLNKLKGITDPETKRKIIGGTFIEVFDKVASEFEDVHFLAQGTLYPDVIESASL
                     TGPSATIKSHHNVGGLPDNMLLVLIEPLRELFKDEVREVGTLLGLPESIVQRHPFPGP
                     GLAVRILGEVDAQKVNILQLADEIFISELQENGLYNKTWQAFVVLLPVRTVGVMGDER
                     SYEQVCALRAVDSVDGMTATFSQLPFHFLQKVANRICNEVKGISRVVYDLSSKPPATI
                     EWE"
BASE COUNT        17306 a         9277 c         9793 g        16614 t
ORIGIN      
        1 ttataaatag aatataaaca agattatcag gattcaatta ttggaaatag atatttgatt
       61 aattggctag cccattaacc tccactgcat tattgggatt aatgtcttga tttatccttg
      121 ctcgttaacg ctatgcgggt gattagattc ccatataact cccttatccg atatgggaac
      181 gataagggaa tcttatggga aatataccgg ttggacaagt ggagcgagta agcaacaaaa
      241 agcccagcaa atagccgggc ttaagtataa atgtaggtta aattaattgt ttgtttattt
      301 ctcggcagtc actttgaaga agcgtttaga gccgagttca gacaaattca gaataagttg
      361 tcggttggat gcaggcatgg tttgaaagag aacaaaagtt cctaaaggat cttcactggc
      421 gtaaatttta taattggtaa tggttatggg agtttgagat acagaaagtg taacggcgtc
      481 ccagctaagg agatagctgt tctcaacgga ttgtaaaact acattttggg gagcaagagg
      541 tgtttcgtca attacataaa gttggaaaac atcgctgctg tctattagag aaccggaaat
      601 cataattaac cggatagcgt aattacccca ggtattaaaa acggtatgca aatcccattc
      661 gctggaaaca ggtaaaccgt taacgagcca gatagggtga ctttcgggag tattatgccc
      721 aatagaatta tttataaact gaagaggatg tcccacaaag aaaggacctt ccggagttat
      781 aatagaggcg ctatgtgaat aatggtaaca accaatatcg gcaatgctgc catccgggtc
      841 aggagttgta accggactgc cagcatcaat acaaggactg gtgtaaagca aatgaaagtc
      901 atgctctatg gcattggcaa aatggggatt agcattgata ttacctattc cgggatagac
      961 catagtgcca tagtttacat cattgtaaac agcatttaca gttccttctt ggcaaacgaa
     1021 aggaacgctg ataggggtat tgctccaagc gatattattg ctaaagttta gctgagcgga
     1081 attaaccgtg gcaatatttg ggtaaccata aatggtgtta ttttggaaag taaaagccgg
     1141 aatagcatta atggataatg ccagataatt ctgcaggtct ggaggggaaa tttgaagatc
     1201 atccactaca aaaagattgc gttcaatata attaaaaacc gagctgaaat tttgcaatcc
     1261 ggttttgcaa ttcaaaatca tagaattctg catatccaaa tttcctccgc tattaatccg
     1321 gatacctatt tcggcatttt cgattttagc gtctcttaaa ataggatttg cattaccgtt
     1381 aatcaaaatc ccggtgccat aatgtgatat ttccacatta ctaatgttgg cagcggtatt
     1441 gcctgataga gtgattcccg ttccgttgat taaaacagtg gcatcgatgg gcgctatctg
     1501 gacagaatgg atgataggat tgcaatttag aactgtaatc ggatttaggg cattcaggat
     1561 ttcacaattg gtaaaattgc tttcttgccc aatacccgtg gttctggaaa ttgctaaacc
     1621 actccaaagc aaatcggaaa aaccaggcat aaaagttaca ttttgagcgg aaatagagcc
     1681 ataaacattg atcggttcat cattccagat ttgaactatc actcctggtt cgatagtgat
     1741 atgtttagtg gaagctatta tgattggttc cacaatatga tagggagaat tttctgaagt
     1801 ccagttggta ggtaaattat ctttatctac aatgaaccca tcggagatat ttatcagtac
     1861 atttactaag acagaatcgc tgctttggca gataccgtca aacacattga aagtaattgt
     1921 ttcggttcca aaccaattat gagttgcgct gaggttaacc tgcgagccat caatagttgc
     1981 tataacatta ttggcttcgc tacaggtaat aattaagggg tcattatccg catcgctcac
     2041 ataagtagaa aaatccactg ttaatgtttc atttacatca aaagaaaagc tcgcaggtaa
     2101 attaattaca ggaggatgat taaccggcaa aacagtcaga aaaacattaa gtatagtaag
     2161 cggattagta ggatcattgg aattgacaag taactgggca ttatacaatc cagcaactaa
     2221 ttcctgcgct gaaaaagtaa catttattgt agtagttccg cttacgggaa tatctccttg
     2281 gcttggttca atggaaagcc aggggatagt atgaatgatt ttgtttagga ttagttgtcc
     2341 atttagaatg tgtggttcac caccataaac atcaccttga attgtatagg gcaaaataag
     2401 gtttccatta aaggtaagag gaattgttat gtttatattg gcagtagcag tttgacctcc
     2461 ttgaataact ccataaccat tagaactctc accatgccaa tgaattgtaa ttccgtcacc
     2521 agaacttatg ttggggagca tatcatccgt accacctgta aaattggagg cagaattaac
     2581 tataatatca gcagggaaag taagatacac atccttcaac cattctaaat cggagctgtt
     2641 attatagaga gtaaatgtcc aattaatagt tgtcccaggt tcataatatg cgtaatttaa
     2701 agttaaagtg ctgccttgga tgcttttatc ttccggtagc ttattttgag taaaacggct
     2761 acgcgtttcg ggatagatat aataagataa aggtaatcca ccggtattag atatagtgaa
     2821 agtttctgta gaatcggtct gagcatatac agtaaccgct aattcattta tgctaacagc
     2881 aatctcaggg ggaatataaa cgctaccgga aacttgaatg ttaacaagag gattgtttgt
     2941 agcattactg gaaattgtta caaccccatc aaaacttcct gctgaagggg aagagaaaat
     3001 taacgcataa gttttggtgg ccccggcaac gatggcaata tctaatatat tgcggctaga
     3061 agcattactt tctgaacttt gtaaattatc tcttgtgctt tcggaaacag tgtagccagc
     3121 aggagtaaaa attgttccat ttagagtttg atctccataa ttttggatag taaattctcg
     3181 agtttggctt tcgcctatgg gtatactgcc aaaatttaaa gaattgggag ttacagtaat
     3241 ttcaggacca gtgtaatttg gggcaacctt caacttaata ttggcgcgtg tggaagatga
     3301 acttccgcct gaaaaaatat tggtctgatt agaattatca ttaacaagat aatggtaacc
     3361 attatcaata gaagtgtatt gaacggttcc agagctatta taagagctgg caatgccaaa
     3421 ggcagtatcc accaaaagat tatccgttcc attccataga aatggcgtgg tgaaattata
     3481 catattccaa ccggtctgag taggtaaata acttggattg gaatatactg taactaaatt
     3541 atctgcagtg acaaacgaag atacattagt agccgtagta tgcttcaacc tcacaacaaa
     3601 gttaggcatc gtaaccgttg gcagactaac tatgttaaag ccaagctgag taatatttat
     3661 agggccaaag atacctgcag cgtttaattc tgcttttgta tagacggatt gaccgtgtaa
     3721 acttttatac catacattaa tcggagaagg ggcagaattt cccgtaacgg aagttcctgt
     3781 gccaataatg gcttccgaaa caggttgaat taaagatatg gagatttcac taattgtttc
     3841 attattacta ttatctttag ccactgcttt aatagtatag cttccagcag ttaaaccagt
     3901 agtattccaa ttataagaat aaggatctga ataatcagta ttgtaaagta tgttattgat
     3961 gtaggtttta acggaagcaa tagagccatt actgtctgta gcgtttacat taatagtgat
     4021 gatagaattg agagcaaaac tggctccgtc tgcaggagaa gtaatttggc aagtgggcat
     4081 tccacttaca atagtattta aagcggcatt ggcatttacc attccgtagc cggtatatct
     4141 atcccaacca acacctccat caattgtcat atcggttgcg gtagaagtaa gcttagaaaa
     4201 cacttgcgcg ggggttaaag aaggatcttt agaaagaagt aaagctgcca ctccagcagc
     4261 ataaggtgtg gcacaagaag ttccattaaa ccatagataa taatctccgt tggtattata
     4321 gccatttcct gttcccgtta aatcggtggc aggtaaaatt gtcggtgcca ttatgtccac
     4381 ggcattttta ttgttctgag tattaactcc gtaattggaa ccccaccaat attctccatc
     4441 ggaagaagtg gtactttttc tctgtcctga aggactggca gcgcctacgc ttattacaga
     4501 agtttcattg gcgggataag aaatagtgga attattttcg tttccggtgg cggcaaataa
     4561 tactactcca gaattatagg cgtaatttaa agctgcgtcc gtagaaggtg aatctccttc
     4621 tattatatcc gttgccccca aacttatact ggcaatatga gcattatgat cagcggcata
     4681 agttaaagca ttttcaatgg cagtaaaata attgactcct tcagaattgg ctacttttaa
     4741 aggcataata ctgcagccac cggcaattcc tgcaataccc aaagagttat ttgcttttcc
     4801 agctgcaatt ccagaacagg cagttccatg tcccggatct gaactgttat ccataggatt
     4861 gctgtcatta tcgccaaaat cataacccgt cactaagcgc aaatcgggat gggcagtatc
     4921 aacgcctgaa tcaataatgg caattatgat gctggctgaa ccataaccct ggctttgatt
     4981 ccaagcaagc tgggcatcac tgtcaaaacc gatagtgcca actcctgagc cggtatggcc
     5041 acttgaagta taagcaggta actgagctgt gttattatga ccccaattat tggcgtagta
     5101 agtatcgttt ggaactactg taggataggc aatatattcg ggaattgctt cttctacata
     5161 acgattggct atgaattgtt taagagcttc ttccacggtt gttttgccat tcaatttcaa
     5221 gataaaccaa cgatcaaagc ccgtctgctt ttcccaattt gcgtcattta ctttgcgatg
     5281 agctttgatt attttagtgg ctcctgtttg agccatcagc tgatccagtt catttattcc
     5341 cgtattggtt ttttcagcat aaagatctgt agggagattt gcccggtaaa ctacttcggc
     5401 gcctagttta attttaataa gatcagatgc atatgagggt ttgttatcga tcttactatt
     5461 caaaggagca taggcaaata aagaaataat aagtaaaagc atcaacatag ctataaactg
     5521 tcttttcatt ttttctccag ataaaaaata ctattactac aatataatgg ataatgcagt
     5581 ttaaaccaac gattttttct ctcataagtt caagagtata aatcccctat taatttgtag
     5641 tcgtttttta gtttgtttcc agagtgatag tggagaaaaa aaacacttga ccaaattggt
     5701 atttccaaag aaaagggaaa aaacctttaa tcacagccag tgagctggaa aggagaaaga
     5761 atgacttcaa aaccacaatt tactggacgc tctctgtttg acttagatga ctattcccat
     5821 gaagaaatta tgtatattct ggaagcagcc aaaggaatga aagaaattaa cttacgggaa
     5881 tacaaaaaaa tacctacact cagaggtaaa actatttgta ctctttttgt agaaaacagc
     5941 acccgcacaa gaatgagttt tgaactggct gccaatcgtt taagtgcaga tgtagtaagt
     6001 tttcaatcgt ccatatcttc tttacaaaaa ggagaaagtt tacaagatac tgtctacact
     6061 cttaatgcaa tgggtataga tctttactgc attcgtcata gcagtcctgg aagcccgcaa
     6121 ttggttaata aatattcagg aaaacctgta attaatggag gtgatggtag acattcccat
     6181 cccacgcagg cacttcttga tatcttttca atttgggaaa aattgggaga ccttaaagga
     6241 ctgaaaatta ctattgtagg cgatatttta aatagtcgcg tagttcgtag taatttaatt
     6301 ggaatgggca aactgggtgc taaagtaaca gtatgcggtc ctaaaactct aatgccaggt
     6361 aatatggaaa gcgtttatga ttgccgcgtt gaatatgatt tgccaagagc attaaaagat
     6421 gctgatgtag ttatgggctt aagaatgcag ctggaaagga tgacagaagg gttatttcct
     6481 tctttggaag aatacagtaa acactatgtc ctttccaaag aaaccttaaa atatgctaaa
     6541 cgaaatgctc ttattatgca tccagggcca atgaataggg gagtggaaat ccttcctgaa
     6601 attgccgata gcaaccaaag tgtaatcgtg gaacaagttg ctaatggagt tgctgttaga
     6661 atggcattaa tgtttttaat cttaggcgga aaggcataag gagaaatatt atgaagatct
     6721 taattaaaaa tgcaaaaatc ttccaaaagg ataaattcat aaaaggtgat gtgctaatag
     6781 aacggaaaaa gattaccaaa ttgggaggca ctataacagt taatgccgat aaaattatcg
     6841 atgcccaagg tgcctgtgtt tttccaggtt ttattgatct tcattctcat ttacgagatc
     6901 ctggccatac ttacaaggaa gatattgtta caggaactaa agctgctgct catggaggtt
     6961 ttaccactgt ttgcgctatg ccaaatactg aaccagttac cgataatatt gcctctgtgg
     7021 aatatataca acttcgtgcc aaagattatg gctctgccag ggtatatgta attggagcta
     7081 ttacaaagca atctgcaggc gaagaaattg cagaaatggc aactatgaaa tctggaggta
     7141 ttgtagctgt atccgatgac ggtaaatgtg ttcaggatgc gcgtttgatg ctctcttgta
     7201 tgaaatatgc tagtaatttc ggactgccca ttattattca tcccgaggat tattctttgg
     7261 ctggaaaggg acaaatccat tccggaaaag ttgctaccaa gcttggtctt agtggtattc
     7321 cgggtttggc tgaagaagtt attattgcca gggatattat gcttgctgaa agtgctgggg
     7381 cacaacttca tattgctcat atttccactg cccgatcttt ggaattagta aaaaatgcca
     7441 aagataaagg attaccggta acctgtgaag taactcctca ccacttagtg ttaaatgaag
     7501 aagctacgat aacattcaat acaaatacca aaatgaaacc acctctaaga agtgaaaaag
     7561 atcgcctggc ttgtgttcag gcattaaagg atggtctgat tgattgtatt gctacagacc
     7621 atgctcctca tgcagatttt gaaaaagaaa aggaatatga tcatgctccc tttggaatca
     7681 tcggattaga aacagctttt ccagtgctct ataaaaatct ggtacaaaca ggactgataa
     7741 gtttaaatcg tttagtagaa gcattaacaa tagctcctgc taaaatatta gatttacctg
     7801 gtggtgtact ggaagtaggt aaagcagctg atttaaccat tatagattta gagaaggaaa
     7861 ccctcttctc ggtagaaaat attctatcta aaagtaaaaa tactccttgg ctaaatcaat
     7921 ttttaccggg aagagtgtta tgcacaatct gcaacggcaa tgtaacctat atggatagtg
     7981 aaaaatggac aaattgaaac cccaattttt agaacgagca atatactctc gtgaagaact
     8041 ctgcggagat tatttcatac tgtggatatg ggatgaagag ctggggaaaa aatgccaacc
     8101 aggtcaattt tttgaaatac gcgctcatta cagtattgaa aatgcagaag aaacacgttc
     8161 gttacccaag ctttttaaac caatctctat ctatgataat gctgaaggta ggattggatt
     8221 ctttattaaa aaagtgggat acggcacaaa tgctcttagt aaactgaaag ctggagatct
     8281 acttgaattg atcggtcctc taggaaatgg ctttcctata gttagcggaa aaaaaatact
     8341 attagtaagc ggtggaattg gttatccacc tctatggtat cttcaaaaaa gacttattaa
     8401 acacaatcta atttattggg ttcatggggg gtcgaatcaa tgtgatgttt tcccttgtga
     8461 tgaggtctgg actgaagatg gcagcttagg taaaatgggg atggttactg aaggagtaag
     8521 atctcttatt atggagaaga agataaattt aatatacagc tgtggaccaa tcccgatgct
     8581 gaaggaatgt gccagaatag ctttagaact tgaaattgaa cactactgtt ctttggaagc
     8641 atatatggca tgcggaattg gtgtctgtta tggttgtgct gttcctatcg gtaatactga
     8701 agattggaat tatttaagag tctgtaaaga aggtcctgtt tttaatgcta aggaggtttt
     8761 gtggaacaga atgtaagcca aatttatact tcgttgggac gcttaacatt aaatagtcca
     8821 gtaactattg cttccgggac ttttgctctg gagaatctcg attttatggc gcaggactat
     8881 ttaggtgctt atgtatgtaa aactattacg cgttacccta aaaaaggaaa tcctccacct
     8941 cgtctgtttg aaacagaagc aggattgcta aattccattg gattacaaaa tccgggactg
     9001 gataaattca ttaatgatga tttacctata cttagagaaa cacttactac tcccttaatt
     9061 gttagttttt ctggctcttc cctaaatgaa ttcaaattaa tgttggaatc tttggaacag
     9121 aatgaaggaa tagatggtta tgaactaaat gtttcctgtc ccaatgttga aaatgagggt
     9181 attgcctttg gagttgatcc tgaaattgtc tataagcttt gtgcacttct tgcgccgtta
     9241 actaataggg aattaattat taagcttagc ccaaatgtaa ccgatattgc cgttattgct
     9301 aaagctgctg aagatggagg agcaacatct atctcgttaa taaatacaat ttggggaatg
     9361 gcaattgata tccaaagcgg aaaatcgatg ttaaaaaaag gaattggcgg gtacagcgga
     9421 attgggataa aacccttagc tctagcttta acttatcgag cagcaaaagc tgtagatata
     9481 cctataattg ctatgggtgg aatttatagc tggcaggatg ctctggaatt tttttgggct
     9541 ggtgcttcag ctgttgctct ggggactgca aattttatcg atccactagc tgttcctaaa
     9601 gtatatgaag gtatagataa tttcttgcga gaaagaaatt gtactctaaa tgagataata
     9661 ggtaagtgcg aagtataagt aaaataatga gcagatataa tgaatattat cgatattgct
     9721 aaaattagaa ccaatgtaag ccttaaaagc tgcaataatc tatgccttct aataagcata
     9781 catcttgggc aaaataacaa ttgacttaaa taccaagtaa aaaaaaatgg ctcattactt
     9841 tagaattata cataaaaaat aaggaataaa cttactgagg aagaaatgaa agaatatgaa
     9901 atgaaaaaga tccgtaatct attgttcatt ggtgccagtg gtgcaggtaa gacaactttg
     9961 gctgaacaaa tcttctattt gactaaaaca agcaaccgtc tgggcaaaat tgatgaaggc
    10021 aatactataa tggattttga tcccgaagaa ataactaaac aaatgtcttt ggggctttcc
    10081 attggttatg ttaattattt agaccataaa attaacattc tggacgcacc aggctgtcct
    10141 gattttgttg gagatacaat agtagctctt ccagcagtgg aaaatgttgt tatagtagcc
    10201 aatgctgcag gtggttatga agttggttta gaacttgcca tagaacagat agaaaaaagc
    10261 aaaatgggta aagtaattgt tgttaataga atagacaatg aacattcaga ttatgataaa
    10321 acccttgaag caatagcaga aaatactaaa atcaaacccg taaaagtcca tattccgatt
    10381 ggtaaagaag gcagcttcga gggtgtgata gatattataa agcgcaaagc tatcaaaggt
    10441 aaaaccgaaa gtgatatccc ggccaatatg agtgaaatag tagaagaagc cagaatgcac
    10501 ttgatggaag cagtcgcaga aactgatgag gatttactta atgattatct ggaaaatatg
    10561 gagcttcctg aagataaact aatagcgggg cttaaaaaag gtatcatcag cggtgaaata
    10621 tctcctgctt ttgtttgctc tgcaggaacc ggtgaaggtg tgcttgcttt attaagtgga
    10681 attatcgact atcttccttc tcctgaagat tccaatgaaa tggaagtttt aaaagaaaac
    10741 gaaaaagtta gctttatctg taaacccgat ggtgaacttt taggctattt atttaagctt
    10801 tatgctgatc ccagtatggg tgattttgcc tatgcacgca tattctctgg aactctaaaa
    10861 acaggtgtgg aattttacat tcccgaaaaa gatgcaaaag ataaagcagg aactatgtat
    10921 tatatgctgg gcaagaatcg taatgattgc aacgaaatta aagctggcga tattggcgct
    10981 ctgttaaagc tgaaaaatgc aaaggttatg aatagtatcg tggccaccgg ttcaaagttg
    11041 tctgtggtgc ctgttgaatt acctactcca actacctggc aaaccttgaa agcagtgaat
    11101 caagctgatg aagataaaat cggaactgtt ctgcaaagag ttattgctga agatcctact
    11161 attaattatg agcttaatgt ggagacccat gaaaatgtac tttccggtat gggtgaacag
    11221 caattgcaat tagtattaaa gaaactgaaa aaccgctata aagtagatgc tatccttaag
    11281 gatcctcgta ttccttataa ggaaacaatt atggcaagtt cagaatctaa ttataaacac
    11341 aaaaagcaat caggtggtcg cggacagtat gctgatgtct attttcgcat aaagccaacc
    11401 gaaaggggag aaggatttaa atttatcaat tccattgtcg gtggtgttat cccttccaat
    11461 tatattcctg ctatcgaaaa aggtcttatt gaaacaatgg aaaaaggaat tatagctgga
    11521 tacccggttg tagatatttc tgttgaagtg tattatggta gctaccacga agtggacagt
    11581 tctgaaattg ctttcaaaat agcctcttca atggctttaa aagaaggatt caaaaaaagc
    11641 aaacctatat tattggaacc cattaatgag ctggttatta ttgttcccag tgaatatatg
    11701 ggagatgtta tgggtgatat ttctactcgc agaggtaaaa ttctgggtat ggaacaaaac
    11761 ggtaaaaagc agtatttaac agctcaaatg cctttagcag aaatgtattc atattatcct
    11821 gctttgaaat cctttactca gggacggggc cgctttacgc agaaattctc tcattacgag
    11881 aaagttcctg atgatattgc acagaaagtt atagctgcct ggcaggaaca gtcaaacgaa
    11941 taatacgatt taacaggtga gaaccctaag ttaaatcaca atagatataa gaggaacggc
    12001 gttcgcgtcg ttccctgttt tagaaattaa aaaagctatc tctatttttc atagagcaat
    12061 aaattaatct ggtttaccga ttttttcttt tttacttgac agtagaagat aggtttacat
    12121 agtgttctta agtagtgata aataaaaaaa cgaggttaat atgtctggac ataataagtg
    12181 gagttcaatt aaacataaaa aaggtgctgc cgatgcaaag agagggcaga tttttacccg
    12241 tttagttaag gaaataattt tagcagcaag agctggcggt ggcgatcctg atatgaatcc
    12301 tcgcttacga actgctatta atacagctaa agaaaacaat atgcccaagg aaaatatcga
    12361 acgcgctatt aaaagaggca caggtgaaat tgaaggtgca gcttatgaag agattattta
    12421 tgaaggttat gggcatagtg gagtgggaat agtcgttgaa gtgatgacgg ataacaaaaa
    12481 acgaagtgtt tctgaattgc ggcacatatt ttcaaaatat ggtggaaacc ttgccgagac
    12541 aggatcagtt gcttggaatt ttgaacagaa gggttttatg aatgttcctg cagcaggatt
    12601 ggatgaggat gattttatga tgaaggcact ggaagcagga gctgaagatg tagagctaaa
    12661 tgaaaataac tttgatatat acacttcccc tacggatttt cacacagtaa tcagcaattt
    12721 agaaaaggag ggtttcccta tccataatgc agaattaacc agggttccga aaaatactat
    12781 agcggttgat gacgacatag ctgaaaagct tatgcgtttg attgattccc tcgaagaact
    12841 cgatgatgtg caaaaagttt atgccaattt tgagttttcc gatgccttta tggagaaact
    12901 ttcccaagca cagtaattaa gtgattatca ttggtttaga tcccggcagc cgttattgcg
    12961 gttatggtct tcttcaaatt gaagacagaa aaattcttgc tgcaggttgt gatgtgctgg
    13021 atgtaacaaa aggtaaaact ctttcgcttc gtttaaaatc actttatgag aatattatag
    13081 cagttttgga tgaatatcaa cctgaaatag cggccgtaga aagcattttt tttcatcgtc
    13141 aggtgagaag tgtttttaca ctcggtcatg ccaggggtgt aatattactt gccttagcca
    13201 tgaaagatat accaattgta gaatatagtc cccgcgaaat aaaaaaatct gtttgcggga
    13261 atggtagtgc tagtaaacaa caagttcgct atatgataaa taagatttat cccttaaaag
    13321 aaagtcctaa aagggatgat gcttttgatg ctttagcggt agcgacctgt tattacaaca
    13381 ggatgaagtg gctgtaatga ttcaatatat ttcaggtact cttactttca aaaatcctgt
    13441 aatggctatt attgaaacta tgggtattgg ttgggaaata aagattccga ttagtactta
    13501 tgaagtgtta ccttctgttg gaaagaattg taagctatta acttttttac atatcagcca
    13561 ggatgatatg cgtatttttg gtttttctac agaagcagag agagagctat ttatgatgtt
    13621 gaataaagtt agtggcatag ggccgaaaat agccctttca attctttcaa ctttaagtat
    13681 tcctacattt ataaaagcga ttaattcagg tgaagaggga ttacttacaa gagttcctgg
    13741 cattgggaaa aaatctgcgc aacgtttaat tgtagagttg caagatgatg ttcacacttt
    13801 aatgagtcat attgaccaga aagatttaca aattggagat aaaaatagtg aagttgaatc
    13861 agcattgctt tctttggggt tcaatttaca gcaaatcaga aaagaacttg ctcttcttga
    13921 tgaatcgatg aggcagttga acacagaaac tttaattaaa gaaattatca agcgtattta
    13981 tcagagaaat aaatgaattt tcgtaccgaa gcatattcta ctatcctaaa agtgctaaaa
    14041 gataaagagt attctgatac tctacttcat cagcgcgcaa aaaaaataaa gaatgctgga
    14101 gaagatcctg ctttatttta caacctcgtg aaaggagtta tcaagctctg tttgaagttg
    14161 gattatatcc tgcagcaata tgtggataaa aagaagtttg cttctacaga tgtaaaaatt
    14221 aaaacccttc tttatatggg tttatatcag cttatgtatc taaattccat tccagatcat
    14281 gcagctgtga atgaaacagt tgaattagcc aaaactctgt ttaatgaaca agtagcagat
    14341 tttgtaaatg ctgttttgag aagttatctt cgcaatcctg aaattaccta tcccaaagaa
    14401 cctgaattga ggattgccta tgaacattct tttcctccag aattggttaa acagtggctg
    14461 gaattatggg gcgaggaaaa tactgaatat ctggctatgt tttttaacga aaatcctgaa
    14521 ctgcatatac gagtaaatac aacagctaca acacctgata aattgaaagc atattttgct
    14581 aaacgggata ttatattaaa accttctgca gcaagctcta atatgtttta tacagctcaa
    14641 acagcagagg ttctggagga tgtagctttc tctgaaggtt atttttctgt tcaagatact
    14701 tctgcggcat taattgtaga attgcttgat cctcaaaaag aagaaagtgt tcttgattta
    14761 tttgctgctc ccggaggaaa atgcagttat attgcagaaa gaatgaataa tacaggagaa
    14821 gttattgctg tagataaaat accaagtaag atgaaactat taaaacaagc agctgaccgt
    14881 ttgcagctta ctaatatcaa aacggtaatt acagatgctt tcaaatatgg tcctgtagct
    14941 cctgcttttc aaagagtgtt agtagatgca ccttgttctg gatggggtgt ttttggcaga
    15001 aaatctgatc tacgctggca ggttcatcag aatattcccg agcttatcaa gctgcaagaa
    15061 aaagctttgg attatgcagc taattttgtt gctccgaacg ggtttttggt ttattctaca
    15121 tgcacaatga acccgaagga aaacgaggac caaattgcct cttttttaaa aagaaataaa
    15181 aagtttgaac tggttccagc ttctcagttt attcctgata agtataccag ttccggtttt
    15241 ttaaaaacga taccttttaa gcataatatg gatggtgcat ttgccgcaaa attacaacgg
    15301 attaaataag aatagatacc tgcaaggcaa aggaaaaacc aagaataagc aaatcggcta
    15361 ccttgtagga attgggattt gtgtaatcat tgttactgcc tttgttttca gccaaatctt
    15421 ttttcctata attttgggaa gaacacctaa agtagaagta ccctctgtag tcgggatgaa
    15481 ccttgctcat gctaaaaaaa tattgcagga tgaaaaattg catgctgtag ttaaagattc
    15541 tttatttagc gatgttgcag ctcctgaggt tgttttagaa cagcatcctg tccctggaga
    15601 taaattacat caagatggaa cagtatattt agtaattagt aaaggatcat caacagtcac
    15661 agttccttct gttgttggaa agccttttca ggaagcattc ataattttaa gaaatcttga
    15721 tttgcatagc actgtagtgg attctaccta ttctgatatc tatcctgtaa atactgttat
    15781 tcgtagcgtt ccttcagctg gagcaaaagc tctaaaaaaa tctacagtaa agctcatttt
    15841 aagtaaaggt tctgaaccaa cagctgatac gcttgactat gaaatgatac catactattt
    15901 ttaatagaaa gaaattataa tttaggacaa gaactatgaa cgaagagtta aaagaaatta
    15961 aaattcgcgc ccgaatgatg cctggagccg taacttatga tggttttctg ggaaatgata
    16021 agcgttctct ttctgaaatt attgctgctg ataataaaga actggaacgt tttaaccgtt
    16081 atgttgaaga aatagcagac agaatgaaat attttactga tgccaccttt gatgtgtttg
    16141 ataataaggt tgttatagat gatatatatg aagttgaaag cacaagcagt aaaggtaatt
    16201 tgtcctgccc atatggacac ggaggcttat tccgtaaata tgaaactact ctcactaatc
    16261 ggaagaataa tatcactgtt aaatggactg caatgaccat ccatttaatt gaagctcatc
    16321 atttctttgg ggggaagggt tcacattatc gctatgaacc cgaaactctt ttaaaggccc
    16381 tatttaccga agcagaaagg aaaaaataac tttataaact attctgagta tttcaacctc
    16441 agaattatga gcttatgcta agagtcatta cgactattga tatcatgagt cttcactgcc
    16501 gaatagttag aagcgtttac aagctatttc ggcagtgata actcctgact tattgtgagg
    16561 agttatttgc gcttaaataa cttttgtatc tattaataat ttataaggca tataagagtt
    16621 atcgcgacct cctgccccaa aaaacctcta aaccttcaac aacgcttgga tttggttatt
    16681 cacatccaaa aatgaaatat cacctttggt acggaggtta tctcctttag agaatctttc
    16741 atatagttta tttataattt cgatgatttc ttctgtattt ttactaacaa atccgctttc
    16801 cgttttgcag attaattcgt atgcttctcc atctgaatct gccaaacata aaatagggct
    16861 acgagaagct atatactcga agagttttgt tgttagcatc ccttcattgc cttcgtatgt
    16921 atttataatg agaatcaata gctcggcatt tattaattct tgcaaggcca gttgatgagg
    16981 caaaaaaggt attctattaa ccgggaaatc agtttccgga aaatttcttg ttccaatgag
    17041 tgaaaaatct atgttgggca attttatttc tgttaatgca tttattaaag gagtgggatc
    17101 ctgacctgca gtaagctgac ctacatattt aatacggaat ctattcgttc tttcataatg
    17161 caaatcctta aaatcatcgg gatcaaagcc attgtaaaga acttctttgt taccttcagg
    17221 aagagaattc gcgatgcctt tacttacaat ataattcagc tctgcagtcc gaattatttt
    17281 cctttccaga tatttatgca gagccatagt taaacgcata gggggattca gtttcaaata
    17341 atatatttca gacataggat cgcgaaaatc agttcgccac tgaatattgt agcgtttttt
    17401 tagttttaat ccaatcagat gagtggaatg gggaggaccg gtagttatca cataatcaat
    17461 tttattcgtt cttaaaactt gaactgcact tttcaatgct gccggattcc agccaatacg
    17521 aagatcggga actatgacat ttaagcgtaa ccaatataaa aatttttgta gtggcttatc
    17581 ggttgtttta ttctgtagtg atccataggg tagttctttt tgtcccaata tgttccataa
    17641 ttttccgaaa cttattggtt tacttcgaag tacttttacg gtagcaggaa ttttagaaag
    17701 caagctttca tcccgatatg gataatcacc atctaaagta gtgactacaa atatttccac
    17761 tcctttttta tccagagcat tgataaatct taaccatctt tgaactgcag ctcctccaca
    17821 gggagggaaa taataagtta tcagtaaaat cttcattatt gcaatgttag ttctgctagt
    17881 gctttccagg aataacgttt tgtgaagtta tcaattgctt tccgcatatc cggtttctga
    17941 gtaagatgca aaattatttt ttcagctaga gcagaaggat tatcaggagg aaccaaatac
    18001 cctgtttcac cttcggctac atattctccc aaaccaccta cattactggc taaaacaggg
    18061 actccaaaac tataggaggt ggcaataacc ccactttgag aggcactttt gtaaggcagg
    18121 atacataaac aagattgaga aaagaagtgc gcaatttcag gatcgctgat aaaacgaaaa
    18181 tagcattcta ctttgctccc aattcctaaa gagtttatta gttctgtata gacatcacat
    18241 ttaccatata cttctccagc gataattaac ttggcatcag gtacagcatt tatcactttt
    18301 ggcattgctc taagcaaaac atccagaccc ttatagggtt taattaaacc gaaaaaaagc
    18361 agagtatttc tttgaggaaa aggtacaact tcactgatat aatgttcata acagggatgg
    18421 aaacccagaa ctcctttttt ggaaatatat agaggcagtt cgttatgtaa ttcctccaaa
    18481 gtagctttac ttaaggttac aattttagtt gctggcttaa agacatattt aagcagctgt
    18541 ttttctaaaa tccatttttc gtgagccaga atgttatgcg ccagaataat gataggggta
    18601 ttaggtatgc gttttataat ataaccataa gcaggtgcaa aatatggtaa aaaccaggaa
    18661 acaattatcg catccggttt atattgtttt atttcgtaaa ttgtttgtcc ccaggttttg
    18721 ggttgccaag gaaccagaat tcgcttattg acaaattcat tatctgttat tgtataatca
    18781 tattgtccct tacctggaaa aagcaattgt ggatattgct ctttaaaatt gatgaattga
    18841 acttgatatc ctaaatccct gtaagcttct gctaaagaga aagcaaaaag agaaattcct
    18901 cctctatatg ggggagcagg acccagaata gcaatcctct tcataataca ctctgtttaa
    18961 aaaacctctt aacaatatca acatcgtgat atttcccaaa taaagccata agttttaagc
    19021 ggacttttac acctttcata tcactggcaa gaatacagcc aagagtttct aaatgtttcc
    19081 ctccaccctc ataaccatat tcagctaaaa ctcttcctgt ataggttctg gaggttatta
    19141 caaccaaaat actttttgca atagctgctt gaatatcggg tactagactt ttaggtaaat
    19201 ttccacgtcc aaatgcctct attacaattg ctttgatatc attttccata cagcaacgaa
    19261 tgaattttcc atccatacca gcagcagctt taataagttc cactttggta tctaattgat
    19321 cagtccaaac attatctttg taaagtgaag ttctatgata aacaatttta tcagggtcta
    19381 caattccaac aggtccataa ccaggagaaa taaaggaatc aatttttcca gtatcggatt
    19441 ttaccacatc acgggcagtg tgaatttcat cattcatcac taccagaact cctttatcta
    19501 ttgaatcggg atgacaagca acacgaacac ttccaattat gttgcgaggt ccatctaatc
    19561 ctatttcatt tccagaacgc attgcagctg taaatataac aggtttgcgt gtagtcaaaa
    19621 ctaaatcaca taaataagca gtttcttcta aagtatctgt tccatgagta atcactattc
    19681 catcataatc aattattttc aggtctacca atttagccag ttcaaacatc atttccggag
    19741 tcatataggg actaggaata tttaaataat ccatcacttc aacattagct acactgttta
    19801 attgaggaaa ctgtcttaaa aaatcggcaa attcattact gggaataaca ccagcaggat
    19861 cagacgcctt catagaaatt gttccaccgg ttaggataat aaggatgttc tttttcatat
    19921 ttaatctttt ttccatttaa ttacgataag ggataggatc aataatccct gcttccctaa
    19981 atgctctttt gcgaagcaga caactttcac acacaccaca ggctatctca tcatcactat
    20041 aacagctcca ggataaatga aaaggagctt ttagctttat gcccatttgc actatttctg
    20101 atttgctttt atgtaaaacc ggagttataa tattaattgg taattcactt ttggttcctg
    20161 tggcaattac tttttcgaag gatttgaaaa aaacttcacg acaatcagga taaccggaac
    20221 tgtcttcttc cacagcgcca atataaattt ttgtagcttt tatcacttca gcccaagcaa
    20281 ctgctgcaca tagaaaaata gcattacgaa aagggacata agtaattgga atttctttag
    20341 atgaaatttt gccttccgga acaggaatat gcctatcggt tagagcagaa ccgccaattt
    20401 cagatagcca ttgataatct actaccttag cctgaaacgg tttataataa tctgccaatg
    20461 ctgcaaaaca agcaagctct ttttgctcag ttagctgtcc gtaattgaag tgcaaaaaaa
    20521 agacctcatc aacttcagaa acagcacaag cagcagtaac caaagaatcc atccctccac
    20581 taaccaatac tattgaccgg gtcatatttt atatcctgtg ttatttattt gatcaatttt
    20641 cctcttatgc tgctttgtgt caagataaga atgcactata ctgcaatttt gaaaatatga
    20701 attttagatc ttagggttat aaaaagcaga ataccttaat agattctcta atgatctttt
    20761 actatttcca taacgatagg ttatttcgct gaagaatcgt ttaagtttct aaggttctgt
    20821 aactgtattc tgataggatg gattatgtgg tttccttatc tttaacggca atgatttata
    20881 gttagctgaa taactgaaac aataggcgcc ataataaatc cctattgcag taaatactgt
    20941 gttaaaggga tgaattattc ataaacagat aaattcaaat aaactgaggg atagtatcta
    21001 ttctcataaa ggaaaacctt tcaaaattat ctctttaagt tctgcgatgc tatcagtgtg
    21061 attaatttta ttacgcaatt caggtccacc taaaataccc ttagtataaa aacaaagctg
    21121 agctcgtaat tctcgtactg ctatatattc ctgtttatat tgcaaggctt tatctatatg
    21181 caatataata gtattaagta tatcctgtct ggttacaggt ttatatgttc cacccttcag
    21241 tagttctctg cattgagaaa agagccaagg ttttcctaaa gcacctcggc ctatcatcaa
    21301 gccgtcacaa tttgtttccc taagcatagt taaagcgtct tctggagttt taatatctcc
    21361 attaccaata actggaatag agacattttg cttcagaatt ttgatatgtt cccaattact
    21421 aattccggaa aacatttgac gcgaagttct gggatgcaga caaagaaaat cggctccact
    21481 atcttgcatc aagataccaa aatcaagaaa gttcaaattt tgcatatccc atccgctgcg
    21541 aaatttaaca cttaaaggta ggcaatcttg cagggcattt ttaacttctt taacaatttt
    21601 tacggccaaa gaaggagttt gcattaacgc tgatccagct cctcttcgaa ttactttttt
    21661 aaccggacag cccatattaa gatcaatgaa atcgggtttt aaaggaagta gggtagaagc
    21721 agctttagcc ataataagag ggtcgttgcc aaaaatctga ataccaaaag gtctctcgaa
    21781 ttcttcaaaa tatgcatatt gcagggtttt atccgaatct ctaataatac catcggcact
    21841 aaccatttcg ctgattagaa cttctgctcc ccactgttta caaatttggc gaaaagggct
    21901 atctgtatat ccagctaagg gtgctaaaaa aagtagtggt tctttgaata gatatgtggt
    21961 cattaacccg aatatttagc ctttatagcg ttaatatcta cctcatcaat atattctctt
    22021 ttcataaagc gttcggcgta ttctttataa agaccgctat ctaaaaagaa actaaccaga
    22081 tctttatcca gttgaccatc cttaaccata aatcccataa tagttaaaga ttcggttaat
    22141 gttttagctt ttttataggg tctatcggct gcagttagag cttcgaaaac atctgccact
    22201 gccaaaatac gagattgtaa gggtaaatta tcagctgaat aacccttagg ataacccttt
    22261 ccattaagca tttcgtgatg ggtggaagca taaaaagcta cattctcgta tttcttgggg
    22321 aatgatagtt gggaaagcat ttcccaggtg attgatacat gatcttgcat aattttcatt
    22381 tcttctggag taaaagttcc tctgccacta atcatcatat tctttttttc gtctgcagtg
    22441 aaaatgaaat agtgtttgcc ttcatattcg aaatcaatgt tactgaattt atctactcgt
    22501 tctaaatcgg ttttatctac aaattcttta ccctcattta cattgatcag aaaattagta
    22561 agatcctcaa gatagctgtg aaagtccttc ggttccagct ctaaatcaat ataatgatgg
    22621 ataaaatcag caaaatctga ttcactcagc tttacttgta aataagataa tagcagttcc
    22681 agtttttcaa ttcgtaattt aacaagctca atgcggtcgc aaatagtttc gagttttgtg
    22741 cttttatcca taattgcttc aggagtaaca attttcccta catcgtgcat ccagccagcc
    22801 atagaaagtt cttttagttc attttcgcta aatttgcgtt ctggaaaata tttttcacct
    22861 atgtcattga ttttattggc gatcatttca gttaataaag ctacgcgggt tatgtgattg
    22921 gaagaatatt tggactttct ttcaatagct ccagcaatgg aacggatgaa ttgattcaag
    22981 agttcttcta aatcctcaat caatttttta ttggaaagag caattgcagc ctgtgaggca
    23041 agtgaggata gcattgcttt atgttcttcg gtaaaggaga ttatattgct gttttcatcc
    23101 attgcattta tgaattgaat cacgcctaaa actctatctt cgtggttttt taagggaata
    23161 gttaacatcg acttgcagcg ataattattt tcctgatcgg ttttaatcgt tccgctaaca
    23221 tcgtaacctt ttgcttcata aacatcgtcg aaacataagc tgcgtttgtt atgaaagaca
    23281 ttagttacaa ttttatcatt aactggtgtg ccatcttcat tgtaaagatc aatggctttc
    23341 cagttaatgg gtccctttgt accaccttta agtaaattca aagttgcgtt ataaactatt
    23401 tcaaattcta gtttcgtggc ttcatcgttt actttataga ttgttgctgc atcagctttt
    23461 gtaaaagaaa taccttcctc cagaatcata tcaaaaattt tatctaagtc actttcagaa
    23521 gaaagtgatt cgccaatacg ggtcagacgc ttgatatgca acaattgttc ttctgcgaga
    23581 ttagtgatat tatcaaccac gctgtttagc attttgttaa tctcatagtt tgtgctaaaa
    23641 tcgtatttca ttttgtttac tcctgaatac gctgaaaaag catatattgc ggttcaaaac
    23701 cttgacgctt ttgttcgatt tcgtaaaaag tgctaatatg ctcttcaaaa ggtgaatgtt
    23761 ttaaaactgg ttcatcataa actgagataa aggaaggatg cgttaaaaat tcatctaaaa
    23821 tccaggcagc ataatctcga ttatcagttg taatctgaac ccgtgatgag ggctttagaa
    23881 ttatagataa agcacttaaa aaatcttgct gtataaggcg tctgcgatga tgttttcgtt
    23941 tcggccaggg atctggatat tgaataaaaa cacctgaaac agattcggca ggaaaaagct
    24001 ggcttatatt agcatcaata tgaagtcgga taagacgaac attagggttc tgctcagggc
    24061 ttagtttttt taaagtatta tttatcctct tttctgctga ttccaatccg ataaaattga
    24121 attctggatg tagcacggaa aaaagggata taaattctcc cttaccggaa ccaatttcca
    24181 gtaaaagagg gttggaatta tgaaatattt cttgtggatc taaacgcaaa tcatgaccct
    24241 cagtattaat tttgggttca attacgaaga aatctttgtg attaatcatc gtcttctgca
    24301 tcctctctaa ttgctttttt cgcttcatca atttctttgc gaacgataac taaatttgct
    24361 aaagaaatgc ctacaccaat gaaaataaat aataccaagg catttaaaaa ataatccata
    24421 aaggtgttag cggctttagg ttgttttaaa agtaacatta atatcttcaa atatgttcct
    24481 aggaccaaag ctgaaacaaa tgaaaagatc aaagtccgta tcttgatgag agactttgcc
    24541 aaaaaagtat agcgcaatat caaccaaagt aaaataccac aaatcagatt aagaatgaaa
    24601 agtaagataa ggctaaattg cgagcccaat cgaactatca taagaatccc gtctaataac
    24661 ggtataacaa taagcaatcc ccaaagcgca tatttaggta aacaataaca actaaaagca
    24721 atagcaccaa taaaaaaact aacagctaaa tatagctccg cttgactgat gcctctgcct
    24781 ccccaattat tcgtaaaatc cctaaaacta ccggaaaaaa gcaaaccccc taaaattgct
    24841 gttgctattg ggaaaaacaa caacaacaaa tgccggggca ttattttagt gctttccatt
    24901 caaatccttc acttttatta agttatataa atccatattt caactgtatg taaatttatg
    24961 ttgacttata gacctataat acaaatttaa cgcaactgaa gagaatgtca ataataataa
    25021 tttgatatgg agataaagaa gtgctaaata tagtgaaaag cctgaaagag aaactatcta
    25081 aaaccaatag caacctgatg ggtaaaattg ctgaaacagt taaaaaaaag aaagttatag
    25141 atgaggaaac actcgctgaa atagaagaaa tcctgctata ttcagatact ggaatagaaa
    25201 tgaccgaaat tatcctgcaa cgctttagag aacaattgaa aaaggataaa gttaccgatc
    25261 ctgaaatagc tcagatatat cttactgatg ttatgcggga tattttgcta agcgaagatg
    25321 aagagattcc cgattttttt gcagaaccgt ctgtgaaacc ttatgtgatt gtttttgttg
    25381 gtgttaacgg aactggaaaa actactacta tcggaaaagt tgcctataaa ttttcgcaaa
    25441 tgggcaaaaa ggttttaata gttgctggag atacttttcg ggctgctgcc attgaacaaa
    25501 tcgctatttg ggctgaacga gcaggtgttg ctatagtccg ttctcaacct gatagtgatc
    25561 ctgcagcaat tatatatgat ggggtaaatg cagctatggc tcgtggctat gatatagttt
    25621 taattgacac tgcaggacga caacatacta aagacaattt aatgaaagag cttagtaaaa
    25681 tagatcgcac cattaaaaaa ttgattccag aagctcctca tgaagtgatt ttagtggtag
    25741 atgccacaac tggacaaaat gctatctctc aagcccataa ttttgataag tctatacatt
    25801 taacgggtat tgctttaaca aaatatgatg gcacatccaa aggcggaata atttttaact
    25861 tgaaacataa cttgaaactt ccagtaaaac tattaggcgt tggtgaagga attgaagatt
    25921 taatgccctt caaagctgta ccttttgtaa aagctttctt tgaagaatga taccaggcag
    25981 atatttccta tggatataat atgttgtttc tatttatata aggcagaaat acagataaac
    26041 tgaggattaa ctacaggtct tttttatttg ggaatagaat aacaaggtat tatatctcca
    26101 taattaataa cattcattaa atcttcaata cttcctcaat attgttttgg ggattaaagg
    26161 aaaaaagtac ctcctttttt agtaagttta gttacttaag gaagaagtaa tatatctttt
    26221 ctacagttta ttaggaattc atcctcagta aaaaaacccg aataagaaat ccacatagtt
    26281 tatggacatt ttcagcaact gaatcgctga attaaaatct tctaaaaaca ggtaagcata
    26341 aagcccgtcg gtgagcttcg gtttatttgc caagcaagaa ctagtaatct cattttcctt
    26401 tatatagtaa aattgtgggt tgatattgag tactatccta atgtatttac ctacccgcag
    26461 gtgtttacta atttcggata agctacatat tgtattcaag ctttgctctt cgctcttaag
    26521 ggaaagcgtt aatcaagtgt ttatcaaccc ttaatcaagc gttaattatt aacgcttgat
    26581 taaggcctgt taaacgagag attaaaacct tcaagcaagg aaaacaagcg acaataatat
    26641 tgggatttta ttatcaaatt gattgttaca gttggtataa ttcagtttca agagggaaat
    26701 aatcgcaata ttttgaagtt tcaactttaa tcctcccaat tgtaattgat ccttatatga
    26761 atcttaagag gaattaaaac tgaaggagag gatactttct acctttacca cccaacctca
    26821 atactgtttt taattgacaa aagaacctat actaaattaa ggataactta atttaataac
    26881 ataaaaaagg agatctaaaa tgtctaatca acctccccaa aaccaaattc agataaaaat
    26941 cgacgaaaag gtcggtgaag gcacctatgc caatttcttt gttattacca attccccttc
    27001 agaatttatt atggattgtg gtcgtatcct tcctggtatt cctgatgcta gaatttatac
    27061 acgcgttctg atgactcctt ctcatgccaa acaactgatg caacttttgg aaaaaaatat
    27121 agatgcatac gaaaatcagt ttggcgaaat caaattacaa ggtcaacaag ataataaacc
    27181 tgttggtttc aagcccgatt ctaaaatata aagggtgaaa aggtcagtag ctaagagggt
    27241 gaagagaaaa ggttaacaga ttaactttag aactcatatt atggaaaggt gataaggtaa
    27301 cacctataaa aaaatgcata gttgcctttc acttgttaat ctgttaacct ggataatctt
    27361 gctaaaataa cctatgcaag ataagcgcta taggtttgct attatctatc ttccttgttt
    27421 gatgaccaag atttagcttg acgaaatatc cttatttatg agataaattc tttaatatat
    27481 gatattgcta tcacagtaat ctaaatactt aactatcaat atataataaa aggagatata
    27541 aggaaatgag caaaattgct aatatgctgg atgccgattt ggagaataaa atttgcctgg
    27601 tgcgtatgga tcataatgta gttaaaaatg gcattattaa agatactatg cgtattgatg
    27661 ccacaatacc aacccttatg cacatattca gaaagggagg tttaccaatt ttgatgagcc
    27721 atattggtag accctttgac aaaaaaaccg gtactatcaa catttcggaa gctgaatcag
    27781 ttgttccagt tgtaaaatac ttggaaagta aatttcagct tagaggttta atacctgatt
    27841 gcaagcccca agataaaatt gggataacag attatactcc gattggaaaa gctgttgaat
    27901 ccttaaaaaa gggtgaagtg gattttgtat accttcctaa tactcgctgg tttaaaggcg
    27961 aagaagcaaa agatgaaagt actgatgtta tggcaaaaag tttagcacaa tttgctgatg
    28021 tttatattaa tgatgctttc ggttcttggc aagcacattg cagtacttat acaattgcca
    28081 attaccttcc ttcttatgct ggacttttga tgcagaaaga aatatccaat ttacaaaaga
    28141 tctttgaacc taaacatcct tttttagcaa tagtagctgg agcaaaattc gatacaaaaa
    28201 tcgggccttt atcttccttg atcaaaatag ctgataaact tattttaggt ggagttattt
    28261 ataatgcata tctctgctgt aaatataaca ttaaaattga aggtgtaact aatgaagata
    28321 ttgaagcagc caacaaattt attagtgaaa gtagagatta tatacataag atagtggaac
    28381 taccgctggt aattgaaaat acaggaatgc aaaagcgcga aaatccgaaa gagatagata
    28441 tccatatttt agaaccagga acacaattag catatgtctt agatgtagca ggtagtaatt
    28501 ttgctgagga atgcgtaaaa aaagtttttc aggaagcagg aactatcttt gtaaatgcag
    28561 tgatgggtta tagtgctctt tttcctgagg gaacaatggc tatgtactct ttgattgacc
    28621 aatgcaaaaa tgctgtaaaa cttttcggtg ggggagatac aattcaggat tttaaagaat
    28681 accttcctgg aatttttgca aaagcacaaa acgatcccag ctactatttc tttactggag
    28741 ggggcgcgat tctggatgct attcaacagg gctctccata cggtatgaaa ccggttcaag
    28801 cattacttag agattaataa ttataggaga ataaagtgga taatgacaat aaactgaacc
    28861 tgctgaaaga atttcctcct ccaacatatg aggaatggtt gcaggtagta aatgaaggtt
    28921 tgaaaggtgc ggattttgaa aaagtaatga aaacccaaac cccggaagga ataactttgc
    28981 agccaattta tcgtaaagaa gatgttgcta aacttccttt tatggaaagt atgcctggta
    29041 aaagtcctta tgtgcgaggg aataatccta tccgtttttt aactgagggt tggttgattg
    29101 ctcaaaatca tacagattct gatctaaaca ctttaaacca gaaaatactg aaagaattga
    29161 atcagggttt aaccgcagtt aatattacct tagcaaataa tacctctcct gaaggggtga
    29221 aagtacaaac tcttaacgat tgggaaacct tgttggcaaa aattgacata aaagctgctc
    29281 ctttgtttat gcaattaggt ttagaggata cagaaatcct gaaaacccta aaacaatata
    29341 ccgataaata caatattgaa ctaaagaccc ttcaatctgg cataggtatt gattttatcg
    29401 gtgaattggc agatagagca tatctgcctt tttctcttga agagacctgg caaaaattag
    29461 aagaaacagt cagatggaat atagaaaatg catccgcgaa caggataatc agtattgatg
    29521 cctctattta tgaatcagct ggagcttcca ttgttcaaga gttgtctttt gctttgtcta
    29581 cagcaatttg ttatataaaa cacttacagg atgcaggttt taccattgaa gaactggctc
    29641 ctctatttca agtgaaactt tcgcttggct ccaatttctt tatggaaata gctaaaatac
    29701 gtactttccg tttactttgg tctgaaatga ttaaagcata cggtggggaa gaagaatatc
    29761 aaaaagtatg gattcatggc aaaacagcaa agttcaataa atctatatat gatatatatg
    29821 taaatgtgtt acgaactaca actgaaggtt ttgcaggagt gattggaggt gtagatagtt
    29881 tagaaattgg ccgcttcaat gaattaattg ctcctgcaga tgaattatca gttcgccttg
    29941 ctagaaatca acagattatt ttaaaagaag aagctcattt tgataaggtt attgatcctg
    30001 ctggtggctg ttattatatt gagtggctta ctaatgaatt ggcaaataaa tcctgggctt
    30061 tgatgcaaga aattgaagca aagggtggaa tgctggaagc gttgataaat tcagatatac
    30121 ataattatat tgaatctgta gccaagcaga gaattgaggc agtaaataga cgcaaaagtg
    30181 tttttattgg cgttaatatg tatgccaatc cactagaaaa tatggtttct gcattgcctc
    30241 attcaaattc tacttgtgat gaatttacaa caacaattaa accattacca aagcgaagag
    30301 ctgtagaaga tgtggaaaat cttagaatgc agattgaagc aagcaacgct aataaaaaga
    30361 tctttttact taatatggga acccttgccg aatataaagc cagagctgat tttgctctta
    30421 atttctttca agtagcttct tttgaagtta tctatcccaa tggctttgcc aacatagatg
    30481 aagctataaa agaagcggaa aaatccggtg ccaaagcttt ttgtatctgt tctacagatg
    30541 ataattatct caatttggtt cccgagattt gttcaaaact aaaaggtaaa attatcagct
    30601 tagcaggcta tcccacagat aaaattgagg actataagaa agcaggaata aatagtttta
    30661 ttcacatcaa agcagatatc gtaactaccc tacgggaatt ggcaaagcag atggaggtga
    30721 ttaaatgaaa aatcctgatt tcttaaagat taagcccgat tttattgctg agcagaaaac
    30781 ggaaccgaaa cttgatttta gctggaaaac taatgaacag atagaagtta aacccctatt
    30841 ttttaaagcc gatatggaaa atatcgaaca cttagattat ctatccggtt tacctccttt
    30901 tctaagagga ccttatccta caatgtatat tcaacgacct tggacgattc gtcaatatgc
    30961 tggtttttct acagcagaag aaagtaatgc cttctaccgt aggaatttgg ctatgggaca
    31021 aaaagggcta tcaatagcat tcgatttacc aactcataga ggttatgatt cagaccatcc
    31081 tcgcgtgatt ggagatgtag gaaaagctgg agtagctatt gattctattt tagatataaa
    31141 gatcctcttt gatcaaatcc cactggataa aatgagtgta tcaatgacaa tgaacggagc
    31201 tgttattccg ataatggcat tttatattgt tgccgcagaa gaacaaggtg tgaaaccgga
    31261 agctctaaat ggaacgatcc aaaacgatat tttgaaagaa tatatggtgc gtaatacata
    31321 catctatcca ccggaaccct caatgcggat aatagccgat atattcagct atacttcaaa
    31381 aaagatgcct aagttcaata gtataagtat ttctggatat catatgcatg aagcaggagc
    31441 tactgctgac ctcgaaatgg cctatacctt agcagatggt ttagaatatg tgcgtactgc
    31501 tctaaaagct ggtatagata tagatgttat agctccgcgg ctttctttct tttgggcaga
    31561 aggaatgaat tattttatgg aagtggcaaa attgcgtgga gcaagaattc tttgggcaaa
    31621 aatcattaag cagtttaacc ccaaaaatcc taaatctatg gctttaagaa cacatagtca
    31681 aacttccgga tggagtttaa ctgaacaaga tccttacaat aatataacaa gaacttgtat
    31741 tgaagcactt gcggctacaa tgggtcatac tcaatccttg cataccaatt ctttggatga
    31801 agcaattgct ttacctactg atttttctgc aagaattgcc agaaacacac agctctattt
    31861 acaaaatgaa acaagcatct gcagagtgat tgatccctgg gctggttcat attatgtaga
    31921 atatttaacc gatgctttaa tgaaaagtgc ttggaatcat ataatggaag ttgaaaactt
    31981 gggtggaatg gccaaagcta ttgaaacagg gcttcctaaa atgcgaatcg aagaagcagc
    32041 tgcccgacga caggcaaaaa ttgattctgg tgctgatatt attgtgggtg taaataaata
    32101 ccctccggaa acggaaattc caatcgaaat attagaagtg gataattccg ctgtgcgagc
    32161 cacccaaatt gcccgtctgg aaaaactacg caaagaacgt aataacgaag atgttgaaaa
    32221 agccctggaa gcaattacca aatgcgctga aacaaaaaat ggaaatcttc tagaactggc
    32281 tatcgatgca gccagaaaaa gagcttcttt aggtgaaatt tcctatgcaa tggaaaaagt
    32341 atgggggcgt caccaggaag ccattcgttt aataaataat gtttatagca gtgagtatgc
    32401 aaaaatggat gatattgaac aagttcgtgc tttaactgat aaatttgcag aactggaagg
    32461 tcgtagacct cgtatactag tagccaaaat gggccaagat ggtcatgatc gcggagccaa
    32521 agttgtagct actgcctatg ctgatatggg ttttgatgtt gatgtaggac ctctatttca
    32581 aactcctgaa gaaacagctc gtcaggcagt ggaaaatgat gttcatatta ttggcataag
    32641 ttctttggct ggtggccata agaccctact tcctaaactg atggaagagc ttaagaaata
    32701 tggcagagaa gatataattg ttacagttgg aggtgtaatt cctcctcagg attatgagtt
    32761 cctttatcaa aatggagctg ctgctatatt cggacctgga accaaacttc ctgaatctgc
    32821 aaaattagtt atggaaaagc tattggcaca caagaatgaa tgaaaaaaga aaaccggaat
    32881 ggactccttt atatagtgga aaggaatttg ccacgagtgt tgtaacagga aagaaatctg
    32941 tgcatataaa agcgtctcct tctactaaag ggaaaaaata cgatttagat actttggtaa
    33001 agggagttct aaattccgag cgaaccttgc tggccaaagc aattactttg atagaaagta
    33061 atgcagaaaa acacttttta caagggcagg agctaattca aaagctcctg ccttattctg
    33121 gaaattctat tcgtattggt ataacaggag ttccaggagc agggaaaagc acttttatag
    33181 agagttttgg cttatattta attgaacaag ggcacaaagt agctgtttta gctatcgatc
    33241 ctagctctac tattagtaaa ggaagtatat tgggcgataa aacaaggatg gaaaccctgt
    33301 ccagaaatcc caatagcttt attcgtcctt ctcccagtgc gggaattttg ggtggagtag
    33361 ccagaaaaac cagagaaaca ataatattat gcgaagcagc aggttatgat attgtattaa
    33421 ttgaaactgt tggagttggt caatccgaaa ctactgttcg ttctatggta gatttctttt
    33481 tgctgatgca aattgccggt gctggagatg agcttcaggg tattaaaaga ggaattttag
    33541 aacttgctga tttgattgtg tttaataaag ccgatggaga taatatccaa cgaacggaaa
    33601 tcgctaaaca agagctacaa aatgttttac attatatgcg aaatgcaacc gagggttggc
    33661 aaactaatgt tgtaacctgt tcggcaataa acaaaaccgg tttagacgag atttggcatt
    33721 cgatttcgga attttacaca aagacaaaac aaaatgggat tttcgctaaa cgccgtgcac
    33781 agcaaaatac acagtggttt gaaaatttga tcacagaagc agttttgcat cgtttctacc
    33841 agaaagagga agtgaaaaca ctcatcactc agttgaaaga cgatattgct atcggtaaga
    33901 ttcctattgc tttagctgtt agcaaaatac tgaaagcaag cgaataatat cattcggggt
    33961 acttataatc aagtaccaga atagttatat catccctttg cggtatactt tgtgcaaaat
    34021 tatgcacata ttgcataatc tgcttagcag tttgttccgg attggtagtt tccaggttct
    34081 ggattattga ttcaacacct tttatcccca gaaaatcttc tttttcattc ataatttccg
    34141 taaccccatc cgttaggaga ataagttcgt ctcctttatc cagtttcaat tgggaggaac
    34201 caatttccat attttcaaaa acacctaaag gattggaatg agtatcagcg aaaatttcat
    34261 aagtaccatt cagctttctt aaaaacaagg gagtatgacc agcattggaa aattccagtt
    34321 catcattttt taaatctatt attcctatta gagcagtaac gaaaatcgcc gcggaagatt
    34381 gagtacacag aaaattattg aggtttttca ttatttcatt tacagtgaag ttataagtgg
    34441 aaatagttct tagataagta gaaaccatta tcatcgtcat tgaagccaca atgcctttac
    34501 cagctacatc agctataaca aaaaagaaat gagattcatc aacaggaaag aaatcgtata
    34561 aatcacctcc gatttcacca gctggttcca aaataccata acagcggatt tcctttactt
    34621 tgtagggatg ttcagtattt gcaggaatta gttttcgttg aatttcagaa gcaacttgta
    34681 cttcagccag aatgcgattt ttttcagcat ttgtaatttc caaatttttg atatactctt
    34741 tcaaagattt ttgcattaca gcaaaagaat gtataaggtg atctatttca tagctgtttc
    34801 ctgaaggagg aagctgagca tcaaaatcac ctgaacctat tttagtggct acttctgtaa
    34861 aaagtctaat gggtttagag acactgaaag ttctgtaata tacaattagc gatatagata
    34921 taaatataat caccgaaaag agggtttgaa taatcagcaa caaatttagg tctctaacga
    34981 catctttatg cgcaataaca attcccagtg accagttatt ggaaagcagg ggagaaaaat
    35041 atatccaaga atcgttaaaa attgtatttc ctcgtaaacg aataaaatca gtttcacctt
    35101 gaatcatttt tttgccgagt tctcgtaatt gggtgtcctt tgtttcttcc gctaagctaa
    35161 atatgctttc attaaaagat atagaatctg caggatgagt gataatggta ccaatgctgg
    35221 aaactaaaaa agcataacca ctgcgtttta gctttagagg agaaacgatt ctctgcaaac
    35281 cggaaagttt tgtatcgaat cgcataacac caatacaatt acctttaata aaaataggaa
    35341 cacaatacga aataactgtt tgctttgtgg aactagcatc ataccagggg tcagtccaac
    35401 atttactttt tagttcatag ggaatttgaa accaatcaag atattgatat tctgcaggat
    35461 gcaattcctg agatatggcc ttatttttaa agtgataaat ggttcgggaa tcaccagatt
    35521 tgttatactc tggagcataa gcaatagtaa ttgattccaa agtgtcaaat tcatagatat
    35581 aatcattcag aattgtatcc agttcgtttc tattgagata gtcagaatta tataatgtta
    35641 taatcgattg agagatatct actatggctg ataagcgtcc ctcaatctga taaacagttt
    35701 ccttagcaag atgacttaca gtttcacggg aattatccat cattatttcg gaaaacagaa
    35761 atctggttat tcctataaag acagagaaaa taagtaccag aaaaaaaaag ataaaaacaa
    35821 ttatctggta agcaagtcct cgttcttctt gtttactttg catacggata aaaactatta
    35881 taggtttggc tggttatagg catattttgt tcagttagta atctctttgc ttcttcaaac
    35941 tgactttttt gaagaacacc aatttgacta ctatccacca taaaatcttg gcttaattca
    36001 tttagcatcc atttttgatg aggtctatta gctggtaaat ggtgtttatg cataatttct
    36061 aaaactgtgg atattgtttc ctcttgattg tttattgcat ataaccaccc ttcaatagtg
    36121 gcgtcaacga aatcacggca atgtttcgaa tgcttttcat aaaattccct agttgtataa
    36181 agcccattat ctgcaacatt aagtccaata tctgcaaatg atactgtaaa taaatcctca
    36241 atatctattc ctgcttgctg aagttgatga tattcattgt aacgcatcac attcattata
    36301 tcgatcccat ctttcagaaa gagattgatt gatgtatcca aatctacaat atccatatta
    36361 agcttgtgct gctgtagaaa gattaatgag aggaggcggt catcagtttt ccacaaacct
    36421 attctctttc cattaaaatc agctatcgag tttattcctc tggtttttta cctaccaaca
    36481 tcagataggt tcgctgcatt aattgcgcaa gattaactaa attatctttt tcagaatctc
    36541 tttttatagc agttaatagt gacatttgca caatatcact atgtccatcc tgtaaattgt
    36601 cgtaaggagg atagtgggga ccaccggttt ggattagtac attcaaccct ttatcgttat
    36661 aaaatccttt tttttgagcc atataaacac cggcaaattc tgcttgatga gtccatacag
    36721 gaagatagcg aatagtgaat aatccagtac tatatggtga ttgttccata ttggattgag
    36781 ttatagcacc gttttgttta tcggattttt tgcatcccgt taataagaag attgataata
    36841 aaagcagtag taaagctagc gtttccctct tattattcat atagatctat tcctctctta
    36901 acatgtaacg caataatgta tatcatccca tgaaactccc tatggccatt atagctataa
    36961 tacaatactg ggcataacaa catcctaccc aaagttacga agaatctctc taagaagtaa
    37021 caatccctgc tatcttctct caataaatat aacagattca tattcgttag tgcaagaaaa
    37081 tgtacatacc taaacaagga aattcgattt tttcttgcac aggcagaaaa tctgctatta
    37141 taaatcccat atctttctta ctacatatta acaataatat aaataaatat cctgtcaaac
    37201 agaatcgaac aaatatcctg tcttttcgtg ttgataaatt tctacagcgc ttacatttct
    37261 tttatatagg actttattgt gaaaatattc gacagatcat ctgtaaaaag cacaactagc
    37321 taattagata taatgtatct catcatcatt agcatgcaaa ctgtaaccct cctgtgttgc
    37381 gaggggaggg ttacagaaaa ttcattatct acatagcagc tttactaaaa taacatctta
    37441 ttatgttggt gctttaaaat aataagtatc tatcgctatg tttttgttat aatggaagaa
    37501 accgcaacaa ataagaacat aatattccag tttattgtac gctgtacttt ttgtttgagt
    37561 agacaatttc cgtttatggc ttcttaagtg acgcggcttt gataaattct ctaaataatg
    37621 gatgtggatt agtgggtctg ctcttaaatt ctggatgaaa ctgcactgct ataaagaaat
    37681 ccaaatccgg atattctact atttccacta aataattatc aggtgataat ccactaatct
    37741 ttaatcctgc cttttgaatt atttcgcgat aatcattatt aaattcgtag cggtgtctat
    37801 gtctttcact aatggtttct gtttgatacg cttgataagc tttactacct ttatccaaat
    37861 ggcaatcata agctcccaag cgcatagatc cgccaattct tttttggtaa acttgatctt
    37921 taataagatg aattactggg gctaaagttt cttcgttaaa ttctgtacta ttagcatcta
    37981 caagattaca aagatctcgg gcacattcaa ttactgcaac atgcattccc agacagatac
    38041 ccagataggg tatattattt tctcgggcat atttggtaaa aacaattttc ccttctatac
    38101 ctctaacacc aaatccacca ggaactaaaa taccttgaac accattgagc atagttgcaa
    38161 tttcttcttc caaaaaattg tgttcgctat caatccataa aatttttact cttttcatca
    38221 aagatgcacc tgcgtgaatt aaagcttctc caatactttt ataggcatcc tggtgttgca
    38281 catattttcc acagacagcg atagtaacaa cttcctcagc attttcgcga ttatgcaaaa
    38341 aatgcaacca ttcattcata acaattggtc tttgttcaag tttgaaatgc tggcaaataa
    38401 tttcagggag ttttgccttt tggaaattaa ggggaatttc ataaacacaa tctacatcaa
    38461 ttgcattaat cacattctct cgtttcacat tggtaaacag ggctatttta ctataaatct
    38521 cttcttcaaa aggtttctcg cttctgcaaa gtaaaacatc cggttgaata cctatttccc
    38581 taagtttata agcactatgt tgagaaggtt tggtttttaa ttctccggct gattttatat
    38641 atggtacata agttaataga atattaagcg tattttcata accaatatct aaacgtaatt
    38701 ggcggactga ttccaaaaat ggcaaacctt caatatcgcc aacagttcca cctacctcgg
    38761 tgataattat gtcatactct ttatccaatc gttgaatacg atttttaatt tcgttagtta
    38821 catgaggaat cacttgaact gttttaccta aataaacccc attgcgctca ttttggataa
    38881 cactttcata aatctgacca gcagaattac tggaataacg agttaaagct tcatccacaa
    38941 accgttcata atgacccaaa tcaagatctg tctcagcccc atcgtctgta acaaaaactt
    39001 ctccatgctg aaaaggactc attgttccag gatcaacatt cagataagga tcaaattttt
    39061 gcagaacaat tttatacccc attgatttta ataataggcc aatagaggcg gtggcgatgc
    39121 cttttcccaa ggaagaaagc acgccaccca taacaaatat atatttcgcc atctggctta
    39181 tctcttataa catctttttc acttctgcaa gagattcttg gattgctccc acagtgtaag
    39241 ctaaatcttc atctgtatga cgatagcaaa tataaccatg atgatatggt tgtaaaaata
    39301 ccttgcgtcg tattaattgt gtgtaaaatt ctactctcag tttcttatac aacccctgat
    39361 catctttttt gaaagttata taaggcatcc aaggtgctcc tgtaaagttt acaggcactc
    39421 cagaattagc aactactttt tccacttcgg ttccaaattt tcttcctttt tcggctacaa
    39481 catctaaaac cttatctctc tctaaaattt caatggtttt cagagctgca acaataccat
    39541 cactattagg aaagaaggtt gaggataaaa agactttatc agctagaacc ttcatatatt
    39601 cttcttttcc cactaatgcg gcaatagcat atccattagc catcgctttg ccaatagtgg
    39661 aaaggtctgg agttattccg aataattttt gagctcctcc caagctgcat ctgaagccac
    39721 tacggatttc atcaaatata agtaaacaat tatattgatt tgccaattcg cgtacagctt
    39781 ccaaataacc aggttttggc atttgtactt ctgctccatt aggatgacca acaggggtta
    39841 caattattcc tgccatatca tctttgttgg cttttaatat tgcttccaga gaatcaatat
    39901 cattataatg aaattcataa atatcttcat agagcttttg aggaattcca cctttcactt
    39961 caacgcacca atcgtgccaa ccatgatatc cacaccgggc aatttttgtt tttcctgtat
    40021 atccacgagc tactcgaata gctattgtag ttgcatcact tcctgtttta actaatgcag
    40081 ccatatcaca acaaggaata agttcacgca gcttttttac taaagcattt tgtactgctt
    40141 gggttaaaga aaagcaaatg cccttgttct ttatctggtc tattactgca ttatcaattt
    40201 cttcttcacg atacccaatt attattgggc cataagcaca taggtaatct atatattcat
    40261 taccatccac atcaaaaatt cttccgcctt tgccattatc aaagaaaata ggatattctc
    40321 ccgggacgaa gttatagggt cttcttattc ctgctacacc gcctggaaca agagtttggg
    40381 cttcttcaaa tagagccata cttttttcaa gttttagctt gtctaccatt ttattctcct
    40441 tatattataa tttttattac ctcgaaatta tcgaaaaaac agagatcacc gagagaaatt
    40501 gataactaca gttctcatac ctcgttttat cagtctataa tatttttatt atagactaca
    40561 ttacctctta ttaactgtta cctgctttat atcgtacctt tacaatatgt ccttgtcgtt
    40621 agaagattcg gtgttctttt ttattagtgt tctcaaaatt ctttttttta tctttcccag
    40681 agtgtagcta tccccattcc gccaccgata cacaaagcag aaagtccttt atgaacattt
    40741 cttcttttca tttcatgcag caaagttaca attattcggg ctccacttgc gccaatcgga
    40801 tggcctaaag ctattgctcc accgttaaca ttagtgattt caggattaag tttcccaatt
    40861 ccttccagtt ccaatccttt gaaaacagca agtgactgag cagcaaaggc ttcattcagt
    40921 tctgcaagct ctatatcact taatttccaa tttgcttttt gtaacacttt tttaattgct
    40981 ggaacaggac cataacccat aatagaagga tcaacacctg ccgaagcact gttaacaaat
    41041 gtggctagag gtgttaaatt cagttctttt gccttatcag cactcatcaa aattaaaaaa
    41101 gcagcgctgt catttattcc acttgcattt gcggctgtaa cggttccatc atttttaaaa
    41161 gcaggacgca gcttagatat ggattcaaca gttacaccag ctctgggcat ttcatcttta
    41221 tcaactataa tagggtctcc ctttctttgg ggaatgataa ccggaacaat ttcatcctta
    41281 aatctatctg ttttctgagc tgcctctgtt ttattctgac tactggcagc aaaagcatcc
    41341 tgttctgctc tggaaatatt atatttttca gctaagtttt ctgctgtgac acccatatga
    41401 caatcgttaa atatatcact aagaccatcc cggatcatta aatctaccat atctttacta
    41461 cccattctgg ctccccaacg agcatctttc aaagcataag gaatctggct catattttca
    41521 gcaccaccag caacaatgat atctgcctct ccagtactaa tcatcaaagt agctaatgaa
    41581 actgctttca ttcctgaacc acatactttg ttaactgtat aagccggaac ggtttctgga
    41641 atgccactgt ggatagctat ctgacgcgaa acattttgtc ctaaacctgc accacaaacg
    41701 tttcctataa taacttcatc aatcatatca ggtttgatac ctgtttcagt aaacattgct
    41761 tttaatgtgt ttactcctaa ttgcactgcg gagatatctt taaagacacc tccaaaatta
    41821 cctatgggac tgcgttttgc acaaactaca acgacctgtt tcatttgttt ctccttatca
    41881 gtattattta tccatttaca ccaacacaaa aagaaagcga gttttcgtca atgagaatct
    41941 tgaaaaaagg gaacaggtta atagagattt aatatatatg cttacctcga gagtgagtta
    42001 aaaaatctaa aaatcatcaa atcataactg aaaaaaatgg agcgggcaac gggactcgaa
    42061 cccgcgaccc tcagcttggg aagctaatgc tctaccaact gagctatgcc cgcgtattag
    42121 atgaattcca ataattggat gagggcttta aagtcaagaa aaacaataat gatgcaatgt
    42181 ggtaaagctt aaaagtatcc ttccttaagt acttatttct ctaagattca tataagtatc
    42241 aattgcgatt ggaaggatta aagtaacaac ttaaagatat tgaggtattt tttccctgga
    42301 aactgtatta taccaatctt aacaatcacc tttataatga aatcccatta ttattgtctt
    42361 ctatattcct tgctgtgagc tattaatctc tcgtttaaca ggccttaatc aagcgttaat
    42421 aattaacgct tgattaaggt tagattaaca cttgattaac gctttcgctt aagagcgaat
    42481 accggatctt gcatacaata tgcagtttat actaaagtag taaacacctt atggtaggtt
    42541 aatatattcg gattgtactt gatatcaatc ccaaatatca ccgtttaggg taaaaagagc
    42601 ttaccaattc ttgcttgccg aataaaccga agtttgctaa cggaccttgt gtttacctgt
    42661 tttgtagatg atatgaattc ggcaattcag ttgctacaat cttaaagagt aaatatcagg
    42721 tagtgtagga ggttgtaatt aaaacctgca tttctcctat aattcttttt actgctgggc
    42781 tgatcagatc catttttttc aattgttcta ggcgattgtg acgctgttct aattcttctt
    42841 gagtaaaaat tttgtccatc aaattgcaga tattaagtaa agctataaga gctaaatctc
    42901 tcggttcggg ttcaatatct gtaagcacag tttttcgaat ccgggaaagg acttcttcct
    42961 cttctttttg atcaataaca ggataacggc gttgtttaaa taaccaaaaa accttacatt
    43021 ggcttacttt aaggatgcct ttatcttcta aatcttgcag tagtttattt tttaaatgac
    43081 ccatagaatt ataaagctga gctactaaat tagcaggttc attaccagtt tccgaattca
    43141 tcaaattttc taaaacagta tccagaaatt tgtctcctgt tttttcggta gataggatat
    43201 taatgccttc catatctaca tcaattcttt ttcgcaatgc caagtccatt aaaagggcac
    43261 caattaaagc cacattgaag ttgatattgg ttatcttgta aaaagtgtta gttttatctt
    43321 ctatagcgag caggattaat tcttcggcac agttaagcat tttaatctcc tttttattta
    43381 aacaggttat attacagatt cttgatgtat tgttcctcat agatgattga tttaaacttc
    43441 tcgttcattt catccaacgc ttctttgggg gtagatattc cacgcattgc tttttccaga
    43501 tatcccttta gttcaagtcg agctttgaac caagcgggaa tttgtggttc aaaaacagct
    43561 gtttccaatt ggttataaat accttgaaac tgaggataat ccttcagaaa atttttcagt
    43621 attgccgatt gcatagcact ctttcgtaca ggcatataac aagtatcagc actccatcgg
    43681 gctgtttgat tggtatcagt aaaccatttg ataaattccc atgcagcttg ttctcttttt
    43741 ttatcaccac ttttaaaaat cacgatattg gtcccgctaa cggcactttt atttgttcta
    43801 taagtaggta gcggtgcata tccaatatta aaattgatcg gttgttgacg catatgtgta
    43861 atggatacac tacttccttc atacatagca gcgattccag ctaagaagtc attttgtcct
    43921 tcaaattcac gaaccaaata tacggtctta tctttattca ggaggtcggt aataaaagtt
    43981 aatgcctcaa ttccttgagg gctatttagt tgaggattac ctagttcatc taatatagct
    44041 cctcctgcct gatagagaag attgatgaac tgccattcat tcacattgaa atttgtgcca
    44101 taggtttcag gtatgccatc attattagta tcagtagtca atttacgaca atataaacga
    44161 aattcattcc aggtagcagg aaaatgattg ggatcaagtc cagctctata aaaagcatcc
    44221 ttattataga aataggcacg aacgctttta ttaaaaggaa aagaatagat agtgtcatta
    44281 aatgtattgg atttcaaaaa aacaggatag atatcttcta agtcttcttt tgtccaggtt
    44341 gtatccgcgc gcattaactc ctcaaaatta caaagtaccc ctgcctctat gtatttggaa
    44401 gtccaagatt caaaaacttg agctatatca ggctgctttt ttgcctgaat ggaagccata
    44461 agtttttgag agagagccgt gtaactacta ataggattgg ttataacttc tacattttta
    44521 tgagtgcgat taaattctcg gatcatttcg ttaagagtat ctcccaaagg accacttaga
    44581 ccatgccaga aagtaatttt tactttctcg gactgcaaag cacttttaca acccgctaac
    44641 aggacaaaga tcgttattgc caaaatgaac agcttgcgca tagttactcc ttttctttta
    44701 taaaaaaacg aacctaccgt cattattatt taaagggagt tggagtcaat gaaaaagtgt
    44761 tagatattac ttttagaggt atacaaagga aagctaacat agtaaaaata tatgtataat
    44821 gaataattgc gtattcacaa tggagaatga aaataacact acactatata aaactcatgt
    44881 tgcttactct tcattgacgc cattaccaat aattatttta ttccagagtc cttttaaatc
    44941 tatgcactgc atagttatat atcaatattc gttcgattta agctatggta gctgggtcct
    45001 atttatccgc taatccacat atctactaat ttgggtaata attctccaga tttaccttga
    45061 tagaattcat ctatataacc aagattgtca ggtttttcaa gattaatagc tacagtacgg
    45121 gcacccataa gtttggcagt aattacaaat cccgcagcgg gatatactac accgctggtt
    45181 ccaattacta ttagcacaga acattctctt agggcatcat caatcatatc caaatagtgg
    45241 ggaaattcac caaaccaaac tatatctggt cttaaaaatc cctggcaata tggacataag
    45301 ggaattgcct tgtttaaatc tatatctttg atagctaatt tactctggca ttttgtacaa
    45361 aggcaattag aaagcgaacc atgcatttct aaaactctct gtgaacctgc cctatgatgc
    45421 aaacgatcta tattttgggt aaccaacaga aaattatcac ctaaaaagtt ctccagtttc
    45481 accaatgcta aatgggcagg attcggtata cgggataaac tatcattata tctttcccga
    45541 taaaaattcc aaaccataac agggtttgaa aaaaatgctt ctggggttgc cacatcctca
    45601 actctatgat cttcccacaa tccatcacta tcacgaaaag tacgaattcc ggattcggca
    45661 cttattccag caccggttaa tacaacaact ttagtattag gtgtaagttt tagcatcttt
    45721 tttatttaat cagtagcatt ttatgggtta cagtaccaga aggagtcctt aaacggtaat
    45781 aatatactcc acttccaata ttatgcttgt gcatatccaa accagtgaaa acaactttat
    45841 gaattcctgc atccattata tcgtctatca aacggtttac caattgtccc ttgctgttat
    45901 aaacctcgag gcatataggc atttttgagg gtaattgaaa ttcaatggtt gtttctggat
    45961 taaagggatt aggatagtta ttgtgcagaa caacattttc aagggaaggt gtagtttcgt
    46021 ctatattatc aacaatacct gtaatattaa catcatcgat ataccaacct tctccaccta
    46081 cataagcatc actgccaaaa atccaacgaa attgagctgt gccgctaata ttgccaagtt
    46141 caaaaatggc ttcagaccaa ttaatagtac ctgaatatac ccaagtatta gcaggaaaag
    46201 gagatgctga attattgtat attttataag gatacccgcc cacaggagta atttgagtcc
    46261 aatcgcctcc atttaaactc atttcaacca aaccaccatc ccaagcataa gattgatttg
    46321 tactgtgctt ttctgcatcc atccaatgat agaactttaa ttgacaattg ggatttacat
    46381 tcataacagg actaataagt gctccgtaag tgctaccagc atattgtcca gaaccattac
    46441 ctccaaactt catagcataa gtaccattag gagtgtgatt tcgagtagat gaacgatgcc
    46501 attgattaat aaaattactg ctgagtgcaa cagtttccca atcttgatag ttcgtttcaa
    46561 aactgtatga aatggcacca agatgaacta aaaagctacc ttcaatcaca tttccgttat
    46621 ctgcgctgaa aatgtaatct atagtgtaat tatcatctat agatgcacta tctaaaatat
    46681 ggaaggatat tacattctga aaatttaaag tagtattagc ataaatggga tccagaacta
    46741 cttcatattg atccagaatt atatcgctat aggagctgaa tagtattaat attggattat
    46801 aggcatttcc acttccagta ttagatatac tgaaattgat tgttggtgaa tctcctggca
    46861 taattattaa actggaatta tgaatttgat atgatgttaa atctattatg ggagcattta
    46921 atgttatata tgcaatagaa gtagaggtga acgaatcata attagctcta atttgtaaca
    46981 tagcgtttgt atgatcagga taatttccaa taacattaaa ttgaaaggca gcattgatag
    47041 ctaaagaatc ccctacagaa ataccatcaa aattagcagt ggaagaagta atttggatat
    47101 tgggagaatt acttagtaaa gttaatgttc caggagttgt aagatcaagc aatcctacat
    47161 ttttaatata gaagtccatt gttaaagtat caccactatg ataaagacca tcttgatcat
    47221 tgaaactgat atggttacat actatgtatg gcatttgttc agctgtagca gtaattgttg
    47281 tattaaaagg ataaaaatta ggtgcattga tataaatatt atataaacca ggagtaagag
    47341 tagcaaataa atttatctgg atcaaaccac tggcatcaga aatagtttta tatatagtgc
    47401 ttccttcata tttcataata aactccgcat taggggcatt ggtttgaatc tgataattat
    47461 taactcctac taataaattg gctggaagat cagcagtaat ggtctgagga gtatttgtcc
    47521 atatcatcat agacggatca ccaaagagat tggtttcgta tgttacccag tacattacag
    47581 gttcattagt gataaaagga atattatcaa tcttgctatc aactaacgca taaccaactt
    47641 cttgatagtt ttccccaaaa atggcatcta taaattggcg atgaatatat tgtgaagcac
    47701 catctgtact accttgcatt ccccatccat aacgtgaatg agaaatcatt gctacagggc
    47761 cagttgcgat tgaagtcatt ttttctgtta ttgaatctga tgtatattgt ccgacatttg
    47821 tttctctatt atcaaaagca cctgcataac aaccctgagt aaagtatgtg gaaaaattat
    47881 agtttattcc atcgttagta atagtagaag cgttaacctg attgttagat aaacgcattg
    47941 tgtaagtagt actggaatgt cctaaatgat taaccagatt tggaccctga ctaagcaaag
    48001 gacgaatctg agaaggcccc caggaatcag ctgctccata agttctatcg tacaaagtag
    48061 aaatattcca ggaagcagga actccagttg ttgtataacc attattacta ctactgccaa
    48121 tcatttcatc catataatca cctccccaag tgggaccatc ccaaagccat tctccaacaa
    48181 ataaggcagt ttttatccca ttagtaattg ggttattggt atagctatta agtttattta
    48241 agaaattagt aatttctgaa tcatcattat agcaaaagcg tccgattgct aattcgggag
    48301 cgagatctgt ttcatacatt tctccccaat agctatctcc atcatcattc catgttccat
    48361 ctaagcaagc ataatacata tcagcaggaa tatcagcatc ggtatattca tcaccatcac
    48421 ttacagatac ataaaaaccc cgatgaggaa taacatctgt atcaccagcc agaaaaacat
    48481 agcggagagg attggttgta taaagtgaaa tgatataatt gcgtagttta tcttgggtat
    48541 caatacctat tgtattgttc gtaatatctg tcatcgtttt cattaaaaca tttctacctc
    48601 ttgcttcctg aaaggttttg aatggtgtcc attgcatttc cttagaggca tctatcacaa
    48661 ttaaatactc aactccagca tcacgagttt gattcattat aattgtttcc gggttatcca
    48721 cagctttttg taaacgatta accgtataag catcattttt caaaaagcgc atcgcatcca
    48781 tagctttaga actagtactg taagaaatat caacactcag attgcgataa aaaacaagtt
    48841 cattctttaa tggacaatat tcaaaaggac taattgctga cattgcaata ggatgaccgc
    48901 ataaaaattc agttcttact ccattatccg gattatcagg atatatcgca gacgaattat
    48961 aaacttcaag atttggtgca tctggagttt caattatctt atgagaaaaa ggatattgtt
    49021 tctgaatagg ggatactcgt ttgggaaggc tgtatacaac tggatcggtt ttggaaacta
    49081 caattttatc tgcttccata ccttcaggca ataaaatttt tattccaaac cagggcagat
    49141 caggatttcc tggttgtccc cagctttgag tattttctaa ctttattttt gtgtattgtc
    49201 catttgagtc aatcttgggc atttctaagg aataattcac tgatactcca gctattagaa
    49261 ctgcagtaga aaacactagt aatactaata atgcgatgtt tttcatttta tgctaccctt
    49321 tttgttcttt gatttttctt cttaatatat tatgcaaggt ctgttccatt ctcgggaaat
    49381 aatacacatt tctctatcat ttggctaata ttattctaac aaatcattaa cgattattta
    49441 atctcgttag agaatcgcct aagagaaatt gaatcatact aaaaaagttg acaccggatc
    49501 ccaaatcaag aaaaagggaa gaagattaaa aaggcatctc acagaagagt gtataatcac
    49561 tggtggaggg atatcaacct tcaacctaga attcctgctt gacaaaaaag tgcttttttc
    49621 aggggtaggt aatatgaaaa tacataaagc gatagccctt ttttctggtg gcttagatag
    49681 tatcatagcc gttaaatgga tgcaaaatag aggatattct gtgcattcgg ttttttttaa
    49741 agctccatat atttttccgg agcgagcgct tcattatgct tccaaaaata acatagaatt
    49801 ggaagttata gatatttcag aagatcatat taagctgttg gataatccgg tatatggttt
    49861 tggaaagtat tttaatcctt gtattgattg tcatggattg atgtttaaaa aagccgcaga
    49921 attattacct tctcgcaatg cagattacat cattagtggt gaagttttag gtcaacgacc
    49981 caaaagtcag aggcatgatg ccttagatag tgttggcaga ttaagcggcg ttaaagattt
    50041 aattattagg ccattaagtc aaaatttact ttctgatacc ttacctctca gagaaggttg
    50101 gattgataaa caagatatgc tatctattca tggacgcgga agatacgctc aattggcact
    50161 cgctgaacaa ttaggaatag ttgattttcc ttctcctgcg ggaggatgtt tattaacaga
    50221 ccgtaattat tgtttacgca taaaagacct gaagaaacat caacaggata ccttggagaa
    50281 tattgaactt cttgcctggg gtcgtcactt tcgtttagat aatgaaacaa agctgattat
    50341 tggtagagat gaagcagaaa atgaggtatt agaaaacatg tttagaaatg gaacaaaact
    50401 gcaggcacga gattatatgg ggcctgtagg atttataact ggaaatgcta gctatgaaac
    50461 attaagttta gccttgagta tatttttgtt atatcatcct aaggcaccag aaaatgctgc
    50521 tattgagatt tggaaagtta tagataagca gcttactcag catactgaaa aaattgccac
    50581 aaaagctaag aaagagttaa ataaagatta cctcatatca tattaataaa agaggtcaga
    50641 agatgttaaa aattgaaagt actgttctac tggaaagtta tcaaaccaac acttatctgc
    50701 tttgggaaac tgacagttca aatgcatttt taatcgatcc tgccaatccc agtgaagcat
    50761 ttttaaagcg tattagacaa ctcgagctga aagttaaaat gattataaat actcatggcc
    50821 atgctgatca tattggggga aaccactttt ttgcgaaagc tctaaattgt cctgttgcca
    50881 ttcatagttt ggatgcggaa atgcttacag ataatagcaa aaattttagt atttatatgg
    50941 gttttgaatt aagtttattt ggcccacaag ttattttgaa tgatggcgat gaactgtttc
    51001 ttggaacgga taaagtgaaa gttattcata ctccgggtca tactaaaggt agtatatgtc
    51061 ttttagcaga taaatatcta atcagtggag atactttatt tgaacaaagc attggtagga
    51121 ccgattttcc tggtggaagt catacagcca taattgattc tataaagaat aaactcttta
    51181 tattagatga cgaagtttta gtttttcctg gtcatgggtt tacaacttcc attggtttgg
    51241 aaaagaaaaa taatcctttt atttattagg agtagcaatg cacaataaag ttttaatatt
    51301 agatttcggt tctcaataca cccaattgat agccagaaaa atacgttccc tgaacatcta
    51361 ctgtgagatt catcctttca caataagtgc agataaacta tattcctttg cccctaaagc
    51421 gatcatatta tccggaagtc catatagcat aaataataac gaaactccta aaccagatga
    51481 atgtatatgg caacttggaa tacccatttt gggaatttgt tatggtatgc aattgatggt
    51541 cgcacacttt ggaggaaaag ttcaaccttc tgctaaaaga gaatatggtt tagcatatgt
    51601 taaaattatt gaccataatg aattacttgc tgatttagga aatagggaag aggtctggat
    51661 gagtcatagt gatgctattt ccaatttgcc tgaatgcttt tctgttttag ctgaaacaga
    51721 gaactctccc tatgccgcta ttgctcataa tgaattacct ttttatgcat tacaattcca
    51781 tccggaagta gtacatacta aaaatgggat caagatttta gaaaattttc tctataggat
    51841 agtaaatcta actcctgatt ggacagctgg aacttttatt aataaaagca ttgaaaaaat
    51901 caggagccag gttggtgata aaaaagttat tttgggctta agtggtgggg ttgattctac
    51961 ggttactgct gctttacttt atagagcaat cggaaaacaa ctattaccca ttttcgtaga
    52021 tacaggttta atgcgttaca gggaagcaga tagagtaaaa gaaacctttt cagcttatcc
    52081 agatttaaat atccgttttg tagatgcggg ggaaacattt ttaaataaat taaaaggtat
    52141 tacggatcct gaaacaaaac gaaaaataat cggaggaact tttattgaag tctttgataa
    52201 agttgccagt gaatttgaag atgttcattt tcttgctcaa gggaccttat atcccgatgt
    52261 aattgaaagt gcatctttga caggaccttc tgctacaatc aaaagtcatc ataatgtagg
    52321 gggcttgcct gataatatgc tcttggtatt aattgaaccc ttaagagaac ttttcaagga
    52381 tgaagttcgc gaagtaggga ccctgcttgg tttacctgaa tctattgttc aaagacatcc
    52441 gtttccaggt cctggtttgg ccgtaagaat tttgggagaa gtagatgccc aaaaagttaa
    52501 catcttgcag cttgctgatg aaatctttat aagtgaatta caagaaaatg gactttataa
    52561 taaaacctgg caggcatttg tagttttatt accagttcga actgttggag ttatgggaga
    52621 tgaaagaagc tatgaacaag tttgtgctct tagagctgtg gatagtgttg atggcatgac
    52681 agctactttc tctcagcttc catttcattt tttgcaaaaa gttgccaatc gtatttgtaa
    52741 cgaagtaaaa ggtatcagta gagtagttta tgatttatct tccaaaccac ctgctacaat
    52801 tgaatgggaa taaaatattc ttatgctaac tgcaatgaat gctatgcgtt tatggataaa
    52861 gtggcgaatt cagcaaaact tttatcttga cagaaaaagc cattttttcc atttgaattt
    52921 ttataatgct tatttgaatg cgctgggggc gtagttcagt ttggttagaa cgtctgcctg
    52981 tcacgcagaa
//
LOCUS       DAOSTH010000008        50684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_760_length_50684_cov_12.700725, whole genome shotgun sequence.
ACCESSION   DAOSTH010000008 DAOSTH010000000
VERSION     DAOSTH010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 50684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50684
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_760_length_50684_cov_12.700725"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            446..913
                     /locus_tag="PLB65_02545"
     CDS             446..913
                     /locus_tag="PLB65_02545"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOQ77435.1"
                     /translation="MTLEDSYRMVIAAEIIAQKMYQALEKSFAKPETSSVFKELVILE
                     KMHEEKMRLVFAKQFPGQEIVLSEEPVKIIPKVDLNDPKEVLDFAISKEEESIELYKS
                     MAGQTTEPEIKEQLLQFANEEEGHKSILLTEIQRLQGALEWFDPSELTGLMED"
     gene            923..2236
                     /locus_tag="PLB65_02550"
     CDS             923..2236
                     /locus_tag="PLB65_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HOQ77436.1"
                     /translation="MVNKDQTTLFDRDELSNKTVPLAEKLRPKTLEELLGQTKLIAGN
                     SPLRQMIESGEYHSFILWGPPGSGKTTIARIIEQNSDYHFIHFSAVLASISDVKAVMK
                     EADYLHRTQNHRSILFIDEIHRFNKSQQDAFLPYVESGAIILIGATTENPSFEVIPAL
                     QSRCTVFVLEPLSESDLKIILQRGFAELKLEEDEDIISWMAQNAGGDARRALNDLELV
                     LPFMKKKPHPSIDELAKFLAKKTMFYDKNREEHYNLISALHKSLRGSDPQAGLYWLAR
                     MLEAGEDPRYIVRRLIRFASEDIGLADPNALVQAIAAKEAILFIGMPEASNALAQLVV
                     YLATAPKSNSLYTAYAEAAEDAKKTSHYGVPLHIRNAPTKLMKDLNYGKNYHYDHEYE
                     NKYYYQKYFPDQMPEKIYYKPGDYGFEKEIQKRIDWWAKLKKEQK"
     gene            2566..2694
                     /locus_tag="PLB65_02555"
     CDS             2566..2694
                     /locus_tag="PLB65_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77437.1"
                     /translation="MKIANPAVILADVFVENNTHIVEFDLQLLVDCSILSLCSANS"
     gene            complement(3052..4653)
                     /locus_tag="PLB65_02560"
     CDS             complement(3052..4653)
                     /locus_tag="PLB65_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOQ77438.1"
                     /translation="MIKPKQKLALISIISIWVLSALLLIVATNAYAQNRPQSTNLYSQ
                     VQLFSEVLYKLKQYYVTDLNDEELIKAAIEGMLGSTDPHTTYFTPEEFKEFTTSTKGA
                     FGGLGIQIDKIGDYITVVSPIEGTPAYRMGITAGDKIVKVNGESMVGLSTDEAIKRMR
                     GEVGTKVQITISRPGLAEPIDFEITRETIKIKSVPYSFKLDNGVGYIRLTQFSENTLS
                     ELRAALNNLESEGIRGLIIDLRWNPGGLLDQAVDTVNEFIGQNRLVVETKGRTENKQL
                     YTRYAVKERNYPVIVLVNEASASASEIFAGSLQDWDKGLVMGKNTFGKGSVQQLIPLS
                     NGNGIKITTAYYYIKSGRCIHKMSNDNILKGKEVSTADLEQESELNRKQVYYTANNRK
                     VYGGGGIQPDIVVESDFLTLFGVELRRKNVFFNFAVDYLVAHNHQFDEKAEISDKTMN
                     DFLNYASKNGIKYTQADVDSADSFIRTTIKSELVRKVKGDAEAYKITISQDKQLMEAI
                     ALFDRFKTLDQMFSYAASLNGKKEK"
     gene            complement(4646..5338)
                     /gene="tmk"
                     /locus_tag="PLB65_02565"
     CDS             complement(4646..5338)
                     /gene="tmk"
                     /locus_tag="PLB65_02565"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOQ77439.1"
                     /translation="MTQSFKKSASHSESYHRESSLPHGLFITFEGIEGSGKTTQIQML
                     EEVLKKKSIPYITTREPGGTKIAEAIRHILLNPDLKEMRPETELLLYCASRAQHTAEL
                     ILPALAEGKIVLCDRYFDSTYAYQGAARDLDEELINVLTKFSTYGRNPDLTFLLDLPV
                     EIGLARIKNRNLDRLEQETISFHEKVREQYLSIARKYHSRYIVLKGESQPEEIHRQIL
                     ASLHTYIGEQND"
     gene            complement(5322..6929)
                     /gene="nadB"
                     /locus_tag="PLB65_02570"
     CDS             complement(5322..6929)
                     /gene="nadB"
                     /locus_tag="PLB65_02570"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOQ77440.1"
                     /translation="MAMIYEYDYLVIGSGIAGLVYALQVSKYGKVAVVTKSSINDCNT
                     DHAQGGIAAAIDTLDSFEAHIEDTYQAGAGLGKRKVISEIISSGPKLIQYLIDLGTDF
                     TRRDPNYDICLENLSLTMEGGHTRRRIAYAADSTGHQIMETLIAQCISNPNIQIYEYC
                     IAVDLITQHHIVQDEGFIPGISCWGAYILDIEDNRVDIIKSRKTMLATGGAAQIYAPD
                     TNPKVATGDGMAMARLAGARLANMEFVQFHPTAFWSPDGETFLISESLRGEGAVLRLL
                     DGSTFMEKYHPQGNLAPRDIVSRAIDTELKKRGDKYCYLDATNVPAEHLLRHFPSINN
                     MLKAKGIDFTKDPIPVAPAAHYFCGGVLSTIEGITDIHNLFVAGEVACCGLHGANRLA
                     SNSLLEALVMAYKAGNHPSNLEEVSFPSIPEWELIGEFNENEWVVISHNREIIGTIMQ
                     GYAGIRRSRRLLKYALSRLENIYNEINNFYQHNSVRKEVVETRNMAIIAISVVRSALM
                     RKESRGAHFLVDNPERDDEHYKHDTII"
     gene            complement(6913..7359)
                     /gene="rimI"
                     /locus_tag="PLB65_02575"
     CDS             complement(6913..7359)
                     /gene="rimI"
                     /locus_tag="PLB65_02575"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOQ77441.1"
                     /translation="MDASYSIRLAKDEDLDSIKKIEQEAFSDPWSDEAFANLTNSWSF
                     VLIKDEEVIGYIFYYGVKEEKSILNFAVRSDFQGMGLGEFLLTETMQIMHNDGVKLLF
                     LEVRVSNFKARNLYKKVGFQEIGIRKNYYRIPEEDAVVMVYNGYDL"
     gene            complement(7378..9027)
                     /gene="argS"
                     /locus_tag="PLB65_02580"
     CDS             complement(7378..9027)
                     /gene="argS"
                     /locus_tag="PLB65_02580"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOQ77442.1"
                     /translation="MIKQLLSNNIIEALDTLKLSHEKEFTVEIPNNPEFGDYSTNAAL
                     VLGKENKQNPMQLAEKIVKELKKNKAYKKVEIAKPGFINFHLAPSLYQQVFWEIYKNQ
                     EYGSSDFGNGEKVLLEFVSANPTGPLNIVSARAAAFGDTIYRIMKYVGYNPAREFYIN
                     DAGNQVDILAESLELRLREIHGEKIGEFPYEAYHGEYVKHLAHRLNALEGTRVFMMPE
                     KDRLEHLKEFALSELLEMQRNSLEKFGVVFEGWVSEKTLRSEGVVEEVLSYLTEADCT
                     YEKEDAIWFCSTKYGDDKDRVLMKSDGSITYFVPDVAYHLTKIQRGFTKLIDIFGPDH
                     HGYVPRIRAAFRALNYDDSILEFIFLQQVNIFESGERVKMSKRAGKIVTMDELISVVG
                     KDAARYFFIARKANAHLNFDLELALKKNNENPVYYCQYAHARICNIIKKARSEKLFPK
                     HFTKDMANKLTKPEELALIQKMADLPELLILIAVHREPHRLTTYLEELASLLHHYYER
                     YQIVNPKSPALTHSRLMLLFTVKKVMGICFELMGISAPEKM"
     gene            complement(9039..9374)
                     /gene="rsfS"
                     /locus_tag="PLB65_02585"
     CDS             complement(9039..9374)
                     /gene="rsfS"
                     /locus_tag="PLB65_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOQ77443.1"
                     /translation="MENNKLTAVISWLEEKKAENIRIYDVEEKSDYTDTIVVCEGTAD
                     IHNKAIASYLMEMAKKNHLQLLSKEGLEYGHWVLIDIGELIVHIFLPETREYYHIDDL
                     LTKFVNKTS"
     gene            complement(9374..9838)
                     /locus_tag="PLB65_02590"
     CDS             complement(9374..9838)
                     /locus_tag="PLB65_02590"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HOQ77444.1"
                     /translation="MNLTNNPPENNLPPRRKIWFPIILILVATAAVVLFFGLRNKAIE
                     PKTGYEEDQLPSIKIVVTNGCGYEHLAADFASALKDKNIEVVSLSQTPKPIYDKTIIV
                     IRKGDMQDLERLKKMTGIQRWTSAYNEYYSADFEIIVGRDYEQFIPKKGRRD"
     gene            complement(9835..10371)
                     /locus_tag="PLB65_02595"
     CDS             complement(9835..10371)
                     /locus_tag="PLB65_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HOQ77445.1"
                     /translation="MNNQNDNHMDKHYLYSPWRLDYILGEKTDECIFCRCQNLDNDKE
                     NLIVYRAKNCYVMLNRYPYNNGHLMIIPYQHSASITDLDDKIWLEMCSLLKDTEQILT
                     RVYNCDGINIGINLGCAAGAGIAEHIHIHIVPRWNGDSNFMSVIGGERVIPESFENAY
                     NKLAPEFANLIKNKEING"
     gene            complement(10364..11392)
                     /locus_tag="PLB65_02600"
     CDS             complement(10364..11392)
                     /locus_tag="PLB65_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOQ77446.1"
                     /translation="MNDLAAIILAAGKGTRMKSERAKVTMPLADKPMIQRVVNTALAT
                     KCNKVFIVVGYMKNNVIAALEDNERIEFVEQQEQLGTGNAVIVTEPMFTNYDQDVLIL
                     CGDVPLLTADTLIRLYEKHKEEQASCTILTAFLDDPGKYGRILRNNEGEICGIKEFKD
                     ATDEQRKIKEWNTGIYCFKAKDLFTSLKQTSNNNHQGEYYLTDTVEILSRAGKKISNV
                     VLEDLMEVSGVNSQEELAALEDIYIGRIRKKWLNNGVIIHNPASVYIGDDVIIEPDVE
                     IYQNTVIKGKCTLEKGCIIGPCCLLENSDVSTEAIMQGYNVLVNAVLPEHHILHYGSH
                     IIEETQYE"
     gene            complement(11385..11888)
                     /gene="folK"
                     /locus_tag="PLB65_02605"
     CDS             complement(11385..11888)
                     /gene="folK"
                     /locus_tag="PLB65_02605"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOQ77447.1"
                     /translation="MIYHLCLGSNLDDPNSQLDKAIYQLSTESGISILRKSSRIITKP
                     YGMLNQPDFVNQVLEIESNDNPQDLMENLLYIEMSMGRLRRIKWGPRNIDIDILLAGD
                     LVLDSRKSPLKKPWPQVIIPHPDLQNREFILRLLNELIPDAIHPVLHKTINELYYELL
                     AREEHNE"
     gene            complement(11891..12268)
                     /gene="folB"
                     /locus_tag="PLB65_02610"
     CDS             complement(11891..12268)
                     /gene="folB"
                     /locus_tag="PLB65_02610"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOQ77448.1"
                     /translation="MKIKLNEMVFYGYHGVQEEERTLGQRFIVSVTLYTNSSIDKGIR
                     RLEDTIDYTKVYLEIKEIMESQQFYLLENCANTIADKLLADFPLLEKLTICIHKPSVP
                     IQGNLKSVEVILSRDRNNQKDNA"
     gene            complement(12265..12675)
                     /locus_tag="PLB65_02615"
     CDS             complement(12265..12675)
                     /locus_tag="PLB65_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HOQ77449.1"
                     /translation="MNAEIIEDSGIVTKVEGNCVTVEIKPGGGCKSCTMRGFCFKESE
                     PTKFEIITDLPLQVNDRVQLDISPVGRTLVSLLVFGVPIIFLFLGFIIASLWFIEFVS
                     ILIGFVAMGLSFRIVRLIDKKYGKRLKISVARKL"
     gene            complement(12672..13157)
                     /gene="rfaE2"
                     /locus_tag="PLB65_02620"
     CDS             complement(12672..13157)
                     /gene="rfaE2"
                     /locus_tag="PLB65_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HOQ77450.1"
                     /translation="MLHTKVIPYNQIAELSENLHKQGKRIIFTNGCFDILHLGHILYL
                     EKAKAMGDVLIVGVNSDASVKRLKGNERPIIPENERALVLAALEMVNYVCIFEQDTPY
                     ELIDLIKPNVLVKGGDWPIEKIVGADIVQKAGGKVYSLNFIAGLSSTNIIERIKQGTQ
                     Q"
     gene            complement(13144..14136)
                     /gene="rfaE1"
                     /locus_tag="PLB65_02625"
     CDS             complement(13144..14136)
                     /gene="rfaE1"
                     /locus_tag="PLB65_02625"
                     /inference="COORDINATES: protein motif:HMM:TIGR02198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HOQ77451.1"
                     /translation="MHSIIPEKLEEILESFKHRHIIVLGDIMLDEYLWGKVQRISPEA
                     PVPIIEVNSADYRLGGAANAALNLKVLGAQVSLVGVCGKDTQATTIITQLKDKEMSTS
                     GIFLDSSRPTTLKTRIGSVSQQIVRLDREDVSELDENIQKEIYAYLTKIMPSCDALLI
                     EDYNKGLLSFDLINDVLQLARKHNKLVAVDPKYLNFSSYGGVEIFKPNYRELESYYGR
                     IFTDEDDFERSAEELRQKMNVKYLVVTKGSDGMYLFTGNKAVKHLPSFAREVYDVSGA
                     GDTVISALTLAYLFNSDIESAALIANHSAAVAVAKKGTASVTTKEILDSFNAPY"
     gene            complement(14117..15505)
                     /gene="purF"
                     /locus_tag="PLB65_02630"
     CDS             complement(14117..15505)
                     /gene="purF"
                     /locus_tag="PLB65_02630"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOQ77452.1"
                     /translation="MCGIIGVYGNNNAARVAAVGLFAEQHRGQESCGMAVGDGFLIRL
                     HKKMGLVKEVFHNEQLSALPGNIAIGHVRYPTKGSATEFNTQPHLVETLSGPCYALAS
                     NGDIVNYAQVRKALEAEKVYFKSDNDGELLVKYIAYRLLHYQEEITDAIRHLMKDILG
                     AYSSVLCTPTELYMFRDPYSIRPMTWGKTPEGTIIVASESCALDTLGVTDRQEVPPAG
                     IIKVNEKGVQILENDPNLYRINKCEKHCIFEQIYFSRPDSFHFGENVYQVREKIGAAL
                     ARQDEGLNPDLVVPVPDSSNFIGLGYANQSGIPLSLGLIRNHYIGRTFIKPEQTVRDE
                     SVRQKFNVLPNFFNGKRIVLIDDSIVRGTTIRKIVDLIKEAGAAEIHLRIGSPQIKHS
                     CFYGIDTPTKHELVANRRTLDEIRDITGVDTLVHLSLRDLQSCVHCPEHYCYACFDGN
                     YPIGVPDALNYT"
     gene            complement(15514..16146)
                     /gene="plsY"
                     /locus_tag="PLB65_02635"
     CDS             complement(15514..16146)
                     /gene="plsY"
                     /locus_tag="PLB65_02635"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOQ77453.1"
                     /translation="MNYPKFLPWIFLIAAYLIGSIPNGLIVSKLLHRKDIRKEGSGNI
                     GATNALRAFGTVTGLFVLLLDVAKGYFPTLLAMSILGKDNSFVSIIALFVILGHMFPV
                     YLKFKGGKGVATAAGVFLAIAPISLSMAILVFLIVVVLFHYVSLASICAAMAFHTHSL
                     AILLKAEKADYATLLLISVAVLLIVVKHKSNLERLINGSESKLKFTKKGN"
     gene            complement(16147..17484)
                     /gene="der"
                     /locus_tag="PLB65_02640"
     CDS             complement(16147..17484)
                     /gene="der"
                     /locus_tag="PLB65_02640"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOQ77454.1"
                     /translation="MRKYIVSIVGRPNVGKSTLFNRLCRKRIAIVDFEAGVTRDRKYE
                     DVQWNGKEFKLVDTGGIVFDSTETIDKMVKHQALLAIEESDLIIFMVDAQTGATDIDR
                     DIAKILYPHRDKVMLVANKADNEKYEWELYDFLQLGLGDAFPLSASHGRNTGDFLDAL
                     IERIPGTQNAYQSEDKPSATKIAVVGKPNVGKSSIANLLLGNPKLIVTDIPGTTRDSI
                     DSPFRYHNKDYVLIDTAGLRRKTRVNYGVEYFSNLRTIEAVDRADIVVLVLAADEELS
                     VQDIKIASYAKRKMKEILVVYNKWDLVEKETNTVNMYLQNLHTQMPFLQFAPVLFISA
                     KTSQRINRIMETVTKIQEESEKRIPTSELNRFMETVVMHRPPTHPSGKHIKIFYITQA
                     QIKPPTFIFFCNTPSLITENYRRFLHNQLREAFKFEGVSIKLIFKGRKKEEED"
     gene            complement(17529..17897)
                     /locus_tag="PLB65_02645"
     CDS             complement(17529..17897)
                     /locus_tag="PLB65_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOQ77455.1"
                     /translation="METYKDLEAWKNGIDLAVMVYKLTSELPESHSFSLATDMLQTSI
                     AVPSNIAMSVQIEDHNIDIEYLEIAFMKLRELETQLIVANDISYLKAEQTDKCFALIN
                     LIRSQISGYFNDHKVNERTV"
     gene            18228..18776
                     /locus_tag="PLB65_02650"
     CDS             18228..18776
                     /locus_tag="PLB65_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOQ77456.1"
                     /translation="MKKLSSANYKDVNYCLRCGHKLELKTDSENKLRPHCSACNWVYY
                     KNPIPAVAIVLFNENNELLLVKRRNEPKAGFWALPSGYMEINLTPEENAIQELEEETG
                     LKGKIMHCVGWYYGDSPIYERILSIGFRMQVIGGKLQAGDDAEEAKFYPLYNLPVIAF
                     DAHRDFISKEIATHQVNLEACR"
     gene            18873..21242
                     /gene="priA"
                     /locus_tag="PLB65_02655"
     CDS             18873..21242
                     /gene="priA"
                     /locus_tag="PLB65_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOQ77457.1"
                     /translation="MYYYVIHLPLDIPKELVYCSSTEIVEGSRVLVNLSGRFWTGICG
                     RKTVPEEGKDIRYKIVTEVLDTSPVLSPELMKLGYWVAEYYHCAVGKALFAMLPSRLI
                     PEMEATITWLAQEIPESFISLKSALDKSENANFKNLRKLLPSYPLYKKIEEGEELGLL
                     KIERKLNHKDKPRTVNFIIRLEPILAEDILPLKQREAWERIKQEELSFPMANISSTIS
                     YSAIKALVKKGFIKIEPRRFDPESISFESSSSPKQIELNEGQLEAIKDILQGFGSFNV
                     NLLYGITGSGKTEVYIELIRYYLNKGKTVIFLIPEIALTPQMVDRFQSSFGQILAIQH
                     SQLTEKDRLIQWQKIKSGKKRIVIGARSAIFAPLPDLGLIIVDEEHESTYKQDSAPRY
                     QGRDLSVVRGQIQNAQVILGSATPALESWNNALSGKYKLQKLESRPLDYSLPEVQLVD
                     LRDEYDQSLISPILLEAIDKRLQKKEQVILFQNRRGYSSFMQCLKCGKLITCTNCEIS
                     MSYHRDREEMQCHYCGNTIPSPRKCPSCGSYSFAYGAPGTQKIEQNLKILFPEAKILR
                     MDSDSARKKDTYKSMYNRMKKREIDILLGTQMISKGLDFPLVTLVGIIMADISLNLPD
                     FRAAERTFQLLTQVAGRSGRSDKAGEVIIQTYNPDHYAIVSASQQNYINFATEELGYR
                     KKLYYPPYYRLARILFQSTDSELLKNEMAAMQETIYQLHKQYPVPQLLLLGPSVAPFS
                     KISSYYRYHIIFKAASVKILQSAIKMFNSLYTCPHHIKKQIDIDPNSLM"
     gene            complement(21436..22221)
                     /locus_tag="PLB65_02660"
     CDS             complement(21436..22221)
                     /locus_tag="PLB65_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ77458.1"
                     /translation="MFQTAIIASGSKGNCVLIQTENTALLLDAGISMHRILECLNFYN
                     IDLSKITGILVSHEHSDHIRSVGSISRKLKVPIYINRPTLSRCEQRLGNINDRIVIFN
                     TGSGFEVGDICVEAFISSHDAAESCNFVFSSVSEPNKKLGVATDLGYPTQLSVLKLSG
                     VSTLILESNHDERMLMEGPYDWNLKKRIHGQQGHLSNIQAVGLISNLIHPGLKNLILA
                     HLSEINNLPSLAEKTMKDYLESIRSDINLIVAEQNRPSPIIEI"
     gene            complement(22227..23180)
                     /locus_tag="PLB65_02665"
     CDS             complement(22227..23180)
                     /locus_tag="PLB65_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ77459.1"
                     /translation="MTDRLTISELHQKVNQEISGLFLVAEKELREGKNDFYLRLKLQD
                     RSGSISANVWKDAAKLSEQFDAGDIINIQGTVVNYKGQTQLSVSKLRFADRSEYNIED
                     YLMRSKIAPEVLSERFFEFVDKVKQPYLNRLLHLIFDDKDFFTQFLTSPAAKSWHHNY
                     IHGLIEHSVSVAALCEFASTLYPVDYDLLISGALLHDMAKIKEYDSKSNIDFTDIGRL
                     IGHLTLSDQIVCETASKIAGFPEELLLNLRHLILSHHGEYEKASVRLPQTLEAVVLHH
                     CDNLDAQTVGVAQLIESAPKNAIWTEYDKLNNRYYKIYRPV"
     gene            complement(23189..25138)
                     /locus_tag="PLB65_02670"
     CDS             complement(23189..25138)
                     /locus_tag="PLB65_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77460.1"
                     /translation="MKLFITSSGYYISGKQKIINFLVHLIILFFLMLIPIMSNAISPL
                     QVVPGSGIKDIEDSKIYQFRYHNLADDYHLSGPTIWAVRFNFKAAYPLADSATFSLNS
                     FKIYNPYQNMEMRVSLWNEQYAENNDLIYFPGSIIPGGDWQNITLFTNPTNIVFPEIQ
                     NPLQVVWLVMELTTPITGKYISASEGSGKNSFYYNTTVHGNEYWQSLFSAGFACEIRI
                     SAVGDFNLLNTDLELLNFTLPENILPSSTVYPSVTVYNHSNFPITDTLSVHFSDPMLQ
                     LDQYLLIPLQNINARDSLRVVSSEGITFNREPTEVKVNLAFKNHPSVVLPARYYNVFM
                     ETGSCHLIEYFRRYTFETDDIPRDDSGLHHLLYIPNQADYLSCLGASQRFNFYQMNSL
                     PQTVVDGYKRFHLPLAADSEQILTAISSAQEKRSFISSSETRINILDPDNPENLLLNI
                     TLSNDSTTLFEWSSTSAIYPKFFAAIFEENNFENRSLYTLKKWLVYNEPISSTINLHS
                     SMEIDLIINTSELDSLKSYRVYYWLQNSYTNGGQVFYAKCATLPYGWTVANSDDNLPR
                     PNLKIYPNPLNNGQSLKISGFTSPSVFTVYNLKGQKIFTTGLINAEVNVPFEVFPASG
                     IYFVQSETSERGKAIKQIKKISIIK"
     gene            complement(25135..26040)
                     /locus_tag="PLB65_02675"
     CDS             complement(25135..26040)
                     /locus_tag="PLB65_02675"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOQ77461.1"
                     /translation="MNTNSVLTIGTFDGLHLGHQKLLKKVVEIAQAEKLQSVVISYKD
                     HPAFILRAKALPKMLCPITLKQRELAKIGIDRIELLDFTPELAKTPPLKFLKEYVIPT
                     FHPKVIVMGYDSHFGHLRQGNYEFLCRHSQELGYRVEYLEPLLYEGKPISSSTIRNLL
                     SLGKIETANNLLGRAYCLIGTVIHSYSNGRKIGFPTANLKLLYPHQLIPKNGIYLSRI
                     RYQEKVYFGLTNIGISPTLKNSGIIEIETHILDFSGDLYGETLEIELLRYFREEKMFK
                     KVDELKDAIQKDIAMARTIISELDK"
     gene            complement(26194..27066)
                     /gene="truB"
                     /locus_tag="PLB65_02680"
     CDS             complement(26194..27066)
                     /gene="truB"
                     /locus_tag="PLB65_02680"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HOQ77462.1"
                     /translation="MPVTVNAFLAINKPAGITSFEVIRRLRRITGIRKIGHTGTLDPF
                     ATGLLICCTGSYTRLSCFLEKEEKTYLATMKLGAKTSTGDTEGEIIQTAPVPIFADKL
                     AALEAKALDLAELSVPAYSAVKIQGKRAYELARQGKILELPMRPVKISEFKFLVEEQN
                     APDELTYRCKVSKGTYIRALSEWLAEQIQTLAYTISLKRESIGKITLQDAVALGDLKT
                     DNWTNSQISYERVFPDWTCLKLNDEELYKVINGMDIEFSESSSDIILLLNQDELCALG
                     KKSNNVIHPFIVLK"
     gene            complement(27050..28045)
                     /locus_tag="PLB65_02685"
     CDS             complement(27050..28045)
                     /locus_tag="PLB65_02685"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HOQ77463.1"
                     /translation="MEKIKIKLLSEFSKAERIVITSHIGLDGDGYCSALALQRIISSL
                     GKNSVIVTDNDDLSRYDYLQDGKVQEKRFSSLNEEERTFSLAIALDCNSYDRLGDRRI
                     LIDNAVTTIVLDHHQQENGLIKADLRYIDPSFACVGELIFSLLEEEINKMPSSDRIFV
                     CNCLYTTILNDTNNFTNANTDAKVLNFAAKLVDLGIQPHTLYKNFYLNQSAEEMRYIG
                     QTLSTIELYHHRQILMMHSTYLMSKENNIDPDSITNVTRWVQGVNGVQVIVYLREDKE
                     GVYKISLRSETIDVNKIAVRYGGGGHKQASGCNMYGDIEEIKAKLLKDIIDACNG"
     gene            complement(28127..28615)
                     /gene="rbfA"
                     /locus_tag="PLB65_02690"
     CDS             complement(28127..28615)
                     /gene="rbfA"
                     /locus_tag="PLB65_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HOQ77464.1"
                     /translation="MKNYRIPRLQSELKKLFNTALSQKLNDPKLAWVQITDVVISKDL
                     HYAKLYFSHYNNPASHEQIRELLMKSSGFFKKQIAGAQIMRTIPELSFYYDETEDRAA
                     KVDLLLDSVKNNFNDDDDDFDDDIDIDDYLDDDDFFEFDDDEDDDDYDDFEDEDEDED
                     EE"
     gene            complement(28615..31179)
                     /gene="infB"
                     /locus_tag="PLB65_02695"
     CDS             complement(28615..31179)
                     /gene="infB"
                     /locus_tag="PLB65_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HOQ77465.1"
                     /translation="MTIRVYDLAKELKISTMALKKHLTDLGVNVKSHMSLIDEEVADK
                     IRIKYKEQVDAEKRAERDRKRLIEMRQAAKAKQETPEPEAKKEPEIIAPVVEQITVVP
                     EPEEVKPIAEPEKKVAKPVQEKAEERTVKPAPQKEKPVSPPKYSKPSKPEVVAPKPST
                     LHKKDAGKTEQYKHKPSAEKGTKPVVVAPVPEPIKEVEERKFGKAKVSHEELGEKSKH
                     KKAIISSSKKAKQKIVDVIEIDEAEISRNIKKTLQKSSKRKKYHREAQTVEERSNEIV
                     IREFTSVSELAKIMNVAPSEIISKFFMMGQLVTMNQRLDKDSLEMICDEFKVDFRFED
                     EYGMDIIGRELEQYSEVKEERRPPVVTIMGHVDHGKTSILDYIRNENIVAGESGGITQ
                     HIGAYQIEINKHKITFIDTPGHEAFTAMRARGANVTDIAVIVVAATEGVKAQTREAID
                     HAKAAGVSIIIAINKVDLPEANVDKVISQLLDMGIYLEQYGGDIPWCRTSVVTGEGIL
                     NLIELILLTAEIMELKAKREVPASAVVIEAGMDTRMGPSATILMQEGTLRKGDIVVCG
                     AVHGRIRKMENERGAEIKVIYPSDVARIYGLSDTPKAGDILNQVDSEKTARSISSERQ
                     QIRLEREKYLNKSSLQNIFARIKEEKINSLNVILKTDTDGSSEALADSFQKLSNEEVS
                     VNIIHKSVGGINEADVSLAAASDAIIIGFNVRASSQVRKFAEDEGVQIKIYQVIYDAI
                     EDLHNALEGMLQPEFEEQVIGSALVKQVFKIKKVGTIAGCQVDKGIIQANCKVRLYRN
                     DVLVTEDTISSLKHYADDVNEVRAGSECGISLANFSDIKEGDILEAFVINEISKKL"
     gene            complement(31176..31505)
                     /locus_tag="PLB65_02700"
     CDS             complement(31176..31505)
                     /locus_tag="PLB65_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HOQ77466.1"
                     /translation="MNSKILTLMQFARKAGKLVVGTDACERAIGRKEVFLLILAQDNS
                     ERTKNKMKHIAGAAKKSLEVIETGTQEEISSALGLSITGVFGIKEKHIAGKIMEYWLS
                     ETERRIS"
     gene            complement(31502..31741)
                     /locus_tag="PLB65_02705"
     CDS             complement(31502..31741)
                     /locus_tag="PLB65_02705"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HOQ77467.1"
                     /translation="MSNKNSKAKHIPIRTCVVCKKKVDQNRLLNFFHTTSGIVFDCNR
                     IIQVRKYYLCPNEECFKGLSKWQKRYQKKSLGSIR"
     gene            complement(31741..32976)
                     /gene="nusA"
                     /locus_tag="PLB65_02710"
     CDS             complement(31741..32976)
                     /gene="nusA"
                     /locus_tag="PLB65_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HOQ77468.1"
                     /translation="MSANMLEALSKLAAIKQLDKEMIQNIILESVISTLQKRLEPESE
                     LEVYIDDLSGNIKVKFQSLVVEREEGLGQISLTEAREEYDPEVQLGQFIEKTMSLYEF
                     EPKLIKTIQKIIQDKIRKLEDDKIQNDFNKQKHTIVTGKIKAIDDFGGYIIDIGYTDA
                     LLPLDEQIENEFYRVGENIKAYVVNIRSGKDGVVIVLSRTNPEFVKKLFEAEIPEIFT
                     GEIKIRKIVREPGIRTKVELETANPKIDPVIACVGPKGTRIDSIRKELHGEQIDIVIH
                     SDDPEKMVENALGVTGIKRVIIERNHSASVIVEESDKVMAIGKQGKNVKLAAKLVGLK
                     IDIYTMPEYEEKMAKERRTISHIAELDGVTPKIAEVLRQAGYTSVQDVYMASIEELRN
                     LEGMGQKTAERLKEAAKYF"
     gene            complement(32980..33450)
                     /gene="rimP"
                     /locus_tag="PLB65_02715"
     CDS             complement(32980..33450)
                     /gene="rimP"
                     /locus_tag="PLB65_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HOQ77469.1"
                     /translation="MEFYRTQIEEIAKKICQEMHLALYDIDEKMTGKGRVITVYLTKI
                     GGVTLDECASFSRALGKELETFDLIPERYFLEVSSPGLERPLKLKSHYVSAISEKVAV
                     QYSEGEEKKSVMGTLIEVNQDTIVLDDRGTIIEIPFKSITRAKTVFLAIPKMRG"
     gene            complement(34307..35059)
                     /gene="phnC"
                     /locus_tag="PLB65_02720"
     CDS             complement(34307..35059)
                     /gene="phnC"
                     /locus_tag="PLB65_02720"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HOQ77470.1"
                     /translation="MNSKNKLLEVINLNKSYDGHILALNNLSLQVQEGDFIILLGLSG
                     SGKSTLLRCINRLIEPSSGHILFREQNILPLKGIELRHYRRQIGMVFQQFNLVKNLTV
                     LTNVLTGRLGYHSSLSTFSKEDYELAMQNLEKVGLQNYANKKVKFLSGGQQQRVGIAR
                     ALMQNPSLILADEPVASLDPATADSIMQYLGEINKDGITIICSLHFLTLARRYGNRVL
                     ALKEGKKVFEGIPAEIDNFRFKEIYGQDAEEI"
     gene            complement(35056..35982)
                     /locus_tag="PLB65_02725"
     CDS             complement(35056..35982)
                     /locus_tag="PLB65_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ77471.1"
                     /translation="MLKRYILLTFIALLILGCGNQESRLEKANRKLGSKQNPIKMFFV
                     PSLEAGKVVSSGEAIAKYLENETKLHFKVAVPTSYAAVIEALGTYQADIAWLPTYAYI
                     LAKQKYDANVQLMTVRNGLHKYRGQFIARSDSDIKSLQDIQGKVIAYTDAASTSGYIY
                     PSAILKQNGITPKDYFFAGGHPQAILAVYSGRADVGCSYWSPSDSAGKPMDAREKLLD
                     TYPDVFEKVKIVGYTDWIPNDTVTFRKDLPPELQGLIVQTLYRYAQSLEGKKVLKTLY
                     DIDGLEYATDEDYEIVRTTLKSMNMDPADLLK"
     gene            complement(36051..37322)
                     /locus_tag="PLB65_02730"
     CDS             complement(36051..37322)
                     /locus_tag="PLB65_02730"
                     /inference="COORDINATES: protein motif:HMM:NF016354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HOQ77472.1"
                     /translation="MSVVIQNILPKSLAAKNGIKSSEILLTVNGQEIRDFLDLELYTS
                     DYEFELEIMTPPGEKRIVKIQREEKSFLGIELEPYNIRSCENSCIFCFIDQMPPHLRN
                     TLYLKDDDYLYSYVFGNYITLTNLTNEDYERIITQRITPLYISLHTTDSTLRQKMMRP
                     LRQVDALSALKNLSQYGISFHIQIVCVPGYNDGEALKQTLKDLHQTKLNCLSIGIVPV
                     GLTKYRQNLCDLKTFNQHNAIEVLDLIEEARNLYSSDIIYPADEFFVLAGRNIPDEDY
                     YADYPQLENGIGMLRLTIQNYKLKKRSILKELRKKPVDYLMISSVCAKEVISKIAEDL
                     NKRLENQRIRVQVINNDFFGPDINVCGLITYSDLYSQLSPEKNETLILPSCIFNTEGE
                     TLDGKDRITFQETWNNPILLIDQFFEEWDYL"
     gene            complement(37342..38463)
                     /locus_tag="PLB65_02735"
     CDS             complement(37342..38463)
                     /locus_tag="PLB65_02735"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOQ77473.1"
                     /translation="MKQKIITILMLISCLGMLFGNINIQIKGETKPHILKETTLASVP
                     YVELEEFCSVFKAVCKQDRTDNRLYLNIYDEQFIFLERSSYYTLKAVSYNMNYPLQRK
                     GERLYLPSTFVNEHLHTHFPAQVKLKGNTLQISKPIDNSVKTIVLDPGHGGKDPGAVG
                     RKLRANEKDINLAVAQKLKVLLEKELGITVLLTREDDRFVSLYDRTRFANEKKADLFI
                     SIHSNSSKSTSSKGIETYYLSTAQTSDARAVEALENAVVERFEGGSEAKKKYDDLDFI
                     LSDLLQTENLEHSNNMATSVHQNLVMGTQGIDRGVRQANFYVLRGAYMPSILVELGFI
                     SNAEEEQLLVNEEYQERLARTIFEGIKRFKFRYDRVKNT"
     gene            complement(38460..39404)
                     /locus_tag="PLB65_02740"
     CDS             complement(38460..39404)
                     /locus_tag="PLB65_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ77474.1"
                     /translation="MPYSSMSSCNICPRNCGIDRTKHIGYCGVTDTLKVDLITRHFGE
                     EPPLTGTRGSGTIFFSGCNLRCVFCQNYDISSNCWGKDITKDELINLMFKLQTEGAHN
                     INLVTPTHFTRQLREAIIYAKKEGLAIPIVWNSSAYEKVETLQSLNGLVDIYLPDFKY
                     AHKVYARKYSSASDYPAIAIAAIKEMFSQVGFLEVDSNGIAKKGIIIRMLVLPYGLAG
                     CKENLYTLANEIGVDLTLSIMGQYYPAGKANDYKELRRGISPKEYQDVIETAVALGFT
                     NIYTQEISGSELWTPDFSPEPNNVYGGSEFNPIKEQTI"
     gene            complement(39405..39806)
                     /gene="cdd"
                     /locus_tag="PLB65_02745"
     CDS             complement(39405..39806)
                     /gene="cdd"
                     /locus_tag="PLB65_02745"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HOQ77475.1"
                     /translation="MLKLKPREKELLEEAKKASANAYSPYSNYKVGCAVRTVDEQIIT
                     GCNVENASYSLTICAERNAIFKAISEGHKDFTEIAVFVDSEESFPPCGACRQVIYEFA
                     PDINVIYANRNTVQIINIKELLPEAFILKRN"
     gene            complement(39817..40917)
                     /locus_tag="PLB65_02750"
     CDS             complement(39817..40917)
                     /locus_tag="PLB65_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOQ77476.1"
                     /translation="METLFTGLLGFQLKQVIMILIGLVLIWLAIKHKYEPTLLLPIGF
                     GTILANIPLSAAVGEHSPLGILFKAGIDTELFPLLIFIAIGAMIDFSPLLKNPFMLLF
                     GAAAQLGIFVTIVLAASLGFDIKEAASIGIIGAADGPTSIYVANRFAPNLLGAISVAA
                     YSYMALVPIIQPPVIRLLTTKKERCIRMDYHSGDVPQTVKIIFPIAITIIAGIFVPAS
                     LALIGFLMFGNLIRESGVLEGLSKTAQNELGNLVTILLGITIASKMQGELFLVPQTLL
                     ILALGLVAFIFDTAGGVLFAKLMNIFKKEKINPMIGACGISAFPMSARVIHQMGLKED
                     PFNFLLMPAVSVNVGGQIGSVIAGGLILALLA"
     gene            complement(41371..44655)
                     /locus_tag="PLB65_02755"
     CDS             complement(41371..44655)
                     /locus_tag="PLB65_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOQ77477.1"
                     /translation="MKKLSILLMLIIAIGVLTAINFDPELFYSKTIIACFTKTAIPNL
                     DGVVNFDVQDGIVSTGMSSFDQLAKELHIVDMKQMHPYVKVPEWNDNGIYLQNHYRLY
                     LESDDNMDKAVELLSKDPNLVYAEFEGINRPKYVTNDPMLDLQYAHTLVHSFEAWDYT
                     LGSHDVLVAITDSGVKWNHPDLRANIWINPAESPGMTIDWNAGTISGGNGVDAGEGGN
                     KVDDLVGWDFYNNDNNPMQNFAANDHGTHVAGCAGAVGDNEIGVVGTCPNVSILCCKG
                     SSNTSPSTGVSYAYDQVKYAGEIGADIINASWGSVGSGTYPNSIVNYVTALGALVVTA
                     AGNENTEHNASYQDYPADCTNALCVAAVAQGDVKASFSDYGSTIDICAPGAAILSTII
                     TGDGYAAYDGTSMASPVAAGVAALVKSLNPNLTPVELMQRLMATADNIDSLNPDYVGL
                     LGAGRINAYTATMYDKIPNMQIEDYILEEISGDDDGVANPGEVIKLRIALSNCIDPIT
                     GLGWLQAENVTVTMRCHYPGVTIIDSVATFGTMHAGTTLLNNNQPFKFQTVSTLPSEP
                     IPFQFYVQANQDNQYPYNKALPIDISLSLNQAGWPFYSGAATNSTPLLIDMDDDGQKE
                     VVFVGADGNIEVLRKDGLTSYPGFPVQTSSVIIGSIAMGNINNDSNREFVACLQNNQV
                     ICFNDLGEVKWNNASGGTLRNGPVIFRPNAASELKVACVTQNGILNIFNGDGSNFTNF
                     PVTVTGAYLAPPAIGDLNNDGYMDIVMIALNGSLNAISSQNGQSLPGFPVSLTGGGSQ
                     NAICIANLDSDPEPEILVASSSGGYLYGVNHNGSVLFQKNIGTQIKTSPVVVDVNNDN
                     IKEIVLIANNGNIYIMDGMGVDLPNTPIGINTAVECTPVIARFDGGNNAGIIFGDSSG
                     KLHSVRIDGTESPNFPIEIGGILKVSPTLADIDGDNDLEIAFPTNSGLSIIDVKRGAQ
                     ALLWPNYLGGFGRTCNAYQPTPVVDPQLPALETVLYEAYPNPFNPETTISFNLSSPNL
                     TELYIYNQKGQKVKTLFSGNLESGNHKFVWNGQDDNGNSVSSGLYFYRMKSGKYSSTK
                     KMILMK"
     gene            complement(44734..46833)
                     /gene="recG"
                     /locus_tag="PLB65_02760"
     CDS             complement(44734..46833)
                     /gene="recG"
                     /locus_tag="PLB65_02760"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HOQ77478.1"
                     /translation="MAESKKTTDLHSEIKYLKGVGENRARLLGKLGIKTILDLMEHFP
                     KLYISRKLSAHLEDLKPGDMLAFTAMISWVDVHTSLKGKKILNIGVSDGKVGLICSWF
                     TYPPVYEKLFIPGRLVWLNGTISEFNGQLQMVHPDFELIDDSEDTKDDFWKKREVLPV
                     YPLTEGISQKLMRQLMYNAFSLYTGLIEENLPEYILTKHHFLERHIALQKMHFGQNPQ
                     EIEIVRRRFAYEDFFYSQLLWARHKLFHTTTTTGINFINKKQLTTALYRKLPFTLTSA
                     QKKVLWEIFNDMCSEKQMSRLLQGDVGSGKTVVSLFAMLLAVENDYQAAMMAPTEILA
                     EQHYETISQLLKDSEIKVCLLKGGVYKGKEETKNAIAEGEAQIVIGTHALIQKDISFK
                     RLGFACVDEQHRFGVEQRARLARQALHPDLLYLSATPIPRSLAMTVYGDLEVSILDEL
                     PPTRKPVRTIIRPSSKIDTVYIEVRKELEKGRQVYIVCPLVEESEKISLLDATKLYEY
                     ISKKIFPEYPACLLYGKMPVKEKDAIMKRFKAGEIKILVSTTVIEVGVDVPNANVMII
                     EHSEHFGLAQLHQLRGRVGRGSEQAYCYLIEHQPVNDVAWQRLTTMTKTTDGFIIAEK
                     DLELRGPGDLFGFEQSGMPQFRFANIVRDQEILKIARQDAFEVVRSDPDFSQIKNAIV
                     KKMYHHQYTKKEELILY"
     gene            47307..49856
                     /locus_tag="PLB65_02765"
     CDS             47307..49856
                     /locus_tag="PLB65_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOQ77479.1"
                     /translation="MKRTVLMALMVGFVMASSLQGENLSASLQTNIETENIAYEDYSP
                     LTEKDIYELKNLLQEIQLNPSDLNFEKDWDLSTWGKSEEFLHALQEPYYGLKKMQEYR
                     NAANKSDTDDGFAFLADLLYKNAIDDSGSISIYREYKQIYQQNFWREVQQPQDLFPFY
                     ETALKDIIPILAEAYANYNSELWSKYISYLFTALIESEDKEAYSTYFNQHNIPETRDV
                     EDCEIKKQMGLLLEPAILKAGMQFLALGDVMKNEAGKLLYKNKKPIIKTGKFGTMIIG
                     TVHDDVYSEKLLKKPLILLIDPSGNDKYEFSLATGYDNYTYLLIDYKGDDSYLNPKIG
                     EMFFALSGLGISYDLQGNDIYKTGDFSFAAMMGMQLHRDFQGNDIYQSGLFSQGAAIR
                     GLSLLQDCQGNDIYNATCLAQGLGSIRSAGAILDMEGADNYLVGGKYFHSPLMPNDYL
                     TLGQGVGFGLRPDFAGGFGLLYDKNGNDRYLGGVYAQGVAYWYALGMLIDEAGNDVYN
                     AIYYPQGSGIHLAAGFLFDGQGDDAYYSRHGPGQGAGHDWGLGILIDAQGNDAYSIEG
                     GNGLGLSNSVGIFVDKSGNDRYERKNSQNYGYAAYSRATGSIGLFLDEGGIDTYPDTL
                     MANGTIWKKGTYAVGKDITQTEEKDDLTKNEMPMLTPPAPDDSIENIFAAAAEWEVGN
                     AIERVKKAREIMLDRAEEASEYILKYKLNTKSGLEYRALEVMVKENESFKQLLFNYTD
                     DADSLKAKNALALIAGVGDSTLIEPIRKHLNNGKYITTCLSLLGSIKSEESVNLLAEY
                     AFHPCERYRYLVARSLKQINTPQAHNYLIKMADDSSFLVKTLIRKFKEEQQ"
     gene            49853..50494
                     /gene="rsmG"
                     /locus_tag="PLB65_02770"
     CDS             49853..50494
                     /gene="rsmG"
                     /locus_tag="PLB65_02770"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOQ77480.1"
                     /translation="MKDEKQIFEDYLNSLELENINQLMKRFEHYHNLLYTTNRKVNLV
                     SRNIPSDRYWVQHFLDSLVALECLDLEGKFVLDFGSGGGLPGIPLKLAVPDFNLTLLE
                     SVQKKSRVLREFVDALSLSATNVEASRLEDYAYVARRPSYDIIVCRAISLEERYISPI
                     RRLLKPSGMVVFYKAQKLDDVQNLHYEVLLDKKYPSLGQRKLIGIKQRDLMLR"
     gene            50518..>50684
                     /locus_tag="PLB65_02775"
     CDS             50518..>50684
                     /locus_tag="PLB65_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ77481.1"
                     /translation="MPNIITVVNQKGGVGKTTTAVNLAAALAVLEKRTLLVDFDPQGN
                     ATSGVGIDKDKV"
BASE COUNT        15636 a         9668 c         8845 g        16535 t
ORIGIN      
        1 atacaacagt gtgttataac ttgtccacat cacttttttg tgggaatgcc ttaaaaatca
       61 gataagtcga agtatcttat caactcactg ccatagttgt tttcttaaga taattaatag
      121 gatcatagca ctgatgtcgg tgatagaata tatcatccat aatatgttac cgtcattatg
      181 aatgcgcatc gttagacaaa atataggttt atcttaaatt atcgtagcga tgataactcc
      241 taactttcca taagattgaa taatattaat gaagctaatg attaattttc gaaatatctt
      301 aaagctcggt tggctggaga acgaagagga catcgttcat cctgcataca gattactgca
      361 ttctgtattt tttcacccct ttcaaccttt tcttgcttta agccacaatt tcgattatta
      421 tattataagc aaggaggata aaaaaatgac attagaagat agttatcgga tggttattgc
      481 tgctgaaatc atagcgcaaa agatgtatca ggctttagag aaaagttttg ccaagccaga
      541 gaccagcagc gtttttaaag agctcgtaat attggaaaaa atgcacgagg aaaaaatgcg
      601 gcttgttttt gctaagcaat ttcctgggca ggagatagtc ctttctgaag agccggtaaa
      661 aataatcccg aaggtagatt tgaatgatcc caaggaagta ctagattttg ccattagcaa
      721 ggaagaggaa tcaatagagc tctataaaag catggcaggc caaactactg agccggaaat
      781 taaagaacaa ttattgcaat ttgccaatga agaagagggg cataaatcta ttttattgac
      841 tgaaattcag cgtttacaag gtgctttgga gtggtttgat ccttctgagt taactggatt
      901 gatggaggat tgaacatatt ttatggtaaa taaagatcag acaaccctgt ttgataggga
      961 tgaactcagc aacaaaactg tacctttggc agaaaaatta cgacctaaaa cgctggaaga
     1021 actattgggt cagacaaaat tgatagcggg gaattcacca ttaaggcaga tgatagaaag
     1081 tggggagtat cattctttta ttttatgggg acctcctgga agtggcaaga caaccatagc
     1141 ccgaataatt gaacaaaaca gcgattatca ttttattcat ttttctgcag tccttgccag
     1201 catcagtgat gtaaaagcag taatgaagga agcagattat cttcatcgaa cacaaaatca
     1261 tcgtagtatt ttgtttattg atgaaattca tcggtttaat aaaagccaac aggatgcctt
     1321 tttaccttat gtagaaagcg gggctattat attaattgga gcaactacag aaaatccttc
     1381 ttttgaagta ataccggctt tacaatcccg atgtaccgtt tttgttttag agcccttaag
     1441 tgaaagtgat ttaaagataa ttttacagcg tggttttgcc gaattgaagc tggaagaaga
     1501 cgaagatatt attagttgga tggctcaaaa tgccggaggc gatgcgcgta gagctctaaa
     1561 cgatttggag ctggttttac cttttatgaa aaagaaaccg catccgagca ttgatgaact
     1621 ggcaaaattt ctggctaaaa aaacaatgtt ttacgataaa aatcgcgaag agcattataa
     1681 cttaatttct gctttgcaca aatcgttacg gggttcagac cctcaagctg gactatactg
     1741 gctggcaaga atgttggaag caggagaaga ccctcgatat attgtccgtc ggctgattcg
     1801 ttttgccagt gaagatattg gcttagcaga tccgaatgcc ttagttcaag caatagcagc
     1861 caaagaagca attcttttca tcggaatgcc agaagcaagc aatgctttag ctcaattggt
     1921 agtatattta gctacagccc caaaaagtaa ctctctttat actgcctatg cggaagcagc
     1981 cgaagatgct aaaaaaacaa gccactacgg agttcccttg catatcagaa acgcgcctac
     2041 aaagctgatg aaagacctga attatggaaa gaactatcat tacgatcacg aatatgaaaa
     2101 taaatattat taccagaagt attttccaga tcaaatgcca gaaaagatat attataaacc
     2161 tggcgattac ggttttgaaa aggaaattca aaaacggatt gactggtggg caaaacttaa
     2221 aaaggaacaa aagtagaaag ctttgtacag tgcaaattta atagtaatta tcaattctac
     2281 ccgataggca ttttgtcgtt gcacatacaa tcaagttttg tagttattga tattatatat
     2341 aggagtgagg aaagtagtta gtgttgctaa tcagattagc tacagaataa aaaacccctt
     2401 cgtttggaag gggtttaggt ctttgactta ggaggtcgca cattaaatga cctttggggg
     2461 aaaggtcatt aaccggcaag ggggcgccgg ttatttgaga ttcattaaat acaagtaagc
     2521 atatcctata ccaaatattg attctctgct atatgtttat taaatatgaa gatagctaat
     2581 cctgctgtta ttttggctga tgtatttgta gaaaacaata cacatattgt tgaatttgat
     2641 ctacagttat tggtagattg cagcattcta tcgttgtgct cagctaatag ttgacttaat
     2701 attttggagc ccaaatatgt ctcctaccta attagctata gtggaactga ggtgaggcta
     2761 cggagcaaaa tcccaaggat gcaccatttg attcccaccg tagctaatta aggaagaatc
     2821 aaactacatt tcgaattttg cttataaggc aaataaggca aagctagtag atgtttagca
     2881 caataagcga aaaggatata caattctaag gcaacaatta cccttcagcg aacttatgtt
     2941 gtttgcatta tcaaccggaa cacaatggaa ttttctattc cattgtgtat tatacttctg
     3001 aagataagct atagatcgct atccaatcag ttatttaagc tgttcggttg tttacttttc
     3061 ctttttccca ttcagggaag ctgcatagga aaacatttga tctaaggttt tgaatcggtc
     3121 aaacagagca atagcttcca ttaactgttt atcttgcgag atggttattt tatatgcttc
     3181 cgcatcgcct ttaactttgc gtacaagttc gcttttgatg gttgttcgga taaaggaatc
     3241 agcgctatct acatctgctt gagtatattt gatgccattt ttggaagcat agttaagaaa
     3301 gtcgttcata gttttatcgc tgatttctgc tttttcgtcg aactggtggt tatgggcaac
     3361 cagataatcc actgcgaaat tgaagaaaac attttttcga cgcaattcta ctccgaaaag
     3421 agtgagaaaa tcgctttcga caacaatatc gggttgaatt ccgccaccgc catatacctt
     3481 tcgattatta gcagtgtaat aaacttgttt acggtttagt tcagattctt gttccaggtc
     3541 tgcggtacta acttctttac ctttcaatat gttatcgttg ctcattttat gtatgcagcg
     3601 tccgcttttg atatagtaat aagcagtagt aattttaata ccatttccat tggaaagagg
     3661 gataagctgt tgtacactgc ctttcccgaa ggtattctta cccataacca aacccttatc
     3721 ccaatcttgt aaacttccgg caaaaatttc ggaagcactt gcagaagctt cattaactaa
     3781 aacgataaca ggataattgc gttctttaac ggcatagcga gtataaagtt gtttattttc
     3841 ggtgcgtcct ttagtttcta caaccaaacg attctgtccg ataaattcgt taactgtatc
     3901 tactgcttgg tctagaaggc caccaggatt ccaacgcagg tctataatta atcctctgat
     3961 gccttcgctt tctaagttat tcaaggcagc gcgcagttcg gaaagtgtat tttcgctaaa
     4021 ttgggttagg cgaatatatc cgactccatt atcaagctta aagctataag gcacgctttt
     4081 gatttttata gtttcgcggg taatttcaaa atctatgggt tcagctaatc caggacggct
     4141 gatagttatt tgaacttttg tgcctacttc tcctctcatt cgtttgatag cttcgtcggt
     4201 agataggcca accatagatt ctccattaac cttcacaatt ttatctccgg cagtaattcc
     4261 catcctgtaa gcaggtgtac cttctatagg agaaaccaca gttatataat caccaatctt
     4321 atcaatttga attcccaatc caccaaaagc acctttagta gaagtagtaa attctttaaa
     4381 ttcttcagga gtgaaatatg tggtatgagg gtctgtagaa cctaacatac cttcaatggc
     4441 agctttaatc agttcttcgt cattcaaatc ggtaacataa tattgtttca gcttataaag
     4501 aacttcactg aaaagctgta cctgagaata taaatttgtg ctttgaggac gattttgggc
     4561 ataagcatta gttgctacaa taagcaacag ggcactaagc acccaaatgc tgattatcga
     4621 gattaatgca agcttttgtt tcggtttaat cattttgttc tcctatatag gtatgcaagg
     4681 aagcaagaat ctgacggtga atttcctcag gttggctttc tcccttaagg acaatatatc
     4741 tggaatggta tttcctggca atagataaat actgttcccg cactttttcg tgaaaactga
     4801 tagtctcctg ttccaaacgg tctaaattgc ggtttttaat tcgggctaaa ccaatttcta
     4861 ctggaaggtc taaaaggaat gttaaatctg ggtttcgacc ataagtggaa aattttgtaa
     4921 gcacattgat taattcttca tccaaatctc ttgcagctcc ttgataagca taagtggaat
     4981 caaaatagcg gtcacacaga acaattttac cttcagcaag agctggcaaa ataagttctg
     5041 cagtgtgttg agctctactg gcgcaataca aaagtaattc ggtttcaggc ctcatttctt
     5101 taagatccgg attcagtaaa atatgtctaa ttgcttcggc tatttttgtg cctcccggtt
     5161 cgcgggttgt gatataagga atactttttt tctttagaac ttcttccagc atttggattt
     5221 gagtggtttt cccacttcct tctatgcctt caaaagtaat aaaaagtccg tgaggtaaag
     5281 agctttcacg gtgatagctt tcactgtgcg aagcactctt cttaaatgat tgtgtcatgt
     5341 ttatagtgtt catcatcgcg ttcaggatta tctaccaaaa aatgtgcgcc tcggctttct
     5401 tttctcatta aggcacttct aactacggaa atagcaatta tggccatatt acgagtttcc
     5461 actacttctt tacggacaga gttatgctgg tagaaattat ttatttcatt atagatattt
     5521 tccaggcggg aaagtgcata ttttaataat ctacggctgc gtctgattcc tgcataacct
     5581 tgcataatgg ttccaataat ttctcggtta tgacttatta ctacccattc attttcattg
     5641 aattcgccaa ttagttccca ttcagggata gatgggaacg aaacttcttc taaattggat
     5701 ggatgatttc ctgctttgta agccattacc aatgcttcca ataaagaatt ggaagctaaa
     5761 cggttagctc catgcaatcc acaacaggca acttctcctg caacaaaaag attgtggata
     5821 tcggtgatcc cttcaattgt agaaagtaca cctccacaaa aataatgagc tgctggagct
     5881 acaggaatag ggtcttttgt aaaatctata cctttagctt ttagcatatt attgatggat
     5941 gggaaatggc gtaacaaatg ttctgctggg acattagtag catctaaata gcaatactta
     6001 tctcctcgct ttttaagctc ggtatcaatt gctctggaga caatatccct aggagctaaa
     6061 tttccttggg gatgatattt ttccataaaa gtactaccat ccagaaggcg taaaactgct
     6121 ccctctcctc taagcgattc gcttattaaa aaggtttcgc catccggact ccaaaaagct
     6181 gtaggatgaa attgcacaaa ttccatattt gctaatcgtg ctcctgctaa acgagccata
     6241 gccattccat ctccagtagc aactttagga ttagtatccg gggcataaat ttgagctgcg
     6301 cctccagtag ctaacatagt ttttcgagat ttaataatat ctacgcggtt gtcttctatg
     6361 tctaaaatat aagcacccca acaagaaatt ccaggaataa aaccttcatc ttggacaatg
     6421 tgatgctgag tgatcagatc gacagctata caatattcat aaatttggat attgggattg
     6481 ctaatgcact gcgcaattag tgtttccata atttgatggc cggtagaatc agctgcgtag
     6541 gcaattctcc tacgggtatg acctccttcc atagtaagag ataaattttc caggcagata
     6601 tcgtaattcg gatctctccg cgtaaaatct gttcctaaat ctataagata ttgaattaat
     6661 ttaggccctg aagaaataat ttcagaaatc actttccgct tacccaatcc agctcctgcc
     6721 tgataagtat cctcgatatg agcttcaaag gaatccaaag tatctattgc agcagcgata
     6781 ccaccttggg catggtctgt attgcaatcg ttaatagagc ttttggttac tactgctact
     6841 tttccatatt tggaaacttg cagggcatat accaaaccgg cgattccact gccgataact
     6901 aaatagtcat attcataaat catagccatt atacaccatt actacagcat cctcttcagg
     6961 gattctatag tagtttttac ggattccaat ttcctggaat ccaacctttt tataaagatt
     7021 gcgtgctttg aaattggaga cacgcacttc caaaaataag agttttactc catcattatg
     7081 cattatttgc atcgtttctg taagtaagaa ttcacctaat cccattcctt gaaaatcgga
     7141 gcggacggca aaattgagga tacttttttc ctctttcaca ccataataaa agatatagcc
     7201 aataacttct tcatctttta ttaacacgaa agaccaggaa tttgtaagat tagcaaatgc
     7261 ttcatcagac cagggatcag agaatgcctc ttgctcgatt tttttgatag aatcaagatc
     7321 ttcgtctttt gctaatctta tgctatatga agcatccatt aaaacactct attatattta
     7381 catcttctcc ggagcactaa ttcccatcag ctcaaaacaa attcccataa ccttttttac
     7441 cgtaaacagt aacattaacc ttgaatgggt aagagcaggg cttttgggat ttactatttg
     7501 gtatctttca taataatgat gaagtaaaga agcaagctct tccaaatatg ttgttaaacg
     7561 atgaggctct cgatgaacag ctatcagaat taacagttca ggcaggtctg ccattttttg
     7621 gattaatgcc agttcttctg gtttggtcag tttattagcc atatcttttg taaagtgttt
     7681 aggaaacaac ttttcgctgc gagctttttt gattatattg cagatacggg catgagcata
     7741 ctgacaataa tacacagggt tttcgttatt tttctttagc gctaattcca aatcaaaatt
     7801 aagatgagca tttgccttac gagcaatgaa aaaatagcga gcggcatctt tacctacaac
     7861 acttatgagc tcatccatag taacgatttt tcctgcgcgt ttactcattt ttactctttc
     7921 gccgctttcg aaaatattaa cctgctgcag gaaaataaat tccaagatag aatcgtcata
     7981 attcaaagca cgaaaggcag ctctgattct gggaacataa ccatgatgat cagggccgaa
     8041 aatatcaatc aattttgtaa aaccgcgttg gattttagtt aaatgataag caacatctgg
     8101 aacaaaatat gtgatggatc catcagattt catcaatact ctatctttat catcgccata
     8161 ttttgtgctg caaaaccaga tagcatcttc tttttcgtaa gtacaatctg cttcagttag
     8221 ataggaaaga acttcttcaa ctactccttc tgagcgtaaa gttttttccg aaacccagcc
     8281 ttcaaaaacc acaccaaatt tttccaaaga atttcgttgc atttctaaaa gctcggagag
     8341 ggcaaattct ttcaagtgtt ccagacggtc tttttctggc atcataaaaa ctcgggtgcc
     8401 ttctaaagca ttcaaacggt gtgcgagatg ttttacatac tcgccatgat atgcttcgta
     8461 agggaattca cctatttttt ctccgtgaat ttctcgtaaa cgcaattcga gcgattctgc
     8521 taaaatatca acttggttac ctgcgtcgtt aatataaaac tctctggcag ggttataacc
     8581 tacatatttc ataatcctat aaatagtatc accaaaagct gcggcacggg cactaacaat
     8641 atttaagggg cctgttggat tggcacttac aaattccaaa agtacttttt ctccgttgcc
     8701 aaaatcgctt gatccgtatt cttgattttt gtatatctcc caaaaaacct gctgatataa
     8761 agaaggtgca agatgaaagt taataaaacc gggcttggca atttccacct ttttgtatgc
     8821 cttattcttt ttcagttctt taactatttt ttccgccagc tgcattggat tttgcttatt
     8881 ttctttgcct aaaactaaag cggcattggt agaataatcg ccaaattcag ggttatttgg
     8941 aatttccaca gtaaattcct tctcatggct tagttttagg gtatctaaag cctcaatgat
     9001 gttattacta agaagttgtt ttatcattta ttactctctt acgatgtttt atttacaaat
     9061 ttggtaagaa ggtcgtctat gtgataatac tctcttgttt caggtaaaaa aatatgcaca
     9121 atcaattctc caatatcaat caagacccaa tgtccatatt ccagaccttc tttactaagc
     9181 agctgcagat gatttttctt agccatctcc ataaggtaac tggctatagc tttattatga
     9241 atatcagcag tgccttcgca gacgactatt gtatctgtat aatcgctttt ttcttcaaca
     9301 tcatagatgc gtatattttc tgcttttttt tcttccagcc aggaaataac ggctgttaat
     9361 ttattgtttt ccactaatct cttcttcctt ttttgggtat aaattgctca taatctctgc
     9421 cgacgataat ttcaaaatca gcactataat attcattgta agcacttgtc catcgctgga
     9481 ttccggtcat ttttttcaat cgttccaaat cctgcatatc tcctttacga ataacgataa
     9541 tggttttatc ataaatcggc ttcggagttt gtgacaaact gactacttca atattcttat
     9601 ccttcagtgc tgaagcaaaa tccgcagcta aatgttcata accacaacca ttagtaacta
     9661 ctatcttaat tgatggaagc tggtcttctt cgtatccagt tttgggctca atagctttat
     9721 ttcttaatcc aaagaagagc acaactgctg ccgtagcaac aagaataagg ataatcggaa
     9781 accaaatttt ccttcttggg ggcaagttat tttcaggcgg gttatttgtc aaattcaacc
     9841 gttaatctct ttatttttta taaggttggc aaattcagga gccagtttat tataggcatt
     9901 ttcgaaagat tccggaatta ctctttcacc accaatcacg ctcataaaat tactatcacc
     9961 attccaacgc ggaacaatat gaatatgaat atgttctgca attccagcac cagcagcaca
    10021 gccaagatta ataccaatat ttattccgtc gcagttatac acgcgagtta agatctgttc
    10081 tgtatctttt aataaagaac acatttccag ccagatttta tcatccaaat cggttataga
    10141 tgcagaatgc tgatagggga taatcattag gtgaccattg ttatagggat agcgatttag
    10201 cattacatag caatttttag ctctatacac aatcaggttt tctttgtcat tatcaagatt
    10261 ttggcaacgg caaaaaatac actcatccgt tttctcccct aaaatataat ccaatcgcca
    10321 gggtgaataa aggtagtgtt tatccatatg attatcgttt tgattattca tactgtgtct
    10381 cttcaattat atgagagcca taatgtaaaa tgtggtgttc aggtaaaaca gcgttaacca
    10441 atacattata accttgcata atggcttctg tagaaacatc actattttcc agtaagcaac
    10501 agggaccaat aatgcatccc ttttccaaag tacattttcc tttgataact gtgttctgat
    10561 agatttctac atcaggttcg attatcacat catcacctat atagacgctg gcaggattat
    10621 gaatgataac tccgttattt agccattttt tacggatacg gcctatatag atatcttcca
    10681 aagcggccaa ttcttcctga gaatttactc ctgaaacttc catcagatct tccaaaacta
    10741 cattggagat tttttttcct gctctggata agatttcaac tgtgtcggtg agataatatt
    10801 ctccttgatg attattatta gaggtctgct ttaaggaagt aaataggtct tttgccttaa
    10861 aacagtaaat gccggtattc cattccttaa tttttctttg ttcatcggtg gcatctttaa
    10921 attcttttat tccacaaatt tcgccttcat tattgcgtaa aattcgtcca tattttccag
    10981 ggtcatctag aaaggcagtt aaaattgtgc aggatgcctg ttcttcttta tgcttttcat
    11041 aaagtcggat aagtgtatca gcagtaagta acggaacatc tccacataaa attagaacat
    11101 cctgatcata attagtaaac ataggttcgg taacgataac ggcatttcct gttcctaatt
    11161 gctcttgttg ttccacaaat tctatcctct cgttatcttc aagagcggct ataacattat
    11221 ttttcatata accaactaca ataaagacct tgttacactt cgtagctaaa gcagtattta
    11281 ccactctttg aatcattggc ttatctgcaa gtggcatagt cactttagca cgttcggatt
    11341 tcatccgagt cccttttcct gcagccagaa ttatcgctgc cagatcattc attatgttcc
    11401 tcccttgcta gtaattcata atataattca ttaatggttt tgtgtaaaac gggatggata
    11461 gcatcaggaa taagttcatt taataaacgt aagataaatt cccgattttg taaatccgga
    11521 tggggaataa taacctgggg ccagggtttt tttaatggag attttcggct atccaaaact
    11581 aaatcacctg ctaaaaggat atctatatct atattacgag ggccccattt aatccttcgc
    11641 aaacgtccca tagacatttc aatatataaa agattttcca ttaagtcctg cgggttatca
    11701 ttactttcaa tctcaagcac ctgattaaca aaatccggct gatttaacat tccataaggt
    11761 tttgttatta ttcggcttga tttacggaga atgcttattc cagattcggt agacaattga
    11821 tagatggctt tatccaattg actattgggg tcgtctaaat tggatccaag acaaagatga
    11881 tagatcataa ctatgcatta tccttttggt tatttctatc cctgcttagg attacttcca
    11941 cagattttaa attaccttga ataggtacag agggtttgtg aatacatatt gttagctttt
    12001 ccagtaaagg aaaatccgca agtaatttat ctgctatggt attagcacaa ttttccagca
    12061 aatagaactg ctggctttcc attatttcct ttatttctaa gtacactttg gtataatcta
    12121 tagtatcttc caacctccga atacctttat ctatagagga attggtatat aaggttacac
    12181 taacaataaa tctttggcct aaagtccgtt cttcttcctg gactccatga tatccataga
    12241 ataccatttc gttaagttta attttcacaa tttcctcgca acgcttattt tcaagcgttt
    12301 gccatatttt ttatcgatta accgtactat tctgaaggat aaacccatag caacaaaacc
    12361 tattaagata gacacaaatt cgatgaacca tagcgacgct atgataaaac ccaaaaataa
    12421 aaatattata ggtacaccaa aaactaacaa ggacaccaga gttctaccta caggagaaat
    12481 atccaattga actctatcgt tcacttgtaa gggaagatcg gttatgattt caaatttcgt
    12541 aggctcactt tctttgaaac aaaaaccacg cattgtacaa cttttacagc cgcctccagg
    12601 ttttatttct actgtaacac agttaccttc aacctttgta acaataccgc tatcttcaat
    12661 aatttctgca ttcattgttg tgtaccttgt ttaattcgct ctataatatt agtactgctt
    12721 aagccggcga taaaattcaa ggaataaact tttcctcctg ctttttgtac aatatcagcg
    12781 cctacgattt tttctatagg ccaatctcca cctttaacta acacattggg tttaatcaga
    12841 tctatcagtt cgtaaggtgt atcctgttca aagatacata cataatttac catttccaaa
    12901 gctgccaaaa caagggctcg ctcattttct ggaataatag gacgctcatt tccctttagg
    12961 cgttttacgg aggcatcact attaactcca acaatcagaa catctcccat cgctttggct
    13021 ttttctaagt atagaatatg tcccagatgt aaaatatcaa aacacccatt agtaaatata
    13081 atccgttttc cctgtttgtg caagttttca cttagttcgg caatttgatt gtaaggaata
    13141 actttagtat ggagcattaa agctatccaa gatctctttt gtagtaacac ttgcagtgcc
    13201 ttttttagct acagcaactg cagctgaatg attagcaatc aaggcagcgg attctatatc
    13261 ggaattaaaa agataagcta aagttaatgc actgataact gtatctcctg caccagaaac
    13321 atcataaact tcacgagcga aacttggtag atgttttact gctttattcc ctgtaaataa
    13381 gtacattcca tcactacctt tggttactac cagatattta acattcattt tttggcgtaa
    13441 ttcttctgca gacctctcaa aatcgtcttc atccgtaaat atccttccat aataactttc
    13501 cagttcacgg tagttaggct taaaaatttc aactccgcca tatgaactga aatttaagta
    13561 cttagggtct acagccacca gcttattatg tttcctggct aactgtagaa catcatttat
    13621 cagatcaaaa cttaacaaac ctttattata atcctcaatc aataatgcat cacatgatgg
    13681 cattattttg gtaagatatg cataaatttc tttctggata ttttcatcaa gctctgaaac
    13741 atcttctcta tctaaacgaa ctatttgttg acttacagag ccgatacggg tttttaaagt
    13801 agttggccgt gaagaatcca aaaaaatccc tgaagtggac atttccttat ctttcagctg
    13861 agtaattata gtagttgctt gagtatcttt tccacatacg ccaaccaaac ttacttgggc
    13921 accaagaact ttaagattta atgctgcatt agcagctccc cctaaacgat aatcggcaga
    13981 atttacttct attataggaa caggggcttc tggagaaatg cgttgaactt ttccccataa
    14041 atattcatcg agcataatat cgccaagtac aataatatga cgatgtttaa aactctccag
    14101 gatctcttca agtttttcag gtataattga gtgcatcagg aactcctata ggataattgc
    14161 cgtcgaaaca agcataacaa taatgttctg ggcagtggac acagctttgt aaatcacgca
    14221 atgataaatg taccagagta tccacaccgg ttatatcacg aatttcatca agggttcgtc
    14281 gattggctac caattcatgt tttgtgggtg tatcaattcc ataaaaacaa ctgtgcttaa
    14341 tttgagggct gccaatgcgt aaatgaattt ctgcagctcc tgcttcttta atcaaatcca
    14401 caattttgcg aatagtagtt cctcgaacaa tgctatcatc tattagcaca attcttttgc
    14461 cattaaagaa attcggtagg acattgaatt tttgacggac actttcatcg cgaaccgtct
    14521 gttcgggttt aataaatgtt ctgccaatat agtggttacg aattaaacct aaagataaag
    14581 gtattccact ttgattagca tagcctaaac cgataaaatt ggatgaatcg ggaaccggaa
    14641 ctaccagatc aggattcaat ccttcgtctt gtcttgctaa agctgcacct attttttcac
    14701 gaacttgata tacattttct ccaaaatgaa aactatcggg acgggagaag tatatctgtt
    14761 caaagataca atgtttttca catttattaa tgcgatagag attgggatca ttttccagaa
    14821 tctgtacacc cttttcattc actttaatta ttccggcagg gggtacttct tgtctatctg
    14881 taactcctaa agtatccaaa gcgcaacttt ctgaggctac aataattgtc ccttcgggtg
    14941 tttttcccca agtcatagga cgaatactat aaggatctct aaacatatat aattctgtgg
    15001 gtgtgcataa aacactggaa taggcaccca agatatcttt catcaaatgc ctgatagcgt
    15061 ctgtgatttc ttcctgataa tgcaataaac gataagcaat atattttacc agaagttcac
    15121 catcgttatc acttttaaaa tatactttct cagcttccag tgcttttcgc acttgagcat
    15181 aatttacaat atcaccattg gaagcaagag catagcaggg tcctgatagt gtttccacta
    15241 aatgcggttg tgtattaaat tccgttgcac ttcctttagt aggataacga acatgcccaa
    15301 tagctatatt tcctggtaaa gctgaaagct gctcattatg aaaaacctct tttaccagtc
    15361 ccatcttttt atgcaaacgg ataagaaatc catcaccgac tgccatacca caactttcct
    15421 gtcctctatg ctgttctgca aaaagaccta cagcagcaac acgggcagcg ttattatttc
    15481 cataaacgcc gattattcca cacattttag gttctaattt ccttttttgg taaatttaag
    15541 cttagattca gacccattta ttaaacgttc tagattgctt ttatgcttca ccacaattag
    15601 taaaacggct accgaaatca aaagtagggt tgcataatct gctttctctg cttttagtaa
    15661 tattgctaaa ctatgtgtat gaaaagccat agctgcacat attgaagcta aagaaacata
    15721 atggaaaagc acaacaacaa tcaaaaaaac aagaatagcc attgataaac tgataggtgc
    15781 tatggctaaa aaaacacctg ctgctgtagc tacacctttg ccacctttaa attttagata
    15841 gacaggaaac atatgtccca aaattacaaa aagtgcaatt atggatacaa aagagttatc
    15901 ctttcctaaa atagacatag ctaacagcgt tggaaaataa cccttggcta catccaaaag
    15961 gaggacaaac aagccggtaa cagttccaaa agctcttaaa gcattggttg ctccaatatt
    16021 tccactacct tctttacgga tatcttttct atgcaataac ttgcttacta tcaatccgtt
    16081 aggaatacta cctatcagat aagcagctat caaaaatatc cagggtagga atttgggata
    16141 attcatttaa tcctcttctt cttttttgcg ccccttaaaa ataagtttga tgctaactcc
    16201 ttcaaatttg aatgcttcac gcaattggtt atgaagaaat ctgcggtaat tttcagttat
    16261 caacgaaggg gtattgcaaa agaaaataaa agtaggtggt tttatttgtg cctgcgttat
    16321 ataaaatatt tttatatgtt ttccacttgg atgggtggga ggtctatgca taactacagt
    16381 ttccataaaa cgattcaatt cagaagtagg aatccgcttt tcgctttctt cttgaatttt
    16441 ggtcactgtt tccataatgc gattaatccg ctggctcgtt tttgcagaaa taaaaagcac
    16501 aggagcaaat tgcaagaacg gcatctgtgt atgcaagttt tgtaagtaca tatttactgt
    16561 atttgtttct ttttctacta agtcccattt attatacaca accagaatct ctttcatttt
    16621 gcgtttggca tagctggcaa ttttaatgtc ttgaacgcta agttcttcat cagcagcaag
    16681 aactaaaact actatatcag ctctgtctac agcttcaatt gtccttaagt tagaaaaata
    16741 ttccactcca taatttactc tggttttgcg tcggagtcct gctgtatcta ttaaaacata
    16801 atccttattg tgatagcgga aagggctatc tatagaatct cttgttgttc ctggaatatc
    16861 agttacaatt aatttcggat tacctaaaag tagattagcg atagaacttt tacctacatt
    16921 gggttttccc acaacggcaa tctttgtagc agaaggttta tcctcactct gataagcgtt
    16981 ttgagtgcca ggaatccgtt caatgagagc atctaaaaaa tcaccagtat tacgaccatg
    17041 ggaagcagaa agaggaaaag catcacctaa acccaactgc aaaaaatcat acagttccca
    17101 ttcatatttt tcgttatcag ctttattagc aacgagcata actttgtctc gatgtggata
    17161 aagaattttg gcaatatctc tatcaatatc tgtagcccca gtttgagcat ctaccataaa
    17221 tattatcagg tcgctttcct cgatggctaa aagtgcctgg tgtttaacca ttttgtctat
    17281 ggtttcagtg ctgtcaaaaa caattccacc agtatcaaca agtttaaatt ctttgccatt
    17341 ccactgcaca tcttcatatt tacgatcacg tgttacgcct gcttcaaaat ctacaatagc
    17401 aatgcgttta cgacacaaac gattgaacaa tgtagatttt cctacattgg gacggcctac
    17461 gatagaaaca atgtattttc tcatattatc tttaatttca gcaagaaagc agtattaata
    17521 taaacaggtt atacggttct ttcattaacc ttatggtcat taaaatatcc ggatatttga
    17581 gagcggatca gatttatcag ggcaaaacac ttatccgttt gttctgcttt tagataacta
    17641 atatcattgg caacgataag ctgagtttcc agttctctca atttcataaa agctatttcc
    17701 aaatattcaa tatctatatt atgatcttca atttgtacac tcatagctat attggaagga
    17761 acggcaattg aagtttgtaa catatcagtg gctaaactaa aagaatggct ttcgggcaat
    17821 tcactagtta atttgtaaac cattactgct aaatcaatac cattcttcca agcctcaagg
    17881 tctttatatg tttccatcat aatactcctt cttgctatca gaccctgtca ttttattcag
    17941 agccacacaa gtttatgtag tcaaattttt gaattagttg attttgtcaa cctccgttta
    18001 gtgaaatatg agtattttgt ccttaaagtt attcaaaaca ctttttttgg ggaagagcgt
    18061 tatcttttta ctctctgtaa agcgattcat ttcattatta ctacatctga aaaagctcgt
    18121 aaatttgtaa taataagatc cattctaagt tcttgggcat acataaattc attttctatg
    18181 cacttgttct gcttgacaaa aataatatct atttctatag tgaaataatg aaaaaactta
    18241 gttctgctaa ttacaaagat gtaaattatt gtctgcgttg cgggcataaa cttgagctaa
    18301 aaacagatag cgaaaataaa cttcgtcccc attgttctgc ttgcaattgg gtatattata
    18361 aaaatcccat tccggcagtg gcaatagtac tttttaacga aaataatgag cttttgttag
    18421 ttaagcgtcg aaatgaacct aaagcaggtt tttgggcttt gccaagtgga tatatggaaa
    18481 taaatctcac tcctgaagaa aatgctatac aagaattaga agaagaaaca ggtctaaaag
    18541 gaaagataat gcattgtgta ggttggtatt atggagatag tcctatctac gaaagaattc
    18601 ttagtattgg ttttcggatg caagtaattg gaggaaaact tcaagccgga gatgatgctg
    18661 aagaagcaaa attttatccc ttgtataatt tgcctgttat tgcctttgat gcacatcggg
    18721 attttatttc caaagagata gccacgcatc aagtaaacct tgaagcttgt agataagata
    18781 tctatctttt taatttttcg agactcaaga cctaaagacc ttcagatcca ttttaaggtg
    18841 gacaatctgc atttacagaa aaggataaat ttatgtacta ttatgtaata catttacctt
    18901 tagatatacc gaaagaattg gtttattgtt catccacaga aattgtggaa ggatccagag
    18961 tattagtaaa tctctcagga cgtttttgga caggtatatg tggaaggaaa acagttccag
    19021 aagagggcaa agatattaga tataaaatag ttacagaagt tttagatact agccctgttt
    19081 tatctccaga attgatgaaa cttggttatt gggtagctga atattatcat tgtgcagttg
    19141 ggaaagcact ttttgctatg ttaccttcgc gtttaattcc tgaaatggaa gcaactatta
    19201 cttggcttgc acaagaaatt ccagaatctt ttatttcact taaaagtgct ttggataaaa
    19261 gtgaaaatgc caattttaag aatttacgca agcttctacc ctcatatcct ttatataaaa
    19321 aaattgaaga aggagaagaa ctgggtttac tgaaaattga gcgtaagctg aatcataaag
    19381 ataaaccgcg cacagttaat tttattattc gattagaacc tattttagct gaagatattt
    19441 tacctttaaa acagcgcgag gcttgggaaa gaattaaaca ggaagagcta tcttttccaa
    19501 tggcaaatat cagttccact atttcttaca gtgccattaa ggcattagta aagaaaggct
    19561 ttatcaaaat tgaaccacga agatttgatc ctgaatctat ttctttcgaa agttcttctt
    19621 ctcccaaaca aatagagcta aatgaaggtc agcttgaagc tataaaagat attttacagg
    19681 gttttggaag ttttaatgta aatttactat atggaatcac cggtagcggg aaaacagagg
    19741 tctatattga acttattaga tattatttaa ataaaggtaa gactgttatt ttccttattc
    19801 cagaaattgc cctaacgcct caaatggtgg atcgttttca aagtagtttt gggcaaatat
    19861 tggctattca gcatagtcaa ttaacagaaa aggatcgcct aattcaatgg caaaaaatta
    19921 aaagtgggaa aaaaagaatt gtaatcggag cgcgtagtgc catttttgct cctttgccag
    19981 atttgggctt aattattgta gatgaagaac atgagagtac ttataaacag gatagtgctc
    20041 ctcgttatca gggaagggat ttatctgttg ttagaggtca aattcaaaat gcgcaagtta
    20101 ttttaggaag tgcaactcca gctttggaat cttggaataa tgccttatcc ggaaaatata
    20161 aattgcaaaa attggaaagc cgtccactgg attattcttt gccggaagtg caattagttg
    20221 atttaagaga tgaatatgat caaagcttaa tttcacctat tcttttagag gcaatagata
    20281 aacggttaca gaaaaaagag caagttattc tttttcaaaa tcgccggggc tattcatctt
    20341 ttatgcaatg cctaaaatgt ggaaaactaa ttacttgtac aaattgtgaa atcagtatga
    20401 gttatcatcg cgaccgcgaa gaaatgcaat gtcactattg cggaaacact attcccagtc
    20461 cccgaaaatg tccttcttgt ggatcttatt cttttgctta tggtgccccg ggaactcaaa
    20521 aaattgagca gaacctaaaa attctgtttc cggaggctaa aattttaaga atggattccg
    20581 attctgcccg gaaaaaagat acctataaat ctatgtataa tcgaatgaaa aaaagagaaa
    20641 tagatattct tttggggaca cagatgattt ctaaagggtt agattttcct ctggttactt
    20701 tggtgggcat tattatggct gatatatcct taaatcttcc tgattttaga gctgcagaaa
    20761 gaacatttca attgctaact caagttgcag gtagatcagg acgcagcgac aaagcggggg
    20821 aagttattat tcaaacttac aatcccgatc actatgccat cgtttcagcc agccagcaaa
    20881 attatatcaa ttttgctaca gaagaacttg gttatcgtaa aaaactatac tatccaccct
    20941 attaccgttt agccagaatt ctttttcaaa gtaccgatag cgaacttctg aaaaatgaaa
    21001 tggcagcgat gcaagagact atatatcagc ttcataaaca atatcctgtg ccacaattac
    21061 ttctgttggg cccttctgtt gctcccttca gtaaaataag ctcttattat cgttatcaca
    21121 taatctttaa agctgcttct gtaaaaattc tgcagagtgc tataaaaatg ttcaattctc
    21181 tttacacctg ccctcatcac ataaaaaaac agattgatat agatccaaat tctttaatgt
    21241 aaatttagtg ggcacttagg caatatcgaa aaaaaacaat tgctttgttt ataattgctt
    21301 ccctattagt atctcctcat cctctcctca tcaacctgtc ctcaactcgt tttcaactag
    21361 tcctcaatga tgggaggagt aggagaaaaa gagaataagt tgataagaag aaaatcaggt
    21421 tttgctaaat gtactctata tttcaataat aggtgaaggt ctattttgtt ctgccacaat
    21481 cagattaata tcgcttctga tgctttccaa atagtccttc attgtttttt cggctaaaga
    21541 gggaagattg tttatttcgc tcaggtgtgc taaaatcaag ttttttaaac ctggatgaat
    21601 aaggttagaa atcaggccaa ctgcttgaat attagaaaga tggccttgct gtccatgaat
    21661 gcgctttttc agattccaat cgtagggacc ttccataagc attctttcat cgtggttact
    21721 ttctaaaata agagtgctaa ctccgcttaa ttttagaact gacaactgag ttggatatcc
    21781 caaatcagtt gccacaccca gttttttatt tggttccgaa acagacgaaa acacaaaatt
    21841 acagctctcg gcagcatcgt gagaagaaat aaatgcttcc acgcaaatat caccaacttc
    21901 aaaaccggaa ccagtattaa aaataactat tctatcgttt atattaccca atctctgttc
    21961 acagcgagat agagttggtc ggtttatgta aataggtact ttcagtttac gggaaatgga
    22021 acccacactg cgaatgtgat ctgagtgttc atggcttact aatatcccgg taattttgga
    22081 tagatcgata ttatagaaat ttaaacactc caaaattcta tgcatactga ttcccgcatc
    22141 taaaagcaga gcggtatttt cggtttgaat taaaacgcaa ttacctttac tgccactggc
    22201 aataatagct gtttgaaaca taattcttag acaggcctat agattttata gtaacggttg
    22261 tttagtttgt cgtattctgt ccaaatggca tttttgggag ctgattcaat caattgagca
    22321 actccaactg tttgagcatc gagattatca caatggtgta aaacaactgc ttccaaggtt
    22381 tgaggcaaac gcacagaggc tttttcatat tctccgtggt gagatagaat taaatgtcgt
    22441 aaatttagca acagctcttc tggaaaacct gcaattttag aagctgtttc acaaactatt
    22501 tgatcggaaa gagttagatg cccgataagg cgtcctatat ctgtaaaatc aatattgctt
    22561 ttactgtcgt attcttttat ttttgccata tcgtgaagta atgctccgct gattagtaaa
    22621 tcataatcca caggatagag agtggaggca aattcacata aagcagccac agaaacagaa
    22681 tgttcaatta atccgtgtat ataattatgg tgccaacttt tcgctgctgg cgaagttaaa
    22741 aactgtgtaa aaaagtcctt atcatcaaag ataagatgta ataatctatt cagatatggc
    22801 tgctttactt tatcgacaaa ctcgaaaaag cgttcactga gaacttccgg agcaatttta
    22861 ctgcgcatca aatagtcctc tatattgtat tctgatctat cggcaaagcg caatttggaa
    22921 acagacaatt gagtttgacc tttataattt acgactgttc cttgaatatt gattatatct
    22981 ccagcatcaa attgttctga aagtttggct gcatctttcc atacattagc agagatgctt
    23041 ccacttctat cttgcaattt taaacgcaga tagaaatcat ttttgccttc gcgaagttct
    23101 ttttcagcaa ccagaaataa accggatatt tcctgattta ccttctgatg caattcggaa
    23161 atagttaaac gatctgtcat aacatccatc actttataat actaattttt ttaatttgtt
    23221 taatagcttt gccccgttca gaagtttcgc tttgcacaaa atatattccg gaagcgggaa
    23281 aaacctcaaa ggggacattt acttctgcat ttattaatcc cgttgtgaaa atcttctgtc
    23341 ctttcagatt ataaacggta aaaacagatg gggaagtgaa cccggatatt tttaaggact
    23401 gtccattatt aagaggattg ggatatattt ttaaatttgg acgaggtaaa ttatcatcag
    23461 aattagccac cgtccaccca taaggcaaag ttgcacattt agcataaaaa acttggcctc
    23521 cattagtgta agaattttgt agccaataat atacgcggta gctttttaaa gaatccagtt
    23581 cagaggtatt tattatcaaa tcaatttcca tactactatg cagattgatt gtgctgctta
    23641 ttggttcgtt ataaaccagc cattttttaa gagtataaag cgatctattc tcaaaattat
    23701 tttcctcaaa aattgcagcg aaaaatttgg gatagatagc agaagttgat gaccactcaa
    23761 atagagtagt gctgtcatta cttagggtaa tatttaaaag taagttttca ggattgtcag
    23821 gatctaaaat attaattctg gtttcactgc tggaaataaa acttcttttt tcttgtgcgg
    23881 aagaaatagc agttagaatt tgttcagaat cagcagcaag aggtagatga aatcttttat
    23941 aaccatctac aacagtttga ggtaaagaat tcatttggta gaaattaaat ctttgtgatg
    24001 cgcctaaaca agaaagataa tctgcctgat tgggaatgta aagtaaatga tgtaaacctg
    24061 aatcatctct gggaatatca tctgtttcaa aagtataacg cctaaaatat tcaattagat
    24121 gacagctgcc tgtttccata aagacattat aatatcgagc agggagaaca accgagggat
    24181 ggtttttaaa ggcaagattc acttttactt cagttggttc tcgattaaat gtgatacctt
    24241 cggaactaac aacccgtaaa gaatccctgg catttatatt ctgtaaaggg attagcagat
    24301 attgatccaa ttgtaacata ggatcagaaa aatgtacact aagagtatct gtgataggaa
    24361 aattactatg attataaaca gttacggaag gatatacggt ggaagaaggc aaaatgtttt
    24421 ctggaagtgt aaaattcaac aattccagat cagtattcag caaattaaaa tcaccaacgg
    24481 cacttatcct tatctcacag gcaaaacctg ctgaaaataa actttgccaa tattcattcc
    24541 catgaacagt agtattataa tagaaactgt tcttcccact cccttcggag gcagaaatgt
    24601 attttccggt tatgggagtg gttaattcca taaccaacca aacaacctgt aacggatttt
    24661 gaatttctgg aaacactata ttggttggat tagtgaagag ggttatattt tgccaatcgc
    24721 cacccggaat aatagaacca ggaaagtaaa ttaaatcatt gttctcggca tattgctcgt
    24781 tccaaaggga aactcgcatt tccatatttt gatagggatt ataaattttg aagctgttca
    24841 aagaaaaagt agcagaatca gctaatggat atgcagcttt aaaattgaag cgaacagccc
    24901 aaatagtagg accagaaaga tgatagtcat cagctaaatt atgatagcga aactggtata
    24961 ttttgctgtc ttcaatatct ttaataccac ttccaggaac cacttgaaga ggagatatag
    25021 cattactcat tataggtata agcattagga aaaaaagaat aatgagatga actagaaaat
    25081 tgataatttt ctgctttcct gaaatgtagt atcccgaaga agtgataaat aatttcattt
    25141 atcaagttcc gaaattatcg ttctggccat cgctatatcc ttttgaatag catcttttag
    25201 ttcatctacc tttttaaaca ttttttcttc tctaaaatag cggagtaatt caatttctaa
    25261 ggtttcgcca tagagatcgc cactgaaatc aagtatatgg gtctcaattt ctattattcc
    25321 actatttttc aatgttggac tgatgccaat atttgtcaac ccgaaatata ctttttcttg
    25381 ataacgaatc cgggataagt atatcccatt tttgggaatt agctgatgcg ggtaaagcaa
    25441 ttttaaatta gctgtgggaa aaccaatttt tctaccattg gaataactgt ggataactgt
    25501 accgatcaaa caatatgctc ttcccaatag attattggca gtttcaattt tgccaagaga
    25561 aagcaaattt cggatagtgc tgctgctgat aggtttacct tcataaagaa gtggttccaa
    25621 atattccact cgatatccta attcttggga atgccggcaa agaaattcat aatttccctg
    25681 acgcaaatga ccaaaatggg aatcataacc cataacaatt actttaggat gaaaagtggg
    25741 aataacatat tcttttaaga attttaaagg gggagttttg gctaattcag gagtaaaatc
    25801 caacagctca attctgtcta ttccaatctt tgccagctcc ctttgtttga gggttatcgg
    25861 acacagcatt ttgggtaaag ctttcgctct taaaatgaaa gcaggatgat ctttataact
    25921 gataaccaca ctttgtaatt tctctgcttg ggctatttct acaacttttt tcagcagctt
    25981 ctgatggcct aaatgcaagc catcaaaggt tccaatagtt aaaactgagt tagtattcat
    26041 tttcctctaa taggttgata tagcgttcaa ccctaattaa ctgatttata gttttatgaa
    26101 acgaattaag gaacttcata atttacctga aggtattcaa tagattgctg ttcctaaaat
    26161 tttgcaggtt tatagataat atagctgctt tgttcatttt agcacaataa aaggatgaat
    26221 aacattatta ctttttttcc ccaaagcaca aagctcatct tggtttagca gaagaattat
    26281 atcgctgctt gattctgaaa attctatatc cataccattg atcactttgt aaagctcttc
    26341 atcatttagc tttaaacaag tccaatccgg aaaaactcgt tcataactaa tctgagaatt
    26401 agtccaatta tctgttttca gatctcctaa agctacagca tcttgtaaag tgatctttcc
    26461 gatgctttct cgtttcagag atattgtgta agcaagagtt tgaatttgtt cagccagcca
    26521 ttcgcttagt gctctaatat atgttccttt gctaactttg catcgataag taagttcatc
    26581 gggggcattt tgttcctcaa ctaagaactt aaattcacta attttcacag gacgcatagg
    26641 aagttctaat atctttcctt ggcgagctaa ttcataggca cgtttacctt gaatttttac
    26701 agctgaataa gctgggacgg ataattcggc caaatctaat gcttttgctt ccaaagcggc
    26761 aagtttatca gcaaaaatag gaacaggagc tgtttgtata atttctcctt ccgtgtctcc
    26821 ggttgaggtt tttgctccca atttcattgt tgccagataa gtcttttcct ctttttccag
    26881 aaaacaggat aatcgagtgt aagaacctgt gcaacaaata agtaaacctg tcgcaaaagg
    26941 atctaaagtt ccagtatgcc caattttacg gattcctgta attcttctca gacgcctgat
    27001 aacttcaaaa gaggtaatac cagctggttt atttattgcc agaaaggcat taaccgttac
    27061 aggcatcgat aatgtccttt aacagcttgg cttttatctc ttctatatct ccatacatat
    27121 tacaaccgga tgcctgtttg tgtccccctc ctccataacg aacagcaatt ttatttacat
    27181 caatcgtttc tgagcgtaaa cttattttgt atacaccttc tttatcttcc cgtaaataaa
    27241 caattacctg aactccatta actccttgca cccaacgggt tacattcgtt atgctgtccg
    27301 gatcgatgtt gttttcttta ctcattaaat aagtggaatg catcattaaa atctggcgat
    27361 gatgatagag ttcaatggta gataaggtct ggcctatata acgcatttcc tctgcggatt
    27421 gatttaaata gaaattttta taaagggtat gaggttgaat tcctaaatca actaacttgg
    27481 cagcaaagtt aagtacttta gcatcagtat tagcattggt aaagttattg gtgtcattca
    27541 agattgtagt atataggcaa ttacacacaa atatacgatc agaagatggc attttattaa
    27601 tttcttcttc caggagagaa aaaataagct ctcctacaca agcaaaagaa ggatcaatat
    27661 aacgaaggtc tgcttttata aggccatttt cctgttgatg atggtccagt acaatagtgg
    27721 ttacagcatt atcaattaaa attcgtctgt cacctaaccg gtcataggaa ttacaatcta
    27781 aagcaattgc cagagaaaaa gtgcgctctt cttcatttag tgaactgaaa cgcttttcct
    27841 gaactttccc atcctgcaaa taatcataac ggctaagatc atcgttatct gttacaatca
    27901 cagaattttt ccctaaggaa gaaatgattc tttgtaaagc aagggctgaa caataaccat
    27961 ctccatccaa accaatatgc gaggtaataa caattctttc ggctttagag aattcactta
    28021 gtaatttaat tttgattttt tccatataac taacgacaaa aaaatcgcct tgaaaggcga
    28081 ttattccttc ttaattaatg taatattatt tagaaagttt caggcgttat tcttcgtcct
    28141 catcctcatc ttcgtcttcg aaatcatcat agtcgtcatc atcttcatca tcatcgaatt
    28201 cgaagaaatc atcatcatcg agataatcgt cgatatcaat gtcgtcatcg aaatcgtcat
    28261 catcatcgtt aaaattattt ttaactgaat caagaagaag atcaactttg gctgcgcgat
    28321 cctctgtctc atcatagtag aaggataatt caggtattgt tctcataatt tgtgctcctg
    28381 ctatttgttt tttaaaaaag ccagatgact tcatcaggag ttccctgatc tgttcatgac
    28441 tggcgggatt attgtaatga gaaaaatata acttagcata gtgcaaatct ttggaaatta
    28501 ctacatcagt aatttgcacc caagctagtt taggatcatt caacttctgc gaaagagcag
    28561 tgttgaagag ttttttcagc tcagattgca aacggggtat acgatagttt ttcattatag
    28621 ttttttacta atttcattaa tcacaaatgc ttccagaatg tcgccttctt ttatatcaga
    28681 gaaattggct aaggaaatgc cacattctga accagcacga acttcattta catcatcagc
    28741 atagtgtttt aatgaactaa tagtatcttc cgttactaaa acatcatttc gataaaggcg
    28801 aactttacaa tttgcttgaa ttataccctt atcaacttgg caacctgcta tggtgccaac
    28861 tttcttaatt ttgaagacct gtttaactaa tgcgctacca attacctgtt cttcaaattc
    28921 aggttgaagc ataccttcca aagcattatg aagatcctct attgcatcat agattacttg
    28981 ataaatcttg atctgcacac cttcatcttc ggcaaatttt cgtacttgac tggaagcgcg
    29041 aacattaaat ccgataataa tggcatcgga agctgctgct aaagatacat ctgcttcatt
    29101 tataccacct acacttttgt ggataatgtt tacgctaact tcctcattgg atagcttttg
    29161 aaaactatct gcaagagctt ctgaagatcc atctgtatcg gtctttaaaa tcacattcag
    29221 ggaattaatc ttttcttcct tgatgcgcgc aaatatgttt tgcagcgaac tcttatttaa
    29281 atatttttca cgttccaagc gaatttgttg acgctcacta ctaatactgc gggcagtttt
    29341 ttcactatct acctgattta atatatctcc ggctttggga gtatctgaaa gaccataaat
    29401 acgtgcaaca tcggaaggat aaataacttt tatctccgct ccgcgctcat tttccatttt
    29461 acgaattctt ccatgaacag ctccacaaac aacaatatcc cctttacgaa gggttccttc
    29521 ctgcattaaa atagtggcag aaggacccat tctggtatcc attcctgctt cgataactac
    29581 tgctgaggca ggaacttcac gcttggcttt cagttccata atttctgccg ttagaagaat
    29641 taattcaatt aaatttaata tcccttctcc ggttacaaca gaggttctac accagggaat
    29701 gtctccacca tactgttcaa gataaattcc catatctaaa agctgggaaa ttactttatc
    29761 tacattagct tccggaaggt caaccttatt gatagcaatt atgatagata caccagctgc
    29821 tttagcatga tcaatagctt ctcgagtttg agctttaaca ccttcagtag cggcaacaac
    29881 tattacagct atatctgtta cattcgcccc tcgagctctc atcgctgtaa atgcttcatg
    29941 acctggagta tcgataaaag taattttatg cttattaatt tctatttggt aagctccaat
    30001 gtgttgagta atccctcccg attcacctgc cacaatattt tcattgcgga tataatctaa
    30061 aatagaggtt ttcccgtgat ccacatgtcc cataatagta acaaccggag gacgcctttc
    30121 ttcttttact tcgctatact gttcaagttc gcggcctatt atatccattc cgtattcatc
    30181 ttcaaaacgg aaatctacct tgaattcatc acaaatcatt tccagggaat ccttgtccaa
    30241 ccgttgattc atcgtaacaa gttgacccat cataaaaaat ttggagataa tctcggaggg
    30301 ggcaacattc ataatcttgg caagttcgct tacagaagtg aattccctta tcacaatttc
    30361 attactacgc tcttcaacag tttgggcttc gcgatggtat ttttttcgtt tagaactctt
    30421 ctgcaaggtc tttttgatat tgcgagaaat ttcagcttcg tcaatttcaa ttacatcaac
    30481 aatcttttgc ttggcttttt tgctgctgga aatgatggct tttttgtgtt tacttttttc
    30541 tccaagctct tcgtggctta ctttcgcctt gccgaatttc cgttcttcta cttcttttat
    30601 aggttcagga acaggagcaa caacaaccgg tttagttcct ttctcagcag agggcttatg
    30661 tttatattgt tctgttttcc ctgcatcttt tttgtgcaga gttgatggct taggagcaac
    30721 cacttccggt ttggaaggtt ttgaatattt tggaggagat acaggttttt ccttttgggg
    30781 agctggcttt acagtccttt cctcagcttt ttcttgaacc ggtttagcaa ctttcttttc
    30841 aggttcagca atgggtttta cttcctctgg ttcaggaaca actgtaattt gctcaaccac
    30901 aggagcaata atttcaggtt ctttttttgc ttctggttct ggggtttctt gctttgcttt
    30961 agctgcttgc cgcatttcta ttaatcgttt gcgatctctt tcagctcttt tttcagcatc
    31021 aacctgttcc ttatatttaa tgcgtatttt atctgcaact tcttcatcta ttaagctcat
    31081 atggctttta acattcacac ccaagtccgt gaggtgcttt tttagtgcca tcgtgcttat
    31141 tttcagctct ttagctaaat cataaactct tattgtcaac ttatcctcct ctctgtttca
    31201 gagagccaat actccataat tttgccggct atatgttttt ccttaatacc aaatacaccg
    31261 gttatagaca atcccaaagc agaacttatt tcttcctggg ttcctgtttc aataacctca
    31321 agggattttt tcgctgcacc agcaatatgt ttcatcttat ttttagttcg ctctgaatta
    31381 tcctgagcca ggatcaacag aaatacttct tttctgccaa tagctctttc acaagcatcg
    31441 gtaccaacaa ccaattttcc tgcttttcgg gcaaactgca tcaaagttag gatcttagaa
    31501 ttcatcttat acttcctaaa ctttttttct gataccgttt ttgccacttt gaaagacctt
    31561 taaaacactc ttcatttggg cacaaataat acttgcgcac ctgaattatc ctgttgcaat
    31621 caaaaacaat tccacttgtt gtgtgaaaaa aatttagcag tcgattctgg tcaacctttt
    31681 tcttgcacac tacacaagtg cggataggaa tgtgtttagc tttactattt ttattcgaca
    31741 tcagaaatat tttgctgcct cttttaggcg ttctgctgtt ttttgcccca ttccttcaag
    31801 gtttcgaagc tcttctatag aagccatata aacatcttga acactggtat aaccggcttg
    31861 tcttaaaacc tcagctatct ttggagtaac tccatcaagt tctgctatat gacttatagt
    31921 tctacgttct tttgccattt tttcttcata ttcaggcatc gtatatatat ctattttcaa
    31981 acctaccagt ttggcagcaa gttttacatt tttgccctgt ttacctattg ccatcacttt
    32041 gtccgattct tcaactatta cactggcaga atgattacgc tctataatta ccctttttat
    32101 acctgttaca cctaaagcat tttcaaccat cttttctgga tcgtcactat gaataacaat
    32161 atctatttgc tcaccatgca attccttacg gatcgaatca atgcgagttc cttttggtcc
    32221 aacacaagca atcacaggat cgatttttgg attagcagtt tccaattcaa ctttggttcg
    32281 aattccaggc tcacgaacga ttttacggat tttgatttcg ccggtgaaga tttcaggtat
    32341 ctccgcttca aacaactttt taacaaattc aggattggtg cgggaaagaa ctataacaac
    32401 tccatctttt ccagaacgaa tattaactac ataggctttg atgttttctc ccacacggta
    32461 aaactcattt tcaatctgtt catccaaagg tagtaacgca tctgtgtaac ctatatcaat
    32521 aatgtagccg ccaaaatcat cgatggcttt gatctttcca gttacaattg tatgcttctg
    32581 tttattaaaa tcattctgaa ttttgtcatc ttcaagctta cggatcttat cctgaattat
    32641 cttctgaata gttttgatca gcttgggctc aaactcataa agagacatcg ttttttctat
    32701 gaattgcccg agttgaactt caggatcata ttcttcgcgt gcttcggtta gagaaatctg
    32761 gccaagacct tcttctcgtt ccactactaa actttggaat tttaccttga tattaccact
    32821 aagatcatct atgtaaactt ccagctcaga ttcaggttcc aatcgctttt gcaatgttga
    32881 tataacagat tccaatataa tattctgaat catttccttg tccagctgtt ttatggcagc
    32941 aagtttacta agggcttcaa gcatatttgc actcatttct taacctctca tcttaggaat
    33001 ggctaaaaac acagttttag cacgggttat agacttaaac ggaatttcaa ttatcgtgcc
    33061 tctgtcatca agaacaatcg tgtcctgatt tacttctatc agtgtcccca taacagattt
    33121 cttttcttct ccttcactat attgaactgc cactttttca gaaatggcac tcacataatg
    33181 actcttcaac ttcaatggac gctctaatcc aggagaagaa acctctaaaa aataacgttc
    33241 aggaatgagg tcaaaggtct ccagttcctt acctaaggca cgactaaaac tggcacattc
    33301 atctaatgta acgcccccaa tcttagtaag atagacagtt attacccgtc ccttgccagt
    33361 catcttttca tctatgtcat acaaagcaag atgcatttcc tggcaaatct tcttggctat
    33421 ttcttcaatc tgagttctgt aaaactccat tctttttttt cctcattcct tttttattga
    33481 tgaacaacat cctgtattgc ttcaggtaac tgagttattc ttttcttata gattatgtta
    33541 ataatttgct tagccttatt agggtttagc gttgccaact ttactttgca tattcctata
    33601 aacatatcaa cctttaacta attattatta aacatctata tcttttcacc tcaaaaaaaa
    33661 tagctatcca atatagctat ctatttccgt agttaacggt ttccgcttct tttgcaccga
    33721 tttatttatc ggattctata cataaatcct ttatcaaaat atgggtatta actgtcaagt
    33781 aaaatcttcg aaattaggtt aaagctttgt agtgaaagtt gataaatagt aaattagtaa
    33841 tttattattt taggtatgag ggatatattt tgtacatctg gaattaccac tctcatcatt
    33901 attttacatt tactcaaaat gttcatagaa tggtataatt ctgaaggtac aaatagcgcc
    33961 ttacttaatc ttccaactaa aaaaagtcct ataatcctga ttgttcagct ccaattcttt
    34021 tatcagccgt atatgtatta gtaaatttct gtaacagagc tctctgcttt tttattataa
    34081 acctgttttt ttgagctttg aaaagaaaga gcaggtctat gtatacattt cttttaccct
    34141 caataccgta gagtaaagaa ttaattgaaa gcagaaaata caacaagtta ttcaattatt
    34201 cgaagaatgt aacggttttt gttggtttta gtctcaattt ggataggtga aaaaaccagt
    34261 tatagttaaa taaataatta cttaaggtcc taacaaaggt tattatttat atctcttcgg
    34321 catcctgacc gtagatttct ttgaagcgga aattatctat ttcggcagga atgccttcga
    34381 agaccttttt accttctttc aaggcaagaa ctctgtttcc atatcgtctt gccagggtta
    34441 aaaaatgtaa agaacagata atggtgattc catctttgtt tatttctcct aaatattgca
    34501 taatggaatc tgcagtggca ggatctaaac tggcaactgg ttcatcggca agaattaaag
    34561 agggattttg catcagggca cgggctatac caactctttg ttgttgtcct ccggagagga
    34621 atttcacttt tttattggca taattttgca gacccacttt ttccaagttt tgcatagcaa
    34681 gctcgtaatc ttctttacta aaagttgaga gagaagaatg atagccaagg cgtccagtta
    34741 aaacattagt aagcactgta aggtttttaa ccaagttaaa ctgctgaaac accattccaa
    34801 tttgtcttct gtaatgccta agttcaattc ctttaagggg taggatattt tgttcccgga
    34861 agaggatatg tccactgctt ggttctatca agcggtttat acaacgcagc aaagttgatt
    34921 ttccgcttcc tgaaagtccc agcagaataa taaaatcacc ctcctgtact tgtaaggata
    34981 gattgtttaa ggcaagaata tgtccatcgt agcttttgtt taggtttata acttccagca
    35041 gcttgttttt agaattcatt tcaataaatc agcagggtcc atattcatag attttaaggt
    35101 agtgcgaact atttcataat cctcatctgt agcatattcc aagccgtcta tatcataaag
    35161 tgttttcaaa acttttttcc cttcaaggct ttgggcatag cgatataagg tctgaacaat
    35221 caaaccttgt agttcgggag gaaggtcttt acgaaaagtt acagtatcat ttgggatcca
    35281 atctgtataa ccgacaattt taactttttc aaacacatca ggataggtgt ctaagagttt
    35341 ttctctagca tccataggtt ttccagctga atctgacggt gaccaataac tacatcctac
    35401 atcagctctt ccactataaa cagctaaaat agcttgagga tgacctcccg cgaaaaaata
    35461 atctttagga gtgatcccat tttgtttaag tatagcagag ggatagatgt aaccggaagt
    35521 tgaagctgca tctgtatagg caattacctt gccttgaata tcctgcaagg attttatatc
    35581 actatcgctg cgggctataa attgaccccg gtatttgtgt aaaccgtttc gcactgtcat
    35641 caattgcaca ttggcatcat acttttgttt tgccaaaata taggcataag taggaagcca
    35701 ggcaatgtct gcctgatagg ttcccagggc ttcaataact gcagcatagc ttgttggaac
    35761 agcaacttta aaatgtaatt tagtttcgtt ttctaaatat ttggcgatag cttctccact
    35821 ggaaaccact tttcctgctt ccaaagaagg gacaaaaaac attttaatgg ggttttgttt
    35881 ggaacccagt tttcggttag ctttttctaa acggctttct tgattgccac aacctaatat
    35941 tagtagggct ataaaggtaa gcaatatata tcttttaagc ataaacacca tctcctttaa
    36001 tctcatgtaa aaaactatct ttttatctgc aggatgaggt caagtaaaaa ttataaatag
    36061 tcccattctt caaagaactg gtctattaat aaaataggat tattccaggt ttcttgaaaa
    36121 gtaatgcggt cctttccatc cagggtttca ccttcggtat taaaaatgca ggagggtaga
    36181 ataagggttt cattcttttc aggagaaagc tgagaataca aatcggaata ggtaatcaat
    36241 ccgcaaacat taatatcggg gccaaaaaag tcattgttaa ttacttggac gcgaatccgt
    36301 tgattctcca gacgtttatt gaggtcttct gcaattttgg atataacttc tttagcgcat
    36361 acagaactaa tcattaagta gtctactggt ttttttcgta gttccttcag gattgaacgt
    36421 tttttcagtt tataattttg tatggttaac cgcagcatac cgattccgtt ttccaactga
    36481 gggtaatctg catagtaatc ttcatcaggt atatttcggc cggctaaaac aaaaaactca
    36541 tcggcaggat aaataatatc actgctataa agatttctgg cttcttcgat caaatctaaa
    36601 acttctatag cgttatgctg attgaaggtt ttcaaatcac aaaggttttg tctatatttc
    36661 gttaaaccaa caggaacgat tccaatcgag aggcagttta gttttgtttg atgcaggtct
    36721 tttaaggttt gttttaatgc ttctccatcg ttatagcccg gaacgcacac aatttgaata
    36781 tgaaagctga ttccatattg gcttaaattt tttaaagcgg atagagcatc cacttgtcgc
    36841 agaggacgca tcattttttg cctcaaagta ctatcggttg tatgcaagga aatatataag
    36901 ggagtaattc tttgcgttat tatccgctca taatcttcat tagtaagatt ggtaagtgtt
    36961 atgtagttac caaacacata ggaataaaga tagtcatcat ctttcagata cagggtattt
    37021 ctaaggtgag gaggcatctg gtcgataaag caaaatatgc aggaattttc acaactgcgt
    37081 atattatatg gctccagttc aattcccaga aagctctttt cctctcgctg aattttcact
    37141 atccgctttt cacctggggg agtcattatc tccagttcaa attcatagtc cgatgtgtat
    37201 aattccaaat ccaaaaaatc gcgtatttcc tgtccattaa ccgtaagcag tatttcgctg
    37261 cttttgattc catttttagc tgccaaactt ttaggaagaa tgttttgaat tacaacggac
    37321 atagtttttc aggattatct tttaagtgtt ttttactcta tcgtaacgga atttaaaacg
    37381 cttgattcct tcaaagattg tgcgagccaa gcgttcttga tattcttcat tcaccaataa
    37441 ttgttcttct tcagcgttgc tgataaaacc aagttcaacc aaaatggatg gcatatacgc
    37501 acctcgtaac acatagaaat ttgcctgtct aactcctcta tctatacctt gggtacccat
    37561 aaccaaattt tgatgcacgc tggtagccat attattgcta tgttccagat tttcggtttg
    37621 tagaagatcg cttaatataa aatccagatc gtcatatttc ttctttgctt cgctgccgcc
    37681 ttcaaagcgt tctacaactg cattttctaa tgcttctaca gctctggcat cagaagtttg
    37741 agcggtcgaa agataataag tttcaattcc tttagaactt gttgacttac tggagtttga
    37801 atgtatactt atgaaaaggt ctgccttttt ttcattagca aaacgagttc tatcataaag
    37861 agaaacaaaa cggtcatctt ctcttgttag taaaacagta attcccaatt ccttttccag
    37921 cagtactttt agtttttggg caacggcaag attgatatct ttttcatttg ctcttaattt
    37981 tctacctact gcaccaggat ctttccctcc atgacctggg tctagaacaa tagtttttac
    38041 gctgttatca attggttttg atatttgcag cgtgtttcct tttagcttta cttgtgcagg
    38101 aaaatgagta tgcagatgtt catttacgaa agtggaaggt aaatataagc gttcaccttt
    38161 tcgctgtaag ggataattca tattatagga tactgctttc agagtataat aggatgacct
    38221 ttccagaaag ataaactgct catcatagat gtttaaataa agccgattgt ccgttctatc
    38281 ctgtttacaa acggctttga aaacactgca aaattcctcc aattctacat aagggactga
    38341 agcaagagtt gtttccttca aaatgtgggg ttttgtttca cctttaatct ggatatttat
    38401 attaccgaaa agcatcccta ggcagctaat cagcattaag attgtaatta ttttttgttt
    38461 catatagttt gctcctttat agggttaaat tctgaaccac catatacatt atttggttca
    38521 ggtgaaaaat ctggtgtcca gagttcagaa ccggaaattt cctgagtata tatatttgta
    38581 aaaccaagag caacagctgt ttctatcaca tcttgatatt cttttggaga tattcctctg
    38641 cgtaattctt tataatcgtt ggccttacct gcaggatagt attggcccat gatggagagg
    38701 gttaaatcta ccccaatttc attcgctaat gtgtataagt tttctttgca acctgctaag
    38761 ccatagggca aaaccagcat ccgaataata ataccttttt tggcgattcc attactatca
    38821 acttctaaaa atcctacttg tgagaacatt tctttaatag cggcaatagc aatagcagga
    38881 taatccgatg cagaagaata ttttcgtgca tataccttat gtgcatattt gaaatcgggt
    38941 aaatatatat ctaccagccc gttcagagac tgtaaagttt ccactttttc ataggcggag
    39001 gaattccaga ctatgggaat agctaatccc tcttttttag cataaataat tgcttctcgt
    39061 agttgacgag taaagtgagt aggagttacc aaatttatat tatgtgcacc ttctgtttgt
    39121 agtttgaaca tcagattaat aagttcatct ttagttatat cttttcccca gcaattagat
    39181 gagatatcat aattttggca aaaaacacag cgtaaattac atcccgaaaa gaaaatagtt
    39241 ccactacctc tggttcctgt taaaggaggt tcttctccaa aatgccgagt aattaaatca
    39301 acttttagag tatctgtaac tccgcaatag cctatatgct tagttctatc gattccacaa
    39361 ttacgcgggc agatattaca ggaagacatt gatgaataag gcatttaatt ccttttaagt
    39421 ataaaagctt caggcagaag ctctttgata tttattatct gcacagtatt acggttggca
    39481 tatatcacat ttatatcagg ggcaaattca taaataacct gacgacaagc tccgcaagga
    39541 ggaaagcttt cttcgctatc cacaaaaacg gctatctcag taaaatcttt atgcccttcg
    39601 ctaattgctt taaaaatggc attacgttca gcacatatag ttaaagaata tgaagcattt
    39661 tccacattac aaccagtgat tatctgctca tcaacagttc ttacagcaca accgacttta
    39721 tagttggaat agggagaata agcattggca gatgcttttt tggcttcttc cagcaattct
    39781 ttttcccttg gtttcagttt tagcatttta tatcctctaa gctaaaagag ccaaaattaa
    39841 cccaccagca ataactgaac caatctgtcc tcccacattt acacttaccg ctggcatcaa
    39901 taaaaagtta aaaggatctt ctttcaaacc catctgatga ataacgcgag cactcattgg
    39961 aaaagcggaa attccgcaag ctccaatcat cggatttatc ttttcctttt tgaatatatt
    40021 catcagtttg gcaaatagca caccacctgc tgtgtcaaaa ataaaggcaa ccaatcccaa
    40081 tgctaaaatt aacagagttt ggggaaccag gaaaagttca ccttgcattt tggacgcaat
    40141 tgttatccct aaaaggatag taactaaatt acccagctca ttttgagcgg ttttgctgag
    40201 accttctaat acaccggatt cacgaattaa attaccgaac attaaaaaac cgattaacgc
    40261 caacgaggca ggcacaaaaa tgccggctat aattgttatc gcgattggaa atattatttt
    40321 cactgtttga ggtacatcac cactatgata atccattctt atacaacgct ctttcttagt
    40381 tgttagcaaa cgaataaccg gtggctgaat aattggcacc aatgccatat aagaataagc
    40441 tgctacagaa attgctccta acagattggg agcaaaacga ttggcaacat atatagaagt
    40501 gggtccatcc gcagctccaa taattccaat actggctgct tccttaatat caaaacccaa
    40561 ggaagctgct aacacgatgg ttacaaaaat gcctaattgt gctgcagctc caaataatag
    40621 cataaaagga ttttttagca aggggctgaa atcgatcatt gctccaatag caataaaaat
    40681 taatagcgga aacaactccg tatcaattcc agctttgaat aaaatcccta agggagaatg
    40741 ttcaccaaca gctgcagaaa gaggtatatt agctaatatt gtaccaaaac caatgggcaa
    40801 caataaagtc ggttcgtatt tatgcttaat cgctagccag attaaaacca gacctattag
    40861 aatcattata acctgtttca attgaaatcc aagtagtccg gtaaacagtg tttccatcca
    40921 caaatctcct aattattaac tatttgccac tattgtgaaa agggtttgtt tgtcaagcaa
    40981 atgtagctgg agaaaaatac gaatatggaa aagtgaaact tgattttatc agtaaaagat
    41041 ctccttggtt ataaggttta gggcattgat atggtaagga ttacacctta gttaggaatc
    41101 atcactacct aaaatatccg ttaacattct tacggtttgt ttggcagtgc acattcataa
    41161 cggagctaca aactctatag ataagataaa gacaaaagtc aggaaacata tatgcccaaa
    41221 tgttctttac taagtaacag atatttaagc ataatggctc ctgacttttc ttttgaagaa
    41281 ggtatcttgt tttcccttct acaccatttt ataatatatt ctatctcata ggatgttcat
    41341 tccaggtttc acgcctttcc ataaggttca ttatttcatt agaatcattt ttttggtgga
    41401 gctatatttt cctgatttca tgcggtaaaa atataatccg ctggaaacgc tgttgccgtt
    41461 atcatcttgt ccattccaca caaatttatg gtttccggat tccagatttc cgctgaacaa
    41521 ggtttttact ttttgtcctt tctggttata aatatagagt tcagtaagat tagggctact
    41581 gagattgaaa ctaattgtag tttctggatt gaaaggattt gggtatgctt catagagaac
    41641 tgtttccaaa gctggaagtt gtggatctac aacaggtgtt ggttgatacg cattacaagt
    41701 tctaccaaaa ccacccaaat aattaggcca cagtaatgcc tgggcgcctc ttttaacatc
    41761 gataatggaa agtccggaat ttgtagggaa agcgatttca agatcgttgt cgccgtcaat
    41821 atcagccagg gttggagata cttttaaaat tccacctatc tcaataggaa aattgggtga
    41881 ttctgttcca tcaatacgaa ccgaatgtaa tttacctgaa gaatcaccaa aaattattcc
    41941 ggcattgttt ccaccatcaa atctggcaat tacaggagta cattccacag cggtattaat
    42001 tccaataggt gtattgggaa gatctacacc cataccatcc ataatataga tatttccgtt
    42061 attggcaatt aggacaattt cttttatatt atcattgttt acatcaacca cgacgggact
    42121 ggttttaatt tgcgttccga tatttttttg gaaaagcaca ctgccattat gattaacacc
    42181 atataggtat ccaccggagc ttgaagcaac taaaatttcc ggttcaggat cagaatccaa
    42241 gttagctata caaatcgcat tttgagatcc acctcctgta agagaaacag gaaaacctgg
    42301 tagggattga ccattttgcg agctaatggc attcagagat ccatttaagg caatcataac
    42361 aatatccata taaccatcgt tattaagatc tccaatagct ggaggagcta aatatgcacc
    42421 agttacagtt acaggaaaat tagtaaagtt actaccatct ccattaaaaa tattcaaaat
    42481 cccgttttga gtaacacagg caacttttaa ttcggaagct gcattgggac ggaaaataac
    42541 aggaccattt cgtaaggttc cccctgatgc attattccat tttacttcgc ctaagtcatt
    42601 aaaacaaata acctgattat tttgtaagca ggcaacaaac tctctattag aatcgttatt
    42661 gatatttccc atagctatag aaccaataat aacagaagat gtctgaacag ggaaaccggg
    42721 ataactggtt aatccatctt tgcgtaaaac ttcaatgtta ccatcggctc caacgaaaac
    42781 aacttctttc tgaccatcat catccatatc tattaaaaga ggggtggaat ttgttgctgc
    42841 accagaataa aaaggccatc ctgcttgatt taaagatagt gatatatcta taggaagggc
    42901 tttattatag gggtattgat tatcttgatt agcctgcaca taaaactgga agggtattgg
    42961 ttctgaaggt aaagtagaaa ctgtctgaaa cttgaaaggc tgattgttat tcagtaaagt
    43021 tgttccagca tgcatagtcc caaaggtagc tacagaatca ataatggtta ctccaggata
    43081 gtggcaacgc atagtaactg ttacattttc tgcctgtaac caaccaagac ctgtgatagg
    43141 atcaatgcaa ttgcttaggg caattctcaa tttaataact tcgccaggat tggctactcc
    43201 atcatcatca ccagaaattt cttccaggat ataatcctct atttgcatat tggggatttt
    43261 atcatacata gttgcagtat aagcatttat cctgccagca cccaacaaac caacataatc
    43321 tggatttaag ctatcaatat tatcagcagt agccatcaag cgttgcatca attccacggg
    43381 tgttaaattt ggatttagag atttaactaa agcagcaaca ccagcagcaa ctggactagc
    43441 catagaagtt ccatcataag ctgcatatcc atctccagta ataattgtag aaagaattgc
    43501 tgctccagga gcgcatatat ctattgtaga tccataatct gaaaatgaag cttttacatc
    43561 tccttgagca acagcagcaa cacataaagc gtttgtacaa tcagcaggat aatcttgata
    43621 ggaagcatta tgttctgtgt tttcattacc ggcagcggta acaactaaag cccctaaggc
    43681 tgttacatag ttaactatgg aatttggata agttccactt cccacactac cccaactggc
    43741 atttattatg tcagctccaa tttctccagc atatttaact tgatcgtaag cataggaaac
    43801 tccagttgat ggagatgtat tagaagatcc tttacaacaa agaattgaaa catttggaca
    43861 agttcccaca acgccaattt cattatcacc aacggctcca gcacaacctg ctacatgggt
    43921 tccatgatca ttagccgcga aattttgcat tggattatta tcgttattat aaaaatccca
    43981 tcctacaagg tcatctactt tatttccacc ttctcctgca tcaacaccat ttccaccact
    44041 aattgtgccc gcattccaat caattgtcat accaggagat tcagcaggat ttatccagat
    44101 attagctctc aaatcaggat gattccattt tactccagaa tctgtaatag ccactaaaac
    44161 atcatgacta ccaagcgtat aatcccatgc ttcaaaacta tgcactaaag tatgagcata
    44221 ctgtaaatct aacatagggt cattagtaac atacttagga cggttgatac cttcaaattc
    44281 tgcataaacc aaattcgggt ccttagataa aagttccact gctttatcca tattatcatc
    44341 actttctaaa tagagacggt agtgattttg caggtaaatt ccattatcgt tccattcagg
    44401 aacttttaca taaggatgca tctgtttcat atccacaata tgcaattcct ttgccaactg
    44461 gtcaaatgaa ctcatccccg ttgaaacaat tccatcttgc acatcaaaat taacaactcc
    44521 atctaaattg ggaatggctg ttttcgtaaa acaagcaata attgttttcg aatagaatag
    44581 ttcgggatca aaattgattg ccgtaagaac gccaatcgct atgatcagca ttaaaagtat
    44641 actcagcttt ttcatttcta cctcaaaggt atttttttcc ctctattata taaagggaaa
    44701 tttagtcaaa tcctttttta tttatttgag acactaatat agaataagtt cctctttctt
    44761 agtatattga tgatggtaca ttttttttac gatagcattt tttatctgcg aaaaatcagg
    44821 atcagaccta actacttcaa aggcatcctg acgagctatt tttagtattt cttgatcgcg
    44881 cacaatatta gcaaaacgga attgaggcat tcctgattgc tcaaaaccaa aaagatcacc
    44941 cggacctcgc aattccaaat ctttttctgc tataataaaa ccatcggtag ttttagtcat
    45001 agtggtaagc ctttgccagg caacatcgtt aacaggttga tgttcaatta aataacaata
    45061 tgcttgttca ctacctcttc ccactctacc ccgcaattga tgtaattgag ctaaaccaaa
    45121 atgttcagaa tgttctataa tcattacatt ggcattagga acatctactc caacttcaat
    45181 aactgtagta gaaaccagta tttttatttc tcccgcttta aaacgcttca taatagcgtc
    45241 cttttctttc acaggcatct ttccgtataa taaacaggca gggtattctg gaaatatctt
    45301 tttggagatg tattcataaa gcttggtagc gtccagaaga gatatttttt ctgattcttc
    45361 tactaaaggg caaacaatat aaacctgcct ccctttttcc agctctttgc gcacttcaat
    45421 ataaactgta tcaattttac tagaaggacg gataattgtt ctaactggtt ttcgcgtggg
    45481 gggaagttca tctaaaatac tcacttccaa atcaccataa acggtcatag ctaaactgcg
    45541 cggaatagga gttgcagata aatataataa atcaggatgt aatgcctggc gagctaaacg
    45601 cgctctttgt tcaaccccaa aacgatgttg ttcatcaaca caggcaaatc ctaagcgttt
    45661 aaaacttata tctttctgta tcagggcatg tgttccaatc acaatttgag cttcaccttc
    45721 ggcaattgca ttttttgttt cctctttacc tttatatact ccacccttaa gtaagcagac
    45781 cttaatctca gaatctttca aaagttggga gattgtttca taatgttgtt cagcaagaat
    45841 ttctgtggga gccatcatag ctgcctgata atcattttct acagccaaaa gcattgcaaa
    45901 aagagatacc actgtttttc cactacctac atcaccttga agcaaacgac tcatctgttt
    45961 ttcggaacac atatcattaa atatttccca taacactttt ttctgtgcac tagttagcgt
    46021 aaagggcagt tttctataga gagcagttgt gagctgtttt ttattgataa aattaattcc
    46081 tgtggttgtt gtagtatgaa acagtttatg tctcgcccat aaaagctggg aatagaaaaa
    46141 atcctcatag gcaaaacggc gccggactat ttcaatctct tgcggatttt gcccaaaatg
    46201 catcttttga agggcaatat gtctttccag gaaatgatgt tttgtcaata tatattccgg
    46261 caaattttcc tctattaaac cagtataaag agagaaagca ttatacataa gctgacgcat
    46321 taacttttga gaaatacctt ctgttaaagg ataaacaggt aaaacttctc gctttttcca
    46381 aaaatcatct ttcgtatctt cgctatcatc aatcaattca aaatctggat gcaccatctg
    46441 cagctggcca ttaaattcag aaattgttcc atttaaccaa actaatctgc ccgggataaa
    46501 caatttttca tatacgggag ggtaagtaaa ccaggaacat ataagcccaa cttttccatc
    46561 actgacacct atattgagta tctttttgcc tttcaaagaa gtatgaacat caacccaaga
    46621 aatcatagct gtaaaagcaa gcatatcacc tggtttcaaa tcttctaaat gagcacttaa
    46681 tttccggcta atatataact taggaaaatg ttccattaag tccaaaatgg tcttaatacc
    46741 cagcttacct aaaagtcggg ctcgattttc gccaacccct ttcaggtatt taatttcgct
    46801 atgtaaatct gtagtttttt tgctttctgc cataacaaaa tccaatatag ataataagct
    46861 ggatttgtca atctccgcat taacaaagtt aaattatgtg aagaactata cctaaagtgg
    46921 gagatgatac caattttatt attgtcatat ccttatttgc agcttggcaa tctacataaa
    46981 agtgattgta tcttactcta tattaagcta aaatataagc tataaaaaac cagtccttct
    47041 taattatctg cggtggaatt gccgtagggc tgctcgggtt tttgcccatc agcaccaagg
    47101 taacagcagg gtagctatca aggaagaaac aagagtgttt atctaaaaaa aatagtattt
    47161 tggttaatgg ggaagttatc tgttttacaa ataaaaagag gtagatctgt ctgtttctat
    47221 ttcgggataa agtacgcatt tagccaaatc taaagattga caacaaaaca agtataatat
    47281 atattgaacc aatatatgag gatattatga agagaactgt tctaatggca ttaatggttg
    47341 gttttgtaat ggcatccagt cttcaaggag aaaatctgtc tgcttcactg caaacaaata
    47401 ttgaaacaga aaacatagcc tacgaagatt attctcctct tactgaaaaa gatatttatg
    47461 aactaaaaaa tctgttgcaa gaaattcaat taaacccttc agatctcaat tttgagaagg
    47521 attgggacct ttctacctgg ggtaagagcg aagaattttt gcacgccttg caggaacctt
    47581 attatggttt gaaaaaaatg caggaatata gaaatgcagc aaataagtca gatacggatg
    47641 atggctttgc ttttttggca gatttactat ataaaaatgc tatcgatgat tcgggctcga
    47701 tttctattta tcgagaatat aaacaaatct atcagcagaa tttttggcgt gaagttcagc
    47761 agccccaaga cctttttcct ttttatgaaa ctgctctgaa ggatattatc cctattttgg
    47821 ctgaggctta tgcgaattat aattctgagc tgtggagtaa atatatttcc tatctattta
    47881 cggctctgat agaaagtgaa gataaagaag cttactctac ctattttaat cagcataata
    47941 tacctgaaac aagagatgtt gaagattgcg agatcaagaa acaaatgggg cttcttttgg
    48001 agccagctat cttgaaagca ggtatgcaat ttctggcttt aggggatgtg atgaagaatg
    48061 aagcaggaaa actactctat aaaaataaga aacccataat taaaaccggt aaatttggaa
    48121 caatgattat cggaacggtt cacgatgatg tttatagtga aaaactatta aaaaagccac
    48181 ttattctgct tattgatccc tcaggaaatg ataaatatga attttctctg gctaccggtt
    48241 atgataacta tacctatctt ctgattgatt ataaaggaga tgatagctat ttgaacccaa
    48301 aaatagggga gatgtttttt gctttatcgg gcttgggaat ttcctatgat ctacagggaa
    48361 atgatattta taaaacaggt gatttttctt ttgctgcaat gatggggatg caattacacc
    48421 gagattttca aggtaacgat atatatcaaa gtggtctatt tagtcaaggt gctgcaattc
    48481 gagggctttc gctccttcaa gattgccagg gaaatgatat ttataacgct acttgccttg
    48541 ctcaaggttt gggttcaatt cgttctgcag gtgctatatt ggatatggaa ggagccgata
    48601 attatctggt tgggggaaaa tattttcatt cacctttgat gcctaatgac tatttaaccc
    48661 ttggacaggg agttggtttt ggtttgcgtc ctgattttgc tggcggattt ggccttttat
    48721 atgataaaaa tggaaatgat cgttatttgg gtggggttta tgcccaagga gttgcctatt
    48781 ggtatgcttt ggggatgttg attgatgaag ctggaaatga tgtttataat gcaatttatt
    48841 atcctcaagg ttcgggaatt catctggcgg caggtttcct ttttgatggg caaggagatg
    48901 atgcatatta cagtagacac ggtcctggac agggtgcggg tcacgattgg ggtttaggaa
    48961 ttttaattga tgcccagggt aatgatgcct attctattga aggaggaaat ggtttaggtt
    49021 taagtaattc cgttggaatt tttgtggata aaagcggaaa tgatcgctat gaacgcaaaa
    49081 attcccaaaa ttatggttat gctgcctata gtagagctac aggtagtata ggtctttttt
    49141 tggatgaagg tggtatagat acatatccgg atacccttat ggcaaatgga acaatatgga
    49201 aaaagggaac ttatgctgtt ggaaaagaca taacccaaac agaagaaaaa gatgatctta
    49261 caaaaaatga aatgcctatg ctaactcctc ctgctcctga tgattctata gaaaatattt
    49321 ttgctgctgc tgctgaatgg gaagttggta atgccattga aagagtaaaa aaagccagag
    49381 aaattatgct tgacagagct gaagaggcaa gcgaatatat tcttaaatat aaacttaata
    49441 caaaatccgg attggaatat cgcgccttgg aagttatggt taaagaaaac gagagtttta
    49501 aacagctact atttaactat actgacgatg cggatagttt gaaagctaaa aacgctttgg
    49561 cattaatagc aggtgttgga gatagcactt taattgaacc tataagaaaa cacctgaata
    49621 atggaaagta tattactacc tgtctttctc ttttaggcag tatcaaaagt gaagagagcg
    49681 ttaatttact tgctgaatat gcatttcatc cctgtgaacg ctatcgctat ttggtagcgc
    49741 gttctttgaa acaaattaat actcctcaag ctcataacta cctgataaag atggctgatg
    49801 acagttcttt tctggtaaaa accctaattc gaaaatttaa agaggaacaa caatgaaaga
    49861 cgaaaaacaa atctttgagg attatcttaa ctcacttgaa ctggaaaaca taaaccaatt
    49921 aatgaaacgc tttgaacatt atcataattt gctgtatact actaatcgta aggttaatct
    49981 ggtttcccgt aatatacctt cggatagata ttgggtgcag cattttcttg acagcttagt
    50041 tgctttggaa tgtttggatt tagaaggaaa atttgttttg gatttcggtt cgggaggtgg
    50101 tttacctgga attcctctta aacttgccgt tccagatttt aatttaacac ttctggaatc
    50161 ggtacaaaag aaatcccggg ttttgcgtga atttgtggat gccctatctc tttcagcaac
    50221 aaatgttgaa gcgagtcgtt tagaagatta tgcttatgtg gcaaggcgtc caagctacga
    50281 tataatagtt tgcagagcca tttctttgga ggaacgttat atttctccca taaggagatt
    50341 gctgaaacca agcggaatgg ttgtttttta taaagctcag aaactggatg atgtgcaaaa
    50401 cctgcattat gaagtcctgt tggataaaaa atatccttcc ttgggtcaac gcaaattaat
    50461 tggtattaaa cagcgagacc ttatgctgcg ttaagtttat aaaatgaaag gatatatatg
    50521 cccaatatta taactgtagt aaaccaaaaa ggtggcgttg gaaaaacaac aacagccgta
    50581 aatctggctg ctgcattggc agttttagaa aaaaggactc tattagtgga ttttgaccct
    50641 cagggaaatg ctaccagcgg agttggaatc gataaagata aggt
//
LOCUS       DAOSTH010000009        41888 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_1043_length_41888_cov_13.526929, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000009 DAOSTH010000000
VERSION     DAOSTH010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 41888)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41888)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41888
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1043_length_41888_cov_13.526929"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(507..1649)
                     /locus_tag="PLB65_02780"
     CDS             complement(507..1649)
                     /locus_tag="PLB65_02780"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018455 - alcohol dehydrogenase [NAD(P)+]
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOQ77482.1"
                     /translation="MEAFSFCNPTRIEFGTGKIKILGMELHKAGIKSCLMVAGGGSIK
                     ANGVYLQVCESLNKSGIRFVESWGVQSNPTLQKVNELCELAKEEKVEAILAVGGGSVI
                     DTAKTVAAGVYLQNIWNAFLGKERIRNALPIYTVLTISATGSEMNGNAVITNNETLQK
                     WSIYSPLIYPHLSIIDPSVQSSLPFKQTANGAMDAIAHILEFYFADDRAIATLAIDDA
                     LQKTIIEMTDRLQLNSGDLLARGNLAWCATLALNGISGIGMKGGDWACHSIEHSFSAL
                     HPDIAHGEGLGVIFPAWIEYMSEKDPSRFLRWAKNVWDEENVSRALHRFRDKLESWGM
                     AQTLRDLGIKENELPQIVNNIMSSPRIGMVSKLSAVEVESLLMLAY"
     gene            complement(1905..1980)
                     /locus_tag="PLB65_02785"
     tRNA            complement(1905..1980)
                     /locus_tag="PLB65_02785"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1945..1947),aa:Ala,seq:cgc)
     gene            complement(2161..3159)
                     /gene="gap"
                     /locus_tag="PLB65_02790"
     CDS             complement(2161..3159)
                     /gene="gap"
                     /locus_tag="PLB65_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011897301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOQ77483.1"
                     /translation="MTNVAINGFGRIGRLVLRIIINSHPELNVVAINDLTDAKTLAHL
                     FKYDSVHKVYPGQISSEENYLLVNGKKIRIFAEKDPEVLPWKDLGVEYVIESTGIFTT
                     KEKASKHLKAGAQKVILTAPAKDDVDATIVMGVNHTSLKAEHLIVSNASCTTNCLAPV
                     AKVLQDRFGIVNGLMTTIHSYTNDQRILDLPHNDLRRARAAAMSMIPTSTGAAKAIGL
                     VIPELKGKLDGVAIRVPTPDGSLVDLCANLEVEVTKEDINSAMNEAANSYLKGYLMYT
                     EEPIVSIDIVGNPHSSIFDSLITYTKGKMVKVFSWYDNEYGYSNRVVDLLEYMRKL"
     gene            complement(3176..4150)
                     /locus_tag="PLB65_02795"
     CDS             complement(3176..4150)
                     /locus_tag="PLB65_02795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOQ77484.1"
                     /translation="MFLSGLQMKEVFLKAKKMRFGIIASNVVFDTQIRALIQGYAAVN
                     SDGLLQMSTGACKYAAGSSQDIQIGARMISAMAKTFASQFPKCGIGLHIDHATPNYFD
                     FIKFCIENDLVTSVMIDASKENLEDNIRITKEVVDLAHKYNILVEGEIGHIKGAEDEI
                     VSEDELYTKPEEALEFVKRTNVDLFAASVGTNHGVCKGENIVLRLDLIKEIDDLLIKN
                     GVERGLVLHGASGLTDQQQKTAIANGVVKINKDTHYQMDMASAIQEYWEKEKYAIVCP
                     PDMDPNLYVPNKSKFDPRKWLIKGEEKMQNTVMDFCNISGSAQNSILL"
     gene            complement(4332..5105)
                     /locus_tag="PLB65_02800"
     CDS             complement(4332..5105)
                     /locus_tag="PLB65_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HOQ77485.1"
                     /translation="MAMNKADFSRTELLIGEKGLQALSKTCIAVIGLGGVGSYSAEAL
                     ARSGIGKLILVDFDIVEPSNVNRQILALQSTIGKYKADLMQQRILDINPQAEIVVHRE
                     VLNKDNQEQLLAEADYCVDAIDSLNSKIDLLEFLVRNKKRFISVMGAGNRLDPNLIHI
                     STLDKSHNCPLARRVRKLLKQREIEINFPCVYSSELPIKPNQKSNNPSIEHPNISPKI
                     VYNNSQRIPIGSISYIPAIMGMMASSWVIKSILKDSTDN"
     gene            complement(5071..5814)
                     /locus_tag="PLB65_02805"
     CDS             complement(5071..5814)
                     /locus_tag="PLB65_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOQ77486.1"
                     /translation="MNLIDAHCHLANLNQLMPVKPLLEEAEKLGIFFWLSSVLTQSEI
                     AWHQNNPDNRIAYSAGIHPCYDECDLELEDIEILCQKKRIWAIGEIGLDRNNPNYECQ
                     VELFIAQLELAKQYNLPVVLHLVGHTTEAYKILKQYNLQYLVHGYAGSLQGYKELLRL
                     NTLFTISERLLKKDKKELLLAMIDSKRYLFETDITREYVKVGEKNPLLRLSSLVSSVA
                     DLSGIMLEQLITEQQRNFNGYEQSGFFQN"
     gene            complement(5801..6841)
                     /gene="waaF"
                     /locus_tag="PLB65_02810"
     CDS             complement(5801..6841)
                     /gene="waaF"
                     /locus_tag="PLB65_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HOQ77487.1"
                     /translation="MKEPERIIIVQTAFIGDVILITPLIRAVAKLFNKTKIDVLVVNE
                     AAKLLENNPYVNEVIIDSKRKNVWQSTVQLIRYIKNRHYNIAITPHSSFRTHLILYLS
                     KIPERIGFDRGFAKWMLTKKVEHPVGPHKIIKNLGLVKPLSNKEFDIQTELFPSANDK
                     HKAEELLKALSGKTLIAIAPGSIWKTKCWELNSYISLCRKLLDSGYGIVLIGGESDKP
                     QCDEIEIAIEKNNANLINLAGVTNLLESAAIIEKCSLMICNDSGAMHIANAMQTRVFA
                     FFGPTVPLFGYYPYREGDKVFEVDLKCRPCGSHGAKKCPLDHHNCMKLIAVEPVLEAV
                     NLLFLRRNNEFN"
     gene            complement(6838..7920)
                     /locus_tag="PLB65_02815"
     CDS             complement(6838..7920)
                     /locus_tag="PLB65_02815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOQ77488.1"
                     /translation="MRVLHIDSEKTWRGGQQQAIYLYEGMLKRGFDCGFLCLPKSSLH
                     NYLAKNNLPFHTISCKGELDLYAGFKLAMLCRKKSYDVLHLHSSHSLSWGLMAKLFCP
                     HLKLVATRRVDFPIGKNPFSAFKYRTKAINKIVTVSNNIRNVLVDCGVPESKITVIHS
                     GIDIKKFADKKVPENFRETWKIPEKSIVVGTVAAFAGHKDYPNFLQAASLAVKENPEL
                     FFMAVGDGILLPEMKKVAMEFGLQGHISFTGFQKEIGHFLKAFDIFVLASYLEGLGTS
                     VLEAMSVGLPVIGTKAGGISEMISNEENGLLVPPKNPQELSHSILKLASEPRLRQKYG
                     KNALETVKQFDKELMVDKNLELYKTL"
     gene            complement(7917..8663)
                     /locus_tag="PLB65_02820"
     CDS             complement(7917..8663)
                     /locus_tag="PLB65_02820"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ77489.1"
                     /translation="MDKISAVLIVKNEANNIARCLKSLSGVDEIVVLDTGSTDATENI
                     CVGFGAKVYHLDKWEGFGKAKQKAVDLASFDWIISIDADEEISPELQKELQTLKHNSF
                     ENSAWRIKRRSYYLGKPIHFCGWQNDAPVRLFNRKKGSFNTKIVHEGIQTSQPIKKCK
                     HYLYHYTYPTREVHFTKMRFYAELSAKQLYEKGKKSNEFSAWIRGICKFFKMYIFQLG
                     FLDGAKGFLLCKNSAWGIWYRYHLLGKMQK"
     gene            complement(8664..9815)
                     /locus_tag="PLB65_02825"
     CDS             complement(8664..9815)
                     /locus_tag="PLB65_02825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOQ77490.1"
                     /translation="MNNLERLLGLPFFYTGTLTDKILKWNNSSSLFFLFPHYHTGGAE
                     QIHIDILRIFASHRPYIIITNKSRNKVHYKEMQECGKLIDMSKYLNNANRFIVKYFFL
                     GFLSSLINRHHKSIVMSSNSAFFYRLVCHLNDSYLIDIIHGLFGINHHNWLQATPKID
                     RRIVVSNSILGRIQDIYRKNGVDSIYDCRLQLIYNSVPLCDLIPEKNYYAPLQIVFIA
                     RNAQEKRFYLYRKLAQNVIREHPDIKFYCIGNFVETEPVINLGEIVSRKMLYEIIQNF
                     HILVLCSVSEGFGLVIVEGMTCGLIPLATDVGGVKETFQDRINGMLVKAKDEQKIIDE
                     FTSYIKELNVNRDLLQKMSCSAHEYAKEHFSYDRFAAEYLRIVVEGKNS"
     gene            complement(9812..10648)
                     /locus_tag="PLB65_02830"
     CDS             complement(9812..10648)
                     /locus_tag="PLB65_02830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ77491.1"
                     /translation="MNLSVIIPVYNYLDLLEKCLFSVYRQAVQPDEIILSDDGSEEDI
                     IGFFQVQKEKTSIPMILVRQEHQQFRAARVRNNGVSVCNSEILAFLDQDIIIPKNYLL
                     SIKNELTIGRFLSGYPVRLSKDQTEKLTFEMIEQNNWNAILTEAQKKKIIKQYRKDYL
                     SYVLCKYLPFVEHGAKMRGGVSAIFRNDFEAVNGYDENYIGWGNEDDDLGKRLHALGR
                     IGFNFVLSQLPLHQYHQPYHNKGERKNSGYSKSRRNEIFPNNYRCQKGLNSKRNDLYI
                     LK"
     gene            complement(10645..11589)
                     /locus_tag="PLB65_02835"
     CDS             complement(10645..11589)
                     /locus_tag="PLB65_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77492.1"
                     /translation="MKISGFSFVRNGIKLYYPVIESIQSILPICDEFIIAVGKGDADD
                     DTKERILALNNPKIKIIDTIWDEKLCKRGMINSWQTDIAKKECTGDWLFYLQADEVVH
                     EKYLPVIQKRCEELLNDKEVEGLLFAYKHFWGDYYHYHNGHGWYPYEIRIIRNNPNIH
                     SYQSAQSFRYFEYYDNPRQETGTRKLKVAKVDAEIYHYGWVRPPNLMQNKCKALNSVH
                     WGKEKAEEYYNKAPKYFDYGPLSQLAFFEGTHPIVMQNMITNFNWQDKLQLTGKPNPY
                     RELHKHEEFKYRFLTFIEKHFNGGKQIGTFKNYVLLKR"
     gene            complement(11624..13540)
                     /locus_tag="PLB65_02840"
     CDS             complement(11624..13540)
                     /locus_tag="PLB65_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ77493.1"
                     /translation="MAKQRKNQKRWNNIILIFRMMLRYWHYLVAGFICMLFFTLFNGV
                     SVTLAIPLFDYVFKPGRNNLLYTDFSGFLTALGELIKQHFGSSGGFFAAIQNYNSLWE
                     GCKKLMLQTDSLSLLYGLCVAIFVIILLKNGFYLLHKIFFNSLRGKTIRDIRNYMFTR
                     YLNQSLTFFGRHRVGDAIVRMVGDVDIVSEQLINSFVNVTREIFTVLVFARIAYLLNP
                     TLLLYSIIVLPVFSFSIGLLGKKIKKYSKRIQEQISNLFSNVEEVLNSMKVVQAFRRE
                     DYEKKIFESINQKHYRMWYKAQYYGALNTPIAELNTAITGILVIIIGGNMILAPGSNF
                     SLGDFTAFLFALFSMLHPLKVITQVYTDIRKALVSLDRIGLVLNQESRIQDKPDAIVK
                     DSFDREISFDNVTFGYKNTKPVLKNFNLTISKGSKVAFVGSSGGGKTTIANLMNRLYD
                     VNSGAITIDGIDIRDIKLDSLRKLFGVVTQESILFSRSIKENIAYGSQDEVTDEQIIK
                     AAQIAYADEFIDELPNKYEEVLSSKGSDLSGGQKQRLCIARAIVGDPPILIFDEATSA
                     LDTDSEQKVQLAIDKVTLNRTVILIAHRLSTILKADQIVVLEKGKIVGIGTHQELLES
                     CSRYQHLYNLQNGK"
     gene            complement(13586..13662)
                     /locus_tag="PLB65_02845"
     tRNA            complement(13586..13662)
                     /locus_tag="PLB65_02845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13626..13628),aa:Arg,seq:acg)
     gene            complement(13969..14247)
                     /locus_tag="PLB65_02850"
     CDS             complement(13969..14247)
                     /locus_tag="PLB65_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77494.1"
                     /translation="MGMKFKTMIPTFIPIVMEMMRVFKRNSKHDQDIKKKDRTAEKLG
                     SLEHLMVRLEKQVQHNRELYTKSLNKIRIWLCLNSILLIAILIKLFLG"
     gene            complement(14258..15103)
                     /locus_tag="PLB65_02855"
     CDS             complement(14258..15103)
                     /locus_tag="PLB65_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77495.1"
                     /translation="MKKLLFACLLLIPATMLLAVYDDYQPSAIARGLGGAYTAVSRDA
                     NAIFYNPAGLVNTTLSAKLGFSQLYNQDFSELKTVALGYKLPQKLGTVALGIRAMDVD
                     YEDVNLMSEQVYSLAHSFYLLQDIHSQISFGYTGNFYLLSMDGEDDDTALGLDLGAMA
                     VLHTRTRLAFAVTNINKAKMGDENQIDLPSKLALGISYIPYDKVTTSIEVKKDFAKET
                     EFMGGVEAHLFEPLAIRFGVHQNPATYNAGLSIYIRDLELDYSYTYHSVLPGTHYFSL
                     GYNFK"
     gene            complement(15112..16545)
                     /locus_tag="PLB65_02860"
     CDS             complement(15112..16545)
                     /locus_tag="PLB65_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77496.1"
                     /translation="MKNYLLTFFALVFITGISAQAVLTCYDIQYTTDPTGNSPYYNQT
                     VTVQGIVTHIIPNSAFYIADPEGGEWSGLYVYHRNTSNVVSVGDLVKLTGTVDEYFNL
                     TEFTNVSAYEIISQGNALPPFLELSTANMPSGTNHPENEKYEGVLVRFQDVTIKSSPD
                     GYGQFNIADSSNIWAMVDNGLYAIPANSIVIGESWYIIQGIVDFHSSAGFKLNPRNAN
                     DMIKQDTIENSIIKIVRTDPTTDPLINTDVSMNLISTKIKAEWGVQSYSVSFKLNPAC
                     IHFSGFDTEGTLTPSIPDYYISSAGDTISYTVTYQDGLIYNQNDALLIKILVKPLVYG
                     ESVIELVSFKYNDTNVLNLNDGSLVTKLQKKTAYLDISKIGDKKNIFNPEMGEQIIIS
                     YGTKTGYLSKAIVRIYDAQGRLVYTPVHTNITSSTGIENFIWNGRDANLKLLPPGLYY
                     CHLEVIDRSSGDKDNTVQPIVIKSALK"
     gene            complement(16546..17112)
                     /gene="lspA"
                     /locus_tag="PLB65_02865"
     CDS             complement(16546..17112)
                     /gene="lspA"
                     /locus_tag="PLB65_02865"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOQ77497.1"
                     /translation="MKPPLTKLKTAPAYFLMMLIVVMDQLTKTLVRVNMKLYEHIPLL
                     EGLFGDTFLLIKVENPGAAFSIGLGNDLVNRIFFISITVIVVGLIIYLLRRAQHQIQV
                     IAFGLILGGAIGNLIDRVLFGPVTDFFSMDFPDFIMERFPVFNIADSAIFIGVCLLII
                     DMLFIKDNSSDSVEEDEQLIINESDKEL"
     gene            complement(17109..17510)
                     /locus_tag="PLB65_02870"
     CDS             complement(17109..17510)
                     /locus_tag="PLB65_02870"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HOQ77498.1"
                     /translation="MAAKKLSPDKLRHFEELIRGELRESLDYINNINKEQSLGARESS
                     GDLSSYAYHQADQGSDTNLMEQTVMMLESEREKIRQLNEALRRIADETYGVCEICGEF
                     IQETRLEIIPYATCCVACQEKSEDKKRRQKR"
     gene            complement(17573..18403)
                     /locus_tag="PLB65_02875"
     CDS             complement(17573..18403)
                     /locus_tag="PLB65_02875"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HOQ77499.1"
                     /translation="MNNYQETAIWLKNKFSEKPLIAVILGTGLSDLPEAFPVLCSLPY
                     NQIPGFVKTTSPSHPGNLLLCEVKGIPIIFLQGRFHYYEGYSMQEVVFPVRVLASLGI
                     KVLIVTNASGSLRKELKPGAIVQLQDHINFMGTNPLIGINDDNLGERFPSLNNPYDPE
                     LCSLADEVAKELEISTFKGTYIAVTGPSLETKAECNAFAILGADLVGMSTVPEVITAR
                     HSGIKVLAYSIVTNYSNLFHNLAHSQEEIQKTALNSGRQLQKIIAEFIFRVTKTFSLT
                     "
     gene            complement(18404..18988)
                     /locus_tag="PLB65_02880"
     CDS             complement(18404..18988)
                     /locus_tag="PLB65_02880"
                     /inference="COORDINATES: protein motif:HMM:NF016959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HOQ77500.1"
                     /translation="MPLFPLDIRHQEFSSSMFGYSKKEVRAFLDQVASDVETYQKQLQ
                     KELARQEQIQNEVKQEAVQAGSAVEELKRREELISRTLVFAEKTKADIIANARKEAEN
                     IIKEADLKAKRAIQEAKQYLSVLEHQYLQLKEQKRQFLIQFKVELQSFQDRISKDPLL
                     SKDSELMLDSEFHQIKDDFVPKPENNEPQNTKSQ"
     gene            complement(19272..19835)
                     /locus_tag="PLB65_02885"
     CDS             complement(19272..19835)
                     /locus_tag="PLB65_02885"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HOQ77501.1"
                     /translation="MLNIVNQKTGWIEVICGSMFSGKTEELIRRIRRAEYAKQKVQVF
                     KPIIDNRYDANNIVSHSQMQAASIPINSPGEIYSYLDDDVKIIAIDEAQFFDSSIVGI
                     CNDLADRGYRVIVAGLDQDYKGEPFGSMPQLMAIAEYITKNLAICVICGNPANRTQRT
                     VHQDEQILVGSTDAYEARCRNCHEVLD"
     gene            complement(19900..20637)
                     /locus_tag="PLB65_02890"
     CDS             complement(19900..20637)
                     /locus_tag="PLB65_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOQ77502.1"
                     /translation="MINNIAYPSFSAEHLRYLSKLKQKKYRLQEKLVIIEGKRTLEQL
                     FSWGIKAIELYVTNEEPTITAEKIYTISEQGLLRICDSEHPQKIAGLFPLPVARKIAF
                     RKAFYLDGISDPGNLGTIFRIAAAFNIDCLILSPNTCEVSSPKVIRASLGAVYKVPFF
                     NCAAENLKELNANIFALDMQGKIPLANFEQPSEPYIVALGNETKGLSSSILEIATETL
                     CIPMLGEMESLNVSISAAILAYTISEK"
     gene            complement(20813..22489)
                     /locus_tag="PLB65_02895"
     CDS             complement(20813..22489)
                     /locus_tag="PLB65_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HOQ77503.1"
                     /translation="MKYVLIILLLSIGLVLNAESWTVLVYMAADNNLAEMGRLDINSM
                     ESVPQPEGLNLIVQADFPEGAKRFRIQQDNSEDISSPVLANLGIIDSANPEVLNSFIN
                     WGFNNFPSQRKMLIIWSHGDSWYKGNDPKWICPDESSQNLISVAEGELTLALTDIPKL
                     DVLLFDACSMQSIEVINEIGFCANYIIGSEDLVPQYGFPYEKIIPLLEGDLDILIAQI
                     PDIYVSSYLPGQGINPGGNLWPITCSVIKTEMLPLFVQKFKSFAISQRPLASKYLPIR
                     HSCYSMNTGLADIDIRDFLEKAQTICDSEAQNLLNLWNTMVISSSFTNPDYTGNIGTA
                     AFWFPESRFNFENGWQRYFNLDFAKTRWLSFVNAALGNDNIPPQPPILNSQNLIYNTL
                     QLSFLASPDPDSLYYEISYDGFEHFQYIYPNPDNSIFNVMLQISKSGTYQLRAIDQNG
                     NKSEPVIGSYDYSTPNVSMLVNPNPISGTSLAVLRWWAGDELSGKIQLEIYNLKGQKV
                     LSKYLGEVYPGEGNFLLSSDQNFRALPSGIYFLKLQLGKRKLTKKMTILY"
     gene            complement(22486..>23520)
                     /gene="buk"
                     /locus_tag="PLB65_02900"
     CDS             complement(22486..>23520)
                     /gene="buk"
                     /locus_tag="PLB65_02900"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HOQ77504.1"
                     /translation="KIAVYDDSTPVFEKTLRHDPEDLNKYGDLFNQTDFRKTLVMEAM
                     EENNVHSQTLNAVVGRGGLVRPVSGGTWKVNDSMLRDLQDSTIWGRVHASNLGAFIAK
                     AIAEPLGIPYFIVDPVATDEMEDIARLSGIPEIDHKSLFHALNIRYICRLLAAELGKK
                     LEDVNLIGVHLGGGISVAAIKKGRVVDVNNALLGTGPFSPQRAGSLPIGDLIDMCYSG
                     QYSKKELTAYLSKGAGLMAYLGTDSGIEVAQRVSEGDQKAKNVLDAMCYKVSKEIGCC
                     AAVLSGKVDGIYLSGGLAYNDYIVEFIKQHTEFIAPLYVYPGEKEMEALCQGGIRVLN
                     GSEEAKEYPY"
     assembly_gap    23521..23620
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            23638..23976
                     /locus_tag="PLB65_02905"
     CDS             23638..23976
                     /locus_tag="PLB65_02905"
                     /inference="COORDINATES: protein motif:HMM:NF018633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HOQ77505.1"
                     /translation="MSDTEKTKCNHNGDHICDEECECNDNFITLEMDDGTLKNFFIIG
                     NLENEGKYYIALVAEGESNYDILQVTEEGDNVNLNYIDDDNEYNTIADKFDELFQKEL
                     KEMGIEEETE"
     gene            24527..27970
                     /locus_tag="PLB65_02910"
     CDS             24527..27970
                     /locus_tag="PLB65_02910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOQ77506.1"
                     /translation="MPFVHLHNHTQYSMLDGACRVDRMIKLAKKYEMPAVAITDHGNL
                     FGVVDFYKTARAAGIKPIIGIEAYIVNGDIESESSKNESRYHIILLAQNEQGYKNLVK
                     LSSISYIKGFYYKPRISKTLLQQYNEGLICLSACIKGEIPSLILNERINQAREVALWY
                     KDLFKDRFFMELQDHNLEPEKAVMPRLIDLAKELDIPMVLTNDCHYLHQSDYEAHDIL
                     LCIQTGKLLNDPGRLHYSTNQLYFKSPEEMKELFPEVPEAYANTLKIAEMIDLTLHYD
                     KFLLPNIETPPEFKTMGEYLKHLCYEGAKIKYPEMNETIKQRIDYELEVIDRMGFSGY
                     FLVVKDIIDNARKQNVPVGPGRGSAAGSIIAYLLNITLVDPIKYGLVFERFLNPDRIS
                     MPDIDIDFCAQKRGQVIDYIVQRYGRESVTQIITFSTLGAKSVIKDVARVLSVPASEA
                     NAITKTIPGNVKSLDDAYKQYPEFATLIDSNDLYRSIFKHSKVLEGLVRQTGVHAAGV
                     VIAPGDLTDYVPLACSSQKDEEKVILVQYEGKWLNDLKLLKMDILGLKNLTVIQKTID
                     LVKQSQNIDIDINLISLDDKKVYTLLGKGETDGVFQFESDGMRKYLMELKPNKIEDLI
                     AMVALYRPGPMQFIDSYIARKHGREKVVFDHPLMENTLKETYGVTVYQEQVMQIAREM
                     GGFTGGEADSLRKAIGKKSSELMQKYYEKFIQGAKTNNVPETVSEKIWQDWLHFAEYA
                     FNKSHATCYALIAYQTAYLKTYYPVEYMAALLSLEDDPSGIPGKIEVCKKMGIKIIPP
                     NINRSDSEFRVHNKEVQFGLRAIKNLGEAAMRAIIESRDQGGPYTGIFNFCSRLDSSS
                     VNKTILESLIASGAMDELEGTRAQKWAVIEQALQFSSNEQRDRKKGQSTIFDFLDEED
                     DNSNYYPPLPNVEPWGYLYQLEKEKEVLGFYLSGHPLYEYRSLIKHITNADSNTGKSN
                     NNGELYLAGIITNISKKKDSRGNQIAFVEFEDLAGKFEVSLFNKDYLQYSKIISPGKV
                     FFITGTKSTFNGNDDAMLRILPNSLIPFDALANELRGEIKVHINDKQVKKGCLTDLGE
                     RISRCDGKFKLITTIQKEDVNSYQLESRKLFFPGEGLLNWLEKEKIDFQIRLVTNGKS
                     T"
     gene            27954..29060
                     /locus_tag="PLB65_02915"
     CDS             27954..29060
                     /locus_tag="PLB65_02915"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOQ77507.1"
                     /translation="MEKVLSILILLFTFGFVSAQEIFYEHYANQVDIRVVLPYNNISF
                     KKGSDQAVYQIVLEISNSRKKQVATLEKSQIIPKYEWLTDCGIPVFFQLELPQGSYNV
                     ALKIKNKAMGDKRNYTRQFIIGPSSTEIGLSWVIAKKDGIDFIPYSLSGLSVDELCLN
                     QSYSLALDSLRIQIDNQNLTITNPQSPVIIDLLPYIQKDAPNQLALTFFENNIYYKME
                     PFLFSPWYAYSLRYSLNDQIRQIRYIANQNEWNVLRNVPEERQAEVIEAFWKANDPSP
                     GTIRNELRERFYQRVLAADELFTVHKKIAGWTTDQGRIYIKYGEPDDVYSEVFSIGKY
                     PFIIWYYYDKNLEFIFADIGGYGQYTLRNKDEEY"
     gene            29047..30384
                     /locus_tag="PLB65_02920"
     CDS             29047..30384
                     /locus_tag="PLB65_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HOQ77508.1"
                     /translation="MKNIRILICLALLSCFLIPLSGSEKLNMFIDYSRFLDKNKNTIL
                     LLDYQVPYQNLAFLAKNNGFFAELEVYIKICEGDSLLLDHKINDLIGVRNKADTSNKQ
                     KSYLNRFSFIMDRPICTVQFYAQDLNSEKSFMHEFEITALPPNSLLSDIELNSEVRAD
                     TTSYLPKFKRNNILYRSEPSILFNKTNTDYVYLYLESYIGSTSLESCLLNLSLEKDSV
                     IVMDEDLNYKPASSSESINLKIPISDLNPGLYLGTVILQNPDQYEERKFEFAVIEEKE
                     EEFFLFANPDDEFELMRIFMGNRISADWKDMSQDSKKRYITQFWRNMASNTNRSIQSI
                     MDLVQERIDYSNKHFGHFKKGWTSDMGRIYIRNGAPDDIQRETSSDESRFVRKDYQIW
                     KYSTGNKPVYLFIDIQMNGNYKLIYVVNDDMESSNPDWMRYVGYDFDTSKLDN"
     gene            complement(30381..30767)
                     /locus_tag="PLB65_02925"
     CDS             complement(30381..30767)
                     /locus_tag="PLB65_02925"
                     /inference="COORDINATES: protein motif:HMM:NF009154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOQ77509.1"
                     /translation="MIKYSLQNFSRIGEDLAARYLVTNGYSVVCRNFRKRCGEIDLIV
                     EKDQHLIFCEVKTRTSHSIESALSSVSFSKQRKITRAAQLYINENPNFGNYIFRFDVI
                     LVFYYESTDTFKVIHYEDAFPPLLDN"
     gene            30852..31970
                     /locus_tag="PLB65_02930"
     CDS             30852..31970
                     /locus_tag="PLB65_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77510.1"
                     /translation="MKKIILMAIAVVAVISSVQAAPFQTLGMLRTPDAYVLPNKAAEF
                     VLVGYYRDIAAPKPLTNDAKDASEGLFPYTMIGVGILDRVELGLFLGDYTSLDGLVYY
                     LNAKVKIIEETLRIPQVSIGMDNILSPVPKHGSQYLSPGDDFSQHPDADCYEPYSPYA
                     VASKQAVFLGIPWMFNVGIGTNRFVGQAKSRYLNGLFASAEMSPIKDLALQGEFDGED
                     LNIGAKYSYKNFGFKVGVNALEDWFKDSDYKDNIRIAFGVSYLFDKYAEAKRRPDLGH
                     YAGYGKTPSMGEGVVETPIETGITVPPTVSPSKPQAGTQTPAPGLTTSGTAGYEQLSP
                     EVQELMKELRMLKEDREKAQQAMDELRKWIEELKKQKQ"
     gene            32126..37975
                     /locus_tag="PLB65_02935"
     CDS             32126..37975
                     /locus_tag="PLB65_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77511.1"
                     /translation="MKKTLKIIFLTVVALSFVIVVFAQNSNEATKKEIIQQISQERQI
                     LKEIAKNFLIDVLPELVKKDPALAEQALVKYKDAYSSLGQNDIVYLLGHFYTRMGENT
                     RAISYFNSLLQTNLNEDSRKMLNLALYQQMVHYLETGNRKAARDFLQVIIFDQYQFDT
                     YYPTYLYVWADMTAEEGQYETVQNTLQSYMQNRDIIMNRLLPQKIAILARVQNLDLDS
                     YYANPSGETYKQLASQIDVIKGDLTNNYNELMSLKGIIYVDAIVNLHNEEIGMLDSLK
                     IVLSDYYTKKIYAEETITRGTSLLQQIKKLSSSYERQLAIMDQILERQYELYKANDPQ
                     IQGNKYSDMELKRLYDLEKNIQLYTDLIEELDNYIADPKLVDMQAQLKIIRDDYDEKR
                     TSLNLRKEYLLQVRKHSSDAQEQIFNTILQEYYALYPDKKNLDKQIMELEQFMKEETQ
                     NIFTEEMRNKIKSNVASHIAQTADSNKRDEPIRKNAIDLVANAEFIKLQLDYRNLHFK
                     EQQRLAQKETLTEQQLVELQAQIVAEKRDLITRIETFISNNPNFKAVEQPNGDFLVTN
                     ADLYYNLAELQYAAYLDNPSIALESYRKVVQIDPNYIDIDAALYNIGFISSEINHQRI
                     DSNKSRFYELNKTAVALDDNSKYKESYFAEAISAYQSIINNYKNSPYYEEAIYRLGIL
                     YYYIATDADVPQQYYELATNYFDTIINMPDSKYKYDAIYQRGWLRLNSYQEAELKLAM
                     NDFITLLNAVDNGKISDPAIAEDYRNDAIDNIAYCLIALDGTDFYSQAKGVTELQNIF
                     SGYQNTEVMNRIVDKAASNKFDLAASLQAIDFLWLKINMNPMALENPTLVDSILKIYA
                     SAQYQLREDQNFDQIAHELYQNIITNYGKNSTWYATNKDKNISAQLAVIQNAYEKRAL
                     RLHNEFLAEPTNEARLLAYYQHMDQYSNFMETHSENYPAWKAKTDKNKLLLSITLAER
                     SNLPKHYLSAIKCLQDFNTNYPQDEDFFLNEGLIYVYTNNIYNLMKDLYNENYNPEPG
                     LPANLDELFALLSTNSMRFINVLRSDAYKNPERESQAVTILLNLADIQYNRGKMPEAK
                     ELYLTALQNETLISNKTKFDIYGKLALISEQEKNFAQAETYYRKALPFASTPTEREAI
                     ANNIRYQIQNSYEAAEQAGNYSFAASERLRLAAELDPKDTQRIQGYKMAAQETYIKAK
                     EYQKAIDILLELAGTKTDIDEIYYYYFRAAEIAEADTAMNNPEIAKNIKDSFIAKYPS
                     SNQAFLLRIAMLQEIEKDPAQKNAAAEAYLALHEEARKKTINTGDITPDALLLKASIN
                     YREAGNKEKEIEIYNQFLQLYPNNENVIPFMQVIADDYLAKGDTLKFEQLAKQIYTKD
                     KTKGDRYQWIATTKLNKLMYDFDNAYKNKNYSEAFKYRDEYKRLEAAYKKEGLQFETA
                     DFSSAKNYEYFTSVENEYKAIEKRKAFLKNFDAQLTAIEKGNLMTSSPAKLIKVNVNT
                     TWQNHLIGGSYRRIPIFKESVRAEVAKVNKVLSSAEKENIELENYRRLRALNLIARIY
                     EKGVNVINTQIGVYVRTSYEATGVRQQYKDILDSFINSVAADQSNDLLSLAYTTHLNI
                     YNYYQMAGYTDYYTQQSYAKLQQWNLLPDYKIDEYPLGSGWSQKMDGNNINLPAQNVT
                     SPKGGRLGSIQIPAGKDIILSHTVNAKLVPDFALIQLVYPYDVNIRMNGTDVEAGVVP
                     IDTLDVNKPGTTTRYAYLLPSSSWIEGQNAVELTVPNNTPEPQNLYLSLQVFTDRQKI
                     AASIPPETVMLYTDPSWRIVNVNQETGTETFSAATITGNFGISLDNIEGIEGEQAKPI
                     WPVEEAPISSAVFEVDFYLDTEFREGLINFVAPETATVYLNGELLASNLAMDYDPEPF
                     RVYTTQVSIEPAKVTSGKNTLRFVVQNNSNYRGFIATVKIIKAGKEEVR"
     gene            37972..38274
                     /locus_tag="PLB65_02940"
     CDS             37972..38274
                     /locus_tag="PLB65_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77512.1"
                     /translation="MKKSFLLLAAMLFLAYIGYAQTVTPQPTNQQQPKGFEGVGQYVE
                     KEELIIEVKSLIPPTGVFQDKSDTYQKSGEYKFSFSLEDIRKQSDILLNKWLKESK"
     gene            38271..39038
                     /locus_tag="PLB65_02945"
     CDS             38271..39038
                     /locus_tag="PLB65_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOQ77513.1"
                     /translation="MKAKHIVLVIISLFICVGLFGQNAGEAAAPAAPASQGYSIVQVF
                     VDSGNWAYAILLVFIIGLVYAIVRWNQLYRKEKIDAGGFYLKLRNYIRNDQLEEAAKI
                     AESYKGTTMGFIFWSALMIFKDAKKSGQSGNDLRMAVQNSVEESVLQTVHKLDSGLFW
                     FDTLAQVATYLGLLGTIWGLLEAFASLSGLTGAAQQTALTDGIKKAIGTTALGLLAAI
                     PLTLIKGWLLTRANNVISDIDEYSVKLINTINNAIKD"
     gene            39043..39564
                     /locus_tag="PLB65_02950"
     CDS             39043..39564
                     /locus_tag="PLB65_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77514.1"
                     /translation="MERYRPSAMRQQKSQTIPQEPNLVPIMNLFLTIIPMLMMMVVIS
                     QIALLALNFSQGEGGVTTGGGGGGGGPVTEKVEIHILGKNYAASTNFLGMEIREPGVA
                     TKNIPAINGFYDYPSLDKALQEIRSRKGNLNDITVLVHPDVLYGDLMKTIDLCKVHGF
                     SQVHYSAPKVQYY"
     gene            39576..40124
                     /locus_tag="PLB65_02955"
     CDS             39576..40124
                     /locus_tag="PLB65_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOQ77515.1"
                     /translation="MHITDKLSSSNKSARRVRRKFEEAKSLSITSLLDELTIILVFLV
                     KNISTEAVKISSDPAIQYPTTITNDELLAKGVATPIKIFPDRIVVGTEALEYGSPADL
                     LENPKKRQDLITYLSAEVNSILQQKDAETCLLVQADYSIPCDYITEIVRAGTAAGYSY
                     IYFATLEDADWLQKYNLSLAQQ"
     gene            40134..41561
                     /locus_tag="PLB65_02960"
     CDS             40134..41561
                     /locus_tag="PLB65_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77516.1"
                     /translation="MSKLLNLTLHYKGKVLDRVRYCRDFKNKFFIGSDKYLFWQILDS
                     SFPEKHLFVTKKGDQLYLQLPPGSKVSCTKGGQPVDSAYLTKNNILKDNRLLLLPDMT
                     GTLNIAPNWEISYEFKEPFVTVLTPEEKQIASQYARRPQPTSTERFNRNMILLFIVLT
                     IIFVIIFDQFLKKQISYDYTLEQKVAMLQKAEQIKAEALAKPSTFEPTGETPEAVTEK
                     AQQGVTGGAIGGTGTAKSAGAIFGGGFGSFDPSATSAARPIQAVTTAESFVTSRPGRG
                     GGGGGIGTGGGGAGPSGGYSTSFDPNAPRTFSSDIGSVATQAPQVAGSSIRPGVGTIA
                     KATGDQSRLAPSGVVFGQTAQSSQLISSFQSKKVAQVSEGTIAQAPAETKTRYETIGA
                     LVNSRRSQINTLYSKWNAIIPFSGSVTIRLLISSSGKVQEALITPNGQIPNGFLTELK
                     QLCESWKFPVTEESDYTFSVRLRQA"
BASE COUNT        13545 a         7788 c         7422 g        13033 t
ORIGIN      
        1 tctgttcttt tccttgatac atacggaggt ctgtggaact gcatacttgg ctttgcctcg
       61 ttcaccctga ctgcgtctca gctccagaaa gcgctcgacc ttcttacagc tcaacacaga
      121 ataataaaat gaactgcttg atattgtata tctatttgga attggctaag tactttcttt
      181 ggttttagta cttcctgctt atctttacat ttcttacatt tttcctttct gtgcttttgc
      241 catttttggg ttagtgacat ttattatctc tggtaggggg gtacttatac cctctactcg
      301 ccataaattc attttggtta aatcacatct atatacattg ggtagaagct aacaaggata
      361 aggtattaga tatgtttacc gcacagatta tccaataccg tttcggcgat tgctttttta
      421 tgtataattt atatggtgta ctttcctaat gctgaggaaa gataaagaag ttactgttat
      481 ctaatataag agctgcaata tgtaatttaa tatgccagca ttaataagga ttctacttcc
      541 acagcagata atttactaac cattccgatt ctgggactac tcataatatt attaactatt
      601 tgtggcagtt cattttcctt aatacccagg tctcgcaaag tttgtgccat tccccaagat
      661 tccaatttat cgcgaaatct atgtagagcg cgggaaacat tttcttcatc ccaaacattt
      721 ttagcccagc gtaaaaagcg agaggggtct ttttcactca tatattcaat ccaagcaggg
      781 aaaataacac ctaaaccttc accatgagca atatctggat gtaaagcaga gaaactatgt
      841 tctattgaat gacaagccca gtcgcctccc ttcattccga ttccggaaat tccatttaaa
      901 gccaaagtag cgcaccatgc caaatttcct ctggctagta aatccccgga attaagttgc
      961 aggcggtcag tcatttctat aattgttttt tgcagagcat cgtcgattgc taaggtagcg
     1021 atagctcgat catcggcaaa gtaaaattct aaaatatgag caatggcatc cattgctcca
     1081 ttagcagttt gtttgaaagg taaactactt tgcacagaag ggtctataat acttaaatgc
     1141 ggatagatca acggggaata aatactccat ttttgtaggg tttcgttatt agtaataacg
     1201 gcatttccat tcatttcgct gcctgtggcg gaaattgtta aaactgtata aattggtaga
     1261 gcattacgaa ttcgttcctt tcctaaaaaa gcattccaga tattttgtaa atatacacca
     1321 gcagcaacgg tctttgcagt atcaattaca cttccacctc caacagcaag aatggcttcc
     1381 actttttctt cttttgccag ttcacagagt tcattaacct tttgtaaagt aggattagat
     1441 tgaacccccc aactttcaac gaaacggatt ccggatttat ttaacgattc acaaacctga
     1501 agataaacgc catttgcctt tattgaacct ccgccagcta ccatcaagca gcttttgata
     1561 cccgctttgt gtagttccat tcccaatatt ttaatctttc ccgttccaaa ttcaatccgg
     1621 gtaggattac aaaaactaaa ggcctccatt tctatctcct tcatattcct ttttgtgctt
     1681 gttgttcaga atctaaatac aaaataacgc aaatctgtaa taaaacaagt aaaaatgccc
     1741 atttttcccc tgattccatt ttaaaagctg aaatttttta ctgtttagga gcttctctaa
     1801 cagttcctaa acgagattaa gagataatta ttgaaatttg aactcggcat acagccagaa
     1861 aggctatagg agatagactt attcttatta agcagaaaaa attatggtgg agctaagggg
     1921 attcgaaccc ctggcctaat gattgcgaac cattcgctct accaactgag ctatagcccc
     1981 aacctcgttt ccaagaaaaa ctttctggta gcagtagttt tgtcaagcga aacttgagct
     2041 cttctatctt gtaaatacta ttgtaatacc aggaaaatat tagtacagct ttatctctcc
     2101 actgctaact tttccttgga ggtaatagga ttattaatgc atctggtaag aactaaccct
     2161 ttataattta cgcatatact ccagtaaatc taccactcgg ttagaatatc catattcatt
     2221 atcataccag ctgaagacct ttaccatttt ccctttagtg taggtgatca gagaatcaaa
     2281 tatggaagaa tgaggattac caacaatatc tatagagaca attggttctt cagtatacat
     2341 cagataaccc ttaagatagc tattggcagc ttcattcatt gcagaattaa tatcttcctt
     2401 agtaacttcc acttccaggt tggcacagag atcaactaat gagccgtctg gagtaggaac
     2461 tctaatggcc actccatcca gtttaccttt taattctgga ataactaaac cgatggcttt
     2521 agctgctcct gtagaagttg gaatcatact cattgcggca gctctggctc ttcttagatc
     2581 attatgaggc agatctaaaa ttctctgatc gtttgtgtag ctgtggatag tagtcattaa
     2641 accatttaca atgccaaatc tatcttgaag aacttttgct acaggggcaa gacaattggt
     2701 tgtgcaagag gcattggaaa caattaaatg ttctgctttc aaggaagtat gatttacacc
     2761 cataacgatt gtagcatcaa catcatcttt cgcaggagct gttaaaataa ctttttgggc
     2821 accagctttt aagtgtttcg atgccttttc cttggttgtg aaaataccgg tagattcaat
     2881 tacatattcc actcccaaat ctttccaggg aagcacttca ggatcttttt cggcaaaaat
     2941 tctgatcttt ttgccattca ctaataaata gttctcttcg ctggaaatct gtccaggata
     3001 aactttatga acactatcgt atttaaaaag atgtgccaaa gttttagcat cggttagatc
     3061 gttgatagcc actacattca attccggatg gctgttaatt attatgcgta aaaccagacg
     3121 cccaatgcgt ccaaaaccgt taattgctac atttgtcata ttctatctcc ttcagttata
     3181 gtaatatact attttgtgcg ctaccggaaa tattgcagaa atccataact gtgttctgca
     3241 ttttttcttc acctttaatc agccatttgc gagggtcaaa tttacttttg ttaggaacat
     3301 agagatttgg atccatatct ggagggcaga cgattgcata cttttctttt tcccaatatt
     3361 cttgaatagc agaagccata tccatttgat aatgggtatc tttattgatt tttactactc
     3421 cattggcaat agcagttttt tgctgttggt ctgttaaccc cgaagcaccg tgtaaaacta
     3481 atcctctttc tactccattt ttaattagca ggtcatcaat ttcttttatt aaatcgaggc
     3541 ggagaacgat attttctcct ttacaaacgc catgattggt tcctactgaa gccgcaaaaa
     3601 ggtctacatt tgtgcgtttt acaaattcta aagcttcttc tggtttagta taaagttcat
     3661 cttcggaaac aatttcatct tcagcaccct tgatatggcc aatttcacct tctaccagaa
     3721 tattatattt atgagctaaa tctacaacct ctttagtgat acggatatta tcttccagat
     3781 tttctttgga agcatcaatc attacagaag ttactaagtc gttttcaata caaaacttta
     3841 tgaaatcaaa atagttaggt gtggcatggt ctatatgtaa accgattcca catttaggaa
     3901 attgggatgc aaaggtctta gccattgctg aaatcattct ggctccaatt tgtatatctt
     3961 gagaggaacc tgctgcatat ttacaggcac ctgtactcat ttgaagtaag ccatcagaat
     4021 ttacagctgc ataaccttga ataagggctc ggatttgtgt gtcgaagacc acattggaag
     4081 cgataatccc aaaacgcatt tttttggctt ttagaaaaac ctctttcatt tgtaaacctg
     4141 ataaaaacat tttatacctc cagcataata aaattcttat tagataatct atatatcatc
     4201 cacaaaaagt caagcaaaat ctaaaaaagg gcaaaaaggg aaaagtaaat tctctgtttt
     4261 cggaagagat aatacccaat cagcagtatt gatgctcaat aaaatgctac tttatcagaa
     4321 taggtgattt attaattatc agtgctatct ttcaggatag attttatgac ccaggaagaa
     4381 gccatcattc ccataattgc tggtatataa cttattgaac caataggtat gcgttgagag
     4441 ttattgtaaa cgatttttgg ggaaatattt gggtgttcaa ttgaaggatt attacttttt
     4501 tgattgggtt tgataggaag ctcagaagaa tacacacaag ggaaattaat ctcaatctct
     4561 ctttgcttta atagcttacg aactctacgc gccaaaggac aattatggct cttatccaaa
     4621 gtggaaatat gaattaaatt aggatccaga cgatttcctg ctcccatcac actgataaat
     4681 ctcttcttat ttctaaccag aaattccagc aagtcaattt tggagttcaa actatcaatg
     4741 gcatctacac aataatcagc ttcagcaagt aactgttctt gattatcttt gttcagaact
     4801 tcccggtgaa caacaatttc cgcttgtgga ttgatatcca gaattcgctg ttgcataagg
     4861 tctgctttgt atttaccaat agttgattgc aaagctaaaa tctgcctgtt tacattgctt
     4921 ggctcaacta tatcaaaatc aactaagatc aatttaccaa ttcctgaacg agctaatgct
     4981 tctgcagaat aagaaccaac tccccccaaa ccaataactg ctatacaagt tttggataat
     5041 gcctgcagtc ctttttcacc aattaaaagt tcagttctgg aaaaatccgc tttgttcata
     5101 gccattaaag tttctttgct gttctgtaat taattgctct aacataattc ctgaaagatc
     5161 agctacagaa gaaacaagtg aagataagcg caaaagagga ttcttttctc ctacttttac
     5221 atattcacga gtgatatctg tttcaaaaag atagcgttta ctatctatca tagccagcaa
     5281 aagttctttt ttatccttct tcaataaccg ctcagaaata gtaaacagag tatttaagcg
     5341 taaaagttct ttgtaacctt gtagggaacc tgcataaccg tgaacaagat attgcagatt
     5401 atattgcttc aggattttat aagcctcggt tgtatgtcct accagatgca aaactaccgg
     5461 tagattatat tgtttggcaa gttccaattg ggcaataaaa agctccacct gacactcata
     5521 atttggatta tttctatcca aacctatttc accaattgcc cagattcttt tcttttggca
     5581 aaggatttct atatcttcca attctaaatc gcattcatca taacaaggat gaataccagc
     5641 actgtaagca attctattgt cagggttatt ttggtgccaa gcaatttccg attgagtaag
     5701 cacagatgaa agccaaaaaa agatacccag tttttctgcc tcttccaata atggctttac
     5761 tggcattaac tgattcagat tagccagatg gcaatgtgca tcaattaaat tcattattcc
     5821 ttcttaaaaa aagtagattt acagcctcta aaacaggttc tacggcaatg agtttcatac
     5881 agttatgatg atctaaagga cattttttag caccatgact accacagggt ctgcatttaa
     5941 gatcaacttc aaaaactttg tctccttctc tatatggata ataaccaaat agaggaacgg
     6001 taggaccaaa aaaagcaaag accctggtct gcatagcatt ggcaatatgc atagcgccac
     6061 tatcattaca aatcattaaa gagcattttt cgataatggc agcgctctcc agtaaattag
     6121 taactcctgc aagattaatt aggttagcat tattcttctc tatagcaatt tctatttcat
     6181 cacattgagg tttatcgctt tctcctccaa ttaaaacaat gccatatcca ctatctaaaa
     6241 gtttccggca aagtgagata taggaattta gttcccagca tttagtcttc cagattgaac
     6301 cgggagcaat agcaattaat gtctttccag ataatgcctt tagcaattcc tccgctttat
     6361 gtttatcatt agctgagggg aaaagctctg tctgaatatc gaattcctta ttactgaggg
     6421 gttttacgag gcctaagttt ttaattattt tatgcggtcc cactggatgt tctacttttt
     6481 tggtcagcat ccatttggca aaacctctat caaaaccaat tctttccgga attttactta
     6541 aatataggat taaatgagtc cgaaatgagc tgtgcggagt gatagctatg ttatagtgtc
     6601 tatttttaat atagcgtatc aactgcacag tagattgcca cacatttttt cgcttggaat
     6661 cgataatcac ttcattcaca taaggattat tttccaggag tttagcggct tcgtttacta
     6721 ccaaaacatc aattttggtt ttattaaata gctttgctac tgctcttatt aggggagtta
     6781 ttaaaattac atcgccaatg aaagcagttt gaacaattat tatgcgttcc ggttctttca
     6841 taaggtttta taaagctcca agtttttatc caccattagt tccttatcaa attgttttac
     6901 tgtttctaag gcattcttgc catatttctg cctcagacga ggttcactgg ccagtttcaa
     6961 gattgaatgg gatagttcct gaggattttt aggggggacc aataaaccat tttcttcatt
     7021 agagatcatt tctgaaattc ctccagcttt tgttccaatt acaggaaggc ctacactcat
     7081 tgcttccaaa acggaagttc ccaaaccttc taaataggaa gctagcacaa agatatcaaa
     7141 cgctttcaag aaatgtccaa tttctttttg aaaaccggtg aagctaatat gaccctgcaa
     7201 accaaattcc atagctactt ttttcatttc tggcaggaga ataccatctc cgacagccat
     7261 aaaaaaaagc tctggattct ctttaacggc taaagaggca gcttgtaaaa aattaggata
     7321 atccttatga ccggcaaaag cagcgacagt tcctacaaca atactttttt caggtatctt
     7381 ccaagtttca cgaaagtttt ctggaacctt cttgtctgcg aatttcttta tatcgattcc
     7441 actatggata acagtaattt tagactcggg aacaccacag tccacaagaa cattgcgaat
     7501 attattcgaa acagtaacga ttttatttat tgctttagtt ctatacttaa atgcagaaaa
     7561 gggattctta ccaataggaa aatctactcg tctggtagct acaagtttta agtgaggaca
     7621 gaataatttt gccattaaac cccaactgag agaatggcta ctatgtaaat gcaaaacatc
     7681 ataagatttt ttccggcata gcatagctaa cttaaaacct gcatacagat caagctcccc
     7741 tttacaagaa attgtgtgaa aaggcaggtt atttttggca agataattat gtaatgagga
     7801 ttttggtaaa caaagaaagc cacaatcaaa acctcttttt agcatacctt cgtaaaggta
     7861 aattgcctgc tgctgacctc ctctccaagt tttttctgaa tctatgtgta aaactctcat
     7921 ttttgcattt ttcctaatag atgatagcga taccagattc cccaggcaga atttttgcag
     7981 agcaaaaagc ccttcgctcc atccaaaaaa cccagctgga agatatacat tttgaaaaac
     8041 ttacatatac ctctaatcca agcagaaaac tcattggact ttttaccttt ttcataaagc
     8101 tgtttagcgc taagttcagc ataaaaacgc attttagtaa aatgtacttc tcgggtggga
     8161 tatgtataat gatatagata atgtttacat ttttttattg gttgtgaggt ttgaattcct
     8221 tcgtggacta tttttgtgtt aaaactaccc ttttttctat taaataaacg cactggagca
     8281 tcgttttgcc aaccacaaaa atgtatcggt ttgcctaaat aataggaacg cctctttatt
     8341 cgccaggcag agttttcaaa actattgtgt tttagtgtct gtagttcttt ttgcagttct
     8401 gggctaattt cttcatcggc atctatagat ataatccaat caaatgaagc aagatcaacc
     8461 gctttttgtt tggctttacc aaagccctcc catttatcta aatgataaac tttagctcca
     8521 aaaccaacac aaatattctc tgttgcatcg gtggaacctg tatctaaaac cacaatttca
     8581 tccaccccag aaagtgattt taggcagcga gcaatattat ttgcttcatt ttttacaatt
     8641 agaacagctg atattttatc catttaactg tttttacctt ctactactat ccttaaatat
     8701 tcagcagcaa aacgatcata actaaagtgc tctttcgcat attcatgggc agaacaactc
     8761 attttttgta ataaatccct attcacatta agttccttaa tatatgaagt aaattcatct
     8821 attatcttct gttcgtcttt ggctttaact aacattccat ttattctatc ctgaaatgtc
     8881 tcttttactc ctccaacatc agtggccaag ggaattaagc cacaagtcat cccttctaca
     8941 ataactaagc caaaaccttc agatactgag catagtacta atatatggaa attctggata
     9001 atttcgtata acatcttccg actaacaatc tcccccaaat tgataaccgg ttctgtttct
     9061 acgaagttac caatgcagta gaactttata tctggatgtt ctcgaataac attttgagcc
     9121 agctttcggt ataaatagaa tctcttttct tgtgcatttc gagcaataaa gacaatttgc
     9181 aaaggggcat aataattctt ttcaggaatc aaatcacata atggaactga attgtaaatc
     9241 aactgtaacc ggcaatcata aatactatca acaccatttt ttctataaat atcttgaatt
     9301 cttcccaata tactattaga aacaactatt cttctatcta ttttgggagt tgcctgaagc
     9361 caattatgat gatttatacc aaagagacca tgtataatat caattaaata actatcatta
     9421 aggtgacaaa ctaaccgata aaaaaaggca ctattagaag acatcacaat ggatttgtga
     9481 tgtctattga ttagtgaaga tagaaagcct aagaagaaat acttaacgat aaaacgattg
     9541 gcattattca ggtatttaga catatctatt aatttaccac actcttgcat ttccttataa
     9601 tggactttat tgcgtgattt attggtaatt atgatataag gtctgtggga agcaaaaata
     9661 cgaagaatat cgatatgtat ttgttccgca cctccagtat gataatgagg aaataaaaaa
     9721 aagagtgaac tactattatt ccacttcaat atcttatcag ttaatgtgcc tgtgtaaaaa
     9781 aaggggagac ctaataatcg ttctaagttg ttcatttcaa tatataaaga tcattgcgct
     9841 tagagtttag ccccttttgg catctatagt tatttgggaa aatctcattt ctgcgagatt
     9901 tactataacc agagtttttt ctttcgccct tgttatgata gggttgatga tattgatgta
     9961 aagggagttg agaaagtaca aagttaaagc caattcttcc taatgcatgt agtcgttttc
    10021 ctaaatcatc atcctcgttg ccccaaccga tataattttc atcatatcca ttaaccgctt
    10081 caaaatcatt tctaaatata gcagaaacac cacctcgcat tttagcgcca tgttctacaa
    10141 aaggtaaata cttgcagagt acataggata aatagtcctt tctatattgt ttgataattt
    10201 tcttcttctg cgcctcagtt aaaatagcat tccagttatt ttgttcaatc atctcaaaag
    10261 ttaatttttc tgtttgatct ttactaagcc gaacaggata accgcttaag aatctgccta
    10321 tggtaagttc atttttaata gatagtaaat agttcttggg tataataata tcctgatcca
    10381 gaaaagcgag aatttcacta ttgcaaacag aaacaccatt gtttctaact ctggcagccc
    10441 gaaattgctg atgttcttgt ctaaccaaga tcattggaat tgaagttttt tctttttgca
    10501 cctggaaaaa gccaattata tcttcttctg agccatcatc agagaggata atttcatcag
    10561 gttgcaccgc ttgtctataa accgagaata gacatttttc caataaatcc agataattgt
    10621 atacggggat aattacactt aaattcatct tttcagcaaa acataattct tgaaagtgcc
    10681 gatttgtttt ccaccattga agtgtttttc gataaaagtt aaaaagcgat atttaaattc
    10741 ttcatgttta tgcagttcgc gataaggatt gggtttgccg gttaattgta atttatcttg
    10801 ccaattaaaa ttagttatca tattttgcat cactattgga tgagttcctt caaaaaaagc
    10861 caattgagaa aggggtccat aatcaaaata cttaggagct ttgttgtaat attcttctgc
    10921 tttttccttt ccccaatgga cagaatttaa tgccttgcat ttattttgca tcaaattggg
    10981 aggacgcacc cagccgtaat gataaatttc agcatccact ttggcaacct ttaatttcct
    11041 ggttccggtt tcctgacgcg gattatcata atattcaaaa taacgaaagg attgagcact
    11101 ttggtatgaa tgaatattag gattatttct aattatgcgt atttcataag gataccagcc
    11161 atggccatta tgatagtgat aataatctcc ccaaaaatgt ttataagcaa aaagtaatcc
    11221 ttccacttct ttgtcgttta acagttcttc acagcgtttt tggatcacag gtaagtattt
    11281 ttcgtgcaca acttcatccg cctgcaaata aaaaagccaa tcaccagtgc attctttttt
    11341 ggcaatatcc gtttgccagg aattaatcat cccccgtttg catagtttct catcccaaat
    11401 ggtatcgatg atcttgattt taggattgtt caaggctaaa atgcgttcct ttgtatcatc
    11461 atctgcatca cctttcccga cagcaattat aaattcatcg cagatgggca gaatactctg
    11521 gattgattct attactggat agtagagttt aattccatta cgcacaaaag agaaaccgct
    11581 gattttcatt gttacctcat atacctaaga tctatacata ttgctatttc ccgttttgta
    11641 aattatatag atgttgatat cgagaacagc tttctaataa ttcctgatga gtgcctattc
    11701 ctacaatctt tcccttttcc aaaaccacta tttgatccgc tttcaaaata gtagaaagcc
    11761 gatgggcaat caagattaca gttctgttta gcgttacttt atcaatggcc aattgtactt
    11821 tctgttcaga atcggtatcc agagcagaag ttgcttcatc aaaaattaaa ataggagggt
    11881 cacctacaat agcccgcgct atgcaaagcc gttgtttttg gcctccggat agatcggaac
    11941 ctttactgct aagcacttct tcatatttat taggtagctc atcgataaat tcatctgcat
    12001 aagcaatttg tgcggctttg atgatttgtt cgtcagtaac ttcatcttga gaaccataag
    12061 cgatattttc tttaattgag cgcgaaaaca aaattgattc ctgagtaaca actccgaata
    12121 gctttcttag gctatccagt ttgatatctc ttatatctat accatctatt gttatagcac
    12181 cagaattaac atcatagagg cgattcatta aattggcaat tgttgtcttt ccccctccac
    12241 tggaaccaac aaaagccact ttactgcctt tggagatggt taaattgaaa tttttcagta
    12301 caggcttagt attcttatag ccaaaagtaa cattatcaaa gctaatttcg cgatcaaaac
    12361 tatctttcac aatagcatca ggtttatctt gaatgcgaga ttcttgattt aataccaaac
    12421 ctattctatc cagagaaaca agcgccttac ggatatctgt atagacttgg gtaatcactt
    12481 ttagcggatg tagcatagaa aataaagcaa agaggaaagc agtaaaatcg cccaaagaaa
    12541 aattgcttcc tggagccaga atcatattgc caccaatgat aataactaag ataccggtta
    12601 tagcagtatt cagctctgca ataggtgtgt tcaatgctcc ataatattgt gccttatacc
    12661 acatacgata atgtttttgg ttgatggatt caaagatttt tttctcatag tcttcccgcc
    12721 gaaaggcttg aacaactttc atactgttta gtacttcttc tacattggaa aagagattgg
    12781 agatttgttc ctgtatacgt tttgaatatt tctttatctt cttgcctaac agacctatcg
    12841 aaaaagaaaa aaccggtaga actatgatgc tgtataaaag cagagttgga ttcaataaat
    12901 aggctatccg cgcaaacaca agaactgtaa aaatttccct agttacattt acaaaggaat
    12961 tgatcagctg ttcacttacg atatctacat cccctaccat tctaacaatt gcatctccaa
    13021 cccgatggcg accaaaaaaa gtgagggact gatttaaata gcgagtaaac atataattac
    13081 ggatatcgcg gatcgtttta cctctcaaag aattaaaaaa tatcttatgc agtaaataaa
    13141 aaccattctt taataggata ataacaaaaa ttgctacaca taaaccatat aacagggaaa
    13201 gagaatccgt ctgcaacata agctttttgc agccttccca caaggaattg taattctgga
    13261 tggcagcaaa aaaacctcca gacgaaccga aatgttgctt tattaattct cccaaagcag
    13321 ttagaaatcc gctaaaatct gtatatagaa gattgtttct gcctggttta aacacataat
    13381 caaagagagg aatagctaaa gtaacgctta ctccattgaa aagagtaaaa aatagcatac
    13441 agataaaacc ggcaaccaaa taatgccaat aacgcaacat catcctgaaa atcaggataa
    13501 tattattcca ccgcttctga tttttccttt gtttcgccat aatttaccta taaaaaaaag
    13561 ccagcaaaag ctggcaagaa aaaagtggca cgccctaagg gagtcgaacc cctaaccttc
    13621 tgatccgtag tcagatgctc tatccaattg agctaagggc gcacaataag ttcacaaaat
    13681 atattacccc gcattctgtc aatcatttct tcttattcta tatataccct ctatttcttc
    13741 tcctattttt ggtgagaatc caatggcttc ttatagctaa ttatctatac attagtgggt
    13801 agtgggcata aataacagaa gattttaaat gcatctcgtt ccattaatac tcaatattcc
    13861 attcgcagat cagtaaagac tcctaaatat cgttaagaaa gccttccatg gctgaagcac
    13921 tcttttgtga gattgctgta catctattaa ttatattcct ttaatccctt atcccaaaaa
    13981 cagttttatt aagatagcga tcagcaagat agaattgagg cataaccaga ttcgtatttt
    14041 gtttaaactc tttgtgtata attctctatt atgctgcacc tgcttttcca gacgcaccat
    14101 caaatgttcc aaagaaccca gtttttctgc tgttctatct ttcttcttta tatcctgatc
    14161 gtgttttgaa tttcgtttaa atactctcat catttccatt actattggaa taaaggtcgg
    14221 aatcattgtt ttaaatttca tacccatttt tttatcctca tttaaaatta tatcccagtg
    14281 aaaaataatg tgttccaggt aaaacagaat ggtaagtata agaataatcc aattctaaat
    14341 ctcgaatata gatactaagt ccagcattgt aagtagcagg attttgatga accccgaagc
    14401 gtatagccaa aggttcaaaa agatgagctt ccacaccacc cataaattcc gtttctttgg
    14461 caaaatcctt ttttacttct atggaagtag ttactttatc gtaaggaatg taagatatgc
    14521 ctaaagccaa tttactgggc agatcaatct gattttcatc tcccattttg gctttattga
    14581 tattggtaac ggcaaaagct aagcgcgttc tagtatgtaa aactgccata gctcctaaat
    14641 ccagacctag ggcagtatca tcatcttcac catccatact taggagataa aaattacctg
    14701 tataaccaaa agatatttgc gaatggatat cctgcaacaa ataaaaactg tgcgccaaac
    14761 tatagacttg ttcactcatc agatttacat cttcataatc aacatccata gccctgatcc
    14821 ccaaagctac tgtgcccaat ttttggggaa gcttatagcc caaagccacg gttttaagtt
    14881 cactgaaatc ctgattgtat agctgagaaa aaccaagctt ggcactcaaa gtagtattta
    14941 ctaagcctgc aggattataa aaaatggcat ttgcatctct ggaaacagca gtataggcac
    15001 cacccaatcc tctggcaata gctgaaggtt gataatcatc atacacagca agtaacattg
    15061 ttgcaggaat taataataga caggcgaata ataacttttt catttctaac ctcacttcag
    15121 cgcactctta ataacaatgg gctgaacagt attgtcttta tctccgctac tccgatcgat
    15181 aacttccaga tgacagtaat ataaacctgg aggcagtaac tttaaattgg catcgcgtcc
    15241 attccaaata aaattctcga tgcctgtgga agaagttata ttagtatgaa caggagtata
    15301 aaccaagcgt ccttgcgcat cataaattct tacaattgcc ttggaaagat aaccggtttt
    15361 agttccataa ctaattataa tctgttcacc catttcagga ttgaaaatat tcttcttatc
    15421 gccaatttta ctaatatcta aataagcagt cttcttttgc agtttggtta ccagggagcc
    15481 atcattcagg ttgagaacat tggtatcgtt atatttaaag gaaacaagtt caataacaga
    15541 ttctccataa accaacggtt taaccagaat tttaattaaa agagcatcgt tttgattata
    15601 aataaggcca tcctgataag taacagtata gctaattgtg tctcctgcac tactaatata
    15661 ataatctgga atggatggag ttaaagttcc ttccgtatca aatccagaaa aatgaatgca
    15721 agcaggattt agcttgaaac taacagaata actttgcacg ccccattccg cttttatttt
    15781 cgtgcttatc aaattcatag atacatctgt attaataagg ggatcggtag ttggatcagt
    15841 tctcacaatt ttaataatgg aattttctat agtatcctgc ttaatcatat cattggcatt
    15901 acgcggattt aatttaaaac ccgcactgct atgaaaatca acaatgccct gaataatata
    15961 ccaactttca ccaataacaa ttgaatttgc tggaatagca tatagtccat tatctaccat
    16021 tgcccaaata ttgctgctgt cggctatatt gaattgtccg taaccatcag gagaagattt
    16081 aatcgttaca tcctgaaacc ttactaacac tccttcatac ttttcatttt ccggatgatt
    16141 cgttccggaa ggcatatttg ctgtagataa ttccaaaaaa ggcggtaagg catttccttg
    16201 agatattatt tcgtaagcag atacattggt aaattcggtt aagttaaaat actcatcaac
    16261 cgttcctgta agctttacta aatctccgac acttacaaca ttgctggtat ttctatgata
    16321 aacataaagt ccactccatt cacctccttc cggatcagca atataaaaag cggaattagg
    16381 aatgatgtga gtaactattc cttgcacagt aactgtttga ttatagtaag gtgaattgcc
    16441 tgtaggatca gttgtatatt gaatatcata acaggttaga actgcttgag ctgatatacc
    16501 tgttataaaa acaagcgcaa aaaaagttaa aagatagttt ttcatttaca gctccttatc
    16561 tgattcattg atgattagtt gttcatcttc ttcaacagaa tcactggaat tatctttgat
    16621 gaacagcata tcaataatta ataaacatac accgataaat attgcactat cagcgatgtt
    16681 aaacacagga aaacgttcca taatgaaatc aggaaaatcc attgaaaaga aatctgtaac
    16741 aggaccaaac aaaacacggt ctataagatt acctatcgcc cctcctaaaa tcaaaccaaa
    16801 ggcaataact tgaatctgat gttgagcgcg gcgtaataga taaatgataa gacctaccac
    16861 gataactgtt atacttataa aaaatatcct gtttaccaga tcatttccta atccgattga
    16921 aaatgcagct cctggatttt ctactttgat aagtaagaaa gtatcgccaa aaagaccttc
    16981 cagtaaaggg atgtgttcat ataatttcat atttacacga accagagtct tagttagctg
    17041 atccataaca acaattagca tcattaaaaa ataagcagga gcggttttta gctttgtaag
    17101 gggaggtttc aacgcttttg ccgccttttt ttatcttcgc ttttttcttg acaggcaaca
    17161 cagcaagtag cataaggaat aatttccaaa cgcgtttctt gaataaattc accgcaaatt
    17221 tcacagactc cataggtttc atctgctatt cttcgcaggg cttcatttaa ctgacggatt
    17281 ttttctcttt cgctctctaa catcataact gtctgttcca taaggttagt gtctgaacct
    17341 tgatcagctt ggtgatatgc ataactggat aaatcaccac tgctttcacg agcacctaag
    17401 ctctgttctt tattgatatt attaatataa tccaaactct cccttaattc acctctaatc
    17461 agttcttcga aatgccgtag tttgtctggc gataattttt ttgctgccat ttttatctcc
    17521 ttgggctttg catttaaaaa acctaatttt tcattaatta taaacctact gcttatgtca
    17581 atgaaaaagt ctttgttaca cgaaaaataa actctgcaat tattttctgt aattgtcggc
    17641 cagagttcaa tgccgttttt tgaatctctt cctggctatg tgccaaatta tgaaaaagat
    17701 tactgtaatt agtaactatt gaataggcaa gaacttttat gcctgaatgt cttgctgtta
    17761 ttacttctgg aacagttgac atccccacta aatcagctcc taagatagca aaagcattac
    17821 actcagcttt tgtttctaag gagggtcccg taacagcaat atatgtacct ttaaatgtgc
    17881 taatttcaag ttccttggca acctcatcgg caagtgaaca taattctgga tcataaggat
    17941 tatttaatga aggaaagcgc tcacccaagt tatcatcatt tattcctatt aaaggattag
    18001 ttcccataaa gttgatatga tcctgcaatt gcactatcgc tcctggctta agttcttttc
    18061 ttaaactacc agaggcatta gtaactatta aaacttttat tcccaaagaa gccaaaaccc
    18121 ttactggaaa aacaacttcc tgcattgaat agccctcgta gtaatgaaaa cgaccctgta
    18181 aaaaaataat gggaattccc ttcacctcac atagaagtaa attacctgga tgagaaggag
    18241 aagttgtttt tacgaaaccg ggaatttgat tatagggcaa agaacataga acaggaaaag
    18301 cttcaggtaa atcacttagt cccgttccca gaattactgc gattagaggt ttttcggaga
    18361 acttattttt caaccagatt gcagtttctt ggtaattgtt cacttattga ctctttgtat
    18421 tttgcggttc gttattttcc ggtttaggta cgaagtcatc tttgatctga tgaaattcgc
    18481 tatccagcat taattcacta tctttactta agagagggtc tttactaatt ctatcctgaa
    18541 aactttgcag ctctacctta aactgaatca ggaactggcg tttttgttct ttcagctgta
    18601 gatattgatg ttccaaaaca cttaagtact gcttagcttc ctggatagct cttttcgctt
    18661 tcaggtctgc ctctttaata atattttctg cttctttacg cgcattggca ataatatctg
    18721 ctttcgtttt ttctgcaaaa acaagtgttc tactaatcaa ttcttcccgt ctttttaatt
    18781 cttcaactgc acttccagcc tgaacagctt cttgtttaac ttcattttgt atctgttctt
    18841 gacgcgcaag ttctttttgc agttgctttt ggtatgtttc tacatctgaa gctacttgat
    18901 ctaaaaaagc acggacttct tttttactat agccaaacat cgaagaggaa aactcttgat
    18961 gtcttatatc caatgggaat aggggcataa attcctccac ctttatatga gcgagcttat
    19021 cagctttgtt aataaattaa gaataaaaaa agctattatg ggagaaaaat ccaaacccat
    19081 cataggcggt aaaattcgac ggatcggacc taaaagtggt tctgttatag ttaataaaaa
    19141 atgatacaga cggttatacg gattcctgat tatccaggag gcaaaaactc tggctaaaat
    19201 caatatgtta tatatctgta taatacggac aacaaataat aagatggaac ctgttaaact
    19261 cgttgtataa atcaatctaa aacctcatga cagttcctac aacgcgcttc gtatgcatct
    19321 gtggaaccca ctaaaatttg ttcatcctgg tggacagttc tttgggtccg attagcagga
    19381 ttaccgcaaa ttacacaaat agccaaattt tttgtaatat attctgcaat agccattagc
    19441 tgaggcatac taccaaaagg ttcaccttta taatcttgat ccaaaccagc tacaataact
    19501 ctatatcctc tgtctgctaa atcattacaa ataccgacaa tggaggaatc aaaaaattga
    19561 gcttcatcaa tggcaatgat ctttacatca tcatctaaat agctgtaaat ttctccagga
    19621 gaatttatcg gaatggaagc tgcttgcatt tgtgaatgag aaacaatgtt attagcatca
    19681 taacgattat caataattgg cttaaaaacc tgcacctttt gcttagcata ttcagcacgc
    19741 cgaattcttc gaatcaattc ctctgttttt ccactaaaca tacttccgca gataacttct
    19801 atccatcctg ttttctggtt tactatattc agcattcttt ttcctcccca aaaaaactac
    19861 aattaccata tcctatatat cccttttttt gtcaattgct catttttctg atattgtata
    19921 ggctaaaata gcagcagaga tagatacatt taaagattcc atttctccga gcattggaat
    19981 gcaaagtgtt tctgttgcaa tctccaaaat agaagaagaa agtcctttag tttcattacc
    20041 caaggcaaca atatatggtt ctgaaggttg ttcaaaattt gccagtggga ttttcccttg
    20101 catatctaaa gcaaagatgt tagcattcaa ttctttcaag ttctccgctg cacagttaaa
    20161 aaaaggaact ttataaacag cacccaagga agcgcggatt acttttggag agcttacttc
    20221 acaggtattt ggagatagaa tcaagcaatc aatattaaaa gcagcagcta ttctaaaaat
    20281 tgttcccaaa tttcctggat cgcttattcc atcaagataa aatgccttac gaaaggcaat
    20341 tttccttgca acaggtaaag gaaacaaacc ggctattttt tggggatgct cagaatcgca
    20401 tatccttaga agaccctgtt ccgaaatagt atatattttt tcggctgtta tagtaggttc
    20461 ttcattagtc acatataatt ctattgcttt tatgccccaa gagaagagtt gttccaaagt
    20521 ccttttccct tctattatta ccagtttttc ttgcaggcga tatttcttct gtttcagttt
    20581 agaaaggtaa cgcaaatgtt cagcgctaaa tgaaggatat gcaatattat ttatcatcaa
    20641 ctacctcttt taagcaatat gctaaattat cagtcagata ggaacttatc cacaagccct
    20701 gtggataact cttaaattct aacttattga tatataaaga ggatgggaac tgttgagaga
    20761 tttccacaaa acttatccac aggttatcca cattctctcc aagcccataa tattagtaca
    20821 agatagtcat tttttttgtt aattttctct ttccgagctg taattttaaa aaataaatcc
    20881 cagaaggtaa agctctaaaa ttctgatcag aagagaggag gaaatttcct tctccgggat
    20941 aaacttctcc caaatactta ctaagcactt tttggccttt caggttatag atttccagct
    21001 gaatttttcc tgaaagttca tcacctgccc accatcttag aacagctaac gaggttccgg
    21061 atataggatt ggggttaacc aacatcgaaa catttggtgt agaataatca taagaaccaa
    21121 ttacgggttc acttttattt ccattttggt caattgctct caattgataa gttccagatt
    21181 tagaaatttg cagcattaca ttaaaaatgg agttatcggg attaggataa atatattgaa
    21241 aatgttcaaa tccatcgtaa cttatttcat agtaaagaga atcgggatcg gggcttgcca
    21301 gaaaagagag ctgtagagta ttatagatca aattctggga atttaggata ggaggttggg
    21361 gtgggatatt atcattacca agagcagcat ttacaaaact aagccaacga gttttggcaa
    21421 aatccaaatt aaaatagcgt tgccagccgt tttcaaaatt gaatctgctt tctggaaacc
    21481 agaaagctgc cgttcctata tttcctgtat aatctggatt agtaaaactg ctgctaatta
    21541 ccattgtatt ccaaaggtta agtaaatttt gggcttcgct atcacaaatc gtttgtgcct
    21601 tttccaaaaa gtccctgata tcgatatctg ccaaccctgt attcattgaa tagcaggaat
    21661 gacgaattgg caaatattta gaagcaagag gtctttggct tatggcaaaa ctcttaaatt
    21721 tttgcacaaa caaaggcagc atttctgtct ttatcacaga gcaagttatt ggccaaagat
    21781 ttcctccggg atttatacct tgaccgggca gataggaaga aacatagata tcgggaattt
    21841 gagctataag aatatctaaa tctccttcca agagaggaat gatcttttcg taaggaaaac
    21901 cgtattgcgg gacaagatct tccgagccaa taatataatt tgcacaaaaa cctatttcat
    21961 tgataacttc aatgctctgc atactacagg catcaaacag taaaacatca agtttcggga
    22021 tatctgttaa ggcaagagta agttcacctt cagcaacact gatcaaattt tgagagcttt
    22081 catcagggca aatccatttt ggatcatttc ctttatacca actatctcca tgtgaccaaa
    22141 ttatcagcat ttttcgttga gaagggaaat tgttgaaacc ccagtttata aaactattca
    22201 agacctctgg attggcactg tctataattc ccaaattagc caaaactggt gaagaaatat
    22261 cttctgaatt atcctgctgt attctaaagc gttttgctcc ttctggaaaa tcagcttgga
    22321 caataagatt taagccctca ggttgaggaa cgctttccat tgaattaata tccagccgtc
    22381 ccatttctgc caaattatta tcggcagcca tatagacgag aaccgtccag ctttctgcat
    22441 taagaacaag accaatagaa agcagcagga taatcagaac atacttcaat agggatattc
    22501 cttagcttct tcgcttccat ttaaaactct tattccacct tggcagagtg cttccatttc
    22561 tttttcacca gggtaaacat agaggggagc gataaactca gtatgttgtt tgataaattc
    22621 tacgatataa tcgttatatg ctaaaccacc actaagatag ataccatcta cttttccgga
    22681 tagaactgct gcgcaacaac caatttcttt ggaaacctta tagcacattg catctaaaac
    22741 attttttgct ttttgatcac cttcactaac tctttgggca acttcaatac cgctatcggt
    22801 tcctaaataa gccataagtc ctgcaccctt agagagataa gcagtaagtt cttttttgga
    22861 gtattgtcct gaatagcaca tatcaattaa atctccgatg ggaagtgagc cagctctttg
    22921 aggagaaaac ggtcctgttc ctaaaagagc attgtttaca tcaacaactc ttcctttttt
    22981 gatggctgca acggaaattc ctccgcctaa atgaactccg ataagattta catcttccag
    23041 cttttttcct aattctgcag caagtaatct acagatatag cgaatgttta aagcgtgaaa
    23101 taaactttta tgatcaattt caggaatacc tgataagcga gcaatatctt ccatttcatc
    23161 agttgctaca ggatcaacaa taaagtatgg aataccaaga ggttcggcaa ttgccttagc
    23221 aataaatgca cctaaattgg aagcatgcac tcttccccaa attgttgaat cttgcaaatc
    23281 tcgtaacatt gaatcattaa ctttccaggt tccaccgctt acaggacgca ccaatccacc
    23341 tctacctaca acagcattta aggtttggga gtggacatta ttttcttcca tcgcttccat
    23401 aaccagggtt ttacgaaaat ctgtctgatt aaaaagatct ccgtatttgt tcaagtcttc
    23461 aggatcgtgc cttaaagttt tttcaaaaac aggggtgctg tcatcataaa cagcaatttt
    23521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn taaacaagga gacctaaatg
    23641 tcagatacag agaaaaccaa atgtaatcac aatggtgatc atatttgtga tgaagaatgc
    23701 gaatgcaatg acaattttat cactttagag atggatgatg gcactctgaa aaactttttc
    23761 atcatcggga atctggaaaa tgaaggtaaa tattatattg ccttagtagc agaaggtgaa
    23821 tccaattacg acatccttca agtgaccgaa gaaggcgata atgtgaattt gaactatatt
    23881 gatgatgaca atgaatataa caccatcgcc gataaattcg atgaattgtt tcaaaaagaa
    23941 ctaaaagaaa tggggattga ggaagagaca gaataattag ctacggcata gttatactgt
    24001 attttacaag atcttaacta tctaaagaac agttctattc caaaatttta tcttacaaca
    24061 gaaggggtag cgatatttgc tatccctttt tcatagaagg gaaaagagaa gccaaactaa
    24121 tcgtatatta taattaaatg acgcttttta gcaaggagga gcaactatat attatcaatg
    24181 aactactttg gttggtttat aaaaacaaga gcaaaaaaac atttctggta acagcaagtg
    24241 aaagaagtta tcgagaatca aacttctctt ttaaaatacc tattttattg ctaaaaaatg
    24301 tcttaaaata agttaacgat tccttcaata gtccgctttt ttatgaagaa cattttttgg
    24361 gaaaaaaggc taatttcgct atgtgttatt actgattcat taatcaaatg aggtgttgta
    24421 aattatcaaa ttcgggactt ctgatttctg ttgtaaaata tagatattct ttgacagatt
    24481 tccctatttc atttttgctt acttagataa aaaaagggag tgagagatgc cttttgtaca
    24541 tttacataat catacccaat atagtatgtt ggatggagcc tgcagggttg acaggatgat
    24601 aaaactggca aaaaaatatg aaatgcctgc agtggcaatt acagatcacg ggaatttatt
    24661 cggtgttgtg gatttttata aaactgcccg tgctgcaggg attaaaccta taattggcat
    24721 tgaagcatat atagtaaatg gcgatattga aagtgaaagc agcaagaatg aaagtcgcta
    24781 tcatataatt ttgctggcac aaaatgagca gggctacaaa aacctagtaa aactctcttc
    24841 tatttcctat ataaaaggat tttattataa accgagaatt tccaaaactc ttttgcagca
    24901 atacaatgaa gggcttatat gtctttctgc ttgtattaaa ggtgaaattc cttcgctaat
    24961 tttgaatgaa cggataaatc aggcacgcga agttgcttta tggtataaag acctctttaa
    25021 agaccgtttt tttatggaac ttcaagatca caatctggaa cctgaaaaag ctgttatgcc
    25081 ccgtttaatt gatctggcaa aagaattgga tataccgatg gttcttacta atgattgcca
    25141 ttacctgcat caaagtgatt atgaagcaca tgatatcttg ctgtgtattc aaaccggaaa
    25201 actgttaaat gatcctggac gtttacatta tagtactaat cagttatatt ttaaaagccc
    25261 ggaagaaatg aaagaactct ttccggaagt tcctgaagca tacgctaata ctttaaaaat
    25321 agcggagatg atagatctaa cattgcatta tgataaattt ctgctgccta atatagaaac
    25381 ccctcccgaa tttaaaacaa tgggtgaata tttaaagcat ctctgttatg aaggtgccaa
    25441 aattaaatat cccgagatga atgaaaccat taaacagcgt attgattatg agcttgaagt
    25501 gatcgataga atgggcttta gtggctattt tttagttgta aaagatatca ttgataatgc
    25561 ccgaaaacaa aatgtacctg tgggtccagg tcgtggttcg gctgccggaa gtataattgc
    25621 ttatcttctt aatataactt tagttgatcc tataaaatac ggactggttt ttgaaagatt
    25681 ccttaatcct gatagaatca gtatgccgga tatagatatt gatttttgtg ctcagaagcg
    25741 gggacaagtt attgactata ttgtgcaacg ctatggaaga gagagcgtta cgcagattat
    25801 aactttctct actttaggag caaaaagtgt gataaaagat gtagcccgag ttttaagtgt
    25861 gcctgcttct gaggcaaatg ccatcactaa aaccattccc ggtaatgtga aaagtttgga
    25921 tgatgcctat aaacaatatc cggaatttgc tactcttatt gatagtaacg atttatatag
    25981 aagtatattt aaacattcaa aagtgttgga aggtctggtt cgtcaaacag gagtgcatgc
    26041 tgcaggagtt gttattgctc ctggagatct tacagattat gttcctcttg cttgtagttc
    26101 gcaaaaagat gaggaaaaag ttattctggt tcagtatgaa ggaaaatggc taaatgatct
    26161 aaaactacta aaaatggaca ttttaggttt gaagaacctt acggtaatac aaaaaactat
    26221 agacctggta aaacaatctc aaaatataga tatagatata aatctcatat ctcttgatga
    26281 taaaaaggtc tatacccttt taggtaaagg cgaaacggat ggtgtttttc aatttgaatc
    26341 ggatggtatg cgtaaatacc ttatggaact gaaacccaat aaaatagagg atttaattgc
    26401 tatggtagct ctctacagac ctggtcctat gcagtttata gatagttaca tagctcgtaa
    26461 acatggacgc gaaaaagtgg tttttgatca tccgttgatg gaaaataccc tcaaagagac
    26521 ctatggcgta actgtctacc aggaacaagt tatgcaaatt gctcgtgaaa tgggtggctt
    26581 tacaggtggc gaagcagaca gcttgagaaa agcaatcggc aaaaaaagtt ccgagttaat
    26641 gcagaagtac tatgaaaaat ttatccaagg cgctaaaaca aataatgttc ctgaaactgt
    26701 gagcgaaaaa atatggcaag attggttaca ctttgctgaa tatgctttta ataaatctca
    26761 tgctacttgc tatgcattaa tcgcttatca aactgcctat ctgaaaacat actatcctgt
    26821 agaatatatg gctgcgttac tatctctgga agatgatcct tcaggaattc ccggaaaaat
    26881 tgaggtgtgt aaaaagatgg ggataaaaat tatccctccc aatattaatc gcagtgatag
    26941 tgaatttcgg gtgcataata aagaagtcca atttggttta agagctataa aaaatttggg
    27001 ggaagcagct atgcgagcta ttatagaaag tagagatcaa gggggacctt atacaggtat
    27061 cttcaatttt tgcagtcgct tagatagcag cagcgtaaat aaaaccattc tggagagttt
    27121 aattgcttcc ggagcgatgg atgaactgga aggaacaaga gctcaaaaat gggcagtaat
    27181 tgaacaggca ttacagttta gcagtaatga acaaagagat agaaaaaagg gacagagcac
    27241 tatatttgat tttttggatg aggaagatga taacagtaac tattatcctc ctctgcctaa
    27301 tgttgaacct tggggctatt tatatcagct ggaaaaagaa aaagaggttt taggtttcta
    27361 tttgagcggt catcctttgt atgaatacag gtctttgatt aaacatataa ctaatgctga
    27421 ttccaatact ggcaaaagca acaataacgg agaactatat cttgcaggaa ttattactaa
    27481 tatctccaaa aagaaagata gtcggggcaa tcaaattgcc ttcgtggaat ttgaagacct
    27541 tgccggtaaa tttgaagtaa gcttgtttaa taaggattat ttgcaatatt caaagataat
    27601 tagcccggga aaggtctttt ttataacggg cactaaatcc acttttaatg gtaatgatga
    27661 tgctatgctg agaattttgc ccaattctct aattcccttt gatgctttag ccaatgaact
    27721 aagaggggaa ataaaagtac acatcaatga caaacaagtg aaaaagggtt gcttaaccga
    27781 tttgggagaa agaatatctc gctgcgatgg taaatttaaa ctgattacta ctattcagaa
    27841 agaagatgtg aatagttatc aactggaatc ccgtaaatta ttctttcccg gggaaggact
    27901 tttaaactgg ctggaaaaag aaaaaattga ttttcaaata agattggtta caaatggaaa
    27961 aagtacttag tatcctgatt ctactattta cctttggctt tgtatctgct caggaaattt
    28021 tctatgagca ttatgcaaac caggttgata tccgtgttgt tttgccttac aataatatta
    28081 gcttcaaaaa aggttcggac caagcagtat accaaattgt gctcgagatt tctaattccc
    28141 ggaaaaaaca ggttgctacc ttggaaaaaa gccaaattat accaaaatat gaatggctca
    28201 ctgattgtgg cattccagtt ttctttcagt tggaacttcc ccaaggttca tataatgtag
    28261 ctttgaaaat aaaaaataaa gcgatggggg ataaaagaaa ttatactcgc caatttatca
    28321 taggtccttc cagtacggaa attgggctta gttgggttat tgctaaaaag gatggtattg
    28381 attttatccc ttatagttta tccggattga gtgtagatga actatgccta aatcagagct
    28441 attctttggc tttggatagc ttgcgtatcc aaattgataa tcagaactta actataacaa
    28501 atccccaaag cccagtaatt attgatttac ttccttacat ccaaaaagat gcaccgaatc
    28561 aattggcttt aacttttttt gaaaacaata tctactacaa gatggaacct tttctatttt
    28621 ccccttggta tgcatattcg ctgcgctatt ctttgaatga tcaaataaga caaattcgct
    28681 atatagccaa tcaaaacgaa tggaatgttt tacggaatgt tcctgaagaa agacaggctg
    28741 aagtaattga ggcattttgg aaagccaatg atcccagccc tggaactatc agaaatgaat
    28801 tgcgggaaag attctatcag agggttttag cagcagatga actttttaca gtgcataaaa
    28861 aaattgcagg atggacgact gatcagggta gaatatatat aaaatatgga gaacccgatg
    28921 atgtctattc agaagttttc tctataggga aatatccttt tataatctgg tattactatg
    28981 acaaaaacct cgaatttatc tttgctgaca ttggaggtta tggtcaatat actttaagga
    29041 ataaagatga agaatattag aattctaatt tgcttggcac ttctcagctg ttttcttatt
    29101 ccgctatcgg gatcagaaaa attgaatatg tttattgatt attccaggtt cctggataaa
    29161 aataaaaata ctatattact gctcgattat caggtaccct atcagaatct ggcttttctt
    29221 gctaaaaaca atggattctt tgccgaactg gaagtatata ttaaaatctg tgaaggtgat
    29281 agtttgttgc tagaccataa aattaatgac ctcattggag ttcgcaataa agctgatact
    29341 tctaataaac aaaaatcata tctcaatcgc ttcagtttta ttatggatag acccatctgc
    29401 actgtccaat tctatgctca agacctaaat tccgaaaaaa gttttatgca cgaatttgag
    29461 attaccgctt tgcctccaaa cagtttatta agcgatattg aactcaattc tgaagtacga
    29521 gctgatacta cttcctactt gcccaaattt aaaagaaata atatcctata tcgtagcgag
    29581 ccctccattc tctttaataa aactaatacc gattatgttt atctttacct ggaaagctat
    29641 attggaagta cttctttgga aagctgcctc ctgaatttat ccctggaaaa ggattctgta
    29701 atagtgatgg atgaagattt gaattataaa cctgcctcca gcagcgaaag tattaattta
    29761 aaaataccta tttctgacct taacccaggt ttatatctgg ggactgttat cttgcaaaat
    29821 cctgatcaat acgaagaaag aaaattcgaa tttgccgtga tcgaagaaaa agaggaagaa
    29881 tttttcctct ttgccaatcc tgatgacgaa tttgaactta tgcggatatt tatgggaaac
    29941 agaatttctg cagattggaa agatatgagc caggatagta aaaagcggta tataactcaa
    30001 ttttggagaa atatggcatc caacacaaat cgcagcatac aaagcattat ggatttagtg
    30061 caagaacgaa tcgattattc taataaacat ttcggacatt tcaaaaaagg atggacttca
    30121 gatatgggac gcatatatat aaggaatgga gctcccgatg atattcaaag agaaacaagt
    30181 tctgatgaaa gccgcttcgt tagaaaggat tatcaaatct ggaaatattc tactggaaat
    30241 aaaccagtat atctgtttat cgatattcaa atgaatggaa attataaact catttatgta
    30301 gtaaacgatg atatggaaag ctcaaatcct gactggatgc gctatgtcgg ctacgatttt
    30361 gatactagca aattggataa ttaattgtcc aacagcggtg gaaaggcatc ttcgtaatga
    30421 ataaccttga atgtatccgt agattcataa taaaatacta agataacatc aaaacgaaaa
    30481 atatagttgc caaagtttgg attttcatta atatataatt gtgctgctct tgttatcttc
    30541 ctttgtttgg aaaaggaaac agaagacaga gcagactcga tagagtgaga agtacgggtt
    30601 ttaacttcgc agaatattag gtgttgatct ttttccacaa taagatctat ttccccacag
    30661 cgcttacgga aattacggca aacaactgaa taaccgttag ttactaaata acgcgctgca
    30721 agatcttcgc caatacgaga aaaattctga agtgaatatt taatcatatc tcaagaaaaa
    30781 acttgacgga tatttcgtca atgagaatga tggacaacat aagtaattaa caccgaaagg
    30841 tggagggaaa catgaaaaaa attattttga tggcgattgc ggtagttgct gtgattagta
    30901 gtgttcaagc agcgcccttt caaactttag gaatgttgag aactccagat gcctatgttc
    30961 ttcccaataa ggcagcagaa tttgtattag ttggttatta cagagatatt gccgccccca
    31021 aaccacttac taacgatgct aaagacgcct ccgaaggttt atttccttat acaatgattg
    31081 gagtaggtat tttggataga gttgaacttg gtttatttct gggtgattac acttccttag
    31141 acggattggt ttattatctt aatgcaaaag taaaaattat tgaggagacc ttacgaattc
    31201 cccaggtctc tataggaatg gataatattt tatcccctgt tcctaaacat ggctctcaat
    31261 acttaagccc tggtgacgat tttagtcaac atcctgatgc tgattgctac gagccctatt
    31321 ctccttatgc ggtagcatcc aagcaagctg tatttttagg aataccctgg atgttcaatg
    31381 taggaattgg caccaaccgt tttgttggcc aagctaaatc ccgctattta aatggactat
    31441 ttgcttcagc agaaatgtct ccaattaaag atctggcatt acaaggagaa tttgatggtg
    31501 aagatctcaa tattggggcc aaatattcct ataagaattt tggctttaaa gttggtgtaa
    31561 atgctcttga ggattggttc aaggatagcg attacaagga taatatacgg attgcttttg
    31621 gtgtttcata tctgttcgat aagtatgcag aagccaaacg ccgtcccgac cttggtcatt
    31681 atgctggtta cggaaaaaca ccctctatgg gggaaggggt agttgaaacc ccaatcgaaa
    31741 ctggaattac agttcctcca acagttagcc caagtaaacc acaagctgga actcaaactc
    31801 ctgcccctgg tttaaccaca tctggaactg cgggttacga acaactttct cctgaagttc
    31861 aggaattgat gaaagaacta cggatgctga aggaagatag ggaaaaggct cagcaagcga
    31921 tggatgaact gcgcaaatgg attgaagaac tgaagaaaca aaaacagtaa ttctgcttaa
    31981 tctgctccta cagaatggac aggttgaggc attatgtttc ccataaagaa ataaaaaagt
    32041 tcaggattac tttgctgaag cagagaatcc tgaactttgg cccctaatgg aaagagtaag
    32101 gaaggaaaat taagtgagca gcattatgaa aaaaacacta aagattatat tcctcaccgt
    32161 tgttgctttg tcgtttgtta tagtagtctt tgcgcagaat agcaatgagg ctactaaaaa
    32221 agaaattatc cagcaaatat ctcaggaacg acaaatatta aaagaaatag ccaaaaattt
    32281 tctgattgat gttttacctg aattggtcaa aaaagacccg gcactggcag aacaagcatt
    32341 agtgaaatac aaagatgcat actccagttt aggacaaaac gatattgttt atttacttgg
    32401 tcatttctat acgcgtatgg gggaaaatac gcgagctatt agttatttca actctttgct
    32461 ccaaacaaac ctgaacgaag attctcggaa aatgcttaat ttggctttat atcaacaaat
    32521 ggtgcattat ctggaaactg ggaaccgtaa agctgccagg gatttcttac aggtcattat
    32581 ttttgatcaa tatcagtttg atacatatta tcctacatat ctatatgttt gggctgatat
    32641 gactgccgaa gaaggccaat atgaaactgt gcaaaatact ctacaaagct atatgcagaa
    32701 tagagacatt ataatgaatc gccttcttcc tcaaaaaata gctatattag caagagttca
    32761 aaacttagat ttagattcct actatgcaaa tccaagcggg gaaacatata agcaactggc
    32821 ttcccaaata gatgttatca aaggggattt aactaataac tataatgaat tgatgtcctt
    32881 aaaagggatt atatatgtag atgcgattgt aaatctgcat aatgaagaaa ttggtatgct
    32941 tgattctttg aaaattgttt taagtgacta ttataccaag aaaatatatg cagaagagac
    33001 aataacgagg ggaacaagtt tattacaaca aattaaaaaa ctatcatcca gttatgagag
    33061 acagcttgct ataatggacc aaattctgga aagacaatat gagctataca aggcaaatga
    33121 tccccagatt caaggtaata aatatagtga tatggagctg aaacgtctat acgatttaga
    33181 gaagaacatt cagctttata ccgatctaat tgaagaattg gataattata tagccgatcc
    33241 taaactggta gatatgcaag cacaactaaa aattattcgt gatgattacg atgagaaacg
    33301 cacgagttta aatcttcgca aagaatacct actccaagtt cgtaaacact caagtgatgc
    33361 tcaagaacaa atattcaata ctatcctgca agagtattat gcactttatc ctgataagaa
    33421 gaatctcgat aaacagataa tggaacttga gcaatttatg aaagaagaaa cccagaacat
    33481 ttttaccgaa gaaatgcgta ataagatcaa aagtaatgtg gctagtcaca ttgcccaaac
    33541 tgctgatagt aataaacgcg atgagcccat tcgcaagaat gctatagact tggtagcgaa
    33601 tgcagaattt atcaagctcc aattggatta tcgtaatctc cattttaaag agcagcaacg
    33661 tttggctcaa aaggaaactc ttactgaaca acagcttgta gaacttcaag cacaaatagt
    33721 agctgaaaaa cgggatttaa taacaagaat tgaaacattt atttccaata atcctaattt
    33781 taaagcggtt gaacaaccca atggcgattt cctggttact aatgctgatt tgtattacaa
    33841 cctggcggaa cttcaatatg cagcttattt ggataaccca agtatcgctt tggagagcta
    33901 tagaaaggtt gttcaaattg atcccaatta tatagacata gatgctgctt tatataacat
    33961 tggctttatt agcagtgaaa ttaaccatca aagaatagat agtaataaga gccgttttta
    34021 cgaacttaac aaaactgccg tagctcttga tgataatagt aaatataaag aatcttactt
    34081 tgcggaagcc attagtgcgt atcaaagcat tataaataat tataaaaatt caccgtatta
    34141 tgaagaggca atctaccgtt tgggtatcct ttattattat attgctacag atgctgatgt
    34201 accgcagcaa tactatgagt tggcaacgaa ctattttgat actattatca atatgcccga
    34261 tagtaagtat aaatatgatg ctatttacca gcgtggttgg ctccgtctga attcatacca
    34321 ggaagctgaa ctcaaactgg caatgaacga ttttataacc ttgttaaatg ctgtggataa
    34381 cggcaaaata tccgatcctg caatagcaga agattatcgg aacgatgcaa ttgataatat
    34441 tgcctattgt ttgatcgctc tggatggaac tgatttctat tctcaagcta agggagtaac
    34501 ggagctgcaa aatatcttta gtggttatca aaatacagaa gtaatgaacc gcattgtaga
    34561 taaggcagcc tccaataaat ttgatcttgc tgcttctttg caagcaattg acttcctgtg
    34621 gcttaagatc aatatgaatc ctatggctct tgaaaatcct accttagtag attctatcct
    34681 gaaaatatat gccagtgcgc aatatcagct acgcgaagat caaaattttg atcagatagc
    34741 tcatgagctc taccaaaata taattactaa ctatggtaag aactctactt ggtatgcaac
    34801 gaacaaagat aaaaatatct cagcgcagct ggcagtgatt caaaatgctt atgagaaaag
    34861 agcactgcgt ctgcataacg aatttcttgc agaaccaaca aatgaagctc gcttgcttgc
    34921 ctactatcaa catatggatc aatatagcaa ttttatggaa acgcatagcg agaactatcc
    34981 agcttggaaa gcaaaaacag ataagaacaa actattatta tctataactc ttgccgaacg
    35041 ctctaattta cccaagcact atctttctgc tattaaatgc ttgcaggact ttaatactaa
    35101 ttatcctcaa gatgaggatt tcttcctaaa cgaaggtctg atttatgtgt atactaataa
    35161 tatatataat cttatgaaag acctctacaa tgaaaattat aatccagaac ctggcttgcc
    35221 tgcaaatctt gatgaactgt ttgctctatt aagtaccaat tcaatgcgtt tcattaatgt
    35281 tttaagaagc gatgcttata aaaaccctga acgcgaaagc caagcagtta ctatcttatt
    35341 aaacttggca gatattcaat ataatagagg taagatgcca gaagcaaaag agttgtatct
    35401 tacggctttg caaaatgaaa cgcttatctc caataaaact aaatttgata tatacggcaa
    35461 actggcatta atttctgaac aagagaaaaa ctttgctcaa gctgaaacat attaccgtaa
    35521 agctttacct tttgcttcta cccctactga aagagaagct attgcaaaca atataagata
    35581 ccaaattcag aatagttatg aagctgctga acaagcaggt aactattctt ttgcggctag
    35641 tgagcggttg cgtttagctg cagaacttga tccaaaagat actcagcgta ttcagggata
    35701 taaaatggct gctcaagaaa catatattaa agccaaagag tatcagaaag ccatagatat
    35761 tttattggaa ttagcaggaa caaaaactga tatagacgaa atttactatt attacttccg
    35821 cgctgcagaa atagctgaag cagatacggc tatgaataat ccggaaatag ctaaaaacat
    35881 caaagacagc tttatagcta aatatcccag tagcaatcag gcatttttat tgcgaattgc
    35941 tatgcttcaa gagatagaga aagatcctgc acaaaaaaat gcagcagctg aggcttatct
    36001 tgcgttgcat gaagaagcca gaaaaaaaac catcaatact ggtgatatca ctccagatgc
    36061 cttattgtta aaagccagta ttaactatcg tgaagccgga aataaggaaa aggaaattga
    36121 aatctacaat caatttctac agctctatcc aaacaatgaa aatgtaattc cctttatgca
    36181 ggttattgct gatgactatc tggccaaggg agatacgctg aagtttgagc agttagcaaa
    36241 acaaatatat actaaggata aaactaaagg cgatcgctat cagtggattg ccactactaa
    36301 gttgaacaaa ttaatgtatg attttgataa tgcctacaag aataaaaact attctgaagc
    36361 atttaaatac cgcgatgaat acaaacggct ggaagctgct tacaaaaaag aagggcttca
    36421 atttgaaact gcagatttta gctccgctaa gaactatgag tattttacat cagtggaaaa
    36481 tgaatacaag gcaattgaaa agcggaaagc gttcttaaag aacttcgatg ctcaacttac
    36541 tgctattgag aaaggcaact taatgacttc ctcccctgct aagctaatca aggttaatgt
    36601 aaatactacc tggcagaacc atttaattgg tggttcctac aggcgtattc ccatatttaa
    36661 ggaatcggtt agagcagaag tagccaaggt gaacaaggtt cttagttcag ccgagaaaga
    36721 aaatatcgag ttagaaaact atcgtcggct gcgtgcgtta aatctaatag ctcgtattta
    36781 cgaaaaaggt gtgaatgtta taaacaccca aattggtgtt tatgttcgca cttcctatga
    36841 ggcaacgggt gtgcgtcagc aatataaaga tatattggat tcttttatta attctgttgc
    36901 tgctgatcaa agcaatgatc tgctcagttt ggcatataca actcacttga acatatataa
    36961 ttattatcag atggctggtt atacagatta ttacactcaa caaagctatg ccaaattaca
    37021 gcaatggaat ctgctacccg attataagat agatgaatat ccgcttggca gtggctggag
    37081 ccaaaaaatg gatggtaata atattaattt acccgctcaa aatgtaactt cacctaaagg
    37141 tggtcgtttg ggcagtattc agattcctgc agggaaagat attattctca gccatactgt
    37201 taatgctaaa ttggtacccg attttgccct aatccaacta gtttatccgt atgatgtaaa
    37261 tattagaatg aatggtacag atgtagaggc aggggttgtt cctattgaca ctttggatgt
    37321 taataaaccg ggaactacta ctcgttatgc atatttattg ccttccagtt cttggattga
    37381 agggcaaaat gcagtggaat tgactgtccc taataatact ccagagcccc agaatctgta
    37441 tttatcgttg caagtattta cagaccgcca gaaaatagct gcttccatcc ctccggaaac
    37501 ggttatgcta tatacagacc ccagctggag gatagtaaat gtgaatcagg aaactggaac
    37561 tgaaacattt agtgcagcta caataaccgg taattttggg atcagtttgg ataacattga
    37621 agggattgag ggtgaacaag ctaaaccgat ttggcctgtg gaagaagcgc caattagtag
    37681 tgcagttttt gaagtggatt tctatctgga tactgaattc agagaaggtc tgatcaattt
    37741 tgtggcacct gaaactgcaa ctgtgtatct taacggagag ttattagctt ccaatcttgc
    37801 tatggattac gatccggaac cgttccgcgt atatactact caggtttcaa tcgaacctgc
    37861 caaagtaact tcagggaaaa ataccctgcg ttttgtagtg cagaacaatt ccaactatcg
    37921 tggctttata gctacagtaa aaatcatcaa agctggaaag gaggaggtac gatgaagaaa
    37981 agtttcttac tactggcagc gatgctcttt ttggcataca tcgggtatgc ccaaacggtt
    38041 actccacaac ctactaatca acagcaaccg aaaggttttg aaggtgttgg gcagtatgta
    38101 gaaaaagaag aattaataat tgaagtgaaa tccttaattc ctcctacggg tgtttttcag
    38161 gataaatccg atacatatca gaaatcagga gaatacaaat tttccttttc tttagaagat
    38221 attcgaaaac aaagcgatat attactaaat aaatggctga aggagtcaaa atgaaagcaa
    38281 aacatatcgt cctagtgatt atctccctgt ttatttgtgt tggtctattt ggccagaatg
    38341 caggagaagc cgcagctccg gctgcaccag ctagtcaagg ttacagtata gttcaagtat
    38401 ttgttgatag cggcaattgg gcttacgcaa ttctgcttgt ctttattatt ggtcttgttt
    38461 atgccattgt ccgttggaat caactctacc gtaaagaaaa aatcgatgca ggtggattct
    38521 acttgaagct tcgtaattat attagaaatg accagctcga ggaagcagcc aaaattgctg
    38581 aaagctataa gggcacaacg atgggcttta tcttctggag cgctttgatg atttttaaag
    38641 atgccaagaa gagtggacaa tcgggtaatg atttaagaat ggctgttcag aattcggtag
    38701 aagaatctgt attacaaaca gttcataaat tggatagcgg attattttgg tttgacacct
    38761 tagctcaggt tgctacttat cttggacttt taggaaccat ttggggctta ctggaagcat
    38821 ttgccagctt atctggttta actggtgccg cacaacagac agctctaaca gatggtatta
    38881 aaaaagcaat cggaacaacg gcattaggtt tgctggcagc tattccttta actctaatta
    38941 aaggttggct gttaacccgt gccaataatg taatcagtga tatcgacgaa tatagtgtaa
    39001 aattgatcaa tactatcaac aacgccatta aggattaaaa ctatggaacg ttacagacca
    39061 tccgcaatgc gccagcaaaa gtcgcaaacg atacctcagg aaccaaatct ggtaccgata
    39121 atgaatctgt ttttaaccat cattccaatg ttaatgatga tggtggttat ttctcagatt
    39181 gcacttttgg cgctaaattt ctcgcaaggg gaaggcggtg taactactgg tgggggcgga
    39241 ggtggaggtg gtcctgtcac cgaaaaagtt gaaatccata tcttagggaa gaattatgcc
    39301 gcaagcacaa atttcctagg tatggaaatt cgcgaaccag gtgtagcaac caaaaacatc
    39361 ccagccatca atggatttta tgattatcca tcccttgata aagctctcca ggagattaga
    39421 agccgcaagg gtaatttaaa cgatatcacg gtcctggttc atcctgatgt cctatatggg
    39481 gatttgatga agacgataga tctgtgcaaa gtacatgggt tttcccaggt tcattatagt
    39541 gcacccaaag tgcagtatta ttaagggagt taattatgca tattactgat aaactttcat
    39601 cttccaataa atctgcacgc cgcgttagaa gaaaatttga agaggccaaa agtctgtcta
    39661 ttacctcact tttagatgaa ctcacaatta ttctggtatt tctagtgaaa aatatatcca
    39721 cggaagcagt gaaaatatct tctgatcctg ccatacaata ccccactact atcacgaatg
    39781 atgaactttt ggcaaaagga gttgccactc ctattaaaat tttcccagac cgtattgttg
    39841 taggaaccga agcactggaa tatggttctc cagctgattt actggaaaat cctaagaaac
    39901 ggcaagacct aataacttat ttatctgcag aagtaaactc tattttacaa caaaaagatg
    39961 cggaaacttg tcttcttgtt caggccgatt attccattcc ttgtgattat atcaccgaaa
    40021 ttgtccgcgc tggtactgct gctggatatt cttacatata ttttgccacg cttgaggatg
    40081 ctgactggtt acaaaaatat aatcttagct tagctcagca ataaggaggt atgatgtcaa
    40141 aattattaaa cttaactctt cactataaag gtaaagttct tgatcgggtt cgttattgtc
    40201 gtgatttcaa gaacaagttc tttataggtt cagataaata cctcttctgg caaattttag
    40261 attcatcttt tccagagaag cacttatttg taaccaaaaa gggggatcag ctttatttgc
    40321 aacttcctcc tggatcaaaa gtaagctgta ctaagggcgg acaaccagta gatagtgctt
    40381 acctcacaaa gaataatata ctgaaggata accggctatt attattaccg gatatgactg
    40441 gcaccttaaa tattgcaccc aattgggaga tttcttacga gttcaaagaa ccttttgtaa
    40501 ctgttcttac tcctgaagag aaacaaattg cttcacaata tgcccgacgt ccccagccta
    40561 catctacaga aaggtttaac cgcaatatga tattgctttt tattgtgctg accattattt
    40621 tcgtaataat cttcgaccaa tttctaaaaa agcaaataag ctatgattac acactggaac
    40681 agaaagtagc gatgttgcaa aaagctgaac aaattaaagc agaagcatta gcaaaaccaa
    40741 gcacttttga acctacgggt gaaactcctg aagctgttac cgaaaaagca caacaaggag
    40801 taaccggtgg tgctattgga ggtactggaa ccgccaaatc tgccggtgcc attttcggtg
    40861 gaggttttgg ttcttttgat ccaagtgcta cttctgcagc aagaccaatt caggccgtta
    40921 ctacagctga aagctttgtt acttcccgtc ctggacgagg aggtggtggc ggaggtatag
    40981 gaaccggagg agggggtgca ggacctagtg gaggttattc tactagcttt gatcctaatg
    41041 ctcctagaac tttttccagt gatattggtt ctgtagctac acaagcacct caagttgctg
    41101 gttcaagtat tcgtcctgga gttggaacta tagcaaaagc tacaggagat caaagtcgct
    41161 tagctcctag cggagttgta tttggtcaaa ctgctcaatc ttctcaactg atcagttctt
    41221 tccaatctaa aaaagtagct caggtttcgg aagggactat tgcacaagca cctgcagaaa
    41281 caaaaaccag atatgaaacc atcggggctt tagttaattc gcggagaagt cagataaata
    41341 ctctatactc aaagtggaat gcgataattc ctttttcggg gagtgttact atccgtctat
    41401 tgataagttc ttccgggaaa gttcaggaag ctcttattac acctaatggc cagataccta
    41461 atggattctt aactgagctt aaacaactat gtgaatcgtg gaagtttcct gttaccgaag
    41521 aaagtgatta tactttctct gttagattgc gtcaagctta agctctcata acaaaaacat
    41581 aaataagaaa ccttgctata agaagcaggg tttctttgtt ttatgtccgt tatcgaacga
    41641 taattcgctt gctatttttt ctctttgttc gtgttatcca agattggatg taggagagaa
    41701 gtatatgctt ggttatttgt acaaaaccaa tagggaagag gtttttggta tgagaacagt
    41761 ataactttgt gagcaggaac aagctgaagc ggaatatcta aactttataa ctaattctac
    41821 tcttgagata tcttaattca ctgatagtat aagcctgcat ctctattgct tatagagcgt
    41881 tagtgtct
//
LOCUS       DAOSTH010000010        39500 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_1138_length_39500_cov_11.908683, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000010 DAOSTH010000000
VERSION     DAOSTH010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 39500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39500
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1138_length_39500_cov_11.908683"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(648..980)
                     /locus_tag="PLB65_02965"
     CDS             complement(648..980)
                     /locus_tag="PLB65_02965"
                     /inference="COORDINATES: protein motif:HMM:NF024087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3795 domain-containing protein"
                     /protein_id="HOQ77517.1"
                     /translation="MKKVISACGVDCFKCETYIATQNDDEARKEDIAKRWSEHYQGEI
                     HPEDINCDGCMSEGAKFSWCGLCPIRLCVIEKGYISCAECSDFPCEKNEYVYQVDPSA
                     KENLERIR"
     gene            1286..2107
                     /locus_tag="PLB65_02970"
     CDS             1286..2107
                     /locus_tag="PLB65_02970"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOQ77518.1"
                     /translation="MCNVIIREPVMVVDIGNTNILCAIYLDGEPIWTARFTSSREKTS
                     DEYYILLSGLIESSVLSEEEKIAIEDINYVALGSVVPELTRVWRHLFSKYFKVEITEI
                     NALSPLGISFRVENPSFIGADLIANAYAALHKYKSSSIIIDLGTATTVELISNERRFE
                     GAIIAPGMKLGAEILFQRAAQLYEIELVQPPVLLGLNTTDAMLSGVVYGHSIMLDALV
                     VKIKEQYSQLAPIRTILTGGMASLVKPSMKEIDVVDRHLTLDGFYLAFCRILQQK"
     gene            2114..8578
                     /locus_tag="PLB65_02975"
     CDS             2114..8578
                     /locus_tag="PLB65_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77519.1"
                     /translation="MLKKLFLCLLLIAFTLLWANEPTGEVNTNPEQEETGQKGLFPVK
                     PFRQKREGQHQLDPLTEKAMYPRLYIPDYNPVINNRIEVYKINKAMNYYKARPLEIPA
                     LAPEYKEKIDFTNNKVILSVQVGDYKIAPDVIISFDRYFSNMQMKAFHKSIIANIKTQ
                     AQATQTVSSGLFKDLTLLPEIAMPKAMQKVLGSSAGRLNLDGTQKLTLQASNTRRKQV
                     PIYDESGKNVFDLKMEMETNLRLSGTIGDKIAVNFKYNSKQDEQLFDMNNVNVKYTGY
                     DDEFVQSIEGGNIALSLGGSRYVSYSASSQGLFGITSKFKYGNLDLNLIASKEESQKS
                     TQTYVGKSQADSSTVSSWKYAKRTMYYLHDPYQLYQLKTGSNIPPGWVNNAIDTAPDG
                     SWLVNENYLPVSGTVRLFYDDANYTNNTAAAKGDTIYYSNDVRPPYEPWYDELIEGTD
                     FVTDYDSGIITVLKNIDRTATLGVQYTRRDGVQVPPPNPDTSILHVKVLRKKYQEYIP
                     YNSNDELNTWHLQMRNIYDMGRTNIKNDGFELQVYTVNVDLTRNYNLPDNLVFGEIST
                     YNDYLRLNANNSPDGIINGDDATVNLTNGWIIMPFIEPMQGLGDGIIYRKENEDEYSY
                     QDSTSIFINIKGKIGREAIELSSMGVLKGSVRVRVNGVEQRENTDYIVDYDFGRITFL
                     TAAGKDPDAKIEIDFESRTLFSVAQKNLAGIRANWQMNDYTKLGGTLIYRSESVADKR
                     PRIGNENIEMIMGDIDGEVTIKPEFITRGLDALPLINTTAPSQISMSGEIAFTLPNIY
                     GDPDSKKKEAYLDDMESIMDSYPLGVTYNNWILGSKPFGTSYAKGRINWFNPKDVKRR
                     DIEAEATLTDQEKDEYVTVLTMIVKPNPVQIPNSTTHSWAGIMKYLGNQLDFSQKKYL
                     EVYAKLEPINPNTNIYPNVTMHIDLGDINEDFYTEFGGYGVLNSEDVNRDGVLTISED
                     IGLDGISKNQPGHDPNDIAYPASGNDYSGVNGTEDNRILDTEDLDGNGVLNTLDRYIS
                     YSFSLSNPDTSIVIDSYNQWRMYRIPLSDEQYFQLVNSSGTNIQPSLQKVSYARIWLD
                     TDCTMDIKVRIADISVVGNKWQDNYIRQFKYSSAPPPNSYVQYYNLMIDPTVLAAKNT
                     SFISGIVSNQKNFSHYTSPPGTWYTEENRESAESALTLEVKHLQRDQMCLLRQKMLDT
                     QNLLSYDKIRFWVYPEAAQGSYSHLDSLYVIFRIGADSLNYYQVRQRVPVHPYNNKIS
                     AKDWIQLEYNLQDLISLKEINPNADADSLVNIVPGDEGKIGKYYRGKPSLINIREIYL
                     GVYVPGTHFYAAEEEIKEFSGTIYFDDIRVADPYEDIGIAKRLSLSGTFADVATLNID
                     YEEKSENFNTTIQRGRSNTFTSVRSLNVTNKIFVNKLLPNSWNMDIPISLNRNYSLGI
                     PRFRANSDLLRANMADPELKEIEKTEHLAYSADFGLSQKQPPKSKILQYTIYRTSISG
                     RIENSYNNTSTAIDTLLAYRGTLNYNLNLPADKTSLKLFNDYRLGYFPNTFSNSFTFS
                     SNEPKSWDRVTTVDSLAWNKRSQTTDTRTITTDNNISWNILSDVTATARLNTKRDLKQ
                     KDYIYDINVGKQTEYVQDLGLNYSPNYLPRIFNFTSSASARYTDTQRKYTQYIGGSAV
                     DTYQRDGNTNRSIRMNLTLMNSSLFTEWTTKMMSKMPEGTISPKVKDKDKYPKSEMSE
                     EEIKKQEEHQKEEEIKKQEEHQKEEEIKKQEEHQKEEEIKKQEEHQKEEEIKKEELKP
                     EEEQKLSEEEINKRQDEIARLKAEGKFDQLSEEEINKLLADISAGKGKKETQENDGEK
                     EPEANGPGFNPLLTFVSALSHIKNINASYQNTYVMNYARKTNPFPFAFQLGIPHCVPY
                     DSLEAISNDNTLTIGSGITFSRRLDSVINCSVTSNRRYASASNQTIGYTFPDITLSLM
                     DFESIVGLQKYITGSRLNTGFQYTVRQNGNLDWVKPKQESYTTALNPLLGFTGNLFKV
                     VSTNLSYSISQTDNITDMDTYEILKTSNSQTLNGNLSYSFRAARGFSVPFTKKKIHIK
                     NELTSSLGITYESNYDKTKGSSSSQVDRNTSRFSIIPQATYQFDANIRGGLTGRYEVN
                     SDKKTEDGTSIFGLGIWVEVNL"
     gene            complement(8620..9543)
                     /gene="era"
                     /locus_tag="PLB65_02980"
     CDS             complement(8620..9543)
                     /gene="era"
                     /locus_tag="PLB65_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOQ77520.1"
                     /translation="MGWFSTPFLFYMSISANFKSGFVAIIGKPNTGKSTLMNCILGEK
                     ISITSPKPQTTRYAIKGIWNTPEHQIVFVDTPGYLKPRYELQERMLKIWHNSLEDVDL
                     VLFLTQVDSFPTEYDKEVLHHLQYLKNPQLAVFNKLDLNPEVNREDMKQYLPDSINKA
                     FFVSAKTGENIPELMEAITYYMPYHEPYYEEDMLSDLPMRFFAKEIIREAIFHYFQEE
                     IPYSSAVLIERFQELPDKVIIHAVIWLERKSQKLILIGKNGSALQKIREYAEVELSRF
                     MQLPVYVHLFVKINPKWRKKDNALRELGFMP"
     gene            complement(9557..10810)
                     /gene="rocD"
                     /locus_tag="PLB65_02985"
     CDS             complement(9557..10810)
                     /gene="rocD"
                     /locus_tag="PLB65_02985"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HOQ77521.1"
                     /translation="MSTNLKYGNISSREAIELEEKYGAHNYHPLPVVIAKGEGVFVWD
                     PEGNRYYDFLSAYSAVNQGHCHPKIIQALIDQAKVLTLTSRAFYNNKLGEYEKYITDY
                     FGYDMVLPMNSGAEAVETAMKLARKWAYDVKGVTPDEAILIFAENNFHGRTISIITAS
                     SDPDCFEGFGPFTPGITRVPYDDADALKEYLEKNGKNVAAFIVEPIQGEAGVVVPKDG
                     YLKECYNLCKEHNVLFIADEIQSGLARTGKLLACDYDNVRPDVLILGKALSGGVLPVS
                     AVLADKDIMLLIKPGQHGSTYGGFPLACAVAVASLQVIKEEKLADRAAELGEYFRLEL
                     GKIKHPMLKLIRGKGLLNAIVIEPKNGFEAWDVCLRLKEKGLLCKPTHRHIIRLAPPL
                     IITKEQLDECVQIIKTVFDELESEI"
     gene            complement(10844..12220)
                     /locus_tag="PLB65_02990"
     CDS             complement(10844..12220)
                     /locus_tag="PLB65_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOQ77522.1"
                     /translation="MKQYSKIEILEETPVLYSILYLAIPSMLSMLVNILYNVTDTFFI
                     GKLNDPYKVAGISVALPYYNMLMAIAGIFANGGASYLSRLLGKRDIQTARETTTTAVF
                     SVGIVSIIAAALGLLFIPTYLKLTSATGLTAHYAQQYLTAIFIGSPIIMMKFTLIQFL
                     RAEGAAKDAMIGLFIGTGANIVLDPLFIFYFKMDVVGAGIATVIGQGLGMLYFCSYYL
                     KKKSLAAPSRQYLHPRWSCYKEILAIGIPSSLSQIMMSVGNAISFNLASAYTDHTIAA
                     LGVASRVFSIVIFSFIGLSIGVQPLIGFNYGAQNYGRMKKIILTANLICLAMAAFFTI
                     FFAIFPGELIAVFIKDISIIAIGKMILEAYVFAIPFAGVGMILMATLQSMGKALPAFI
                     VSLSRQGLVYIPMLFLLNHLFQFNGLIFAMPIADVITTILSFIFVYHITKQLKTSLDT
                     KPSFLIRD"
     gene            complement(12427..13677)
                     /locus_tag="PLB65_02995"
     CDS             complement(12427..13677)
                     /locus_tag="PLB65_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HOQ77523.1"
                     /translation="MNLSFKNKKLLGINGLGRIGKLLLWNQIHLRHYDGIVVNCGRVV
                     GKTIDDLIQVIEMDSTYGSIHKFLYGIAGRKAEIKVLDVEEPLLEIEGIPVKILRTAR
                     DPKDVNWLNEGVRIVVDCTGTFLDPTMHTDSGKPCLRGHLDAGALKVICSAPFKLKST
                     NSVPEDATTMIYGINHLDFDPRKHNLISAASCTTTGLAHMLKPLLENETTSRILTASM
                     STIHSATNTQSILDSVPKADATDLRKTRSVLNNIILSTTGVTKALEQVMPNIKHIGFM
                     ADSVRVPTSTVSLINLNVTFHTDLDELGEPLVNRKLINDVYLKAAEGSQKDLLLVSNR
                     QNVSADMMGTMAAVVIEAHETHTRTGFVTIPASTLATHGMVTDKILELPVTHAKIFGW
                     YDNELGSYVNCLSRLTNYVEEHTI"
     gene            complement(13710..17789)
                     /locus_tag="PLB65_03000"
     CDS             complement(13710..17789)
                     /locus_tag="PLB65_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HOQ77524.1"
                     /translation="METLKSKALAENLAVTKVSEIVLDDQAKWLLSLTEGNYGIKQRC
                     RNFLEELYHPYTNPEVVATLIRQSILGDLWFYVQLPESKQALTVIGDLYLLAAKKCSK
                     PLQEKRIIVEYLDYISALCRTEGIPKDVYSYLLDELNNWQNRSKELFIPINGTFRKCL
                     SNLVQVYECNEICFVIMKDLLLEGLEFWQRTSDIEGWGEKTSSENGFDIDYLSANLGK
                     KYYEKWLKELEDRKDFQSLQDIPSFTDIAILHRETICEFKTLTLRIKYIFYLLGLPGM
                     EELRDHLLWDLNRQLADLETELNEDEVPVMLDSVFKTLYEFTDTHLSIVLDCILTIGK
                     TVLSHKNKKHCQHLLPKIMELEFTPPGKIKIDKDWQIEVDKNHIKHLRMLLELIAIDP
                     LMCKDLLAYLIISLSQKGVFISDTDLFQKDVSAFLNSDFKPILVQTKHLLKMFPVFFN
                     EIGAEGEIRDISTSIDELGHRKDLLMHFLRKQIHTESNNTHLLLIQKILNYWTTLDYR
                     ELQDMIPEDVLQYVKNLDEMTREQSAAVNRFLKKHNLIPEELLSLSWQRVNKLFEGEP
                     EDFSLKRLKLLCYSHFLLKDKYNLDPYDIVKFLSRYSWFDAHEQNRLANSFMRKDYDS
                     SIRQLLSYIGKMNSVILDPHTTTAWENIYYKRHIAAGIPSMYGMYREPKLEAMGMIFR
                     IENVVRRLFEHNVQQLNLNYITGKTLCRIIRILELYDYAMKQIMVTNDAYSSALDMLR
                     SAQYLSNLSIDQYLDIFKLLKDSVVEIINEYYYRFYDPQLRNIGLGIAEKAKDKRKQG
                     ITETEIFAEEFYRNLLSNSFLVQGLDNFITRILESLSEMKHLFKPADIPKVMSYDPDK
                     LFFHLNKKNSRIENQVLLGSKAYFLKRMHQYEFPVPPGFVITTDLYRHRNIINTHPDI
                     SNELDSLIMHNLNILEKNTGFVLGDPEKPLLLSVRSGAPMSLPGAMNTFLNIGLNDEV
                     TYKLSRRPNYGWTAWDCYRRLIQSWGMAYGILRDEFDAIMIKYKKRYDVELKTQFSSA
                     QMQEMVQDYKNVLFEHNLELEQNPFMQLYKAISNVLDSWNADRARLFRQKLHIADEWG
                     TAVIIQQMILGNISLESGTGVLFTHYDFSKEGGICLNGDFTLCSQGEDVVAGLVHTLP
                     ISETQRLHSQTPVADSLENTFPAIYNELLRYAKQLIEDKNYPHQEMEFTFEGPSPDQL
                     YILQTRNQVIQKAPDYKVFGSTKGDMKKLAYGIGIGKGTINGIVVFNQEDIKKYADKG
                     EALILVRPDTVPDDMELLFECQGLLTSRGGVTSHAAVTATRLGLIGVVNCRDLVVSDL
                     DFNCRIGDTYLKAGDKIALDAISGAIYLGHYPLVSVHCL"
     gene            17939..19144
                     /gene="dnaN"
                     /locus_tag="PLB65_03005"
     CDS             17939..19144
                     /gene="dnaN"
                     /locus_tag="PLB65_03005"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOQ77525.1"
                     /translation="MEKIIINQSEVMMRLAIEKKDLLAHIQHLVSIVPSKNVTPILNN
                     YLIEVSEETNMMRISTTDLTLTVVVEFPAAVSEGGTIAVSAHHFNEIINYMPDAVINL
                     WQHDDLLMIQSGKVDFNLLIADHTLFPVIPEPKINYPITVDAALFNRMISKTYFAVST
                     DVNRAILTGVCWKIYPTYHMMVATDGRKVAEIKVLNSSLLPSLENNDNQGLSIFTEQN
                     EVYVEKVIPLKTLLFLQKIFSEDVKELKVSMEHNRIMFLYGEYFVSSQVFEHKYPEYQ
                     KAFPTELPNKFVIDKESLITAIKRVALIAPDENFRIHFDIDNDRFEVNTTNRDTGDAK
                     QNMDDYVYAGTSTGISFNYKYMLSILEAIDTEKVVIKLGSSKDPMMIYNENPIPDQEI
                     TFLLMPLRS"
     gene            19150..20223
                     /gene="recF"
                     /locus_tag="PLB65_03010"
     CDS             19150..20223
                     /gene="recF"
                     /locus_tag="PLB65_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198307.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HOQ77526.1"
                     /translation="MNLVNIELENFRNYRNSTYSVGPLGSLIIGPNGCGKTNLLEAIA
                     YCSIGKSIHFHHDEELLHFGERFFRVQGSFISDVGTPIKVALGYAEQRKLLKIDDLPI
                     RQLSTLFEIVKIIYCAPEDHLLITGSPRFRRQYFDLAIAQLYPPYITALRHFLHMVQQ
                     RNAMLKKNYTKEEQISWNTSFATSLAEVWNYRNRYLTHINTAFKEIFKDVFPTSASIT
                     ISYLPSLKMPLDSSIDDIVQYLSHLAEREKLLQRSLVGAHLDDYEFRLEGKKMRSYAS
                     QGQKRIAVIILKLIQAHLIEKVTGIKPILLFDDIFAELDAFHSEAIRNCINNHYQVFI
                     ASPKEDICLIWQGYPILRLGEEH"
     gene            20224..21450
                     /locus_tag="PLB65_03015"
     CDS             20224..21450
                     /locus_tag="PLB65_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ77527.1"
                     /translation="MKLNRNILGWMFYDFANSAFTTIIVSVVYSVYFIKSVVGGPSGY
                     GEMLWGRAIGLSMTLVAISAPILGAVADYSRAKKRMLFINCYLTVIFTALLYFVKPGD
                     VFLGMLFFIIANFGFNSANVFYDAFLPEICQPEDIGKVSGFGWSLGYVGGLVSLLVAL
                     VLVEINIRLVFPAVALHFFLFSLFTFFWLKEFKRPSKPSNYYRIAWQRVSFSLKNIAK
                     LPQLLKYIISYFIYNDGITTVIVFASIYGAERFSMTTQQMITYFILAQFTSIVGAAFF
                     GWLTDKLNVKISLSIALMIWIVVVIWAFFCGSATEYYCVGLAAGLAIGSSQANSRTML
                     SLLTPRERQAEFFGFYTLTGRLSSIIGPILYGWIAHKTGDIRYAILSLIFFFVTGWIL
                     LQSVNLQEGIKQAEMQ"
     gene            21505..22563
                     /locus_tag="PLB65_03020"
     CDS             21505..22563
                     /locus_tag="PLB65_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HOQ77528.1"
                     /translation="MKKKQAITLMILMLLLFFIGSCSKNKPVLYIFNWSDYIDPELIE
                     EFEQQNKCTIKYSTYDSNENMLTKIMSSRESFDLVFPSGDHITIMRDSNLLEPLDISK
                     IPNYQNLDSTLLEKAASFDEGNKYAIPYFWGLTGLIYNKRYVPDEVINSRSWNILGNA
                     FFTGKNKVTMLDDAREVVGAALIYNGYDLNDSSVMALAAAEKTLAQWDKNITQFDSDS
                     YKNEVPDGTTWLAQAYNGDALQVIDNSPDLGFILPVEGASLWMDNIAMLKSSNNKELA
                     YKFINFLLDADIGKRNTEYCLYATPNKAAGNLLPETLKNNTLIYPGDEYLNKCYMINA
                     LGEDVKKIDKLFEAIKLN"
     gene            22588..24369
                     /gene="aspS"
                     /locus_tag="PLB65_03025"
     CDS             22588..24369
                     /gene="aspS"
                     /locus_tag="PLB65_03025"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOQ77529.1"
                     /translation="MLDNLGNLTRSHYCGDLNTDHIGQNVTVMGWVNKRRDLGGLIFI
                     DLRDVKGILQIVIRPEQEEIFAKAEKVRNEYVIAVCGIINARSEQNINPSIPTGKIEL
                     VAEDFYILNDAQPLPVQFSEAAMAEEDLRLTYRYLDLRRPKLQNIIITRHRIMQIIRD
                     FLNNEGFYEIETPILMKSTPEGARDYLVPSRVQPGKFYALPQSPQMFKQLLMIAGFDK
                     YYQIARCFRDEDLRADRQPEFTQLDIELSFVTQAQIFDLLERLMYKLFKGILDVSLSI
                     PFPIIPYAEAMENYGCDKPDIRFGMKLNDISTIVANSSFNVFSSALQNGGVVKAIAIP
                     GAANFSRKQQDELIELAKHLGGKGIAFAKVTENGLEAGISKFLSLEETEAIIDVTGAV
                     TNDLIAIAADNYEMVCKVLSGIRYYLAEKLGLIPEDNYAFCWITDFPLFAKNAETGAW
                     EPAHHMFTLPKEEHIPYLDLPEKIGEITGQLYDLVCNGVELSSGSIRCHRYDIQKKIF
                     DILGFSNKELQDRFGFFLEALKYGTPPHGGIAPGLDRLVMIMTKAESIRDVIAFPKSL
                     KAADLMTQAPSEVEPQQWKELHLAPTK"
     gene            24336..24938
                     /locus_tag="PLB65_03030"
     CDS             24336..24938
                     /locus_tag="PLB65_03030"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HOQ77530.1"
                     /translation="MERTTSGTHKVAVFCSGLSRGSNLCAIYKYFADNKLPIEIALVI
                     FTCKDAPAIQLAEERNLNYQIIQARDMQSFEKRAMELCRQHSIELIALSGFLKQLSPN
                     FIESMQIPILNIHPALLPKYGGKGMYGMAVHNSVFAAGEKESGVTIHLVNSQYDNGKI
                     VAQEKVDISACKSPEEIAQKVLDIEHKLYARTIKEYLLNH"
     gene            24935..26203
                     /gene="mtaB"
                     /locus_tag="PLB65_03035"
     CDS             24935..26203
                     /gene="mtaB"
                     /locus_tag="PLB65_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HOQ77531.1"
                     /translation="MKRIAIITLGCKANQAESSIILNQFEPFKLVSWQEEADIYIINT
                     CTVTNRTDYKSRYLIRQALTKKAKNPSIQIIVTGCYAQRYPEEITKLGNIDFVVDNQQ
                     KLNIADILAGNSYEFMDIMQAKDFACKLTNKMYDRTRAFQIIQDGCNFNCAYCAVPYG
                     RGRSRSATLEQILQQARLFVKNGYKEIVLSGINLGLYKDGDNDLTDVLRCLNDLEGLE
                     LIRISSLEPQLFNYKLINLLPSLPKLCPHFHIPLQSGTDSGLKRMKRHYTTTTIKKLV
                     FDILDKIPSAAIGMDVITGFPGETEEEHKFTCDFICSLPLAYLHTFSYSKRKGTPAAE
                     MTNQIPKKIKNQHSNELIHISQELTANYTNLLYNNNIPLRGIVENSENGCSDFLSDHY
                     VRVYLSKEYPAGELVKTSAKEVKIAGESDV"
     gene            complement(26372..27148)
                     /locus_tag="PLB65_03040"
     CDS             complement(26372..27148)
                     /locus_tag="PLB65_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ77532.1"
                     /translation="MKSQKVILPLLFFIFLIILWQFISAKGVIAFWVVPSPLQVINVF
                     MKNPGLIWHHLKPTLLEAITGLIISIILGSITAMAMNLSSWIKQILYPYLIISQTVPI
                     IAVAPLLIIWFGYGISSKIFAVVLMCFFPIALGLYDGFRSVSVDQIRLLKSMGATPYK
                     IFKLLKIPAALPNFFTGLKLAATYSVMGAVIGEWLGGTSGLGIYMTRATKSYQTAHVF
                     AVIIVIVAVSIALFGMVALLDRILLKWKYQTIDEYLEPIK"
     gene            complement(27145..28086)
                     /gene="murB"
                     /locus_tag="PLB65_03045"
     CDS             complement(27145..28086)
                     /gene="murB"
                     /locus_tag="PLB65_03045"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOQ77533.1"
                     /translation="MNITTLSILQQEYPKLMEDGIIQSNVPLKEHCSFQIGGPAEVFC
                     TPYTQNQLLDLLRFCLNHKTPYFILGKGSNLLISDKGLSGIVISTERFTELTVEQEYI
                     TASCGVTLKDLCNYACENGLSGLEFASGIPGSVGGAVFMNAGAYNYEIKDVLHSSVCI
                     LPTWESINSLSPILYLKVEEHNFSYRHSVLQDKELIHLSSVFHLKRDNSETIKRRMDE
                     YQQKRQQKQPLELPSAGSVFRRPEGFYTGRLIEDCGLKGFRIGDAAISEKHCGFIVNL
                     GSATAKDVLQLIQHIQEVVYEHYNVKLQTEIRFLGEM"
     gene            complement(28083..30968)
                     /locus_tag="PLB65_03050"
     CDS             complement(28083..30968)
                     /locus_tag="PLB65_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOQ77534.1"
                     /translation="MESENGKDLNSENVSEELLLSQLEFVAIDIETTGLMPARDEIIQ
                     IAAFRFKGNEVVDSYVSFVKPKGKVPKFIEQLTHISPEELKLSPPLKEVLKEFCDFIG
                     NTPLVGHNINFDLSFINQSLVENGSFPLINVFWDTMEIARMYLPFTTDHKLSTMVSFF
                     GIKLDNAHRADSDALATGYLFNALTNYALAHFNYMLNARLQDLSLQANVESGLTEYLR
                     WLVKQQRATVLSGKKPIPPKNELNNVIEHNVSGSIDSIPEIFAEHGLFANKFPNFEFR
                     SGQMIMAEQVANAFAKQDFLVVEAGTGVGKSFAYLVPAIDFSNRNKTKVIVSTNTKNL
                     QEQLFFKDLPQLRKIIPLPFKAVLVKGRENYICERRWQDLLQEQTRGISAYDARGLLN
                     LLVWKELTHTGDISENSSFDKNYYSVLWHKICSDRYLCGGRKCLNYHNCYVMKLRKHI
                     ESASIVVANHSLLLADAQMDNSTLGEYQYLIIDEAHNLMSAASKNLGFTFSYAELNSL
                     FNQLAHSGKKYTGGFLPQLIQTVHKSILTTDQKTHIESLCTKLMLETDEQRKVITSLF
                     NLAANRCFEAKSYNKLRIKDIADFQDIYFFLKELSIGFKNILKDLVALDNVLRTVKSN
                     QIANLEIIKDNLNGYLQRYRETETLLLELLNPDLDNYALWIENIPRPERNIPSSTFCY
                     APVEVSEPLNRLLYKNIPCIVFTSATLSIRGSFKFFLNQSGLSFATDKKVIESIVESP
                     FDYDKQSLLLIGSFLPEHKDRFFQSQALGCLEQILTTTNVGTMALFTSYKDLDAVYEH
                     LSDTLYHSKRPFFAQGKGGSRNSILDDFKKSKNAVLLGTSSFWEGVDIQGDSLSLLIL
                     YKIPFQVPSEPLVEALIDKLERENKDSFMHLMLPNALLRLRQGFGRLIRSKTDRGIVL
                     IMDSRVSKKKYGQYFKQILPGRCIELKNEQQLISEIGKFFSSLPSNL"
     gene            complement(30986..35269)
                     /locus_tag="PLB65_03055"
     CDS             complement(30986..35269)
                     /locus_tag="PLB65_03055"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOQ77535.1"
                     /translation="MAENITQQLDAYIINKIALSRAMGYLRESSKSHLIELTGKSGSG
                     KSHYVQPLMDSVSDVYEKRMCFSPHPLALNHFTELIQVITEVSEADLYGYYNEFRDNI
                     GSDSKYDFFYYITERLNGMELFHSLLLVIDDCDVLDAYTRDYLQYLVQYLPETGIQIV
                     AFTQTRLFPFSEMENIPALEIEDCQRLLQLTFPGTDFSNSSDSEIFQKITSGNLIIIE
                     KVLREMQAQKTRNKFNLGPYLEKTFDAQQIYHEMLESLSPSQKEILTVMYILDGLDTD
                     ELYQTLSKPDDLKADLTLLYDLRLISNPGTVCSIQKKKAFSQWLNDNKDQIGSTIFNQ
                     LIEYLQKDKRYPQILASLQIMNNKYNAALFAKIVSELTLINDFESVLTLEEYILAHTK
                     NPTGKLTSTQKIAEAFSAINQKDKAVDYFRQALHICTENNFPAEQIVYKLANDLYAIN
                     SSAFALEIIKQYSPATIDPYWKVKILLLKSDIQAESEAFNDAFNTLDNASHSFSNITN
                     QQQRHSVQAEAKKIRGKIHYYINEWEQAEEVFNEAETLYSLAKDHSGLAAIYNNLGVL
                     YMFQGFWEKSEAYFLKSLVLEKQYYNLNGISVCYNNLGGLMDDKGDPEKSLDYLEEAL
                     KIQRLLAEPYNITNIYNNIGVTLMDHNEYDRAEDALKKSLEIANEFGFQRNTIASLNN
                     LGGLYFKKGDWAESISYYEKAIKLSQESGFNEGLLRSFNNLGEVYEKQDELNLAYDLY
                     IKGLELLPAVSDDYIKAELYGNLGSVLTKLHKFKEAYRYLVESLDFFKNIAARDKIIE
                     GYLKQAYYFILTHNVESADYYLSEAQKLAAEQNLPFEIGRAYYLRAVLERKNLEAAKN
                     YLNEAIKLFVEVGSNYELSLVNYELAGVLLDMEEWEQALQILKNNRKIIEKYGSIKLL
                     EQNDILMQKISREYASQMEEAKFEENLLNQFYEITQKLNTITDLDVLIEQSLTSLIEI
                     ANADGGVLLLHANPNLPDAWEYKIYNNFSPDDKYHDHFLNICADVYQNNKLENIKQPH
                     FAPSYNNILVLPLSIRKNTMGVILIFCKSGSKYFSERIINLLSALANQIIVIIDNIRS
                     ANLEKSHAIIREQLTSNNIYADIIGKSPEMMKIFEIIEKVKDTPTTVLLEGPSGTGKE
                     LIARALHYSSNRRNKAFVAQYCGALPETLLESELFGHVKGSFTGAAYDKKGLFEIADG
                     GTFFLDEIADISQSTQAKLLRFLQEGEVKRVGATKTEKVNVRVVCATNVPLMERVKRG
                     DFRLDLYYRLNVIRIDVPQLKNRTGDIPLLAIHFLDKYTKRMDKNVLGFTKEAMDILE
                     QFDWPGNVRQLENEIERAVTLVEPNTFIKPSDFSEEVFHHIEYSKTIAMLSGKKSLKE
                     AIEELERKIITQSLEKFQWNQTQAAKELGLSRQGLIKKMQRYNLYKEED"
     gene            complement(35434..35883)
                     /gene="tnpA"
                     /locus_tag="PLB65_03060"
     CDS             complement(35434..35883)
                     /gene="tnpA"
                     /locus_tag="PLB65_03060"
                     /inference="COORDINATES: protein motif:HMM:NF033573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HOQ77536.1"
                     /translation="MPASFCHLAVHIVFSTKNRKTWLKKPLADEMYGYLNGIIKNLNG
                     IPLAIGGIEDHIHILCLAPKEMSIVEFMAKLKANSSKWFREQTRQDFHWQDGYAAYSV
                     SKSNIESVKNYIEHQEDHHHNITTEEEFDALLKKHWDPDKLKEKQMI"
     assembly_gap    36282..36381
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(36483..36926)
                     /locus_tag="PLB65_03065"
     CDS             complement(36483..36926)
                     /locus_tag="PLB65_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77537.1"
                     /translation="MKVTVKDGIKGFSGKLDGAIYYYHPRLKRTLMRRAPQMPIQEKN
                     LEYAAIAKQIKAINPSEAYRSDFKNYLNLLRDKDDSVRVPSWYSLFVKMLWAMQAKYP
                     EQVNLKTITKEQILEQQLPCLSVKTAVEDGLLTKVPGYQYLDKEI"
     gene            complement(37268..39394)
                     /locus_tag="PLB65_03070"
     CDS             complement(37268..39394)
                     /locus_tag="PLB65_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77538.1"
                     /translation="MSVVKINIILGFIAFTMLLVPKAYSTQIGDYPQQIETQELLRNI
                     QEKYQDLTPISSDIGVLGDKDNQFSPSPFADSVMFYADEEYLYCIYIAQVDSSLERGR
                     IVKKDGDTQSDAIAFTLITQPNQFFAYGFEASPLGVLSDYTLDTSFNADWSWNSDSRL
                     TSTLSDNNWLVELRIPWCDLRMGSKAPYTLGVYVSRFCYQGKKSYRYPYVQYTSGPFF
                     FQNGAQIIINNPIGKQGNFKADAYYVAIYDSKENRGNIWYKNIGGNLNIKPYSNASFK
                     ISIKPDFSDTPLDSAVDNYNSKNPPQIAENRSFFLEDYDLLAVSSDLWYTRNIISPIF
                     AGKYTRVSQDRAVALVFLRDDPSNNLSNGDFFNALGYSRTFATSKVNINLYGRQNDDF
                     HNELAHASFSYRPLEPLEILPIVNFSYMKQDSLSSMGYDAGITLTNTHSDYTFWVASE
                     LTDTNFRADMGSFYNQDRFQNTVGFFYDKPRNGFLSRLQSNVQWQLDNTLSSGELMSS
                     SGNYSQLVYLDNNNYAIAMETYYRAEKFQSQLYHPYKATLGLNWLKNSRLTAFASVSG
                     GKEIVYSLSEILPVINYYGVIQINLLEKLWIYYNLTYVRYSHSKLDYWDPEYNLMNLI
                     NNIVLQKNIILSQGLSLTNYNITMKPDMEYLGNLGYYLNLDWNVGSNSHIYAGYKSQY
                     SHYSIQSDNDLITDASNFYLKIQLGF"
BASE COUNT        12818 a         7159 c         6818 g        12605 t
ORIGIN      
        1 atctattaag aatattgatg atgaaacttt aagctgatac ttgatatcat acccttcttt
       61 attgtcttct ctgaacaaac cagaattcat accattgtta ttactaaaat aaactccgcc
      121 taaattttat gttcctttta cagataatta tctgcgtttc aatgaagtat aatttaggta
      181 attgggatag atgagcaaaa cgcaaaagca aaattacctt ccaagatacc attagcaatt
      241 ttcagttatc cgatttacgg tctaaagtga acgaaaataa ctccctgcaa gaaagcatct
      301 atttagagtt tatctaccct taatcaagcg ttaattgtta acgcttgatt aagggtagat
      361 aaaggcttaa ttaacgcctc tacctaggtt ggttaaaatc attatatctc aatatagtaa
      421 aattatattc atattatgaa gcgtactatt tggaggtaat gtcagttatg taataacact
      481 tttacgaata caaaaattta ggaacgacaa tccttcacaa aacataaaat agtatgattt
      541 gctttatttt taaactaaac tataggcttg aaccaagcta aattctaagg gataattgga
      601 gacaaattaa gctacccaaa tagaattagc tgaaaaaaat acgaacttta tctaatcctt
      661 tccaaattct ctttagcaga tggatctact tggtatacat attcattttt ttcacagggg
      721 aaatccgagc attcagcaca gcttatgtat cctttttcta ttacacataa acgaatggga
      781 cacaatccac accaggaaaa ttttgctcct tcgctcatac aaccatcaca gttaatatct
      841 tcaggatgaa tttcaccttg ataatgttcg ctccaacgtt ttgcgatatc ttcttttctt
      901 gcttcatcat cattctgagt ggcaatatat gtttcacatt tgaaacaatc aactccgcag
      961 gcagagataa cttttttcat tttttctcct cttccggtga taaaaaaccg gtactttttt
     1021 agatagatat tctgttttaa tcgctacaaa agaattaagg cctaatggct ttttcatttt
     1081 tttactgcat tcaaaataga attgaagttt tcttattttg gaaagccaaa tcttgtcaat
     1141 aatttattct acactccttt acactccaag atataaagca gttgagaaat ctattgacag
     1201 aaatgttata tgggaaataa taacttattt tagaagcttg cgcttgtttt gttatcttta
     1261 ccagaaatga atttgagcag ggattatgtg taatgtaatt atacgagaac cagttatggt
     1321 agtggacata ggaaacacca atatcttatg tgccatctac cttgatggag aacctatttg
     1381 gacggcgcgt ttcacctcga gtcgggaaaa aacttctgat gaatattata ttcttctcag
     1441 tggtttaata gaatcttcag ttttatctga agaggaaaaa atcgctatag aagatattaa
     1501 ttatgttgct ttgggaagcg tagtaccaga acttacacgc gtttggagac atttatttag
     1561 caagtatttc aaggttgaaa taactgagat taatgcgctc agtcctttag gtattagttt
     1621 cagagtagaa aatccgtctt ttattggagc agatttaata gctaatgcct atgctgcctt
     1681 gcacaaatat aaaagttcct caataataat agatttagga actgctacta cagttgaact
     1741 gatcagcaac gaaagaagat ttgaaggagc cataatagct cctgggatga aacttggtgc
     1801 tgaaatatta tttcaaagag ctgcccaatt gtatgaaata gaacttgttc aaccgcctgt
     1861 tcttttaggt ttaaacacta cagatgcaat gctttctgga gtagtttatg gtcattctat
     1921 aatgttagat gcccttgttg ttaaaatcaa agaacagtat tcacagctag caccaattcg
     1981 aacaatcttg actggtggta tggcttctct ggtgaaaccc tctatgaagg aaattgatgt
     2041 tgtagataga cacctcactt tggatgggtt ttatttggct ttttgcagga ttttacagca
     2101 aaaatagtac tttatgctga aaaagctatt cctttgtctc ttacttatag cgttcacctt
     2161 actgtgggct aatgaaccta ctggagaagt taatacaaac cctgagcaag aagaaacagg
     2221 acagaaggga cttttcccgg tgaaaccttt cagacaaaaa agggaaggac aacatcaact
     2281 tgatccacta acagaaaaag caatgtatcc tcgtttgtat attcccgatt ataatcctgt
     2341 aataaataat cgtattgaag tttataaaat caataaagca atgaattact ataaagcaag
     2401 accactggaa ataccagctc ttgcaccaga atataaagaa aagatagatt ttactaataa
     2461 taaagtaatc ctcagtgtgc aggttggcga ctataaaatt gctccagatg ttattattag
     2521 ctttgatcgc tattttagta atatgcaaat gaaagccttt cacaaatcta ttattgccaa
     2581 tataaaaaca caagctcaag caacccaaac agttagcagt ggtctattta aagatcttac
     2641 cttgcttcca gaaatagcta tgcccaaagc tatgcaaaaa gttttaggtt cttctgctgg
     2701 acgattaaat ttagatggta cccagaaact tactctacag gcaagtaaca cacgcagaaa
     2761 acaggttcct atttatgatg aatcaggaaa aaatgttttc gacctaaaaa tggagatgga
     2821 aactaaccta aggctgtccg gaacaatcgg tgacaaaata gcagttaatt tcaaatacaa
     2881 ttccaaacag gatgaacagc tcttcgatat gaataatgta aatgtaaaat ataccggtta
     2941 tgatgatgaa tttgttcaaa gtatcgaagg cggaaacata gctctttctt taggtggatc
     3001 gcgctatgtt agttattctg caagttcaca gggacttttt ggtatcacca gtaaattcaa
     3061 atatggcaat ttggatttaa atcttattgc cagtaaagaa gagagccaaa aaagcactca
     3121 aacctatgta ggtaaaagtc aagcagattc ttctacagta agcagctgga aatatgctaa
     3181 acgaacaatg tactatctgc atgatcccta tcagttatat caattaaaaa caggttctaa
     3241 tatacctcca ggctgggtta ataatgcaat tgatactgct cctgatggct catggcttgt
     3301 gaatgaaaac tatttacccg taagtggaac cgtgcgtttg ttttatgacg atgctaatta
     3361 tacaaataac actgcagcag caaaaggaga taccatctat tattctaatg atgttagacc
     3421 tccttatgaa ccctggtatg atgaattaat tgaaggaaca gattttgtta cggattacga
     3481 tagcggaatt attactgttc taaaaaatat tgatagaacc gctactttag gagtgcaata
     3541 cactcgtcga gatggagttc aagttcctcc tccaaatcct gatacaagta ttttacatgt
     3601 aaaagtatta aggaaaaaat accaagagta tattccttat aactccaatg atgaattaaa
     3661 tacctggcat ttacaaatgc gcaatatcta tgatatgggc agaacaaata taaagaatga
     3721 tggatttgaa ctgcaagtat atactgttaa tgtagattta actcgcaact acaatctacc
     3781 cgataatctt gtttttggag aaatatcaac atataatgac tatctgaggt taaatgctaa
     3841 taatagtccc gatggcatta tcaatggtga tgatgctact gtcaatctta ccaatggttg
     3901 gattattatg ccttttatcg aaccgatgca aggtttggga gatggtataa tctaccgtaa
     3961 agaaaatgaa gatgaatatt cctatcagga ttcaaccagt atttttatta atattaaagg
     4021 taaaattgga cgagaagcta tagaactttc ttctatggga gtgttaaagg gaagcgttag
     4081 agttagagta aacggtgtag aacaacgcga aaacactgat tatattgttg attacgattt
     4141 tggcagaata accttcttaa ctgctgcggg taaagatcct gatgccaaaa tagaaataga
     4201 ttttgaatct cgtactcttt ttagtgtagc tcaaaaaaac ttggcaggaa ttagagcaaa
     4261 ctggcaaatg aatgattata ccaaactggg gggaacttta atttaccgaa gtgagagtgt
     4321 agcagataaa cgacctcgaa ttggtaatga gaacattgaa atgataatgg gcgatataga
     4381 tggcgaagtt actatcaaac cagaatttat tactaggggt ttagatgccc ttcctttaat
     4441 taatactact gctccttccc aaatatcgat gtctggggaa attgctttta cgctgcctaa
     4501 tatatacgga gatcctgata gtaagaaaaa agaagcatat ctggatgata tggaatcaat
     4561 tatggattct tatcctttag gagttactta taataattgg atactgggta gtaagccttt
     4621 tggaacttct tatgccaaag gaagaataaa ttggttcaat cccaaagatg taaaaagacg
     4681 cgatattgag gctgaagcta ctttaacaga tcaagaaaaa gatgaatatg taactgtgct
     4741 tactatgatt gtaaaaccca atcctgtgca aatcccaaat tctacaacac attcctgggc
     4801 tggaattatg aaatatcttg gtaaccagct cgatttctct cagaaaaaat atctggaagt
     4861 gtatgccaaa ctggagccta taaatcctaa cacaaatatt tacccaaatg taacaatgca
     4921 tatagacttg ggagatatta atgaggattt ttacactgaa tttggtggtt atggagtttt
     4981 aaacagcgaa gatgtaaacc gtgatggagt tttaactatc agcgaagata ttggtttaga
     5041 cggtatttcg aaaaaccaac ctggtcacga tcccaatgat attgcctatc ctgccagtgg
     5101 gaatgattat tctggagtga atggaacaga agataaccgc atattagata ctgaagacct
     5161 tgatggtaac ggagttttaa ataccttaga tcgttacata agttattctt tttctctctc
     5221 taatcccgat acttctatag taatagattc ctataatcag tggcgaatgt atcgtatacc
     5281 attaagtgat gaacaatact ttcaacttgt aaatagctca ggaacaaata ttcaaccaag
     5341 tttacaaaaa gtttcgtatg cacgtatctg gttggataca gattgtacta tggatataaa
     5401 agttagaatt gcagatattt ctgtagttgg aaataaatgg caagacaatt atattcgtca
     5461 attcaaatat agtagtgcac ctccaccaaa cagttatgtt caatattata atcttatgat
     5521 agatcctact gttttggcag ctaaaaatac tagttttatc tctggaattg taagtaatca
     5581 aaagaacttc tctcactata cttctcctcc gggaacttgg tatacagaag aaaatcgaga
     5641 atcggcagaa tcagctctaa ccttagaagt taaacatctt caacgcgacc aaatgtgttt
     5701 actcagacaa aaaatgctgg atacccaaaa tttgctctct tacgataaaa tccgtttctg
     5761 ggtatatcct gaagctgctc aaggttcata ttcacatcta gattctttat atgtcatttt
     5821 ccgaataggt gctgattctt taaactatta tcaagtgcgt caacgagtgc cagttcatcc
     5881 ctataataat aaaataagtg ccaaagattg gattcagctt gaatataatt tgcaggacct
     5941 tatatcctta aaagaaatta acccaaatgc agatgcggat tccctggtta atattgttcc
     6001 tggtgatgaa ggaaaaatag gaaaatacta tagaggtaaa cccagcttaa taaatatcag
     6061 agaaatttat ctgggtgttt atgttcccgg aactcatttt tatgctgccg aagaggaaat
     6121 aaaagagttc agcgggacta tttattttga tgatataaga gttgctgatc cgtacgaaga
     6181 tataggaatt gcaaaacgtc ttagtcttag cgggactttt gctgatgtag ccacattaaa
     6241 tatagattat gaagaaaaga gcgaaaactt taatactacg atacaacgcg gacgcagcaa
     6301 tacttttacc agtgtccgat ctctcaatgt aacaaataaa atatttgtta ataagctgct
     6361 ccccaattct tggaatatgg atattcctat ttccttaaat cgtaattatt cgctaggaat
     6421 tcctcgtttt cgtgcaaatt cagacctttt aagagctaat atggctgatc ctgagctaaa
     6481 agaaattgaa aagacggaac atttagctta cagtgcagat ttcgggctca gccaaaaaca
     6541 accaccaaaa agcaaaattc tgcaatatac tatataccga acttctatta gcggtagaat
     6601 tgaaaactcc tataataata cttcaactgc aatagatact cttttggcat atcgcggaac
     6661 tttgaactat aatttgaatc ttcctgctga taaaactagc ttgaaattgt tcaatgatta
     6721 ccgattaggt tatttcccaa acaccttcag caatagtttt acctttagta gtaacgaacc
     6781 taaaagctgg gatagggtta ctactgtaga tagtttggct tggaataaac gtagccagac
     6841 aactgatacc agaactataa caaccgataa taatatttcc tggaacatat taagcgatgt
     6901 aactgctaca gctcgtctaa acacaaaaag agacctgaaa caaaaagact atatttacga
     6961 tataaatgta ggtaaacaaa cagaatatgt gcaagatcta ggattgaact acagtccaaa
     7021 ttacctgcca cgcattttta actttacttc ttctgcttca gctagatata cagatacaca
     7081 gcgaaaatat actcaatata ttggtggttc agcagtagac acatatcaaa gagatggtaa
     7141 tacaaaccgc tccattcgta tgaatctaac tttgatgaat tcctctctgt ttacagaatg
     7201 gactacaaaa atgatgagta aaatgcctga aggaaccatc tcccccaaag tcaaagacaa
     7261 agataagtac cctaaatccg aaatgtctga agaagaaata aaaaaacaag aagaacatca
     7321 gaaagaagaa gaaataaaaa aacaagaaga acatcagaaa gaagaggaga ttaaaaaaca
     7381 ggaagaacat caaaaagaag aagagattaa aaaacaggaa gaacatcaga aagaagaaga
     7441 gattaaaaaa gaagaactga agccagaaga ggaacaaaaa cttagcgaag aagaaataaa
     7501 taaacgccaa gatgaaattg cccgtttgaa agctgaaggc aaatttgatc agctatctga
     7561 agaagaaatt aataaactgc ttgcagacat atctgctgga aaaggtaaaa aagaaaccca
     7621 agaaaatgat ggagagaaag aacctgaagc taacggaccg ggatttaatc ctctacttac
     7681 ttttgttagc gctctttcgc atattaagaa cattaatgct tcctatcaaa acacatatgt
     7741 tatgaattat gcgcgtaaaa caaatccctt cccttttgct ttccaattag gaattccaca
     7801 ttgtgttccg tatgattctc tggaagcaat ttccaatgat aatactctta ctataggtag
     7861 tgggataact ttctctcgca gattagatag tgttataaac tgctctgtta cttccaatag
     7921 acgttatgcg agtgcaagta atcaaaccat tggttatact tttcctgata ttactttatc
     7981 tttaatggat tttgaatcaa ttgttggttt acaaaaatac atcactggtt ccagattgaa
     8041 tacaggtttc caatacacag ttcgccaaaa tggaaatttg gattgggtta agcccaaaca
     8101 ggaatcctat acaacagctt tgaatccttt acttggtttt actggaaatc tctttaaagt
     8161 agtatctact aaccttagct actctattag ccaaactgat aatataacag atatggatac
     8221 ttatgaaatt ttgaaaacca gtaacagcca aaccttaaat ggtaatcttt cttatagttt
     8281 tagagctgca cgcggctttt ctgtcccctt tacaaaaaag aaaatccata taaaaaatga
     8341 actaacctcc tctttgggga taacctatga aagtaactat gataaaacca aaggtagttc
     8401 gtcatcacaa gttgatcgta acacaagccg cttttcaatt ataccacaag cgacttatca
     8461 atttgatgct aatatcagag gtggtttaac aggtaggtat gaagttaatt ccgataaaaa
     8521 aaccgaagac ggtacttcca ttttcggatt aggaatatgg gttgaagtaa atctatagaa
     8581 tagaaaaagc caagatggaa ataaacgaaa tataattatt tatggcataa agccaagttc
     8641 tcttaaggca ttatccttct ttcgccattt tggattgatt tttacaaata gatgtacata
     8701 tacaggtaat tgcataaagc gagataattc aacttcagca tactctcgaa ttttttgtag
     8761 agcagatcca tttttaccta tcagaattag tttctgactt ttccgttcca accagataac
     8821 tgcatgaatg ataactttat ccggtaattc ctgaaagcgt tcaattagta ccgctgaaga
     8881 ataaggtatt tcttcttgaa aataatggaa gattgcctca cggataattt ccttggcaaa
     8941 aaaacgcata ggcaaatcag agagcatatc ctcctcataa tagggttcat ggtaaggcat
     9001 ataatatgta attgcttcca ttaattcagg aatattttct cctgttttgg ccgacacgaa
     9061 gaatgcttta tttattgaat ccggaagata ttgtttcata tcttctctat ttacctctgg
     9121 attgagatca agtttgttaa atacagctag ttgtggattt tttaaatatt gaagatgatg
     9181 aagtacttct ttgtcatatt ctgtagggaa gctatcaact tgagtgagga aaagaactaa
     9241 atctacatct tctaaagagt tatgccaaat tttcaacatc ctttcttgaa gctcataacg
     9301 aggttttaag taaccaggtg tatccacaaa aacaatctga tgctcaggag tattccatat
     9361 ccctttaata gcataacgcg tagtttgagg tttgggtgat gttatagata ttttttcccc
     9421 taagatacag ttcatcaggg tagatttacc tgtattaggt ttccctataa tagctacaaa
     9481 gccactttta aaatttgccg aaatactcat atagaataaa aaaggggtgg agaaccaccc
     9541 cataagctat ttattgttat atttcacttt caagttcatc aaagactgtt ttgatgatct
     9601 gaacacattc atccaattgt tctttagtaa taattaaagg tggagcaaga cgaattatat
     9661 gacgatgagt tggtttgcat aataaaccct tctctttcaa tctcaaacaa acatcccagg
     9721 cttcaaaacc attcttaggc tcaataacaa ttgcatttaa taagccctta cctctaataa
     9781 gttttagcat aggatgcttt attttaccta gctctagacg gaaatattct cctaattcag
     9841 ctgctcggtc tgctaatttt tcctctttaa taacttgtaa agaagcgact gctactgcgc
     9901 aggcaagtgg aaaaccacca taagttgaac catgttgacc tggtttaatt agcagcataa
     9961 tatctttatc ggctaaaaca gcagaaacag gtaatactcc accggaaagt gctttaccta
    10021 aaattaaaac atcaggacgc acattatcat aatcgcaagc taaaagttta ccagttctgg
    10081 ctagaccact ttgaatttcg tctgctataa acagcacatt atgttcttta caaaggttat
    10141 aacattcttt tagataacca tctttaggaa caactacccc tgcttcacct tgaatgggtt
    10201 ctacaataaa agcagctaca ttcttaccat ttttttccaa atattctttt aacgcatcag
    10261 catcatcata aggaacccga gtaataccag gagtaaaagg accgaaacct tcgaagcaat
    10321 caggatctga agatgcagta attattgata ttgttctgcc atgaaagttg ttttcagcaa
    10381 aaataagaat agcttcatca ggagtaacac ccttaacatc ataggcccat ttacgagcaa
    10441 gtttcattgc tgtttcaact gcctcagcgc cagaattcat aggtaaaacc atgtcataac
    10501 caaaataatc ggttatatac ttctcatatt cacccagttt attattgtaa aaagcacgtg
    10561 atgttaaagt aagaaccttg gcttgatcta ttaatgcttg gataattttg ggatgacaat
    10621 gtccctgatt tactgctgaa tatgcagaaa gaaaatcata atatcggttt ccttccggat
    10681 cccaaacaaa aacaccttcc cctttagcta ttactacagg taggggatga taattatgag
    10741 ctccatactt ctcttccagt tcaattgctt cacgactgct aatatttccg tatttcaaat
    10801 ttgtggacat ttttcctcca tatttatttt attttcttat atcttaatcc cttattaaaa
    10861 aagaaggttt tgtgtcaagg gaagttttta gctgcttggt tatgtgatac acaaaaataa
    10921 atgaaagtat cgtagttata acatcggcaa ttggcattgc aaatattaaa ccattaaact
    10981 ggaatagatg atttaataaa aacagcatcg gaatataaac aaggccctga cgagaaagcg
    11041 aaacaataaa tgctggcaat gcctttccca tagattgaag agttgccatc aaaatcatac
    11101 ccactcctgc aaaaggaata gcaaacacat aggcctccaa aatcattttt ccgattgcta
    11161 taatcgatat atctttaata aatactgcaa taagttcacc aggaaagata gcaaaaaata
    11221 tagtgaaaaa agcagccata gctaaacaga tcaaattggc ggttaatatt atttttttca
    11281 tcctgccata attttgagct ccataattga atccaataag aggttgtaca cctatggata
    11341 aaccaataaa agaaaaaata actatagaaa acacacgcga agcaactccc aaagcagcta
    11401 tagtatgatc tgtataagca gaagcgagat tgaagcttat tgcattccca acactcatca
    11461 taatttggct taaagaagaa ggaataccga tagccaaaat ctctttgtag caagaccatc
    11521 gaggatgtaa gtactgtctg ctgggggcag ctaaactctt cttctttaaa taataagaac
    11581 aaaaatatag catacctagt ccctgtccta tcactgtggc aataccagca ccaacaacat
    11641 ccattttaaa ataaaaaata aacagaggat ccagaactat atttgcacct gtgccaataa
    11701 ataaacctat cattgcatct ttagctgcac cttcagctct gagaaattga attaatgtaa
    11761 atttcatcat aataatagga ctaccaataa aaatcgccgt aagatattgc tgtgcataat
    11821 gagcagttaa cccagtggca gatgttagct taagataggt tggaataaac agtaaaccta
    11881 aagctgcagc gataatagat acaattccta ccgaaaatac ggcggtagtt gttgtttccc
    11941 tggctgtttg aatgtctctt tttcccaata gacgggaaag atagcttgct cctccattag
    12001 caaaaattcc tgcaattgcc attaacatat tatagtaagg caaggcaaca gatatacctg
    12061 ccactttata aggatcattt agttttccta taaaaaaggt atccgttaca ttatataata
    12121 tatttactag catgctcagc atactgggta tggcaagata caatatggaa taaagaacgg
    12181 gagtttcttc caatatttct attttactat actgtttcat caaatgtttt cccttatgat
    12241 aatattttta actatgaaaa tatatctcat gaaagaagta aatacctcct atcatagtac
    12301 atttcaagaa gtgttttata atttaggagg gtggtaatat gctatatacc accctcagtg
    12361 taagttagca ggcatcaatc atagccctgt tatagtatct ataactgatg cagtgtaata
    12421 acttttttaa atagtgtgtt cctccacata attcgtcaag cgagaaaggc aattcacata
    12481 agagccaagt tcgttatcat accacccaaa aattttggca tgcgttacag gtagttccaa
    12541 tattttgtcc gtaaccatac catgagtagc taaagtggaa gctggaatag ttacaaaacc
    12601 tgttctggta tgagtttcat gtgcttcaat aactaccgca gccatagttc ccatcatatc
    12661 tgcagaaaca ttttgccggt tactaactag cagcaggtct ttttgtgagc cctcagcagc
    12721 cttaagatag acatcattaa ttagtttacg attaacaaga ggttctccta attcgtctaa
    12781 gtctgtatga aaagttacat tcaagttgat aagcgaaaca gtgctggttg gaacacgcac
    12841 gctatctgcc ataaaaccaa tatgtttgat attaggcatc acctgctcca gagctttagt
    12901 aacaccagtg gtagaaagaa tgatgttatt caaaacgctt cttgtttttc gtaagtctgt
    12961 agcatccgct ttgggtacac tatccagaat agattgagtg tttgtagctg aatgaatggt
    13021 actcatcgaa gcagttaaaa tacgggaagt ggtttcattt tctaaaagtg gtttcagcat
    13081 atgtgctaaa ccggttgtcg tacaggaagc tgccgagatt aggttatgtt tacgaggatc
    13141 gaaatccaaa tgattaatac cataaatcat agttgtagca tcctcaggaa cagaatttgt
    13201 cgatttcaat ttaaaaggag cactgcaaat tacttttaga gcacctgcat ctaaatgacc
    13261 ccgtaaacaa ggttttccag aatcagtgtg cattgtagga tcaagaaaag tccctgtaca
    13321 atcaactaca attctaaccc cttcattcaa ccaattcaca tctttagggt cacgagctgt
    13381 tctcagaatc ttaactggga tgccttcaat ttccaataaa ggttcctcta catcaagaac
    13441 tttaatttct gcttttcttc cagcaattcc ataaaggaac ttatgaatag aaccataggt
    13501 gctatccatt tctataacct gaataaggtc atcgatggtt ttaccgacaa ctcttccaca
    13561 gtttacaacg ataccatcat aatgtctaag gtgtatctgg ttccataaga gtaacttacc
    13621 aattctacct aatccattga ttcctagcag ttttttgttc ttaaagctca gattcatttt
    13681 gtttttctcc ttatatatgt ttttttattc tataaacaat gaacacttac taaaggataa
    13741 tgtcccaaat atatagctcc actgatagca tcaagagcaa ttttatcacc agcttttaaa
    13801 taagtatctc ctatcctgca attaaagtct aaatcggaaa cgaccagatc acgacaattt
    13861 actactccaa ttaagcccaa ccgtgttgct gtaactgctg catgagaagt tactccacct
    13921 ctggaagtta acaaaccctg acattcaaac aaaagctcca tatcatctgg aacagtatca
    13981 ggacgcacta aaattaaagc ttccccttta tctgcatatt ttttaatgtc ctcttgattg
    14041 aaaacaacaa tgccattaat tgtccctttc ccaatcccta taccataagc aagtttcttc
    14101 atatcccctt tagttgagcc aaaaacctta tagtcaggtg ctttttgaat tacttgattg
    14161 cgggtttgca aaatatatag ttgatcagga gaaggtcctt cgaaggtaaa ttccatttcc
    14221 tgatgggggt aatttttatc ttcaattagc tgcttagcat agcgtaatag ttcattataa
    14281 attgctggaa aagtgttttc tagagaatca gcaaccggtg tttggctatg caaacgctga
    14341 gtttcggaaa taggtaaagt gtgcaccaaa ccagctacaa catcttctcc ttgactgcaa
    14401 agagtaaaat caccatttaa acaaatacct ccttctttac taaagtcata atgagtaaag
    14461 agaactcccg ttcctgactc aagagagata tttcccaaaa tcatttgttg gataattact
    14521 gcagttcccc attcatcggc aatatgcaat ttttgacgga ataatcgtgc tctatctgca
    14581 ttccagctat ctaaaacatt agaaattgcc ttgtagagtt gcataaaagg attttgttcc
    14641 agctcaagat tatgttcgaa aagaacattt ttatagtcct gcaccatttc ctgcatttga
    14701 gcactggaaa actgtgtttt taattcaaca tcataccgtt ttttgtattt aatcattatt
    14761 gcatcgaatt catcacgaag aattccataa gccatacccc agctttggat taagcgtcta
    14821 tagcaatccc aagcggtcca tccataattt ggacgccggg atagtttata ggtaacttca
    14881 tcgttgaggc caatatttaa aaaagtattc atagctccag gtaaactcat tggagctcct
    14941 gaacgtacag aaagtaatag tggtttttct ggatcgccca aaacaaagcc agtattcttt
    15001 tccagaatat tcaaattgtg cataatcaag gaatccagct cattggaaat atcaggatgc
    15061 gtgtttataa tattccggtg tcgataaagg tctgtagtta taacaaaacc tggaggaaca
    15121 ggaaattcat attggtgcat tcgtttcaga aaatatgctt tggaacctaa taacacttgg
    15181 ttttcgatcc gggaattctt tttgttcaag tggaaaaata gcttgtccgg atcataactc
    15241 attactttag gtatatcggc tggtttaaat aaatgtttca tttcggaaag agattccaag
    15301 attcgtgtaa taaaattatc caacccctgc actagaaatg agttagaaag tagattacga
    15361 tagaattctt ctgcaaagat ttccgtttct gtaatcccct gttttctctt atccttagcc
    15421 ttctcagcta tccctaggcc aatattcctt aattgcggat cataaaacct gtaataatat
    15481 tcgttaataa tctccactac actatcttta agcagcttga aaatatccag atattgatct
    15541 atagaaaggt tgcttaaata ctgagcagat cttaacatat ccaaagcaga actatatgca
    15601 tcgttagtaa ccataatttg tttcatcgca taatcgtaaa gttccaggat acgaattatt
    15661 ctacacaagg ttttaccggt tatgtagttt agatttaatt gttgtacatt atgttcgaag
    15721 aggcgtctta ctacattttc gatcctaaag atcataccca ttgcttccaa tttgggttca
    15781 cgatacattc catacataga aggtatgcca gcagcaatgt gtcgcttata atagatattt
    15841 tcccaggcag tagttgtgtg aggatctaat ataacgctat tcatctttcc aatatacgat
    15901 agcaattgcc tgatagaact atcatagtct tttctcataa aagaattagc aaggcgattt
    15961 tgctcgtgag catcgaacca actgtatcga gacagaaatt taacaatgtc ataaggatca
    16021 agattatatt tatccttcag caaaaaatga gaatagcaaa gcagtttaag gcgttttaat
    16081 gaaaaatctt ctggttcacc ttcgaataat ttgttaaccc tttgccagga tagagagagc
    16141 agctcttcag gtataaggtt atgtttcttt aaaaatctgt taactgcagc actttgctct
    16201 ctagtcatct catcgagatt cttcacatac tgtaaaacat cttccggaat catgtcctgc
    16261 agttctctat aatccaaagt tgtccaataa ttcagtattt tctggattaa taaaaggtga
    16321 gtgttattgc tttcagtatg tatctgtttc ctcagaaagt gcattagtag atcttttcta
    16381 tgccctaatt catctatact ggtgctgata tctcttattt caccttcagc tccaatttca
    16441 ttgaaaaaaa caggaaacat tttcagcagg tgtttagtct gcactaatat aggtttaaaa
    16501 tccgaattta aaaatgcaga aacatctttc tggaaaagat cagtatcgga tataaaaacc
    16561 cctttttggc ttaaacttat aatcaaataa gcaagaagat ctttacacat caatggatca
    16621 atagcaatca gctccagcag catacgcaag tgcttgatat gatttttatc aacctcaatc
    16681 tgccaatctt tatctatctt aatcttccca ggaggcgtaa attctagttc cataatctta
    16741 ggtagcagat gctgacagtg ttttttattc ttatgggaaa gcacagtttt gccaatggtt
    16801 aaaatacaat ccaatacaat agataggtga gtatctgtaa attcatataa ggtcttaaaa
    16861 actgaatcca gcattacagg tacttcatct tcattcagtt ctgtttccag gtcagctaat
    16921 tgacgattta aatcccagag cagatgatcg cgcagttctt ccatacctgg taaacccaaa
    16981 aggtaaaaaa tgtactttat tctgagagtt agagttttaa attcacagat agtttcacga
    17041 tgaagaatag caatatctgt gaaggaagga atatcttgta aagattggaa atcctttcta
    17101 tcttctagtt ctttcaacca tttctcgtaa tattttttac ctaaattagc agaaagataa
    17161 tctatatcaa acccattttc agaacttgtc ttttcgcccc aaccttcaat atcagatgtt
    17221 ctttgccaaa attccaaacc ctctagtaaa aggtctttca ttataacgaa gcatatctca
    17281 ttacactcat acacttgcac aagattggat aaacacttgc gaaaagttcc atttatcgga
    17341 atgaacaatt ctttgctcct attttgccag ttattaagtt catccaataa atatgagtat
    17401 acatctttgg gtattccttc tgtgcggcaa agcgcagata tataatccag gtattcaact
    17461 attattctct tttcctgcaa aggtttactg cattttttag cagcaagaag atataaatca
    17521 ccaataacgg ttagggcttg tttactttct ggcaattgaa cataaaacca taaatctcct
    17581 aagatacttt gcctgataag ggttgccacc acttcaggat ttgtataagg atgatacagt
    17641 tcttccaaaa aattccggca tcgttgctta ataccataat taccttctgt taaagaaagt
    17701 aaccatttag cttggtcatc gagcacaatt tcggatactt tggtaactgc aagattttct
    17761 gctaatgcct ttgatttcag ggtttccaag acctaaacct cttaatttta ttgtataaca
    17821 aaacagctat atattctttt tatatttccg ctggattctg tcaaggagtt tttgactttt
    17881 attttttggc taaccgaaac ataaaaaagt gattgactaa ataacataga cattttttat
    17941 ggaaaaaata ataataaatc agagtgaggt tatgatgaga ttggccattg agaaaaaaga
    18001 cctacttgct cacattcaac atcttgtgtc tattgtccct tccaaaaatg taactcctat
    18061 actgaataac tatttgatag aagtttcgga agaaaccaat atgatgcgta tcagtacaac
    18121 agatttaacg cttacagttg tagtagaatt tcctgctgct gtttccgaag gtggcactat
    18181 tgctgtttcg gcacatcatt tcaatgaaat tattaattat atgcccgatg cagttattaa
    18241 tctttggcag catgatgacc tgttaatgat tcaaagcggg aaagtggatt ttaatctact
    18301 gattgctgat cacactctat ttcctgttat tcccgaaccc aagataaatt accctatcac
    18361 tgttgatgct gcattattta accgaatgat ttccaaaact tattttgcag tatcgaccga
    18421 tgtaaatcgg gctattttaa caggtgtctg ttggaaaatt tatccaacct atcatatgat
    18481 ggtagcaact gacggacgta aagtagctga aataaaagta ttgaattctt ccttacttcc
    18541 ttctctggag aataatgata atcagggttt gagcattttt acagaacaga atgaagtata
    18601 tgtagaaaaa gttattccat taaaaaccct gctgttttta caaaagattt tcagtgaaga
    18661 tgtgaaagaa ctgaaagtat ctatggaaca taatcggatt atgtttttat atggagagta
    18721 ctttgtttcc tcgcaagttt ttgaacataa atatcctgaa tatcaaaagg catttccaac
    18781 agaactccct aacaaatttg ttattgataa ggaatctctg ataactgcca tcaagcgagt
    18841 tgcccttatt gccccagatg agaattttcg catacatttt gatatagata acgatcgctt
    18901 tgaggtaaat acaacgaata gagatactgg tgatgcaaag cagaatatgg atgattatgt
    18961 ttacgcagga acctctacag gaatttcctt taactataaa tatatgcttt ctattttaga
    19021 ggcaatcgat acagaaaagg ttgttatcaa acttggctcc tcaaaagatc cgatgatgat
    19081 ttacaacgaa aatcccattc ctgatcagga aattaccttt ctgctaatgc cattacgttc
    19141 ataaaaaatt tgaacctggt taatatcgaa cttgagaatt tccgcaacta tcgtaacagt
    19201 acatatagtg ttggcccctt aggatcatta attattggtc ccaatggttg tggtaaaact
    19261 aatctcctgg aagcgattgc ttattgcagt ataggtaaat ccatccattt tcatcatgat
    19321 gaggaattgc tgcattttgg agagcgtttt tttcgagttc aaggttcatt tattagcgat
    19381 gtgggaaccc cgataaaggt tgctttaggt tatgctgaac agcggaaact attaaagatt
    19441 gacgatttac ccattcgtca gctgagtacg cttttcgaaa ttgtgaagat tatttattgt
    19501 gctccggaag atcatctttt aataactggt tctcctcgct ttcgcagaca gtattttgat
    19561 ctggcaatag ctcagcttta tccgccctat ataactgctt taaggcattt tttacatatg
    19621 gttcaacaac gaaatgctat gctgaaaaaa aattatacaa aagaagaaca aattagttgg
    19681 aatacatctt ttgccacttc tttagccgaa gtttggaatt atcgtaaccg ttatctgaca
    19741 cacattaata ctgccttcaa agaaatcttc aaagatgtct tccccacttc cgcttccatc
    19801 actatatcct atcttccttc cttaaaaatg cctctggaca gttctattga cgacattgta
    19861 cagtatctca gccatttggc agagagagag aaattgttgc aacgctccct agttggagct
    19921 catttagatg attatgaatt cagattggaa ggaaaaaaaa tgcgaagcta tgcttctcaa
    19981 ggtcaaaaac ggatagcagt cattattcta aaacttatac aagcacattt aattgagaaa
    20041 gttacaggta taaagccaat attgctattt gacgatattt ttgccgaact ggatgccttt
    20101 cattcagagg caatccgaaa ctgtatcaat aatcattatc aggtttttat tgcaagtccc
    20161 aaagaagata tttgcctaat atggcaaggt tatccaattc tgcgtctggg agaagagcac
    20221 taaatgaaac tgaaccgtaa tattctgggt tggatgtttt atgattttgc caattctgcg
    20281 tttacaacga tcattgtatc cgtagtgtac agtgtatatt tcataaaaag cgttgttggg
    20341 ggtccttctg gttatggaga aatgctttgg ggaagggcaa ttggattatc aatgacgctg
    20401 gtagctattt cagctcctat tttaggtgca gttgcagatt attcgcgtgc gaaaaagaga
    20461 atgctattta tcaattgcta cctaactgtg atttttacag ccttgcttta ttttgtaaaa
    20521 ccgggtgatg tgtttcttgg tatgcttttt ttcataattg ctaacttcgg tttcaatagt
    20581 gctaatgtgt tttacgatgc ttttttacca gaaatatgcc aacctgaaga cataggcaag
    20641 gtttcaggtt ttggttggtc tttaggttat gtgggaggat tggtttcttt gctcgtagct
    20701 ttagttttgg tagaaatcaa tatccgtttg gtttttccag cggtagcttt gcattttttt
    20761 ctgttttctc tgtttacttt tttctggttg aaagaattca agcgcccttc gaagccaagt
    20821 aattactata gaattgcctg gcagagagtt tctttttctt taaagaacat tgccaagctg
    20881 cctcaacttc taaaatatat tatcagttat tttatttata acgatgggat aacaactgtg
    20941 attgtttttg cctctattta tggagcagaa aggttttcaa tgaccacaca gcaaatgata
    21001 acctatttta ttctggccca atttacatca atagtaggtg ctgctttttt tggttggctt
    21061 actgataaac taaatgtaaa aatatcctta agcattgctc ttatgatctg gattgttgtt
    21121 gttatttggg cattcttttg tggaagcgct accgaatatt attgtgttgg gctggctgca
    21181 ggtttagcca ttggaagcag tcaagctaac agtagaacta tgctttcgct tttaactcct
    21241 cgcgagaggc aagcagagtt ttttggtttt tataccttga caggacgctt atcttcaatt
    21301 attggtccaa ttctatatgg atggatagca cataaaaccg gagatatccg ttatgctatc
    21361 ctatctttga tttttttctt tgtaaccgga tggatcctgt tgcaaagtgt taatttgcag
    21421 gaaggaatta aacaagccga aatgcaataa attaacgaga tatcacatta aagagttaac
    21481 acggaaaatg aaaaggagaa aaagatgaaa aaaaagcaag ctataacttt gatgatcctt
    21541 atgctgctgt tattttttat tggttcttgt agtaaaaata aaccggtttt atatatattt
    21601 aattggagcg attacataga tcctgagctg atcgaggaat ttgaacaaca gaataaatgc
    21661 accataaaat atagtactta cgattccaat gaaaatatgc taaccaaaat tatgagctcc
    21721 cgagaatcat ttgaccttgt tttccccagt ggagatcata taacgataat gcgagattca
    21781 aatttacttg aacctctgga tatcagtaaa atcccaaatt atcaaaatct ggatagcact
    21841 cttttagaga aagcagcaag ttttgacgaa ggcaacaaat atgctatccc ctatttttgg
    21901 gggttaacag gactaatata taacaagcgt tatgttcctg atgaagttat taattcccga
    21961 agttggaata ttctgggcaa tgcttttttt accggaaaaa acaaagttac gatgttggat
    22021 gatgcgcgtg aagttgtagg tgcagcattg atttataacg gatacgatct taatgattca
    22081 agtgtaatgg cattagcagc tgccgaaaaa acccttgccc aatgggataa aaacatcact
    22141 caatttgatt ctgatagcta taaaaatgaa gttccagatg gaactacttg gttagctcaa
    22201 gcatataatg gagatgcttt acaagtaatt gataatagtc ctgatttagg ttttatctta
    22261 cctgtggaag gagcaagttt atggatggat aatatcgcaa tgttaaaatc tagtaataat
    22321 aaagaacttg cctataaatt tatcaatttc ctcttagatg ccgatattgg taaaaggaat
    22381 actgagtatt gtctatatgc cactccgaat aaagcagctg gcaatttact acccgagaca
    22441 ttaaagaata atactctaat ctatccagga gatgaatatc taaacaaatg ttatatgatt
    22501 aatgctcttg gtgaagatgt gaagaaaata gataaactct ttgaggcaat aaaattaaac
    22561 taagaaagtg agaataaaga aacaaaaatg ctcgataatc tgggaaattt aactcgcagc
    22621 cattattgcg gggatttgaa cactgatcat attggacaga atgtaaccgt tatgggttgg
    22681 gtaaataaac gccgtgacct tggtggtttg atatttattg atttacgcga tgttaaaggt
    22741 attttacaaa ttgttattcg tcctgaacaa gaggaaatct ttgccaaagc agaaaaagtt
    22801 agaaatgaat atgtaatagc tgtttgcgga ataattaatg cacgcagtga acaaaacatc
    22861 aatccttcta ttcccactgg gaaaattgag ctggtagcag aggattttta tatcttaaat
    22921 gatgcgcaac ccctacctgt acaatttagt gaagcagcta tggcagaaga agatctacgg
    22981 ctcacatatc gttatctgga tttgcgtcga cccaaactgc aaaatatcat tattactcga
    23041 catcgcatta tgcaaataat acgggatttt ttaaataacg aaggttttta tgaaatagaa
    23101 accccaatcc tgatgaaaag cactcccgaa ggggcaagag attatcttgt ccccagtaga
    23161 gttcaaccag ggaaatttta tgctttgcct caatctcctc agatgtttaa gcagctttta
    23221 atgattgccg gttttgataa atactatcaa attgcgcgtt gttttagaga tgaagattta
    23281 agagccgata ggcaaccaga gtttacgcag ctggatattg agcttagttt tgttacgcag
    23341 gcacaaattt ttgatttact ggaacgctta atgtataagc tctttaaagg tattttagat
    23401 gttagcttaa gcattccttt tccgattatt ccttatgcag aagcgatgga gaattatgga
    23461 tgcgacaaac ctgatatccg ttttggaatg aaactgaatg acatatctac gatcgttgct
    23521 aatagcagtt ttaatgtttt ttcttcagca ctacaaaatg gtggcgtagt aaaagcaatt
    23581 gctattccgg gggcagctaa ttttagtaga aaacagcaag atgaacttat cgaactggct
    23641 aaacatttgg gaggtaaagg aattgctttt gccaaagtta ctgaaaatgg tttggaagcg
    23701 ggtatcagta aatttcttag cttagaggaa actgaagcaa taattgatgt tactggagca
    23761 gtaactaatg accttattgc aattgcagca gataattatg aaatggtatg caaggtatta
    23821 tctggcattc gttattactt agctgaaaaa ttaggtctga ttcctgaaga taactatgca
    23881 ttttgctgga taacagattt cccgctgttt gccaaaaacg cagaaaccgg tgcctgggaa
    23941 cctgctcatc atatgtttac tttaccaaaa gaagaacata ttccctatct tgatctacct
    24001 gaaaaaattg gcgaaattac aggtcagctt tatgatcttg tttgtaatgg agtagaacta
    24061 tccagtggta gcatcagatg tcatcgctat gatattcaaa agaaaatttt tgatatcctc
    24121 ggatttagca ataaagaatt gcaggatcgt tttggattct ttttggaagc gctaaaatat
    24181 ggaacaccac ctcatggagg cattgctcca ggtttggata gattagttat gattatgact
    24241 aaagcagaat ccatccggga tgttattgct tttcctaaat ccttgaaggc cgcggattta
    24301 atgactcagg caccttcgga agttgaacct cagcaatgga aagaactaca tctggcaccc
    24361 acaaagtagc tgttttttgc agcggcttaa gtcgcggttc aaatttgtgt gctatatata
    24421 aatattttgc ggacaataag ttgccgatag aaatagcttt ggttattttt acttgtaaag
    24481 atgcgcctgc gatccaatta gctgaagaaa gaaacttaaa ttaccagata atacaagctc
    24541 gtgatatgca gagttttgaa aaacgagcta tggaactctg ccgccaacat agcattgaac
    24601 ttattgcttt aagcggtttt ttaaaacagc tctctcccaa ttttatagag agtatgcaaa
    24661 tcccgatttt gaacatccat cctgctttac tacccaaata tggaggtaaa ggaatgtatg
    24721 gaatggctgt tcataattct gtgtttgctg ctggagaaaa ggaatcgggt gtaacaattc
    24781 atcttgtcaa ttcccaatat gataacggta agattgtcgc tcaggaaaag gttgatatca
    24841 gtgcctgcaa atctccagag gaaattgccc aaaaagtgtt ggatattgag cacaaacttt
    24901 atgcacgcac catcaaggaa tatcttttaa accattgaaa agaattgcaa taattactct
    24961 tggatgtaaa gccaatcaag ctgaatcctc catcatctta aaccagttcg aaccttttaa
    25021 actggtttct tggcaggaag aagcagatat atatatcatt aatacttgca cagtaacaaa
    25081 ccgaaccgat tataaaagta ggtatctaat tcgtcaggcg ttaaccaaaa aggccaaaaa
    25141 tccttctata caaataatag ttactggttg ttatgctcag cgttatcctg aagaaattac
    25201 caaactggga aatatagatt ttgttgtgga taatcaacaa aaattaaata tagccgatat
    25261 tctggcaggg aatagctatg aatttatgga tattatgcaa gcaaaggact ttgcctgtaa
    25321 actaaccaat aaaatgtatg atcgcacacg tgcctttcag attattcaag atggctgcaa
    25381 ttttaactgt gcgtattgtg cagtccctta tggaagggga agaagtaggt ctgcaacctt
    25441 agaacaaatt ttacaacaag ccaggttatt tgtaaaaaac ggctataagg aaatagtgct
    25501 atcgggaatc aatttagggc tttataaaga tggagataac gatttaaccg atgttttaag
    25561 atgtctgaac gatcttgaag gtctggaatt gattagaatc agttctctgg aaccccaact
    25621 ctttaattat aaactaatta accttcttcc ctctttacct aaattgtgcc cccattttca
    25681 tataccctta caatctggta ccgattctgg gctaaaaagg atgaagcgcc attatacaac
    25741 taccacaatt aagaagttgg tttttgatat tttagataaa attccttctg cagcaatagg
    25801 aatggatgta ataaccggtt tccctgggga aactgaagaa gaacataaat ttacctgtga
    25861 ttttatttgt tctctgccct tggcatatct gcatactttc agttattcta aaagaaaagg
    25921 aacacctgcc gctgaaatga ccaatcaaat cccgaaaaaa attaaaaacc aacacagtaa
    25981 tgaactaatc catatttctc aagagcttac cgctaattat actaatttat tatataataa
    26041 caatatccct ttgcgcggaa ttgtggaaaa tagcgaaaat ggttgtagcg attttttaag
    26101 cgatcactat gttcgcgtct atttatctaa ggaatatccg gcgggagaat tagtaaaaac
    26161 ttcagctaaa gaagttaaaa ttgctgggga atcagatgtt taatgtgatt agtaaaaaaa
    26221 tagaacacag ataacgggat cgacaggatc agctggattt tagttgagcg ataagtaaaa
    26281 ataagaaacc tgagtacttt tttaatctct ttcggcgttt ttagtggctt ttattatctt
    26341 tatgctttat atttgtacaa tctatgaaag cttattttat aggttctaag tactcatcta
    26401 tagtttgata tttccacttc agtaaaattc tatccaataa agcaaccatt ccaaataatg
    26461 caatactaac agcgacgata actataatta cagcaaaaac atgagctgtc tgataggatt
    26521 ttgtagctct ggtcatatat attcccaagc cggaagttcc tcctaaccat tcaccaataa
    26581 cagctcccat aacactataa gttgcagcca gttttaatcc tgtaaaaaag ttaggtaaag
    26641 cagccggaat ctttaataac ttgaatatct tgtatggtgt tgctcccatg gattttaaaa
    26701 gacgaatctg atctacagaa acacttctga aaccgtcata aagtcctaaa gcaataggga
    26761 aaaaacacat aagtacaacg gcaaaaattt tggaactgat tccataacca aaccagataa
    26821 ttaaaagcgg tgccacagct attattggaa ctgtctgtga aattatcaga tagggataga
    26881 gaatttgttt tatccaactt gataagttca ttgccatagc cgttatcgaa cctaatatga
    26941 tactgatgat aagtccggtt atcgcttcca gcaatgttgg ttttaaatga tgccagataa
    27001 gccctggatt tttcataaaa acatttatta cttgtaaggg cgaaggtacc acccaaaaag
    27061 caattacacc tttcgcactg ataaactgcc agagtataat caggaaaata aaaaaaagca
    27121 aaggaagtat tactttttgg cttttcacat ctcgcccaga aagcggattt cagtttgcag
    27181 cttaacatta taatgttcat acacaacttc ttgaatatgc tgaataagct gtaaaacatc
    27241 ttttgcagtt gcagaaccca gattaacaat aaaaccacaa tgtttttcgg aaatagcagc
    27301 atctcctatt ctgaaacctt taagcccaca atcttcaatt aacctgcctg tataaaatcc
    27361 ttccggtctt ctaaatacac tgccagcact gggtaattca agtggttgtt tttgctgtct
    27421 tttttgttga tattcatcca tccgccgttt gattgtttca gagttatctc tttttaaatg
    27481 aaacacagaa gaaaggtgaa ttaactcttt atcttgcaac acactatgac ggtaggaaaa
    27541 attatgctct tccactttta gatataaaat cggacttaat gaatttatac tttcccaagt
    27601 aggtaatata catacgctgc tatgtagaac atcttttatt tcataattat aggcaccggc
    27661 attcataaaa acggctccac ctactgaacc gggaatgcca gaggcaaact ctaatccgct
    27721 taagccattc tcacaggcat aattacaaag atcttttaaa gtaactccac agcttgcagt
    27781 tatatattct tgttctacag ttagttctgt aaaacgctcg gtgctgatca caattcctga
    27841 aagtccctta tcggaaatga gcagattaga acccttacct aagataaaat agggagtttt
    27901 atgattaaga caaaatctta ataaatccag caattgattc tgtgtgtaag gagtgcagaa
    27961 aacctcagct gggcctccaa tttggaaact gcaatgttct tttaagggaa cattgctttg
    28021 gataattccg tcttccatta atttaggata ctcttgctgt aaaatagata aagttgtaat
    28081 attcatagat tactcggtag cgagctaaaa aatttgccaa tttcggatat gagttgttgc
    28141 tcatttttta gctcaataca gcgacctggc aaaatttgtt taaagtactg gccgtatttt
    28201 ttcttggaaa cgcgggaatc cataatcagg acaattccgc gatcggtttt acttcttatt
    28261 aggcgtccaa aaccttgtct taaacgcaat agagcattgg gtaacattaa atgcataaag
    28321 gaatccttat tctcccgttc cagtttatct attaatgctt ctacgagagg ttctgaaggc
    28381 acttgaaaag gtattttata taatatcaaa agagaaagtg aatcgccttg aatatcaact
    28441 ccttcccaaa aagaagaagt tccaagaagt acagcatttt tacttttctt aaaatcatcc
    28501 aagatagaat ttctgctacc accttttccc tgagcaaaaa aaggacgctt agaatgatag
    28561 agggtatcgc taaggtgttc gtaaacagca tccaaatctt tatatgaagt aaaaagagcc
    28621 atcgttccca cattggtagt agttaaaatt tgttccaaac aacccaatgc ttgactttgg
    28681 aaaaatctgt ccttgtgttc aggtagaaaa ctaccaatta ataataagga ttgtttatcg
    28741 taatcaaaag gagattccac aatgctctcg atcactttct tgtcggtagc aaaacttaag
    28801 cctgattgat ttaggaaaaa cttgaatgaa cctctaattg acaaagttgc cgaggtaaaa
    28861 actatgcaag gaatattttt gtaaagtaaa cggttaagcg gttctgagac ttctactggg
    28921 gcataacaaa aagtactgga agggatatta cgttcgggac gcggaatatt ttctatccat
    28981 aaagcataat tatccagatc aggattaagt aattccagca ataaagtttc cgtttcccga
    29041 tagcgttgca aataaccatt caaattatct ttaataatct ccaaattagc tatctggtta
    29101 cttttcacgg tacgcaacac attatcaaga gcaaccaagt ctttaagaat atttttgaaa
    29161 cctatggaaa gttctttcaa gaaaaaatag atatcctgaa aatcagctat gtcttttatc
    29221 cttagtttat tataactttt cgcttcaaag cagcgattcg ctgctaaatt aaataaggaa
    29281 gtaatcactt ttcgttgctc gtctgtctcc agcatcagct ttgtgcaaag agattctata
    29341 tgagttttct ggtctgtagt taaaatactc ttatgcactg tttgaataag ctgaggcaaa
    29401 aaaccaccgg tatatttttt cccagaatga gctagctgat tgaataaact gttaagctca
    29461 gcataactga aagtaaagcc caaatttttg gacgcagcag acataaggtt atgtgcttca
    29521 tcaattatta aatattgata ttcacctaaa gtggaattat ccatttgggc atctgctaat
    29581 aatagagaat gattggcaac tactatggaa gcagattcta tatgcttacg caacttcatt
    29641 acataacaat tatgataatt aagacacttt cttcccccgc aaagataacg atcggaacat
    29701 attttatgcc ataaaacact atagtaattt ttatcgaatg aggaattttc ggaaatatca
    29761 ccggtatgag taagctcctt ccaaacaagc aaatttaata aacctcgggc atcataagct
    29821 gatattcccc gagtttgctc ttgtaaaaga tcttgccaac gtctttcaca aatataattt
    29881 tctctccctt taactaatac tgctttaaat ggaagaggga taattttacg caattgaggt
    29941 aaatccttga agaaaagctg ttcctgtaaa ttttttgtgt tcgtagaaac aattactttg
    30001 gtcttgttcc tattgctaaa atctatcgcc ggaactaaat atgcaaatga ttttccaact
    30061 cctgttccag cttcaacaac taaaaaatcc tgtttagcaa aggcattagc tacctgttca
    30121 gccataatca tttgtccgga acgaaattcg aaattaggaa atttattagc aaaaagtccg
    30181 tgttctgcaa atatttctgg aatgctatct atagacccag agacattatg ttcaattaca
    30241 ttgtttaatt cattcttggg aggaatgggt tttttccctg ataaaacagt ggcacgctgt
    30301 tgttttacta gccatctaag atattcagtt aaaccacttt ctacatttgc ctgtagactt
    30361 aaatcctgca aacgggcatt aagcatatag ttaaagtgtg ccaaagcata attggtaaga
    30421 gcattaaaaa gatatccggt agctaaagca tcagaatcag cacgatgagc attatccaat
    30481 ttaataccaa aaaaagaaac catagtactt aatttatggt cagtagtaaa gggtaaatac
    30541 atcctcgcta tttccatagt atcccaaaac acattaatta atggaaaact tccattttct
    30601 actaaagatt ggttgataaa ggataggtca aaattgatat tgtgtccaac taaaggagtg
    30661 tttccaataa aatcgcaaaa ttccttaagt acctctttta atggaggaga taattttaat
    30721 tcctcagggg aaatatgagt taattgttca ataaatttag gaaccttgcc tttgggtttt
    30781 acaaagctaa catagctatc aacaacttca ttgcctttaa aacggaaagc tgctatttgg
    30841 attatttcat ctcgcgctgg cattaaacct gtggtttcaa tatctattgc cacaaattct
    30901 aattgcgaga gaagcagttc ttcgcttaca ttttctgagt ttaaatcttt accgttttcg
    30961 ctttccacta tctttttccc gtaagttaat cttcttcctt atagaggtta tatcgttgca
    31021 tcttttttat cagcccctga cgagatagcc ctaattcttt tgcggcttga gtttgattcc
    31081 attgaaattt ctctagggat tgagttatta ttttccgttc cagttcttct atggcttctt
    31141 tcaatgattt tttacctgac aacatagcga ttgttttact atattcaatg tgatgaaata
    31201 cttcctcact aaaatcactt ggtttaataa aagtatttgg ttcaacaagg gttactgctc
    31261 gttcaatttc attttccaac tgacgcacat tcccaggcca atcgaattgt tctaaaatat
    31321 ccattgcctc cttagtaaag cccagaacat ttttatccat ccgttttgtg tatttatcta
    31381 agaaatgaat ggccagtaat ggtatatcac cagtcctgtt tttaagttga ggtacatcaa
    31441 tccggataac atttaaacgg taatataggt ccaaacgaaa atcccctcgt tttacccttt
    31501 ccattaaagg aacattcgtt gcgcaaacta ctcgtacatt aactttttcc gtttttgtag
    31561 cgccaactct tttaacttca ccctcctgta aaaatcgaag caattttgct tgagtagatt
    31621 gacttatatc ggcaatttca tctaagaaaa aagttcctcc atcggcaatt tcaaagaggc
    31681 cctttttatc ataagcagca cctgtaaaag aacctttcac atgaccgaat agttcgcttt
    31741 ctaataaagt ttctggtaaa gcaccacaat attgagctac aaatgctttg tttcttcgat
    31801 tgctgctata atgtaaagca cgagctatta gttcttttcc tgttccactt ggtccttcca
    31861 aaagaactgt tgtaggagtg tccttcactt tttcaataat ctcaaatatt ttcatcattt
    31921 cagggctttt accaattata tcagcatata tattattgga agtgagctgt tctcgaatta
    31981 ttgcatggct tttttccaga ttggcactac gaatattatc aatgattaca ataatctggt
    32041 tggcaagggc agagagaaga ttaatgattc tttccgagaa atacttggaa ccgcttttgc
    32101 aaaaaatgag tattaccccc atagtatttt tacggatgga tagaggaagc accaaaatat
    32161 tattataact gggggcaaag tgaggttgct taatattttc cagtttattg ttctgatata
    32221 catcagcgca tatatttaag aaatgatcgt gatatttatc gtctggcgaa aaattgttat
    32281 aaattttata ctcccaagca tcaggcaaat tgggattcgc atgaagtaac agaactccac
    32341 catctgcatt ggcaatttca atcaagctgg taagagattg ctcaattagc acatccaaat
    32401 cagttattgt attcaacttt tgagtaattt cataaaattg gttcagcagg ttttcttcaa
    32461 acttggcttc ttccatttgc gaagcatatt cgcgcgaaat tttttgcatt agaatgtcgt
    32521 tctgttccag cagttttatg gagccatatt tctctattat cttcctatta tttttcaaaa
    32581 tctgaagtgc ctgctcccat tcttccatat ccaaaagaac tccagcaagt tcataattaa
    32641 ctaacgatag ttcatagttg ctaccaactt ctacaaatag cttaattgct tcattaagat
    32701 agttttttgc ggcttctaaa tttttccttt ccagcacagc acgtagataa taagctctac
    32761 ctatttcgaa aggcaaattt tgttctgcag ccagtttctg agcttcagaa agataataat
    32821 cggcactttc cacattgtga gttaaaataa agtaatatgc ttgttttaga tagccctcga
    32881 ttattttgtc acgagcagca atattcttga agaaatccaa actctctact aaatatctat
    32941 aagcctcttt aaatttatgc agcttagtta aaacactacc taaattccca tatagctctg
    33001 ccttaatata atcatctgat acagcaggta acaattccaa gcctttaata tagagatcat
    33061 aagcaagatt taattcatcc tgtttttcgt agacctcacc cagattgttg aaacttctta
    33121 agaggccttc attaaaaccg ctttcctgtg agagcttaat cgctttttca tagtaagata
    33181 tcgattcagc ccagtctcct tttttaaaat acaagcctcc caaattattt aaagaagcaa
    33241 ttgtattccg ttgaaaacca aattcgtttg ctatctccaa agattttttt aaagcatctt
    33301 ctgcgcgatc atattcgttg tgatccatca gtgtgacacc aatattatta tagatattag
    33361 ttatattata tggttcggca agtaatcttt gaatttttag cgcttcttcc aaataatcga
    33421 gagatttttc aggatcacct ttgtcgtcca ttaagccacc cagattattg tagcatacag
    33481 aaattccatt cagattgtaa tattgctttt ccagtaccaa acttttcaag aaataggcct
    33541 cgctcttttc ccaaaagccc tgaaacatat ataatacacc caaattatta tatatcgcag
    33601 ctaaaccact gtgatcttta gcaagagaat atagagtttc cgcttcatta aaaacttctt
    33661 ctgcctgctc ccattcattt atataataat gaatcttacc acggattttt ttggcttcag
    33721 cttgtacgct atgtctttgt tgttgattag taatattgct aaaagaatga ctggcattat
    33781 ctaaagtgtt aaaagcatca ttaaatgcct cactttctgc ctggatatca gatttcagaa
    33841 gtagtatttt cactttccaa tagggatcta tcgtagctgg agaatattgt ttaataattt
    33901 ccaaggcaaa agctgaagaa ttaatcgcat aaagatcgtt tgctaattta tagactatct
    33961 gttcagccgg gaaattattc tctgtgcaga tatgcaatgc ttgccgaaaa taatctaccg
    34021 ctttatcttt ttgatttata gctgaaaatg cttctgctat cttttgtgtg cttgttaatt
    34081 tgccagtggg gtttttagta tgcgctaata tatactcttc caaggttaaa acactttcaa
    34141 aatcattaat caaagtaagc tcggatacta tcttggcaaa aagtgccgcg ttatatttat
    34201 tattcataat ctgcagagaa gccaaaattt gaggatatct cttatccttc tgaagatatt
    34261 ctatcagctg attgaaaata gtgctaccaa tttgatcttt attatcgttt aaccattgag
    34321 aaaatgcttt tttcttttgt attgaacaaa ctgttccagg attggaaatc aaacgcaaat
    34381 catataataa tgtcagatct gctttcaaat cgtctggttt gctaagcgtt tgataaagtt
    34441 catctgtatc taacccatct aatatataca taactgtcaa aatctctttt tgtgagggac
    34501 taagcgattc taacatttca tgatatattt gttgagcatc aaaggtcttc tctaaataag
    34561 ggccaagatt aaacttattc ctggtttttt gtgcttgcat ctctcttaag accttttcga
    34621 ttattatcaa attaccagag gttattttct gaaatatttc actatcacta ctattagaaa
    34681 aatcagttcc aggaaaagtc aattgtaaaa gccgttgaca atcctcaatc tctaatgctg
    34741 gaatgttctc catttcagaa aaaggaaata accgcgtttg cgtgaaagct acaatctgaa
    34801 taccagtttc tggaagatat tgaaccaaat attgtaaata atcacgcgta taagcatcta
    34861 aaacatcaca atcatctata acaagcaata atgaatgaaa taattccatc ccatttaatc
    34921 gttctgttat ataatagaaa aagtcatatt tactatccga cccaatatta tcccgaaatt
    34981 cattataata accgtatagg tcagcttcac tcacttccgt aattacctga ataagctcag
    35041 taaaatgatt tagtgccaaa ggatgaggtg aaaagcacat cctcttctca taaacatcag
    35101 atacactatc cattaaaggt tgaacataat gacttttacc ggacccagat ttacctgtaa
    35161 gctcaattaa atgactctta gaactttcac gcaagtaacc catagctctg gaaagcgcta
    35221 ttttatttat aatatatgca tccagctgtt gggttatatt ctctgccatt tttcacccct
    35281 ttggagattt tgtttacagt gctactgtaa atagagttga caacctgtca agtaaaaaat
    35341 gtaattcaga tgttaccccc ttttttagcc cgtagggcga cacaataata gcgacggtgg
    35401 tgtcagcctc tcgaaataat aattttttta ttatcaaatc atttgctttt cttttaattt
    35461 atccggatcc caatgctttt tcaaaagtgc atcaaattct tcttcggttg ttatattgtg
    35521 atgatgatct tcctgatgtt ctatgtaatt tttcacactt tcaatgttgg atttgctgac
    35581 agaataggca gcatatccat cttgccaatg aaaatcttgt ctggtctgtt ctctgaacca
    35641 tttactggaa ttcgctttta attttgccat aaattccact atactcattt ctttgggggc
    35701 taaacatagg atatggatat gatcttcaat tcctccgatg gcaagaggaa taccatttaa
    35761 atttttaatt attccgttta gatagccata catttcatca gcaaggggtt ttttaagcca
    35821 ggtcttccgg ttttttgtag aaaacacaat atgaaccgct aaatgacaaa aagatgcagg
    35881 catattttct ccttgtttga tcttattaat tcattatctt ataattgaga ttttatgtca
    35941 agagaagaaa gttggataat cattttcaca cgaggggctt acgccaccgt cgctatcatt
    36001 gtgtcgccct tgggggcttt ttgcaaaggg caaaagagca gagggcttag agatttttaa
    36061 atcccttttc cgaggggctt acaccaccat cgctatgatt gtgtcgccct tgggggcttt
    36121 tttcagaaaa catagttata aaatgtaatc aggtttcttc ctttttacga ggggcttacg
    36181 ccaccatcgc tatgattgtg tcgcccttgg gggctttaaa agtatcaacc ttataaactc
    36241 tataaacttt ctcaaccctc taaaccaact caaccctctc annnnnnnnn nnnnnnnnnn
    36301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    36361 nnnnnnnnnn nnnnnnnnnn naaccttata aactctataa actttctcaa ccctctaaac
    36421 caactcaacc ctctcaaccc tctaaacctt tttttaagga agcaatatat gaaagaatag
    36481 gatcaaatct ctttatccag atattgatag ccaggaactt ttgttagcaa gccgtcttcc
    36541 actgctgttt ttaccgaaag gcagggtaac tgttgttcca gaatttgctc tttagtaata
    36601 gtcttcaggt tcacttgttc ggggtatttg gcttgcattg cccacagcat ttttacaaaa
    36661 agtgaatacc aagagggcac ccgaacgcta tcatccttat ctctcaaaag gttcaggtaa
    36721 tttttaaaat cgctacgata cgcttccgag ggattgatag ctttaatttg tttggctatg
    36781 gcagcatatt ccagattttt ttcctggatt ggcatttgcg gagctcgccg cattaaagtc
    36841 cttttcaagc ggggatgata ataatatatt gcaccatcca gtttacctga aaatcccttt
    36901 attccatctt ttacagttac tttcattgta acctccctat attttgttgc caaattattt
    36961 ccttgcttga aactgtcaat cacttcttta tcatccctta atcactcctt aattgttaac
    37021 gcttgattaa gggatgataa aggagtaata aacttcccga aataagggat aatatggttg
    37081 caagtggtca ctgaaaactg caaatggcat tggggggcta aattctaatt actaatactt
    37141 gagtgctcta tgaacccatc tgatagacgg ctatatcata gcttagttga taagtacccg
    37201 ggttcaagtt gccaaggttg aagaagatag cttactttgg atagtgattt tgataaacta
    37261 tacaatgcta aaatcccagc tgaattttta ggtagaagtt cgatgcatct gtaattagat
    37321 cattatctga ttgaatggag taatgtgagt attggctttt gtaacctgca tatatgtgac
    37381 tgtttgaacc aacattccaa tctaagttaa ggtagtatcc cagatttccc aaatattcca
    37441 tatccggctt catagttatg ttgtagtttg tcaaactcag tccctgactg aggattatat
    37501 ttttttgtaa tacaatgttg tttattaggt tcatcagatt gtattccgga tcccaataat
    37561 caagtttaga atgtgagtac cgcacatagg taagattata gtatatccat aatttttcta
    37621 gtaaatttat ctgaattact ccgtaataat ttatgacagg taaaatttca gagagggaat
    37681 aaactatttc cttcccaccc gaaacggatg caaaagcagt caaacgacta ttttttagcc
    37741 aatttaaacc aagggtggct ttatagggat gatacaattg gctttggaat ttttcggctc
    37801 tataatatgt ttccatagca attgcatagt tattattatc taaatacact aactgagaat
    37861 agtttccaga agaactcatt agttctccgc tgcttaaggt attatccaat tgccattgca
    37921 catttgattg tagccgagat agaaatccgt ttcgaggttt atcataaaaa aagccaaccg
    37981 tgttttggaa tctatcttga ttgtaaaagg atcccatatc tgcgcgaaaa tttgtatcag
    38041 tcagttcaga agcaacccaa aaagtataat cagaatgggt attggtaaga gtgatacctg
    38101 catcatagcc catagaggat aatgaatctt gtttcatata gctgaaattc acaatgggta
    38161 agatctccaa tggctctaaa ggacgatagc taaatgaagc atgcgctagt tcattatgga
    38221 aatcgtcatt ctgcctgcca tataaattga tatttacttt tgaggtagca aaggttctgc
    38281 tgtaaccaag agcattaaag aaatcaccgt tagatagatt attgcttgga tcgtctctta
    38341 gaaacacaag agccacagcc cgatcctgag atacccgagt atatttgcct gcaaaaatgg
    38401 gacttataat gtttcgtgtg taccaaagat ccgacgatac tgccaataaa tcataatcct
    38461 ccaagaaaaa ggatctgttt tcagctattt gaggagggtt tttgctatta tagttgtcaa
    38521 cagcagaatc taagggtgta tcagagaaat ccggctttat ggatatcttg aaactagcat
    38581 tagaatatgg ttttatgttt aaattgccac ctatattttt gtaccagatg tttcctctgt
    38641 tttctttact atcatatatt gccacataat aggcatcagc tttaaaattc ccttgcttgc
    38701 ctattggatt gtttataata atctgagcac cgttttgaaa gaaaaaggga ccgcttgtat
    38761 attgaacata gggataacga tagctttttt tgccctggta gcaaaaacga cttacataca
    38821 ctcccaatgt ataaggtgcc tttgatccca tccgtaagtc acaccagggt attcttaatt
    38881 ctacaagcca attattgtca ctaagtgtag atgttaacct ggaatcactg ttccaactcc
    38941 aatctgcatt gaatgaggtg tctaaggtat agtcacttaa tactcctaaa ggagatgcct
    39001 caaaaccata agcaaagaat tggtttggct gagtgatcaa ggtaaaagct atcgcgtcac
    39061 tttgggtatc tccatccttt ttaacaattc tcccccgttc caaagaacta tctacttgag
    39121 ctatgtatat gcaatacaaa tattcttcat cagcataaaa cataactgaa tctgcaaaag
    39181 gagatgggga aaattgattg tccttatctc ctaaaactcc gatatcggat gagatagggg
    39241 ttaaatcttg gtatttttcc tgaatatttc taagcagttc ttgagtttca atttgctgag
    39301 gataatctcc aatttgtgta ctatatgctt tgggcaccaa aagcattgtg aaggcaatga
    39361 aaccaaggat aatgttaatc tttactacac tcacttgttc tcccaatatc tgtttctgat
    39421 taatcagtga gactaaatta tatctatcgg tcttctaaac tcaagaattt ttcttactgt
    39481 aacatagaaa tttttatcaa
//
LOCUS       DAOSTH010000011        37346 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_1236_length_37346_cov_14.732563, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000011 DAOSTH010000000
VERSION     DAOSTH010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 37346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37346
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1236_length_37346_cov_14.732563"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..558)
                     /locus_tag="PLB65_03075"
     CDS             complement(118..558)
                     /locus_tag="PLB65_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOQ77539.1"
                     /translation="MRTKIFTNLVGLHREMLKLIGEVSALTDSPLALEDAIDDVWHPK
                     CDVFQTDTQWVVIAEMAGIEKEDINISISPEYIRISGTRLFPNCNSILCYYNMEIETG
                     KFDRRIFFPDISIDKENPKISYINGILRIAFNLAPVVERIIPVQ"
     gene            complement(580..1911)
                     /locus_tag="PLB65_03080"
     CDS             complement(580..1911)
                     /locus_tag="PLB65_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOQ77540.1"
                     /translation="MKNILISTILLMLTIPLLAEDWLTIYNNDLSLVRNRFELELVEG
                     RQDINYSDITSRIDPASVIVTGNGIRIAEQNYEYDLAGKWQIMAKYLDREVLVITKDQ
                     SRLTGILKFYSDNSIGIIEKGTDRLLVISESETQWIQLAELPENFYTKPTLHWNIIAS
                     KKGKHPVQMSYLTGGFSWDVTYNTVWDEKTLQFNSWVTINNTSGKAFNDVNLKLIAGD
                     INQVYSGYYKERKGLISYTAGMAQEELAPSFDEKAFHDFHMYTLDQKVSFANNQTKQL
                     ELYPSKNIKAYSEYEYPTYATKIKSMIKFKNTAENGAGMPLPKGIIKVYKQDSDGNLE
                     FIGEDSINHTSKNEEVSINTGTPFDLIASTRLKEQTQISKYITESLIQVTLRNNSDTN
                     KTIKVTHQLSPNTRIVEADYKYTKDNNDKVTFSIDVAPDKEIVWTFRQRSE"
     assembly_gap    2205..2304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2478..3029
                     /locus_tag="PLB65_03085"
     CDS             2478..3029
                     /locus_tag="PLB65_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77541.1"
                     /translation="MKKVLGLLIIVLFLMSCSDEPTKVTVRDDNQDKVAHLGPDPNLL
                     GNTEIFFIPGTIQGSAIWIINGPGANVGIDIRDRENSSFVYYADSYIGSGSNSAQTGT
                     QIPWNKWMRVRLAVYKSGLSGIIVNFIQALGLDFFDSLESYMIEQIYENDVYLSGNGT
                     QIKTPVKCYNDISKVTGGSIQVH"
     gene            3213..3503
                     /locus_tag="PLB65_03090"
     CDS             3213..3503
                     /locus_tag="PLB65_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77542.1"
                     /translation="MTYYYESPSATTTKHIDEEPLVPSMKVSEWLITLLLTCIPVVNI
                     IMLIIWSLDTKGNPNRRNFAIAVLIFMAAMLVLSGFYIGQITGWFFKFLNLM"
     gene            3519..4961
                     /locus_tag="PLB65_03095"
     CDS             3519..4961
                     /locus_tag="PLB65_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HOQ77543.1"
                     /translation="MNYLDLAMNAAETLGAEYADIRIQKTIDQVIYLQNLSLKHTSND
                     VQQGYGIRVFKNGAWGFAHNNVFSDEAVLATVKKAYETAILSAEVNKDKKLSLAPERS
                     YLATYKTPIKIDPFNVPLTEKIDLLLEVNRVMLAYEGIKQARSLLIMHKDEKLFANTL
                     GTRLDLSTHFINPVITAVAVTEDDSQSRTFDEGGRAIGWEWILELDLINIAKQIAEEA
                     LIKVKADTLGEEQRRALILDPNHLGLTMHESVGHATELDRVLGWEADYAGVSFATPEK
                     LNNYQYGSELINFVGDNTLEGGLATLGFDDDGVPGQKWYIIKDGILKEYGSTRDTALE
                     IGLNSSRGCNRATYYFNQPINRIPNLYLLPGTKPLTPGELIADTEDGVYIQGRGSYSI
                     DHHRINFQFGGDFFWEIKNGKLLRPLKKVIYKSYNPEFWNSCDAICDERFWRPFGVVN
                     CGKGQPSQTARMTHGSAPARFRNIRVGGSQ"
     gene            4958..6232
                     /locus_tag="PLB65_03100"
     CDS             4958..6232
                     /locus_tag="PLB65_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HOQ77544.1"
                     /translation="MNSEKIVKYIQTNCKADDWSLNISEDDSHETRFAQNSITQHISG
                     ATKEISLSVSFDGRKGSCSVNQDNEESLAYLLKTAEDIAKLAPVDPEFVPSEGKKDIP
                     EVDNCDPETKALQPKQLVDIVQTSINEAKKFGATVSGLTEKHIRSDGLFTKNGFAGEF
                     EYSDFGHSMTLKKEEVETKVSYEAKKFADFNLKDLCDKLQHQATTLAVKQNFEPQKIA
                     VILRPVALQELLWFMGWMMDRRQSDEGFTPFTDKIGKPFFGDKFSWYSTMQQPQLLAL
                     PYSSDGIISEEIPWIEKGILKNLPVSRYWAKKINSKPSSAYNMYIPGEGYTEEEMMKM
                     VPRGLIVNTFWYIRSVDTKAGEFTGTTRDGVWYFEDGKIKYAVNNLRFNEIPHDATRR
                     IISTGKSELASPSSLLPAMLIDNFNFVDRTTF"
     gene            6373..6546
                     /locus_tag="PLB65_03105"
     CDS             6373..6546
                     /locus_tag="PLB65_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HOQ77545.1"
                     /translation="MPDRTLICQDCSKEFVFTEGEQEFYKEKGLQNEPKRCPDCRKAK
                     KAQFNRNRRRQSY"
     gene            complement(6858..8855)
                     /locus_tag="PLB65_03110"
     CDS             complement(6858..8855)
                     /locus_tag="PLB65_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77546.1"
                     /translation="MLKYTLIVLFSLLISGFLNYQTNPEIEKKRRITLFVLRFFSISL
                     LLILLLSPILYYTQHKKQTAQILVLEDVSQSMNLKHNSGSKAQHLKPLWQKMEKKFAD
                     NDYEVIHKTFANGLNGDKNATLLAPALKELSNQKAFRNLEGVVLASDGWLRDESLSIV
                     QQLGCPFYVLADSTVNPTPDLAIVNVRYNRYAYKNEPNTIRAEISSQNYSGNAEVRLL
                     LANKIISRQNMKLEKGIISTVDFTQRFNNIGFFPWKVEVVPLPNESQINNNIFPGAIE
                     VLSEKQRIILISDKPAWDNKFTLDAIATNSRWEAESYLNRNGRLFSGEVLVNELDSHN
                     LAAIIIINNGMLNLDNSTANFIQNAFQRGVGIMYQGLPVNELSAILPIQKSNITSIYQ
                     GFLVPTAAAANYPMLNSLTVNTKDIPPLDYYYVTASPGTDILATINNPQNSPAIVVQH
                     TKTARSIAFATLNLWRWQMQSSEEGYTKLISSCLTWLSNKSTGNYTAIYNNSYFQGEE
                     IRIRLRNENEIRQSRLNVNPRIRIMNKNNKEVYTDFLKREGEEYAISLQLSEPGTYSF
                     EISDKESGNSTKGKFELSETSIETRDYGYNLPLLSWLANETGGKVLYASNIDSYNPTP
                     AKPIEQITRKEIPLYKKWYVIALFIFTFCLELFFRRRWGLL"
     gene            complement(8855..10105)
                     /locus_tag="PLB65_03115"
     CDS             complement(8855..10105)
                     /locus_tag="PLB65_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HOQ77547.1"
                     /translation="MKRCILLVLIFLISVSVLNAFNTGKSVLFADSYMLRAKGVEAAY
                     WNPANLKQVKGIDFWLPIMNLGISVNNNSLDLDTYNYVVSQDFLDDEDKIKILNKIDG
                     SLRSAVEGNISIIGFAFNNISFSSSLHLTGRLAVSERFVDLLLYGNTDSLYVFDKSTT
                     DISGLSYADFTFGMGNFIIPFIPEPIPKIRAGFSVSALVGVGDANTQEFNGYFNSSME
                     GLSLQQDISLHTGIGGYGQKVMLGLASNVTPQLEVGLTLDNLFGKITWPFTTEARNIS
                     ITADDVYASNIDEEFYDYTSETVDIDTYSTELPPELRLGALYSMKRANLSVDYLQGFK
                     SSINNSNIGRISMAAQVLPLKFLPIGMGVCFGNSIYPWRVSYSIGLHTSTGEIGFGVQ
                     SIKQLFPSYKSKGLSIGSYIKVNI"
     gene            complement(10107..11612)
                     /locus_tag="PLB65_03120"
     CDS             complement(10107..11612)
                     /locus_tag="PLB65_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77548.1"
                     /translation="MKKYYFILITALVFLGACEVKKPTMPSWSVKLNIPLINDFFYVS
                     DLVDSVNIVLNEDNILTVTANGEATTNEFGNLPFNPNINLTGIPLIGSSYDFYIPFTD
                     PTGTVEIAYAELAEGVFESRLRNVDPNVDQISLVFHDIYLPNSNQNLVIISDNFDNWV
                     TTNLDGMHLGTPNTNEIITQLHISIQVNSTLPAGTQVATLDFRSDSMISLGEFRGGLH
                     NYPVNLNDSDSSINIDYPHGLDQTLTLEEASLQLTLQNYIGFGAEFSGQLKATNAAGV
                     SRIIDILDENGNNFQIQPALQDGPVVMNFNLYDNITELLQLMPTTIQVIGGCFEIESD
                     NNIGSVKPTDTMHLDYLINAPLIFTLHEHEIVINEEHEFSIPEDNRERIQKNALSASL
                     NMQIQNMLPIGASAKLFLSNTPNIDIHNPGTYSFLKQVEIHSFNQNPGFQDVELELTK
                     DELDLFASEQVYMRWSFSFEETGSPVTIHASSGDYIHLKGMISAEILIEED"
     gene            complement(12133..12912)
                     /gene="rnc"
                     /locus_tag="PLB65_03125"
     CDS             complement(12133..12912)
                     /gene="rnc"
                     /locus_tag="PLB65_03125"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOQ77549.1"
                     /translation="MEKRTKSIISRIVEYFNTKKIGEKYPKWEKSLTQLQKKIDYTFH
                     DPTLLHAALTHKSYLHRHYSEHKTPSPFERMEFLGDSILGFIVSKELFTEHPEEQEGK
                     LSKLKSKIVSETYLTFKANAIDIGKYVLLSPEEQQAGGASKPSILSDTMEALICAIYL
                     DSGISAASKFIRRFILDNYQETVNREELVNYKSILQEYMQSRNQGPPNYVTIAEEGPE
                     HHKIFVVEVRWGDKLLGLGKGSTKKSAHQEAARMACQKLGI"
     gene            complement(12936..14177)
                     /gene="fabF"
                     /locus_tag="PLB65_03130"
     CDS             complement(12936..14177)
                     /gene="fabF"
                     /locus_tag="PLB65_03130"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HOQ77550.1"
                     /translation="MNKRRVVITGLGAITPVGHNVSQTWQSLINGVSGVGLISRFDID
                     NLPVHIAAEVKNYNPEDHFEFKEIKKLDLYAQFAMIAAREAIADAGLTPNSYDPNRVG
                     VITGAGIGGILTFEEECIKNYTMGPRRVSPFFIPKMIGNIAAAHISIENNFKGINFNV
                     MSACASANHAIGTAYRAIQYGDADIILSGGNEAAVTPLSIAGFSAMRALSTRNDDPEK
                     ASRPFDAERDGFIMSEGAAILVLEELEHAIARGAKIYAELIGYGASEDAFHITAPTEN
                     GEGSARAMLMAINDAGIKPTDIDYINAHGTSTPLNDKGETHSIKTVFGDYAYKVKINS
                     TKSMVGHMLGAAAGIEAIVCVKTIENGIIHPTINLTHPDPECDLDYTPNVAIHHQVDI
                     TLSNSLGFGGHNSAIIIKRYQ"
     gene            complement(14343..14579)
                     /locus_tag="PLB65_03135"
     CDS             complement(14343..14579)
                     /locus_tag="PLB65_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HOQ77551.1"
                     /translation="MMDIEEKVKQIVAKKLGVPESQVVPSANFIDDLRADSLDTVELV
                     MAFEEEFDLTIPEEDQDKLKTVGQAIDYLKEKLG"
     gene            complement(14625..15374)
                     /gene="fabG"
                     /locus_tag="PLB65_03140"
     CDS             complement(14625..15374)
                     /gene="fabG"
                     /locus_tag="PLB65_03140"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HOQ77552.1"
                     /translation="MKLDFTDKVIVITGAARGIGFAIAKTFANANAKVIIIDVASEAV
                     EKAIAELQENGFQAYGYVGNVTDAKSIETIFADVVDKYGTIDCLINNAGITRDNLLIR
                     MKEEDWQLVMDINLKGSFLCTQKAFKYMMRSRNGCIINIASVIGIMGNSGQANYAASK
                     GGLIAFTKSCAKEFGSRNIRVNAVAPGFIETEMTASLSSETRAEYAKAIPLQKMGTPM
                     DIANVCLFLASDAGSYLTGQTIAVDGGLTMH"
     gene            complement(15412..16344)
                     /gene="fabD"
                     /locus_tag="PLB65_03145"
     CDS             complement(15412..16344)
                     /gene="fabD"
                     /locus_tag="PLB65_03145"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HOQ77553.1"
                     /translation="MKTAFVFPGQGAQYIGMASDYLQKEENLKAILNDFDKAHNINLA
                     TVMAEGPEEILKQTSYTQPAILFHSVAALNAFQTKSNLKPDFVAGHSLGEFSALVAAG
                     MISLADALHLVHKRGAFMIKANADVPFGMSAIIGLEADQVIQICRQASVVGLVEAVNF
                     NTPMQTVISGTEEGVLRAGELARMSGAKRVIPLVVGGPFHTPLIVKASEWLMEEMQKV
                     EFKDIEIPVISNVTAAPVTSGKEERELLAKQIVSPVLWSQSVQFMIDQGVTRFIEFGP
                     QKVLSGMIRNINRDAEVFSIDKYEDIDAVVSVTN"
     gene            complement(16364..17359)
                     /locus_tag="PLB65_03150"
     CDS             complement(16364..17359)
                     /locus_tag="PLB65_03150"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOQ77554.1"
                     /translation="MSKTYNAKFSSFGSFTPAKILNNFDLEKIVDTSDEWIRTRTGMF
                     ERHIASENEAASDLAYQAAVRAIESSSVKYKDIDHIIIATISGDHPFPSTACVIQKKL
                     GLKNIPAFDISAGCTGFIYAIDVARQYVENGAAQNILVIGVEILTKITNWKDRNTCVL
                     FGDAAGAAIISRAESSEISLIIDSLIEADGSQGEYLIQKAGGSRMPASHETVDQNLHT
                     VYMEGNRIFKSAIKCMYAACTEILARNDLKVADIDWIIPHQANLRIIEALASKLKVPM
                     SKIIVNIEKYGNTSSATIPLAVDEAIRAQKIRRGDKILLTSFGAGLTWGSVLARY"
     gene            complement(17362..18357)
                     /gene="plsX"
                     /locus_tag="PLB65_03155"
     CDS             complement(17362..18357)
                     /gene="plsX"
                     /locus_tag="PLB65_03155"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOQ77555.1"
                     /translation="MRIAVDAFGTDNAPYPEIEGAVLAIKEDICDEVILVGDEQIINK
                     ELGKFFYPQNRISVVNASQRIEMEDSAAAAIRTKKDSSLVRAIELHKQGLADAVVSAG
                     NTGAVMAASLLIYRCIKNVQRPAIAVVFPTQEGHQILLDMGANVDCSAENLLQFAKLG
                     SMYFEYFFQTKNPRISLLNIGEESAKGNATTKQAYKLLSQDKELNFTGNIEGKDIIRG
                     ITDVVVCDGFVGNIVLKTVEGVGFSIFEILKEEINKDWIAKIGSLLSYPVYSYIKNKL
                     DHTEYGGALLVGLNGISVVAHGRSNAKAIKNAIRFADRIAKSGFVTHAKAYFERF"
     gene            complement(18359..18538)
                     /gene="rpmF"
                     /locus_tag="PLB65_03160"
     CDS             complement(18359..18538)
                     /gene="rpmF"
                     /locus_tag="PLB65_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOQ77556.1"
                     /translation="MAVPKRRTSKMRRDKRRAHRALTIPAFSTCKKCAEPVRPHHICE
                     KCGTYNGKKILAEKE"
     gene            18983..19432
                     /locus_tag="PLB65_03165"
     CDS             18983..19432
                     /locus_tag="PLB65_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77557.1"
                     /translation="MARQRTLVNLVSKSKVHKKEVPHFCFNAMMKGSKKDMLVLYEQL
                     SLGKDKKRFDSLAIMKFGDSNYRLRPKRKAQVPYFAGAYSSLGGTMVSINCGTRDEYR
                     CGKGIIPIHFLNKTNRSLISCTDSGATSKIRYKKQEHIALYFGGSSE"
     gene            19425..19751
                     /gene="rpsJ"
                     /locus_tag="PLB65_03170"
     CDS             19425..19751
                     /gene="rpsJ"
                     /locus_tag="PLB65_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HOQ77558.1"
                     /translation="MSKHDNRIRIKLKAYDHRLLDKSVAEIVKSTRNTGAKVVGPIPL
                     PTDRTIYSVLRSPHSDKKSQDQFQMLVHKRLIDIVNPTQQTTNALKKLSLPAGVHVEI
                     KANTRS"
     gene            19755..20387
                     /gene="rplC"
                     /locus_tag="PLB65_03175"
     CDS             19755..20387
                     /gene="rplC"
                     /locus_tag="PLB65_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HOQ77559.1"
                     /translation="MIGLIGKKIGMTQVFDSDGKVVPVTVIKAGPCKVICKRTEAENG
                     YEALQLGFEEIPERKVSKPMQGHFKKYNCSYYRYVREFRPTHGQNIDSVQIGDELKAD
                     IFGEKDSISVSANSKGRGFTGVMKRHNFGGFISSHGTHETFRGPGSIGQCAQPSRVFK
                     GIKMAGHHGNSKVTVRHLTVVKVDPENNLIMVKGAVPGHRNSLVTIHKEQ"
     gene            20399..21034
                     /gene="rplD"
                     /locus_tag="PLB65_03180"
     CDS             20399..21034
                     /gene="rplD"
                     /locus_tag="PLB65_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HOQ77560.1"
                     /translation="MITAKRYNMQGDLLGEVNLPDNIFNVDVNSPKVLLNEVVTMYLA
                     NQRQGTVQKKNRSMTAGSTRKLFRQKGTGNARMGARRSPVRVHGGKAFAIYPKDWYRK
                     IPRTKKRLALKVALSDRAREGRIMIVEKLSFEKPSAKRALEFMNKIAPEKGYKLVITD
                     GHHIPTVKSFSNLPDVLTDRADSIHAYEVLKSSYIIMTDDALKKVEEVFSQ"
     gene            21031..21318
                     /gene="rplW"
                     /locus_tag="PLB65_03185"
     CDS             21031..21318
                     /gene="rplW"
                     /locus_tag="PLB65_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HOQ77561.1"
                     /translation="MIHPRNIIISPIITEKSSNQVQKQNTYSFKVSGNANKIEIKKAI
                     ERIFDVKVLSVNTICYQGKPKSLGKYNGKRPDWKKAIVTLRQGDKIADFEV"
     gene            21330..22160
                     /gene="rplB"
                     /locus_tag="PLB65_03190"
     CDS             21330..22160
                     /gene="rplB"
                     /locus_tag="PLB65_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HOQ77562.1"
                     /translation="MGIKKYRPTTSTLRFRTGYTFEEITTDKPEKSLLKSKPKTGGRN
                     NSGRITCRHMGGGHRHHYRIIDFKRDKIGIPAKVASIEYDPNRTARIALLHYVDGEKR
                     YILAPEGLEVGAKVMSGPDAEITVGNAIPLERIPLGSVVHNIELKRGKGGQIARSAGT
                     YAQVVAKDGDYVHVKMPSNDVHLIRKECLATIGSVSNPDHSLIKIGKAGRKRWMGIRP
                     SVRGVAMNPVDHPMGGGEGKTSGGGHPVSPWGKPAKGGKTRKTRKYSDKYIVKAVKKR
                     "
     gene            22178..22456
                     /gene="rpsS"
                     /locus_tag="PLB65_03195"
     CDS             22178..22456
                     /gene="rpsS"
                     /locus_tag="PLB65_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HOQ77563.1"
                     /translation="MARSIKKGPFIDAHLLKKVEVLNAEGKKSVIKTWSRRSTITPLF
                     IGHTFSVHNGHKFVPVYITENMVGHKLGEFSPTRTYRGHKEKKKKVTR"
     gene            22466..22804
                     /gene="rplV"
                     /locus_tag="PLB65_03200"
     CDS             22466..22804
                     /gene="rplV"
                     /locus_tag="PLB65_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HOQ77564.1"
                     /translation="MEATATLRYARGSARKARLVLDTIRYKKVTEAQNILRFSNRRAA
                     ATINKLLASAIANAQNKDPKVNLDQLYVTLAIADEGPQMKRYMPRAQGRAFMIRKQTC
                     HISLEIQTIE"
     gene            22818..23474
                     /gene="rpsC"
                     /locus_tag="PLB65_03205"
                     /pseudo
     CDS             22818..23474
                     /gene="rpsC"
                     /locus_tag="PLB65_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529946.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
     gene            23478..23897
                     /gene="rplP"
                     /locus_tag="PLB65_03210"
     CDS             23478..23897
                     /gene="rplP"
                     /locus_tag="PLB65_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HOQ77565.1"
                     /translation="MLAPKKVKHRKMMKGRRKGLSWTGCNVSFGDYGLIALEDSFISS
                     RQIEAARIAITRHMKRVGKVWIRIFPDKPITQKPAETRMGKGKGAPEYWVAVVRPGRV
                     LFEIEGVDAKVAKEALRLAAHKLPIKTRFITREGVEL"
     gene            23894..24088
                     /gene="rpmC"
                     /locus_tag="PLB65_03215"
     CDS             23894..24088
                     /gene="rpmC"
                     /locus_tag="PLB65_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HOQ77566.1"
                     /translation="MKADELRDLSIDELQVKLEELRIELFNLRFQKAKNLLDRPDRIR
                     NVRHSIARIYTIMKEKEKKD"
     gene            24115..24351
                     /gene="rpsQ"
                     /locus_tag="PLB65_03220"
     CDS             24115..24351
                     /gene="rpsQ"
                     /locus_tag="PLB65_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOQ77567.1"
                     /translation="MIKQGIVVSDKNDKTIVVRVERQFIHPLYKKTVRRHKKFMAHDA
                     NNAAHIGDVVQIVESRPLSARKRWALHKIVERSK"
     gene            24367..24735
                     /gene="rplN"
                     /locus_tag="PLB65_03225"
     CDS             24367..24735
                     /gene="rplN"
                     /locus_tag="PLB65_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOQ77568.1"
                     /translation="MIQAQTMLNIADNSGAKKAMCIKVLGGTKRKYASIGDVIVVAIK
                     SATPGGRVKKGSVEKAVIVRTRKEIRRPDGSYIRFADNAAVIIDEKYEPKGTRIFGPV
                     ARELREAHYMKIVSLAPEVL"
     gene            24759..25064
                     /gene="rplX"
                     /locus_tag="PLB65_03230"
     CDS             24759..25064
                     /gene="rplX"
                     /locus_tag="PLB65_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOQ77569.1"
                     /translation="MHFKKGDLVKVISGDDRGKKGHILKVFPKTGKVIVEKVHLIKKH
                     SKPTQQNPQGGIITMEAPINASNVMLFNEKLNAVSKPVYQIREGRRIRVCKKTGDEI"
     gene            25078..25623
                     /gene="rplE"
                     /locus_tag="PLB65_03235"
     CDS             25078..25623
                     /gene="rplE"
                     /locus_tag="PLB65_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOQ77570.1"
                     /translation="MNRLKERYKTQVVEALTKRFGYHNPHQVPKMEKIVVSMGVGQAT
                     QNKALLDNAVKDMELICGRKPVVTKARKSISNFKLRKGMPIGCKVTLRNEVMYEFYDR
                     LISLAIPRIRDFRGIPVDAFDGRGNFSFGLKEQTVFPEIDYDKIDTTRGLNITIVTTA
                     KTDEECRALLQEMGMPFQKSE"
     gene            25633..25818
                     /locus_tag="PLB65_03240"
     CDS             25633..25818
                     /locus_tag="PLB65_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003638073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HOQ77571.1"
                     /translation="MAKKSLIIKQQRTPKFKVRKYNRCKICGRPRAYMRDFGMCRLCF
                     RKYASEGQIPGITRSSW"
     gene            25836..26249
                     /gene="rpsH"
                     /locus_tag="PLB65_03245"
     CDS             25836..26249
                     /gene="rpsH"
                     /locus_tag="PLB65_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOQ77572.1"
                     /translation="MSISDPIADALTKIRNAYRAGHSQVIVNHNKLVEALVNILAEEN
                     FVNSVQVLDRDPENHINYKRILVILRYTNDGEPVLQGIQRISKPGRRVYVKADNLPCV
                     YNHTGCAIISTSEGVMVDRDARLKRVGGEYVCKVW"
     gene            26261..26833
                     /gene="rplF"
                     /locus_tag="PLB65_03250"
     CDS             26261..26833
                     /gene="rplF"
                     /locus_tag="PLB65_03250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOQ77573.1"
                     /translation="MSRIGKAPIKLNSETKVNVTNNVITVKGKLGELKWTLLPGITIE
                     IEDSVLRVNRADDSKDQRALHGLTRALLQNMVTGVTEGYQKTLHIYGTGYSSEVVGPW
                     LRLSLGFSHDILLQIPKELQVSAEAVPRSKGTRSDFQSIITIKGIDKQLVGQFAAEVR
                     NCRPPENYKGKGIRYIDEHVIIKAGKAGTK"
     gene            26851..27222
                     /gene="rplR"
                     /locus_tag="PLB65_03255"
     CDS             26851..27222
                     /gene="rplR"
                     /locus_tag="PLB65_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOQ77574.1"
                     /translation="MITPSCKIKNDLRKRRHFSIRKRVSGTSERPRLVVFRSLKHIYA
                     QVIDDTKGETLISMSTIAKDMGTLTGKKKTEQSYEVGVKLGEKALAAGIKKVAFDRSG
                     YKYHGRIKALAEGARKAGLDF"
     gene            27232..27750
                     /gene="rpsE"
                     /locus_tag="PLB65_03260"
     CDS             27232..27750
                     /gene="rpsE"
                     /locus_tag="PLB65_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOQ77575.1"
                     /translation="MNYEQNHMEEEKLIEKIIETKRVAKVVKGGRNFSFSAIVVVGDK
                     QGNIGVGNGKANEIVDAIRKAKERAVKNMFKVPIVRGTVPHEIIARYGASRVMIKPAA
                     PGTGIIAGGTTRAIFEAAGIENILCKTLGSNTPTNVVKATINGLKSMRTLSDISRLRN
                     KTMAQLTGQEEK"
     gene            27747..27932
                     /gene="rpmD"
                     /locus_tag="PLB65_03265"
     CDS             27747..27932
                     /gene="rpmD"
                     /locus_tag="PLB65_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOQ77576.1"
                     /translation="MKLKVTQIRSTINRIEDHKRVIKALGLGRIGKSRIHNDSPTIRG
                     MINKVGYLLKVEELEEE"
     gene            27935..28390
                     /gene="rplO"
                     /locus_tag="PLB65_03270"
     CDS             27935..28390
                     /gene="rplO"
                     /locus_tag="PLB65_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOQ77577.1"
                     /translation="MLTLSDLQRSAGRKDKKRLGKGQGSGWGHQAGRGHKGKKARAGG
                     NVPPYFEGGQMPMNRRLPKRGFKNIFRENYRTLNLYRLQYIDITDLDIPTMEKMGLIP
                     SKGKKANAPVKVLAGVKDEFTKTVNIKANAFSQKAKEIIEKNGGKAEVV"
     gene            28413..29705
                     /gene="secY"
                     /locus_tag="PLB65_03275"
     CDS             28413..29705
                     /gene="secY"
                     /locus_tag="PLB65_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOQ77578.1"
                     /translation="MFRIPDLKKKIFFTGLFLVLYRLGSFIPLPGVNATALKAFFEGA
                     REGGNTLFGLLDLFVGGNFERASVFALGIMPYITASIVIQLLGSIIPYFEKLRKEGAE
                     GQKKLNQITRYGTVILAAFNAITITMWLTNLSGGVVPRPNFLFHLTGIITLITGTMIV
                     MWIGEQITEHGIGNGISLIIFAGIIARYPEGFVRMFQKISVDSSYTWKALVTIILMVL
                     VTAAVIFVTEAVRKIPVQYAKRIVGRRVYGGQSTYIPLRVNTAGVIPIIFAQSVLMFP
                     ATIIALVGSNSGFWVTLKNLLSPGSVLYTILYVGLIVFFAYFYTAIVLNPTEMAENMV
                     KYGGHIPGKKPGKKTAEYINNVLVRITLPGAIFFAFIALLPEIMSNQLDLPFYFGGTG
                     LLIVVGVALDTLQQIESHLVMRHYDGFMKKGKLRGRSM"
     gene            29707..30468
                     /gene="map"
                     /locus_tag="PLB65_03280"
     CDS             29707..30468
                     /gene="map"
                     /locus_tag="PLB65_03280"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HOQ77579.1"
                     /translation="MIYIKSASEIEKMRKSCHIVGGLLDALEGLIRPGISTLELDKFA
                     EDYILSRGGKPSFKGYQVDGLPPFSGSICTSINEQIVHGIPNANSILKEGDIIGVDVG
                     VNLNGYNADAARTYRVGKISSEAEKLLQVTKESLYRGIAAAIPGNRIGDISYAIGSFV
                     KSKGYFVADNLTGHGIGRNLHEEPMIPNMGKPGRGPRLSSGMTLAIEPMVNIGTNRVF
                     ENGWEFKVWDNSLSAHFEHTILITDGEPEILTVAE"
     gene            30481..30699
                     /gene="infA"
                     /locus_tag="PLB65_03285"
     CDS             30481..30699
                     /gene="infA"
                     /locus_tag="PLB65_03285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOQ77580.1"
                     /translation="MSKSGVIEVEGIVTEALPNTTFRVTLENGHEILAHSSGKMRMNY
                     IRILPGDKVKVELSPYDLTRGRITYRYK"
     gene            30747..30863
                     /gene="rpmJ"
                     /locus_tag="PLB65_03290"
     CDS             30747..30863
                     /gene="rpmJ"
                     /locus_tag="PLB65_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOQ77581.1"
                     /translation="MKVKASVKKICKDCRIIKRNGIIRVICSSNPKHKQRQG"
     gene            30875..31243
                     /gene="rpsM"
                     /locus_tag="PLB65_03295"
     CDS             30875..31243
                     /gene="rpsM"
                     /locus_tag="PLB65_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HOQ77582.1"
                     /translation="MAHIAGIELPKNKRLFIGLTYIYGIGRTNAKEICKKANIDEMKK
                     VADLTMEEEKILRDIVQNEYVVEGALRTQIAMNIKRLMEIGCYRGLRHKRGLPVRGQR
                     THTNARTRKGPRGSSIKKKK"
     gene            31252..31641
                     /gene="rpsK"
                     /locus_tag="PLB65_03300"
     CDS             31252..31641
                     /gene="rpsK"
                     /locus_tag="PLB65_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HOQ77583.1"
                     /translation="MATKTRTKKKRVRLAFDEGLFYVHSSFNNTIVTLTDRAGNVLAW
                     SSGGKVGNKGSRKSTPFAAQVAASEVAKAGLDMGIQKVGVIVKGPGGGRESAIRAVNA
                     AGMKVTMIKDETPIPHNGCRPPKTRRI"
     gene            31653..32270
                     /gene="rpsD"
                     /locus_tag="PLB65_03305"
     CDS             31653..32270
                     /gene="rpsD"
                     /locus_tag="PLB65_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016666076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HOQ77584.1"
                     /translation="MAKYTGPKARLCRKFGENIYGNAKYDKILSRRKFPPGQHGRTLR
                     RKPTDYNLHLREKQKLRNTYCLLEKQFSNYFKKAAKASGVTGDNLLQLLERRLDNVVY
                     RMGFAVTRMQARQFVNHGHFLVNGRKVDIPSYLLKEGDIIEVRTKSRSMKALVEAMDK
                     TEVTSPYSWLSIDKENFRGQFNSIPAVSEIPVTCDLRLVVEFYSK"
     gene            32296..33477
                     /locus_tag="PLB65_03310"
     CDS             32296..33477
                     /locus_tag="PLB65_03310"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HOQ77585.1"
                     /translation="MMYLEPLQMPETVDYDKETYSRTFGKFEIGPLEPGFGTTLGNTL
                     RRILLSSIQGAAVRFVRIEGLHHEFCPIPGTNSDYIDLILRIKQLVIQSSSIEEVPIV
                     LEHKGKGVITASMIQETPSVKIINKDLYLLEVMEDVDFRLEMIVGIGRGYVSADRQNT
                     EGKAEGFIPIDSIYSPVLKVNFSVSHQRVKERMNFDRLIMEIFSNGAIEPSIALFLAA
                     KILKDMAAKISLFETEPQYIRDVELDPDLEEKERILQTSVREIELSVRAANCLAQANI
                     ETIGDLVSKSEAEMLKFRNFGKKSLEEIVQKLKKYELHLGMDVEGIYRQIREARSRGV
                     LPPEEKEIPIPEAKPKAKPKTKTAAEKVNTVEKKTSETATATPPHKPVPSPKKKVKNE
                     T"
     gene            33467..33874
                     /gene="rplQ"
                     /locus_tag="PLB65_03315"
     CDS             33467..33874
                     /gene="rplQ"
                     /locus_tag="PLB65_03315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HOQ77586.1"
                     /translation="MRHRVEGRKFGREMDARRLMMRNLVKSMIEHGQLKTTLPKAKEM
                     RGYVERIITYGKNDTVHSRRLAYSVLGDRTLVKKVFDEIAPAFEGRNGGYTRVLKAGF
                     RKGDNAPMAIIQFVEESTIKPKKNAIKAKDLNK"
     gene            complement(34560..37043)
                     /gene="leuS"
                     /locus_tag="PLB65_03320"
     CDS             complement(34560..37043)
                     /gene="leuS"
                     /locus_tag="PLB65_03320"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HOQ77587.1"
                     /translation="MEYRFNEIEKKWQNVWREKGIFNAPNTSSKPKYYVLSMFPYASG
                     VLHVGHASNYFIGDVISRLKMMEGFNVMQPIGYDAFGMPAENYAIEHNSHPRLTTEEN
                     IAAMRIQFDSMGFGFDWSREISTCRPEYYRWGQYLFKKLYEKGLVYRKKGFQNWCEKC
                     QTVLANEQVEDGHCWRCGNIVEQKDLEQWYFKITEYAEELLDFSQVIDWPERVITMQK
                     NWIGRSEGAKVDFIVEGTNENIPIFTTRPDTIYGVTFMALPPEHPLVQKWLKEEPDNK
                     ELQDFCHKVINEDKVLRSSAETIKEGIFSGRYAINPFNGNKVQIWITNYVLMDYGTGA
                     VMAVPAHDQRDYEFAKKYDIPIKIVIQKPDMSLKLEEMQEAYIESGIMVNSDKFNGMD
                     SEEAISAITNWAVENGWGEKTVSYRLRDWCISRQRYWGNPIPVIHCPHCGIVLVPDED
                     LPVKLPDNVQVGRTTRNPLLSVPEWINVPCPKCGAPAKRETDTMDTFVDSSWYYARYT
                     DPKNDKQPFSRESANYWLPVDQYIGGIEHACMHLLYARFFHKVMRDLGWVNCDEPFAR
                     LLTQGMVTKDGAKMSKSKGNVVDPKYIIDRFGADTLRTFLLFASPPEKDVEWSDDGIM
                     GAFRFLNRVWRLIDSNLEVIKCGLQSTECSDNISEEIKELRYETHYAIYRWREDCLQR
                     LQFNTAIATCMEFLNAIVKIKDPGKLNAAEQQIYAFACITLPKMLYPFAPHIAEELWQ
                     KLGNENMLNDCGLPEYEEQYLTRNIVTYVIQVNGKIRGKLEALADINQDELQAKALEN
                     ENVKRYLDGLNIKKVIVVPGKMISIAAGK"
BASE COUNT        11823 a         6945 c         7385 g        11093 t
ORIGIN      
        1 accactcgtc cttctctcct cctcaacgac gggttgagga cgggaggagg aggagtggtg
       61 ttagaggaag aagcaatagc gaagtagtaa catattcagc gacaacagga taaacggtta
      121 ctgaacaggg atgattcttt ccactacagg tgccaggtta aaagcgattc ttaaaatgcc
      181 atttatgtag cttattttgg ggttctcttt atcgatgctg atatccggga agaagattct
      241 gcggtcgaat ttgccggttt cgatctccat attatagtag cagaggattg aattgcaatt
      301 aggaaagagg cgtgttccgc ttatcctgat gtattctgga gaaatggata tgttgatatc
      361 ttctttttct attccagcca tttctgcgat aacaacccat tgtgtatcgg tttgaaatac
      421 atcacatttg gggtgccaaa catcatctat ggcatcttcc aaagccagag ggctatctgt
      481 tagggcagat acctcgccga tcaattttag catttcccta tgtaatccaa ccaggttagt
      541 gaaaattttt gtgcgcatag taaaactccg tatgtttttt tactcgctgc gttgtctgaa
      601 agtccaaaca atttctttat cgggtgcgac atcgatgctg aaagtaactt tatcgttatt
      661 atctttagtg tatttataat cggcttccac gatgcgggta ttaggggaga gctggtgagt
      721 tactttaata gttttgttgg tatccgaatt attccttaaa gttacttgga tgagactttc
      781 ggtgatatat ttggagattt gtgtttgttc tttcagacgg gtagaagcaa ttaagtcaaa
      841 aggggttccg gtatttatag atacctcttc atttttagag gtgtggttaa tgctgtcctc
      901 gccaataaat tccagattgc catcgctatc ttgtttatag acctttatga ttcctttggg
      961 aagaggcatt ccggcaccgt tttctgcagt atttttgaat ttgatcatac ttttaatctt
     1021 agtggcatag gtgggatatt catattcaga atatgcttta atgtttttgg aaggatacag
     1081 ttctagttgt tttgtctgat tattagcaaa ggagaccttt tggtctaggg tatacatatg
     1141 gaagtcgtga aaggcctttt catcaaaaga aggagctaat tcttcttgtg ccatacctgc
     1201 agtatagctg attaatccct ttctttcttt ataataacca gaataaacct ggttgatatc
     1261 acctgcgata agctttaaat tcacatcgtt aaatgctttg ccagaagtgt tattgatagt
     1321 aacccaggaa ttaaattgta aggtcttttc gtcccaaact gtgttgtatg taacatccca
     1381 gctgaagcca cctgttaaat aagacatttg aacagggtgt ttcccttttt tagaagcaat
     1441 tatattccaa tgtaaagttg gttttgtgta aaaattttct ggtagttctg ccagctgaat
     1501 ccactgggtt tcgctttcgg aaattactaa taagcgatca gttccttttt caataatacc
     1561 gatgctgttg tcgctgtaaa attttagaat tccggttaat ctggattggt ccttagtaat
     1621 aaccagaact tctctatcca gatatttagc cataatctgc cattttccgg caagatcgta
     1681 ttcgtaattc tgctccgcaa tgcgaattcc atttccggta acaattacgg atgcaggatc
     1741 tattctacta gttatatcgc tataattgat atcctggcga ccctctacca gctcaagttc
     1801 aaaacggtta cgaactaaag aaagatcatt attgtatatt gttaaccaat cttcggctaa
     1861 cagaggtatg gtaagcatta gaaggatagt ggaaatgaga atgtttttca ttatatctcc
     1921 ttataataga cgaattctga ataaatctca tcatatatac aatttaattc tattatctat
     1981 tatgtcaagt gaaaacctga tgaaaatatg ggaagaacag catcctgtgt ttcttttaca
     2041 acaaagttat gtagttactt gttacttctg ggtaacttat ttagtatttt gttgtagttt
     2101 gtgaggatat caatcctttg gtaggttagg aatctttacg aacataatgc tttgcggcgg
     2161 aacggcgtcc gccggctaca tttttttgcg gaggaacagc gtccnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnggctac atttttttgc ggcggaacgg cgtccgccgg
     2341 ctacctaaaa ctatccccgg gttttacctt tccacattct cttcgcataa gaaactcgaa
     2401 ataaagattg acaattttat ccactaatat ttaaagaaat agaagtatat ttaaggttta
     2461 gcaagtggga ggattttatg aaaaaagtgc ttggactgct gatcattgtg ctgtttttaa
     2521 tgtcctgttc agatgaacca accaaagtta ctgttcgtga tgataatcag gataaagtgg
     2581 cgcatttggg tcctgatcca aatttattag gcaacacgga aatatttttt atcccgggaa
     2641 caattcaggg ttcagctatc tggataataa acggtcctgg tgcaaatgta ggtattgata
     2701 tccgagaccg ggaaaattcg agttttgttt attatgctga tagttatatt ggttctggat
     2761 ccaattctgc gcaaacggga actcaaattc cctggaacaa atggatgagg gtgcggttgg
     2821 cggtttataa atccggactt tctggcatta ttgtaaactt cattcaagcg cttggtttgg
     2881 atttttttga ttctttggaa agttatatga tagagcagat ttatgaaaac gatgtatatt
     2941 taagcggaaa tggaactcag ataaaaacgc cggtaaagtg ctataatgat attagcaagg
     3001 taactggagg aagtattcaa gttcattaag gcattaaatg taactgttga tatactttgc
     3061 ggaggaacgg cgtcctccga ctacacaaaa aaaacagagg aagagcgttc ccttaactac
     3121 aaattttatg attatacttg acggataaca ggttttgata aattatgaaa ataaatgtat
     3181 agaggtgtta gaattaaatc tgggagacag ctatgacata ctactatgaa tccccttctg
     3241 ctacaacaac caaacatata gatgaagaac cattagttcc ttctatgaaa gttagcgaat
     3301 ggttaattac attattgctt acttgcattc ccgttgttaa tatcattatg ctgataattt
     3361 ggtcgttaga tacaaaagga aatcccaacc gcagaaattt tgccatagcg gtcttaatct
     3421 ttatggctgc tatgttagtt ctttccggat tttatattgg gcaaataaca ggttggtttt
     3481 ttaaatttct aaatctgatg taaaaaaaag gagaaagtat gaattatctg gatctggcaa
     3541 tgaatgcggc agaaacgctt ggtgctgaat atgccgatat ccgcattcag aaaacaattg
     3601 atcaggtaat ctacctgcaa aacttatccc taaaacatac cagtaacgat gttcaacagg
     3661 gatatggcat ccgggttttt aaaaatggtg cttggggttt tgcgcacaat aatgttttta
     3721 gtgatgaagc agttttggct acagtaaaaa aggcgtatga aactgctatc ctttctgcgg
     3781 aagttaataa agacaagaaa ttgagtttag ccccggaacg cagttacctt gcaacttata
     3841 aaactcccat taaaattgat ccttttaatg ttcctttaac agagaagata gatctgctgc
     3901 tggaagtgaa tagagtaatg ctggcttacg aaggaatcaa gcaagctcgg tctctattaa
     3961 ttatgcataa agatgaaaaa ctttttgcca acactttagg aacgcgttta gatctttcca
     4021 cccattttat taatccagtg attacagctg tagctgttac agaagatgac agtcaaagcc
     4081 gcacttttga tgaaggtgga cgagcaatag gttgggaatg gattttggaa ctggatttaa
     4141 ttaatatagc caaacaaatt gccgaagaag cactaatcaa agttaaagct gatactcttg
     4201 gtgaagaaca acgccgagca ttgattcttg atcccaatca tctgggtttg acgatgcatg
     4261 aaagtgttgg ccacgctacg gagttggata gagttttggg ttgggaagct gattatgccg
     4321 gtgtttcttt tgccactcct gaaaaattga acaactatca atatggtagc gaattgatta
     4381 actttgtagg agataacact ttggaaggtg ggcttgccac tctcggtttt gacgatgatg
     4441 gtgttcccgg tcagaaatgg tatattataa aagatggtat tttgaaagaa tatggcagca
     4501 cgcgagacac tgcattagaa atcggtttaa attcttcccg tggatgcaac cgtgctactt
     4561 actattttaa tcagccaata aaccgaatac ccaatttgta tttactgcct ggaaccaaac
     4621 ccttaacccc aggtgaactt attgccgata cggaagatgg agtatatatt caagggagag
     4681 ggagctattc tatagatcat catagaatta acttccagtt tggcggcgat ttcttttggg
     4741 agatcaagaa cggaaaattg ctgcgtccct taaaaaaagt aatctacaaa tcctataatc
     4801 cagaattttg gaacagctgc gacgcaattt gtgatgaaag attttggcgt cctttcggag
     4861 tagtaaattg tgggaaaggt caaccatctc aaacagcaag aatgacgcat ggttctgcgc
     4921 ctgcaagatt tcgtaatatc cgggtaggag gttcacaatg aatagcgaaa aaatagttaa
     4981 atatatccaa acaaactgca aagcggatga ttggtcccta aatatctctg aggatgatag
     5041 ccatgaaacg cgttttgccc aaaattctat tactcaacac atttctggcg ctacgaagga
     5101 aatctcttta agcgtttctt ttgatggtag aaaaggaagt tgttcggtga atcaggataa
     5161 tgaagaaagt ttggcatatt tgttgaaaac agctgaagat attgctaaat tggcaccggt
     5221 agacccagaa tttgtccctt cggaagggaa aaaggatatc ccggaagttg ataattgcga
     5281 tcctgaaact aaggctcttc agccaaaaca attggtggat attgtgcaaa ccagcatcaa
     5341 tgaagctaaa aaatttggag caaccgtttc tgggttaaca gaaaaacata taagatccga
     5401 tggcctcttc acgaaaaatg gttttgccgg tgaatttgaa tatagcgatt ttggtcattc
     5461 tatgacccta aaaaaggaag aggtggaaac gaaggtctct tatgaagcca agaaattcgc
     5521 tgattttaat ttgaaagacc tttgtgataa attgcaacat caggcaacta cccttgccgt
     5581 taaacaaaac tttgagccac aaaagattgc cgttattttg cgtcctgttg cgttacaaga
     5641 attactgtgg tttatgggtt ggatgatgga ccgccgccag tctgatgaag gttttacacc
     5701 ttttacggat aaaatcggta aacccttctt cggggataaa ttcagctggt attcaacaat
     5761 gcaacagcca caacttttag ctttgccata ttcttccgat gggattatct ctgaagaaat
     5821 tccctggata gaaaaaggaa tattaaaaaa tcttcctgtt agccgctatt gggcaaaaaa
     5881 aataaacagt aaacccagca gcgcatataa tatgtatatt ccgggtgagg gatacactga
     5941 agaagaaatg atgaaaatgg tcccgcgcgg acttattgta aatacttttt ggtatattcg
     6001 cagcgtggat acaaaagcag gtgaatttac cggaacaacg cgtgatggag tttggtattt
     6061 tgaagacgga aaaattaagt atgcagtaaa caatttacgc tttaatgaaa tccctcacga
     6121 tgctacgcgt agaattattt ccacaggcaa aagtgagctt gcttcgccaa gttcgctgct
     6181 cccggctatg ctgatagata actttaattt tgtggataga acaacttttt aaagaaatga
     6241 attgacaaat ttttgaaaac taaaaatagg tgttactaaa tgcgattcaa cattcaccct
     6301 aagccagttc cttttgtttc gggagtgcct ttggaccaga atgttcgatc ccgttaataa
     6361 aaggagatac caatgccaga cagaacactt atttgccagg actgctccaa agaatttgtc
     6421 ttcaccgaag gtgaacaaga attctacaag gaaaaaggcc ttcaaaatga gcccaaacgc
     6481 tgccctgatt gccgtaaagc aaaaaaggct cagtttaatc gtaacagacg cagacaatct
     6541 tactaagaaa gtttattaat ctgttaacgc cactgctatt ttgcggtggc gtttttgttt
     6601 gccttttact gataaaaata aatttcacgc agattacgcg gattacgcag atttttgaga
     6661 aaagagtatt cagggagata ggagaaatca gagtgaatag aggttagggc agtaagtatt
     6721 gttggatagg tctgcattta tcagctttgc agttataaac agcacacaaa agaaagattc
     6781 aggggctcca gaaatcccca gctgcccata ttcaatttat tgcttcagac tcttgctatc
     6841 tttttcccct ttccaagtta tagcaatccc catcttcttc tgaaaaacaa ttccaagcaa
     6901 aaagtaaaaa tgaagagagc gataacatac cattttttat agaggggtat ttctttacgg
     6961 gtaatttgtt caatcggttt agcgggggta ggattatagg aatcaatatt tgatgcgtac
     7021 agtacttttc ctccagtttc atttgccagc caagagagta agggcaggtt atatccataa
     7081 tctcttgttt caattgaagt ttccgaaagt tcgaatttac ctttcgtgct atttccgctt
     7141 tctttatctg aaatttcaaa gctgtaagtt cccggttcag agagttgcag agaaatagca
     7201 tattcctcac cttcgcgttt taagaaatcg gtatatactt ctttgttgtt tttattcata
     7261 atccgaatgc ggggatttac atttagacgg ctctgtctaa tttcattttc attgcgcaag
     7321 cgaatccgta tttcttctcc ctgaaaatag ctattgttat aaatggcagt atagtttccg
     7381 gtacttttat tgctaagcca agttaagcaa ctgctgatta attttgtgta tccttcttca
     7441 ctgctttgca tttgccaacg ccaaaggttt aaagtagcaa aagctatcga gcgggctgtt
     7501 tttgtatgtt gaacaacgat ggcaggtgaa ttttgggggt tattgatagt tgccaaaata
     7561 tcagttcccg ggctggctgt tacataataa taatccaagg gaggaatatc tttggtattt
     7621 acagttaagc tattcagcat cggataatta gcagctgctg cagtaggtac taaaaaaccc
     7681 tgataaatcg atgtaatatt agatttttga ataggtaaaa tagctgacag ttcgttaacg
     7741 gggagtcctt gatacataat acccacgcct cgttggaagg cattttggat gaaattagct
     7801 gtagaattat ccaagtttag cataccatta ttgataatga ttatagcagc taagttatgg
     7861 gaatccaatt cattaactaa aacttctccg ctaaagagtc gtccatttct atttaaataa
     7921 ctttcagctt cccagcggga atttgtagct atggcatcga gggtgaattt attatcccaa
     7981 gcaggtttat ccgaaataag gataattctc tgtttttccg atagcacttc aattgcaccg
     8041 gggaaaatat tattgttaat ttggctttcg ttaggtaggg gcacaacttc taccttccaa
     8101 gggaaaaaac caatattatt gaagcgctgg gtgaaatcta ctgtgctgat tatccctttt
     8161 tccagtttca tattttgtcg tgagataatt ttattagcca gcaacagacg cacttctgca
     8221 ttgccgctat aattttgtga agaaatttca gctctaatgg tattgggctc atttttataa
     8281 gcatagcgat tgtagcgaac atttacaatt gccaaatcag gagtgggatt tacagtgcta
     8341 tctgccaaaa cataaaaagg gcagcccaat tgttgaacta tagataaaga ttcatctctt
     8401 aaccagccat ctgaggcaag aacaactccc tccagatttc ggaaagcttt ttggttgctc
     8461 aattctttta gggcaggagc cagaagggta gcatttttat ctccgttcaa accgttggca
     8521 aaagttttat gaataacttc atagtcgtta tctgcgaatt ttttctccat tttctgccaa
     8581 agaggtttta agtgttgagc cttggaacca gaattatgtt tcagattcat actttgggaa
     8641 acatcttcca gcaccaaaat ttgagctgtc tgttttttat gctgagtata atataaaatt
     8701 gggctaagta acagaatcaa caacagagat atggaaaaaa acctgaggac aaaaagagtt
     8761 atcctccgtt ttttttcaat ttccggattg gtttgatagt ttaaaaaacc agatatcagc
     8821 agcgagaaca gaacgatcag agtgtatttc agcattaaat attgaccttg atatagcttc
     8881 ctatagataa tcctttagat ttataacttg gaaaaagctg tttaatactc tgcaccccaa
     8941 aaccgatttc accggtgctt gtatgcaaac caattgaata actaactcgc cagggatata
     9001 tgctattacc aaaacacact cccataccga tgggtaaaaa ctttaaaggc aagacctgag
     9061 cagccataga aatacggcct atattgctat tgtttattga gcttttaaaa ccctgcaaat
     9121 agtctactga taaattagct cttttcattg aataaagagc tcccaaacga agttctggag
     9181 gtagctctgt agaatatgta tcaatatcta cggtttcaga ggtataatca taaaattcct
     9241 catctatatt ggaagcataa acatcatctg ctgtaatact aatatttctt gcttcggttg
     9301 taaaaggcca ggttattttt ccaaataggt tatccaaggt aaggcctact tctaattggg
     9361 gagtaacatt actggccaga ccaagcataa ctttttgacc atagccacca atacctgtat
     9421 gtaaagaaat atcctgttgt aaagataagc cttccataga agaattaaaa taaccattaa
     9481 attcttgtgt attagcatca ccaacaccta ctaaagcgct cacggaaaag ccagcgcgaa
     9541 tttttggtat cggttcagga ataaaaggta tgataaaatt acccattcca aaagtgaagt
     9601 cagcatagct caaaccgcta atatcggtag tgcttttatc aaaaacatat aatgaatcgg
     9661 tattaccata aagcaatagg tccacaaatc tttccgatac tgccagcctt ccagttaaat
     9721 gaagagaact agaaaaggaa atattattaa aagcaaaacc aataatactg atattacctt
     9781 ctacagctga acgcagagaa ccatcaattt tgttcaatat tttaatttta tcttcatcat
     9841 ctaagaaatc ttggcttact acataattat aggtatccag atccagagag ttattattta
     9901 cggatattcc caggttcata atcggtagcc aaaaatcaat accctttact tgttttaaat
     9961 tagcgggatt ccaataggca gcttctacac ctttggcacg cagcatatag ctatcagcaa
    10021 acaggacaga tttacctgta ttaaatgcat ttaaaacact aacagatatc agaaagataa
    10081 gcactaataa tatacacctt ttcatttcaa tcctcctcta ttagtatttc ggcagagatc
    10141 attcctttaa gatgaatata atctccactg ctggcgtgaa tagtaacagg agaacctgtt
    10201 tcttcaaaac tgaaagacca ccgcatataa acttgttctg aagcaaaaag atcaagttca
    10261 tctttagtca attccagctc cacatcctga aaaccaggat tctgattgaa gctatgaatc
    10321 tccacttgct tcagaaaaga ataagtacct ggattatgga tatctatatt gggagtattg
    10381 cttaagaata acttagctga agccccaatt ggaagcatat tttgaatttg catattcaaa
    10441 gaagcactta aagcgttttt ttggatgcgc tcgcggttat cttctggaat actgaattca
    10501 tgctcttcat taataacaat ttcgtgttcg tgtaaagtaa aaataagagg agcattaatc
    10561 aaataatcca aatgcatagt atcggtaggt tttacacttc cgatattatt atcactttca
    10621 atttcaaaac accctccaat aacctgaata gtagttggca tcagctgtaa gagttctgta
    10681 atattatcat atagattaaa attcataacc acagggccat cttgcaatgc tggttgtatc
    10741 tgaaaattgt ttccattttc atccaaaata tcaattatgc gagaaacacc tgcagcatta
    10801 gtagctttta attgtcctga aaattctgct ccaaaaccta tataattctg caaagtaagc
    10861 tgaagtgaag cttcttccag agttaaagtt tgatctaagc catgaggata atcaatatta
    10921 attgaagaat ccgaatcatt caaattaacg ggataattgt gtaaaccacc tctaaattct
    10981 cccaaactaa tcattgaatc tgatctaaaa tctaaagtag ctacttgtgt cccagcagga
    11041 agagtagaat tcacttgtat agagatatgt aactgggtta ttatttcgtt agtattcggc
    11101 gttcccaaat gcattccatc caaatttgtc gtaacccaat tatcgaagtt atcgctaata
    11161 attaccaagt tctgattgct attgggcaga taaatatcgt gaaaaaccaa agaaatttga
    11221 tcaacatttg gatcaacatt tctcaggcga ctttcaaaaa caccttctgc aagctcagca
    11281 tatgcaatct caactgtacc agtgggatct gtaaaaggaa tataaaaatc ataactcgag
    11341 ccaattagag gaattccagt taaattaata ttaggattaa aaggtaaatt gccaaattcg
    11401 tttgttgttg cctcaccatt agctgtaact gttaagatat tatcttcatt caaaacgatg
    11461 tttacagaat ctaccaaatc agacacataa aaaaaatcgt tgatgagggg aatatttaat
    11521 ttaacactcc agcttggcat tgtgggtttt ttcacctcgc aagcaccaag aaatactaaa
    11581 gcggtaatca gaataaaata atactttttc ataagaaaat ccttttgctg cttaagttct
    11641 attaagagct cttctcatag agatcgagga aataacttct ccccaaacca atatagacat
    11701 tataacattc ttgaaaaaca aaaatcacac gattaattcc taccgatgat aatgataatg
    11761 tagttaaaga caaaataaac aattgctgcc ttttggcaag ttatttctaa gaagattgaa
    11821 aactaaagca gctccaataa taataatgtg tttaacaaaa ctctaagaag gcaatagaat
    11881 aatattactt ccgaaatgag aaaggcgtgc agcatacaaa aagagtatat aataggtttg
    11941 cttatattta gttggaactg ccaatttgtg caatccaatt aaccgtaaac tgcattattt
    12001 tctagtacct ggcttacggg ttcttctctt gcagttttat tacgattaac tacactaaaa
    12061 aacaatagga ttagcgttca actaagggta caatatgggt tcgccatctc tcgctgtata
    12121 tgttttcctt tattatatac caagtttctg gcaggccatt cttgcggctt cctgatgtgc
    12181 actttttttg gtgcttccct ttcccaggcc taaaagttta tcgccccaac gaacttccac
    12241 aacaaaaatc ttatggtgct cgggtccttc ttcggcgata gtaacataat ttggtggtcc
    12301 ttgatttctg ctttgcatat attcttgcaa aatacttttg taatttacca gctcttcgcg
    12361 gttaacggtt tcttgataat tatccagaat aaaacgacgg ataaatttgc tggctgcaga
    12421 tattccacta tccagataaa tagcacaaat tagagcttcc attgtatccg acaggataga
    12481 aggtttggaa gctccccctg cttgttgttc ttctggactt aaaagtacat atttaccgat
    12541 gtctatcgca tttgctttga aagttaagta agtttcggaa acaattttgg attttagttt
    12601 ggataatttg ccttcctgct cttcagggtg ttcggtaaat agttctttac ttacaatgaa
    12661 tccaagaatg gaatcgccca gaaactccat ccgttcaaaa ggagaaggag ttttatgttc
    12721 actataatga cgatggagat aggatttatg tgttaaagca gcatgcaata aagtggggtc
    12781 atgaaaagta taatcaattt tcttttgcaa ctgcgtaagt gatttttccc atttgggata
    12841 cttttcacct atctttttag tgttgaaata ctcaacaatc cgggagatga ttgattttgt
    12901 ccttttctcc atcgatgtat atatcccggc ttgatttatt gatagcgttt aatgattata
    12961 gcagaattat ggcctccaaa accaagggaa ttggaaaggg ttatatctac ttggtgatga
    13021 atagctacat taggagtgta atccaaatca cattctggat caggatgagt taaatttatt
    13081 gtaggatgaa tgatcccatt ttcaattgtc ttaacacaaa cgatagcttc aatccctgca
    13141 gcagctccta acatatgacc aaccatactt ttagtggaat ttatctttac tttgtaagca
    13201 taatctccaa aaacagtttt gatggaatga gtttcgcctt tatcatttaa aggtgtagaa
    13261 gtgccatgtg catttatata atcaatatct gtaggtttta taccagcatc atttattgcc
    13321 attaacatag cccgtgcact tccttctcca ttttctgtag gagcagttat gtgaaaagca
    13381 tcttcagaag caccgtaacc tattaattca gcataaattt tagcacctct ggctatagca
    13441 tgttccagtt cttccaaaac aagaattgct gctccctcac tcattataaa accatcccgt
    13501 tctgcatcaa aaggacgcga tgctttttca ggatcatcat tacgagtaga aagagctctc
    13561 attgcagaaa aaccagctat cgataaagga gttacagctg cttcatttcc accgctaaga
    13621 attatatctg catccccata ttgaatagct ctataagctg ttcctatagc atgattggca
    13681 ctggcacaag cactcatcac attgaaattg atacctttga agttattctc tatactaata
    13741 tgggcagcag caatgttacc aatcattttg ggtataaaaa aaggactcac tcgacgggga
    13801 cccattgtat aattcttgat gcattcttct tcaaaagtaa gaataccacc aattcctgca
    13861 ccggttatta ctcctacccg attggggtca taagaatttg gagtaagacc tgcatcagca
    13921 attgcttcgc gggctgcaat catagcaaat tgagcatata aatccagctt ttttatctct
    13981 ttaaattcaa aatgatcttc aggattgtaa ttcttcactt cggcagcaat atgaaccggc
    14041 aaattatcta tatcaaatct tgatattaat cctacgccag aaactccgtt aataagggat
    14101 tgccaggttt gggaaacatt gtgtcctaca ggagtaatgg cacctaaacc agttataaca
    14161 actcttcttt tattcataaa aacctcatta agtttaacaa attatttgtc tttaatacca
    14221 cttttctctt cccaaccttg tatttaaggc aaaaaaatca agagaaagca tcctctgtgc
    14281 gtggatacaa tcctgatatc tattatgtta tcaggattga tccacattag ggggatgggt
    14341 atttatccca gtttttcttt cagatagtca atagcttgac cgactgtttt cagtttatcc
    14401 tgatcttctt cagggatagt gagatcaaat tcttcttcga aagccataac gagctcaact
    14461 gtatcgaggg aatcagctcg taagtcatcg atgaaatttg cggaaggaac aacttgggat
    14521 tccgggactc ccagtttttt cgctactatc tgttttactt tttcttcaat atccattatt
    14581 cctcctatgt tattgaagtg attatttatc tattggtttc ccatttaatg cattgttaaa
    14641 ccgccatcta cggctatagt ttgtcccgtt aaataacttc cagcgtcgga ggctagaaag
    14701 agacatacat tagctatatc cataggggtt cccatttttt gcaagggtat ggcttttgca
    14761 tattctgctc tggtttcaga acttaaagat gctgtcatct cagtttctat aaaacctgga
    14821 gcaactgcat taacacgaat attacggcta ccaaattctt ttgcgcaact tttggtgaaa
    14881 gcaataagac cacctttgga agcggcataa ttggcctgtc cactatttcc cataattccg
    14941 ataacactgg cgatattaat aatacagcca ttacgggaac gcatcatata tttaaaagcc
    15001 ttttgagtgc aaagaaaact accttttagg ttaatatcca taaccaattg ccagtcctct
    15061 tctttcattc tgatcaataa attatcgcga gtgattccag cattattgat caggcaatct
    15121 atagttccat atttatcaac tacatcagca aaaatagttt caatactttt ggcgtctgta
    15181 acatttccta catagccata ggcctggaat ccattttctt gtaactctgc tattgctttc
    15241 tctactgctt ctgaagcaac atctataatt ataacttttg catttgcatt agcaaaagtt
    15301 ttagctatgg caaaaccgat cccgcgagcg gcaccagtaa ttacgataac cttatctgta
    15361 aaatcaagtt tcattatctc ctcatctgat tggtttaatt atataaccag cttaatttgt
    15421 aactgacaca actgcatcta tatcttcata tttgtcaata gaaaaaactt ctgcatctct
    15481 attaatgttt ctaatcattc cgcttaaaac cttttgtgga ccgaattcga tgaaacgggt
    15541 aactccttgg tcaatcataa attgaacact ttgcgaccaa agaactgggg aaacaatttg
    15601 ttttgcaaga agttctcttt cttctttacc actggtaaca ggggcagcag tcacattaga
    15661 gataacagga atttctatat ccttaaactc taccttttgc atttcttcca taagccattc
    15721 actggcttta acgattaggg gagtatgaaa aggacctccc actaccaaag gaataactct
    15781 tttggctcca ctcattctgg caagttcacc tgctcgcaaa acaccttctt ccgttcccga
    15841 aataacagtt tgcattggag tgttaaaatt tactgcttct actaatccta caactgatgc
    15901 ctgacgacag atttgaataa cttgatctgc ctccaaaccg attatggcac tcatcccaaa
    15961 agggacatca gcatttgctt tgatcataaa tgcccctctt ttatgaacca agtgtaaagc
    16021 atctgctaaa gaaatcattc cagcagctac caaagcactg aattcaccta aagaatggcc
    16081 tgccacaaaa tctggtttca aattactctt tgtttggaat gcatttaaag ctgcaacact
    16141 atgaaacaaa atagcaggtt gagtataact tgtttgtttt aatatttctt caggaccttc
    16201 tgccattact gttgccagat taatgttatg tgccttatca aaatcattta aaatggcttt
    16261 taggttctct tctttctgta aataatcact tgccatccca atatactgag caccttgacc
    16321 tggaaaaaca aatgcagtct tcataattta acctcttata aacctaatat cttgccagaa
    16381 cactacccca agttaaaccc gctccgaaag aagttagcaa tattttatcg ccgcgtctta
    16441 ttttctgtgc gcgaattgct tcatcaacag ccaaaggaat cgttgctgat gaagtgttcc
    16501 catatttttc tatattcaca attatcttac tcatcgggac ctttagctta gaagccaagg
    16561 cctcaataat tcttaaattt gcctgatggg gaataatcca atcaatatca gccaccttaa
    16621 gatcgttgcg cgccaaaatt tcagtgcaag cggcatacat acattttata gcacttttaa
    16681 atatccgatt tccttccata taaacagtat ggagattttg gtctactgtt tcatggctgg
    16741 caggcattct tgaaccacca gctttttgaa ttaaatattc accctggcta ccatctgctt
    16801 caattaaact atcgattatc aaagatattt cactggattc ggctctactg ataatagcag
    16861 caccagccgc atcaccaaat agaacacaag tattacgatc tttccaatta gtaatctttg
    16921 ttagaatctc tactccaatc accagaatat tttgcgccgc accattttct acatactgac
    16981 gcgctacatc tattgcatat ataaaacctg tgcaaccagc agaaatatca aaggctggaa
    17041 tattcttcaa acccaatttt ttctgaatga cacaagcagt agaaggaaaa ggatgatcac
    17101 cagaaatggt agcaataata atatgatcaa tatccttata tttaaccgat gaactttcta
    17161 tggctctaac cgcagcttga taagccaaat cactggctgc ttcattttca gaagctatat
    17221 gacgttcaaa cattcctgtg cgggttctga tccattcatc actggtgtcc acgatttttt
    17281 ccaaatcaaa attattaagg atcttagcgg gggtaaaact accgaaggaa gagaatttag
    17341 cattgtaagt tttagacata attaaaacct ctcaaaatag gccttggcat gtgtaacaaa
    17401 gccagattta gcaatccgat cggcaaaacg gatagcattt tttattgctt ttgcattact
    17461 gcgtccatga gcaactactg atataccatt taaaccaaca agtaatgctc caccatattc
    17521 agtatggtct agtttatttt ttatgtagga atagacagga taggatagca aagatccaat
    17581 tttggcgatc caatccttat taatctcttc ctttagtatt tcaaaaatgg aaaaacctac
    17641 accttccacc gtctttagaa caatattacc tacaaatcca tcacatacaa ctacatctgt
    17701 gattccgcgg ataatatcct taccttcaat attaccagta aaattaagct ccttatcctg
    17761 actgagcaat ttatatgcct gcttggttgt agcattgccc ttcgcacttt cttctcctat
    17821 attcaaaagc gaaatgcggg gattttttgt ttgaaaaaaa tactcaaaat acatactacc
    17881 cagcttggca aactgtaata aattttctgc tgaacaatct acattggcac ccatatccaa
    17941 taatatctga tgcccttcct gtgtaggaaa aacaactgct atggcaggac gttgaacatt
    18001 ttttatacac ctataaatta aaagagatgc agccattact gcacctgtat ttcctgcact
    18061 gacaacagca tcagcaagac cttgtttatg aagttcaatc gctcttacta aagatgaatc
    18121 cttttttgtc cgaattgcag ctgctgcact atcttccatt tctattctct gactggcatt
    18181 cacaacgctt atcctgttct gaggataaaa gaatttaccc aattctttat tgattatttg
    18241 ttcatctcca accaggataa cttcatcgca aatgtcctct ttgatagcca aaacagcacc
    18301 ttctatttct ggataggggg cattatctgt tccaaaagca tcaacagcga tgcgcacttt
    18361 actccttttc tgccaatatc ttcttgccgt tataggtgcc acatttttcg caaatatgat
    18421 gcggacgaac aggttcagcg cattttttgc aagtactaaa agccggaata gttaaagccc
    18481 tatgtgctct gcgtttatct ctacgcattt tggaagttct tcttttggga acggccatta
    18541 ttattctcct taagctaatt tcttataagc attacacaag cgataacata agatcagaag
    18601 ttaacgattt cccctataag ttaattatgc gcttgagctt tatataaatt tagaggataa
    18661 actttgagag gactgatttt gtcaaccaaa atatatgagc agcggtttat tcctttgtgc
    18721 aaaaagcaga tagagagcta attaagataa ctaaaacgat atcaaaaaga taaactggaa
    18781 gggctattat tatgatcgag cattttcagt tctgcaaaaa aataatatac aataacttac
    18841 agctctccag cacttcttcc ctaaatagct atcggggaat tgtggtgcaa ctgattagca
    18901 aaatcccaga gttatccctt ggtagctgca ccatatataa ttaaggaagg agtaaaaaaa
    18961 atatagtggt aagggagaat aaatggctag gcagcgcacc ttggttaatt tggtatctaa
    19021 gtctaaagtt cataaaaaag aagttccaca tttctgcttt aacgcaatga tgaaaggaag
    19081 caaaaaggat atgctagtgc tatatgaaca gttatctttg ggaaaagaca aaaaaagatt
    19141 tgacagttta gccattatga aatttggtga ttcaaattat agattacgtc ctaagcgtaa
    19201 agcacaagtc ccatatttcg caggggctta tagttcttta ggaggaacaa tggtatccat
    19261 taattgtggt actcgcgatg agtatcgctg tggaaaaggt attatcccta tccacttttt
    19321 aaacaaaact aaccgcagtt tgatttcctg cacagatagc ggagctacat ctaaaattcg
    19381 ctataaaaaa caggagcata ttgctttata ctttggaggt tccagtgagt aaacacgata
    19441 atcgtattcg tataaagttg aaagcatacg accatcgtct attggataag tccgttgctg
    19501 aaatcgtgaa atctactcga aatacgggag ccaaagttgt cggtccaatt ccgttaccta
    19561 ctgatcggac aatttattcg gttttacgaa gtcctcattc tgacaagaaa agtcaggatc
    19621 agtttcagat gctggttcac aagcgtttaa ttgatattgt gaatcctaca cagcaaacca
    19681 ctaatgctct caagaaactt agtttgcctg ctggcgtgca tgtagaaatc aaagctaaca
    19741 cgaggagcta agttatgata ggattaattg ggaaaaaaat aggtatgaca caggtctttg
    19801 acagcgatgg taaagtagtt ccagttacag ttattaaggc cggtccctgt aaggttattt
    19861 gcaaaaggac agaagctgaa aatggttatg aagcgctgca gcttgggttt gaagaaattc
    19921 ctgagcgcaa agtttctaag ccgatgcaag gtcattttaa aaaatacaac tgttcttatt
    19981 accgttatgt gcgtgaattt cgtccaactc atggtcagaa tatagatagc gtacaaattg
    20041 gcgatgaatt gaaggcagat atttttggtg aaaaggactc tatttccgtt tctgcaaatt
    20101 ctaagggacg tggatttacc ggagtgatga agcgccataa ttttggagga ttcatttctt
    20161 cgcatggaac gcatgaaaca tttcgtggtc ctggttcaat aggtcaatgc gctcaacctt
    20221 cccgtgtttt taaagggatt aaaatggccg ggcatcatgg aaattcaaaa gtgactgttc
    20281 gccatttaac tgttgtaaaa gtagatcctg agaataatct aattatggtt aagggtgccg
    20341 ttcctggtca tcgtaattca ttagtaacca ttcataagga acaataggcg gtaatgtgat
    20401 gataacagct aaaagatata atatgcaagg tgacttactt ggcgaggtta atcttccaga
    20461 taacatattt aatgtagatg taaattcgcc caaggttcta ctgaatgaag tagtaacaat
    20521 gtaccttgct aatcaacgtc aaggtacagt acaaaagaaa aacagaagta tgactgcagg
    20581 tagcacgcgt aaacttttta gacaaaaagg aacgggtaat gcgcgaatgg gtgctcgtag
    20641 gagtcctgta agggttcatg gtggaaaagc ttttgctata taccccaagg attggtatcg
    20701 caaaattccg cgcaccaaaa agcgtcttgc tttaaaagtg gcattaagtg accgtgctcg
    20761 cgaaggcaga ataatgattg tagaaaaatt aagttttgaa aagcccagtg ctaagcgtgc
    20821 tttggagttt atgaataaaa tagctcctga aaaaggatat aaacttgtga taacggacgg
    20881 tcatcatatt cctacggtaa agagctttag taatctaccg gatgttttaa cagatcgcgc
    20941 tgatagcatt cacgcttacg aagtgctcaa aagtagctat ataattatga ctgacgacgc
    21001 cctgaaaaaa gtggaggagg tattcagcca atgatacacc cacgcaatat cataatcagc
    21061 cccataatca ctgaaaagag cagtaatcag gttcaaaaac agaatactta cagcttcaaa
    21121 gtaagtggta atgcaaataa aatcgaaatc aagaaagcga tcgaacgcat ttttgatgtt
    21181 aaagttctat cggttaatac aatatgctat cagggaaagc ccaaaagctt aggtaagtat
    21241 aatggcaagc gtcctgattg gaaaaaggca atagtaaccc tccgtcaagg tgataaaatt
    21301 gccgattttg aggtttaaag aggtataaaa tgggaattaa gaaatataga cctactactt
    21361 ccactctccg ttttcgcact ggctatacct ttgaagaaat aactacagac aaaccggaaa
    21421 agtcattatt aaaatcgaag cccaaaactg gtggcagaaa taactccggt agaataacct
    21481 gtagacatat gggtggtggt catagacacc attatcggat aattgatttt aaacgcgata
    21541 aaattggaat tccagcaaaa gtagcttcca ttgaatatga ccctaaccgt acagcgcgta
    21601 tagctttgct tcattatgtt gatggagaaa aaagatatat tctcgcccct gaaggtttgg
    21661 aagttggggc aaaagtgatg agcggccctg atgctgaaat tactgttggc aatgctatcc
    21721 ccttagaaag aataccattg ggtagcgttg ttcacaacat tgaactaaaa agaggtaagg
    21781 gtgggcagat tgctcgtagc gcaggaacct atgcacaggt tgtagctaaa gatggtgatt
    21841 atgtccatgt taaaatgcct tctaatgatg ttcatttaat tcgtaaggaa tgccttgcca
    21901 caattggaag cgtaagcaat ccagatcatt ctttaatcaa gataggaaaa gctggtcgta
    21961 aacgctggat gggaattcgt ccctcggttc gaggtgttgc aatgaatcct gttgatcatc
    22021 ctatgggtgg aggagaagga aagacctccg gcggtggaca tcctgtttct ccttggggaa
    22081 aacctgccaa aggtggcaaa acaagaaaga cccgcaaata tagcgataaa tatatcgtga
    22141 aagcggttaa aaagagatag tgtgaggatt atagaaaatg gctcgttcaa ttaaaaaagg
    22201 cccctttatt gatgctcatc tgctgaagaa agtggaagtg ctgaatgctg aaggaaagaa
    22261 aagtgtaatt aaaacttggt ctcgccgttc aacaattact cctctgttta ttggtcatac
    22321 ttttagtgtg cataacgggc ataaatttgt tcccgtttac ataaccgaaa atatggtagg
    22381 tcataaatta ggtgaatttt cccctacccg cacctaccgt ggccataaag agaagaagaa
    22441 aaaagtgacg agataggaga gaaaaatgga agcaacagca acccttcgtt atgcacgcgg
    22501 ctcagctcgt aaggctcgtt tagttttgga tacaattcgc tataaaaaag taaccgaagc
    22561 acagaatata ttgcgttttt ctaatcgcag agctgctgca actataaata aattattggc
    22621 ttccgccatt gctaatgccc aaaataagga tcctaaagtt aatctggatc agttgtatgt
    22681 aacattagca atagctgatg agggtcctca aatgaaacgc tatatgcccc gtgctcaggg
    22741 tagagctttt atgatcagga aacagacctg tcatatctcc ctggaaatac agaccataga
    22801 ataataggag gaagaccttg ggacaaaaga tacatcccgt tctttaccgt atcggtgtta
    22861 ataaagatac tgattccatc tggtttgcag aaggaagttc gtatgtggat tttctccagg
    22921 aagatattaa gcttcggaat tatatccaaa aacgccttag cgataagatg gtttcaaaag
    22981 ttcaaatagc tcgtaaaaca aattccatag ttattgatat tcacactgcc cgtcctggtg
    23041 ttgtgattgg caagaaaggt gaggatatcg aaaaattaag aacagaattg aatgttttaa
    23101 taaacaaaaa tcgcagcaat ccgcttagtg tgtctataaa tgttgtaccg gtggaaaaga
    23161 tgtggctgga agcccatttg gcagctcaag aaatagcgcg tcaattagaa gaacgcgtat
    23221 ccttccgtcg ggcaatgaaa atggctatcc gctatgttat gaaagaaggc gctcaaggcg
    23281 ttaaagttca agtttctggc cgtttaggtg gagcggaaat agcgcgtaca gaaagatata
    23341 aacaaggcag aacccctctt catactttgc gtgctgatat agattatgcc aatgtagtgg
    23401 ctaatactac ttatggtgtt atcggtatca aagtatggat atacaagggt gatatactta
    23461 attaaaggag ataagcaatg ttagccccca aaaaagtaaa acatcgtaag atgatgaagg
    23521 gaagaaggaa aggtctatct tggactggat gcaatgtctc ctttggcgat tatggactaa
    23581 tagctcttga ggattcattc atttctagcc gtcaaatcga agctgctcgt atagctatta
    23641 ctcgccatat gaaacgtgta ggtaaggttt ggattagaat atttcccgat aaaccaataa
    23701 cccaaaaacc ggctgaaacc cgtatgggaa aaggcaaagg tgcccctgaa tattgggtag
    23761 ctgtagttcg tccgggcaga gttctttttg aaatcgaagg tgtggatgca aaagttgcca
    23821 aagaagcatt acgtttagca gcgcataaac tgccgatcaa aactcgcttt atcacacgcg
    23881 aaggagtgga attatgaaag cagacgaact gcgtgactta agtattgatg aattacaggt
    23941 aaagctggaa gaacttcgta tcgagctatt taaccttcgc tttcaaaagg caaaaaatct
    24001 gctcgaccgt cctgaccgta ttagaaatgt ccgtcatagt atagccagga tttacactat
    24061 tatgaaagaa aaagagaaaa aggactgagg tgctaagtgg caacaacaca taaaatgatt
    24121 aaacaaggaa ttgtcgttag cgataaaaac gataaaacca ttgtagtgcg tgtagagcgt
    24181 caattcattc atccactgta taaaaagacc gtccggcgcc ataagaaatt tatggctcac
    24241 gatgcaaata atgcagctca tataggtgat gtagttcaga ttgtggaatc ccgtcctctg
    24301 agcgcaagaa aacgttgggc tcttcataaa atcgtggaaa gaagtaaata aagaggatat
    24361 tttaaaatga tacaagcaca aacaatgctc aatattgctg ataactccgg tgccaagaaa
    24421 gctatgtgca tcaaggtttt aggtggaacg aagcgtaaat atgcatcgat cggtgatgta
    24481 atagtagtag cgattaaaag tgccactcct ggcggcagag taaaaaaggg aagtgtggaa
    24541 aaagctgtaa tcgttcgcac ccggaaagag atacggcgtc cagatggctc ctatattcgt
    24601 tttgctgata atgcagcagt tattatagat gaaaaatatg aacctaaagg gactcgtata
    24661 tttggaccgg ttgctcgtga acttcgtgaa gcgcattata tgaagatagt atccctggct
    24721 ccggaagttc tttaggagga aatgtggcaa agaataaatt gcattttaaa aaaggcgatc
    24781 tcgtgaaagt aatctccggt gacgatagag gtaaaaaagg ccatattctt aaggtctttc
    24841 ctaaaaccgg aaaggtcatt gtagagaaag ttcatttgat aaaaaaacac tctaaaccca
    24901 ctcagcaaaa tcctcaggga ggcataataa cgatggaggc acctataaac gcttccaatg
    24961 tgatgctttt caatgagaag ttgaatgctg tttctaaacc tgtatatcag attcgcgaag
    25021 gccgccgcat ccgtgtctgt aaaaagacag gtgatgagat atagaggaga tgaataaatg
    25081 aaccgtttga aagaacgcta taaaactcag gtggtagaag ctttaacaaa gagatttggt
    25141 taccacaatc cccatcaggt tcccaaaatg gaaaagattg tagttagtat gggggtaggg
    25201 caagcaactc agaataaagc tcttttagat aatgctgtaa aggatatgga acttatttgt
    25261 gggcgtaaac ctgttgtaac taaagctcgt aaatctatct ctaattttaa gctgcgtaaa
    25321 gggatgccaa ttggttgcaa agtaaccctt agaaacgaag ttatgtatga attttacgat
    25381 cgtctcatat ctttagcaat acccagaata cgagatttta gaggtatacc ggtagatgcc
    25441 tttgatggca gaggaaattt ctcctttggc ttaaaggaac aaactgtgtt tccagaaatt
    25501 gattatgata agatagatac tacccgtgga ttaaatatca cgatagtaac cacagcaaaa
    25561 acagatgagg aatgccgagc tctgttacaa gagatgggta tgccttttca aaaaagcgaa
    25621 taaggaggat tagtggccaa aaaatcactc atcataaaac aacaacgaac tcctaaattc
    25681 aaagttagaa aatataaccg ttgcaagatt tgcggacgcc cacgcgccta tatgcgcgat
    25741 tttggaatgt gccgtttatg tttccgcaaa tatgcttcgg aaggccagat tcctggaata
    25801 accagaagta gctggtaata aaatggagga tataaatgag tattagtgat ccgatagctg
    25861 atgcattgac aaaaattcgt aatgcatatc gtgccggaca ttcgcaggtg attgttaacc
    25921 ataataaact cgtggaagca ctggttaata tccttgccga agaaaacttt gttaatagtg
    25981 tccaagttct tgatagagac cccgaaaatc acattaatta caagcgtatt ttagtaattc
    26041 tacgctatac taatgacggg gagccagtgt tgcaaggaat tcagagaatt tccaaacccg
    26101 gaagacgggt ttatgtaaaa gccgataatt tgccctgcgt ctataatcat actggttgtg
    26161 ctattatttc aaccagcgaa ggtgtgatgg tagatcgcga tgcgcgcctg aaacgtgttg
    26221 gtggcgaata tgtttgcaag gtttggtgag ggggaaaaag atgtcacgca ttggaaaagc
    26281 tcctattaaa ttaaactctg aaacaaaagt gaatgtaact aataatgtga ttactgttaa
    26341 aggtaaattg ggagaactga agtggacact tcttcccgga attactattg aaattgaaga
    26401 tagtgtttta agagtaaatc gtgctgatga tagtaaagat caacgtgctt tacatggttt
    26461 aactcgtgcc cttttacaaa atatggttac tggtgtaacg gaagggtatc aaaaaactct
    26521 gcatatatat ggaactggtt attcttcgga agtagtagga ccttggctaa gactttccct
    26581 tggtttttca catgatatcc tattgcagat tccaaaagag ttacaagtga gtgcagaagc
    26641 cgtcccacgc tccaagggta ctcgatcaga ttttcaaagt ataataacta ttaaaggtat
    26701 agacaaacag ctggttggtc agtttgctgc agaagtacga aattgccgtc ctccagaaaa
    26761 ttataaaggc aaaggcatac gctatattga tgaacatgtt attataaaag ctggaaaagc
    26821 cgggactaaa taaggaaagg tgaaaagatt atgataactc caagctgtaa gattaaaaat
    26881 gaccttcgga aacgccgtca tttctctatc cggaaaagag ttagcggaac atcagaacgt
    26941 cctcgcttag tagttttccg ttctctgaag catatatatg cacaggttat agatgatacg
    27001 aaaggtgaaa ctttgatctc gatgtctact atagccaaag atatgggtac tttaactggg
    27061 aagaaaaaaa ctgaacaaag ctacgaagtg ggagtaaaac ttggtgaaaa agcacttgct
    27121 gccggaatta aaaaagtagc ttttgatcgc tccggttata aatatcatgg aagaataaaa
    27181 gcccttgcag aaggtgctcg taaagccgga ttggatttct aaggagcaga gatgaattac
    27241 gaacaaaatc atatggaaga agaaaaatta attgaaaaga taatcgaaac caagcgtgtg
    27301 gccaaagttg tgaaaggtgg cagaaacttc agcttttccg ctattgtagt tgttggcgat
    27361 aaacaaggca acattggtgt aggaaatggt aaagcgaacg aaatcgtgga tgcaattcgc
    27421 aaagcgaaag aaagagccgt aaaaaatatg ttcaaagtgc ctattgtgag agggactgtt
    27481 cctcacgaaa tcatagctcg ctacggagca tctcgtgtaa tgataaaacc tgctgctcct
    27541 ggaactggaa ttattgcggg tggaactact cgagccattt ttgaggccgc gggaattgaa
    27601 aatattttgt gtaaaacact tggctctaat acccccacta atgttgttaa agctacaatt
    27661 aatggcttaa aatcgatgcg tacattgtcc gatatttctc gcttaagaaa taagacaatg
    27721 gctcaactta ctggtcagga ggagaaatga aactgaaagt tactcaaatc cgcagtacaa
    27781 taaatcgcat cgaagaccat aaaagggtta taaaagctct cggtcttggg agaataggga
    27841 aaagcagaat tcataatgat agccccacta tccgaggtat gatcaacaag gtcggttacc
    27901 tcttaaaagt tgaagaactg gaggaggaat aacgatgtta acattatccg atttacaaag
    27961 atcagcagga agaaaagata aaaaacgcct aggaaaagga caaggttctg gttggggaca
    28021 ccaagcaggt cgtggacaca aagggaaaaa agcccgtgca ggtggaaatg tacctcctta
    28081 ttttgaaggt ggacagatgc ctatgaatcg tcgtcttccc aaacgcggtt ttaaaaatat
    28141 cttccgggaa aattaccgca ctttgaatct gtatcgttta cagtatatcg atattacaga
    28201 tctggatatt ccaactatgg aaaagatggg attaattccc tccaaaggga aaaaagccaa
    28261 tgcgccggtg aaagttttag ccggtgtgaa agatgaattc accaaaaccg ttaatatcaa
    28321 agctaatgct ttttcacaaa aagccaaaga gattattgag aagaacggtg gtaaggcgga
    28381 ggtagtgtaa cttgttcaag actattggta atatgttccg gattccggac ctcaagaaga
    28441 agatcttttt tactggcctg tttctggtgt tataccggtt aggaagtttt attccacttc
    28501 caggagttaa cgccacagct ttgaaagctt tttttgaagg tgctcgtgaa ggtggcaata
    28561 cccttttcgg gcttttagac ctctttgtgg gtggtaactt tgaacgtgcg tcagtttttg
    28621 ccttaggtat tatgccttat attactgcgt ccattgttat tcagctattg gggagtatta
    28681 ttccctattt tgaaaagctg cgtaaggaag gagccgaagg ccaaaaaaag ctaaatcaga
    28741 ttacccgtta tggaaccgtt attttagctg cttttaacgc tataacaatc acaatgtggc
    28801 ttactaatct ctctggagga gtagttccgc gcccgaattt tttgttccat ttaacaggga
    28861 taataacttt gattacggga acaatgattg ttatgtggat tggggaacaa attaccgaac
    28921 atggtatcgg caatgggata tcgctgatca tttttgccgg cataattgcc aggtaccctg
    28981 aaggttttgt aagaatgttt caaaaaatca gtgttgattc cagctacacc tggaaagctc
    29041 tggtaaccat tattttaatg gtgttggtta ctgcagcagt aatttttgta acggaagctg
    29101 tgaggaaaat tccagttcaa tatgcaaagc gtattgtggg gagaagagtt tacggtggtc
    29161 aaagtactta tatcccatta agggtaaata ctgctggtgt tattccgata atttttgcgc
    29221 agagcgtttt aatgtttccg gcaacgatta ttgcacttgt gggtagtaac agcggattct
    29281 gggtaacttt gaaaaacttg ttatcaccag gttcagtgct ctatacaata ttatatgtag
    29341 ggctcattgt tttctttgcc tatttttata cagccattgt tctcaatcct acagaaatgg
    29401 cagaaaatat ggtaaaatat ggtggtcata ttccaggaaa gaaacccggg aaaaagactg
    29461 cagaatatat taacaatgtt ctggttcgca taacccttcc tggagctatt ttctttgcat
    29521 ttattgcttt actacctgaa attatgagta accaattaga cctacctttt tattttggag
    29581 gtactggtct tttgattgtg gtaggtgtag ctttggatac tttgcagcag attgaatctc
    29641 atcttgtgat gcgccattat gatggtttta tgaaaaaagg caaattacgc ggacgttcaa
    29701 tgtaatatga tatacataaa atctgcttca gaaatagaaa aaatgagaaa aagctgccac
    29761 atagttggtg gcttactgga tgctttggaa ggtcttatca ggcctggaat tagcacctta
    29821 gaattggaca aatttgctga ggattatatt ttgagcagag gcggcaagcc ctcttttaaa
    29881 ggatatcagg tagatgggct accacctttt tcaggttcaa tttgtacctc aattaatgaa
    29941 cagattgtcc atggaatacc aaatgccaat agtatcctta aagaaggaga tattattggg
    30001 gttgatgtag gtgttaattt aaatggttat aatgctgatg cagcaagaac ttatagagtt
    30061 ggcaaaatat ctagcgaagc ggaaaagctg ttgcaggtaa ctaaagaatc tctgtataga
    30121 ggaattgcag cagccatccc tggaaaccgc attggagata tttcttatgc gattggctcc
    30181 tttgttaaat ccaagggcta ttttgtggcc gataatctta ccggacatgg cattggacgc
    30241 aatttacacg aagagccaat gataccaaat atggggaaac cgggaagagg acctcgtttg
    30301 tcttccggta tgactttggc tattgaacca atggttaata ttggaacgaa tcgtgttttc
    30361 gagaatgggt gggagttcaa agtttgggat aacagcttat cagctcattt tgaacatact
    30421 atcttaataa ccgatggcga acctgagatt ttaacagttg ctgagtgagg ataatttgct
    30481 atgagtaaat ctggtgttat agaagtggaa ggtattgtaa ctgaagctct tcctaacaca
    30541 acttttcgcg ttactctgga aaatggccat gagatattgg ctcattcttc agggaaaatg
    30601 cggatgaact atatcagaat tctgccggga gataaggtaa aagtggaact ttctccctac
    30661 gatcttaccc gaggcagaat aacctaccgc tataaataaa taataaatga ttgcaataag
    30721 ccctgtggct aagcaaggag atgataatga aagtgaaagc ttccgttaaa aaaatctgca
    30781 aggattgcag aataattaag cgcaacggta taatccgtgt gatttgcagt tcaaacccca
    30841 aacataaaca gagacagggt taaggaggga aaacttggca catatagcag gtattgaact
    30901 tccaaagaac aaaagattgt ttattgggtt aacttatatc tatggtattg ggcgtaccaa
    30961 tgccaaagaa atctgcaaaa aagcaaatat agacgaaatg aaaaaagtag ctgacttaac
    31021 gatggaagag gaaaaaatcc tgagggacat cgttcagaat gagtatgttg tagaaggtgc
    31081 attacgaacc caaatagcta tgaatatcaa acgtctgatg gaaattggtt gttatcgcgg
    31141 tctccgtcat aaacggggac ttcctgtccg cggacaaaga acccatacta atgccagaac
    31201 ccgtaaagga cctcgtggca gttcaattaa gaagaagaaa taggagaaga tatggctaca
    31261 aaaaccagaa ccaagaaaaa acgcgtgcgt cttgcctttg atgaaggtct cttctatgtc
    31321 cattccagtt tcaataatac tattgttact ttaacagata gagctggaaa tgttcttgcc
    31381 tggtccagcg gtggaaaagt tggcaacaaa ggctctcgca aaagtactcc ttttgcagct
    31441 caagttgctg cctcagaagt tgctaaagca ggtttggata tgggaattca aaaagttggc
    31501 gtgattgtta aaggtccagg tggaggacgc gaatctgcca tacgggcagt taatgcagca
    31561 ggaatgaaag taacaatgat taaggatgaa actcccattc cgcataatgg ctgtcgtccc
    31621 cctaaaacca gaaggatata aggagataat caatggctaa atatacagga cccaaagcaa
    31681 gattatgccg taaattcgga gaaaatattt atggtaatgc taagtacgat aaaatactta
    31741 gcaggcggaa atttcctccc ggacagcatg gtagaacttt acgaaggaaa cctactgatt
    31801 ataatttaca cttgcgtgaa aaacaaaaac tacgcaacac ttattgcttg ttagaaaaac
    31861 agttcagcaa ctatttcaaa aaagcggcta aagcttctgg cgtaacaggt gataatttat
    31921 tacagctctt agaacgccgt ttggataatg ttgtttatcg gatgggtttt gctgtaacga
    31981 ggatgcaggc acgtcagttt gtaaatcatg gtcatttttt agtaaatggc agaaaagttg
    32041 atattccttc ctatcttctt aaggaaggag acattattga agtgcgcacc aaaagccgca
    32101 gtatgaaagc gcttgtggaa gcaatggata aaaccgaagt aacatctcca tattcttggt
    32161 tgagtataga taaagaaaat ttccgcggac aatttaattc cattcctgcg gtaagtgaga
    32221 taccggtcac ttgtgatctg cgtctcgttg ttgaattcta ctccaagtaa tgcgcgataa
    32281 gcttaaggag cagctatgat gtatcttgaa cctttacaga tgccggaaac ggtagattac
    32341 gataaagaaa cttattctcg aactttcggc aagtttgaaa tcggtccttt ggaaccaggt
    32401 tttggaacca ctctggggaa cactttacgc cgaattttgc tttcttccat tcagggagcg
    32461 gcagttcgtt ttgtaaggat agagggtttg caccatgaat tctgtcccat tccaggaacg
    32521 aattctgatt atatagattt aatcctccgt attaagcagc tcgttatcca atccagttcc
    32581 atagaagaag ttccaattgt tctggaacac aaagggaagg gtgtgataac tgcttcaatg
    32641 attcaggaaa ccccttctgt taaaattatt aataaagacc tatacctgtt ggaagtgatg
    32701 gaggatgtag attttcgttt ggaaatgatt gtaggtatag gacgtggtta tgtttcagca
    32761 gatagacaaa ataccgaagg taaagcagag ggctttatcc cgattgattc tatctattct
    32821 cctgttctga aagttaattt cagcgtgtct catcagcggg ttaaagagag aatgaatttc
    32881 gatcgtttga ttatggaaat tttttccaat ggtgctattg agccctcgat tgctctgttt
    32941 cttgcagcta aaatactgaa agatatggca gctaagatat ctttgtttga aactgaacct
    33001 cagtacattc gcgatgtaga attagatcca gacttggaag agaaagaacg catcttgcaa
    33061 accagtgttc gcgaaattga acttagcgtt cgtgcagcta actgtttagc ccaagctaat
    33121 attgaaacca ttggtgattt agtttcaaaa tcggaagcag aaatgctcaa attccgtaat
    33181 tttggcaaaa aatccctgga agaaatcgta caaaaattaa aaaaatacga actgcacctc
    33241 gggatggatg tggaaggtat ctatcgtcaa attagagaag cccgtagccg cggagttctg
    33301 cctccagaag aaaaagaaat tcctattcca gaagccaaac ctaaagctaa acctaaaacc
    33361 aaaacagctg ccgaaaaagt taacactgtt gaaaagaaga cttcagaaac agctactgct
    33421 acaccaccac ataaaccagt gccaagccca aaaaagaagg tgaaaaatga gacatagagt
    33481 tgaaggaaga aaatttggac gggaaatgga tgccagacgt ctgatgatga gaaatcttgt
    33541 taaatcaatg atagaacacg gtcaactgaa aactaccctc cccaaagcaa aagaaatgag
    33601 aggatatgtt gaacgcatca ttacctacgg taaaaacgat accgtccatt caagaagatt
    33661 agcatatagt gtcctgggcg atagaacctt agtgaaaaaa gtgtttgatg aaatcgctcc
    33721 agcttttgaa ggtcgcaacg gtggatatac aagagtgtta aaagccggtt tccgaaaagg
    33781 agataacgcc ccgatggcta ttatccagtt cgttgaagaa tccactatca aacctaaaaa
    33841 gaacgctatc aaagccaaag acctgaataa ataattgttc tggcataaat atctggattt
    33901 caccgattaa taattatatt aattggctat attagtaata gcgaagaaat ccttattgtt
    33961 taaacggttg ccgaattctg attcccaaaa ttgctaatag cttttcaaga atcggatttc
    34021 ggcaaccttt ctttgtaaat gcttcaatac atcccgtcct catcccgtcc ttatcccgtc
    34081 ctcatcccgt cttcaaccaa tggttttgga tgagcggatg aggaattaac ctcaagtagt
    34141 tgatatgttg tgttctagta tatgctatgt gctttcttcc ggattttaca gttaaaatac
    34201 cagagggttg atgtcctcat cagccataaa tatagaaagc ttacgataaa caggagtaca
    34261 ttagtagagg gcagagagct ttgggcaaga gaaaaagtga gcctggaatg tagaggatat
    34321 cgaaaataat cccagcagga cgatagattt gagcgacggt ttttaccccg atgaagaaaa
    34381 tgcgagacaa acaattgcct cgcatatatt taaaagacat tttgaaatta ccgccaaaat
    34441 aactttgaac taagaactcc tgaactttga actaagaact cctgaacttt gaactaagaa
    34501 ctcctgaact ttgaactaag aactcctgca ccgataatca atgctcagtt ctttttattt
    34561 tatttcccgg ctgcgataga aatcattttt cccggaacaa caatcacttt ctttatatta
    34621 agtccatcca gataacgctt aacattttca ttttccaatg ctttagcttg caattcgtct
    34681 tggttgatat ctgctaaagc ttccagtttt cctctaattt ttccgttaac ttgaataaca
    34741 taggtaacaa tgttccgagt taaatattgt tcttcatatt ctggtaagcc acaatcattc
    34801 agcatatttt catttcccaa tttttgccaa agctcttctg caatatgagg cgcaaaagga
    34861 tagagcattt taggcaaagt tatacaggca aacgcataaa tctgctgttc ggcggcattc
    34921 aattttccag gatccttaat tttaacgata gcattcaaaa attccataca agtggcaatg
    34981 gccgtgttga attgcaaacg ctgtaaacaa tcctctcgcc atctataaat ggcataatgg
    35041 gtctcataac gaagctcttt tatttcttca gagatattat cagagcattc agttgactgc
    35101 aacccacatt taatcacttc cagattacta tctatcaaac gccaaaccct gttcaaaaat
    35161 cggaaagcac ccataatacc atcatcactc cattccacat ctttttctgg aggcgaggca
    35221 aaaagcaaaa aagtgcgtaa agtatctgct ccaaagcgat ctataatata ttttggatca
    35281 actacattgc ctttggactt gctcattttg gctccgtcct tagttaccat tccttgagtc
    35341 aataaacgcg caaagggttc atcacaattc acccaaccca aatctctcat aaccttatgg
    35401 aaaaagcgag cataaagcaa atgcatacag gcatgttcaa ttccgccaat gtattgatca
    35461 accggcaacc agtaattagc cgactcccta gaaaaaggtt gtttatcatt tttaggatct
    35521 gtataacgag cataatacca actgctatct acaaaagtat ccattgtgtc tgtttctctt
    35581 tttgcgggag ctccacattt tgggcaggga acatttatcc attctggaac actcaaaagc
    35641 ggatttctgg tagttctgcc tacctgaaca ttatctggta atttaacagg caaatcctca
    35701 tccggaacta aaactatccc acaatgagga caatgaataa ccggaatagg atttccccaa
    35761 tagcgctgac gcgaaataca ccaatccctt aaacgataac tgacggtttt ttcgccccag
    35821 ccattttcca cagcccaatt agtgattgca gaaattgcct cctcgctatc catcccgttg
    35881 aacttatcag aatttaccat aattcccgac tctatatatg cttcctgcat ttcttccaat
    35941 tttaaggaca tatctggttt ttgaatcacg attttaatcg gaatatcgta ttttttagca
    36001 aattcataat cgcgttgatc atgtgccggg acagccataa ctgctcctgt tccataatcc
    36061 attagaacat aattagttat ccaaatttga actttattac cattaaaagg atttatagca
    36121 tagcgcccgc tgaaaatacc ttctttgatt gtttctgcgg aactgcgcaa aactttgtct
    36181 tcattgatca ctttgtggca aaaatcctgc aattctttat tatccggttc ttcttttaac
    36241 catttttgca ctaaaggatg ctccggaggc aaagccataa aggttactcc gtaaatagta
    36301 tcaggacggg ttgtaaaaat cggtatattt tcgtttgtcc cctctacaat aaaatcaact
    36361 ttggcacctt cggaacgacc tatccaattt ttctgcattg taatcactcg ctcaggccaa
    36421 tcaatcactt gggaaaaatc cagtaattct tcggcatatt cagtaatttt aaaataccac
    36481 tgttccaagt ccttttgttc tacaatgttt ccacaacgcc aacagtgacc atcctcgact
    36541 tgttcgttgg caaggactgt ctgacatttt tcacaccagt tctgaaatcc tttttttctg
    36601 taaactaagc ccttctcata taacttcttg aaaagatatt gaccccaacg ataatattct
    36661 ggacgacaag tgctgatttc tctgctccag tcgaaaccaa aacccatact gtcaaattga
    36721 atgcgcattg ctgcaatgtt ctcttccgtt gtaagacgcg gatgggaatt gtgttcaatc
    36781 gcataatttt ccgcaggcat accaaaagca tcataaccta tgggctgcat tacattgaaa
    36841 ccttccatca ttttcaatcg cgaaatcaca tctccaataa aataattgga agcatgccct
    36901 acatgcagaa ctcccgatgc ataaggaaac atagaaagaa cataatattt aggtttggaa
    36961 cttgtattgg gagcattaaa tattcctttt tctcgccaaa cattctgcca tttcttttca
    37021 atttcgttaa acctgtattc cattttgtat tttcctgcct atcattttat cttttaacac
    37081 tttgcaaaat ctgttagagc tcataattgt caagcaaaag ctgcaattta gatttttact
    37141 cgcttttttc cccttgtata aaccctggat ggtgataaat taggatggct aagggatgag
    37201 aagaaaggtg cttgacaatc tggatgataa taaaaaagga taattttgtc taaatagtta
    37261 ggagttgaaa gatggatatg tccagtacaa gtataaaccc tcaccagaaa cttcaatttt
    37321 aatcacgaaa acttcaaatc gaggaa
//
LOCUS       DAOSTH010000012        37041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_1253_length_37041_cov_14.301438, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000012 DAOSTH010000000
VERSION     DAOSTH010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 37041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37041
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1253_length_37041_cov_14.301438"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="PLB65_03325"
     CDS             complement(<1..502)
                     /locus_tag="PLB65_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOQ77588.1"
                     /translation="MIEVLIDKCVGCGSCLSACAYDAIKIEDQIANIDPDKCVLCGAC
                     VSACPFEAILLRKAKAEAIDKSEFNGVWVFAEQRDSIIAPVVFELLGKGRELADELGS
                     ELSAVVLGHQIESLSSDLIAFGADQVLEVDDPTLKHFRDEFYAKALTELALKYKPSVT
                     LAGATVI"
     gene            complement(1151..1882)
                     /locus_tag="PLB65_03330"
     CDS             complement(1151..1882)
                     /locus_tag="PLB65_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77589.1"
                     /translation="MKTFKFVLVSLCIMSMLTSWVSIEAKKKEEDKGKEVESKLFLDD
                     SKLDNQDYYNQFPSSAIQSFENALLTPKVSDIKIITINSDQTKTDAKIAEEMIAVALG
                     MRQEYVNLEAEGKSLLSGNLEGSELEISEMVKAKLEDLKTAKAQLSESQKVYFLSGFT
                     YLASSIVREKVILESAKMYVDEASKATGMAKMKYAKDLPYATAVVTDIPILLESQIKT
                     INEFATIAKTQSIKIPENVTKSLFE"
     gene            complement(2047..2361)
                     /locus_tag="PLB65_03335"
     CDS             complement(2047..2361)
                     /locus_tag="PLB65_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77590.1"
                     /translation="MIITFSVQNFFPLRNKVSLDFRTTSDKTLEDYYVVSTEKPKLRI
                     LRMAMIYCADASGKTNLLMILDFIKRFTSNTSINKELPILITPFALDKDKQLNFSSGE
                     EV"
     gene            2823..3881
                     /locus_tag="PLB65_03340"
     CDS             2823..3881
                     /locus_tag="PLB65_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOQ77591.1"
                     /translation="MKSNGCRYGTHRVIEPKGVLPQPAKVLNNDMSEIWDNEMLIDVI
                     RLNIDSASFHQIKNKLIAKGFTDLEKAFAEHAIDLLNRTGKHKNEDTGSGGMLIGRVA
                     AIGDKFEMKEEVKVGDKIASLVSLSLTPLKINKVKKVLLDKDQMEIEGQAILFSSGIY
                     AKLPDDLDENLALSVLDVAGAPAQVERLVKPGDTVVIIGANGKSGILCNAVAKERAGI
                     CGKVIGVVRKESYIPTCKTTGCDEVILASATDAITIQKEVSRLTNGKMADVVINVVNT
                     EDTELPSIMAARERGLVYFFSMATSFTKAALGAEGIGADVDMMIGNGYAKHHSEIALD
                     LLRRNPVLMKIFKERYAE"
     gene            3896..5146
                     /gene="ablA"
                     /locus_tag="PLB65_03345"
     CDS             3896..5146
                     /gene="ablA"
                     /locus_tag="PLB65_03345"
                     /EC_number="5.4.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050066 - lysine 2,3-aminomutase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0046440 - L-lysine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HOQ77592.1"
                     /translation="MSKIIDLKNTATPDEWNDWHWQIKNRITSYEQLAKYIELQPEEE
                     AVFKDKAFSFRMAITPHYLSLIDQSNPYDPIRLQAIPRIAESHISSSDMADPLSEDAD
                     APVPGMTHRYPDRVLLLLTDQCAMYCRHCTRRRKAGEYDAPMPKENVDKALDYIKEHK
                     EVRDVILSGGDPLTLSDERLDDILNKLSKIEHIEIVRLGTRTPVVLPQRFTDSLLTIL
                     KKYKFVWLNTHFNHPNELSEDSCKALAKIAETGIPMGNQSVLLKGINDNVDVMKALVH
                     KLVKNRVRPYYIYQCDLSEGISHFRTPISKGIEIIESLRGHTSGLCIPTYVVDAPGGG
                     GKIPVMPNYVISQMPGRVILRNYEGFITSYTEPEFAIQDESKYRELCADEYKSTEGIM
                     SLMRGKKVAIGPAGTKRHQRRNHQ"
     gene            5155..6150
                     /locus_tag="PLB65_03350"
     CDS             5155..6150
                     /locus_tag="PLB65_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77593.1"
                     /translation="MEFSWISAKRCINIAIVGICKNAGKTTVLNYIVENYPHKWGILS
                     TGRDGETEDTLYKTPKPKVKLPAGCLFCADAKTLVQQGSGISILAKTKWQSGGRELWI
                     AKSEIDLETEITGPANVNGQIACAELFKKLGAAKVLIDGSLDRKSIVLSEAIDGIILA
                     AGASFGSQQAIIDELQRLITLSQIGTYHSSTLKKLTEQNKILIKSQNRWKTTALVSLI
                     ANETKLLEFINEINNPTHLYIPGAYTSSVNNRLGKHLKGIQLVFRHPECIKLSNIELE
                     VFLEDHKPLSLIPFKLKAIALNSHGVGSNEIDADEFHHNLRSVFNNYPLLDVMEI"
     gene            6151..7095
                     /locus_tag="PLB65_03355"
     CDS             6151..7095
                     /locus_tag="PLB65_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOQ77594.1"
                     /translation="MADRDSITRVTVNLGLFSNILLSIIKTCVGIFGHSAALLADGIN
                     STSDVVYYIAVKIFMKQAQKPADVEHPYGHRQLESISAIVVGAFILTTGIAIFWESIN
                     KVYDILSKIETGHSASSWALVIAIFTFCLKIFLYTYTRKNMLKTNNPTLKALANDHLN
                     DIMASIAVIVGVLLGRLGYYWMDPAAGAIVAIYIIKTGIEIIMESSRELMDYLPDEDF
                     SREIRTEALAVEGVRSIEELGIHRFGPYYTVNMTITVDGAISVDDGNNISDKVEKRLL
                     DKFSSGLRQVHIHYHPYTEKISLSNNHPENKESQGVNI"
     gene            7092..8330
                     /locus_tag="PLB65_03360"
     CDS             7092..8330
                     /locus_tag="PLB65_03360"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ77595.1"
                     /translation="MNWGKDILFSLVLFLLASFCLLSILGINSEPLPELKLDISKPTE
                     PWIIEIIPPGGSIFSVLANQNVPPQEIGLLAYNFGDYIDVTTIQPGDTLKVLLSEDKQ
                     HIKKLMFVQEPTTRHHFTVSGDSLIYHLEALPVKKAKRIIEGTIQETLDATLLTMGFA
                     PQDKQAINNGLETEINFSRDARNGDQFLVFVEERIFEGKTLPGRKIFYVQYTGERTGT
                     CELFRYEDAEENSVLNGLYTIEGKSCHSNGVGFPLSVIHVVSSFGKRLDPFHGSWANH
                     QGVDYRAHYGTPVYAVAGGKVTTASYNGGWGNEIRINHPSGLSTQYAHLSSINVHKGQ
                     NVKKGQIIGRVGSTGRSTGAHLHFGLIKGKKYINPTNLKMVGTEKLNETQMARFKSQQ
                     QTILQEMENLKHPGPLITKK"
     assembly_gap    8832..8931
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     assembly_gap    9136..9235
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9293..10060)
                     /locus_tag="PLB65_03365"
     CDS             complement(9293..10060)
                     /locus_tag="PLB65_03365"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ77596.1"
                     /translation="MSDCILEAQDIRKSFFFKGSLITVLDNVQFHIRTGQFVSIIGPS
                     GCGKSTFLELLAGITTPDEGFILLKGNDITAKTGFLGYMPQDDLLFPWLTVMKNILLP
                     VKVTGSNEQEAKQKINELLPLFGLQNYTEHLPYQLSGGLKQRVALLRTYMCSADILLL
                     DEPLAKLDALTRSQLQSWLKDIVHLLNLTVILVTHDIDEAIMLSDRIDLMGINPGTFI
                     ESIKLDRNNPLTAEEQLKLKIKLRSQLVNGESNNDKK"
     gene            complement(10065..11072)
                     /locus_tag="PLB65_03370"
     CDS             complement(10065..11072)
                     /locus_tag="PLB65_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ77597.1"
                     /translation="MKIILNVLRIYCYLILAVTITLCLFSCKAQTPRQDEKGLQKVSI
                     TLDWTPNTNHTGIYVAKELGYFKEQSLEVDILQPGQNITDQIVATGKSEFGVSYQENV
                     IRARSENIPLVSIAAVIQHNTSGFASLKESGIKSPTDFEGKKYGSWDSPSELAILKAT
                     MQKYGADFKKVTVISGIYDFFSTIGKDADFEWIYYGWDGVEAERRGIALNYIPLKDID
                     PVFDYYTPVIISSEDFLAKNPETAQKFMSALKKGYEYCISKPDSAADILLKNVPELNA
                     EQVRRSMQYLAKEFQSDAPTWGRQKPIVWKRFCDWMYQQRLIQLAVEPNKAFTNDYLP
                     K"
     gene            complement(11276..12220)
                     /locus_tag="PLB65_03375"
     CDS             complement(11276..12220)
                     /locus_tag="PLB65_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HOQ77598.1"
                     /translation="MMSTLLKKPRTITAGIEEELSPALKSYLSSFVYHLKVERGMAKN
                     SIESYHRDIGDFLLFCPKEIGSYETSDITKYLLSLQDIGLLNSSIARKRVALGQFFGF
                     LKDNDIATKVDMDLVPRIKLGVHLPDVLSVEEMFQLLNSLPVKTPLEIRNKLMLELLY
                     ATGIRISELLDLTLHSINLKERVILVHGKGSKQRYVPYVNTLDELFEKYLKQSRPLLL
                     KLKQSEVLFLNNRGDKMSRMGFWKILRKAALEANIKKDITPHTFRHSFATHLLEGGVN
                     LRIVQALLGHSSIDTTQIYTHIDMKRMVETYKQYHPRA"
     gene            complement(12171..13628)
                     /gene="guaB"
                     /locus_tag="PLB65_03380"
     CDS             complement(12171..13628)
                     /gene="guaB"
                     /locus_tag="PLB65_03380"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOQ77599.1"
                     /translation="MNLHKAYTFDDVLLLPQQSEVLPSEVDLTTNITSNISLRIPVIS
                     AAMDTVTESAMAIAMAREGGIGIIHKNLSIEEQARQVSLVKRAESGVVTHPYILAPED
                     TLEDVIALKEAHHIGGFPVVENDFLVGILTNRDFRFETNPNKKVKEMMTPKEQLITAK
                     TGISMDSAIDLLQEHRIEKLLLISDDGKLEGMITVKDIMKRLNYPEAVQDDKNRLLVG
                     AAIGVKGDYLERAQELVKAGADLLVIDTAHGHHKNIGIALQNVKKHLNCQLIAGNVAT
                     AKACRYLIDNGADAVKVGIGPGSICTTRVIAGIGVPQLSAIMDCALEANKNSIPIIAD
                     GGIKFSGDIVKAIAGGASAVMIGSLFAGCDESPGESIIYNGRRFKSYRGMGSLSAMKL
                     GSKDRYFQDEQLEENKLVAEGIEGMVPYKGPLKDFLYQLIGGLRSGMGYCGAANLNDL
                     RARAEFIEITSAGLKESHPHDVHITKETPNYYSGD"
     gene            complement(13648..15375)
                     /locus_tag="PLB65_03385"
     CDS             complement(13648..15375)
                     /locus_tag="PLB65_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ77600.1"
                     /translation="MKKIALLLLILIACESAIYAQYDEKQILTQQANQLLIQRQYAQA
                     EQLFLQILDKYPNDLNSINQLMNLYFTLSQTDKAETTLKKYQRILPPQTYSEFYIQLL
                     VTQAKVDDAWQEAMRHLANFGYDEFKFRRLASFFERKAFYDKVMELYTMARNRLGKPD
                     LFRLEMANTALNCRQFEKAIREYMAYTESNPVYLFFTNNQLKTILQEEPSLISVVSAI
                     ADSSKSSVLKELYANALVNMKDYKTALSIYKQLDPQKMWHFAEDQAAVGNDSIAFEAY
                     SYLENIETDQVKKMDLLFRMAELKYQQRDYPGSEQIISGCLSMPFWKDRNLYYRSNVS
                     VKLRKLMAEIMLAKNEPVDSALVWLNEAKKFSRNNLEAQEMDLEIARLYILNSEKEKA
                     DNILKTINYNTLMETRSYLNFLNTFLAGETALADTLMNEFIIRYPESKYTNDAIYLMM
                     FTLNMDEQDRSTFYSAVRLLQLNHPSGIDSLEVIFKHNKDEEILLLAIEWAIAFGDET
                     RAAALLQYDFQDEIASDYAALLRLLLVADRDEEQRMAKEFLKNKPNSVFSPDFRKKIS
                     RWATSKPNI"
     gene            complement(15386..18619)
                     /locus_tag="PLB65_03390"
     CDS             complement(15386..18619)
                     /locus_tag="PLB65_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77601.1"
                     /translation="MSELTKFIQKYRQKINLRFVLRAIIGSLLSFAVALHIYFLLWLN
                     LPSQSMALLYANIGIRTCLIMVIIYFLWSGYRNFWHNLSVARFLDKQIDYRDDLFQNT
                     LELAEKEKDSPIMENLSILAEHRIKENQYKIPALYPSWIVFAILFLIVGLGSIWANSF
                     SDFRLAFKQFYTNKGQEVIYKDYIELSPGNKTIGRNQPIEIKILNPDPRLQHHLFYRI
                     DKQWRELGLADNSYIFQRLDNSIEYYAQNEVCKSPIYKIQVLDEPIVKKWFVQYNPPA
                     YSGLTSWTDTLSYGNIEALKYSKVKLSFSTNIPVESAVMVFDDASRLPLQALDKSNYI
                     VQLSIEKPRTWYLELTDALGRQNKPEEKTISILEDNPPEIRITFPGEDVNLNQNMLLP
                     LIIEADDDYGLKNCALNIQIMASEPQVLNVQSVISGKMFTTDFLLDLKEANLFPGDVV
                     TYWATIYDNSPEQHKAESTRFKARFPSIEEIYREIETKEHEKKSELQTALEKSKDLQK
                     EFENKRRELLKKENLQWEDKKQLEQILQKQENLSEQVQNIADNYQELINKMQANETLS
                     PETLQKMQKIQELMQEINNEELQEAMRKFENALQNVKTEDLKKALENFKFSMEDFAKK
                     IDQTLALLESIKKEQALQKALQISEEMEKMQKTLSERTMDGKQNPKDLAADQKQISDT
                     YDKLQQELSKISEMLESPRDNDVIKQLNDLMQDMKNSNLKQDFQNSQNALNQNQRSES
                     QSAQNNALEKMRRFSLKLNKMKESMGGGNQQRMLQAVQTAIRELLIFSKQHEATTACY
                     RNDPYAIIHDLISQSEGIQISLNKLFSETQVLMMIPPKFFIDLNDTNNAYRNVFSSIN
                     ESQFYQIPNNLTFIQKGINLMVYDLMQALQNASSGSGSGGGMQSLLQMLEQMSQEQMA
                     MNMLTEQLMLQLQAQGGKMDSAMQQQIQRLAADQERLAENLKRALQNNPEAQKQGNVI
                     KQITEEMEAITRQLKNNQLNPELLERQERIISKMLDAQRSINKREFTEKRKAEAGQDI
                     PILGNTKIDLEALRRSGLLDEGLRAYPKEYQQVIMQYLKALNEATSK"
     gene            complement(19169..19702)
                     /locus_tag="PLB65_03395"
     CDS             complement(19169..19702)
                     /locus_tag="PLB65_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOQ77602.1"
                     /translation="MRWMLILFLTLAFTVLGCTPTDQPVSSEDVLNNPDKYASQEVEI
                     EVKPGEWLTDWDQAIAVAKKQKLPILVDFTGSDWCIWCKKLDSEVFSKEEFINYAKKN
                     LVLLKLDFPHGIPQSDAMKAANKKKMNEFNIEGFPTIVLVNAEGKEIARTGYRPGGAE
                     SYINHLQDLLTKQNDIK"
     gene            complement(19699..20130)
                     /locus_tag="PLB65_03400"
     CDS             complement(19699..20130)
                     /locus_tag="PLB65_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOQ77603.1"
                     /translation="MTTIVQTHWSGEMKFTACVDGHEIVMDADPEFGGSNEGPRPKPL
                     LLAALTGCAGMDIVSLLEKMKVKDYIFSMDAQAKTSSEHPVTYQTITLFFNFQGKELP
                     PDKIRKAVNLSLERYCGVYAMLKKAANVIAKITINNQEIEL"
     gene            complement(20131..20586)
                     /locus_tag="PLB65_03405"
     CDS             complement(20131..20586)
                     /locus_tag="PLB65_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HOQ77604.1"
                     /translation="MADYANKFHDLITRLQMVLSEIDYAQKACLQAGRMECMLLNYLY
                     KTNGPANMNELAKVLNVSHSRVTRIMDNLVNKQLVIRRPSEEDRRCWYAQITDKGKKL
                     AANSLQTVLDQQEKILEKLPEKDVDTVYKALKQYVDKYEEVLKETLEGI"
     gene            21054..22388
                     /gene="radA"
                     /locus_tag="PLB65_03410"
     CDS             21054..22388
                     /gene="radA"
                     /locus_tag="PLB65_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOQ77605.1"
                     /translation="MATIFFCTDCGYETIKWSGKCPVCGAWGTMKESTRIAGKNNKLG
                     ETITQPKPERIIDLKYDDASRLITGIKEFDLVLGGGIINGMVILIGGEPGIGKSTLML
                     QLSEWIGKQSKKVLYCSGEESAEQIRLRSKRLQVINENIYLLCTNDTEHIVEAIEENK
                     PDLVIIDSIQSVSISALDAFPGSITQLRESTNRLLRTAKTLGIPLFLIGHVTKEGVVA
                     GPKILEHMVDTVLYFEGEMRSQYKILRAVKNRFGSTNEIGLFEMTHLGLAEVNDPNQI
                     FLSHEKNDIGTAISCITEGSRSFIVEIQTLVNTANYGTPQRVAVGLDQKKMAMLLAIL
                     EKNLALYMRSSDVFINLTGGIRSTDPALDLAILAALISSMKDKPITEKTVFIGEVGLN
                     GEVRPVSQLDKRISETIKLGYNKIFISGYAKAKANPKVIKVKDIKALFSALQ"
     gene            complement(22808..24940)
                     /locus_tag="PLB65_03415"
     CDS             complement(22808..24940)
                     /locus_tag="PLB65_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ77606.1"
                     /translation="MNRKLIAEFKYDLAPVIRGYNNRSLYINLSTNEIKQKPVTDLMI
                     DKFVGGKGFDLYLMWHGVKDDTKWDSPENEICISFGPLCGNTSYPGSGKSIVTTISPL
                     TGIPVDCNVGGHFGPYAKFSGWDAIELQGIANEEIIVYIDGDRGIVQILSAPDEEINS
                     HILAEELVKEFADDENKYQFVSVVSSGKAADNVLITCLNFSFWDKRRKVARLKQAGRG
                     GTGSVFRHKKIKAMVVKYSGLKVDSNNPVDVETIRMRGLKLHREIEAGDDTQNRMHQV
                     GTAHLMEIMNDYDLLPIRNFKYGQAPEAEGLHSREFKQLFTQGMPDGCWFGCSLSCCK
                     GVDNFEPRTGPYKGQKVCVDGPEYETAAGCGSNIGVFNARDVVEINFYCDTYGVDTIS
                     FGTSTAFAMECYENGILNKERTGGLELTWGNADAALELLHQMARGEGFGLIVGQGVRK
                     MKQIFAEKYGADPNFLQDIGMEAKGLEYSQYLSKESLAQQGGYTLALKGPQHDEAWLI
                     FMDMVNNQIPTFKDKAEALHYFPMFRTWFGLQGLCKLPWNDIEPADNAETDEPAKVPE
                     HVQNYVDIYKAITGKPLDKKTLIEQSERVYNFQKVFCLRMGKGRRIDDVPPYRAVGPV
                     TEEEYLSRQERYDKQLKEKIGINPEGKSTQEKMEILRNYREDQYQQLIDAVYDRKGWT
                     KEGVPKIEHLQKIGMDLPEVIEVVKRFI"
     gene            complement(24957..25340)
                     /locus_tag="PLB65_03420"
     CDS             complement(24957..25340)
                     /locus_tag="PLB65_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOQ77607.1"
                     /translation="MKVLRTYPDKCITCHNCEAACSKLYFKEDNPEKSCIVINEELYP
                     PEMTVCNQCGICAGVCPTLALTINAQEVVILNKSLCIGCLMCVAACPNNSMRYAKGVY
                     NPFKCIACGICTKSCPAEAIEIETA"
     gene            complement(25405..26670)
                     /locus_tag="PLB65_03425"
     CDS             complement(25405..26670)
                     /locus_tag="PLB65_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77608.1"
                     /translation="MNRKVMILFLAILCIMAFTLQGQEIPSSSTFHNGNLKEDNFGLI
                     ALRQNSYSLIKLNQYATNLQDYQAEMKYKEYSSMPEWSKCVTLHSAHEYLCYDNTDYD
                     VSWQQFNSFNTLGVKSKFAGVEVCYNIMNFELDDDHSAQSYNLNKYGIGFGFPNSESL
                     RLFGWLMQSDYRDLIPNLSFTSYKAELAKRVISDSQITELGVEYEYYEVPDIYNAQFS
                     LYDSEYFPQYYRFSNPNTKLSAYLYASSLQNFYLEPCFLNGHSPYKSPLLLANRTAQS
                     LLATFVMEDWDKDNGDLQNINFNAVLSFNFGKYLGLDGQYIYIVNKDNIVEETSKGAG
                     VIADMRLSFISYNIFRIIGNLEYSYYDIDYLDVSENLNYGFYGILQLTNHLNGTIFYK
                     INNVWNRAEDLYETDHSYDILGFTASIRL"
     gene            complement(26866..27432)
                     /locus_tag="PLB65_03430"
     CDS             complement(26866..27432)
                     /locus_tag="PLB65_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOQ77609.1"
                     /translation="MKKLMLLTLCLLLFCSVLLAQADTPKDKAAPKAKTEELKKANPE
                     RPMQKCLEDLKLTDAQKKKWEELKTSFEKTKNTLQAEIKNLKIDLRTALKNENFQNAK
                     ELNKQIAAKRNALDDAKVDFLAARMKELTSEQKATLKKHLEHCPNCHNAPMQNKKAMQ
                     MRHSEKENCNNCGECGSADMPTKINIRK"
     gene            complement(27429..27881)
                     /locus_tag="PLB65_03435"
     CDS             complement(27429..27881)
                     /locus_tag="PLB65_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77610.1"
                     /translation="MSKRWLIAILLISVSFNLAFIGSIIYLHYIHPFPKPPMENEHRQ
                     ASRPPFGPPPFERDEEIRNLRKNFEDIKHSLMLELANDPVDMKKVNALIDSSLIAQNI
                     LEQKLAERMVAYRKTLSAEEAKEHFLRRAEFVKNHSNRQRKSQNRRTQ"
     gene            complement(27874..28320)
                     /locus_tag="PLB65_03440"
     CDS             complement(27874..28320)
                     /locus_tag="PLB65_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HOQ77611.1"
                     /translation="MRCSKAKRYLEKYLDGELDPHLQAKLNAHLDVCAQCKSWLVDAK
                     KMQLMLTALPMADFPSWVHNQIMDKVHRLDSQNPGFFRRYKLAPVTAMLTIALSLLIG
                     IKVGTVSYKNIVPENQDYTISTSVAYGGFGENTILDYVSENGEQDE"
     gene            complement(28366..28854)
                     /locus_tag="PLB65_03445"
     CDS             complement(28366..28854)
                     /locus_tag="PLB65_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HOQ77612.1"
                     /translation="MKKEHFEAFLTANEKRIYHYVLSLCSNEQDTLDIVQTVFIAVYQ
                     NLERIEEETALAYTYKIAHNKCLSFLKQKSRYVNIEPDKFNSMQDKNLNYKEPDYTVL
                     KNAIASLPPRLAAVIQLQYYEKMSYKEISTTLGISIKAVESLLVRAKRILRKKILQDK
                     EK"
     gene            28969..30225
                     /locus_tag="PLB65_03450"
     CDS             28969..30225
                     /locus_tag="PLB65_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOQ77613.1"
                     /translation="MINKSVVYLSRKYLNGRKSKGISKTHYLCLIGITLGVLALLCVT
                     TVMNGFRFDIRNRITGTFSEIRIVANEGETVKNYSSICKQLQDMKFYASPVIRNELLL
                     KYEQVVLPSLCFGIEAEKHKSVSSLLHKPKNDNREIVQGIVAGNIAPEDFNSSGIALG
                     AGLASQLGVYLGDEIQVLSPMFNRPTAFGMIPRIRYLKVQAIFAAGMPEYDQNYSLIG
                     IDNAAFFSSYQDDEADYIEVKPSPNAPQNHIMQTLKKAFPQYQIEDWSSFDPNLYSAI
                     RFEKFLMFIIMLFMFIIASFNLTGNLLKTISQKKRELGLLKALGLTDKDLQTLFLLQA
                     LFLCTVGIIAGLGFGSILLLIQKYTGIVKLGLGGGEAIILPVKFLFTDYLLIVVVAYL
                     ITCLSVLLPLKRLNKINAVELIKRTV"
     gene            complement(30541..31914)
                     /gene="mnmE"
                     /locus_tag="PLB65_03455"
     CDS             complement(30541..31914)
                     /gene="mnmE"
                     /locus_tag="PLB65_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOQ77614.1"
                     /translation="MSNVGEIICAPITPNGFSAISVIRLSGKGCIELVAKHFSQSRKL
                     LALSSHRLIFGTFYSESGEAIDEVLISVFREPNSYTGEDCLEISCHGNPNLVYNIIQT
                     LLLSCRMAKPGEFTLRAFLNGKMDLSQAEAVNDLIQAQASKAEKAALMQLKGKLSNYL
                     QELLNRITELRIRFELAIDFADQDLPMPDINLMKQELMDIIKTAEELKSTGEQGIKIR
                     EGIKICLTGAPNVGKSSLFNALLQHNRAIVTPHPGTTRDYLEECLSLNGFPIVIYDTA
                     GLREAKDDIEKEGIAKSYELMQRADLILYLVEAKQIANLELELPELIKLSTIPPELQS
                     KTLIVFSKADLMEENSPKISSGIYCSVKKENGLQALTKAILNRLMLSDELLTKPIIIN
                     NRHLAALSKCINSLHQADLSLKENLGFEFIAFELISASNALEEILGIITTDDLLDKIF
                     SEFCIGK"
     gene            complement(31907..33775)
                     /gene="dxs"
                     /locus_tag="PLB65_03460"
     CDS             complement(31907..33775)
                     /gene="dxs"
                     /locus_tag="PLB65_03460"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOQ77615.1"
                     /translation="MILENITDPNQIKSLTSSELKILAKEVRNRIVEVVSKTGGHLAP
                     SLGTVDLTIALLYLFDPLVDRIVWDVGHQTYGWKILTGRNAQFDSLRQYKGLCGFTNR
                     DESPYDAFTTGHSSTSISATLGIACGRDLNNEKGHCIAVIGDGALTGGISFEALNHGG
                     HLQKDKFIVILNDNEMSISKNVGGLQKYMTRMLASKSYNVLKKQIWDLSLTLPSNIRR
                     SFIYGAQKLEESMMNILVPNIIFEDLGYKYVGPIDGHDIDQLLRIIKRVKNYMVGPVL
                     IHIVTQKGKGYAPAEKDAALFHGTGPFELKTGKQLSSGKQSWSDFMGSTLVNLAKKNP
                     KIVAITAAMIAGTGLTKFEETFPDRFFDVGIAEQHSVTLAAGMATKGIKPFVAIYSTF
                     MQRALDQVIHDVALPKLPVVFCIDRAGIVGEDGATHQGAFDLSFLNFIPNLIILTPST
                     AEELSAMLSWAADYQEGPVAIRYPRGAAIHSLEHQEPVQFDPFSVKIHSHEGKIALVA
                     CGDAIFIAEEVHKLLEKNNIQSKLVQLLSVKPLNEKALSDLASTCNYIFTFESNAVIG
                     GMGARITQLLSTYPVKVINFGYPDSFIPHGKISELLDEIGFTPAKLYNKISQILNE"
     gene            complement(33792..35711)
                     /locus_tag="PLB65_03465"
     CDS             complement(33792..35711)
                     /locus_tag="PLB65_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HOQ77616.1"
                     /translation="MFQLSFLNATLLFFAAATILPLLIWLLAKKKPQKIVFPSLRFIK
                     LSKEQEKSRSKLKNILLLIIRMLIILLVVLAVARPMFSSTKLKPSHKHPPTAIAILID
                     TSYSMDYAERGKSSLQYAKDILRKINTKANSDDRLIPVSSDENWNLMHSQIYAGTIPE
                     TLIDQLKITYNPLSLEEMIAYAETKLSESQMPNREIYLVSDLRIPPSTIKTKIPVALI
                     PLPETSSYENLACISANALPQLVDKRNQQLIQFTIANYGNIEYKDVLVKAVVNDIKLA
                     EKFVSVPPKSTITETITTELRSDGWQSGYIEINDECQMMDNRCYFAFPFYSKPRIAVI
                     SKNDNLPAILATVLRVYSSATYQVISPDALSLSDLDQYQLFVVYECGAITPRLNEIFT
                     NLRNHKTGLLFCIGNNLAADLKSYLNNNFGLEIKDKTHKAVSIDNLNAHHYISSLISG
                     KPLKNPLIVDYWQAVNKSAGVLVSAQSFPMAVINGKQSLWLWNIASTENPFFIDPAFP
                     VLAFRTLDYIAGSDFPESEAKIGQILTFDKLKLPTGETISSRRYLANEPGVYIFEPDT
                     PRNYAMAINIDYSDSEDRTNFPKGIKNLGDKWEEKLFFSRLGFDLWKILLAIAFALMI
                     LEIIIIKTEEARAKP"
     gene            complement(35712..36779)
                     /locus_tag="PLB65_03470"
     CDS             complement(35712..36779)
                     /locus_tag="PLB65_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77617.1"
                     /translation="MLKRIFTMSNIQFLILWLCPVAVLFAQQITPLPISSYDYNLPEN
                     SVSPMAIGTGGLNITNAEDPFCAYGNPALMADNKLSSLSISYRLTSKEDISFYEALSF
                     SNTLKDKQFKYFVLNTGPVSISYQPVSSIHISQFTNNGLNSEYYDYQLDKVQLSWGGR
                     DERYPKFAAGINVKYLSGRLVYLQEHKVGNSLIRDGFIDDKVKGVSSDLGFTYKMGDV
                     VYAASFYDLLSTLWWENYDSKIIKRRTALGIGFEGENSHYYTGIQSKISKDPDTTFHF
                     GYGYTMNFGKSGTEYTTEDRNSLDLRIGVYSQDFYGTDNINFTLGGGYYYKNFHFDFS
                     LNNTGMKLADSDYLFSLSLGI"
     gene            complement(36779..>37041)
                     /locus_tag="PLB65_03475"
     CDS             complement(36779..>37041)
                     /locus_tag="PLB65_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOQ77618.1"
                     /translation="YRTSNHLGGAFPSSHIAIAIVLTIAALRFIRPIGYICTVITFFL
                     SIATVFCHYHWFIDAVCGIITGIAGYYLANYTRAYLKKKGFD"
BASE COUNT        11209 a         6934 c         6569 g        12129 t
ORIGIN      
        1 caataactgt tgcaccagct aaagttactg agggcttata tttaagagca agctccgtta
       61 acgctttagc ataaaactca tcccgaaaat gctttaaagt aggatcatca acttctaaaa
      121 cctgatcggc tccaaaagct atcaaatcag aagaaagcga ttcaatttga tgtcccaaaa
      181 ctactgccga aagctcagaa ccaagttcat cagcaagttc ccttcctttg cccaaaagtt
      241 caaaaaccac tggggcaatg atgctatcgc gctgttccgc aaaaacccat acgccgttga
      301 attcactttt atcaattgct tcggcctttg cttttctaag caagattgct tcaaaaggac
      361 aagcagaaac acaagcgccg cataaaacac atttatccgg gtctatattg gcaatctggt
      421 cttcaatttt tatagcatca tacgcacatg cacttaaaca ggaaccacaa ccgacacatt
      481 tgtctattaa cacttctatc ataaacattc tcctattttg cattcatttt ttcataataa
      541 tttctgtcaa gattaaagtg tgaaccgaaa aaagtgggaa aatggagagg tgaaaaagta
      601 gaaaagtgaa accagtggat aattataggt gcaaactaaa atataaaagt gttacgcctt
      661 ttctttattt tatatatttt ctctatgaaa agtcattaaa ataatcccgt tagggatggc
      721 agatattaac aacggatttt gaatccggta agaacgaaat aaacattatc actatgataa
      781 aaagttccgt aggaacgaca gagaattaag gcaggtttta acccgttcaa aaaaaagata
      841 aaaaccaaaa gaacaaaaag acagaaatac aataaaatag ataagacata taacggagaa
      901 agtcattagt cagaaaaaaa atgaagattt tatcagagaa aatcataatt ctgtcgttcc
      961 tacggaactt tttcggataa gcaaaagtat ctaaatgaat gacaatacat aaaaatcaaa
     1021 gaattatacc agaaaagtgg tgaatgttag cagaatagag tttccgctcc ttaaagctta
     1081 aagcagtaaa ggcgaccagc attcctttac tgcgttagca ttggtttttt tcgattaaat
     1141 atccaaatta ttattcaaac aaactttttg tgacattttc aggtattttg atgctctgag
     1201 ttttagcaat agtagcgaac tcattaatag tttttatttg agattcaagc agaataggta
     1261 tgtcagttac tacagcagtg gcatagggca aatctttagc atatttcatt ttagccatac
     1321 ctgtggcttt actagcttca tccacataca ttttagcact ctctaagata actttctccc
     1381 ttacaattga gctagcaaga taagtaaagc cacttaaaaa atagaccttt tgcgattcac
     1441 tcaattgggc tttagcagtt tttaggtctt ccagtttggc tttcaccatt tccgaaattt
     1501 ctaattcact tccttccaga tttccagaaa gtagagattt gccttctgct tctaaattca
     1561 catattcctg acgcattccc aatgcgacag caatcatttc ttcagcaatt ttagcatcgg
     1621 tttttgtttg gtcagaattt atagttatta ttttaatatc tgagacctta ggagttaata
     1681 gagcattttc aaaagattga attgcactgg atggaaattg attatagtag tcctgattat
     1741 ccagttttga gtcatcgaga aaaagttttg attctacttc tttccctttg tcttcttctt
     1801 ttttcttcgc ttcaatgctt acccaagatg taagcattga cattatgcaa agagatacaa
     1861 gtacaaactt aaatgttttc atttttcgct cctttttttt ttattataac tcaattttac
     1921 tcaaggctgg tcaactaaat attgagatat attttttact gtttcaaata aagtattggt
     1981 tttagcaact gtcggattag attccaaacc cctaaatcga taaggtactt aaccacagat
     2041 attcatttaa acttcttctc cactgctaaa atttaactgt ttatctttat ccaaagcaaa
     2101 aggggttatt agaataggaa gttctttgtt tatagaagta ttactagtaa atcgttttat
     2161 aaaatctaaa atcataagta gattagtttt tccagaggca tcagcacaat agatcatagc
     2221 cattctaaga attcttagct ttggtttttc agtggaaact acataataat cctccagggt
     2281 tttgtcactg gtagtacgaa aatccaagct tactttatta cggaggggaa agaagttttg
     2341 aacgctaaag gtgataatca ttttttgact ccttgttatg cactttttct gcatttcata
     2401 gacaatttaa ttagacatgg gagaatgtca agtagaaatt tacagttatt ctgcaaatat
     2461 gatgttttat cggcttgaat tgtatttcta aacttactct ctgctaaaag gattaatgaa
     2521 aagagaaggt tatgctataa agttattgat ttagatttgg cagctgattt gaagaatgtg
     2581 ctttttgaag tgttttagtt tctacagcaa atcgtaattg actattcctt tgggaattta
     2641 tctttactta tctgaattca atttggatat aaatttttct ttgatagttg caagagatta
     2701 tgggacttgc cagaagttga tctatacaaa aggattgaca gcaagggcaa atacaaaaaa
     2761 gtggaaattc atagttaaca taaatataaa taataagata gacattcaaa gaggagaaga
     2821 aaatgaaaag taatggatgt agatatggaa cgcatagggt aattgaaccc aaaggtgttt
     2881 taccgcaacc cgcaaaggtt ttaaataacg atatgagcga aatttgggac aacgaaatgc
     2941 tgattgatgt gattcgttta aatattgatt ctgcttcttt tcatcagatt aaaaataagc
     3001 tcattgctaa gggttttaca gatttggaaa aggcatttgc ggaacatgct attgaccttc
     3061 ttaatagaac tggtaaacac aaaaacgaag ataccggttc tggcggaatg ctaattggca
     3121 gagttgctgc cattggggat aagtttgaaa tgaaagaaga agtgaaagtt ggagataaaa
     3181 ttgcctctct ggtttcgctt tcgctaactc ctctcaaaat aaacaaagtg aaaaaagttc
     3241 tgctggataa ggatcaaatg gaaattgaag gtcaggcaat tcttttcagc agtggtattt
     3301 atgccaagct accagatgat ttggatgaaa atttggcact ttctgttttg gatgttgcag
     3361 gcgctccagc tcaagttgag cgtttagtaa agccgggaga tacagttgta attattggtg
     3421 ctaatgggaa gagcggaatt ttatgcaatg ccgtggctaa agagcgggca ggaatttgcg
     3481 gaaaagttat tggggttgtg cgtaaagaaa gctacatacc tacttgtaag acaactggtt
     3541 gtgacgaagt tattctagcc agcgctacgg atgccatcac tattcaaaaa gaggtctcgc
     3601 gtttaactaa tggtaaaatg gcagatgtag ttatcaatgt agtgaatacg gaagatactg
     3661 aattgccaag tattatggca gccagagaac gtggtttggt ctattttttc tctatggcta
     3721 cttcttttac caaagcagct ttaggtgcgg aaggtattgg cgctgatgta gatatgatga
     3781 ttggcaatgg ttatgctaaa catcattctg agattgcact ggatttacta aggcgtaatc
     3841 ctgttttaat gaagattttt aaagaacgct atgcggaata agaaaagagg acaaaatgag
     3901 caagataatt gatcttaaaa acactgccac tccagatgaa tggaatgact ggcattggca
     3961 gataaaaaat cggataacat cttatgaaca attagctaaa tatattgaac ttcaacccga
     4021 ggaagaagcc gttttcaaag ataaagcatt ttcttttcgg atggctatca ctcctcatta
     4081 tctttctttg attgaccaga gcaatcctta tgatccaatt cgtttacagg caattccgcg
     4141 gattgcggaa agccatattt catcttctga tatggcagat cctttaagtg aagatgctga
     4201 tgctccggtt cctggaatga ctcatcgcta tccagatcgg gttttgcttt tgcttacaga
     4261 tcaatgtgct atgtattgca gacattgtac ccgccggaga aaagcaggtg aatatgatgc
     4321 cccaatgccc aaagaaaatg tggataaggc attggactat ataaaagagc ataaggaagt
     4381 gcgcgatgtt attttaagtg gaggcgatcc tctcacttta agtgatgagc gtttagatga
     4441 tatattaaat aaactgagta aaattgagca tattgaaatt gtgcgtttgg gaacaagaac
     4501 tccggtggtt ttaccgcaac gctttactga ttcactttta acgatcctta aaaagtataa
     4561 atttgtgtgg ctgaatacgc attttaatca tcctaatgag cttagtgagg actcctgtaa
     4621 agcacttgcc aaaattgctg aaacagggat tccgatgggt aatcaatccg ttttactaaa
     4681 agggataaat gataatgtgg atgtaatgaa agcattggtg cataaactgg ttaaaaacag
     4741 agttcgtccc tattatattt atcaatgtga tctttctgaa gggatcagtc attttcgaac
     4801 tcccatatcc aaaggtattg aaataataga aagtttgcgt ggccatactt ccggattatg
     4861 cattccaact tatgttgtag atgctcctgg aggcggagga aagattcctg ttatgcccaa
     4921 ttatgtaatc tctcagatgc ctggaagagt tattttgcgg aactatgaag gttttattac
     4981 ttcatacaca gaacccgaat tcgctataca agatgaaagt aaatatcgtg aactttgcgc
     5041 tgatgaatat aaatctaccg aaggaattat gtctttaatg cggggtaaaa aagtagcgat
     5101 cggacctgca gggacaaagc gtcatcagcg tcgtaaccat caataggagc ttttttggag
     5161 ttttcctgga tttccgctaa gcgttgtatt aatatagcga ttgtgggaat ctgcaaaaat
     5221 gctggaaaaa ccactgtctt gaattacatt gtggaaaact atccccataa atggggaatt
     5281 ttaagtacag gacgcgatgg ggagacggaa gatacacttt acaaaactcc caagccgaaa
     5341 gtgaaattac ctgctggttg tcttttttgt gctgatgcaa aaacccttgt tcaacagggc
     5401 tctggcatta gtattttggc taaaacaaaa tggcaaagcg gaggcaggga actatggata
     5461 gccaaaagcg aaattgatct cgaaaccgaa attacaggtc ctgctaatgt aaatggacag
     5521 attgcctgtg ctgaactatt caaaaaatta ggtgctgcga aagttttgat agatggttcg
     5581 ctggaccgta aatctattgt tttaagtgaa gctattgatg gtataatctt agccgcagga
     5641 gccagttttg gttctcaaca ggcaattatt gatgaattgc aacgcttaat aactttatcc
     5701 caaataggaa cttaccatag ttctacttta aaaaagctta ctgagcagaa taagatcctg
     5761 ataaaaagcc agaatagatg gaaaacgaca gctttagtat cgctaattgc caacgaaact
     5821 aaactgctgg aatttataaa cgaaataaac aatcccactc acctttatat tccaggtgcc
     5881 tatacaagta gtgtgaataa tagattgggt aagcatctga aaggtattca acttgttttt
     5941 cgtcatccag aatgtatcaa gcttagcaac atagaacttg aagtgttttt agaagatcat
     6001 aaaccgcttt ctcttattcc ttttaagctg aaagcaatag ctctcaattc acatggagtt
     6061 ggcagtaacg aaatagatgc tgatgaattt catcataatt tacggagcgt ctttaataac
     6121 tatcccttat tggatgtgat ggagatataa atggctgatc gtgattcaat aactcgggta
     6181 actgttaatt tgggtttatt ctccaatata ttattatcga ttatcaaaac ttgtgtaggt
     6241 atttttggcc atagtgctgc tcttttagca gatggaataa attccacctc cgatgttgtt
     6301 tattacattg cagttaagat atttatgaaa caggctcaaa aacctgctga tgtagaacat
     6361 ccttatggcc atcgtcagtt ggaatccatt tctgctattg tagttggtgc ttttatcctt
     6421 accaccggaa ttgccatttt ttgggaatcc attaataagg tctatgacat cctttccaaa
     6481 atagaaactg gacattctgc ttcatcttgg gcattagtta ttgcgatctt taccttttgc
     6541 ctgaaaattt tcctatatac ctatactcgt aaaaatatgc ttaaaactaa taacccaact
     6601 cttaaagcct tggcaaatga tcatctgaat gacataatgg cgtccatagc tgtaattgtt
     6661 ggcgtcttat taggtcggct tggttattat tggatggatc cagctgccgg agcaattgta
     6721 gcaatatata ttattaaaac aggaattgaa attattatgg aatcatccag ggagcttatg
     6781 gattatcttc cagacgaaga ttttagccgt gaaatacgaa ctgaagcatt agctgtggaa
     6841 ggagttcgta gtattgaaga acttggtatc catcgttttg gtccttatta tactgttaat
     6901 atgactatta cggtagatgg agcgataagt gtagatgatg gcaataatat atctgataaa
     6961 gtggaaaaac gcttgctgga taaattcagc agtggattaa ggcaggttca tatacactat
     7021 catccttaca ccgaaaagat ttctctttcc aataatcatc cggaaaataa agaatcacaa
     7081 ggagttaata tatgaattgg ggaaaggata tacttttttc tcttgtcctt tttttgcttg
     7141 catctttttg cttgctcagt atactcggaa tcaattctga accccttcct gaacttaaac
     7201 ttgatatctc taaaccaact gaaccttgga taatagaaat aattcctccc ggaggcagta
     7261 tattttccgt tttagcaaat cagaatgttc ctcctcaaga aattggttta ctggcttata
     7321 actttggtga ttatattgat gtaacgacta ttcaaccggg ggatacgcta aaagtattac
     7381 tatcggaaga taaacagcat ataaagaaat taatgtttgt acaggaacca actactcgtc
     7441 atcattttac tgttagtggc gattctctta tctaccatct tgaggcccta cctgttaaaa
     7501 aagctaaacg gattattgaa ggaactattc aggaaacttt ggatgccact ctgctaacaa
     7561 tgggctttgc tcctcaggat aaacaagcta tcaataatgg cttggaaaca gaaataaatt
     7621 tttcccgcga tgctcgcaat ggcgaccagt ttcttgtttt tgtagaagaa cgcatttttg
     7681 aagggaaaac tctacccggt agaaagatct tctatgttca atatacagga gaaagaactg
     7741 gcacttgtga actcttccgt tatgaagatg cagaagaaaa ttctgtgctc aatggtcttt
     7801 acactataga aggtaaaagc tgtcacagca atggtgtcgg ttttcccctt tctgtaattc
     7861 atgtagtttc ttcttttggt aaacgtcttg atccgtttca tggaagttgg gctaatcatc
     7921 aaggagttga ttatcgagct cattatggaa ctcctgtata tgctgtagca ggtggaaaag
     7981 tgacaactgc aagttataat ggcggttggg gaaatgaaat caggattaac caccccagtg
     8041 gactttcaac tcaatatgcg catctatctt ctatcaatgt tcataaagga caaaatgtta
     8101 aaaaaggaca aattattggt agagttggtt ctacaggacg ttcaactggc gcacatctac
     8161 attttgggct aattaaaggg aagaagtata tcaatccgac aaacttgaaa atggtgggaa
     8221 cagaaaaatt gaatgaaaca cagatggcca gattcaaaag tcagcaacaa acaattctgc
     8281 aagaaatgga gaatttgaaa catcccggac ctctaatcac taaaaaataa aagatagaac
     8341 gcagatagct ggatcaattg gattttgaaa ttagtgcatt gagaatggca atgaactaag
     8401 ttatgagcct tcattactaa ataagtgtga gaacaaggca taataaatta gagcgatgac
     8461 ggcttctgac ttgttaccta tatataattc tctgtgttac tggtagttgc gatgctctcg
     8521 gtataaagaa aaatagaatc cgtaagaact attatcggtg ttttcggtgg cttataaata
     8581 aatcctctta atccttaaat cctgataatt ctgtcctcct aaacctatta tattgcgtaa
     8641 tctgtgaggg agtagtttct tgcggaggaa cggcgttctc cgattaccta tttgtcatct
     8701 tttttttatc tcttaaatct gtgtaatcaa tgttaactat tttcggtgtt ttcggtggct
     8761 tataaataaa tcctcgtaat ccttaaatcc tgataattct gtcctcctaa acctattata
     8821 ttgcgtaatc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtgtaatca
     8941 atgttaacta ttttcggtgt tttcggtggc ttataaataa atcctcttaa tccttaaatc
     9001 ctaaaatcct tgtttctaaa cttatatctt cgtgaactaa tcctaaaatc cttgtttcta
     9061 aacttatgtc ttcgtgaact aatcctaaaa tccttgtttc taaacttata tcttcgtgaa
     9121 ctaatcctaa aatccnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttgtt
     9241 taaacttaga tctgtggaac agccatcttg atattaatct tcgcatcaca cattattttt
     9301 tatcattatt tgattctcca ttcactaact ggctgcgtaa tttaatcttc aatttcagtt
     9361 gttcttcggc ggttagggga ttatttctat ccagttttat gctctcaata aatgttccgg
     9421 gatttatacc catcaagtca attctatcgg agagcataat agcttcgtct atatcgtggg
     9481 taacaaggat aactgttaaa tttaacaaat ggacgatatc tttcagccaa gattgaagtt
     9541 ggctacgtgt tagagcatcc agtttggcta atggctcatc caaaagtaaa atatctgcgc
     9601 tacacatata tgtccttaaa agcgcaacac gttgttttaa tcctcccgaa agctgataag
     9661 gcaaatgttc agtgtaattc tgtaaaccaa atagaggtag cagttcatta attttttgtt
     9721 ttgcttcttg ttcgttgcta cctgttactt tcaccggaag cagaatattt ttcattactg
     9781 ttagccaagg gaaaagtaaa tcgtcctgag gcatataccc caggaaacct gttttagctg
     9841 ttatatcatt gcctttcagt aaaataaatc cctcatccgg tgtagtgatc ccagccaaaa
     9901 gttccagaaa ggtacttttc ccgcaaccac ttggaccgat gatacttaca aattgtcctg
     9961 tccttatgtg aaattgtaca ttatccagaa cagtaatcag tgaaccctta aagaaaaagc
    10021 tcttacggat atcttgagct tccagaatac aatcggacat tttattattt gggtagataa
    10081 tcgttagtaa atgctttatt gggttcaact gcaagctgaa taaggcgttg ctgatacatc
    10141 cagtcacaaa atcttttcca gacaatcggt ttttgccttc cccaggtagg agcatcgctt
    10201 tgaaattctt tagccagata ttgcatacta cgcctaacct gttctgcatt tagctctggg
    10261 acatttttta gcaatatatc cgctgctgaa tctggcttgg aaatacaata ttcataaccc
    10321 ttttttaacg ctgacataaa tttctgggcg gtctcgggat tttttgccaa aaaatcctcg
    10381 ctgctgatga ttacgggtgt ataataatcg aaaacaggat cgatgtcctt taaaggtata
    10441 tagttgagtg caattcctct tctttctgct tccacaccat cccagccata ataaatccac
    10501 tcaaaatcag catccttacc aattgtagaa aagaaatcgt atatcccaga aataacagtt
    10561 acctttttaa aatctgctcc atatttttgc atagttgctt ttaggatagc aagttcggag
    10621 ggcgaatccc aggagccgta ttttttccct tcaaaatctg tcggactctt tattcctgat
    10681 tctttcagcg aggcaaaacc ggaagtatta tgctgaataa cagcagcaat agaaaccagg
    10741 gggatatttt cactacgagc tctgataaca ttttcctgat agcttacacc aaattcgctt
    10801 ttgccggtag ctacaatttg atcggttata ttttgtcccg gttgcaagat atctacctct
    10861 aagctctgct ccttaaaata gccaagttct ttagcaacat aaatacctgt atgattggtg
    10921 ttaggagtcc aatccagagt aatagaaact ttttgcaacc ctttttcatc ctgtcttggg
    10981 gtttgtgctt tacaggaaaa taggcataaa gtaatggtaa ctgctaaaat gagatagcaa
    11041 tatattctta agacattaag tatgattttc atttattctc ctttttcttt cccagagtta
    11101 actttagctc tctcaatatc tttctagaca tataggactc aatgaagaca acttttttag
    11161 cacaaattag ttattgcttt cataagctct tactgccaaa aataatatag gacagtttat
    11221 cagttctttt ggtagtagtt actaataact tacggatatt cactcttcat ttatttcacg
    11281 ctctgggatg gtattgttta tatgtttcca ccattctttt catatctata tgagtatata
    11341 tttgcgttgt atcaatggag gaatgtccta acagtgcctg gacaattctt aaattgactc
    11401 ctccttccag taaatgggta gcaaaactgt gtctgaaagt atgaggagta atatcttttt
    11461 tgatgttcgc ttccaaagct gcctttcgta agattttcca aaaacccatc cggctcattt
    11521 tatcgccgcg attatttaga aatagaactt ccgattgttt caattttaga agaagcggac
    11581 ggctttgttt caagtatttt tcaaatagct catccagcgt atttacataa ggcacatatc
    11641 tttgcttact acctttgcca tgcactaaga taacccgctc cttcaaatta atactgtgca
    11701 aggttaaatc taagagctca gaaatacgta tccctgtagc ataaagcaat tccagcataa
    11761 gtttgttacg aatttccaag ggagtcttta ctggcaaaga atttaaaagc tgaaacattt
    11821 cttctacgct taaaacatct ggtaaatgca ctcccagctt gattcttgga accaaatcca
    11881 tatccacttt ggtggcaata tcgttatcct ttaaaaaacc aaagaattga cctaaagcaa
    11941 ctctttttct ggcaatgctg gaatttaaaa gtccaatatc ctgcaaagaa agaagatatt
    12001 ttgttatatc gcttgtttcg taactgccaa tttctttagg acaaaaaagc aaaaaatcac
    12061 ctatatcacg atggtaactt tcaatgctat tttttgccat tccccgctct accttcaagt
    12121 gataaacaaa actggaaagg tagcttttta acgcaggaga aagctcttct tcaatccccg
    12181 ctgtaatagt tcggggtttc tttagtaatg tggacatcat gaggatggct ttcttttaat
    12241 ccggcagaag tgatttctat aaattctgct cttgcgcgta gatcgtttaa attggctgct
    12301 ccgcaatatc ccattccgga acgcaagcca cctattaatt gatagaggaa atctttcaag
    12361 ggacccttat agggaaccat tccttcaatc ccttcggcaa caagcttatt ttcttcaagc
    12421 tgctcatctt gaaaatagcg gtccttacta cctaatttca ttgccgaaag tgaacccatt
    12481 ccgcgatagc ttttaaatct tctaccatta taaataatgg attcgccagg gctttcatca
    12541 caaccagcaa aaagtgaacc aatcataact gcagaagctc ctccagcaat agctttcaca
    12601 atatctccac taaattttat gcccccatcg gcaataatag gaatgctatt tttatttgct
    12661 tctaaagcgc aatccataat cgcacttaat tggggaactc cgattccggc aattactcta
    12721 gttgtacaaa ttgaacctgg acctatgcca actttaactg catcagctcc attatcaatt
    12781 aaatagcgac acgctttggc cgtagcaaca ttgcctgcta taagttgaca atttaagtgc
    12841 tttttaacat tttgcaaggc aatcccaatg tttttatgat gtccatgagc agtatcaata
    12901 actaataaat ctgctcctgc tttaacaagt tcttgtgctc tttccagata gtcaccctta
    12961 actccaatcg cggcacctac aagtaagcgg tttttatcat cttgtactgc ttccggatag
    13021 tttagccgtt tcataatatc tttaactgtt atcatccctt ccagcttacc atcatcacta
    13081 attagcaaca atttttcaat gcgatgttct tgcaacaggt ctatggcact atccatagaa
    13141 attcccgttt tggcagtgat gagttgttct ttgggagtca tcatttcttt cactttttta
    13201 ttcggattgg tttcaaaacg gaaatcgcgg ttggttaaaa tacctactaa aaaatcattc
    13261 tctacaactg gaaaacctcc aatgtggtga gcttctttca aagctataac atcctctaaa
    13321 gtatcttctg gcgcaagaat ataaggatga gtaactactc cactttcagc tcgttttact
    13381 aaacttacct gtcttgcttg ttcttcaatg gaaaggtttt tgtgaatgat tcctatacct
    13441 ccttctcttg ccatagcaat ggccatagca gattcagtta cagtatccat agctgcactg
    13501 ataacaggta tccgcaaaga aatattggat gtgatgtttg tggttaggtc cacttctgag
    13561 ggcaaaactt ccgattgttg aggaagcagc aaaacatcat caaaagtata tgccttatgt
    13621 aaattcattt tatctcctct ttatttttta tatatttggt ttagaagtag cccagcggct
    13681 tatcttcttt ctaaaatcag gtgagaaaac gctattgggc ttgtttttca aaaattcttt
    13741 agccatccgt tgttcttcat ctctatcggc aactaataat aaacgcaata atgcagcgta
    13801 atctgaagca atttcatctt gaaaatcata ttgaagcaaa gctgctgccc ttgtttcatc
    13861 tccaaaagca atagcccatt caattgccag cagcaatatc tcttcatctt tattatgttt
    13921 aaagatcact tccaaactgt caattccgct tggatgattt aattgcaaca gacgcacagc
    13981 cgaataaaaa gtacttctat cctgctcatc catattcagc gtaaacatca tcagatagat
    14041 ggcatcatta gtatatttgc tttccggata tctgatgata aactcgttca tcaaagtatc
    14101 agccaaagct gtttcaccag ctaaaaaagt atttaaaaag ttcaagtaag atctagtttc
    14161 catcagggta ttataattta tggttttcag tatattatct gctttctcct tttctgaatt
    14221 caaaatatat aatcgtgcta tttccagatc catttcctga gcttccaaat tgttacggct
    14281 aaatttttta gcttcattca gccaaaccaa agcactatct actggttcat ttttagcaag
    14341 cataatttcc gccatcagct tgcgtagttt tacgctaaca ttagagcgat aatacagatt
    14401 tctatccttc cagaaaggca tcgataaaca gccagaaatt atttgctcac ttccaggata
    14461 atctctttgc tgatatttta attcagccat ccgaaaaagc aaatccatct ttttaacctg
    14521 gtctgtttct atattctcca aatagctgta tgcctcaaaa gcaatactgt cattaccaac
    14581 cgctgcttga tcttctgcaa aatgccacat tttttgagga tcaagctgtt tatagatgga
    14641 aagagctgtt ttataatcct tcatatttac caaagcatta gcatagagct cttttaatac
    14701 ggaactttta ctactatctg caatagcgga aacaacagat atcagtgaag gttcttcttg
    14761 caatatagtt ttcagctgat tattagtaaa aaataagtaa accggattac tctctgtata
    14821 agccatatat tcacgaatag ctttttcaaa ttggcgacag ttcaaagccg tatttgccat
    14881 ctccaacctg aataaatcag gtttacccag acggttacga gccattgtat atagctccat
    14941 caccttgtca taaaatgctt tgcgttcaaa aaaggaagct aaccgccgaa atttaaattc
    15001 atcataacca aaatttgcca aatgtcgcat tgcttcctgc caggcatcat ctactttcgc
    15061 ttgtgtaaca agtaattgaa tatagaattc gctataagtt tgcggaggca atattctttg
    15121 gtattttttt aaagttgtct cagctttatc tgtttgcgaa agagtaaaat agaggttcat
    15181 cagttggttt attgaattta agtcgttagg gtatttatcc aggatctgta aaaaaagttg
    15241 ttctgcttga gcatattgac gctgaataag caattgattt gcctgctgag taagaatttg
    15301 cttttcatca tactgtgcat atatagcact ctcgcaagca attagtatta gtaatagcag
    15361 agctatcttt ttcatttttt atttatcatt tgctggtagc ttcattcagc gcttttaaat
    15421 attgcataat aacttgttga tattccttag gataggctcg taaaccttcg tccagaagtc
    15481 cactgcgtct taatgcttct aaatctattt tagtattgcc taagatgggt atgtcttgtc
    15541 cagcctcagc ttttctcttt tctgtaaatt cgcgtttatt aatggaacgc tgggcatcga
    15601 gcattttact aataattctc tcctgtcttt ccagtaattc aggattcaac tgattgtttt
    15661 tcaattgacg agtgatcgcc tccatttctt ctgtaatttg ttttatcaca tttccctgtt
    15721 tttgtgcttc aggattattt tgtaaagctc ttttcaggtt ttcagcaagg cgttcttgat
    15781 cagcagccaa tctttgaatt tgttgttgca tagcactatc catttttccg ccttgagcct
    15841 gcaattgtaa cattaattgt tcagtaagca tattcatagc catttgttct tgactcattt
    15901 gttccagcat ttgtaaaagg gattgcattc ctcctccact tccgctacca gaagaggcat
    15961 tttgcaatgc ctgcatcaga tcataaacca ttaaattaat ccctttttga ataaaagtaa
    16021 ggttattggg aatttgatag aactgcgatt catttatcga gctgaagaca ttgcgataag
    16081 cattattagt atcatttaaa tctatgaaga attttggtgg gatcatcatc agcacttgag
    16141 tttcgctaaa caatttattc agagatatct gaattccttc cgattgagaa atgaggtcat
    16201 gaataatagc ataaggatca tttcgatagc aggcagtggt tgcttcatgc tgtttagaaa
    16261 aaattaaaag ttctctaatc gcggtttgga ctgcctgcag cattctttgt tgatttcctc
    16321 cgcccattga ttctttcatt ttatttagtt ttaaggaaaa ccgtcgcatc ttttccagag
    16381 cgttattttg agctgattga gattcacttc gctgattttg attgagggca ttttggctat
    16441 tttgaaagtc ctgtttcagg ttactgtttt tcatatcttg catcagatcg ttaagttgct
    16501 taataacatc gttatcacgg ggtgattcca acatttcact aatcttgcta agttcttgtt
    16561 gcaatttatc gtaggtgtcg cttatttgtt tttgatcggc agcaaggtct ttggggtttt
    16621 gcttaccgtc catagtcctt tcgcttagtg tcttttgcat tttttccatt tcttcggaaa
    16681 tctgcaaggc tttttgcaag gcttgttctt tttttatact ttccagcagg gcaagggttt
    16741 ggtctatttt cttggcaaaa tcctccatag agaatttgaa attttccagt gcttttttca
    16801 gatcttccgt ttttacattt tgcagcgcat tttcaaattt acgcattgct tcttgcagtt
    16861 cttcattatt gatttcctgc atcaattcct ggattttttg cattttttgc agagtttcag
    16921 gagagagggt ttcatttgcc tgcattttat taatcagttc ctgatagtta tcagcaatgt
    16981 tctgaacctg ttcagagagg ttctcctgtt tttgtaagat ctgttccaac tgttttttat
    17041 cttcccattg cagattttct tttttcagaa gttcgcggcg tttgttctca aattcttttt
    17101 gcaggtcttt cgatttttcc aaagcggttt gcagttcgct tttcttttcg tgttctttgg
    17161 tttcaatttc tctataaatc tcttcaatgg aaggaaatcg cgctttaaat cttgtacttt
    17221 ctgccttatg ttgttctgga gaattatcat atatagtagc ccaataagta acaacatcgc
    17281 caggaaataa attcgcttcc ttcaaatcca aaagaaaatc ggttgtaaac attttgccag
    17341 aaataacact ttgcacattc agcacctgtg gctcagaagc cataatttga atattgagag
    17401 cacagttctt caaaccataa tcgtcatctg cttcaataat taaaggcagc aacatatttt
    17461 gattcagatt aacatcctca ccgggaaaag ttattcttat ttctggagga ttatcttcca
    17521 gaatagatat ggtcttttct tcaggtttgt tttgccttcc caaagcatct gttaattcca
    17581 aataccaggt gcgaggtttt tctatagaaa gttgaacaat gtaattactt ttatctaatg
    17641 cttgcaaagg aagccggctg gcatcatcaa aaaccataac tgcagattca acaggaatat
    17701 ttgtgctaaa agaaagtttc actttactat atttcaaagc ctctatatta ccgtaactta
    17761 aggtatcagt ccaggatgtt aatccggaat atgcaggcgg attatattgc acaaaccact
    17821 ttttcacaat cggttcatcc aaaacctgaa ttttatatat tggcgatttg catacctcat
    17881 tttgcgcata gtattcaatg ctgttatcaa gacgctgaaa tatataacta ttatcagcta
    17941 aacctaattc ccgccattgt ttatctatcc gataaaatag atggtgttgc aaacggggat
    18001 caggattgag aatttttatc tctattggct ggtttctgcc aattgttttg tttccagggg
    18061 ataattctat atagtccttg taaataactt cctggccctt attcgtgtaa aattgcttga
    18121 aggcaagacg aaaatctgaa aacgaatttg cccaaatact gcctaaaccc acaattagaa
    18181 aaagaatagc aaaaactatc caagaaggat agagcgcagg aattttatat tgattctctt
    18241 ttatgcggtg ctctgctaaa atgctcaaat tttccataat cggactatct ttttcttttt
    18301 cagctagttc cagggtattt tgaaagaggt catccctata atcaatctgt ttatccagaa
    18361 acctcgctac ggaaagattg tgccaaaaat tgcggtagcc actccaaagg aaatatatga
    18421 taaccattat taaacaagtc cgtatgccaa tattagcata caagagagcc atagattgag
    18481 aaggcaaatt taaccataaa agaaaatata tgtgcaaagc taccgcaaag ctaagcaggg
    18541 aaccaataat tgcccgcaga acaaagcgta aattaatctt ttgcctatat ttctgaatga
    18601 attttgtaag ctcgctcatt ttatagcatc tcccctttct tgaaagatct ttttcctcta
    18661 ataccgctat tatgtcaaga agtttcctga tgagcacata ttatgattgc aacgagcaaa
    18721 gaagaaggct gatagacaat atgatattta ccagcgcatt ttttgcacaa tatgctaatt
    18781 atcttatagc cctgcctaat gaactatggt acagatgggg tgggaatctt atgggatttt
    18841 ccatagttat gccacgccag tggcaccata actaactatg caatgaacaa gactaaaagg
    18901 gagatattaa atcccgaact tttagagttt ttctctattc tgtaatattt tttttgttgt
    18961 tatgaatgcc accgcaagcg cttgtttaat atactaaaca ataaacacca taaaccctgc
    19021 ttctatatag aatgcagata taatacacta tgttattttg ggtaaaccta agatcatatc
    19081 ctatgcgttg gtttttaaac tagcggtgct ttacaaagtg gaaagtgata atgatctttg
    19141 gcaaactgag gcaatattca gttttccctc atttgatatc attttgttta gttaacaaat
    19201 cttgcaaatg attgatatag ctttctgctc ccccgggtct ataacctgtt ctggcaattt
    19261 ccttcccttc tgcattaacc aaaacaattg tgggaaagcc ttcaatatta aattcattca
    19321 ttttcttttt gtttgcagct ttcattgcat cgctttgtgg tatcccatga ggaaaatcca
    19381 attttaataa taccaaattc tttttggcat aatttataaa ttcttcttta ctaaagacct
    19441 cgctatccag tttcttgcac cagatacacc aatccgaacc ggtaaaatca actaaaatcg
    19501 gaagcttttg tttttttgca actgctattg cttggtccca atcggttagc cattctccgg
    19561 gttttacttc aatttctact tcctgagaag catatttatc aggattattc aaaacatctt
    19621 cgctgctaac aggttgatca gttggggtac aacccaaaac agtaaaagct agcgttagaa
    19681 acaaaattag catccatctc ataattcaat ctcctgattg ttgatggtta ttttggctat
    19741 tacatttgca gcttttttca gcattgcata tacgccacaa tatctttcga gggataaatt
    19801 aaccgctttc cttattttat cgggcggaag ctctttaccc tgaaaattaa aaaaaagtgt
    19861 aattgtttga tatgtaactg gatgctcaga cgatgttttt gcctgagcat ccattgaaaa
    19921 aatatagtcc ttaaccttca ttttctccaa tagggaaaca atatccattc ctgcacaacc
    19981 agtaagagct gcgagtaata aaggtttcgg cctgggccct tcattacttc ctccgaattc
    20041 aggatcggca tccataacta tctcgtgtcc atcaacacaa gcggtaaatt tcatttcccc
    20101 agaccaatgg gtttggacaa ttgtagtcat ttatatccct tcgagagttt ctttcaaaac
    20161 ctcttcatac ttatccacat actgttttaa cgctttataa acagtatcaa catctttttc
    20221 gggaagtttt tccaggattt tttcctgctg atctaaaact gtctgtaaac tattagcagc
    20281 caatttttta cccttatcag taatttgggc ataccagcaa cgacgatctt cttctgaagg
    20341 tctacgaata accaattgtt tatttacaag attatccata attctggtaa cccgactatg
    20401 ggatacatta agcacttttg ccagttcatt catatttgca ggtccattgg ttttgtatag
    20461 atagttcaag agcatacatt ccattcgccc tgcttgtaga catgcttttt gagcatagtc
    20521 aatttcgctt aacaccattt gtaagcgagt aattagatcg tggaatttgt tagcataatc
    20581 agccatttga ttctcctttt tagtgtttgt ttgatctcac tgtttttttt cgatccatct
    20641 ccagatcgaa attaaccgtt ggaaagtaat ttatccaagt tttatgggtt gctaatgctt
    20701 cccttttgcg gatcataaat ttgcgagccg caagaccatt aactacatct atcctagtaa
    20761 atatgttaat taaaaccatc cggaaactcc cttcctgttt tacttaaaat ataaataaaa
    20821 ctactgccta ccttcacttt cgcattcatt tttctatcct ctgttcatat aataacgcaa
    20881 tttttctaaa gagcaaaaac ttttttcatt ttttgaaaat tttctcttgt gtcagtgatt
    20941 gatattttaa ttgcggaatt gctgttaatg atatttaaca aaggtaagca gtaaatttgc
    21001 ttgccataaa atacacatca gattattagg aaataaatgt aaaggagaac taaatggcta
    21061 ctatattttt ctgtacagat tgtggttacg aaactatcaa gtggagcggg aaatgtcctg
    21121 tctgtggagc ttggggaaca atgaaagaat ccactcgtat tgcaggaaaa aataataagc
    21181 tcggagaaac aatcactcag cctaaaccgg aaagaattat agacttgaaa tatgatgatg
    21241 cttctcgttt aataaccggc ataaaagaat tcgatttagt tctcggtggt ggaattatta
    21301 atggaatggt gattttaatt gggggtgaac caggaatagg taaatccact ttaatgttac
    21361 aactttcgga atggattgga aaacaaagca aaaaagtgct ctattgctca ggagaagaaa
    21421 gtgcagaaca aattcggttg cgtagtaaac gactgcaagt tataaatgaa aatatttatt
    21481 tactctgcac caacgataca gaacatattg tggaagcaat agaagaaaat aagcctgatc
    21541 tggtaattat agattccatt caatcggtaa gtatttctgc tcttgacgct tttccaggta
    21601 gtattactca gcttagagaa agcacaaatc gtttattaag aaccgctaag accttaggca
    21661 tccccctttt cctaattggg catgtaacaa aagaaggtgt ggttgcagga cctaagattc
    21721 tggaacatat ggtagatacc gtactctatt ttgaaggaga aatgcgtagc caatataaaa
    21781 ttctacgagc agttaaaaac cgttttggat ctacaaatga aattggccta tttgaaatga
    21841 ctcaccttgg gttagctgaa gttaacgacc ccaaccagat ctttcttagc cacgagaaaa
    21901 atgatatcgg aactgccata agttgcatca cagaaggaag ccgtagtttt attgtcgaaa
    21961 tccaaaccct ggttaatact gctaattatg gaacccctca aagagttgca gtggggttgg
    22021 atcagaaaaa aatggctatg ctgctggcta ttttggaaaa aaatcttgct ctctatatgc
    22081 ggagtagcga tgtcttcata aatttgacag gaggaattag aagcacagat ccagcactcg
    22141 atttagctat tttggctgct ctgatctcca gtatgaaaga taaacccatt acagaaaaaa
    22201 ctgtgtttat tggtgaagtt ggactaaatg gagaagttcg tcctgtttct caacttgata
    22261 aacgaataag cgaaaccatc aaacttggct ataacaaaat attcatttcc ggttatgcaa
    22321 aagcgaaagc taatccaaaa gttataaaag taaaagatat caaggcacta ttttctgcat
    22381 tacagtaaac aatcacggtg atgttatctc tcgcagattt cgctgattct gtagatttta
    22441 tctatattgt atgttgaaag tacgaggaag tgtctcacta ctataaagct cctttgaaac
    22501 gattttggat atacaataat gtatcttcag tgaaggctcg cttcatagat tgacatatga
    22561 actgttttac aactgtattt ttcgttttgg gacagccccg acagatatta ataatgggtt
    22621 ttaacccagc gttaaaatct atcgttacta gcggaactct tatatgaaat cctcttaatc
    22681 taaaaactta tgtttctaaa attcaatcct taaactcggt gagcagcata aaaaaaccct
    22741 cactggcaca aaggagtttc taaaccatag aaaagacagc agtgagggta tagcaatgta
    22801 tattatttta aataaagcgt ttaactactt caataacttc tggcagatcc attccgattt
    22861 tttgtaaatg ttcaattttg ggaacacctt ctttggtcca gccctttcga tcgtaaaccg
    22921 catcaataag ttgctgatat tgatcttcgc ggtaatttcg caatatctcc attttttctt
    22981 gggtgctttt tccttctggg ttgattccaa ttttctcctt cagttgttta tcgtaacgtt
    23041 cttgacggga aagatattct tcttcagtaa cagggccaac cgctctatag ggtggcacat
    23101 catcaattct tcttcccttg cccattcgca aacaaaagac cttctggaaa ttatagacgc
    23161 gttctgattg ttcaataagg gtctttttat ccagtggttt accggtaatg gctttataga
    23221 tatccacata attttgaaca tgttctggaa cttttgctgg ttcatcggtt tcagcattat
    23281 ctgcgggttc aatatcgttc caaggaagct tacataatcc ttgtaaacca aaccaggttc
    23341 taaacattgg aaaataatgc aatgcctcag ctttgtcttt gaaggtagga atctggttat
    23401 ttaccatatc cataaaaatt agccaggctt catcatgttg aggtcctttt aaagcaagag
    23461 tataaccacc ttgttgagca agtgattctt tactaagata ttgtgaatat tccaagccct
    23521 tggcttccat tccgatatcc tgtaaaaagt ttggatcggc tccatacttt tccgcgaaaa
    23581 tttgtttcat tttccgcaca ccctgtccaa caattaatcc aaaaccttct cctcttgcca
    23641 tttgatgcaa aagttctaaa gccgcatctg catttcccca agttaattca agtccgccag
    23701 tgcgttcttt attcaaaatg ccattttcat aacattccat agcaaaggca gtgctggttc
    23761 cgaaagaaat agtatccact ccataggtat cgcagtaaaa attaatttct actacatccc
    23821 gggcattaaa aacgccaata ttacttccac aaccggcagc tgtttcatat tcaggaccat
    23881 ccacacatac tttttgccct ttatatggtc cggttcttgg ctcaaaatta tctacccctt
    23941 tacaacaaga tagagaacag ccaaaccaac atccatcggg cataccttga gtgaaaagtt
    24001 gtttaaattc cctggagtgt aaaccttccg cttcaggggc ttgtccatat ttaaaattcc
    24061 ggatcggtaa caggtcataa tcattcataa tttccataag atgggcagtt cctacttggt
    24121 gcattctatt ttgagtatcg tctcccgctt caatttcacg gtgtaatttt aaacctctca
    24181 ttctgatggt ttcaacatcg acagggttat ttgaatccac tttcaaaccg gaatatttta
    24241 caaccatcgc ttttattttt ttatgtctga acacactacc cgtaccacct ctaccggctt
    24301 gttttaagcg agcaactttt ctccgtttat cccaaaagga gaaatttaga caggtaatta
    24361 aaacattatc ggcagctttc ccgctggaaa ctacagatac aaattgatat ttattttcat
    24421 catctgcaaa ttcttttacc agttcttccg ctaaaatatg actatttatc tcttcatcgg
    24481 gagcagaaag aatttgcaca attcctctgt cgccatcgat atagacaata atttcttcgt
    24541 tggctatgcc ttgaagttca attgcatccc aaccagaaaa tttagcataa ggaccaaaat
    24601 gaccacctac attacagtca accggaattc ccgttaaggg tgaaatagtg gttacaatag
    24661 attttccgct acctggataa cttgtatttc cacaaagcgg accgaaagaa atacagattt
    24721 cgttttcggg gctatcccat ttggtatcat ctttaacgcc atgccacata agataaagat
    24781 caaacccctt tcctcctaca aatttatcaa tcatcagatc agttacgggt ttctgtttta
    24841 tctcattagt gctcaaattt atatacaacg agcggttatt ataaccccga attacaggtg
    24901 ccaggtcata tttgaattca gcaattaact tacggttcat ttttctactc ctcttcttaa
    24961 gcagtttcaa tttctattgc ttctgcgggg caagattttg tacatatgcc acaagcaata
    25021 catttaaagg gattataaac acctttagca taacgcattg aattatttgg acaagcagct
    25081 acgcacatca aacaaccaat acaaagagat ttatttaaaa taacaacttc ctgagcatta
    25141 attgttaatg ccaaagtggg acaaacacct gcacatatac cacattgatt gcaaactgtc
    25201 atttctggag gataaagctc ttcattaata actatgcacg atttttcagg attatcctct
    25261 ttaaagtata gcttggaaca agcagcttcg caattatgac aggttatgca tttatctgga
    25321 taggttctta acactttcat atcaaactca tcccccttta ttgttactta gattcttttt
    25381 aataaaaacg gtgttttccg tcaactacaa tctgattgaa gcagtaaaac ctaagatatc
    25441 ataggaatga tctgtctcat atagatcttc agctctattc catacattat taatcttata
    25501 aaaaatagtc ccgtttaaat gattggtcag ctgcaaaata ccataaaatc cataatttaa
    25561 attttcagat acatctaaat aatcgatatc ataatacgaa tattccaaat taccaataat
    25621 ccgaaatatg ttataactga taaaagataa tcgcatatca gcaatcacac ctgcgccttt
    25681 actcgtttcc tcaacaatat tgtctttatt tacaatatat atatattgac catcaagtcc
    25741 caagtatttt ccgaaattaa aagaaaggac agcattaaaa ttgatattct ggagatcacc
    25801 attgtcctta tcccaatcct ccattacaaa ggtcgctaat aaactttggg ctgtccgatt
    25861 agctaatagg agggggcttt tatagggaga atgcccattc aaaaaacaag gttcgagata
    25921 gaaattttgc aagctggaag cataaagata agcacttaac tttgtattag gattgctaaa
    25981 tcgataatat tggggaaaat actccgaatc atataagcta aattgagcgt tatatatatc
    26041 aggaacttcg taatattcat attctacacc aagttccgta atctgagaat cactgataac
    26101 gcgttttgct aattctgctt tatagcttgt aaaactcaga tttggaataa gatctctata
    26161 atctgattgc atcaaccagc caaatagccg taatgattct gaattgggaa aaccaaaacc
    26221 tatcccatat ttatttaaat tataactttg tgcgctgtga tcatcgtcaa gttcaaagtt
    26281 cattatattg tagcaaacct ccacaccggc aaatttagat ttcactccca gagtattgaa
    26341 agaattaaat tgttgccaag atacatcgta atctgtgtta tcatagcata gatactcatg
    26401 agcagaatga agtgtaacac atttactcca ctccggcata ctggaatact ctttatattt
    26461 catctctgct tgataatctt gaagatttgt agcatactga tttagtttaa ttagagaata
    26521 gctattctgg cgtaatgcaa ttaagccaaa attatcttct tttagattgc cattgtggaa
    26581 agtagagctc gatggaatct cttgaccctg tagggtaaaa gccataatac aaagaatagc
    26641 taaaaacaga atcatcactt ttctgttcat tttttcctcc tgaaaacaat tttggttata
    26701 aatattataa tggctggctt tgtcaatgat tttttctgca ttatttgcat cattcttgca
    26761 cagattgatt ttagtaaaga ataaatacaa aaaccacggg aggagtagcc cgtggttttt
    26821 tctgtgtgtt gctgaggagg gattattttg gactttctat agtatctatt ttcggatatt
    26881 tatttttgtg ggcatatctg cggagccaca ttctccgcaa ttattacaat tttccttttc
    26941 agaatgtctc atctgcattg cttttttatt ctgcattgga gcattatggc aattgggaca
    27001 gtgttctaag tgttttttca aagtggcttt ctgctcagag gtaagttctt tcattctggc
    27061 agccagaaaa tctactttgg catcatctaa ggcattgcgt tttgcagcaa tttgtttgtt
    27121 caattcctta gcgttttgga aattttcatt tttcagagca gttctcaagt cgattttcag
    27181 atttttgatt tccgcttgca gagtattttt agttttttca aaactcgttt tcagttcttc
    27241 ccatttcttt ttttgagcat cagttagttt tagatcttca aggcatttct gcatcggtct
    27301 ttcgggatta gcttttttca attcttctgt ttttgctttg ggagcagctt tgtctttagg
    27361 ggtatctgcc tgtgctaata acacactgca aaaaagcagt aggcagagag ttagcaacat
    27421 taattttttc attgtgttct cctgttttga gattttcttt gtctgttgga gtgattttta
    27481 acaaattcag cacgccgaag aaagtgttct ttagcttctt ctgcactgag tgttttacga
    27541 taggcaacca ttctttctgc aagtttctgt tcaaggatat tttgagcaat taaggacgaa
    27601 tcgattaggg cattcacttt tttcatatca acaggatcat tagccagttc cagcattaat
    27661 gaatgtttga tgtcttcgaa gttttttcgt aaattcctta tttcttcatc acgctcaaat
    27721 ggaggaggac caaaaggtgg acgagatgct tgcctgtgtt cattttccat tggaggttta
    27781 ggaaatggat gaatataatg cagatagata atactaccaa taaaagcaag attgaagctg
    27841 accgatatca gtagaattgc aattaaccaa cgcttactca tcctgctctc cattttctga
    27901 aacataatct aatatggtgt tttctccaaa accgccataa gctacagatg tggatattgt
    27961 atagtcctga ttttccggaa ctatattttt atagctgaca gtgcccactt ttattccaat
    28021 caacaaactt agagcaattg ttaacatagc ggtaacggga gcaagtttat agcgacggaa
    28081 aaatcctggg ttttgtgaat ccagacggtg caccttatcc ataatttggt tatgaaccca
    28141 ggaagggaaa tctgccattg gcaatgcagt taacattagc tgcatttttt tagcatccac
    28201 aagccagctt ttacattgtg cacatacatc aagatgggca ttaagcttcg cttgtaaatg
    28261 cggatcaagt tcgccatcga gatatttttc cagatagcgt ttggctttac tgcatctcat
    28321 ttaactgctc cttattaaaa tttttggttc caattattat acctttcact tttctttatc
    28381 ctgcaatatt ttttttcgta aaatccgctt tgctctcacc aataaagatt caactgcctt
    28441 tatgctgatt cccaaagttg tagatatctc cttataggac atcttttcat aatattgtaa
    28501 ctgaattacg gcagctaacc gaggtggtag agaagcaata gcatttttca gaactgtgta
    28561 atcaggttcc ttatagttta agtttttatc ctgcatagaa ttaaatttat ctggctcgat
    28621 atttacatat cgggattttt gctttagaaa actgaggcat ttattatgcg caattttata
    28681 tgtatatgca agagctgttt cttcctctat gcgttccaaa ttttgataaa cagcaataaa
    28741 cacagtttgg acaatatcta gagtgtcctg ttcattggag catagggata gcacataatg
    28801 atatatacgc ttttcattgg cggttaaaaa tgcctcaaag tgttcttttt tcatagctgt
    28861 ttattctgaa gtgtccttca aattttcttt ttgtcgtgca aaaaataaac caaacctaaa
    28921 acaaagcttg acttattgct atgttttcag ttatttggtc aacttagtat gataaataaa
    28981 agtgttgttt acctgtcaag aaaatatcta aatgggcgta aaagcaaagg catcagcaaa
    29041 acgcattatc tgtgtcttat cggcatcact ttaggcgttt tagctttgct ttgtgttact
    29101 actgtgatga atggttttcg ctttgatatt cgcaatcgta ttacaggtac tttttccgaa
    29161 attcgcatag tggctaatga gggagaaacc gtaaaaaact acagttcaat atgtaagcaa
    29221 ttgcaggata tgaaattcta tgccagcccc gtgattcgta atgagttatt attgaaatat
    29281 gagcaagttg ttttgccatc cctctgtttt ggcatcgagg cagaaaagca taaaagtgtt
    29341 tcttctttgc tccataagcc gaaaaatgac aaccgcgaaa ttgtccaagg catagtagcc
    29401 ggaaatattg ctccagagga ttttaactct tctggaatag ctttaggagc tggcttggct
    29461 tctcaattag gtgtttattt gggggatgaa attcaggttc tttctccaat gttcaataga
    29521 cctacagctt ttggaatgat acctcgtatc cgctatctaa aagtgcaggc tatttttgcc
    29581 gctggaatgc ctgaatatga ccaaaattat agtttgattg gaattgataa tgctgccttt
    29641 ttttcatctt accaagatga tgaagcggat tatattgaag taaaaccctc tcctaacgct
    29701 ccacaaaatc atattatgca aacccttaaa aaagcatttc cccaatacca gatagaagat
    29761 tggagcagtt ttgatcccaa tctatattct gctatccgct tcgaaaaatt cctgatgttt
    29821 ataattatgt tgtttatgtt tatcattgcc agcttcaatc ttacgggaaa tttgctgaaa
    29881 acgatttccc aaaaaaaacg ggaattgggt ctactaaaag cattgggact aacagataaa
    29941 gacctgcaaa ctcttttttt gctgcaagca ctttttttat gtaccgtagg aataatcgca
    30001 ggacttggtt tcggctcgat tctattgctc attcaaaaat atacggggat tgtgaaattg
    30061 ggattgggtg gtggagaagc aataatttta cctgtgaaat ttcttttcac ggattattta
    30121 ctaatcgtag ttgttgccta cctaatcacc tgtttaagcg tgctactccc actaaaaaga
    30181 ttaaataaaa tcaacgctgt agaactcatc aaacgcactg tttagttaat taactgctgc
    30241 gcatatactc ccgaaaagat tcccttatca ttcccatatc gccataaggg atgtatatgg
    30301 gaataatatg atgaacaaca gagaaatgct taatgctatt attggatcat tttttttcgt
    30361 ggggatagat tatttgtgag tgttcaatca cttgggacat agaggtaagc ttttagggct
    30421 atcagctatc caattttttg ctttcttttt tccgattctt gatctattct tttataatga
    30481 ggttataaat acattttgta cctagataga taatgatagt aggagagaaa aaacctgtcg
    30541 ttattttcct atacagaact cgctgaagat cttatctaat agatcgtcgg tagtgataat
    30601 tcctaaaatt tcttccaggg cattgctggc agaaattagt tcaaaagcta tgaattcgaa
    30661 gcccagattt tcttttagtg aaagatctgc ctgatgcaga gaatttatac atttagaaag
    30721 agccgccaga tgtctgttat taattatgat tggttttgtt agcaattcgt cgctgagcat
    30781 caatctgttt aaaattgctt ttgtaagtgc ttgcaaacca ttttcttttt ttacactgca
    30841 ataaattccg ctggatattt taggtgaatt ttcttccatc aaatctgctt tactgaacac
    30901 gataagggtt ttggattgta gttcaggagg tattgtagat agcttaatca attctggaag
    30961 ctcaagttca agatttgcga tttgctttgc ttccactaaa tagagaatca ggtctgctct
    31021 ctgcatcaat tcgtagcttt tggcaattcc ttctttttct atatcatctt ttgcttcgcg
    31081 caatcctgct gtatcataaa taactatagg aaatccattt agggaaagac attcctccaa
    31141 ataatctctt gttgttcctg gatggggggt aacgatagct ctgttatgtt gcagcaaagc
    31201 attaaaaagt gaggatttac caacattggg tgcaccagtt aaacagattt ttatcccttc
    31261 tcgaatttta attccctgtt cacctgtact tttcagttct tctgctgttt taataatatc
    31321 catcagctct tgtttcatta aatttatatc cggcataggc aaatcctgat cggcaaaatc
    31381 gatagccaat tcaaagcgga tacgcagttc tgtgattcgg tttagcaatt cctgtaaata
    31441 attgctcagt ttgcctttaa gttgcataag agcagctttt tcagctttag atgcttgggc
    31501 ttgaataaga tcattaactg cttctgcttg actcaaatcc atttttccat ttaaaaaagc
    31561 gcgcagagta aattcccctg gttttgccat tctacaagag agtagtaagg tttgtataat
    31621 attatataca agattaggat ttccatggca ggatatttca agacagtcct ctcccgtata
    31681 actatttggt tctctaaaaa ccgagatcag aacttcatct attgcttcgc ctgattcgga
    31741 ataaaaagta ccaaaaatga gccgatggga agatagagca agcagttttc ggctttggga
    31801 aaagtgtttg gcaaccagtt caatacagcc ctttccgctt aagcgaatca ccgaaatggc
    31861 tgaaaatccg ttgggagtga taggtgcaca aataatttcg ccaacattac tcatttagga
    31921 tctgcgatat tttattgtag agtttagcag gtgtgaaacc aatttcatcc aatagttcag
    31981 aaattttccc atgcggaata aagctatccg gatagccgaa attgattacc ttaactggat
    32041 aagtagaaag taattgagtt attctggctc ccattcctcc gataacagca ttgctttcga
    32101 aagtaaagat ataattacaa gtagaagcca ggtctgaaag tgctttctcg tttagcggtt
    32161 taacggatag caattgaaca agtttagact ggatattatt cttttccagc agcttatgaa
    32221 cttcttcggc tatgaagatg gcatctccac aggcaactaa ggcaattttc ccttcatgag
    32281 aatggatttt tactgaaaaa ggatcgaatt gtaccggttc ctgatgttcc aacgaatgta
    32341 ttgctgcacc ccgaggatat cttatagcca caggaccttc ttgataatct gcagcccagg
    32401 aaagcatcgc ggaaagttct tctgctgtag aaggagtaag aataattaaa ttggggataa
    32461 aatttaagaa agagaggtca aaagctcctt gatgcgtagc tccatcttca cctacaatac
    32521 ctgctcgatc aatacaaaaa acaacaggta attttggaag agccacatcg tgaataacct
    32581 gatctaaagc tctttgcata aaggtggaat agatggctac aaaaggtttt atcccttttg
    32641 tggccattcc tgctgccaag gttacagaat gttgttcagc aatgcctaca tcgaaaaagc
    32701 gatccgggaa ggtttcttca aatttggtta atccagttcc ggcaatcata gcagctgtaa
    32761 ttgctacaat tttaggattt ttctttgcca aatttaccag agttgagccc ataaaatcgc
    32821 tccaagattg ttttccgctg cttaattgct ttcctgtttt tagttcaaag ggtccggttc
    32881 catgaaagag agctgcatct ttttctgcgg gagcatatcc ctttcctttt tgggttacaa
    32941 tatggataag aacggggcca accatatagt tcttaactct ttttatgatg cgtagaagct
    33001 gatctatatc atgaccatct atggggccta catatttgta gccaagatcc tcaaaaatga
    33061 tattaggcac cagaatattc atcatagatt cttccaattt ctgtgcaccg taaataaaac
    33121 tgcggcggat attggaaggc aaagttaagc tcaaatccca aatttgtttt ttcagtacat
    33181 tataggattt actagcaagc attcgcgtca tatatttttg caaaccacct acattcttag
    33241 agatggacat ttcgttatcg tttaaaataa caatgaactt atccttttgc aggtgtccgc
    33301 catgatttaa agcctcgaaa ctaataccac cagttaaggc accatctcca ataacagcta
    33361 tacaatgccc tttttcatta tttaaatctc tgccacaggc aattcccaaa gtggcagaaa
    33421 tggaagtact gctatgacct gttgtaaaag catcataggg actttcatcc cgattggtaa
    33481 aaccgcatag tcctttatat tgccttaagc tatcaaattg agcattgcgt ccagttaaga
    33541 ttttccagcc gtaggtttga tggcccacat cccaaacaat tctatccaca agcggatcaa
    33601 aaagatatag caaggcaatg gtaagatcga cggttcctaa actgggagct aaatgaccac
    33661 ctgttttaga aacaacttca acgatacgat tgcgaacttc cttagccagg atttttagtt
    33721 cgctggaggt taagcttttt atttggtttg ggtctgtaat attctccaga atcatatcta
    33781 ctccttttgg cttacggttt tgcccttgcc tcctctgttt ttataataat aatttccaaa
    33841 atcatcaaag cgaaagcaat ggcaagtaaa attttccaaa ggtcaaaacc taagcgtgag
    33901 aagaaaagtt tttcttccca tttatcccct aagttcttta ttcccttggg aaaattagtt
    33961 ctgtcctcgg aatcgctata atctatattt atggccattg catagttgcg aggagtgtca
    34021 ggttcaaaaa tatatactcc aggttcgtta gctaaatacc gtcggctgct gatagtttca
    34081 cctgtaggta attttaattt atcaaaggta agtatctgac caatcttcgc ttcactttct
    34141 ggaaaatccg aacccgctat ataatctaag gttcgaaaag ccagaactgg aaaagccgga
    34201 tcgataaaaa atggattttc cgtagaggca atattccata gccaaagtga ctgctttccg
    34261 ttgataacag ccattggaaa ggattgagca gaaactaaaa ctcccgcact tttattaact
    34321 gcctgccaat aatccactat taaaggattt tttagcggtt ttccagaaat aagcgaacta
    34381 atataatgat gtgcattgag gttatctata gatacagctt tatgcgtttt atccttaatt
    34441 tccagcccga aattattatt cagatatgat ttcagatccg cagcaagatt attacctata
    34501 caaaataata atccggtttt atgatttcgc aagtttgtaa aaatttcatt caagcgggga
    34561 gtaatagctc cacattcata aaccacaaag agttgatact gatccagatc actaagtgaa
    34621 agtgcatcag gtgatattac ttgataggtt gcagagctgt aaactcttaa aacggttgca
    34681 agaatagctg gcagattgtc atttttagag attaccgcta tgcggggttt actataaaaa
    34741 ggaaaagcaa aataacagcg gttatccatc atttgacatt catcattaat ttcaatatat
    34801 ccactttgcc agccatcgga acgcaattca gttgttattg tttcagttat tgtacttttg
    34861 ggaggaacgg aaacaaactt ctcagccagc ttgatatcgt ttaccacagc tttaaccaga
    34921 acatctttat attcaatgtt gccataattg gctatggtaa attgaattaa ttgttgattg
    34981 cgcttatcaa caagttgagg taaagcattt gcagaaatgc aagccaagtt ttcatagcta
    35041 cttgtttccg gcaaaggaat aagagcaact ggaattttgg tttttattgt tgatggggga
    35101 attcggagat cgctaactaa ataaatttcc cgattgggca tctgactttc ggaaagctta
    35161 gtttctgcgt aagcaatcat ttcttccaaa gaaagaggat tataagtgat tttcagctgg
    35221 tcaattaatg tttcaggtat agttcccgca taaatttgac tgtgcatcaa gttccaattt
    35281 tcatcggaac ttacaggaat aagacgatcg tctgaattag cttttgtatt aattttccta
    35341 agaatatctt tagcatattg cagagaactt tttccccttt ctgcatagtc catactgtaa
    35401 gaagtatcta tcaaaatagc aattgccgtt ggtggatgtt tatgcgaagg ttttaatttg
    35461 gttgagctaa acatagggcg cgccacagcc aaaactacca gcaaaataat cagcatcctg
    35521 atgatgagta ataggatatt cttcagttta ctgcggcttt tttcttgttc tttactaagc
    35581 ttgataaacc gtaaagaagg aaagacaatt ttttgtggtt tcttcttggc caacaaccat
    35641 ataagcaaag gcaaaatagt cgctgccgca aaaaagagaa gagtagcatt taaaaatgaa
    35701 agctgaaaca attagatacc caagcttaaa gaaaagagat agtcactatc ggcaagtttc
    35761 attcccgtgt tgtttaagga aaaatcgaaa tggaagttct tataataata gccaccacca
    35821 agagtaaaat tgatattatc ggttccataa aaatcctggc tatatacacc tattcgaagg
    35881 tcaagagaat tccgatcttc agtagtatat tctgttccgc ttttaccaaa attcatagta
    35941 tagccataac cgaagtgaaa ggtcgtatcg ggatctttag aaattttact ttgaattcca
    36001 gtgtagtaat gactattctc accttcaaag ccaattccca aagcggtgcg tctttttata
    36061 attttggaat cgtaattttc ccaccagagc gtagaaagca gatcataaaa ggaggcagca
    36121 taaaccacat ctcccatttt ataagtaaaa ccaagatcgc tggatacacc ttttaccttg
    36181 tcatcaataa aaccatctcg aattaagctg ttgcccactt tatgctcttg aagataaact
    36241 aggcgtcccg aaagatattt aacatttatc ccagcagcga atttaggata tctttcatct
    36301 cttccccccc aggataattg aactttatcc aactgataat cataatattc gctattcaaa
    36361 ccattatttg tgaattgact gatatgaatg gaggaaacag gttgatagct aatagaaaca
    36421 ggaccagtat tcagcacgaa atacttgaac tgtttatctt tcagagtatt actgaaactg
    36481 agcgcttcgt aaaaactaat atcttcctta ctggttaaac ggtatgatat tgataatgaa
    36541 ctaagtttat tatctgccat taaagccgga ttaccataag cacagaaagg atcttctgca
    36601 ttagtaatat ttaatcctcc tgttccaata gccattggag aaacactatt ttccggcaga
    36661 ttataatcat aagaacttat aggtaaagga gttatctgtt gtgcgaaaag aacagcaacc
    36721 gggcataacc acagaataag aaactggata ttagacatcg taaagattcg tttaagcatt
    36781 agtcaaaacc ctttttttta agataagcac gagtataatt tgctaagtag tatcctgcga
    36841 tacctgtaat gataccacaa acggcatcaa taaaccagtg ataatggcaa aatacagtag
    36901 caatagaaag aaaaaaagtg attactgtgc aaatataccc aatgggacgg ataaatcgca
    36961 aagctgcaat agttaaaaca attgctatgg caatatgact gctggggaaa gcacctccca
    37021 aatgattgga agtgcgatag a
//
LOCUS       DAOSTH010000013        32525 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_1506_length_32525_cov_11.544102, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000013 DAOSTH010000000
VERSION     DAOSTH010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 32525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32525
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1506_length_32525_cov_11.544102"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="PLB65_03480"
     CDS             complement(<1..1370)
                     /locus_tag="PLB65_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOQ77619.1"
                     /translation="MNKINLEKRWVFEPLNKVVIENRLARLFLQASEEQEVYMEGTIS
                     FSHAQDSFPAEDYIQSDYQDGVLKIILEDIDSDDFRDAIFTVTIPEGVYLNVKTDNNP
                     VNLDNLINKIKVLNENGPIYLQNCQGDMHLENENGLIRLTECEGNIYAKLENGPLSAS
                     KIRGKVLHLENENGPLKVQMASFTEVELYSENGPIFYETIPVENGNFQFKTENGSINL
                     ILPNDFDFTLEATTQWGRVKTTFDLPITFNEDIYTMINGEGTTNIKVSSDNGTIKINS
                     ENRFNLDFVINKLEQIKIALLKVNSEEEKQKVVDMVNKITAYINRLASSIKEESIKDK
                     ITSATAKLKDLVTNFNFRETNDKVINNLEDIGSQIQDALKEGIKNIKESVDDFKKHHF
                     HTESVSAYIKKILDSPQIKPYLGGEHKKKEKEDIADRSRIKILEMLEAGKITAEEAER
                     LLKAIG"
     gene            complement(1367..1726)
                     /locus_tag="PLB65_03485"
     CDS             complement(1367..1726)
                     /locus_tag="PLB65_03485"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 family protein"
                     /protein_id="HOQ77620.1"
                     /translation="MILATCPICKKELIIKELHCPHCEVSFKGNFENSWLAELNASQL
                     EFVRLFLLVQGNLKELQDKLGISYPTVKNRLAEIIGIITKKRPETDKVIDVLSDLEEG
                     FINVDEAINMITQRREK"
     gene            2031..2108
                     /locus_tag="PLB65_03490"
     tRNA            2031..2108
                     /locus_tag="PLB65_03490"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2066..2068,aa:Pro,seq:cgg)
     gene            2158..3915
                     /locus_tag="PLB65_03495"
     CDS             2158..3915
                     /locus_tag="PLB65_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ77621.1"
                     /translation="MSEKKESFTYYLKLLYPFIKKDIGLLLFGLFAMLITSGLRLLHP
                     LILAQIIDHSIPDRNMAQMFQYGGYFVIVVLLSGLLSYLQILLLSRLGIKIITKFKGN
                     VFSHLLKLPVSWFNKQPVGELIARVESDSERVKVLFSDLSITIIGNVLFFIGGFIVLF
                     ILQWQITIYILPAIIICAVGYWFLFKYLNGFFRLIRERYAVISAKITDYVQGIQMLQA
                     LNQEKKALKDVEQASLSKKKVEVHTQMIEYGSQSVFSFMVNVVFIIIVILVSAPKIIA
                     GTLTVGILIVFIQYIYQLVWPLMQISENVMQIQRSFASLKRILELTKLPTEDELYTGT
                     LHPVFEQEIRFENVWFGYQEDEWVLKDVSLTIPKGKKIALVGPSGSGKTTTVSLLCGF
                     YPVQKGRILIDGIPLSEINFREWRRKIGLILQDVYLFPGSILENVRVYNDQIEEAKVQ
                     EAISIVQLNEFIKKLPEGINSELAERGQNISQGEKQLISFARALAFESEIIIMDEATA
                     SIDPQTEAKIQHSMQNLLAGKTALVVAHRLTSVLDADEILYFNEGRITARGKHSELLN
                     NSPEYRKLVELQMLQVQDE"
     gene            3908..5674
                     /locus_tag="PLB65_03500"
     CDS             3908..5674
                     /locus_tag="PLB65_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ77622.1"
                     /translation="MNKHIKWILEQYKAQKWFVLIMVVLTLLSSIVSIAFPYVFKEML
                     DLLKDILQFPDKYPEPMREVYKIIKLLIAIGMAELITGIYPGIRGWVNMRFEHSLRVM
                     YFKHILAKDFSFFQKFKTGDIVTRLTDDLSDYPRVSWFLCSGIFRAFNSFSMILFALV
                     VMFSINIKLTLLSIAPLPIMMLVFYFTADKVYTNYNRNQQAISAINTQLEMSFSGIRI
                     VKSFVSEEKYNRFFDVALAKRFKTEMSMVKLNAILSLIWQYIDYIAQVAVILFGGYLA
                     VKGEITVGTFFLFYTYLSMMIYPLIDLPQLFVTGKQAFVNIDRLEEMKNYPSLNDKFK
                     GEHKVKDFCALSFENVSFQYPDKPFPVLKNASFNLDRGERMLILGPTGSGKTTIANLL
                     LGLLKSDSGTIKINGKPIDEIDIKSLRNIVAYVPQEPLIFGGSIKDNILFAVEKASEK
                     DYHIAVQTSQLENEIASFPERDETFVGNRGLSVSGGQKQRLTIARALVKKPQLLILDD
                     ITASLDAENEEKLWQALDENYPGIAAIVISHRLSTLRYVDSVLFIDSQGNTHKGTHDY
                     LVFNNKEYHDFLREHLKQENKK"
     gene            5735..6832
                     /locus_tag="PLB65_03505"
     CDS             5735..6832
                     /locus_tag="PLB65_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOQ77623.1"
                     /translation="MRRSFLIILLCAILTFNYADELDDKMRQLREMQKQLESTQQKVK
                     QTQSKKQQTENEIKRTSSLKKRTEANLNKLRLDEKIAQDSLKSVSERIVATEMRIADM
                     HKEQNTELDILLRVDRSYKKQNINHRDQRLLKTLIQGTRQKINILSGFKISLIQVQEL
                     HRKQASKLSRGVKEESYKKTTYEKKIRNLSTQSQKLTIEQKKLQAQVEQLKKDSAELE
                     SLIAQLMKQQGKEPASYQFTGKKIVWPLRGKIIRNYGEETRSYGTSVVSNGIDIAAKE
                     GTKVVAADDGEVIFSDRYGGQGKLIIIDHKNGFFTLYAYNSELLVSRGAKVKKGQTIA
                     KSGMTGSASEPSLHFELRKDGRAINPLPYLE"
     gene            6842..7957
                     /locus_tag="PLB65_03510"
     CDS             6842..7957
                     /locus_tag="PLB65_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HOQ77624.1"
                     /translation="MLILKDPGLRKLEKFLDSNSLEHSTILHWTGSMWGIGCRLSSLE
                     TIARIETLKQRAEKQGFIALIPDINYLDQKQIPAALRPLMHQYWPGNLTFIFPYQDSK
                     FETITNKGKVAFRVPSDPALRAFISLLGEPLISTSVNVSGLMPEENYDRIISKYGSWF
                     DFALLPANKEIKSTTEPSTIIEYISAADSGNNDEIKCLREGSIPFYEIKQAFKLPVVM
                     FVCTANICRSPMAEYLFRKMARENNISLATDSCGLLPGGQMISVNALQLLLERGILDA
                     QQHISQQITPQLVSSSWLILTMEERQRDLLRTTYPNSESRIKTLNEIVGEKGDIEDPF
                     GRNLESYRKTCEIIENRLQLLTEYLKNNELNIQKKII"
     gene            7954..10161
                     /gene="purL"
                     /locus_tag="PLB65_03515"
     CDS             7954..10161
                     /gene="purL"
                     /locus_tag="PLB65_03515"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HOQ77625.1"
                     /translation="MKDYEITPKVIAEHNLSDEEYERITNILNREPTIVELGIFSVMW
                     SEHASYKNSVLLLKTLPKEGSSLLAKAGEENAGLIDIGHGLAISFKIESHNHPSALEP
                     YHGAATGVGGILRDIFTMGARPIAALNSLRFGNPGNPKVQHLLKEVVRGIADYGNCFG
                     VPTVGGEVYFDDSYEGNPLVNAMAVGIVKHNKIARSAASGVGNAVLIVGAKTGRDGIH
                     GATFASVDLSEKSEEKRTAVQVGDPFLEKLLLEATLEAIDEELVVAIQDMGAAGITCS
                     TSEMAGKGNVGIEIDVALVPQRETDMTPYEIMLSESQERMLVIVKPENLEQIKSIFTK
                     WELDAVQIGKVTDDKMLRVFYGEEKVAEIPAETLILGGSAPVYKRESKYPEYLDALHN
                     YDIENLPEPDTYQEVLLKLIASSNISSRHQIYQQYDHMVQIGTVVEPGSDAAVVRIPD
                     TDLAIGLSTDCNGRYCYLDPYLGALLAVAESARNVACSGAKPIAITNCLNFGNPYKPE
                     VYYCFKESIRGMSDACKALGTPVTGGNVSFYNENPEGAIYPTPVIGMLGLMTDYRKAT
                     TQYFKHENDIIVLLGSLKASFGGSEYLKIQHNIIAGKPPIVDLEEELHLQDLILELID
                     NGLVNSAHDISEGGLLIAVLESCFNPEQLFGAKLSFDLELNPAKIYFGEAAGRILISA
                     AKDKYNSIKEAAMKHGIEYHKIGIVSANSSFIVNNHINEDMAILHSAWKQGII"
     gene            10183..10701
                     /locus_tag="PLB65_03520"
     CDS             10183..10701
                     /locus_tag="PLB65_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77626.1"
                     /translation="MNFVFSNFFWGLFFILLGLSVFLKAFNIHLPLVRVFIAIIIIMI
                     GIKLLFGLKSHHYHKPKSYSKENSIYYSSSRDEYNIIFSSGIIDLSDLPENAKDFEIT
                     VVFGSGTVILPDNIAVNINPTSVFGSTILPPKNPVTQDKPVEIESTVVFGRLEYKYAK
                     AEQTTEQTTEPQ"
     gene            10655..12883
                     /locus_tag="PLB65_03525"
     CDS             10655..12883
                     /locus_tag="PLB65_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOQ77627.1"
                     /translation="MPKLNKQQNKQLSPNKEHTNIKLTLWKKPIILLLLFFIFNILTA
                     EKLGYALSGGGARGFAHIGVLKVLEEEGIYPDYIAGTSIGAVIGALYAMGYSANDLED
                     MCLTLNWNELISDTQKRKDLYIGQKRWTPYGNVIFDLNESWIPQIPSSVFVANNLNLE
                     LFKLTAASSQINNFSDLPVPFICNATDLITGEGVTFTKGSLMQALRASISIPSLFEPF
                     ELNGHLYIDGGISQNMPINLLHEMGADKVLGIKVNSTLRKEENLNNFVDILDQTINIG
                     ITRNLNEHLDVCDLLIEPDLTNFASTDYQHIQSIIAAGEESARQMIDQIRAFKKELSL
                     SPERIINFDKHLDNFPVANIFVYGNNTISAAKVREYLGLETGHTYTSEQIKKACINAW
                     NSQSFKTIYPVLEKRNDDKYNLLVYVKEREHKHLAVNLVYNNDEQLMAGLVLSLDNYL
                     LKNSRLLGELKLGGRNEFNLDYVKNFGEQWGAYYRLFPYVTEKTIYNYRDHYKTDSVK
                     STEWGINSGVGVFAKDLAIAELFIYSSQTNLHSDISQTPFLPKRSVISGFGVKGYHES
                     LDDYSFPTSGARVFGKFNFSRYGEVSDYLYSCFQGRAEFYVPIMNLFSVYSEFNYGSY
                     LNSSQQTNLDPFVISGIDGFMGYSRYEVSAPHYQIISAGISSLISKRFFLDIGIQSLR
                     YSDQNIFENPEDWEYCGYLGFGYKNSILPVKLYVSLNEDKDLHSFLSVGYDFDIFFFS
                     RR"
     gene            complement(13071..14441)
                     /gene="glmM"
                     /locus_tag="PLB65_03530"
     CDS             complement(13071..14441)
                     /gene="glmM"
                     /locus_tag="PLB65_03530"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOQ77628.1"
                     /translation="MNKLMTSVSGVRGVFADTLNPIIAIKYAAHFALIQKRHYPEEKP
                     HIIVGRDSRTTGQAMLHSIISAIISVGCDVTDLGIVSTPTLLLKVQESDAIGGIAITA
                     SHNPPQWNAMKFVDADGMFLSPEKAEYFLNSVENEIIWAAWDSMGKITKDNSAIQEHI
                     DRILAIPYLNLEQIRSRKFKVVLDSVNGAGGLISPYLLEGLGCTVYEINSEATGIFAH
                     TAEPLNENLHQLEEAVTFYKADIGFATDPDVDRLSLVSEQGKCIGEELSVALAELFVL
                     PKKRGDIVVNLSSSMISDDIAELFGVQVHRTKVGEINVGKKMQAIKSPIGGEGNGGII
                     CPDVHYTRDAIAGMALILGLLAEQDKPLSAIVDSLPKYYFAKDKLILDASRMENVMQN
                     IPSFFEDYQLDTQDGIKAIGNKHFIHIRKSGTEPIIRIYVESESIEKSRALCEETKKI
                     IERASI"
     gene            complement(14445..16229)
                     /locus_tag="PLB65_03535"
     CDS             complement(14445..16229)
                     /locus_tag="PLB65_03535"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ77629.1"
                     /translation="MLDQLRKKQKVIIYIVAFVFIVGMGAIGLGELLSPKPYLGKVNG
                     TKITFDMFQQKTQEMYDRYAQMYKDQPIDENTRKAIENQAWESLVNDILWQQQIKKHK
                     IKITDDEILTEMQNNPPQELMQNEALQTNGRFDRSKYLSALKNNPQFFAAMEEYVRSY
                     LPRQKLQNKIKAQAGITIDSLKAEYAKDSDIVSGKFIWFDPNKSDPVTVTDAEIKAYY
                     DKNKETEFKKGPASRMKYIAFEEKPSDKDYASIKLLADEVYNRVIKGEDFATLAKEYS
                     DDPGSKDNGGSLGEFGKGQMVPEFEKVAFSLKVKEISKPVKTNFGWHIIRCDSIASQD
                     PENYRIKASHILLKVDPSDETKNALLDKVESARALIKKKGIDAAAKELKVEVQKTDWV
                     PHDQEYIPGIGNNGALLQFMRKGKEKAVSDIITDQQNRKIIAQLTDNKKVYYEDFESV
                     KLRIKYQLEKEKKVTNIKKKAEEFVAKYPSGEYFSAAAKEGWQIIDLNNHKKGQYVPN
                     VGLCEEFTNAALALEAGKTSGLIHTKEGSFIISCSERIKPDFNAFAKDKDKQEEIRKR
                     LEDAAWNRWYDQMRKEAKIIDNRAKYGM"
     gene            complement(17699..19261)
                     /locus_tag="PLB65_03540"
     CDS             complement(17699..19261)
                     /locus_tag="PLB65_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03986 family CRISPR-associated RAMP protein"
                     /protein_id="HOQ77630.1"
                     /translation="MEKGKINKRTTKKNIICLELEKNDGKIVTISERYIRNHEVWKTL
                     ADGVYDVEYELMNGKPVKIKIGSTLIDLTQTQQPNKNPNYHQAHSNPDNENSVDTATA
                     PYNFIPLNDVIFTTEPKCIPPFNQYKNNNGYIDLDITALSPIYIRKTLTEEEEKKLVE
                     LENSNNLAELADFKLSLSGFYKPADAEFRIPGSSLRGMIRTLYEVVTYSKMTFIDENE
                     KLMYRGVKEKKLYQHSIGELRPEKHKSKELDMAEAVFGYIKDNDILPGRVYFEDAICD
                     TPDKILKEQNPKILSSPKPTSFQYYIQQYPKIGNLEKNKINRLKNYDSPNACLAGFKF
                     YWHRDSNDWTANPDDVRKNKTQYTKIQPLDKNSHFKGRIRFTNLSDTELGALLFILNL
                     PEGCAHKIGMAKPLGLGSIRIDAKLNLIDRQKRYTNLKDEGIKEIEDFSPYLQAYYQL
                     LNDNLNIQINDPWTDIERLKQLKCMLDFDHKPSSEKTRYLEIEHLTPNGVKENEYKLR
                     RILQKPCQLVNS"
     gene            complement(19261..19824)
                     /gene="csx19"
                     /locus_tag="PLB65_03545"
     CDS             complement(19261..19824)
                     /gene="csx19"
                     /locus_tag="PLB65_03545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Csx19"
                     /protein_id="HOQ77631.1"
                     /translation="MNEVQSINSEITLLNKITSLEDIFNNLKKDTKYQVVVYLLSEIA
                     FGDYYNGFQLLNNNLEYNNIEAIRIFNQDTELYCYRTNDINSTPPSTFLRGRVRTDSK
                     GEHTYYKDVSQILMGKTNGQQVSGYSIIREARGFQLTLPYSWIKNYPDNSRVTLLTRN
                     YLEHWDNGQLSYFDHRFVRIGETDREE"
     gene            complement(19817..21166)
                     /locus_tag="PLB65_03550"
     CDS             complement(19817..21166)
                     /locus_tag="PLB65_03550"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOQ77632.1"
                     /translation="MKTGRIILGGTLNLLSPLMIGSGRDDISDRDVLVDSEGIVFIPA
                     TSFLGVLKTYFNVDIDKDYLGLYLSEPVNQSFLICDDLYPCSSKGNCRIRDGIRIDNS
                     KGIVEDKAKFDYQVVEPGLNFTFRLEFVINNENRYIQAQHILEECLGVFEHSFFLGGK
                     RSSGFGKLKAGNLYVNEYDFDTASGLTQYLLELQSSNNIENYKPKFSTYDTFVIKADF
                     HLKNSLIVRTYPEDVYGPDAVHISSQGKPILPATSVRGVLRAQAERILYTIWQDVDFI
                     EDFVSSMFGTAVTKNKKYTIPSALYVNEVPISNVTTELQNRICIDRFTGGTIEGALFD
                     SMPVFPQDSKTENITHLTLRLEHPLPSQKGLLILLLKDLYTGDLAIGGEKNIGRGTLQ
                     GLKATIFDQNKKNSISQITELPDNFTPYLEALIKNSDKDQIQNNLAIYKDKGGKVNE"
     gene            complement(21153..22634)
                     /locus_tag="PLB65_03555"
     CDS             complement(21153..22634)
                     /locus_tag="PLB65_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77633.1"
                     /translation="MYKALKLKIELKSPLIIAHQSGDSTLTKTLSYIPGTSILGVLAS
                     NYLKKNGDMPEFYRLFIRGGLCFHNLYPEYQSHIYLPCPHNIWQNKNNNKKVLNIFNN
                     EAMDLDYRPIESFAYVNDDDDIMLYQPHKEIFFHHERDYATGIPKQSIVFCYEALSPE
                     QVFVGTIYGDEIDLQLIKGLLENNLSFRLGKSKTAQYGNCLLKECEIQEHNIQNFASS
                     DNPLLVLISDTIIKNDYGCSTIETNQLEKILGVKILHSSIISGRIETVVNAYKAKKSS
                     ELVYKAGSCFLLEKMPENFSNLLLYGLGERCNEGFGQIKFMKNPTNRLKIISTTMNSV
                     QKPATNPPDIIKGILSKSIEQIITAYLTQVANDKAGTIKHYKINNSLLGRLESFAEHG
                     NFNESIIQLKKLAQDKLKQVYLTEDKKNFYDYLMSIDTIVSTFLENTKQLYVSDKKII
                     NDVYAEIELDKFSLDKAKKIFLKALFLCLRRENQRSRRTNENR"
     gene            complement(22619..23290)
                     /locus_tag="PLB65_03560"
     CDS             complement(22619..23290)
                     /locus_tag="PLB65_03560"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAMP superfamily CRISPR-associated protein"
                     /protein_id="HOQ77634.1"
                     /translation="MIYKIILKTLSDTLIGSGKSSGTIIDSDIVFDSHCLPYIPAKRI
                     KGLFRNAAQDLANFKGFKELTELTRIPDLKFGNIGENDPNPKIIFENLELQDKDNIAI
                     WLNYLTEQFPYLANTLIIKEYFTELRAQTAIDKDTHIAKEHSLRTSRVLKRGFTFEGA
                     LILNAPSQEVETYLAAICFYIKRIGTMRNRGFGKVKIDLISLDNNVNLTAKFAESWRE
                     KCIKH"
     gene            complement(23235..24704)
                     /locus_tag="PLB65_03565"
     CDS             complement(23235..24704)
                     /locus_tag="PLB65_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77635.1"
                     /translation="MAYGLFIDLRAIQDYIFASNRLKDNIGASYLIKHLLEEFLTDQG
                     YCGGGNLFLLCKDEKEAKETIYSLSRKILNECPGLSFSAVYEENFNLDDFPAAFSLLQ
                     EKLRKEKEHRIQQTTVPSFGINAQCNYSGYAAVEIDHSREDELLSEIVYAKRIASKKA
                     EEETKQLYAAELSDSYIFPEKFDDLGIDKGNESYLAVVHIDGNGIGKLFAEQKDLDST
                     IKLSNKIDELCLKAFKAIVSKTVEKCNKSKKKENALNDTSSIGGKYLPLRPLFIGGDD
                     ITFVCKGRLGIPLAIEFIKQIKEDSDDLLLSFCAGIAVANYKYPFYQVYQMASSLCRS
                     AKNKRLTNNNNDSYLDFHLINASVYDPLDEIRSKNYILPQEKSLTRRPYSLSELEQLL
                     NATYDMYSKWPNSKIKELRSALYKSEHQLIALLKQITSREDLPIKFTGFEDYSDTLFF
                     NNQTIYLDMIELMEMIPLEENNDLQDNFKNPLRYADWFW"
     gene            complement(24707..25837)
                     /locus_tag="PLB65_03570"
     CDS             complement(24707..25837)
                     /locus_tag="PLB65_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77636.1"
                     /translation="MVEVLILVGTNPVPCYVSAIYLKNHYNNSRISLICSKENKTLGQ
                     TGTEDIGQRIKTQLIKEGKFKEEDFNQIISIDDVTSFNSIECKLNKYINEEDKKAIHL
                     NFTGGTKTMAAFCYYYLKSHYKSFSSSYLDSRRKIMIIDGNVADSQIDLRQDPMLAIN
                     LETMAVLQNQTDIKPEEYKEKYPELLEKLIDLLDNGNLEEIYGKRNNDLEHMANQLNI
                     TPEKNRLLESVNIKYEELNRNQREDLEDFINGRWLEYYVYNELIKLTGNIQFYFDVHN
                     NQYQVDIVAIYGYQLTLISVTTDKTKPLCKLKAFEAITRATQLGGEEAKTILISRTDE
                     PDNLQRDVEKYIGTLVPNFRAFGETQLKDKSIFPAIINYIKE"
     gene            26452..28038
                     /gene="secD"
                     /locus_tag="PLB65_03575"
     CDS             26452..28038
                     /gene="secD"
                     /locus_tag="PLB65_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HOQ77637.1"
                     /translation="MKKFSWRGLSIVIFICVTAFFLAPLAIPNLPSWWSKHHLKLGLD
                     LRGGMQILLEVDTSELSATDARNAVEQNIKIIRERIDQFGVAEPTIQKLGEKRIMVQL
                     PGVSDYKAAENLIKQTAMLEFKLVAPSEEAKRVIDAIDKNISGNLDKFPALAELAKKD
                     KSIIADTTSTDSLQVGTGIFSSLIRPGEMDFEVQYSDVRLIQELISDTLFAQFVPIGY
                     QLALDKADTENPRADRALYVLNSAPQLTGADLAKAKVEYGSATSTDPRVANKPYISIE
                     MKREGARKFERVTADNVGKRLAIVLDGVVYSAPNIQERIAGGRAQITGRFTATEANEL
                     AIVLSTGNLIAPIKPVSTSIIGATLGSDSIRKGTFAGLIGLIIVALFMVIYYKGCGFI
                     ADIALVLNVGFILAMLTAFGATLTLPGIAGIILTIGMAVDSNVLIYERIREELDIGKA
                     PRTAVDAGFKRAIITVWDSNLTTLIAAIVLYYLGTGPIRGFAVTLAIGIMSSMFTAIV
                     MVRAILDNFVLTGTKTKLSV"
     gene            28051..29034
                     /gene="secF"
                     /locus_tag="PLB65_03580"
     CDS             28051..29034
                     /gene="secF"
                     /locus_tag="PLB65_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOQ77638.1"
                     /translation="MRILKNPNIQFVKYRYWAYAFSIVVLLIGLGGIIFNGLNWSIDF
                     TSGIAATLNLKAKDPNVAPLPIDELRSVLAKNGFPDAEIMYVGDEANSVFQIKIKTTG
                     KGSNISAEARSKLIDIIQSEFPEYVKGRDINKEVIEEIYEVGPKVGSELRTQAFWAVL
                     LALIAMIIYIWFRFELTFGLMGILALFHDVLIIVGIFALTGKEITMQIIAALLTIVGY
                     SINDTIVIFDRIREDLKKNRKDPMPVIFNKSINETLSRTIITGGTTFITSACLFFFGG
                     SVIHDFAFAICLGILFGTYSSICVASNLVIDLHQITGKEKKTLQHLTKKKK"
     gene            complement(29449..30426)
                     /locus_tag="PLB65_03585"
     CDS             complement(29449..30426)
                     /locus_tag="PLB65_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HOQ77639.1"
                     /translation="MIPKRIGFTTSFPVEVLFAAGHIPVDLNNVFISQDSSNYVQNAE
                     LKGFPRTLCSWIKGNYIAALSSNLDMIIGIVVGDCSNSNSLLDMFTEVNIPIYRFSFP
                     ANRNPEDLDKEITKLENYFGVSRSDTLKAKKRLDEIRKKLLVLDEWTWKERLVSGLEN
                     HLWQVNSSDFGGNPDRYESELDMFLKEAEKRDPLPTKLRLAYLGVPPIYKNIYDTIID
                     LGGDVIFNEVQRQFAMPYLLPDIVQQYIAYTYPYSVFDRLKDIKPELEKRNIEAVISY
                     TQSFCHLQLDNLLLRKYIDLPFLTLEGDQPEDIDARTLLRLESFFEVHS"
     gene            complement(30416..32326)
                     /locus_tag="PLB65_03590"
     CDS             complement(30416..32326)
                     /locus_tag="PLB65_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOQ77640.1"
                     /translation="MSLIQVINASIEFGGNYILSDINCTLEHNSKIGLIGSNGSGKTT
                     LIKLMLGVLLPSEGKVLHSKKCRIAYLPQNPVLDGNISFINYVHSSRPDILKLQKKIN
                     ELSELLQDNHSEANETELNDALEKYQAIGGDEFENDLKFIITSLGFTEDDYPKSLNLF
                     SGGEQSRICLARILMMSNDLLILDEPTNHLDVAMISWLEKYLNASNIPFLVVSHDRTF
                     LDNTVSVIFHLENKHLSITKGNYSSFEQAKAIELKTQERQYARQQKFIAETEDFISRN
                     IAGQKTTQAKSRLKLLSRMEVIQNPQQQKKIKLNIQSTGRSGNDVYTLKDVELGIFEN
                     APLAKNVNLEVHYRDRICIVGPNGCGKTTLLKILMEEKDIFSGSLKIGASLEIGYYDQ
                     HQVLLDEELTVMDTLWQLVPNETRGYVLSFLARFGFRGDDVDKKVCVLSGGEKSRLML
                     CTLIHQTPNLLILDEPTNHLDIAMNNSLLSALQEYRGTIVFVSHDRYFMKQLATKYWV
                     FHKALENDLLYPTISEVDMGLEQALELAFSIPELPKKAPVLRERKKKINPWHLEQIHK
                     QIEQEQATIEESNIKIENINSELALSSTYSNELRAKELCQQIELLLSNINKSKQRIAE
                     LENKYLELSYDS"
BASE COUNT        10153 a         5636 c         5800 g        10936 t
ORIGIN      
        1 ttcccaatag cttttaaaag ccgttctgct tcttcagcag taatttttcc tgcttccagc
       61 atttctaaaa tttttattct actacggtca gcaatatctt ctttttcttt tttcttgtgc
      121 tcacccccca gataaggttt aatttggggt gaatccagaa tttttttaat ataggcagat
      181 accgattccg tatggaaatg atgctttttg aagtcatcca cgctttcttt gatgtttttg
      241 atgccttctt ttaaagcatc ctgaatttga gaaccgatat cttctaaatt attaatcact
      301 ttgtcgttag tctcgcggaa attaaagtta gttaccaagt cctttaattt ggctgtagca
      361 gaggttattt tatcttttat cgattcttct ttgatcgagc tggccaggcg gttaatatag
      421 gcagtaattt tatttaccat atctacaact ttttgttttt cttcctctga atttactttt
      481 agcaaggcga tttttatctg ttccaatttg tttatcacaa agtccaaatt aaagcgattt
      541 tcggaattga tcttgatggt tccattatca gaacttacct taatgttcgt tgttccttcc
      601 ccgttaatca tcgtatagat atcttcatta aatgttatcg gtaaatcgaa agtggttttt
      661 actctacccc attgagtagt tgcttctaat gtgaaatcga aatcattggg aagaatcaaa
      721 ttaatagagc cattttcggt tttaaattgg aaattgccat tttctacggg tatggtttca
      781 tagaagatag gtccattttc tgaatataat tcaacttcgg taaaagaagc catctgcact
      841 ttcaaaggtc cgttttcatt ttccagatgt agaactttgc ctctaatttt ggatgctgat
      901 aatgggccat tttctagttt ggcataaata ttaccttcac attcagtcaa tctaattaag
      961 ccattttcat tttccaggtg catatcgcct tgacagtttt gtagatagat ggggccattc
     1021 tcatttagaa ctttaatctt gtttattagg ttatctaaat tgacaggatt gttatctgtt
     1081 tttacattca gataaacacc ctcaggaatg gtaacagtaa aaatggcatc tctaaagtca
     1141 tcagaatcaa tatcttccag gatgatcttt aatactccat cctgataatc ggattgaata
     1201 taatcctctg caggaaagga atcctgtgca tggctaaaac tgattgttcc ttccatataa
     1261 acttcttgct cttcgctcgc ctgtaagaat aatctggcta agcgattttc aataactact
     1321 ttgttcaagg gttcaaaaac ccaccttttt tctaaattaa tcttgttcat ttttctctcc
     1381 tttgtgtaat catatttatt gcctcgtcta cattgataaa accctcttcc aaatcggata
     1441 gaacatcaat aaccttatcg gtttccggcc gctttttagt tatgatccca ataatttctg
     1501 ctaaccggtt ttttactgta ggatacgaaa ttcccaattt atcctgcagc tccttcaaat
     1561 taccttgaac taataaaaac aaacggacaa actctaattg gcttgcattt aattctgcca
     1621 gccaactatt ctcaaaatta cccttaaaac taacctcgca atgaggacag tgtaattcct
     1681 tgattattaa ttccttttta caaatagggc aggttgctaa aatcatactc atctccttta
     1741 acattctata gaacaatcta aataatattt aagtaatgtc aagcacaaaa tgagatatat
     1801 taagatatag atgaataata tgtagattta ttgcaacatc tacagtgaat attaagtgct
     1861 gatttaaagt aaagataaac aaacaaatgc cctgaaatgg gtagctattt gtttagtttt
     1921 atataaaaat gatttaccta ttagcagata taaaagcagt tataaacgat tttgcgtgaa
     1981 agttcttgct tgacagaaac tctatccatc aaaaatagtt taaaaaaaaa cgggatgtag
     2041 cgcagaccgg ttagcgcgct cggttcggga ccgagaagtc ggaggttcaa atcctctcat
     2101 cccgaccatt ttttttaata tcactaatat cataaatcaa gaaaaaaggt gcaaaaagtg
     2161 tccgaaaaga aagagtcatt cacatactat cttaagctac tttatccttt cataaagaag
     2221 gatataggtt tgctactatt tgggcttttt gcaatgttaa tcacctctgg tttaaggttg
     2281 ttgcatcctc taatattagc tcagattata gaccacagca ttcctgatag aaatatggcg
     2341 cagatgtttc aatatggggg ttattttgtt attgtagttt tgctatcagg acttttatcc
     2401 tatttgcaga tattgttatt atctcgttta ggcataaaga ttatcacaaa atttaagggc
     2461 aatgtattta gccatttgtt aaagctgccg gtttcttggt ttaataaaca accggttggt
     2521 gaacttattg cccgggtgga aagtgacagt gaaagagtta aagttctttt ttcagacttg
     2581 tctatcacta ttattggcaa tgtgttgttt ttcattggtg gttttatagt tctgttcatt
     2641 ctgcaatggc agataacgat ttatatttta cctgcgatta ttatttgtgc agttggctac
     2701 tggtttttat ttaagtattt gaacgggttt ttccgtttaa ttcgggaacg ctatgctgta
     2761 attagtgcta aaataactga ttatgttcag ggaatccaga tgctgcaggc attaaatcag
     2821 gaaaaaaagg ccttaaaaga tgtggagcaa gcatctttaa gcaaaaagaa agttgaagtc
     2881 catactcaga tgattgaata tggttcgcag agtgttttca gttttatggt aaatgtggta
     2941 tttataatta ttgtaatact tgtatctgca cctaaaatta ttgctggaac tttgactgta
     3001 ggaattctaa ttgtttttat tcaatacatt tatcaacttg tttggcctct aatgcaaatc
     3061 tcagaaaatg taatgcagat tcagcgttct tttgcatctt tgaaaaggat tctggaatta
     3121 acaaaattgc ctactgagga cgaactatat actggaactt tgcatcctgt ttttgaacag
     3181 gaaatccgtt ttgagaatgt atggtttggc tatcaagaag atgaatgggt gttaaaagat
     3241 gtatcgttaa ccattcctaa aggcaaaaag attgcattag taggtccttc tggcagtggt
     3301 aaaacaacaa ctgtaagttt gctatgcgga ttttatccag ttcagaaagg gagaattttg
     3361 attgatggca tccctttaag tgaaataaat ttcagagaat ggcgtagaaa aatagggctc
     3421 atattacagg atgtttattt gttcccgggc agtattttag aaaatgtgcg cgtatataat
     3481 gatcagattg aagaagctaa ggttcaagaa gctatttcca ttgttcagct aaatgaattt
     3541 atcaaaaagt tacctgaagg cataaactcg gaacttgcag aacgcggaca aaatatatcc
     3601 caaggggaaa aacagttaat cagttttgct cgggctctgg cttttgaatc ggaaataatt
     3661 attatggatg aagcaactgc ttcaattgac cctcaaactg aggcaaaaat ccaacattca
     3721 atgcaaaatc tactggctgg taaaacagcg ctggtggtgg ctcaccgtct tacttctgtt
     3781 ttggatgcag atgaaattct atatttcaat gaaggaagaa ttactgccag gggtaagcat
     3841 agtgaattat taaataactc tccagaatat agaaaactgg tggaattgca aatgctacag
     3901 gtgcaagatg aataagcata taaaatggat tttggaacag tataaagcac agaaatggtt
     3961 tgtgctaata atggtagtgc ttactctgct atcttcaatt gtttcaattg cttttcctta
     4021 tgtttttaaa gagatgctgg acctattgaa agatatattg cagtttcccg ataaatatcc
     4081 tgaaccgatg cgagaagttt ataaaattat caaacttcta attgctatcg gtatggctga
     4141 gcttataact gggatctatc ctggaataag aggttgggtt aatatgcggt ttgaacatag
     4201 tttaagggtt atgtacttta agcatatttt agctaaggac ttcagctttt tccagaagtt
     4261 taaaactgga gacatagtaa cgcgtttaac agatgatctt tctgattatc caagggttag
     4321 ctggttccta tgttcaggta tttttcgcgc tttcaattct ttcagtatga tcttgtttgc
     4381 gctggttgtt atgttcagta tcaatattaa actaaccttg ctatcgattg ctcctttacc
     4441 aataatgatg ctggttttct attttacagc ggataaagta tatacgaatt acaatcgtaa
     4501 tcagcaagcg atttctgcta tcaatactca gctggaaatg agcttttccg gaatcaggat
     4561 agtgaaaagt ttcgttagcg aagaaaagta caatcgcttt tttgatgtag cgcttgccaa
     4621 gagatttaaa accgaaatga gtatggttaa gcttaatgcc attctttctt tgatctggca
     4681 atatattgat tacattgctc aggttgcagt tattctattt ggcggttatt tggctgtgaa
     4741 aggagaaata acagttggaa ctttcttcct gttttacaca tatctttcaa tgatgattta
     4801 tccgctgatc gatctacctc aactttttgt tacgggaaaa caggcctttg tgaatataga
     4861 ccgcttggaa gagatgaaga attatccatc gttaaatgat aaatttaagg gtgagcataa
     4921 ggtaaaagat ttctgtgcgt tatcctttga aaatgtctct tttcaatatc ccgataagcc
     4981 atttcctgtg ctgaaaaatg ccagctttaa tcttgatcga ggagaaagga tgcttatttt
     5041 aggtcctact ggaagtggta aaactactat tgccaatctc ttacttggtc ttttaaaaag
     5101 cgatagcggg acaattaaaa taaatggaaa acctattgat gagatagata taaaatcctt
     5161 gcgtaatata gtagcctatg ttcctcagga accattgata tttggtggat ctataaaaga
     5221 taatattctt tttgcggtgg aaaaagcctc tgaaaaagat taccatattg cagtacaaac
     5281 atcccaattg gaaaatgaaa ttgcctcatt tccggaaaga gacgaaactt ttgtgggaaa
     5341 tagaggactt agtgtttctg gtggtcagaa acaaagatta accattgcca gagcattagt
     5401 aaaaaaaccg cagttgctta ttttggatga tattacggct tccctggatg ctgaaaacga
     5461 agaaaaactg tggcaagcgc tggatgaaaa ttatcctggt atagctgcta ttgtaatatc
     5521 tcatcggctt tctactttac gctatgtaga tagtgttctc tttatcgatt cacagggtaa
     5581 tactcataaa ggcactcacg attatcttgt ttttaataac aaggaatatc acgattttct
     5641 gcgtgaacat ttgaaacaag aaaacaaaaa atagcattga cacaaccgca taatttaaaa
     5701 aattgtgata aatagattta aaagggagat gaagatgcgt cgcagttttc tgattatttt
     5761 gctatgtgcc atattaacat ttaattacgc tgatgaattg gatgataaaa tgcgccagct
     5821 gagagaaatg caaaagcaac tggaaagcac tcagcaaaaa gttaaacaaa cacaaagcaa
     5881 aaaacaacaa acagaaaatg aaataaaaag aacttcatca ttaaaaaaac gcacagaagc
     5941 taatcttaac aagcttagat tagacgaaaa aatcgctcag gattccctta aatctgttag
     6001 tgaaagaatt gtagctactg aaatgcggat agcggatatg cataaagagc aaaatacaga
     6061 gctggatatc cttttaagag tagatagatc ttataagaag caaaatataa atcatcgcga
     6121 tcagcgattg ctaaaaacac ttattcaagg taccaggcaa aagattaata tactttccgg
     6181 ctttaaaatc tctcttattc aggttcagga attacatcga aaacaagcaa gtaaactttc
     6241 ccgcggagtt aaagaagaaa gttataaaaa aacaacatac gaaaaaaaga tacgcaattt
     6301 atccactcag agccagaaac ttactataga acaaaaaaag ctgcaagcac aggtggaaca
     6361 attgaaaaaa gacagtgcag aattggaaag tttgattgct caacttatga aacaacaagg
     6421 taaagaacct gccagttacc aatttacggg taaaaaaatt gtctggccac taagaggaaa
     6481 gattattaga aattatggag aagaaaccag aagttacgga accagcgttg taagtaatgg
     6541 aattgatatt gctgccaaag aagggacaaa agttgtggct gcagatgatg gagaagttat
     6601 tttttcagat cgctatggtg ggcagggaaa gctgatcatt atagatcaca aaaacggctt
     6661 tttcactctt tatgcttata atagcgaatt attagtgagc cgcggagcaa aggttaaaaa
     6721 aggacaaacc attgctaaaa gcggaatgac aggttcggca agtgagccat ctttgcactt
     6781 tgaattacgc aaggatggaa gagccataaa tccattacca tatttggagt aaggaggtat
     6841 tgtgcttatt ttgaaggatc ctggcttacg gaagctggag aaatttttag attcaaattc
     6901 tttggaacat tctacaattt tacattggac tggttcaatg tggggaattg gatgtcggct
     6961 ttcctctttg gaaacaattg ctcgcattga aacattaaaa cagagagctg aaaaacaagg
     7021 ttttattgct ctgattcctg atattaatta tttagaccag aaacaaatac cagcagcgct
     7081 gagaccttta atgcatcaat attggcctgg gaatttaacc tttattttcc cctatcagga
     7141 ttctaagttc gaaactatta ctaataaggg aaaggttgct tttagggttc cttccgatcc
     7201 tgctctgaga gcttttatat cattattggg tgaaccttta atcagtacca gtgtgaatgt
     7261 gtctggtctg atgccggaag aaaactatga tcgaattatc tctaaatatg gttcttggtt
     7321 cgattttgct ttattgcccg ctaacaagga aataaaaagc acaacagaac cttctacaat
     7381 tatagaatat atcagtgctg cagatagtgg taataacgat gagattaagt gtctgagaga
     7441 aggctcgata ccgttttatg aaattaagca ggcatttaag ctgccagttg ttatgtttgt
     7501 ttgtacagct aatatatgcc gttcaccgat ggctgaatat ctctttcgga aaatggccag
     7561 agaaaataat atctcccttg ctactgattc ctgtggcttg cttccaggag gacagatgat
     7621 ttctgtaaat gctttacagc tgcttttgga acggggaata ttggatgcac aacaacatat
     7681 ttctcaacaa attactcctc aactggtttc ctccagctgg ctgattttaa ctatggaaga
     7741 aagacagcgt gatttattac gcacaaccta tccgaatagt gaaagcagaa ttaagactct
     7801 taatgaaatt gtcggtgaaa aaggagatat tgaggatcct tttggccgaa atttggaaag
     7861 ctatagaaag acctgtgaga ttattgaaaa ccgtttgcaa ctgctaaccg aatatttgaa
     7921 aaataacgaa ttaaatatac agaagaaaat tatatgaaag attatgaaat aacccctaaa
     7981 gtaatcgctg agcataatct cagcgatgaa gaatatgaac gaattacaaa tatcctgaac
     8041 cgtgaaccga ctattgtgga actgggtatt ttcagtgtga tgtggagtga acatgccagc
     8101 tataaaaact ccgttttatt gcttaaaacc cttcccaagg aaggttcttc tttgctggca
     8161 aaagcaggag aagaaaatgc cggattgatt gatatcggac acggattggc aatttctttc
     8221 aagattgaaa gtcataatca tccttcagct ttagaaccct atcatggggc tgcaactgga
     8281 gttggaggta ttttaagaga tatttttaca atgggagcgc gtccaattgc tgccctaaat
     8341 tctctgcgtt ttggcaatcc gggaaatcct aaggttcagc atttgctaaa ggaagttgtt
     8401 cgtggtatag cggattacgg aaattgtttc ggagttccaa ctgtgggagg agaagtctat
     8461 tttgatgata gttatgaagg caatccttta gttaatgcaa tggccgtagg aatagtaaaa
     8521 cataataaaa ttgcccgttc agcagcctcc ggagtaggaa atgcagttct tattgtgggt
     8581 gccaaaacag gacgcgatgg aatccatgga gctacttttg cctctgtaga tttaagtgaa
     8641 aaatcagaag aaaaaagaac tgctgtccag gtgggtgacc catttctgga aaaactatta
     8701 ctggaagcaa ctttagaagc tatcgatgag gagcttgtag ttgccattca agatatgggt
     8761 gctgcaggca tcacttgttc tacttctgaa atggctggta agggtaatgt aggtattgaa
     8821 attgatgttg ctctcgttcc acaaagagaa acggatatga ctccctatga aattatgctt
     8881 tctgaatcac aggaaaggat gctggtaatt gtaaaaccgg aaaatttgga acaaatcaag
     8941 tctattttta cgaaatggga acttgatgct gttcagatcg gaaaagttac tgatgataaa
     9001 atgctaagag ttttctatgg ggaagaaaaa gtagcggaaa ttccggctga aacacttatt
     9061 ttaggtggtt cagcaccagt ttataaaaga gaaagtaaat atcctgaata ccttgatgca
     9121 ctgcacaatt atgatataga gaaccttcct gaacctgata cttatcagga agttctatta
     9181 aaacttattg ccagctccaa tatttccagt cgacatcaga tttatcagca atatgatcat
     9241 atggttcaga ttggaaccgt tgtcgagcct ggttcagatg cagctgttgt tagaattcca
     9301 gatacagatt tagcaattgg acttagcacc gattgtaatg gtcgatattg ttatttagat
     9361 ccttatctgg gagctttatt agctgtagca gaatcagcaa gaaatgtcgc ttgcagtgga
     9421 gcaaaaccaa tagctattac caattgcctc aatttcggca atccatataa accagaagta
     9481 tactattgtt ttaaagaatc cattcgggga atgagtgatg cctgcaaagc tttaggaact
     9541 ccagttactg gaggtaatgt atctttttac aatgagaatc cggaaggggc aatttatcct
     9601 actcctgtta ttggaatgtt agggttaatg acagattata ggaaagcaac aacgcagtac
     9661 tttaaacacg aaaacgacat aattgttctt ttgggctctt taaaagctag cttcggaggt
     9721 agtgaatacc taaaaataca gcataatata attgctggga agccgcctat tgtagatttg
     9781 gaagaagaat tacatttaca ggatttaatt ttggaactaa ttgataatgg attagttaat
     9841 agtgctcatg atatatccga agggggcttg cttattgctg ttttagaatc ttgttttaat
     9901 cctgagcagt tgtttggagc taagctatct tttgatcttg agctgaatcc tgctaaaata
     9961 tattttggag aagcagctgg cagaattctt attagtgcag cgaaagataa gtacaattct
    10021 attaaagaag cagctatgaa acacggaatt gaatatcaca aaataggaat tgtttctgct
    10081 aattcaagtt ttatcgttaa taatcatatt aacgaggata tggcgatcct acactctgcc
    10141 tggaagcagg gaataatata aaaaaaggga aaggaaatga aaatgaattt tgtttttagc
    10201 aatttctttt ggggattatt ctttattctc ttaggactat ctgtattttt aaaggcgttc
    10261 aatatccatc tacctttagt gagagttttt attgccatta tcattattat gattggtatc
    10321 aaactgcttt ttggcttaaa atcgcatcat tatcataagc caaaatctta ttctaaagaa
    10381 aattccattt actatagcag ttccagagac gaatataata taatcttttc ttccgggatt
    10441 atagacttaa gcgatctgcc ggaaaatgcc aaagatttcg agattactgt agtatttggt
    10501 tccggaacag taattctacc tgataacatt gccgttaata tcaatcctac ttcagttttt
    10561 ggatcaacaa ttttacctcc caaaaatcct gtaacgcagg ataaacctgt tgagattgaa
    10621 tccacggtag tttttggacg cttggaatat aaatatgcca aagctgaaca aacaacagaa
    10681 caaacaactg agccccaata aggaacatac aaatataaaa ctgactttat ggaaaaaacc
    10741 aattatcctg ctgttgctat tttttatttt taatatttta acagcagaga aacttggcta
    10801 tgccttaagt ggtggtgggg caagaggttt tgcacatatt ggtgttttaa aagttttaga
    10861 agaggaagga atctatccag attatatagc tggaaccagt attggagctg ttattggagc
    10921 tctttatgca atgggttatt ccgctaatga tctggaagat atgtgtttaa cactgaattg
    10981 gaatgaatta atcagcgata ctcaaaaacg caaagaccta tatattgggc agaaaagatg
    11041 gacaccttat gggaatgtaa tctttgatct gaatgaatcc tggatacctc aaattccgtc
    11101 ttctgttttt gtagctaata atcttaattt agagcttttt aaattgacag cagcatcctc
    11161 tcaaataaac aattttagcg atttacctgt gccttttatt tgtaatgcta ccgatcttat
    11221 aacgggtgaa ggtgttactt ttacaaaagg atctttgatg caggctttgc gcgcttccat
    11281 atcaattcca agtttgtttg aaccctttga actaaatggt cacctatata ttgatggagg
    11341 tatttctcaa aatatgccaa ttaacctcct ccacgaaatg ggagcggata aagttttggg
    11401 aattaaagtt aattctactt tgcgcaagga agaaaacctg aataactttg tggatatttt
    11461 ggatcaaact atcaacatcg gcattacccg taatttgaac gaacacttag atgtatgtga
    11521 tcttcttatt gaaccagatt taactaattt tgcttccact gattatcaac atatacagag
    11581 tatcatagct gccggagaag aatcagcaag gcaaatgatt gatcaaatac gggctttcaa
    11641 aaaagagcta tctctttctc ctgagcggat aattaatttt gataaacatc tggataattt
    11701 tccagttgcc aatatatttg tgtatggaaa taatacaatt agcgctgcta aagtgagaga
    11761 atatttgggc ttggaaacag ggcataccta tacaagcgaa caaataaaaa aagcttgcat
    11821 caatgcctgg aattcccaaa gttttaaaac aatttaccca gtgctggaaa aaagaaacga
    11881 tgataaatac aatcttcttg tttatgtaaa agaacgcgaa cataaacacc tggctgtgaa
    11941 tcttgtatac aataatgatg aacaactaat ggctggtttg gtgctttctt tagataacta
    12001 tttgctaaag aactcccgac ttttaggaga attgaaactg ggtggcagga atgaatttaa
    12061 tctggattat gttaaaaatt ttggagaaca atggggggca tattatcgac ttttcccata
    12121 tgtaacagaa aaaacaatat ataattaccg tgatcattat aaaaccgata gcgtaaaatc
    12181 tactgaatgg ggtataaatt cgggagtggg tgtttttgct aaagacctcg ccattgctga
    12241 attattcatt tatagtagtc aaactaatct ccatagtgat atctctcaaa caccctttct
    12301 tcctaaaaga tccgtaataa gtggatttgg tgtgaagggt tatcacgaaa gtttggatga
    12361 ttatagtttc ccaacttccg gtgccagagt tttcggcaaa ttcaatttct ctcgatatgg
    12421 agaagttagt gattatctgt atagttgttt tcaaggaagg gcagaatttt atgttcctat
    12481 tatgaatcta ttctctgttt attcagaatt caattatggc agctatctaa attcttctca
    12541 acaaactaat ctcgatcctt ttgtgataag tggaatagat ggttttatgg gatattcacg
    12601 ctatgaagta agtgctcctc attaccaaat tatatctgct ggtatttcct ctcttatctc
    12661 aaaacgcttc tttctggata taggaatcca gagtttacga tattctgatc agaatatttt
    12721 tgagaaccct gaagattggg aatactgcgg atatttagga tttggttaca aaaattccat
    12781 attaccagta aaattatatg tctcgctaaa tgaagataag gatttgcata gttttctaag
    12841 cgtgggatac gattttgata ttttcttctt ctccagaaga taaaacaaga taacttttta
    12901 cagatttctc agattcggta cacccgaaat gaatagaatg aaaggattgg ctggttttat
    12961 gaattgtagg ttgtctggag aactatacag ttaaagcaaa tcgcattata tactttcctg
    13021 aatgacgata aaacagctgg ttatactata ctttaattat ttcgttctct ttatatagaa
    13081 gctctttcga ttattttttt ggtttcctcg cagagggctc gggatttctc tattgattca
    13141 ctttccacat aaatacggat aattggttca gttcccgatt tgcgaatatg gataaaatgt
    13201 ttatttccaa tagctttaat cccatcctga gtatccaatt gatagtcctc aaaaaaggac
    13261 ggtatattct gcatcacatt ttccatacgg gaggcatcca aaattagttt atccttggca
    13321 aaataatatt tgggtaagct atcaactata gcagaaagcg gtttatcctg ttcggcaagc
    13381 aaacctaaaa tcaatgccat accagcaata gcatcacgcg tataatgaac atcaggacaa
    13441 ataattccac catttccttc tccgccaatg ggtgatttta ttgcctgcat cttttttccc
    13501 acatttattt caccaacttt tgttcgatga acctgcacgc caaaaagctc agctatatca
    13561 tccgatatca ttgaactgct aagattaact acaatatctc cccgtttttt gggtagtaca
    13621 aaaagctctg ccaaagctac gcttaattct tctccgatac attttccttg ttcagaaaca
    13681 agggaaaggc gatctacatc aggatctgta gcaaaaccga tatccgcttt gtaaaaagta
    13741 actgcttctt ccaattggtg taaattttca ttcaaaggtt cggctgtatg ggcaaaaatc
    13801 ccggttgctt cactatttat ttcatataca gtgcatccca aaccttctaa tagatatgga
    13861 gaaattaaac ctccggcacc atttacggaa tctaacacaa ctttaaattt acgggagcga
    13921 atctgttcta aattaagata tgggatagcc aaaattctat ctatatgctc ttgaatagca
    13981 ctgttatcct ttgttatttt tcccatagaa tcccaggcag cccagataat ttcattttca
    14041 acggaattca aaaaatactc ggctttttca gggctcaaaa acattccatc ggcatctaca
    14101 aatttcatag cattccactg aggaggatta tgagatgcag taatagcaat gcctccaata
    14161 gcatctgatt cctgaacttt taatagcaaa gtaggtgtag atactattcc taaatcagtt
    14221 acatcacaac ccacacttat gatggcagaa attatgctat gcaacatagc ttgtccggtt
    14281 gtgcgactat ctcttccaac gataatatga ggtttttctt caggataatg ccgtttttga
    14341 attaaagcaa aatgtgctgc atattttatg gctataatag gattaagggt gtccgcaaag
    14401 acacccctaa ccccactaac actggtcatc aatttattca ttaatcacat tccgtatttg
    14461 gcacggttat cgattatttt agcttctttt cgcatttggt cataccaacg gttccaagca
    14521 gcatcttcca gacgcttacg aatttcttcc tgtttatctt tatctttagc gaaagcatta
    14581 aaatcgggct taattcgctc ggaacaactg ataataaagc ttccttcttt agtatgaatc
    14641 aaaccgctgg tttttccagc ttccaatgct aaagcagcat tggtaaattc ttcgcaaagg
    14701 ccaacattgg gaacatattg tcctttcttg tggttgttca aatctattat ctgccagccc
    14761 tctttagctg cagcagagaa atactcgcca gaaggatact tggcaacgaa ttcttctgct
    14821 tttttcttaa tattagttac tttcttttcc ttttccagct ggtatttaat acgaagctta
    14881 accgattcaa aatcttcgta atagaccttt ttgttatcag tgagctgagc gataatttta
    14941 cggttttgct ggtctgtaat aatgtccgat acagctttct ctttaccttt acgcataaac
    15001 tgtaaaagag ctccattatt tccaattcca ggaatatatt cctgatcgtg aggcacccaa
    15061 tcagtttttt gaacttcaac tttcagttct tttgcagcgg catctattcc ttttttcttt
    15121 attaaagctc tagccgattc aactttatcc agcagcgcat ttttggtctc atctgaggga
    15181 tcaactttta gcaaaatatg gctggcttta attctgtaat tttcaggatc ttgggaggca
    15241 atgctatcac aacggataat atgccaacca aaattggttt taacgggctt actgatttct
    15301 tttactttca gagaaaaagc taccttttca aattcaggaa ccatttgccc ctttccaaat
    15361 tcgcctaaag aaccaccatt atctttagaa ccgggatcat cggaatattc tttggctaaa
    15421 gtagcaaaat cctctccttt aataacacgg ttataaactt catcggccaa taattttata
    15481 gaggcatagt ccttgtctga gggcttttct tcgaaagcaa tatatttcat tctcgaagct
    15541 ggtccttttt taaattctgt ttctttgttc ttatcataat aggctttaat ttcagcatcg
    15601 gtaacggtta ctggatctga tttattggga tcaaaccaaa taaattttcc ggaaacaata
    15661 tcagaatcct tcgcatattc tgcttttaga ctatcaatag taattccagc ttgagctttt
    15721 atcttatttt gcaatttttg acgaggaaga taactacgca catattcttc catagcagca
    15781 aaaaactgag gattattctt caaagcagaa agatatttgg atctatcaaa tctgccattg
    15841 gtttgtaagg cttcattttg catcagttcc tgaggtggat tattctgcat ttcggttaaa
    15901 atctcatcat cagtaatttt aattttatgc ttctttatct gctgttgcca caaaatatcg
    15961 ttcaccaaag attcccaagc ttgattttct attgctttac gggtattttc gtctataggt
    16021 tggtccttat acatttgggc atagcgatca tacatttcct gtgttttctg ttggaacata
    16081 tcaaatgtta ttttggtacc gttcactttt cccaggtaag gttttggcga tagtaattcc
    16141 cctaacccaa ttgcacccat acctacaata aacacgaaag caacaatgta gataatcact
    16201 ttctgtttct ttctcaactg atccagcatt tccattctcc ttttgtagag gataaatttc
    16261 ttattttgaa agccattatt ttaatagtgc agtttttaac aaggtttttt ttgcacttct
    16321 gccgatactg tgttttctat tccccgttaa gaaatttatt agtagcgcaa ttatataatc
    16381 tataatattg ttatctttat tatcctacgg ggatgagagc ataattacga ttggtttaga
    16441 acactgtgta tgaggttaat aaagaagctc atttagaaat atcagaatga agcttataaa
    16501 aaaatattca ggtttcctga taaaacagtt ataccataat agttttgtat gttctgttta
    16561 tatcttgaaa atttctcccc aggccagtta ttcggttcaa taattctatt ttagttaaag
    16621 tagaattatt gacccaacac aaattcaaaa tttgcagata aataatgata gaagatcatt
    16681 ctctagcttc aagataaggc aaaatatttt atcctttaag atcgattatt cgaagctatt
    16741 aagatttaat tagaccaaga atatcagcaa aatacaaaga ttatttctat ctaaacctta
    16801 ctttctcttg caatttaaca atatacaact tgtcatccac ccaagctttt ctcatcctta
    16861 accatgggtt gaagaggaga ggaggatgag aggaggatgg gttgaagtgt cctaaaaaga
    16921 acgaaactgg agttcaatga agataagacc aggctggtca aagtggagag tgatcccttt
    16981 gattgttcgg gcttcacttt caggtacaat aaagatctgt tcgtcaagaa caagaaatat
    17041 ccgaatatca taccacgaaa gaagagcgcc caaacaatcc gagataagat cagggagtat
    17101 ttgagaaaga atagacacac gaatccaact gagcttgcca aggggttaaa tccaattttg
    17161 aaaggatggt tgaactacta tggtattctc acagtgagct atccacagct ggaaaaaaga
    17221 aagctcagat agtatatgat ggaatttctc catcgatatt atcgagggaa aagccagcgt
    17281 aaaagtaagc tctatcgcga gaatgctttt gagttattga caatgacatc aaacttccgg
    17341 aatttccgtt gccaggtttt taacctagcg caaacataca aaaaaaatcg ttgcacatag
    17401 cagaggacaa acctgagggt tttgggtaag ctcaaaagaa aaaagattaa aaaataccta
    17461 tattcttgat aatttctcca aatcagacaa aaatcatcga gataatgatc ttgctcctaa
    17521 ttgttcaatg gccttacaac gatcataatc ggaaaaaggt tttcctaaaa taatatctct
    17581 agctgaacct cccactaaat aagtgcattg ctcaaacagc gttcctaaga ttgtagaagc
    17641 aaattagcat gtattttgtt gtgcttgaga ctaatattta aataagttat gatttatgct
    17701 aagaatttac taactggcag ggtttctgta aaattcgtcg cagtttatat tcattttctt
    17761 ttactccatt aggagttaaa tgttcaattt ccagatatct ggttttctca gatgaaggtt
    17821 tatggtcgaa atctaacata catttcagtt gctttaatct ttctatatct gtccatggat
    17881 cattgatttg tatattaaga ttatcattta ataattgata atacgcctgt aaatatggac
    17941 taaaatcctc tatttccttt attccttcat ctttaaggtt agtataacgc ttttgtctat
    18001 caattaaatt taatttggca tcaatcctta tgcttcctaa tcctaatggt ttggccattc
    18061 caattttatg agcacaacct tcaggcaaat tcaaaatgaa aagtaaagcc cccaattctg
    18121 tgtcacttaa attagtaaat cttatccgtc ctttaaaatg agaatttttg tccaagggct
    18181 gaattttagt atactgagtt ttgttctttc taacatcatc aggattagct gtccagtcat
    18241 tagaatcgcg atgccaatag aatttaaatc ctgctaagca tgcattgggg gaatcatagt
    18301 tctttaatct atttatttta tttttctcca aattgcctat cttaggatat tgctgaatat
    18361 aatactggaa agaagtaggt ttgggactgg aaagaatttt aggattttgt tcctttagta
    18421 ttttatcagg tgtgtcacat atcgcatctt caaaatatac tcgtccaggt aatatatcat
    18481 tatcttttat ataaccgaaa acagcttctg ccatatctaa ttccttgctt ttgtgctttt
    18541 ccggtctaag ttcacctata gaatgttgat atagcttttt ctcttttaca cctctataca
    18601 tcaatttttc attctcatct ataaatgtca tcttggaata ggttacaact tcatagaggg
    18661 ttctaatcat acctcttaat gaagaaccgg gaattctaaa ttcagcatca gcaggtttat
    18721 aaaagccact taaggataat ttaaaatctg ctaattcagc taaattgtta ctattttcca
    18781 attcaaccaa ttttttttct tcttcctcag taagcgtttt acgaatgtaa attgggctta
    18841 gagctgtaat atctaaatca atataaccat tattattttt atattgattg aagggaggaa
    18901 tacatttagg ttcagtagta aaaataacat catttaatgg aataaagtta tagggcgcag
    18961 ttgcagtatc aactgaattt tcattatcag gattagaatg agcttgatga taattaggat
    19021 ttttgttggg ttgttgggtt tgagttaaat caattaatgt tgatcctatt ttgattttga
    19081 ctggtttacc gttcatcaat tcatactcaa catcatacac accgtcggcg agtgttttcc
    19141 atacttcatg gtttcgaata tatctttccg agatagtgac tatctttcca tcgttttttt
    19201 ctaattctaa gcagataatg ttctttttag ttgtcctttt attgattttc cctttttcca
    19261 ttattcctcc ctatctgttt caccaattct tacaaagcgg tgatcaaaat aactaagttg
    19321 tccattatcc cagtgttcca aatagttacg agttagcaaa gtaacacgag aattgtctgg
    19381 gtaattctta atccagctat aaggcaaagt aagttggaag ccgcgagctt ctcggataat
    19441 agaatatccg ctaacttgtt gtccattggt tttacccatt aaaatttggc taacatcttt
    19501 ataataagta tgttcgcctt tactatcggt tcgaactctg cctcttaaaa aagtgcttgg
    19561 gggagtactg ttaatatcgt tagttctgta gcaatataat tcagtatcct gattaaaaat
    19621 gcgaatggct tctatattat tatattccaa attgttattt agcagttgaa aaccattata
    19681 atagtcacca aaagctattt ctgaaaggag ataaacaact acttgatact tagtatcttt
    19741 cttcagatta ttaaagatat cttccaaact ggttatttta tttagtagag ttatttcgga
    19801 gttgattgat tgaacctcat tcatttactt ttcctccttt atctttataa atagcaaggt
    19861 tattttgaat ttggtcttta tcactattct tgattaaagc ttccagataa ggagtaaaat
    19921 tatcaggtaa ttctgttatt tgagagatag agtttttttt attttggtca aaaatagttg
    19981 ctttaagacc ttgtaaagtt cctctaccga tgtttttttc tcctccaata gctaaatctc
    20041 ctgtatacag gtcttttaat agtaatataa gtaatccttt ttgcgaaggg agagggtgtt
    20101 ccaaccgtaa tgttaagtgc gtaatattct ctgtcttact gtcttgaggg aaaacaggca
    20161 tagaatcaaa taaagcacct tctatagtcc cgccggtaaa acggtctata catattctgt
    20221 tttgaagttc agtggttaca ttgctaatag ggacttcgtt tacatataaa gcagaaggta
    20281 ttgtatattt cttattctta gtaacagcag ttccaaacat tgaggaaaca aaatcctcaa
    20341 taaaatccac atcctgccat atagtgtata gaattctttc tgcctgggct cttaatactc
    20401 cgcgaactga tgttgcagga agaataggtt ttccttggga tgatatatga actgcatcgg
    20461 ggccataaac atcttcggga taagttcgca cgataagcga attttttaaa tgaaaatcag
    20521 ctttaatgac gaaagtatcg taagttgaga atttgggctt ataattttcg atgttgttac
    20581 tactctgaag ttctaataaa tactgagtca gtcctgatgc cgtatcaaaa tcatattcat
    20641 taacatataa gttaccagct ttcagtttac cgaagccaga acttcttttt ccacccagga
    20701 aaaatgaatg ttcaaatact ccgagacact cctccagaat atgttgtgct tgtatatatc
    20761 tattttcatt gttaataaca aactccaagc ggaaagtaaa atttaaacct ggctccacta
    20821 cttgataatc aaatttggct ttatcttcta ctatgccttt actattatca atgcgaattc
    20881 catcccgaat cctacaattt cctttagaag agcaaggata taaatcatca caaattaaaa
    20941 aagattgatt tacaggttcg gataagtaca aaccgagata atccttatca atgtctacat
    21001 tgaaataagt ttttaatact cccaggaagg aagtagctgg aatgaaaact ataccttcac
    21061 tatcaactaa cacatctcta tcagaaatat catctcttcc actacctatc attaaaggac
    21121 tcaataaatt aagagttcca cccagaataa ttctacctgt tttcattggt cctccttgat
    21181 ctttgatttt ctcgcctaag acataaaaac aatgctttga ggaagatctt ttttgcctta
    21241 tctaaagaaa atttatcaag ctcaatttca gcatatacat cattaattat ctttttatca
    21301 ctaacatata gctgtttggt attttcgaga aatgtggata ctattgtatc aatactcatt
    21361 aagtaatcgt aaaagttctt tttatcttct gttaagtaga cctgttttag tttatcttgt
    21421 gctaactttt tgagttgaat gatactttca ttaaaattac catgttcagc aaaagattct
    21481 aatcttccta ataagctatt gttgatttta taatgtttaa tagtccctgc tttgtcattg
    21541 gctacttggg ttaaataagc agtaattatt tgttcaatgc ttttactaag aatgccttta
    21601 ataatatcag gtggatttgt tgctggtttt tgaacagaat tcatagttgt agagataatt
    21661 tttaatctgt ttgttgggtt cttcataaat ttgatttggc caaaaccctc gttacatctt
    21721 tcgcctaaac catataaaag aagattagag aaattctcag gcattttttc tagcaggaaa
    21781 caagaacctg ctttgtaaac caattccgaa gattttttag ctttgtaagc attaacgact
    21841 gtttcaattc taccactaat aatactggaa tggaggattt taacccctag gattttttct
    21901 aattgattag tttctatagt gctgcaacca taatcatttt taatgatagt atcagatatt
    21961 aatacaagca gaggattatc agaagaagca aagttttgga tattatgttc ttgtatttca
    22021 cattccttta ataaacaatt accgtattga gcagttttag atttacccag gcgaaaacta
    22081 agattatttt ctaataaacc tttaatcaat tgtaaatcta tttcatcacc atatatggtt
    22141 ccaacaaaaa cttgttcagg agaaagggct tcataacaaa atacaatact ttgtttggga
    22201 attcctgtag cataatctct ttcgtgatgg aagaagattt ccttgtgagg ttgatataac
    22261 ataatatcat catcatcatt aacataggcg aaagattcta ttggtctata atccagatcc
    22321 attgcttcgt tattaaaaat gttaagaact tttttattat tatttttgtt ttgccagata
    22381 ttatgtgggc aaggtaagta tatatgtgat tggtattctg ggtagagatt atgaaaacat
    22441 aaacctcctc ttataaacaa gcggtagaat tctggcatat caccattttt tttaaggtag
    22501 ttgcttgcca aaactcctaa tatcgaggtt cccggaatgt aagatagagt tttggtaagg
    22561 gtactatctc cggattggtg agcaataatt agaggtgatt ttaattcaat cttgagtttt
    22621 agtgctttat acatttctcc ctccaacttt cagcaaattt agcagttaga ttgacattat
    22681 tatccaaact tattaaatcg attttaactt taccgaatcc acgattacgc atagttccta
    22741 ttctctttat ataaaaacag atagcagcta aatatgtttc aacttcttga gagggggcat
    22801 tgaggataag tgctccttca aatgtaaacc ctctttttag tactctactc gtccggagag
    22861 aatgttcttt agcaatatga gtatctttat caattgcagt ttgagcgcgc aattctgtaa
    22921 aatactcctt aatgatgaga gtattagcta aataaggaaa ttgttcggtt aaataattca
    22981 accatatagc aatattatcc ttatcctgca attccagatt ctcaaatata attttaggat
    23041 tgggatcatt ttctccaata ttgccaaatt taagatcggg gattcttgtt agctcagtta
    23101 attctttaaa acctttaaaa ttagctaaat cctgagctgc attacggaaa agtcctttaa
    23161 ttctttttgc agggatataa ggtaagcaat ggctgtcaaa tacgatatcg ctatcaataa
    23221 ttgttcctga agatttacca gaaccaatca gcgtatcgga gagggttttt aaaattatct
    23281 tgtaaatcat tattttcctc cagaggaatc atttccatca gttctatcat atctaagtaa
    23341 atagtttgat tattaaaaaa caatgtatcg ctataatctt caaaccctgt aaattttata
    23401 ggcaaatcct cgcgagaagt tatttgcttt aataaagcaa ttaattgatg ttcggattta
    23461 tataaggcag agcgaagttc ttttattttg gagtttggcc attttgaata catatcatat
    23521 gttgcgttta aaagttgttc taattcagat aaagaatatg gcctgcgagt taaacttttt
    23581 tcttgtggta gaatataatt tttacttcgg atttcatcca agggatcata tacagaagca
    23641 ttgatcaaat gaaaatctaa ataactatca ttattattgt tagttaaacg cttattttta
    23701 gcagacctgc ataagctgga agccatctga tatacttgat agaaaggata tttgtaatta
    23761 gcaacggcaa tacctgcaca aaaagatagt agaaggtcat cgctatcctc tttgatttgt
    23821 ttaataaatt caatagccaa aggaattcct aaacgacctt tgcaaacaaa agtaatatca
    23881 tcacctccaa taaagagagg acgcaaaggt aaatacttgc ctccaattga ggatgtatca
    23941 tttaaggcat tctctttctt tttagattta ttacattttt caacggtttt gctgacaata
    24001 gctttaaaag ctttaaggca aagctcatca attttattgg atagctttat agtagaatcc
    24061 agatcttttt gttcagcgaa taatttacct ataccattgc catcaatatg gactacagct
    24121 aggtaggatt cattgccttt atcaatacct aaatcatcaa atttttcagg aaagatatag
    24181 gaatcactca attcagcagc gtaaagttgt ttagtttctt cttcagcctt tttcgaagct
    24241 atcctttttg cgtatacaat ttcggagagc aattcatcct ctctggagtg atcaatttct
    24301 actgctgcat atcctgaata attacattga gcattaattc caaaagatgg tactgtagtt
    24361 tgttggatac gatgttcttt ttccttgcgc aacttttctt gcagtaaact gaaagcagcc
    24421 gggaaatcat ctagattaaa attctcttca taaactgcgc taaaacttaa accagggcat
    24481 tcatttaaga tttttcgtga gagtgaatag atagtttcct ttgcttcttt ttcatcttta
    24541 cagagcagaa aaagattacc acctccacaa taaccctggt cagttaggaa ctcctccaga
    24601 agatgcttaa ttaaatacga agcaccaata ttatccttaa ggcggttaga ggcaaaaata
    24661 taatcctgaa ttgctctaag atcaataaat aaaccataag ccataattac tccttaatat
    24721 aatttataat agctgggaaa atagatttat cttttaattg tgtttctcca aaagccctaa
    24781 aattgggcac taaagttccg atatattttt ctacatctct ctggagatta tctggttcat
    24841 ccgtacgcga aataagtatt gtttttgctt cttctcctcc taactgcgta gctcttgtaa
    24901 tggcttcaaa ggcctttaac ttgcacaatg gttttgtttt atctgtagta acagatatta
    24961 gggtaagttg gtagccataa attgcaacta tatctacctg atattgatta ttgtgaacat
    25021 caaaataaaa ttgaatattt ccagtgagtt taataagttc attgtagaca taatattcca
    25081 gccatctacc attaatgaaa tcttccaaat cttctctttg gtttctattt aattcctcgt
    25141 actttatatt tacactttct aacaaacgat ttttttctgg tgttatgttc aattgattgg
    25201 ccatatgttc caggtcatta ttccttttcc catagatttc ttctaaattg ccattatcta
    25261 acaagtcaat tagtttttcc aataattcag ggtatttttc tttatattcc tctggcttaa
    25321 tgtctgtttg attttgtaaa actgccatag tttctaaatt tatcgccaac atagggtctt
    25381 ggcgtaaatc aatttgcgaa tctgctacat taccatcaat aatcataatt ttgcgtctgc
    25441 tatctaagta tgaagaacta aaagatttgt agtggctttt tagataatag taacaaaatg
    25501 ctgccatagt tttagtcccg ccagtaaagt ttaaatgaat tgctttttta tcttcttcgt
    25561 ttatatattt atttaacttg cattcaattg agttgaaaga agttacatca tcaatagaaa
    25621 ttatctgatt aaaatcctct tctttaaatt tgccttcctt tataagttga gttttgattc
    25681 tttgcccgat atcttctgta cctgtttggc ctaaggtctt attttctttt gaacatatta
    25741 agctaatcct ggaattattg tagtggttct ttaaataaat tgcacttaca taacatggaa
    25801 ccggattggt acctaccagt attaaaactt caaccataat tagcttcctt tcatattagc
    25861 atatatagct tattttgaag aaaagataaa ttctgtacaa tagtgtttag atagaattgc
    25921 acttatctgc cactatccta acaaaacata gtatattaat agaaacggac tctctaacta
    25981 tattatatat catagaacta tataaaacaa ttataagtat tatgtcaagt gataaaaaga
    26041 aaaaatcata atttattgta acagtatagg aatgttaata ttcaaccgct taaaaaaacc
    26101 tatgctactc atactgtgag acgatcataa ttatctatgt ggcctgtagg caaatgtata
    26161 caagttgtta aagattttca gtttttttta attggtttat agtacaatat gctaaagaaa
    26221 tatatgcaaa agcagtgatt atacagattc atatatttat gagagtgctt attttgacat
    26281 atttgctaat attacttgga atggagaatc ataataaatg aagagtcagg cagctgaatt
    26341 agcatattgt gcaaaaacat ttcccttgac gaatggaggg ttatcagagt tttggcttaa
    26401 taatgtaaaa tctttaatat gataaaaaat gtataaggga gcataaagct aatgaagaag
    26461 ttttcctggc gtggcctttc tatagtcata tttatatgcg tgacagcatt ttttttggct
    26521 cctttggcaa ttcctaatct accaagctgg tggtcaaaac accatttgaa actcggtttg
    26581 gatttaagag gtgggatgca gatacttctg gaagttgata cttcagagct ttctgcaact
    26641 gatgccagaa atgctgttga gcagaatatt aagatcattc gtgaacgtat tgatcagttt
    26701 ggagtagccg aacctactat ccaaaaatta ggcgagaaaa gaattatggt tcaactgcct
    26761 ggtgtttccg attataaggc agctgagaat ttaattaaac aaactgctat gttggagttt
    26821 aaacttgtag ctccgtcaga agaagctaag cgtgtaatag atgctatcga taaaaatatc
    26881 agtggtaatc tggataagtt tccagctttg gcagaattag ccaagaagga taaaagcatt
    26941 attgctgata caacttccac tgatagctta caagtaggaa ccggtatttt cagttcgttg
    27001 attcgtcctg gtgaaatgga ttttgaggtt caatatagcg atgtgcgttt aattcaagaa
    27061 ctgatttctg atactttatt tgctcaattt gtgccaatag gttatcagct tgctttagat
    27121 aaagcagata ccgaaaatcc ccgagcagat agagctttat atgttctgaa ttcagctcct
    27181 cagcttacgg gagcagatct tgcgaaagct aaagtagaat atggttcagc cacatctacc
    27241 gatccgcgag ttgccaataa accctatatt tctatagaaa tgaaaagaga aggggctcgt
    27301 aaatttgaac gcgttactgc tgataatgtt ggtaagcgtt tagcgattgt tttggatggt
    27361 gtagtttatt ctgctccgaa tattcaggaa agaattgctg gtggtagagc tcaaattaca
    27421 ggaaggttca cagctactga ggcaaacgaa ctggctattg tactcagcac tggtaacttg
    27481 atcgctccca tcaaaccagt atctacttct attattggtg ctactttagg tagtgatagc
    27541 attcgtaaag ggacttttgc tggtttaatt ggtttgataa ttgtagcctt gtttatggtt
    27601 atctattata aaggatgcgg ttttattgct gatattgcct tggttttaaa tgtgggcttt
    27661 attttagcta tgttaactgc gtttggtgca actttaaccc ttcctggtat tgcaggtata
    27721 attcttacta ttggtatggc agtggattcc aatgttctta tttatgaacg aataagagaa
    27781 gaattggata tagggaaagc tcctcgtaca gcagtagatg caggttttaa gagagctatt
    27841 attacagttt gggattcaaa cttaactact cttattgcgg caattgtgct ttattatctt
    27901 ggaactggtc caatccgtgg ttttgctgta accttagcta tcggtattat gagttcaatg
    27961 tttactgcca tagtgatggt tagagccata ctggataatt ttgttcttac gggaacaaaa
    28021 acaaaactga gcgtgtaagg agttattata atgcgaatcc tcaagaatcc taatatccaa
    28081 tttgtgaaat atcgttactg ggcttacgct ttctcaatcg ttgtgctctt gatcggcttg
    28141 ggaggtatta tattcaatgg cttgaattgg agtatagatt tcaccagcgg tatagccgca
    28201 acattgaact tgaaagctaa agatccaaat gtagctcctc ttcctattga tgaattacgc
    28261 tccgttcttg ccaaaaatgg cttcccggat gcggaaatta tgtatgttgg cgatgaagcc
    28321 aattctgttt tccaaatcaa aataaaaaca acaggtaaag gcagtaatat ttcagcggaa
    28381 gctcgatcga aattgattga tatcatccaa agtgaatttc ctgaatatgt gaaaggtcga
    28441 gatatcaata aagaagttat agaagaaata tatgaagttg gtcccaaagt tggtagcgaa
    28501 cttcgaactc aagcattttg ggcagttctg cttgccttga ttgcaatgat catttatatt
    28561 tggttccgtt ttgaattaac ttttgggcta atgggaattt tggctttatt ccacgatgtg
    28621 cttattattg tgggtatttt tgctcttacc ggcaaagaaa tcacaatgca aattattgcc
    28681 gcgcttttaa caattgtggg ttacagcatc aacgatacga ttgtgatctt tgaccgtatt
    28741 cgcgaagatc tgaaaaagaa tcgtaaagac cctatgccgg ttatattcaa taaatctatc
    28801 aatgaaaccc tttccagaac aataattaca ggtggaacta cttttattac ttctgcttgt
    28861 ttatttttct ttgggggtag tgtgattcac gattttgctt ttgccatttg cttaggtatt
    28921 ctttttggaa cctattcttc aatctgtgta gccagcaacc tggtgataga tttacatcaa
    28981 atcacaggga aagaaaagaa aactttgcaa cacttaacta agaagaaaaa ataaccccat
    29041 acaatctacc tcctctcgaa ccgctcatct tttgagcggt tctttctttg atcttctaca
    29101 ccgagaacac tgaacaaaga taacgacacg caaatagaat tttgagagct atcgaaagta
    29161 ccgaaagaaa caggagcaca gaaaaaaaat aggaaggaaa gaaaaggtag aaaagtaatt
    29221 tagattcttg ttacttgctt tcaactctta gctttttgcc ttcaacttgc tctatggctt
    29281 ctacttatag cttccagccc attcttgttt cacttttaca cttttttgcg gaggaacggc
    29341 gtcctccggc tacacaaaaa acagaggaat agcgtcctca aactacctgt aagatttgca
    29401 cctaaaacta tctccgggtt tcacttttcc actttccacc ttcctcttct aactatgaac
    29461 ttcaaaaaag ctctccagtc gcagcagtgt tctggcatcg atatcttcag gttgatcacc
    29521 ttccagagtt aaaaaaggca ggtctatata ttttcttaga agcaggttat ccaactgtag
    29581 atggcaaaaa ctttgcgtat agctaataac agcttctata ttgcgttttt caagttcggg
    29641 ctttatatct ttcaaccggt caaaaacact atagggataa gtataagcaa tgtattgttg
    29701 aacgatatcc ggtagtaaat aaggcatcgc aaattgacgc tgcacttcgt tgaaaataac
    29761 atcccctccc agatcaataa tcgtatcgta tatgttttta tagataggag gcactcccaa
    29821 atatgccaga cgcagttttg ttggcagggg gtctcttttt tctgcttctt tcaaaaacat
    29881 atccaattca ctttcgtagc gatctggatt tcccccaaaa tcggaagaat taacctgcca
    29941 aaggtgattt tcaagcccac taactaagcg ttctttccaa gtccattcat ccaggactaa
    30001 tagttttttc cgaatctcgt ctaatcgctt ttttgccttc aaagtatcgc ttcgagacac
    30061 accaaaataa ttttccagct tggttatttc tttatctaaa tcctcagggt ttctattagc
    30121 aggaaaggaa aaacggtata tcggtatatt aacttcagta aacatatcca gtagagaatt
    30181 actattggaa caatcaccta ctacaattcc gattatcata tccagattag aagatagcgc
    30241 agctatataa ttacctttaa tccagctgca aagagtgcgg gggaaacctt tcaattcagc
    30301 attctgaaca taattggaag aatcctgtga tataaataca ttgttcaaat ctacaggaat
    30361 atggcctgca gcgaataaaa cttccacagg aaaggaagta gtaaatccaa tgcgtttagg
    30421 aatcatagct aagctccaga tatttatttt ccaattcggc aatgcgttgt ttgcttttat
    30481 ttatgttgct aagcagcagt tcaatttgct ggcatagttc ttttgctcgt aattcattgc
    30541 tataggtgga agaaagtgcc aattcagaat ttatgttttc aatttttata ttactttctt
    30601 ctatggttgc ctgttcttgt tcaatttgtt tatgaatctg ttctagatgc caagggttaa
    30661 ttttcttttt tctttcgcgt agcacaggag cttttttagg cagctctgga atggaaaaag
    30721 caagctccaa agcttgttcc agacccatat ccacttcgct aatagtaggg tataacaagt
    30781 cgttttccaa tgccttatga aacacccaat atttagtggc cagttgcttc ataaaatagc
    30841 gatcgtgact aacaaacaca attgtgcccc gatattcttg taaagcagat agtaaactat
    30901 tattcatcgc tatatccaaa tgatttgttg gttcatccaa aattagcaaa ttgggtgttt
    30961 gatgaattaa tgtacaaagc atcaatcgcg atttttcacc tccgcttaga acacaaactt
    31021 ttttatcaac atcatctccc cgaaaaccaa agcgagctaa aaagctcaat acataaccgc
    31081 gggtttcatt gggaaccaat tgccagagag tgtccataac ggttaattct tcatccaata
    31141 aaacctggtg ctgatcataa tacccgattt ctaaagaagc gccaattttt agcgaaccag
    31201 aaaaaatatc tttttcttcc ataagaattt taagcagcgt tgttttcccg cagccgttag
    31261 gacctactat acaaatccta tcgcgataat gaacttccag attaacattt tttgctaaag
    31321 gtgcattctc aaaaatgcct aattctacat cttttaaagt ataaacatca ttaccactgc
    31381 gtcctgttga ttggatattt agttttatct tcttttgctg ttgagggttt tgaataactt
    31441 ccattcgtga aagcaacttt aaacgagatt ttgcctgagt agttttctgg ccggctatgt
    31501 ttctacttat aaaatcctcg gtttcggcaa tgaatttttg ctgtcttgca tattgccttt
    31561 cctgggtttt cagttcaatt gcttttgctt gttcaaaaga gctgtaatta ccttttgtta
    31621 tgcttaagtg tttattttct agatgaaaga tcacggaaac agtattatct aaaaaagtac
    31681 ggtcgtgcga aaccactaaa aaagggatgt tactggcatt tagatatttc tctaaccagg
    31741 aaatcattgc tacatccaga tggttagttg gctcatccaa aatcagtaaa tcgttagaca
    31801 tcatcaatat cctagctaaa caaatacggg attgttctcc tccactgaag agatttaagg
    31861 atttaggata atcgtcttcg gtaaagccaa gagaagttat tatgaatttt aagtcgtttt
    31921 caaattcatc accaccaatt gcctggtact tttctaaggc atcattaagc tccgtctcat
    31981 ttgcttcaga atgattatct tgaagcaatt ctgatagctc atttattttc ttctgtaatt
    32041 tcagaatatc tggtcgagaa ctatgaacat agtttataaa agaaatattg ccatcaagaa
    32101 ctggattttg tggtaggtaa gcaatccggc attttttgga atgtaacacc tttccttcgc
    32161 tgggcagtaa aacacctaac atcaatttga taagtgtggt tttaccgctt ccgttcgaac
    32221 cgataagccc tattttacta ttatgctcca aagtgcaatt gatatcactc aatatatagt
    32281 tgccgccaaa ttcaatgctg gcattgataa cttgtattaa gctcactttg taatattcct
    32341 gtatatttta tttaaaactc ggaaaaacat atcccaagct gaattaaatc tactttagga
    32401 aaacacttta gctgtcaacc tcattttttg cttgtcaaaa aaccccatct aatttttaag
    32461 gttaataaat ggttcgggat gtagcgcagt ccggctagcg tacttgaatg gggttcaagt
    32521 ggtcg
//
LOCUS       DAOSTH010000014        27986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_1879_length_27986_cov_13.162579, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000014 DAOSTH010000000
VERSION     DAOSTH010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 27986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27986
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1879_length_27986_cov_13.162579"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..744
                     /locus_tag="PLB65_03595"
     CDS             <1..744
                     /locus_tag="PLB65_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOQ77641.1"
                     /translation="ELALKYKPSVTLAGATVIGRSFIPRVSINLHTGLTADCTGLSID
                     PDTGDLLQTRPAFGGNIMATIRTANHRPQMATVRHKVMNPIPRDDSRNGIVIQEQVNF
                     DLDEERTVWLGFEKEKTNLINITEANIIVSGGRGLKESKNFALIEELANALGGAVGAS
                     RAAVDSEWIAYSHQVGQTGKTVKPLIYIAVGISGAIQHLAGMSSSDYIIAINKDPDAP
                     IFKVADLGIVGDLFEIVPKLTKRIKEQRV"
     gene            741..1952
                     /locus_tag="PLB65_03600"
     CDS             741..1952
                     /locus_tag="PLB65_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOQ77642.1"
                     /translation="MTIRPSISVINPDRVEQGVDLHLWGTPFQELELHQDFPDAIAYI
                     PLINSHDHLIGNWVPRAGDHRPYQNSHIWVEDMKDSFAFRERDLFWNNDGNFHLEEPN
                     ALLLAQLGAYKNLFCGCSVVQDHSPLQVDAYYKAMPIIVTKNFRQCHSITLGNWWGGE
                     NPQKEMQLTQGKMPFIVHLAEGIDEVSKLEFAEVRKLGLLKENSLFIHCIALQEDEIA
                     DIAKAGASICWCPSSNFYLIGDTLKIEAALANKVNVCIGTDSTMSGGINLIAEFLTIR
                     EHFPFIPVKEIYRMATENAAKALYLGKDYASINPDKCNNLLLVDKIDRDPFENLYTME
                     ASTIQLLLVDGIPRFGDSEWMELLNLDEDKYTTFRTGNKEKFVIDDPIDLNEQIDEVL
                     GYHKDFPYLPF"
     gene            2115..2744
                     /locus_tag="PLB65_03605"
     CDS             2115..2744
                     /locus_tag="PLB65_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ77643.1"
                     /translation="MEKHLNVTDIFYSLQGESTYSGYPCIFIRLSECNLRCSYCDTQY
                     AFGKGKSMAISSILEQIKKYPCSLTEITGGEPLFQEDIDALFEALHNAKYDILLETNG
                     SISINNVPDYVIKIVDVKTPGSGMENSFLLANLQFLNTQDELKFVITDKVDYKFALSF
                     IKQHNPSVKAIHFSPVTEVLNASELSKWMLEDGVKAKLTLQLHKIIGLE"
     gene            complement(3015..3227)
                     /gene="thiS"
                     /locus_tag="PLB65_03610"
     CDS             complement(3015..3227)
                     /gene="thiS"
                     /locus_tag="PLB65_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HOQ77644.1"
                     /translation="MCNESVFYVNGQKLPWKEGLVISDALKMMNYTFKMLVIKLNGEL
                     VKRENWQTTSIPQNADLKVIHLISGG"
     gene            complement(3231..3848)
                     /gene="thiF"
                     /locus_tag="PLB65_03615"
     CDS             complement(3231..3848)
                     /gene="thiF"
                     /locus_tag="PLB65_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS adenylyltransferase
                     ThiF"
                     /protein_id="HOQ77645.1"
                     /translation="MISREEYFSKRDPYAFPIWQKATVGIAGLGGLGSNIAVSLARAG
                     IGKLILADYDIVTLENLNRQQYNISQIGKLKVDAIAEYIHSFNPFIELILHSVLITPE
                     NFDSIFDEADLLLEAFDEAEQKEMLISCWTSSFPNKHIIAASGIAGYGKNETIHTEHY
                     GFLHIVGDLESELTEGISPIATRVALIANVQANLALELLASSKGY"
     gene            complement(3845..4594)
                     /gene="mobB"
                     /locus_tag="PLB65_03620"
     CDS             complement(3845..4594)
                     /gene="mobB"
                     /locus_tag="PLB65_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOQ77646.1"
                     /translation="MKALAIIGYHRTGKTTVATTLIKELTKQGYNVCSIKDIHSEAFR
                     CDTEGKNTYLHTQAGSQAVFAKGLYDSALLFPKSLDLKDMVSYLKSDYLIIEGLKNAP
                     VPKIVCAENTEQLNELVDDTCIGVSGIISSKIDSYKDLPVFCLPEDLPKMLATVLDNC
                     FDILPLAEPECCSACGKDCYSMAGDIVQGRAKRSDCVLDNKAKLKLWVGEKEIVIVPF
                     VQKLLEDIVVAFVNNLKDIEPQGDIKLEITR"
     gene            complement(4597..5073)
                     /gene="ispF"
                     /locus_tag="PLB65_03625"
     CDS             complement(4597..5073)
                     /gene="ispF"
                     /locus_tag="PLB65_03625"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOQ77647.1"
                     /translation="MIRSGFGYDVHRLVKDRELILGGVKIPHSKGLMGYSDADVLLHS
                     IIDALLGALALGDIGTHFPDNDLAYKDIDSRLLLRKTYILISGKGWKINNLDATICCQ
                     EPKLKPFIYDMRKNIACDLNCDLEQISVKATTEEGLGLSGKGEGISAFCQLLLIKV"
     gene            complement(5077..7761)
                     /locus_tag="PLB65_03630"
     CDS             complement(5077..7761)
                     /locus_tag="PLB65_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOQ77648.1"
                     /translation="MRIPIPVFGFGCGVLGLALPKVSLNMGVYASKLLKSLEYRGYDS
                     TGAAFQGDTTEITLLKDVGAPSTLVKTLGIEKQSGKIFCGQVRWATFGFVDKINAQPH
                     EVNCKRHIYGAHNGNITNTGELKSFLLNQGHFVQSDNDGEMLVHIIEHYFDIEMDKTG
                     DPKAPEERKNCMRQAIIQAANKLVGSYAAVIVDPDTETSWAIKAGSSLYFGIGTIEGM
                     PFSLASSDLTAVLRFTKQLVNLREGEFIEYTAENYQVYAQKDLKFKRLNQPNEIWKTG
                     DQIPTKPVYSKLRAEDVELLPEYEYFMEQEIYAQSETTGKLIKLFQGGSNTGKRMLAL
                     MDKEGVRDFLQEQVKQFLDAHLPEERQKVFYDLLNSETFSNFFNQVRSTYKEFFDVAV
                     KEDFDKKYFFSSEKNLFLEMANNGYDLKKISLAKTMDSIAEEMNVKDFNDSIEDFLLL
                     AKNTILNSRNAYSIACGTSFHATKIAALFFNTIAGMEIIPILPGDFRGEYSNCIKDND
                     LIIGVSQSGETKDLIDIFNDIDAKNLNVRKVVLVNNMNSTLGQEKSDVAIPILCGPEI
                     AVPATKSFMNQITLFYYLAIRTAQMKLNEIDTHFSTEQREKHQQELDGYFHSLSKIPS
                     LIKETLENVSGELDIMAGKLYMEPSIHILATKISGVAMEGALKIRETVLTHAEGREAS
                     EFKHGPNTILGKNTVFGVKHLRSLLHYFSENIDAVESLCEQEAIPHKEIKDIYKALAE
                     YIFTHNQPFNLSLRGTKVFNKIVKDKDFFEPLYRNYPLIYVTGPNERDVNLTISQINT
                     HKIRGANTFVIAEENEQLMKNVASRPSENSYYAWSYIMLPKTGSTMMTCFSASAVLQL
                     LALKLSIRKMKRLDKLEVRDHGVHPDVPKNVSKSITVD"
     gene            complement(7833..8537)
                     /gene="ispD"
                     /locus_tag="PLB65_03635"
     CDS             complement(7833..8537)
                     /gene="ispD"
                     /locus_tag="PLB65_03635"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOQ77649.1"
                     /translation="MDFLKNTTAIITAAGSGKRLPGSSKKQFRELVGIPILIRSMEPF
                     MASELIDNIIVTVPEEDITKTEALIEQWFEGFTKPYKIIPGGAERQDSVFSALKACPE
                     GTEYVAIHDGVRPFVTLELIELLFAAVTVDKAVVPASKLKHTVMQIEDNYAVRTINRN
                     QLVNVFTPQVFEYALIMEAYIAAYRNNFYSTDDASLIQNLGERIRVLWSDDFNIKITD
                     EADLFFAKQLIEKNKI"
     gene            complement(8525..9538)
                     /locus_tag="PLB65_03640"
     CDS             complement(8525..9538)
                     /locus_tag="PLB65_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ77650.1"
                     /translation="MPITLMMAVGNRIYNLEPHFKMIKYKHLFGPVMSRRLGISLGID
                     LVPYKYCPLNCVYCEVQKTTHQVTKRESYFEVREILDELDNFMATAPHLDYITFSGAG
                     EPTLNSSIGEIVNYIKAKYPSYKLALLTNGVLFNDKEVRQEILPCDIVLPSLDSATQE
                     GFEKINRPCPELKVQDLIEGLVQFRKEYKGTIWLEVFLIKGINDTEEEIEALAKAINR
                     IKPNLVQLNCLDRPGAEDWVNPIPLHNLKQIKAQFKEKTSVPVEIIAKVKYKTTESQL
                     DTELLEMLHSTLIRRPSTAEDLSAMLDIHINEISKVLRQLCLEKKITVKRESRGVFYI
                     WTS"
     gene            complement(9477..11729)
                     /gene="bamA"
                     /locus_tag="PLB65_03645"
     CDS             complement(9477..11729)
                     /gene="bamA"
                     /locus_tag="PLB65_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOQ77651.1"
                     /translation="MQRFILCSILILLLGCFAQNLYAAGETIYEIKVIGAENIDPELV
                     TSALSIRVGDSLDSEAVAKSIRSLYRMGIFSDIQIETEPYRTGVTLLVKVQENPVVSS
                     IEYIGFKTIKKERIDELVSVRVGSYWSEGVKNQLLRTLKNEYASKGFSNAEIQIFESH
                     GAKNKIGLKVQVDEGKRVSIKQITFVGNNYFDGKTLQKKMKTKTSSFLRSGRLEQEKF
                     DADLQTLTAFYKKNGFIDVVIGPYEIQPLNDKAIEIVINIYEGTKYNFGGINIEGNEF
                     FPIEELKEAFTMKIGEPFDQEIFDNEMRIIYGKYFDEGFIYASIVPEYVKEGDQLIVN
                     IKINENNRARIRQIHITGNRRTKDKVIRRQLEVSPGDYFRQNKVIRTQQNIYNLGFFE
                     QDIRLDYTPVNSEGDIDLQFDVMDRASGTANGGVGYNSQDKFVGQLSVAQNNLFGNNW
                     STNLTWEFGGNTQTFEFAFTNPNLMDTDILLGTNIYYTKKTWSSFHYEIFTRGAGLRV
                     GQTVPWIDKTRAVIGYSLYSKKYRITNMDAIMADSSSNANLIELNKLSWRYTSATSLT
                     ISRDTRDNVFFPTKGSQFVIFSEIAGGMFGGDFDYFKQIAQVNWYMDTWEKLILRTKW
                     RFGYVTAYGRSKDAPPDEKFYLGGTGADGIRGFPDSSIGPSGGGTRAIIFSTELGYPL
                     GSDQIIAVAFFDAGNSYNKMRDFNFLNFKKGTGLGIRIRSPFGLIGFDYAYNINDGGW
                     EPHLQFGTTF"
     gene            11973..14831
                     /locus_tag="PLB65_03650"
     CDS             11973..14831
                     /locus_tag="PLB65_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HOQ77652.1"
                     /translation="MLYGQKTQDFKPGRKCSFPQTYTNTEISRADSAHGFDVQKYEIT
                     LSINDAFHNVFGNVLATVQATENLSSIAYELKGLNVTSVKVNGINTNYTHSLGLITIP
                     LNVIAGEQFTTQIFYSGNPVLSNDAYHIGMIFSSNTVFTISDPDAARYWWPCYDHPWD
                     KAIVDLHITMRSDWKVASNGIRTAIVDNGDGTSTTHWSGQNPMTTYLVCITAGPYVEI
                     EQTVPEQNNLPIQNFVMQNQYNNALIDLQNLPLMISWFSELFGDYPFEKYGNAVVSMS
                     TYGAMEHQTMTTLGNYIITGTGYYETVIAHELVHQWFGNAVSFLTFKDVWLSEGFATY
                     SEFLWIDKTSGWQSACDYMRSSIQQYYLNWEIAEGPQTIYNPSFNNYFSPPSYEKAAS
                     VLHMLRLKLGDADFFALLQQYYNTYKNSNAITAEFQNIAEQVSGQNLSYFFNQWIYGS
                     GIPNIQYSLWQNEISNNLEIYAKTTSPTVTSFTVDIPFRISQNGISDSLLVIADPDGY
                     ANDYNYPIISDNMEVEANYNNWTLLNDLTEQIPRFTECLPSNDCVMLFWNSFISEKDY
                     QYQLYRKLSSEQEWTLISTMDNNTFSYADTTVSGGFNYDYRLRVLDINGYFSKFTDTI
                     TITPQSFSFANEFLVVDETKDGNGFTINPDDEMVDSFYSAALGGFACPIDNWDCATQG
                     LPDLATLSNYKLVLWHADDFSQNLLQDNLSVISSYIAGGGKLVLSGWKTASNLTTEFL
                     DRFAGGVTPVYDASPCLISAHSDIYNNLTVDPDKTIVSWNSMLPYIYTFNNAAEPIYT
                     ANMIDGSNGAGQCIAFKHDFNGTFVMFGFPLYFMQYPEVRIMLHQLLPTLNPALPITD
                     ETVAMPEVSISVMPNPFNPSTTISFYLPVSGYIECELYNLKGQKVTTLAKEKMTAGKH
                     SISFNGCDSQGRNLASGVYFLRLQHPNGVINKKITLLK"
     gene            14840..15934
                     /locus_tag="PLB65_03655"
     CDS             14840..15934
                     /locus_tag="PLB65_03655"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOQ77653.1"
                     /translation="MFYCPTRIIFADDAIQKAKDKIAVLGKKALLVSGEKSAKQSGAL
                     SDLITVLNELEIHYVLFDRIKENPTLDSVMEGKQIFLQNNCDFVIGIGGGSPIDAAKA
                     ISLAAANSLNRDEIYNIAAFKNVYPIMAIPLTAGTGSEATQYSVLSDPTHKKKAGLGS
                     DLAFPALAAIDPKYTLTLTPKVTMHTALDALSHLLEGLYSNQRSPLVYPFIYKGIASI
                     MKCLPRALSEPDNLAARKELMQSALYGGIVIAQGSTTLQHSIGYPLTSNYNIPHGLAN
                     AMVMEDIMELYYPAVSKELNDLFAYLNLSKDAFYAWLRALPFERKIELSDTFIEEAIP
                     QVMGSRNMALNPLPVSEEHIRIIFEHIRKS"
     gene            16427..18544
                     /locus_tag="PLB65_03660"
     CDS             16427..18544
                     /locus_tag="PLB65_03660"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ77654.1"
                     /translation="MEEKEIKLLEKALSGKPTKDNFYQGLKLVSYYIGCNPHKAEEST
                     QILLQMANLMGTAEEIAFANKTMGSVNSVLGNHQEAIPYYQKAVNLFSQIGKNLQMAQ
                     SLASIASMNTTLGNTAQALKTFMEVIPLFEQENDQHSIAITYSNMANIYKAKMDYAQA
                     IQYYQKAADLAESIQCLDLQVSSLCIMGSIYSSLMDYNKNLACCLKALTLAESTGNNY
                     LIGEVHCSLGVAYQELQDYDKALEYYYKSLKIGREQQIPYYISGNLSNIADIYRKQGK
                     LKEALKAYKEALKYDLQTGDIYNQSASLRTIALTYIDMHRYALARKTILQALNIISAD
                     EHLEMGINLQADLLLTCIKQQDYKYAEALLKKLLKKVQDNPVSEVQISIYEYAAGLYT
                     KLRNYKKANYFLNLHSQLYKEFINQEREKINRFMQISFETARLQDEKEMLQFQNELLE
                     KRVQAELNKIKEKDKLLAQQEQLAMLGKISAGIAHEINNPLAAIKQIVEITLEDLNSL
                     QPRGYLSQKKNDVLQLLERINRLVEIIKVIARSPERFNANSFSLNQIIEEFIELFGDK
                     IISKDVLLIKDCEPDLPPVYADNIRFIQLLSILATNSNDALKNVNRSEGKYITISTKL
                     IDGRIMLVVEDNGCGMDQETLSRATEPFFSSKEIGQGTGLGLSLAHSIVLNSKGNMTM
                     ESELGKGTSVSLSFPVEDHYEKQ"
     gene            18531..19922
                     /locus_tag="PLB65_03665"
     CDS             18531..19922
                     /locus_tag="PLB65_03665"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOQ77655.1"
                     /translation="MKSNDLTHILVLDDETSIRDGLIHYFSERNYRISGASNWEEAQL
                     VLTKSIVHIIILDVVLPGFDRKKDGLDVLSLIKQKYPDTEVIMISGHATVEKVITAWN
                     QGALMFLEKPYSLEEMEKAVKRAEYYSRGFSRLHQLEAQVISQKMEQSLGTKVIAKSP
                     QMKKCLSLAKKYAQNNHSVLITGESGTGKEIVAKLIHYFSNRSEEEFVAVNVNAIPHE
                     LFESTLFGYVKGAFTGANTNTTGLVEKAGKGTIFLDEIGDLPMEQQVKLLRLIETREY
                     YKVGSSRPSRTEARFIFVTMQDLNQKVEQQSFRNDLFYRISDLEINIPPLRERKEDIS
                     ALCEYIIPQLAKEEGIAKIPVLTKDAYKAILTYSFPGNIRELKKILRKAILNLESNEI
                     LKEDLFPPVEQNVTKTKGTFCPDCEAYFIKKVLTNTDFNASLTMKLLGISRSSFYDKL
                     KRYNINVKHNIEE"
     gene            complement(20205..21125)
                     /locus_tag="PLB65_03670"
     CDS             complement(20205..21125)
                     /locus_tag="PLB65_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HOQ77656.1"
                     /translation="MPKVLIATEKPFAADAVQKIKTELENAGYNYSFLENYTEKSALH
                     NALADAEALIVRSDIIDEAAFNAAPKLKIVVRAGAGYDNIDLKSATAHNVVVMNTPGQ
                     NSNAVAELAIGMMIYMARNKFNGKSGTELAGKTLTLYGFGWVARYVAKIAKGIGMDVY
                     AYDPFISDEVIQKEGVKPLHSVEDIFKLGDYISLHIPATEDTKKSVNWNLLSLVKDDA
                     ILVNTARKEVIEEDALLKAFAEKKKFRYVSDVAPDNSAKLLELYPDRVFFTPKKMGAQ
                     TSEANTNAGIAAAKQIIAYFEKGDVKYKVN"
     gene            complement(21163..22290)
                     /locus_tag="PLB65_03675"
     CDS             complement(21163..22290)
                     /locus_tag="PLB65_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOQ77657.1"
                     /translation="MHELSQFIQTTGFLNVTWGNLVMIISGMIFIYLGVAKEFEPLLL
                     IPIGFGIIVGNMPGVSQQGLGVMNEGSVLNYLYFGVNKSIYPSLIFLGIGAMTDFSTL
                     IANPRLILLGAAAQFGIFATFIGSLLLGFNVMEAASIGIIGGADGPTSIFLASKLAPH
                     LLGSIALAAYSYMALVPVIQPPIMKLLTTKKERLIRMKPLRVVSKKEKIIFPIVAFVV
                     TTFISPGSVVLLAMLFLGNLLKESGVTERLANSARTVLIDVVTVLIGLTVGAKTTAEV
                     FLTPATLKIFFLGAFSFAAATAAGVLFAKIMNLFSKNKINPLIGSAGVSAVPASARVS
                     QVVGQQYDKTNYLLMHAMAPNVAGVVGSAVAAGVLLGILGK"
     gene            complement(22295..23008)
                     /locus_tag="PLB65_03680"
     CDS             complement(22295..23008)
                     /locus_tag="PLB65_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HOQ77658.1"
                     /translation="MKTYKLLINNEHFEAKILEYSPTHARINLNGTEYLVQIENDKID
                     SVPKLAAQEKAVPLAPAFSSNADLFSGEIRAPLPGVIVTIPVSEGDEVKAGQSILIIE
                     AMKMQSEIASPFDGKIEKILVKERAPVKEGDLLMKITGVDIKSPPISTSPKHSPEKES
                     EKTGNKDKILRAPLPGTIMDIKVQVNDYVKEGDIVLILEAMKMESDIYSPRTGKVSKV
                     YVHKGDLVQDNDPLIEFED"
     gene            complement(23005..23850)
                     /locus_tag="PLB65_03685"
     CDS             complement(23005..23850)
                     /locus_tag="PLB65_03685"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HOQ77659.1"
                     /translation="MRKIVFLAIFACSFLSAFAQIDLIEQTISDSLALVREKEILSEY
                     GFRDTATLAEVAEKLQIKDIASWKKYLGLEPYNEKLDKMSLRKLGITPYKALLAKQYS
                     VYGFTELSTLDEVASVLNMPIKKIKQKIGLEKPNSQQWDSYSLQSLNVTPEDIIKIEQ
                     EFNEQSMNYGLNVTLVGMLTVFTALLITSIIVGQLNLLNRPPKKEKADLKISPGGKVR
                     AASATLNQNVIAALVTAVMLHEQTLEDQRKIALTFKRTPTNQWRANAMLNMPNREMNN
                     RRRYR"
     gene            complement(23861..25411)
                     /locus_tag="PLB65_03690"
     CDS             complement(23861..25411)
                     /locus_tag="PLB65_03690"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOQ77660.1"
                     /translation="MENQNPLEKLRAKRAAALLGGGEKRIVEQHLKGKLTARERIALL
                     VDPDSFEEFDMFVTHRCTNFGMERYKHPGDGVVTGCATINERLVYIFAQDFTVFGGSL
                     SKTYADKICKVMDMALKNGCPIIGLNDSGGARIQEGVDALAGYAEIFWRNVMASGVIP
                     QISAILGPCAGGAVYSPAITDFVVMEKSNSYMFVTGPKVVKTVLNETVTNEDLGGAKV
                     HTSKSGVAHFIANSEEDAMLTIKLLLSYLPSNNLEDPPYVPNADPITRNCDVLNNLVP
                     ENPNKPYDMLEVIYPIVDNGEFLQVMSNFAQNIIVGFGRMNGRTVGIVANQPKILAGV
                     LDIDASIKGARFVRFCDSFNIPLVVLEDVPGFLPGTMQEHNGIIRNGAKLLYAFAEAT
                     VPKVTVIIRKAYGGAYCVMNSRHMRADIVYAWPSAEIAVMGPEGAVEVIFRKEAMASE
                     DPKAILQAREKEYREKFANPFVAAERGYIDDIIEPSATRFRLIRALEMLANKQDSIPP
                     RKHGNIPL"
     gene            26061..26183
                     /locus_tag="PLB65_03695"
     CDS             26061..26183
                     /locus_tag="PLB65_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77661.1"
                     /translation="MNNLSNLHNLHNLRNLRELFPVALVAKYSSNILAYKVNIS"
     gene            complement(26369..26749)
                     /locus_tag="PLB65_03700"
     CDS             complement(26369..26749)
                     /locus_tag="PLB65_03700"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77662.1"
                     /translation="MNMTKIYILAECIFLLIMLMGCSLATKPESTADESTANDNCLME
                     VYPNPVVKGETVVVNFNFHLAGLYKLVITNVLEQEVISFDITDNSLIWDGRDKYGQLC
                     ETGVYYITLYSDELVLSRKILLFA"
     gene            complement(26770..27930)
                     /locus_tag="PLB65_03705"
     CDS             complement(26770..27930)
                     /locus_tag="PLB65_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77663.1"
                     /translation="MKKIILLAFILGVVIANLAAISNYVLMVSCFEPSAINLALGGSP
                     VGVVNIWHNEPLNAYDNPAYPSLHTGLSYSNTCYDFMRLKHPGDEKESAYYASMMSLS
                     YKGFGFLFPSVTNVHTWGINADYGSIAFVDENGYEIGITKQLKEFMYPLGISVNFADL
                     YRIIHNDNTFSNKKLDLCLGMNYILNCSSIDIGYSYPANIGEEYDADCVDSGALLKYS
                     FPLTDKIHLESVAGFSMFNAYGDGIKYNSGQQHKIYKRINTGIACSASLKNPNYLKEN
                     ELTDNFENIGCLRLLAGMNDDRTQSPNIYGIGTELGFLDTIFLRSGYHKDKGGNIEGL
                     TYGIGLDLHYRNLVSLNGNYSYFPGGSQCKDKNIYSFGCNLNVFQIASEMLK"
BASE COUNT         8775 a         5433 c         4841 g         8937 t
ORIGIN      
        1 gagcttgctc ttaaatataa gccctcagta actttagctg gtgcaacagt tattgggcgt
       61 agttttattc ccagagtatc aattaattta catactggtt taactgctga ttgtacaggc
      121 ctttccatag atcctgatac aggcgattta ttacaaacgc gtcctgcttt tggtggaaat
      181 ataatggcta ctataagaac agctaatcat cgtcctcaga tggctactgt ccgtcataaa
      241 gttatgaatc caatccccag agatgattct cgcaatggaa ttgtaattca agaacaggtt
      301 aattttgatt tggatgaaga gcgaactgtt tggcttggtt ttgaaaaaga gaagaccaat
      361 ttaatcaata tcactgaagc caatattatt gtttccggag ggcggggatt gaaagaatcc
      421 aagaattttg ccttaattga agagctggca aatgctttag gtggagcagt tggagcttcc
      481 agagctgctg tagattcaga atggatagca tattcccatc aagttggcca gacaggaaaa
      541 actgttaaac ccttgatata tatcgctgtt ggaattagtg gagcaataca gcaccttgcc
      601 ggaatgtcca gttctgatta tattattgct atcaacaaag atcctgatgc acctatattc
      661 aaagtagcag atttaggtat agttggagat ctttttgaaa tagttcccaa actaactaaa
      721 aggataaaag aacaaagagt atgactatca ggccttcgat cagtgttatc aatcctgatc
      781 gagtggaaca gggagttgat ttacatcttt ggggaactcc ttttcaggag ttagaattgc
      841 atcaggattt tcctgatgcg attgcttata ttcctcttat caattctcat gaccatttga
      901 tcggtaactg ggttccaaga gcaggagatc atcgtcccta tcaaaattct catatttggg
      961 tggaagatat gaaagattct tttgcttttc gagaacgcga tcttttctgg aataatgatg
     1021 gaaactttca tttggaagaa cccaatgctc ttcttttagc acaactgggt gcgtacaaaa
     1081 atttattttg tggttgcagt gttgttcaag atcattctcc gcttcaagtt gacgcctatt
     1141 acaaagcgat gccaataata gttaccaaaa atttcagaca gtgtcattct ataactcttg
     1201 gtaattggtg gggcggagaa aaccctcaaa aagaaatgca gcttacccaa ggtaaaatgc
     1261 cttttattgt tcatttagca gaaggaattg atgaagtaag caagttagaa tttgcggaag
     1321 taagaaagct tggtctgctg aaagaaaatt cgctatttat tcattgtatt gctttgcagg
     1381 aagatgaaat agctgatatt gccaaagctg gagcttcaat atgttggtgt ccttcctcca
     1441 atttttattt aattggtgat actttaaaaa ttgaagctgc tcttgccaat aaggtaaatg
     1501 tatgtatcgg aacagattcc actatgtctg gaggaataaa tctgatagca gagtttttaa
     1561 cgattagaga acattttcct tttatacctg taaaagagat ttatcggatg gcaacggaaa
     1621 atgcagctaa agctttatac ctgggaaaag actatgcttc tattaatccc gataaatgca
     1681 ataatttatt gttggtggat aagattgacc gcgatccttt tgagaattta tacacaatgg
     1741 aagcttctac tattcagctt ttattggtag atggtattcc tcgttttggg gatagtgaat
     1801 ggatggaact tctaaatttg gatgaagata aatatactac tttccgaacc ggcaataaag
     1861 aaaaattcgt gatagatgat ccgatagacc tgaatgagca aattgatgaa gttttgggat
     1921 atcataaaga ttttccctat ttaccttttt agggagttct tacttttgtt cgatcccgga
     1981 aattactaaa ggtttgttca tttccgttct accatatctg ggaaataagg gttgtcttta
     2041 tttttgaata accatcgaga tttggttgtt ctcattttat catatttctg ctgacatata
     2101 ataaggataa acaaatggaa aaacatttga atgtaacgga tattttctat tctttacagg
     2161 gagaatcaac ttacagtggt tatccctgta tttttatccg cttatcggag tgcaatttac
     2221 ggtgttctta ctgtgatact caatatgcct ttggcaaagg taaatcgatg gctatcagtt
     2281 ccatacttga gcagataaaa aaatatcctt gttcgctgac tgaaataact ggaggagaac
     2341 cactttttca ggaagatata gatgcattat ttgaagcttt acataatgcc aagtatgata
     2401 ttttgctgga aaccaatggt tcaatttcta ttaacaatgt tccggattat gttattaaaa
     2461 ttgttgatgt taaaacccct ggcagcggga tggaaaatag tttcttatta gcaaacctac
     2521 aatttttgaa cacacaggat gaactaaaat ttgtaataac tgataaagtg gattataagt
     2581 ttgctttatc ctttataaag cagcataatc cttccgtgaa ggcaattcat ttttccccag
     2641 ttacagaagt tctaaacgct tcagagcttt caaaatggat gctggaggat ggagtgaaag
     2701 cgaaattaac tttacaatta cataaaatta tagggttaga ataagacata attttgccgt
     2761 ttttctactt acttcctggt tagtagctat cgtggtattg tggtgctgct gctgggcaaa
     2821 atccccagaa gctctatttt atctccaccg tatgtaacta agggggaaga aggttttact
     2881 gctttgtttt ggttgattcc ccgattttac gaataattat agttcaataa gcatacaagg
     2941 ggataatagg gctatactta gtagctgcag caagagtatc taacaaaaca agttatagat
     3001 gaagcctttc tttatcagcc accgctgatt aagtgaataa cttttaaatc ggcattttga
     3061 ggaatggaag tggtttgcca gttttccctt ttaacaagtt ctccatttaa tttaataact
     3121 aacattttga aggtataatt catcattttg agggcatcgg atataactaa tccttctttc
     3181 cagggtaatt tctgtccatt tacataaaaa acggattcgt tgcacataat ttagtatcct
     3241 ttagaggagg caagcagttc caaagccaaa ttcgcttgta cattagctat gagggcaaca
     3301 cgggtagcta ttgggctaat tccttctgtt agttcacttt ccaaatctcc cactatatgc
     3361 aaaaagccat aatgttcggt atggatagtt tcgtttttgc catagccagc aattcctgaa
     3421 gcagcaataa tatgtttatt cggaaaagat gaagtccagc aagaaattag catttctttt
     3481 tgttctgcct catcaaatgc ttctaaaagc agatctgctt catcgaaaat actatcaaaa
     3541 ttctctggtg ttattaatac agaatgcaaa ataagctcaa taaatggatt aaatgagtgg
     3601 atatattctg ctatggcatc cactttcagt ttgcctatct gggagatgtt atattgttgg
     3661 cggttaagat tttccagcgt aacaatgtca taatcggcaa gaattaattt cccgattccc
     3721 gctctggcta aagaaacagc tatattagaa cccaaacctc ccaatccagc aattccaaca
     3781 gttgcttttt gccagatagg aaaagcatag ggatcacgct tactgaaata ttcctcacga
     3841 gaaatcaacg cgttatctcc aattttatat ctccttgggg ttcaatatcc ttaagattat
     3901 tcacaaaagc aactacgata tcctccaaaa gcttttgcac aaaaggaaca atcactattt
     3961 ccttctcccc cacccaaagt tttagtttgg ctttattatc caagacacaa tcgcttcgtt
     4021 ttgctctacc ctgaacaata tctcctgcca tagagtaaca atctttcccg caggcagaac
     4081 agcattctgg ttccgctaaa ggtaagatat cgaagcagtt atctaaaacg gtagcaagca
     4141 tcttcggtaa atcttccggc aaacaaaaga caggcaaatc tttataacta tctatcttag
     4201 aagatattat accagataca ccaatacagg tatcatcaac caattcattt agctgctcgg
     4261 tattttccgc acacacgatt ttgggaacgg gtgcattttt gagtccttcg ataataagat
     4321 aatcggattt caggtatgag accatatctt tcaaatctaa cgattttggg aatagcaagg
     4381 cactgtcata taacccttta gcaaatacag cttgacttcc tgcctgtgta tggagatatg
     4441 tatttttacc ttcagtatca caacgaaatg cttcactatg tatatcttta atagagcaga
     4501 cattatagcc ctgttttgtc aattccttta ttaaggttgt tgccacagtt gtttttccgg
     4561 tgcggtgata accgataata gctaatgctt tcattattat acctttatta atagtaattg
     4621 acaaaaagca ctgatacctt ctccttttcc gctgaggcca agaccttctt ccgtagtagc
     4681 tttcacagat atttgttcca gatcacaatt taaatcacag gcgatatttt ttcgcatatc
     4741 atatataaac ggttttaatt taggttcctg acagcaaata gtggcatcca gattattgat
     4801 tttccagccc tttcctgata ttaagatgta tgttttacga agcagtaaac gggaatctat
     4861 atctttgtaa gctaaatcgt tatcaggaaa atgtgttcca atatcaccca aagccaaggc
     4921 acctaaaaga gcatcaatta tgctatgcag taaaacatcg gcatctgaat agcccataag
     4981 ccctttggaa tgaggaattt tcacccctcc caaaataagt tccctatctt tcacaaggcg
     5041 atgaacatca tagccaaaac cgctacgaat cataaatcaa tccacagtta tactttttga
     5101 aacattttta ggtacatcag gatgaacacc atggtcacgc acttccagtt tatccaatct
     5161 tttcatttta cgaatgctaa gttttagagc taaaagttgt aaaactgcag aagcggaaaa
     5221 acaggtcatc atcgtggaac ctgtttttgg taacataata taagaccagg cataatagct
     5281 attttcgctg ggacgagaag caacattttt cattaactgt tcattttctt ccgctatcac
     5341 aaatgtatta gctcccctta ttttatgggt atttatttgc gatattgtaa gatttacatc
     5401 gcgttcattg ggtccggtta cataaattaa aggataattc cgataaaggg gttcaaagaa
     5461 atctttatct ttcacaatct tattgaaaac tttagtgccg cgcagagata gattgaaggg
     5521 ttggttatgc gtgaatatat actctgccaa tgccttatag atatccttta tctccttatg
     5581 aggaatggct tcttgttcac atagagattc tactgcatct atattttcac taaaataatg
     5641 cagcaacgaa cgtaaatgct taaccccaaa aacggtattc ttacctaaga tagtattagg
     5701 accgtgctta aattctgatg cttctcttcc ttctgcatga gttaaaactg tttctcttat
     5761 ttttaaagca ccttccatag ccacaccact tatttttgta gctaaaatgt gaatagaagg
     5821 ttccatatat aatttcccgg ccataatatc taattcgccg cttacatttt ccaaggtttc
     5881 ttttattagc gaaggaattt tcgatagaga atgaaaatat ccatccagtt cctgttgatg
     5941 cttttccctt tgttctgtgc taaaatgtgt atcaatttca tttagtttca tttgagctgt
     6001 gcgaatagca agataataga aaagggtgat ttgattcata aagctttttg tggccggaac
     6061 agcaatttct ggtccacaca aaataggtat ggccacatca cttttttcct gacccaaagt
     6121 agaattcata ttattcacta aaaccacttt ccgcacattc aaattcttgg cgtctatatc
     6181 attaaagata tcaataagat cttttgtttc acccgattga gaaacaccaa taataagatc
     6241 gttatctttt atgcagttag aatattctcc tctgaaatca cctggtaaaa tggggataat
     6301 ttccattcct gcaatggtat tgaaaaacaa agcagcaatt ttagtagcat gaaaacttgt
     6361 tccacaggca atagaatagg cattgcgact atttaaaata gtgtttttgg caagtagcaa
     6421 aaagtcctct atgctatcat taaaatcttt cacattcatc tcttcagcaa tggaatccat
     6481 tgtttttgcc agagagattt ttttaaggtc atatccgttg tttgccattt ccagaaatag
     6541 atttttctcg ctggagaaga agtacttttt atcaaaatcc tcttttacgg caacatcgaa
     6601 aaattcctta taagtgctgc gaacctgatt aaagaaattg ctaaaagtct cggaattaag
     6661 taaatcataa aatacttttt gccgttcttc gggtaaatgt gcatctaaaa attgtttaac
     6721 ctgctcctgt aaaaaatctc gcacaccttc cttatccatt aaagctaaca ttctttttcc
     6781 tgtattggag ccaccttgaa aaagcttgat cagcttgcca gtagtttcac tttgagcata
     6841 aatttcctgc tccataaaat attcatattc tggaagtagc tcaacatctt cggctcgcaa
     6901 tttggaatat acaggtttgg taggaatttg atcgcctgtt ttccaaattt cattcggttg
     6961 gttaagccgt ttaaatttta aatccttttg ggcatatacc tgataatttt ctgcagtata
     7021 ttcaataaat tccccttcgc gaagatttac caattgctta gtaaaacgaa gtacagcagt
     7081 aagatctgaa gatgctaatg aaaatggcat cccttctata gtgcctatgc caaaatataa
     7141 actactacca gctttaattg cccaactggt ttctgtatct ggatctacaa ttactgcagc
     7201 ataacttcct actaacttat ttgcagcttg aataattgct tgcctcatac aatttttacg
     7261 ctcttccggg gcttttggat ctccagtttt atccatttcg atatcgaaat aatgttcaat
     7321 tatatgaact aacatctctc catcattatc actttgtaca aaatgacctt gattaagcaa
     7381 gaatgacttt aattctcccg tattagtgat attgccatta tgtgcaccat aaatatgacg
     7441 cttgcaatta acttcgtgag gctgagcatt aatcttatct acaaaaccga atgtcgccca
     7501 acgtacttgc ccacagaata tttttccact ctgtttttcg attcctaagg tcttaactaa
     7561 agtgctggga gcccctacat cctttaaaag ggttatttct gtcgtgtctc cctgaaatgc
     7621 tgcccctgtg ctatcataac ctctatattc taatgatttt aaaagcttgg aagcgtaaac
     7681 ccccatattc aatgaaacct tgggtagtgc taaacctaaa acaccacaac caaaaccaaa
     7741 aacaggaatc ggaatacgca attcccattt cctaataata gataataatc gctttagcaa
     7801 agaatgcaaa aaaaaccaca tcgttatgtc ctctatattt tatttttctc tattagctgc
     7861 ttagcaaaaa aaagatctgc ttcatcagta attttgatat taaaatcgtc actccaaaga
     7921 acacgaattc tctctcccaa attttgaatc agagaagcat catcggtaga ataaaaatta
     7981 ttcctatatg ccgcaatata tgcttccata ataagtgcat actcaaaaac ttgaggagtg
     8041 aatacattta ctaactggtt ccgattaata gttctaactg cataattatc ttctatttgc
     8101 ataactgtat gttttagctt gcttgctgga acaactgctt tatcaacagt tactgcagca
     8161 aaaagaagtt cgatcaattc caaagtaaca aaaggcctaa ctccatcatg tatagcaaca
     8221 tattcagttc cttcagggca agccttaaga gccgaaaaaa cagaatcttg acgttctgct
     8281 cctcctggga tgattttata gggcttggta aatccttcaa accattgttc aataagggct
     8341 tccgttttgg taatatcttc ttcaggaaca gtaactatta tattatcaat caattccgaa
     8401 gccataaagg gttccatact tcttatcaaa ataggaattc ctaccagctc tcgaaattgc
     8461 tttttactgc tacccgggag gcgtttacca cttccagccg cagttataat tgcggttgta
     8521 tttttcagga agtccatata taaaaaacac ctctactttc tcttttaacg gtaatttttt
     8581 tctccaaaca caattgccgc agcactttac ttatttcgtt aatatgaata tctaacattg
     8641 cggaaagatc ttcggcagtg gatggtcgcc ttataagtgt actatgcagc atttcaagta
     8701 gttctgtatc caattgactt tcagtagttt tatattttac tttggcaata atctctactg
     8761 gtacagaggt cttttcttta aactgagctt taatctgctt gaggttatgt aatggtatag
     8821 gattaaccca atcctctgct cctggacgat ccaaacaatt tagctgtacc aaatttggtt
     8881 taattctatt tattgctttg gcaagtgctt cgatttcttc ttctgtatca ttaattcctt
     8941 taatcagaaa aacctccagc caaatagtac ccttatattc ttttctgaat tgaactaaac
     9001 cctctatcaa atcctgtact tttagttcgg gacagggacg attgattttt tcaaaacctt
     9061 cttgcgtagc agaatcaagc gaaggcaaaa caatatcaca gggtaaaatt tcttggcgaa
     9121 cttccttatc gttaaaaagg actccattgg ttagaagtgc taatttataa cttggatatt
     9181 tagcttttat atagtttact atttctccga tggaactatt taaagttggt tctccagctc
     9241 ctgaaaatgt aatataatcc agatgtggtg cagttgccat aaaattatca agttcatcta
     9301 atatctccct aacttcgaaa tatgattcac gcttggttac ttgatgtgta gttttctgca
     9361 cttcacaata aacgcaattt aaaggacagt atttatatgg gacaaggtct atgcccaaag
     9421 aaatacctaa gcgacgggac attacaggtc cgaaaaggtg tttatactta atcatcttaa
     9481 aatgtggttc caaattgtaa atgcggttcc caaccgccat cattaatgtt ataggcataa
     9541 tcaaagccaa ttaaaccaaa aggactgcga attctgattc cgagaccagt acctttttta
     9601 aaattaagaa aattgaaatc gcgcattttg ttataactgt tacccgcatc aaaaaaggcc
     9661 acagcaataa tttgatcgct acctaaagga taacctaatt cagtagaaaa aattatagct
     9721 cttgttcctc ctccagaggg tcctatagag ctatctggaa acccacgaat tccatctgca
     9781 ccggttccgc ccaagtaaaa tttctcatca ggaggagcat cttttgatct accataagca
     9841 gttacataac caaaacgcca ttttgttcgt aaaataagct tttcccaggt atccatatac
     9901 cagttcactt gagcgatctg cttaaaataa tcgaaatcac caccaaacat tccacctgct
     9961 atttcactaa aaatcacaaa ttgactgcct tttgttggga aaaatacatt atcacgagta
    10021 tctctactaa tagttaaact ggtagcactg gtatatctcc agctgagttt attcaattct
    10081 attaaattgg cattggatga agaatctgcc ataatggcat ccatatttgt aatcctgtat
    10141 ttcttggaat atagagaata gccaataacc gctcgggttt tatcaatcca gggaacagtt
    10201 tgaccaaccc ttaaacctgc acctcgagta aaaatctcgt agtggaaaga gctccaggtc
    10261 tttttcgtgt aataaatatt agttcccaga aggatgtctg tatccatcag attaggatta
    10321 gtaaaggcaa attcaaaggt ttgggtgtta cctccaaatt cccaagttaa attagttgac
    10381 cagttattgc caaataggtt gttctgagcc acggaaagct gacctacaaa tttatcttgt
    10441 gaattataac cgactccccc atttgctgtt ccgctggcac gatccataac atcaaattgg
    10501 aggtctatat ccccttcact gttaacaggc gtataatcca acctaatatc ttgctcaaaa
    10561 aagccaagat tataaatgtt ctgctgagtg cgtattactt tattttgacg gaaataatcg
    10621 cctggagaaa cttccaactg tctacgaata actttgtcct tggttcttct atttccggta
    10681 atatgtatct ggcgaattct ggcacggttg ttttcattta tctttatatt tacaattagc
    10741 tgatctcctt ctttaacata ttctggcaca atagaagcat agataaaacc ctcatcaaag
    10801 tatttgccat aaataatgcg catttcatta tcaaagattt cctgatcaaa gggctctcca
    10861 attttcattg taaatgcctc tttcagctct tctatgggga aaaattcatt tccttcaatg
    10921 ttgatccccc caaaattata tttagtacct tcatatatat tgattacaat ttcaatggct
    10981 ttatcattta gtggttgaat ttcataggga ccgattacta catcgataaa accatttttc
    11041 ttatagaatg cagttaaggt ttgtaaatca gcatcaaatt tttcttgctc taaccgtccc
    11101 gatcgcaaaa agctggaagt tttggttttc atttttttct gcagggtttt tccgtcaaaa
    11161 tagttatttc ccacaaaggt gatttgtttg atggataccc ttttcccttc atcaacctga
    11221 acttttaagc cgattttatt ttttgctcca tgagattcaa aaatctggat ctcggcatta
    11281 ctaaagccct tggaggcata ttcatttttc agtgtgcgta gtaattgatt ctttactcct
    11341 tcgctccaat aactacctac ccgaaccgat actaattcat caattcgttc ctttttaata
    11401 gttttaaatc ctatatattc gatactgcta acaactggat tttcttgaac ctttactaat
    11461 aaagtaactc ccgttcgata gggttccgtc tctatttgaa tatcggaaaa tatacccatt
    11521 cgatatagac tgcgaatcga ttttgcaact gcttcagaat caagagaatc accaactcta
    11581 atactaagag ctgaagtaac cagttccgga tcaatatttt ctgctccgat aacttttatt
    11641 tcgtatatgg tttctcctgc agcatacagg ttttgggcaa aacagccaag cagcagaatt
    11701 aatatgctgc atagtataaa tctttgcatt tttcctccac ttaagagtta atagacagct
    11761 ttgtgaacag cgctttttag taaagacatt tttttatgtt tttaaaacaa aagctgtggc
    11821 cttgacagga aagctgtttt tttaaagtag tttcagtatg aagaaatcta tttgtcttat
    11881 tcataaattg ctttattatg taaactgagg ttgtaataat gaaatcagcg aaaaaaatat
    11941 tgattattct aggcttaatt atgtgtttat ctatgctcta cgggcaaaaa actcaggatt
    12001 tcaagcccgg gagaaaatgc agtttccccc aaacatatac aaacacagaa ataagcagag
    12061 ccgattctgc tcacggtttt gatgtgcaaa aatatgaaat cacgctttct attaacgatg
    12121 cttttcacaa tgtatttggt aatgttttag ccacggtgca agctactgaa aacctttcca
    12181 gtatagctta tgaacttaaa ggtttaaatg taactagcgt taaagtaaac ggaattaata
    12241 caaactatac tcattctttg gggttgatta ctattcctct taatgttata gctggagagc
    12301 agtttacaac tcagattttt tattccggaa atcctgtatt atccaatgat gcttatcata
    12361 tagggatgat ttttagctct aatacagtct ttacaatttc cgatccagac gcagcacgct
    12421 attggtggcc ttgttatgat cacccttggg ataaggcaat tgtagattta catattacta
    12481 tgcgcagtga ttggaaagtt gcttctaacg gaataaggac agcaattgta gataatggag
    12541 atggaacttc cacaactcat tggagcggac aaaatccaat gactacttat ctggtttgta
    12601 ttactgcagg tccctatgta gaaatagaac aaacagttcc agaacagaat aatctgccaa
    12661 tccaaaactt tgtgatgcaa aatcaatata acaatgctct aatagacctc caaaatctcc
    12721 ctctgatgat ttcctggttt tctgaacttt tcggggatta tccttttgaa aagtatggaa
    12781 atgctgttgt aagtatgagt acatacggag ctatggaaca tcaaacaatg acaactcttg
    12841 ggaattatat cattacagga accggatact atgaaacagt tattgctcat gaactcgttc
    12901 atcaatggtt tggaaatgct gtaagcttct taactttcaa agatgtttgg ctttcagaag
    12961 gttttgctac ttatagtgaa tttctttgga tagataaaac ctcaggttgg caaagtgctt
    13021 gtgattatat gcgtagtagc atccaacaat attatctgaa ctgggaaatt gcagaaggac
    13081 ctcaaacaat atataatccc tctttcaata actatttttc tccaccttct tatgaaaaag
    13141 cagctagtgt gttacatatg ttaagattaa agctcggaga tgccgatttc tttgctctat
    13201 tacagcaata ttataatacc tataaaaaca gtaatgctat tactgccgaa tttcagaata
    13261 tagcagaaca agttagcggt caaaacttga gctatttttt caaccagtgg atttacggct
    13321 caggcattcc gaacattcaa tattctcttt ggcaaaatga aataagtaac aacctggaaa
    13381 tatatgccaa aactacttct cctacagtta cttcttttac ggtagacatt cctttccgga
    13441 tctctcaaaa tggtattagc gattccttgc tggtaattgc cgatccggat ggttatgcta
    13501 atgattataa ttatccaatt attagcgata atatggaagt tgaggcaaat tataataatt
    13561 ggactttgtt aaatgactta actgaacaaa ttcctcgatt tacagaatgc ctgccttcca
    13621 atgattgtgt tatgctcttc tggaattcat ttatttctga aaaggactat cagtatcagc
    13681 tttaccgcaa actgagtagt gaacaagaat ggactttaat atccacaatg gataacaaca
    13741 ccttcagtta tgctgataca actgtaagcg gcggtttcaa ctatgattat agattgcggg
    13801 tattagatat aaatggctat ttctctaaat tcaccgatac tattactata actccccaat
    13861 catttagttt cgccaatgag ttcttagtgg tagatgaaac caaagatgga aatggcttta
    13921 caattaatcc tgatgatgaa atggttgata gcttttattc tgcagcttta ggtggatttg
    13981 cttgcccgat tgataattgg gattgcgcaa ctcaaggttt acctgatctg gctactttaa
    14041 gtaactataa acttgtactt tggcatgctg acgatttcag ccaaaatctg ctgcaagata
    14101 atctatctgt aattagtagc tatatcgctg gaggtggaaa attagttctt tctgggtgga
    14161 aaacagcttc taatttaacc actgaattct tggaccgttt tgcgggagga gtaactcctg
    14221 tttatgatgc ttctccttgt ttaattagtg ctcattctga tatatataat aatttaacgg
    14281 ttgatcccga taaaaccatt gtttcctgga attcaatgct tccctacatt tatactttca
    14341 acaatgccgc tgaaccaatt tatactgcca atatgataga tggcagtaat ggagcaggac
    14401 aatgtattgc tttcaaacac gattttaacg gcacttttgt aatgtttggt tttcctctgt
    14461 attttatgca gtatccagaa gtaagaatta tgttgcatca gcttctgcca acccttaatc
    14521 ccgctttacc tattacggat gaaacagtgg ctatgcctga agtatctata tctgttatgc
    14581 ccaatccttt taacccttcc acaaccatct ctttttacct tccggttagc ggatatatag
    14641 aatgtgaact atataacctg aaaggacaga aagtgacaac attagcaaag gaaaaaatga
    14701 ctgccggaaa gcatagtatc agttttaacg gttgtgattc acaaggacgc aacctagctt
    14761 caggtgtata ttttctgcgt ttgcaacatc ctaacggtgt aataaataaa aaaataactc
    14821 tcttaaagta ggtactcaaa tgttttattg tccaactcgc atcatctttg ccgatgatgc
    14881 tattcaaaag gcaaaagata agatagccgt tttgggaaaa aaggcattat tggttagcgg
    14941 agaaaaaagc gctaaacaaa gtggagcact atctgatctg ataacagttt taaacgaact
    15001 ggaaatacac tatgtccttt ttgatcgcat taaagaaaat cccaccctgg atagcgtgat
    15061 ggaaggcaag caaatctttt tacaaaataa ttgtgatttt gtaatcggca tcggaggagg
    15121 tagccctatt gatgcagcta aagcaatatc tttggctgcc gcgaattctt tgaaccgcga
    15181 tgagatatat aacattgctg ccttcaagaa tgtatatcct attatggcta taccacttac
    15241 tgcagggaca ggatctgagg caactcaata tagcgtccta tccgatccca cgcataagaa
    15301 aaaagctggc ttgggtagtg atttagcttt tcccgcgttg gctgctatag acccgaaata
    15361 cactttaact ttaacgccaa aagtaactat gcatacggct ttggatgctt taagtcatct
    15421 tttagaaggt ctgtatagca atcagcgttc acctctggtt tatcccttta tttataaagg
    15481 aattgccagt ataatgaaat gcttaccaag ggcattatcc gaaccagata atttagccgc
    15541 aaggaaagaa ttgatgcagt ctgctcttta cggaggaata gttattgctc aaggaagtac
    15601 tactcttcaa cattccattg gttatccttt aacttccaat tacaatattc cacatggttt
    15661 agcaaacgct atggttatgg aagatattat ggaactatat tatcctgccg tttctaaaga
    15721 actaaatgac ttgttcgcat accttaatct tagtaaagat gcattttatg cctggctaag
    15781 agctctgccc tttgaacgaa aaatagagct aagcgatacc ttcatagaag aggcaattcc
    15841 acaagttatg ggtagtagaa atatggctct gaatccatta cccgtttccg aagaacatat
    15901 aagaattatt tttgaacata ttcgaaaaag ttaaattatt ctcagttagg tgtcctttgt
    15961 actttaataa ctaatgaata tattagaact ttcaaggcaa atgggactta ttacggcaat
    16021 tcctaacatc aaaattccgt tgatacccac attaaccact gtaaaatcta caggaagttt
    16081 cgtcatttgg ttgccagggg gacagttagg gttccgttct gtctgtaacc aatgactcct
    16141 aacctgtata tagagaacac ttgtatggct tgttataact tattaaatag ttagttatgc
    16201 gtgatatacc tgttcggtta cccaacgcaa taaataactg ccttctattc ttctattcgt
    16261 aagttattca taaataaaga tatagtatca ttattcagtt ttggaacaca tcttgcgatc
    16321 tattcttgac aattttacca aagttttgga aaagagttat gtgttcatct attgcaaaat
    16381 gccatacgca gaaaaaaatg aataaataac ataactcgag gaataaatgg aagaaaagga
    16441 aattaagctt ttagaaaaag cattatccgg caagccgaca aaagataatt tttaccaagg
    16501 gcttaaatta gtaagttact atatcggttg caatccccat aaggcagagg aatctaccca
    16561 aatattattg caaatggcta atcttatggg aaccgctgaa gaaattgcct ttgccaataa
    16621 aacgatggga tcggtaaatt ccgttttagg caaccatcag gaggcaatcc cttattatca
    16681 gaaagcggta aatttatttt ctcaaatagg caaaaatctg caaatggcac aatcgcttgc
    16741 ctctattgct tctatgaata ccactttggg aaacactgcc caggcattga agacctttat
    16801 ggaagtaatc cctctttttg aacaggaaaa cgaccagcat agtattgcca taacctattc
    16861 taatatggca aatatctata aagctaaaat ggattatgcc caagcaattc agtattatca
    16921 aaaggcagca gatcttgcgg aaagcattca atgtctggat ttgcaagtta gttctctttg
    16981 cataatgggt tccatctatt catctttaat ggattacaat aaaaaccttg cctgctgtct
    17041 gaaggcatta actttggcag aatccaccgg caataattat ctgataggcg aggtccattg
    17101 ctctttaggg gttgcttatc aggagttgca ggattatgat aaagcattgg aatattatta
    17161 taaatcgctt aaaattggaa gggaacagca gattccctat tatatttcag gtaatttaag
    17221 taatattgcc gatatttatc gtaagcaagg aaagctgaaa gaagccctaa aagcatataa
    17281 agaagcatta aagtatgatt tgcaaaccgg agatatttat aatcagagcg cttctttacg
    17341 aactatagcc cttacttata tagatatgca tcgttacgca cttgccagga aaaccatttt
    17401 acaagcatta aacataattt ctgctgatga acatctggag atggggataa atctgcaggc
    17461 agaccttctt ttaacttgca tcaaacaaca ggactataaa tatgccgaag ccctattaaa
    17521 aaaattgcta aaaaaagtgc aagacaaccc cgtttccgaa gtgcaaattt ccatctatga
    17581 atatgctgcg ggactctata caaagttacg aaattataag aaggcaaatt actttttaaa
    17641 tctccattca caactctaca aggaatttat caatcaggaa cgggagaaaa ttaaccgctt
    17701 tatgcagatt agttttgaaa cagctcgcct tcaggatgaa aaagaaatgc ttcagtttca
    17761 gaatgaactt ttggaaaagc gcgtccaagc agagctgaat aaaataaaag aaaaggacaa
    17821 attactggct caacaagaac aattggctat gttgggtaaa atttctgcag gcatcgcaca
    17881 cgaaattaac aatcccttgg ctgctataaa acaaattgta gaaattactt tggaggactt
    17941 aaatagtttg cagccaaggg gttatctttc acaaaagaaa aatgatgtgc tacaattatt
    18001 ggagcgtatc aatcgcttgg tggaaatcat taaagttatt gccagaagtc ccgaaaggtt
    18061 caacgccaat tcttttagtt tgaatcaaat tatagaagag tttatcgaac tttttggcga
    18121 taaaataatc agcaaggatg ttttgctgat caaggactgc gaaccagatt tacctcctgt
    18181 ttatgcagat aacatcaggt ttattcagct tttatccatt ctggcaacca attccaatga
    18241 tgctcttaag aatgttaatc gctccgaggg aaaatatatc acaatttcaa ccaagttaat
    18301 agatggcagg ataatgctgg tagtagaaga taacggttgt ggaatggatc aagagacctt
    18361 aagcagagcc accgagccat ttttcagctc caaagaaatt ggccagggaa cgggattggg
    18421 tttatccttg gcacatagca tcgttttaaa cagcaaaggt aatatgacaa tggaaagtga
    18481 actgggaaaa ggaacctctg tttcactcag ttttcctgta gaggatcatt atgaaaagca
    18541 atgatttaac ccatatactt gttttggatg atgaaaccag tatccgcgat ggcttgatcc
    18601 attatttcag cgaacgaaat tatcgcatca gcggagcttc caattgggaa gaagcacagc
    18661 ttgttctaac caaatctatc gtacatatca taatattgga tgtggtttta cccggttttg
    18721 atcgtaaaaa agatggcttg gatgttctgt cacttattaa acaaaaatat ccggatactg
    18781 aagtgataat gatttcagga cacgccaccg tagaaaaagt aattaccgcc tggaatcagg
    18841 gtgccctgat gtttctggaa aagccctatt cacttgaaga aatggaaaaa gcggttaaac
    18901 gcgccgaata ttacagccgc ggtttttcgc gtttgcatca attggaagca caagttatca
    18961 gccaaaaaat ggagcaaagc ttagggacta aagttattgc caaaagcccc caaatgaaaa
    19021 aatgcctatc tcttgccaaa aaatacgccc aaaataacca ttctgtttta atcaccggcg
    19081 aatcgggaac cggaaaagaa attgtagcta agcttattca ttatttcagc aaccgttctg
    19141 aagaagaatt tgttgccgtt aatgtaaatg ccattcctca cgaacttttt gaaagcacct
    19201 tatttggcta tgttaaaggt gcctttacag gtgcaaacac caatactaca gggctggtgg
    19261 aaaaagcagg taagggaact atttttctgg atgaaatcgg tgacttgcca atggaacagc
    19321 aggtaaaact tttacgctta atagaaacca gagagtatta taaagtgggt tccagccgac
    19381 cttcccgaac cgaagcccgt tttatttttg tcacaatgca agacctgaat caaaaagttg
    19441 aacaacagtc ctttcggaat gacctatttt accgcatttc cgatttggaa ataaatattc
    19501 ctcccttgcg ggaaagaaaa gaggatattt ctgctttgtg cgaatatatt atcccgcaat
    19561 tggcaaaaga agaaggtatc gccaagatac ctgttttgac taaagatgcc tataaggcca
    19621 ttcttactta ttcttttcca ggaaatatcc gtgagctgaa aaaaatcctg cgaaaagcaa
    19681 ttttgaacct ggaaagcaat gaaatactca aagaagacct tttcccgcca gttgaacaaa
    19741 atgttaccaa aactaaagga actttttgtc ccgattgtga ggcatatttc atcaaaaaag
    19801 ttcttactaa tacagatttt aacgccagct taacaatgaa actcttgggc atcagccgca
    19861 gcagttttta cgataaatta aaacgctata acatcaatgt taaacataat atcgaagagt
    19921 aggaatatga tctgagggta tcccttgtat gtgctgaagg atatcaaaca aataggtgga
    19981 tagcttgttg ctatccacta tttctttaat aacagatctt tacgcaatat ctgccctcac
    20041 ttaattgcct tatcattatt attgccttat cattattatt gccttatcat tattatttat
    20101 gccaatagag gtatttgtgc gctatacagc tcgggatata attactgaaa accaaaacgg
    20161 aaagctaagt ttagctttcc gttgaaataa gaggagagat tgagttaatt tactttatat
    20221 tttacatcgc ctttttcgaa ataagcgata atttgtttag ctgcagcaat acctgcattt
    20281 gtattagctt cgctggtttg ggcacccatt tttttaggtg taaagaacac ccggtctgga
    20341 tagagttcta ataatttagc actattatcg ggagcgacat cactcacata acggaatttt
    20401 ttcttttcgg caaaagcttt aagcaaagca tcttcttcaa taacttcttt gcgggcagta
    20461 tttaccaaaa tggcatcatc cttcactaaa gataggaggt tccagtttac actcttttta
    20521 gtgtcttccg ttgcgggaat atgtaatgat atataatccc ccagtttgaa tatgtcttct
    20581 acgctatgca agggtttaac accttctttt tgaattactt catcggaaat aaaaggatca
    20641 taggcataaa catccattcc gattccttta gcaattttgg caacataacg agctacccat
    20701 ccaaaaccat atagagttag tgttttacct gcaagttcag ttccagattt tccattgaac
    20761 ttattccgtg ccatataaat catcattccg attgccagtt cagcaacggc attagaattt
    20821 tggcctggag tattcataac aacaacattg tgagctgtag cagatttcag atcgatattg
    20881 tcataaccgg ctccggcacg aaccacgatt tttagttttg gtgcagcatt aaaagctgct
    20941 tcatcgataa tatcgctacg aacaattaaa gcttctgcat cagctaaagc attgtgcagt
    21001 gcactctttt cggtataatt ctccaaaaag gagtaattat aaccagcatt ttcgagttca
    21061 gttttaattt tctgaactgc atctgcagcg aagggttttt cggttgcaat aaggactttt
    21121 ggcatttttg cctcctatta ttttgtttgt ttacttgctt attcatttcc ctaaaattcc
    21181 taaaaggaca ccagcagcaa ctgctgaacc aacaacaccg gcaacatttg gtgccattgc
    21241 atgcattaat aaataattgg ttttatcata ttgttgacca acaacttgtg aaactcgtgc
    21301 tgaagctgga actgcagaaa cacccgcact accaattaaa ggattaatct tatttttgga
    21361 gaagagattc ataattttgg caaaaagaac acctgcagcg gtagccgctg caaaagaaaa
    21421 tgcacccagg aaaaagattt tcaaagtggc tggagtaaga aatacttctg cagtagtttt
    21481 tgctcctact gttaaaccaa tcaaaacggt tacgacatcg attaaaacag tcctggcact
    21541 attggcaagg cgttcagtta ccccgctttc ttttaacaaa tttcccaaaa agagcattgc
    21601 taaaagaaca acacttcctg gagaaataaa agttgtaact acgaaagcaa cgataggaaa
    21661 gatgatcttt tctttcttag aaactactcg taagggtttc atccgaataa gacgctcttt
    21721 tttagtggtt agcaatttca taataggtgg ttgaataaca ggcaccaatg ccatatatga
    21781 atatgctgct aaagctattg aacccaaaag atgaggtgcc aacttagaag ccaaaaaaat
    21841 tgaggttgga ccatcggcgc ctccaataat tcctattgaa gctgcttcca tcacattaaa
    21901 tcctaaaagg agactgccaa taaaggtagc aaaaattcca aattgagcag cagctcctaa
    21961 aaggataagc cgcggattgg caattagagt ggaaaaatca gtcatcgctc ctattcctaa
    22021 aaagataagc gaaggataga tacttttatt cactccaaaa taaagatagt ttaaaacaga
    22081 gccctcattc ataactccca atccctgttg agaaacacca ggcatattac caactataat
    22141 tccaaaaccg ataggaatca gtagcagtgg ctcaaattct tttgcaacac ctaaatagat
    22201 aaagatcatt ccggagataa tcatcaccag atttccccag gtaacattca ggaatcctgt
    22261 ggtttgtata aattggctta attcgtgcat aaagctaatc ctcaaactca attaaaggat
    22321 cattatcctg aaccaggtct cccttatgaa cataaacttt acttactttt ccggttcgag
    22381 gactgtaaat atccgattcc attttcattg cttccagaat taaaacaata tctccctctt
    22441 tcacatagtc attaacctgc acttttatat ccataatcgt tccaggaaga ggtgcgcgta
    22501 gaattttatc tttatttcca gttttctcgc tttctttttc aggactgtgt ttaggagaag
    22561 tacttatagg aggagatttt atgtctaccc cagtaatttt catcagcaaa tctccttctt
    22621 taacaggagc tctttctttc actagtatct tctcgatttt tccatcaaag ggagaggcaa
    22681 tttcgctttg cattttcatc gcttcaatta ttaagatcga ttgtcctgct tttacttcat
    22741 caccttcgga aacagggata gtaactatta ccccaggaag gggagcgcga atttctccag
    22801 aaaaaagatc tgcattacta ctaaatgcag gagctaatgg aacagctttt tcctgcgcag
    22861 caagctttgg tacactatca attttatcat tttcaatttg aaccagatat tctgtaccgt
    22921 ttaagtttat cctggcatgc gtaggactat attccaagat tttggcttcg aagtgttcgt
    22981 tattgattag tagtttatag gtcttcatct atatctccgt ctattattca tttctctgtt
    23041 tggcatattc aacatagcat ttgcacgcca ttgatttgta ggagtgcgtt tgaaggttag
    23101 ggcaattttt ctttgatctt ccagagtttg ttcatgtaac atcacagctg taactaaagc
    23161 agcaattaca ttctgattta aagtggcaga agcagcacga acttttcccc cgggtgatat
    23221 tttaagatcg gctttttcct ttttgggagg acgattcaaa aggttaagct gtcccactat
    23281 aatactggta attaataaag ctgtaaaaac tgtcagcatc cctaccagag ttacattaag
    23341 cccataattc atactctgtt cattgaattc ctgttcaatt tttataatgt cttctggagt
    23401 tacattcaat gattgtaatg agtatgaatc ccattgctga gaattgggtt tttccaatcc
    23461 aatcttttgc ttaatttttt ttatgggcat attcagaact gaggcaactt cgtccagagt
    23521 tgaaagttct gtaaagccat aaacactata ttgttttgct aagagagctt tataaggagt
    23581 gatacccaat ttgcgtaaac tcatcttatc caatttttcg ttataaggtt ccaaacccag
    23641 atattttttc caactggcga tatctttaat ttgtaatttt tctgctactt ccgcaagggt
    23701 agcagtatcg cgaaagccat attccgacaa aatttctttt tcgcggacaa gagctaagct
    23761 atcagaaatt gtttgctcta taagatctat ttgagcaaat gcactcaaaa agctacaggc
    23821 aaaaatggct aaaaacacta tctttcgcat tgcctatacc ttacagaggg atattgccat
    23881 gtttgcgagg aggaatgcta tcctgtttat tagccagcat ttccaaagcg cgaattaaac
    23941 gaaatcttgt cgcagagggt tcaattatat catcaatata tcccctttct gcagccacaa
    24001 agggattggc aaatttttcc cgatattctt tttctcgtgc ctgaagaatg gctttaggat
    24061 cctctgaagc cattgcttct ttacggaaaa ttacttctac agctccctcc ggacccataa
    24121 ctgcaatttc tgcagatggc caggcataaa caatatctgc acgcatatga cgactattca
    24181 ttacacaata tgcacctccg taagctttac ggataataac tgtaactttg ggaactgtag
    24241 cttccgcaaa ggcataaaga agttttgccc cattacgaat aattccatta tgctcttgca
    24301 tcgttccagg taaaaaacca ggaacatctt ccaaaaccac cagaggaata ttgaaagaat
    24361 cacaaaaacg cacaaatctg gctcctttga tactggcatc aatatctaaa acaccagcta
    24421 aaatctttgg ctgatttgca acaataccta ctgtgcgtcc attcattctt ccaaaaccaa
    24481 caatgatatt ttgagcaaaa ttactcatca cttgcaaaaa ttcaccatta tccacaatcg
    24541 gatatatcac ttccagcata tcatatggct tatttggatt ttcaggtacg agattattta
    24601 aaacatcaca attacgagtg ataggatcag cattgggaac atagggagga tcttccaaat
    24661 tatttgaagg caaataactt agcagcaact tgatagtaag catagcatct tcttcgctat
    24721 tggcaataaa atgagctaca ccacttttac tggtatgcac tttggctccg cctaaatctt
    24781 cattagtaac cgtttcattt aaaacagttt taactacctt tggcccagtt acaaacatat
    24841 aactgttgct tttttccatc acaacaaaat ctgtgatagc aggagaataa actgcaccac
    24901 ctgcacaggg acctaaaatt gccgaaattt gaggaattac tccacttgcc atcacattcc
    24961 gccaaaatat ttcagcataa ccggcaagag catcaacgcc ttcttgaatt cttgctccac
    25021 cagaatcatt taaacctata atcggacaac cattcttcaa tgccatatcc attactttac
    25081 agattttatc tgcgtaagtt ttagagaggg atccaccaaa aacagtgaaa tcttgtgcaa
    25141 aaatatagac caatctctca ttgattgtag cgcaaccagt tactacacca tctccaggat
    25201 gtttatatcg ttccatccca aaattggtgc aacgatgagt tacgaacata tcaaattctt
    25261 caaaactgtc aggatcaacc aagagggcaa tcctttccct cgctgtaagc tttcctttta
    25321 gatgttgttc aacaatgcgt ttttctccac cacccaaaag agctgcagct cttttagctc
    25381 ttagcttttc cagaggattc tggttttcca tttctttcct ttcctattct atgtttattt
    25441 ttttgtatta agttatgcca tagtcattat tttaaaagct gttttttagt aaagggaaat
    25501 gttctgggtt tttttatagt tgtgatatct aaagctgtct tctattgttt tcctggcaaa
    25561 accaaaatat tcatattatt atagcaatga ttgctacaca tatctttcaa aatgaattgt
    25621 actgcaaatg cagattacat tgcataatgt gtacaccgat ccaattggct tgaccgtttt
    25681 ttctttttgc caacttcttg catactaaca cttccaatat atagcagatt gtttatctat
    25741 gcattaagta tattataatc gataatccga agctcttgag attcactaac accaggaata
    25801 ttaacaacat ttatagatcg tttcaaccta aaccttacat ttcttgcaag ttaccgatat
    25861 tcaacttgtc ctccacctaa tcttatctca tccttaacga tgagttgaag aggagaagag
    25921 gaggagagga tgatgggttg tttgcttgag ccatcctgaa atcagtggac taaaattaag
    25981 gagaaagaaa tgaataaaaa ttctacaaaa aggatgcgag ttcgctacag cgatgccttt
    26041 gaaccttaag ctgctcatat atgaataacc tgagtaatct gcataatctg cataatctgc
    26101 gtaacttgcg tgagttattt ccggtggctt tggtggctaa atactcttct aatattcttg
    26161 cttataaagt taatatctct taatcctata atccttaaat ccttgtttct aacatttaaa
    26221 cccttaaatc cttgtttcta agactaaatc tttgggtact aatcctaaaa tcgcagacct
    26281 tttctcttag ccctaagctc tctacactct gcaaatataa tcccgttcat ccttggtata
    26341 tttcattttt gcccgttcag acaagcatct atgcaaacaa taatatcttc ctgctaagaa
    26401 ctaactcatc tgaatacaag gttatataat agaccccggt ttcacaaagc tgtccatatt
    26461 tatctcttcc atcccagatc agagaattat cagtaatatc aaaactgatg acttcctgct
    26521 ccaacacatt ggtaattacc agtttataca atcctgcaag gtgaaagtta aagttcacaa
    26581 caacagtttc ccctttcact acaggatttg gataaacttc cattaagcaa ttatcattgg
    26641 cagtagattc atcagcagtg gattcgggtt ttgtagccaa actgcatccc ataagcatta
    26701 taagcagaaa aatgcactcg gcaagaatat atattttagt catattcatc ttagttcctc
    26761 acctataatt tatttaagca tttctgaagc aatctgaaaa acatttaaat tgcaaccaaa
    26821 actgtatatg ttcttatctt tgcattggct tcctccgggg aaataagaat agtttccatt
    26881 aagtgaaacc agatttcgat aatgcaaatc caaaccaatg ccataagtaa gaccttcaat
    26941 atttccccct ttatctttat gatagccact tcttaaaaaa atggtatcca gaaaaccaag
    27001 ctctgttcca attccataaa tattggggct ttgtgttcga tcatcattca ttccggcaag
    27061 aagacgcagg catccaatat tttcaaaatt atccgttaac tcattctctt tcagatagtt
    27121 cggattttta agcgaggcag aacaggcaat tccagtgttt atgcgtttgt atattttatg
    27181 ttgctgacca ctattatatt taataccgtc cccataggca ttgaacatag aaaatccggc
    27241 tacagattcc agatgtattt tgtcggttaa gggaaaacta tattttaaca gagctccgga
    27301 atctacacaa tcagcatcat attcttcacc tatattagcg ggataactat agccaatatc
    27361 aatagaactg caatttagaa tataattcat accgaggcag aggtctaatt tcttgttcga
    27421 gaatgtatta tcgttatgaa taatccgata gagatcggca aaattaactg aaattcccaa
    27481 gggatacata aattccttaa gttgcttcgt aattcctatc tcataaccat tttcatcgac
    27541 aaatgctatg cttccataat cagcatttat tccccaagta tgaacattgg taacagaagg
    27601 aaaaagaaag ccaaaaccct tataagataa actcatcatt gaagcataat aggcgctttc
    27661 tttttcatct ccgggatgtt tcaatctcat aaaatcatag caagtattag aataactgag
    27721 ccctgtatgc aaagatggat aagcaggatt gtcataagca tttaaaggtt cattgtgcca
    27781 aatatttaca actcctacag gacttccacc caaggcaaga ttaattgccg agggctcaaa
    27841 acaacttacc attaaaacat aattggagat agccgctaaa tttgctatca ccacacctaa
    27901 tataaaagcc agcaagatta tttttttcat aaaaaactcc tcaaaacctt ctttttttac
    27961 ttgtaaaata ttatcttttt ctgtca
//
LOCUS       DAOSTH010000015        27059 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_1969_length_27059_cov_13.229003, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000015 DAOSTH010000000
VERSION     DAOSTH010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 27059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27059
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1969_length_27059_cov_13.229003"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="PLB65_03710"
     CDS             <1..653
                     /locus_tag="PLB65_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ77664.1"
                     /translation="FDPQGNATSGVGIDKDKVELQIYDALIDRAPIEKTILATSTENL
                     YCIPGNINLTGAEIELVQEYAREHKLKNALQPIVNSFEYIIIDCPPSLGLLTVNAMTA
                     ATEVLVPIQCEYYALEGVSQLLTTIRLIQKNLNPGLNILGVLLTMFDKRVNLSLQVAK
                     EVHRYFKEKVFRTIIPRNVKLTEAPGFGKPIFLYDIRSPGAMSYLNLANEVINRSK"
     gene            664..1518
                     /locus_tag="PLB65_03715"
     CDS             664..1518
                     /locus_tag="PLB65_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOQ77665.1"
                     /translation="MNDRLGRGLSALIPENDLEENPKLGISTLPIDSIKPNRYQPRKI
                     FDAEKLAELTESIKENGIIQPLIVTKTTSSEYELIAGERRLEAAKLAGLEKVPVVIRS
                     VSKKEQLQFALIENIQREDLNPIEEALAYNALVEDFGLTHNQISEIVGKDRATITNSL
                     RLLKLPERIKQMLISGELNSGQARAVLSVKPELQISFAEYIIKYNLTVREAEDKAKTY
                     SEDKTAAPQKAKTSSRVVDLEKELHQLFKMKVKVQEANGKGKITFVYKSEEELLKFKT
                     ILDKIRQE"
     gene            1518..2588
                     /gene="rfbB"
                     /locus_tag="PLB65_03720"
     CDS             1518..2588
                     /gene="rfbB"
                     /locus_tag="PLB65_03720"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOQ77666.1"
                     /translation="MKSVIVTGGAGFIGANYLHTLFADENFSGKILNIDKLTYAGNLD
                     SLQDLESKYGNSRYFFVRADICDAEKMKEVINQFKPDTIVHFAAESHVDRSIDGPMEF
                     VKTNLVGTATLLNAALEYYRTLQEAEKKNFRFHHISTDEVFGSLGESGMFTEETAYDP
                     SSPYSASKAGSDHLVRAWQRTFGLPITISNCSNNYGPYQFPEKLIPLMILNCLAHKPL
                     PVYGKGLNVRDWLFVTDHCEAINIIIRKGKFGETYNIGGHNEMKNIDIVTTICKILDE
                     LEPSPTLNSYTELITYVKDRPGHDLRYAIDAGKIEKDLRWKPAETFETGIRKTISWYL
                     ENREWWQNIQNNNYRQERLGLG"
     gene            2684..3808
                     /locus_tag="PLB65_03725"
     CDS             2684..3808
                     /locus_tag="PLB65_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOQ77667.1"
                     /translation="MKKDVVNYFLELIAIDSESKNERAVIDKLKLDLEQLGAIVEEDD
                     CYTKTNGNAGNLYAYFPGTINKKPLLLCAHSDTVTPGKAIKPTIASGRIFSDGTTILG
                     ADDKSGISEIMVAIKNIKDKGIEHAPIEVLITVSEEIGLLGAKCFDKSKLQSKIGYAL
                     DSHQVGELTIGAPYQNTFCITFYGKEAHAGVEPEKGLNAIRLAAESIVAMPNGRIDFE
                     TTCNLGAIYSGSPTNIVPKEVVVKGEARSHKKAKLDQVCDEIKQAVISTVKRYNDQNG
                     NASYKFELEQEYEAFFIPNNAPVVSLAENALRALDIPSNIKIGGGGSDANIFNAGGIQ
                     MIIVGTGMNNVHTVNEDITVDQLYKGSAFVEQLIIQYAEN"
     gene            3809..4558
                     /gene="dapB"
                     /locus_tag="PLB65_03730"
     CDS             3809..4558
                     /gene="dapB"
                     /locus_tag="PLB65_03730"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOQ77668.1"
                     /translation="MGLKLCLIGYGKMGKMLANLATNYDCTVVSIIDPQQNNYHSEIS
                     LQSIGEAEVCIDFSHPSVVLDNIQKVLSYKKNMVVGTTGWFDHLEEVQKMVENSASGL
                     IYGANFSIGMNIFNRLVAEATKAFDRFPDYDVYGLEMHHNQKADSPSGTAIELSKTIL
                     ANSSHKKTTLFDPAYRKIEPSELHFVSLRAGSIPGTHKVGFDSEADTIELSHCVRSRS
                     ALASGALQAAKWIKDRKGFYSFSDMVSSVLC"
     gene            4552..5340
                     /gene="dapF"
                     /locus_tag="PLB65_03735"
     CDS             4552..5340
                     /gene="dapF"
                     /locus_tag="PLB65_03735"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOQ77669.1"
                     /translation="MLIPFIKMNAQGNDFVILDYFAPELVTLKELNYSSLALEVCRPH
                     FGIGADGLVLLKPSTEFDAQMIIFNADGSQAEMCGSALRCVTWLLYKKTEKREFEILT
                     DSGVKKCQVNNDKTITANLGLPKMLKRKYNAEGFTGDLIEIGNPHFIVWQNNSDDDPH
                     IKYGPLLEHHQGFPDTVNVHFAYLINRNEIAIKIWEKGCGATFACGTGASATVYSGII
                     NGFLDNEVKVNMPGGTVKISATAQGYLLSGEVYETFQGVFKWRI"
     gene            5328..5948
                     /locus_tag="PLB65_03740"
     CDS             5328..5948
                     /locus_tag="PLB65_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ77670.1"
                     /translation="MENLGKYLLELRLQRGYSYQDIWNELRFKEDQIKAIEENRLSAL
                     GPYGIAKAVVFNYARYLEADLNSVMNAFSIMMPESNKPEFKPATTVKEKKIMLSTNFM
                     WTLGILVIIIILGSILWYAYNQNWLRPPNFFTAKKSDSTAVSIKEQKEETKPDTLRQH
                     LQAISEAIPKTNNIKIDNLDSKTVPPDTTDYLGNILGDSPINVPIH"
     gene            6180..6617
                     /locus_tag="PLB65_03745"
     CDS             6180..6617
                     /locus_tag="PLB65_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOQ77671.1"
                     /translation="MKLVPYRRTNELRPMSSMLNLFDDFFNRFYEEEVGDDNFQPMAM
                     DIIEQDKEFQVLANLPGFKKDDVKISIHDNQLIIEANCNTTKEETKGTVYRCERYSGS
                     YRRNLVLPENAEISKISAKMEDGVLKLTIPKKEPSPKKEISIQ"
     gene            6766..>6952
                     /locus_tag="PLB65_03750"
     CDS             6766..>6952
                     /locus_tag="PLB65_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77672.1"
                     /translation="MKTSIFLFFSRDYATIAMIVLVFELKSVLLVICHTISFFLLSLN
                     KVINYLIENADFDLKPTG"
     assembly_gap    6953..7052
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7098..10097)
                     /locus_tag="PLB65_03755"
     CDS             complement(7098..10097)
                     /locus_tag="PLB65_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOQ77673.1"
                     /translation="MKHFALLLCLLMTVSLFAYDFNDREGIAVFKIKPEMRSALQKNN
                     NRTGIATIDEKLQKLQVSSIHPKFNLSPQKRNNCELELIFTVQSPYPPQTIVNLLATD
                     NYVQYAEIVYPDSIFAIPNDENYAESLYFSSLEAESAWDIHKGENGSNSVLLAIVDTG
                     CRWTHPDLVDNIWQNLGEDVNDNGYTIYYNGSTWVMDAGDLNGIDDDGNGKIDDLIGW
                     DFMLTSSGEEANNPFESSGHGTTVCGIASARTNNIIGVSSLAWNLKMLPISCSFPGSS
                     SIYRGYDGIIYAAEMGADIINCSWGGSSFSWANQEAINYAYSLGSTIVASAGNNNNAI
                     PVYPAAYQNVIAVAALQNNGIKSSVSSYGSFVDVGAPTESIGTVTASGYTTVSNATSY
                     ASPVASSLAGLIQSYLPEISQTELRDRIKGSCDDVDAVNLGRENLLGEGKLNARRALQ
                     ESNPLPDVEIRLALIENRGATDANGNLAVEPDETFSVNLLLRSYGEYASNGNFTLSTG
                     NSYVNILSGSHSQTIPADGYLLLENVFSIYVLPSATSQYVTFTLNTVADYPVVAGANL
                     SFSILINAGGIFVWEGVAGGRDMSGTFINNTLQDLGYDCVYGTTFPSSFYSFEAVFLS
                     FGAVGSNIVRFDNLLMYNALYSYLISGGKVYIEGVDVVGFDFGYYLPDVQGEQDADEV
                     LLPLLGIMNAEDGQTNAINNLSGVSNTPTSGMNFTGSTQTKVDYIDRFTALYPYARSA
                     FEESDYGCVAVASAGGYNERSFVFSYALSELTDGTFPNTRANLLKCIMDFFTAEEVTL
                     PVELTAFYAVYANGANIFWNTASETNLAGYNLYRNDRPDFSSALKLNASIISAIGGAS
                     GYHYSFYDSELPLADTLYYWLETIAYNGINNVLGPIVLILPNPEPENPPLPPDKIEYL
                     HSYPNPFSSSVVLELKLSSATESKIYIYNIKGQLIRKMETIPLDTGLQHFAWDGLDNK
                     NRAVPSGIYLIIVKSEQRTIIHKLLKM"
     gene            complement(10107..10460)
                     /locus_tag="PLB65_03760"
     CDS             complement(10107..10460)
                     /locus_tag="PLB65_03760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HOQ77674.1"
                     /translation="MACEFCNLDCSIYLAENEHFFAIWDKYPVSKGHTLIIAKRHTPD
                     FFALTEAEVVSLQSITITVQKLLAEKYNPQGYNLAMNCGSAAGQSIPHFHLHIIPRFY
                     KDGKNIFKRSRESVF"
     gene            complement(10724..11641)
                     /locus_tag="PLB65_03765"
     CDS             complement(10724..11641)
                     /locus_tag="PLB65_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOQ77675.1"
                     /translation="MIVEALDIGSNSIKCLIADKREKSFNCLTDIRIPFRLGSYLTKK
                     GYISELGQNKAIYVLNCLQLDIERAYKVEEIIAAGTETFRRASNRKEFVRRIYIQDCL
                     EVKILTPKQEAKLEWKGVLSGLPNPLQPITVFDSGGFSTEIISGRGGHIQSSQSFPLG
                     ALQLTKQFVKSDPISTKDYNNLLFYINGVLPVSIVTKDTLIGTGGGALVCACVASKGK
                     IKTPQQAEGYEITLAELQRQIELYKQTDLKQRENIAGMEKERADIMLAAVLLFSALLK
                     KSGKDRFRVSTRGLRHGMLTMPKWYVCPI"
     gene            complement(11713..13005)
                     /gene="glyA"
                     /locus_tag="PLB65_03770"
     CDS             complement(11713..13005)
                     /gene="glyA"
                     /locus_tag="PLB65_03770"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOQ77676.1"
                     /translation="MIHIQKTDPEIYAAIMNELNRERENLELIASENFTSLAVLEAQG
                     CIMTNKYAEGYPYRWSKKTGAINYKLYGRYYGGCEFINDAERLAIERAKQIFGAEHAN
                     VQPHSGSQANMAAYFTLVKPGDTVMALELAHGGHLTHGHPLSFSGQLYNIVPYTVNKE
                     TEQLNYDELEALAIEHKPQMILAGASAYPRKLDFARFREIADKVGAKLMVDMAHIAGL
                     VAAGLHQSPVPYADVVTTTTHKTLRGPRAGLILCKEEFAKEIDSKVFPGVQGGPLMHI
                     IAAKAVAFLEALQPEFKLYQQKVVENANALAKALIDQGFKLVSGGTDTHLMLIDLGPE
                     IEGSPSGKKMEEALDLAGITANKNTVPFDTRSPFVASGIRLGTPAVTTRGMGKAEMEL
                     IAELIKKVRDNFENEEYLQALKKEVHSLTDRFPLYPEL"
     gene            complement(13005..13436)
                     /gene="rpiB"
                     /locus_tag="PLB65_03775"
     CDS             complement(13005..13436)
                     /gene="rpiB"
                     /locus_tag="PLB65_03775"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HOQ77677.1"
                     /translation="MKIAIASDHAGYEMKEAIINAFPEIEFEDFGTHSTQSMDYPDTG
                     YPAARAVATKHCEKGILICGSGIGMSITANKVSGIRAALCVNTDIARLSRKHNDSNIL
                     VLAGRFTAIPYAIEITKVWLNTSFEGGRHLKRINKIHQGEK"
     gene            complement(13433..14341)
                     /locus_tag="PLB65_03780"
     CDS             complement(13433..14341)
                     /locus_tag="PLB65_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOQ77678.1"
                     /translation="MITKLTELAEKAINSGKKTRIAVAVAEDNNTINAILKATNNGFV
                     SPILIGNKDKITALLPADIDSNKLQIMNITDPAMAVKEAVRMVRNNEADVLMKGLVGT
                     ETFLKAVLDKEKGLLPPKAVMSYTCALEIPKCHKLLFVSDTAVLINPDLNQKIAMINY
                     SVAMARKFGIEKPKVALISATEKVSSSMPETLEYSLLCKMAERGQIKNCLVDGPLDIF
                     LACDPTSLSIKGIQSSLEGDADILIFPNLESANSFYKGLMLFGEGELAGLICGTTKPV
                     IVMSRSESENSKYYCIALSCLMAEGK"
     gene            complement(14347..15249)
                     /locus_tag="PLB65_03785"
     CDS             complement(14347..15249)
                     /locus_tag="PLB65_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOQ77679.1"
                     /translation="MQISKLDQMFDVLATRPKKRMIAAWGVDSHTILAAYMAVEKELI
                     EATLVGDEKKILSVCKEHNLDCSKFNIVHCPVDIKSASLAVEMINKGKGDFLMKGLIS
                     TDRYMKAILNKECGLMDPGAILSHVTVTESKHYHKLLIVGDVAIIPLPDLNQKIAITN
                     YLIRTANLLGIENPKVGLLSASEQTLPKITSSADAAIIAKMAQRGQIKGAIVEGPLGL
                     DMILDRESALIKGVDSPVCGDADCILFPNIESGNTFYKTIIKLAKSELGAIVMGARVP
                     CVLTSRGDSECSKLYSIALAAMLA"
     gene            complement(15262..15873)
                     /locus_tag="PLB65_03790"
     CDS             complement(15262..15873)
                     /locus_tag="PLB65_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77680.1"
                     /translation="MKMHFIQRLIICAVSLLCFACTSFQKNKSEEEAYKIKASKDSID
                     EYIIPEIETEDKIYVSESVSSTEIIEEQRYVYTVIENVQALLEGDWLSKFQQERRPFI
                     LLISSNIESEYAEQESSLLELIKDELLRSYAVNLVLCDTSLILSSHPDSLLYVREHSG
                     ADFLLQSRYLIENGIPFLELKLYNLQEMACVYDETKSLIKIKI"
     gene            16204..>17634
                     /locus_tag="PLB65_03795"
     CDS             16204..>17634
                     /locus_tag="PLB65_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77681.1"
                     /translation="MKKTLFVMLLALAFLGSLSAQINIQSGQTLTQDFDVLGTSATAT
                     MPASWKVDKNTTVRLVGNYSAAVTATERTGGNNMSTTAGNGIYNYGAGPADTATDRAV
                     GFLSSGSATKSGNVYVQLSNNGSTTINSFAISYNVEKYRMGTNAAGFSIQMYYSTDGI
                     TWTSAGSSFLTSFPADATTNGYASAPGATIPVNATLSYSLSAGSSLYLAWNYSVTSGT
                     YTSYAQALGIDDVSITAGGTASPTIVVSGTLTPFSTYTGTPSTAQSYTLSGQNLTGSI
                     TVAAPTGFALSTDNTTFSSTLTLASNFNGLVYVRLTGITAGTFSGNIVHSSSGATTIN
                     LAVEGTVTNPSPLIDITANLSDFSTILGTPSAAQSYTVTGHFLTSAISITAPTGFNLS
                     TDNTTFTSTLSLASDFSGTIYVRLTGVSAGTFTGNIVHSSTGAPIQNLAVNGTVTDPS
                     VHNTFLDEEFIYPAGDLLTAHGWNAHS"
     assembly_gap    17635..17734
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17735..20440
                     /locus_tag="PLB65_03800"
     CDS             <17735..20440
                     /locus_tag="PLB65_03800"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HOQ77682.1"
                     /translation="KTFANQTSGTFYAAFLFNASEAKTTADYFFHFGPNPIGSDLKGR
                     VFAQKDASDNLRFGITKAAGAISETAATPYSYALNTTYLIVIKYVIVSGAANDEVYMW
                     VNPVIGATEPAAQLTAADLSGTDIAGIGAIAIRQGANTPIARIDGIRVTNDWATLWEG
                     TPMETPVIHTSTPELDPLASIVNLPSDEIREYTLWGTNILGNITVTAPNGFQVSTSQT
                     AGWASTITVAADFHSPIYVRMLASAIGEYEGNIVHTSPGATPVNVLVTGEAIPAPVSW
                     NITANLTAFSSEAGTPSAIQSYTLSAPGAVEPIQITTAYPFELSSTGTGNWATSLSLS
                     YNYNGNVYVRMNASGAGTFDGVISHNTTYANEYQLPVSGTATVQPGMANDLFFSEYIE
                     GSSNNKAFEIFNGTGGPIDLSNYWVALFANGSLTPTNTLNLTGTLSHLDVYVIANDGS
                     NATILGQADITSTVTFYNGDDALLLYKKVGVDSTRIDVIGTIGTDPGAAWNVAGVTNA
                     TVDHTIIRKPTITQGTLDWAASAGTTAEDSQWVVYAIDYIANLGMHTFGNVVVMPVFN
                     PPAGAYASPINVTISTTTPGAVIHYTTDGSEPTESSAVYSAPIPISSDTTIKAKGYAT
                     GFSPSAIATAFYDFPENVATIAQLRAGTVGSAYRLTGQAVLTFQQANRNQKYVQDATA
                     AIVIDDPNGIITTTYNLYDGITGIAGTLSVYAGLLQFVPLADPGAATSHNNTIVPVTR
                     TLATITSADQAKLLKIYSVTLTPNTTGTFAATAENITATDASGTIIMRTFPGADYAGT
                     PIPTDPVDIVCLGGQYNDTMQFSPRFLADITPAAGILEAPIVNISQVGGTINLSWNAV
                     SGATNYRVESADDPYGTWTTVTTTSNLFYSGAAANKKFYRVIAIN"
     gene            complement(20648..23116)
                     /locus_tag="PLB65_03805"
     CDS             complement(20648..23116)
                     /locus_tag="PLB65_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77683.1"
                     /translation="MKKVILLATMLMSVCMFAQNWKTYTNTNHIYDLMVEGNDLYCAT
                     WGGVLKLSSITDSQDLSSYSETMVYNTGNGLVSNDIRTITYIDFSGSLWMGSSDNGIS
                     ILSQLGFQNIGSDLGLPSLKVTRIIEHESTILVATSSGLAVFYYLPGVSFPLMLHQYT
                     SANTSGGLVGDNIVDMLLTETGTLFLATTSGVSYVPLDSLDIDSAWKTIQGIENPVLS
                     GGNPILSANSESIAIGLYNKVYVHSLNMNSGSWLYYNVGNMLTGNDVSSVLLDSQSRL
                     WVAYASWKESTLSYTNTTDSLFTLIEDNTYTHILKETNGLGYSPISRILELNGKIYFC
                     SWGEGIYRQEDGYWSNYNPVCIGFPRIGQVVTDNNYKPWFASGYISDKPVRKGSMGVS
                     KFDDGAWQTLNIHNSPIHTDNILGIAVDPLNRKWFATWDNASSPEGWEKGISIYDEDN
                     NSWVHITDSGIRYYDNVSETWSDFDTSTRLTTGTIGGIYPAGDSLMMVMCYIGGVDIL
                     NMNFEKVAHFSPANTVNNRILYGFYNGQQYFIGTNDDRGLSIWNNDSIPVTGGNHWLI
                     PSPPDLNSCVVYGVVTVDTPYEGKQHWIAASTGVYMWNEIYWYRYDTMIKRYKFNPLN
                     HQWENDILYYVDEERLFGSVRTTPTCIFMDAQNRIWLGSMENGLSVYDPYTERFTNYF
                     SPNYPLMSNYITSLGFDPVAGDLIIGTPDGFNTLRIGRTIKPLTSLQNLKAFPNPFRP
                     SIHPYLQIVNMPVDNMPPGKAECRIYDTSGALVMKLEENAFSRFQWDGKNTNGKQCSS
                     GIYFFVVADEKGNVKKGKLAIVND"
     gene            complement(23113..24159)
                     /locus_tag="PLB65_03810"
     CDS             complement(23113..24159)
                     /locus_tag="PLB65_03810"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOQ77684.1"
                     /translation="MINIYLLIVLEAIVIYLATHLLFPLNLKFSNKLKLIAYPNERRI
                     NKKPIPEAGGLCFALPILIAQATFGIFSGNTEIGKMLLELAGVGFLALLFGLWDDRYE
                     SRARYKLIWQICLAVIMYLSGYKVLYLTNPFGSEFVLGWLAFPVTIFWYLLMFNAINF
                     IDGLDGLACGITIIVCAVLLTIGIKDNNQLVIAISSFLIAGNLAFLHYNFYPAKIFLG
                     ETGALFIGLNIAAISTATTAQYKGITSMTLMVPIAAMALPLIDIILAVFRRLRLGNIF
                     QADKKHIHHTMLEFGFSQRSIAIIVYFVTLMFGLIAIGFSFSSKKVLFTLLLGLFAFI
                     VIIAYLLMRQERKK"
     gene            complement(24152..25294)
                     /locus_tag="PLB65_03815"
     CDS             complement(24152..25294)
                     /locus_tag="PLB65_03815"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ77685.1"
                     /translation="MKKVLHLQMLPVMYGAQRFSLHLLESLSKDEFEIWVASKPGGEF
                     VQEVKDRGYNYIPLPTFRHQICLWDLLTFVHLLYIFKKHSFDIVHTNGSKPGFLGRLA
                     ARICKIPLIIHTSHGFSFQDNQNPIVYRFFKLAEKIGNSLGDYTVFVNNSDRFNGIGL
                     GLIKPEKALTIYNALPSKFNNELQDIAKNRKEAQQEVIIGSTLRYSTQKNVINLITAS
                     CNACLSEPKLKFIYLGDGEYYDLCQAIIHSYNLEQRILLVGWDKNVLPWLKIFNVFVL
                     YSLWEAMPFSIIEAMSSGLAIIISDIPPLSELVDKQTGYIVPLNNNAELVNTFVEIAR
                     DPKNAYKKGQLAAIKINELCNYETMVSSYRNLYRSTKPETARKNND"
     assembly_gap    25519..25618
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(25896..>27059)
                     /locus_tag="PLB65_03820"
     CDS             complement(25896..>27059)
                     /locus_tag="PLB65_03820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77686.1"
                     /translation="ETPIVDNLLWLVDSLIKEHCRTKNICIAQKMYWPMAQQAAALLS
                     HSIDDLQKWDASSLVLSLPDDLAMLFTFRWKQLAHTVKGKFQYLFTNYELNWNFDEFL
                     KLEKDSNCRSTFYLATEANEDIDYSLEDPDLQEEIQKILHNGNEIGLLATADKLTQDE
                     FLTRKQILLHLLGKEKLGIRQLGYKINNTIRDLHNKISPAFSQCTAYQSLPGYKNGMS
                     LPWQPWINGSKADFWEIPTIYRDEYLKINKHNYLQLEDAKHQLKKFFQNALRTHSIFS
                     TDFTIASFSNISYMNKLYPYLLALIKSSNTYLTTAGELVDWWEKRKRVTINENEDQIG
                     LMFPDDLESFTLCLHGNPKIKEVVEMPAKVKGNQIQFTNIKADSIAVIVLEGR"
BASE COUNT         8274 a         5390 c         4844 g         8251 t
ORIGIN      
        1 attttgaccc tcagggaaat gctaccagcg gagttggaat cgataaagat aaggtagaat
       61 tgcagatata tgatgcctta atagatagag ctccaataga aaaaacaatc ctagctactt
      121 ccacagaaaa cttatattgc attcctggaa atataaattt aacaggagcg gaaattgaac
      181 tggttcaaga atatgcgcgt gagcataaat tgaaaaatgc cttacagccg atagtaaatt
      241 cattcgaata tattataatt gattgtcctc cttcacttgg tttgttaact gttaatgcta
      301 tgacagctgc tacggaagtt ttagttccta ttcaatgcga atattatgct ttggaaggag
      361 ttagccaatt acttactact attcgtttga ttcaaaaaaa cttaaatccc ggattaaata
      421 ttctgggagt gctgttaacg atgtttgata aaagagtaaa tctatctttg caagtagcta
      481 aagaagttca tcgctatttt aaagagaaag tttttcgtac aattattccg cgcaatgtta
      541 aattaacaga ggcacctggt tttgggaaac ctatttttct ttatgatata agatctccgg
      601 gtgcgatgag ttaccttaat ctggctaatg aagttatcaa tagaagtaaa taggtagtta
      661 tttatgaatg atcgtttagg acgaggtctt tcagctctaa ttccagaaaa tgatcttgaa
      721 gaaaatccca aattgggtat tagtactctg ccgattgaca gtataaaacc caatcgttat
      781 caaccacgga aaatattcga tgcagaaaag ctggctgagc ttactgaatc catcaaagaa
      841 aatggcatta ttcaaccctt gattgttacc aagactactt cttcggaata tgaattaatc
      901 gctggagaaa gaagattgga agcagccaaa ttagctggtc tggaaaaagt gccggttgtt
      961 attcgtagtg tttcgaaaaa ggaacagctt cagtttgctt taattgaaaa tatccaacgg
     1021 gaagatttga atcctatcga agaagctctt gcctataatg ctttagttga agattttgga
     1081 ttaactcata accaaatatc tgaaattgta ggaaaagata gagctacaat aacaaattct
     1141 ctgcgtttat tgaaactacc ggaaagaatt aaacagatgc ttatttcagg agaattgaat
     1201 tcgggacagg cacgcgctgt tctttctgtg aaacctgaac tgcaaattag ttttgctgaa
     1261 tacattatta aatataattt aactgttcgt gaagctgaag ataaagcgaa aacttattct
     1321 gaagacaaaa ctgcagcacc gcaaaaagca aaaacaagtt cccgggttgt agatctggaa
     1381 aaagaattgc atcagctatt taagatgaaa gttaaggttc aagaagctaa tggtaaaggg
     1441 aaaataacct tcgtctataa atcggaggaa gaacttttga aatttaaaac tatcttagat
     1501 aaaataaggc aggaataatg aagagcgtga ttgttaccgg aggcgcaggt tttattgggg
     1561 caaactattt gcataccctt tttgccgatg agaatttttc agggaaaata ctaaatatag
     1621 ataaacttac ttatgctgga aacttggata gcttgcagga tttagagagt aaatatggaa
     1681 actctcgtta tttctttgta agggcagata tctgcgatgc tgaaaaaatg aaggaagtaa
     1741 taaatcagtt caaaccagat acaattgtcc attttgctgc cgaatcacat gtagatcgca
     1801 gtattgatgg tccgatggaa tttgttaaaa caaatctggt gggaacagct actttgctaa
     1861 atgctgcttt ggaatattat cgaactttgc aggaagccga aaagaaaaat tttcgctttc
     1921 atcatatttc tactgatgaa gtatttggct ctttaggaga atcaggtatg tttacagaag
     1981 agacagctta tgatccttct tctccctatt cagcttccaa agcaggttcc gatcatttag
     2041 tccgtgcctg gcagagaact tttggactac ctattactat ttctaattgt tccaataact
     2101 atggtcctta tcaatttcct gagaagctga tccctttaat gattctaaat tgcttggctc
     2161 ataaaccttt acctgtttac ggaaaaggac taaatgtgcg cgattggctt ttcgttacag
     2221 accactgtga agctatcaat attattatcc gtaaaggtaa atttggcgaa acctacaata
     2281 tcggaggcca caatgaaatg aaaaacatcg atattgtaac tacgatatgt aaaattttag
     2341 acgaactgga accttcaccc actctgaata gttacactga actaattacc tatgtgaaag
     2401 ataggcctgg ccatgattta cgctatgcta ttgacgccgg caaaattgaa aaggacttga
     2461 gatggaaacc tgctgaaact tttgagactg gcatcagaaa aaccatctct tggtatctgg
     2521 aaaaccgtga gtggtggcaa aatattcaaa ataataatta ccgccaagaa cgcttggggc
     2581 ttggataaaa ttaacttttc tacagaccag taagaacttc ttgacaaaat atgaatttag
     2641 catatcatat aaagaaacag caaataaatc taaaggataa aaaatgaaaa aagatgtagt
     2701 gaactacttt ctcgaactta tagctattga tagcgaatct aaaaatgagc gagcagtgat
     2761 tgataaatta aaattggatt tggaacaact gggggcaata gttgaagaag atgattgcta
     2821 taccaaaact aatggtaatg caggtaattt gtatgcctat ttccctggta caataaataa
     2881 aaaacctctt cttctttgtg cacattctga tactgtaact cccggaaaag caataaaacc
     2941 aactattgcc agtggtcgca ttttcagcga tggaactaca attttgggtg cggatgataa
     3001 atctggaatt tcagaaatta tggtagccat aaagaatatt aaagataaag gcatcgaaca
     3061 tgcacctatc gaagtgctga ttactgtttc ggaagaaata ggacttttag gggctaaatg
     3121 ctttgataaa tccaaattac aatccaaaat cggctatgca ttagacagcc atcaggttgg
     3181 tgaattaaca attggagctc cttaccaaaa tactttttgc atcacttttt atggtaaaga
     3241 agctcatgct ggagtggaac ctgaaaaagg actaaatgca attcgtttgg ccgctgagtc
     3301 cattgtagca atgcctaatg gaagaatcga ttttgaaact acctgtaatt taggagctat
     3361 ttatagtggc tcacctacca atattgtccc taaggaagtt gtggttaaag gtgaagctcg
     3421 tagccataaa aaagcgaagc tggatcaggt ttgtgacgag atcaaacaag ctgtaatttc
     3481 tactgttaaa cgctataatg accaaaatgg taatgcctct tacaaatttg aattggaaca
     3541 agaatatgag gcatttttta tcccaaataa tgctccagtt gtttctcttg ctgaaaatgc
     3601 tctacgcgct ttagatattc cttcgaatat aaaaattgga gggggtggta gtgatgctaa
     3661 catctttaat gccggaggca tacaaatgat catagttggc acagggatga acaatgttca
     3721 tactgtaaat gaagatatta ctgtagatca actttataaa ggttctgcat ttgtagaaca
     3781 gttaatcatt caatatgcgg agaattaaat gggactaaaa ctgtgtttaa ttggttatgg
     3841 gaaaatggga aaaatgctgg caaatttagc cactaactat gattgcacag ttgtttctat
     3901 tatagatcct caacaaaata actaccacag tgaaatttcg ttgcaatcaa ttggcgaggc
     3961 agaagtttgt atcgatttca gccacccttc agtagtgctg gataatattc aaaaagtgct
     4021 ttcctataag aaaaatatgg ttgtgggaac taccggctgg tttgatcatt tagaggaagt
     4081 acaaaaaatg gtggaaaatt cggcttcagg tctgatttat ggagctaatt tctccattgg
     4141 tatgaatatc tttaatcgct tagtagcaga agcaacgaaa gcctttgatc gttttcccga
     4201 ttatgatgtc tacggtttgg aaatgcatca taatcaaaaa gcagatagtc cttccggaac
     4261 agctattgag ctttccaaaa ctatattagc caacagcagc cataaaaaaa ctactctatt
     4321 tgatcctgca tatagaaaaa ttgaaccgag tgaattacat tttgtttcat tacgagccgg
     4381 ttcaattcca ggaacacata aagtcggctt tgatagtgaa gccgacacaa ttgagcttag
     4441 tcattgtgtg cgttctcgta gtgcattagc ttctggtgct ttacaggcag caaaatggat
     4501 taaagaccgc aagggctttt atagctttag cgatatggtt tcttccgttt tatgctgata
     4561 ccttttatta aaatgaatgc acagggtaac gattttgtta ttctggatta ttttgcgcct
     4621 gaacttgtta ctttaaagga acttaattat tcctccctgg ctttagaagt atgtagacct
     4681 cattttggca ttggggctga tggcttagtt ttattaaagc cctccacaga atttgatgct
     4741 caaatgataa ttttcaatgc cgatggttca caagctgaaa tgtgtggctc tgctttacga
     4801 tgtgtaacct ggcttctata caagaaaaca gaaaaaaggg aatttgagat tcttactgat
     4861 tctggagtta aaaaatgcca ggtaaacaac gataaaacaa taactgctaa tttgggttta
     4921 cccaaaatgc taaaaagaaa gtacaatgct gagggcttca caggagatct gattgaaatt
     4981 ggcaatcccc attttattgt ttggcagaat aattctgatg atgacccaca tataaaatat
     5041 ggtccacttt tggaacatca ccagggtttt cccgatactg tgaatgttca ttttgcttac
     5101 ctgataaata gaaatgagat agctatcaaa atttgggaaa aaggttgtgg agcaactttt
     5161 gcctgtggaa caggagcttc agcaactgtc tatagtggta tcattaatgg ctttttggat
     5221 aacgaagtta aggttaatat gccaggagga actgtaaaaa tttcagccac cgcacagggg
     5281 tatttactaa gtggggaagt ttatgaaacc ttccaaggag tattcaaatg gagaatttag
     5341 gtaagtacct tctggaattg cgtttacagc gtggttacag ctatcaggat atctggaatg
     5401 aacttcgttt caaagaagat cagataaaag caattgaaga aaatcgctta tctgcattag
     5461 gaccctatgg cattgcaaaa gctgttgttt ttaattatgc tcgttacctt gaagccgatt
     5521 tgaattctgt gatgaatgcc ttttccatta tgatgccgga aagtaataaa cctgaattta
     5581 aacccgctac cacagttaaa gaaaaaaaga ttatgctctc caccaatttt atgtggaccc
     5641 tggggatttt agttattatt attatcctgg gttcaattct ctggtatgcc tataatcaaa
     5701 attggcttag acccccgaat ttcttcaccg ccaaaaaatc cgattctaca gctgttagca
     5761 tcaaagagca aaaagaagaa acaaaacctg atactttgcg tcaacatcta caggcaatta
     5821 gtgaagccat acctaaaact aataatataa aaattgataa tttagatagc aaaactgtcc
     5881 ctccagatac aactgattat cttggcaata ttttgggtga tagccctatt aatgtcccta
     5941 tccattagcc agattattat cttacagcta ccctctaaga actttttaaa aaatccaaat
     6001 ctgagatagt gaaaaattat aatttttttg ctattgtaac atctttatat tcagttagtt
     6061 agctaattcg gcaaaaagaa tttagcaatc taatggtcag attgctaata ttttccttgc
     6121 tttttttgtg ccttcgatta tattaattag tgaacaataa aaattcaagg aggttcaaaa
     6181 tgaaactcgt accttatcgt agaactaatg aactaagacc aatgagcagt atgctcaatt
     6241 tattcgacga ttttttcaat cgcttttacg aagaggaagt tggagatgat aatttccaac
     6301 cgatggcaat ggatataatt gaacaggata aggaattcca ggttttggct aatctgcctg
     6361 gatttaagaa agatgatgta aaaataagta ttcatgataa tcaattaata attgaggcca
     6421 attgcaatac taccaaagaa gagacaaaag gaactgtata tcgctgcgaa agatacagtg
     6481 gcagctatcg cagaaatctt gtccttcctg aaaatgccga gatttctaaa atctctgcta
     6541 agatggaaga tggagtgttg aaactaacta ttccgaagaa agaaccctct cccaagaaag
     6601 agattagcat tcagtaatct ctggaaacaa acaaactatg aggattgcgc ctagctggcg
     6661 cagtcctttt tttgtagtta aagctaattt atgaaacaag aaaagtgttg caaaaatgta
     6721 aaagtataaa gtgcacgcta agcaattaag cgtgagaagt ggttgatgaa gacatcaatt
     6781 ttcttgtttt tttccagaga ttacgccacc atcgctatga ttgtgttggt ctttgagtta
     6841 aaatcggttt tgcttgtgat ctgccataca atttcgttct ttttgctttc tttgaataag
     6901 gtcattaatt atctcataga aaatgccgat tttgacttaa agccgacagg atnnnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaagactt ctcgactcca agacccctcg
     7081 acttctcgtc ccttcagtta cattttcaga agcttgtgga tgatcgttct ttgctcactt
     7141 ttaactatta taaggtaaat accggaagga acagccctat ttttattatc caagccatcc
     7201 caggcaaaat gttgtaaacc tgtatccaaa ggaatagttt ccattttcct aattagctgt
     7261 cctttaatgt tatagatgta tattttactt tccgttgcag aactaagttt tagttccaat
     7321 accacagacg aactgaaggg atttggatag ctatgtaaat attcaatttt atcaggtggc
     7381 agaggaggat tttccggttc aggattaggt aaaatcagaa ctatcggacc caaaacattg
     7441 ttaattccat tataggcaat tgtttccagc caatagtaaa gtgtatcggc aaggggcaat
     7501 tcagaatcat aaaaactata atgatagccg cttgcaccgc ctattgcaga aattatggaa
     7561 gcattcaatt ttagggcaga agaaaaatca ggcctatcat ttctgtagag attatagcca
     7621 gcgagattag tttcagaagc tgtattccaa aaaatattgg caccattagc atagacagca
     7681 tagaaagcag taagttctac aggcagtgta acttcttctg ccgtaaagaa atccataatg
     7741 catttcagga ggtttgctct cgtattagga aaagttccat ccgttaattc acttaaagca
     7801 tagctaaaca caaagctgcg ttcattatag ccacctgcac tggcaactgc cacacatcca
     7861 taatcacttt cctcaaaagc acttctggca taaggataaa gagccgtaaa acgatctata
     7921 taatccactt tagtttgcgt ggaaccagta aaattcattc cggaagttgg agtattagaa
     7981 acgccgctaa gattattgat agcattggtt tgtccatcct cagcattcat tattcccaag
     8041 agaggcaaaa gaacttcatc cgcatcttgc tctccttgaa catccggaag atagtatcca
     8101 aaatcgaatc cgaccacatc cacgccttca atatatactt ttccgccaga aattaaataa
     8161 ctgtataacg cattatacat aagtaggtta tcaaaacgaa caatgttact gcccactgct
     8221 ccaaaactta aaaagaccgc ttcaaaactg taaaacgaac tggggaaagt agttccgtaa
     8281 acacagtcat aacccaaatc ctgcaaagtg ttattaataa aggttccgct catatctctt
     8341 cccccggcaa ctccttccca cacaaatatt cctcccgcat taattaatat actaaaagac
     8401 agattagctc cagcaactac aggataatcg gcaactgtat ttaaagtaaa agtaacatat
     8461 tgagaagttg cacttggcaa aacataaatg ctaaatacat tttccagaag aagataacca
     8521 tcggcaggaa tagtttgact atggcttccg gataggatat ttacatatga attgcctgtg
     8581 gaaagcgtaa aattaccatt ggaagcatat tctccgtagc ttcttaacag caaattgacc
     8641 gagaatgtct catcaggttc aaccgccaaa tttccatttg catcggttgc tcctctattt
     8701 tctattaaag caagccgaat ttcaacatcg ggaaggggat tgctttcttg caacgcacgg
     8761 cgggcattaa gttttccttc acccaaaaga ttttccctgc caagatttac ggcatctaca
     8821 tcatcgcagc tacctttgat tctatctctc agttctgttt ggcttatttc cggaagatag
     8881 gattggatta atcctgccaa agaacttgcc accggactgg cataagatgt tgcattactt
     8941 acagttgtat aaccactggc agttactgtt ccaattgatt ctgtaggagc gcctacatca
     9001 acaaaggaac cataactgga aacagaagat tttatgccat tattttgcaa agcggcaaca
     9061 gctataacat tttgataggc agcaggatag acagggatag cgttattatt atttccagca
     9121 gaggcaacaa ttgttgagcc caaagaatag gcataattga tagcttcttg atttgcccaa
     9181 gaaaaagaag agccacccca actgcaattt attatatcag ctcccatttc tgcagcataa
     9241 ataataccat cgtaacctct atatatagaa ctggaaccag ggaaactaca ggatatggga
     9301 agcattttta aattccaggc aagggaacta accccaatta tgttatttgt tctggcagaa
     9361 gcaattccac aaaccgtagt cccatgcccg ctgctttcaa aaggattatt tgcctcttcg
     9421 ccagaactgg taagcataaa atcccaacca atcaggtcat cgatttttcc atttccgtca
     9481 tcatcaattc cattcaggtc tcccgcatcc ataacccaag tgcttccgtt ataataaatt
     9541 gtatagccat tgtcgtttac atcttcgcct aaattttgcc aaatattatc aacaaggtct
     9601 ggatgagtcc accggcaacc ggtatctaca atggcaagca gcacagaatt tgagccattt
     9661 tcgcctttat gaatatccca cgcggattct gcttccagag aagaaaaata gagtgactct
     9721 gcataatttt catcattggg aatggcaaaa atgctatcgg gataaacaat ttccgcatat
     9781 tgcacataat tatctgtggc aagcaagttt acaattgtct gaggagggta gggactttgc
     9841 actgtaaaaa ttagttccag ctcacaatta tttctctttt gaggagatag attaaatttg
     9901 gggtggatgg atgagacctg taatttctgc agtttctcat caattgtggc aatgccagtg
     9961 cgattattgt ttttttgcag agcggaacgc atttctggct taattttaaa aaccgcaatt
    10021 ccttctctgt cattaaaatc gtaggcaaaa agagaaactg tcattagcag acataaaagc
    10081 agagcaaagt gcttcattct aacctcctaa aaaaccgatt cgcggctcct tttaaagatg
    10141 tttttgccat ctttataaaa acggggaatg atatgtaaat gaaaatgggg gatactttgt
    10201 ccggcagcag aaccacaatt catcgccaaa ttgtagccct gcggattata cttttccgcc
    10261 aacaactttt gcacagttat tgtaatagac tgcaatgata caacttccgc ttcggttaag
    10321 gcaaaaaaat cgggggtatg ccgtttagca attattaaag tatgcccctt actgaccgga
    10381 tatttatccc aaattgcaaa aaaatgttcg ttttcagcaa ggtatataga gcaatcaagg
    10441 ttacaaaatt cacacgccat cttttattac ctctttgtta tataataatc cagttcttac
    10501 agatctccaa atgatataat tacttattaa atatcaagtt ccttacaagt atttcctcat
    10561 cctcccgtta tcctcccgtc ctcaactcgt tctcaacgat gagcaaaaga gaggtgaatg
    10621 atgagaggag gataggattt ttgcaaggat gctttgcccg cttctaaaca agttttcata
    10681 atgtaaaggt tatattggta tagattatcc ttacttagag cttttagatg gggcagacat
    10741 accattttgg cattgtcaac attccgtgcc taagtcctct agtggaaacg cgaaatctat
    10801 cttttccgct tttttttaat agagcggaaa aaagcaagac agcggctaac attatatccg
    10861 ctctttcttt ttccattccg gcaatatttt cgcgttgctt taggtctgtc tgtttgtata
    10921 attcaatttg gcgttgaagt tccgctaagg taatttcata gccctcagct tgttgagggg
    10981 ttttaatttt gccttttgag gcaacgcagg cacaaactaa agcccctcct ccggttccga
    11041 ttaaagtatc ttttgtaact atggaaacag gtagaacacc attaatatag aataataaat
    11101 tattatagtc cttggtggaa atcggatcgc ttttcacaaa ctgtttggtc agctgaagtg
    11161 cgcctaaagg aaaactttgg ctgctttgaa tatgtccccc acggccagaa atgatttccg
    11221 tgctaaaacc cccgctgtca aaaacggtta ttggttgcaa aggattgggc aaaccgctca
    11281 gcacgccttt ccattctaat tttgcctctt gcttaggagt aaggattttc acttccaggc
    11341 agtcctgtat atatattctt cttacaaact ctttcctatt ggatgctcga cgaaaggttt
    11401 cggtgcctgc ggcaattatt tcttccacct tgtaagctct ttctatgtct aattgcaaac
    11461 aatttagaac ataaattgcc ttattttgtc ccaattcaga tatatatccc tttttggtaa
    11521 gataggaacc cagacgaaaa ggaattctaa tatccgttaa acaattaaaa ctcttctccc
    11581 ttttgtctgc aatgagacat tttatggaat tggaaccaat atccaatgct tctacaatca
    11641 ttgtagttgt tggctctgat tttaatttat gctgaagact ggcatccttg ggctacttta
    11701 tttatccaac cgctataatt ctggatacag tggaaaccga tcagttaaag aatgcacttc
    11761 ttttttcagt gcttgcaggt attcttcatt ttcaaagtta tcacgcactt ttttaataag
    11821 ctcagctatc agctccattt ctgcttttcc cattccgcga gttgtaacag ctggtgttcc
    11881 taaacgaatt ccggaagcta caaatggact tctggtatca aaaggaaccg tatttttatt
    11941 tgctgtaatt cctgccaagt caagagcttc ttccattttt ttgccgctgg ggcttccctc
    12001 aatttcgggt cctaaatcaa tcagcataag atgtgtatct gttcctcctg aaaccagttt
    12061 gaaaccttga tcaattaatg ccttagctaa agcattagca ttttccacta ctttttgctg
    12121 atagagttta aattctggct gaagtgcttc aagaaaagca actgcttttg cggcaataat
    12181 atgcattaag ggtccacctt gcacacctgg aaagaccttg ctatcaattt ctttggcaaa
    12241 ttcttcttta cataaaataa gtccagcacg aggtccgcgc aatgtcttat gtgttgtggt
    12301 agtaactaca tctgcatagg ggacaggact ttgatgtaaa ccggcagcta caagtcctgc
    12361 aatatgagcc atatcaacca ttaatttggc tcctacttta tcggcaattt cacgaaatct
    12421 ggcaaaatcc agtttacgcg gataagcaga agcaccagct aaaatcattt gtggcttatg
    12481 ttcaattgcc aaagcttcca gttcatcata atttagctgt tcagtttctt tatttacagt
    12541 gtaggggaca atattgtaaa gctgtccact aaaagaaaga ggatggccat gggttaaatg
    12601 tcctccatgg gcaagttcca aagccataac agtgtcccct ggtttaacaa gagtaaaata
    12661 ggcagccata tttgcctgag aaccgctatg aggttgaaca ttagcatgct cagctccaaa
    12721 gatttgtttg gcgcgttcaa tagctaaacg ttcagcatcg ttaataaatt cgcaaccacc
    12781 ataataacga ccgtagagtt tataatttat tgcaccagtt tttttactcc agcggtaggg
    12841 ataaccctcg gcatacttat tggtcataat acaaccttga gcttctaaaa cagccaggga
    12901 agtgaaattt tcactggcga tcagttccaa attttcacgt tctctattaa gttcattcat
    12961 aatagcagcg taaatttcgg gatcggtttt ttgaatgtga atcattactt ttctccttga
    13021 tgtattttat taattctctt taagtgtctt ccaccttcga aagatgtgtt caaccaaacc
    13081 ttggtaattt ctatggcata aggtatggcg gtaaatcttc ctgccaaaac caaaatatta
    13141 gaatcgttat gttttcggga aagacgagct atatcagtat ttacacatag agcagctcta
    13201 attcctgaaa ccttattggc agtaatactc attccaattc cgctgccaca aataaggata
    13261 cctttttcac aatgttttgt ggcaactgct ctggcagcgg gataacctgt gtctggataa
    13321 tccatactct gtgtactatg tgtgccaaaa tcctcaaatt caatttcagg aaaggcattt
    13381 atgattgctt ctttcatttc ataaccagca tgatctgaag caatggctat cttcattttc
    13441 cctccgccat taaacaagat agagctatac agtaatattt ggaattttcg ctttcgctac
    13501 ggctcataac tatcaccggt tttgtagttc cgcagatcaa accggccaat tctccttccc
    13561 caaaaagcat tagcccctta taaaaactat tggcgctttc caaattcggg aaaatgagaa
    13621 tatcggcatc accctctaaa gaactttgaa ttcctttgat ggaaagtgat gtcggatcac
    13681 aagctaaaaa tatatctaag ggcccatcca caaggcaatt ttttatctgt ccccgttcgg
    13741 ccattttaca taaaagcgaa tattccaagg tctccggcat tgaagagcta accttttctg
    13801 tggcagaaat taaagcaact ttgggttttt ctatgccaaa cttccttgcc atagcaacgc
    13861 tgtaattgat catagctatt ttttgattca gatcgggatt tattagcaca gcagtatcgc
    13921 taacaaaaag caatttatga cacttaggga tttctaaggc gcaagtatag ctcattaccg
    13981 ctttgggagg tagaagccct ttttctttat ccaaaaccgc tttcaggaaa gtttcagttc
    14041 ccactaaacc tttcatcaaa acatcagctt cattattgcg aaccattcta acagcttctt
    14101 taacagccat agcaggatca gttatattca ttatttgcaa tttgttggaa tctatgtcgg
    14161 caggtaagag agctgtgatt ttatctttat ttcctattaa gataggggaa acaaatccat
    14221 tgtttgtagc ttttaaaatg gcattgatag tattattatc ttctgcaacg gcaactgcga
    14281 tgcgggtttt tttgccgctg tttatagctt tttctgcaag ttcagtaagt ttcgtaatca
    14341 tttttcttac gccaacatcg ctgctaaagc gatagaatat aatttactgc attcgctatc
    14401 accccgggag gttaaaacac agggaacccg cgcccccata actatcgctc ccagttcact
    14461 tttcgccagt tttatgatag ttttgtaaaa agtgttgcca gattcaatat tggggaaaag
    14521 gatacaatct gcatctccac agacaggact atccacacct ttaattaggg cactttctct
    14581 atccaaaatc atatctaaac ccaaaggacc ttctactatg gcacctttaa tttgaccgcg
    14641 ttgagccatt ttggcaataa ttgcggcatc cgcagaagaa gtaattttgg gaagggtttg
    14701 ttcactggca gaaagaagac ctactttggg attttcaata cccaataaat tggcagtcct
    14761 aatcagataa ttagttatgg caattttctg atttagatcg ggaagaggaa taatagctac
    14821 atcaccaact atcagtaatt tatgataatg ctttgattcg gtaactgtta catgggaaag
    14881 aatcgcacca ggatccataa gcccgcattc tttatttaaa attgctttca tataacgatc
    14941 ggtggaaata agccccttca ttaaaaaatc gcccttgcct ttattaatca tttctacagc
    15001 taggctagca gatttaatat ctacagggca atgaacaatg ttaaatttgg aacaatcaag
    15061 attatgttcc ttacacactg ataaaatctt tttttcatca ccaactaaag ttgcttcaat
    15121 cagttccttt tccacagcca tatatgctgc taaaatagta tgtgaatcaa ctccccaggc
    15181 cgctatcata cgtttttttg gcctggtagc caaaacatcg aacatttggt caagtttgct
    15241 aatctgcatt ttttttgtcc tttagatctt tatttttatt aaactttttg tttcatcata
    15301 aacacaagcc atttcctgca aattatatag cttcagctct aaaaacggaa ttccattctc
    15361 tattaaatat cggctctgta aaagaaaatc tgccccacta tgctctctaa catataataa
    15421 ggaatcaggg tgtgaagata atataagcga agtatcacat aaaaccaaat tgactgcata
    15481 acttcttaat agttcgtcct ttattaattc taaaagagaa gattcctgct ctgcatattc
    15541 tgattctatg ttagaagaaa ttagcagaat aaaaggtctg cgctcctgct ggaatttact
    15601 aagccaatca ccttccagaa gagcttgcac attttcgata acagtataaa catacctctg
    15661 ctcttcaatt atctctgtag aagatacgga ttctgaaaca taaattttat cctctgtttc
    15721 tatctctgga ataatatatt catcaatgct atccttactg gctttaattt tatacgcttc
    15781 ttcttcactc ttgtttttct gaaaagaagt acaggcaaaa cataacaagg atactgcaca
    15841 aataattaac ctctggataa aatgcatctt catcctttta ttacaactgt atgagccatt
    15901 gcaagttaac ttacttccaa ataaagttac catatttgaa cattatgtta ttaattcaca
    15961 tttctgtcaa gagttagttt cgcaaagctt ggcactcctt ccacaatttc aaggcaataa
    16021 gctaatttta catagaaatt ttctcaggac tacattgata taactttgca atctgtgaaa
    16081 gacagataat ctatcttggt ttagataatt ttacttgacg gcatagctat atttaattag
    16141 attatcattt acgagtaaat ttaggtatca gataaaaata gcttaattct acagggagga
    16201 attatgaaaa agacattatt tgtaatgctt ttagcccttg cctttttagg aagtttgtct
    16261 gctcagataa atattcaatc tgggcaaacg cttactcagg attttgatgt tttaggaact
    16321 tccgccactg caacaatgcc tgccagttgg aaagtagata aaaataccac agtccgattg
    16381 gttggaaatt attctgcagc tgttactgcc acagaaagaa ctggtggtaa taatatgtct
    16441 actaccgccg gaaatggcat ctataattat ggagcaggac cagcagatac tgctaccgac
    16501 cgcgcagttg gttttttatc ttccggctct gccacaaaaa gtggtaatgt atatgttcag
    16561 cttagtaata acggttccac gacaatcaac tcttttgcta tcagttataa tgtggaaaaa
    16621 taccggatgg gaactaatgc agccggtttt tccatccaaa tgtattactc cactgatgga
    16681 attacttgga caagtgcagg aagttcattt ttaacttctt tcccagcgga tgctacaact
    16741 aatggttatg cttctgctcc gggagcaaca attcctgtaa atgccacttt atcttactct
    16801 ctttctgcag gaagttcttt atatcttgct tggaattatt ccgtaacttc tggaacttat
    16861 acttcctatg cacaggcatt gggtattgat gatgtaagca taactgccgg aggaacagct
    16921 tcacctacca ttgtagttag tggcacttta actccttttt ccacttatac cggcactcct
    16981 tcaacagctc agagctacac tttgagcggg caaaatttaa ccggaagcat cactgttgcc
    17041 gcccccacag gttttgctct atctactgat aacactactt tttcaagcac tcttacctta
    17101 gccagtaatt ttaatgggct tgtttatgtg cgtttaactg gaatcacggc aggaactttt
    17161 tccggcaata ttgtccattc cagttccgga gcaactacta taaaccttgc cgtggaagga
    17221 acagttacca atccttcccc attgatagat attactgcca atttaagtga tttcagtaca
    17281 attttgggaa ctccttctgc cgcacaaagt tatacggtaa ccggacactt cttaacttct
    17341 gctattagta taactgctcc cacaggtttt aatctttcta cggataacac aacttttact
    17401 tccactcttt ctttggcaag tgatttcagc ggaactattt atgtccgttt aacaggtgtt
    17461 tcagcaggaa cttttactgg caacattgtc catagtagca caggagcccc aattcaaaac
    17521 ttagccgtaa acggaacagt aacagaccca agtgttcata atactttttt ggacgaggaa
    17581 tttatttatc ccgcaggaga tctattaact gcccatggtt ggaatgcaca tagtnnnnnn
    17641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaaaacc tttgctaatc agacctcagg
    17761 cactttttac gctgcttttc tgttcaatgc ttccgaagct aaaactaccg ctgattattt
    17821 cttccatttt ggacctaatc ctattggttc tgatttaaaa ggcagagtat ttgcccaaaa
    17881 ggatgcttcc gataacctaa gattcggtat aaccaaagca gcaggtgcta tttcagaaac
    17941 tgctgctact ccttacagct atgctctaaa tactacttat ctgattgtta ttaaatatgt
    18001 tattgtttcg ggagcagcta acgatgaagt ctatatgtgg gtgaatcctg ttatcggagc
    18061 tacagaacca gctgcgcaac ttacagcagc tgatttatca ggaaccgaca ttgcaggaat
    18121 tggagcaatt gctattcgcc aaggagctaa cactcccatc gccagaattg acggcatcag
    18181 agtaactaat gattgggcaa ctctttggga aggaactcct atggaaactc ctgttattca
    18241 taccagcaca cccgaacttg atcctttagc aagcatagtt aatttacctt ccgacgaaat
    18301 cagagagtac actttatggg gaaccaacat cttaggcaac ataacagtta cagctcccaa
    18361 tggttttcag gtttctactt cgcaaacagc aggatgggct tccacgataa ctgttgccgc
    18421 tgattttcac tctccaattt atgtgcggat gttggcttct gccatcggtg aatacgaagg
    18481 caatattgtt cataccagtc caggagctac accggttaat gttttggtta ccggcgaagc
    18541 aattcctgca ccggtttctt ggaatattac agccaattta actgccttta gttccgaagc
    18601 aggaactcct tctgcaattc aatcatatac tctaagtgca ccaggagctg ttgaacccat
    18661 tcaaataacc actgcttatc catttgaatt aagttctact ggcacaggca actgggctac
    18721 atcattgtcc ttaagctaca attataacgg caatgtctat gtgcggatga atgcatcagg
    18781 agcaggaact tttgacggtg taatttccca caatactact tatgctaatg aatatcaact
    18841 gcctgttagt ggaaccgcta cagttcaacc cggaatggca aatgacttat tcttctcgga
    18901 atatattgaa ggcagcagta acaataaagc atttgaaatc ttcaatggaa ccggcggacc
    18961 gattgacttg agtaattatt gggtagccct ttttgccaac ggctctttaa ctcctacaaa
    19021 tactttaaat ctaactggaa ctctttctca tttggatgtt tatgttattg ccaatgatgg
    19081 ttccaatgca accatactgg ggcaagcgga tataacttcc accgttactt tttataatgg
    19141 agatgatgct cttttgcttt acaaaaaagt tggggttgat tccaccagga tagatgttat
    19201 cggcacgatt ggaacagacc caggagcagc atggaatgtg gcaggagtta caaatgctac
    19261 agtagatcat actatcattc gtaaaccgac tattacacaa ggcactttgg attgggcagc
    19321 cagtgccgga actactgcag aagatagtca gtgggttgtt tatgctatag attatattgc
    19381 taatttggga atgcatacct ttggcaatgt ggtagttatg cctgttttca atcctcctgc
    19441 tggtgcttat gccagtccta tcaatgtaac gatcagcaca actactcctg gagctgttat
    19501 tcactatact acagatggtt ccgagcccac agagtcctct gctgtttaca gcgctccaat
    19561 tcctatcagt tcagatacaa ccataaaagc taagggttat gccaccggtt tttcaccttc
    19621 ggcaattgcc actgcttttt atgattttcc agaaaatgta gctaccatag cccaactccg
    19681 agctggaact gtaggtagtg cttatcgtct tactggtcaa gcagttctta ctttccagca
    19741 agcaaatcgt aaccaaaaat atgttcagga tgctactgca gctatcgtta ttgacgaccc
    19801 gaacgggata ataactacaa cctacaatct ctatgacggc attaccggta tagcaggaac
    19861 tttatctgta tatgcaggtc ttttacaatt cgttcctctt gccgatccgg gtgctgccac
    19921 ttctcataat aataccattg ttccagtaac aagaactttg gctactatca cttcagccga
    19981 tcaggcaaag ctgctaaaga tttacagtgt aactttaacc cccaatacta caggtacttt
    20041 tgctgccaca gcagaaaaca tcactgccac ggatgcctcc ggaacaataa taatgagaac
    20101 tttccccggt gccgattatg ccggaacccc aattccaaca gaccctgtgg atattgtctg
    20161 tttgggaggc caatataatg atacaatgca atttagcccc cgttttctgg cagatataac
    20221 ccccgctgca ggaattttgg aagcgccaat tgttaatatc agtcaggttg gaggaacgat
    20281 aaatttaagc tggaacgctg tttccggtgc cacaaattat cgagtagaat ccgcagatga
    20341 tccttatgga acctggacta cagtaacaac tacttccaat ctattctata gtggagcagc
    20401 tgctaataaa aagttctacc gggttattgc tattaattaa ataagcaata tctgtttaaa
    20461 tgaaagggtg gagtatatgg ctccaccctt ttttatcggt ttacgattat aagatattta
    20521 caccttggaa gttatcaaca gattcaaaag ctatttaggc acaaatgatc cctttgatca
    20581 agcttctcaa tcctgaaatg ccaatatcct cgtttctaca atcaaatctg ctttagcact
    20641 tccccaatca atcattcaca attgccagtt tacctttttt cacattacct ttttcgtctg
    20701 ccactacaaa gaagtatatt ccgctgctgc actgttttcc gtttgtgttt tttccatccc
    20761 actgaaaacg actgaaggca ttttcttcca atttcattac taacgctccg gatgtatcat
    20821 aaattctaca ttctgctttt ccaggaggca tattatccac gggcatattc acaatttgca
    20881 aatagggatg tatagatggt ctgaacggat tgggaaatgc cttcaaattt tgtaaagaag
    20941 tgaggggttt aatcgttctg ccaattctaa gagtgttaaa tccatctggg gttccgataa
    21001 ttaggtctcc agcaactgga tcaaaaccaa gcgaagttat ataattggac attagaggat
    21061 aatttggaga aaaataatta gtaaaccttt ctgtatacgg atcgtaaact gagagtccat
    21121 tttccatact ccccagccaa attctatttt gcgcatccat gaaaatgcag gtaggggtag
    21181 ttcgcacaga tccaaacaag cgttcttcat ccacataata cagaatatcg ttctcccact
    21241 gatggtttag cggattaaat ttatagcgtt tgatcattgt atcatagcga taccaataaa
    21301 tttcattcca catataaaca cccgtgctgg cagcaatcca atgttgtttt ccttcataag
    21361 gagtatctac agtaaccaca ccatacacta cacaactatt caaatcaggc ggagaaggaa
    21421 tcaaccaatg atttcctcca gttacaggaa tagaatcatt attccagata gaaagcccac
    21481 gatcatcatt tgttccaata aaatattgct gtccattata aaatccatag agaatacgat
    21541 tatttacagt attggcaggt gaaaaatggg caactttctc aaaattcata ttcagaatat
    21601 ccactcctcc aatataacac atcaccatca ttaaagaatc tcctgctgga tagattcctc
    21661 caattgtgcc agttgttaaa cgcgtagaag tatcaaaatc actccaggtt tcggaaacat
    21721 tatcataata acggatcccg ctatctgtaa tatgcaccca ggaattatta tcttcatcat
    21781 agatacttat tcctttttcc caaccttcag ggctggaagc attatcccaa gtagcaaacc
    21841 attttctgtt taagggatca acagcaattc ctaaaatatt atccgtatga attgggctgt
    21901 tatgaatatt caaggtttgc caagcaccat catcaaattt actaactccc atagaacctt
    21961 ttcgtactgg tttatctgag atataaccac tggcaaacca gggcttataa ttattgtcag
    22021 ttaccacttg accaattctg ggaaaaccga tacagacagg attatagtta ctccaatagc
    22081 catcttcttg tctataaatt ccctcccccc agctacaaaa atatatctta ccgtttaatt
    22141 ccaaaattct actgatgggg ctatagccca ggccgtttgt ttctttcaga atatgggtat
    22201 aggtattatc ttctatcaaa gtgaaaagag aatcggttgt attcgtgtaa cttaaggttg
    22261 attctttcca actggcatac gcaacccata aacgggattg ggaatccaac aaaacagaag
    22321 aaacatcatt accagttaac atattgccta cattataata tagccaggaa ccgctgttca
    22381 tatttagaga atgcacatag accttattat aaagaccgat ggcaatactt tcactatttg
    22441 ccgataaaat tggatttcct ccacttaaaa cagggttctc gattccctgt atcgttttcc
    22501 aagcagaatc tatatctaag gaatccaagg ggacataact tacgccgctg gttgtggcta
    22561 aaaaaagtgt tcccgtttct gtaagtaaca tatctacaat attatctcca accagtccac
    22621 cggaagtatt ggcagaagta tattgatgca acatcaaagg gaaagaaaca cccggtagat
    22681 aataaaaaac tgcaagccca ctggaggtgg caactaaaat tgtagattcg tgttcaatta
    22741 ttctggtaac ttttaatgat ggcaaaccca aatcgctacc aatattctga aaccctaatt
    22801 gacttaagat agagattcca ttatccgacg agcccatcca caaacttccc gaaaaatcaa
    22861 tataagtgat agttcttatg tcattggaaa ctaatccgtt gcctgtatta tataccattg
    22921 tttccgaata agaactgagg tcttgggaat cagttatcga actcaatttt agcactccac
    22981 cccaagtggc acagtataga tcatttcctt ctaccattaa gtcataaata tgattggtat
    23041 tagtgtaggt cttccagttt tgagcaaaca tacatacgct catcagcatt gtagctaata
    23101 atattacctt tttcattttt ttcgctcctg tcgcataagt aaataggcaa taattactat
    23161 aaaggcaaaa agccctaata gcaaagtaaa aagcactttt ttggatgaaa aggaaaagcc
    23221 gatagcaatc aatccaaaca tcaaggttac aaaatataca ataattgcaa ttgatctttg
    23281 ggaaaagcca aattccaaca ttgtatgatg aatgtgcttt ttatctgcct gaaatatatt
    23341 tcccaagcgt aaacgccgaa aaacagctaa aataatatct attaaaggta aagccattgc
    23401 agctatgggt accattaaag tcattgaagt tataccttta tattgagcgg tagttgcagt
    23461 tgaaattgct gctatattaa gcccaataaa taaagctccc gtctctccca aaaagatctt
    23521 tgcaggatag aagttataat gcaaaaaagc taaattgcct gcaatcaaaa aactggaaat
    23581 agcaatcacc agctgattat tatcttttat tccaatagta agcaacaccg cacacacaat
    23641 aatcgttatc ccacaagcca aaccatccag tccatcaata aaattaatcg cattgaacat
    23701 tagcagatac cagaaaatag ttacgggaaa agctaaccag cccaaaacaa attcactgcc
    23761 aaagggatta gttaaatata aaaccttata gccacttaga tacattatta ctgctaagca
    23821 tatctgccat ataagtttat agcgagcacg gctttcatat cgatcatccc aaagtccaaa
    23881 aagaagagcc agaaaaccca ctcccgcaag ctccaaaagc atttttccga tttccgtatt
    23941 accactgaaa atgccaaaag ttgcttgcgc aatcaagatt ggcaatgcaa aacaaagtcc
    24001 acctgcttcc ggaatcggtt ttttgtttat tctgcgttca ttgggatagg caatcagctt
    24061 cagcttgttg gaaaatttca agtttaaagg gaaaagcaga tgtgtagcaa gataaattac
    24121 aatcgcttcc agcacaatta gaaggtagat attaatcatt atttttcctt gcagtttcgg
    24181 gtttggtaga tcgatacagg tttctataag aactgaccat tgtttcataa ttacatagct
    24241 catttatctt tatagctgcc agctgacctt ttttgtatgc gttttttggg tctctggcta
    24301 tttctacaaa ggtattcacc aattcggcat tgttatttaa aggaactatg tatcctgttt
    24361 gtttatcaac aagttcagaa aggggaggaa tatcagaaat aattatagct agcccagaac
    24421 tcattgcctc gattatactg aagggcattg cttcccaaag cgaatacaga acaaagacat
    24481 tgaatatttt cagccagggc agcacatttt tatcccaccc tacaagcaaa atacgctgtt
    24541 ccaaattgta actatgaata atcgcttggc acaaatcata atattcgcca tccccaagat
    24601 agatgaattt gagctttggt tcgcttaaac acgcattaca agaggctgta attaaattta
    24661 taacattttt ttgagtggaa tagcgtaaag tggaaccgat aataacttct tgctgggctt
    24721 cctttctatt tttggcaata tcttgcagct cattattaaa tttgctgggc aaggcattat
    24781 agattgttaa tgctttttcc ggctttatca atcccaaccc aatgccatta aaccgatcgg
    24841 aattattaac aaaaacagta taatctccca gggagttacc aatcttttct gcaagcttaa
    24901 aaaaacgata tacaatcgga ttttgattat cctgaaacga aaaaccgtgt gaggtatgaa
    24961 taattaaagg gattttacaa atgcgggcag ctaatctacc taaaaaacca ggcttggaac
    25021 cattagtatg cacaatatca aaactatgct ttttgaatat atagagtaaa tgaacaaagg
    25081 ttagcaaatc ccatagacaa atctgatggc gaaaagtagg aagcggaata taattataac
    25141 cccgatcctt tacttcctga acaaattctc caccgggttt gctggcaacc caaatttcaa
    25201 attcatcttt gcttaaactt tctaagaggt gcaaagaaaa cctctgcgca ccatacataa
    25261 ctggcaacat ctgtaggtgt aatacctttt tcatctctgt ccttatatca ttttttatct
    25321 gtgcaaactc tgcaatcttt gagaggtttt ttttctctcc gcccttcgcc cttcgctctc
    25381 cgctctccgc tctccgccct ccgccctccg cccttcgctc tccgctaata tctgtcgttc
    25441 ctacggaact aatttttaca tatcttcata tcgccgggtt aaaaacccgt cgttaatatc
    25501 tgtcgttcct acggaactnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta
    25621 atatctgtcg ttcctacgga actttctatt ctaaatcctt taaatcccta aaatcctgtt
    25681 catcccatac ctattatcaa ttttatctgt gtaatctgtg taatctgtga gagacattat
    25741 ctttttaaat ctgcgtcatc tgcgaaatct gcgtgagccc tttttctctc cgctctccgc
    25801 cctctgctct ccgctctccg caaatcacaa tcctgttcat cccctaccta ttcttttttt
    25861 atctgtgtaa tctgtgtaat ctgtgagagg ttttttcatc ttccctccag tacgatcacc
    25921 gctatagaat ccgctttgat attcgtaaac tgtatctggt ttcctttcac ttttgccggc
    25981 atttccacca cttctttaat tttcggattt ccatgcaagc acagtgtaaa actctccaaa
    26041 tcatctggaa acatcaatcc gatctgatct tcattttcat ttatcgtaac tctttttcgc
    26101 ttttcccacc agtcaactaa ttcccctgcc gtagtcagat aggtattact acttttaatt
    26161 agcgccaata gataaggata gagtttattc atatagctga tattgctgaa actggcaatc
    26221 gtgaaatctg tgctgaatat actatgtgtt cgcaatgcgt tttggaagaa ctttttcagt
    26281 tgatgtttag catcttccaa ttgcagatag ttatgtttat taatctttaa atattcatct
    26341 ctatatatgg taggaatttc ccaaaaatca gctttggagc catttatcca aggttgccag
    26401 ggtaaggaca ttccattttt gtatccagga agtgactgat atgcagtgca ttgactaaac
    26461 gccggagata ttttattatg caaatctctg attgtattat taattttata gcccagctgc
    26521 cggattccca atttttcttt acccaacaga tgcaacaaga tttgtttgcg cgttaagaac
    26581 tcatcctggg taagtttatc ggcagtagcc aaaagaccta tctcatttcc gttatgcaag
    26641 attttctgaa tttcttcctg cagatcagga tcttccagag agtaatcaat atcttcattt
    26701 gcctcggttg ctaaataaaa ggtactgcga cagttggagt ccttttctaa cttcaaaaat
    26761 tcatcgaaat tccagttaag ctcgtagtta gtaaatagat attgaaattt acctttaacc
    26821 gtatgcgcca gttgtttcca acgaaaagta aatagcattg ctaaatcgtc aggaagggaa
    26881 agtaccagcg aggacgcatc ccatttttgc aaatcatcta tactatgtga taaaagggca
    26941 gctgcctgtt gagccattgg ccaatacatt ttttgggcaa tacagatatt tttggttcgg
    27001 cagtgttctt tgatcagcga atccaccagc cagagcaaat tatctacaat tggcgtttc
//
LOCUS       DAOSTH010000016        26473 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_2032_length_26473_cov_12.087062, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000016 DAOSTH010000000
VERSION     DAOSTH010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 26473)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26473)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26473
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2032_length_26473_cov_12.087062"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PLB65_03825"
     CDS             <1..180
                     /locus_tag="PLB65_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77687.1"
                     /translation="YFYPEIRAEIIKSVTEIDEENIRRIAEKHGMLSMRDSGIARMRE
                     GLTDMTEVLYVTAED"
     gene            182..607
                     /locus_tag="PLB65_03830"
     CDS             182..607
                     /locus_tag="PLB65_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77688.1"
                     /translation="MSMWEMVLALFAVVLFTSISLSYNQALWTQTDYLNNATLVVQAN
                     HICHSVLDEIDAKLFSKSYSFLNIVSMFRDSTNVVYYPHLKQSFNIKITAIDSDSLGF
                     SLPSPNPNSLFKTVTVTVSGPSALRHNISLKRLYTKTNM"
     gene            610..1194
                     /locus_tag="PLB65_03835"
     CDS             610..1194
                     /locus_tag="PLB65_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77689.1"
                     /translation="MGTLLDVIGSVVIGGALLMMLFTFTAQLQETSQRSLYTATMVDH
                     MDQAAIKINNLFSLAGIGLSADSTCIVADSNKVTFRTYWDYNNNVLTDSPHSIQLKLD
                     TVTHPILGTAIIITQDGTQLTDLGYILYIEYLKFIYYNKWDQVTTNPSAVRSVDVLLT
                     FRRDAPSLVNRPLRTNIQLRCFFMNSYLRMGHSV"
     gene            1227..2468
                     /locus_tag="PLB65_03840"
     CDS             1227..2468
                     /locus_tag="PLB65_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77690.1"
                     /translation="MTTVFAGTLLTLYNHMVEFPSVMVRNQLYLETENASDFTLRNAI
                     RNANSIEFLKKFLGSDFSFEDNPTFTQVFNNYHIGTCRVDSVCYSFYNSPTNFMVKCY
                     LTGSLHGQKVSKTAEMAFNYPLSALGKKPNVIYLEMEQLLLFPWLYELLGSSNDKLPD
                     TSGNDYTGIFEGFSLISSTVPWGGAFSRYCSKFNGVDNYIQVAPKATNDSINTDSSFS
                     LMCFAKMDKQGRYGHETEMGTLMWVPSNPSDATLRNKPSAGIWFVPNSSSSASGTMHF
                     AVTQNDASKTMLEVTAPYTRTADIFWFLLGFPIYTGHLEYEWNSFGLTYNNGVLKGYI
                     NGALVGTSAGTNVRAYPSTYGMSIGRKDLRGGSFSSSDYRYFCGVIDQIGMEDKALAP
                     SEMLSWHNGVLSPAKIFYVKD"
     gene            2474..3022
                     /locus_tag="PLB65_03845"
     CDS             2474..3022
                     /locus_tag="PLB65_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77691.1"
                     /translation="MHYSLLLLVSGLRTYEYYLPWLLPILVLLIFILFFFKQSRKITK
                     QQKWINELEQQTTHIEELQNRSQELDDLLVKYETQKKELDNALAKLEEWDKLVPELKE
                     IARLDHDINTPLCVVTLSLGRAQKVGKEQNDEALLTMVDDIFEAVNRIGEIMQKVAVL
                     KKSPLISYKSGGQELSEERGTV"
     gene            3019..3885
                     /locus_tag="PLB65_03850"
     CDS             3019..3885
                     /locus_tag="PLB65_03850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ77692.1"
                     /translation="MSVKKILVVDDEQNVRDLFRAFLDDMGYSVTTAIDGIDALDKIA
                     NERFDLYIVDIYMPRMGGLELISRLKELQPSAVIIVTTGYSGLDVDFRSIRNAAFMYL
                     SKPIKPDELIRAVEAGLAHSAEPSKSSTSMIMEEPVYTDKMPEMLLLHGFTLEQELQF
                     KSMGITTEYKAGESITMDREDGSMIFVERGYVNVYYNTDLVDTLKEGDIWGEETFVNP
                     TALFTTLKASSDAIIRYFPRKKIIEFFTYSDKSLTDRYTINLIQCMYIKWRRSIVRLA
                     LATRTSPIERNI"
     gene            3895..5103
                     /locus_tag="PLB65_03855"
     CDS             3895..5103
                     /locus_tag="PLB65_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOQ77693.1"
                     /translation="MRLSFSEALQREIPENYQGVEQCDIINQWLTHHPDQESECRDLL
                     KLILMQAKDLSASDVDLGAPGCNNKIWMRIYGNKKPVEDLGVFSCTETNSLILSWLTP
                     SQRLRLFTQKSLDFPISFYINGKETRFRGTAFFDRNYLGANFRRINDFLYSMEDLGIP
                     EPIANRMNLKYEKTGLILITGITGSGKSTTLDAIIDMNNRNNAAAIIAIESPIEYVHH
                     SKQSLVRHREVGIDVLSFEHGAIEALRQDPDIIVVGEMRDPQTISTVLEITDSGHKAF
                     TTLHTSSAIDSIHRIIAEFPPDSQERVRNRLADVLSVTMSQKLVPTVDGKLALAKEIL
                     SVDSSIQAAIRNKNIGEIYQMMVEGRRTGMCTLEQDLKELFRKGIISQQTAINFANNK
                     KRMQQLLTML"
     gene            5127..7313
                     /locus_tag="PLB65_03860"
     CDS             5127..7313
                     /locus_tag="PLB65_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77694.1"
                     /translation="MKRKPKEAFGIVQDGLTIRIVHLRKDESDTFLMGLDSIELESDW
                     YKADQVIAGSPNADFIPTEIQNLDINQYDVYETATQEERESTLPTTNAKPTTVLLSKF
                     NFQNGVIALNIHEEHIIKDTSGKVDKKDIVKFRKEKLTKLQMKSGEWSSCIVEAAGQK
                     QHWLYTGPNMLLHTLQDYATEAHTKLYYQLADANDLILTDFFRYTQNIEEGKTVLLVY
                     LGIEYRKMFLFQDGKWINTFPLQITQSRPEPEVINSKIALALDSAQIAEPDEIVLTGD
                     WASRELCDYMNTQNISVQVKLLHFPNLTIAHTGDNELNEEVISPYALAIAVAYKALNY
                     DNPVFSKCNFLPAKLLEEQKELKVVWHGFIVLTLIFGVVMYSTIHYLTLNRSISNYKR
                     ANQEMEFTFNRLKAENAILEQIRSEIESYKENINRIGTILQGKNRWTEMFNVLNTGFA
                     SQPSSWISNLKKADKEDRIVITGTTTRRENIAKIANSLPNSRISKVTNADIRKKKVWN
                     FEMDFDLPEVDWVHDIENEFIQENPEVVERIIAKPKSKSKSVRTSVKPVITNGTLSKA
                     VSTKSNGIGINYNYGVLPLINSDYLPQPIGNELANNRKLASEYQNFLSSINKVNMLEY
                     RFLGQKILAKYEDSSILPLIRWWIAYRLYCDKDYRTASMYLEPNLRTRDYYYPYSVLL
                     QARIDFASENDRFQQLYDSIISQYPDSDVYKQASIDLKYIREGVLR"
     gene            7310..8179
                     /locus_tag="PLB65_03865"
     CDS             7310..8179
                     /locus_tag="PLB65_03865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77695.1"
                     /translation="MNNTARNTLVLASLLIVTGAVSYAISGRKHSKLNELKTKNKELT
                     QQIANIKNMMENKEELEQERELQIALASQRSKAIIDIDTPTITYDYLLSLLRWMNKDI
                     IYDFALSGKADSKEASQGTYNEYVLSGKCNYLDFVDFTRNLEYQRAVITVEDISLAYT
                     AASNDTIDFSMIFRTHYQQGGVPISELSAKKIDKYVTAYQLFRPRVYETPIDYDFDTR
                     LLDIDNSTLIAITGSKAFIRDSKGLIRILSPGDRVLWGYLYQVYSKEGKAIFRVNKYG
                     YEEDQILTISKER"
     gene            8191..9339
                     /locus_tag="PLB65_03870"
     CDS             8191..9339
                     /locus_tag="PLB65_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77696.1"
                     /translation="MKKSIILVCLILAISITGLLAQTQKEMEKLTLSKDIRINEALQI
                     LEPFFIRDSGKKLINLSSYNGPLGVPINNLDYKEALELILLKNNLIQTEAVGYIAIQD
                     KAATDLGIGVETGKDSIMPTAEAVQLPEGVSPKDKQVLINAVIMLVDKSYSKSLGIDW
                     STLVDGKVKVNAGFSGASQVTAPMNLSAETSTEAGNYTIDINTLLKTIETEQKGSILA
                     KPNIRVSNARKGHIQVGQDISIKRVDEAGNTVESFFSTGVIMDVEPTIIKVDDEEVIY
                     LKLNIERSSGVPSDVSTIITKSTSSTEVILFSNEEIAIAGMFDTDETTVRSGIPILKD
                     LPWWVFGIRYLTGYNTVEKKERELVITIKAEIVDNAIDRLLESRSTHM"
     gene            9363..11927
                     /locus_tag="PLB65_03875"
     CDS             9363..11927
                     /locus_tag="PLB65_03875"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ77697.1"
                     /translation="MKISQRLLLLFIVIAIVFGAFFYLFNYIKQEEMKVYIEYDNNQQ
                     RLALNALIATKSDQQMRFLDEFSINDDIYSAVASHNVTKAEKLLTPLISSFGYSVAQI
                     YDEQGHLLLNLYSESLPELNNLLIDSIVLDSLKTGKAMQYFHRLQHLVLENAISSIHS
                     SADTMREDKIAGYLLIAQVWDQKRLSEFTKLLDFNANISLSKPIASDEDKATQYRTTI
                     TFPLNDLNGYEIAWLTFTRSNPYLRQLRSLGNLILFGTLGFIFIFLLVHFILIHQWIS
                     SPLQLISNSLSDNDPEYIKPLLDKNNEFAAIALLIQNFFVQKDQLIQEIEERIRTEAK
                     LKEAEEQTRKILLTSPEAIIVTDIRGKVLSINAEGLKILGVENEESVLFNPISAKDFV
                     ARWELRALYQMLAKLRKGIPVKNLEITFDNGKGQSFPSLVSASVITDNEGKPNRFIFI
                     ARDITDLKNLERQLRQSQKMESIGTLAGGIAHDFNNIITIIAGYIALARGKISDPQAS
                     EADLNQAIKACLRAKSLIGKILTFARQGELEVEQVSVKEVLEDALPMIRATLPSNINI
                     ETNIECERFVMADVTELQQVLINLASNAFHAMRPDGGTLTIKLNEISGFELIGIDPHI
                     ELERDYLHLEVSDTGFGIAPEIINRIFDPYFSTKAAGEGTGLGLSIVHGIITSYKGFI
                     TVHSIPGEGTSFHIFLPIVNAPKEAKKEEQPKTVYPYIPAKLMVIDDEPDLADIFHQA
                     LTNAGYDVQSFNNSTVALEAFENNPDDFDLVIADVTMPQLDGMKLTAKIKALRNIPVI
                     LYTGFSDHGVQQRATEAKVDRLLSKPLLPDELVAEVRRILYLISGGDINNNNPDYS"
     gene            11991..12368
                     /gene="gcvH"
                     /locus_tag="PLB65_03880"
     CDS             11991..12368
                     /gene="gcvH"
                     /locus_tag="PLB65_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOQ77698.1"
                     /translation="MKIQDDLYYTETHEWVQVNGDIATIGISDFAQHELGDIVFVELP
                     EVGQKVSSGEPCGSIEAVKAVEDLVSPISGKVQEKNTDLEDSPELINKSPYEEGWLFK
                     VKISNQEELDNLLTAAEYQRMLP"
     gene            12397..13023
                     /gene="gmk"
                     /locus_tag="PLB65_03885"
     CDS             12397..13023
                     /gene="gmk"
                     /locus_tag="PLB65_03885"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOQ77699.1"
                     /translation="MIARNSSFLIILSAPSGGGKSTLVNEVLKVAKNIEYSVSYTTRS
                     PRGKEENGVHYHFVSEAEFLKRIDEGDFLEHARVFGNWYGTSKSFIKSRLAKNHHVIL
                     DIDVQGASLISATDIPYVKIFILPPSMQVLKKRLMLRATDSEEEIAKRIQIAKEEVTH
                     IPKYDYLVINDELEKAVQDVLAIIRAEENRVCRYSHPCEDFINIGENQ"
     gene            13020..13439
                     /locus_tag="PLB65_03890"
     CDS             13020..13439
                     /locus_tag="PLB65_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77700.1"
                     /translation="MIHEKIETFLEKADINYLFCLLSELEAYRLLQLPVYVRQKFPEK
                     ITVMAMNHVAENEVPDYISELAEAELAAMEQESPFIEEEGGEEEAEETVDDSTKFIED
                     FADSEGFKEEEDEDEDEEDEDEEDFYDYDNDSEDDDL"
     gene            13443..14174
                     /locus_tag="PLB65_03895"
     CDS             13443..14174
                     /locus_tag="PLB65_03895"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOQ77701.1"
                     /translation="MKLNALHQYLEFLANSGIKELYFPGSDKRQRLNDLQIQYSTCSK
                     CPLHKNHHNFVYGEGNPDAIAMLIGEGPGEQEDLKGRPFVGAAGQLLDKMLAAIHINR
                     SEIYITNIVKCRPPGNRNPELEERLACLPYLVEQINIIQPKILLIMGLVAAQTLLGNN
                     NSLGWHREKIHYFMDIPVFVTYHPSALLRNSNWKRPAWIDLQEFEKEYRKILNEQTNF
                     DAKFPDKPNIATSNALTDSELPLSD"
     gene            14191..15015
                     /locus_tag="PLB65_03900"
     CDS             14191..15015
                     /locus_tag="PLB65_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ77702.1"
                     /translation="MCNSKIFPSSVFQRSLLLSVLFSLFLLVFSCLQARIAIYGDTRS
                     NPDIHKQIVKQIATFKPEAIFHTGDLNSSGKNQKEYDTFFQIISPLTQFGEFYPARGN
                     HEKDLSLFLKNFPYLNGSSYYTVMYDSIRFIILDSVLDLSPTSEQYCWLHEQLQEKIP
                     SLLILHHPVFSSGEHSDELGLQLFLPELLQQSSVKVVFSAHDHNYERSEYNNITYIVT
                     GGGGAPLREIYSHNPYSLVFSKQHHYIIAEREGPLINFKVYALSGDIIDTFLIGGF"
     gene            15018..15869
                     /locus_tag="PLB65_03905"
     CDS             15018..15869
                     /locus_tag="PLB65_03905"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine kinase"
                     /protein_id="HOQ77703.1"
                     /translation="MPNPNVLAIHDLSGFGHTSLMAVIPIMYRMGIEVATMPSALLSA
                     NTDHPGYIIQDNSDLLEKSFQHWKELKLSFDAIYTGFLGSPKQVALLLDYLPKLKKNE
                     TITLVDPVLADEGKLYSCYTWEMVEAMRSLLAISNLITPNWTEFAFLSGYQTWEAIKP
                     EQTKERCRKLAAWGPRYIVVTSAPNSKGKFSSIVFYDADNDYYQELDCLYAPVNFPGS
                     GDCFSAMLLAGIINGYSIINSIKGAIAFMHKAIEISIPTVVNRITGINLAAALKNDPF
                     LFFSDKA"
     gene            16211..19231
                     /locus_tag="PLB65_03910"
     CDS             16211..19231
                     /locus_tag="PLB65_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOQ77704.1"
                     /translation="MKKLAIVLGLALLVTGLFATFEWSDAVAIRQGVNIEWFRTGIET
                     TDGGAIYVWSDTKLGERDLWAQKVNASGDMVWGEPILIDGKLDRQEDPVITRTSDNNY
                     VIAWVDFSADLDGDIYAQKIDEQGNLLWQVGGVSVCSLVGMQISINMEPDNEGGVFII
                     WVDSRHPSKDLFGQHLSASGNALWTQNGIPIADGLGDEIQNTMLPDGQGGFIIAYTHS
                     YASNDDLYAKHFDANGTMTWPNPVVISNANGNQSDIRMAALTDGDFVVTWQDKRNTDP
                     DIYAQKINLAGDLLWGDYLVVYSDQDGLARPQLSPRIVKTSDNCAIIVWEDFSLDTQN
                     ADLFAQKISTTGTKLWSEEGVALCTAEFAQYGPRMAPDDNGGCYVVWDDLRNGNAPND
                     DIYAQHLSSTGVALWEANGKPICTAPNQQNGSLIKVSNNTVFINWMDIRNGSVGIYYQ
                     SLSSTGTPLLATNGVEVFWGLSGDTPLDNYLILKRSSDVVIIWQDTRFANDGYRIYFQ
                     FLNPDGSIGLETNGRPVTTSVGGTQSTPCAYVTPDDQIVIIWEDARGDNPKVYAQLIG
                     QNGERLWGDEGIPLTVSSPIRQKDPKVTYYNGSFYFGWSGSDQIGLNFFYHVYGQRIQ
                     NGQKMWGPDGLMISNLSQSDFNNECTLYDLIDNYYVWERYHPDNTRTVWVKRVAEDGS
                     SMDNWPEDGLRASTYNDYDTIQLLPKAHKTPDGIFVMWKDARGDFLQNYWGQHISENG
                     NYLWNTIGVNLADSGREQELPTITVTDSGITFAWCEIIDGVNDIKAQKFSFTGSPLWG
                     DTGFFVVQRDSTQSTPFLASFPNGGYLISWEDYNIERDLYYKYIRENGDMIGNINGNV
                     LCDKAKSQYQPQIAILNNKAYCVWADGRSSGKTQILGLYAQKLGNGTPNIDEIAPSVQ
                     NMMLQQNYPNPFNPQTTINFTLKDNIHSLNLSIYNIKGQLVKTLYRGALQKGQYNLVW
                     DGTNDSGKQVSSGIYFYKLSNGKETQQKKMVLMK"
     gene            complement(19625..20359)
                     /locus_tag="PLB65_03915"
     CDS             complement(19625..20359)
                     /locus_tag="PLB65_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HOQ77705.1"
                     /translation="MTKKNALHPLTHILMALLLSTMVFIVKKPSSLLFLTLLASIYAA
                     FRMKNGWQKILKTLWHTLPFLLVLSFFQIVFRRSGSLLWSYGILKISSEAVQISIQLA
                     LRLLTVIYCAKSLAVMEYPEFSKAFSAIRFPEELSFMLSYAIHLVPKFLAQLKGFVTG
                     LKLRGIDSANLSIAKRLQVYKQLAITSLAELIRNSETSAIALELRGFRSEGKRTKLYQ
                     QKFSYRDILFLLVFSGIVVLVTNLSW"
     gene            complement(20356..21705)
                     /locus_tag="PLB65_03920"
     CDS             complement(20356..21705)
                     /locus_tag="PLB65_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ77706.1"
                     /translation="MLILKDFGISFADRNLLEGINIEIPEGTVFHLKGPNASGKSSLL
                     NAISGIIPEYVQAKTTGDLIWNNIDLQTIPLKEKHHYLWHQLSDTEAQIFFPDCISEL
                     AFALENMAIPAEKIWERVNLATQQFGLSNLLYRDPSTLSGGEKKLLLCAIAETIDPPI
                     LLLDEPLNGLDNRAVKLVLKWITDKKEQGKIIVVVDHNPIIETLADFSFILADNPQLP
                     KTDIAFISNNYLNIADNLGYQINPNTDILYRVEELDFAYPQSKPLFTKLTVEIGSKQN
                     ILLTGNNGAGKSSFLKLLAGLIKPLKGKIYLGGKLMQGLDTKNFEYIYYQSQITKENL
                     LGISVAQNWLFWQLAIKQLPDLSLKDDPLFTELSAGQQKMVSQQILPQLLSKFWILDE
                     PFASLDKDAGTKLLQLLQYKSKKHPGMLIVSHSLEEHNDMFDRVLTIRNHFLQEETR"
     gene            complement(21705..22631)
                     /locus_tag="PLB65_03925"
     CDS             complement(21705..22631)
                     /locus_tag="PLB65_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77707.1"
                     /translation="MKKYICLLILLEVIVFLTALEIIDSKGISHIYDNAELYKLETQE
                     LKTTREKDGIVRLNNWQGFRFDIWLKQQKLGDFATIRFESSDRYLVTLEKSEFDSLES
                     YIVIAQDGIPFEEYMLRLIFPTLREMQWIKGLDRVVLENFHPLIRPTRFFLLQDTLKK
                     YTLQKDLKPFVNIEGYYLSDVLMDLSPAEEKKVILYSRDGLKQSLTYPLHLQGAVLEK
                     TPEDLYNLKSPQIPGGMWMKDLVYLQCDNYALISEDSINQLIRLAKILHWETTPEMQF
                     RIVYKDSEEIMNFTDALAEPQVFRGALYFEIF"
     gene            complement(22631..23167)
                     /locus_tag="PLB65_03930"
     CDS             complement(22631..23167)
                     /locus_tag="PLB65_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HOQ77708.1"
                     /translation="MKLLQHFTVKDLIIITSIAALGIAVKPIINPITKIISTPLGIPG
                     GSLTGGLYMLWLVLTIVIVDKPLTGTLYGFLQSILVLLIGMAGRMGAFSLVAYTLPGI
                     IADLIYWLYPKKNKLLTHLLLCAFANLTGSLVSSAIFFKMPPLMIGFNTCLALASGII
                     GGYLGFGIYQSLKATRII"
     gene            complement(23167..23640)
                     /locus_tag="PLB65_03935"
     CDS             complement(23167..23640)
                     /locus_tag="PLB65_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HOQ77709.1"
                     /translation="MERPSWQQYFMEMAYLAAKRSTCLRRKVGAVLVRDNQIISTGYN
                     GSPKGVPHCAEIGCLRKQQNVPSGKNHELCRGVHAEQNAIIQAAINGSSTRGSILYCT
                     NQPCSICARLIINAEIKTVYIAETYPDELGEELFREAGIELIQYELKSNTLKKLI"
     gene            24213..>26473
                     /gene="polA"
                     /locus_tag="PLB65_03940"
     CDS             24213..>26473
                     /gene="polA"
                     /locus_tag="PLB65_03940"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HOQ77710.1"
                     /translation="MADKLYLIDGTALLYRAHFAFIKNPLINSKGKNTSAIFGVINSF
                     LHIIENTQAAYVAISFDRKAPTFRHTLYEAYKAQRPPMPEELTAQIEPVKKFFELIGL
                     KEIGMDGYEADDILGTMAKQFKDQFQIVFLTSDKDYCQLIDERSVMLDPMKDVILDRD
                     AVYAKYGVYPEQFIDYLALVGDPSDNIPGVKSIGPKTASMLLSEYKTLDNIYAHLDFI
                     KPRWKELLLENKENAYLSKELATIKTDVPIPKPDLEYLTFWTKDLKKAIPLLEEYEIN
                     SIKRKIELNYPEPTDTIQQSEYQDDIFNMESKKSEAVSEADTKMPFKAILVNTRNFVT
                     LLKEIKEAQLISLDTETDSPDPMLANLAGISFCMNEAKAYYLPLGHQMADNLSLPEVI
                     RHLEDAIQGKLLLGHNLKFDLIVLCRHGLNLNNPIFDTMLAAYILDPGTLNYSLDACA
                     VSELNYKMIPISDLIGKGKNQSTFDYVELDRACIYSAEDAWVVYKLYPIYHRKVDFSV
                     MANLFYTIELPLIKVLQRMEMNGMAIDCSVLTEISHKINQELKKLTDIIYAYAGYEFN
                     LNSTQQLAKMLFEEKKLPTRKKTKTGYSTDNSVLEELAEDYEIAGTIIQYRQLAKLQS
                     TYINALPKMINPETGRIHSSFNQTVTSTGRLSSSNPNLQNIPVRTEIGREIRKAFYAK
                     DEEHLILDADYSQIELRLLALMSEDEVLIEAFKQDLDIHKRMAAKIYNVSLEQVTPDQ
                     RRAAKTINFGILYG"
BASE COUNT         8615 a         4717 c         5088 g         8053 t
ORIGIN      
        1 tatttttacc ccgaaataag agctgaaatc ataaaatccg tcaccgaaat cgatgaagaa
       61 aatatccgta gaattgcaga aaaacacggt atgctctcta tgcgcgatag tggtatagcc
      121 agaatgcgcg aaggattgac agatatgaca gaagtccttt atgtaaccgc ggaggattaa
      181 aatgagtatg tgggaaatgg ttttagcgtt attcgcagtg gttctattca cttcgatatc
      241 gcttagctat aatcaggcat tatggactca gacagactat ttgaataatg ccacccttgt
      301 ggttcaggca aaccatattt gccattctgt tttggatgaa atagatgcta aactcttctc
      361 caaaagctat agttttctta atatcgtttc tatgtttcga gattcaacca atgttgtcta
      421 ttatccccat cttaagcaga gtttcaatat caaaataaca gccatagata gtgattcttt
      481 gggattttca ctgccttctc ctaatcccaa tagtttattc aaaactgtaa ctgtaaccgt
      541 ttcaggacca tctgctttga gacataatat ttctttaaaa cgactttata caaaaacgaa
      601 tatgtaatta tgggaacttt attagatgtt attggcagtg ttgtaatagg cggagcatta
      661 cttatgatgc tatttacatt taccgctcag ttacaagaga cctctcaacg ctctttatac
      721 acagcaacta tggttgacca tatggatcag gcagccatta aaataaataa cttattttct
      781 cttgcaggaa taggtttatc tgcagattct acttgcatcg tagcagacag taataaggtt
      841 acttttagga catattggga ttataataat aatgtcctaa ccgattctcc ccattccata
      901 caacttaaat tggatactgt tactcaccct attcttggaa cagctataat tataactcag
      961 gacggaactc agctaactga tttggggtat attctgtata ttgaatactt gaagttcatc
     1021 tattataaca aatgggacca agtaactaca aatccttctg ctgtcagatc agtagatgtt
     1081 ttattaacct ttcgcagaga cgcacccagt ttagtaaacc gtcctctgcg aaccaatatt
     1141 caattaagat gtttctttat gaacagctat ttaagaatgg ggcatagtgt ataatgggaa
     1201 gatcatcttt acttgttatt attttaatga ctaccgtatt tgccggtact ttacttaccc
     1261 tttataatca tatggttgag ttcccatcgg tgatggttag aaaccagctc tatttggaaa
     1321 ctgaaaatgc cagtgatttt actttaagaa atgctattag aaatgctaat tcaattgagt
     1381 tcttgaaaaa gttcttaggc tcagatttca gctttgagga taatcccact tttactcagg
     1441 tatttaataa ctaccatatt ggtacttgcc gagtagatag cgtttgctat agcttttata
     1501 atagcccaac taattttatg gtaaaatgct atctcacagg ttcattacac ggacagaaag
     1561 tatccaaaac agcagagatg gcttttaatt atcctttaag cgccttgggg aaaaaaccaa
     1621 atgttatata tctggagatg gagcaattgc tacttttccc ctggctttat gagcttttag
     1681 gctcttccaa tgataaattg cccgatactt ccggaaatga ttacacaggt atatttgagg
     1741 gattttcttt gatcagttcc actgtccctt ggggtggagc tttcagccga tattgttcaa
     1801 aattcaacgg tgtagataac tatatacaag tagctcctaa agctactaat gacagtatca
     1861 ataccgatag ttctttttca ctgatgtgtt ttgccaaaat ggataaacag ggtcgctacg
     1921 gtcatgaaac agaaatgggg actttaatgt gggttcccag taacccgagt gatgctactt
     1981 taagaaataa accctctgct ggaatttggt ttgttcccaa tagcagttcc tccgcttcag
     2041 gaacaatgca ttttgccgtt acccaaaatg atgcttcaaa gacaatgctg gaagtgacgg
     2101 ctccctatac aagaactgct gatatttttt ggttcttgct cggttttcca atttatactg
     2161 gtcatttgga atatgaatgg aattctttcg ggcttactta taataacgga gtattgaaag
     2221 gttatataaa tggtgcgtta gttggaactt cagccggaac aaatgttaga gcgtatccca
     2281 gtacttatgg tatgagcata ggtagaaaag atttaagagg tggaagtttc agctccagtg
     2341 attacagata tttttgtggt gtaatagatc agataggtat ggaagataaa gctttagcac
     2401 ccagtgaaat gctaagttgg cataacgggg ttttatctcc agctaagatt ttttatgtaa
     2461 aggattaagg gttatgcatt acagtttact tttattggta tccggtctaa gaacttatga
     2521 gtattatctc ccttggttat tgcctatact tgttttactg atatttattt tatttttttt
     2581 taagcagtcc cgcaaaataa caaaacaaca aaaatggatc aatgaacttg aacagcagac
     2641 tacccatatt gaagaactac agaacagatc acaggaattg gatgatctct tagtaaaata
     2701 tgaaacacag aaaaaagaat tggataatgc ccttgccaaa ttggaagaat gggataagct
     2761 ggttccagag ctaaaagaaa tagcgcgttt ggatcatgat attaatactc cgctttgtgt
     2821 ggtaacatta tctttgggca gagctcaaaa agttgggaaa gagcaaaatg atgaagcatt
     2881 gcttactatg gtagatgata tttttgaggc agttaataga atcggtgaga ttatgcaaaa
     2941 agtagcggtc ttgaaaaaga gtcctttaat ttcctataaa tccggtggac aggagttaag
     3001 cgaagaaaga ggaacggtat gagcgtaaaa aaaatattag tcgttgatga cgaacagaat
     3061 gtgcgagatt tattccgtgc ttttttggat gatatgggtt attctgttac tacggcaatc
     3121 gatggaatcg atgctttaga taagatagct aatgaaagat tcgatctcta tatcgtagat
     3181 atctatatgc cacggatggg aggcttggaa cttatcagtc gattaaagga acttcaaccc
     3241 tcggcagtaa taatagtaac tacaggttat tcgggattgg atgtagattt ccgtagtatc
     3301 cgtaacgctg cttttatgta tctttccaaa cctattaaac cggatgaact aatcagagct
     3361 gtagaagcgg gtttggctca ttctgctgaa ccgagcaaat ccagtacttc gatgattatg
     3421 gaggaaccgg tctatacaga taaaatgccg gaaatgttac tattacacgg ttttacattg
     3481 gaacaagaac tacaattcaa gagtatggga attacaacag aatataaagc cggagaaagt
     3541 ataacaatgg atagagagga tggatcaatg atctttgtgg aacgaggcta tgtaaatgta
     3601 tattacaata cggatttggt agatacactt aaagaaggag atatctgggg agaagaaacc
     3661 ttcgtaaatc ccactgcact ttttactact ttgaaggcaa gtagcgatgc catcatccgt
     3721 tattttcccc gtaagaaaat tattgaattc ttcacttata gcgataagag tttaaccgat
     3781 cgctatacaa taaatcttat tcaatgtatg tatataaaat ggagaagaag cattgttcgt
     3841 ttagcgcttg caactcgtac atctccaatt gaacggaata tataaaggat aacaatgcgt
     3901 ttaagttttt cagaggcatt acaaagggaa atccctgaaa attatcaggg agtggaacag
     3961 tgtgatataa taaaccaatg gttaactcat catcctgatc aggaaagtga gtgtcgggat
     4021 ttattaaaac ttatcctgat gcaggcaaaa gatttatcgg catcagatgt ggatttggga
     4081 gctccaggtt gtaataataa aatctggatg agaatctatg gaaacaagaa gcccgttgaa
     4141 gatttaggtg ttttttcctg tacagagact aattcactta ttctcagctg gttaacccct
     4201 tctcagcgtt tacgtttatt tacgcaaaag agtttagatt ttcctatctc tttttatatt
     4261 aacggcaaag aaacccgttt tagaggaaca gctttcttcg atcgtaatta tttgggagcc
     4321 aactttcgga ggataaacga ttttttatat agtatggagg atttgggaat tccagagccg
     4381 attgctaacc ggatgaacct gaaatatgaa aagacaggat tgattctgat aactggaata
     4441 acaggcagtg gtaaatccac tactttggat gctataatcg atatgaataa taggaataat
     4501 gctgctgcga taattgctat cgaatcccct attgaatatg ttcaccattc taaacagagt
     4561 ttggttcgcc atagagaagt ggggattgat gttctttctt ttgaacatgg agctatagaa
     4621 gcattacgac aagatccaga tattatagta gtaggcgaaa tgcgcgatcc ccaaactata
     4681 tccactgttt tggaaataac ggatagtggc cataaagcat ttacaacttt acataccagt
     4741 tccgctatag atagtattca tagaataatt gcagaattcc ctcctgattc acaggagcgg
     4801 gttagaaacc gtttagccga tgtgctaagc gttactatga gccagaaact tgttcctact
     4861 gtagatggta aattggcttt agcaaaggaa attctttctg tagattccag tatccaagcc
     4921 gctattcgca ataaaaatat tggtgagatc tatcaaatga tggtggaagg acgcagaaca
     4981 ggaatgtgta ctctcgagca ggatttgaag gagctcttcc gcaagggaat aattagtcag
     5041 caaacagcta taaattttgc caataataag aagcgaatgc aacagctttt aacgatgtta
     5101 taattttgtt actttaagga aatgatatga aaaggaaacc caaagaagca ttcggaatag
     5161 tccaggatgg tttaactatc cgaattgtgc atctgcgcaa ggatgaatct gatactttcc
     5221 tgatgggatt ggacagtatt gaattagaat ctgattggta taaagccgat caggtaattg
     5281 ccggaagccc aaatgctgat ttcattccaa ccgagattca gaatcttgat attaatcaat
     5341 atgatgttta tgaaactgca acccaagaag aaagggaatc tactcttcct actactaatg
     5401 caaaacccac tacagtcttg ttatccaaat tcaatttcca aaatggggtt attgccctaa
     5461 atatccatga agaacatatt ataaaagaca cttccggaaa agtcgataag aaggatatag
     5521 taaaattccg taaagagaaa ttaacaaaat tacagatgaa atccggagaa tggagttcct
     5581 gcattgttga ggcagcaggc caaaaacaac attggttata tacaggccca aatatgttgt
     5641 tgcatacgct tcaggattat gctacagaag cacatacaaa attatattat cagttagccg
     5701 atgctaacga ccttattcta actgatttct ttcgctatac tcaaaatatt gaagaaggca
     5761 aaaccgtctt acttgtatat ttgggaattg aataccgaaa aatgttcttg ttccaggacg
     5821 ggaaatggat taacaccttc cccttacaaa taactcagag tagacccgaa ccggaagtaa
     5881 tcaattccaa gatagccttg gctttggata gcgcgcaaat tgccgaacct gatgaaattg
     5941 ttctaaccgg agattgggca agtagggaac tctgtgatta tatgaacacg caaaacataa
     6001 gcgtacaggt aaaattattg catttcccca atctaacgat agctcatacg ggcgataatg
     6061 aattaaatga agaagttata tcaccctatg ctttagcaat agctgttgct tacaaagctc
     6121 taaattatga taaccccgtc tttagtaaat gcaattttct acctgcaaaa ctattggaag
     6181 aacaaaagga gctgaaggtt gtttggcatg gttttattgt tctaactctt attttcgggg
     6241 tggtaatgta ctccactatt cattacctta cgctaaaccg aagcatcagc aactataaaa
     6301 gagctaatca ggaaatggaa tttactttta accgcctgaa agcagagaat gcaattttgg
     6361 aacaaataag atcagaaatt gaaagctaca aggaaaacat aaaccgtatc ggaactattc
     6421 tgcagggaaa gaatcgctgg acagaaatgt tcaatgttct caataccggt tttgccagcc
     6481 aaccttcaag ctggatatca aatttgaaaa aagccgataa ggaagataga attgtaatta
     6541 ctggcactac tactcgacga gaaaatatag caaagattgc taattcgtta cctaattcca
     6601 ggatcagtaa agttaccaat gccgatatca gaaagaagaa ggtttggaat ttcgagatgg
     6661 attttgatct tccggaagtg gattgggtgc acgatattga aaatgaattt attcaggaaa
     6721 acccagaagt tgtagaaaga ataatagcca agcccaaatc caaatccaaa tccgttagaa
     6781 catctgtaaa accggttatc acaaatggca cactaagcaa agcggtatca acaaaatcga
     6841 acggcatagg aatcaactat aattatggtg ttttaccgct tattaattcg gattatctac
     6901 cacaacctat tggaaatgag cttgccaata atcgtaagtt agcaagtgaa tatcagaatt
     6961 tcttaagctc catcaataaa gtaaatatgt tggaataccg tttcttggga cagaagatct
     7021 tagctaagta cgaagatagt tctatactac ctttaattcg ctggtggatt gcttatcgcc
     7081 tctattgtga taaggattat agaactgctt caatgtatct ggaaccaaat cttcgaacca
     7141 gagattatta ctatccttac agtgtattgt tgcaagcacg cattgatttc gctagcgaaa
     7201 atgatcgctt tcaacaactt tatgattcca ttatttccca atatccagat tcagatgtat
     7261 ataagcaggc aagtatagat ctcaaataca tccgtgaagg agtgttaaga tgaataatac
     7321 tgcacgcaac actttagttt tggcaagttt actaatcgtt acgggtgccg tttcttatgc
     7381 cataagtggc agaaaacata gcaagctgaa tgaactaaaa actaagaata aagaacttac
     7441 acaacagatt gctaacataa aaaatatgat ggaaaataaa gaagagttgg aacaagagag
     7501 agagcttcaa atagctcttg ccagtcagcg cagtaaagca attatcgata tcgatactcc
     7561 aactataact tatgattatc tattaagttt gctgaggtgg atgaataaag atattatcta
     7621 tgattttgct ctctccggta aagctgatag caaggaagcc agccagggaa cttacaatga
     7681 atatgtcctt tctggaaagt gtaattatct ggattttgtg gattttactc gcaatttaga
     7741 atatcaaaga gctgtgatta ctgtggagga tatctcgctg gcttataccg ctgcaagtaa
     7801 tgatacgata gatttttcta tgatttttag aactcattac caacagggag gagttcctat
     7861 aagcgagcta agcgctaaaa agatagataa atatgtaact gcatatcaat tgtttaggcc
     7921 tcgcgtttat gaaacaccta ttgattatga ttttgacact cgtttactgg atatcgataa
     7981 tagcaccctt atcgctataa caggatcaaa ggcatttatt cgtgatagta aaggtctaat
     8041 tcgtatcctc agcccaggcg atagagtttt gtggggctat ttatatcaag tttatagtaa
     8101 agagggaaaa gcaattttca gagtaaataa atatggctat gaagaagatc agattttaac
     8161 aataagcaaa gaaagatgag gttagcccaa atgaagaaat ctattatatt agtttgccta
     8221 atactggcaa ttagcattac aggcctgctt gcacaaaccc aaaaagaaat ggaaaaatta
     8281 acattgagta aagatatccg cattaatgaa gcgttgcaga ttttggaacc tttttttatt
     8341 agggattctg ggaaaaagct aattaacctc tcttcctata atgggccctt aggtgttccc
     8401 attaataatc tggattataa agaagcgctt gagctaattt tgctgaaaaa caatcttatt
     8461 caaactgaag ctgttggtta tattgctatt caagataaag ctgcaaccga tttgggaatt
     8521 ggagttgaaa cgggaaaaga tagtataatg ccaactgctg aagctgttca attaccagag
     8581 ggagttagcc ccaaagataa acaggtctta attaatgccg ttataatgct ggtagataaa
     8641 agctattcca aatcattagg tattgactgg tcaaccctgg tagatggcaa agtaaaagta
     8701 aatgctggat tttcaggggc atcgcaagta accgctccaa tgaatctttc cgcagaaaca
     8761 tccactgaag ctggtaatta tactatagat ataaatactc tgctaaagac tatagaaacg
     8821 gaacaaaaag gaagcattct tgccaagcct aatatcagag tttccaatgc ccgaaaaggg
     8881 catatacaag ttggccagga tatttccatc aagcgagttg atgaagctgg aaatacagtg
     8941 gaatcttttt tctctactgg ggtaataatg gatgtggagc ctactattat caaagtggat
     9001 gatgaagaag taatttacct gaaacttaat attgaacgct ccagtggagt tcctagtgat
     9061 gtttccacca tcattaccaa aagcacttcc tctacagaag taatattgtt cagcaatgaa
     9121 gaaattgcca tagctggtat gtttgatact gacgaaacaa cagtccgcag tggcattccc
     9181 atcttgaaag acctcccttg gtgggttttt ggcatccgct atctaactgg ctataacaca
     9241 gttgagaaaa aagaaagaga gctagtcata accatcaaag ctgagattgt agataatgct
     9301 atagacaggt tactggaatc gcgatctaca catatgtaaa atgataatat aaggaagatt
     9361 cagtgaaaat atctcaacgt ttattactgc tttttattgt aattgctata gtttttggag
     9421 cattcttcta tctgttcaat tatatcaaac aggaagaaat gaaggtttat attgaatatg
     9481 ataacaatca acaacgcctt gctcttaatg ccctaattgc tacaaaaagt gatcagcaga
     9541 tgcggttttt agatgaattt agcataaatg acgatatcta ttctgctgtt gcttcgcaca
     9601 atgtaacaaa agcagaaaaa ttacttactc ctctaatcag ctcctttggt tatagtgtag
     9661 ctcaaatcta cgacgagcag gggcatcttt tattgaatct atatagcgaa tcacttcccg
     9721 aattaaacaa tttgctgata gattccattg tgcttgattc tctcaaaaca ggtaaagcaa
     9781 tgcagtattt tcaccggttg cagcatttag ttttagaaaa cgctatcagt agtattcata
     9841 gttctgctga cacaatgaga gaagataaga tagcaggata tcttcttatt gctcaggttt
     9901 gggatcaaaa gagattaagt gaatttacaa agctgctcga ttttaatgca aacataagct
     9961 taagcaaacc tattgcttct gatgaggata aagcaactca atacagaact actattacct
    10021 tccctttgaa tgatctgaac ggatatgaaa tcgcctggtt aactttcacg cgttccaatc
    10081 cttacctaag acaattgcgc tctttgggaa atttgatcct ctttggaact ttgggattta
    10141 tctttatttt cttattggtt catttcatcc ttattcatca gtggatcagt tctcctttac
    10201 aattgatatc caattcttta agcgataatg atcctgaata tattaagccc ctgttagata
    10261 aaaacaatga attcgcggct atagcgttat tgatacagaa tttcttcgtt cagaaggatc
    10321 aacttatcca agaaattgaa gaacgcattc gcacagaagc caaacttaaa gaagcagaag
    10381 aacaaaccag aaaaatactg ttgacctcac ctgaggccat aatcgttaca gatataagag
    10441 gcaaggtttt aagcattaat gccgaagggc tgaaaattct tggcgtcgaa aatgaagaga
    10501 gtgttttgtt taatcctata tctgctaagg actttgttgc gcgttgggaa ttacgtgctt
    10561 tataccaaat gctggcaaaa ttacgcaaag ggattccggt taaaaatttg gaaataactt
    10621 tcgataacgg aaaggggcaa agtttcccca gtttagtaag cgcttctgta attacagaca
    10681 atgaaggaaa acccaataga tttattttta tcgctcgaga tatcacagac ctaaaaaacc
    10741 tggaacgcca attacggcaa tcacagaaga tggaatctat cggaactttg gctgggggca
    10801 tagctcatga tttcaataat attatcacaa ttattgctgg gtatattgct ttagccagag
    10861 gcaaaattag cgatccgcaa gcctctgaag ctgatctgaa tcaggcaatt aaagcctgcc
    10921 tgagagccaa aagtttgatt ggtaaaattc tcactttcgc ccgccaggga gaattagaag
    10981 tagaacaagt tagcgttaaa gaagttctgg aagatgcttt accgatgatt cgagctactc
    11041 taccttccaa tattaatatc gagacgaata tagagtgtga gcgctttgtt atggctgatg
    11101 taactgaatt gcaacaggtt cttatcaatc ttgccagcaa tgcttttcat gcaatgcgac
    11161 cagatggagg aactctaact attaagctaa atgagatatc aggatttgaa ctaattggaa
    11221 tagatcctca tatagaactg gaaagagatt atctgcattt ggaagttagc gatactggct
    11281 ttggcatcgc tcccgaaatt atcaatcgta ttttcgatcc ctattttagc actaaagcag
    11341 ctggagaagg aacaggttta ggcttatcca ttgtccatgg tatcataacc agctataaag
    11401 gttttatcac tgttcatagc attcctgggg aaggaaccag ttttcatatc ttccttccaa
    11461 ttgttaacgc tcctaaggaa gccaagaaag aggaacagcc aaaaactgtt tatccctata
    11521 ttccagcaaa attgatggtt atagatgacg aacctgatct tgcggatatt ttccatcaag
    11581 cattaactaa cgcaggatat gatgttcaat cctttaataa ctctactgta gcattggaag
    11641 ctttcgaaaa caatccggat gatttcgatc ttgtaatagc tgatgttact atgcctcaac
    11701 tggacggtat gaaattaacc gctaaaatca aagccctaag aaatatacct gtgatcctct
    11761 ataccggttt cagtgatcac ggagttcagc aacgcgctac agaggcaaaa gtagatagat
    11821 tgcttagtaa acctctttta ccagatgaac tggtagccga agttagaaga atcctatatc
    11881 ttattagtgg tggggatata aacaataata atcctgatta ttcttgacat aaaataacca
    11941 tccaaagatt tgggctatac aatatataat tctggtaaat ggaggaaaaa atgaaaatac
    12001 aggatgattt atattacact gagacccacg aatgggttca agttaatggc gatatcgcta
    12061 ctattggtat tagtgatttt gctcagcatg aacttggtga tattgttttt gtggaacttc
    12121 ccgaggttgg ccaaaaagtt tcttctggtg agccctgtgg ttccatagaa gccgtaaaag
    12181 cagttgaaga tctcgttagt cctattagtg gtaaagttca ggaaaagaat actgatttag
    12241 aagatagccc cgagctgatt aataaatccc cctatgaaga gggttggcta ttcaaagtaa
    12301 aaatatccaa tcaagaagaa ctggataatc ttcttactgc agcagaatat caaagaatgc
    12361 ttccctaaca gtacattctg cagtggttta aattctgtga tagccagaaa tagcagcttt
    12421 ttaattattc tctccgctcc ttcgggagga ggaaaaagca ctttagtaaa cgaagttctg
    12481 aaagttgcta agaatatcga atattctgtc tcttacacaa ctcgcagtcc ccgaggaaaa
    12541 gaagagaacg gagttcatta tcacttcgtc tctgaagctg agtttttaaa gcgaattgat
    12601 gagggagatt ttttagagca tgctcgcgta ttcggaaatt ggtatggaac ttccaagagt
    12661 tttattaaaa gccgcttagc taagaatcac catgttattt tggatattga tgtgcaggga
    12721 gcatctctta tcagcgctac cgatatcccc tatgtgaaga tctttatact tccaccttct
    12781 atgcaagtat tgaaaaaacg gttaatgctg cgggctacag atagcgaaga agaaatagct
    12841 aagcgtatac aaatcgctaa ggaagaagtt actcacattc ccaaatatga ttatctggtt
    12901 attaacgatg agctggaaaa ggcagttcaa gatgttttag ccattatccg agctgaagaa
    12961 aaccgggttt gccgttatag ccacccctgc gaggatttta ttaatatagg agaaaatcaa
    13021 tgatacacga aaaaatcgaa acttttctgg aaaaagctga tataaactat ttattctgtc
    13081 ttttatccga attggaagca tatcgtctgc ttcaattacc tgtctatgta aggcagaaat
    13141 ttccagaaaa aataaccgtt atggctatga atcatgttgc cgaaaatgaa gttccggatt
    13201 acatttcaga attggcagaa gcagaacttg ctgcaatgga acaagaaagt ccctttatag
    13261 aagaagaggg tggcgaagaa gaagctgaag aaactgtgga cgattctacc aagttcatag
    13321 aagattttgc cgattcagaa ggttttaaag aagaagaaga cgaagacgaa gacgaagaag
    13381 acgaagacga agaggacttt tacgattacg ataacgatag tgaagacgac gatctttaac
    13441 cgatgaagct aaatgcctta caccaatatt tagagttttt agcgaacagc ggtataaaag
    13501 aactctattt tccaggaagc gataaacgac aacgcttgaa tgatcttcaa attcaatatt
    13561 ctacctgtag taagtgcccc ttacataaaa accatcataa ctttgtttat ggagaaggaa
    13621 accccgatgc catagctatg ttgattggtg aaggacccgg agaacaagaa gacctgaaag
    13681 gaagaccttt tgtaggagct gcaggtcagc ttttggataa aatgctggct gcaatacata
    13741 ttaaccgtag cgagatttat ataactaaca ttgtaaaatg ccgtcctcca ggaaatcgta
    13801 atcctgaatt agaggaacgt ctggcctgtt taccctatct ggttgaacaa attaacatta
    13861 ttcaacccaa aatattgttg attatggggt tagtagcagc acaaactctt ttgggaaaca
    13921 acaattctct tggctggcat agagaaaaaa ttcactattt tatggatatt cctgtttttg
    13981 taacctatca tccttctgct ctcttaagaa actctaattg gaaaagacct gcctggatag
    14041 atttacagga attcgagaaa gagtatcgca agatcttaaa tgagcagact aatttcgatg
    14101 caaaatttcc tgataaaccg aatatcgcta cttccaatgc actcactgat agtgagttgc
    14161 cattatctga ttaagctaag ataataaata atgtgcaaca gcaaaatctt tccgtcttca
    14221 gtttttcagc ggagtttgct tttatctgtt ttattttcat tattcctcct ggtattttcc
    14281 tgcttacaag ctcgtattgc tatttatgga gatacaagaa gcaatcctga tattcataaa
    14341 cagatagtta aacaaattgc tacttttaaa ccggaagcta tttttcatac aggagatttg
    14401 aatagctctg gaaaaaatca gaaagaatat gatacttttt tccaaatcat ttcaccgcta
    14461 actcaatttg gtgaatttta tcctgccaga ggtaaccacg aaaaagatct ctctctattt
    14521 ttaaagaatt ttccctatct gaatggcagc tcatactata ctgttatgta tgatagcatc
    14581 cgttttatta ttttagatag tgttttagac ctctccccta cctcagaaca atattgttgg
    14641 ttacacgaac aattacagga aaagatccct tcgctgctga ttttgcatca tccggttttt
    14701 tcttctggag aacatagtga tgagcttggt ctccagcttt ttctccctga acttttacaa
    14761 caatcctcag tgaaagttgt ttttagcgct cacgatcaca attacgaacg ctctgaatat
    14821 aacaacataa cctatattgt tacaggtgga gggggagcac ctttacgcga gatttatagc
    14881 cacaatccct attcacttgt ttttagcaaa caacatcatt acattattgc cgagcgcgag
    14941 gggcccttaa ttaattttaa ggtttatgcc ttatccggcg atattattga tactttttta
    15001 attggtggat tttagatatg cccaatccta atgttcttgc tattcatgat ttatctggtt
    15061 ttggccatac ctctttaatg gctgttatac ctattatgta tagaatgggt atcgaagtgg
    15121 ctactatgcc aagtgctttg ctttctgcca atacagacca tccaggatat attatacaag
    15181 ataatagcga tcttttagaa aagtcctttc agcattggaa ggaactaaag cttagctttg
    15241 atgctatata tacaggattt ttaggttcgc cgaaacaagt agctctgttg cttgattatt
    15301 taccgaaact taaaaaaaat gagacgatta ctttggttga tcctgttttg gctgacgagg
    15361 ggaaattata cagttgttac acctgggaaa tggttgaagc aatgcgttca ttacttgcca
    15421 ttagtaatct catcactcct aattggacag aatttgcttt tttgagcggg tatcaaactt
    15481 gggaagctat taaacctgaa caaaccaaag aacgctgtag aaaattagct gcttggggac
    15541 ctcgttatat cgtggttact agtgcaccca attccaaagg caaattttcc tctattgttt
    15601 tctatgatgc agataatgat tattatcaag aattggactg cctttatgcc cctgttaatt
    15661 ttcctggttc cggagattgt ttttctgcaa tgcttttagc agggattatc aatggctata
    15721 gtattatcaa ttccatcaag ggagcaatcg cttttatgca taaggcaatt gaaataagca
    15781 ttcctacagt ggttaaccgc ataaccggta tcaatttggc tgcagcctta aaaaatgatc
    15841 ctttcctttt cttctccgat aaagcttaga aaagaaatat atcatattat accaagatgt
    15901 cttaatggag atcttggttt ttttttgatt atttatttta tgattaatga ggcttattca
    15961 gttctttaaa accttgtctc ctaaacaaaa tcaacatacc agagttgcat attagatatc
    16021 ctcttagtag ataataacta tttgtttgga aacaatcttg cattaatatt tatagatatg
    16081 gagataataa ttcgttttca caaatatgaa tggggttctg attatgcttt gctttatatt
    16141 ttatagaaaa ggacctaaat gtatagatgt tatggccaca taatgttata taagataagg
    16201 agatagagaa atgaaaaaat tggcgatagt gcttggtctt gcccttttag ttacagggct
    16261 ttttgccact tttgaatgga gcgatgcagt agctatccgt cagggtgtaa atattgaatg
    16321 gtttcgcacc ggtattgaaa ctaccgacgg aggagctatt tatgtttggt cagatacaaa
    16381 acttggcgag cgcgatcttt gggctcagaa agttaatgcc tcaggagata tggtttgggg
    16441 agaaccaatt ctaatagatg gcaagttaga ccgtcaagaa gaccctgtaa taaccagaac
    16501 aagtgataat aattatgtta ttgcctgggt agatttttct gcagatttag atggcgatat
    16561 ctatgctcaa aaaatagatg aacagggtaa tttactttgg caagtaggcg gagtttcagt
    16621 ttgttcctta gttggaatgc agatttcgat aaatatggaa cccgataatg agggaggagt
    16681 gtttattatt tgggtggaca gtcgccatcc cagtaaagac ctttttggcc agcatctttc
    16741 tgcaagtggc aatgctctat ggacgcaaaa tggtattcct atcgccgatg gactgggaga
    16801 tgaaattcaa aatacaatgc ttcctgatgg acagggagga tttattattg catacaccca
    16861 cagctatgcc agtaatgatg atctctatgc taaacatttt gatgccaatg gaacaatgac
    16921 ttggccaaat cctgttgtta ttagtaatgc gaatggaaac caatctgaca ttaggatggc
    16981 tgctttaact gatggcgatt ttgttgttac ctggcaggac aagagaaata ccgatcccga
    17041 tatttatgcc caaaaaatca acttagccgg agatttgctc tggggagatt atcttgttgt
    17101 ttatagtgat caggacggct tagcaaggcc gcaattgagt cccaggatag taaaaacctc
    17161 tgataactgt gctataatag tttgggaaga ttttagctta gatactcaaa atgctgatct
    17221 ctttgcccaa aaaatatcaa caaccggcac aaaactctgg tcagaagaag gtgttgccct
    17281 ttgtacagca gaatttgctc aatatggacc tcgtatggct cctgatgata atggtggttg
    17341 ttatgtagtt tgggatgatt tacgaaatgg taatgcaccc aatgatgata tatatgctca
    17401 acatctatct tcaactggag ttgccttatg ggaagcaaat ggaaagccta tttgcactgc
    17461 acccaatcag caaaacggaa gcttaataaa agttagtaat aacaccgttt ttatcaattg
    17521 gatggatatt cgcaacggca gtgtaggtat atactatcaa tcactatctt ctacgggaac
    17581 tcctctctta gctacaaatg gagttgaagt tttttggggt ttgagcggag acacacctct
    17641 ggataattac ttaattttaa agcgtagttc agatgtagta attatttggc aggatacccg
    17701 ttttgccaat gacggttata ggatatattt ccaattcctt aatccagacg gtagtatagg
    17761 tttggaaacg aatggacgcc cagtaacaac ttctgtggga ggaactcaat ccacaccttg
    17821 cgcttatgta actccagatg atcaaattgt tattatttgg gaagatgcca gaggtgataa
    17881 tccaaaagtt tatgcccagc tgatcggaca aaatggagaa agattatggg gagacgaagg
    17941 tattccttta actgtaagta gcccgataag acagaaggac cctaaagtaa cttattataa
    18001 cggttcgttt tactttggat ggtcaggttc agaccaaatt ggattaaatt tcttttacca
    18061 tgtttatggt cagcggatac aaaatggaca aaaaatgtgg ggaccagatg gattgatgat
    18121 ttccaatctc tctcagagtg atttcaataa cgaatgtact ctctatgatc ttattgataa
    18181 ctattatgtt tgggagagat atcaccccga taatactaga acagtttggg ttaaaagagt
    18241 tgcagaagat ggttcatcta tggataattg gcctgaagat ggattacgag cttctactta
    18301 taacgattat gatactatcc aattgcttcc caaagcccat aaaactccag acggtatctt
    18361 tgtgatgtgg aaagatgccc gaggtgattt tcttcaaaat tactggggac agcatatctc
    18421 ggaaaatgga aactatttat ggaatactat aggcgtaaat cttgctgata gcggaagaga
    18481 acaagaatta ccaacaatca cagtaaccga tagcggtatc acttttgcct ggtgtgaaat
    18541 aattgatggt gttaatgata ttaaggctca gaagttctct tttacaggat cacctttatg
    18601 gggcgataca ggtttctttg ttgtacaacg agattccact caatccacac catttttggc
    18661 tagtttcccc aatgggggtt accttatcag ctgggaagat tataacatcg aacgcgacct
    18721 ctactataaa tatattcgtg aaaatggcga tatgatcgga aacattaatg gcaatgtcct
    18781 ctgcgataaa gccaaatctc aatatcaacc tcaaatagcc attttgaata ataaagcata
    18841 ttgtgtttgg gcagatggac gatccagtgg aaaaactcaa attttgggac tctatgccca
    18901 aaaactaggt aacggaaccc ccaatatcga tgagattgct ccttctgttc aaaatatgat
    18961 gctacagcaa aattatccca atcccttcaa tccgcaaacc actattaact ttactttgaa
    19021 ggataatatc cactccctaa acttaagcat ctataatata aaaggacaat tggtcaaaac
    19081 tctttaccgc ggagccctgc aaaaaggcca atataactta gtgtgggatg gaaccaacga
    19141 ttccggaaaa caggttagca gcggaatata tttctataaa ctaagcaatg gaaaggaaac
    19201 acaacagaaa aaaatggtgc tgatgaaata ataaccgtga actgcatata aaaaatatat
    19261 gcaataagtg aatagcgtgt tagattaaca taggaaatta tattataaga gtccccattg
    19321 ccataattgg ttgttacata tagtacgaac tttgggcagt tgggactctt gttttgctct
    19381 tccccaatat ccacttttaa tgaactacga acttgggata ttacgcacca gagaacacag
    19441 atttgaattt tccttccggt gtaagacaga gcttggcaaa tcagcaagca atacgataca
    19501 gaagatttct ccagttatag aactcgttat tgataattgt gtaaatgata atgtagcaat
    19561 aatacacgag gtgtttttgt taagaagtac ttttatctat tattctgtct ctctatcact
    19621 tgggctacca ggaaagatta gtaactaata ctactatccc agaaaaaaca agtagaaata
    19681 atatatcccg atagctgaac ttttgttgat atagttttgt tcttttacct tcacttctaa
    19741 aacctctaag ttccaaagca atggcagaag tctcactatt tctgattagt tctgctaaag
    19801 aagtaattgc caattgttta taaacctgta agcgtttggc aattgaaaga ttggcagaat
    19861 caatgccccg cagtttcaat cctgttacaa atccttttaa ttgggctaaa aatttcggga
    19921 caagatgaat agcataagac agcataaaag aaagctcttc tggaaaacgg attgcagaaa
    19981 aagccttgga aaattctgga tattccatta cggctaatga tttagcacaa taaataactg
    20041 ttaataaacg taatgccagc tggatagaaa tttgaactgc ttctgaacta attttcaata
    20101 tcccataaga ccaaagcaaa gatccgcttc ttctaaaaac gatttggaaa aaacttaaga
    20161 ccaacaaaaa gggtaaagta tgccacaggg tttttagtat tttctgccaa ccatttttca
    20221 tcctaaaagc agcataaatt gacgccaaca aagttaaaaa aagtagcgag cttggctttt
    20281 taacgataaa taccattgta gatagcaata aagccataag aatatgtgta agtggatgca
    20341 gggcattctt ctttgtcatc ttgtttcttc ttgcaaaaaa tggttacgaa tagttaatac
    20401 cctatcaaac atatcgttat gctcttccaa cgaatgagat acaattaaca tcccagggtg
    20461 cttcttactt ttatattgca gcaattgtag aagtttagtt ccagcatcct tatctaaaga
    20521 agcaaagggt tcatccaata tccagaattt agatagtaac tgaggtaata tttgttgcga
    20581 taccattttt tgctgtccag cagatagttc tgtaaataat ggatcatctt ttagtgatag
    20641 atcgggtagc tgtttaatag ccagttgcca aaacagccaa ttttgtgcaa cactaattcc
    20701 caaaaggttt tctttggtga tctggctctg ataatagatg tattcaaagt tctttgtgtc
    20761 caatccctgc atcagctttc cacctaaata tattttacct tttaagggtt taattagccc
    20821 tgctaagagc ttcagaaaag agcttttccc tgctccatta ttaccggtta atagtatatt
    20881 ttgcttgctt cctatctcaa cagtaagctt tgtaaatagc ggcttcgatt gaggataagc
    20941 aaaatcaagc tcttctaccc ggtaaaggat atcagtgtta ggatttatct ggtaacctaa
    21001 attatcagct atattcaaat agttattact gatgaaggcg atatctgttt tagggagttg
    21061 cggattatct gctaaaataa aagaaaaatc ggctaaggtt tctattatag ggttgtgatc
    21121 taccaccaca attattttgc cttgttcttt cttgtcagta atccatttta agaccaattt
    21181 aactgcccga ttatctaatc catttagggg ctcatctaaa agcagtattg gtggatctat
    21241 tgtttcagca attgcacaaa gcaacagttt tttttcgcct ccgcttaagg ttgaaggatc
    21301 cctataaagt aaattactaa ggccaaattg ctgggtagca agatttactc tttcccatat
    21361 tttttccgcg gggatagcca tattttccaa agcaaaagca agttctgaaa tacaatcagg
    21421 aaaaaagatt tgagcttctg tatcgcttag ttgatgccat agatagtggt gtttttcttt
    21481 caggggaatt gtttgcaagt ctatattatt ccaaataaga tcaccggtcg ttttagcttg
    21541 aacatattcg ggaataattc cactgatagc atttagcaga gaactttttc cgctggcgtt
    21601 aggccctttt aaatgaaata cagtcccctc aggaatttct atattgatcc cttcaagcaa
    21661 gtttcggtct gcaaacgaaa tgccgaaatc ctttaagata agcatcaaaa aatctcgaag
    21721 taaagcgctc ccctaaaaac ttgaggttca gctaaagcat cggtaaaatt cattatttct
    21781 tcactatctt tatatacaat gcgaaattgc atctccggag tggtttccca atgcagaatt
    21841 tttgccagac gaattagttg atttatcgaa tcttcactaa ttagcgcata gttatcacat
    21901 tgtaaataaa caagatcttt catccacatt ccacccggaa tctgaggact ttttaagtta
    21961 tagagatcct ctggagtttt ttccaaaacg gctccctgca agtgtaatgg ataagttaaa
    22021 ctctgtttaa gtccatctcg actataaagt ataacctttt tttcttctgc tggcgataaa
    22081 tccattaaaa catcacttag ataataaccc tcaatattca cgaagggctt gaggtccttt
    22141 tgtaaagtgt atttttttag agtatcttgg agcaggaaaa aacgcgtcgg acgaataagc
    22201 ggatgaaaat tttccaatac aacacgatct aaacctttta tccattgcat ttctcgcaaa
    22261 gtagggaaaa taaggcgtag catatattcc tcaaaaggaa tgccatcttg agcaataaca
    22321 atgtagcttt ccagagaatc aaactcactt ttttctaaag ttaccagata gcggtctgag
    22381 gattcaaaac ggatagtggc aaaatcacct aacttctgct gttttagcca gatatcaaac
    22441 ctaaaacctt gccaattatt aaggcgaacg attccatcct tctcgcgagt agtttttagt
    22501 tcctgtgttt ccagtttata cagttctgca ttatcataaa tatggctaat acctttacta
    22561 tctatgattt ctaaagcagt taagaagaca ataacttcca gtaaaattaa aagacaaatg
    22621 tatttcttca tcatataatc cttgttgctt tcaatgattg ataaattcca aaaccaagat
    22681 agccacctat aattccagaa gctaatgcta aacaagtatt aaagccaatc attaaaggag
    22741 gcattttaaa aaagatagca gaagaaacta aagaccccgt taaattggca aaagcgcaca
    22801 acaacaaatg agtaagcagt ttatttttct taggatatag ccaataaatc aggtctgcta
    22861 taattcctgg aagtgtatat gccactaaag aaaaagcccc cattctaccc gccattccaa
    22921 tcaataaaac tagaattgat tgcaaaaacc cataaagtgt accagttaaa ggtttatcca
    22981 ctattacgat cgttaaaact aaccataaca tataaagtcc gcctgttagt gaaccaccag
    23041 gaattcctaa aggagtggaa ataattttag tgataggatt aataataggt tttacggcaa
    23101 ttcccaaagc agcgatggaa gtgataataa ttaaatcctt tacggtaaaa tgctggagca
    23161 gtttcattag ataagtttct ttagtgtatt actttttaat tcatattgaa tcagttcaat
    23221 tcctgcctcg cgaaaaagct cttctcctaa ttcgtcagga taagtctctg ctatataaac
    23281 agtcttaatt tcagcgttga taattaaacg agcacaaata ctacagggtt gatttgtaca
    23341 ataaagaatt gaaccgcgcg tggaagaacc atttatagct gcttgaatta tagcattttg
    23401 ttcagcatgc acacctctac aaagctcatg attttttcca gaaggaacat tttgctgttt
    23461 tcttaaacaa ccaatttctg cacagtgagg aacccctttg gggctaccat tataaccggt
    23521 tgaaataatt tgattatcgc gaaccaaaac tgcacctact tttctgcgga gacaagtgct
    23581 tcgctttgct gcaagatatg ccatttccat aaaatactgt tgccaggaag gtctttccat
    23641 tttttctcct gtagattttg tatttctcaa ttatcttata accctacttt ctgtcaatag
    23701 ctttgctgta acttaaaatc gactttttac ttaggggggt caggatttgt tactttcaat
    23761 aagaaaaaat gctcccaagt tatgaattgt aagaaagaaa aaagctggtt ttatgttacc
    23821 caaaagagaa agaacataca ttgtatccag gcagcagttg taaatgaaat tatatccagt
    23881 gtgtaaaggt aaaagatctg gaaacctgtt tcgggaggtt atcctaacta ctaaactgct
    23941 aaaaaatatg gatgatgcaa tgagcagata tctttaatac aagcttgata gtatattctt
    24001 tgtaggttga tgtggacatc gccctccgcc ctccgccctc agccctccgc cctccgctct
    24061 ccgccctccg ctaaaatgct cctaaactcc ttgtttctaa atttacatct cgccatttcc
    24121 ctatttcgct aatcgctatt atcagaattc ccttgccaaa aaatcccttt ccttatttta
    24181 atggattcat aataaaaaag gggaagttca taatggcaga taaattatat ttaattgatg
    24241 gcaccgcact attatacagg gctcattttg cctttatcaa aaatcctttg atcaacagta
    24301 aaggaaaaaa taccagtgct atctttggag taataaattc ttttttacat atcatcgaaa
    24361 atactcaggc agcttatgta gccatttctt ttgaccgtaa agctcctact tttcgtcata
    24421 ctctctatga agcatataaa gctcagcgtc ctccaatgcc agaagaactt actgcacaaa
    24481 ttgagccagt gaaaaaattc tttgaattaa ttggtctaaa ggaaataggg atggatggtt
    24541 acgaagctga tgatatttta ggaactatgg caaagcaatt caaagatcaa tttcaaattg
    24601 tgtttttaac cagtgacaaa gattactgtc aattaattga tgagcgttcc gtgatgcttg
    24661 atccgatgaa agatgtaatt ttggatcggg atgccgttta tgccaagtat ggagtttatc
    24721 ctgaacaatt tatagattat ttagctttgg taggagaccc ttccgataat attcctggag
    24781 taaaaagtat cggacccaag actgcctcaa tgttactttc cgagtataaa acgcttgata
    24841 atatttatgc ccatttggat tttataaagc cgcgctggaa agagctgctt ttagaaaaca
    24901 aagaaaatgc ttatctttca aaggagttag ctactattaa aactgatgtt cctattccca
    24961 aaccggattt agaatattta actttttgga cgaaggacct aaagaaggca attcctctat
    25021 tggaagaata tgaaataaat tccatcaagc gtaaaattga attaaactat ccagagccga
    25081 cagatacaat acaacaatct gaatatcaag atgatatctt taatatggaa agcaaaaaat
    25141 cagaagcggt ttcggaagct gacacaaaaa tgccttttaa agcaatttta gtgaataccc
    25201 gcaattttgt tactttgtta aaggaaataa aagaagcaca gctgatttcg cttgatacgg
    25261 aaaccgattc gccagatccg atgctggcaa atttagcagg aatatccttt tgtatgaatg
    25321 aagcaaaagc atactatctt cctttgggtc accagatggc ggataatcta tcccttccgg
    25381 aagttatccg tcatctcgaa gatgccatac aaggaaaact gctcttgggg cataacctca
    25441 agtttgattt aattgttctt tgcagacatg gattgaactt aaataatcct atttttgata
    25501 ctatgttggc tgcttatatt cttgatcccg gcactttaaa ttattcatta gatgcctgtg
    25561 cggtaagcga attgaattat aaaatgatcc ccatcagtga tttaattggt aaaggcaaaa
    25621 atcaatccac atttgattat gtagaattgg atagagcctg tatttattca gcagaagatg
    25681 cctgggtagt atataagcta tatccgattt accatagaaa agtagatttt agtgttatgg
    25741 cgaatctttt ttacacaatc gaattgcctc taataaaggt tttacaacga atggaaatga
    25801 acggtatggc cattgattgt tctgttttaa cggagatatc ccataaaata aatcaggaat
    25861 tgaagaaatt aacagatata atttatgctt atgcgggcta tgaatttaat ctcaattcta
    25921 cacagcaatt agcaaaaatg ctctttgaag aaaaaaagct cccaacccga aaaaaaacca
    25981 aaacaggtta ttctacggat aacagcgtgt tagaagaact ggcagaagat tatgaaattg
    26041 ctggtaccat aattcaatat cgacagcttg ctaaactgca atctacttat atcaacgctt
    26101 tacctaaaat gataaatcct gaaaccggaa gaatacattc ttcattcaat caaacagtta
    26161 cttccacagg acgtctttct tcttccaatc ccaatctgca aaatattcct gtccgtacag
    26221 aaattggacg cgaaatcaga aaggcatttt acgctaaaga tgaagagcat ttaattttgg
    26281 atgccgatta ttctcagata gaattgcgtt tgttggctct gatgagtgaa gatgaagttt
    26341 taatagaagc ttttaaacaa gacctcgata ttcataaaag aatggctgcc aagatttata
    26401 atgtatccct tgaacaagtt actccagatc aaagaagagc agccaagact attaactttg
    26461 gcattctcta cgg
//
LOCUS       DAOSTH010000017        24791 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_2191_length_24791_cov_13.560762, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000017 DAOSTH010000000
VERSION     DAOSTH010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 24791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24791
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2191_length_24791_cov_13.560762"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2929
                     /locus_tag="PLB65_03945"
     CDS             <1..2929
                     /locus_tag="PLB65_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77711.1"
                     /translation="MKLFRFRLIFLLLFLFIVFSTVIIAQKQTEEERYVVEDFKVEDI
                     PADDGTGLLLSWKPLERSKRIIEYRVYRGVSPDRLFFLEAIPVNVKTGVASDRMYYYD
                     NSGSEFFDITSPSELKKEKQQGKNSPLYQKMPRNIPILAEIAGKYSLLSVSEKNNYYY
                     RSQKVTLDKMPQSKTKSSFPEEGKTYAGLKDNQQTVFCFLNPGEKYYYSVVAVNERRL
                     YQKPAKITYGIPVPNPPNPAAAFHTVVLEDLKELRFEWELPIFSSDIVQYRIHKISAT
                     ADSLWNNIRYHPKVVDQHTVVLNQGLVGYGSMKNYCSVNIPQGSSLDEYKNAAYSLEL
                     IDADGYSSFSNLSKPRIKNSGNLPAKPIFHMEDKPNDKGDRLTVVWDHPICYVVKTTA
                     INEKYDKFRVNYQLNQTETQKVDNIFFEFYKNGEDKPFARINEFYQDNIVILKVPNGY
                     DYKKGFRVKIRMKGEPEIPEDYCLEQNLEYDPQMLAIMPTNALYRNGKNVSKINNIVY
                     RKSVTSPQLTLVKRNTSYDNSLDVNIPYASIIQKPVEGFSFAQGDSLITFINGERTAR
                     KLKSGDIKTPLALLSPKVDLVYDKDNATRIETNIFPDLGKKEAEEKVKELNTTLSELK
                     AQKAMLTDPAVVAQINQNIEQVQKQINAYSKNESLVSANKITDRKKRMRFIAKIREDY
                     TRWTSYQVVRSDGRGLFTESDMLKDGDDILYMKPISNVFDTNKYTTLVAMLIFGIMVV
                     VFISLAKRGKDLYIRPIAGLEEIDTAVGRATEMGRPIMYMMGHGSVSDVATIASMGIL
                     SLVARKTAEYDIKLIVPVYNYIVMPIAQEIVRDAHYSAGRPDSFDKNNVFFLTDVQFA
                     YVAGVNGIMIRERAATNFFMGYFSAEALLMTETGNSLGSYQIAGTDAVTQIPFFITTC
                     DYTLIGEELYAASSYLNREPMQLGTLKAQDYYKFLIFAFVVVGAVLSSFQLTGLAEIF
                     PVK"
     gene            2942..3571
                     /locus_tag="PLB65_03950"
     CDS             2942..3571
                     /locus_tag="PLB65_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77712.1"
                     /translation="MKKTIPLIIVILGGIIFVIYAFSPHKLIQDDFYNKFYIPWVYAS
                     IPWAALLGVMSLTMMHSNKIKRRAPKWQYSYFFFAGFIITSLAGFIGGTQKGSALMWI
                     FENMQMPMSSTMFSLLAFYMASAAYKAFRARSAEATVLLIAAIVVMLAQVPLGVKIYK
                     NLPVISQWILDVPNLASKRGIAIGVGLGGVATSLKILLGIERSYLGGTD"
     gene            3591..4772
                     /locus_tag="PLB65_03955"
     CDS             3591..4772
                     /locus_tag="PLB65_03955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77713.1"
                     /translation="MNFFERMQNLDRRWIYIVVALAIIIPLMIPYNSDNVTSPPTENL
                     YQMIDSFAGREDRAVLLSFLHDASTMSELYPMEIAIIRHCFERNIKVFALSFLTSGAP
                     IIDYAFNSVKEEYPDIKSGVDYCNFGYKPQPMAIVLGMGDNIANAVNTDAEGRKLESL
                     PIMKGINNYNEMNLVVEFSGSTAGTYWIYYARPKFGVNVALGVTAVMAADEYPYLQSG
                     QLIGMLSGLKGAAEYEKLVDVFAAYRDPKVDYRVKMDAEGNKIIPGRPFGKEVLNDEG
                     TKKLINITTQTKAEFTPADYAAFVAKYPEEKAIFDSLKELEGEKIIIDVSKITPELRN
                     QMGEYAYREINQLTHNINYKFKVARIGMNAQSVAHIMIIVFIIMGNIGYFIQKSKVVE
                     K"
     gene            4790..5392
                     /locus_tag="PLB65_03960"
     CDS             4790..5392
                     /locus_tag="PLB65_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77714.1"
                     /translation="MSFELFSNLVAAFFTLCIFSFLYKDNPLYQMAEQLLVGISLGYS
                     LVFTYERIFIPYIWQPIVLKHNFILIFPTLLGILYLFRFSRKLGWLSRYPIAFSMIGI
                     GLGVPLGIHAGILVQMRQAMVPVENINLFLILLGTIAVLLYFFFSKAHTGAYGKFVGI
                     GKWYMMIGFGASFGMTVMARISLLIGRIQFLVIDVLGLMK"
     gene            5486..8275
                     /locus_tag="PLB65_03965"
     CDS             5486..8275
                     /locus_tag="PLB65_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77715.1"
                     /translation="MKKYLILSCIILVIAISLLSTDEAKPESLKGKVTEFSATDMPFD
                     DGAGIVLKWKPLDKSHRVIKYNIYRGVSPDSLFLLNSIEVDPKLGVLANELFYYDRGE
                     QPLVEFETAPMKLKKEKQQNNDSPLYRKFPQQPELLASMLHRYNVLGATNASKLYYKS
                     SPISKDEDILAGLKLRCFDYIMAFPMAGQDYYYTILAINERGQYLPYADIQKVIPEDN
                     PPEAAAIFSSAYFADTGILNYEWIPPSSSPDIAMWEGWLIPKSLKPANGEVLSENWQA
                     SAMPLFQIPNYYGSESACHSVNTKEEGIKLPPNMEDYYTVLSYSDYLGQSAAVPAKNY
                     RILNSSQQLKMPSIEVKDTMNDKGDYLTVSLGKPIVFVSHALYLNKAKTALKINYEVS
                     ENELYKIKELQFTILDSLDRKIGKVSEYYLDKTVNFKLPAQYKHISNMKVRIAVLAKG
                     DKDYEDYSEQRIVYDTQNKVFRGENIYYENEPLNKLYYEILTHNSFDPDFMFGNRTNG
                     LTRAYGHPIPYESTVYHLITDYDTKSKQMLMNPQLTIAVDSDKGYSFAVPLFRDKFEK
                     DLQKQKEELDNLLKQKEQYANAPIPDSLQFAIQEAEGSYNFIINHPAYLKSQKASNNK
                     AWLKTLLATRNQNMRSYSYQVLKTDGKANFIVTSTYADAKGNTYFYPKSEWFDSTKYI
                     TLFASILLCILLIYAIIISRRGDVYIRPIAGLESIEEAVGRATEMGRPLMFVPGWGTL
                     GDPDTVASMMILGQVAKKAAEFDVRLISPHCDYMVMPMAQEIVQNAFNEVGRPDAYNP
                     NDIFFVSYDQFPFCAGVNGLTVRERVATIFYMGFFNAEALLLTETGNQTGAFQIAGTD
                     AITQVPFFITTCSYTLIGEEFYAASAYISRNPDLVSMLKASDYFKVIIVAVIIIGTLL
                     ASFNLTGFINAFPIE"
     gene            complement(8625..12890)
                     /locus_tag="PLB65_03970"
     CDS             complement(8625..12890)
                     /locus_tag="PLB65_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOQ77716.1"
                     /translation="MSKQTIFSTVHIEGSLLSNEFLQCLINGDQTLEGIKPEDYYLSP
                     TERLNEAATRSYLRLTGVWESFKNHIKDLADDDIGTTETRERWLYPLFHELGYNALQT
                     QKSIEIEGKFYPISHKSSEPVAIHLISYKWDLDRINPSARGSARRSPHSLMQEFLNRS
                     SEHLWGFLANGYKLRLLRDNVSLTRSSYIEFDLQAMMDGEVYSDFFLLWMLCHQSRLK
                     VDLDDFGKPLPPESCFLEKWYNFSQSEGIRIWDELRDKVKLAIEAFGCGFLSHPSNSI
                     LKENLSSGKLTKSEFYHQILHLVYRIIFILVAEERNLLLPPDLPDNLKKIYYQYYSLQ
                     RIKKIAKRKRGTKHSDLYKQMRITFTGLYSGNEILGLPALGSFLFKEESTSALNNCEI
                     ANSDLLTALRFLCYTQKNNVYQEISYRNLGSEELGSIYESFLEMHPDLNIEAGTFVLK
                     VISGSERKTTGSYYTPDSLVNCLLDTALEPVINSALKKANTEDNSPENQEKALLNLKI
                     CDPACGSGHFLLKAAHRLAKRLASLRAQEEEPSPLVIQNALRDVISHCIYGVDLNPLA
                     VELCKISLWMDALEPGKPLTFLDHHIKCGNSLLGCTPQLLKEGIPDSAFTPLTGDDKS
                     YCSYYKKRNKEERSGVQDIFTPDNKNWSYEQNIAPSQRNLNQMPEENLADLQKKEQAY
                     QELQASESYLNSKFIYDAWCAAFVIKKTEQESYPYLITQGILYKISENPYYIDPKLRK
                     EIIRLADVYQFFHWHLAFPEVFSADIANDDNAEQTNQLPIKKTPGFDCVLGNPPWEKV
                     KLQEKEFFAARDTDIANAPNASKRKELIAALPNTNPDLYQEFRKQLRIAEASSLLIRE
                     GGNFPLCGIGDLNTYTVFAELNRNLLNNIGYCGCIVPSGIATDDTTKMFFSSLIITGS
                     LHSLYDFENRKGIFPGIHRSFKFCLLTIAPLNLEYILNKKNSTEPAIADFVFFATSIA
                     DLKDPERHFKLSQDDIELLNPNTLTCPIFRNKSDAELTKYIYRRVPILINENYGEEGN
                     PWGISFLRMFDMSNDSYLFKTKIELEAEGFELSGNHFTKGEEHFLPLYEAKMIHHFNY
                     RFGDYTYLPEGSVSTQLPKVPLNRLQDPHYEPLPRYWVNIKEILQKLLNPGILFGFRD
                     ITNVTNERTFISSAFPLSAVGNTLPLISSSKYDLLPLLLCNMNSFCCDYLIRQKVGGT
                     HMTYNYVKQFPVLTDNQYSAQLFQFIISRFIELSFTSYGMTNNDVTIYNGPPFIWDEE
                     RRFEILCELDALYFHLYLGTQKEWEKSYNSSSQESSESASGNTINPLLAYFKTPRKAV
                     EYIMETFPIVKRKDEEEFGEYRTKLRILAIYDQMTPLFEDSSDRIETSTYISALNPPP
                     GPPCDENGNFIPPEKWDINSWPSHIHQQIIMDKVIAPESCQPR"
     gene            complement(13158..16022)
                     /locus_tag="PLB65_03975"
     CDS             complement(13158..16022)
                     /locus_tag="PLB65_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOQ77717.1"
                     /translation="MEQLQPDKSSNDIPEDYAIGSLVSVRGREWVVQPESTSELLIVK
                     PLGGSDDEITGILTKLEEVHPASFSLPNPNELGDYQSCNLLRNALRLGFRNSAGPFRS
                     FGRLAVNPRPYQLLPLLMALKLDPVRILIADDVGVGKTIEACLIARELLDRGEISRIC
                     VLTPPHIAEQWQSELLDKFHIDAELVLSSTVRQLEKNCAQNESVFEVYPFTVVSIDYI
                     KSGRHKDEFIRTAPEMIIIDEAHTASFDSVSTSSRHQRYELVNALSKDPLRHLILVTA
                     TPHSGNVNAFRSLLSLLNSEFTAYPDDLTGTENENYRKTIAKHFIQRRRADIEHYLED
                     TSFPEAIIEELSYKLSEPYRQLFSKALNYAREIVQDKEGGDLHRRIRWWSALSLLRAL
                     ASSPAAALKTMNNRNATQETDSPFEADELGRRQVYDTYDRDATESSDIVPGSISSEEE
                     NSYSNKKLRALAQEAESLFGELDYKLLNIINPLKTYLKEGFKPIIFCRFIQTAEYLTD
                     QLKQRLPQDVEISAVTGVLPPEEREKRIIELSKHKKRVLVCTDCLSEGINLQNYFDAV
                     IHYDLSWNPTKHEQREGRVDRFGQTSKKVKLLTYYGTDNQIDGIILDVLLKKHHQIKS
                     TLGVSVPVPYDSEQVLEAIFEGLLLREQESSSEQLLLPFLDDFLAQDKQKLHLAWDKA
                     KDREKLSRSIFSQQALSKDLKEITTKELDKINESIGSRLDIRNFVKHSLIRFNASIIE
                     HEDTLQIDASAVPTFVKESCYFPSDKLTLKFELPVSENEIYLPRTHPIVEGLADIAIN
                     TALEGNSAIAKRCGVIRTSSVERKTTLLLMRYRFHLIEAFHHKSYPILVEDSQILAFQ
                     GKPEEPEWLENKEISKLLESIPAVNVSPEFAKEQLENVILNYETLLPHLNTFAEKRAR
                     ELLSAHTAVRDLSLIRKSVKPYVKALLPADLVGVYIYLPV"
     gene            complement(16100..21226)
                     /locus_tag="PLB65_03980"
     CDS             complement(16100..21226)
                     /locus_tag="PLB65_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ77718.1"
                     /translation="MNIFELRDKIIEEYSSFVNGFINIKDSKASNYVLDKINKGLLWP
                     DPLVQINPFFTLGESVKELVSQGFLHPDCEKIFCLRNEKEIGSKPLQLYKHQSDAIKK
                     AVANESYILTTGTGSGKSMAYIIPIVNHILQTGSGKGIKAIIIYPMNALANSQKQELE
                     KFINPHHKSSANNISFRCYTGQENDAERQEILANPPDIILTNYVMLELILTRPDETPL
                     VSASKNLKFLVLDELHTYRGRQGADVAMLIRRVKEATGTKDVIFVGTSATLSSGDIET
                     QKQEIAKVSSLLFGIDFNQGNVIGESLERITAEYDFTNRDELELLKKEISEVINVPKL
                     EFEQIKHSHLASWIESNFGIRTDPKTGILVRSEPISITGPKGGARLLHNLTDKENELC
                     VRAIQKMLMLGFQTLNPINSFPVFAFRLHQFISRGQTVYASLEDNDSRYFTMQKQLYV
                     PDTNKLKVLLPLTFCRFCGQDFYTVHRTLNKDNETLYTPRELYDIYLYEEDAGFLYLP
                     DNPDCDLTAMIPPEWKDINGNIIAARKYDMPEKVSVRPDGIENPAGRIMYFFKTPFRF
                     CPSCGVSFDATQRSDFPKLTALGTEGRASATTILSLSVIRNIKQMDMEDKAKKLLSFT
                     DNRQDASLQAGHFNDFIQIGLLRGALYQALVNAGSDGIRHSELAQKVFDCLNLNYEEY
                     ASNPLADRDSARIYTERALCDVLGYRLYYDLRRGWRITAPNLEQTGLLKIDYLALEEL
                     AYSEEEWQNCHPALSSCDHQARYNILKTLLELMRRSLTIKVNYLDPRFQDIMLQNSHQ
                     RLIPPWGFDESTQSRNLTYASILYLDPNSNARNRGSNLYVTPRSSYGQYLRRATTFKD
                     YKTTIDLQQTKTIIENLIDRLTRYGIIEKVDDKNNGYQLVADSIIWKQGDGKTGYYDP
                     LHQPTESLEGQPVNEFFKEYYSTIALTTHKIYAREHTAQVSYEERKKRETAFREGTLP
                     ILYCSPTMELGIDISWLNVVNMRNIPPTPANYAQRSGRAGRSGQPALILSYCSTGSPH
                     DQYFYKHPEKMVSGVVSTPQIDIANENLLHSHINAIWLSEAKLSLGRALNNIIDISTE
                     KLELLPSVKSTLNNSELQNRAFERAESVIATVQNYLQSTIWFNNDWLTDVIKQIPNEF
                     EQACERWRDLYRAALNQQQLQNSIILDVSRSAADRKQAKQLRKEAETQLNLLTSIEGA
                     FQSDFYSYRYFASEGFLPGYNFPRLPLSAYIPARGTVKGEDEYLNRPRFLAITEFGPR
                     SLIYHEGSKYIISQVIMPPTRDNNNLPSLASMKLCAECGYLNGYENDICENCKAQLDS
                     PLTNLFRMQNVVAKRREQISCDEEERLRMGYEMRTTIRFAKKGGKLSCFQTELYSENE
                     LLAKLTYGDSADIWRINMGFKKNHRGRQLGFWLDSERGFWERNPDTDNEDDSPLGNRR
                     ILVCPYVSDTKNCLLFEPQFNAEPAVMASLQSALKNAIQQVFDIEDNELAAEPLPNRN
                     VRHSILFYESAEGGAGVLKRLMDEQNFRKTIRKALEICHYDPDSFTDLEMLKNASERC
                     EAACYDCLLSYKNQLDHLLLDRKSIIIHLKEMLNADFKRSSSEVTRLELYHQLRNLCG
                     SELESKWLDFIYNNNYILPLHAQQLIEKAQTRPDFTYPDIYVAIYIDGQVHDFSNNIE
                     KDREVDARLDDLGWKVIRFLYNQDWQTIIDQHPEIFGTRK"
     gene            complement(21471..22811)
                     /locus_tag="PLB65_03985"
     CDS             complement(21471..22811)
                     /locus_tag="PLB65_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77719.1"
                     /translation="MKYINLLLAFLVAGILFAQPNFMPVTTIAEDFGSLSCPNCLVAW
                     QGLQAVHSETHNGEFITARLFTESGSLSNAEIEERFSYYGVIGLPNVIFNGLISIEGA
                     GEGTADGTNYLNALSQTRFSASPVKIDITDFSLGRGAVSANVTMLSPTAVISNAKLVY
                     YLLEDNVGSETNVLRGVLYDDIASLSGAGTTLNFNRTFDLNPDWSVSNLWGLIFIQLS
                     DKTIVQCVSSLPLPEYNFRVAMDWKTSVTVEPNTNYLSSPIWLFNLGAADNYTMSIQV
                     DNAPADWYFNYCDEEGNCYPGSIDRPFTLAAGEATSIHLNLMIGSSGTADFRIAVNSP
                     HIGSYNVPFHITASTPIIDDTNTLSPYIIKGFYPNPVKSSAVFHLSNPKNVGSATIEI
                     YNVKGQKVQTLKETNLHSGENEISFVPDTNLPNGIYYYRLQNSNSKAGRFIIMK"
     gene            complement(23025..24467)
                     /locus_tag="PLB65_03990"
     CDS             complement(23025..24467)
                     /locus_tag="PLB65_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HOQ77720.1"
                     /translation="MKHFLLILILVSFTLLGAQNTLWVNGSNEAQFIYRTVDDSLHTY
                     FKDAFGFNLGYRNFSFGMKFIAELPKYSTEQTELMDELDANRLELGWQELYASYEKDA
                     CKIYAGITEESFGNGIVFRSYKDLEFDIDNRLESFLFSYNDDFKFKAIYGAIENPNII
                     GRYDLAYGADLQTPYFKGLSFGASAMAFRNLLATNIYNQRTVFAGRLNYSAHNLDLQA
                     ETAFSELYHQPGIGTKNGKAIYVNGSYLIGFITIGGAYKQYDKFQYRLNDLPMANYHN
                     ETLSDASASGEDEIGYQGFGTVNFTDNLNFTVDYAEAFNSDKDKKMNDAYFALEYLGN
                     SYSLLASYSQIEKIDDINSAWQQELIPALQSSFYLFNIPVKIQAEYKKISKQKQITES
                     EHYEPKLQTDFALGKLALSVCVQSSWEEISEMLDSRYYPSAEIKYPLFEHSDIILFGG
                     KEAGGKVCRNGVCRYVAPFEGLRIELNTRF"
BASE COUNT         7590 a         4528 c         4843 g         7830 t
ORIGIN      
        1 tatgaagtta ttcagatttc gcctaatctt tctccttctg tttctattca tagtgtttag
       61 cacagtgata atagcccaga aacagacgga ggaggaaaga tatgttgtag aagatttcaa
      121 agtagaagat attcctgctg acgatggaac gggattatta ttatcctgga agcctcttga
      181 acgaagcaaa cgcattatcg aatatcgtgt ttaccgggga gttagtcctg acagattatt
      241 tttcctggaa gccatacctg ttaatgtaaa aacgggtgtt gcatcggata ggatgtatta
      301 ttatgacaat tctggtagtg aatttttcga tattacctct cccagcgagt taaagaagga
      361 aaaacagcag ggtaagaata gtcctctata tcaaaaaatg cctcgtaaca ttccaatttt
      421 agcagaaata gcaggtaaat attctctgct ttccgttagt gaaaagaaca attactatta
      481 cagaagccag aaagtaactc tggataaaat gccacaatca aaaacgaaga gttcttttcc
      541 tgaagaggga aagacatatg ctggtttgaa ggataatcag caaacggttt tttgttttct
      601 aaatcctggc gaaaagtatt attattcagt tgtagcagta aatgagaggc gtttgtatca
      661 aaaacctgcc aagatcactt atggaattcc ggttcccaat cctcctaatc cagctgctgc
      721 ttttcacact gtagttttgg aagacctaaa agagttaagg tttgaatggg agcttcctat
      781 attcagttcg gacatagttc aatatcgaat tcataaaata tcagctactg cagattcttt
      841 atggaataat atacgctatc atcctaaggt tgtagatcag catacggttg tgctcaatca
      901 gggtttagta ggttatggtt caatgaagaa ttactgcagt gttaacattc cccaaggtag
      961 ttccttagat gaatataaaa atgctgctta ttcactggaa ttaatagatg cagatggcta
     1021 cagcagtttt tccaatcttt ccaagccgcg gatcaagaat agtggtaatc ttcctgccaa
     1081 gcctattttt catatggaag ataaacctaa cgataaaggt gatcggttaa cagttgtttg
     1141 ggatcatcct atttgttatg tagttaaaac cacggcaata aacgaaaaat atgataaatt
     1201 cagagtgaat taccaattga atcagaccga aacccaaaaa gtggataata tcttttttga
     1261 gttttataag aatggcgaag ataagccctt tgctcgaatc aatgaatttt atcaagacaa
     1321 tattgtgatt ttaaaggtcc ccaatggtta tgattacaaa aaggggttcc gagtgaaaat
     1381 tagaatgaaa ggcgagcccg aaattccaga ggattactgt ctggagcaaa atttggaata
     1441 tgatcctcaa atgcttgcca ttatgcctac taatgcacta taccgcaatg ggaaaaatgt
     1501 ttccaaaatt aacaatatcg tttatcgtaa aagtgttact tctccccagt taactttggt
     1561 taaacgcaat acatcatatg ataactcttt ggatgtaaac attccttatg ccagtataat
     1621 tcaaaagccg gttgagggat tcagttttgc ccaaggtgat tcgctgatta cttttattaa
     1681 tggtgaacga actgctcgca aactgaaaag tggagatata aaaactcccc tcgctttact
     1741 ttcgcccaag gttgatctag tttatgataa ggataatgca acccggatcg aaacaaatat
     1801 atttcccgat ttaggtaaaa aggaagcgga agaaaaagta aaggaactga atacaacgct
     1861 gtcggagttg aaagctcaaa aagcaatgtt aactgatcct gcagtagttg cccaaataaa
     1921 tcaaaacata gagcaggttc agaaacaaat aaatgcttac agtaaaaacg agagcttggt
     1981 ttccgctaat aaaattaccg accgaaaaaa gcggatgcgc tttattgcta aaattagaga
     2041 agattatacg cgctggacaa gctatcaggt agttcgttct gatggtagag gtctttttac
     2101 tgaaagtgat atgttaaaag acggcgatga tattctatat atgaaaccga tttccaatgt
     2161 gtttgacaca aataaatata ctaccctcgt agcaatgttg atatttggga taatggtggt
     2221 ggtctttatc agtttagcca aacgcggcaa ggacctatac attcgtccca tagcaggttt
     2281 agaagaaatt gatacagctg ttggcagagc cacagaaatg ggtcgcccga taatgtatat
     2341 gatgggtcat ggtagtgtta gtgatgtagc aacaattgct tcaatgggta ttttaagttt
     2401 agttgctcgc aaaacagctg aatatgatat aaaacttatc gtgcctgttt acaattatat
     2461 agtgatgcct attgcccaag aaattgttag agatgcgcat tattctgcag gccgtccaga
     2521 tagctttgat aaaaataatg tattcttttt aaccgatgtt cagtttgctt atgtagcagg
     2581 tgtgaatgga attatgattc gtgagcgagc tgccacgaat ttctttatgg gttattttag
     2641 tgcggaagca ttgttgatga cggaaacagg aaattccctg ggttcttatc agattgcagg
     2701 aaccgatgct gtaacccaga ttcctttctt tataactact tgtgattata ctttaatcgg
     2761 agaagagctt tatgctgctt cttcttattt aaatcgtgaa ccaatgcaac tgggaacttt
     2821 gaaggcacag gattattata agtttctcat tttcgctttt gtggttgtcg gagcggtgct
     2881 ttccagtttt cagttaacag gtcttgcaga aatatttccc gttaaataaa tgaggtctta
     2941 gatgaagaaa acaatacctt taatcattgt aatcttgggt ggcataatct ttgtgatata
     3001 tgcctttagt ccccacaagc ttatacagga tgatttttac aacaagtttt acattccctg
     3061 ggtttatgct tccatccctt gggctgcatt actgggagta atgagtttaa ctatgatgca
     3121 cagcaataag ataaaaaggc gtgctcctaa atggcaatat agctatttct tttttgctgg
     3181 attcattatt acttctcttg ctggttttat tggtggaacg caaaaaggaa gtgctttgat
     3241 gtggattttt gagaatatgc aaatgccgat gagctctacg atgttttcgt tattggcatt
     3301 ttatatggca tctgcagctt ataaagcatt tagggctcgt tctgcggaag cgacggtttt
     3361 actgattgct gctattgtag tgatgttggc acaagtgcct ttgggagtga aaatctataa
     3421 aaatctaccg gttatttctc aatggatttt ggatgttccc aatcttgctt ccaagcgtgg
     3481 aattgctatt ggagtaggtc ttgggggtgt ggcaacttcc ctaaaaatac ttttgggcat
     3541 agaacgttcc tacttgggtg gaacggatta aggaaagggg ggaaaacagg atgaattttt
     3601 ttgaaagaat gcaaaattta gaccggcgct ggatctacat tgttgtagct ctggcaatca
     3661 tcattccctt aatgattcct tataattcgg ataatgtaac ttctccgcca acggagaatc
     3721 tgtatcagat gatagattct tttgccggca gagaagatag agccgtgtta ttaagttttt
     3781 tacatgatgc ttccacgatg tctgagcttt atccaatgga aattgccata atcagacatt
     3841 gttttgaacg caatatcaag gtcttcgctc tctcttttct aacttcggga gcacctatta
     3901 ttgactatgc tttcaattct gtgaaagaag aatatccaga tattaaaagt ggagtggatt
     3961 attgtaattt tggctataag cctcagccta tggccattgt tttgggtatg ggagataata
     4021 ttgccaatgc tgtaaatacg gatgctgaag gacgcaagct ggagagtttg ccgattatga
     4081 aaggaattaa taactacaac gaaatgaacc tggtagtaga attttccgga agcacggcag
     4141 gaacttattg gatatattat gctcgtccca aatttggagt gaatgtagct ttgggagtta
     4201 ccgctgttat ggctgctgat gaatatcctt atttacaatc gggtcaattg attgggatgc
     4261 tttctggact aaaaggtgca gctgaatatg agaagctggt tgatgttttt gccgcttatc
     4321 gtgatccgaa agttgattat agagtaaaga tggatgcgga aggcaacaaa attattccgg
     4381 ggcgtccttt cggcaaagaa gtgctcaatg atgaaggcac gaaaaaacta attaatatta
     4441 ccactcagac caaggcggaa tttactcctg ccgattatgc tgctttcgta gccaaatatc
     4501 cagaggaaaa agctattttc gattctctca aagagctcga aggggagaaa ataattattg
     4561 atgtaagtaa aataaccccc gagctaagaa atcagatggg cgaatatgcc tatcgcgaaa
     4621 taaatcagct tactcacaat ataaactata aatttaaagt tgccagaatt ggaatgaatg
     4681 ctcaatctgt tgctcacata atgattatag tttttatcat tatgggtaat attggttatt
     4741 tcattcagaa atccaaagtg gtagaaaagt aaagaacggg aggataacaa tgagtttcga
     4801 attatttagt aatctagtgg ctgcgttttt cacgctatgt attttctcct tcctgtataa
     4861 ggataaccca ctctatcaga tggcggaaca acttttggta ggaatttcct taggttattc
     4921 tttggtcttt acttatgaac ggatatttat tccttatata tggcaaccga ttgtgctcaa
     4981 gcataatttt atccttattt ttcctaccct tttaggaata ctgtatctgt tccgtttttc
     5041 tcgtaaactg ggttggcttt ctcgttaccc tattgctttt tcgatgattg gaattggatt
     5101 gggtgttccc ttaggaattc atgctggaat tttagttcag atgcgtcagg caatggttcc
     5161 tgtagagaac atcaatctct ttcttatttt attaggcaca attgcagtgt tgttatactt
     5221 cttcttttcc aaagcgcata ctggtgctta tggaaagttt gtaggcattg ggaaatggta
     5281 tatgatgatt ggttttggag cttcatttgg aatgacagtt atggctcgta tctcattgct
     5341 gattggtaga atccagttcc tcgtaattga tgtgctggga ctaatgaaat agctgttatt
     5401 tccaggaacc ggtaaaaaag gtaagacaaa aaaacaaagg ttcttaccca attacttact
     5461 ggaatcctgg ctaagggata gataaatgaa aaaatacctg attttaagct gtataatctt
     5521 agtgatagca atttcattgc tttctacaga tgaagccaaa cccgaatccc tgaagggcaa
     5581 agtaactgaa tttagtgcca ccgatatgcc ttttgacgat ggtgctggaa tcgttttaaa
     5641 atggaaacct ttggataaat cacaccgtgt gattaaatat aatatataca gaggcgttag
     5701 ccctgattct ctgtttttac ttaacagcat cgaagttgat cctaaactgg gtgttttagc
     5761 caatgaacta ttctattatg accgtggtga acaaccatta gtagaatttg aaacagctcc
     5821 tatgaagctg aaaaaagaaa aacagcagaa taatgatagc cctttgtacc ggaaatttcc
     5881 tcagcaacca gaattgctgg cttcaatgct gcatcgctat aatgttttag gagccacaaa
     5941 tgcttctaaa ctgtactata aatcttctcc aataagtaaa gacgaagata ttctggcagg
     6001 tttgaaattg cgttgtttcg attacattat ggcgtttcct atggcgggac aggattatta
     6061 ttatactatt ttggcaatta acgaacgcgg gcaatatttg ccttatgcgg atatacagaa
     6121 agttattcca gaagataatc ctcccgaggc agctgcaatt ttttccagcg cctattttgc
     6181 tgatacagga atattgaatt atgaatggat tcctccttct tccagtcccg atattgctat
     6241 gtgggagggc tggctaattc ccaaatctct aaaacctgca aatggagaag ttctttctga
     6301 aaattggcaa gccagtgcta tgccgctatt tcaaattcct aattattacg ggtcggaaag
     6361 tgcttgtcat agcgtaaata caaaggaaga aggtattaag ttgccaccca atatggaaga
     6421 ttattatacc gttctttcct atagcgacta tttaggtcaa agtgcagcag ttccagcaaa
     6481 aaactatcgc attttgaatt cttcgcagca gctgaaaatg ccgtctatcg aagttaaaga
     6541 cacaatgaac gataaaggtg attatttaac tgtttcgctt ggtaagccaa ttgtttttgt
     6601 aagccatgca ctctatctga ataaagccaa aacagcattg aagataaatt atgaagtttc
     6661 ggagaatgaa ctatataaaa ttaaagaatt gcagtttact atacttgatt ctctggatag
     6721 aaaaattggc aaggtctcgg agtattatct ggataaaaca gttaatttca agcttcctgc
     6781 ccaatataaa cacatcagta atatgaaggt ccgtattgca gttcttgcca agggcgataa
     6841 ggattatgag gattacagcg aacagcgcat tgtttatgat acccaaaata aggttttccg
     6901 cggagagaac atatattatg agaatgagcc actaaataaa ctctattacg aaatccttac
     6961 acataatagt tttgatcccg attttatgtt tgggaatcgg actaatggtc tcaccagagc
     7021 ttatggccat cccatacctt atgaaagtac tgtatatcat cttattaccg attatgatac
     7081 aaaaagtaaa cagatgctaa tgaatccaca gcttactatt gctgtagatt cagataaggg
     7141 ctatagtttt gcagttccac tattcagaga taaatttgaa aaagatctgc aaaagcaaaa
     7201 agaggaactg gataatttgc ttaagcaaaa agaacaatat gcaaatgctc caatccccga
     7261 ttcgttacag tttgcaattc aagaagcaga aggcagttat aattttatta tcaaccatcc
     7321 tgcatatctt aaatcccaaa aagcaagcaa taataaagcc tggctaaaga ccttactggc
     7381 aacccggaat caaaatatgc gcagctattc ctatcaggtt ttaaaaaccg atggcaaagc
     7441 aaatttcatt gttacttcca cttacgctga tgcaaagggt aatacatact tctatcctaa
     7501 aagtgaatgg ttcgattcca ccaaatatat cactctcttt gcctccatct tgttgtgtat
     7561 attacttatt tacgccatta ttataagtcg cagaggagat gtttatatca gacccattgc
     7621 tggattggaa tccattgaag aagcagttgg cagagcaaca gaaatgggtc gtcctttaat
     7681 gtttgttccc ggatggggta ctttaggaga tccggatact gttgccagta tgatgatttt
     7741 gggacaggta gctaaaaaag ccgccgaatt tgatgttcgc ttaatttctc cgcattgcga
     7801 ttatatggtt atgcctatgg ctcaggaaat agttcaaaat gcttttaatg aagtaggacg
     7861 tcccgatgcc tataatccca atgatatatt cttcgtttcc tacgatcagt ttcctttctg
     7921 cgcaggtgtg aatggattaa ctgttaggga aagagtggca accatattct atatgggatt
     7981 tttcaatgcg gaagcactac ttttaacgga aacaggaaat caaacaggtg ccttccaaat
     8041 tgcgggaacc gatgccatta ctcaagtgcc tttcttcatt actacctgtt cttatacttt
     8101 gattggtgaa gaattttacg ctgcttctgc ctatatttcg cgtaacccgg atttggtaag
     8161 tatgctaaaa gcctccgatt atttcaaagt aatcattgtt gctgttatta taataggaac
     8221 gctacttgcc agcttcaatt taactggctt tatcaatgcc ttcccgatag aataggttaa
     8281 gataagcaaa ataacagagc gggatttatt ctcgcaatat atacagatta gatagctgaa
     8341 aagggttgag agcgttgatc cctataaaat cctgcgctta atgctctcaa ccctcttgcc
     8401 tatctaaatc ctgataatct tgataattct gcttcctcaa ttccaaaata taactctacc
     8461 accaaaccga agcatctgct ataaaactta attggtgctt attatatatg gtgaagatac
     8521 ggtgctgcta ctcggcaaaa acccgaatat caccattgca tttccaccac agatagcaag
     8581 gaaggacttt ttgctatgca gtaaaaaatc aagcggatta cagattaacg aggttgacaa
     8641 ctttctggtg caataacttt atccataatg atttgctgat gaatatgaga gggccaggaa
     8701 ttgatgtccc atttttccgg agggatgaag ttaccgtttt catcacaggg tggaccaggg
     8761 ggtggattta aagctgaaat ataggttgag gtttctatcc tatctgaact atcctcgaaa
     8821 agaggggtca tttgatcata aattgctaat atacgaagct tggtacgata ttctccaaat
     8881 tcttcttcat ccttgcgttt tacaatcgga aaggtctcca ttatatattc cactgctttc
     8941 ctgggagttt taaaataggc aagaagagga tttatggtat tgcccgatgc tgattctgaa
     9001 gattcttgag aagaggaatt ataactcttt tcccattcct tttgcgttcc caaatagaga
     9061 tgaaaataaa gcgcatccag ttcacaaaga atttcaaaac gacgctcctc atcccaaata
     9121 aaggggggtc cattatatat ggtaacatca ttattggtca taccatacga agtaaaggaa
     9181 agttctataa atcttgaaat gataaactgg aagagttggg cagaatattg attatctgtt
     9241 aatactggaa attgttttac ataattatag gtcatatgtg tcccacctac tttttgccta
     9301 attagataat cacaacaaaa actattcata ttgcacaaaa gtaatggcaa tagatcatat
     9361 tttgagctac taataagtgg aagagtattt ccaacagcac ttaaagggaa agcagatgat
     9421 ataaaagtcc tttcgtttgt tacatttgtg atatctctaa agccaaataa aatccctgga
     9481 ttaagtagct tttgcagaat ttctttaata tttacccaat agcggggtaa gggttcgtaa
     9541 tgtgggtctt gtaatctatt taatgggact ttgggtaatt gagtggaaac cgaaccttca
     9601 ggtaaataag tataatcccc aaaacgataa ttgaaatgat gaatcatttt tgcttcatat
     9661 aggggtaaaa agtgttcttc tcccttggta aagtgattac cggaaagttc aaagccctct
     9721 gcttccaatt ctatttttgt tttaaagaga taagaatcgt ttgacatatc gaacattctt
     9781 aaaaatgata ttccccaagg gtttccttct tctccataat tttcattaat aaggatagga
     9841 acccggcggt agatatattt agtgagttcg gcatcgcttt tatttctgaa aatagggcag
     9901 gtaagggtgt tgggattaag caattcaatg tcatcctggc tcagcttaaa atggcgttcc
     9961 gggtctttta aatcggctat ggaagtagca aagaagacaa aatcggcaat tgcaggttct
    10021 gttgaattct tcttatttaa gatatattca aggttaagag gagcgatagt taaaaggcag
    10081 aatttaaagc tgcgatgaat tcctggaaat atgcctttcc tgttttcaaa atcatagagg
    10141 gaatgcagag agccagttat aattaaagaa ctaaagaaca ttttagttgt atcatcagta
    10201 gcaataccac tgggaacaat acaaccacaa taacctatgt tgtttaaaag gttacgattt
    10261 aattctgcaa agacagtata ggtattcaga tcacctatac cgcaaagagg aaagtttccc
    10321 ccttctctaa ttagcagaga agaagcttcg gcaatacgaa gttgttttct aaattcctga
    10381 taaaggtcag gattagtatt aggtaaagca gcaattaact ctttgcgttt tgaggcattg
    10441 ggggcattgg caatatcagt atcccgagct gcgaaaaatt ctttttcttg cagtttaact
    10501 ttttcccaag gtggatttcc gaggacacaa tcaaagccgg gtgttttttt tataggcagt
    10561 tgattagttt gttcggcatt atcatcgtta gctatatctg cagaaaagac ctctgggaag
    10621 gcaagatgcc agtggaagaa ttgataaaca tcagccaggc ggataatttc ctttcgcagt
    10681 ttaggatcta tgtaataagg attttcgctt attttataca aaatgccttg ggtgattaga
    10741 tagggatagc tttcctgttc ggttttttta ataacgaaag cagcacacca ggcatcgtaa
    10801 ataaatttac tgtttagata ggattcggag gcctgaagtt cctgatatgc ctgttctttc
    10861 ttttgcaggt ctgccaggtt ttcttccggc atttggttta gatttctttg ggaaggtgca
    10921 atattttgtt cataactcca atttttatta tcgggggtaa aaatatcctg aactccgctg
    10981 cgttcttctt tatttctttt tttgtaataa gagcaatatg atttatcgtc tccggttaga
    11041 ggtgtaaaag cggaatccgg aattccttct ttcagcaatt ggggagtgca gcccaaaaga
    11101 gaatttccgc atttgatgtg atggtcaaga aaagtgagag gttttccggg ttccaaggca
    11161 tccatccaaa gagatatttt acaaagttca actgccaaag gattgagatc gacaccataa
    11221 atgcaatgac tgataacatc ccttagggca ttttgaataa ctaaggggga tggttcttct
    11281 tcttgagcac gaagggaagc cagtcgtttg gctaaacgat gagcagcttt caataaaaaa
    11341 tgtccgctgc cgcaggcagg atcacaaatc ttaagattca gcaatgcttt ctcttggttt
    11401 tcgggagaat tatcttctgt gtttgccttt ttcagagcag aattgataac tggttccaat
    11461 gctgtatcca gtaaacaatt aactaaggaa tcgggagtat aataacttcc ggttgtcttt
    11521 ctttctgaac cggagataac tttcaagaca aaggttccag cttcaatatt aaggtcagga
    11581 tgcatttcca gaaaggattc atagatactg ccaagttctt cggaacccag attgcgataa
    11641 ctgatttcct gataaacatt gttcttttga gtataacaaa gaaaacgcag tgcggttaat
    11701 aagtcgctat tggctatttc acaattgttt aaagcggaag tagattcttc tttaaataga
    11761 aatgaaccca aagctggtaa gcccaaaatt tcgtttccac tataaagacc tgtaaaagta
    11821 atccgcattt gtttataaag gtcactatgc ttggttcccc tttttctttt ggcgattttt
    11881 tttattcttt gcagagagta atactgataa tatatctttt taaggttatc gggcaaatct
    11941 ggagggagca gaagatttct ttcttctgca accaaaataa aaattatgcg atagaccagg
    12001 tggaggattt ggtgataaaa ttcggatttg gtaagcttcc cagagcttaa attttctttt
    12061 aatatagaat tggaaggatg agaaaggaat ccgcaaccaa atgcctcaat tgccagctta
    12121 actttatccc gcaattcatc ccatattcta ataccttcag attgggaaaa attataccat
    12181 ttttccagaa aacaactttc aggaggcaag ggttttccaa aatcatcaag atcaactttt
    12241 aaccgcgatt gatgacacag catccaaagc aggaaaaaat cgctatagac ctccccatcc
    12301 atcattgcct gcagatcaaa ttctatataa gaactgcggg ttaaagaaac attatctctt
    12361 aagaggcgta atttataacc attggcaaga aacccccaaa gatgttcaga actcctattc
    12421 aaaaattcct gcatcaaaga atggggcgac cttctggctg atcctctggc agaaggatta
    12481 atgcggtcta aatcccattt gtaactaatt agatgaatag caacgggctc tgaacttttg
    12541 tgactgatag gatagaactt tccttcaatc tcaattgatt tttgagtttg taacgcatta
    12601 tatcccaatt cgtgaaaaag aggatataac cagcgttctc ttgtttcagt agtcccaata
    12661 tcatcatctg caaggtcttt aatatgattt ttaaaacttt cccacacacc ggttaatctt
    12721 aggtaggaac gagttgctgc ttcattcaaa cgctccgtag ggcttaaata atagtcctct
    12781 ggttttattc cttccagggt ttggtcaccg tttattaagc attgtaaaaa ctcattagat
    12841 aaaagagagc cttctatatg cacagtggaa aagattgttt gtttagacat tagcatccta
    12901 ccttataatt taaactcaca tagctaagag agtttggcgt attaggggtt agaacccaga
    12961 taaaaattag ctttgcagct tctattaagc tataagctac agagtgaaga acaaaaacaa
    13021 caagattaca tatagtacta agaggaaaga tagtttcttc cctttccaga aggttttctg
    13081 cattttggct tagataatta ggcaattcca ttctttacta ctctctatta taataatctg
    13141 aattaacttt agaggtttta aactggtaga tagatatata cacctaccag gtctgctgga
    13201 agtaaggctt ttacataggg tttaacagat tttcttatta aagataaatc ccgcactgca
    13261 gtatgagccg ataaaagttc ccgggctctt ttttcagcaa aagtattcaa atgaggcaga
    13321 agtgtttcgt aatttagaat aacattttcc agctgttctt tggcaaattc aggagagaca
    13381 ttaacagccg ggatggattc aagtagtttg cttatttctt tgttttccag ccattcaggt
    13441 tcttcaggtt taccttgaaa agcaagaatc tgagagtcct ctactaagat aggataggat
    13501 ttgtgatgaa atgcctcaat taaatggaaa cgataacgca ttaacagaag cgtggttttt
    13561 cgctcaaccg aagaggttct aattactcca catctttttg caattgcgct gttgccttcc
    13621 aaggcagtat ttatagctat atctgccaat ccttctacta taggatgagt acggggaaga
    13681 tatatttcat tttcactaac gggaagctcg aattttaggg ttagtttatc ggaaggaaaa
    13741 taacagcttt ctttaacaaa ggtcggtaca gcggaagcat caatctgtaa tgtatcttcg
    13801 tgttctatta tagaagcatt aaagcggatt aagctatgtt ttacaaaatt tctgatatcc
    13861 aatctggaac cgattgattc gttaatttta tccagctctt tggttgttat ttcttttaag
    13921 tctttggata atgcctgctg agaaaatata gaccgactaa gtttttcccg gtctttggct
    13981 ttatcccaag ccagatgtaa tttctgttta tcttgggcta ggaaatcgtc aagaaaggga
    14041 agcaaaagct gttctgaact gctttcctgt tcccttaaaa gcagaccttc aaaaattgct
    14101 tccagaacct gttcggaatc gtaaggaacg ggaactgaaa cacccaaggt agattttatt
    14161 tgatgatgtt tttttagcag aacatccaga ataatgccat ctatttgatt atccgttcca
    14221 taataagtta gcagcttcac ttttttgctt gtttgtccga aacggtctac ccttccttct
    14281 ctttgttcat gtttggtagg attccagctg agatcgtagt gaattacagc atcaaaataa
    14341 ttctgcaggt taattccttc agaaagacaa tcggtacaaa ccaaaacccg ttttttatgt
    14401 ttggaaagtt caattattct tttttctctc tcttctggag gtaaaacacc cgtaacagca
    14461 gaaatttcta catcttgggg taaacgctgt ttgagctggt cggtaagata ttcagcagtt
    14521 tgaataaagc ggcaaaagat gataggtttg aagccttctt ttagataggt ctttagtgga
    14581 ttaattatat ttagcagttt atagtccagc tctccaaaaa gggattctgc ttcttgggca
    14641 agagctctta gttttttgtt actgtaagaa ttttcttctt cggaggaaat actaccggga
    14701 acaatatcac tgctttctgt ggcatcgcga tcataagtat catacacttg gcgacgaccc
    14761 aattcatcgg cttcgaaggg actatcagtt tcctgagtag cattgcggtt attcattgtt
    14821 tttaaagcag cagcaggact ggaagctaaa gctcgtaata aagaaagtgc agaccaccat
    14881 ctaatccttc tgtgaagatc acccccttcc ttatcctgga caatttctct ggcataattc
    14941 agtgctttgc taaaaagctg acgatagggt tcggagagtt tataactaag ttcttcaata
    15001 atagcttcag gaaaagaagt atcttccaga taatgttcaa tatcagctct gcgacgctgg
    15061 ataaaatgtt tggcaattgt cttacgataa ttttcgtttt cagttcctgt tagatcatca
    15121 ggataagcgg taaattcact gttcaacaaa cttaataggg aacgaaaggc attcacattt
    15181 cctgaatggg gagttgccgt taccaaaata agatgacgca aggggtcttt acttaatgcg
    15241 ttaaccagtt catagcgttg atgtctgcta cttgtgctaa cactatcaaa actggcagta
    15301 tgagcttcgt ctataataat catttctgga gctgtgcgga taaattcatc tttatgacga
    15361 ccgcttttaa tataatcgat ggaaactacc gtaaagggat agacctcaaa gacggattca
    15421 ttttgggcac aattcttttc caattgacgc actgtgcttg ataatactaa ttctgcatca
    15481 atatgaaatt tatccaataa ttcgctttgc cattgttcag ctatatgagg gggagttaaa
    15541 acacaaattc ttgatatttc acccctatcc aaaagttctc gggctattaa acaggcttca
    15601 atggttttac caacacctac atcatcggca atcaaaattc taacgggatc aagctttaaa
    15661 gccatcagta agggtaataa ttgataagga cgggggttaa cagccaatct gccaaaactt
    15721 ctaaatggac ctgcgctgtt tctaaaccct aatctgagag catttcgcaa gagattgcag
    15781 gattgataat cgcccaattc attaggattg ggcaaactga aagaagcggg atgaacttct
    15841 tctaatttgg ttagaatacc ggtaatttcg tcatcactgc ctcctaaggg tttaacaatt
    15901 agaagttcag aggttgattc tggttgaaca acccattccc tacctctaac agaaacaagt
    15961 gaaccgatag cataatcttc aggaatatca tttgaagatt tgtctggttg aagttgttcc
    16021 actttttctc cttttcaaga tgatataggt aatttatcta taaccggttt tatggtgtta
    16081 gttataaacc ggaacatatt cattttctcg ttccgaaaat ctccggatgc tgatcaatta
    16141 tagtttgcca gtcttgatta taaaggaatc ggattacttt ccagccaaga tcatcaagac
    16201 gagcatcaac ttctctgtct ttttctatat tattggaaaa atcgtgaacc tggccatcaa
    16261 tgtaaatagc aacatatata tcagggtaag taaaatcggg gcgtgtttgt gctttttcaa
    16321 ttaactgctg ggcgtgaagg ggcaggatat agttattatt atagatgaaa tccagccact
    16381 tactttccaa ctcggaacca cacaaatttc gcaattgatg gtaaagctca aggcgagtaa
    16441 cttcactgga ggatctctta aaatcagcat taagcatctc cttgagatgg attataatag
    16501 attttcggtc taaaaggaga tgatcgagct gattcttata gcttaaaaga caatcataac
    16561 aggctgcttc acatctttcg ctggcatttt tcagcatttc caaatcggtg aaactatccg
    16621 gatcataatg acatatttca agcgctttcc tgatagtttt acgaaagttt tgttcatcca
    16681 tcaatcgttt tagaactcct gcaccacctt ctgcagattc ataaaataaa atcgaatgtc
    16741 tcacattcct gtttggtaaa ggttctgctg ccagttcgtt atcctcaata tcaaaaacct
    16801 gttgaatagc attctttaaa gcagattgta aagaagccat aacagcaggc tctgcgttaa
    16861 actggggttc aaaaagaaga cagtttttag tgtcgctaac ataagggcag accaatattc
    16921 ttctgttgcc taaaggcgaa tcgtcctcat tatcagtatc aggatttctt tcccaaaaac
    16981 cgcgttcact atccagccag aaacctaatt gcctacctct atggttcttt ttaaaaccca
    17041 tattgatacg ccagatatca gctgaatctc cataagttag ttttgctaaa agctcatttt
    17101 cagaatataa ttccgtttgg aaacaggata gtttacctcc ctttttggca aagcggatag
    17161 tagtcctcat ttcatatccc attcgtaacc tttcttcttc gtcacagcta atttgttctc
    17221 ttcttttagc aacaacattc tgcattcgga aaagattagt taaaggcgaa tcaagttgag
    17281 ctttacaatt ttcacaaata tcattttcat atccatttag atatccgcat tcggcgcata
    17341 acttcataga agctaaagag ggtagattat tattatctct ggtaggaggc ataataactt
    17401 gagatattat gtatttagaa ccttcatggt agataagaga gcggggtcca aattccgtga
    17461 tagctaaaaa tctgggtcta ttaagatatt cgtcttcgcc tttaactgta ccacgagcag
    17521 gtatataagc agataaaggc aatctgggaa agttatatcc aggtaaaaaa ccttctgaag
    17581 caaaatagcg ataactataa aaatcagatt gaaaagcacc ctctattgaa gttaacaaat
    17641 ttagctgagt ttctgcttct tttctaagct gtttagcttg ttttctatca gcagctgatc
    17701 tgctaacatc taaaataata ctattttgta attgctgctg atttaaagca gcccgataga
    17761 ggtctctcca acgttcacaa gcctgttcaa actcgttggg aatttgctta attacatctg
    17821 taagccagtc gttattgaac caaatggtac tttgtagata attttggact gtagcaatca
    17881 cactttctgc tcgttcaaaa gccctgtttt gcaactcaga gttattcaag gtagatttta
    17941 cagaaggtaa aagctctaat ttttccgtac taatatcaat aatattgttt aaagctctac
    18001 ccaaacttag ttttgcttca gacaaccaaa tggcattgat atgagaatgt aacaggtttt
    18061 cattagcaat atctatctga ggtgtggaaa caactccgga aaccattttt tcgggatgct
    18121 tatagaaata ttggtcgtga ggacttcctg tgctacagta acttaaaatt aaggcaggtt
    18181 gtccactgcg tcctgctcta ccactacgct gagcataatt tgccggtgtg ggaggaatat
    18241 tacgcatatt aacaacattt agccaggaaa tatcaattcc taattccatt gtgggagaac
    18301 agtataaaat tggtaaagtc ccttctcgga aagcagtttc tctttttttg cgctcttcat
    18361 aactaacctg agcagtatgt tcgcgagcgt atattttatg ggtagttagg gcaatagtag
    18421 aataatattc tttgaaaaat tcatttaccg gctgaccttc caaagattca gtaggttgat
    18481 gaagagggtc gtaataacca gttttcccgt caccttgttt ccagataata gaatcggcaa
    18541 ccaactgata tccgttattt ttgtcatcaa ccttttctat tattccatag cgcgttaacc
    18601 tatctatcag gttttcgatg atagttttgg tttgttgtaa atctatcgta gtcttataat
    18661 ccttaaaggt ggtggctctg cgtaaatatt ggccataaga actacgaggg gtaacatata
    18721 aattacttcc ccgatttcgc gcattgctat taggatcgag ataaagtata gaagcatagg
    18781 ttaaatttcg ggattgtgtg ctttcatcaa aaccccaagg aggaataaga cgttgatggc
    18841 tgttttgtaa cataatatcc tgaaaacggg gatcaagata atttactttt atcgttaaag
    18901 atctgcgcat tagttccaac agagttttca ggatattata tcttgcttgg tgatcacagg
    18961 atgacaaagc aggatggcaa ttttgccatt cttcttctga atacgctaat tcttccagag
    19021 ccaagtaatc aattttcagc aggcctgttt gttctaaatt gggtgctgta attctccatc
    19081 ctcttcgcag atcataatac agacgataac ctaaaacatc acagagagcg cgttcagtat
    19141 aaatacgggc agaatctctg tccgcaagag ggtttgaggc atattcttca taatttagat
    19201 taagacaatc aaatactttt tgggctaatt cgctatgacg aattccatcg ctacctgcat
    19261 taactaaagc ttgatacaaa gctccacgca ataaaccaat ttgaataaaa tcattaaaat
    19321 gaccagcttg aagagaggca tcctgacggt tatcggtaaa gcttaataat tttttggctt
    19381 tatcttccat atccatttgt tttatattgc ggataacgga aagggaaaga atcgtggttg
    19441 ctgaagctct tccttccgtt cccaaagcgg ttaacttggg aaaatcactg cgttgggtag
    19501 catcaaaaga cacaccacag gaagggcaaa atctaaaagg agttttaaaa aagtacatta
    19561 tcctgccagc ggggttttca atgccatcgg gtctaacaga tactttttcg ggcatatcat
    19621 atttacgcgc agcaatgata tttccattga tgtctttcca ttcaggtgga atcatagcag
    19681 ttaaatcaca atcaggatta tccggaagat aaagaaaacc agcatcctct tcatatagat
    19741 agatatcgta caattcacgg ggagtgtata gagtttcgtt atccttattt agcgttctat
    19801 gcactgtata aaaatcctga ccgcaaaaac ggcagaaggt aagtggtaat agaactttta
    19861 atttgtttgt atcgggaaca tataattgct tttgcattgt gaaatagcga gaatcattat
    19921 cttccagaga agcatataca gtttgacctc tgctgataaa ctgatgaagt cgaaaagcga
    19981 agacaggaaa gctattaata ggattaagtg tctgaaagcc taacatcaac attttttgta
    20041 tggcacgaac acaaagttca ttttctttat cagttaagtt atgcaataac cgagctccgc
    20101 ctttgggtcc ggttatgctg attggctcag aacgcactaa aattcctgtt ttaggatcgg
    20161 ttctaattcc aaaattgctt tctatccagg aagctaaatg gctgtgtttg atttgttcaa
    20221 attccagctt aggaacattt ataacctccg agatttcttt ttttaacagc tcgagttcat
    20281 ctcgattagt aaaatcgtat tcagctgtaa tcctttccaa agattcacca ataacatttc
    20341 cttgattgaa atcaatccca aaaagaagag aagaaacctt ggcaatttcc tgcttttgag
    20401 tttctatatc gccacttgat aaagtagcag aagtaccaac aaaaataaca tcctttgttc
    20461 ccgtagcttc ttttactctt ctaattaaca ttgcaacatc cgcaccttgc ctgccacgat
    20521 atgtatgaag ctcatccaaa actaaaaact tcagattttt agaagcactc accaaaggag
    20581 tttcatctgg ccgagttaaa ataagttcca acattacata gttagtaaga ataatatcag
    20641 gtggattggc cagaatttct tgtctttctg cgtcattttc ctggcctgta taacatctga
    20701 aacttatatt gtttgctgaa gatttatgat ggggattgat gaatttttcc agttcttgtt
    20761 tctgactatt ggctaaggca ttcatcggat agatgataat tgctttgatg ccctttccag
    20821 aaccagtttg aagtatatga tttactattg gaattatgta tgccatactt ttgcctgaac
    20881 cagtacctgt agttaagata taactttcat tggcgacagc ttttttgatt gcgtcggatt
    20941 ggtgtttata caattgtaag ggcttactac caatttcctt ttcatttctc aggcaaaaga
    21001 tcttttcaca atcaggatgc aagaaacctt gggaaaccaa ttcttttaca gattctccta
    21061 aggtaaaaaa aggatttatc tgcacaaggg gatcaggcca caataaaccc ttattaattt
    21121 tgtctaagac ataattgctg gctttactat cctttatatt gatgaaacca ttaacaaatg
    21181 aactatactc ttcaattatc ttgtctcgga gttcaaagat attcattgct atttctccta
    21241 ttgcttaaag cttatttggt aaaaaaaaca tattaacttg tcaagggaaa tctctcaaca
    21301 taaaaaaagc agacacatat ttcaaattac gaactgactt ttacaaatag cttgggtgtt
    21361 cagaatgcta agttacgggc agagatgatg agtcctttgt gcctgtagaa ttcaccaaaa
    21421 aggtaaaata atcgaggcac aaagggcttc tcacttgtta agcagggggc ttatttcata
    21481 ataataaagc gtccggcttt gctgttacta ttttgcaagc ggtaataata gatcccgttt
    21541 ggcaaatttg tatctggtac aaagctgatt tcattctctc cgctatgcag gttagtttct
    21601 ttcaatgttt gcactttctg gcctttcaca ttgtatattt caatggtggc agatcctaca
    21661 tttttagggt tagagaggtg aaacacagca gaacttttaa ccgggttagg gtaaaaccct
    21721 ttaataatat agggggatag agtattagta tcatcaataa taggggtgct tgcagttata
    21781 tgaaagggaa cattgtaact gcctatatgg ggactattta cagcaattcg gaaatcagcc
    21841 gttccggaag agccaatcat caggtttaga tgaatggagg tagcttctcc ggcagccaga
    21901 gtaaaaggac gatcaattga accaggatag caattccctt cttcgtcaca atagttaaaa
    21961 taccaatctg caggggcatt atctacttga atggacattg tgtaattgtc tgctgctccc
    22021 aaattgaata accatatagg ggaagaaaga taattggtgt tcggttcaac agttacagaa
    22081 gttttccaat ccatagctac tctgaaatta tattcaggca aaggaagaga agatacgcat
    22141 tggactatgg ttttatcgga aagctgtata aagattaatc cccagagatt actgaccgac
    22201 caatcagggt tcaaatcgaa ggttcgattg aaattaaggg tggttccagc gccgcttaag
    22261 gatgcaatat catcgtaaag tacacctcta aggacattag tttcgctacc aacattatct
    22321 tccagcagat agtataccag cttggcatta gaaattacag cagtaggaga aagcattgta
    22381 acattggcac ttacagctcc tctccctaaa ctgaaatccg taatatcaat ttttacaggc
    22441 gaagcagaaa agcgagtttg agatagggca ttaagataat tcgtgccatc tgcagttcct
    22501 tctcctgctc cctctataga tattaaacca ttaaagatta cattgggtaa accgatcact
    22561 ccataataac taaatctctc ttctatttct gcattgctca aagacccgct ttcagtgaat
    22621 aaacgggctg tgataaattc gccattatgg gtttcggaat gcactgcctg taaaccctgc
    22681 caagcaacaa gacaatttgg acaagacaga gaaccaaaat cttcggcaat agtggtaaca
    22741 ggcataaagt tgggttgggc aaataaaatg cccgcaacca ggaaagccag tagtaaatta
    22801 atatatttca ttttatgctc cttgtgagtt atataataac cgattatggg aagttgacaa
    22861 ccaaaaatat gggaattaat atcacgcgtt ctttctatca aacaggtctt agggatgtat
    22921 ggaagctata tcggcagtga tgattaggaa cagatgcaat tacctgatag ttcgataacc
    22981 ctgtgttcat cccaaaccta ttctatctgc gtgaaatatc ctatttaaaa gcgggtgtta
    23041 agctctatcc gtaaaccttc aaaaggtgca acataacggc agactccatt tctacatact
    23101 ttcccgccag cttcttttcc gccaaaaagt attatatcgc tatgttcaaa aagaggatat
    23161 tttatttctg cggaaggata atagcggcta tccagcattt cgcttatttc ttcccaactg
    23221 ctttgcacac aaacagatag agcgagttta cctaaggcga agtcagtttg cagtttaggt
    23281 tcatagtgtt cactttctgt tatctgtttt tgtttggaga tctttttata ttctgcttga
    23341 atcttcacag ggatattaaa cagataaaaa cttgattgca gagcagggat taattcctgc
    23401 tgccaagcag agttaatatc atcgattttt tcaatttgac tatatgaggc gagcaacgaa
    23461 tatgaatttc ccaggtattc caaggcaaaa taggcatcgt tcattttttt atctttatcg
    23521 ctattgaacg cttcagcata atctacagtg aaattaagat tatcagtaaa attaacagtt
    23581 ccaaaaccct gatagccaat ttcgtcttcc ccagaagccg aggcatcgga aagggtttca
    23641 ttatgataat ttgccatagg aagatcattt aaacgatatt gaaatttatc atactgttta
    23701 taagctcctc ctattgtaat aaaaccgatc aaataactgc cattcacata tattgccttg
    23761 ccgttcttag ttccgattcc gggttgatgg tataattcgc tgaaagccgt ttctgcttgt
    23821 aaatcaagat tatgggcaga ataattcagt cttccggcaa agacggtgcg ctgattatag
    23881 atattggtag ccagcagatt acgaaaagcc atagcggaag cgccaaaaga tagacccttg
    23941 aaataaggag tttgcaaatc agctccgtaa gctagatcat atctacctat gatattagga
    24001 ttttcgatag ctccgtaaat cgctttaaac ttaaaatcat cattatagct gaataaaaaa
    24061 ctctctaagc gattgtctat atcaaattct aagtctttat agctacggaa aacaattcca
    24121 ttaccaaaac tctcttctgt gattcctgcg tatattttac aggcatcttt ttcgtaggat
    24181 gcatataatt cctgccaacc aagctccaaa cgatttgcat ccagttcatc catcaattct
    24241 gtctgttcag ttgaatattt gggtagttca gcaatgaatt tcatcccgaa actgaagttt
    24301 ctatagccta ggttgaagcc gaaagcatct ttaaaataag tatgcaacga atcatctact
    24361 gtgcgataga taaattgagc ttcattggag ccatttaccc agagggtatt ttgagcgcct
    24421 aacaaggtaa aactgactaa gatcaggatt aataaaaagt gtttcattct cccttccatt
    24481 gtttatattt ttctctcaca gatttcactg attacacaga tatagattta gaaatatgga
    24541 tttcaagaat ttttagttat tgatgatatt gttaacaata actaattaac ttgaacatcc
    24601 attcctttca tctgggtagt cggagctcgc tgaagctccg tgtttttttt tgcggaggaa
    24661 cggcgtcctc ggtctacaca tctaaatcct taaaatcctt gtttcgaaaa ccatatctgt
    24721 cgtcccgctg ggactatttt ttattcgttc ttcttgtcgc cgggttaaaa cccgtcgtta
    24781 gtatgtatcg t
//
LOCUS       DAOSTH010000018        23916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_2292_length_23916_cov_12.507984, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000018 DAOSTH010000000
VERSION     DAOSTH010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 23916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23916
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2292_length_23916_cov_12.507984"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLB65_03995"
     CDS             <1..505
                     /locus_tag="PLB65_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77721.1"
                     /translation="YSTSMKIKSEPNSKHITVSLMDNKLNDVLEHLRKQLSDFNLMPF
                     TEYESWTPISIYSAILKTMKVPKTKIKELCDLIPSGVNSLFEAVLINTEFSCFSSEDY
                     VCRLSAVIADKLISTFREEKSAKQKYVLIPKRMSIPIVRDNDGNARCQYDFNFINQIC
                     LISLEFT"
     gene            837..1010
                     /locus_tag="PLB65_04000"
     CDS             837..1010
                     /locus_tag="PLB65_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77722.1"
                     /translation="MNTVLTVKGIGTNLIEIINATKPKKVQEKNIRLLIADKTIPVIP
                     PILVDIYAADWKG"
     gene            1172..4693
                     /gene="nifJ"
                     /locus_tag="PLB65_04005"
     CDS             1172..4693
                     /gene="nifJ"
                     /locus_tag="PLB65_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HOQ77723.1"
                     /translation="MAKQTKATMDGNTAAAHIAYAFAEVAAIYPITPSSTMGELVDAW
                     ASEGRKNINDTKVDVIEMQSEAGAAGAVHGSLSAGAVTTTFTASQGLMLMLPNMHKIA
                     GEMLPTVFYVTARSLAAQSLSIFGDHSDIMSARNTGFAFIACNSVQEVMDLGLVAQLA
                     TMKTRIPFVVFFDGFRTSHELQKIDVIDYETIAELNDPALVEEFRKNALSPDRPRMKV
                     GQEGPDVYFQGRETSNLYYAKAPEIIAETMEQVAAKTGRSYKLFDYAGHPEAEDIIVA
                     MGSSCETIQETIEWLNANRGMKLGLVKVHVYRPFNVENFLAAIPKSVKRIAVLDRTKE
                     PGSIGEPLYLDVVSAVKNRPDTYVIGGRYGLSSKEFTPSMVLAVYKHLANKGFHGFTV
                     GIDDDVSHLSLPIEEVINTVPKGTISCKFWGLGSDGTVGANKNSIKIIGDHTDMYAQG
                     YFQYDSKKSGGITRSHLRFGKTPIHSQYLVAQEDFVACHNQAFIGRFDLLGGIKENGV
                     FLLNSNWSRDEAFYNLTRPMQETIINKNIRFYNIDGLKIAEEVGLGGRINTTMQTAFF
                     LISGVLERNEAIKLIKESIKKTYTRKGEDIVQMNLEAVDRVNEALVEIEVPKTLPDKY
                     MPLKKLVPEDADDFILNVIEPVMREQGDQVKVSQMPLDGYVETGTAKLEKRRVAPSVP
                     HWLPEKCIQCNQCSFVCPHAAIRAKLVKEEDLTNKPSSFNTLKATGAPGYEYKVQVYI
                     DDCQSCKVCVNECPKGALVMSPIDEEREKGEQSNYEYFETLPTDVLANFKEATVKGSQ
                     FKQPLLEFSGACAGCGETPYIKLLTQLFGDRMIIANATGCSSIWGGTFPTIPYCKNKD
                     GRGPAWANSLFEDNAEYGFGMRLAIRSHRKLLQKAMQELLDKNINEELKTALQYSLDN
                     WDKVDELAKANADKIRALLPKAIETACEGCKKLLKKVQELQDYLIDKSIWAIGGDGWA
                     YDIGYGGLDHTLALNQNINVFVLDTEVYSNTGGQASKSTPIGSIARFAESGKNTNKKD
                     LGMMFMSYGYVYVASIAMGANKNQALQAIKEAEAYPGPSIVIAYAPCINHGFDMSYSQ
                     KHQKLAVDAGYWLLYRYNPLNKLEGKNPLTLDSKEPTVSVQEFLESENRYARLQKIFP
                     ERAKNLNESAAVFFKERYETYKKLTQQ"
     gene            complement(4926..6203)
                     /locus_tag="PLB65_04010"
     CDS             complement(4926..6203)
                     /locus_tag="PLB65_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HOQ77724.1"
                     /translation="MLLMIIALLVFIVTYLCIITEWVNKMLAALIGGFVIIVLGVVDQ
                     TIAFSAIDWNVIFFLIGMMLTISVMRETGMFMYIAIKTAKIAKGSPLKIMALMFMATA
                     LISAILGSVTTIMILIPIVLLISEELKITPVPFIITMVVASNMGGAATMIGDPPNILI
                     ASATKFTFVDFVLNLTPIILIIVAFSLGLIWLLYRGKMRVSNERRAKIMEYNDKNLIT
                     NHKLLWITLSVVGLMLLAFIFQKPLHLENATIAMAAGLILVFVGSRKKVETIILNDID
                     WITIFFFIGLFMIVEGLVHTGFIDLLAQGVISVTNGEPKTTSMVILWLSGIFSAFVDN
                     IPFVAAMIPMIERIGEVITDPVQIKPLWWALSLGTCLGGNGTLVGASANIVAVGIANR
                     NGYKISFMDYTKIGVLFALESVILSSLYIWIRY"
     gene            complement(6207..7106)
                     /locus_tag="PLB65_04015"
     CDS             complement(6207..7106)
                     /locus_tag="PLB65_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOQ77725.1"
                     /translation="MLLASFLEPRNILFENRVLTKEQLYQILVDRLCSQYKLPICGSK
                     LMELILQRDEEVSPAYSTGIAIPHIRMENFNDTLIAMAFLQNPLDYNGIKVSWVVLII
                     TDKTSSKLYLNIVAALLKLSNDKEAVADLHNTGDGHSVIQYLKKKEVEVKKDVTIADI
                     MVQKPIAVHPENTLRELINLMCMYRVAGMPVVDATGKYIGEVNILNLLKVGIPDFVMM
                     LDDLSFLASYEPLENLLEKQDLLLVNDIMATDEKTLSPETSIIEAVFLMFTNKKRYLS
                     VVKEGKLVGLVTAMDILTKVIAV"
     gene            7474..9195
                     /gene="recJ"
                     /locus_tag="PLB65_04020"
     CDS             7474..9195
                     /gene="recJ"
                     /locus_tag="PLB65_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOQ77726.1"
                     /translation="MNKNWLIPAPPGKELLSDIEKLGMELKLPRLVAELLYRKGIKTS
                     SEAQEFFNPSMENLYDPFLFPDMEKAVQRILKAIDNKEKITIYGDYDVDGTTATALLY
                     LGLKRIGANIDFYIPHRMIDGYGLSLTSLDTLRENGSSLIISVDCGVNAIEEINAINS
                     MGMEIIITDHHNPKDELPPAFAIINPKLVGMKYPYEHLAGVGVAYKLLIAIYMKLGID
                     TAENKLKYMDLVAVGTIADIVPLNSENRIFASIGLQHLIEKKNLGLNALIQISGLNQK
                     NLDTSDIVFGIAPRINAAGRMGSASVSVELLISTDEAKSMELAEIIEHQNSLRQQEDQ
                     KTFQEACEIIEKKYKDMQQTSCMVISSDDWHPGVIGIVASKLVEKYYRPVIMISFKDG
                     FGSGSGRSVADFDLFEALKQTEHNLHSFGGHKYAVGLTVYQEYLDRFENELTRYVADH
                     LRLEQIQPPLQIDAEIELYDINTMLLDALDLFAPYGPDNTRPVFMTKNVTIASYPYNV
                     GRNHLKLKVVKDGINFDLIGYNMGDYLPLLRKAGKLNIAYTLEYNRFGNNVTIQGKLR
                     DLQIMKD"
     gene            9200..10036
                     /locus_tag="PLB65_04025"
     CDS             9200..10036
                     /locus_tag="PLB65_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77727.1"
                     /translation="MKQLLFLALILAYAIMLFTLFACSGINPQPPMNIEQFSLINTIP
                     LGMQTDKILYHSQSNTLYAMSVKNQEIYIFQDGELKNVLGGLGSSKVNFQYLADMALG
                     TDNSLYVLDSSTKSVKKFISDGKPSGSIELKGSVQPKCLCIYQEQEMFIFDSASGEII
                     NYSGIDGTEQNRFGRFQLQQINSLSCNHDYLIAYDKDNNLSHIFSLLGQFVKDEPGQV
                     VYDMFNNGICYNEGMLKCLNSPAYLPLTEDKVLIFIHNNILALHQGEEVRILKIVYSQ
                     VE"
     gene            10037..11527
                     /gene="lnt"
                     /locus_tag="PLB65_04030"
     CDS             10037..11527
                     /gene="lnt"
                     /locus_tag="PLB65_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOQ77728.1"
                     /translation="MPPLLWVLFSALLLSLSRLPLHLGWLAFTGWIPLLYVLESRKVK
                     LWQAAMIFSLVYIGVVMYWIAGVTFLGLIGICIFYFLFYWLCFYAINRISRVSGYLFY
                     PGFILVMLSFEYLQNFGETRFPWFNNAYSLSDYTVLIQAADLGGVILISLLILVINVL
                     LYEVLIKKNRRGLLYLVGIFILWIGYGIYCLHSLPMQKQEPKIAIMQPSIPQEEKWDE
                     EAYQQILHRYDTLCAKAAEDSTKLIIFPEAAIPVYLMYDNYAKTYLRELMQKYNLEIF
                     TGFPHFTLAPDNHINDELYYNAAALFKPDGSISELYYKNILVPVGERMLWLNLFPFLW
                     KLQFGQANWEFGNGPAYYQTDSLEFSPSICYELAFAEFHQKMAIRKDKDTGSYKKTDF
                     LVNITNDAWFGTSYGPWLHSVMAKFRAVENRIQIYRSANTGISLIVDPFGRILTKAGL
                     FEITNLYAPLIICPKIPVYRYIYWYPWIFVILVAGLFIYSFLKKEA"
     gene            11541..11936
                     /locus_tag="PLB65_04035"
     CDS             11541..11936
                     /locus_tag="PLB65_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOQ77729.1"
                     /translation="MKKYYYTIGEVSNLLNVKPHVIRYWESEFPWIKPKKNSGRIRKY
                     SEKQVLLLRKIYDLLYNQRYTIEGARQKIKQERTTIQEIAKDALDESSTSINEVSGMK
                     PEIKEVLLDLKNDLTQIQQTCKKYTQDKK"
     gene            11933..12790
                     /locus_tag="PLB65_04040"
     CDS             11933..12790
                     /locus_tag="PLB65_04040"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOQ77730.1"
                     /translation="MNFQDIILTLQDYWAKKGCCLIQPYDLEMGAGTFHPSTFFGTLG
                     KKPCAIAYPQPCRRPKDGRYGENPNRFQHYYQFQVIIKPIPDDIQNLYLKSLAAIGIE
                     IEKHDIRFVEDDWESPTLGAWGLGWEVWLDGMEISQFTYFQQVGGIDVFPVSVELTYG
                     LERLAMYIQNVDDYRMLAWNETTKYAELFYEREKEYSAYNFELANTKLLFQLFQDYET
                     ECNALLSKQLVYPAYDMLLHCSHTFNLLEARGVISVTERASYISRIRNMARACANNYL
                     NKINNNQEE"
     gene            12793..13452
                     /locus_tag="PLB65_04045"
     CDS             12793..13452
                     /locus_tag="PLB65_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77731.1"
                     /translation="MNKDFSNVMSTLTGIQDSLLQVAVYTDFQPQCVIILEEDPPDLL
                     ESLDSLKNYTIKRKLRFPLLINRNFAESSLDSYPLEFLDIVSSNYENLFVKKDLLKTL
                     KFDPVNLRLQMEREIKSKWLLTRLSYLEMKTDSKKMANIIALTLTNILPVLKGICYLK
                     DGSAPPQYDAAVERATNITDIEFDTIFSWIANKKVSLENVKQYLAILESLMQYLEMID
                     K"
     gene            13449..14225
                     /locus_tag="PLB65_04050"
     CDS             13449..14225
                     /locus_tag="PLB65_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HOQ77732.1"
                     /translation="MIYSDTYLGNSNFPILAFAIHNGHYLPEELLQGCGISEDTRLRE
                     EDPYTSIFAETFPNRIVVQTSRFAVDLNRSPEKAVYQKPEDAWGLQVRKHPVPEKLNE
                     MLIKAYEAFYRTAHYQIERLLHNNPKLIILDLHSYNHRRGGPNAAPDPQNENPDIILG
                     RNNLPESKYQTIEKLRLLIDNKPCVDSKLDCRCDVKFSGGYFSRWINSTFGDKVICVA
                     VEFKKIFMDEWTGELNLSACLQLKEIFHNAVLEWMEQVNG"
     gene            complement(14440..15939)
                     /locus_tag="PLB65_04055"
     CDS             complement(14440..15939)
                     /locus_tag="PLB65_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77733.1"
                     /translation="MKVSVCFTFLLLVCCLFLFAEVEEIPELDTENLEDNWLNLLYSE
                     IEDGKYNFGLNNRLKYSENCLTNFRLQQKELCLNLNLYFKESSELDANFQLSRLPSKN
                     NRSEIYFGSYRPAFGLGTVLKKSGEKTLFTVNRPAHPVYYSPFGAATIVRINSVSLFF
                     MGSGRKRNASLKENKINSLYKTIHSESPVVQENILAGGLEYQMNNSQFGILVYEQNYD
                     RNFVDSLMAKKMQVYAFSTKMNRENITIATELAYLEKDLALQTTSQFSYHNIAQEISY
                     AYRQGNQLPVYAAKPYLLSSKGESQEINWNLNYQPHKIVYLDLRYALIRKNNCLKSPV
                     WNSRSIINLSLKPSSTAINLQLTRLDKEIISENDSSYITTLPVHYRFRLKVRQELNEN
                     MDFTLLCRYHYEDKMELHKNSFYWENAFRYSQKRVAISTGIKTWQTLNMLIIPDEYTE
                     NPSGTIATASDENIFFIVLSYKGNLFQCKTEMQQSWLNGNRSLYFSISI"
     gene            complement(15936..16715)
                     /locus_tag="PLB65_04060"
     CDS             complement(15936..16715)
                     /locus_tag="PLB65_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77734.1"
                     /translation="MRFKVGREYIVVITTMLILLCYSSYLISVTTENSASASALSGVT
                     ILSDSPADYILSPIIGSSGICLSWQQPYSIDEINIYGLHSAFKVKPIIVATGIAYLAH
                     PDYRYQDEYLALSAEWSCFHFGLTQHLVYEKIEEQSWFTWDNDLALALQKNKIKTEIR
                     CNHLRTEDIAVTLSAAVIANESSSFSSTYSWRKNDRNNYCLATSFAIAKPLLLQCSWQ
                     NEPARFGVGMKVMLGKMSVMYAVRTHTELGLTHIVDLGSAW"
     gene            complement(16751..17854)
                     /gene="ugpC"
                     /locus_tag="PLB65_04065"
     CDS             complement(16751..17854)
                     /gene="ugpC"
                     /locus_tag="PLB65_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HOQ77735.1"
                     /translation="MAKVTIKNLNKYFENGFHAVKDVSFTAEDKEFIVLVGPSGCGKT
                     TILRMIAGLEDISSGEIYIGDTLVNDVPPKDRDIAMVFQNYALYPHMTVFDNMAFALK
                     MRKMPKEEIKIKVSNSAKLLGIESMLKRKPGQLSGGQRQRVALGRAIVRNPKVFLFDE
                     PLSNLDAKLRVAMRAEIVKLHKTIGNTMIYVTHDQVEAMTMADRIAVMKDGVIQQIDS
                     PLKIYNDPANVFVAGFIGSPAINMTTGKIARTNGALHFVSDDYLLKLTPRQEEILKNY
                     EDKAIIMGIRPEDIYDSRFDALADFPQKIITKVDFVEPLGNEYHVVLTTEHNEFIARF
                     DPKELPKMGQDLSITFDMTKAHFFDPTSEANLY"
     gene            complement(17928..19625)
                     /locus_tag="PLB65_04070"
     CDS             complement(17928..19625)
                     /locus_tag="PLB65_04070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOQ77736.1"
                     /translation="MMNPINSHSNLYDISLFTSIEQEMRSTLKKLIGDFIIADDSTRS
                     EIEEQVIEIVVSAYSENCENPLYCEYVGLIIQYLLDYFLQIRTTHESEFYHFLNFLSH
                     FPITQDSPYYSYIAKSAKEKQEKEQEYIELFNQLQLLYILTENGDYANAAEFLRILEP
                     KVDINNLGLWILIQLSKAGVLRYQNQHRELLRTHLDLIIEIYNRDGSDCAINLIIRWL
                     ISIPWHKQGIIKKYLLLRMYDYLEEQKSLNSAMVLYELFTLEDRFVSSAEKIEYQRVL
                     IKYPASVLNVQQLHSLYFFAGYYNCGVLSNFKDSIQNYQYSNYFLHKSWDSLLSLSHF
                     MRDCLDPEHYYSAMPFLESRIRELSNQASLQNNAYVESLQANFYKIEELYEKVGELSL
                     TDSLTGLRNRRYLEGNIFQMIVLAARHNVPVCFSMIDIDFFKLINDNYGHLVGDYVLK
                     EIAAILSAEFRKSDVIVRYGGDEFLIILFDSDPNLSYSMMEELRNKIEVHTFEYQNLK
                     INITISIGIACDFHKSNKPTNLSKCISFADQACYAAKTNGRNRVEIYKSEKQPENNC"
     gene            complement(19606..19926)
                     /locus_tag="PLB65_04075"
     CDS             complement(19606..19926)
                     /locus_tag="PLB65_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HOQ77737.1"
                     /translation="MKTKDKKRSPQFRFFFWGAIVLLVIWILFLANNSFLNTWKLKHR
                     VEQLEKETAALKAQNDSLAMENNRLKTDPEAAEKAARENFGLTKPDETVFRFVPAKDD
                     EPDK"
     gene            complement(19926..21050)
                     /locus_tag="PLB65_04080"
     CDS             complement(19926..21050)
                     /locus_tag="PLB65_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HOQ77738.1"
                     /translation="MHRPTVQIRKIDNYHLPEVEEAVANFFSSIKSPKMHKAKRVLIK
                     PNALGAYAPERAITTHPIVLEAIIRYFLKRDKEIWIGDSPGGSVAFDKVWQTCGFASL
                     ADKYPVKVVNFSTTNFRTLSYQNLQIKVSEALFQCGIVINVGKYKTHQLTSFTGALKN
                     LYGFVPGLIKSEYHRANPDTTSFANLLVALYASIRNRITYSIIDGITGMDGNGPSAGK
                     PRDFGLLFGSTSIPALDYTASRIMGFRLKDVPYLSPALHLEGILPSHIDIPISFRNYQ
                     LTDADIKTVRLTQKTLKYVPGIARKAFRKVYYFYPEVSDRCRRCGICVNSCPVKAISW
                     QKNANPYIHREQCIKCLCCHELCPYQAIDIKKSFIAKLGI"
     gene            complement(21050..22207)
                     /locus_tag="PLB65_04085"
     CDS             complement(21050..22207)
                     /locus_tag="PLB65_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77739.1"
                     /translation="MLRHIILTVMFCLLFGFLAAQVDSLYLPQFANPFYDTFSRNYNG
                     TTAAGRGYTGVAILGGMDNALLNPASVLPGLGTILVEMNVKPSMEADGYPMYANYVSK
                     VPIGMLAINATIASKFSAGLLYSNPKSITLEDFSFYTNQGQNMIQRFPTYNLHQATAM
                     VAYHRGPLHLGLNLHNQIHYIDDPIFLRSYDRVKEFAYNLRVQPGLIYEFSNMNMGLT
                     MMPSSKFDWDLKYASYEAVLPLWIAGGVQFATAKYGIAAEGEWEQTSDICDSFEDRYT
                     FKAGLEKYSGDLAYRLGYHFSSNVYSGMIPLAVNYANPDTSNAWDSVPELIYIKDNAQ
                     HSITAGISYYSKYGSLNLALMQVVAGDIKKTQVNISLGFYLKKIIHKRNPL"
     gene            complement(22214..23488)
                     /locus_tag="PLB65_04090"
     CDS             complement(22214..23488)
                     /locus_tag="PLB65_04090"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOQ77740.1"
                     /translation="MASTLVLGCMWGDEGKAKIVDYLAASADFVVRFQGGSNAGHTIV
                     YGNQKYVLHTIPSGILYPDTTCLIGAGVLVDPTLIKEEIHNLEKANIDFSKRLLIDYR
                     AGVVLPLHKQLDMENETKLKKSKIGTTGKGIGPAYSDLTARIGIRLEDLAYPEYLSER
                     LKQLYTYHHKKLTDAELTKQIAELQDSWKFLKKYTGDVESILNDIANIKDKSILFEGA
                     QGTLLDLNFGTYPYVTSSRVMTDAAGIGTGFSARRIDKVLGIYKAYTTRVGEGPFPTE
                     IFTDIADRIRTAGNEYGSTTGRPRRIGWFDGVAARYSARINALDNAVITCLDVLQGLD
                     ELKICSAYKYKGKLYRSMKLHPSQFDKIEPVYESFEGWDADICNCKNKSELPKAARDY
                     LEAIEDFLETRICLVSVGREREQTFTIKERKR"
BASE COUNT         7618 a         4423 c         4358 g         7517 t
ORIGIN      
        1 ttattcaacc tctatgaaga tcaaatccga acccaatagc aagcatataa cggtttcttt
       61 gatggataac aagctaaacg atgtattgga gcatcttcgt aagcaacttt cggactttaa
      121 cttaatgccc tttactgagt atgaatcttg gacgcccatc agtatctatt ctgctatttt
      181 aaagacgatg aaagtgccca aaacgaagat caaagaactc tgcgatttga tcccttccgg
      241 ggtcaattca ctttttgaag ccgtcttgat taacacagaa ttttcctgct tttccagtga
      301 ggactatgtt tgtcggctat ctgccgtcat agcggataaa ctgattagca cattcagaga
      361 agaaaaatca gcaaaacaaa agtatgtttt aatccccaaa agaatgtcca ttcctattgt
      421 tcgagataat gatgggaacg caagatgtca gtatgatttc aactttatta accagatttg
      481 ccttatcagt ttggagttta cataataagt ttgaaggcag taatccaact ggatataaag
      541 gtgcttagaa ttgcacaata cttgcttttg ttgttacaaa gctaagcaat tcttgatttt
      601 gaataaagaa gataaaagag atggtagtgt ctaaaaggtt ggcataaaag agaaacggga
      661 aagccaattg gatttgtgca acaaatttat taggaatccc atattatatt ttcgaccact
      721 taaagtataa tatggaaatt catattatat ttttaaccaa ccaaagtata atatgggttt
      781 tattgaagca gattcaccgc aggaagatct ggctacttta catacaaact tttgtaatga
      841 acacagtttt gacagtaaag ggtattggta ccaatctaat agagattata aatgccacta
      901 agccaaaaaa ggtacaagaa aagaatatca ggttattgat tgccgataag accattcctg
      961 ttatccctcc aattttggtt gatatatatg cagctgattg gaaaggataa aaagaagtag
     1021 tactttttac ctttaccacc cactttttat aaaaacccaa cctttaccac ccaactgttt
     1081 acacaattta ggttgacgaa atacccttgt tcaattatga tgcaagaaaa agaaagatat
     1141 tgtattaata tgaaatataa ggagttatcc aatggcaaaa caaaccaaag ccactatgga
     1201 tggtaatact gcagcggcgc atattgctta tgcatttgct gaagtagctg ccatttatcc
     1261 aattaccccc tcttccacaa tgggtgagct tgttgatgct tgggcatcag aaggcagaaa
     1321 aaatattaac gacaccaaag tagatgttat tgaaatgcaa agtgaagctg gagctgcagg
     1381 agcagttcat ggttctctct cagcaggtgc ggttacaaca acttttactg cttcgcaagg
     1441 tttaatgttg atgttaccca atatgcataa gattgccggt gagatgttac caactgtttt
     1501 ttatgtaaca gccaggtctc tggcagctca atctctatct atttttggcg atcatagtga
     1561 tataatgagt gctcgcaata caggttttgc ttttattgct tgtaatagtg ttcaggaagt
     1621 aatggatttg ggtttggtag cacaacttgc tacaatgaaa acccggatac cttttgtggt
     1681 cttttttgat ggtttcagaa ccagtcacga actgcaaaaa atagatgtta ttgattatga
     1741 aacgattgct gaattgaatg atcctgcttt agtagaagaa tttcgcaaaa atgctttaag
     1801 tcctgatagg ccacggatga aggttggtca ggaaggaccc gatgtatatt ttcagggtag
     1861 agaaaccagc aatttatatt atgccaaggc acctgaaatt atagctgaaa cgatggagca
     1921 ggtagctgcc aaaacaggac gtagctataa gctatttgat tatgccggtc atccggaagc
     1981 agaagacatc attgttgcta tgggaagcag ctgtgagact attcaggaaa ccattgaatg
     2041 gctgaacgcc aatcgcggca tgaaattagg tttagtaaaa gtacatgttt accgtccttt
     2101 taatgtggaa aactttttag cggcaattcc caaaagcgta aagagaattg ctgttttaga
     2161 cagaacgaaa gaacctggtt ctattggtga acctctttat ctggatgttg tgtctgcagt
     2221 gaaaaaccgt cctgatactt atgttatcgg aggccgatat ggtttatcca gtaaagaatt
     2281 taccccctct atggttcttg ctgtttataa gcacttggca aacaaaggat tccacggttt
     2341 tacagtaggt attgatgatg atgtatctca tctctcttta ccgattgagg aagttatcaa
     2401 tactgttccc aagggcacta tctcttgcaa attctggggt ttaggttcag atggaacagt
     2461 aggcgcgaac aaaaacagca tcaaaattat tggtgatcat accgatatgt atgcgcaggg
     2521 ctattttcaa tatgacagca aaaaaagcgg cggtatcact cgtagccatt tgcgttttgg
     2581 taaaacccct attcacagcc aatatctggt agctcaggag gactttgtag cctgccataa
     2641 tcaagcattt atcggacgct ttgatttact gggtggaata aaagaaaatg gtgtcttctt
     2701 gctaaattcc aattggagca gagatgaagc gttctataat ttaacccgtc cgatgcagga
     2761 aactataatc aataagaata tcagatttta caatattgat gggttaaaga ttgcagaaga
     2821 agtaggtttg gggggaagaa ttaatactac tatgcaaact gcgttcttcc ttatttcagg
     2881 tgttttagaa cgcaatgaag ccataaaatt aattaaagaa tccatcaaaa agacctacac
     2941 ccgtaaaggt gaagatattg ttcagatgaa tttggaagct gtagataggg tgaatgaggc
     3001 tttagtggaa attgaagttc cgaaaacctt accggataaa tatatgccac tgaagaaatt
     3061 ggtacccgaa gatgctgatg actttatctt aaatgtaatt gaacctgtaa tgcgcgagca
     3121 aggagatcaa gtaaaagtta gccaaatgcc tcttgatggt tatgtagaga ccggaactgc
     3181 caaactggaa aaaagaagag ttgcaccctc tgttccccat tggctgccgg aaaaatgtat
     3241 tcagtgtaat cagtgttcat ttgtttgtcc ccatgctgca attcgggcta aattagtaaa
     3301 agaagaagat ttaactaata aaccttccag cttcaatact ttaaaagcaa ccggtgcacc
     3361 cggctatgaa tataaggttc aagtttatat tgatgattgc caaagctgta aggtttgcgt
     3421 aaatgaatgt cccaaaggcg ctttggtaat gagcccaatt gatgaagagc gggagaaggg
     3481 tgaacaatca aattacgaat acttcgaaac cctaccaacc gatgttcttg ccaactttaa
     3541 agaagcaact gtaaaaggta gccagtttaa acaaccttta ttagaattct caggtgcctg
     3601 tgcaggttgt ggagaaacac cttatatcaa gctattaact cagctctttg gtgatagaat
     3661 gattattgct aacgccactg gctgttccag tatctggggt ggaacttttc ccacaattcc
     3721 ttattgcaaa aataaggacg gcagaggtcc tgcctgggcg aatagcttat ttgaagataa
     3781 tgcagaatat ggctttggaa tgcgcttggc aattcgttca caccgtaagc ttttacaaaa
     3841 agcaatgcag gaactgttgg ataagaatat taacgaagag ctgaaaactg cacttcaata
     3901 ttcacttgat aattgggata aagtggatga gctggcaaaa gccaatgccg ataaaattag
     3961 agctctcctt cctaaggcaa tagaaactgc ctgtgagggc tgtaaaaaac tgctaaaaaa
     4021 agttcaggaa cttcaggatt acctcattga taaatcaatt tgggcaatcg gaggtgacgg
     4081 atgggcttat gatataggtt atggaggttt agatcatacc ctggctttaa atcagaacat
     4141 caatgtattc gtgttggata cggaagttta ttccaatact ggaggtcagg caagtaaatc
     4201 tactccgatt ggttcgatag ctcgttttgc cgaatctggt aaaaacacaa ataaaaaaga
     4261 tctgggaatg atgtttatga gctatggtta tgtgtatgtt gcttctattg ctatgggtgc
     4321 taataaaaat caagcactgc aagcaattaa agaggcagaa gcatatcccg gaccttctat
     4381 tgttattgcc tatgcgccat gtatcaatca tggtttcgat atgagctata gccagaaaca
     4441 tcaaaaatta gctgtggatg caggttactg gctattatat cgctataatc cacttaacaa
     4501 attggaaggt aaaaatccgc taaccttgga tagtaaagaa ccaaccgtat cggtgcagga
     4561 atttttggaa tccgaaaatc gctatgcaag attgcaaaag atctttccgg aacgagctaa
     4621 aaaccttaat gaaagtgcag cagtgttctt taaagaaaga tacgaaactt ataaaaaatt
     4681 aactcaacaa taaaattaca aggttgactt cctcgtcatt cattggaaac ctaagttctg
     4741 gttaaagtgc aagttagcca taactgagaa gttaggttga aagtgaaatg acgaggatgt
     4801 ctttattcca ggaaagttga tgtcctcgtc aacccactat atacatacta tcaagcttat
     4861 atgaaagtta gcagcagcca cctaatttgg ctgtgagagg aacgacagaa tgtctttatt
     4921 gcagattaat accttatcca gatatacaaa gaagagagga ttacagattc aagagcaaag
     4981 agaacgccta tcttagtata atccataaag cttattttat aaccgttacg atttgcaatt
     5041 cccacagcta caatattcgc tgaggcacca actaatgttc catttcctcc caaacaggtt
     5101 cctaaggata gagcccacca aagaggttta atttgaacag ggtctgtaat aacttctcct
     5161 atgcgttcga tcataggtat catagctgct acaaaaggta tattatcaac aaaggcagaa
     5221 aaaatgccag acagccaaag aatgaccata gaagtagttt tcggttctcc atttgttaca
     5281 gatatcacac cttgggctaa taaatcaata aagccggtat gcaccaaacc ctctacaatc
     5341 ataaaaaggc caatgaagaa aaagatggta atccaatcta tatcattaag gataattgtt
     5401 tccacctttt tcctactccc cacaaaaaca agtataagac ctgcagccat agcgatagta
     5461 gcgttttcca ggtgcaaagg tttttggaaa ataaaggcca gtagcattaa acctacaacg
     5521 ctaagagtaa tccacaaaag tttatggttt gttattaaat tcttatcgtt atattccatt
     5581 atcttggctc gccgttcatt acttacacgc attttccctc tgtataaaag ccaaattaaa
     5641 cccaggctga aagctacaat tatcaagatt ataggagtta aattcaaaac aaaatccaca
     5701 aaggtaaatt ttgtggcgct ggcgattaga atattaggtg gatcaccaat cattgttgct
     5761 gctccaccca tattagaagc aacaaccata gtgatgataa aaggaacggg agttattttc
     5821 agttcttccg aaattaacaa cacaatcgga atcaaaatca ttatcgtcgt aacactgcct
     5881 aaaatagcag agataagcgc agtagccata aacatcaaag ccataatttt cagtggactt
     5941 cccttggcta tttttgctgt cttaatagcg atatacataa acattcccgt ttctcgcatc
     6001 acggaaatag taagcatcat tcctatcaga aagaatatta cattccagtc aattgcagag
     6061 aaggcaatgg tttggtctac cactcccaaa acaatgataa caaaaccacc aatcagcgca
     6121 gcaagcattt tgtttaccca ctccgtgatg atgcaaagat aagtaacgat aaagaccaat
     6181 aaagcaataa tcattaacaa cattatttac accgctataa cctttgttaa aatatccatt
     6241 gccgtaacca agcctacaag ttttccctct tttactacgg atagatatcg ttttttatta
     6301 gtgaacatta agaacaccgc ttcgattata gatgtttctg gggaaagagt tttctcgtct
     6361 gtagccatta tatcgttaac taatagcagg tcttgttttt ctaaaaggtt ttccaagggt
     6421 tcgtaagaag caagaaagct taaatcatcc agcatcataa caaaatcagg aatcccaact
     6481 ttaagtagat ttaggatatt tacttctcct atatattttc ctgttgcatc cactacaggc
     6541 ataccagcca ctctgtacat acacattaaa ttgatcagtt cgcgcaaagt gttttctggg
     6601 tgaacagcta ttggcttttg aaccataata tcggcaatag taacatcttt tttcacttcc
     6661 acttctttct tttttagata ttggataacg ctatgtccat cacctgtgtt atgaagatca
     6721 gctacagctt ctttatcatt agataacttt agtaaagcag ccactatatt cagatagagc
     6781 ttggaacttg ttttatcagt tataataagc accacccaag agactttaat tccattataa
     6841 tccaaggggt tctgcagaaa agccatagct atcaaagtat cgttgaaatt ttccatccta
     6901 atatgaggga tagctattcc tgtagaataa gctggagaaa cttcttcatc ccgctgtaat
     6961 attagctcca taagtttaga cccgcaaata ggtaatttat attgactgca aaggcggtca
     7021 actaaaatct ggtacagctg ttctttagtt aataccctat tttcaaacag gatattacgg
     7081 ggctctagaa agcttgctaa caacatttag cacctcataa tatatataat ttgtgtcctt
     7141 tttaatacca tattgatttt agtcaatagt tattatgtag aaacctgata attggtgtaa
     7201 ttagaattat actttacatt tgtaatgcag gtttcgcctt tgctacttga tttgttaacc
     7261 tattaatctg tgaaatggaa actgaacaag agtgttttct ttttgaagtg cgagggaaaa
     7321 ataattcttt acacgatata gctttcaatt attatagtat ttagcggata cgatacgcat
     7381 tattttctta agaaaatgtt gtaatatgct gtaaatctgt tagtttcgta ccagaataag
     7441 atacaaataa ataaaaaaga taaaggcaaa aaaatgaaca agaactggtt aatacctgca
     7501 cctccaggta aagaactact ttccgatatt gaaaagttgg gaatggaatt aaagctaccg
     7561 cgtttggtag ctgaattatt gtatcgtaaa gggattaaga cctcttcaga ggcacaagaa
     7621 ttctttaatc cttctatgga aaatttatat gatccttttc tctttccgga tatggaaaaa
     7681 gccgtgcaac ggattttgaa agccatagat aacaaagaga agataacgat ttatggcgat
     7741 tatgatgttg atggcacaac tgctactgcc ttattatatt taggtttgaa acgcattggt
     7801 gccaatattg atttttatat cccccatcga atgatagatg gttatggttt atccttaacc
     7861 agtttagata ctttaaggga aaatggttct tcactcatta ttagtgtaga ttgtggagtg
     7921 aatgctattg aagagatcaa cgccataaat tctatgggga tggaaataat aattacagat
     7981 catcacaacc ccaaagatga gcttcctcct gcttttgcta ttattaatcc caaactagtt
     8041 ggaatgaaat atccttatga acatcttgct ggagttggag ttgcttacaa attgctgata
     8101 gccatatata tgaaactggg aattgatact gcagaaaaca agcttaagta tatggatctt
     8161 gtagcagttg gaactatagc cgatattgtt cctctcaatt ccgaaaatcg aatttttgct
     8221 tcaattgggc ttcagcactt aattgagaaa aagaacctgg gcttaaatgc tttgattcaa
     8281 atttccggct taaatcaaaa aaatctggat accagcgata tagtgtttgg tattgcaccc
     8341 agaatcaatg cagccggcag aatgggaagt gcctctgttt ctgtagaatt gttgatttct
     8401 accgatgagg caaaaagtat ggaactggct gaaattattg aacatcaaaa ctctttacgc
     8461 cagcaggaag atcaaaagac ctttcaggag gcctgtgaga ttattgaaaa aaaatacaaa
     8521 gatatgcagc agacatcttg tatggttatt tcttccgatg attggcatcc tggggtaatt
     8581 ggaattgtcg cttccaaact ggtggaaaaa tattatcgac cagtaataat gatttcattc
     8641 aaagatggtt ttggtagtgg ttctggacgt agtgtagccg attttgatct ttttgaggca
     8701 ctgaaacaaa cagagcataa tctacacagt tttggtgggc ataaatatgc cgtaggttta
     8761 actgtatatc aagaatacct tgatcgtttt gaaaatgaat taaccagata tgtggctgat
     8821 catttacgct tagaacaaat tcagccccca ctacagattg atgcggaaat tgagctatac
     8881 gatataaaca ctatgctgct tgatgcctta gatctttttg caccttatgg tccagataat
     8941 actcgtccag tttttatgac gaagaatgtt accattgcca gttatcctta taatgtaggg
     9001 cgtaatcatc tgaaactaaa agtagttaag gatggtatta attttgattt gattggttat
     9061 aatatgggtg attatctacc tctactaaga aaagctggta agctgaatat tgcctatact
     9121 cttgagtata atcgctttgg taataatgta accattcagg gtaagttaag agatttacaa
     9181 attatgaagg attagcctta tgaaacagct tctattttta gcccttattt tggcttatgc
     9241 tattatgcta tttacccttt ttgcctgttc tggaattaac ccccagcctc caatgaatat
     9301 tgaacagttc agtttgatca atacgatccc cctgggtatg caaacggata aaattctgta
     9361 tcattcacaa agcaatacgc tctatgcaat gagtgtaaaa aatcaggaaa tatatatctt
     9421 tcaggatgga gaactgaaaa atgttttagg tggtttaggc agttctaaag taaactttca
     9481 atatctggct gatatggctt tgggaactga taatagttta tatgtgttag attcctctac
     9541 caagagtgtt aagaaattta tttcggacgg taaaccttca ggcagtattg agttaaaagg
     9601 aagcgttcaa ccaaaatgcc tatgcatata tcaagaacaa gaaatgttca ttttcgattc
     9661 tgcaagtggg gaaataatta actattcagg aattgatggc accgaacaaa atcgtttcgg
     9721 cagatttcaa ttgcagcaga tcaattcact ctcctgcaat catgattatt taatcgccta
     9781 cgataaagat aacaatctta gccatatctt ttccttgtta ggccaatttg taaaagatga
     9841 acccgggcag gttgtttatg atatgttcaa taacggcatt tgttataatg aaggaatgtt
     9901 gaagtgtttg aacagtcccg cctatttacc cttaaccgaa gataaagtac taatatttat
     9961 tcataataat atcttagcgt tacaccaagg tgaagaagtc cgcattttaa aaatagtcta
    10021 ttcgcaggtg gaataaatgc ctcctttact ttgggtttta ttttcggctt tattgctttc
    10081 gctatcgcgt ttacctttac atttaggttg gctggcattt acaggttgga tacccttact
    10141 ttatgtatta gagagcagaa aagtgaagtt atggcaagca gcgatgattt tttccctcgt
    10201 ttatattggc gttgttatgt attggatagc gggagtaact tttttggggt taattggtat
    10261 ctgtatcttt tattttctat tttactggct atgcttctat gcaattaata gaattagtag
    10321 agtatctgga tatctttttt acccggggtt tattttggta atgcttagtt ttgaatatct
    10381 ccaaaatttt ggtgaaacca gatttccctg gtttaataat gcttattcgc tctccgatta
    10441 tactgtttta attcaggcag cagatttggg aggagtaatt ctgatttctc ttctgatttt
    10501 ggtgattaat gttctgcttt atgaagtttt aattaaaaaa aatcggagag gactacttta
    10561 tcttgtcggc atctttattc tctggattgg ttatggaatt tattgtttac attcattacc
    10621 tatgcaaaag caggaaccca aaatagctat tatgcaacct tctattcctc aagaagaaaa
    10681 atgggatgaa gaagcttatc agcaaatttt gcatcgttat gatactcttt gtgctaaagc
    10741 agccgaagat tctaccaaac taatcatttt cccagaagca gcaatacctg tctatttaat
    10801 gtatgataat tatgccaaaa cctatttaag agagctgatg cagaaataca atctggagat
    10861 ctttactgga ttcccgcatt ttactttagc tcccgataat cacattaacg atgaattata
    10921 ttacaatgcc gcagcccttt tcaaacccga tggatccatt tctgaattgt actataagaa
    10981 tatcttggtg cctgtaggag agcgaatgtt atggctgaat ttatttccgt ttctctggaa
    11041 actgcaattt ggccaagcca actgggaatt tggaaatggg cctgcttact atcaaacgga
    11101 cagtttagaa ttttctccgt ccatttgtta cgagcttgct tttgcagagt tccatcaaaa
    11161 aatggctatt aggaaagata aggacaccgg cagctataaa aaaactgatt tcttagttaa
    11221 tattactaat gatgcctggt ttggcacttc ctacggaccc tggctgcatt ctgttatggc
    11281 aaaatttcgt gctgtggaaa acagaatcca aatttatcgc agtgctaata caggtatttc
    11341 tttaattgta gacccctttg gtcgcatttt aactaaagca ggtctctttg aaataactaa
    11401 cctgtatgca ccgcttatta tttgccccaa aataccagtt taccgttata tatattggta
    11461 tccgtggatt tttgtaattc tggtggcagg acttttcatc tacagcttcc ttaaaaaaga
    11521 agcttaaaaa gtgaggtctt atgaaaaaat attactatac tatcggggaa gtgagtaatc
    11581 tattaaatgt taagccacat gttatccgtt attgggaaag tgaatttccc tggataaaac
    11641 ctaaaaagaa ttcaggacgc attcgcaaat atagtgaaaa acaggtacta ttattgcgta
    11701 aaatttatga tttattgtat aatcagcgct atacaattga aggcgcaagg caaaaaatta
    11761 aacaggaacg aactactatt caggaaattg ccaaagatgc tcttgacgaa tcttccactt
    11821 cgataaacga agtatctggt atgaaacctg aaattaaaga agtactactg gatttgaaaa
    11881 atgatttaac ccagatccag cagacctgta aaaaatatac acaggataaa aaatgaactt
    11941 tcaagatatc attcttacac tgcaagatta ttgggcaaaa aagggttgct gcctaattca
    12001 gccctacgat ttagaaatgg gagcagggac ctttcatcct tccacttttt ttggaacttt
    12061 agggaaaaaa ccctgtgcta ttgcttatcc gcaaccatgt cgcagaccta aagacggtcg
    12121 ttatggagaa aatcctaatc gctttcaaca ctattatcag tttcaagtga ttatcaaacc
    12181 cattcctgat gatattcaaa atctatattt aaaaagttta gctgctattg gtatcgagat
    12241 agaaaaacac gatatcagat ttgttgaaga cgattgggag tcaccaactt taggcgcttg
    12301 gggtttgggt tgggaagttt ggttggatgg aatggaaatt tctcaattta cttattttca
    12361 acaggttgga ggcatagatg tctttcctgt tagtgttgaa cttacttatg gtttagaacg
    12421 gctagctatg tacattcaaa atgtagatga ttatagaatg ctggcttgga atgaaaccac
    12481 aaaatatgca gaactttttt atgagcggga aaaagagtat agtgcataca acttcgaatt
    12541 ggcaaatacc aaattgttgt ttcagctttt tcaggattat gaaacagagt gtaatgcttt
    12601 gctaagtaaa caactggttt accctgccta tgatatgtta cttcactgtt cccatacttt
    12661 taatttatta gaagcaagag gagtgataag tgttacagaa cgtgcttcct atatctcccg
    12721 catcagaaat atggctcgcg cctgtgcaaa caattatttg aacaaaatta acaataacca
    12781 agaggaatag ctatgaataa agatttctct aatgtaatga gcactttaac tgggattcag
    12841 gattctcttt tacaggtggc tgtttatacg gattttcaac ctcaatgtgt aatcattctg
    12901 gaagaagacc ctccagacct tttggaatca cttgattcct taaagaatta taccataaag
    12961 agaaaattgc gtttcccttt attgatcaac cgtaattttg cggaaagctc tttggattca
    13021 taccctttgg aatttctgga tatcgtttcc agcaattacg agaatctttt cgttaaaaaa
    13081 gatctcctaa aaacacttaa atttgatcca gtaaacctgc gtttacaaat ggaacgcgaa
    13141 attaaaagca aatggttgct tactcgtttg tcctatctgg aaatgaaaac cgactccaaa
    13201 aagatggcta atattattgc cttaactctc acaaacattc tgccagtatt aaaagggatt
    13261 tgttatttga aagatggctc tgcccctccc caatatgatg ccgctgtaga aagagcaaca
    13321 aatattaccg atatcgagtt tgatacaata ttttcttgga tagctaacaa gaaggtaagc
    13381 ttagaaaatg ttaaacagta tcttgccatt ttagaaagcc ttatgcagta cttggaaatg
    13441 atagataaat gatttacagc gatacctatc tgggaaattc taattttcct atccttgctt
    13501 ttgccatcca taatggccat tatttgccag aagaacttct ccagggttgt ggaatttccg
    13561 aagataccag attacgagaa gaagacccat atacaagtat tttcgcagaa acctttccaa
    13621 atagaattgt tgttcaaacc agtcgctttg ccgtagattt gaaccgttct ccagaaaaag
    13681 ctgtttacca aaaaccagaa gatgcctggg gcttacaagt gagaaaacat cctgtgccag
    13741 aaaaacttaa tgaaatgctt attaaagctt atgaagcatt ttatagaact gcccactatc
    13801 aaattgaacg cttactacat aacaacccta aactgattat tttagattta catagctata
    13861 atcatcgcag aggtggacca aatgcagctc cagaccctca aaacgaaaat cccgatatta
    13921 ttttgggaag aaataatctg ccagagagca aatatcaaac aatagaaaaa ttgcgtctac
    13981 tgatcgataa caaaccctgt gtagattcaa aactggattg ccgctgtgat gttaaattct
    14041 caggtggata tttttctcgt tggataaaca gtacttttgg tgataaagta atctgcgttg
    14101 ctgtagaatt taaaaaaatc tttatggatg aatggacagg agaattaaac ttatccgcct
    14161 gcttgcagtt gaaagagata tttcataatg ctgttttaga atggatggaa caggttaatg
    14221 gatgaataag tgataggttt ttaatgaggt tataaatagt tatatctttt ttgtgtgatg
    14281 gacttatgca tttagtgcta aagtaatcac taccaacatc gtaacccact aaatctctat
    14341 aagctaaaga aaatataact gcaggaaaca catttttctc ctttagccac actccatata
    14401 attaatggaa aggccgttgc tgatttatat cttcttgtat tatatactaa tagaaaaata
    14461 gagagagcgg ttgccattta gccaagattg ttgcatttcc gttttacact gaaacaaatt
    14521 ccctttatag cttagcacaa taaaaaatat gttctcatca cttgctgtgg ctatagttcc
    14581 cgagggattt tctgtatatt catcaggtat aataagcata tttaaggtct gccaagtttt
    14641 tattcctgta gaaatagcaa ctcgcttttg gctgtagcga aaggcgtttt cccaatagaa
    14701 gctattttta tgaagttcca ttttatcttc atagtggtaa cggcagagca aggtaaaatc
    14761 catattttcg ttcagttcct gcctaacctt tagacgaaaa cgataatgga cagggagggt
    14821 agtaatataa ctactatcgt tttctgatat aatttcttta tccaatctgg tcaattgcag
    14881 atttatcgct gttgaagaag gtttcaaaga aaggtttatt atactgcggg aattccaaac
    14941 tggacttttc aggcaattat ttttcctgat tagggcatag cgcaagtcca aatacactat
    15001 tttatgtggc tgataattca gattccagtt aatttcttgg ctttctccct tactacttaa
    15061 aagatatggt ttggcagcat aaactggtaa ttgatttccc tggcgatagg catagctaat
    15121 ttcttgagca atattatgat agctgaattg tgaagttgtc tgcaaggcga ggtctttttc
    15181 cagataagca agctcagtag ctatcgtaat attttcccta ttcattttgg tagaaaaggc
    15241 ataaacttgc attttttttg ccataagtga atctacaaag tttctatcgt agttttgttc
    15301 atataccaaa atgccaaatt ggctattatt catttgatat tccaatcccc cagcaaggat
    15361 attctcttgc actacagggc tttcagaatg gattgtttta tacagagagt ttattttatt
    15421 ctcttttaag gaagcattgc gttttcgtcc cgaacccata aaaaataagg atacactatt
    15481 tatccgcaca atcgttgccg ctccaaaagg tgaatagtat actggatgag caggtctatt
    15541 aacagtaaaa agagtttttt ctccgctttt ttttaatact gttcccaatc cgaaagctgg
    15601 acgataggaa ccgaaatata tttcggaacg gttatttttt gagggtaaac gggacagctg
    15661 aaaattagca tctaattctg aactttcttt aaaataaagg tttagattca agcatagttc
    15721 tttttgctgt aaccgaaaat tagtaaggca attctctgaa tattttaatc tgttattcag
    15781 tccaaagtta tatttcccgt cctctatttc tgaatataat agattcaacc agttgtcttc
    15841 aagattttct gtatctaatt ctggaatttc ttcaacttca gcaaatagga aaagacaaca
    15901 aactaacagt aggaaagtga aacaaactga aaccttcacc aagcagaacc caaatccaca
    15961 atatgcgtta agcccaattc tgtatgagtt ctaactgcat acatcacact cattttacct
    16021 agcataacct tcatcccaac tccaaaacga gctggttcat tttgccaaga acattgaagt
    16081 aataagggtt ttgctatggc aaaagaagtt gccaggcagt agttatttct gtcgtttttg
    16141 cgccaagaat atgtagatga aaaactggaa gattcatttg ctatcacagc tgccgataaa
    16201 gttacagcta tgtcttcggt tctaagatgg ttacagcgta tctccgtttt aattttattt
    16261 ttctgcaaag caagtgctaa atcgttatcc caggtaaacc aactttgctc ttctatcttt
    16321 tcatagacca gatgttgagt tagcccaaaa tgaaaacaag accactcagc acttaaagcc
    16381 aaatattcat cttgataccg ataatccgga tgtgccaaat atgcaatacc ggttgccaca
    16441 ataatgggct ttaccttgaa ggcactatgc aaaccgtata tattgatttc gtcgatacta
    16501 tagggttgtt gccagctaag gcaaattcca ctggaaccta taattggaga taatatataa
    16561 tcagcaggtg aatcagaaag aatagttacc ccagataagg cacttgcaga agcagaattt
    16621 tccgtagtta ccgatatcaa atagctactg taacaaagca ggatgagcat tgtagtaata
    16681 accacaatat actctctccc tactttgaac ctcataggat aaatcctttg gttacataaa
    16741 ggttgctagc ttaatatagg tttgcttcgc ttgttggatc aaagaaatga gctttagtca
    16801 tatcaaaagt aatacttaag tcttgtccca ttttcggtaa ttcttttgga tcaaagcgtg
    16861 cgataaattc attatgttca gttgttaaaa ccacatgata ttcgtttccc aatggctcaa
    16921 caaaatctac cttggtaata attttttggg ggaaatctgc taaggcatca aaccttgaat
    16981 cgtatatatc ttcaggtcgg atacccataa taatagcttt atcttcataa ttttttaaga
    17041 tctcttcctg acggggagta agttttaaca aataatcatc gcttacaaaa tgcagagcac
    17101 cattagtgcg agcaattttc cctgtagtca tattaatagc aggactacca ataaaaccag
    17161 caacaaatac attagctgga tcgttatata ttttcagggg agaatcaatt tgctgaataa
    17221 ctccatcttt cataacagca atacgatctg ccatcgtcat agcttctact tgatcatgtg
    17281 taacataaat catagtatta ccgattgttt tatgcaattt cacaatttct gctctcatag
    17341 ctactctcaa ttttgcatct agattagaaa ggggctcatc aaaaaggaaa actttcggat
    17401 tacgaacaat ggctcttcct aaggctactc gctgtctttg tccacctgaa agttgacctg
    17461 gttttctttt cagcatactc tctatgccca ataattttgc tgagttactc acttttatct
    17521 tgatttcttc tttgggcatt tttctcattt ttagggcaaa agccatattg tcaaacaccg
    17581 tcatatgagg ataaagagca tagttttgga agaccattgc tatgtctcta tccttgggag
    17641 gaacatcatt aactaaggta tctccaatat agatttcacc agaagagata tcttccaaac
    17701 cggcaatcat tcttagaata gttgttttcc cacaacctga agggccaact aaaactataa
    17761 attccttatc ttcggcagta aaagaaacat cttttacggc atgaaagcca ttttcaaaat
    17821 acttattcag attctttatt gttactttcg ccatttctaa actcctatat aattacttat
    17881 tgaaagcaat tggtatatat gtcaagtggg aaatcataga tatgcatcta acaattgttc
    17941 tcaggttgtt tttctgattt ataaatttca acgcggttcc ggccattcgt tttagccgca
    18001 taacaggcct gatctgcaaa ggagatacat ttagaaagat tagtgggttt attgctttta
    18061 tgaaaatcac aagcaatacc aatactgatc gtaatattaa tcttcagatt ttgatattca
    18121 aaagtgtgta cttcaatctt gttccgtagt tcttccatca tagaataact taaattagga
    18181 tcagaatcga acaaaataat taagaactca tctcctccat aacgcacaat tacatcactt
    18241 ttacgaaact cggcactaag gatagcagca atttctttta agacataatc accaaccaga
    18301 tgtccatagt tatcattaat caatttaaag aaatcgatat cgatcatact aaagcaaaca
    18361 gggacattat gtctggcagc taaaacgatc atttgaaaga tattaccttc caaatatcgt
    18421 ctattgcgta aacccgttaa gctatctgtt aatgaaagct ctccaacttt ttcatagagt
    18481 tcctcaattt tatagaaatt tgcctgcaag ctttccacat aagcattatt ctgtaaagat
    18541 gcctgattac ttagttctct gatgcggctt tctaaaaaag gcatagctga ataataatgt
    18601 tcaggatcaa gacaatctct cataaaatgc gataaactga gcaaactatc ccaactttta
    18661 tgcagaaagt aattggaata ttggtaattt tgaatcgaat ccttaaaatt ggaaagtacc
    18721 ccgcaattat agtatcctgc aaaaaaataa agcgaatgta gctgttgcac atttaataca
    18781 gaagccggat atttaattaa tacccgttga tattctatct tttctgcaga agaaacaaaa
    18841 cgatcttcta aagtaaaaag ttcatagagc accatcgcac tattcaaaga tttctgttct
    18901 tcgaggtaat cgtacattcg taataataag tactttttaa ttatgccctg cttatgccag
    18961 ggaatagaaa ttagccagcg gataattaaa tttatggcac aatcgctgcc atctctattg
    19021 taaatttcaa ttattaagtc caaatgtgtt ctaagcaatt ctctatgttg gttttgataa
    19081 cgcaaaacac ctgctttgga aagctgaata agaatccata atcccaaatt atttatatcc
    19141 actttcggtt ccagaattct taaaaattct gcggcatttg cataatctcc attttcggtt
    19201 aatatatata aaagctgaag ttgattgaag agttctatat attcttgttc tttttcctgt
    19261 ttttctttcg cactcttggc aatatatgaa taatagggtg aatcctgagt tatgggaaag
    19321 tggctcagga aattgagaaa atgataaaat tcactttcgt gggtggttct tatctgcaga
    19381 aaataatcca gcaaatattg aataatcaaa cctacatatt cacaatatag cggattctca
    19441 cagttctcag agtaggcaga aactactatc tctataactt gttcttcaat ttctgaacgg
    19501 gtactatcat cagctataat aaaatcacca attagctttt ttagcgtgga tcgcatttcc
    19561 tgttcaatac tggtgaaaag agatatatcg taaaggttgg aatgactatt tatcgggttc
    19621 atcatcttta gcaggaacaa aacggaagac ggtttcatct ggtttagtta gcccaaaatt
    19681 ttccctggca gctttttcag ctgcttcagg atctgttttc aagcggttat tttccatcgc
    19741 taaactatca ttttgagctt tcagggcagc agtttctttc tccaactgtt caaccctatg
    19801 cttcagtttc caagtgttta aaaaactgtt gttagctaaa aataaaatcc agataaccag
    19861 cagaactatt gctccccaaa agaaaaaacg gaactgtgga cttcgcttct tatcttttgt
    19921 tttcattata tccccaattt agctatgaat gatttcttga tatctattgc ttgataagga
    19981 cagagctcgt gacagcataa gcatttaata cattgctctc tatgaatata gggatttgca
    20041 ttcttctgcc agctgatagc cttcactgga cagctgttca cgcagatacc acatcttcta
    20101 cagcggtcac taacttcagg ataaaagtaa taaaccttcc gaaatgcttt acgagcaatg
    20161 cctggtacat atttcagggt tttctgagtt aaacgcacag ttttgatatc tgcatccgtt
    20221 agttgatagt tacgaaaaga aataggaata tcaatatgcg acggtaatat gccttctaaa
    20281 tgcaaagcag gacttaaata gggaacatct ttcagtctaa aacccataat ccgagaagca
    20341 gtataatcca aagcaggtat tgatgttgaa ccaaaaagaa gcccgaaatc tctgggttta
    20401 ccagctgaag gaccatttcc atccatacca gttataccgt cgattatgct ataagtaatt
    20461 ctgttacgga tacttgcata cagagctacc aacaaattgg caaaacttgt tgtatctgga
    20521 tttgctctat gatattcact tttaataagc ccaggcacaa atccgtaaag attcttcaga
    20581 gctcctgtaa aagaagttaa ttgatgagtt ttatacttgc ctacattaat cactatacca
    20641 cattggaata atgcttcgct aacttttatt tgcagattct gatagctgag tgtccgaaaa
    20701 ttggtagtgg aaaaatttac cacttttaca ggatatttat ctgcaagaga agcaaatcca
    20761 caggtctgcc acactttatc aaaagctaca gaacctcccg ggctatctcc aatccagatt
    20821 tccttatccc ttttcagaaa atagcggata attgcttcta aaacaatagg atgcgtagtg
    20881 attgcccgtt ctggagcata agcccctaag gcatttggtt ttattaaaac cctttttgcc
    20941 ttgtgcattt ttggggattt gatactggag aaaaaattag caaccgcttc ttctacctcg
    21001 ggcaaatggt aattatcaat tttgcggatt tgcactgtag gtctatgcat caaagtgggt
    21061 tccttttatg aatgattttt ttcagataga aaccaaggga tatatttacc tgagtttttt
    21121 ttatgtctcc agcaacaacc tgcatcaacg caagattcag agagccatat tttgaataat
    21181 agcttatacc tgccgtgata gaatgttggg cattatcctt gatgtatatt agttccggaa
    21241 cactatccca agcattggaa gtatcaggat tggcatagtt tactgccaag ggtatcatcc
    21301 cactatagac attggaacta aagtgataac ccaaacggta tgccagatcc ccactatatt
    21361 tttccaatcc agctttaaag gtataacggt cttcaaaact atcacaaata tcgctggttt
    21421 gttcccattc gccctctgcc gcaattccat acttcgccgt ggcaaattga acccctcccg
    21481 ctatccataa aggcaaaaca gcttcataac tggcatattt taaatcccag tcaaatttgg
    21541 aggaaggcat catagttaaa cccatattca tattgctaaa ttcatatatt aaaccgggtt
    21601 gaacacgcaa attatatgca aattctttta ctctatcata acttcgtaga aaaattggat
    21661 cgtctatata atgaatttga ttgtgtaaat tcaaccccag atgcagagga cccctatgat
    21721 aggccaccat tgctgtagct tgatgcagat tataagtagg aaagcgttgt atcatattct
    21781 gcccctgatt ggtatagaag gaaaaatcct ccagggtaat agatttggga ttgctatata
    21841 gcaatccagc tgaaaattta ctagcaattg tagcatttat tgccaacatc ccaataggaa
    21901 ccttggaaac ataattggca tacataggat atccatcagc ttccatagag ggctttacat
    21961 tcatctccac aagtatagtt ccaagtcctg gaagcacaga agcaggattc aaaagagcat
    22021 tatccatacc acccaaaata gcaacaccgg tataacctct tccagctgca gtagtgccat
    22081 tataatttct gctaaaggta tcataaaagg gattagcgaa ttgaggtaaa tataacgaat
    22141 ctacctgagc ggctaaaaaa ccgaataaaa gacaaaacat aacggtaagg ataatatggc
    22201 gcagcatttg cagttacctc tttctttctt ttattgtaaa ggtctgttcc ctctctcttc
    22261 ccacagaaac aagacagata cgggtttcca gaaagtcctc aattgcttcc agataatctc
    22321 tggctgcttt tggtaattca cttttatttt tacagttaca gatatctgca tcccaacctt
    22381 caaaactctc ataaacgggt tctattttat caaattggga aggatgtaac ttcatagacc
    22441 tgtatagctt gcctttatat ttataagcgc tacagatttt tagctcatct aatccttgca
    22501 gcacatccag acaggtgatt actgcattat ccaaagcatt gatgcgagct gaatatcttg
    22561 cggctactcc atcaaaccag ccaattcttc ttggtctgcc agtggtagag ccatattcat
    22621 taccagcagt tctaatcctg tctgcaatat ctgtaaatat ttctgtagga aaaggacctt
    22681 cacccactct tgtagtatat gctttataaa ttcccagaac cttatctatc cggcgagcag
    22741 aaaaaccagt tccaatgcca gcagcatctg tcataacccg agaagaagtt acataaggat
    22801 aggttccaaa atttaaatct aaaagtgttc cctgtgctcc ttcaaagaga attgatttat
    22861 ctttaatatt agctatatca tttaggatag attctacatc gccagtgtat tttttcaaga
    22921 atttccagga atcttgcaac tcagcaatct gctttgttaa ttcagcgtca gttagctttt
    22981 tatgatgata agtatacagt tgcttcagcc gttccgacaa atactccgga taagccaaat
    23041 cctctaatct tatcccaatt ctggctgtta gatcactgta agcaggacca attccttttc
    23101 ccgtagttcc aattttggat tttttcagct ttgtttcatt ttccatatcc agctgtttgt
    23161 gtaaagggag tacaactcct gcccggtaat ctatcagcaa acgcttgcta aaatctatgt
    23221 ttgctttctc caaattgtgt atttcttcct ttatcaaagt gggatcaact aatacaccag
    23281 ccccaattaa gcaggttgta tcaggatata aaatacctga aggtattgta tgcaacacat
    23341 atttctgatt gccatataca attgtgtggc ctgcattact accaccttga aaacgaacaa
    23401 caaaatctgc agaagctgct aaataatcta ctatctttgc tttgccttca tcaccccaca
    23461 tacatcccaa aactaatgtt gaagccattc tcaactcctt ttttagataa aatcatcttt
    23521 atctctattt ccatattctg tcaatctaat tttttcccct tattttcagc ggtaaaggta
    23581 aaaagtatcc cccctttatt atcctttctt atcatctaca tatatttatc aatcataatt
    23641 ggtagaattg caggaaaatt aacagcatat atagattgtt ttagccaaca ccttagtttt
    23701 cttgctggtt gaccatatac aacttgtcat ctacccaatc ttcactcctc ctcaacgatg
    23761 ggttgaggac aggaggagga ggagaggaag aggagttgtt tgtttgagtc atcttgaaaa
    23821 tagtggactt aagattttcg ccgggttaaa acccgtcgct taaatctgtc gtcccgctgg
    23881 gacttctttt aggcattctt tttatcgccg ggttaa
//
LOCUS       DAOSTH010000019        23219 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_2379_length_23219_cov_14.107235, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000019 DAOSTH010000000
VERSION     DAOSTH010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 23219)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23219)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23219
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2379_length_23219_cov_14.107235"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(421..1053)
                     /locus_tag="PLB65_04095"
     CDS             complement(421..1053)
                     /locus_tag="PLB65_04095"
                     /inference="COORDINATES: protein motif:HMM:NF017519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HOQ77741.1"
                     /translation="MSLIDLFSRGLTFSLILLTGSSAISSIRTANLFVESAFEFSQLS
                     SLELKDGDVILRKGNSILSELISRNFPAAEGMSHCGFIFKIQDQYQVIHTISKSISGI
                     DGIRMNTLEDFINEAKQNRICIIRYHKELNSAAMKKRCLVLLKQKTPFDNDFNLNDSS
                     ELYCSELLRKVYLEQSEPDFFKLRKIAGVEVIDFSTFFDTKLFTPIYKNY"
     gene            complement(1053..1391)
                     /locus_tag="PLB65_04100"
     CDS             complement(1053..1391)
                     /locus_tag="PLB65_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77742.1"
                     /translation="MKKLLIAISALALIFVLVGCAKGPESVAQKFIDAWENQDMNSME
                     KLSTPESKDMLGMFTMFKVKDATVGETKISGDSAIVNISYTTEEGNKDEFDMNMVKKD
                     GKWLVDIKGK"
     gene            2081..2713
                     /locus_tag="PLB65_04105"
     CDS             2081..2713
                     /locus_tag="PLB65_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77743.1"
                     /translation="MRKDNIEQGVKVNGVTLNYENEVTLSEGYDSNFLTIDGETLSLN
                     IKGSSEPKINLQLKYWEYKPGDAKFSLKKEKLSYETKSGKPVSLFSITGSIPKNLNLN
                     ISTGTGKVNLSDMIGEQTITIQAGTGSIIVSESKLKKLSAETGTGSIIIDKCNIDTTL
                     LESGTGSVHMKKSNIDMASVNTGTGNIILENSNINKQYFETGTGKVIINK"
     gene            complement(2929..4263)
                     /gene="ffh"
                     /locus_tag="PLB65_04110"
     CDS             complement(2929..4263)
                     /gene="ffh"
                     /locus_tag="PLB65_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HOQ77744.1"
                     /translation="MFDSLTERLDKIFRNLRGKGYLTEDNIKAGLREVRMALLEADVN
                     FRIVKNFISEVEKRALGAEVMKSLTPGQQVVKIVYEQLVNLMDTEGFQFNVYNNRLTK
                     VMMVGLQGSGKTTACAKLASFYRKKDTKLMMVACDVYRPAAIDQLKILGKQINVPVIS
                     EDTTNVLSIADSALSIADKEMVNLLIFDTAGRLHIDEPMMEEVARLKAHIKPDYIFFV
                     ADATTGQDAVNIAKEFYDKLAFDAVILTKMDGDARGGAALSIKAVTGKPVAFVGIGEK
                     ISDLEIFYPDRMASRILGMGDVLTLIEKAEESIDMEEEEKLARKMLKNQFTLNDFLKQ
                     LQSIKKMGSLESIIRMIPGLGHKLPADLNIDEKALKHIEAIIQSMTEKEREHPEIING
                     SRRLRIAKGCGRSVTEVNRLLKQFDEMKKMMKKVSTPKGMKHLERMMGGKLQ"
     gene            complement(4281..4934)
                     /gene="rpe"
                     /locus_tag="PLB65_04115"
     CDS             complement(4281..4934)
                     /gene="rpe"
                     /locus_tag="PLB65_04115"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOQ77745.1"
                     /translation="MQKTQIAPSILSADFGRLASEIAALETAGADILHLDIMDGHYVP
                     NLSFGFPVIKAIRRMTSLPLDIHLMVTNPDFHIPILLDMNVQWVSFHQETCYHSHRFI
                     QKLKDRGIKAGLALNPGTPLGTLEALLPDLDYILVMSVNPGYSAQEFIPSAIYKIWDL
                     FNLRKKNNYRYLIEVDGGITAANAKAIKEAGTDILVSASYIFSSDNYEQAIAALKNA"
     gene            complement(4916..5230)
                     /locus_tag="PLB65_04120"
     CDS             complement(4916..5230)
                     /locus_tag="PLB65_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HOQ77746.1"
                     /translation="MSFSSISKLSGQLVYQLGGDKYKNFIHIYLSWKKVVGELLASKS
                     YPIKIENKVLFVTVHNNTWMQELILIKDDIISKYKNIYEEEIADIVFLISSPKGKCKR
                     RK"
     gene            complement(5299..5874)
                     /locus_tag="PLB65_04125"
     CDS             complement(5299..5874)
                     /locus_tag="PLB65_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77747.1"
                     /translation="MSDLGYKIKQILSLIVFVAVLSLLGMISGRPIMILAYGVFFLVV
                     VAIMFYLTNKRQRHFEKVKESSPVFKKVFGIILVLLSLITPMVIIFKTNLVNLPESLP
                     IGTAIAIVGAVSIVFIALTLAAVYFINMRGKQVSNRVIGYILYVIAAMIPGLLMSRIE
                     KTTIGIGSVYYVALIVLILAYSGFGLITNRE"
     gene            complement(5893..6339)
                     /gene="rplI"
                     /locus_tag="PLB65_04130"
     CDS             complement(5893..6339)
                     /gene="rplI"
                     /locus_tag="PLB65_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOQ77748.1"
                     /translation="MKVIMLKTIEKVGKQGEVVNVKRGFARNYLVPKKLAIYATPANL
                     KNLANIQSKLADEEQKLMAELKNLAEKIDTTNLVFKRKVDEQGSMFGSVSELDIINGL
                     QELGIQINKSALSMEKHYKELGEFNLPVRLHKDIICSLNIKIEPEQ"
     gene            complement(6336..7172)
                     /locus_tag="PLB65_04135"
     CDS             complement(6336..7172)
                     /locus_tag="PLB65_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77749.1"
                     /translation="MQIFPLIFGAISLLSPFVALIVYMMYIGKTAEVHLTKPLQAFLV
                     FIPVPVLILLFDPQTSSLLLGLDAIFAVGLPVLIFLLTLKHNHILSNAFLAAYLVIAA
                     WGILRYYLFQTYQNQLFEQGLQMVKDRLPALMDKSLTDQTLPIWKAIIPAIWVVSQSI
                     AWLIGFLFFEKNLQIPNKITNLRFPVYYNILIMAVLPLYFFDQTKVLFYNLLITLCVI
                     PFIQGASLVWQRLSLIFTNRFISGLFMIIIVLYANILLVLIGFANMWFSKRNLTIEGD
                     AA"
     gene            complement(7173..7403)
                     /gene="rpsR"
                     /locus_tag="PLB65_04140"
     CDS             complement(7173..7403)
                     /gene="rpsR"
                     /locus_tag="PLB65_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012096455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOQ77750.1"
                     /translation="MNDRKKKYSRKKYCKFCANKDIQIDYKNIDMLKQYISEVGKIEP
                     ARLTGTCAKHQRQLAREIKRARQMALIPYVIE"
     gene            complement(7418..7870)
                     /locus_tag="PLB65_04145"
     CDS             complement(7418..7870)
                     /locus_tag="PLB65_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOQ77751.1"
                     /translation="MADLKLPRLNKCIFSGRIGNDIELKYTPKGTPVVRFVVACDRRY
                     KDDSEQWQTVTSWIECVAWNKWAEFIANQVHKGSPVIVEGRIETRNWTDQNNQNRKTT
                     EVIVEVIHNLEWKPREGEASAVSEDVPLPAEETAPHNATNTTTDDVPF"
     gene            complement(7881..8168)
                     /gene="rpsF"
                     /locus_tag="PLB65_04150"
     CDS             complement(7881..8168)
                     /gene="rpsF"
                     /locus_tag="PLB65_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HOQ77752.1"
                     /translation="MIKDYELMLILNPNQSADEANAMNDSLVALVTENGGEITKIDPW
                     GKRMLAYPINKFNEGYYYVDYFKLDSLLVKKLSASFKINENIIRHQFVVKN"
     gene            complement(8168..8764)
                     /gene="pth"
                     /locus_tag="PLB65_04155"
     CDS             complement(8168..8764)
                     /gene="pth"
                     /locus_tag="PLB65_04155"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOQ77753.1"
                     /translation="MKLIIGLGNPTREFEHNRHNLGFICLDRWAADKGRKFAKGEIFD
                     YIFMHRAVLIKPRTYMNSSGEAVQKAMACWKITDSMVIYDDIELPTAQIRIRQGGGDG
                     GHNGIKSLLTVIPPEELKRIRIGIGRNKEVEPRDYVLSDILPEEWDLLNPTLDVVGKF
                     IDMYIKYDFNAVLNEYSIWKKSYSGADSSGIISPKEES"
     gene            complement(8774..9391)
                     /locus_tag="PLB65_04160"
     CDS             complement(8774..9391)
                     /locus_tag="PLB65_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HOQ77754.1"
                     /translation="MIFNLNAVPRNTVKKSDLNTLRASGMIPAVIYGPKMDSIDISIP
                     KGDFTQMLKKSFTEVSFWDINLNGKQFHTILKDKQVHPVSRDILHIDFMVIAAEALIE
                     IEVPISFIGEALGAKEGGMVEIVQRSVKVSCKANEMPEDLTLDITNLKIGDSLHIRHL
                     PKGNWQFKEPDDVTLVTVHSLKVETPVEPTAAVTPEEHTTPEPEK"
     gene            complement(9406..10353)
                     /locus_tag="PLB65_04165"
     CDS             complement(9406..10353)
                     /locus_tag="PLB65_04165"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOQ77755.1"
                     /translation="MFSKLKLITGNANRPLAEEVARHAGIPLVDIDLFKFSNDESFVK
                     INDNVRGADVFVIQPTCYPVNDNLIDLLIIIDALKRASAQRINCVIPYYAYARSDKKD
                     QPRVPITAKLIADLITVAGAHRVITVDLHADQIQGFFNIPVDHLYAIPTFARYFKSIM
                     PMDDIVVVSPDSGGANRARALAKRLNCTLAIGDKRRSGNEDKTELLNIIGDVKGKSAI
                     LFDDIIDTGGSLIKVSKALVEKGAKKIYAACTHGVLSGNAVVNLEASPIEKLFITNTI
                     PLSEEKKNCQKIIQLSIAEMLAIAIKKVHIEESISILFR"
     gene            10884..12995
                     /gene="fusA"
                     /locus_tag="PLB65_04170"
     CDS             10884..12995
                     /gene="fusA"
                     /locus_tag="PLB65_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOQ77756.1"
                     /translation="MNSDSDSPLSKIRNIGIMAHIDAGKTTTTERILFYSGYLHKMGE
                     VHNGNTFTDWMEQERERGITISSATVTCFWNEYKINIIDTPGHVDFTAEVERSLRILD
                     GAIGVFCGVGGVEPQSETVWHQADRYHVPRIAYVNKMDRIGADYYHVIQMIRERLTPY
                     AFPVNIPIGSEDEFEGVIDLITMQAYYFDPLTCGYEVKHSPVPETYLEQAKKMREELL
                     EKVSEFSDELMMLFLEGKDIPVDLLKSAIRRGTISNKLIPVFCGSSLKNKGIQLLLDG
                     ICDFLPNPMEIEDTQGFDPVTRNPITIASDPNGPLVALAFKVQIDKYVGRLVYIRVYS
                     GTLKKGASFIDQNNSKKERAARILQMMSNRKNDLDNLHAGDIGAIVGSKFLVTGDTIT
                     DGNLDILLSKMHFPDTVISIAIEPKTKSDQENLDIALHRLEEEDPTFRMHIDKDTGQT
                     LISGMGELHLEIIIDRLRREFGIAINQGNPQVAYKETITKEVEAAEVFQREMNGKGNY
                     ASVKFRLSPIALKDLPEDAKNVFINKVNEAQIPSEYWKPIEEAVFNALNDGPLISGNV
                     ERVRVELIGGDFNPVDSNELAYRIATGMAISTGLKGATPVLMEPIMLLTVISPDEFVG
                     DILNDINSKRGKIEVMRRDNEYRQEIVADVPLSELFGYATRIRSLSQGRAIYTLEFKK
                     YDICPVQVQNTVLKKIRGYVE"
     gene            13046..13354
                     /locus_tag="PLB65_04175"
     CDS             13046..13354
                     /locus_tag="PLB65_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOQ77757.1"
                     /translation="MNIKLSIEDKIAKMKIEGILNSENAYLLQEKLNDVLKSDATLLE
                     LDLLDCRNISSTGIGKILLFYKDFITKGGEIEVVRCSNSIYELFSMLKLNQLFTVNLG
                     "
     gene            13357..14274
                     /locus_tag="PLB65_04180"
     CDS             13357..14274
                     /locus_tag="PLB65_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ77758.1"
                     /translation="MDTARILLVDDNISIIENLEMVLQANYFNVDTAYNGRDALHKIE
                     SDEYDLVICDIEMPGISGLDFLSTVRKNYNMELDVILMGSFLEYKDFSETIRLGAADF
                     IRKPIDNNQMLRSINTILKRKNNRNNFSELYNNLERVEFDFVLDPRNFSKFAVSELFN
                     NFLQQNFNLSHNLLNQILICVDEMVYNAFIHGTLALSMQERNLDHHKLQKIINKRLEQ
                     PDIASKRMHIHFSICHKTDTITVTVEDQGPGFDYEFWINKVKEDQKVNLEESGRGISM
                     LYYLSDKLEFQHGGRTVSISKKIPKTNKR"
     gene            14271..15101
                     /gene="prmC"
                     /locus_tag="PLB65_04185"
     CDS             14271..15101
                     /gene="prmC"
                     /locus_tag="PLB65_04185"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOQ77759.1"
                     /translation="MILAELIARAKNAAVANNIPQTDYWLVISYFLKLSRTEIFVFSQ
                     RVLTEQENITLTDAFRRLENGEPPQYITGEAYFYGLELRVNSFTLIPRPETERLVELT
                     LERLQGNEKILDIGTGSGAIAIALKQYLPKVEVYATDCSSSALEVAKANAEKYDVNIH
                     FFLTDCFPPCSEIYDVIISNPPYISKTDLATLDSRIRDKEPKLALYGGEDGLDFYRKL
                     LLQSYAYLTENGFLALEHGDNQKEAITNIAKKEGWTKILSRQDLTGRERYLFISGKKD
                     "
     gene            15104..16360
                     /gene="murA"
                     /locus_tag="PLB65_04190"
     CDS             15104..16360
                     /gene="murA"
                     /locus_tag="PLB65_04190"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOQ77760.1"
                     /translation="MERFIIQGNRNLAGTIKVSGAKNAILPAMAACLLTSGKSVLHNI
                     PNLIDLKTMSHLLRVIGARVDNENGSMTIDARDVCYPEAPYELVSKMRASIYVLGPLL
                     TRLKEVRVSFPGGCAIGTRPVDLHLKAMESLGATVSVEHGYINAKTDGLKGADIYFTK
                     SSVGATINTLMAAVLAEGKTRLFNAAMEPEVDSTIELLNKMGAKIDGIGTTTLEIEGV
                     EELLPAEMDMIPDRIEAGTFLIAGALSKNPVTVANCEPSHLTAVLEKLKEAGCELEIE
                     ESTITVIPPVSIKPVNLMTLPYPGFPTDLQAQFTVLMTLAEGVSFIEDTIFPERYMHI
                     AELNRLQANIKIDRNIAAVRGVKELSGAEVMATDLRASAALVLAGMVANGTTIVSRIY
                     HIDRGYECIEKKLNSIGANIIREEVS"
     gene            complement(16601..17503)
                     /locus_tag="PLB65_04195"
     CDS             complement(16601..17503)
                     /locus_tag="PLB65_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOQ77761.1"
                     /translation="MNPFVEKIIPLILAFFIGIAFKFMKLLSQEDAPVLLKVVLNVCL
                     PCLTINSISNIKLQPNMALIPLTAIIVVLLMYIISTGIGKMLKMEKKTFGSFLVGTMI
                     MNTAYALPFFFAAYGDEGLARASLFDIGNTFMIFTFTYYNAIKYGDNNQTTKIQWKKF
                     LRLPPLWALVIAFAWKLFKAPMPNLATNFLNLMGQPTVPLMMIALGLYFDPKLCNLGK
                     AAIAVFIRMGVGLGIGIALSALFGLSGITKSVVIVCGALPIGFNTLIFANLENMDREF
                     AATIVSISIFISLFYIPWLIYIFR"
     gene            complement(17500..18981)
                     /locus_tag="PLB65_04200"
     CDS             complement(17500..18981)
                     /locus_tag="PLB65_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOQ77762.1"
                     /translation="MIFIYLLSGLFLGWSLGANDTGNIFGAAVETRMLKFKTAALIAS
                     IFIFLGAVLEGGGPSGTLGRLGSVDALGGAFTVALAAALTITVIVRTGIPVSTSQTIV
                     GAIIGWNFFSGRLTDFRNLLTIVASWVIAFVLSGIIAAILFYLIKPIINKGKIHLLEQ
                     DVYIRLGLIIIGAFGAFSLGANNIANVVGVFVPVTPFKDINFGSVTLTGVQQLYILGA
                     ISIIVGIYTYSHKVMRTVGKDIFHLSPVTALIAVTAEAIVLFIFASRSLQKILLSIGI
                     PPIPLVPVSSTQVIVGAVVGIGLVKGGKNIRYNILGKVSLAWIAAPVIAFFFSFITLF
                     IVQNVFEQTVYQKTVYMFNKTVINKIETQGLDTNHLSTVNGRKFDREIEVYKELKQAE
                     CFSRNQILSIIQIAEIYPLKVNTHLLQERGLANHFSRQQWEALVKLEGYEFKHKWQLE
                     EKLSKDFFWQKREEVTERDKLYNKKLDEQMNLLYHTFYLPPSQ"
     gene            complement(18982..19626)
                     /locus_tag="PLB65_04205"
     CDS             complement(18982..19626)
                     /locus_tag="PLB65_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HOQ77763.1"
                     /translation="MALILKTTRFIETQIDAFLDIVSDSATTFELAIEDYLNHRIDQF
                     ENRLSQIRENEHRADDLRVSAERFLYERTLIPENRGDVLAILENTDEVIDHIKDSLVQ
                     FSIEMPQIPEVLNDLWIQTTKASTNAVEQLSFAVRSFFRDLAAVNNYIHKVYFFEREA
                     DHIGERLRREIFNLDIELSHKSQLRFFAIQIEQISDYAQAVCDRLSIYVIKRQL"
     gene            complement(20047..21117)
                     /locus_tag="PLB65_04210"
     CDS             complement(20047..21117)
                     /locus_tag="PLB65_04210"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOQ77764.1"
                     /translation="MRYFRNDLVDKKPSKINVPIKKRMMCLNESSLNPYSAIRDHFLA
                     KMEEVQLNRYLSSITNDLHLALTSYIDNSLCKENVLIGNGADDILYHIFLAVRENNNS
                     YAVSLAPSYFDYKTFSAMVGLKMQFVDLNEDFSFNTDKYLELANHPDCKLAILCNPNN
                     PTGNLFPDEQLWQIITALPNKLILVDETYYEFSGKTFVGDLSAHPNLILVRSFSKAFA
                     AAGLRFGYAISSEENIYELSKVLTTFHTSILNQTFALSILENKDIFQKQVQQTVFLRD
                     ELYNTLKTIEEINVHPSATNFLTFTLSDRTPDFFHYLLANEIAVRDVGSHPVLHNCLR
                     VTISNREDVEAFVQCVKQFINK"
     gene            complement(21195..23087)
                     /locus_tag="PLB65_04215"
     CDS             complement(21195..23087)
                     /locus_tag="PLB65_04215"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOQ77765.1"
                     /translation="MKTNTQDIINRINLQAQKARKAILTMTTLAGSGHPGGSMSSIDL
                     LLCVYNTIKHNPAQPKLPDRDRVVVSNGHISPAVFSTLGIMGYFDLDDAIAEFRLTGS
                     MFEGHIERNVPGVEWSSGNLGQGLSAGTGFALASRINKTPYNVYVFMGDGEQQKGQIS
                     EARRFATKYNLDNIIAIIDYNQLQISGSIHNVMPQNIRKNWEADGWKVIEVNGHDVAL
                     VLDALEKARSFQQPTMILAHTVMGKGVSFMQNNAKYHGSTLDETQLNNALKELGEENE
                     LKRYRKLRMDFKPEKNIPMRFTFTPDIKTGNPIRYETDSDCRSAWGKAIADLVKNNAA
                     AKTPIVVLDCDLATSVKTSEFAEEKPENFFQCGIMEQHTCVMGGALSICGIQTFWSDF
                     GVFGIDEVYNNQRLNDINQTNLKTVVTHVGIDVGADGKTHQCIDYISLARNLFGYKMI
                     FPADANQTDRIIRWLINKPGNYLVTMGRSKIPVLKKIDGSPQYDLDYNYIYGKADVLR
                     DGNDGSMWVCGTPVAKAIKAIDILREEGIFLELLYVSSPLHLDIAQLKAAANKGIIFS
                     VEDHSVNGGLGTSIADGLVANSLSAKLIKMGIKQYPMSGESNELYHWAGLDTASLIAT
                     VKKNLQ"
BASE COUNT         7573 a         4277 c         4049 g         7320 t
ORIGIN      
        1 acccccaacc tattgattac aaatcaattg ctctaccttt gagctacatc ggcaataagt
       61 tttgccacta aattttcagt cctcgatttt gtcaactgct tttttgctta tttcttaact
      121 aatctaaata cgaggattat gaaacactat acttataaat cattatcaca ctatattcac
      181 tgggagcaaa aaaattttaa cccttatctc tgtcaatcct tttcgctctt ttttcaaaac
      241 tgataaaata attagctgat aattatggag cttataacat agcagatatt caagaatccg
      301 tgtagtcttt ttgtttttta aaagccctat agcgcttatc tctcaaattt cccatttgtt
      361 gatagtgata taaaaaagac agagttcagt agctcatagg gatttttcaa ttaagatagt
      421 ttaataattc ttataaattg gggtaaagag ttttgtatca aaaaaggtag aaaaatcaat
      481 tacctcaacg ccagcaattt ttcgtagttt aaagaaatct ggttcacttt gttccaaata
      541 tacctttcgt aacagttccg agcaatataa ttcagaagag tcatttaggt taaaatcgtt
      601 atcaaaagga gttttctgtt tcagaaggac gaggcatctc tttttcatag cagctgaatt
      661 cagttcttta tgatagcgga tgatacagat acgattttgt tttgcttcgt tgataaaatc
      721 ttctagagta ttcatccgga taccatctat tcctgagatg gatttggaaa ttgtatgaat
      781 tacttgatat tgatcctgaa tcttaaaaat aaatccgcaa tgagacatac cttcggctgc
      841 aggaaaattt ctggatataa gttcggataa tatactgttt ccttttctga ggattacatc
      901 tccatccttc aattccagag aggaaagctg ggaaaattca aaagcacttt ccacaaataa
      961 attggcagtt cgaatcgaac ttattgccga actgccagtt aataaaatta gggaaaaggt
     1021 tagaccccgt gaaaaaaggt ctattaaaga cattacttac ctttaatatc taccaaccat
     1081 ttaccgtctt tctttaccat attcatatca aattcgtctt tatttccttc ttcggtagta
     1141 taggaaatat tcactatagc actgtctcca gaaattttag tttcgccaac ggtagcatct
     1201 ttaactttga acatagtaaa cattcccagc atatctttgc tctctggagt ggaaagtttt
     1261 tccatagaat tcatatcttg attctcccaa gcatcgatga acttttgagc aacactttcg
     1321 ggaccctttg cacagcccac aagaacgaaa attagggcaa gagcgctaat ggcaattaat
     1381 aattttttca tcataactcc tttacattgt tattgttaat ttcctatatt cctaaataga
     1441 aaaaaatgtc aagcgttttc aaagaaagta actgcaatat tgaacactaa aacaagcttg
     1501 gcacaatagc aatagtaaaa caaaatgcaa taatgaaaac tgatataaat tgttgatagc
     1561 ttcaatgatg ccaataaaca aaacgagaag taatagataa attagaaagt atagccagta
     1621 aaaagcaaat gtaacttcca tatttaacgt aaagataaaa catccctacc taaaaaaaag
     1681 gaataacctg gaggcacttt tttatccaaa tctcaaagga aaaactccat tcttactcaa
     1741 ttagttgcag tgcaactgta gtggtgctcc ttgggaattt gccgagaggc actaccgtat
     1801 ctacaccata actcattaag caacattcaa tcctgtttct aaaatttgta gagtgtgata
     1861 agttattata acataataca attgagtatt taataagcta aaacaccgag ttatatagtg
     1921 cattaatgat aaaataattg gctttttctc aaaactgtat ataatattaa gtatgaatat
     1981 aatatacagt ttaaggagcg aaaggatgac ccagtattta tgtaaaacct ttttagtgat
     2041 atcgttaact ataatgctat ttttttcagc gtgtatctgt gtgagaaaag ataacattga
     2101 acaaggtgtg aaggttaatg gagttacttt gaactatgaa aatgaagtaa ctctttctga
     2161 aggatatgat tctaattttc taacaataga tggagaaact ctttctttga atattaaggg
     2221 aagctctgaa ccgaaaatta acttacagct aaaatattgg gaatataaac ctggtgatgc
     2281 aaaatttagc ttgaagaaag aaaaattaag ctacgaaact aaatctggaa agcctgtatc
     2341 tttgttcagt ataactggaa gtatacctaa aaacctgaat ctcaatattt ctacgggaac
     2401 aggaaaagta aatttgtcag atatgatagg agagcaaact attacaattc aagctggaac
     2461 cggaagtata atagtttccg aaagcaagct taaaaagctc tctgcagaaa caggaacagg
     2521 atcaatcata atcgataaat gcaatattga tactactttg ctggaatctg gaacaggttc
     2581 tgttcacatg aaaaaaagca acatagatat ggcttcagtg aatacgggga ctggaaacat
     2641 tatcctggaa aatagtaata tcaataagca atattttgaa acaggcacag gtaaggtgat
     2701 tataaacaaa tgaacaaaat atgaaatgat gatagggaga tttggtatat agggaagcga
     2761 taagttatat tagttgagat tcattcttca aatccctata gtctatataa tcctgttctt
     2821 ctcacagcta attttttatc gccacgaagc tctctggtga aggaattttt ttcattccag
     2881 gcttatttaa tttgtgtaat ccttgtgatc ctgtcctctt tttggtaatt actgtaattt
     2941 tccgcccatc attctttcca aatgtttcat tcccttcggt gtgctcactt ttttcatcat
     3001 ttttttcatt tcatcaaatt gttttaagag tcgattaact tctgtaacgc ttcgtccgca
     3061 accttttgct attctcaatc ttctgctacc attaataatt tctggatgtt cgcgttcttt
     3121 ttctgtcatc gattggataa ttgcctcaat atgtttcagt gctttctcat cgatattcag
     3181 atcagcgggg agtttatgtc ccaaacctgg gatcattctg ataatggatt ccagcgaacc
     3241 cattttttta atgctctgca attgtttcag gaagtcgttt aaggtaaatt ggtttttcag
     3301 cattttgcgg gcaagttttt cttcctcttc catatctata gattcttccg ctttttcgat
     3361 gagggttaaa acatcaccca ttcctaaaat acggcttgcc atacggtcgg ggtaaaaaat
     3421 ttccagatcg cttatttttt ctccgatgcc aacaaaagca acaggtttac ctgtaacagc
     3481 tttaattgat aaagctgctc ctccgcgtgc gtcgccatcc atttttgtta aaatcacagc
     3541 atcaaaagct agtttatcat aaaattcttt ggcaatatta acagcatcct gtcctgtagt
     3601 tgcatctgca acaaaaaaga tataatctgg ttttatatga gctttcaaac gcgcaacttc
     3661 ttccatcatc ggttcatcga tatgtaaacg tcctgccgta tcaaaaataa gaagatttac
     3721 catttcttta tcagctatag ataaagcgga atctgcaatg ctcaggacat ttgttgtatc
     3781 ttcagaaatt acaggaacat ttatttgctt acccaaaatt ttaagctgat caatagctgc
     3841 aggacgataa acatcacaag caaccatcat tagtttagta tcttttttac gataaaaaga
     3901 ggccaatttg gcacaagcag tagtttttcc agaaccctgt aaacctacca tcatcacttt
     3961 tgttaggcgg ttgttataaa cattaaactg aaaaccttca gtatccataa gattaacaag
     4021 ctgttcataa acaattttca caacctgttg acccggagta agcgatttca taacttctgc
     4081 acctaaggca cgtttttcca cttcactaat gaagtttttt acaattcgga agtttacatc
     4141 tgcttccaga agtgccatac ggacttctcg tagtccggct ttaatgttat cttcggttaa
     4201 atatcctttt ccgcgtaagt tacgaaatat tttatcaagg cgttcagtta agctatcaaa
     4261 cattataaat cctttgtctt ttatgcattt ttcagagcag ctattgcctg ttcatagtta
     4321 tcgctgctaa aaatatagga agctgaaact aaaatatctg ttcctgcttc ttttatggct
     4381 ttggcattag cggcagtaat tccgccatca acttcaatca agtatcggta gttgttcttt
     4441 ttccgtaaat taaataaatc ccatatttta taaattgctg atggaataaa ttcctgtgct
     4501 gaataaccgg gatttacact catcaccaat atataatcca gatcaggtaa aagggcttct
     4561 aaggttccta atggagttcc tggatttaaa gctaacccag ctttgatacc cctatctttc
     4621 agtttctgaa taaaccgatg actatgataa caggtttcct gatgaaagct gacccattgc
     4681 acattcatat ccagtaagat aggaatatga aaatccggat tagtaaccat tagatggata
     4741 tccaagggaa gcgaggtcat cctacggatt gctttaataa caggaaatcc aaaactgaga
     4801 ttgggaacat aatgtccatc cattatatcc aaatgcagga tatctgctcc tgcagtttcc
     4861 aaggcagcaa tttcggaagc aaggcgtcca aaatctgctg ataatataga aggtgctatt
     4921 tgcgtctttt gcatttacct ttcggactgc taataagaaa gacaatatca gcaatctctt
     4981 cctcatagat atttttatat ttagatatga tgtcatcctt aatcagaatc agttcttgca
     5041 tccaggtatt attgtgtacg gttacaaaaa gaaccttatt ttcaatcttt ataggatacg
     5101 attttgaggc taaaagttcg cccacaactt tcttccagga aaggtaaatg tggatgaaat
     5161 ttttatactt atcgccacct aattgataaa caagctgtcc tgaaagtttt gagatagaac
     5221 tgaaactcat agcaaaataa agctcttcac cggttgccag gcattccggt gaagagtaaa
     5281 atgaatgttt gagctcgttt attcccgatt ggtgattaat ccaaaaccac tataggctaa
     5341 tattagaacg atcagagcaa cataatatac agagccaatg ccaatagttg ttttttcaat
     5401 tctactcatc aataaacctg gaatcatagc cgctatcaca tagaggatat aaccgataac
     5461 tctgttactt acttgtttgc cacgcatatt aataaaatat acagcagcca gagttaaagc
     5521 tataaaaact atagaaacgg ctccgacgat tgcaatagct gtgcctatgg gtaaggattc
     5581 aggtaaattt actaagtttg tcttgaaaat aattaccatt ggagtaatta aagagagcag
     5641 caccagtatt attccaaaga cctttttaaa aacaggggat gattctttaa ctttctcaaa
     5701 atggcgttgt cttttattgg tgaggtaaaa cataattgcc accaccacaa gaaaaaatac
     5761 accataagca aggatcatta tgggtcggcc actgatcatt cctagtaagg atagaactgc
     5821 gacaaatacg atcaaactta atatttgttt gatcttatat cctagatcgg acatcaaaac
     5881 ctccacaatt tattattgtt ccggctcaat tttaatattt aaagagcaaa ttatatcctt
     5941 atgcaaacgc accggcaaat taaactcgcc caattcttta taatgctttt ccatagacaa
     6001 agcactttta ttaatctgaa ttcccagctc ctgtaaacca ttgataatat ctagttcaga
     6061 aacggaacca aacatactac cttgttcatc aaccttgcgc ttaaaaacca aattagtagt
     6121 atctattttt tcggcaaggt tttttaattc agccatcaat ttctgctctt catccgctaa
     6181 tttactttgg atgttagcaa gattttttag attggctgga gttgcgtata tagctaactt
     6241 tttcggcact aaataatttc tggcaaagcc gcgttttaca tttacaactt caccttgttt
     6301 tccaactttt tctatcgttt tcagcataat aactttcatg ctgcatctcc ttcaatagtt
     6361 aaatttcttt tactaaacca catatttgca aatcctataa ggacaagcaa aatattggca
     6421 tagagaacaa tgattatcat aaaaagacca ctgataaacc ggttggtaaa tattaaactc
     6481 aaacgctgcc aaactaaaga cgctccctga atgaaaggaa tcacacataa agtaatgagc
     6541 aaattgtaaa acaaaacctt tgtctgatca aagaaataaa gaggtaacac ggccataatc
     6601 agtatgttat aataaaccgg aaacctcaaa ttggttattt tgttcggtat ctgcaaattt
     6661 ttttcaaaaa agagaaatcc aatcaaccaa gctatggatt gtgaaacaac ccaaatcgct
     6721 ggaattattg ctttccaaat tggcagggtt tgatccgtta aagatttatc catcaaagcc
     6781 ggcaatctat ctttcaccat ttgcaagccc tgttcgaaga gctgattttg atatgtttgg
     6841 aaaagataat acctcaatat tccccaggca gctataacta aataagcagc caaaaacgca
     6901 ttactgagaa tatgattatg ctttaaagtg agcaaaaaga tcaaaaccgg caatcctaca
     6961 gcaaaaatgg catccaaacc aagcaaaaga gaactagttt gaggatcgaa taataatatc
     7021 aataccggaa ccggaataaa cactaaaaag gcttgtaacg gtttagttaa atgcacttca
     7081 gccgtcttgc caatatacat catatacact attaaagcta caaaagggct taatagtgat
     7141 atagctccaa atataagagg aaagatttgc atctactcaa ttacataagg aattaaagcc
     7201 atttgtctgg cacgctttat ttctctggca agctgacgtt gatgctttgc acaggttcca
     7261 gttaaacgag ccggttcaat ttttcccact tcggaaatat actgtttcag catatctata
     7321 tttttataat cgatctgaat atctttatta gcgcagaatt tacagtattt cttccgagag
     7381 tattttttct ttctatcgtt catttttttc ctctctctta aaagggaaca tcgtcagttg
     7441 tagtattagt agcattatga ggggctgttt cttctgcagg taaaggaaca tcttcagaaa
     7501 ctgctgaggc ctctccttca cgaggtttcc attcaagatt atgaataact tctactataa
     7561 cttcggttgt tttacggttt tgattatttt gatctgtcca attgcgggtt tcgattctgc
     7621 cttcaacaat cacagggctg cccttatgaa cttgattggc aataaattca gcccatttat
     7681 tccaagctac gcattcgatc caactggtaa ctgtttgcca ttgttcgcta tcatctttat
     7741 aacgacgatc acaagcgaca acaaaacgta ctacgggagt tccttttggc gtatatttca
     7801 gttcgatatc gttaccgatt cttccactga aaatacattt gtttaaacgc ggaagtttta
     7861 aatctgccat tatattctcc tcaatttttc accacaaatt ggtgacgaat gatgttttca
     7921 ttgattttga acgaagcgct tagtttttta actaataagc tgtccagctt aaaataatcc
     7981 acatagtaat aaccctcgtt aaatttgttt atcggataag ctagcatacg ctttccccaa
     8041 gggtcaattt tggtgatctc tcctccattt tctgtaacta aagccactaa actatcattc
     8101 atagcattgg cttcatctgc actttgatta ggatttaaga tcagcattag ttcgtaatcc
     8161 tttatcatta actttcctcc tttggactaa tgattccaga actgtctgct ccggaatagg
     8221 attttttcca gatactatat tcattcaaca ctgcgttgaa atcatattta atatacatat
     8281 caataaattt gccaacaaca tcaagtgttg gattcagcaa atcccattct tcaggtaaaa
     8341 tatcacttaa cacataatct cgcggttcaa cttctttgtt tctgccaatt ccaatccgaa
     8401 ttctctttaa ttcttctggg gggataactg tcagcagaga tttgatgcca ttatggcctc
     8461 catctcctcc cccttgacga atgcggattt gagctgttgg taattctata tcatcataaa
     8521 taaccataga atctgttatt ttccaacatg ccattgcctt ttgaactgct tccccggaac
     8581 tattcatata ggttcttggc ttaatcaaaa cagctctatg cataaaaata taatcgaaga
     8641 tctcgccttt ggcaaatttt cttcccttat cggctgccca acggtctaaa cagatgaaac
     8701 ccaaattgtg acggttatgc tcaaattctc ttgtaggatt tcccaatcca atgatcagtt
     8761 tcattactat agattatttt tctggttcag gagttgtatg ctcctcaggg gttacagcag
     8821 ctgttggctc aactggagtt tccactttta aagaatgaac tgtcacaagc gttacatcat
     8881 cgggttcttt gaattgccaa ttgcctttgg gaaggtggcg aatgtgaagt gaatcaccta
     8941 ttttcaaatt agtgatgtct aaagttaaat cctctggcat ttcatttgct ttacaggata
     9001 cttttacaga gcgttgaacg atttctacca ttccgccctc tttcgctcca agtgcttctc
     9061 ctataaaact aataggaact tcaatttcaa ttagggcttc tgctgctata accataaagt
     9121 caatatgcaa aatatctcta ctaacaggat gcacttgttt gtctttcaag atagtatgaa
     9181 attgtttacc atttaggttt atatcccaga agctcacttc agtaaaactc tttttgagca
     9241 tctgagtaaa atcacccttg ggaatgctaa tgtcaatact atccatttta ggcccataga
     9301 ttacagctgg aatcattccg ctggctctca gggtgttcag atcggatttt ttaaccgtat
     9361 ttcggggaac ggcattcagg ttaaatatca ttattcctcc attttctatc taaataagat
     9421 actaatcgat tcttctatat gaactttctt aattgcaatt gccagcattt cagctataga
     9481 aagttgtatt attttctggc aattcttctt ctcttctgaa agaggtatcg tattggtaat
     9541 aaatagtttc tctattggag atgcttcaag attaactact gcatttccgc ttaaaacacc
     9601 atgcgtgcaa gcagcataaa tctttttagc tcctttttct actaacgctt tagatacttt
     9661 tattaagctt ccacccgtat ctataatgtc atcaaataat atagctgatt tgccttttac
     9721 atcaccaata atattcagga gttctgtttt atcctcattc ccacttctgc gtttatcacc
     9781 aatagctaat gtacaattta aacgttttgc taaagctctt gctctatttg ctccacccga
     9841 atcgggagaa actacaacta tatcatccat cggcataatg cttttaaaat agcgggcaaa
     9901 agtaggaata gcataaagat gatctacagg aatattgaag aaaccttgaa tctggtctgc
     9961 atgtaaatct acagtaatta ctctatgcgc acctgccaca gtaatcaaat cagcaatcaa
    10021 ctttgctgta attggaactc ggggttggtc ttttttatca gaacgagcat acgcatagta
    10081 aggtatcaca caattaattc gctgtgctga agctcttttt aaagcatcaa taatgatcag
    10141 tagatctatt aaattatcgt taaccggata acaagtcggt tgaattacaa aaacatcagc
    10201 accgcggaca ttatcgttta ttttaacaaa cgattcgtca ttagaaaatt tgaacaaatc
    10261 tatatctacc agaggaatac ctgcatgacg agctacttcc tctgctaaag gacgattagc
    10321 atttcctgtg ataagcttta gttttgaaaa cacttgcttc ccttcttttt tgttttccct
    10381 gatcagttaa ttttcctctt tctgcattgc cttttcagct atgcaagtta ctaaatatct
    10441 atcttttata tatctgttcc cagataaaat aaactatgtt aatccacaat tttatagcta
    10501 tgctttttgt caaagcttat ttctttttag cacgcatacc gctaatattt tgaataatca
    10561 atgtactata tatcaatttt agggcaatca atagaaatca ccttacctga ttgattataa
    10621 tactatttct ttatggaaga gctatttatc attctggctt taaaagaacc agataatttt
    10681 tataataacc gcatcctcgt tatgcaaact atatcagtta tttcagcagg gtccattgct
    10741 ttcctaatgc tcatatatat tgatgctagt acagttaatt tcttcaatta tttgcatttt
    10801 agatagccac aagctacttt tttcatagac aaacaaacta accttgtttt aatggttcac
    10861 aaaacatgaa aaaggaattt tgtatgaaca gcgattcgga tagtccacta tcaaaaatac
    10921 gcaatatagg cattatggct catattgatg ccggaaaaac gacaaccacg gaaaggatat
    10981 tgttttatag tggttatttg cataaaatgg gagaagtgca taatggcaat acctttacgg
    11041 attggatgga acaggaacgt gaaagaggaa taactatatc atcagctact gttacttgtt
    11101 tctggaatga atataaaata aatattatag acactcctgg tcatgtagat tttacagcag
    11161 aagtagaacg ttcccttcgt attttagatg gagcaatagg agttttttgt ggagttggag
    11221 gtgtggaacc tcaatccgaa acagtttggc atcaagcaga tcgatatcat gttccccgga
    11281 ttgcttatgt gaataaaatg gaccggattg gagcagatta ttatcatgtt attcaaatga
    11341 ttagagagcg gcttacgcct tatgcttttc cggtaaatat tcctataggc agcgaagatg
    11401 agtttgaagg tgtaatcgat ttgatcacaa tgcaggcata ttattttgat cctttaacct
    11461 gtggctatga agttaagcat tctcctgttc cagaaactta tttggaacaa gcgaaaaaaa
    11521 tgcgggaaga actattagaa aaagttagtg aattcagtga tgagcttatg atgctatttc
    11581 tggaaggaaa agatatccct gtagatttat taaaaagtgc aattcgccga ggaacaatta
    11641 gcaataaatt gattccggtt ttttgtggaa gttcgcttaa aaataaaggt atacagctgc
    11701 ttttagacgg aatttgtgat tttttgccaa atcctatgga gattgaagat acacaaggtt
    11761 ttgaccccgt aacccgtaat ccgataacaa ttgcttccga tcctaatggt cctctggtag
    11821 ctttggcctt taaggttcag atcgataaat atgttggtcg ccttgtttat attcgtgttt
    11881 attccggaac tctaaaaaaa ggtgcttcct tcattgacca gaataatagc aaaaaagaaa
    11941 gagcagcacg cattttacaa atgatgagta atcgcaagaa cgatttggat aatttgcatg
    12001 caggtgatat tggagctatt gtcggttcaa aatttttagt tactggggat acaattaccg
    12061 atggtaacct ggatattttg ctctctaaaa tgcactttcc ggatactgtt atctctatag
    12121 ctattgaacc aaaaaccaaa tcagatcagg aaaatctgga tatagcactg catagattgg
    12181 aagaagaaga ccccactttt aggatgcata tagataaaga tacaggacag acattgattt
    12241 ccggaatggg agaattgcat ctggaaatta taatagatcg attaagacgg gaattcggaa
    12301 tagctataaa tcagggtaac cctcaagttg cctataagga aactattacc aaagaagttg
    12361 aggcagcaga ggtcttccag cgcgaaatga atggaaaagg aaattatgct tctgtgaagt
    12421 ttcgcttaag cccaatcgca ttaaaagact tgcctgaaga tgctaagaat gtctttatta
    12481 ataaagtaaa tgaagcccaa attccctctg aatattggaa gcctatagaa gaagcagttt
    12541 tcaacgcact aaatgatgga cccttaatta gtggaaatgt ggaaagagtt cgcgtggaac
    12601 ttattggtgg ggattttaat ccagtagatt caaatgaact tgcgtatcga attgcaacag
    12661 gtatggctat aagtacaggt ttaaaagggg ccacacctgt tttaatggaa cccattatgt
    12721 tgctgacagt tatctctcct gatgaattcg tgggcgatat tctaaacgat attaattcca
    12781 agcgtggcaa aattgaagtt atgcgtaggg ataatgaata taggcaggaa attgtagcag
    12841 atgttcctct ttcagaactt ttcggatatg ctactcggat tcgttcttta agtcaaggaa
    12901 gagctatata caccctagaa ttcaagaaat atgacatctg tcctgtacaa gttcaaaata
    12961 ctgttttgaa gaaaattcgc gggtatgtgg aataaatgct tgacaaataa aggaaataaa
    13021 aaaatgttaa agtaaggagg aaaagatgaa tattaagcta tccatcgaag acaaaatcgc
    13081 taagatgaaa attgaaggta tcttgaatag cgaaaatgca tatctgcttc aagaaaagct
    13141 aaatgatgtg ctaaagtccg atgctactct attagagctt gatctacttg attgccgcaa
    13201 tataagctct accggaatag ggaaaatttt gttgttttac aaagatttta ttacaaaagg
    13261 aggagaaatt gaagtagtcc gatgctcaaa cagtatttat gagctatttt caatgcttaa
    13321 actgaatcaa ttatttacgg taaatttggg ttgacaatgg atacagctcg cattctatta
    13381 gtggatgata acatttctat aatcgaaaat ttggagatgg tactgcaggc taattacttt
    13441 aatgtagata ccgcttataa tggaagagat gcactacata aaattgaatc ggatgaatat
    13501 gatttggtga tttgtgatat cgagatgcca ggcattagtg gattagattt tttatctaca
    13561 gttcgtaaga attataatat ggagttggat gttatcctga tgggtagttt cctggaatat
    13621 aaagatttta gtgaaactat tcggctcggt gctgctgatt tcattcgtaa acctatagat
    13681 aacaatcaaa tgctgcgttc tatcaataca attttgaagc gcaagaataa tcgtaataat
    13741 tttagcgaat tatataataa cttggaaagg gttgaattcg attttgttct tgatccgcga
    13801 aacttctcca aattcgcagt atcagaatta ttcaataact ttttgcaaca gaatttcaat
    13861 ctctctcata atctgctaaa ccaaatcctg atctgcgtcg atgaaatggt ttacaatgct
    13921 tttattcatg gaactttggc attaagtatg caagagcgca atttggatca ccataagctt
    13981 caaaaaatta ttaataaacg tcttgaacaa cctgatattg cctcaaaacg gatgcatatt
    14041 catttctcca tttgccataa aacagatact atcactgtaa ctgtagaaga tcaaggacct
    14101 ggctttgatt atgaattttg gattaataaa gtaaaggaag accagaaagt aaacctggaa
    14161 gaaagtggca gaggaatatc aatgctttat tacctttctg ataaacttga gtttcaacac
    14221 ggaggcagga cggtttcgat cagtaaaaaa ataccaaaaa ctaataaaag atgatcttag
    14281 cagagctgat cgccagggca aaaaatgctg ctgtagctaa taatattcct caaactgatt
    14341 actggttagt aatatcctat tttttaaaat taagcaggac agaaattttt gttttcagtc
    14401 agcgagtatt aactgaacag gaaaatataa cccttacgga tgcttttagg cgtttggaaa
    14461 acggtgaacc tcctcaatac ataactggag aagcatattt ttacggttta gaattgagag
    14521 taaattcttt tactttaatc cctcgtcccg aaactgaacg cttggtagaa ttaactttag
    14581 agcgtttaca gggaaatgaa aaaatcctgg atattggaac aggaagtgga gcaatagcaa
    14641 ttgctctaaa acaatattta ccaaaggtgg aagtgtatgc tacggactgt agttcatccg
    14701 ctttggaagt ggctaaagct aatgcagaaa aatatgatgt taatattcat ttttttctta
    14761 ccgattgctt tcctccctgc tcagaaatat atgatgttat aatttccaat ccaccatata
    14821 tcagcaaaac agatttggca actttggatt ccagaattag agataaagag ccaaaacttg
    14881 ctctatatgg aggagaagac ggattggatt tttatcgaaa attacttctg caaagttatg
    14941 cctatttaac tgaaaatggt tttctggctt tggaacatgg tgataatcaa aaggaagcta
    15001 tcacaaatat agccaaaaaa gagggctgga caaaaatcct atcccggcaa gatttgaccg
    15061 gaagagaaag atacctgttt atttcaggta aaaaggacta aagatggaac gttttattat
    15121 ccaaggaaat cgtaatttgg ccggtactat taaagtaagc ggtgctaaaa atgcaattct
    15181 accagctatg gctgcttgtt tattaacctc ggggaaatcg gtgttacata atattccgaa
    15241 tttgatagat ctgaaaacga tgtctcattt attgagagtt attggagcca gagttgataa
    15301 cgaaaatggc agtatgacta ttgatgcgcg tgatgtatgt tatccagaag cgccttatga
    15361 acttgttagt aaaatgcgtg cttctatcta tgttctgggt cccctcttaa ctcgtttgaa
    15421 agaagtaaga gtttcttttc caggtggttg tgcaattgga accagacctg ttgatctgca
    15481 cttgaaagca atggaaagct tgggagccac tgtgtctgtt gaacatggat atataaatgc
    15541 caaaaccgat gggttgaagg gtgcagatat ttattttaca aaatcaagcg taggagctac
    15601 tatcaataca ttaatggcag ctgttttagc tgaaggtaaa acaagattat tcaatgccgc
    15661 tatggaacca gaagtggatt ccactatcga gttattgaat aaaatggggg caaaaattga
    15721 cggtatagga acaactactc tcgaaattga aggtgtggaa gaacttttac ctgcagaaat
    15781 ggatatgatc cctgatagaa ttgaagctgg aactttttta atagcagggg cacttagtaa
    15841 aaatccggta actgtagcta attgtgaacc ttcacatctc acagctgttc tggaaaaatt
    15901 gaaagaagca ggttgtgaat tggaaattga agaaagtacc ataacagtta ttccacctgt
    15961 ttctatcaaa ccggtaaatc tgatgaccct tccctatcct ggttttccaa ctgatttgca
    16021 agctcaattt acagttctaa tgaccctggc tgaaggagtt agttttattg aagatactat
    16081 ctttccagaa cgctatatgc acatcgcaga attaaaccgt ctgcaggcaa atatcaaaat
    16141 tgatagaaat attgctgctg tccgaggagt aaaagaactt agcggtgcag aagttatggc
    16201 tacagatcta agagccagtg cggctcttgt tttagcaggt atggttgcta atggaacaac
    16261 tattgtttcc agaatttatc atatagatcg cggctatgag tgtatagaaa aaaagctaaa
    16321 ttcgatcggc gctaatataa ttagagaaga agtatcataa tcgcctactg ctaaaaacat
    16381 caaagtaaaa ttcgaattaa agcagagtaa ctttatcctg aatccgaaaa aagttattcc
    16441 ataattctct aatgattatt tatattagca attagcggta gctttatata tcaatatatc
    16501 aacatttact ggggtagtat agaaatcctt catccagaat attattttcg gaaggtggca
    16561 tcttccttac aaataaatat gtagctccag atacagagaa ttaccggaaa atataaatca
    16621 gccaaggaat atagaaaagg gaaataaaga tagaaatgga gactatggtc gccgcaaatt
    16681 ctctatccat attttccaga ttagcaaaaa tgagggtatt gaaaccaata ggcaatgcac
    16741 cacaaacaat aactacagat tttgttatac cgcttagacc aaatagcgcg cttagggcaa
    16801 ttccaattcc caatcccaca cccattctaa taaagacagc aattgctgct ttacccaggt
    16861 tacaaagttt aggatcaaaa tataaaccca aggcaatcat cattaaagga acagtgggtt
    16921 ggcccataag attgagaaaa ttcgtagcta aatttggcat tggtgctttg aataatttcc
    16981 aggcaaaggc aatcaccaga gcccaaagag gaggaagacg caaaaatttt ttccattgaa
    17041 ttttggtggt ctgattatta tctccgtatt tgatagcatt ataataggta aaagtgaaga
    17101 tcataaaggt attgccaata tcaaacaatg atgctcgggc tagtccttca tcgccataag
    17161 cagcaaagaa aaagggtaaa gcataagcgg tattcataat cattgtgcct accaaaaagc
    17221 taccaaaggt ttttttttcc atcttcagca ttttccctat ccccgtggag ataatataca
    17281 tcagcagcac aacaattatt gctgttaaag gaatcaaagc catattgggt tgtaacttaa
    17341 tgttggaaat ggagtttatc gttaaacaag gcaaacatac atttaaaact actttcaaaa
    17401 gaactggagc atcttcttgg gaaagaagct tcataaattt gaaagcgatg cctataaaga
    17461 aggccagaat aagaggaatt atcttttcaa caaaaggatt cattgcgaag gaggaagata
    17521 aaatgtatga tatagcaagt tcatttgttc atccagcttt ttattataga gcttatctct
    17581 ttcggtaact tcttctcttt tttgccagaa aaaatcttta gacagctttt cttcaagctg
    17641 ccatttatgc ttgaattcat atccctccaa tttaaccagc gcttcccatt gctgtctact
    17701 gaaatgattg gctaaacctc tttcttgtaa tagatgggta tttactttta aaggatatat
    17761 ttcagctatt tggatgatgc tcagaatctg gtttcggcta aaacattccg cttgtttcaa
    17821 ctctttataa acctctattt ctctatcaaa tttgcgtcca tttactgtag aaagatgatt
    17881 ggtatctaat ccctgtgttt ctatcttgtt gatcactgtt ttgttaaaca tatatactgt
    17941 tttctgataa acagtttgtt caaagacatt ttgaacaata aacaaagtaa taaaagaaaa
    18001 gaaaaaagca atcaccggag cagcaatcca agctaatgag accttgccca atatattgta
    18061 tctgatattt ttcccaccct ttactaaacc tatgcccact accgctccta caattacttg
    18121 tgtggaagat actggaacaa gtggaattgg tggtatacca atacttaaca gtattttttg
    18181 cagcgagcgt gaagcaaaaa taaaaagaac aattgcctca gctgttacag ctataagcgc
    18241 agtcacaggt gaaagatgaa aaatgtcctt tcccactgta cgcataactt tatgagaata
    18301 tgtataaata cccacaatta tagaaatggc gcccaaaata taaagttgct gcacccccgt
    18361 taaagtaaca ctgccaaaat tgatatcctt aaaaggagta acaggaacaa aaaccccaac
    18421 tacattagct atattattgg cacccaaaga aaaagcacca aaggcaccaa ttattatcag
    18481 ccccaaacgg atataaacat cctgctccaa aagatgaatc ttacccttgt tgataatagg
    18541 cttaatcaag taaaaaagga ttgcagctat aattccagaa agtacaaaag caattaccca
    18601 actggcaaca atagttagca aattacggaa atctgtaagc cgtccagaaa agaagttcca
    18661 accgatgatc gcacctacaa tagtttgaga tgtggatacc ggaattcctg ttctgacaat
    18721 gacagtaatt gttaaagcag cagctaaagc aacagtaaat gcccctccta aagcatccac
    18781 acttcctaaa cgacctaaag tgccagatgg tcctccccct tctaggacag cacctagaaa
    18841 gataaaaatg gaggcaatta gggcagctgt tttaaatttc aacattctgg tttctacagc
    18901 agcaccaaat atattgccag tatcgttggc tcctaaagac caacctaaaa aaagaccact
    18961 aagcagataa ataaaaatca tctaaagctg tcttttgatt acataaatag atagacgatc
    19021 acatactgct tgggcataat ctgaaatttg ctcaatctga atagcaaaaa aacgtagctg
    19081 gcttttatgg gaaagctcaa tatccagatt aaatatttca cgacgaaggc gttctccgat
    19141 atgatctgct tcccgctcaa agaaatatac cttgtgaata taattattca cggcagctaa
    19201 atccctaaaa aatgaacgaa cggcaaaaga aagttgttca acggcattgg tcgatgcctt
    19261 ggttgtttgt atccataggt catttagcac ttcaggtatt tgaggcattt caatagagaa
    19321 ttgaactaaa gagtctttta tatggtcaat aacttcatcg gtattttcta atatggcaag
    19381 aacatctccc ctgttttcag gaatcaaggt acgttcataa agaaagcgtt cagcactcac
    19441 cctcaaatca tctgcccgat gttcattttc cctaatttgc gataagcggt tttcgaactg
    19501 atcgatccga tgattcagat aatcttcaat tgccagttca aaagtagttg ctgaatcgct
    19561 tacaatatct aaaaaagcat cgatctgggt ctcaatgaaa cgagtcgttt ttaagattag
    19621 cgccattctc tctcccttaa gcatatttta tgttattata tataatttac cagaaagata
    19681 gattgtactt tctgtcaagc aggaaaaaag gtataggaca cagattttgt ggattacaat
    19741 agattaatgc ctgtaacgaa taatagaata ggtctgagat gagcaggatt tttttgcaga
    19801 ggatttaagg taaagagtta attgttaccc gcagataaaa tattctttct caaatttaat
    19861 tggttgcaat gccaaaatat gtaattctgc tcttgatgct taagtccata tagacaataa
    19921 aaaagccatt ttacagtccg gatgaatgcc tgatgtaagc agatataacg ataaagaaca
    19981 taagccatat gcgaatgtta aataagcata ggagacaaaa aatagaaact gcagatgact
    20041 tcttacttac ttatttataa actgttttac acattgcaca aatgcttcaa catcttccct
    20101 attactgatt gttaccctta agcaattatg caaaaccgga tgcgaaccta catcacgaac
    20161 agctatttca ttagccaata gataatggaa aaaatccggt gtcctatcag atagcgtgaa
    20221 agtgagaaaa ttggtagcgg atggatgtac atttatttcc tctatagttt tcaaagtgtt
    20281 ataaagctca tctcgtaaaa aaactgtctg ttgtacctgc ttttgaaaaa tgtccttatt
    20341 ttccagaata gagagagcaa atgtctgatt caagatggaa gtgtgaaaag tggtaagcac
    20401 tttggataac tcgtatatat tttcttctga agatatagca taaccaaagc gaagccctgc
    20461 cgctgcaaat gctttagaaa aagaacgcac aagaattaaa ttgggatgag cggaaagatc
    20521 tcctacaaag gttttcccag aaaattcata ataagtttca tctactaaaa tcagtttatt
    20581 tggtaaagct gtaattattt gccataattg ttcatcagga aataaattgc ccgtaggatt
    20641 attaggattg catagaattg ctaatttaca atcaggatga ttggcaagct ccaaatattt
    20701 gtccgtatta aaactgaaat cctcatttaa gtccacaaat tgcattttca aaccaaccat
    20761 cgcactaaag gtcttataat caaaatagga aggagccaaa gaaacagcat aggaattgtt
    20821 gttttcgcgc actgccaaaa agatatggta aagaatatca tcagcgccat ttccaattag
    20881 gacattttct ttgcataacg aattatcaat gtatgatgtt agcgctaaat gtaaatcatt
    20941 cgttattgag gaaagatagc gattcagctg cacttcttcc attttagcta aaaaatgatc
    21001 tcttattgcc gaatagggat ttagactact ctcattcaag cacatcattc tttttttaat
    21061 gggaacattg attttagagg gttttttgtc cactagatcg ttacgaaaat atcgcatttg
    21121 tttactcctg atattcattt atttattata agctgaaaac agggaaaagt aaagctgaaa
    21181 aaattgtatt cgctttactg tagatttttc tttacggtag ctatcaatga agcagtatcc
    21241 aagcctgccc aatgatataa ttcgttagat tctccggaca tcggatattg cttaataccc
    21301 atttttataa gttttgcgct aagagaatta gctaccaaac catcggcaat agaagttccc
    21361 aaaccaccat taacagaatg atcttccacg ctgaagataa ttcctttgtt ggcagcagct
    21421 tttaattgag ctatatctaa atgtaagggg gaagaaacat ataacaattc tagaaagata
    21481 ccttcttcgc gtaagatatc aattgccttt attgctttgg ccacaggggt tccacaaacc
    21541 cacatactgc cgtcatttcc atctctaagg acatctgctt ttccgtaaat atagttataa
    21601 tccaaatcat attgcggcga tccatcaatt tttttcagca ccggaatttt tgaacggccc
    21661 attgtaacca gatagtttcc tggtttattg atcaaccacc ggataattct atcggtctga
    21721 ttagcatctg ccggaaagat cattttataa ccaaacagat tgcgtgccaa agagatataa
    21781 tcaatgcact gatgtgtttt cccgtctgct ccaacatcta tacctacatg ggtaaccaca
    21841 gttttcagat tagtttggtt aatatcattt aagcgttgat tgttataaac ttcatcaatg
    21901 ccaaaaactc caaaatcact ccagaaggtt tgaataccac aaatggaaag ggctcctccc
    21961 atcacacagg tatgttgttc cataattcca cattggaaaa aattctctgg tttttcttcg
    22021 gcaaattcac tggttttaac actggtggct aaatcacaat ctaaaacaac aattggtgtt
    22081 ttagcagctg cattattttt taccaaatct gctatagctt taccccaagc acttctacaa
    22141 tcgctatctg tttcataacg aatagggttg cctgttttta tatccggggt aaaagtgaag
    22201 cgcataggta tatttttttc aggcttaaaa tccattctca atttacgata tcttttcagt
    22261 tcattctctt cacccagttc tttcaaggca ttatttagct gtgtttcatc caaagtggaa
    22321 ccatgatatt tagcattatt ttgcataaat gaaacacctt tacccataac cgtgtgtgcc
    22381 agaatcatcg tgggctgttg gaaactgcgt gccttttcca gagcatcaag aactaaagca
    22441 acatcgtgac catttacttc aattactttc cagccatcgg cttcccaatt ttttcgtatg
    22501 ttctgaggca taacattatg aatagaaccg cttatctgaa gctgattata gtcgattatt
    22561 gcaattatat tatccagatt atattttgtg gcaaagcgtc ttgcttcact aatctgacct
    22621 ttttgctgtt ctccatcgcc cataaaaaca taaacattat aaggggtctt atttattctg
    22681 gatgcaagag caaagccagt tccggcagat aaaccctggc ccaaattgcc actgctccat
    22741 tcaactcctg gaacatttcg ttctatatga ccttcaaaca tactaccagt taagcgaaat
    22801 tccgctatag catcatccag atcaaaatat cccattattc ccaaagtgct gaaaacagca
    22861 ggagaaatat gcccattgct aactacaact ctatctctat caggcagttt aggttgagca
    22921 ggattatgtt tgatagtgtt atacacacat aaaagaagat ctattgaaga cattgaaccg
    22981 ccaggatgac ctgaaccagc aagagttgtc attgttaata ttgccttacg tgctttttgt
    23041 gcttgaaggt taattctatt aattatatct tgagtattgg ttttcatttg ttattcctta
    23101 tttataatat catttgcaaa aagaattaaa tccatcacca aatatttgtt ggtaaaggaa
    23161 aaaagtggtg gagcatagcg ggatcgaacc gctgacctca tgactgccag tcatgcgct
//
LOCUS       DAOSTH010000020        22860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_2436_length_22860_cov_13.674019, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000020 DAOSTH010000000
VERSION     DAOSTH010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 22860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22860
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2436_length_22860_cov_13.674019"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..627
                     /locus_tag="PLB65_04220"
     CDS             223..627
                     /locus_tag="PLB65_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HOQ77766.1"
                     /translation="MPRSIARISIWKKAVPLLVIILSSFLGIASRKFGYILPPFLAKY
                     TGDTMWALAFCALFRMLFTRQRNWEIAIIAFDFSCLIELSQLWHPVWLDRIRSTVFGG
                     LLFGFGFRYIDLFCYAAGCVLGLFIFSLIPEG"
     gene            755..1630
                     /gene="dapA"
                     /locus_tag="PLB65_04225"
     CDS             755..1630
                     /gene="dapA"
                     /locus_tag="PLB65_04225"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOQ77767.1"
                     /translation="MLQGTYVALITPFKNGSIDWTALENLIQFHLENGTTGILLLGTT
                     AETSALASDEKDALLRFAMKKIDSKLPVMIGTGTNNLHQTLASTQKAKELGADYALII
                     TPYYIKPTQKGMLEYYGIIASKVDIPIIIYNVPGRTSVNMNAATTVELAKTYPNIVGV
                     KDATGNIVQATQIIRDAPDNFSLMSGEDAMNLPLIAIGGKGTISVTANVAPRLVSEHI
                     STCLQGDFVTAAKQHQYLARLNEMMFIETNPIPAKEALAMMGKINLAFRSPMCPLLET
                     NRAMLKQCLQDYNLL"
     gene            1640..4561
                     /locus_tag="PLB65_04230"
     CDS             1640..4561
                     /locus_tag="PLB65_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOQ77768.1"
                     /translation="MKQKELIYGFQLIEKREIKEISTTAHLYKHIKSGAELIYYECDD
                     DNKVFMIGFKTVPEDNTGCPHILEHSVLNGSRNFPAKTTFMELVKGSMNTFINAMTFP
                     DMTCYPVASTNDKDFINLMRVYLDAVLFPNIYEQPNILHQEGWHLELTSEDAPLNYRG
                     VVYNEMKGALSSPENIIGRKSLHAQFPDSPYSFESGGDPDSIPELTYDNFIAFHKKYY
                     HPSNSKITLYGNLNLADTLKIIDAEYLSQFEDSKQKVHLPLQKPFAKAKVTKMDIPLE
                     EGKDIEEQYYLSLNWTYGLITDKYLPQALKALAEMLMDTPASPLKKVIRDSGYAKDSF
                     VYVHNDILQPTISLICKQVKQENIEPLVKLIKQELENIVKNGFDKKLVEAIINKLEFS
                     LREAQYQYYPKGLIYALNSQALWMHTGNPMDYLAFEPMLKELRRGLEEPYFENLIEKV
                     LLYNKHSHQIVFEPVPGLIQKKEQETAAKLAELKKKMSKKEIAKLLEFNQQLIQWQQE
                     PEKKENLEKIPVLSLSDLNPLSKHYPIEEEIKADYTLLKHPLTTNGIVYFKAYFDIAH
                     AEEEDLPWIKLYTQLAEWMNSENYNFAQRSTEIDINTGGITLDIDLYNNYQTPDDILP
                     KIVLAGKAVSTKFGKLMELASEYALKPMFDDPQRIKQLLAELKAKTETMLPFQGHRIA
                     IHRMLKPISHLYHWRDISSGLGYYHFLCDLVNNMDSAIDDIIEELNWIKKTFFTTHNL
                     IISITADEEDILPSTNELANLISCVSGEAFAPIKNLYAIRDYNEGIYAPIQVQYCAKG
                     GNFFRRGYSYSGKLRVLNNILRSNYLHKELRIKGGAYGNMSDFTPGGYVYFVSYRDPN
                     LRETLEVFDSVPEFLRSFDCDKQEFDKYIIGEISALDYPLTPEGTGEKADESYITGFS
                     YEDRQQIRDEVLSAKKEDIRNYADLVEAVMLRNHYAVFGSETKVKEAADIFDAIIPAL
                     K"
     gene            4585..5649
                     /gene="rsgA"
                     /locus_tag="PLB65_04235"
     CDS             4585..5649
                     /gene="rsgA"
                     /locus_tag="PLB65_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOQ77769.1"
                     /translation="MKQKDKKKTNRWVGKMKNVHLPDPEILDEIKEQAKFKTARISTK
                     ASQSYKEGLKLVQGRIMEIKSNYRYIVEINGELHNAKMSGRLKQFLYSSHKIAAVGDY
                     VAVDVAIVSDKRIEKILPRKNALIRYGTGSFQKEIILAANIDQVIITSSWLKPRLKAG
                     LIDRYLCIVALQGLQPIIVINKIDLLDDEQELESQTAYYKAMGYPLLYTSVVSGTGMN
                     ELKELLVNKDSVFTGHSGTGKTSLINYLEPSLDLPTAEISNYNEKGKHTTSQSILIPW
                     SFGGHLLDTPGIKTITLHSEDKEYIPKIFPGFENLHNKCKFRDCSHIHEENCAVIEAV
                     ESGIIPIERYDSYQYLYESL"
     gene            5649..6503
                     /locus_tag="PLB65_04240"
     CDS             5649..6503
                     /locus_tag="PLB65_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOQ77770.1"
                     /translation="MQNFAVYINPDFSHKKNIYTLLEKLKDNSEINFFSIDSIPDLPK
                     ELFKPMPKPANRKHIDCILVFGGDGTILKAKDIALLTGAPILGINLGYLGFLSESVLP
                     EIASSIENLKQGKYRLLQRMLINCRLKREGKIIYEALALNDAVIYKAESPGLIHIRIK
                     ASSRYVFDTRCDGIIAATPTGSTAYSLSAGGPILAPEMKAIVLSPLNPHILAIRPMVF
                     PSTELLTMKVYGLRQAALLQIDGQNVMPIQEGDEVLVTSSQRSVSFIKLSNRTFYQIL
                     RRKLNLGK"
     gene            6503..8191
                     /gene="recN"
                     /locus_tag="PLB65_04245"
     CDS             6503..8191
                     /gene="recN"
                     /locus_tag="PLB65_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOQ77771.1"
                     /translation="MLTELYLQNYLMLQETRLPFDKGLTVITGETGAGKSILLGSISL
                     IFGDNLPGIEAFDKEKPIYLEATFSIKDEILTNILAQDGIEKEDELIVAREISTNNRS
                     TYFLNGRKVGVTLIKELKNYLLDFHHQRDQQRLLSSAYQLDLLDAYGGLFTLREDYAN
                     LYKEIKSDLKNLQELKSTAEKQKQLRELYRFQYEELENAKLHINEDVELQNEYELLSH
                     TLEITELCDEITADLLENENSIFDILNGYLNKLNHFEHLHKEIRNAKDNIHQALENLQ
                     EAANNLSSSRENISFEPQRLENIQARLDLINSLMYKHKVRNIEELLSLFEERSTQISS
                     LENSEKAIAELEENLNNNFTLLQKKGEELSRKRQQASKNLSNELQKNIRSLSIPNGTL
                     EIRIDKKTQSNFVISDYLSAASESGEDTVEFLFSANPGFEQKPLSAVVSGGELSRILL
                     AIKKVLAERLEPKLIILDEIEVGIGGKTAERVAEFITELSQKQQILCITHLAQIAARA
                     DKHLAIEKFTRNEKSMIELHELNAEETLQEIARMLSGSLSSKALAHAAELLKKI"
     gene            8207..9130
                     /locus_tag="PLB65_04250"
     CDS             8207..9130
                     /locus_tag="PLB65_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77772.1"
                     /translation="MDRRTKRRVKGIAIFIVFLAIAASLFEWQNNANKHNSYDFKAAQ
                     NEYRDLKFVNKAQISFVSSDVAKAQTEIENLIAKYSTKQIRKQSEGSYGAYIFSVPQK
                     DLPELVEKLRSFGTVVSKTEQVDTALVNIDYESEFARLQAYEREQADLNNVRFPTDAQ
                     NKRKEALHTLIQETRTNLDKLKDVQSVLLYVSLIPQQKSGSIGITIKTLAIDFFSWLV
                     VFSVGAVLVYFGTRLLMYFLAALGIKGIGSSGVGSSYNYGGYGNYYNRYYSRYGYGNR
                     KRKVKRIYKDKRTSSNSEADNNNNNNSEEGS"
     gene            9134..9574
                     /locus_tag="PLB65_04255"
     CDS             9134..9574
                     /locus_tag="PLB65_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOQ77773.1"
                     /translation="MALLPWQIWMIIGIGFIIIEIFDPAFFFISLGIGAIITSLLSLI
                     PFMGRNIAFQILVFAVLSFISFLFMRKLGKKLLANTGSNTNVFALKGKNGIVTKEIPL
                     EGKGYVKIGGEEWPAVEVDQKAIELGAKVIIEGIDGNKVIVRKI"
     gene            complement(9589..10236)
                     /gene="nth"
                     /locus_tag="PLB65_04260"
     CDS             complement(9589..10236)
                     /gene="nth"
                     /locus_tag="PLB65_04260"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOQ77774.1"
                     /translation="MEQLKEHFHSVKTPVVDLIKIKTDDPFKVLVATILSARTKDETT
                     AKVAEKLFQKVNKAEDFDKLTYEELDKIITPVGFHRVKAKHLKQLPKVLKEKFNGKIP
                     EEIDELMVIPGVGRKTANLVRAVAFQKPAVCVDVHVHRICNRWGYVKTKTPFETEMAL
                     REKLPDKYWLNFNSYVVAFGQNLCTPRRPQCERCSIIEFCEWGKEHSPIIQKENK"
     gene            10442..11137
                     /locus_tag="PLB65_04265"
     CDS             10442..11137
                     /locus_tag="PLB65_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOQ77775.1"
                     /translation="MCGRFAQVITHKELQKLEAELKIKKSSDQLEFNYNVAPTATVAA
                     VVSQADFRYLGFFRWGLIPSWRKEIPEQALINIRSETILEKPSFKASFLRRRCLIPVN
                     GFYEWRKTDKQPFFIKAKDSELLYLAGIYDSWHSPDGSYIPSLGIITTSANPYLQALH
                     NRMPLLLSPAVYDLWLDPNLNNPQELQPLLSPSSEIELEMYPVDKRINSPQNNDADCL
                     KPIKLSTAKDNYL"
     gene            11146..12228
                     /locus_tag="PLB65_04270"
     CDS             11146..12228
                     /locus_tag="PLB65_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ77776.1"
                     /translation="MERWKQHILVVDDDVAILDCIRMSIKKDIPCQITTTSDSLEALE
                     LLKNNVYNVVIADINMPNLSGFQLLEFISTISPNTPVILITGSTEPEKLRIAIQLGAH
                     DILRKPFELSDLHMAINNALQKNALLLQNEVYRLNLEHIVQQRTQELYAAKSKLEKNY
                     LNTIHAMVNAMEANDIYIRGHSERVTVLSIMIGKLLNLSAEELNLLRVGALLHDLGKI
                     GIYDTVLNKKDNLTPSEFSLIKQHPVIGAQIVRPIGLPEAVYNIILQHHEWFGGGGYP
                     YGLTQENISLLARIVTIADSYDAMTSKRVYRHNLEPNAARTEIINNIGFQFDPDIGLL
                     FFEKFCQITEPLSDTPAIQNLLSELI"
     gene            12259..12915
                     /locus_tag="PLB65_04275"
     CDS             12259..12915
                     /locus_tag="PLB65_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HOQ77777.1"
                     /translation="MVQVISATEAAAMVKPGSRIAIGGFLAVGAPETIIDAMVENENN
                     NLHMIVIASDWENRGVGKLIVNRMVTSAQVSHMGTNKTIQAQMNAREIEIELIPQGTL
                     MERVRAFGAGLGGILTPTGLGTIVEEGKQIISIEGVDYLLEPAIPSDFALIKAHKADK
                     LGNLTYRKTARNSNPIMAMAGRITIAEVDEIVEIGELDPEEVITPGVFVNYLVLAKEN
                     "
     gene            12917..13570
                     /locus_tag="PLB65_04280"
     CDS             12917..13570
                     /locus_tag="PLB65_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HOQ77778.1"
                     /translation="MALDKRAMIGARIAKEFEDGDYVNLGIGLPTEAANHIPEGIQVI
                     FQSENGLLGAGPKPPEGQEDEDMINAGGGYITCIPGASFFDSATSFAIIRGGHIDATV
                     LGALQVDQNGNLANWLIPGKMVPGMGGAMDLVTGAKKVIVAMEHCDKYGNSKILKQCS
                     LPLTAKGKVNLIITDMAVIEVLPDGLYLREIAEGLTIDDVVNATEADLIIPERVITF"
     gene            complement(14063..15385)
                     /gene="hflX"
                     /locus_tag="PLB65_04285"
     CDS             complement(14063..15385)
                     /gene="hflX"
                     /locus_tag="PLB65_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOQ77779.1"
                     /translation="MQKEDFNGLEEYELEPEVWEEVEKTEQTAFLATIGTPLETDKDI
                     EESLNELERLAETAGIDVLGVYRQRRSTPDRATYFGKGFLTELSQKMMQAQADLLIVN
                     DELSPSQARNIERDFQIKVIDRTEVILYIFHNRAKTREAKLQVRLAELEYQLPRLKRL
                     WEHFDKERGGIRNKGGTATRGMGEKQIEIDKRLITDNIRKINKAIESIQHQKETQRKQ
                     REKARKICLVGYTNAGKSTLFNYLTQAGVLVEDKLFATLDSTSRQLKLSTGYPVVLSD
                     TVGFISKLPHHLIASFKATLMEVQDADLLLHIVDVSDERFEYYIQQVNTVLQQIGADS
                     IPQIIIFNKIDKVDSMFPAVVKRRYPEAILISAVNGTNIDKLIAKIEEVLLGNKNLKL
                     KIPYDKTSLVSKLHDIAEILNEDYKEDGIYLEVGISKDDIYLVENYVI"
     gene            15704..16852
                     /locus_tag="PLB65_04290"
     CDS             15704..16852
                     /locus_tag="PLB65_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HOQ77780.1"
                     /translation="MKKHLLFILILGCTLVSLAAQATKEEENDDKKVSAKFEININED
                     PINELSTYNKPYMGIRHKDITLPKAKELNYNGYAGILIVDVIPGSPAWQHHLQEEDIL
                     FCVDGKEITDSATFHKIIKNYSAGDKVILGIFRDGKTNEVEFTFGSRNKTELKIEEKT
                     CKNKSAGYRGITWLPMFVFLDMEDVNNLIASDTLSFGKLNEDGSLQQGIAGKFSIGKG
                     FFLGGQYSSYKDVKKHHNQTNPEYQLWMKYKNQMGGVTLDKRIPITKNFISSAGLMVG
                     GAKQIMEFQNSNSNFNWDSLPSTITHSNNTHFEITKGYLTVQPRFELMYRFLPWLALR
                     AEIGYAYSYNLTNYWKVTNIDGDPVEIKGSPDTNFQGLTLSIGPWLGF"
     gene            17151..17933
                     /locus_tag="PLB65_04295"
     CDS             17151..17933
                     /locus_tag="PLB65_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOQ77781.1"
                     /translation="MKRFSLIIITFLVLSSMLWAEEQPSYAYKYLASYPQTARQAITA
                     PLSWQGGDWLKAGAVVLSAGALYLADEDIQHYSQNSRNDGTDRLMNIASNFGEYRIVA
                     PALGIGILGAYLQNDKKALDTGLLSMKSFVLSSVICQGLKLATQRNRPSEGLGKKFWQ
                     ESKISFDNDSFPSGHSTVVWSIAPILADQYQESNWIPPVSYGIATLTSLSRIYNDEHW
                     ASDVLAGAAIGYFSAKLVSTSTPRLILAINPAIHGAGFSYKF"
     gene            complement(18026..20149)
                     /locus_tag="PLB65_04300"
     CDS             complement(18026..20149)
                     /locus_tag="PLB65_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77782.1"
                     /translation="MNRCYLLFSLLLIVFPLSAIDIPFSDQAVMTDTNLIGDFVRIDP
                     DDSQAETLATKVWVWQEEGKLMVHFSCAINENFMPGYPASRDSEIQADYLRVQLVTLP
                     DANFAYLFGAYSSGHLVDGIRKDDMSVDYQWDSDYDYESSYNDSLWTVTFSIPLGSLR
                     FKQELPYNWGLIFTRFHQSTKEYYSSPYANTDSKLEYFKAVHPIVLHEKVKRELDMKI
                     KPYFVKSYDLINKTDSFDPDMMGLDIALNPGQQTKMKFSFNPDFSDVPPDNAQDVYNN
                     DIPTMYSENRFFFVEDLDAFGLRESVFYSRNINKPSFAYKATGNVGTTRWGILGAKDE
                     KVVEDGEVQNNDDYFQVLSLIPSYSIFTMGNALISRMNKGYYNHAYFGNYDLKLGKEL
                     MLNTEVTGSIKSNDEGEEDKVLKGYQLFSELNFSPGNFDNYIYYQKVSKDIFLDAGYL
                     YYKDRQTYGISMDWNSNPGYGFIKKKRSFLSLDFSDWYIGDNTYNEYSWYGNFGIDLK
                     SGFNYSFNSNFGTVHDDLWEKHHYYGVSSSISRSEENWAHITLGMSYGDELVYVLNDT
                     YGKFGLVANASGNIAKMYSYSFDWQYIDFSYPKDNIVDYGDGSANVKLDDRYSICNVR
                     LNYNPNLKLCFTGGISYTNYALYGVYSDVQFYGNMRYEFKRDHFIYAGIKSSQSQDEK
                     STYSDPLGHFIKNSATAYMKLALTL"
     gene            complement(20215..21006)
                     /locus_tag="PLB65_04305"
     CDS             complement(20215..21006)
                     /locus_tag="PLB65_04305"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOQ77783.1"
                     /translation="MKHYLVYYIQANLLQLKRMVVYNKSFLLDVLADLTGIMLALFFY
                     QILYLNVGEGLRISIEDLYILVLSVKLTGDLYNLLFGSGVHSLPSLIQYGSLDSILIK
                     PMNLFFMLFCSTLNWKYFVNLIIDVVLFVIIFTQYNIAISVMSICLYLIMIGLATMVY
                     TAFNILIYLGSIIFVRMDAFASLISSFFSLSTYPAAIFRASYLKWIFIYVIPVLVIGN
                     FPMMAMKGGYGYQLYIAASMCAVVFVIAAYWGTRSMLRHYKSASS"
     gene            complement(21003..21797)
                     /locus_tag="PLB65_04310"
     CDS             complement(21003..21797)
                     /locus_tag="PLB65_04310"
                     /inference="COORDINATES: protein motif:HMM:NF017946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HOQ77784.1"
                     /translation="MSRYYTALGIQTIKEAAAYRTNFILSFLVVLIPLGGQLLLAKFI
                     YSGGYQIGAWKLDQLLLYYIIATIVSEFFVIGTWWDIQNDIREGGLNYHLLKPYSYFW
                     HNFVVYYALKAVYTIFAIIVAYLAFIAVSGNLVPQIKLQSIIQFIIMALIAAPLSFLI
                     TFYLSILAFYFTDIGFVMNFLGILLPFASGNILPLDIFPRGIRGLLLKLPSAYLVYHP
                     SCILTGNYAWRSYWQIVLEALMWLIVFIMTTQVAWSQGRKKYEAIG"
     gene            complement(21790..22746)
                     /locus_tag="PLB65_04315"
     CDS             complement(21790..22746)
                     /locus_tag="PLB65_04315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ77785.1"
                     /translation="MENSRTIRIESLWKKYRVKTSVSLFRPVYKEIEAIRGISLCHNL
                     DHNLGIFGKNGAGKTTLVKLLTGILHSSSGEILVLGHIPYKLKPEFKKQIGLFQGGKE
                     QLIWDLPAIQSLELSRYIYGFSRDEFKKRITHFSQALDIGDELDQPLRNMSLGQRCKM
                     ELIYSFIHLPKIVFLDEPTSGLDIITRSKLRGFINFCHKEYGICFIITSHNVKDITDC
                     CNSCIVLDNGEIIYQGDTTFLLEKHQHEVITLSAKSNDVAREIAEKFGGTTVEDEEVV
                     FESTGNHSKQLICQILAQYEISDFSIKKKDDEEIITEILKNE"
BASE COUNT         7309 a         4379 c         4303 g         6869 t
ORIGIN      
        1 aataaaaaaa gtcccagcgg gacgacagat actaaatgaa ctttaaatta ttagtcctct
       61 taatccttaa atccttgttt ctaaaatcaa atctgtgcga gataaaaata cacacacttc
      121 ttcctattca acaaaatgct tgactgaata agataggtag taagcgtagg tttataatgt
      181 atctggaaat aataaaatac gaataccaag gttaaaaccg taatgccaag aagtatagcg
      241 cgtatttcta tctggaaaaa ggcagttcct ttgttggtta ttattttaag ttcttttttg
      301 ggtattgcct cccgcaaatt cggatatatt ttacctcctt ttttggcaaa atatactgga
      361 gacacaatgt gggctttggc tttttgtgct ttattccgga tgctttttac tcggcaaaga
      421 aattgggaaa ttgccataat tgcttttgat ttttcttgct taatagaatt gagtcaattg
      481 tggcatcctg tttggttgga tagaatccgc tcaacagttt tcggtggttt actatttggc
      541 ttcggttttc gctatataga tcttttttgt tatgcagcag gctgtgtgtt gggtttattt
      601 attttttctt taattccaga aggttagtgg agattttttt acacaaaaaa actgttaacc
      661 ctatcttttt gattgacgga aatggtttat ttacataaaa tggaacagaa tttgtattat
      721 ataaataagg atataacctt ggaggttaaa aaaaatgctg caaggaactt atgttgccct
      781 aattactcct tttaaaaatg gcagtattga ttggactgct ctggaaaatc ttatccagtt
      841 tcatctggaa aatggaacaa ccggtatttt gctattagga acaaccgcag aaacgagtgc
      901 tttggcttct gatgaaaaag atgccctatt acgctttgcg atgaagaaaa ttgatagtaa
      961 actgcctgtt atgattggaa caggaaccaa taatcttcat cagaccttag cttccaccca
     1021 aaaagcaaaa gaactgggtg ccgattatgc tttaattatc actccctatt acattaagcc
     1081 cacacaaaaa ggtatgctcg aatattatgg tatcattgct tccaaagtgg atattccgat
     1141 tattatttat aatgtccccg gtagaacaag tgtaaatatg aatgctgcta caacggtaga
     1201 attggcaaaa acctatccca atatagttgg agtaaaagat gccacaggaa atatagttca
     1261 ggccactcaa ataattagag atgctcccga caacttttct ttgatgagtg gagaagatgc
     1321 tatgaatctt cctcttattg cgattggagg aaagggaaca atttctgtta ctgctaatgt
     1381 agctccccgt ttggtaagtg aacatatttc tacttgttta caaggtgatt ttgttacagc
     1441 ggcaaaacaa catcaatatt tagctcgctt aaatgaaatg atgtttattg aaactaatcc
     1501 tattcctgct aaagaggcac tggcgatgat gggaaaaatt aatttagctt tccgcagtcc
     1561 aatgtgtcct ttattggaaa ctaaccgtgc aatgttaaaa caatgcttgc aggactataa
     1621 cctattgtaa gaggaacaaa tgaaacaaaa agagctaatc tatggctttc aattaattga
     1681 aaagcgcgaa attaaagaga tatcaaccac cgcccacctc tacaaacata taaaaagcgg
     1741 cgctgaactg atttattatg aatgtgatga tgacaataag gtatttatga ttgggtttaa
     1801 aaccgttcca gaagataata ctggctgtcc tcatatcttg gaacattcag ttctaaatgg
     1861 tagtagaaac ttccctgcaa aaactacttt tatggaactg gtgaaaggta gtatgaacac
     1921 ttttatcaat gctatgactt ttcccgatat gacttgttat cctgtagcca gtacaaacga
     1981 taaggatttt attaatttaa tgagagttta cctggatgca gttctctttc ccaatattta
     2041 cgagcagccc aatattttgc atcaagaggg atggcatttg gaattaactt cggaagatgc
     2101 tcctctcaat tatcgcggcg ttgtttacaa tgaaatgaag ggtgccttat cttctccaga
     2161 aaatattatt ggccgtaaaa gcctccatgc gcaatttccg gattctccat atagttttga
     2221 aagtggagga gacccagatt ctatccccga attaacttat gacaatttta ttgccttcca
     2281 taaaaaatac tatcatccct cgaacagtaa aataactcta tatgggaatc taaatctggc
     2341 agatacattg aaaataattg atgcagaata cctttctcaa tttgaggaca gcaaacaaaa
     2401 agtgcatctt ccattgcaga agcctttcgc taaagctaaa gtaacgaaaa tggacattcc
     2461 cttggaagaa ggtaaagata ttgaagagca atattaccta tctttgaact ggacttatgg
     2521 cttgattacg gataaatatc tgcctcaagc attgaaagct ttagcagaaa tgttgatgga
     2581 tacacccgct tctcctctga aaaaagtaat tcgggattcc ggctatgcca aagatagctt
     2641 cgtttatgta cataacgata ttctgcaacc tacaatcagt ttaatctgta aacaagtgaa
     2701 acaggaaaat attgaacctt tggtaaaact gatcaaacaa gaactggaaa atatcgtgaa
     2761 aaacggcttc gataaaaaac ttgtggaagc cataatcaat aagctggaat tttctttgcg
     2821 cgaagctcag tatcaatatt atcccaaggg tttaatttat gctctaaatt ctcaagcgct
     2881 atggatgcat acaggaaatc caatggatta tcttgctttc gaacctatgc tgaaagaact
     2941 gcgtcgcggt ttagaagagc cctattttga aaatttgatt gagaaggtct tgctttataa
     3001 taaacactct catcaaattg tcttcgagcc cgtacctggt ttgattcaaa aaaaggaaca
     3061 agaaacagct gctaaattag cagaattaaa aaagaaaatg agcaaaaagg aaattgccaa
     3121 attgctcgag ttcaatcaac aactaataca atggcaacaa gaaccagaga aaaaggaaaa
     3181 cttggaaaaa ataccagttc tttccttatc ggacttaaac cccctttcta aacattatcc
     3241 tattgaagag gaaattaaag cggattatac tctgctgaaa catcctctaa ctaccaacgg
     3301 gatagtatat tttaaagcat attttgatat tgctcatgct gaagaagaag acttgccctg
     3361 gattaaacta tacactcagt tggcagaatg gatgaatagc gaaaactata attttgccca
     3421 gcgctctacg gaaattgata ttaataccgg cggtataact ttagatatag atttatataa
     3481 caattaccaa actccggatg atatcctgcc taaaatagtt ttagcgggaa aagcggtaag
     3541 cactaaattt ggaaagctga tggagcttgc ttctgaatat gctctaaaac caatgtttga
     3601 tgatccgcaa cgcattaaac aactgctggc agaacttaaa gctaaaactg aaacaatgct
     3661 accttttcaa ggacatagaa ttgccattca tagaatgctg aaacccattt ctcacctcta
     3721 tcattggaga gatatttcca gtggcttagg ttattatcat ttcctttgtg atctcgtgaa
     3781 taatatggat tccgctattg atgatatcat cgaagagcta aactggatca aaaagacctt
     3841 ctttactacc cataatttaa ttatcagcat cacagccgat gaggaagata ttttaccaag
     3901 cacaaatgaa ttggcaaatc taatctcctg tgtatctggg gaagcatttg cccccattaa
     3961 aaatctgtat gctatccgcg attataacga agggatttat gcacctatcc aagttcaata
     4021 ttgtgcaaaa gggggtaact tctttcgcag aggttattcc tacagtggaa aattgagagt
     4081 actgaataat attctccgca gcaactattt acacaaagaa ttacggatta aaggtggagc
     4141 ttatggtaat atgagtgatt tcacacccgg tggttatgta tattttgtct cctaccgcga
     4201 tcccaattta agagaaacct tggaggtctt cgattctgtt cctgaattct taagaagttt
     4261 tgattgcgat aagcaagaat tcgataagta cataattggt gaaatatctg ctctggatta
     4321 tcctttaacc cctgaaggaa caggagaaaa agcagacgaa agctatatta ccggatttag
     4381 ctatgaagat agacagcaaa ttagagacga agttctatct gctaaaaaag aagatatcag
     4441 aaactatgct gatctcgttg aagcagtaat gctaagaaat cactatgctg ttttcggctc
     4501 ggaaacaaaa gttaaagagg cagcagatat atttgatgcc ataattccag ctctgaaata
     4561 aacttatccc tggcttggga ggaaatgaaa caaaaagata aaaagaagac caaccgctgg
     4621 gtaggcaaaa tgaaaaatgt tcacttgccc gatccagaaa tcttagatga aattaaggag
     4681 caggcaaaat ttaaaaccgc ccgcataagc acaaaagcaa gccagtcata taaagaaggg
     4741 ctgaaacttg tgcaaggcag aattatggaa attaaaagta attaccgtta tattgttgaa
     4801 ataaacgggg aattgcataa cgccaaaatg agtggacgct tgaaacaatt tttgtattca
     4861 agccataaga ttgctgccgt gggtgattat gttgccgttg atgttgccat agtttccgat
     4921 aaacgaattg aaaaaatatt gccacgcaag aatgctctta ttcggtatgg gacaggaagc
     4981 tttcaaaaag aaatcatcct ggctgctaat atcgatcagg taattatcac ttcctcctgg
     5041 ctgaaaccaa gattaaaagc aggattgatt gatcgctatc tttgtattgt tgcccttcaa
     5101 ggtttacaac caatcatagt aattaataaa attgacctct tggatgatga gcaggaactg
     5161 gaaagccaaa ctgcctacta taaagcaatg ggctaccctt tactgtatac ttcagtggtt
     5221 agtggcacag gaatgaatga actgaaagaa ttgctggtta ataaagattc cgtctttact
     5281 ggtcactcag gaactggaaa aacttctttg ataaactatc tggagccatc cttagatctt
     5341 cctactgctg aaattagcaa ctacaatgaa aaaggaaaac acactacttc gcaatcaata
     5401 ttaattccct ggagttttgg aggccacctt ctagatacac ccggtataaa aactatcact
     5461 ttacatagtg aagataaaga atatattcct aagatcttcc ccggttttga aaatctacat
     5521 aacaaatgca agtttcgcga ttgcagccat atccacgaag aaaactgtgc tgttattgaa
     5581 gctgtggaaa gtggcattat tcccattgaa cgctacgata gttatcaata cttgtatgag
     5641 agtttataat gcagaacttt gccgtatata ttaatcctga tttcagccat aaaaagaaca
     5701 tctatactct gctggaaaag ctgaaagaca attccgagat aaatttcttc agtatagata
     5761 gcattcccga tttgcctaaa gagctgttta aacctatgcc caaaccagct aaccgtaaac
     5821 acatagattg tattctggtt tttggtggtg atggaactat cttgaaagct aaggatatag
     5881 ctcttttaac cggagctccg attttaggaa ttaaccttgg ctatttgggt tttctttccg
     5941 aaagtgtttt accagaaatt gcctcttcca tagaaaacct gaaacaggga aaataccgtt
     6001 tgctacagag aatgctgatc aattgccgtt taaaacgcga aggcaaaatc atctatgaag
     6061 ctttggcttt aaatgatgcc gtaatataca aagcagaaag ccctggttta attcatatcc
     6121 ggattaaagc cagtagccgt tatgttttcg acacccgttg tgatggaatt attgctgcaa
     6181 cgcctactgg ttcaactgcc tattcattgt cagctggagg ccctattttg gctccagaaa
     6241 tgaaagccat tgttctttct ccgcttaatc ctcatatttt agcaattagg ccaatggtct
     6301 ttccctctac cgaactttta actatgaagg tttatggctt aagacaagct gctctgttac
     6361 aaattgacgg acaaaatgtg atgcctattc aggaaggaga tgaagtttta gtaacatctt
     6421 cacaacgcag tgtttctttc atcaaacttt ccaatcgcac tttttatcag attctgcgtc
     6481 gaaaattgaa tttgggtaag tgatgcttac tgagctttat ttacagaact atctaatgtt
     6541 acaggagacg cgtttacctt ttgataaggg tttaacggtt atcacaggtg aaacgggagc
     6601 cggtaaatct attttacttg gctcaatttc ccttattttt ggggataact tacctgggat
     6661 agaagcattc gataaggaaa agccaattta tctggaagct accttttcta taaaggatga
     6721 gatattaact aatatccttg cccaagatgg aatagagaaa gaagatgagc taattgttgc
     6781 cagagaaata agcaccaata atagatccac ttattttctt aatggacgca aggtaggggt
     6841 tactttaata aaggaactga agaattatct gctggatttt catcaccagc gagatcagca
     6901 gcgattgctg tcttccgctt atcaacttga tttgttggat gcttatggag gactttttac
     6961 tctcagagaa gattatgcca atctctataa agaaataaaa tcggatttga aaaatctgca
     7021 agaactgaaa agcacagcag aaaagcaaaa acagctacgc gagctctatc gctttcaata
     7081 tgaagaattg gaaaatgcca aattgcacat aaatgaagat gttgaattgc aaaatgaata
     7141 cgaattgctt tctcatactt tagaaataac agaactctgc gatgaaatta ccgccgacct
     7201 cttagagaac gaaaacagta tctttgatat attaaacggt tacttaaaca aattaaacca
     7261 ctttgaacat ctccataagg aaattagaaa cgctaaagat aatatccatc aggcattgga
     7321 aaacctacag gaagctgcta ataatctttc tagcagcaga gaaaatatct ccttcgaacc
     7381 gcaacgcctg gaaaatattc aagcccgctt agatttaatc aattccctta tgtacaaaca
     7441 taaagtgcgt aatatagagg aattactgag tctttttgaa gaaaggtcta cccaaatatc
     7501 ctctctggaa aatagcgaaa aagctattgc tgaattggaa gagaacttaa ataacaattt
     7561 cacgctgtta caaaaaaagg gagaagaact ctctcgtaaa agacagcaag caagcaaaaa
     7621 ccttagtaat gaactgcaaa aaaatatccg ctctttaagt attccaaatg gaactctgga
     7681 aattaggatt gacaaaaaaa cgcaaagtaa ttttgttatt tctgattatt tatctgctgc
     7741 ctcggaaagt ggagaagata ctgtggaatt tcttttttcc gccaatcctg gttttgagca
     7801 aaaaccttta tccgccgtag ttagtggagg tgaattatcc agaattctgc tagcaataaa
     7861 aaaggtgctg gcagaacgtt tggaaccaaa actgatcatt ttggacgaaa tagaagttgg
     7921 aattggaggc aaaacagcag aaagggtagc tgagtttatt actgagctat cccaaaaaca
     7981 gcagattttg tgtatcactc atctggctca aatagctgcc agagcagata agcatctggc
     8041 aattgaaaaa tttaccagaa atgagaaaag tatgattgaa ttacacgaat tgaacgctga
     8101 agaaacttta caggaaattg ccagaatgct ttctggttct cttagttcca aagcactggc
     8161 tcatgcagct gaattactaa aaaaaatata actgctcgag gttaatatgg atcgaagaac
     8221 aaaacgcaga gttaaaggaa tcgctatttt catcgtcttc ttagcaattg ccgcttcttt
     8281 atttgaatgg caaaataatg ccaataaaca taactcttac gatttcaagg cagctcaaaa
     8341 tgaatatcga gaccttaagt ttgtaaataa agctcaaatt tcatttgttt cctctgatgt
     8401 ggctaaagct caaaccgaaa ttgagaacct catcgctaaa tattctacta aacaaattag
     8461 aaaacaaagc gaaggctctt atggtgccta tatttttagc gttcctcaaa aagaccttcc
     8521 tgaacttgta gaaaaattac gcagtttcgg aacagttgtt tctaaaacag aacaggtaga
     8581 cactgctttg gtaaacatag attacgaaag cgaatttgcc cgtttacaag catacgaaag
     8641 agaacaggca gacctgaata atgtccgttt ccctactgac gctcaaaata aacgaaaaga
     8701 agcattacat actttaattc aggaaactcg taccaatctg gataagctga aagatgtgca
     8761 aagtgttctc ctatatgtat ccttaattcc acaacagaaa agtggttcaa ttggcattac
     8821 tataaaaact ttggcaattg atttcttcag ctggttagtt gttttttccg ttggagctgt
     8881 acttgtttat tttggaactc gtttattaat gtatttcctt gctgccttag gaataaaagg
     8941 gattggttca agcggagtgg gcagttctta taattatggt ggctatggaa attactacaa
     9001 ccgctattac agccgttatg gttatggaaa tagaaaacgg aaagttaaac gcatctacaa
     9061 agataaacgc acttcttcta attctgaagc ggataacaat aacaacaata attccgagga
     9121 aggaagttaa catatggcgc ttttaccttg gcaaatatgg atgataattg gaatcggctt
     9181 tatcatcatc gagatatttg atcctgcttt tttctttatt tctttgggaa tcggagctat
     9241 tattacttcc ctgctatctt tgataccctt tatgggtaga aatattgcct ttcagatact
     9301 cgtttttgca gtcttaagct tcatttcctt tctctttatg cgtaaattgg ggaaaaagtt
     9361 attagccaat acaggtagca ataccaatgt ttttgcctta aaaggaaaaa atgggatcgt
     9421 aactaaagaa attcctttag agggaaaggg atatgtaaaa atcgggggag aagaatggcc
     9481 agctgttgaa gttgaccaaa aagctataga attaggagct aaagttatca ttgaaggaat
     9541 cgacggcaat aaggttatcg ttagaaaaat ttagtccgcc tcttctcttc acttattttc
     9601 cttttgtatt atcggtgagt gctctttccc ccactcacaa aattcaataa tagaacacct
     9661 ctcacattga ggtcttcgcg gtgtacagag attttgtcca aaagcaacca catacgaatt
     9721 gaaatttagc caatatttgt ccggtaattt ctctcgcaga gccatttctg tctcaaacgg
     9781 tgttttagtt ttcacatatc cccagcggtt acaaattcta tgtacatgaa catctacaca
     9841 gacagcaggt ttttgaaaag caacggcacg aacaaggtta gcggtttttc ttccaactcc
     9901 tggaattacc attagctcat caatttcctc ggggatcttt ccgttaaatt tctccttcag
     9961 cacttttggt aactgcttta aatgtttagc tttaactcta tgaaatccaa cgggagtaat
    10021 tattttatcc agttcttcat aggtaagttt atcaaaatcc tctgctttat tcaccttttg
    10081 aaacagcttt tctgcaactt tggcagtagt ttcatctttt gttctcgcgc tcaaaattgt
    10141 tgccaccaac actttaaatg ggtcatcagt tttaattttg atcagatcta caaccggtgt
    10201 tttaacactg tggaaatgct cttttagctg ttccatcacg atatctatat cgctattttg
    10261 cataatatct ccattcttat tagtcttttt ttgtggacgc tgcctattca gtatttaaag
    10321 gttctttgta tacaggaaga caaaaaaaga ggatttatct ccccaaatat ctaagaggta
    10381 aaaatattta ttgcctaaaa tatggctttt ataaatcttg tcaacttgaa aggagatata
    10441 aatgtgtgga cgctttgccc aggttatcac tcataaagaa ctacagaaat tggaagctga
    10501 actgaaaatt aaaaagagtt cagatcagct tgaatttaat tataatgtag ctcccacggc
    10561 tacagttgct gctgttgttt cccaagcaga ttttcgatat ctgggttttt tccgttgggg
    10621 tttaattcct tcctggcgaa aagaaattcc tgagcaagca cttatcaata tccgcagcga
    10681 aacaatatta gaaaagccaa gttttaaggc atctttttta agaaggcgtt gtcttattcc
    10741 cgtcaatggt ttttatgaat ggcgaaaaac cgataagcaa ccctttttta ttaaagcaaa
    10801 agatagtgaa ttactttatc tggcaggaat ttatgacagc tggcatagtc cggatggtag
    10861 ttatatacct tctttaggaa taattaccac ttctgccaat ccttatcttc aagctttgca
    10921 taatcgaatg cctctattat taagcccggc tgtttatgat ctttggcttg atcctaacct
    10981 aaataacccc caggagttac agccattgct tagccccagt tctgaaatcg aactggaaat
    11041 gtatccagtt gataaaagaa ttaatagtcc ccaaaacaac gatgcggatt gcttaaagcc
    11101 aattaagcta tctaccgcta aagataatta tttatagagt aatatatgga gcgctggaaa
    11161 caacatattt tagtagtaga tgacgatgta gctattctgg attgcatccg gatgagcata
    11221 aaaaaagata ttccttgcca aataactact accagcgatt ctctggaagc tttggaactg
    11281 ctaaaaaata atgtttataa tgttgttatc gctgatatta atatgcccaa tctttctgga
    11341 tttcagcttt tagaatttat cagcacaatc agcccgaaca ctcccgtgat tcttattact
    11401 ggctctacag aacctgaaaa attgagaatt gctatccaat taggagcaca cgatattctg
    11461 cgtaagccat ttgaattatc cgatttgcat atggctatta ataatgcgct tcaaaaaaat
    11521 gctcttctct tgcaaaatga agtataccgt cttaatcttg aacatatagt tcagcaacgc
    11581 actcaagaac tttatgccgc aaaaagtaag ctggaaaaga attaccttaa taccattcat
    11641 gcaatggtta atgccatgga agcaaatgat atttatattc ggggtcattc cgaacgcgtt
    11701 actgtgctaa gtattatgat cggcaaactt ttaaatttga gtgccgaaga attaaatcta
    11761 ttacgagtgg gagctctatt acacgattta ggcaaaatcg gcatctatga taccgtcctg
    11821 aataaaaagg ataacttaac ccctagcgaa ttttctctca taaaacagca tcctgttatc
    11881 ggagcacaaa ttgttcgccc cattggttta ccggaagctg tgtataatat tatattacag
    11941 catcatgaat ggtttggagg gggtggttat ccttacggtc ttacccaaga aaatatttct
    12001 cttttagcca gaattgtcac tattgctgat tcctatgatg cgatgaccag taaacgagta
    12061 taccgtcata atcttgaacc aaatgcagca aggacagaga taattaataa tataggtttt
    12121 caatttgacc cagacatcgg tttactattc tttgaaaagt tttgccagat tacagagccg
    12181 ttatcggata caccagctat ccaaaatctg ctctctgaat taatataaac agcatattaa
    12241 aatatacaag gagacaaaat ggttcaagtt attagtgcca cagaggcagc tgctatggtt
    12301 aaaccaggaa gccggattgc catcggcggt tttttagctg tgggagctcc agaaactatt
    12361 atcgatgcaa tggtggaaaa tgaaaataat aacctccata tgatcgttat cgcttccgat
    12421 tgggaaaacc gaggtgtagg taaactcatc gtaaacagga tggttacaag tgctcaagtt
    12481 tcacatatgg gaaccaataa aactatccaa gcccagatga atgccagaga aatagaaatt
    12541 gaacttatcc cccaaggaac acttatggaa cgcgttcgcg cttttggagc tggtttagga
    12601 ggtattttaa cccccactgg attgggcaca attgtggaag aaggaaagca gattatttct
    12661 attgagggag tagattatct tctcgaacct gctatcccca gtgattttgc cctcattaaa
    12721 gctcataaag ccgacaaatt aggcaactta acctatcgta aaactgcccg taacagcaac
    12781 cccattatgg ctatggcagg gagaatcaca atcgcagaag ttgatgaaat agttgaaatt
    12841 ggagaattag accccgaaga agtgatcact cctggcgttt ttgttaatta tcttgtttta
    12901 gcaaaggaga attaaaatgg cattagataa aagagctatg atcggtgcac gcatcgctaa
    12961 agaattcgaa gatggcgatt atgtaaacct gggtattgga ttgcctaccg aagctgcaaa
    13021 tcatattcca gaagggattc aagttatctt ccaatcggaa aatggactct taggcgccgg
    13081 acctaaaccc ccagaaggcc aagaagatga ggatatgatc aatgctggag ggggatatat
    13141 cacttgcata ccaggagcca gtttctttga ttccgctacc tcgtttgcca ttattcgcgg
    13201 aggtcatatt gatgccaccg ttttaggtgc cctacaagtt gatcaaaacg gtaatttagc
    13261 taactggcta attcctggaa aaatggttcc cggtatggga ggcgcaatgg atttggtcac
    13321 tggagctaaa aaagttatcg tagctatgga acattgtgat aaatatggca attccaaaat
    13381 tctgaaacaa tgctccttac ccctcactgc caaaggaaaa gtaaatctta ttatcaccga
    13441 tatggcagtt attgaagttc ttcctgatgg actttatctt cgagaaattg ctgagggatt
    13501 aactatagac gatgtagtaa atgccactga agcggattta attatccccg aaagagttat
    13561 tactttttaa ctaacttcgg attttaacct tttcagccat tccttccgtt ctgaaatagg
    13621 gaatggctgt ttttttgtgt acctgatctt gtttttatga gccacccacc tttcttgatt
    13681 cccgaacaca actcctcctt atcccgtccc tatcccatcc ctatcccatc ctcaacttac
    13741 cgttggggag gagtggatga taagtggagg tggacaggat tgtaacgatc tgttatttgc
    13801 acatttagga agaaaaatgg ctgggaagaa agaaattagt ccagcagaaa gccgagggct
    13861 tagagcggag agaaacaaaa tttcacccag attttgtata tataataggg ttattagttc
    13921 cttaaggagg acaggtatta gcgatgtttt tttaccggca aatccaattg cttcaattag
    13981 ctgtgtgcta tttataccac taaggcaacc gaagaaatag cttttatctt actatcttgc
    14041 tttatcttat tgatctatat cactatatca catagttttc taccaaatat atgtcgtctt
    14101 tactaattcc cacttccaga tagatgccat cctctttata atcttcgttc aatatctctg
    14161 caatatcgtg cagtttagaa accagcgagg ttttgtcata aggtatttta agtttcagat
    14221 ttttatttcc caagagcact tcttcaattt tggcaattag tttatctata tttgtgccgt
    14281 taacagctga aattaaaata gcttcaggat agcgtctttt tacaactgcg ggaaacatag
    14341 aatcaacttt gtcgattttg ttaaagataa taatctgggg aatagaatca gctccaatct
    14401 gttgcaacac tgtatttact tgctgaatat aatattcgaa gcgttcatcg gaaacatcca
    14461 caatatgtaa taataaatcg gcatcctgaa cttccattag agttgcctta aaagaagcaa
    14521 tcaagtgatg tggcaattta gaaatgaaac ctactgtatc tgagaggaca acaggatatc
    14581 ctgtggagag tttcagctga cgagaagtgg aatctaaagt ggcaaagagt ttatcttcca
    14641 ctaaaacccc ggcttgagtt aaatagttaa agagggtaga tttaccggca ttagtatagc
    14701 ctaccaagca tattttgcga gctttttcgc gttgtttgcg ttgggtttct ttttggtgct
    14761 ggatactttc gatagctttg tttattttgc ggatgttatc tgtaataagg cgtttatcaa
    14821 tttcaatttg tttttcaccc atacctcttg ttgctgtccc tcccttattt ctgataccgc
    14881 ctcgttcttt atcaaaatgt tcccataatc tttttaagcg gggcaattga tattccaatt
    14941 ctgccagacg aacctgtagc tttgcttcgc gcgttttagc acgattgtga aaaatatata
    15001 aaataacctc ggtgcggtcg ataactttta tctgaaagtc tcgttcaata tttcttgctt
    15061 gcgaagggct aagttcatca ttcacaatta gcaaatccgc ttgggcttgc atcatttttt
    15121 ggctgagctc ggttaaaaaa cctttgccaa aataagtagc acggtctgga gtacttcttc
    15181 tttgacgata aacccccaaa acatcaatgc ctgcggtttc tgctaagcgt tccaattcat
    15241 ttagcgattc ttcaatatcc ttatccgttt ctaagggtgt tcctatggta gctaaaaagg
    15301 cagtttgttc agttttttcg acctcttccc agacctcagg ttccagttca tattcttcca
    15361 aaccgttgaa atcttctttt tgcataatta ctttcctttt ttataacctc ctcttattta
    15421 ctatcaggta aatacgaaaa tgctaatttt tattttgtcg taattacccc agctgaagtt
    15481 ataacctata tatagaataa cgcagtaacc agaaaaggca aattttaaga cacaggatgt
    15541 gaaggtaagt taatatggaa aaacaaaatc ttgacagaat gttatctaat aattattgat
    15601 aatttttgtg gaacataatt gaaaacaaac gatagagctc agattatcac ataagctggt
    15661 attataaagt taatatataa aaatgcacaa gaggataaga ataatgaaaa aacatctttt
    15721 gtttatccta attttaggat gcactctcgt ttccttggca gctcaagcga ctaaagagga
    15781 agaaaatgat gataaaaagg tttcagctaa atttgaaatt aacatcaatg aagatcccat
    15841 taatgaatta tcaacctata ataaacccta tatgggaatt cggcataaag atattacatt
    15901 accaaaagca aaagaattaa actacaatgg atatgcaggc attttgattg tcgatgtaat
    15961 tcctggttct cctgcctggc aacatcatct gcaggaagaa gatattctat tttgtgttga
    16021 tggaaaagag ataacagatt ctgccacttt ccataaaata atcaagaatt acagcgctgg
    16081 agacaaggtg attttaggta tattcagaga tggaaaaaca aatgaggttg aattcacttt
    16141 tggctctcga aataaaactg agctgaaaat tgaggaaaaa acctgcaaaa ataagtccgc
    16201 tggctataga ggaataacct ggctacctat gtttgtattt ctggatatgg aggatgtgaa
    16261 taatttgatt gcttccgata ctttaagctt tggaaagcta aatgaggatg gttctttaca
    16321 acaaggtatt gccggaaaat tctccattgg taaaggattt tttttagggg gtcaatacag
    16381 ctcctataaa gatgtaaaga aacatcataa tcaaacaaat cctgaatacc aactctggat
    16441 gaaatataag aatcaaatgg gtggagttac tttagataaa cgcattccca tcactaagaa
    16501 ctttatttct tccgctggat taatggtagg cggagcaaaa caaataatgg aatttcagaa
    16561 ttccaattct aacttcaatt gggatagctt accttctacc ataacccact ccaataatac
    16621 tcattttgaa attactaaag gatatcttac agttcaaccc cgttttgaat tgatgtatcg
    16681 ttttctgccc tggctggctc ttcgcgctga aattggttat gcctatagtt ataatcttac
    16741 taattactgg aaggtaacta atattgatgg cgatcctgta gaaattaaag gttctccaga
    16801 tactaatttt caaggtttaa ccctttctat tggtccctgg ctggggttct aataactaaa
    16861 aagaatcacc tgtttgtggg tgtcgaatca ttgtctgaag aggcactaaa ggtatttaat
    16921 aaaggtgttt cagttgagca gatactgcaa gcaatggatg ttcttagaga attaggaata
    16981 aactgcaagt ttgggttcgt tttctcatcc ctggactact atcaatgaga taaagacgaa
    17041 tatcagccaa ttgaaaagta taataaaggg ggatactttt tacctttacc acccatttta
    17101 tagcgtgaat catcttgaca ctcttccgta tcccgtttag agtgttaaat atgaaacgat
    17161 ttagcttgat aattattact tttctggtac tttcctcgat gctgtgggcg gaagagcagc
    17221 caagttatgc ttataaatat ttggcttctt atccgcaaac tgcaagacaa gccatcaccg
    17281 caccactttc ctggcaaggt ggagattggc taaaagccgg agcagttgtg ctatctgccg
    17341 gagctttata tcttgccgat gaagatatcc agcattattc tcaaaatagc cgcaacgatg
    17401 gtaccgatag attgatgaat atagccagta atttcggaga atacagaatt gtggctcctg
    17461 cccttggaat cggcattttg ggtgcatatc tgcaaaatga caaaaaagca ttggacacag
    17521 gattgctctc tatgaaaagc ttcgtattat cctcggtgat atgccagggc ttgaagcttg
    17581 ccacacagcg aaaccgtcct tccgaaggcc tgggaaagaa attctggcag gaaagtaaga
    17641 taagctttga taatgacagt ttcccatctg gccattccac tgtggtttgg agtatcgcac
    17701 ccattctggc tgatcaatat caagaaagca attggattcc ccctgtaagt tatggaatcg
    17761 ccacacttac ctctctttcg cgtatataca atgatgaaca ctgggctagt gatgttctgg
    17821 cgggggcagc aattggctat ttttcagcta aactagtaag tacttccact ccccgcttga
    17881 tattagcaat aaatcccgct attcacgggg ctggattttc atataagttc taaacctggc
    17941 aaatcgcata gaatagcggg ttttgatact ggcaatatta tccacccctg taaaaggttc
    18001 ttactgctat gtagtttaat tctttttata gggttagcgc cagcttcata taggccgtgg
    18061 cgctattttt aataaaatgc cccaagggat cactgtaggt agatttctca tcctggcttt
    18121 gcgagctttt aattccggcg tagataaaat gatccctctt aaattcatat cgcatattgc
    18181 catagaactg aacatcggaa taaacgccat atagggcata attagtatag ctgattcccc
    18241 cggtgaagca taacttcaaa ttgggattgt agttcaagcg aacattacaa attgaatagc
    18301 gatcgtcaag cttaacattt gcggagccat cgccataatc tactatatta tccttgggat
    18361 agctaaaatc aatgtattgc caatcaaagg aataggaata cattttggca atattaccgg
    18421 aggcgtttgc caccaaacca aatttcccat aggtatcatt cagtacatac actaattcat
    18481 cgccatagga catccccagg gttatatgtg cccagttctc ttcagatctg gagatgctgg
    18541 agctcacccc ataatagtga tgcttttccc aaagatcatc atgcactgtg ccaaagttac
    18601 tattaaatga ataattaaag ccggacttca gatcaattcc aaaattgcca taccaagagt
    18661 actcattata ggtattgtcc ccaatgtacc aatcgctgaa atccaagctg aggaaactac
    18721 gctttttctt gatgaaacca taaccgggat ttgaattcca atccatcgag attccatagg
    18781 tctggcggtc tttatagtat agataccccg catctaaaaa gatatccttg cttaccttct
    18841 ggtagtagat ataattatca aagttcccgg ggctgaaatt caattcactg aataactgat
    18901 atcccttcag aactttatct tcctctccct cgtcattact tttaatactt cccgtaactt
    18961 ctgtattcaa catcagttcc ttgcccagct tcaaatcata attaccgaaa taagcatggt
    19021 tataataccc cttgttcatt ctgctgatta aggcattacc catagtaaaa atcgaataac
    19081 tgggaataag agacagaact tggaaataat catcattgtt ctgcacctcg ccatcttcca
    19141 ctactttttc atcttttgcc cccaatattc cccatctggt agtgccgaca ttgcccgttg
    19201 ccttgtaggc gaaagacggt ttattgatat ttcgggaata aaaaacgctt tctctaagcc
    19261 caaaggcatc cagatcctca acgaagaaaa agcgattttc ggaatacata gtaggaatat
    19321 cattattata aacatcttga gcattatccg gtggaacatc cgaaaaatcg ggattgaagc
    19381 taaatttcat cttcgtttgc tgaccgggat taagggcaat atccaagccc atcatatccg
    19441 gatcgaagct atccgtctta tttatcaaat cgtagctttt tacgaagtag ggcttaatct
    19501 tcatatccaa ttctcgtttt accttctcgt gcagaacgat tggatgcaca gccttaaaat
    19561 actccagttt gctgtctgta ttggcatagg gactgctgta atactcctta gtggactggt
    19621 gaaaacgggt aaagatcagc ccccaattat agggcaattc ttgtttaaag cgcaaggatc
    19681 ccagggggat gctaaaagta accgtccaca gcgaatcgtt atatgaggat tcataatcat
    19741 agtcgctgtc ccattgataa tctacactca tatcatcttt gcggattcca tctacgaggt
    19801 gcccggagct atatgcccca aaaaggtagg caaaattggc atccggcaaa gtaacgagct
    19861 gaaccctcag ataatctgcc tgaatctcac tatccctgct agctggatat ccaggcataa
    19921 aattctcatt aattgcgcag gaaaaatgaa ccatcagttt gccttcttcc tgccacaccc
    19981 agaccttggt agccagtgtt tcagcttggg aatcgtccgg atcaattcga acaaaatccc
    20041 cgatgagatt ggtatccgtc atcaccgcct gatcagaaaa ggggatatct attgcagaaa
    20101 gcggaaaaac gattaacaaa agcgaaaata acaggtaaca tctattcatc gggtactcct
    20161 tttgcatttt ggattttagt ttcaaataaa agctgctttt tgctgagata catattatga
    20221 cgatgcactt ttataatgcc gcagcattga tcgtgtaccc caatatgcag caataacaaa
    20281 aaccacggca cacatacttg cagctatata caattggtat ccatatcccc ctttcatcgc
    20341 catcatagga aaattaccta tcaccaatac agggattaca tagatgaaaa tccacttaag
    20401 atagcttgcc ctaaaaatag ccgccggata ggtggagagc gaaaaaaagg aagatatcaa
    20461 tgaggcaaag gcatccatgc gcacaaatat tatggaacca agatagataa gaatattgaa
    20521 tgctgtatag accattgtgg ctaatccaat cataattaaa tataaacata tactcatcac
    20581 cgagatggcg atgttatatt gagtaaatat tatcacaaat agcactacat ctattatcaa
    20641 gttcacaaag tatttccaat ttagagtact gcaaaacagc ataaagaaca aattcatggg
    20701 tttaataaga atgctatcta agctaccata ttggattagg gaaggcagag aatgaactcc
    20761 actgccaaat agcagattat acagatctcc ggttaactta accgatagca ccagtatata
    20821 taaatcctca atggagatac gcagcccttc cccaacattc aagtacaaga tttggtaaaa
    20881 aaacagagcc agcataatgc cggtcaggtc agctaaaaca tccagcaaaa agctcttatt
    20941 atagacgacc atccgcttca gttgcaagag attcgcctgt atgtaataaa caagatagtg
    21001 cttcatccaa ttgcctcata tttttttctg ccttgggacc atgctacctg agtggtcata
    21061 ataaaaacga tcaaccacat aagcgcttct aatacaatct gccaatagga cctccatgca
    21121 taatttccag tcaggatgca ggagggatgg taaacaagat atgcagaagg aagcttcagt
    21181 aggaggcccc ttattcctct gggaaaaata tcaaggggca ggatgtttcc agatgcaaat
    21241 ggcagtaata tccctagaaa attcattaca aaaccgatat ctgtgaaata gaatgccagg
    21301 atggatagat aaaaggtgat taaaaacgat aaaggtgcag cgattaatgc cataataata
    21361 aattgaatga tgctctgcaa ctttatttga ggcaccaggt tgccactaac agctatgaaa
    21421 gccagataag caacaataat ggcaaaaata gtgtataccg ctttcaaagc gtaatacacc
    21481 acaaaattat gccagaaata actgtagggc tttaatagat gatagttcaa tcctccttct
    21541 cgtatatcat tctgaatatc ccaccaggtt ccgattacga aaaattcgct tacaatagta
    21601 gctataatgt aatagagaag caactgatct aatttccaag ctccaatttg gtaacctccg
    21661 gaatatataa atttggcaag gagtagctga ccacccaggg gaatcagcac tactaaaaag
    21721 ctgaggataa aattagtccg atatgccgca gcttctttta tagtttggat gccaagcgca
    21781 gtataatacc tactcattct tcaatatctc agtgatgatc tcttcatcat cttttttctt
    21841 gatgctaaaa tcgctgatct catattgagc aaggatttgg cagattagct gctttgaatg
    21901 gttccctgtg gattcaaaga caacttcttc atcctccaca gtcgtccctc caaatttctc
    21961 tgctatttcc cgcgcaacat cattactttt tgcgctgagg gtaataacct catgctgatg
    22021 cttttccagc agaaaagtag tatcgccctg atagatgatc tcaccattat ccaaaacaat
    22081 acaggaattg caacaatcgg tgatatcctt aacattgtga ctggtgataa tgaagcagat
    22141 gccgtactct ttatgacaga aatttataaa accccttagc ttgcttcggg taatgatgtc
    22201 caatcctgaa gttggttcat ccagaaatac tattttggga agatggatga agctgtatat
    22261 cagttccatc ttgcatcttt gccccaaact catattcctt aaaggttgat ccaattcatc
    22321 gccaatatcc agagcttggc tgaaatgggt aatccgcttc ttaaattcat cgcggctgaa
    22381 gccatatata tagcgtgata attcgaggct ttggattgcg ggaagatccc atatcaattg
    22441 ttctttcccg ccctgaaaca gtcctatttg cttcttaaat tctggtttca acttatatgg
    22501 aatatgcccc agtaccaaga tttctcccga actggagtgt aatatgccgg tgagaagctt
    22561 taccagggta gttttacctg ctccattttt gccaaagata cccagattat gatccagatt
    22621 atggcaaaga gaaattcctc tgattgcctc tatttccttg taaacaggcc tgaagagcga
    22681 taccgaggtc tttacacggt attttttcca aagggactct attctgattg tccggctatt
    22741 ttccattcta atactcttgg ttagatatag gttaagtcat tcttggaata tccattatcg
    22801 tgttctccgg ttgttctttc taatcggcag ccaccataac atatagataa aaacatacac
//
LOCUS       DAOSTH010000021        21522 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_2638_length_21522_cov_14.261098, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000021 DAOSTH010000000
VERSION     DAOSTH010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 21522)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21522)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21522
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2638_length_21522_cov_14.261098"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(468..911)
                     /locus_tag="PLB65_04320"
     CDS             complement(468..911)
                     /locus_tag="PLB65_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOQ77786.1"
                     /translation="MSRTNESALQQEIMAWIKQNQYVYLATVDKNKARVRPVVLFIYD
                     DRFFVTTFTGDSKVSQIQNNKNIEVCIPLYEEGQTGYIRLSGTASIVHNATLKAEASE
                     RCFFFDDYFSGYDDPDYTLIEFDFEYAEYLRPGESYSQGCNLKRY"
     gene            complement(924..1784)
                     /locus_tag="PLB65_04325"
     CDS             complement(924..1784)
                     /locus_tag="PLB65_04325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ77787.1"
                     /translation="MRNRNKTSIPILIVLMVISISIGCQTKSSPIETSKEPLMSQKEN
                     TTTTTKVEMETTYGTIELELWNDLTPKTVQNFIKLASSGYYDGLYFHRVIPEFMIQGG
                     CPNTRDKDRSNDGRGGPGYTFEDECYKNGNEITGKIDTNQKAEAVWTKIIVPHIQTNR
                     TPNPDIATIAKECQEVRNFDPIMKHDLQFYQEKTGIKDKIYIQELIAPVNYGTICMAN
                     SGANTNGSQFFIVTKKDGAHWLDGKHTVFGKVTKGMEVVHKIENLPRDKNDNPNPENQ
                     AFITRISFPK"
     gene            complement(1801..2550)
                     /locus_tag="PLB65_04330"
     CDS             complement(1801..2550)
                     /locus_tag="PLB65_04330"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOQ77788.1"
                     /translation="MKIIALSDSHYKFHCTDKADRENASIFVSFLNQCLGKYDLMVLS
                     GDIFDFWLEGKYTIVKQYFPVLCALNAIKEKGTQIIYISGNHDFWFGDFLSEYIGCKI
                     YPDGFTLNTDGKKIRFEHGDTRTLNDLRYQIYRKIIRWKVVRKIAASLHPDLALNIGM
                     LFSRSSREHTDNPALVERKSFGLKNYAKRLIEHNRADIVVLGHSHKPALEKIGNGYYV
                     NCGDWIKHYSYVEIDAGNPTLHKYNKESNQS"
     gene            complement(2547..3215)
                     /locus_tag="PLB65_04335"
     CDS             complement(2547..3215)
                     /locus_tag="PLB65_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HOQ77789.1"
                     /translation="MHRYFIILGLVICLPLLAVVETPIKTGFARLQYDGGGDWYNDPE
                     VLPNLAKYANLTMGTSFPLDQSVVKASDPKLFDFPFLYLTGHGNIKFSEREVENLRTW
                     MLRGGFLYADDDYGMDESFRREIKRIFPDRELMELDPSFPLFNCFFDFSSGLPKIHLH
                     DGKPPQAYGVFDDNGRLLCLYTYETNISDGWADPETHNDPPEIRDKALKFGVNILYYV
                     INKK"
     gene            complement(3219..4628)
                     /locus_tag="PLB65_04340"
     CDS             complement(3219..4628)
                     /locus_tag="PLB65_04340"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HOQ77790.1"
                     /translation="MISLHSCEASPPRSVCEYKASRDGSFAWFLLTFHPEKDNVLEEI
                     VAEIKLEVINSKINAISRLEIEKWLKQFFSDLHWKLYGILRKTTLNEKGISLFFGVLY
                     DDELYFVQFGRIFCVLADHKKMKPVGKDWRNYQVQSLEKMQLLGYNEKDLIIKPQRVR
                     IENNQLFVVISGELAQKIFLTVTDPVSIETLIETYITEDNPLWLILGGSENLAKPKRR
                     KLSRLQVTSIILIFLAVLAAVYVMVGNRFLDQMVHKMRLSVKEEKPLRLEQIPTNLNI
                     NSENLKKYMDKLVNMPARNISFTVAWTTELPYQVSLSPSFTLSTIYLVSGNRLSAYDK
                     KSRQLLWTKILEDEILSTTVHQGLLMIYLANQQILGLNTDGIVLWQQYLPTESITGDR
                     FVPCIISNSDDPRLDRSIMVIPSKRGISILDPAKGETLSSITLKQDLDALSVYDSFAN
                     CFYAVVDNAVLCVELNIVN"
     gene            complement(4630..5712)
                     /locus_tag="PLB65_04345"
     CDS             complement(4630..5712)
                     /locus_tag="PLB65_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ77791.1"
                     /translation="MIYLIFRIMGYWLMKNLGFPHLMPMNYTVSLLYTCNSRCSTCNI
                     WKKQANNLSVDEYKKIFKHIKHTPYWITFSGGEPFLRNDIVEVVTSIYQISRPRIINI
                     PTNGLLVKTIIEKTDAIAKACPKAQIIINVSVDGIDEQHDNIRNVPGNYKKAIATFNG
                     LKALKHKNLSVGIHTVISRFNVDSFPSIANELMRLKPDSYITEIAEERVELDTIGTDI
                     TPSLVAYKSAIDYLIHRIKNTTYKGMSKVTMAFRIEYYNLVKRIMRDKRQIIPCYSGV
                     ASCQISPEGDVWNCCVKAKPLGNLREAKYNFRKIWFSPAAELERRSIHKRECWCPLAN
                     AAYTNMLLHLPTLIRVSWRSFIRWWC"
     gene            complement(5715..6623)
                     /locus_tag="PLB65_04350"
     CDS             complement(5715..6623)
                     /locus_tag="PLB65_04350"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ77792.1"
                     /translation="MNKIKCSVGIFAHNEAANIIPLLQAVENQELQETEISEIIVVSS
                     ASTDGTDTLVSSYAKDHNKVTLFTQPKREGKSSAINLFLANAKEDIVVIISADVIPQK
                     KTIEKMVSAFKDPKIGATGGRPVPVNDENTYLGYVVHLLWRLHHRMALISPKLGEMIA
                     FRKVMKSIPKDSAVDEASIEAIIKEQGYKLKYIPSAIIKNKGSENLSDHIKQRRRIQN
                     GHLWLKAHQHYKVSSQDSGILLKVVLQELIERPGSFFKLALAMLIEIYCRLLGSWDYY
                     IKGKNPFAWEIARSTKELQRPIKDKE"
     gene            complement(6627..7094)
                     /gene="nusB"
                     /locus_tag="PLB65_04355"
     CDS             complement(6627..7094)
                     /gene="nusB"
                     /locus_tag="PLB65_04355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOQ77793.1"
                     /translation="MGQRRKAREMAVQCLYSLEFSEVDKEYREYGLLNEYPEILLSLA
                     KQEHIQPNSPVCAFAEELVKNSIINIEQIEAVLDKLTDNWDLEDIALLDRSILCISIY
                     ELLYTDTPAPVVINEAIEIAKKFSSESSGKFINGILDALNKEIIQNQKGNLPI"
     gene            complement(7097..7558)
                     /gene="ribE"
                     /locus_tag="PLB65_04360"
     CDS             complement(7097..7558)
                     /gene="ribE"
                     /locus_tag="PLB65_04360"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOQ77794.1"
                     /translation="MKVIEGKLISRGYKFALVVSRFNEFISSRLLEGALDCLKRHNVN
                     DEDISVIWVPGAFEIPLIAKEAALKEDIDAVICLGAVIRGSTPHFEYISAEVTKGIAT
                     VGLETRKPIIYGVLTTDSIEQAIERAGTKAGNKGFTAASSALEMLNLIAEM"
     gene            8050..8715
                     /locus_tag="PLB65_04365"
     CDS             8050..8715
                     /locus_tag="PLB65_04365"
                     /inference="COORDINATES: protein motif:HMM:NF027589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HOQ77795.1"
                     /translation="MIAVLTCDIISSRSYDKKQRQILQTSISDAFSYTCKIIPEAKAD
                     YMSFSVIQGDEFQFNIEEAPYFYHFLFTFRNQLSLCGIEPPPFFRAGIGFGTRTIEGK
                     NNSYQMDGTAYHNSRFALNSFSENAYKKRLSIVRLDNPLLTKCYNTVLAFCDDWESSI
                     SFPQRRAIQQILTGLTQRQAAQNLGFTEQNMVKLLKRAKWDLFSQAMDFFCTTLPENL
                     PTQ"
     gene            8739..8930
                     /locus_tag="PLB65_04370"
     CDS             8739..8930
                     /locus_tag="PLB65_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77796.1"
                     /translation="MKTANLSVIEHICLAVYVSTIYGLVGINKPNTVVSLKVNWNWLM
                     LFNVSFSLSPNKSSLLEGE"
     gene            8930..9670
                     /locus_tag="PLB65_04375"
     CDS             8930..9670
                     /locus_tag="PLB65_04375"
                     /inference="COORDINATES: protein motif:HMM:NF023178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HOQ77797.1"
                     /translation="MLLWRLLLALFISDFLLQNGRLIKAKKNFTGLIIHCAIYFVVML
                     CLTANLLTGKIIVVLLILAILHGIVDYTKQILRPIFANREWLLFITDQFIHILTIILA
                     VGIISDLDCSYLIAIYNKFPIDAWFKYLALFIVTVFAGIYFTDSALQGFLKHFTKPDN
                     TKQKPNAKQQEYASRWIGVSERFLITISVLIGRYELIGFLIAAKSLIRLPEIQDEKTE
                     DFSNYFLVGTLLSYSWAIAITLLFKKLF"
     gene            10099..10665
                     /locus_tag="PLB65_04380"
     CDS             10099..10665
                     /locus_tag="PLB65_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77798.1"
                     /translation="MWLPVTNAIKLNFGKKIKANCISCSAKIIILSFFIFSLGPTIIF
                     AQSNSEEYLEVKNWKAPKDAVAFTELTVQDSIYLYKDKPFTGVAVEHYPNGNLLRAVT
                     YIRGKQSGPMFLWYPDGNPQMSAYYQNGYLHGRFLGWYSNGSVIYDIVINKGTYAGDN
                     LLDEDEGRIGLEMEEGEREGPDNDQSKE"
     gene            10646..11470
                     /gene="panB"
                     /locus_tag="PLB65_04385"
     CDS             10646..11470
                     /gene="panB"
                     /locus_tag="PLB65_04385"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOQ77799.1"
                     /translation="MTKVKNKKNGIALFQDMKKCKEKISMITAYDYSMAKCVSATSID
                     IILIGDSLGMVVLGYEDTLQVTVENVIYHCSAVRRGAPETFIIADMPYLSYHLSEEQT
                     RKNAADLIIKGKADAVKLEGGYPSRIDAIKAIVDMEIPVCAHLGLTPQSIHKFGGYKV
                     QGKKPEQQEELLKQALAVEKAGAFMLVLEGIPEILGKEITEQINIPTIGIGAGRYTDG
                     QVLVYNDLLGYSDMQPKFVKHYAEINSNIKEAIELYSQEVKNGVFPAPEHIYYPIT"
     gene            11497..12117
                     /locus_tag="PLB65_04390"
     CDS             11497..12117
                     /locus_tag="PLB65_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77800.1"
                     /translation="MPNKIMPPESMSAEEKFAALANLKDKLEDNFVMLGQLLSEIKRS
                     KLYLFKGYENFKDFVETEYQLSGSLAGKLISTFDLFIEEMDIDENEVKEIGFDRLQMI
                     KPFMKKADWQVRDEWIHKAEEMPFQELRDHIKELKEKEKETNVDLKEVYIEQFMEKML
                     GWFNCSRKELNFKLALFFQDADLDQMKKIVKERQRLFELETQTNKE"
     gene            12124..13692
                     /locus_tag="PLB65_04395"
     CDS             12124..13692
                     /locus_tag="PLB65_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ77801.1"
                     /translation="MKQMHILWVDDEIDLLKPFILFLEERNYLVDTCNNGTDAIEKVV
                     ENKFDLVILDEMMPGLDGLATLQEIKRINPALPIVMVTKSEEEGLMDKAIASQISDYL
                     IKPINPNQIIMAIKKIFQADEIRANQIGQQYTRYIAELNQKLFATPDWDEWSQIYREL
                     SYWELQIDQVNDEGLRQTHFLEKRNCNTEFTNYVERNYNKWLKSDERPNLSFDLISQH
                     IAPHFEENLPIYFIIIDCMRLDQYLAIQPYIQELFDEELDLYYSVLPTATPYSRNSIF
                     SGLLPIDIAKRFPDYWVTSSEMDNSRNRNEHQLLDEHIEELGYKLDPTSKYVKIFNME
                     EGNFVLRKIETWNKENLIVLVYNFLDLLAHHRSRDQILQETIPNEEGLRAFTKHWFLH
                     SSLYEALKLIAKQDAIVILSTDHGSIKVNRATQVIGDKDTSVTVRYKEGKNLSVNDRH
                     ALFVKKPSDYGLPAKSIVDNFIFAKDDYYFVYPNAYHQYQRQYNGTFQHGGVSMEEMI
                     LPIATCRTKKRTKE"
     gene            13726..14163
                     /gene="tsaE"
                     /locus_tag="PLB65_04400"
     CDS             13726..14163
                     /gene="tsaE"
                     /locus_tag="PLB65_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOQ77802.1"
                     /translation="MITINLPSEQDTIDLANYLAPILKAGDIITLYGDLGSGKTFFVK
                     HLGKELGINEEIDSPSFIIMKEYLGGRLPLYHLDLYRLKDKEELYDLGIFDILEKGVT
                     VIEWPHIAESILPYQTFKLDFHFDGKSRWVNVFPDDDHKQYFE"
     gene            14176..14958
                     /locus_tag="PLB65_04405"
     CDS             14176..14958
                     /locus_tag="PLB65_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425332.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOQ77803.1"
                     /translation="MFTVLLVLICIAIALCLGWIIFAKDKAISRTWGIVGLSFVIGLT
                     VFGYFFMQSRQAPTSLRVVGYASKLFESDIVKWNLTMQRNVSNDGLKDGYNKMSADVN
                     AFKKYLLEQGIPEKDISIQPTNSYQTNDNYGNPTGYCLNQHIFVVSTDIDKIEKLSLN
                     PDFFAERGILLQNSNLAYLYTKLPDLKKQLLSEATKDAVARAEEISGSGNTKLGKLRE
                     ARAGVFQITEPYSTEVSDYGFYNTSTRSKSISVTLTAIFKLK"
     gene            15063..15812
                     /locus_tag="PLB65_04410"
     CDS             15063..15812
                     /locus_tag="PLB65_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOQ77804.1"
                     /translation="MNKNLFVLTLLLTIILLVASCSPEQKKILRVGTDAENPPFTYLE
                     NSEFKGIDIDICEKIADKMGMQLQITQYEFDDLFSALAQNKIDLAASTITITEKRKQT
                     FEFSEPYFQTNLAVVARKASPIEINKIEDIGKYRVGCQSSTTSYQYLKENLVDKDLLP
                     KEKLKTYSTLIETIGELLNKKIDLMVFDEWVAKKVSEQVDIKIVYIIPTNEQYGLAMQ
                     PGKEINLRINKALKELKDSGELQRIINEYIK"
     gene            complement(15885..18335)
                     /locus_tag="PLB65_04415"
     CDS             complement(15885..18335)
                     /locus_tag="PLB65_04415"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ77805.1"
                     /translation="MILTDAQLFSEISRCEYCENKPCKAACPCDCSPADFMMALAKGR
                     PSDFKRAAGIILASNPLGGICGNVCPDYHCVDACSHKKFDKPIQIPDVQATIIRKARE
                     LDLVPEFSSPIRLNTKIAIVGAGPSAIGAAVTLNQFGYETRMFDPEPLGGQINLISEE
                     RLEEIDIIADLNFVVKTFRLQHIPKKIEKPEELLKEGYAAVIVASGLNVPIRLNIPGD
                     ETAIYGLDILSNPSKYNFSGKRIAIVGGAIAADIALVAAKNSAAYVEMITLENFVEMP
                     ITDREKATLIEAGIEFSPRTRISEIVNENGHLKGIKTKKVYLPKNEKFAPNLIKDEAG
                     VIDNYRIFDMVIIAIGNRATFNVHELERKFDNIFCCGDMVNGPTTVVEAVASGKNTAI
                     KVHLSLSNKAIEKPEKETKSCFPVWGWKKYPVSLETDFFGRIMDSPFILSAAPPTDGF
                     EQMQKAYQAGWSGGIMKTAFDDQDIHIPGEYMFTFGDKKKTFANCDNVSEHSLNRVCK
                     EIARLIKEFPDRLTMASTGGPVTGNDCSDKQCWQSNTKKLEQAGAMGIEFSLSCPQGG
                     DGTKGDIVAQDPVLTAKIVDWVMEISAPEIPKLFKLTGAVTSIYPILSAIKNVLNKYP
                     HKKAGITLANTFPALAFREKEKPWDEGIIVGMSGEGVLPISYLNLARAGNQGIFISGN
                     GGAMNYLDAANFLALGARNVQFCTIVEKYGYNIIEELKMGLSYLLEARGLKSVAELVG
                     IAQPNPIIDFMNLPKKIKSSSLNRELCLKCGNCTRCPYLAIELDEDCFPKIDNDQCVG
                     CSLCTQLCFTGALQMKKI"
     gene            complement(18563..20098)
                     /locus_tag="PLB65_04420"
     CDS             complement(18563..20098)
                     /locus_tag="PLB65_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOQ77806.1"
                     /translation="MLKVSIAPDGKEYPLPIEEDAEKERETLKRITAEQRSLGRKIVA
                     VQGLGFVGCVMATVVADATDKDGNPIYFVHGHQRASQRSYWKVPVINNGVPPINSSDP
                     EVPEIFHRTVVEKKNFRATSEESVYSLVDIVVVDIQLDATKPAFGDAEKGYCDITAFR
                     EGIRTLGKYIQPECLVLVETTVPPGTCEQVVKPILEEEFTKRGIDINNQPPLIAHSYE
                     RVMPGAHYVESIRNFWRVYSGVNTKSIELCREFLSNVLDVEHFPLTQLDSTNASELAK
                     TMENSYRAVNIALTLEWAKFAEQIGVDIFKVREAIRKRKGTHDNLLRPSLGVGGYCLT
                     KDPVLANWAMNSLFGVEGTLDFAIKAVNTNDTMPLHTIDIVKSVYPELKNLKIAVLGV
                     SYLQDVGDTRHSPSKILVEFLRKDLAIVKTHDPYVESWPEMEENHIQSDLNNVLPESD
                     VVIFAVGHSQYRNLDPEQLLAICKTKPLIVDCSNFLSDETINKYKALGCAVRGVGKGH
                     IID"
BASE COUNT         6860 a         3963 c         3714 g         6985 t
ORIGIN      
        1 taggcattct ttttatcgcc gggttaaaac ccgtcgttag tatgtatcgt tcctaacgga
       61 acttcaaatt agaaatcctc ttaatcctaa aatcctaaaa tcctcgtttc gaaaatctat
      121 tcctaaaatc cttgtttcta aagttttaat ctgtcgtccc gctgggactt cttttaggca
      181 ttctttttat cgccgggtta aaacccgtcg ttagtatgta tcgttcctaa cggaacttta
      241 tagaagaaat cctcttaatc ctaaaatcct aaaatcctcg tttcgaaaat ctattcctaa
      301 aatccttgtt tctaaaactc aaatctgtgt aaattatttt cggtgacttc ggtggcctcg
      361 gtggctgaat attcttctac aatcctttca gcctcggaac ctccagatat ctatatcttc
      421 accagatatt gtgaatatgg tattttgtct tatcttatgt atgttattta gtatcttttc
      481 aagttgcaac cctggctgta gctttcaccg ggtcttaagt attctgcata ttcaaaatcg
      541 aattctatta gagtataatc gggatcatca tagccagaaa aatagtcatc aaaaaagaag
      601 caacgctcag atgcttcagc tttgagcgtt gcattatgaa caatagaggc tgttccgctt
      661 aagcgaatat aaccggtttg tccttcttca taaagaggaa tgcagacctc tatattttta
      721 ttattttgaa tttggctaac cttagaatcc ccggtaaaag tagtaacaaa aaatcggtcg
      781 tcatagataa agaggacaac gggtctaact ctggctttat ttttatctac agtagccaga
      841 tatacatatt ggttttgttt tatccaagcc attatttctt gttgcagagc ggattcattg
      901 gtgcgtgaca taattatctc attttattta gggaaggata ttcgggttat aaatgcctga
      961 ttttccgggt ttggattatc gtttttatcc cggggtaaat tttctatttt atgaactact
     1021 tccatacctt tagtcacttt tccgaaaacg gtatgtttgc cgtccagcca gtgagctcca
     1081 tcttttttag taacgataaa gaattggctg ccattagtat tggctccaga atttgccata
     1141 cagattgttc cgtaattaac gggagcgatt aattcctgaa tgtatatttt atctttaatt
     1201 ccggtttttt cctggtagaa ttgaaggtcg tgtttcataa taggatcgaa gttacgaact
     1261 tcctgacatt ctttagcgat agtagcaata tcaggattag gagtacggtt agtttgaata
     1321 tggggaacga taattttggt ccagacagct tctgcttttt ggttggtatc aattttgcca
     1381 gtaatttcat tcccgttttt atagcattca tcttcgaaag tatatccagg accgccgcgt
     1441 ccatcgttgc tacgatcttt atctctggta ttagggcatc caccttgaat cataaattcc
     1501 ggaataacgc gatggaaata taaaccatca taataaccgg aagaggcaag tttgatgaaa
     1561 ttttggacag ttttgggggt aagatcgttc caaagttcca gttcaattgt tccgtaagtg
     1621 gtttccattt ccacttttgt agttgtagtg gtattttctt tttgactcat taatggctcc
     1681 tttgatgttt ctataggcga agattttgtt tggcatccga tactgataga gatcaccatt
     1741 aaaactatca gaatcgggat agaggtcttg tttcgatttc gcatattata ctccttttgg
     1801 ttatgattga ttgctttctt tattatactt atgcaaggtt ggatttcctg catcaatttc
     1861 cacataagaa tagtgcttta tccaatcacc gcaatttaca taataaccat ttcctatttt
     1921 ttccaaagca ggtttatgac tatgtcccaa tactactata tcggcgcggt tgtgttcaat
     1981 taacctttta gcataatttt tcaatccaaa gcttttgcgt tcaacaagag caggattatc
     2041 tgtatgttct ctgctacttc tggaaaagag cattcctata tttaaagcca aatcggggtg
     2101 taatgatgca gcaattttac ggactacttt ccagcggata atttttcgat atatctgata
     2161 gcgcagatca tttaaagtgc gtgtgtcgcc atgctcaaaa cgaatttttt taccatctgt
     2221 atttaaagta aagccatcgg gatatatctt gcagccaata tattcagata aaaaatctcc
     2281 aaaccagaaa tcgtgatttc cacttatata aataatctgt gttccctttt ctttaattgc
     2341 atttagagcg catagaacag gaaaatactg ttttacaata gtatatttgc cttccaacca
     2401 aaaatcgaaa atatctccag aaagcaccat taaatcgtat ttacccagac attgattcaa
     2461 gaagcttacg aaaattgagg cattttccct gtctgcctta tctgtgcagt gaaacttata
     2521 atgactatcg gatagcgcta ttatcttcat tttttattta tcacatagta aagaatattt
     2581 actccgaatt taagtgcttt atctcttatt tcgggagggt cgttatgcgt ttcaggatct
     2641 gcccaaccat cactgatatt agtttcgtaa gtatataaac aaagcaggcg tccattatca
     2701 tcaaaaaccc cgtatgcttg aggaggttta ccatcgtgta aatgaatttt gggaaggcca
     2761 ctactgaaat cgaaaaagca gttaaaaagt ggaaaagaag gatctaattc catcagttct
     2821 ctatctggga aaatgcgttt tatttctctt ctaaaagatt catccattcc gtaatcatca
     2881 tcagcatata aaaaaccacc tctcagcatc caggttctaa ggttttctac ttcgcgttcg
     2941 ctgaatttga tgtttccatg gcctgttaaa tacaaaaaag gaaaatcaaa aagcttagga
     3001 tcggatgctt tcactacgct ttgatcgaga ggaaaacttg tccccatagt cagattggca
     3061 tattttgcca gattaggtaa aacttccgga tcgttatacc aatcgcctcc tccatcatat
     3121 tgtaaacggg caaaacctgt ttttatagga gtttctacta ctgctaaaag aggtaggcaa
     3181 attactaatc ccagaataat aaaatagcga tgcatagctt aattcacaat gtttagctct
     3241 acacacaaaa cagcattgtc tacaacagca taaaaacaat tagcaaagct atcataaacg
     3301 gatagagcat ctaaatcctg ttttaaagta atactactta gtgtttcacc cttggctgga
     3361 tcaagaatgc tgattcctct tttcgaagga ataaccataa tgcttctatc cagacgggga
     3421 tcatcactat tgctaattat acaaggaaca aaacgatctc cggtgatact ttctgttggc
     3481 agatattgtt gccagagaac aattccatcc gtattaagcc ccaaaatttg ttgatttgcc
     3541 agataaatca tcaacaatcc ctgatgaact gtagtggaga ggatttcatc ttccagtatt
     3601 tttgtccaga gcagttgacg agatttttta tcataagcac tgaggcgatt tccggaaaca
     3661 agataaatag tgctaagtgt aaaagaagga gataaactca cttgataagg cagttccgta
     3721 gtccaagcaa cagtaaaaga aatatttctg gctggcatat tcaccaattt atccatatat
     3781 ttcttcaaat tttcactatt gatattcagg ttagtgggaa tttgttctaa ccgtagtggc
     3841 ttttcctctt ttacagaaag gcgcatttta tgtaccatct gatcaagaaa acgattgcct
     3901 accataacat acacagcagc cagtacagct aaaaaaatta agataatgct ggttacctgc
     3961 aagcgagaaa gttttctgcg tttaggcttt gccaaatttt cgctaccccc caaaatcaac
     4021 cagaggggat tatcttctgt gatatatgtt tctattaaag tttctataga tacaggatct
     4081 gtaacagtga gaaaaatctt ttgtgccagt tcaccggaaa taactacaaa aagttgatta
     4141 ttttcgatac ggactctttg gggtttgata ataaggtctt tttcattata accaagcagc
     4201 tgcattttct ccaaggactg aacttggtaa tttctccagt ccttacccac aggtttcatc
     4261 tttttgtgat cagctaaaac gcaaaaaatc ctgccaaatt ggacaaaata aagctcatca
     4321 tcatagagca caccaaaaaa gagagaaata cctttttcgt tcagtgtcgt tttgcgtaaa
     4381 attccatata gtttccaatg cagatctgaa aaaaattgtt ttagccactt ttcaatttcc
     4441 aaccgagaga tagcgttaat tttagaatta atcacctcca gcttaatttc ggcaacaatt
     4501 tcttccagca cattatcttt ttccggatgg aaggttaaca aaaaccaggc aaaacttccg
     4561 tcccttgatg ctttatattc acatacactt cggggaggag atgcttcgca gctatgtaaa
     4621 gatatcattt caacaccacc agcgaataaa actacgccag gaaacgcgaa ttaaagttgg
     4681 aagatgaaga agcatatttg tgtaagcagc attagctaaa gggcaccagc attctctttt
     4741 atgaattgag cgtcgttcta attctgcagc aggagaaaac cagattttac gaaaattata
     4801 tttagcttca cgcaagtttc ccaatggctt agctttaacg caacaattcc aaacatcgcc
     4861 ctcgggagat atctggcaag aggcaactcc tgaataacag ggaataattt gtctcttatc
     4921 cctcataatt cttttcacca ggttgtaata ttcaattcgg aaagccattg ttaccttgct
     4981 cattccttta taagtggtat tcttaattct atggataaga taatcaatag cacttttata
     5041 ggcaaccaga gaaggtgtaa tatcggttcc tattgtatca agttccactc tttcttctgc
     5101 aatttcagta atatagctat ctggtttcaa acgcatcagt tcattggcta tggaaggaaa
     5161 gctatctaca ttaaatcggg aaatgacagt atgaatccca acactgaggt tcttatgttt
     5221 cagcgccttt aaaccattaa atgtggcgat agctttttta tagtttccag gaacattacg
     5281 aatgttatca tgctgctcat caattccatc gacagataca ttaattatga tctgagcttt
     5341 aggacaagcc ttagcaatag catcagtttt ttcgattata gttttaacca gcaaaccgtt
     5401 agttggaata tttatgattc ggggacgaga aatttgatag atagaggtaa ccacttctac
     5461 aatatcatta cgcagaaaag gttcacctcc gctaaaagta atccagtaag gggtatgctt
     5521 aatatgttta aagatcttct tatattcatc aacactaaga ttatttgcct gttttttcca
     5581 gatattacaa gtgctgcaac gagagttaca tgtatacagt aaactaacag tataattcat
     5641 tggcatcaga tgaggaaaac ccaagttttt catcaaccaa tatcccataa ttctaaagat
     5701 gagataaatc atatttattc tttatcctta attggtcttt gcaattcttt tgtagaacgg
     5761 gcaatttccc aagcaaaagg atttttaccc tttatataat aatcccagct gcccagaaga
     5821 cgacaatata tttctataag catagctaaa gcaagcttaa aaaaacttcc aggccgctct
     5881 atcaattcct gtaacactac tttcagaagg attccactat cctgagaaga aaccttgtaa
     5941 tgctgatgcg cctttaacca taaatgacca ttttgaattc ttcttcgttg ctttatatgg
     6001 tcagataaat tctctgatcc cttattctta ataatagcgg aaggaatata tttaagttta
     6061 tacccttgtt ctttgatgat ggcttcaatg ctggcttcat caacagcact gtctttgggg
     6121 atgcttttca tcacctttcg gaaagcgatc atttctccca atttaggaga tatcaaagcc
     6181 atccgatgat gtaaacgcca taaaagatga acaacataac caagataagt gttttcatcg
     6241 tttactggaa caggcctgcc tcctgtagcc ccaatttttg gatctttaaa ggcagatacc
     6301 attttttcta tcgtcttctt ctgtggaata acatcagcgc tgatgattac cacaatatct
     6361 tctttggcat tagccaaaaa aagattaata gcggaagatt taccttcgcg ctttggctga
     6421 gtgaagagag ttactttatt atggtctttg gcataagaac taaccaaagt gtcagttcca
     6481 tcagtactgg cactggaaac aactattatt tcgctaattt ctgtttcctg caattcctga
     6541 ttttctactg cttgtaacag aggaatgata ttagctgctt cattatgagc aaaaattccc
     6601 acggagcatt tgatcttatt catagcttaa attggtaaat tccctttctg gttctgaata
     6661 atttctttat taagggcgtc cagaatgccg ttaataaatt tgcctgagga ttcactgctg
     6721 aattttttgg caatttcaat cgcttcatta ataacaaccg gagcaggtgt atcggtatat
     6781 aatagttcat atatagaaat gcataaaata cttctatcca gcaaggcaat atcttctaaa
     6841 tcccaattat cggttagttt atcgagtact gcttctattt gttcaatatt gatgatggag
     6901 ttttttacca gctcctcagc aaaagcacaa acaggagaat tgggttgaat atgttcttgc
     6961 tttgccaagg aaagcaaaat ttcgggatat tcatttagca gaccatattc acgatattct
     7021 ttatccactt ctgaaaattc cagagaataa agacattgaa ctgccatttc acgagcttta
     7081 cgacgttgcc ccatttttac atttcagcta taagatttag catttccaaa gcagaactgg
     7141 cggctgtaaa acctttatta ccagctttag ttccagcacg ctcaattgcc tgctcaatac
     7201 tatctgtagt taacacccca taaataatag gtttacgggt ttctaaccct acagtagcta
     7261 tgcctttcgt aacttctgca ctgatatatt caaaatgggg agtggaacct ctaattacgg
     7321 ctcctaaaca gattaccgca tctatatctt cttttaaagc tgcttcttta gcaattaaag
     7381 gtatttcaaa agctcccgga acccatatta cactaatatc ttcatcgttt acattatgac
     7441 gcttcaaaca gtccaatgct ccttccagta aacgagaact gatgaattca ttaaaacgac
     7501 tgactaccag agcgaattta tatcctctgg agattaattt tccttcaata actttcatct
     7561 tttttttctc cttgcgtatc cggagctttc cataaactcc gcattctgta gaatattttc
     7621 ttccttatct ttaggggcgg taatatctct aatatttata cctcttttgt actcccaaag
     7681 gtaatatatc ggctcactat aataataggg tatatattat tcaaaggata gaaaactttt
     7741 gctatattag cacttagaga agtgaagttt tcccactttg ctatattaac tcctgttatc
     7801 ttgtctattt ttatgctaaa taccattatt taaattattg gaatttgttc aaggaaaatc
     7861 tttcatattt cggcgctacc tatattttct gtataaatca ttttttcata accagtagca
     7921 ttcaaccaaa ggaaaaattg aatcctcggg aactaaaaag aaaattccat cttgcttcag
     7981 ctttttgctt gacacttatc aaatccaata atataatgtt aataacataa aaatcgattt
     8041 gaggaaataa tgatagctgt tctaacttgt gatattatca gctctcgatc ttacgataaa
     8101 aagcaacgac aaatattaca gaccagtatt tctgatgctt tctcttatac ctgcaaaatc
     8161 attccagaag ctaaggctga ctatatgtca ttttcagtta ttcaggggga tgaatttcaa
     8221 ttcaatattg aagaagcgcc ctatttctat cattttttgt ttacttttag aaatcaacta
     8281 tctttatgtg gaattgaacc acctcctttt ttccgagctg gaatcggttt tggaaccaga
     8341 accattgaag ggaaaaacaa cagctatcaa atggatggaa ctgcatatca taattcccgt
     8401 tttgctctaa atagcttttc tgaaaacgct tataaaaaga ggttgagcat tgtaaggtta
     8461 gataaccctc tactaactaa atgttataac accgttcttg ccttttgtga tgattgggaa
     8521 agttccatta gttttccaca acgcagagca attcaacaaa tccttacagg tcttactcaa
     8581 cgccaggcag ctcaaaattt gggatttaca gaacagaata tggttaaact tttaaaacgc
     8641 gcaaagtggg atttgttttc tcaggcgatg gatttttttt gcacaactct tcctgaaaat
     8701 ttaccgactc agtgatgttg gaaaatacta taagcacaat gaaaacggca aatttatcag
     8761 taatagagca tatttgttta gctgtttatg tctcaaccat atatggttta gtaggtataa
     8821 ataaaccaaa tacagttgta agtttgaaag ttaattggaa ctggttgatg ttattcaatg
     8881 tcagcttttc cttatctcct aataaaagtt cattactgga aggtgaataa tgctattatg
     8941 gcgtttattg ttagctttgt ttattagtga ttttttactg caaaatggtc ggttgattaa
     9001 agcgaaaaag aattttacgg gtttaattat tcactgtgca atctactttg tcgttatgtt
     9061 atgcctgaca gccaatttgt taacaggtaa aatcattgta gttttgttga ttttagccat
     9121 tctccacggg attgtggact atacgaaaca gatattacgc cctatttttg ccaatagaga
     9181 atggctactg ttcataactg atcaatttat tcatatatta accatcattt tagcggtggg
     9241 gataatttcc gatttagatt gcagttatct aattgccatt tataataaat ttcctattga
     9301 tgcctggttc aaatatttag ctttattcat tgttactgtt tttgccggta tttacttcac
     9361 ggatagcgcc ctgcaaggtt ttttaaaaca ttttaccaaa cccgataata caaagcagaa
     9421 accaaatgca aaacaacaag aatatgcgtc gcgttggatt ggggtatcag aaaggtttct
     9481 cattactatc tctgtattga ttggtcgtta tgaattaatt ggttttttga ttgctgctaa
     9541 gtccttaatt cggcttcctg aaattcaaga tgaaaaaact gaggatttca gtaattattt
     9601 tctggttggc acgctactaa gctattcttg ggcaatagct atcacgctgt tattcaagaa
     9661 gttattttaa caaacaatcc tatagggaaa ctgttttaat ccttttaaat aattattggc
     9721 aggtttgttc ttgcttaatt atctacggta gtgctggaat ggtgttacag aggaatttgc
     9781 cgagcatttc caccgcagtt ctaccacagg tgactaagca aaaactaggt ttattttatt
     9841 aattgaagag gagattcatt tcttttctta gagcataact cgagataaat tcaatggagg
     9901 aaaaaggatt atacatagat agagatagtt atggccttac tattaggtaa tgctgttccc
     9961 cgaccttata taaatttagc tttattatgg cttgataatt ctttgttttg aaaagacaaa
    10021 gtttagatta tcatatctaa tgaacggggt ttctcgcaat aaataaaaaa agattgacta
    10081 accctttgga gtaaaaaaat gtggcttcca gtgactaatg ctataaaatt gaatttcggt
    10141 aaaaagatta aagctaactg tataagctgt tcagcgaaaa taattatact atcctttttt
    10201 atcttttccc ttggccctac gattatcttt gcccaaagta atagcgagga atatttagag
    10261 gtaaaaaact ggaaagcgcc taaagatgcg gttgctttta cagaacttac ggtgcaggat
    10321 agtatttatt tatacaaaga taaacccttt accggagttg ccgttgaaca ttatcctaac
    10381 ggtaatttgc ttagagctgt aacctatatt agaggaaagc aaagtggccc tatgtttctt
    10441 tggtatcctg atggtaatcc tcaaatgagt gcatattacc aaaatggata tttacacgga
    10501 cgctttttag gctggtacag caatggtagc gttatttatg atatagtaat taataaagga
    10561 acttatgctg gcgataactt attggacgaa gatgagggcc ggataggttt agaaatggaa
    10621 gaaggagaaa gagaaggacc cgataatgac caaagtaaag aataaaaaga atggaattgc
    10681 tcttttccaa gatatgaaaa agtgcaaaga aaagatcagt atgattactg catacgacta
    10741 ttctatggca aaatgtgtgt ctgcaacatc tatagatatt atcctcattg gtgattcttt
    10801 gggaatggtt gttctgggct atgaggatac tctgcaagtg actgtagaaa atgtaattta
    10861 tcattgttca gcggttcgta gaggagctcc ggaaactttt attattgctg atatgcccta
    10921 tctaagctat catctttcag aagagcaaac ccggaaaaat gctgccgacc ttataattaa
    10981 aggaaaggcg gatgccgtaa aactggaagg tggctatcct tctcgtatag atgctataaa
    11041 agctattgtg gatatggaaa tacctgtgtg tgcgcattta ggtttaaccc cccaatccat
    11101 tcataaattt ggtggttata aagttcaagg taaaaaaccg gaacaacagg aagaactttt
    11161 aaaacaagct ttagcagtgg aaaaagcagg tgcctttatg cttgttttgg aaggtatacc
    11221 ggaaatttta ggtaaagaaa ttactgaaca aataaacatt cctacgattg gaattggtgc
    11281 tggaagatat actgatggtc aagttttggt ttataatgat ctgctgggct attcagatat
    11341 gcaacccaaa ttcgtaaaac attatgctga aataaattca aatattaaag aggcgattga
    11401 actctattct caggaagtga aaaatggggt tttccctgct cctgaacata tttattaccc
    11461 tataacttaa atgctgtaaa aagaaggata aaaaagatgc ctaacaaaat aatgccccct
    11521 gaatcaatga gcgccgaaga aaaatttgcg gctttagcta atctgaaaga taaactggaa
    11581 gataattttg taatgctcgg tcagctactt tccgaaataa aacggtctaa actgtaccta
    11641 tttaaaggat atgaaaattt taaggatttt gtagaaacgg aatatcaact ctccggaagc
    11701 ttagcaggaa aacttatttc tactttcgat ctgtttattg aagaaatgga tattgacgaa
    11761 aacgaagtga aagaaattgg ctttgatcgt ttacagatga ttaaaccctt tatgaaaaaa
    11821 gccgattggc aggttcgtga tgaatggatt cataaagcgg aagaaatgcc ttttcaagaa
    11881 cttcgtgatc atataaaaga actaaaagag aaagaaaaag aaaccaatgt agacctaaaa
    11941 gaggtatata ttgagcaatt tatggagaag atgcttggat ggttcaattg ttctcgtaaa
    12001 gagcttaatt ttaagcttgc cctatttttt caagatgctg atctggatca gatgaaaaag
    12061 attgttaaag agcgccaacg tctctttgaa cttgaaacac aaactaacaa ggagtaaaat
    12121 ccaatgaaac aaatgcatat tctttgggtg gatgatgaaa tagacctact gaaacccttt
    12181 atcctctttt tggaggaaag aaactatctg gttgatacct gtaataacgg aactgatgct
    12241 atcgaaaaag tggtagaaaa taaattcgat ctggtaattc tggatgaaat gatgcccggt
    12301 ttagatggtc ttgctaccct tcaagaaatt aaaagaatca atcctgcttt acctatagta
    12361 atggttacaa aaagtgaaga agaaggtttg atggataagg ctattgcctc tcagatttcg
    12421 gactatttaa ttaagcccat aaatcctaat cagattatta tggctattaa gaagatcttt
    12481 caggcagatg aaataagagc taatcagatt ggccaacaat atacgcgtta tattgcagaa
    12541 ttgaatcaaa aactttttgc tactcccgat tgggatgaat ggagccaaat ttatcgtgaa
    12601 ctaagctatt gggaattgca aatagatcag gtaaacgatg aaggtctgcg tcaaactcac
    12661 tttttggaaa agcgaaattg caatactgaa tttactaact atgtggaacg caattataat
    12721 aagtggctga aaagtgatga acggcctaac ttaagttttg atttgatttc tcaacacata
    12781 gctccgcatt ttgaagaaaa tctcccaatc tattttataa ttatagattg tatgcgcctg
    12841 gatcaatatt tggctattca accctatatc caagaacttt ttgatgaaga attggatctc
    12901 tattattctg ttttacctac agctacaccc tatagcagaa attccatttt cagtggttta
    12961 ttgccgattg atattgctaa acgctttcct gattattggg taacttccag tgaaatggat
    13021 aacagccgaa accgtaatga acatcagctt ttagatgaac atattgaaga actgggttat
    13081 aaacttgatc caaccagcaa gtatgtaaaa atattcaata tggaagaagg caacttcgtg
    13141 ctgcgtaaaa tagaaacctg gaacaaagaa aacctaattg ttttggtata taacttcctt
    13201 gatcttttgg ctcatcatcg tagccgggat caaattttac aagaaactat ccccaatgaa
    13261 gaaggtttgc gtgcttttac taaacactgg tttttgcatt cttccctata tgaagcttta
    13321 aaactgattg ccaaacagga tgctattgta attctttcta ccgatcatgg ctcgataaaa
    13381 gttaaccgtg ccactcaagt tatcggagat aaagatactt ctgtaaccgt tcgttataaa
    13441 gaagggaaaa acctttccgt aaatgaccgt catgctttat tcgtaaaaaa acccagtgat
    13501 tatggtctcc cagcaaaaag cattgtggat aactttatct ttgccaaaga cgattactat
    13561 ttcgtgtatc ctaatgctta tcatcaatat cagagacagt ataatggcac tttccaacat
    13621 ggaggagttt ctatggaaga aatgattctg cctattgcta cctgcaggac taaaaaaaga
    13681 acaaaagaat gaaaaaaggt aagaaagcaa gttaaataaa atagtatgat aacaataaat
    13741 ctaccttcag aacaagatac tatagatctt gccaattatc ttgctccaat cttgaaagcg
    13801 ggagatataa ttacccttta tggagatctt ggaagcggta agaccttttt tgttaaacac
    13861 ttgggtaagg aactgggaat caatgaagaa attgatagtc cttcttttat tataatgaaa
    13921 gaatacttag gtggacgcct tcctctttac catcttgatc tataccgttt aaaagataag
    13981 gaagaacttt acgacttagg gatatttgat attttggaaa aaggggtaac cgttattgaa
    14041 tggccacata tagctgaaag tatcttaccc tatcaaactt tcaaacttga tttccacttc
    14101 gacggaaaat cgaggtgggt gaatgtattt ccagacgacg atcataaaca gtactttgaa
    14161 taaggagaaa aacaaatgtt tactgtacta ctagtattaa tctgtattgc aattgcttta
    14221 tgcctgggtt ggattatctt tgctaaagat aaagcaattt cccgcacctg gggtattgtt
    14281 ggtctttctt ttgttattgg attaacagta tttggctatt tttttatgca aagccgtcaa
    14341 gcaccaacaa gtttacgcgt tgtgggttat gccagtaaac tctttgaatc ggacatcgtt
    14401 aaatggaatc taacaatgca gcgtaatgtt agtaacgatg gcttaaagga tggctacaac
    14461 aaaatgagtg ccgatgttaa tgcctttaaa aagtacttat tggaacaagg aataccagaa
    14521 aaagatatca gcattcaacc tacaaatagc tatcaaacaa acgataacta tggcaaccct
    14581 actggttatt gcttaaatca acatatcttt gtggtttcta cagatataga caaaattgaa
    14641 aaactctcct tgaatccaga tttctttgct gaacgcggaa tactcttgca aaattctaat
    14701 cttgcttatc tctatactaa gctgccagat ttgaaaaaac aattgctatc tgaagcaact
    14761 aaagatgcag tagctcgagc tgaagaaatt tcgggaagcg gaaatactaa attaggaaaa
    14821 ttgagagaag cacgagctgg agtttttcaa attactgaac cctattcaac agaagtatcg
    14881 gattatggat tttataatac aagtacaaga agtaaaagca tttccgttac tcttaccgct
    14941 atctttaagc taaagtagaa atatttccac aagcaaaata caatattctg actaatttac
    15001 ttgttagcca aaggggatcg tttagattat atttaaaaga tcctgtatcg aagcaagtgg
    15061 aaatgaataa gaaccttttt gttctaactc ttttgctaac aataatattg ttagttgcgt
    15121 cctgttctcc tgagcagaaa aaaatcctca gagtgggcac agatgctgaa aatcctccct
    15181 ttacttatct ggaaaatagt gaatttaaag ggatagatat cgatatatgt gaaaaaatag
    15241 cagataaaat ggggatgcaa ctgcaaatta cccagtatga atttgatgac ttattctctg
    15301 cattagctca aaacaaaatt gatcttgccg catctaccat aacaattaca gaaaaaagaa
    15361 aacagacatt tgaattctca gaaccctatt ttcaaactaa tttagctgta gtagcaagaa
    15421 aagcctctcc aattgaaata aataaaattg aagatattgg aaaatataga gttggctgtc
    15481 aaagcagcac taccagctac caatatttaa aagaaaacct ggtcgataaa gacctcctac
    15541 ctaaggagaa attgaaaaca tactctacct tgattgaaac cataggagaa ttactcaata
    15601 agaaaataga cctgatggtc tttgatgaat gggttgccaa aaaggtttcc gaacaagtag
    15661 atataaaaat agtatatatc atcccaacca atgaacagta cggactggct atgcaacctg
    15721 gaaaagaaat taatttaaga atcaataaag cactgaaaga attaaaagac agcggtgaac
    15781 ttcaaagaat tataaacgaa tatataaagt aggggtctat actgaaaccc tgaaagctca
    15841 ataagctcat aattgacctt atacctatca cactttttag cgctctatat ttttttcatt
    15901 tgcaaggctc cggtaaaaca taattgagtg catagtgagc atccaacaca ttgatcgtta
    15961 tcaatttttg gaaaacaatc ctcatcaagt tctattgcca gatagggaca gcgagtgcaa
    16021 tttccacatt ttaagcataa ttccctgttc agagaagagc tctttatttt tttaggcagg
    16081 ttcataaaat ctatgatagg attgggctga gcaattccta ccaattcagc cacacttttt
    16141 aaacctcgcg cttccaacag ataggaaagt cccattttca gttcttctat tatgttgtaa
    16201 ccgtattttt ctacaattgt acaaaactga acatttcttg cccctaaagc taaaaagttt
    16261 gctgcatcca gatagttcat tgcccctccg tttccagaaa taaagatgcc ttggtttcca
    16321 gcgcgggcta aatttaaata actgatgggt aatacacctt caccactcat tcctacaatt
    16381 attccttcat cccagggttt ttctttttcg cgaaaagcta aagctggaaa agtattagct
    16441 aaagtgatac ctgccttttt atgaggatat ttatttaaca catttttgat ggctgaaagt
    16501 attggataga tagaagttac cgcaccggtc agtttaaaaa gtttcggtat ttctggagca
    16561 cttatttcca ttacccaatc tactattttt gcagttaaaa caggatcttg agcaacaata
    16621 tcacctttag ttccatctcc accctgagga caggataaac taaattcaat tcccattgct
    16681 cctgcttgtt ctaatttttt agtattggat tgccagcatt gtttatcaga acaatcatta
    16741 ccggtaacag gacctccagt agaagccatt gttaagcgat caggaaattc ttttattaag
    16801 cgagctattt ctttgcaaac tctgtttaaa gaatgctctg aaacattatc acaattggca
    16861 aaggtcttct ttttatcgcc aaaagtgaac atatattcac ccggaatatg aatatcttga
    16921 tcatcaaaag cggttttcat tatgcctcca ctccaaccag cttgatatgc tttttgcatt
    16981 tgctcaaaac catcagttgg aggagccgca gataaaataa aaggcgaatc cattattcta
    17041 ccaaaaaaat cagtttctaa ggaaacagga tattttttcc acccccaaac agggaaacaa
    17101 cttttagttt ccttttccgg tttttctatt gctttgttac taagagataa gtgtacttta
    17161 atagcagtat tttttcctga ggctactgct tcaacaacgg ttgtgggtcc attaaccata
    17221 tctccacaac aaaaaatatt atcaaacttt ctttccagct cgtgaacatt aaaagtagct
    17281 ctattcccaa tagctataat aaccatatca aatatgcggt agttatcaat tactccagct
    17341 tcatctttaa tcagattggg tgcgaatttt tcattcttgg gtagatagac ttttttagtc
    17401 tttattcctt ttaagtgacc attttcattt actatttctg atatccgggt gcgaggagaa
    17461 aattcaattc ctgcctcaat taaagttgct ttctccctat ctgtgattgg catttccaca
    17521 aaattctcca gcgtaatcat ttctacataa gcagcactat ttttagctgc cactaatgca
    17581 atatcggcag ctatggctcc tccaacaata gctatgcgtt taccagaaaa attatatttt
    17641 gatgggtttg aaagaatatc taaaccatag atagctgttt catcaccagg gatgtttaaa
    17701 cgaattggta catttaatcc acttgctaca attactgctg cataaccttc tttcaagagt
    17761 tcttcaggtt tttctatctt ttttggaata tgctgcaatc ggaaggtttt tactacgaaa
    17821 ttcaggtctg ctattatatc aatttcttct aaacgctctt ccgaaatcaa atttatctgt
    17881 cctcctaaag gttcaggatc aaacattctc gtttcgtagc caaattgatt taaagtaaca
    17941 gcagctccaa ttgcagaagg tccagcaccg acaatagcaa tttttgtgtt tagacgaatt
    18001 ggagatgaaa attctggcac caaatccaat tcacgcgctt tgcgaattat agttgcttgc
    18061 acatcaggaa tttggatcgg cttatcgaat tttttatgtg aacaagcatc tacgcaatga
    18121 taatcagggc atacatttcc gcaaattcct cctaaaggat tgctggctaa aattatccct
    18181 gcagctcttt taaaatccga aggtcttccc ttggctaaag ccatcataaa atctgctgga
    18241 gaacaatcac aagggcaggc agctttacaa ggtttatttt cacaatattc acagcgactt
    18301 atttcactaa ataattgagc atctgttaaa atcatttatc ctcccatatt tctagccacc
    18361 aaaagtacaa aaatcaccaa aataaatgag ccaagcagaa ttatcaggac acggaataca
    18421 gaatttcatt ctcgaaagtg tccttcacca atttatgaat taggaatcaa ctttgtgtag
    18481 acggagctcg ctgaagctct gcattttttc attttgcgga agaacagcgt cctccgacta
    18541 cactttttat atcattatct cactaatcta ttatatgtcc tttaccaact cccctaacag
    18601 cacagcccaa agctttgtat ttattaatag tttcatcgga taagaaatta gaacaatcta
    18661 cgattaaggg tttcgttttg cagatagcca aaagttgttc tggatccaaa tttctgtatt
    18721 gactgtgccc aacggcaaaa ataactacat cagattcggg taaaacatta ttcaaatcgc
    18781 tctgtatatg attttcttcc atttcaggcc aggattctac atagggatcg tgagttttaa
    18841 caatagccag gtctttccgt aaaaattcca ctaagatctt ggatggtgaa tgtctcgtat
    18901 cgccaacatc ttgtaaatag gaaacgccta aaactgcaat cttcaaattt tttagttcgg
    18961 gatacacgct ttttacgata tcaatagtat gcaagggcat agtgtcatta gtgttaacag
    19021 cttttatggc aaaatctaaa gttccttcca ctccaaaaag agaattcata gcccaattag
    19081 ctagcacagg gtcttttgtt aaacaatatc cgccaactcc taaagatggt cttaacagat
    19141 tatcatgtgt tcctttgcgt ttacgaatcg cctcgcgcac tttaaaaata tccacgccaa
    19201 tctgttcagc aaattttgcc cattccaaag taagagcaat attcacagcg cgataggaat
    19261 tttccattgt tttggctaat tcactggcat ttgtgctatc caactgagtt aatggaaaat
    19321 gttccacatc taatacattg gaaagaaact ctctacaaag ctctatgctt ttagtattca
    19381 ctcctgaata cacacgccaa aaattacgaa tggattcaac ataatgtgct ccaggcatca
    19441 ccctttcata agaatgagca ataaggggtg gttgattgtt gatatcaatt ccgcgcttag
    19501 taaattcttc ttccaaaatc ggtttgacta cttgttcaca ggttccaggt ggaacagtcg
    19561 tttctacaag cactaaacat tctggctgaa tatacttacc taaagtgcga atgccttctc
    19621 ggaatgcagt tatatcacaa taaccttttt ccgcatctcc aaaagcaggc tttgtagcat
    19681 ccaactgaat atctactact acaatatcta ctaaactgta aacactctct tcactggtag
    19741 cacggaaatt tttcttctct actacggttc gatgaaatat ttcaggaact tctgggtcag
    19801 atgaatttat gggtggaacc ccattattga taacaggaac tttccaatag gaacgttgag
    19861 aagcacgttg atgaccatgg acaaaataga tcgggtttcc atctttatct gtagcatcag
    19921 caacgacagt agccattaca caacctacaa aacctaaacc ttgaacagca actattttac
    19981 gacccaagga acgttgttca gctgtaatac gctttaaagt ctcgcgttct ttttcagcgt
    20041 cttcttcaat gggaagcgga tattcttttc catctggggc aatcgatact tttaacatac
    20101 ttttggttat ctcctttctt tggcattttt ctatcgagaa tccgaaagaa ctacatccta
    20161 ccgttccact atcaacttaa ttatgctgtt tgtgtaataa taaactatac cttaacataa
    20221 ttggtcctct gagaacaaga tgctcacttt cttctcctta tccagttgtt accacttcaa
    20281 tttttagcat ttacctgcac tttgtttttt accttttttg cattgccatt attgtcaaga
    20341 ggtttttctt ttttctgtgg gtggtaaaag taaaaagtca tctcctttag ctcttattcc
    20401 tattaagatt catataagta taaattacaa ttggaaggat taaagttgta acttaaaaga
    20461 ttacgaaaac tccccttttg aaaatgaatt atacttactg taacaatcaa ttttataata
    20521 aaatcccaat attgctgtct cctattttcc ttgcttcaat ccattaatct atcgtttatc
    20581 taaccttaat caagcgttaa taattaacgc ttgataaagg gttgattaac acttgattaa
    20641 cgctttccct taaggccaaa gagcgaagct tgaatacaat atgcagttta tactatagta
    20701 gtaaatacct gatgacagat atatgtaata ggatagtact taaatatcca cccttcattt
    20761 tactgtctaa ggaaaaaaga gcttaccatt ttttgctggg cacataaacc gaagtttgtc
    20821 aacagactat atttttacct ttttttagaa tgattttatt cggcggttca gttgctgatt
    20881 atgtccataa actatgtggc tttcttatgc ggatttttac tcagaggaaa attccggatg
    20941 aattgtagaa acagaatatc acttcctcct tagtaattta acttgataaa aaagaagaac
    21001 acctttttcc ttttactctc tatttttcct tcttgtggat atctttgaaa gatatagcta
    21061 tcttaataac gcataaatta ggatggtaaa gcaaaaagag atattaaagg agtaagttat
    21121 agtgatgatt ttcatttgct atctgggttg tcgagacaaa gaaaggaagg taaataggtt
    21181 gtttccaata attttctcat atcctagcaa aagaggatta tcagcaaaaa gcaagctgta
    21241 aacaaaaaaa gattacgaat ataaatggct acaaaataac taatgcagta caacttagtg
    21301 ttgttttatt attactatgc ctttataaac aatcaatctg aaaagtaaaa tttaaggttt
    21361 catactattt tcaactagat ttcattaaaa agtatatggt aaatagtatg ttctatgaga
    21421 aaatcttgct ttgtaaaggg gttaaaaaat acaactagtg ctaattgtaa caagttgaaa
    21481 acataattag ttacaaataa tcagattttt ttataataat tt
//
LOCUS       DAOSTH010000022        19688 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_2930_length_19688_cov_12.379820, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000022 DAOSTH010000000
VERSION     DAOSTH010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 19688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19688
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2930_length_19688_cov_12.379820"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            169..1113
                     /gene="cas6"
                     /locus_tag="PLB65_04425"
     CDS             169..1113
                     /gene="cas6"
                     /locus_tag="PLB65_04425"
                     /inference="COORDINATES: protein motif:HMM:NF021551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR system precrRNA processing
                     endoribonuclease RAMP protein Cas6"
                     /protein_id="HOQ77807.1"
                     /translation="MKYLELKAFYIIDQPIGMIADLSSALRGLWGRSLRKIYCYQKQL
                     ECSECPLENCTYYVLFEKKLSSSDQYHPYIIQSRVVNPYLIEAKFKFFGWVCDHLEKL
                     LYSIINTNNTLLKREGRNHCLTLQKIEDMNSNRIYSAESEIITRPKLKKLSFSPQEVP
                     LLELIFKTPLRQKYQGKLMNEFIWEPFAKSLINRIRFINEHFNSNELNIPEQINIEGT
                     TKKKENTYWSEKIRVSFRQESKMSIGGLLGSVVLANVSPEMIGMLKLGRYLHTGKQCT
                     FGNGEYVLRNLSENQSVTEDTAELKQNKINNLSDKSPP"
     gene            1283..3784
                     /gene="cas10"
                     /locus_tag="PLB65_04430"
     CDS             1283..3784
                     /gene="cas10"
                     /locus_tag="PLB65_04430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Cas10/Csm1"
                     /protein_id="HOQ77808.1"
                     /translation="MSLSIHHIVFAALMHDVGKVIQRSGASDVPKYITKCKEDPKYGY
                     YTYKHAMWTADFLDRHPLNLPDKNWESIVELAGSHHCKDSWKLHNYEKYLDMIMQADN
                     ISSSWDREEPEDPSSDKHHYLKVPLYSVFSKISTGENKIEKNAKKWIYPLRKLNPENA
                     YPAESKDLVDQSAAYKALLTGFEEDYGKLCKYYNELADKNKVNFETEHFPQFIDAVDS
                     LLRTYFWCVPANTMEPTPIGSLYHHLHNSAAISAALYAQDESEHSEPPFMIIAADLNG
                     IQNYLYDLNPENSSKASKLLRSRSFQIQMIMEMLADKVISELGLSHLSVISSQGGKWF
                     ILANNSKNNEQKMEELKQIVNEEMFTRFLGQISINLSWETKLGLFDLKKEHFIGTMNR
                     VYDSLEKEKTHRFSTYLQKDNLWQTENFVINHDNIYSDQICDFCKRRNCDEDSNKIYK
                     EKENPEEENPRVCHHCLQEIRLGRNLTKYKYYKIWKGEKDKDAYINFAGMSFSKVKNN
                     ELNSLIAGHHYLALSEENEFLQIPKRYLATQVPIKDGKVTTFDEIPKHNEGLQANAVM
                     KGDVDNLGYIMSRGWQKDDSGNPICSITEYSTLSFMLDYFFSACLPAQIKEKYEDSIY
                     TVYSGGDDFCIIGAYEKVIAFAENLNKKFNEFCAKNPDMHFSAAITLAHPKEPVKFSI
                     NSTELRLKDAKAEPDKNSLHLYETIVQYDKLSDLLEFADKLKNWLDQEELTLQFLYRL
                     LTYHQMYLETLNKDNVNYRNYLYESLLNYDIKRNIEKMKDNKLTNLEIVNELRSLTGL
                     EEGSMMKYLRIPLCHTIYKKRNKHKNNKQKENKNV"
     gene            3777..4190
                     /gene="csm2"
                     /locus_tag="PLB65_04435"
     CDS             3777..4190
                     /gene="csm2"
                     /locus_tag="PLB65_04435"
                     /inference="COORDINATES: protein motif:HMM:NF015695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated protein Csm2"
                     /protein_id="HOQ77809.1"
                     /translation="MYNNQQNRNTTPDRGGAKYGPFFKEDGSLNLAWVGEKAQRISQE
                     ISDARYGLTATGLRNFYNEFLRIRNLPASHSEEKKILIKLLIAKVNYKKLNNKAPDIF
                     AKFITELITEVGDDLDKFEKSCLIMEAVVGFNPRK"
     gene            4210..4908
                     /gene="csm3"
                     /locus_tag="PLB65_04440"
     CDS             4210..4908
                     /gene="csm3"
                     /locus_tag="PLB65_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm3"
                     /protein_id="HOQ77810.1"
                     /translation="MLTTKIITGKILLKSGLHIGGNKDTMQIGGIDNPVMKNPLTNMP
                     YIPGSSLKGKMRFLLEHYLNYVKGDGKIPGLTVDGKVNKIAIIFGHLEHTENSSDTKP
                     TRIIFSDCNIIGARDETGNLIEDLEYLKEKMLSNFVEAKTEVAINRITGTAQGGALRT
                     IERVPAGVVFDLHIVLKNYEESDESNLEIIKKGLKLLENDALGGSGSRGSGRIEFLDL
                     KVDGASIDLNSVQL"
     gene            4918..5931
                     /locus_tag="PLB65_04445"
     CDS             4918..5931
                     /locus_tag="PLB65_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77811.1"
                     /translation="MTTWKISWKPLSSLSTPLQSDTIFGHLCWAINYLWEEKDKNKLT
                     DFLSALQETPCLVLSSAFQEGHLPKPSIPIGNKHLDEVKKNLKKCKELKEITDLEISI
                     VLKRIKKDNWIDRKALTDNEYVYNVSEELISKAQDTALKILESRGTRIHSPKKEEGMI
                     EFHNMIDRITGTTSGSGELFASPTVFYKDICFESWLDTDYFNESEIKDIFHFIEQNGF
                     GKDKNTGKGKFEITVEEFHWQDCPKNNAYLNLSNMVPCASDSPIASYNSKTKFGKVGG
                     DYATSKTPFKYPIYIIEPGAVFFAEVRDKKPIGSLLRNIHPTEDIVQNLYSYNIPIMI
                     KGS"
     gene            5934..7037
                     /gene="csm5"
                     /locus_tag="PLB65_04450"
     CDS             5934..7037
                     /gene="csm5"
                     /locus_tag="PLB65_04450"
                     /inference="COORDINATES: protein motif:HMM:NF015728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-A CRISPR-associated RAMP protein Csm5"
                     /protein_id="HOQ77812.1"
                     /translation="MKPLKYPFRISPITPFHIGNGTQYDPLNLIIKNGTAYFINQIEY
                     IRYLMEKDKKKLDRNLEVSNIKQLHKFFAESFDPNAKDTFYFSYPVKKDICDTYAKNM
                     ENLQNQGFIQAFIRSTLNMQPYIPGSSIKGAIRTAVLSNICSDKNKIIQDRNTDKADQ
                     KTQAVLLEYWNREKNSVDVPSDPFKFIKFEDIPWKNDWIRIFGVEITTPPVSASVRQN
                     PFHSGNQSEKEKKEPPIPVLMEVALSRIEWVVDSELTVLVTDKNNRGFNKIMPSGPND
                     IKPLIKMVNNYYQQQIDRESEYYKSMNPNAQKNYKAIKGLFDNLKDNECIIKLGMGTG
                     QNYCSYAVMNYQPKSRRMINSLPMGWMRLTFSI"
     gene            7046..8137
                     /locus_tag="PLB65_04455"
     CDS             7046..8137
                     /locus_tag="PLB65_04455"
                     /inference="COORDINATES: protein motif:HMM:NF021189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77813.1"
                     /translation="MNSIILSPVGTSIFTKGKFPSEINQFSNYKNLEDIPSDRRGIIS
                     SYIQEVHDLMLSSSLEDVKKRSAELNGIIHYYNDNLHNAQKDIHYLLPSDTYIGKSAC
                     EIIKCFLDKYIGSVCVLEIKDLQTYDCDRFQYALSDVVSQIMIIKRGLLPEQKFIFNL
                     TGGFKSILCFLQSLGMFYADETVYVFEYSKDLMRIPALPVKLVPYDYLKEYIVVFRRL
                     YNNLSVAEEEYDKIPESLILKLYGEPTLSAYGKIVWDNFRKDLYSEKLLDSISPKVKY
                     SEIFLKSTKDLGKDRYYNLNMRIDEVSCFMELKSKPNPASLDLKKLATPKGISTHEFD
                     AWADKDAKRVFCHYDQDILVLDTLLEGLH"
     gene            complement(8129..10192)
                     /locus_tag="PLB65_04460"
     CDS             complement(8129..10192)
                     /locus_tag="PLB65_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease"
                     /protein_id="HOQ77814.1"
                     /translation="MSQLIISMGKTSYLIAEVVGFFNYEQYPLYYNHKSFETLKETRE
                     KNQLEPINTISMICTNDSFTSNKQAVEDWLKAQHLNIDLQFHILNQLKDISSAEDARI
                     MRNLIYSLVYNCYKQGNSNKLYLCLSGGRKTMSSDIQQAAYLFGCKAMIHILAEGDVS
                     FNLSAAPEDIEPAVINKINPVLYQKDIPASKLAELIEEEKVYFPEPKIKGNEYIYSDS
                     ETKFLNQVEELQTKQDNLTINYYTKLRYSEPSSNFQILHLLNPKKIQELKNNYINGID
                     NIKQDLNWLYKLPKTDLHCHLGGFADTEGLIKIAIANLKYSGNTLEEKKLERSTINAI
                     QKNDLNKLKAIWKTISTRENKVRWLLVSLYLRCFEGKEKLLEQVIWNNLLQEDNFVDI
                     GLENYEEIGDFQGSSLLQTEEALREICHQLKDDAKKNNLRYKELRCSPCNYTNKLKAT
                     EVVEILYDELIDEECVFRLIIIGSRHKTQRILKEHIDLCKNIKRENGKISDFICGFDL
                     AGPEEKTTLSLREKILPLLEDCIRITIHSGETTSVKNIWEAVYNLNADRIGHGLYLQD
                     DENLMLKLKENKTAIELCPSSNFQINSYRDYSINSTSKMRIYPLKQYLQKGIKACICS
                     DDPGISRTDISKEYLKAAKMTKGGLSKWNILTIIRNGFVSSFQTRENKQKLISEAEKD
                     IMKLV"
     gene            complement(10207..11727)
                     /locus_tag="PLB65_04465"
     CDS             complement(10207..11727)
                     /locus_tag="PLB65_04465"
                     /inference="COORDINATES: protein motif:HMM:NF033611.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAVED domain-containing protein"
                     /protein_id="HOQ77815.1"
                     /translation="MPRFRDRVDNYLKNSSSEAENIILQYIQQDRVPQEDEQWVYTLL
                     EKAKNPNMKLNSLMWLSAKGKYLEQVSKLLETSETEINSLSQQQPKIGIFPVVDNSGN
                     ALLARAFIYQLESSEPKAIAILGDKFENYSYLAKLGKQNCIIGFDKNITGNSWQLAVL
                     ATLLSKKDTLFANLAFSGIVLPNGEIIKADEIEAKKRCCPHLVYRVKNCDQLDAWLNI
                     ETIPIPIIQYQGEENELIRWQKALEQKVQENFSWFSYELLEDFYGITTNNLAIFGNGI
                     LPFEKNAWQTLLKEQVKDKFNALEDKIMPKKVLWFYSGQISTLQIGIGALFGFKRAVS
                     ILQMEFSNTTYKEVFTLYGKENARQLKNVSFKKEDFQYIQSELQINDPQKNELGFIIY
                     LGSHNPIGEAKAFCQKNLQVNNFLIIKAKQDQGVMETSQNWLPFVQEINSALNAAREK
                     NHWQRIHLFQTAPIALCMALGIAIGHFVPIDVYHYQFNASEPKYRCMFSMDKILSL"
     gene            complement(11741..12646)
                     /locus_tag="PLB65_04470"
     CDS             complement(11741..12646)
                     /locus_tag="PLB65_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77816.1"
                     /translation="MNEKDKELLYQTYLEGITKETQVPIIELEDIDFVLPEDVEFTFP
                     EFQPHTEQLLGKMIVLETDEYLIDCIVVNKTIDNEYLAYKTSPFTVFASEFDIVFKTD
                     MGKYLLETGNYFYLTEEEIKEAIELDYLQPSNLESLKQELRKEHLVDENRNTDLESPE
                     NKFLLMEYNITLGLRCRHSDPKIWVPQIEIQYRSNPANNVKMAAADMEIAKFAFRLVS
                     TDLFKVHKYAYFADYYTLYKDDCHNLILAPDDNLIDQKAEIRCGDIVVFKGKLPKQIV
                     LANFIPLLPKQAQDFLDITLKVIKD"
     gene            complement(12663..13475)
                     /locus_tag="PLB65_04475"
     CDS             complement(12663..13475)
                     /locus_tag="PLB65_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77817.1"
                     /translation="MNKDTSLIQGYKNWYNNSEDGKDILSLEMALWSVLCEPNLLSSN
                     KIVFYQVYALIQMFLHKRPSQHSMLKNLAYKEGYNTEDTHICLIGRLIMKLTPNLKKL
                     EEKKNQLKSEEDFGKYLFKTGEIVTMHCVEEFINDIVSNKIESIMIEQYYGNYNISKY
                     QDLSYIQYEIVTRHQAILLAEIIKEEWKIREINVLCHDLENNCESGSNRLKNESRDNI
                     YKLHERIRTKLKLTLENNNFDENVGRLFIKEFLKKICQNCPPLPTYKEEKGY"
     gene            14391..16283
                     /locus_tag="PLB65_04480"
     CDS             14391..16283
                     /locus_tag="PLB65_04480"
                     /inference="COORDINATES: protein motif:HMM:NF040561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated primase-polymerase type A1"
                     /protein_id="HOQ77818.1"
                     /translation="MMQEKVTELGSQAILLPENEFHSFRLQFNSLFIKEELNTAHNLA
                     LKTLSSENLNSDELVELARCFQLLGDKDNTLTCLEKAIQIDDQNKKAKVLKLELLDSL
                     EQKGQYLDFLQHCLHNDPQEKQYYLLLHTFYTENGQNELAENVSALALSNGINLVLPN
                     VEIEITGDDFPPDPVAIEDPILLSNYLTLFAGRENCYARQWVSDKGKTGYTPVIEPLN
                     PVLIRNHLQGIQTLGVYQLTLKNQVKWIVFDIDIINDYLDDIHDPHFREWVDNGFLQV
                     LNNFDNILQTFQLRAVYEYSGYKGYHIWLFLQEYTSAAIARTFALKLATQIDISSFPF
                     QIEVFPKQTRTSTNNFGNLIKLPGGVHRFSGLKSTFFTLTDGALEPLPLSSLLKKPPL
                     ISPSDFLSALCSLQPDFSCNTLDSSRENYQTENVNISIIPAEPSPELDPQWLWLKQRC
                     SALAFICQKIEAHHQLNDAQKKVLIHCVGHLDRGPEIVNFYLRQCNPCDPSDFLKSTL
                     KGNVISCNKIRSYLASENDSFDCDCDFSGKIFSYEHPLIHLEDYVLSSSPFSGNNELL
                     LKDTISRYLDIKRRNNELSETLQHLEKHILELFEQIGVSEFKTPYGVLKKDNTDNNVK
                     LILELV"
     gene            16293..17651
                     /locus_tag="PLB65_04485"
     CDS             16293..17651
                     /locus_tag="PLB65_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77819.1"
                     /translation="MNENRDAVNNAWNLIKQGNYRDALAVLEAYSPNDLSSHYFLASL
                     LLECYNKLEMYNKAINCGENLCTQKNCSPSIKQNYAWSLYFAYFKKNEVLPLEEAIPL
                     IERIASLTDCTKKVNPLVLTIFSYLTKNKQLSPEIIIQLLDMLKFDLLDDKAVNGKNK
                     YQTFPSQKEKYICYYTKALFLEKNYSRCIACCKRALELSTNYDNFKNKGNPSEDASLC
                     LPDVKNALFISPANLIWIKRYLALSLFYLGEYEQAYLLMQEIIKQKKDWFLYYELSQI
                     TQKLNKIEDALRYAAIAAYSLGDIEMKIHLWEYLYQLLLQTKNFPQAVKILSLSAAVR
                     AQKGWKISPSLKQEIDKHNLQPENLPSYKNIFTELKPWFIEMSYSGSEVLTGYISNIL
                     ASNKAGFITTGKKYYYFKTSSCLFEPEYIVPNTKVSFNTEKSFDPKKQKDSIIAVNIK
                     RI"
     gene            17654..18688
                     /gene="cas1"
                     /locus_tag="PLB65_04490"
     CDS             17654..18688
                     /gene="cas1"
                     /locus_tag="PLB65_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOQ77820.1"
                     /translation="MPVVYIADQGSHLCKRGDRLYVYRGTQLLRWFHTKDIIQLILVG
                     NIGLTSQVVTYLLKYRIDTVFLSYYGKFKGRLVGEFGKNVLLRVNQFQYLSDLENRTL
                     LANLIIRGKIANSLFHLAKRKKRNKEESITVAYIKNKALLAQLNSQIYPKDILLGYEG
                     IAAKNYFAAFPALIANNDFPFSGRNKRPPKDEVNAMLSLSYTFLMNQVMCSAYICGLD
                     PYYGALHDLDYGRQSLVLDVMEEFRPLIDNMVISLINRKEIRLEHFLYNTLPNDEDPE
                     EENSVLPVSLTKDGMKIIITAFSKLINSKFPLQDLPGEWSMKDIITLQIRKLAAHFEN
                     KKEYLPFLWM"
     gene            18762..19052
                     /gene="cas2"
                     /locus_tag="PLB65_04495"
     CDS             18762..19052
                     /gene="cas2"
                     /locus_tag="PLB65_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOQ77821.1"
                     /translation="MSLNKHLYIVAFDIVSDKIRYRCVKILLSYGQRVQKSVFECILN
                     DKQFLEMKSKLDNTINLEVDSVRYYLLCANCEKNISVSGTGLYSYDEELIII"
BASE COUNT         6322 a         3418 c         3391 g         6557 t
ORIGIN      
        1 atggggttca agtggtcgca ggttcaaatc ctgtcatccc gaccagtttt ttttatacca
       61 cagaagatac tgaaaactct gagcgtactg agaactaatc cacctacttt tcactttttc
      121 cttgacttaa agatagcaaa tattttccta ttgttttaag aggaaaatat gaaatattta
      181 gagttaaagg ctttttatat tattgatcag cctataggta tgatagcaga tttatcttct
      241 gcccttagag ggttgtgggg aaggagttta cgcaaaatat actgttatca gaagcagttg
      301 gaatgtagtg aatgtcccct agagaattgt acttattatg tcctatttga aaagaaactt
      361 tcatcttctg atcaatacca tccctatatc attcaatcca gagttgtaaa tccttatctg
      421 attgaagcaa aatttaagtt ctttggctgg gtctgtgatc atctggagaa acttctctat
      481 tccattatta ataccaataa cacacttctg aaaagggaag gaagaaatca ttgtttaact
      541 ctgcagaaaa ttgaggatat gaattccaac aggatttata gtgcagaaag tgaaataatc
      601 acccgaccca aactaaaaaa gctatcattt agtccgcaag aagtgccttt attggagctt
      661 atcttcaaaa ctcctttacg ccagaaatat cagggtaaac ttatgaatga atttatttgg
      721 gagccctttg ccaaaagttt aatcaataga attcgcttta tcaatgaaca cttcaacagc
      781 aatgaactga atatcccaga acagattaac atagagggaa caaccaaaaa aaaggaaaat
      841 acctattgga gcgaaaaaat cagggtttcc tttcgtcagg aaagtaaaat gagtattggc
      901 ggtttgctgg gcagtgttgt tttagccaat gtttctcccg aaatgatagg tatgctaaaa
      961 ttgggtagat atttgcatac tggcaagcaa tgcacttttg gtaacggcga atatgtattg
     1021 cgtaacttat cagaaaatca atctgttaca gaagatactg cagaattaaa acaaaataag
     1081 ataaataatt tgtcagataa atcccctccc taaacatata actaaatagg aatccaagat
     1141 ttcaaaaata aaatacttct attggaggta acaaatgaaa aacggaccta tcagaatcat
     1201 cattattgtt gcaaccgcta ttgcagctat tggaaaatca atccttgaaa cagcaccaaa
     1261 agctaaaaaa taaggagtga ttatgagttt atctattcat catatcgtct tcgctgctct
     1321 gatgcatgat gtagggaaag taattcaaag atcaggagct tccgatgtcc ccaaatacat
     1381 aactaagtgt aaagaagatc caaagtatgg ttactatacc tataagcatg caatgtggac
     1441 tgcggatttt ctggataggc atccactcaa tttaccagac aagaactggg aatcaatcgt
     1501 agaattagct ggatctcatc actgcaagga ctcctggaaa ttgcataact atgaaaaata
     1561 tttggatatg attatgcaag cggataatat ttcttccagt tgggatagag aagagccgga
     1621 agatccatct tccgataaac atcactatct gaaagtccct ctctattcag ttttcagcaa
     1681 gataagtacc ggagaaaata agattgagaa aaatgctaag aaatggatat atcctttacg
     1741 aaaattgaat ccggagaatg cctaccctgc agaaagtaaa gatttagttg atcaaagtgc
     1801 tgcatataaa gctcttctga caggttttga agaggattat ggcaagctgt gtaaatacta
     1861 taatgaatta gctgacaaaa ataaagttaa ttttgaaacg gagcattttc ctcaatttat
     1921 tgatgctgta gatagtttgc tgagaactta tttttggtgc gttccggcaa atactatgga
     1981 gccaacccct attggttctc tttatcatca tctacataat agtgctgcta tttctgctgc
     2041 tttatatgct caagatgaaa gtgaacatag tgaacctccc tttatgatta ttgcggctga
     2101 cttgaatgga attcagaatt atctttacga cctcaatccg gaaaattctt ccaaagccag
     2161 taagctcttg cgttcccgtt cttttcaaat tcagatgatt atggaaatgt tggctgataa
     2221 agttatcagt gagcttggtt taagccattt gagtgttatt tccagtcagg gtggtaagtg
     2281 gtttattctt gccaataact ctaagaataa tgaacaaaaa atggaagaac tgaaacagat
     2341 tgtaaacgaa gaaatgttta ccagatttct gggacagatt agtattaatc ttagctggga
     2401 aactaaactg ggcttgtttg acctgaaaaa agaacatttt atagggacaa tgaatcgcgt
     2461 ttatgattca ttggaaaaag aaaagaccca tcgtttttct acttatctgc aaaaggataa
     2521 tctctggcaa accgaaaact tcgttatcaa tcacgataac atttattccg accagatttg
     2581 tgatttttgt aaacggcgta attgcgatga agattcaaat aagatatata aggaaaaaga
     2641 aaatcctgaa gaagaaaatc cccgtgtttg ccaccattgc ttacaggaaa tacgcttagg
     2701 cagaaatctt accaaatata aatactataa gatctggaag ggagagaaag ataaagatgc
     2761 ttacattaat tttgctggta tgagtttctc aaaagtaaag aacaatgaac tcaattctct
     2821 aatagctggt catcattatc ttgctttaag tgaagaaaac gagttcttac aaattcctaa
     2881 acgctatctt gcaacccagg ttcctataaa ggatggcaag gtaactacct tcgatgaaat
     2941 acctaaacac aatgaaggtt tgcaagccaa tgccgttatg aaaggagatg tagataatct
     3001 tggctatatt atgagccgcg gttggcaaaa agatgattct ggaaatccca tttgctccat
     3061 caccgaatat tctaccttgt cctttatgct ggattatttc ttcagcgcct gtcttcccgc
     3121 tcagataaaa gagaaatatg aggattccat ttataccgtc tattccggtg gagatgattt
     3181 ctgcataatt ggggcttatg aaaaagttat agcttttgct gaaaacctaa ataaaaagtt
     3241 taatgagttc tgtgccaaga atccggatat gcatttctct gctgcaatta ccttggctca
     3301 ccccaaagaa ccagtaaagt tctcaattaa ctctacagaa ttaagattga aagacgcaaa
     3361 ggctgaaccg gataagaatt cacttcatct gtatgagaca attgtccaat atgataaatt
     3421 atccgattta ttggaatttg ctgataaatt aaagaattgg ttagatcaag aggagctaac
     3481 tcttcaattc ctatatcgct tgctaaccta tcatcagatg tatctggaaa ccctaaataa
     3541 ggataatgta aattatcgca actatttata tgaatccctt cttaactacg acattaagcg
     3601 gaatatagaa aaaatgaagg ataataaact tacaaatttg gaaattgtaa atgaacttag
     3661 aagtttaacc ggcttggaag agggcagtat gatgaaatat ctaagaatcc ctctttgcca
     3721 tacaatttat aaaaaacgca acaaacataa gaacaataaa caaaaggaga ataaaaatgt
     3781 ataataacca acaaaatcgg aataccacac ctgatagagg tggtgcaaaa tatggaccct
     3841 tttttaaaga ggatggttct ttaaatttgg cctgggttgg tgaaaaagca cagagaatct
     3901 cacaagagat atctgatgca agatatggtc ttacagcaac gggattaagg aatttctaca
     3961 atgaatttct tagaatacgc aatttacctg caagtcatag tgaagaaaag aagatattga
     4021 tcaaattact aattgctaag gttaattaca aaaagttaaa taacaaagca ccggatattt
     4081 tcgcgaaatt tattactgaa ctaattaccg aagtcgggga cgacttggat aaatttgaaa
     4141 agtcctgttt aattatggaa gccgtagtag gtttcaatcc cagaaaataa atttataagg
     4201 agaacgaaaa tgttaacaac taaaataata accggaaaaa tactgctgaa aagtggttta
     4261 cacattggtg gaaataagga cacaatgcaa attggtggaa ttgataatcc tgtaatgaaa
     4321 aatcctttaa ccaatatgcc ctatattcca ggttcctctt taaagggaaa gatgagattc
     4381 ttgctggaac attatttgaa ttatgtaaaa ggtgatggta aaatacctgg tttaacagta
     4441 gatgggaaag taaataaaat tgcaataatc tttggacatc ttgagcatac cgaaaatagt
     4501 agtgatacaa aacctacccg tattattttt tccgattgta atataattgg tgcaagagat
     4561 gaaactggta atttaattga ggatcttgaa tatctgaagg aaaaaatgct ctccaatttt
     4621 gtagaagcaa aaacagaagt agcgattaat agaataactg gcacagctca gggaggagca
     4681 ttaagaacta tagaacgagt tcctgcagga gttgtttttg atttacatat tgttctaaaa
     4741 aattatgaag aaagcgatga aagtaatctg gaaataatca agaagggctt gaagctttta
     4801 gaaaatgatg ctttaggggg atcaggttcc aggggcagtg gtagaataga atttctcgat
     4861 cttaaagttg atggagcttc tatagatttg aattcagttc agttatagga ggtcgtaatg
     4921 actacctgga aaatatcctg gaaaccctta tcttctcttt ccacgccttt gcagagcgat
     4981 acaatctttg gccatctttg ctgggctata aattacctgt gggaagagaa agataagaat
     5041 aaacttacag atttcctatc tgctctacaa gaaactcctt gtcttgtttt atcttctgcc
     5101 tttcaggaag gacatttacc taaaccttct attcccatag gaaataaaca cttggatgag
     5161 gttaaaaaga atctaaaaaa atgtaaggaa ttgaaagaaa taacggattt ggaaatttcc
     5221 atagtgctga agagaataaa aaaagataac tggatagata gaaaagctct cacagacaac
     5281 gaatatgtat ataatgtgag cgaagagctt atctcgaaag cacaagacac agcactgaaa
     5341 attctggaaa gcagaggaac ccgtattcat tcacctaaaa aggaagaagg aatgatagag
     5401 tttcacaata tgattgatcg gataacagga actacttctg gaagcggaga actttttgcc
     5461 agtccaactg tgttttataa ggatatctgc tttgaatcct ggctggatac tgattatttt
     5521 aatgaatccg aaatcaaaga tatattccat tttattgagc agaatggttt cggtaaggat
     5581 aaaaacactg gcaaaggtaa atttgaaata acagtagaag aattccattg gcaggattgt
     5641 cctaaaaata atgcttatct taatctttcc aatatggtcc cttgtgcttc agatagccct
     5701 attgcttctt ataattccaa aactaagttt ggcaaagtgg gtggagatta tgctacttct
     5761 aagaccccct tcaaatatcc aatatacatt attgaaccag gagctgtatt ttttgctgaa
     5821 gtaagagata agaaacctat aggttcatta ttaaggaata ttcatcctac tgaggatatc
     5881 gttcaaaatc tatattcata taatattccg attatgatta aagggagcta aaaatgaaac
     5941 cgctaaaata tccctttcgt attagcccta taactccttt tcatattggc aatgggaccc
     6001 aatacgatcc tttgaacctc attattaaaa atggaacagc ttacttcata aaccaaattg
     6061 agtatatccg ctatcttatg gaaaaagata aaaaaaaatt agatagaaat cttgaggtat
     6121 ctaatatcaa acaacttcat aaatttttcg ctgaatcgtt tgatcctaat gccaaagata
     6181 ctttctactt ttcttatcca gtgaaaaaag acatttgtga tacttacgct aaaaatatgg
     6241 agaatctcca aaatcaagga tttattcaag cttttattcg ttctacctta aatatgcaac
     6301 cctacattcc gggaagttct attaaaggag ctattcgaac agctgttctc tctaatatat
     6361 gttctgataa aaataaaatt atccaagata gaaatacaga taaagctgac caaaaaactc
     6421 aagcagtgtt acttgaatat tggaatagag aaaagaattc tgttgatgtt ccatccgacc
     6481 ccttcaaatt tattaaattt gaagacatcc cctggaagaa tgattggatt aggatttttg
     6541 gagtggaaat tacaactccg cctgtatctg caagtgttcg ccaaaatcct tttcatagcg
     6601 gaaaccaatc tgaaaaagaa aaaaaagaac cacctattcc cgtgctaatg gaagtagctc
     6661 tctccagaat tgaatgggta gtggattcag aattaactgt tttagttaca gataaaaata
     6721 accgcgggtt caataaaata atgccatctg gtcctaatga tattaaaccc ttaataaaaa
     6781 tggttaataa ctattaccaa cagcagatag atagagaatc agaatactac aaaagtatga
     6841 atcccaatgc gcagaaaaac tataaagcca tcaagggttt atttgataat ttaaaagata
     6901 atgagtgtat catcaagctt ggtatgggaa caggtcaaaa ttattgttcc tatgccgtaa
     6961 tgaattatca acctaaaagt aggagaatga tcaattcttt gccgatgggt tggatgagat
     7021 taacttttag catataggag aaaaaatgaa ttctattatc ctatcgccgg tcggaaccag
     7081 tatttttact aaagggaaat tcccttctga aatcaatcaa ttttctaact acaaaaattt
     7141 agaagatatc ccttccgacc ggcgagggat aatttcttct tacattcaag aggtacacga
     7201 tttgatgctg tcttcctcat tggaagatgt aaaaaaaagg tctgccgagc ttaatggcat
     7261 tattcattat tataatgata atctgcataa tgctcaaaag gacattcatt atttattacc
     7321 gagtgatact tatattggca aaagtgcctg tgagattata aaatgctttt tggataagta
     7381 tattggcagt gtttgtgtgt tagagataaa ggatttacag acttacgatt gtgatagatt
     7441 tcaatatgcg ttaagtgatg tagttagcca aataatgatt atcaaaagag gtttacttcc
     7501 tgagcaaaaa tttatcttta atttaacggg tggttttaaa tctattcttt gttttctgca
     7561 atcattaggg atgttttatg cggatgagac agtttatgta tttgagtatt caaaggattt
     7621 aatgcgtatt ccagctttgc ctgtaaagct ggtcccttat gattatctga aagaatacat
     7681 agttgttttt cgccgtttat ataataattt gtctgtagcg gaagaggaat atgataaaat
     7741 accggaaagt ttaattctaa agctatatgg tgagccaact ctttcagctt atggaaaaat
     7801 cgtttgggat aatttccgca aagacctcta ttcggaaaag ctactggatt ctatcagtcc
     7861 gaaagtaaaa tattctgaga tttttttgaa gagtaccaag gacttaggga aagaccgtta
     7921 ttataattta aatatgcgta ttgatgaagt cagttgtttt atggagttaa aatcaaagcc
     7981 caatcctgct tctttggacc taaaaaaatt agcaacccca aaaggtatta gcactcacga
     8041 atttgacgcc tgggcagata aagatgctaa aagggttttt tgtcattatg accaggatat
     8101 ccttgtatta gatactcttt tagagggttt acactaattt cattatgtcc ttttcggctt
     8161 cgctgattaa tttctgctta ttttctctgg tttggaaaga gctcacaaaa ccgttgcgga
     8221 ttattgttaa aatattccat ttgctcaatc cgcctttagt cattttagct gcttttaaat
     8281 attctttgct tatatcggtt cgcgaaattc ccggatcgtc agaacatata caggccttta
     8341 tccctttttg caaatattgt ttcaaaggat aaatacgcat tttggaggta gaattaatac
     8401 tatagtctcg atagctgttt atttgaaaat tgctgctggg gcaaagttca atagcggttt
     8461 tattttcttt aagttttagc atcaggtttt catcgtcttg caaataaagt ccgtgaccaa
     8521 ttctatctgc attcaggtta taaactgctt cccagatatt tttcacggaa gttgtttctc
     8581 cagaatgaat agtaattcta atgcagtcct ccagtaaagg taatatcttt tctcttaggg
     8641 aaagggttgt tttctcttcg ggtcctgcca aatcaaaacc gcaaataaaa tcgctgattt
     8701 tcccattttc tctttttata tttttacaaa gatctatatg ttcttttagt attctctggg
     8761 ttttatgtct gctgccgatt attattaagc ggaaaacgca ttcttcatct atcaattcgt
     8821 catataaaat ttctaccact tcagttgctt ttaacttatt tgtatagtta cagggtgagc
     8881 accttaattc tttatagcgt aagttattct tttttgcatc atctttcaat tggtggcaga
     8941 tttctcgtaa tgcttcttct gtttgcaata gggaagaacc ctgaaaatca cctatttctt
     9001 cataattttc tagtccaata tctacaaagt tgtcttcctg caaaaggttg ttccagataa
     9061 cctgttctag taatttctct ttgccctcaa aacagcgtaa atacaaactg acgagtaacc
     9121 agcggacttt attttccctt gtacttatcg ttttccagat tgctttaagc ttattaaggt
     9181 cattcttttg gatagcatta atggtacttc tttccagttt tttttcttcc agggtattgc
     9241 ctgagtattt taaatttgct atagctattt taattaaacc ctcagtatcg gcaaaaccgc
     9301 ctaaatgaca gtgtaaatca gttttgggta atttatatag ccagttcaaa tcttgtttga
     9361 tattatcaat cccgtttata tagttatttt tcaactcttg aatctttttc ggattaagaa
     9421 gatgcaaaat ctgaaaatta gaactcggct ctgaatagcg taatttagta taataattga
     9481 ttgtcagatt atcctgtttt gtctgaagtt cttccacctg gttcagaaac tttgtctcgc
     9541 tatctgaata gatatactca tttcctttaa ttttaggttc agggaaataa accttttctt
     9601 cctcaataag ttctgccaat ttacttgcag ggatatcctt ttgataaagg acaggattaa
     9661 tcttattgat tacagccggt tctatatctt caggtgcagc tgataaatta aagcttacat
     9721 cgccttctgc taaaatatga atcattgctt tacagccgaa taaataagct gcctgttgaa
     9781 tatcactact catcgttttt ctacctccgc taaggcaaag atagagttta ttggaattgc
     9841 cttgtttata gcagttgtaa actaggctgt atatcaaatt tctcattatc ctggcatctt
     9901 ctgcactgga tatatctttc aattggttta aaatgtgaaa ttgaagatct atatttaaat
     9961 gttgtgcttt aagccaatct tctactgcct gtttattgga tgtgaaacta tcatttgtgc
    10021 aaatcatact aatggtattt attggctcta actgattttt ctctcttgtt tctttcagcg
    10081 tctcaaatga cttatgatta taataaagag gatattgctc ataattgaaa aagccaacca
    10141 cttctgcaat taaatagctg gtttttccca ttgagatgat taactgactc atttacttat
    10201 gcttacctat aagcttaata ttttatccat tgaaaacata cacctatatt tgggttcgga
    10261 agcattaaac tgatagtggt aaacatcaat tggaacaaaa tgaccgatag caattcctaa
    10321 agccatacaa agagcaatgg gagctgtctg aaacaaatga attcgttgcc aatgattttt
    10381 ttctcttgct gcatttaagg cagaatttat ttcttgcaca aagggcagcc agttttggct
    10441 tgtttccatc actccctgat cttgtttggc tttaattatt aagaagttat ttacctgtaa
    10501 gttcttttgg caaaatgctt ttgcttctcc aatcggatta tggcttccca gataaataat
    10561 gaatcctaat tcgttttttt gaggatcatt tatttgcagt tcgctttgga tgtactgaaa
    10621 atcctctttt ttaaaggata catttttcag ttgtctggca ttttctttcc cgtataaagt
    10681 aaagacttct ttgtaagtag tattggaaaa ttccatttgc aatatactta ctgctctttt
    10741 aaacccaaag agggcaccaa tccctatttg taaagtgctt atttgtccag aataaaacca
    10801 caagaccttc ttgggcatta tcttatcttc caaagcattg aatttatctt ttacctgttc
    10861 tttcagtaat gtttgccagg catttttttc aaaaggaagg atgccattac caaaaatagc
    10921 taagttatta gttgttattc cgtaaaaatc ttccaagagc tcatagctga accagctgaa
    10981 gttttcttga actttttgtt ccagtgcttt ttgccagcgt ataagttcat tttcttcacc
    11041 ctgatattga ataatgggta tagggatagt ttctatattt aaccaggcat caagctggtc
    11101 acaatttttc accctataga ccagatgtgg gcaacagcgt tttttagctt caatttcatc
    11161 tgcttttatt atttctccat taggtaaaac gattccactg aaagccagat tggcaaatag
    11221 cgtatctttt ttagacagta gagttgccag aactgccagt tgccaggaat ttcctgtaat
    11281 atttttatca aagccgatta tacagttttg ttttcctaat tttgccagat agctgtagtt
    11341 ttcaaattta tctcccagaa tagctatagc ttttggttct gaactttcca gttgataaat
    11401 aaatgctctg gctaataagg catttcctga attatcaact acagggaaaa tacctatttt
    11461 cggttgttgc tgactaaggg agttaatttc agtttcagag gtttccaaaa gcttacttac
    11521 ctgctctaaa tattttccct tagcagaaag ccacatcaaa gagtttaact tcatattggg
    11581 gtttttcgct ttttccagta aggtataaac ccactgttca tcttcttggg gaactctatc
    11641 ctgttgtata tattgcagga ttatattttc tgcttcagaa gaactgttct ttaaatagtt
    11701 atctactcta tccctaaatc tgggcattaa tcttttatcc ttaatcctta atcactttaa
    11761 gcgttatatc taaaaaatcc tgggcttgtt ttggtaaaag agggataaaa ttagccagga
    11821 caatttgttt gggcaatttt cctttaaata ccactatgtc tccacagcga atttccgctt
    11881 tttggtcaat caaattatca tctggggcta aaattagatt atggcaatca tctttataca
    11941 aagtataata atcggcaaaa taggcatatt tatgtacctt aaataaatcg gttgagacaa
    12001 gcctaaacgc aaatttagcg atttccatat ctgctgcagc catttttaca ttgtttgccg
    12061 ggttgcttct atattgaatt tctatctgag gtacccaaat tttcggatcg ctatgtcggc
    12121 atcgcaatcc cagagtaatg ttatattcca taaggagaaa tttgttttca gggctttcca
    12181 ggtctgtatt tctgttttca tctacaagat gttcttttct tagttcctgt ttcaaactct
    12241 ctaaattact cggctgaaga taatccagtt ctattgcttc tttaatttct tcttctgtaa
    12301 gataaaagta attgcctgtt tctaaaaggt acttacccat atcggtttta aacacaatat
    12361 caaattcgct ggcaaaaaca gtgaaggggc ttgttttata agccaaatac tcattatcaa
    12421 tagttttatt cactacgata cagtcaatta ggtattcatc tgtttccagc acaatcattt
    12481 ttccaagtaa ttgttcagtg tgtggttgga attcgggaaa agtaaactcc acatcttcag
    12541 gtagaacaaa atcaatatcc tccagttcta taataggaac ttgtgtctct tttgtaatac
    12601 cctccagata tgtctggtat aaaagttctt tatctttttc attcattata aactccttag
    12661 gtttaatagc ccttctcttc tttatatgtt ggtaagggag gacagttctg acatattttt
    12721 ttcaagaatt ccttaataaa aagtcgccct acattttcat caaaattgtt gttttctaag
    12781 gtcagtttaa gcttcgttct aattctctca tgcaatttat agatgttatc tctgctttca
    12841 ttttttaaac gattactgcc gctttcacaa ttgttttcta ggtcatggca taaaacatta
    12901 atttctctta ttttccattc ttcttttatt atttccgcta atagaatagc ttgatgtctg
    12961 gtaactattt catattgaat ataactaaga tcttgatatt tactgatgtt atagttacca
    13021 tagtattgtt ctatcattat gctttcaatt ttattactta ctatatcatt gatgaattct
    13081 tccacgcagt gcattgtaac tatttcgcct gtcttaaata gatatttacc aaagtcctct
    13141 tcactcttta attggttttt tttctcttcc agttttttaa gattaggagt tagtttcata
    13201 attaatcttc ctatcaagca aatatgtgtg tcttcagtat tataaccttc cttataagcc
    13261 aagttcttaa gcatagaatg ttggcttggt cttttgtgta ggaacatctg gattagtgca
    13321 tagacctgat agaaaactat cttattacta cttaatagat ttggctcaca tagcacagac
    13381 cataacgcca tttccaaaga aagtatatct ttaccgtcct ccgaattgtt ataccaattt
    13441 ttataacctt gaattaaaga agtatcctta ttcataataa cttccttatt ttcttttttt
    13501 ttacattatg aaaaagatgg gattttttgt caatcaaaac agtttgctat aatattacaa
    13561 actcaccaaa aaaaccaaca ctacaaatta tccactaatg cactttcccc tgcttaatat
    13621 ctatggtgga gatgtggctg tgcttcttgg gtattttccc actattgcca cggtagctct
    13681 actacagcta atcaggcaag atcaattcct atttccgaaa tggcaaagag aaaagacaat
    13741 aggaaaggga tgaacaggat tattttagct aagggcttag ggctgagggc tgagggctga
    13801 gggcagggat gaacaggatt attttagcta agggcagagg gctgagggct gagggctgag
    13861 ggctgagggc agggatgaac aggattattt tagctaaggg cagagggctg agggctgagg
    13921 gctgagggct gagggcaggg atgaacagga tttatggtat tattaggatt tcgaatagaa
    13981 gttccaaggg gacgatagat tctaacgacg ggatttaacc cggcgcaaga atgtcggaag
    14041 gttgatgacc ccatcaactc acaacaaaca aactatcaag ctattacaga atatagcaga
    14101 aactacacac ttatcaactt cccctctttt atatcctgct taactttagg ttcaaacgca
    14161 tctctattcg aaaagtgcat ccttttaata ggcttttttg ttgatttctt tctccatatt
    14221 tttagtccac ctttttcagg atgattcata ctatgccctc agttctttgc ttctgagcct
    14281 ccaataaatt tgtccaattt tctacagttc cctccatata tctataatag atttaattat
    14341 atgctatgaa taaatataag cggaaaaccg caaaaaacaa aggagaaaaa atgatgcaag
    14401 aaaaagtaac tgaactcggt tctcaagcta tcctactgcc ggaaaatgaa tttcattctt
    14461 ttcgtctgca gtttaatagt ttatttatca aagaagagct aaatactgct cataatctcg
    14521 ctttaaaaac gctttcgtca gaaaatctga atagtgatga gctggtagaa ttagctcgtt
    14581 gttttcagct cctaggagat aaagataata ctcttacctg tttggaaaaa gccattcaaa
    14641 ttgatgatca aaacaaaaaa gccaaagtat taaaactgga attgcttgac tccttggagc
    14701 aaaaaggaca atacttggat tttttacagc attgtttaca caacgatcct caagaaaagc
    14761 agtattattt gcttttgcat actttttata ctgaaaatgg tcagaatgaa cttgctgaaa
    14821 atgtctctgc tcttgcttta agcaatggaa taaatctggt tttacctaat gtggaaattg
    14881 aaattacggg ggatgatttt cctcctgatc cagttgcaat tgaagatccg attttgttga
    14941 gcaattatct cactctcttt gctggtaggg aaaactgtta tgcccgacaa tgggtttccg
    15001 ataaaggtaa aacaggttac actccagtta tagaaccctt aaatcctgtc ttgatccgta
    15061 atcatttaca aggtattcaa accttgggag tatatcaatt aactttgaaa aatcaggtaa
    15121 aatggattgt ttttgacata gatattatta atgattatct tgatgatata catgatccgc
    15181 attttcgcga atgggtagat aacggttttc tgcaagttct aaataatttt gataatatct
    15241 tgcaaacctt tcagctgcga gctgtttatg aatacagtgg ttataagggc tatcatatct
    15301 ggctattctt acaggaatat acaagtgctg ctattgctag aacatttgct ttgaaacttg
    15361 ctacccaaat tgatattagt tcctttcctt tccaaattga agtctttcca aagcagaccc
    15421 gcacttcaac taataatttt ggtaatctta taaagctccc gggtggagtt catcgttttt
    15481 ctggtttgaa aagtactttt tttactttga cagatggtgc tttagaaccg cttcctctgt
    15541 cttctttgtt aaaaaaacct ccgttaattt ctccgagtga ttttttgtct gccctctgtt
    15601 ctcttcagcc agatttttcg tgtaatactt tggattctag ccgggaaaat tatcaaactg
    15661 aaaatgtaaa tatatccatt atccctgctg aaccatctcc ggaacttgat cctcaatggc
    15721 tttggctaaa acagcgttgt tctgctttgg cttttatttg tcagaaaatt gaagcccatc
    15781 atcaattgaa tgatgctcaa aaaaaagtcc tcattcattg tgtgggtcat ctggatagag
    15841 gacctgaaat tgttaatttt tatttaagac aatgcaaccc ttgtgatcct tccgatttct
    15901 taaaatctac tctaaaaggg aatgttatta gttgcaataa aatacgcagt tatttagctt
    15961 ctgaaaatga tagctttgat tgtgattgcg atttcagcgg aaaaatcttc agctatgaac
    16021 atcctcttat tcatctggag gattatgttc tgtcttcttc ccctttttct ggaaacaacg
    16081 aactcctcct gaaagatact atttctcgtt atttggatat aaaacgcaga aataatgaac
    16141 tttccgaaac tctgcaacac ttggaaaaac atattttaga actttttgaa caaattggag
    16201 tgagtgaatt caaaactccc tacggtgttc ttaaaaaaga taataccgat aataatgtaa
    16261 aactaattct ggaattggta taggagctat taatgaatga aaatagagat gctgttaata
    16321 atgcctggaa tcttattaaa caaggcaatt atagagatgc tttagctgta ttggaagcat
    16381 attctcctaa tgatttatct tcccattatt ttcttgcctc cctgttgttg gaatgttata
    16441 ataagctgga aatgtataat aaagctatta actgcgggga aaacctatgc actcaaaaaa
    16501 attgcagtcc atcaattaaa caaaactatg cctggagttt gtattttgct tattttaaaa
    16561 aaaacgaggt tcttccttta gaagaggcaa taccacttat agaaagaatc gcctctttaa
    16621 cagattgtac taaaaaagta aatcctctgg tgctaactat attttcctat cttacaaaaa
    16681 ataaacaact tagtcctgaa attattattc agcttttgga tatgctgaag tttgatctct
    16741 tagatgataa agcagttaat ggaaaaaaca aataccaaac ctttccttcg cagaaagaga
    16801 aatatatttg ttactatacc aaagctcttt ttctggaaaa gaactactct cgttgtattg
    16861 cctgttgcaa aagagctttg gaattaagca ctaactatga taattttaag aataaaggaa
    16921 atccttcgga agatgcttct ctttgcctac ccgatgttaa aaatgctctt tttatttctc
    16981 ctgcaaacct tatttggatt aaaagatatt tggctttgtc tcttttttat ctgggagaat
    17041 atgaacaagc atatcttctt atgcaggaaa taattaaaca aaaaaaggac tggttcctct
    17101 attatgaatt aagccaaata actcaaaagc taaataaaat tgaggatgct cttcgttatg
    17161 ctgccattgc tgcttattcg cttggagata tagaaatgaa aatccatctc tgggaatacc
    17221 tatatcagct tttactgcaa accaaaaact ttcctcaggc agttaagatt ctttctcttt
    17281 ctgctgcagt ccgtgcccaa aaaggttgga aaatttctcc ttctctaaaa caagaaatag
    17341 ataaacataa tctccagcca gaaaatcttc cctcctataa aaatatcttt actgagctaa
    17401 aaccctggtt tatagaaatg tcctattcag gttcagaagt tcttacaggt tatatatcta
    17461 atatattggc tagtaacaaa gcaggtttta taactaccgg aaagaaatat tattatttca
    17521 aaaccagttc ctgcctgttt gaaccggaat atattgtgcc caatactaag gtctctttta
    17581 atactgaaaa aagctttgat cctaaaaaac agaaagattc cattatagcc gttaatatca
    17641 aaaggatata attatgcccg ttgtctatat tgccgatcag ggttctcatc tgtgcaaaag
    17701 aggtgatcgc ctctatgttt atagaggaac tcagttattg cgctggtttc atactaagga
    17761 tattatccaa ttgattctgg tggggaatat tggtctaact tcccaggtag ttacttattt
    17821 gctaaaatat cgcattgata ctgttttcct ttcctattac ggaaaattta agggacgcct
    17881 cgttggtgaa ttcggtaaaa atgttctgct tcgggtcaat cagtttcaat atcttagtga
    17941 tctggaaaac agaactcttc tggcaaattt aataattcgc ggaaaaattg ccaattccct
    18001 ttttcatcta gccaaaagaa aaaaacggaa taaggaggaa tctattactg tagcttatat
    18061 taaaaataaa gcactattag cccaattaaa ttcccaaatt tatccaaaag atatcttgct
    18121 tggctatgaa ggtattgctg ccaaaaatta ttttgctgcc tttcctgctc ttattgccaa
    18181 taacgatttc cctttttcag gtaggaataa aagaccacca aaggatgagg ttaatgctat
    18241 gcttagtttg tcctatactt ttctgatgaa tcaggttatg tgctctgctt atatctgtgg
    18301 cttagatccc tattatggtg ctcttcacga tttggattat ggtaggcagt cattagtttt
    18361 ggatgttatg gaagaattca gacccctaat tgataatatg gttatttcac ttatcaacag
    18421 aaaagaaatt cgcctcgaac attttctcta taatactcta cctaatgatg aagatcctga
    18481 ggaagaaaac tccgttttac ctgtttccct tactaaagat ggaatgaaaa ttattatcac
    18541 tgccttcagt aaacttataa acagcaaatt ccctttgcag gatttacctg gtgaatggtc
    18601 tatgaaagat attattactc ttcaaatccg aaaacttgct gctcatttcg aaaataaaaa
    18661 ggagtattta cctttcttat ggatgtaatt tatgtatccg agagacatct ttactcagcc
    18721 ctcaaccctc taccttttgc attaaacaag gagtactttg tatgtcccta aacaaacact
    18781 tatacatcgt tgcctttgat atcgtctccg ataaaatccg ttaccgttgt gtaaaaatcc
    18841 ttctaagtta cgggcaacga gtccaaaaaa gcgtttttga atgcattctg aatgataagc
    18901 aatttttaga aatgaaatct aaacttgata acacaattaa ccttgaagtc gatagcgttc
    18961 gttattattt actctgtgca aattgtgaaa aaaatatatc cgtttccgga acaggtcttt
    19021 actcttacga tgaagaactc ataatcattt gatgaaaaac agcaagatga tctactgttc
    19081 tatacccttt tttaacactc cttctttctc aatctttcag ataaattata tccacatcgt
    19141 aaaaaaaaca atatctcatt ttatccttga ctttttgtac atcagaaata taaatacagt
    19201 actgagaatt aagagtttat tatttcaaaa taaattgcac cgttcagtat gccaaaagga
    19261 gctaatatta tgaggtttta ccgaatttta ttcaaaccct taaaccaaat taataggaca
    19321 gtgatctggg atttttgtca gtatctctct aacctatact gtaataatat aattacactt
    19381 tttcccacca ttttcgctgt accttttgca actccttgtt atttatatga ttatataaat
    19441 ataaaattta taccccctac cctctatgcc ctaaatggca tccatagctt ttttacctgt
    19501 tctaaatctt ttactattca aacctctttt actgtgctat agaaattgaa ttgacccgtt
    19561 agaggggatt gagacttaat ctctttctca tcctctggag agaaagaaac cttttataga
    19621 aattgaattg acccgttaga ggggattgag acttaatttg cctccaagtg ctttaaatac
    19681 cctgtagc
//
LOCUS       DAOSTH010000023        19419 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_2983_length_19419_cov_16.205639, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000023 DAOSTH010000000
VERSION     DAOSTH010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 19419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19419
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2983_length_19419_cov_16.205639"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..515)
                     /locus_tag="PLB65_04500"
     CDS             complement(48..515)
                     /locus_tag="PLB65_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77822.1"
                     /translation="MVEKLKELYPEKSKCWKPGVTNAQENNRTFNIICDKNIKKTIVK
                     VWIDGCLIPDQDKKKCDYMFIIPEGKVLILVECKGNDVKKAIEQIRSTIQILKPLVDL
                     SSQKYAFIIPTKVAPAIQSNIQIVMKKFMREYGIELIVKSHHCDFNLANKKVN"
     gene            complement(522..1781)
                     /locus_tag="PLB65_04505"
     CDS             complement(522..1781)
                     /locus_tag="PLB65_04505"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ77823.1"
                     /translation="MNTLTIKNFGPIKDISLNLKKVNLLIGTQGSGKSTIAKILSIMN
                     DPELVMADTLDKFLVDYNINYDINENTFMEYKTPEFNVTIESNQIKHSLKKTLNDEEL
                     RKFVLSKLKISINDINKWFNTPKESLKSESNEIEQDLINKGFEITSDKIEDTQEYIKF
                     VFNYPIIQLASTLFLESCIYIPSERNFFALLAENIFGLLAENIISSGKKDMTVLPKCI
                     LKFGRYFEQAIKSIKKDDAVDIPFIEELKFIRREGRNEIIYKGVSNSLTQGSSGFQSS
                     IPLAIVCLQNKNSDRSLFIIEEPEQNLYPVTQYNMVKFLAENCLNQNNKLLITTHSPY
                     ILTSFVNLIQAHSSGAIHPKLTAKLIPKTQWIDFNDVSAYFIDKGSAKDILDYEEKTI
                     FAEEIDAASSDIANEYNQLLEIAYRNR"
     gene            complement(1844..2782)
                     /locus_tag="PLB65_04510"
     CDS             complement(1844..2782)
                     /locus_tag="PLB65_04510"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HOQ77824.1"
                     /translation="MNLAGIQNFRKSRKGKVILYFLKLALSVLILWQIFKKVDLFSAL
                     TDITSLPAYIILLLIAISFLRHTLQFHNWYYSLKLHSKFREKKKSVVSSYFVGQTLRF
                     AVPGGPASFAKIFYVPNNSVLASFWSSIAEKSVMTWVTWTFAIIAGIFYYSKFPLWIW
                     IIVLLVLLSFPFLIKLFLGLSKNYATMKPLYAIQIPKVIAVQTFSTILCYVQYWLILN
                     CLLPLSFWQTFIRMALTQFSNTIPITISGLGLREGFAIHFLAGAGFSAEQAVSTTLTL
                     FFIHDVLPALIGIFFLLQGKKNKSRQAGQGKDIDEI"
     gene            complement(2786..4243)
                     /locus_tag="PLB65_04515"
     CDS             complement(2786..4243)
                     /locus_tag="PLB65_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOQ77825.1"
                     /translation="MNELNANLSNLAEVFSELFAPENQSAAQTYFLKELSLKMHSFYQ
                     GKMQTLPKAGIWGFNWFNVWYTPGVSSVSTTIRDNPLKSYELSNRGNLVAVVSDSTRV
                     LGDGDCGIYGGLGVMEGKAMLMKYLGACDAISLCIDSRNEEGKPQAQKIIDFVKMLQP
                     SVGAVNLEDISQPNCYLVLDELRQSCSIPVWHDDAQGTACVTLAGLLNALKLADKDIK
                     KIKCVLFGAGASNTTIASFLMQSGLNPEKLIMFDSKGSLHPKRYDLRDNPAKFKQWKI
                     AQISNPDCQDGIENALTDADVLIALSTPGPDTIKPQWISKMAKKAIVFTCANPVPEIY
                     PYDAKQAGAFIAATGRGDFPNQINNSLGFPGILKGALTVQASKITDNMAIKAAHSLAN
                     FAEKKGINPEYIIPQMDEEDVFAYVSADVATQAIIDNVARKELSWNEVFNIVQREIRE
                     TRSLCLEMMNSGYLKPPDKAIIDGTLQETIMRFGN"
     gene            complement(4534..5430)
                     /locus_tag="PLB65_04520"
     CDS             complement(4534..5430)
                     /locus_tag="PLB65_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HOQ77826.1"
                     /translation="MDMKTFGYLYYKTRGYAHDDILKNAEITEEEYEFFEKNLQDVLK
                     EIETLRPVAENIGQDFVRLTRYIYERESDQTLGVQRPDAVKARVGEIVSLPAIGTLTM
                     PDMPLAKAIEQRRSRREYSQEALKQEELSFLLWASSWARDFRSTERMELTFRNVPSAG
                     SRHPIETFLDIRRVEGIKPGLYYYHPIKHCLILYDNSEQIAAKIHEGCMFQEMISNAA
                     VNFILTAVPYRTVWRYGQRGYRYLYLDAGHIGQNIHLAAEAIDAGACMIGAFLDEAMN
                     EALGLDGIEEFVIYISSVGKKP"
     gene            complement(5447..6502)
                     /locus_tag="PLB65_04525"
     CDS             complement(5447..6502)
                     /locus_tag="PLB65_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HOQ77827.1"
                     /translation="MIALIMAGGVGTRFWPKSRKNFPKQFLKVSGELSLLQMTVERLL
                     PQFSFTDIYIVTAQNQVELIKEQLPELPEQNIIIEPFGMNTAPCIALSVMYLKNFYPE
                     QELMFVLPADHIITDKENFLQSLLPAEEQAKKGYLVTFGILPTYPATGYGYIEAGEEI
                     APEVRKVLNFKEKPDKATAEQFLAKGSYYWNSGMFCWSLKSISTAFTNFLPEVDNITK
                     AIANKWQENGLQTDISSLYAKMPRIPIDIGIMEKADFRGVVPVSYDWSDVGSWKALSD
                     LVNKDEDGNYFSAEGKAISATNNYIDTNKFTAIIGVNDLCLIETADAILLCNKEKAEE
                     VKKIVEHLQQVNREDLL"
     gene            complement(6499..7119)
                     /locus_tag="PLB65_04530"
     CDS             complement(6499..7119)
                     /locus_tag="PLB65_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HOQ77828.1"
                     /translation="MKIAKITPLILFLSCISLLGITSEALHQKIANTYSSFSSFQANL
                     KQENYFAQLNKSITYQGNIYFTKGRMVMRFTKPSFQRLMISGGIVELYDSESKTVFRS
                     KMRPEFGKMNPVEILQLYWKKSSVNVQSSKGDLANVTLKPYSDPIIVSISAVVDTKTG
                     IIQSLSWTNKNNDKVTYNFSNIKTNTKIPASIWQFTYPKDVQVVEQ"
     gene            complement(7106..9352)
                     /locus_tag="PLB65_04535"
     CDS             complement(7106..9352)
                     /locus_tag="PLB65_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HOQ77829.1"
                     /translation="MANNPKHKTKSHKASQKMSTAKKRTISGIICLLSILVIIALNMG
                     YNSIKNDLESLKAAGSIFSWFQAGNSTTNNPVGIFGIVFGYVFIYLLGFWFSLIGFIL
                     IALISFQYFLAPEAEKGRQKAYLLILVMFLLQVLLVSGQQEYIHSIIPLFVYRILAAV
                     FSGVGARIILIGAMILTVIMLIEYERLKAWISAAAENLQQKKETKIKAPKPTITANNI
                     NPEQTVAEETIPNIQNHTILNPSIDSTPKEFPKPQKENKTKRVFSEGTDKEYQLPAID
                     EFLESPIKLSDREHKEIENQIITTSQILKSKLAEFGIEAEVKNVNIGPIITQYELEPA
                     KGIKVSRFTSLADDLALAIKAKSIRVQAPIPGRGLIGIEIPNLARDMIYLKDLLLSEQ
                     MKQTSSKLAFGLGKDIAGKPIVADLAKMPHLLIAGATGSGKSVCINTIIMSLIMRTKP
                     DDLRLILIDPKRVELAAYNELPHLIGQVVTDADTALETFIWAVREMERRYEILQDARV
                     RDIIGYNEKCEEDEDLEPLPYIVIIVDEFADLIMTSGKDIELPITRLAQMSRAVGMHL
                     ILATQRPSIKVITGIIKANFPARIAFQVSSRVDSRVILDMIGAERLLGNGDMLFLPPG
                     KALPERIHGAFVSDAEIARVCSFLATQPKPTQDFSIVVEDKESLGFFDYDDELFPEAA
                     RVVVSSGTASVSMLQRHFKIGYARAGRLIDLLERARIVGPHLGSKSRDVLATREDLIR
                     IGVIDENR"
     gene            complement(9374..10450)
                     /locus_tag="PLB65_04540"
     CDS             complement(9374..10450)
                     /locus_tag="PLB65_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HOQ77830.1"
                     /translation="MKAAIIGLPKTGKTTIFNALTRSEHNTDKYAPASLEANIGVVQV
                     ADERIDKLSQLYQPKKTIYATIEYHDYPGIFYKESETSDTALLSDLKTAEAFILVLRG
                     FIDEELEGLYPLGSPETQFQHFQDEMLLADLIMAEKRLEKIELGYKRGVKTPAIQIEE
                     KALRKIVDCLQNNLPLTNLELSSEEEKAIRGFQFLCFKPMMILINCNEDNLTAWDDTL
                     NTFREKGFPAHSIAGKFEEELSKLDNEEAQMFMEDMGISDSIRDRLTHWTYELMGYIS
                     FFTVGPDEVRAWTIEKGSNAVTAAGKIHSDLARGFIRAECFQYEDIINLGSEKALKEK
                     GLFRLEGKDYIVKDGDILSIRFSV"
     gene            complement(10447..11238)
                     /locus_tag="PLB65_04545"
     CDS             complement(10447..11238)
                     /locus_tag="PLB65_04545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOQ77831.1"
                     /translation="MKSSSPNSTEPSSKTVSRAGKTIRLNRYLADCGLGSRRKVEEFI
                     TSGRISVNGVIVKDLSTQIFSGKDTVTLDGKIVQPELKKVYLILNKPRGYVVTRSDEL
                     GRKTVYSLLPQQAKELSYAGRLDKDSEGLLLFTNDTILINMLTHPKNKVEKVYRVDIN
                     RSLTNRELNILRNGVEIEGGKTLPAGVFVKAQNIEGMSLKIVISEGRKRQIRQMIEAV
                     DAQVTYLKRLQFGPIKLKKLPVGSWRFLDSAEIQSLLYIKKGKNR"
     gene            complement(11177..11677)
                     /locus_tag="PLB65_04550"
     CDS             complement(11177..11677)
                     /locus_tag="PLB65_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptE family protein"
                     /protein_id="HOQ77832.1"
                     /translation="MVKKFIYPVLLLLILSSCFYSVYSNAYPHLRKIRILAFENRTSE
                     YGLGDKLLNYLNREIRNDGRLKIVTEDPDCTLEGAILSFSESVYSYDTANQIQDYQLT
                     MVVSVTFTDLINNTVLYENNALTVTELYAVAEGGTAKFKTKEEAVEELIAKLYRTVLQ
                     NSIEGW"
     gene            11773..12447
                     /locus_tag="PLB65_04555"
     CDS             11773..12447
                     /locus_tag="PLB65_04555"
                     /inference="COORDINATES: protein motif:HMM:NF012579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOQ77833.1"
                     /translation="MAKKFISKAEAAKKLKVSERVIQEMIATNVFESKLVGKNIKIDE
                     DSLNEWLENLNEIDEKMLALKRVICHFEEYMRPENIFLDFTAENKYDAIRILSEKAKD
                     LKLVRDARWLYEVVVAREELVSTAIGNGVALLHPRHLHPSKIKTPSILFGRSSEPVDF
                     DAPDNKPVNIFFMLLLHNDKQHLFSLSYISRLVMDQDVLKRLTEAADATEIHQLLTVI
                     PEQTNK"
     gene            12444..12896
                     /gene="purE"
                     /locus_tag="PLB65_04560"
     CDS             12444..12896
                     /gene="purE"
                     /locus_tag="PLB65_04560"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HOQ77834.1"
                     /translation="MKPIIRIILGSKSDLDKIAEAKDVLEQFKVPYDLFISSAHRNPD
                     KTIALAQNAKSEGIEVIIAGAGLAAHLPGIIAANTTLPVIGIPFAAGALNGLDSLFSI
                     VQMPPGVPVASMAINGAKNAALFAIQILALKDKELEAKFATYKKDLSA"
     gene            12939..13688
                     /gene="gpmA"
                     /locus_tag="PLB65_04565"
     CDS             12939..13688
                     /gene="gpmA"
                     /locus_tag="PLB65_04565"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HOQ77835.1"
                     /translation="MYKIVLLRHGESIWNKENLFTGWTDVDLSEKGIVEAQNAGKRLK
                     EEGFVFDEAWTSVLKRAIRTLWIVLDEMDQMYIPIHNDWRLNERHYGALQGLNKAETA
                     IKYGEEQVLLWRRSYDTPPPPLEKNDPRYPGFDPRYANLREDQIPLCESLKDTVKRTM
                     PFWNEIIMPRLAAGRKLIVSAHGNSLRAIVKTLNNISDADIVSVNIPTGIPLIYEFDD
                     NLRPKKNYYLADKAELEKAQQAVADQGKVKK"
     gene            complement(13841..14338)
                     /locus_tag="PLB65_04570"
     CDS             complement(13841..14338)
                     /locus_tag="PLB65_04570"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOQ77836.1"
                     /translation="MSIVTKGGDSGKTSLYSGERVFKDDLRVEAYGTLDELNAWISEC
                     RHHLPNSVYKDALREIQQNLSRCMGQLATKNGSFPDPISQNDVDNITEYVYLLEKNLH
                     FDGFVIPGGTPVSAKLDICRTVARRAERRIITLSHKEHIAPELLSYVNRLSDFLFALA
                     RFLET"
     gene            complement(14343..14714)
                     /locus_tag="PLB65_04575"
     CDS             complement(14343..14714)
                     /locus_tag="PLB65_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOQ77837.1"
                     /translation="MQSVMTHRAPAAIGPYSQAILMNNTLFVSGQLGIDPGTSSIPEG
                     FEEQANLVFNHIKSILDAAGMGMSNIVKVTVFLKDLDDFTLLNDIYTRYFSAPYPARE
                     VVQVSRLPKGGKIEISVIAMR"
     gene            14986..16266
                     /locus_tag="PLB65_04580"
     CDS             14986..16266
                     /locus_tag="PLB65_04580"
                     /inference="COORDINATES: protein motif:HMM:NF024785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOQ77838.1"
                     /translation="MVQEPQDFHEFMPNEVWKWKILEEKIEKVLSLHDFQEIRLSILQ
                     DSQILENGIMALVQGKEADSAMKGVLSLANPEDKLSLLSLRPEGTISVLHNTARIANP
                     GDIHRFYYSGPMFRKVDNNQQMEFYQLGVELLGSDSILSENEVIDLGIKLCKTLGLND
                     VRLELNSYGCSNCRRPFFNDLHKYLEEHKSEYCLSCYNELSANPFSQVHCKDPKCKKS
                     LAEGPLIHNYLCKSCKADFRKVKSIQANLGNQYKVNPLLNKNFAYYNKTVFDFIAQNA
                     GKETVIGGGGRYDYLSSLITGKNIPAVGFYLNLDTIFDIMESQGLFATKDPEFVVYIC
                     AESPNLETMTLQIAQELHSQQVKTIISPECKQIKEEIAIAKQNKCPIMIILREDNLRE
                     GKVLIRNLVKEEQNYISLKDTLSTILLARKSMQS"
     gene            complement(16823..19399)
                     /gene="clpB"
                     /locus_tag="PLB65_04585"
     CDS             complement(16823..19399)
                     /gene="clpB"
                     /locus_tag="PLB65_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOQ77839.1"
                     /translation="MNTQRFTIKSQELLADMQDLASSYGHQEIKDLHLLQAMLSQEEG
                     LILPALQKLEVSVPKVKSEVENALNKLPKISGSSQQYLSQEVLDILHQAEKEADQFQD
                     DYISTEHLLMALLVKGKEAANILKSEGLQADKLLLALKELRGNQRVTDQSPEAKYQAL
                     EKYTRNLTRLARMEKLDPVIGRDEEIRRCIQILTRRRKNNPVLIGEPGVGKTAIVEGL
                     ARRIVARDVPENLMDKDLLELDMAAIIAGAKFRGEFEERLKAVLSEVEKSEGRIILFI
                     DELHTVVGAGAAEGSVDASNMLKPALARGNLHCIGATTLTEYRKYIEKDAALERRFQP
                     VLVTEPTVEDTISILRGIRDKYEVHHGVQISDNALVAAAQLSERYIADRFLPDKAIDL
                     IDEACALLRMEIDSLPVELDESERRLRQLEIERLSLAKENDPLSQERLEKIKEEIAEL
                     NEENRALRLRWEKEKQVLEQIRALKTEIDTLKGEAEKAEREGNYEKTAQLRYGTIAAK
                     EKELQKLLQNETLTKKDRLLKEIVDEELVAEIVSKWTGIPVSKLAESEMQKLLELENV
                     LAQRVVGQEEGIKALANAIRRSRSGLSDENRPIGSFIFLGPTGVGKTELAKTLASYLF
                     DTEKALIRLDMSEFMEKHSVARLIGAPPGYVGYEEGGYLTEAVRRRPYSVLLFDEIEK
                     AHPDVFNVLLQILDDGRLTDGKGRTVVFKHCVIIMTSNIGSQEIYEASDKELERLKPQ
                     LMNILQQYFRPEFLNRVDDIILFHRLDKDHIKKIVKLQLNLLAERVANRNLQLDFSEN
                     LIDHLAEAGYDPAFGARPLKRLIQKEIEDTLARHILAGQFTTGKSIMLDWNPQEGLII
                     TQ"
BASE COUNT         5930 a         3834 c         3488 g         6167 t
ORIGIN      
        1 tgtgtattcc ttgcgagata gcaataatta tagatttact atagcgatca attgactttc
       61 ttattagcta agttaaaatc acaatggtga ctcttaacta ttaactcaat cccatactct
      121 ctcataaatt ttttcattac gatctgaata ttggattgga tggctggtgc taccttagta
      181 ggaataataa atgcatattt ttgggagctt aaatcaacca atggtttcaa tatctggata
      241 gttgaacgga tttgttctat tgccttcttt acatcatttc ctttgcattc aaccaggatt
      301 aatactttgc cttctgggat gatgaacata tagtcacatt tttttttatc ttggtctggt
      361 attaaacaac catcaatcca tactttgact atagttttct taatattctt gtcacaaatg
      421 atattaaatg ttctgttatt ttcttgggca tttgttactc ctggtttcca acacttactt
      481 ttttctgggt aaagttcttt taatttttcc accataattt tttacctgtt gcggtaggca
      541 atttccagca actgattata ttcattcgca atatcagaag aggcagcatc aatttcttcc
      601 gcgaagattg ttttttcttc gtaatccaag atatcttttg ccgatccttt atcaatgaag
      661 taggcgctaa catcattaaa atctatccat tgagttttgg gaatgagttt agccgtaagt
      721 tttggatgta tcgcaccaga actatgagct tgaatcagat ttacgaaaga agttaaaata
      781 taaggactat gagtcgtgat caataattta ttattttgat taaggcagtt ttcagcaaga
      841 aatttaacca tattgtattg ggtaacggga tagagatttt gttccggttc ttcaattata
      901 aataatgatc tatcgctatt tttattctgt aaacagacaa tagccaaagg aattgaggat
      961 tgaaaaccac ttgagccctg ggttaaacta ttggaaacac ctttataaat gatttcattt
     1021 cttccttccc ttctaataaa ctttagctcc tcaatgaaag gaatatctac agcatcgtct
     1081 ttttttatgg actttatggc ttgttcaaaa tacctaccga acttcaaaat acactttggc
     1141 aatacagtca tatctttttt tcctgagcta ataatgtttt cagcaagtaa gccaaaaatg
     1201 ttttcagcaa gtaaggcaaa aaagtttctt tcagatggta tatatataca ggattcaaga
     1261 aataatgtac ttgctaattg aattataggg taattaaaaa caaattttat atattcttgt
     1321 gtatcttcta ttttatctga agttatttca aatcctttat taattaaatc ttgctcaatc
     1381 tcattactct ctgattttaa tgattctttc ggtgtattaa accatttatt aatatcatta
     1441 atactaatct ttagtttaga gagaacaaat tttcttaatt cttcatcgtt taatgttttt
     1501 ttaagagaat gtttaatttg gttgctctca atagtaacat taaattcggg tgttttatat
     1561 tccataaagg tattttcgtt gatgtcatag tttatgttat aatccaccaa gaacttatct
     1621 aaagtatctg ccattactaa ttcaggatca ttcataatgg aaagaatttt ggcaatagtg
     1681 ctttttccac tgccctgtgt tccaattagt aagttcactt tttttaagtt aagtgatata
     1741 tctttaattg gaccaaaatt ttttatggtg agagtattca tttcattctc ctctatcaac
     1801 gcaaagtaat atcattttcg ttttttcctc ttataaagga aggttatatt tcgtcaatat
     1861 cttttccttg acctgcttgc cgggatttgt ttttttttcc ttgcagcaag aagaaaatac
     1921 caattaaggc aggcaaaaca tcatgaataa aaaacaaagt gagagtagtg gacacagctt
     1981 gttcagcaga aaaaccagct cctgccaaaa aatggatagc aaatccctcc cgcaatccca
     2041 aaccggaaat agtaataggg atagtattag aaaattgcgt aagtgccatt ctgataaagg
     2101 tctgccagaa actgaggggc aaaaggcagt tcagaatcag ccaatattgc acataacaga
     2161 ggatagtgct aaaagtctgc actgctataa cctttggaat ttgaatagca taaagaggtt
     2221 tcattgtagc gtaattttta cttaatccca aaaatagttt aatcagaaaa ggaaaagata
     2281 gcaacaccaa cagcacgatt atccaaatcc acaaaggaaa cttggaatag taaaaaatcc
     2341 cagcaatgat agcaaatgtc caagttaccc aagtcattac acttttttcg gcaatagaac
     2401 tccaaaaaga ggcaagcaca ctattattag gcacataaaa aattttagca aagctagcag
     2461 gaccacccgg aacggcaaaa cgcaaagttt ggccaacaaa gtaagaactt acaactgatt
     2521 tcttcttttc tcgaaattta gagtgtaact tcaaagaata ataccagtta tgaaattgta
     2581 aagtatgccg aagaaagctt atggcaatca gcagtaaaat tatataagca ggcaaggaag
     2641 taatatctgt taaggcagag aataaatcca cctttttaaa tatctgccac agaatcagaa
     2701 cgcttaaggc aagcttgaga aaatataaaa tcacttttcc ctttctgctt ttgcggaaat
     2761 tttggatgcc tgccaaattc attatttagt tcccaaagcg cataattgtt tcttgcaagg
     2821 tgccatcaat aatagctttg tccggtggct taagataacc gctgttcatc atttccaaac
     2881 agagggagcg agtttccctt atttcccttt gcacgatatt aaaaacctca ttccagctaa
     2941 gttctttcct ggcaacatta tctattattg cctgtgtagc cacatcggca gatacatagg
     3001 caaaaacatc ttcctcatcc atttggggaa taatatactc cggatttatc cctttctttt
     3061 cagcaaagtt tgctaaagaa tgggctgctt taattgccat attgtctgta attttgcttg
     3121 cttgaacggt taaagcgcct ttcaaaatcc caggaaaacc gagggaatta tttatctgat
     3181 tgggaaaatc gcctctaccc gttgccgcaa taaaagcacc tgcttgttta gcatcgtaag
     3241 gataaatttc gggcaccgga ttggcacagg taaagacaat ggcttttttt gccatcttac
     3301 ttatccactg tggctttatc gtatccggac cgggggtaga aagcgcaatt aaaacatcgg
     3361 catccgtcag cgcattttct ataccatctt ggcagtcagg attgctgatt tgggctattt
     3421 tccactgttt gaatttagct ggattgtctc ttaaatcgta tcttttcgga tgtaacgaac
     3481 ccttactgtc aaacataatc agcttttctg ggtttaatcc gctctgcata aggaaactgg
     3541 caattgttgt attagacgct ccagccccga aaagcacgca tttaattttc ttaatatcct
     3601 tatctgccaa ttttagagca ttcagtaaac cagccagagt aacgcaagca gttccctgtg
     3661 catcatcatg ccaaacggga attgaacagg actgccttag ttcatccaaa accaaataac
     3721 aattcggctg cgaaatatct tccaaattca cagccccaac ggagggctgc agcattttta
     3781 caaaatcaat gattttctgg gcttgcggtt ttccctcttc atttctgcta tctatacaca
     3841 gggaaatagc atcgcaggca cccaaatatt tcatcagcat tgcctttcct tccatcactc
     3901 ccaagccgcc ataaattccg caatcaccat ctcccaagac acgagtagaa tcgctaacca
     3961 cagccacaag gttaccccga ttgctaagtt cataagattt taaggggtta tcgcgaatag
     4021 tagtagaaac agaagatacg ccaggagtgt accaaacatt aaaccaatta aaaccccaaa
     4081 ttcccgcttt tggcagggtt tgcattttcc cttgatagaa agagtgcatt tttagggaaa
     4141 gctcttttag aaaataagtt tgagcagcgg actgattttc aggcgcaaaa agctctgaaa
     4201 agacctctgc caagttggaa agattggcat ttaactcatt cattttttat cctttatagc
     4261 attgttgcca ggttacaaat attatctatc tgctttttta tgccctacaa tgaattcctc
     4321 ttccactcat tatcaacacc tttacctctc aaccttgagg acgggttgat gatgggttga
     4381 ggaggagaga aggaggagtg gtatattgga atcaaataag gaaggcatag caacttatta
     4441 attatcatcc agataactta tcaatataaa acagctgcaa aagcactacc attaagtgat
     4501 ttatattcta tacacataga aataaatatc ggattagggc tttttgccta cgctggatat
     4561 atagattaca aattcttcaa ttccatccaa acctaaagct tcattcattg cttcatccaa
     4621 aaaagcgcca atcatacagg ccccggcatc tatggcttca gcggcaagat ggatattttg
     4681 gccaatgtgg cctgcatcaa gatagagata acgatagccg cgttgtccat atctccatac
     4741 agttctatag ggaacagctg ttaaaataaa gttgaccgct gcattggaaa tcatttcttg
     4801 aaacatacat ccttcgtgaa ttttagcggc aatctgttcg gaattatcat acaaaattag
     4861 gcaatgcttg atgggatgat aatagtataa accgggtttg atgccttcca ctcggcgaat
     4921 gtccagaaag gtctctatcg gatgacgcga acctgcagaa ggaacattgc gaaaagtgag
     4981 ttccatcctt tcggtagagc gaaaatcgcg agcccaggaa gaagcccaaa gcaaaaaaga
     5041 tagttcttcc tgttttagtg cttcctggct atattctctt ctgcttctgc gttgctcaat
     5101 tgcttttgct aaaggcatat cgggcattgt tagagtgcca attgcaggta aagatacaat
     5161 ttctccaact cttgctttaa cggcatcagg acgttgaacg cctaaggttt gatcgctttc
     5221 gcgttcatag atataacgag ttaagcggac aaaatcttgc ccaatatttt cggcaacagg
     5281 tcttaaagtc tcaatttctt ttagtacatc ctgcagattt ttttcaaaga actcatattc
     5341 ttcttcggtt atttcagcat ttttcaaaat atcatcatgc gcatagccgc gagttttata
     5401 atatagatag ccaaaggttt tcatatccat agttttctct ccttgtttat agtaggtctt
     5461 ctctgtttac ctgttgcaaa tgttccacaa tttttttcac ttcctctgct ttttccttgt
     5521 tacaaagcag aatggcatcc gctgtttcaa tcaggcagag gtcgttaact cctataatag
     5581 cggtaaattt attggtatct atatagttat tggtggcaga aattgctttt ccttcagcgg
     5641 aaaaatagtt gccatcctca tctttattaa ccaaatcgga aagtgctttc cagctaccaa
     5701 catcgctcca atcgtaagaa acagggacaa ctcctctaaa atctgccttt tccataatgc
     5761 caatatcaat agggattcgg ggcattttag catacaaaga agaaatatct gtttgtaaac
     5821 cattttcctg ccatttgtta gctattgcct tagtaatgtt atcaacctcg ggaaggaagt
     5881 tagtaaaagc ggttgatata ctttttaaag accagcaaaa cattccactg ttccaataat
     5941 aacttccttt tgccagaaat tgttccgcgg ttgctttatc tggtttttct ttaaaattaa
     6001 gcacttttct tacttcaggg gcaatttctt ctccggcttc aatatagcca taacctgtgg
     6061 cgggataagt tggtaaaatt ccaaaagtaa ccagataacc tttttttgcc tgttcttctg
     6121 caggtaaaag cgattgtaaa aaattttcct tatctgtaat gatatgatct gcaggtaaaa
     6181 caaacatcag ctcttgctcg ggataaaaat ttttaagata cataacactt aatgcaatac
     6241 agggagctgt attcatccca aaaggctcaa tgataatatt ttgctcgggt aattctggta
     6301 actgctcctt aattagttca acctgatttt gagcggttac gatataaata tctgtaaaag
     6361 agaattgggg taaaagacgc tcaacggtca tctgcaaaag tgacagctct ccgctaactt
     6421 ttagaaattg tttagggaag ttctttcggc ttttaggcca aaaacgagtg ccgactcctc
     6481 ctgccataat tagggcaatc attgttcaac aacctgcaca tctttaggat aagtgaattg
     6541 ccagatggag gcgggaattt tggtgttggt ttttatattg gaaaaattat atgttacttt
     6601 gtcgttattt ttgttcgtcc agcttaggga ttgaataatg ccagttttgg tatctactac
     6661 tgctgatata gaaacaatga tgggatcact atagggctta agagttacat ttgccaaatc
     6721 acctttgctt gattgaacat taactgagga ttttttccaa tataactgca aaatttccac
     6781 cggattcatt ttcccgaatt ctggacgcat cttgctgcga aaaacagttt tactttcgga
     6841 atcataaagt tctacaattc ccccggaaat cattaaccgt tgaaaactgg gtttcgtgaa
     6901 ccgcataacc attcgccctt tagtaaaata gatatttccc tgataagtaa tgcttttgtt
     6961 aagctgagca aaatagttct cctgttttaa atttgcctga aagctactga agctgctata
     7021 ggtattggca attttctgat gcaatgcttc actggttata cccaaaagag aaatgcagct
     7081 taaaaaaagg ataagcggcg taattttagc gattttcatc tatcactcct atgcgaatta
     7141 aatcttcacg cgtagctaat acatcgcgtg atttactccc taaatgaggg ccaacaattc
     7201 tggctctttc caaaagatca atcaaacgtc ctgctctggc atagccaatt ttaaaatgcc
     7261 tttgtaacat agaaacagaa gcagttcctg aagaaacaac tactcttgct gcttcgggaa
     7321 ataattcatc gtcataatca aaaaagccaa ggctttcttt atcctctaca acaatagaaa
     7381 aatcctgtgt aggtttgggc tgagtagcta aaaagctgca gactcttgca atttctgcat
     7441 cagatacaaa agcaccatga attctttctg gtaatgcttt gcctggaggt aaaaacaaca
     7501 tatccccgtt tcctaaaagt cgctctgcgc ctatcatatc taaaatcacg cgggaatcta
     7561 ctctggacga aacttggaag gcaattcgtg cgggaaaatt agctttgatt atacccgtga
     7621 taactttgat ggaaggacgc tgcgtggcta aaataagatg catccctacc gctctggaca
     7681 tttgagctaa tcgcgtgata ggtaattcta tatcctttcc actggtcatt attaaatcgg
     7741 caaattcatc tacaatgata actatgtagg gaagaggttc caaatcttca tcttcttcgc
     7801 atttttcgtt ataaccaata atatcacgaa ctcgggcatc ttgcaaaatt tcataacgac
     7861 gctccatttc gcgaactgcc caaataaaag tttctaaagc agtatcggca tcggttacta
     7921 cttgtccaat taaatgagga agttcattat aagcagccag ttcaactcgt tttgggtcaa
     7981 ttaaaataag gcgcaggtca tcaggtttgg tacgcataat taaactcata ataatggtgt
     8041 ttatacatac acttttacca cttcccgtag ctcctgcgat gagtaaatga ggcattttgg
     8101 ctaggtctgc aacaattggt tttccggcaa tatcttttcc caaaccgaaa gctaatttgg
     8161 aacttgtctg tttcatctgt tccgaaagca ggagatcttt taaatagatc atatctcttg
     8221 ccagattggg tatttcaatt ccaattaatc cgcgtcctgg aataggtgct tgaactctaa
     8281 tcgatttagc cttgatagcg agtgccaaat catctgccag agaagtaaaa cggctaactt
     8341 taatgccctt tgcgggttcc aattcatatt gagtaataat agggccgata ttaacatttt
     8401 tcacttctgc ttcgatacca aattcagcca gcttactctt taaaatctgg ctggtagtaa
     8461 tgatctggtt ttcaatttct ttatgttccc tatcggatag ctttattggg ctttccaaaa
     8521 attcatcaat tgcgggaagc tgatattctt tatctgttcc ctcagaaaac actcgcttgg
     8581 ttttattttc tttttgaggt ttaggaaatt ccttaggtgt tgaatctatg gaagggttta
     8641 atatagtatg attttggata ttgggaattg tttcttcagc gaccgtttgt tcaggattaa
     8701 tattattggc ggtaattgtt ggcttgggag cttttatttt ggtttctttt ttttgttgca
     8761 gattttctgc ggctgcagat atccaggctt tcaaccgttc atattcaatt aacataatca
     8821 ccgttaaaat cattgcacca attaaaataa tccgggctcc cactccagaa aaaacagctg
     8881 ccagtatccg ataaacaaac aaaggaataa tactgtgaat atattcttgc tgcccagaaa
     8941 caagcagcac ctgcagtaag aacattacta aaattagcaa ataggctttc tgcctgcctt
     9001 tttcagcttc cggagctaga aaatattgaa atgaaatgag agcaataagg ataaatccga
     9061 ttaatgaaaa ccagaaacct aaaagataga taaacacata cccgaaaact attccgaaaa
     9121 taccaacagg attattagta gtggaattgc ctgcttgaaa ccaggaaaaa atactgccag
     9181 cggcttttaa actttctaaa tcgtttttaa tagaattata gcccatatta agtgcaataa
     9241 taactaatat agatagcaaa caaataatac cgctaatagt tcttttctta gcagtgctca
     9301 ttttttggga tgccttatgg ctctttgttt tgtgtttcgg attatttgcc aatgcgttat
     9361 actccttatt ctactatacg ctaaatctga tagagagaat atctccatcc ttaacaatat
     9421 aatcctttcc ctccaatcgg aaaagacctt tttctttaag tgccttttcg ctgcccagat
     9481 ttattatgtc ttcatactga aaacattctg cccgaataaa accccgggct aaatcactat
     9541 gaatttttcc ggctgcagtt acagcattgg aacctttttc aattgtccag gcacgaactt
     9601 catcaggacc aacagtaaaa aaactgatat atcccatcag ctcatatgtc caatgtgtta
     9661 aacggtctct aatggaatca ctgattccca tatcttccat aaacatttgt gcttcttcat
     9721 tatccagttt gcttaattct tcttcaaact ttccggcaat cgaatgtgca gggaaaccct
     9781 tttctctaaa ggtgtttaat gtatcatccc aagcagttaa gttatcttca ttacagttaa
     9841 ttagaatcat cataggcttg aaacaaagaa attgaaaacc gcgaattgct ttttcctctt
     9901 cggaagaaag ttcaagatta gtaagtggca aattattctg cagacaatcc acaatttttc
     9961 ttaaggcttt ttcttcaatt tgaatggctg gagtcttaac tcctctttta taaccaagct
    10021 cgattttttc cagacgcttt tctgccataa tcaaatcggc aagtaacatc tcatcctgaa
    10081 agtgttgaaa ttgcgtttcc ggagagccaa gtggataaag accttccaat tcttcatcaa
    10141 taaagccacg caaaactaat ataaacgctt cggctgtttt taggtcggaa agtaatgctg
    10201 tatcagaggt ttcactctcc ttgtaaaaaa tcccgggata atcgtgatat tcaattgtgg
    10261 cgtagatagt ttttttaggt tgatagagct gggagagctt atcaattctt tcatcagcaa
    10321 cttgcacaac tccaatattt gcttctaaag aggcaggtgc atatttatcg gtgttatgtt
    10381 cgcttcttgt taacgcattg aaaatagttg ttttaccggt tttaggtaaa ccaattattg
    10441 ctgctttcat ctatttttcc ccttctttat atataaaagt gattgaattt ctgcagaatc
    10501 taaaaatcgc caactaccaa caggaagttt tttaagtttt atgggaccaa actgtaagcg
    10561 ctttaaataa gtaacttggg catcaactgc ttcaatcatt tggcgaatct gtctttttct
    10621 gccttcactg attactattt ttaagctcat cccttcgata ttttgcgctt taacaaaaac
    10681 ccccgctggc aatgtttttc cgccttcaat ttcaacgcca tttcttaaga tgttcagttc
    10741 tctattagtt agcgagcggt taatatccac acgataaacc ttttccactt tgtttttggg
    10801 gtgggtaagc atattaatta aaatagtatc attagtaaaa agcaaaagcc cttcactatc
    10861 cttatccagg cgtcccgcat aagaaagttc tttagcttgt tgaggtaaaa gagaatagac
    10921 agtctttctg cccaattcgt cacttctggt aacaacatag ccacgcggtt tattcagaat
    10981 caaataaacc tttttaagtt caggttgaac aattttacca tccaaggtaa cagtatcttt
    11041 tccggaaaaa atctgtgtgc taaggtcttt aactataact ccatttacag aaattctacc
    11101 ggaagtgatg aattcttcaa cttttctgcg actgcctaat ccgcaatctg ctaaatagcg
    11161 attcaggcga atggttttac cagccctcga tactgttttg gaggacggtt ctgtagagtt
    11221 tggcgatgag ctcttcaacg gcttcctctt tggttttaaa tttggcagta ccaccttcag
    11281 caacagcata aagttctgtt accgttaagg cgttattttc ataaagaacc gtgttattaa
    11341 ttaaatcagt aaaagtaact gaaacaacca ttgttaactg ataatcttga atctggttgg
    11401 ctgtatcgta actataaacg ctttccgaaa aacttaagat agcgccttcc aaagtgcaat
    11461 ctggatcttc agtaactatt tttaaacggc catcattacg aatttcacgg ttcaaataat
    11521 tgagcagttt atcccccaat ccgtattcgc tggttcgatt ctcaaaagcc agaatcctaa
    11581 tttttcgcaa atgcggataa gcattcgaat aaaccgaata aaaacaactg cttaagatta
    11641 acaaaagcag aacaggataa ataaatttct tgaccattat tccatctcca gaaaatgctt
    11701 atattattgt caagataaac cttgcttgaa cagatataaa atatatataa aacctaaaaa
    11761 agggaggtat ttatggccaa gaaatttata tctaaagctg aagctgccaa gaagttaaag
    11821 gtctcggaac gagtcattca agaaatgatc gcaacgaatg ttttcgaaag taaactcgta
    11881 ggaaaaaaca ttaaaattga tgaggattcc ttaaacgagt ggttggaaaa tctaaatgaa
    11941 atagacgaaa aaatgcttgc cctaaaaagg gttatttgcc atttcgaaga gtatatgcgt
    12001 ccagaaaata ttttcctgga ttttacagcc gaaaataaat acgatgctat ccgaatcctt
    12061 agcgaaaaag caaaagacct aaaactggtt agagatgctc gctggcttta cgaagttgta
    12121 gttgcccgcg aagaattggt ctccacagct attggtaatg gagttgctct tctgcatcct
    12181 cgtcacttac atccttccaa aattaaaacc cccagcattt tgtttgggcg ctcttcggaa
    12241 cccgtagatt ttgacgctcc ggataataaa cctgtgaata tcttctttat gctcttattg
    12301 cataacgata aacaacatct tttttcgctt tcctatattt ctcgtttagt tatggatcaa
    12361 gatgttttaa aaaggttaac agaagccgct gatgctacag aaatacatca gttgctaaca
    12421 gttattccag agcagacaaa taaatgaaac ccataatccg tattatctta ggcagtaaaa
    12481 gtgatttgga taaaatcgct gaggccaaag atgttctgga acaatttaaa gttccttatg
    12541 acttatttat ctcttctgca cacagaaatc cggataaaac tattgccctg gcccaaaatg
    12601 ccaaaagcga aggaattgaa gttataattg ctggagctgg tttggctgct catttacctg
    12661 gaattattgc agcaaataca actctaccag ttatcggaat cccttttgcc gcaggagcat
    12721 taaatggatt ggattctctg ttttccattg ttcaaatgcc tccaggagtg ccagtggcca
    12781 gtatggcaat aaatggagcc aaaaatgcag ctctgtttgc tattcaaatt ttggctctga
    12841 aagacaaaga actggaagca aaatttgcta cctacaaaaa agatctaagt gcttgaggga
    12901 ctggatgttt aaaccctcag agaaaaaaag gagttacgat gtataagatt gttttacttc
    12961 gccacggtga aagtatttgg aacaaagaaa acttatttac tggctggacg gatgttgacc
    13021 tttccgaaaa aggcatagtt gaagctcaga atgcaggtaa acgcttaaaa gaagaaggct
    13081 ttgttttcga tgaggcttgg acctctgttt taaaaagagc aatacgaacc ttatggatcg
    13141 ttctggatga aatggatcag atgtatatcc ctattcataa tgactggcgg cttaatgaaa
    13201 gacattatgg agccttacaa ggtttaaaca aagccgaaac agctatcaaa tatggagaag
    13261 aacaagtttt actttggcgt cggagttatg atacacctcc acctcctttg gaaaaaaatg
    13321 accctcgtta ccctggtttt gatccccgat atgcaaattt gagggaagat caaattccgc
    13381 tttgtgaatc attgaaagat actgtaaaac ggacaatgcc tttctggaac gaaattatta
    13441 tgcctcgttt agcagcaggc agaaaattga ttgtttctgc gcatggaaat agtttacggg
    13501 caattgtaaa aactttgaat aatattagtg atgcggatat tgtaagcgtg aatatcccaa
    13561 cgggaattcc gcttatctat gaatttgatg ataacttacg ccccaaaaaa aattattacc
    13621 tggcagataa ggcagaattg gaaaaagcac aacaagcagt tgctgatcaa gggaaggtaa
    13681 aaaaataaaa ccagacctta taagctagta attacatatc ctgtaagtca ggagtcattt
    13741 tgtgcttcta tcacatattt cctatgtgat aattgtaata acacattgaa cttatcaaag
    13801 cgatagtaat gactcctgat ttaccgacat aaaaacaaaa ttaggtctcc agaaatcttg
    13861 ccagagcaaa aagaaaatct gacagccgat taacatagct aagtagttca ggggcaatat
    13921 gttccttatg cgaaagggta ataattctgc gttctgctct tctggcaaca gttctacaaa
    13981 tatcaagctt agcagaaaca ggtgttccac ctgggataac aaaaccatca aaatgcaggt
    14041 tcttttccag taaataaaca tactctgtga tattatcaac atcattttgg gaaattggat
    14101 caggaaaaga tccatttttt gtggccagtt gtcccataca ccgggaaagg ttttgttgaa
    14161 tttccctaag agcatcctta tatacagaat tgggaagatg atgtctacat tcacttatcc
    14221 aggcatttag ttcatctaaa gttccatagg cttcaactcg taggtcatct ttaaaaaccc
    14281 tttcacctga gtatagagat gttttcccgc tatctccacc tttagtaaca atgctcattt
    14341 agttatctca ttgcgataac ggagatttca attttgccgc ctttaggtaa acgagaaacc
    14401 tgaacaactt cgcgagcagg atatggtgca ctaaaataac gggtataaat atcgtttaga
    14461 agagtaaaat catccaaatc tttcagaaaa acggtaactt tcacgatatt cgacatcccc
    14521 attccagctg catccagaat agatttaata tgattgaaca ccaaatttgc ctgttcttca
    14581 aaaccttccg gaatactaga agtgccagga tcaataccta attggccact aacgaacaga
    14641 gtattattca taagtatggc ttgcgaatag ggacctatcg cagccggggc acgatgcgtc
    14701 ataacagatt gcattttatg ctccttacat ttttttgcta ttatccctat gcagtaatat
    14761 ctgtcaagtt tcataataac aaaaccgaaa acaaaagttc attgaattag cgataagtgc
    14821 cggtaacggt aagaatattt atatatcccg ataatcctaa aacttctaaa gtcctcttct
    14881 ctaaaacaaa atctgcacat ataggtgaga gcgatagtaa aaataaattg ccaattctgt
    14941 aatcttgttt atatttaata gtaaaggata tgtgaggagt aaaagatggt tcaagaaccc
    15001 caagatttcc atgagtttat gcccaacgaa gtttggaaat ggaaaatact ggaagaaaaa
    15061 attgaaaagg ttttatctct gcacgatttt caagaaatac ggctttctat tttgcaagat
    15121 tcgcagatat tagaaaatgg gataatggct ttagttcaag gtaaagaagc tgattcagca
    15181 atgaagggcg ttttgtcgct tgctaatcct gaagataaat taagtttgct tagtttgcgt
    15241 cctgagggca caatcagtgt tttgcataat actgcgcgga ttgccaatcc gggtgatatt
    15301 catcgttttt actattcagg ccctatgttt cgtaaagtgg acaataatca gcagatggaa
    15361 ttttaccagc tgggcgtaga actattaggt agtgatagta ttctatccga aaatgaagtt
    15421 atcgatttgg gaattaaact gtgtaaaaca cttggtttga acgatgttcg cttagaatta
    15481 aattcttatg gctgttccaa ttgtcgaaga ccttttttta atgacctaca taaatatctg
    15541 gaagaacata aaagcgaata ttgcctttcc tgctataatg aactttcagc taatcctttc
    15601 agtcaggtgc attgcaaaga cccaaaatgt aaaaagtccc ttgccgaagg tcctctgatc
    15661 cataattatc tatgcaaatc ctgcaaagct gattttagaa aagtaaagag cattcaggca
    15721 aacttaggga atcagtataa ggtaaatcct cttctaaata aaaactttgc ctattataac
    15781 aaaaccgtct ttgattttat cgctcaaaat gcaggaaagg aaactgttat cgggggcgga
    15841 ggacgctatg attatctgtc ttctctaatt acaggaaaaa acatccccgc tgttggtttc
    15901 tacctgaatt tggataccat ttttgatatt atggaaagcc aaggattgtt tgcaacaaaa
    15961 gatcctgaat ttgtagttta tatctgtgcc gaaagcccaa acctggaaac gatgaccctg
    16021 caaattgcgc aagagctaca ttctcaacaa gttaaaacca tcatctcccc ggagtgcaaa
    16081 caaataaaag aagaaattgc cattgctaaa caaaataaat gccccattat gattatttta
    16141 cgcgaggata acctccgcga aggaaaagtt cttattcgaa atctggtgaa agaagaacag
    16201 aactacatct ctttgaaaga taccctgtcc actatcttgc ttgcaagaaa atctatgcaa
    16261 agctgaaaat cacgcaacac cgaaaagaat atctacttta ttttgtgggg tgataagtta
    16321 ggcgactttg tccactcaga gcaaaagcaa ggtaagcttg cagtattgaa tatatagtaa
    16381 atcttgataa taccctcctt cggtactgtt cgtttggtgt aaaaacttac ccatccagtt
    16441 atctgcgttc taatttttct atcgctttcc aaggtaatca tcctgtatct tgcttagata
    16501 gttgtggtgg agataccgtg gagctggtgg ggaaaaaacc gatagacacc accctatctg
    16561 ctccataact aatcaagcaa ggataaggtt attttatagc aatagaatca attagagagt
    16621 atatgtggca aaggggctgc cggatgttga tttgtaaaat gcggaacggc ataatattgc
    16681 agttgatttt tcatctgtga tctatcggag attaataacc cgctttttgc taactacgca
    16741 atttgagaaa aaataagggc tggtatctaa ctttgttcag tttgcttgcc agcccttaga
    16801 gctaagtgtg ttatttattc gtttattgag ttatgattaa accttcttgg ggattccaat
    16861 ccagcataat actctttccg gtggtaaatt gtccggccaa gatatgtctg gctaaagtat
    16921 cctcaatttc cttttgaata aggcgtttta gcggtctggc tccaaatgct ggatcataac
    16981 cagcttctgc aagatggtca attagatttt cagaaaaatc aagctggaga ttgcgattgg
    17041 caactcgctc tgccagcagg ttcaattgca gttttacaat ctttttaata tgatccttat
    17101 ccaaacgatg gaagaggata atgtcatcta cccggtttaa gaactctggg cggaaatatt
    17161 gctgtaagat attcattaac tgtggtttta gccgttccag ttctttatcc gacgcttcat
    17221 aaatttcctg cgaaccaata ttgctggtca taataatcac acagtgctta aaaacaactg
    17281 ttctgccttt accgtcggta agccgtccat catctaaaat ttggagcaaa acattgaaaa
    17341 catccggatg ggctttttca atttcgtcaa aaagcaacac agaataaggt ctgcgtcgga
    17401 ctgcttccgt taaataaccg ccctcttcat agcccacata tccaggaggt gctccaatga
    17461 gtcgagcaac actatgtttt tccataaatt cactcatatc taagcgaatt aaggcttttt
    17521 ctgtatcaaa tagataactt gccaaggttt ttgcaagttc cgttttacca acaccggtag
    17581 gacctaaaaa gatgaaagaa ccgatgggtc tgttttcatc gcttaagcca ctgcggctac
    17641 ggcggatagc attggccaga gctttaattc cttcttcctg tcctacaact cgttgggcaa
    17701 gaacattttc cagttcaagt agtttttgca tctcgctttc tgctaatttg gaaacaggga
    17761 tgcctgtcca tttggaaaca atttccgcta ccaattcttc atctacgatt tctttcagta
    17821 atcggtcttt tttggtgagt gtttcgttct gtaacagttt ttgtagttct ttttctttag
    17881 cggcgatagt accgtaacga agttgagcgg ttttttcata atttccctcg cgttcagctt
    17941 tttctgcttc acctttcaga gtatcaattt ctgttttcaa ggcacgaatt tgttccaaaa
    18001 cctgtttttc tttttcccaa cgaaggcgta aagctctatt ttcttcgttt aattctgcaa
    18061 tttcttcttt tattttttcc agccgttcct gtgataaggg gtcattttcc tttgccaaag
    18121 aaaggcgttc aatttccagt tggcgtaacc gtctttcaga ttcatcaagt tctacaggca
    18181 aggaatcaat ctccattctt aataaagcac aagcttcatc aattaagtct attgctttat
    18241 caggtaaaaa acgatctgca atataacgct ctgaaagctg agctgcagcc actaaggcat
    18301 tatcactaat ttgaactcca tgatgaactt catatttatc ccggatgcct cttaaaatgg
    18361 aaatcgtgtc ttccactgta ggttctgtaa ctaaaacggg ctgaaaacgc ctttccaaag
    18421 ctgcatcctt ctcaatgtac tttctatatt ctgttaaggt cgtggctcca atgcaatgca
    18481 aatttcctct tgctaatgct ggttttaaca tattggaagc atctacggaa ccttctgcag
    18541 caccggctcc aacaactgtg tgcaactcat caataaaaag aataatgcgt ccttcggatt
    18601 tttccacctc cgaaagaacc gctttcaaac gctcttcaaa ttcgcctcgg aattttgctc
    18661 cggcaattat tgctgccata tcaagttcca acagatcttt gtccattaaa ttttcgggaa
    18721 catcgcgggc tacaattctt cgggctaaac cttctacaat agcggttttt ccaactcccg
    18781 gctcgccaat tagaactgga ttattcttcc ttctgcgagt taaaatctga atgcaacgcc
    18841 gtatttcttc atcgcgaccg ataaccggat ccagcttttc cattctcgct aaacgagtta
    18901 aattacgcgt gtatttttcc aaagcctgat attttgcttc tggactttga tcggttacgc
    18961 gctgattacc acgcagttct ttaagcgcta aaagtaattt atcagcttgt aaaccttcac
    19021 ttttaagaat attcgctgct tctttccctt taactaaaag agccataaga agatgctcgg
    19081 tagaaatgta gtcgtcctga aactggtctg cttccttctc tgcctgatgt aaaatgtcca
    19141 aaacttcctg agatagatac tgttgagaac taccactaat cttgggaagc ttgtttaagg
    19201 cattttctac ttcacttttc actttgggaa cagagacctc cagcttctgt aaagcaggta
    19261 aaatcaatcc ttcttcctgg ctcaacatag cctgtaataa atgcaggtct ttaatttcct
    19321 gatgaccata actggaagct aaatcctgca tatcagcaag caactcttgt gactttattg
    19381 taaatctttg tgtattcata tatacctcca gtataagta
//
LOCUS       DAOSTH010000024        18773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3103_length_18773_cov_13.528636, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000024 DAOSTH010000000
VERSION     DAOSTH010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 18773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18773
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3103_length_18773_cov_13.528636"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            316..1488
                     /locus_tag="PLB65_04590"
     CDS             316..1488
                     /locus_tag="PLB65_04590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ77840.1"
                     /translation="MRKKWWIIIGIIVLIVAGLLFAKSCGKKKQPEKVQSTEEETYTV
                     KRGDISSRIEITGEVQPKAMVSLKSKVSGKIVKFYADENDYVTSGQIIADIEPDYNQA
                     NTLFNTKAMLQKAEINLKNARKDLADKTVLLKKQFISQEEYDKASDALQEAEIEYAQA
                     SSQYEMIRDLDVPGKVTHVYATSSGVVIERNINEGEMVQSSLTSYGEGTVIMKIADLN
                     QMIIKSNINEVDISKFKLSQNAEISLDALPYEKYEGRVVRIAPQAIIENNAKVFPIEI
                     SINTNGKIAKPGMTANVTILADSRENVLVIPIRAVFSNDKNQDIVYLVTGNTTSTPAT
                     KANDKEKTSATPNYVSTPIKLGTNDLLQVEVIEGLKEGDIILLNEPGTAKNNFMMM"
     gene            1491..2507
                     /locus_tag="PLB65_04595"
     CDS             1491..2507
                     /locus_tag="PLB65_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ77841.1"
                     /translation="MIKIENLSRNFGNIKAVDDISFCIQDREILGFLGPNGAGKTTTL
                     RMIVGYLQPTSGNIEIDGESIFANPIKASSQIGYLPEHNPLYDEMTVIELLAYFASLR
                     KLKRDYFKERLDYVVKNCGLKEVLYQKIGTLSKGYRQRTGLAQAILHNPRILIMDEPT
                     SGLDPNQIIEIRELIRELGKEKMVLLSSHIMQEVQALCDRIVIINKGKIIVDDTKDNL
                     PNYLKKSRLLTLEVQGDNVDFSDFLAMYPFLEMEVFLQTETICKMGFYETSEIDLRKE
                     LASFIRQKGWLILELSTSKLSLESIFHELTEGEYSSAKTEVASPEQEEQNAHITSIDE
                     EEQQ"
     gene            2504..3226
                     /locus_tag="PLB65_04600"
     CDS             2504..3226
                     /locus_tag="PLB65_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOQ77842.1"
                     /translation="MTTVFTIAKKEYQVAMRLVSTYIVFILFLVVIGVFFSNMVFKIG
                     QAELRFLFEMIHIIFLFYIPAITMGSIAKERQSGTLELLSTLPIKLSSIIWGKILAAL
                     YQIITIILLSLTFFVIIVIFGEGIDYGAIICGYIGLIMVGFAYASIGVFASSFPSNQI
                     LAFVLALLISAAFYVLKFVLPLLPFRVLPVVQYLSFDYHLSSFLKGVIDTRDILFFLG
                     VTVIFALLAQFNLQSRNLMQER"
     gene            3227..4801
                     /locus_tag="PLB65_04605"
     CDS             3227..4801
                     /locus_tag="PLB65_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HOQ77843.1"
                     /translation="MKKAKKISSILSNLLIKLGIVLMILLIGSYAKIRWDFSHNKAYS
                     LSKISKYAVSKLEDNMVVKLYASKELPPQMNTIDRYVKDLLEEYRQAGKGKFHYDIIT
                     GLTQEELKNKTQLYGIPTMYFQIFENDKTTTKEIIYGLVFEYQGNYESLYVLPEMQNK
                     LEYEMTLKIQKISRYTLPDVTAFTDSLYTIMPKQKYENELNSNYNVHFTNLKEPPQQT
                     EVMIFHSGFDSLTTVQLYNLDQYIMKGGKLVVLADKMASDGERVLELDSNLFDILENY
                     GIKFSNAIAMDIFCDSRQMGVDTNISFPVYPVLRGSQHPITQNISNIVIYMGNGIIFN
                     KKPGLKFQTILATSTSSAFLEGPDYQLDASMFLHPDPEVFVNPPIPLGAIVEGRMESY
                     FAHKPDAKQPGFISEVKDGKIVVFADRELYIDPDNPIYENRYHIILNAVDWLMDRPDM
                     IAIRSRHLQKSILDIPYYMHKHNLVWGEPVKTERRIKLIIKIVAIVLPSLLLVGIGWI
                     SSIMQKRNLLLKNEEE"
     gene            4788..5711
                     /locus_tag="PLB65_04610"
     CDS             4788..5711
                     /locus_tag="PLB65_04610"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HOQ77844.1"
                     /translation="MKKNKLILLLILLLLIGLYFLLRTKQPQEKLLPVFDLDTLAINR
                     IEIYDAQDTLKMVKQGNCWRLIYPVNWEADSLKIHSLFKDILTAKYSKTPMGTGKEAI
                     LKFHQRDEETLHLIVSDGKKTVHTLFSNLNNPYDYFRYAGSEEIYQVKTKVTNVYNTD
                     LANWRSPHIVSHQENDLVKIEVTHPKNKYTLTRKGLDWYYQDAHQNFQIHPYNRAMIK
                     ILNILEHLDTYIFVDNAQAEYAAKFDDPYCTVKLFLTNNRTQDLKFIEHYQGHYLMMV
                     DNDPTVLFVVVWDTVFRFTRPPDVFRMQELG"
     gene            5714..6148
                     /locus_tag="PLB65_04615"
     CDS             5714..6148
                     /locus_tag="PLB65_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ77845.1"
                     /translation="MLTIKRIKQLNNNLYQKVSELWVETGISNPERKDSLEIVQQNLE
                     NTGTMIIAEEDENLIGVVWVTHDFRRLYIHHMAVKPNKQNQGIGTKLMEECVTIAKEF
                     GYQPKLEVSIDNPAARHLYEKFGFKELTGYSVLIKRNILTDL"
     gene            6189..7286
                     /locus_tag="PLB65_04620"
     CDS             6189..7286
                     /locus_tag="PLB65_04620"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOQ77846.1"
                     /translation="MKKRLLVATGGGDCPGLNAVIRAVVKRASHEHNWEVLGSVDAFN
                     GILQDPMHIIELTPERVAGIHVQGGTIIGTTNKGGPFTWPVKNQDGTWSTVDRSQDFM
                     DRLRYQNIEALINIGGDGSQRISHKLFEIGCPIIGVPKTIDNDLSSTDFTFGFQTAVD
                     VATDAVDKLVTTAASHHRILILEVMGRYAGWIALHSAIAGGAEVCLIPEIPYSIDKVM
                     DAINQRIDKGHDFANIVIAEGARPIDGEMTYIESEEPGAMKMKLGGAGLRLSQELKDA
                     GCTIDIRETILGHLQRGGTPNAFDRILASQFGVKAFELVLEQKWGSMVAYHHPNIVAV
                     PLAEAIRKYNYIDVSSDLVATARGLGISLGD"
     gene            complement(7329..8618)
                     /locus_tag="PLB65_04625"
     CDS             complement(7329..8618)
                     /locus_tag="PLB65_04625"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ77847.1"
                     /translation="MKILYISYFYPPLAGPASLRNLKTVKYLSQLGNTIDVLTVGNIV
                     YNFYDNGLLAKCEQNRIIHIPSFDPMSILEKLSGKNKTMSNNIYHQTPERIKLFIRHL
                     YPLDDKVLWLPNLLKTAKKTLAGDSYNLIYVSCGPFSSAVAARYLSKHFKLPYVLDMR
                     DYWTLLNDYNLQGTYLNRLHSQHHEKRILKDAALIVTATKGIAEDISNHFNKELQSKT
                     FVLYNGHDEEDFSTLPKMETNHDQYTLSYFGTITAKRSLKWLLRALKNLKQENKLPVG
                     FSFNLYGNYHRETYQEIQQSGIEEIVKVTPQITHKEALAVMQASDALLLLINSDSPKG
                     TLTSKVFEYLRIGKTILALIPKQGEAAELLKESGQNFICPMESSVAIKKELEKLFAQR
                     DDEHHFHYLAEKYERKKQVEELNQKLQKINKTQFNNT"
     gene            complement(8615..9748)
                     /locus_tag="PLB65_04630"
     CDS             complement(8615..9748)
                     /locus_tag="PLB65_04630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ77848.1"
                     /translation="MKICIVTNTHPTNDVRLYYKLAMSLAKENEVYLITTNGVINNKL
                     NPYQMVVDASSRWAAIWQLGKKVRDLKPDILICVEPLTLLIAWVLKHKLQLKVIFDVH
                     EFFAIAFSERFPRFLRFPAYLFYQVSLKQLLKIADAVFTVNREMEKQLLSGNKRITSI
                     VLPNYPVKNVWDYECNKPGSLEQICEMNFDFIYIGGLTEDRGVFKILKMVSILKQDFP
                     FLKVLIVGKFFKKEVEQRFNQSINDYNLNALIYYQSWIPAEKIGLLLKRSKFGLWIFN
                     PKNKRLQLSTPLKVLEYLAAGLPVLTIKTPIMKALIEKNGLGICSAYQSKALADACAK
                     LLNLKQSEYQAMSDKCIKLTENKFNWEALEPELFKIINGLNSK"
     gene            complement(9965..11266)
                     /locus_tag="PLB65_04635"
     CDS             complement(9965..11266)
                     /locus_tag="PLB65_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfJ domain-containing protein"
                     /protein_id="HOQ77849.1"
                     /translation="MFSKDIQSTGRPYKEIEKRAIIFKEEEGKLYHFTKRYIKVIRLG
                     NPYPAAYQKKLKDNYWECFLPTQPFPFLFYPPKPENLKSNNYAIRLNALHKESIYKSM
                     EAYIGAEKIELLNGFTSRFWFILCLLFRGGNYALELMQTNPAIGYLLSAYAVFHPLKS
                     KKYWQSSTKLITKKRKDILGYFGFPACETMVKTFAKIKPESCSTNIFFILRTSLQNNP
                     NLLRTLSYFPVHNDYSLFLVCKRLDIMQNYNLIAEIANMNQEEVLNINSQINDIYRML
                     EVLRENGLQDRKVILKNITDLNTYHDELVIQILKLKKIDFVPLPEGIAKDIKCHTLWI
                     EHIHNSIELYREGMEMHHCIYSYLSDIRAKTRFVARMLYPERLTILYENTKNDGLKLK
                     DARGKYNTTPDQNSFCLIKLWLAGVEMDIIDSYEPISQTSH"
     gene            11450..11620
                     /locus_tag="PLB65_04640"
     CDS             11450..11620
                     /locus_tag="PLB65_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOQ77850.1"
                     /translation="MAVKIDKETCLGCGACVDGCPVSALKMVDGKAECDASLCIDCGA
                     CIATCPVQAISE"
     gene            11878..14442
                     /locus_tag="PLB65_04645"
     CDS             11878..14442
                     /locus_tag="PLB65_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77851.1"
                     /translation="MKRLSIFLGIITFISLCYSIQVSGNQSGIWSPENNPYEVIGAIT
                     VPSTDSLIIQAGVEVHITGSYQITVQGILNAYGTEADSIRFLNMQTYPTALWPGLRFE
                     NLQLTSHLNYVYIEYATYGVRTINSPIAISHCHLNLCEKGIELYGIGSAIPLPNVVEY
                     SLIENCTQNAILVTSNSNAYIRYNEIRYNGTGPQFRAAIQMGNQSGVNQCNPNILNNH
                     IHHNYKQGISAWDIASSGSINPQILNNLIEYNYTGIYLLQASGYVADNQINFNFIPGD
                     MNSGAGVMVSGVTSIPYFERNHIEGNYTGFYITNNGKPVLGDLAIDHIWAQGENTIIN
                     NIDASGNNNSVFCDAYANASFIIMAENNFWGFSSAAEIAETINDHNDNAALPTVDFEP
                     FIIPVENTAITGNYTYNGTAQIAASRLELITVSESNIQVTVPLSELTFSLETDIAEDF
                     YVQVVLTETATGKDFYGCAGGYSNPTVFSPNPGNSINVGTIEVTDSPLPRYEYVAEPF
                     QEDNLTLYPLATGRGLYSYDKLDIVYAEGDYLYLKRNFWQTPTGEVVTELPVGTIYKK
                     YLNINAGDSWQQTEVIYPGNVTFTSTVYVDNCGGEFSNSDKLFTRKDAQGNIVDKWLK
                     LDSGELLFHYQNHYTIAKERVQYIPGNNDPLTAGNITLFIAEPLNDNPTYLVYDPNIQ
                     NQVRLFWQAPSTGNYSWTNYRIYENDQQIAEIPFSLSEYVVSPFNPQVTTSYYITATD
                     GTNESEPSNSVTVIIIGTEDPIQKPVVINVFPNPVNFVTGSALKIEMKNLQSRNAELE
                     IYNIKGQLVFRQNNFEANTILWNGIDNKGMRSASGIYFLKVKVTGDKPVTRKILVL"
     gene            complement(14907..17705)
                     /locus_tag="PLB65_04650"
     CDS             complement(14907..17705)
                     /locus_tag="PLB65_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HOQ77852.1"
                     /translation="MKKIVLSLILIGCLFSLGAILPPEYSALPLQQDPEVITGILDNG
                     LKYYIKQNAKPEKRIELRLCIDAGSVDEDDDQRGLAHFVEHMAFNGSKNFPRTEMVDY
                     LTSIGMGFHNGLNGGTSYNSTVYQFKLPTDDEAKLLKGISILSDIAWQLSFEPSEIER
                     ERGIIQEEWRLGQSAQQRIQDQIDKVRFAGSRYAERNPIGTIENLKSFKHNSLIRFYQ
                     DWYRPDLETVIIVGDCDPKKMENVIKEYFGAIPKRENPRPKVHYSVPDNIEPRAITVL
                     DKELPYTMLRSTWKVKSEPITELGSFYYSIKQDLFFTLVNARLQELSHQSNPPFSFAY
                     IYNQNWLKGFVATDCIMFANEGQSEEAFRTLITELARVRQHGFQPGELDRAKQIMIRE
                     AEQRVAEKKTMNSEDLVWEILDDANSGNTILSFEQYEQLLKGLINEIALSEVNEMVDE
                     VITSENLTLSLAGTAKEGVKYPSKDDLLNIYHQATAQELEPWEDITVNEPLLETIPNP
                     GKITKEKVYPKSEIKQWVLSNGIQVYSKKTDFKADEVIMIAQSPGGKAKLKPENYQAA
                     DLLNQYFYVAGFGNFDGNALKKAMAGKIADVYPTISNYSEGWRGSCSPQDMELMFQML
                     YQYNYAPRYNEAAFSSTVASMKTWTQNYLLDPSTAFFDTLDALIYENHPIKRQLHPED
                     LDTLTLKQIEDIYKDRFSDYSDFTFYIVGNFEEEQLKNYCRTYLANLPAKGRIEKRTD
                     VGVRTFTGKKDIVFTKGSDRSFVSNVTVGKTSVSPQTKVNQNALMILANDKLRENVRE
                     NMSGVYAVTVWSSYDFLPKLSFVTQTWMGCDQIRAKDLNAATFATLDSLKNGLFADKY
                     LDATRTTLHKTYEENINTNKYWINNMSSNVSLGLPIDCFMDYPALYDNVNKKAIMEAA
                     KQYFGFDKSCLSVYMYPE"
     gene            complement(17718..18335)
                     /locus_tag="PLB65_04655"
     CDS             complement(17718..18335)
                     /locus_tag="PLB65_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77853.1"
                     /translation="MKNRIWIILSFAIILLMLSACESGAKLKVYNRCSYPAYIKVEGQ
                     EFKTIGADEEVEYNIDTKTQYIFAGDVKKKLLIWLKGETYSIHDKDLNAYLDSTWIEV
                     QAGEIYKIFLLPNRASVKIVNASEQTISRAEIWQHSDLTHLKVGVLTNIMPGENEFLC
                     VEYGTDYYYQVEIYLSDGTHQTFGSYNTILDKDEQFLITYRSEYN"
BASE COUNT         5868 a         3411 c         3488 g         6006 t
ORIGIN      
        1 attggtgacc ttatgcaaag atagagaaaa gaatgaaatg atgtggaaga cgataaacaa
       61 caccgatttt aactcaaagg gcgagataat caggctgtcg gctttaagtc aaaatcggca
      121 tatactatga tattattggt gaccttatgc aaagatagag aaaaggatga aatgatgtgg
      181 aagactacaa acaacaccga ttttaactca aagagcgaca acaaagaaag cgaatgactt
      241 tttaagttga cacaaattct gctttttcca cttgtgtttt attataagtg aaatagtcaa
      301 aaaaatagag gtattatgcg taagaaatgg tggatcataa tcggaataat cgttctgatt
      361 gtagcgggct tgctttttgc caagagctgc ggcaaaaaga aacaaccgga aaaagttcag
      421 tcgacagaag aagagaccta cactgtaaag cggggcgata tatcttcccg gatagaaatt
      481 accggtgaag ttcaacccaa agcaatggtt tctctgaagt ccaaggtgag tggtaaaata
      541 gtaaaatttt atgctgatga aaatgattat gttacctccg gacagataat tgccgatatt
      601 gaaccggact ataatcaggc aaatacattg tttaacacga aagcgatgtt gcaaaaagca
      661 gaaataaacc tgaaaaatgc gcgtaaggat ttggctgaca agactgttct tttaaagaaa
      721 caatttatct cacaggaaga atatgataag gcatccgatg ctttacagga agccgaaatt
      781 gaatatgctc aagctagcag tcaatatgaa atgatccgcg atcttgatgt tccaggaaaa
      841 gttactcatg tttatgccac ttccagcgga gttgttattg agcgcaatat taacgaagga
      901 gaaatggttc aatccagttt aacgagctat ggagaaggca cagtaataat gaaaattgcc
      961 gatttgaacc aaatgatcat taaaagtaac atcaacgaag tggatatttc caagttcaag
     1021 cttagccaaa atgcggaaat atcgttagat gctcttccct atgagaaata cgaaggtaga
     1081 gttgttagaa ttgcccctca agctataatc gaaaataatg ccaaggtttt ccctattgaa
     1141 atcagtatta acaccaatgg taaaattgca aaacctggaa tgacagcaaa tgtaactatt
     1201 ctggcagatt ctcgtgaaaa tgttttggta ataccaattc gagcagtttt tagcaacgat
     1261 aaaaatcagg atatcgttta tctggtaaca ggaaacacaa cttctactcc cgcaacaaaa
     1321 gccaatgata aagaaaaaac aagtgctact ccaaattatg tatcaactcc cataaaactt
     1381 ggtactaacg atttattaca ggtggaagtg attgaagggc ttaaagaagg agatataatt
     1441 ctgctgaatg aacctggaac cgcaaagaat aactttatga tgatgtaata atgataaaaa
     1501 ttgagaattt aagccgtaat ttcggcaata ttaaagctgt tgacgatatc agcttttgca
     1561 ttcaagatag agaaattttg ggctttttag ggcctaatgg agcaggtaaa acaactaccc
     1621 ttaggatgat tgtgggctat ttacaaccca cttcaggaaa catagaaata gatggagaaa
     1681 gcatctttgc caatccgatt aaagccagta gccaaattgg ttatttacct gagcataatc
     1741 cgctttatga tgaaatgacg gtaattgagt tattggcata ttttgcttca ttaagaaagc
     1801 taaaacgaga ttattttaaa gagcgcttag attatgtagt taaaaactgt ggattaaaag
     1861 aggtcttata tcaaaagata ggaacgctat ccaaaggtta tcgtcaacgc acaggtttag
     1921 cgcaggcaat tttacataat ccacgcatat taattatgga tgagcctact tccgggcttg
     1981 accccaatca aataattgaa attagagagc ttattcgaga gcttggtaaa gagaaaatgg
     2041 ttttgctttc cagccatata atgcaggagg ttcaggcttt atgtgacagg attgtaatta
     2101 tcaataaagg caaaataatt gtggatgaca cgaaagataa tttaccaaat tacttgaaga
     2161 agagcagatt gctaaccttg gaagttcagg gtgataatgt cgatttttca gattttttgg
     2221 caatgtatcc ttttttggaa atggaagtgt ttttgcaaac tgaaactatc tgcaaaatgg
     2281 gtttttatga aacctcggaa attgatttgc gcaaagaact ggcgagtttt atcagacaga
     2341 agggttggtt aattttggaa ctttctacca gtaaattgag cttggaatct attttccatg
     2401 agcttacgga aggtgaatat agttctgcga agacagaagt cgcttcccct gagcaagaag
     2461 aacaaaatgc tcatataact tctatagatg aagaggagca acaatgacaa ctgttttcac
     2521 tattgccaaa aaagaatatc aagtagctat gcggttggtt tctacctata ttgtgtttat
     2581 ccttttttta gtggttattg gggtcttttt ttctaatatg gttttcaaga ttggacaagc
     2641 tgagctacgg tttctatttg agatgatcca tataattttc ctattttata ttcctgcaat
     2701 aacgatggga agcatagcaa aagagagaca gagcggaact ttggaattat tatccacgct
     2761 tccgataaaa ctctcttcca taatttgggg aaaaattctg gctgcattgt atcagatcat
     2821 aacaattatt ctcctctctt taactttttt tgtaatcatt gtgatttttg gtgaaggaat
     2881 agactacgga gcaataattt gtggatatat aggattaatt atggttggtt ttgcttatgc
     2941 ttccataggt gtttttgctt ccagttttcc cagtaatcag attttggctt ttgttctggc
     3001 gttgctaatt tcagctgctt tttatgtgtt gaaatttgtt ttaccactct tacctttcag
     3061 ggttcttcca gttgtacagt acttgagttt tgattatcat ttaagtagtt ttctaaaggg
     3121 agtgatcgac acgcgcgata ttctgttctt tcttggtgta accgttattt ttgcattact
     3181 ggctcaattt aatttacaga gcaggaacct gatgcaggag cgttaaatga aaaaggcaaa
     3241 aaagataagc tccattttat ccaacctatt aatcaaactg gggattgttt tgatgatttt
     3301 gctgattggt tcttatgcta aaatacgttg ggatttttca cacaataaag cttattctct
     3361 tagcaagatc agcaagtatg cagtgagcaa actggaagac aatatggtag ttaagcttta
     3421 tgccagtaaa gaattaccac cccagatgaa tacaatagac cgctatgtga aggacctttt
     3481 agaagagtat cgacaagctg gaaaaggtaa atttcactat gatattatta ccgggctcac
     3541 ccaagaagaa ctgaaaaata aaactcaatt gtatggcatt cctactatgt atttccagat
     3601 ttttgagaac gataaaacta ctactaaaga gattatctat ggtctggttt ttgaatatca
     3661 gggcaattac gaatcattat atgtgttgcc tgagatgcaa aataaactgg aatacgaaat
     3721 gacgctaaag attcaaaaaa tatcccgtta tactttgcct gatgttacag cttttaccga
     3781 ttctctatat acaataatgc ccaaacagaa atatgagaac gagcttaatt ctaattataa
     3841 tgtccatttt actaatctga aagaacctcc gcaacagaca gaagtaatga tctttcattc
     3901 tggttttgat tctttaacaa ccgttcagct gtataactta gatcagtata ttatgaaagg
     3961 aggtaaacta gttgttttag cagataaaat ggcttcggat ggcgaaagag ttctggagct
     4021 ggattctaac ctctttgata ttctggaaaa ttatggtatt aaatttagca acgccatagc
     4081 tatggacatt ttttgcgatt ctagacaaat gggtgtggat accaatatct cttttcctgt
     4141 ttatcctgta ttaaggggtt ctcaacatcc catcacacag aatatatcca atattgtaat
     4201 ttatatggga aatggcatta tttttaataa aaagccaggt ttaaagtttc aaactattct
     4261 ggctacttct accagcagcg catttttaga aggtcccgat tatcaactgg atgcaagtat
     4321 gtttttacac cccgatccag aggtttttgt gaatccgcca attcccttgg gagccatagt
     4381 ggaaggcaga atggaaagtt attttgccca taagccagac gccaaacaac ccggttttat
     4441 ttctgaagtt aaagatggta aaattgttgt ttttgcggat agagagctat atattgatcc
     4501 cgataatccg atttacgaga accgctatca cattattctg aatgctgtgg attggttaat
     4561 ggaccgtcca gatatgattg ctattcgttc ccgtcattta cagaaaagca ttttggatat
     4621 tccttattat atgcacaaac ataatctcgt ttggggcgag cctgtaaaaa ccgaacgcag
     4681 aattaaactc ataatcaaga ttgttgccat tgttttacct tctttattgc tggttggtat
     4741 tggatggata agttcaataa tgcaaaaacg caatctgctc ttaaaaaatg aagaagaata
     4801 aactgattct gctgctaatt ctgcttttgc tgattgggtt atactttctt ttgcggacaa
     4861 agcaacccca ggaaaagctt cttcctgttt ttgatctgga tactttagcc atcaatagaa
     4921 ttgaaattta cgatgcccaa gatactctca aaatggttaa acagggtaat tgctggagat
     4981 taatataccc agttaattgg gaagcagata gcctaaaaat tcactcccta ttcaaagata
     5041 ttttaactgc caaatattct aaaactccga tgggaacagg caaagaggca attcttaaat
     5101 tccaccagag agatgaagag acgttacatt taattgttag cgacggcaaa aaaaccgtgc
     5161 ataccctttt tagcaatttg aataatccat acgattattt tcgttatgct gggtcggaag
     5221 agatttatca ggtgaaaact aaagttacca atgtatataa taccgatttg gcaaattggc
     5281 gttcacctca tatcgttagc caccaggaaa atgatctggt taaaattgag gttactcatc
     5341 ccaaaaacaa atacacttta accagaaagg gcttggattg gtattatcag gatgcacatc
     5401 agaattttca aattcatcct tataaccgag ctatgataaa aatcctaaat attctggaac
     5461 acctggatac ttatatattc gtagataatg ctcaagcgga atatgctgct aaatttgatg
     5521 atccctattg cactgtaaaa ttatttttaa ccaataaccg cacacaggat ttgaaattta
     5581 ttgaacacta tcaagggcat tatttaatga tggtagataa tgatccaact gttttgtttg
     5641 tagttgtctg ggatactgtt ttccgtttta ccagacctcc tgatgttttt agaatgcagg
     5701 agctgggctg agtatgctta ctataaaacg aataaaacag ctaaataata atctatacca
     5761 aaaagtatct gagctttggg tggaaacagg tatcagcaat cctgagcgta aagattctct
     5821 ggaaattgtg caacaaaatt tggaaaatac cggaactatg ataattgccg aagaagatga
     5881 aaatctgata ggtgttgttt gggtaactca cgattttcgc cgtctgtata ttcatcatat
     5941 ggcagtaaaa ccaaataagc aaaatcaggg gatcggaacc aaactgatgg aagaatgtgt
     6001 tacaattgct aaagaatttg gttatcaacc taaattggaa gtgagtattg ataatccagc
     6061 tgccaggcat ctatatgaaa aattcggatt taaagaacta actggataca gcgttctaat
     6121 taaaagaaat attctaaccg acttatagtc ccaaagcaaa tttttttcgt gcctaaaagg
     6181 aggttgctat gaaaaaaaga ttactggtgg caacaggcgg aggcgattgt cctggcttaa
     6241 atgcagttat tagagcagtc gtaaaaagag cttcacatga acacaattgg gaagttttag
     6301 gtagcgttga tgcctttaat ggtattctac aagatccaat gcacatcatt gaactcactc
     6361 ctgaacgagt tgctggcatt catgttcagg gaggaacaat tatcggaact actaacaaag
     6421 gaggaccctt tacctggcct gttaaaaatc aagatggaac ttggtctact gtagaccgct
     6481 ctcaggattt tatggatcgt ctccggtatc aaaatatcga ggctttaatc aatatcggag
     6541 gtgatggttc tcaacgcatt tcacataagc ttttcgaaat cggctgtccc attatcggtg
     6601 ttcccaaaac cattgataac gatctctctt ctacagattt tacttttggc tttcaaaccg
     6661 cggtagatgt agccaccgat gcagtagata aacttgttac tactgctgca agtcatcatc
     6721 gtattctaat tcttgaagtt atgggtcgtt atgcaggatg gattgcactt cattctgcaa
     6781 ttgctggagg agcggaagtt tgcttaatac ctgaaattcc ctatagcatc gataaagtta
     6841 tggacgcaat taatcagcgc atagacaaag gtcacgattt tgccaatatt gttatcgctg
     6901 aaggagccag acccatagat ggagaaatga cttatataga aagtgaagaa cctggagcaa
     6961 tgaaaatgaa attagggggt gcaggattac ggctttcaca agaattaaaa gatgctggct
     7021 gcacaattga tattcgcgaa accattttag gccatttaca acgaggtgga acccctaatg
     7081 cttttgaccg aattttggct tctcaattcg gagtaaaggc ttttgaactt gttttagaac
     7141 aaaaatgggg ctctatggta gcataccatc atcccaatat agtagctgta cctttagctg
     7201 aagctatcag aaaatataat tacattgatg tttcttcaga cctggtagca accgcccgcg
     7261 gactgggtat ttctttagga gattgatatt ggatggtctc tctcagataa catattagaa
     7321 acaagggttt aagtattatt gaattgtgtt ttatttatct tttgtaattt ctgattcagt
     7381 tcttccacct gtttctttct ttcatatttt tctgcgagat agtgaaaatg atgttcatca
     7441 tctctttgtg caaatagctt ttccagttct ttttttatag ccacagagct ctccattggg
     7501 cagatgaaat tctgtccgct ttcttttaga agttctgcag cttctccttg cttagggata
     7561 agagctaaaa tagtttttcc tattcttaaa tattcaaaca ccttgcttgt aagggttcct
     7621 ttagggctat cagaatttat taaaagcaac aaggcatcag aagcttgcat aactgccaag
     7681 gcttctttat gtgtaatttg tggagttacc ttcacaattt cttcaattcc gctttgctgg
     7741 atttcctgat atgtttctct atggtagttt ccatataagt taaagctaaa accaacaggc
     7801 aatttatttt cctgtttcag attttttaat gctcttagca accattttaa agaacgctta
     7861 gctgtaatgg ttccaaaata gcttaaagta tattgatcgt gatttgtttc catcttgggt
     7921 aaggtagaaa agtcttcttc atcgtggccg ttatagagaa cgaaggtttt gctttgtaac
     7981 tccttattaa aatggttact aatatcttct gcgatgcctt tggtggcagt aacaattaag
     8041 gctgcatctt ttagaatcct tttttcatga tgttgggaat gaagccgatt tagataagtt
     8101 ccctgcagat tatagtcatt tagcaaagtc caataatcgc gcatatccaa aacataaggt
     8161 aacttaaagt gtttacttaa atatcttgca gccacagcag aagaaaaagg tccacaggaa
     8221 acatagatta ggttgtagct atctcccgca agggtttttt tagctgtctt tagtaaattt
     8281 ggcagccaaa gcactttatc gtccaaagga taaagatggc ggataaagag tttaattctc
     8341 tctggagttt gatgatatat gttatttgac atcgttttgt tttttccgct tagcttttcc
     8401 aagatagaca tcggatcaaa tgacggaata tggataatgc gattttgttc gcatttagct
     8461 aacaatccgt tatcgtagaa gttataaaca atattgccca cggttaatac atcaattgta
     8521 ttacccagtt ggcttaaata cttcaccgtt ttcaggtttc gtaaagaggc aggtcccgcc
     8581 agagggggat agaaataact tatatagagt attttcattt agagttaagt ccatttatta
     8641 ttttaaaaag ctctggttcc agcgcttccc aattaaattt gttctcagta agttttatgc
     8701 acttatcgct cattgcctgg tattcgcttt gtttaagatt taacaattta gcacaggcat
     8761 cggctaatgc tttagattga taagccgaac agatacctaa accgtttttt tcaatcagtg
     8821 ctttcattat gggggttttg atagttaata ctggaagccc ggcagccaga tattccaaaa
     8881 cttttagggg ggtggaaagt tgcaagcgct tatttttagg attaaatatc caaagcccga
     8941 atttactgcg ttttagcaaa agaccgattt tttctgccgg aatccagctt tgataataaa
     9001 ttaacgcatt caagttataa tcattgatgc tttgattgaa tctctgttcc acttcctttt
     9061 tgaagaattt gcccacaatc agcactttta gaaaaggaaa atcttgtttt aggatggaaa
     9121 ccattttcaa gattttaaat actcctctgt cttccgttaa tccaccaata tagataaaat
     9181 caaagttcat ctcacatatt tgttccaaag agcccggctt attgcattcg taatcccaaa
     9241 cattttttac cggataattg ggtaaaacta tagaggttat gcgtttattt ccacttagca
     9301 attgtttttc catttctctg tttacggtaa aaactgcatc ggctattttc agcaactgct
     9361 ttaaagatac ttgataaaaa agataagcag gaaaacgcaa aaaacgggga aacctttcac
     9421 tgaaggcaat cgcaaagaat tcgtgaacat cgaaaataac ctttagctgc agtttatgct
     9481 tcaaaaccca ggcaatcagc aaagttaagg gctccacaca aattaggata tcaggtttta
     9541 aatccctaac ctttttaccc aattgccaaa tggcagccca acgggaggaa gcgtcaacaa
     9601 ccatttgata gggattgagc ttgttattta ttactccgtt ggtagtaatc aaataaactt
     9661 cattctcttt ggctaaactc attgccagct tgtaatataa gcgcacatca ttcgtaggat
     9721 gcgtgttggt aacaatacat atcttcattt tcaggactgc ctttttaggt taatctttta
     9781 cattatatta aagcataagc atttatgtca agaggaaaag tggggttatt gcatcgataa
     9841 cttatttaag cataccaaaa caaaagagca taccgcgccc cataatttgg ctctgaaagg
     9901 atggataaat aaaatacgct aactctcaat tattgaacaa tgctatatcc cagtttccca
     9961 aatgctaatg cgaggtttgg gagattggct cgtaagaatc tattatatcc atttctacgc
    10021 ctgccagcca taacttaatg aggcaaaaag aattctgatc aggagttgta ttatattttc
    10081 cgcgggcatc ttttagtttt aacccatcat tttttgtatt ttcatacaag atagttagac
    10141 gctcgggata gagcattcta gccacaaagc gtgttttagc gcggatatcg ctaagataag
    10201 aatatataca atgatgcatt tccattcctt ctctgtataa ttctatggaa ttatgaatat
    10261 gctctatcca gagcgtatgg catttaatat ctttggcaat gccttccggt aacggcacaa
    10321 agtctatctt ttttaatttc aatatttgta ttaccagttc atcgtggtaa gtattcaaat
    10381 cagttatgtt tttcagtatc acttttctat cctgtagccc gttttctctc aaaacttcaa
    10441 gcatccggta tatatcattt atttgagagt ttatattcaa gacctcctct tgattcatat
    10501 tggcaatttc agcgattaga ttgtagtttt gcattatatc taatcttttg catacaagaa
    10561 ataaactata atcattatgt acagggaaat aagaaagtgt acgcagtaag ttaggattat
    10621 tctgcaaact ggttcttaag ataaagaaaa tattagtgga acaagattct ggttttatct
    10681 tggcaaaggt cttaaccatt gtttcgcaag ctggaaaacc aaaatagccc agaatatctt
    10741 tgcgtttttt ggtaatcagt ttggtagaag attgccagta ttttttactt tttagaggat
    10801 gaaaaactgc atatgcactt agcaaataac caattgccgg atttgtttgc atcagttcaa
    10861 gcgcataatt tcctcctctg aagagcagac ataaaataaa ccaaaagcgg gaagtaaaac
    10921 cattcagcag ttcaattttt tccgctccta tataagcttc catactttta tagatagatt
    10981 ctttgtgtag agcattcaaa cgaatagcgt aattattgct ttttagattc tcgggttttg
    11041 gcggataaaa tagaaaagga aagggttggg taggaagaaa acactcccaa taattgtctt
    11101 ttagcttttt ttgataagca gcaggatagg gatttcccaa acgaataacc tttatatagc
    11161 gtttggtaaa atgatagagt tttccctctt cttctttaaa gattattgct cttttttcta
    11221 tttccttata tggtctgcca gtagattgaa tatcctttga aaacatagat cgcctcctat
    11281 cttttttcat agccttgaat ttatcagcat ttcaagaaat cggataagtt cattgggtaa
    11341 atagataact atactgtcaa gaaaaaacag aagtgagaaa taaggatatt ttccttgacg
    11401 aaaccccagc aaataaaata gttgagaaaa aatatcaagg agacgcacaa tggctgtcaa
    11461 gatcgataaa gaaacttgtc tcggatgtgg tgcctgcgtt gatggatgtc cagtttctgc
    11521 tttaaaaatg gtagatggca aagcagaatg tgatgcatca ctttgcatag attgcggtgc
    11581 ctgtattgct acttgtccag ttcaggcaat ttcagaataa tctaagtaat aatagtttag
    11641 ctaaaacgct gtggcaaaat tggaatttga tttctttttt gccacagtgg atggcttata
    11701 ttgtcaattt atcctcttca aggatagaat agtagttcgg aaactcatac attcattaag
    11761 ttgctgcttt ggcaattttt tctttgtaag taatgaggaa atcaattatc ttataaatat
    11821 gtttcatttc catcagatca aatttgatta atacaaaacc caaaaggagg tgaaaagatg
    11881 aaacgcttaa gtatatttct tggaataata acctttatat cactttgcta ttccattcaa
    11941 gtgagtggaa atcaaagtgg tatatggtct ccggaaaata atccatacga agtgataggt
    12001 gctataactg ttccttccac agattcttta atcattcagg caggggtgga agttcatata
    12061 acaggttcct atcaaataac tgttcaagga attttgaatg cttatggaac agaggcagac
    12121 agcattcgtt ttttgaatat gcaaacatat cctaccgctc tttggcctgg tttgcgtttt
    12181 gaaaatttac agcttaccag tcacttaaat tatgtctata tagaatatgc cacttacgga
    12241 gtgcgcacga taaattcacc cattgccata tctcactgtc atctaaatct ttgtgaaaag
    12301 ggaatagagc tttatggcat cggttctgct attccattac caaatgtagt agagtattca
    12361 ttaattgaaa attgcacgca aaacgctatt ttggttacct ctaatagcaa tgcttatatc
    12421 aggtataacg aaattcgcta taatggaacc ggacctcaat ttcgagcggc gattcaaatg
    12481 ggcaatcaat ctggcgtaaa tcaatgcaat cccaatatct taaataatca tattcatcac
    12541 aactacaagc agggaatttc tgcctgggac attgcttctt cgggttccat caatccccag
    12601 attttaaata acctcattga atataactat accggcatct atttattaca ggcatcaggt
    12661 tatgtagcgg ataatcaaat caacttcaat tttattccgg gagatatgaa ttccggagcg
    12721 ggagtaatgg tttcgggagt tacatccatc ccttattttg agcgcaacca tattgagggt
    12781 aattataccg gattttacat tactaataat ggtaaaccag ttttaggaga cctggctata
    12841 gatcatatct gggcgcaggg agaaaacacc attatcaaca atatcgatgc ttctggcaat
    12901 aataattctg ttttttgcga tgcctatgct aatgccagct ttattattat ggcagaaaac
    12961 aatttttggg gcttttcttc ggcagccgaa attgctgaga ccatcaacga ccacaatgat
    13021 aatgctgctt tgccaacagt agattttgag cccttcataa tccctgtgga aaatactgcc
    13081 atcacaggca attatactta taatggcaca gctcaaattg ctgcatcacg cttggaacta
    13141 attaccgtgt ccgaaagtaa tattcaagtt acagttcccc tttccgagct tactttttcc
    13201 cttgaaacag atatcgctga ggatttttat gtgcaggttg ttctaaccga aacagcaact
    13261 ggcaaagatt tttatggctg tgccggaggt tatagcaatc ctactgtttt ttcacccaat
    13321 ccaggaaatt ccattaatgt aggcacaatt gaagttaccg atagtcctct gccccgttat
    13381 gaatatgtgg cagaaccatt tcaggaggat aatttaaccc tctatccttt ggcaacggga
    13441 agaggacttt acagttatga caagctggat atcgtttatg ctgaaggtga ctatctatac
    13501 ctgaaaagaa acttttggca aactcccacc ggggaagtgg taacggaact acctgtaggt
    13561 actatttaca aaaaatattt gaatattaac gccggggata gctggcagca gaccgaagtt
    13621 atctatccgg gcaatgtaac ttttacttct accgtttatg tggataattg cgggggagaa
    13681 ttttccaatt ctgacaaact ttttacgcgt aaggacgccc aaggcaacat tgttgataaa
    13741 tggttgaaat tggatagcgg agaattactt ttccactatc aaaatcatta taccattgct
    13801 aaggaaaggg ttcagtatat tcccggcaat aatgatccct taactgccgg caatattact
    13861 ctctttatcg ctgaaccgtt aaatgataat cccacttact tggtttatga tcccaatatt
    13921 caaaatcagg ttcgcctttt ctggcaggca ccttccacgg gaaattacag ctggacaaat
    13981 tatcgtatct atgaaaatga tcagcaaatt gccgaaattc ccttctctct tagcgaatat
    14041 gtggttagtc cttttaatcc gcaagtgaca acttcttatt acataaccgc caccgatgga
    14101 accaatgaat cggaacccag caattcagtt acggttatta tcatcggaac cgaagatccc
    14161 attcagaaac cggtggtgat caatgttttc cccaatccag taaattttgt gaccggctct
    14221 gctttgaaga tagaaatgaa gaacttgcag agtagaaatg ccgagctgga aatctacaat
    14281 attaaaggac aattggtttt ccgtcaaaac aattttgagg ctaacactat cctctggaat
    14341 ggaatagata ataaaggtat gcgcagcgct tccggaatct atttcttgaa agtgaaagtt
    14401 actggcgaca aacctgtaac ccgtaaaata ttggttctat aaattagttc cgaactttca
    14461 gctataccag attcgggata atacatcgct ggttattgct gaaatagcag gaattcacca
    14521 aagctatata cggtgaatct atatttgaag agtgaaaata gcataatata agggtggtag
    14581 atgttacatc tgccaccctt ttcttattca tcgctttatc ttttttccct tatccttgct
    14641 tagttagctg tgcaggtact gcaatggtga ctgttgggat tttgccgagt agctccacga
    14701 cagttttacc acaactaatt aactagggac tgcagacagt aaaaacaaaa tgagaattgt
    14761 tctcaccggg ttaaaaccca ttgctcaaat cagtaggtct gctgggactt ttacttatta
    14821 aattatccca taaatagaat ctgtgttatc tgcaagaaac atctttttct tagttatctg
    14881 cgggaaacct atctatgagc tactttttat tcaggataca tatacacaga aaggcaggat
    14941 ttatcaaagc cgaaatattg ttttgcagct tccataatag ccttcttatt tacattatca
    15001 taaagagcag gataatccat aaagcaatca atcggcaagc ccagagacac attactgctc
    15061 atattattga tccagtattt attggtattg atgttttcct cgtaggtttt atgcagagta
    15121 gttcttgtgg catcgagata tttatcagca aaaagtccat ttttcaagct atccagggta
    15181 gcaaaggtag cagcattcaa atctttggct cttatttgat cgcaacccat ccaagtttga
    15241 gtaacaaaac tcagtttagg aagaaaatca taactactcc agacggtaac ggcataaact
    15301 ccgctcatat tttcgcgaac attttcgcgc aatttatcgt tagccagtat catcaacgca
    15361 ttttgattaa ccttggtttg aggggaaaca gatgtcttac caactgtaac attggaaaca
    15421 aagctgcgat ctgacccctt ggtaaaaaca atatcctttt tccctgtaaa agttcttacg
    15481 ccaacatcag ttcttttttc tattctacct tttgctggta aattggctaa atatgttcta
    15541 caatagtttt ttagctgctc ttcctcaaaa ttacccacta tataaaaggt gaaatcggag
    15601 taatcgctaa agcggtcttt atagatatct tcaatttgct tcagagttaa tgtatccaga
    15661 tcttccggat gtaactgtct ttttatagga tggttttcat agataagagc atccaaagta
    15721 tcaaaaaaag cagtactggg atccagaaga taattttgcg tccaggtttt cattgaagct
    15781 acggtagaag aaaatgctgc ttcattataa cgaggggcat aattatattg atacagcatc
    15841 tgaaacatta gttccatatc ctgaggtgaa cagctacctc tccaaccttc tgaataattg
    15901 gatatagttg gatagacatc agcaattttg ccagccatag ctttcttcaa ggcatttcca
    15961 tcaaaattgc caaaaccagc tacataaaaa tattgattta gaagatcagc tgcttggtaa
    16021 ttttccggtt tcagtttggc tttgccacca gggctctgag ctatcattat tacttcatct
    16081 gccttaaaat ctgtcttttt actgtaaacc tgtataccat tggaaaggac ccactgttta
    16141 atttcggatt tgggataaac cttttctttg gttattttac ctggattagg aatggtttct
    16201 aaaaggggtt cattaactgt tatatcttcc cagggttcca attcttgagc agttgcctga
    16261 tgataaatat tcagtaaatc atccttactt ggatatttta ctccttcttt tgctgttcct
    16321 gctaagctta aagtgagatt ctcggaagta atgacttcat caaccatttc atttacttcg
    16381 cttaaggcaa tctcattaat caaacctttc agtaattgtt catattgttc aaagctaaga
    16441 attgtatttc cagagtttgc atcatccaaa atttcccaaa ccagatcttc cgaattcatt
    16501 gtctttttct ccgcaactct ttgttccgct tctctaatca ttatttgttt ggctcgatct
    16561 aattctccag gttgaaaacc gtgctgacga acccgagcta attctgtgat caaggttcta
    16621 aaggcttctt cactttgccc ctcattagca aacattatac aatcggtggc aacaaaacct
    16681 ttcagccagt tctgattata tatataagca aaagaaaaag gaggattaga ttgatggctt
    16741 agctcttgta aacgcgcatt taccaaagtg aaaaataaat cctgctttat actataataa
    16801 aaggaaccta attctgtaat aggttcagat tttactttcc aagtacttct aagcatcgtg
    16861 tatggcagtt ctttatctaa aaccgttata gctcgcggtt ctatattgtc tggaactgaa
    16921 taatgaactt tggggcgagg attttcccgc ttggggatag cgccaaaata ttctttaatt
    16981 acgttttcca ttttcttggg atcacaatcg cctacgatga taactgtttc taaatcggga
    17041 cgataccaat cctgataaaa ccgaattaag ctattatgct tgaaactttt caagttttca
    17101 attgttccga tggggttacg ctcagcataa cgagaacctg caaaacgcac tttatctatc
    17161 tgatcctgaa ttcgttgttg cgcgctttgt cccaatctcc attcttcttg aataattcct
    17221 ctttcacgct caatttcact gggctcaaag cttaactgcc aggcaatatc tgataaaatg
    17281 gatatccctt ttaacagttt agcctcatca tcggtgggga gtttaaattg gtaaactgtg
    17341 gagttataac ttgttccacc atttaaccca ttgtgaaaac ccattccaat agaagttaaa
    17401 taatctacca tttcagttct gggaaaattc ttgctaccat taaaagccat atgctctaca
    17461 aaatgagcca gacctctttg atcatcatct tcatcaacac tgccagcatc aatacaaagc
    17521 cgaagttcaa ttcgcttttc aggtttggca ttttgcttga tgtaatattt taagccatta
    17581 tccaatattc cagttattac ttccggatcc tgctgcaagg gtaaagctga atattcagga
    17641 ggaaggattg cccctaaaga aaagaggcat ccaatcagga tcaagctgag gactattttt
    17701 ttcatttgta ctccctgtta attatactct gatctatatg taattaaaaa ctgttcatct
    17761 ttgtctaaaa tggtattgta gcttccaaag gtctgatgag ttccatcgga aagataaatc
    17821 tccacttgat aataataatc cgttccatat tctacgcata aaaattcgtt ttcaccaggc
    17881 ataatatttg tcaatacccc tacttttaaa tgggttaaat cactatgctg ccaaatttct
    17941 gcccgactaa tagtttgctc cgatgcattt actattttca cgctggctct attgggtaaa
    18001 aggaaaattt tataaatctc ccctgcctgc acttcaatcc aggtagaatc aagataagca
    18061 ttaagatcct tatcatgaat actataggtt tctcctttca gccaaattaa cagcttcttt
    18121 tttacatcac cggcaaatat atattgagtt ttggtatcta tattatattc aacttcttca
    18181 tctgcgccaa tagttttgaa ttcttgccct tcaactttta tataagctgg ataggaacaa
    18241 cgattataaa cttttagctt tgcccctgat tcacaagcgg aaagcattaa aagaataatc
    18301 gcgaagctaa ggataatcca tattctattt ttcattattc tttcctccct tcttaaaagt
    18361 tatccctttt atcagactct aactgtcaag taaattgttg gttgaccaga tccacactag
    18421 ttttttataa tcggtaggat taattgctga tatcttttcc ctctttgaaa gcccttttgg
    18481 gcaatacaaa aatagcgaag tgacgtaagc cattttcaaa aattcacaat gcagacggca
    18541 aaaggttttg gcttacaatt ttcacctaca ttacccaaaa ccatatccca cgaacttttt
    18601 actaacaacc aagctataac tggtttccga attgagaaat tgtgagttaa aacagagtat
    18661 ttttctaaaa gatttttcct aaaagagtgg atgctaaaga gataaatata tccctaaggt
    18721 attcattcct atatgataac tataagtgtc aattacaatt ggaaggatta aag
//
LOCUS       DAOSTH010000025        18496 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3152_length_18496_cov_12.314408, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000025 DAOSTH010000000
VERSION     DAOSTH010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 18496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18496
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3152_length_18496_cov_12.314408"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1544..3436)
                     /locus_tag="PLB65_04660"
     CDS             complement(1544..3436)
                     /locus_tag="PLB65_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77854.1"
                     /translation="MKKTLLTLILAGVLLFAFAVPRNLVVVEIATGTWCPYCPGAAMG
                     ADDLITNSNPAAIIENHNGDNYANTYSNARNSYYGISGFPTAMFDGLNPVEGGSNTQS
                     MYPQYLTRVNARMAIPSKYTISATGSITGNTLSITANVAKPEDDSNSNIVLHCVITQS
                     HIQHNWQGQTHLNFVNRLMIPNQNGTPVSLATGGQQTYNLSGTFDPSWNINSCEAVLF
                     LQCNTTKEILQGVKYSLPGLLGSNPVSATELNFPDTYVSGHSTLDITLNNFFEFEVTG
                     TISSSNPVFTPIPATFTIQPYQSTTISVEFAPAAATNYTGTLTIDSNLSGYNQVQIPL
                     TGTGFNNTAPNITNIQISGPPVVYQQLSVSYDFADPDGDTEGETEFQWVQIINNQPVD
                     IEDATEQTYTIDIADIGWQLACRVTPKDQYGMPGTPVFSNYTLPIEELPPPQNLTATV
                     TAPNTVTLHWEKPNHFDNRGFVGYRIFRNSFILNNVMNVNTLEYVDTEIPDGTYEYWV
                     CSIFNSPYSVSNPSNSVTVIINLPNSDELAPALEGVNVYPNPFKENTTFQINGKANRP
                     ITVEIYNLKGQLIHRLNSVIDQNGSTQLSWNALDTLSKPIPTGIYFYRVKSEGISQNG
                     KLIKVR"
     gene            complement(3478..4629)
                     /locus_tag="PLB65_04665"
     CDS             complement(3478..4629)
                     /locus_tag="PLB65_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOQ77855.1"
                     /translation="MNDRINSIFKEIKAEHESILGKRAFYNSNAWRNKKEEPDIMRSQ
                     FAVDRDRILYSGAYRRYHGKTQVFSFTNMIDEEMTNRNLHIAYVSQISRTIGKYLKLN
                     TELIEAIALGHDLGHCPFGHDGESALSRCCEQNDIGQFHHNIESLHIVDHISRKGKGL
                     NLTFQVRDGIISHDGEVHNTQLIPNTAKKEADIQSYITAKKIGEDTEFMPATLEGCVV
                     RITDTIAYIGQDIEDAIRFNILRRDELPKEQAVYLGNTNSQIIDTLIKSVIVNSFDED
                     FIAFDTETSEHLLALKKFNYRRIYTDEKVKKSNSIIYRTMPILFDIYLNDIKENNRES
                     KIFKHFLDHKNPEYLDNYSPAERVRDFIATMTDRYYNEEIKAYLLPWKG"
     gene            4980..5228
                     /locus_tag="PLB65_04670"
     CDS             4980..5228
                     /locus_tag="PLB65_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77856.1"
                     /translation="MIYTLPDGRQVDLSKVESISKIRDYGQVDNIVQRYLIGFSIRLM
                     NKEIIEVKDIYHYSDWSEVKLRMNKLREDIINKWKSMQ"
     gene            complement(5530..7956)
                     /locus_tag="PLB65_04675"
     CDS             complement(5530..7956)
                     /locus_tag="PLB65_04675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77857.1"
                     /translation="MQVKSIKLLSIIMITLSAILLLSGCGKSGSRFANQPPTIRITSF
                     EGWDSTYVTAGYDTSLVYTFQQRIYWHATDPDGIITGYAFRVLDENNNPIPTPGYDYI
                     DTTGELTPDNLLAFGTGWVIHYMQGADHNIPLDDPSARRTIWTSQKYAVINFPSADAY
                     GNPIDKSSRFEVVAIDNRGAITPQPAWRNFCTHSDRPTCSITTTKGNPNNQEVGSGLK
                     LSFAMKDTDPFITPVPYQYEFQMMKVDSQGNVIPGTESDWYSTISDDNRTDKINEYLL
                     TKHTTPYLTYDYDEQTGQPLQQRTRVTARVTDLAGVVSIPDTNTVINFRVRPGFRPES
                     IIYPTKTYALTNHHFEDYGDDTTPELTVLPRTITQGSIHYATPLFKDFEGKYTAVYSP
                     NIKLWVRWGWWGEYGDVRDNSADYGDTTNLIAMPYGKKVDVVLDSKTRKNYFSEITAF
                     DIRFDGEPYNYPPFPFSQNGVTDQDGKRWLRIPTNSPLGQTVVLTSGQLPAPVGTGPG
                     KHTFEVRVVDLQDEYDHTPAVFTFYLHPYIEPANRNGVLIVDDDVDSPNQAPDALIDE
                     KYANMLSDYTGPVDVYSRRTHVKGKINAEEDIRGRSLAFSDLQKYKLVIYHSDNINDT
                     GNLLYELDGLSLFMMKGGNMVISHSHRLSPILSEIADSGTRPTFVTNLGFESNPKIGF
                     LNDNNRSAFFQNAVACMAGYENMHLQYGTPPSFLNMVNLRHGLGTLSYFEEGHYTGEA
                     IYKFGCKPVGYEIYPPTNDQYNFYNGKTVAFRKVNPSNSRAYVFGFPLSLMKDSETKA
                     MMNKILSEVM"
     gene            complement(7988..8902)
                     /locus_tag="PLB65_04680"
     CDS             complement(7988..8902)
                     /locus_tag="PLB65_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HOQ77858.1"
                     /translation="MEKILSGKPVAQLVNKATKTLITQYELKPTMLLIQVGSDPASNY
                     YVQNIINSGKKLQCNCEVIDLPEQITEQELLAIMEGANQNPDINGIMLQKPLPSHIKD
                     NIINMAIDPGKDLDCLHPLNLGKILSEENGFLPCTPAAVLFCMQYYGINPQGKNAVIL
                     GRSNVVGKPLANMLLWKKSFANATVIVCHSKTNNIAEITQKADILIAAIGKANFVTPE
                     MIKENAVILDVGINEEKGNDGQSIYVGDVNYNLCFDKASAITPVPGGIGRITTSILYF
                     NLIKACLEQKNINKSIDEYLDLIFKGIH"
     gene            complement(8908..9717)
                     /locus_tag="PLB65_04685"
     CDS             complement(8908..9717)
                     /locus_tag="PLB65_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HOQ77859.1"
                     /translation="MKVLFFGDVYGKAGRRTVQQALPQLREEFNPDFVLANAENLADG
                     KGLTEKTLSQLFNSGVDAVTGGNHLWDRTESLEYLHSQKRIVKPLNYSEEAPGNSYLV
                     LSKGDLHLGIICLCGQLLMPPCDSPFAALDRFIKYSLSIDNEKQNNIPLPYPIILDFH
                     AETTSEKRAMGWFADGKISAVLGTHTHIQTADEEILPLGTAYITDVGMTGSHNSVIGV
                     KKEIIIEKFRTSVPNRFESSDEGLQVNAVFLVLDSETGFAEKITRIKRNID"
     gene            complement(9717..11285)
                     /gene="rny"
                     /locus_tag="PLB65_04690"
     CDS             complement(9717..11285)
                     /gene="rny"
                     /locus_tag="PLB65_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HOQ77860.1"
                     /translation="MNINPYIALVIGVAVGFVIALFIYIIKRNSTFKLISQSTKIAEE
                     LERNAKIEAENAKKAALLEAREEWFKQKRILDEEIKERQKELRTQEKKYNERLSSLDK
                     RLDSLDKKETNLTEQERKLKQKEEELANKKTEYEQIITEQKNKLVEIAGLSRDEAIAL
                     LREELISQARQVAANDARLTLEQIKLDANQKAAEILSTAIQRIAVDHVSETTVSVISL
                     PSDEMKGRIIGREGRNIRTFEKVSGVDLIIDDTPEAVVLSCFDPVRREIARLSLEKLI
                     ADGRIHPGRIEEVVAKTTKEMEENLVKIGEKAVLETNIHNISPNLIKVLGRLHYRTSY
                     GQNVLQHSIESAWICGIIAAELGLDQELARRAGLLHDIGKAIDHEFDGSHAIIGANLA
                     RKNGEGKIIVNAIEAHHEEVEAISVYAALTQASDAISGARPGARREMLETYMQRLSKL
                     EEIANSVEGVSKSYAIQAGRELRIIVEPALVDENQTTLLATEIAASIEKQVQYPGQIK
                     VTVIRETRQIAMAK"
     gene            complement(11518..11781)
                     /locus_tag="PLB65_04695"
     CDS             complement(11518..11781)
                     /locus_tag="PLB65_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOQ77861.1"
                     /translation="MKSVEVEIFGRRFRLRSDNPERTKHIAEEIDRQIEDLSKLYDNL
                     DFTKLLLLICLQQQEEVLTLGDKNQSLMSELERLNQMLSKITI"
     gene            complement(11784..12110)
                     /locus_tag="PLB65_04700"
     CDS             complement(11784..12110)
                     /locus_tag="PLB65_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77862.1"
                     /translation="MMESNVTNLQDKIQKLIDQYTAVTQKVEELEEINKQLAEENSQF
                     IAQLQNRDVATGEQEKKYKTLQDEYNILENRYKELQRILAGFETVAEGAIKKIDGILP
                     PLDNEK"
     gene            complement(12113..14659)
                     /gene="pheT"
                     /locus_tag="PLB65_04705"
     CDS             complement(12113..14659)
                     /gene="pheT"
                     /locus_tag="PLB65_04705"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOQ77863.1"
                     /translation="MKISISWLAKYLEIKENTKELEELLTFSGIEVEAVQNIPALPET
                     VFSAKVISAEPVPKTDHLKLCLVDIGNYPYPEKTPEGYLQVICGAPNCKTGMMAVIAL
                     PGSTLADITIKKAKIKGIESNGMLCSEKELGISDNHSGIIELEPDTPIGRSVNELYEL
                     PDTVFELEITPNRPDLLGYIGIARDLSAKLGRPLKLPEIDIPVAKCRAETMPLELINK
                     VPELCPRYIARLFDKVKVKESPLWLKSALIKSGLRPINNLVDITNYVMLEYGHPLHCF
                     DYEKLASLPDKPGIPAIVIRQAYKDEPITTLDGKNYILEGEEMVIADGIKPSAIAGIM
                     GGNISAISENTKRIVLESAAFDPRSIRRTSYKLKISSDSSYRFERHLSECSAQDASLR
                     ASQFICELADAELCEVVYDNYPVPEKSLILGVRPSRYAKVIGYELEEEKIKDYLQRLG
                     LKFLRYGIWKPGKITNISEIRELDSETLKQNNTALQNQEKAHYYLIPPKRVDLEREID
                     IIEEIARLDGYDKAPVKTKPQHIMDRHSYRIKKVAVDYLVSRGFYETLNYSFSEPELM
                     RKLGYQEGDYELDMIFLKNPQSSNQSAMRTSLIPQLLQNLAYNLNRDEKNIKLFELGK
                     TYHRQETISYEPYYLAAILTGLNKDEHWQDKASPINVFWVKGIVEELLSLLHLENWTV
                     QEAKKPYLSQSESAAYYLDNKELAYYGKLTAPVAENFDIDLIELKQDIWIVQFSMENL
                     IVATHNLEIQYQEIPRLPSVIRDISFLISEEIPYSKIVKTIAEVDDKMITDIIAFDEY
                     RGKQIPNGKRSLTLRLKIRDKEKTLTDERVDYLVDSVIKKLENTWKITMR"
     gene            complement(14656..15690)
                     /gene="pheS"
                     /locus_tag="PLB65_04710"
     CDS             complement(14656..15690)
                     /gene="pheS"
                     /locus_tag="PLB65_04710"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOQ77864.1"
                     /translation="MEEELKELVKQAESDILACASYNDLLNIKAKYLGKKSVLNSLYA
                     KMRDIPSEERPAFGNLINATRKNIEDLLEKQNKAIKEDAWKKSENRTAIDLTMPGIFS
                     SRGGLHPLTIIRREIDEVFLGLGFEIAEGPDIDDDFHNFDALNTPPDHPSRNLADTFY
                     LEGGGLLRTHTSTVQVRVMEKYPPPIKIISPGCCYRNDKPDPSHSPVFHQVEALVVDK
                     GISLSDMKDMLSQFASIMFGKNVSSRIRPHFFPFTEPSAEMDISCVVCGGTGCRVCKY
                     SGWIEMGGAGMVDPNVFDILGIDSEIYTGYAFGLGIERIAMLKYNIPDLRILYENDLR
                     MLRQFKGELR"
     gene            complement(15936..16283)
                     /gene="rplT"
                     /locus_tag="PLB65_04715"
     CDS             complement(15936..16283)
                     /gene="rplT"
                     /locus_tag="PLB65_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOQ77865.1"
                     /translation="MPRITNNVAAHRRRKKYMLNARGYFGNRSKTYRATRQTVERAMV
                     FSFAHRKLKKRQYRSLWITRINAACRINDMSYSQFINGLHKANIQINRKTLAHLAWHD
                     APAFAKLVEIAKG"
     gene            complement(16300..16494)
                     /gene="rpmI"
                     /locus_tag="PLB65_04720"
     CDS             complement(16300..16494)
                     /gene="rpmI"
                     /locus_tag="PLB65_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007840008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOQ77866.1"
                     /translation="MPKLKTNRSAAKRFKVTGTGKIVRHHAKSAHIKTKKSPKLKRNL
                     RTSAIVKKCDEKKINRMLGL"
     gene            complement(16586..17281)
                     /gene="infC"
                     /locus_tag="PLB65_04725"
     CDS             complement(16586..17281)
                     /gene="infC"
                     /locus_tag="PLB65_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOQ77867.1"
                     /translation="MKQAVFSIKKIAYFITGKKEVLIIRIKKGKARTNEVVPKERINN
                     QITADKVRLIGPDGKQIGIVSLKEALRKANDMELDLVEISPNAEPPVCRILDFSKYYF
                     EKEKKAREARKRQHEVEVKEIKFGPNTEEHDYNFKKNNAIKFLKQHNKVKFTVRFRGR
                     QMAHKELGYKVLDKLKEDLAHLIEIDSEPVSERNMLSIVVSPKKDIDRILAKEAKKEE
                     MPASDSEKTDPQA"
     gene            18237..>18496
                     /locus_tag="PLB65_04730"
     CDS             18237..>18496
                     /locus_tag="PLB65_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1848 family protein"
                     /protein_id="HOQ77868.1"
                     /translation="MQISDGISFPDIKKQEPIIISASRATDIPAFYTDWLISRIEAGY
                     IKWKNPFNGQIKDVSFAKTRLFVFWSKNPSPMLRKLSYFDEKG"
BASE COUNT         5472 a         3417 c         3454 g         6153 t
ORIGIN      
        1 atagaaattg aattgacccg ttagagggga ttgagacttt aaaggtgcta acggttaatg
       61 cggtcagctc gattgtatag aaattgaatt gacccgttag aggggattga gactttgcct
      121 tcaggtaatt tgcccttgag ggttttaaat atatagaaat tgaattgacc cgtttgaggg
      181 gatgcctctc acagatttca cagattacac agataaagat ttcacgcaga ttacgcagat
      241 gacgcagatt tgaataggca agggatgaac aggatttaag ggatttatag gatttcgaaa
      301 ataagtccca gcgggacgat agatattaac gacgggtttt aacccggcga caagaagaac
      361 ggataaaaaa gtcccagagg gacgacagat attaacgacg ggttttaacc cggcgacaaa
      421 atcgctggaa ggttaatgtc cacatcaacc aaaaaagaat aggtaaggga tgaacaggat
      481 ttaagggatt tataggattt tagattataa ttaaataaac tggttttctg atttttatat
      541 atggaaatgg aactttaaaa tgcaccagtt ttggcaatgg aaaatgggac aaagataaaa
      601 ttgacaggaa tagtgtgttt gtataacttg aatgtatcct aaaaagttga ggtggggatt
      661 aacttcaaga cctctcgacc ccttgacctc tcgacttaga tagacccgtt tgaggggatg
      721 cctctcacag atttcacaga ttacacagat aaagatttca cgcagattac gcagatgacg
      781 cagatttgaa taggcaaggg atgaacagga tttaagggat ttataggatt tcgaaaataa
      841 gtcccagcgg gacgatagat attaacgacg ggttttaacc cggcgacaag aagaacggat
      901 aaaaaagtcc cagagggacg acagatatta acgacgggtt ttaacccggc gacaaaatcg
      961 ctggaaggtt aatgtccaca tcaaccaaaa aagaataggt aagggatgaa caggatttaa
     1021 gggatttata ggattttaga ttataattaa ataaactggt tttctgattt ttatatatgg
     1081 aaatggaact ttaaaatgca ccagttttgg caatggaaaa tgggacaaag ataaaattga
     1141 caggaatagt gtgtttgtat aacttgaatg tatcctaaaa agttgaggtg gggattaact
     1201 tcaagacctc tcgacccctt gacctctcga cttagataga cccgtttgag gggatgcctc
     1261 tcacagattt cacagataac acagataaat gtctcacgca gattacgcag atgacgcaga
     1321 tttgatttta gaaacaagga ttttaggatt atagttttag aaacaaggat ttcaggattt
     1381 caggattaag aggattattt tagctgaggg ctgagggctg agggcagagg ctgagggcag
     1441 ggaagaaatt gagaatttta aactcctgca ctaagaacaa aaaaaactcc catcagccat
     1501 atataccaat gggagttaac agatttgtag tatattttgt taattatcta actttgatta
     1561 gttttccgtt ttggctaata ccttcgcttt tgactctata gaagtaaata ccagtaggga
     1621 taggtttaga taaggtatcc aaagcgttcc aggataattg tgttgagccg ttttgatcga
     1681 taactgaatt gagccggtgg atcagttgcc ctttcagatt ataaatttcc acagtaatgg
     1741 gtctgttagc ttttccatta atctggaaag tagtattttc cttgaaagga tttgggtata
     1801 cattcacacc ttccagggcg ggcgcaagct catcgctatt aggcagattg atgataacgg
     1861 ttacggaatt ggaaggattg gatactgaat aggggctatt gaatattgag catacccaat
     1921 attcataagt tccatctgga atttcggtat ctacatattc caaggtattt acattcatca
     1981 cattattcag gataaagctg ttacggaata tgcgataacc aacaaatcca cggttatcaa
     2041 agtgatttgg cttttcccaa tgaagagtta cagtattggg agcggttacg gttgctgtaa
     2101 gattctgagg tggaggaagt tcttcaatag gtaatgtata attgctaaaa acaggagttc
     2161 caggcatacc atattgatct ttaggggtaa cacggcaagc aagttgccag ccaatatcgg
     2221 caatatcaat agtataagtt tgctcggtgg cgtcttctat atctactggt tgattattaa
     2281 taatttgcac ccattgaaat tcagtttcac cttcagtatc accatcagga tcggcaaaat
     2341 cataactgac ggataattgc tggtatacta ccgggggtcc tgatatctga atattagtaa
     2401 tattgggggc agtattatta aaaccagttc cagtaagcgg aatttgcact tgattataac
     2461 cgctcagatt actatctatg gttaaagttc ctgtatagtt agtagcggca gcgggtgcaa
     2521 attctacaga gatagtagtt gattgatatg gttggatagt aaaagtagca ggaataggtg
     2581 taaaaacagg attggatgaa gaaatagttc cggttacttc aaattcgaag aaattattca
     2641 aagtgatatc cagggtagaa tgtccgctca cataagtatc agggaaatta agttcagtag
     2701 cggaaacagg gttcgatcct aaaagaccag ggagcgagta ttttacacct tgcaatattt
     2761 ctttggtagt gttacattgc aagaaaagaa ctgcttcaca ggagttgata ttccagctgg
     2821 gatcgaaagt tccggataaa ttatatgtct gctgtccgcc tgtagcaagg ctaacaggtg
     2881 taccattttg attggggatc attaatctat taacgaaatt tagatgagtt tgcccctgcc
     2941 agttatgttg aatgtgggat tgagtgataa cgcaatgcaa aactatatta gaattgctat
     3001 catcttccgg tttggcaaca ttagcggtta tgctcaaagt attacctgtg atggaaccag
     3061 ttgcagaaat tgtatatttg gagggaatag ccatacgggc atttactctg gttagatact
     3121 gaggatacat agattgtgta ttgcttccac cttcaacagg attaagccca tcaaacattg
     3181 cggtaggaaa gccgctaatt ccataatatg aattgcgtgc attggaataa gtgttagcat
     3241 aattatcccc attgtggttc tcgataattg ctgcagggtt gctattagtt attaagtcgt
     3301 cagcacccat agctgctcct ggacaatagg gacaccaggt tccggtagca atttctacaa
     3361 ctaccaggtt tcggggaact gcaaaggcaa atagcagaac acccgctaaa atcagtgtta
     3421 acaaggtttt tttcattggt actccttatg tatgtgtgtt ttttttcttg ctttaggtta
     3481 tcccttccaa ggaagaagat atgccttaat ttcttcgttg taatagcggt ctgtcatcgt
     3541 tgcaataaaa tctcgcactc gctctgcggg tgaatagtta tctaagtatt cgggattttt
     3601 atgatccaaa aaatgcttaa atatcttgga ttcgcgattg ttctctttga tgtcgtttag
     3661 gtaaatatca aataatatag gcatagttcg ataaatgatg gaattggatt ttttcacttt
     3721 ctcgtcagta taaattcttc tataattgaa ctttttcaat gctaaaagat gctcactggt
     3781 ttcagtatcg aaggcaataa aatcctcatc gaagctattt acaataacgc ttttaatgag
     3841 agtgtctata atttgactat tagtatttcc caaataaact gcctgctctt tgggtagctc
     3901 atcgcggcga aggatattaa atcgaatggc atcttcaata tcttgtccga tataagcaat
     3961 agtatccgtg atgcgaacta cacatccctc taaagtggca ggcataaatt ctgtatcttc
     4021 acctatcttt ttggctgtaa tatagctttg gatatctgct tcttttttag cagtattagg
     4081 aatcaactgt gtgttatgaa cctctccatc atgtgaaata ataccatccc gaacttgaaa
     4141 agttagattc aagcccttac cttttcggga aatatgatct acaatatgca agctttcgat
     4201 attatgatga aactggccga tgtcattttg ttcgcaacaa cgagataatg cactttcacc
     4261 atcatgacca aaaggacaat gccccaaatc gtgccctaaa gctattgcct caataagctc
     4321 tgtatttaac tttaaatatt taccaatagt gcgcgatatc tgcgaaacat aagctatatg
     4381 caaattgcga ttggtcattt cttcatctat catatttgta aacgaaaaaa cttgggtttt
     4441 tccgtgatag cgacgatatg ctccactata taaaattcta tctctatcca cggcaaattg
     4501 ggagcgcata atatctggct cctctttttt attgcgccag gcattggaat tataaaaagc
     4561 acgcttgcct aaaatgctct cgtgctctgc tttaatctct ttgaagatgc tattaattct
     4621 atcgttcatt aactactcct aattgatata ataattcaag tggggtttac tgtcaagcat
     4681 aacttatata ttttttgttg gggataaatc acacagatta taatttgcct ataaatcata
     4741 ttagcatcaa ggatttttaa tctgaagaag catcgttttt tatgtttgaa tattatttca
     4801 ttcttggtat ctgtctgcag ctatataatt tatcctattt aaagcactat aaaaatagga
     4861 ttagctgcta attactagat attaatatta gatctatatt agttatttta aaaaaaagat
     4921 tgacagcaat atggctgcgg ttataagagg tatattagta tatgattaag gtggaaaaga
     4981 tgatttatac tttaccagat ggcagacaag ttgatttatc gaaggtagaa agcatttcta
     5041 aaattaggga ttacgggcag gttgataata ttgttcaaag gtaccttatt ggtttttcta
     5101 ttcgtttaat gaacaaggaa ataatcgaag taaaagacat ttatcattac agcgactggt
     5161 ctgaagttaa gctacgaatg aataaattga gagaagatat aattaataaa tggaagagta
     5221 tgcaataaaa tcggtaactg attttcgttc ttacggaata tagacctcct agtcaactct
     5281 ttgttaatat tgccttatgg aaaaaatctg cgtgcttaca tatgagtttt gcccttccta
     5341 acaggtggtt tttacggaaa tgaaattgtt tgatttgacc agtctcaaga ccggtgtttt
     5401 atttaattac attgaataat tgtcaagagc agtgtacgag gcctgtatgc aaggttctgt
     5461 gaaagagatg atgctggaaa agatcatcca gcatcatcct actcaattat agtatattgt
     5521 taatcgaaat tacattactt cagatagaat cttattcatc attgccttgg tttcagaatc
     5581 tttcataaga gaaagaggga aaccaaaaac ataggcacgg ctgttactgg gatttacttt
     5641 tcggaaggca acagttttac cattatagaa attgtattga tcatttgtgg gcgggtagat
     5701 ttcatatcca actggtttac aaccaaattt atatatagct tcgccagtat aatgcccttc
     5761 ttcaaagtat gagagagtgc ccagtccatg ccttaaattt accatattta ggaatgaagg
     5821 aggggttcca tattggaggt gcatattttc atatcctgcc atacaggcta cagcgttttg
     5881 gaagaaggca gagcggttat tatcatttaa aaaaccgatt tttggattgc tttcaaatcc
     5941 gagattggta acgaaagttg gtcgtgttcc tgaatcagca atttcagata aaatagggct
     6001 taatctatgt gaatgtgaaa taaccatatt accacctttc atcataaaca agctaagtcc
     6061 atctaattcg tataagagat ttcctgtatc attaatattg tcactatggt aaataacgag
     6121 tttatatttt tgtaaatcgc taaaagctaa tgatctaccg cgaatatctt cttcggcgtt
     6181 tatcttgcct ttaacatggg tacgacggct atatacatct actgggccag tataatcaga
     6241 cagcatattg gcatattttt catcgatcaa agcatctgga gcttgattag ggctatcaac
     6301 atcatcgtct acaatcaaaa ctccattacg gttagcgggt tcgatgtaag gatgaagata
     6361 gaaggtaaat actgcagggg tgtgatcata ttcgtcttgt aaatctacta ctcttacttc
     6421 aaatgtatgc ttacctggtc cagtaccaac tggagcaggt aattgaccac tggttaatac
     6481 tacagtctgt cctaaaggag aattggttgg aattcgtaac caccgttttc catcttgatc
     6541 tgtaactcca ttttgactga aaggaaaggg tggatagtta taaggttcac catcgaagcg
     6601 aatatcaaaa gcagttattt cgctaaaata gtttttcctg gttttactgt ctaaaactac
     6661 atctactttc ttaccgtaag gcatagcaat tagatttgtc gtatctccat aatctgctga
     6721 attatcacgc acatctccat attcacccca ccaaccccag cggacccaaa gttttatgtt
     6781 aggagaataa actgcagtat attttccttc aaaatctttg aataaaggtg tggcataatg
     6841 gattgaaccc tgagtgatag ttctcggtag tactgttaat tcaggtgttg tatcatcacc
     6901 ataatcttca aaatgatggt tagttaaagc ataagttttt gtcggataaa taatggattc
     6961 aggtctgaac ccgggacgaa cacgaaaatt aataactgta ttagtatcag gaatagaaac
     7021 aactcctgcg aggtctgtaa cgcgggcagt tactcttgtt ctttgttgta agggctgccc
     7081 tgtttgttca tcataatcat aagttaaata gggagttgta tgtttagtta aaagatattc
     7141 attaatctta tctgttctat tatcatcgct aatagtggaa taccaatctg attcagttcc
     7201 gggaataaca ttaccttgag aatcaacttt catcatttga aattcatatt gatagggaac
     7261 tggtgtaata aaaggatcgg tatctttcat tgcgaaggat aatttcaagc ccgaaccgac
     7321 ctcttggtta tttggatttc ctttagttgt agtaattgaa caggtaggcc ggtcggaatg
     7381 agtacaaaaa ttacgccagg caggttgagg agttatagct ccccgattat ctattgctac
     7441 aacttcaaac cggcttgatt tgtctatggg atttccataa gcatctgcag aagggaaatt
     7501 gattacagca tacttttggg aagtccaaat agtgcggcgt gctgaaggat catccaaagg
     7561 aatattgtga tctgcacctt gcatataatg aattacccat ccggttccaa aagctagaag
     7621 attgtcagga gtaagctctc cagttgtgtc aatataatca taccccggag taggaatagg
     7681 attgttattt tcatctaaca ctctgaaagc ataaccggtt attataccat caggatcagt
     7741 agcatgccag tagatacgct gttggaatgt gtaaacaagg ctagtatcat aaccagcggt
     7801 tacatatgtg ctatcccaac cctcaaaaga ggttattctt attgttggtg gttgattggc
     7861 aaaacggcta ccagattttc cacatcccga tagcaacaat attgcactta aggttatcat
     7921 aatgatagac aagagtttaa tactcttaac ttgcatcatt cctcccaaac tttttaattt
     7981 gtaggttcta atgaataccc ttgaaaatca gatcaagata ttcgtcaata cttttattta
     8041 tatttttttg ctcaaggcaa gctttaatga ggttgaaata gagaatagaa gtagttattc
     8101 ttccaattcc accagggaca ggagtaatcg ctgatgcctt atcaaagcat aagttataat
     8161 ttacatcacc aacataaatg ctctgcccat cgtttccttt ttcttcattt atgccaacat
     8221 ccaaaatgac agcattttct tttatcattt ccggagtaac aaaattcgct ttaccaatag
     8281 cggcaattag aatatcagct ttttgggtaa tttccgctat attatttgtt ttactatggc
     8341 aaactataac cgtggcgttg gcaaatgact ttttccaaag cagcatattt gctaaaggtt
     8401 taccaacaac attactgcga cccagaataa ccgcattttt gccttgagga ttaattccat
     8461 aatattgcat acaaaaaagt actgctgcgg gtgtacaagg aagaaatcca ttttcttcgc
     8521 ttagtatttt tcctaaatta agaggatgca agcagtccaa gtcctttccc ggatctatag
     8581 ccatattaat aatattatcc ttaatatgcg aagggagagg tttttgcagc attattccgt
     8641 ttatatcggg attttgatta gctccttcca taatagccaa taattcttgt tcagttatct
     8701 gttccgggag atctatcact tcgcaattac attgtaattt ttttccgcta ttgataatgt
     8761 tttgcacata gtaatttgag gcaggatcgc ttcccacctg aattaagagc attgttggtt
     8821 ttagttcgta ttgtgttatt aaagttttag ttgctttatt aaccaattga gcaacgggtt
     8881 ttccacttaa gatcttttcc atagtactta atcaatattc ctttttatcc gtgttatctt
     8941 ttcagcaaat cctgtttccg aatctaacac cagaaaaacg gcattcactt gtaaaccttc
     9001 atctgaggat tcaaaacgat tcggaacaga ggttctgaat ttctcaataa taatttcttt
     9061 tttaacgcca atcacgctat tgtgggaacc agtcatcccc acatctgtta tataagctgt
     9121 tcccaagggc aatatttctt catccgcagt ttgaatatga gtgtgggttc ctaaaactgc
     9181 actaattttt ccatctgcaa accaacccat agctcttttc tcacttgtag tttcagcgtg
     9241 aaaatctaaa attatgggat agggtagagg gatattgttt tgcttctcgt tatctataga
     9301 taaactgtac ttaataaacc tatccaacgc tgcaaaaggg ctatcacaag gtggcataag
     9361 taattgtccg cataggcaga taattcctaa gtggagatct cctttagata aaactaaata
     9421 agaattgcca ggtgcttctt cagagtaatt tagaggttta acaatccttt tttggctatg
     9481 gagatattct aaagattctg ttctatccca caaatgattg cctccagtaa ctgcatctac
     9541 tccagaattg aatagctgcg atagagtttt ttctgtaagg cctttaccat ctgcaagatt
     9601 ttctgcattg gccaaaacaa aatccggatt gaactcctct ctcaattgag gcaatgcttg
     9661 ctgaacagtt cgccgaccag cttttccata aacatcgcca aaaaaaagaa ctttcattat
     9721 tttgccatag ctatctggcg cgtttctctt atcacggtta cttttatttg acctggatat
     9781 tgaacttgct tttcaatgga ggcagcaatt tcagttgcta aaagagtagt ttgattttca
     9841 tctaccaaag caggctcaac aatgatccgg agttctcgtc cagcttgaat tgcataggat
     9901 ttactaacgc cttcaacgct attggctatt tcttcaagct tagataaacg ctgcatatag
     9961 gtttctaaca tttctcttct ggcaccaggt ctggctccgg aaatagcatc cgaggcttga
    10021 gttaaagctg cataaacaga tattgcttcc acttcttcat ggtgagcctc aatggcgttt
    10081 acgataatct ttccttctcc atttttacgg gctagattag ctccaattat agcatgagag
    10141 ccatcaaatt catgatctat tgcttttccg atatcatgta acagacctgc tcggcgagca
    10201 agttcttgat ctaatccaag ttcggcagct ataattccac aaatccaagc agattctatt
    10261 gaatgctgta aaacattttg gccataactt gttcgataat gtaggcgtcc cagaacctta
    10321 attaggttag gtgaaatatt atggatatta gtttccagaa ctgctttctc gcctattttt
    10381 actaaatttt cttccatttc tttggtagtt ttagctacta cttcttcaat gcgtccagga
    10441 tgaattcggc catcggcaat aagcttttct aaagataatc tggctatttc ccgacggaca
    10501 ggatcaaagc aactaagcac aacggcttca ggagtatcgt caataattaa atctacaccg
    10561 gaaacctttt caaaagtgcg aatatttctt ccttctctac caataatgcg tcccttcatt
    10621 tcatctgaag gtaaagatat aaccgagacg gttgtttcag aaacatgatc cacagcaata
    10681 cgctgaatag cggtagatag tatttcagca gctttttgat tggcatccag ttttatttgt
    10741 tcaagcgtta atcgtgcatc atttgcggca acttgtctgg cttgtgaaat taattcttct
    10801 cgtaaaagcg ctatagcttc atctcgagag agccctgcaa tttcaactaa tttatttttc
    10861 tgttctgtga tgatttgttc atattcagtt ttcttgttag caagctcttc ttctttttgt
    10921 tttagttttc tttcctgttc tgtgaggtta gtttcttttt tatcgagcga atccagacgc
    10981 ttatctaagc tacttaagcg ttcgttatat ttcttttctt gagtgcgcag ttctttctgg
    11041 cgttccttaa tctcttcatc gaggatgcgt ttttgtttaa accattcttc tcgggcttcc
    11101 aagagagcag cttttttagc attttccgct tcaattttag cgtttctttc cagttcttca
    11161 gctatttttg tagattgtga gataagtttg aaagtgctat tgcgttttat aatataaata
    11221 aaaagcgcaa tgacaaagcc gacagccact ccaattacga gtgctatgta aggatttatg
    11281 ttcatagtta tttccttatg taaatttatt taacttcggg ttttagccca aagcgaatta
    11341 taatattgcc cgcatgaacc gtgtacgctt gtcattcgaa agccaagctt taagtgggaa
    11401 tctaccttgt gagttttatg tatccggaac cagccggcgc gcatgccact catggctcga
    11461 aacccaaaat tataatagct tttatgcaac ccatatcacg aattctgcag gcactatcta
    11521 tatagttatt ttggaaagca tctgatttaa gcgttccagt tcagacatta aactttgatt
    11581 cttatcgcct aaagttaaaa cttcttcttg ttgttgaagg cagatcagaa gtagcagttt
    11641 agtgaaatcc agattatcgt atagcttaga taaatcctca atttgacggt caatctcttc
    11701 agctatatgt ttggttcgtt ccggattatc acttcttaaa cgaaatctgc gtccaaagat
    11761 ctctacttca acagatttca tatttatttc tcgttatcca gcggcggtaa aattccgtcg
    11821 atctttttta tagcaccttc ggcaacagtt tcaaaaccag caaggattct ctgcagttct
    11881 ttatatctat tttccagaat gttatattca tcctgcagtg ttttgtactt tttttcctgt
    11941 tcaccggttg ccacatctct attttgaagc tgagcaatga actgagaatt ttcttcggcg
    12001 agttgcttgt taatttcttc cagttcttcc actttttgag taactgcagt atattgatct
    12061 atcagtttct gtatcttgtc ttgaaggttt gtaacattac tttccatcat ctttacctca
    12121 tagttatttt ccaagtattc tcgagctttt ttatcactga atcaacaaga taatctactc
    12181 gttcatctgt caatgttttt tctttgtctc taattttcag ccgtaatgtt aagctgcgtt
    12241 tgccattggg aatttgttta ccgcgatatt catcaaaggc aattatatca gttatcatct
    12301 tatcatctac ttctgcaatt gtttttacaa ttttgctata gggtatttct tctgagatta
    12361 gaaaagaaat atctctaata actgatggca agcgcggtat ttcctgatat tggatttcaa
    12421 ggttatgagt agcgactatc agattctcca tagaaaattg cacaatccag atatcctgtt
    12481 ttagctcaat aagatcgata tcaaaattct ctgccacggg tgcagttaat tttccgtaat
    12541 aggcaagttc cttattatca aggtaataag cagcactttc agattgtgaa agatagggtt
    12601 tttttgcttc ttgtacagtc cagttttcca aatgcagcag agatagcaat tcttccacga
    12661 tgcctttcac ccaaaaaaca tttatggggc ttgctttatc ttgccagtgt tcatctttgt
    12721 ttagtcccgt taaaattgca gctaaataat agggttcgta agatatagtt tcttggcggt
    12781 gataagtttt tcccagttca aagagcttaa tattcttttc atcccgattc aggttatagg
    12841 ctaaattctg taaaagctga ggaattaaag aagtgcgcat agcactttga ttactgcttt
    12901 gcgggttttt cagaaaaatc atatccaatt cgtagtcacc ttcttggtaa ccaagcttac
    12961 gcattaattc cggttctgaa aaactgtaat tgagcgtttc ataaaaacca cgactaacca
    13021 gataatccac tgctactttc ttaatccgat aggaatggcg gtccataata tgctgtggtt
    13081 ttgtttttac aggagcttta tcataaccgt ccaaacgggc aatttcttca atgatatcaa
    13141 tctcgcgttc caaatccact cttttggggg gaataagata gtagtgtgct ttttcctgat
    13201 tttgcagggc ggtattattc tgttttaatg tttccgaatc taactctctg atttcggaaa
    13261 tattggttat ttttcctggt ttccaaatac catatcgcag aaatttaagt cccaagcgtt
    13321 gaaggtagtc ctttattttt tcttcttcca gttcgtagcc gattactttt gcataacggc
    13381 tgggtcggac acctaagatc aaggattttt caggaacagg ataattgtca taaactactt
    13441 cgcagagttc agcatctgcc aattcacaaa taaattggct ggctcttaaa gatgcatctt
    13501 gggctgaaca ttcacttaaa tgtctttcaa atcgatagga agaatctgaa ctaatcttca
    13561 atttataaga agttctgcga atcgaccttg gatcgaaagc tgcactttct aaaacaattc
    13621 ttttggtatt ttcgctgatg gcagaaatat ttccacccat aattcctgct attgcagagg
    13681 gtttaattcc atcagcgata accatttcct cgccctccag aatgtaattt ttcccatcta
    13741 gagtagttat tggttcatct ttatatgctt ggcgaataac gattgctgga attcccggtt
    13801 tatcaggaag ggaggcaagt ttttcataat caaaacaatg caagggatga ccatattcca
    13861 acattacata gttggtaata tcaaccagat tgttgattgg tcttaacccc gatttgatta
    13921 aagcactttt taaccacagc ggggattctt tcactttaac cttatcaaat aaacgcgcta
    13981 tatagcgagg gcatagttca ggtactttat taataagttc cagtggcata gtttctgctc
    14041 tgcattttgc aactggtata tcgatttccg gaagttttaa gggtctgcct aatttagctg
    14101 ataaatcacg cgcaatacct atataaccaa gaaggtctgg acgattgggt gtaatttcca
    14161 gttcaaaaac agtatctgga agctcatata gctcattaac acttctccca attggggtat
    14221 ctggttccag ctcaattatt ccactatgat tatctgaaat acctaattct ttttccgaac
    14281 agagcattcc gttagattca attcccttta ttttcgcctt ttttatagtt atatctgcta
    14341 aagtagatcc cggtaaagct attacagcca tcattcctgt tttacaatta ggtgctccac
    14401 atattacctg tagatagcct tctggagttt tttcaggata gggatagttg ccaatatcta
    14461 ctaaacagag ttttaaatgg tctgttttgg gaactggttc agcactaata acttttgcgc
    14521 taaaaacagt ttctggaagg gcaggtatat tttgaactgc ttccacttca ataccggaga
    14581 aggttaaaag ctcttccagc tccttagtat tttcttttat ctcaagatac tttgctagcc
    14641 aggaaataga gatcttcatc ttagctctcc tttgaactga cgcagcattc ttaagtcgtt
    14701 ttcatacaat atacgcagat caggaatatt atatttcagc attgcgattc tctctattcc
    14761 taaaccaaat gcatatccag tataaatttc agaatctatt cccagaatat caaaaacatt
    14821 aggatctacc attcctgctc cacccatttc tatccatcca gagtatttac aaactctaca
    14881 tccagttcct ccacaaacta cacaggaaat atccatttca gcgctgggct ctgtaaatgg
    14941 gaaaaagtga ggtctgatcc tactggaaac atttttacca aacataatgg aagcaaattg
    15001 agatagcata tctttcatat ctgataaaga aataccttta tctacaacga gtgcttctac
    15061 ttgatgaaaa acaggagaat gagaaggatc tggtttatca tttctataac agcaacctgg
    15121 agatattatt tttatgggag gaggatattt ttccatcact cgtacctgca ccgttgaagt
    15181 atgagtcctt aaaagtcctc caccttccag ataaaaagta tcagcaagat tacgggaagg
    15241 atgatcagga ggagtattca acgcatcaaa attgtgaaaa tcatcatcaa tatctggccc
    15301 ttcagcaatt tcaaaaccca agcccaaaaa aacctcatca atttcacggc ggatgatagt
    15361 taaaggatgt aaaccaccac gcgagctaaa aataccaggc atcgtaaggt ctattgctgt
    15421 tctgttttca ctctttttcc aggcatcttc tttgatcgct ttattttgct tttccagaag
    15481 atcttcaata ttcttgcggg ttgcatttat caaatttccg aaagcaggac gttcttcgct
    15541 cggtatatct ctcatcttag catatagaga attaagaaca ctcttcttgc ccaggtattt
    15601 agcttttata ttcaatagat cgttataact ggcacaagcc aaaatatcgc tctctgcctg
    15661 tttaacgagt tctttcaatt cttcttccaa cttatcctca tttatttatc tgcatacaga
    15721 ttacgcagat tatacagatt taatgaaaag gtaagagttt tattaaataa aaaggactaa
    15781 tcaggatttt atatatatag ctccagaaga tatatggaat atttctcagc gtatctatcc
    15841 tgtttatcat ctataatcct tttaatctat ttttgtctta aaaacctcat tgcttttttt
    15901 atcagtaatc tgcataacct gcgtgctaca atttcttatc ccttagcgat ttctacaagt
    15961 ttggcaaaag cgggagcatc atgccaggcc aagtgtgcca atgtttttct gttaatttga
    16021 atatttgctt tatgtaaacc attgataaat tggctgtagc tcatatcatt tattctgcag
    16081 gcagcattga ttctggttat ccataagctt ctatactggc gttttttcag tttacgatga
    16141 gcaaaagaaa agaccattgc tctttcaaca gtttgtctgg ttgcgcgata ggttttactt
    16201 ctgtttccaa aataacctcg agcattcagc atatattttt ttcttctacg atgagcagcg
    16261 acattattag ttattcttgg catttttatc ctcccttatc tataatccca gcatccggtt
    16321 gattttcttt tcatcacatt ttttcacgat ggcacttgtg cgtagatttc gttttagttt
    16381 tggtgatttt ttggttttaa tatgtgcgct ttttgcatga tgacgcacta tttttcctgt
    16441 gccggtaacc ttaaatcttt tagctgccga acggttagtt ttgagcttag gcattttaga
    16501 tctcctttat attaaaattt tccccgctcc ggaatgccta ttatcaaata ttatccggcg
    16561 aggaacaatg gaaataataa ttactttatg cctgaggatc tgtcttttct gaatcagacg
    16621 caggcatttc ttcttttttt gcttctttgg ctaaaatgcg atcaatgtct tttttgggag
    16681 aaaccactat agatagcata tttctctcgg ataccggctc actatcaatt tcaatgagat
    16741 gggcgagatc ttcttttagc ttatccaaaa ccttgtaacc aagttcttta tgagccattt
    16801 gccgtccccg aaaacggacc gtaaatttca ccttgttatg ctgctttaga aatttgatgg
    16861 cgttgttttt cttgaagtta taatcatgtt cttctgtatt aggaccaaac ttgatttctt
    16921 tcacttccac ttcatgctgt ctttttctgg cttcccttgc tttcttttct ttttcaaagt
    16981 aatacttgct gaaatccaga atccgacata caggcggttc cgcattagga gaaatttcca
    17041 ctaaatcaag ctccatatca tttgctttgc gaagagcttc ttttaaagaa actattccta
    17101 tctgtttacc atcgggaccg atcaaacgga ctttatcagc cgtgatctga ttattaattc
    17161 tttccttggg caccacttca tttgtgcgtg cctttccttt cttaatccgg attataagaa
    17221 cctccttctt accggtaata aaataggcga ttttctttat agagaaaacc gcctgcttca
    17281 cagtaaatac tttttgtatt atttcttgtt atcgcaaaaa ccttatcagc aatctaaaag
    17341 ccataaggca gaagcaagca gcttcatttt tatagtcatt aattttagca tctgcattac
    17401 tgtcaatcat ttttttcatt aacattgggt ggtaaaggtg atgagtatcc ccctaccgaa
    17461 gattataaat gccactcatt tttttatttc ctaccgctta ctgtcatccc tcttattttg
    17521 gttcattaat atatgcagat gaatagtaca aaatataagg gaagatctta cttctaccac
    17581 ccattcttac aaaaaacatc tccttaacca cttctcatca acccatcctt aaagaggaga
    17641 gaaggatggg tggaagagga gttgaggagg agagagtcgt tctgaattaa gagcactaaa
    17701 attgggggaa aagaaatgaa caaaaagtct actgaagaaa atgcattata agaatagtga
    17761 tgccttagaa cataaagttg agcagaaaat aaaaaaaggg atagttgaaa attagggaga
    17821 taaatcgtct ataaaaacta atggcaacca gttttttgca ttacttctta cctttgtcgt
    17881 acggaaaata aagtgtgaga agtatttgat gaggacatca accttctggc tttttttatc
    17941 gctgggttaa aaaccccgtc gttattatat gtcattccta actgaactca aattttgaca
    18001 ggatttttag tatagataaa atcaaaaatc agtctactat tttaaggcct gaaagtaagg
    18061 actaattcta tacttctgaa ataacaaaat gagagtagca ttgtgggatg gccagaacga
    18121 aaataatgca taaataagcc agaaacggtt acttattatt gacattttta tggcttgtaa
    18181 tattatgaaa ttgtgtatta tagtttttaa atcggattac agaaagagag aagattatgc
    18241 aaatatcgga tgggatatct tttccagata ttaaaaagca ggaacctatt attatttctg
    18301 cttcgcgggc tacagatata cccgcttttt atactgactg gttaatttcg cggatagaag
    18361 caggatatat aaaatggaaa aatcctttta atggtcaaat taaagatgtc tcttttgcta
    18421 aaactcgact ttttgtgttc tggagcaaaa atccttctcc tatgctgaga aagctgtctt
    18481 actttgatga gaaggg
//
LOCUS       DAOSTH010000026        17932 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3267_length_17932_cov_12.167981, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000026 DAOSTH010000000
VERSION     DAOSTH010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 17932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17932
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3267_length_17932_cov_12.167981"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLB65_04735"
     CDS             <1..365
                     /locus_tag="PLB65_04735"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ77869.1"
                     /translation="IMPIEGHIISEYGLRNGRPHKGIDIGAKTGTPVYAVLDGTVVYS
                     GVQGSYGNVVVIEHPDFVMTVYAHNERNLVSVNDVVKQGQQIATVGSTGNAQGSHLHF
                     EYRLKGKAINPRKVLPLK"
     gene            378..1256
                     /locus_tag="PLB65_04740"
     CDS             378..1256
                     /locus_tag="PLB65_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HOQ77870.1"
                     /translation="MSRKDSVFADKALQNYLNEISKFKTLSREEERELAIRAKNGDVE
                     AMNRLIQANLKFVVKIAARYQNRGLSLSELISEGNIGLIKAIEKFDPDKDVKLISYAI
                     WWIKQRITLAVSEKSSLIRVPMGKTSAAHRVKATQDRYFTETGEMASTDELSEKLNIA
                     EKTIDQLQNQLPETSSFDDIITTLDSQDFTTKDLIEDKETVDPQKLYYKERMQERIQR
                     AINKLDQREADVIRSYFGLNESHESRNFAQIAESMGLSRERVRQIQKEALKKIMREIK
                     PEEDEFVDRFLEKYHY"
     gene            1320..2231
                     /locus_tag="PLB65_04745"
     CDS             1320..2231
                     /locus_tag="PLB65_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HOQ77871.1"
                     /translation="MFHNFDEMLSYVKQNCKGIAIIAGAQIESALEAAIMAKKGNLCN
                     SILVGDQAEINRLLQEIAPELMDSFEIIDTGSNLIKAAETAVALAREGRGDLILKGKM
                     ESSVILKAALDKDKGLKVSDVISDVFVYEHPEGLKIQTDGGINILPELKEKIAMVKNA
                     VQVAHSLGNPNPNVAMVCAIETVNPKMPATIDAALIAKMNERGQITGCKIEGPLAFDN
                     AVDKEAARIKGITSEVAGAADILIFPNIETGNVHGKMLTYYCHYRIAHVTIGTKVPIL
                     IPSRADSGETKMLCMALALATMAKSKK"
     gene            2228..2689
                     /locus_tag="PLB65_04750"
     CDS             2228..2689
                     /locus_tag="PLB65_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOQ77872.1"
                     /translation="MKLTLLQTGKTKDKWLLEGIAEYQKRISAYSKFQIIDLPDESIK
                     NSGNANIVKAKEANTILKAIKPDDYVILLDEKGEPISSLAFADFLVNISDKNVVFVIG
                     GVFGVDENIRKRANKILSLSHFTFTHRLARLVLCEQIYRALMIINNRNYHN"
     gene            2705..2854
                     /locus_tag="PLB65_04755"
     CDS             2705..2854
                     /locus_tag="PLB65_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77873.1"
                     /translation="MLTIIATVLDNVKLSLGLMVQGMLGIFIFMAIFYLLIYALERFI
                     KPKEK"
     gene            2854..6687
                     /gene="porU"
                     /locus_tag="PLB65_04760"
     CDS             2854..6687
                     /gene="porU"
                     /locus_tag="PLB65_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HOQ77874.1"
                     /translation="MRRLLLFTLLIFTITFLNAKLTIIEKSETEFLLEFKLEPWELVA
                     EGNYSRIVAENMDYNNNTGAPLIPYDEFKVGIPPSGNINYSLLNSNTTSITLPSKLLP
                     VPEIKKVDGISSYEYKISPEEYQLSRTEFITELPVSNFRGYNFIPFIIYPFAYDGNKT
                     LTLTTQASIIINISGQTNFRSLEPSDKAADVFLAQLINEQQTRNWRNNPRPAIHYAPF
                     ANSDWWVRIETNREGMYRINRSDLSGLPLNDIDPTTIRIFTTTGKVLGNNIDDIGAEF
                     KEIPIIISGEADHKFDPEDYILFYGSSRDGYEMNANVQDNQYYNPYSQNQVFWLSFGG
                     SFNGNPIRIQTITPPSSYDVSVTSTPAVKHIENEIYRREETGFDWYSQTLFGNSTAEY
                     NFTTDLTDIDLSGEQIVQLRLRQEDTGEGTNHRIQVYVNGWPIIYSSDPDVYDFTWSG
                     TWPFTIKRNTTHLRNGSNTITIKVSRTTTDNLYLDYIHLAYQQNLNKSNIQKQFSHTT
                     SLYPIPCKFNLSGDLSNILVFRSNGIYSVDKIPLTDSYFISEGTSSTSYFILKPNETY
                     NPALIRVMHPTDLTANISQVDNIIVSPSEFISHAQVLADKYWDYYQVRSKVILVDDIY
                     NQFNGGHIDPAAIRLALKYFYSNFTAPRISSLTLLGLGTIDWRNFSGSAATKNKMIVW
                     QNGFLTSDDYFGMINTDYYPELAIGRYPVKNTAEMNIMLQNFSNYTSNPSHGWWRNSM
                     IFLADDLTNGPSNYEYIHTQDIEDIGSVLNPSILADKIFAMEYEYDEFQNKPRARDDM
                     FKAINEGRLVFLYAGHGSFDKLGAEDYLNGATDMGRFANPDKLTLFIAASCDVSQFDY
                     WGFESLGQKVVLMNNLGAIASYAATRKSYAENNRPMMKYLMESLADNRNPLGYSIMDA
                     KIRYTGSNSNDAVYVLLGDPLIRVIPPERDSTMQVNLLEDNVVLHSRETAIINGSFST
                     PGLSGEAEVRIFDPIRKYSLGPNTNVSKKGNQLFRGRSEVTASQYNSAFIVPDDIISG
                     NKGLCVSYIWDEATKKDYTNYYFPLGFSDQAVDVDNPDTPQINIYLGHKDFRPGDTVG
                     TNTTLYADFYDSNGINITGSSGHNILLVIDNSLQPLAITDYFNYDRNSCTNGNITYPL
                     SNLSEGIHTIQVIAFDNFNMPAVATTHFVAKKQTELDIEQLLIYPNPLSKDTYITFIL
                     TSDAQVNIGIYTLRGKRIHNLKVYGKQGFNKIYFSGKDDQGDTLSNNTYFLKVQATSA
                     DGKKVEKTEKMVIYK"
     gene            6699..8108
                     /gene="cysS"
                     /locus_tag="PLB65_04765"
     CDS             6699..8108
                     /gene="cysS"
                     /locus_tag="PLB65_04765"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOQ77875.1"
                     /translation="MLLYNTQTRKKEKFIPITPGKVGIYACGPTVYNYFHIGNARAFI
                     FFDVVRRYFEYQGYEVTYVQNITDIDDKIIAQANEENTSFHMVADKYTRAFLEDIAAL
                     GINKPTYQPKATEVMNDIIAFIAALEQKGFAYSVEGDVYFATEKLPSYGCLSGKKMEE
                     QLAGARVVENPNKHHPADFTLWKKGKPGEPVWDSPWGKGRPGWHTECVVMSKKYLGDT
                     FDIHCGGIDLIFPHHENELAQAMAFSDKPLSNYWMHNGFLNVDGEKMSKSLKNFFTAR
                     DILAHYDAEAIRFFFLSKHYRSPIDFSRELIEEAQRAVANLYSSLSEINYLDNVQNSP
                     QTMCGTAVELQNLHKEYITAMDDDFNTAKAISILFELNRKVKNTSSAFADRLAAAQKL
                     VELGSILGFFQNLEEQLSTFMPDLSKDLIELILSYRQESRRNKQWTVSDRIRDDLARL
                     GIEIKDTPEGVKWNLKEKK"
     gene            8105..8920
                     /locus_tag="PLB65_04770"
     CDS             8105..8920
                     /locus_tag="PLB65_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77876.1"
                     /translation="MKNKTLYLALVLLIVVAIIIVVLLISRKAPLNGPESIAYDDVSK
                     QFLVSNTLSKNIVSMSLNGQFTPFLKKGLQEPRGIIIYSHKLYVADKASIKVIDLDKA
                     EIIDTINIPGALMLNDLAFTKQGVIYTTDTAGNCVYVIDTLSNKIQKIANPLLQKPNG
                     IIYDMPRDQMFVVCFREKSPILAISTLDNSISIFMDSIYSDLDGIAIDDLGRIYISSW
                     GQNMIFEIPQEQNRFLATFTDIEDAADILYYLPNNELIVPLFNKNKIIRLKLE"
     gene            8913..9548
                     /locus_tag="PLB65_04775"
     CDS             8913..9548
                     /locus_tag="PLB65_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOQ77877.1"
                     /translation="MNKKDSTYLIILPDCVIKHFNDPKHFQAELYAYQAGLRMLPDLL
                     DFQVPQWIKIEKVKGIPYLDRLDYFYPELLAETYANFHLATLKNDKCLCHVDNSPKNI
                     LFCQGKYYFIDFADSRFEYPERDITHLLLFWAADFPADYFKYALETFLNGYLSFVTLN
                     SERWNSYLKSNIILFDQRRKLHSKKTGKESPSIQNLNRELLAQMKLCYMKE"
     gene            complement(9727..10524)
                     /locus_tag="PLB65_04780"
     CDS             complement(9727..10524)
                     /locus_tag="PLB65_04780"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC/CoxI family protein"
                     /protein_id="HOQ77878.1"
                     /translation="MDNLTFYEKISIFLKEHKTVWQASIIESDGSTPAKCGMKLAVTL
                     NEEPFGNLGGGDLEHTVIEMIKKEKPRTAQIFSFNLSTDGAKTGIATSMLCGGQVKIY
                     IEPLFLSDKLYIIGAGHCGKALGKLARLCGFYVYLIDNRKEIIDNLLEDCYHTAILHN
                     YTDLSEIIDFDSDSWIVIMTQGHLHDKEVLEQCLRTNFRYLGMMGSKKKVLDTFAKLK
                     EEGFTEKELFRCHSPIGLPIGSNTPEEIAVSIMAELISIRNSKNNER"
     gene            complement(10517..11326)
                     /locus_tag="PLB65_04785"
     CDS             complement(10517..11326)
                     /locus_tag="PLB65_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOQ77879.1"
                     /translation="MKFSACYSATKFKAFTYEEQIKALHKLILCLEQNLSNPEKRLSI
                     IDHLLKLSQLVDEPLPQKMHFFLKELNPRLSPHQLMNKLYFYLEGSLRKDGSMSLRIG
                     DGKISADPFLRKKAKQITVICDNLRSVFNVGSLFRTSECLGIGKILLCGITPTPEHSN
                     MPKTAMGTEKLVEWQYLQTSIDAITICRESGMYIYALETVEEAGSIFQTIYKLPLAIV
                     IGNEALGIEPSVLELCDECIALPQLGWKNSLNVGVATGIALYQIIFGGPNG"
     gene            complement(11323..11913)
                     /locus_tag="PLB65_04790"
     CDS             complement(11323..11913)
                     /locus_tag="PLB65_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOQ77880.1"
                     /translation="MTAENIKSAIFLDRDGVISPDDFGYISEPEQYHLYPYTIEALRI
                     FKELDFLLFVVTNQSGIARGYFSIEDLERVLAKMRSLISAGGIELDGVYYSPYYKDGI
                     IPPYNIDHEDRKPGIGMFKKAYSDFHFDIEQSWMIGDRHSDIAFGKNAGLKNILLLTG
                     NGHKDFIEIIKTNELKPNFVCENLLTAANLIRDFKL"
     gene            complement(11894..14317)
                     /locus_tag="PLB65_04795"
     CDS             complement(11894..14317)
                     /locus_tag="PLB65_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HOQ77881.1"
                     /translation="MKNTLIRLFLVSLLMLIMLPICAQSEAISPAMPDTLFPEIPDSL
                     DINIVKTDSLFYKADSIHFEYTTEIIKLYGKPQINYQDTEIIADSITIDIKNECAYSY
                     GPTRMKDGDQLLLGTNIRFDVNTQTGILDDGKSLIEGGYYSGQELRKTDKDTYDIDGG
                     TFTNCDLEEPSFWFWAKQLRVYKGDKIVGKPVIGYVNHMPVFFFPFITIPIRQGRYPG
                     FLIPSPDYNNVDGFVIRNLAVYYPYKDYADFVFGFDIMEKTGWKGHFDTSYLKRYSFS
                     GNLNASYQHNLKDDVTYDDWSLQGRHHQDLPEKSSLDANINFVSNKRIWESSSNVDQS
                     LAQRLTSSVSYSKPIGSSYFNAGSNFTQDLINDTATLSLPSASFYLASRPVSELLNVS
                     SDSWLSNFNYRYNAYLEHTGYLKEKNYSISDLFWDNTVDPSDTTGLTMLNEHHFGIKH
                     NAGISRNSKLFGWLTLGESFDYSEAWMDRDKQNDKYARGNAWTASVNARFNLYGLRNF
                     SNFPVTSVRHIITPNIGYSYQPDTRKNSDLYNFGSIAVSSVKESSNLNFSVDNNWQLK
                     YGTKGKETKLNDLLYWRIGTSANLKKKDQPFSNISHNLTFKPGTYKIGTLSINDGKYQ
                     VKDIKVGYSSQFSATQNPYQIHWNDMNLRNQYFSQGISISGSAPYTKYFVAPKNRNFE
                     VFESYDSLNAYPKSFQEDTGSNDWSFSISQNIYADKDIFHSESSNLRMNLTCKITENW
                     SLAYTNYYNLKTSNMLAQTLSISRDLHCWKMDINITLRNDYWDYRITLFNTLLPDALR
                     FQTHDSRKY"
     gene            14432..15073
                     /locus_tag="PLB65_04800"
     CDS             14432..15073
                     /locus_tag="PLB65_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77882.1"
                     /translation="MPSCKATLYSLISILILLSLTACKIDMKQEIWLKKDNSGKASVI
                     VHMQIPFGLQNETLDLQELNSFNALSSLAEVARKTPGVSVTRLAQEETHNKKEMNLSY
                     YLDFTFKDVEGLSTVLSAEGRNAIFLTKSGKHKVLCIKAEELALIEDDDSDEFDFLSF
                     IEINLRNKVNLPARVKKIDNTGYGKYKGKTATWDIELDDDWLSCTSHLLYVHF"
     gene            15083..15853
                     /locus_tag="PLB65_04805"
     CDS             15083..15853
                     /locus_tag="PLB65_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOQ77883.1"
                     /translation="MKLTLWSWNVNGLRAALNKGFIKIIQNEQPDILGIQETKLQEHQ
                     IPEELNTLANYLIYWSHSIRKGYSGTGLFTKILPISFSTSFGNPEFDSEGRINIAEYD
                     KFIYFNIYFPNGQKDDERLNYKLRFYDRCLEMMEEKRTTGKIILVGGDFNTAHKPIDL
                     ANPKENEKTSGFLPIERAWLDKIETMGWVDTFRHFDKSPEQYSWWSYRTKARPRNVGW
                     RLDYFWVNQEGLKHIINAGIRQDIEGSDHCPVFVELEI"
     gene            complement(15878..16660)
                     /locus_tag="PLB65_04810"
     CDS             complement(15878..16660)
                     /locus_tag="PLB65_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HOQ77884.1"
                     /translation="MRKFLLLLILLLLSCTQSSKTISISQFEQRIWELTNQERIAHNL
                     SPLLYDEGLADLARMHSSNMFKYDFFAHKDHLGYLVDDRKRRYYPQLIVASIGENLAK
                     IVSGAKIFTPEEIVEGWMNSTEHRENILNGSFTHLGVGVFTQGSVLYATQNFGTPIAK
                     LLSQIPEQLEVKNKYQLQFEFMSLEPVNELEAILNFPESNFKYNIDDQYYTLGLKPIP
                     IHWITDKQFEVIIDFPAGKGDYNLCFGFNRSYFADGIKLKVK"
     gene            complement(16676..17125)
                     /locus_tag="PLB65_04815"
     CDS             complement(16676..17125)
                     /locus_tag="PLB65_04815"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HOQ77885.1"
                     /translation="MKRRILALITLTISGCLLAQPVNKDPTKAVIYSLFPGGGQIYNE
                     AYIKAGAVIGFQSYLLATAIHNNDKVNDYKDKISITTDPILIESYKSKQKNYKEKRTR
                     DIWLMGLTMAFSTLDAYIDAHLSNFNSDKEKVHLRFEKDILLLEYEF"
     gene            complement(17272..17359)
                     /locus_tag="PLB65_04820"
     tRNA            complement(17272..17359)
                     /locus_tag="PLB65_04820"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17323..17325),aa:Leu,seq:aag)
     gene            complement(17364..17702)
                     /gene="secG"
                     /locus_tag="PLB65_04825"
     CDS             complement(17364..17702)
                     /gene="secG"
                     /locus_tag="PLB65_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOQ77886.1"
                     /translation="MVLYSIALIIHVIICIALVLVILAQTSKGGLDSNLGGAAMNVFG
                     GSGASKFLKKWTQILAVVFAASCVLLAFLVRDINSSALTGIQERQKKIKDTEQQAPAA
                     NPIPKQVPTK"
BASE COUNT         5938 a         3127 c         3221 g         5646 t
ORIGIN      
        1 taattatgcc gattgaggga catattattt ccgaatatgg tttgcgaaat ggacgccccc
       61 ataaaggtat agacataggt gcaaaaacag gtactcctgt ttatgcagtt ttagacggaa
      121 cagttgtcta ttcaggagtt cagggttctt atgggaatgt agtagtaatt gaacatcccg
      181 attttgtaat gacggtttat gctcataacg aaagaaattt agtaagcgta aatgatgtag
      241 tgaaacaggg gcagcaaatt gctacggttg gttcaaccgg taatgctcaa ggcagtcatt
      301 tacattttga ataccgtttg aaaggaaagg ccatcaatcc ccgtaaggtt ttgcctttaa
      361 aataaggaat aagaagagtg agccgtaaag atagtgtttt tgcagataaa gccctacaga
      421 attatctgaa cgaaatatcc aagtttaaaa cactatcgcg cgaggaagaa cgagaacttg
      481 ctataagagc caaaaatgga gatgtggaag caatgaatcg tctaattcag gcgaatctga
      541 agtttgtggt aaaaattgca gctcgttatc aaaatcgggg attatccctt tcggaactta
      601 tcagtgaagg caatattgga ctgattaaag ctattgaaaa gttcgatcca gataaggatg
      661 taaagcttat ctcttatgct atctggtgga ttaaacaaag aattaccttg gctgtaagtg
      721 aaaaaagttc tctgataaga gttcctatgg gaaaaacaag tgcagctcac agagtaaaag
      781 ctactcaaga tagatatttc actgaaaccg gagaaatggc ttccacggat gaacttagcg
      841 aaaaacttaa tatcgctgaa aaaactatcg atcaattgca aaaccaattg ccagaaacaa
      901 gttcttttga cgatattatc actacactcg atagtcagga ttttactacc aaagacctca
      961 ttgaagataa agaaacagtt gatccgcaaa aattatatta taaggaacga atgcaagaaa
     1021 gaattcagag agctataaat aaacttgatc agcgtgaagc tgatgtaatt cgtagttatt
     1081 ttggtttgaa tgaatctcac gaatcgcgaa attttgccca aatagccgaa tcaatgggtt
     1141 tatcaagaga aagagtaagg caaattcaaa aggaagcatt aaagaaaata atgcgtgaga
     1201 ttaaacctga ggaagatgaa tttgtagata gatttctgga aaaatatcac tattgaacaa
     1261 taatgatccg tgagaaatta aatttctatt ttatatacat tataaataag gagtgaaata
     1321 tgttccataa tttcgatgaa atgctgagct atgtaaaaca aaactgcaaa ggaatagcta
     1381 tcattgctgg agcacaaatt gaatcagctc ttgaagctgc aataatggca aaaaaaggta
     1441 atttgtgcaa tagtattctg gttggtgatc aagctgaaat aaaccgtcta ttgcaagaaa
     1501 tagctccaga gcttatggat agctttgaga ttattgatac cggcagtaat ttgataaaag
     1561 ctgcagaaac agctgttgcc ttagcccgag aaggaagagg agaccttatt ttgaagggca
     1621 aaatggaatc gtctgtaatt ttgaaagctg ccttagataa agataaagga ttaaaagtaa
     1681 gcgatgtgat ttctgatgtt tttgtctatg agcatccaga aggattaaaa atccaaactg
     1741 atggcggaat caatattctt cctgaactga aagaaaaaat agcaatggtt aaaaatgcgg
     1801 tccaagttgc tcattcctta ggaaatccta atccgaatgt ggctatggtg tgtgccatag
     1861 aaacagttaa tcctaaaatg cctgctacta ttgatgcagc attgattgct aaaatgaatg
     1921 aacgcggtca gattaccggg tgtaaaatcg aaggcccttt ggcttttgat aatgctgtag
     1981 ataaggaagc agctcgcata aaaggtatca cttctgaagt tgcaggtgca gcagatattt
     2041 taatattccc caatatcgaa acaggcaatg ttcatggtaa aatgcttact tactattgtc
     2101 attacagaat agctcatgtt actataggta ccaaagttcc tatattgatt ccttcacgcg
     2161 ctgacagtgg agaaactaaa atgttgtgta tggctcttgc cttagctaca atggcaaaat
     2221 caaaaaagtg aaactaactc ttttacaaac aggcaaaact aaggataaat ggttactgga
     2281 aggaatagct gaataccaaa agaggatctc tgcttattcc aaatttcaaa taattgattt
     2341 acctgatgaa agtattaaaa attccggtaa tgctaatatt gttaaggcaa aagaagctaa
     2401 tactatctta aaagcaatca aacctgatga ttatgttatt cttttagacg aaaaaggaga
     2461 gccaatcagc tctcttgctt ttgcagattt tttagttaat atatctgata agaatgtagt
     2521 ttttgtgata ggtggagttt ttggtgtaga tgaaaatatc cggaaaagag caaataagat
     2581 tttgtcctta tcacatttta cttttacgca ccgcctagca cgattggttt tatgtgaaca
     2641 gatttatcgg gctttgatga taatcaataa tcgtaattat cataattaat tagaagggga
     2701 aacaatgcta actataatcg ctactgttct ggataatgta aaattatcct tgggattaat
     2761 ggtccaagga atgcttggaa tattcatctt tatggccata ttttatttgc ttatttatgc
     2821 tttagagcgt tttattaagc ccaaggagaa ataatgcggc gattattatt atttaccttg
     2881 cttattttta ctattacttt tttgaatgct aagttaacaa tcatcgaaaa aagtgagaca
     2941 gaatttctac tggaatttaa gcttgagccc tgggagttgg ttgccgaggg taattatagc
     3001 cgtattgtgg ctgaaaatat ggattacaat aataacaccg gcgcaccgct aatcccttat
     3061 gatgaattta aagtgggaat tcctccttct ggaaacataa attactcatt acttaacagc
     3121 aatacgacct ctatcacttt accttcaaaa ctcttgcctg ttccggaaat aaaaaaagta
     3181 gacggaataa gcagctacga atataaaatc tcaccagaag aatatcagct gagcagaact
     3241 gaatttataa ccgagcttcc tgtttccaac ttcagaggat acaattttat tccgtttatt
     3301 atctatccct tcgcttacga tgggaataaa acccttacat taacaactca ggcaagcatc
     3361 ataataaata tttcaggaca aactaacttc cgcagtttag agccaagcga taaagctgca
     3421 gatgtttttt tggcacaatt aataaatgaa caacaaacaa gaaattggcg taataatcct
     3481 cgtcctgcta tacattatgc tccgtttgca aatagtgact ggtgggttag aattgaaact
     3541 aatcgcgagg gtatgtatag aattaatcgt tcagacctta gtggcttacc tctaaatgat
     3601 atagatccga caactattcg cattttcact acaactggaa aggttttggg gaataatata
     3661 gatgatattg gagctgaatt taaagaaatc cccataatta tttctggaga agcggatcat
     3721 aaatttgatc cggaagatta tatcctattt tatggtagct ctcgcgatgg atatgaaatg
     3781 aatgctaatg tgcaggataa ccaatactat aatccctatt cccaaaatca ggttttttgg
     3841 ttaagttttg gaggcagttt caacggtaac cctataagaa ttcaaacaat aactcctcca
     3901 agcagttatg atgtttctgt tacttctact cctgcagtaa aacatataga aaatgaaatc
     3961 taccggagag aagaaactgg ttttgattgg tatagtcaaa ccctgtttgg caatagtact
     4021 gcagaatata actttactac agatcttacg gatattgatc tttccggtga acaaatcgtt
     4081 cagctccgtt tgcgtcagga agatacagga gaaggaacta atcacaggat tcaagtttat
     4141 gtaaatggtt ggccaattat atacagtagt gatccagatg tatatgattt tacctggagt
     4201 ggaacttggc cttttacaat caaacgcaat actactcatc taagaaatgg aagcaataca
     4261 attactataa aagtaagtcg cacaactaca gataatctct atttggatta tatccactta
     4321 gcttatcagc aaaacctgaa taagagcaat attcaaaagc aattttccca tacaacttcc
     4381 ctatacccta ttccctgtaa atttaattta agtggagacc ttagtaacat tcttgtcttc
     4441 cgcagtaatg gaatctacag tgtggataaa attccactaa ctgatagcta ttttatttct
     4501 gaaggtacaa gctctacaag ctattttata ctgaaaccaa atgaaacata caatcctgca
     4561 ttaataaggg ttatgcaccc aacagattta actgctaata tcagccaagt agataatatc
     4621 attgtttcac cttcagaatt tatttctcat gcacaagtgc ttgccgataa atattgggat
     4681 tattatcaag tacgcagcaa agtcatctta gtagatgata tctataatca gttcaatgga
     4741 gggcacatag acccggcagc aatccgtttg gcattaaaat atttctattc caattttact
     4801 gcacccagaa tttcctctct tactcttttg ggtttaggga caattgactg gcgtaatttt
     4861 tctgggagcg cagcaaccaa aaataaaatg attgtctggc aaaatggttt tcttacttcc
     4921 gatgactatt ttgggatgat aaacacagat tactacccgg aattagccat tggcaggtat
     4981 ccggtaaaaa ataccgctga aatgaatatt atgctgcaaa atttcagtaa ttatacgagt
     5041 aatccatctc acggctggtg gcgtaattct atgatatttt tagcagatga tcttactaat
     5101 ggtccttcta actatgaata tattcacact caagacattg aagatatcgg tagtgttcta
     5161 aatcccagca tattagctga taaaatattc gctatggaat atgaatatga tgaattccaa
     5221 aataaacctc gtgccaggga tgatatgttt aaagctataa acgaaggacg acttgttttt
     5281 ctttatgctg gtcatggatc ttttgataag ctgggggcag aagattattt aaacggagct
     5341 acagatatgg gtagatttgc taatccggat aaactgactc tatttattgc tgcttcctgt
     5401 gatgtatctc aatttgatta ttggggtttt gagagtttag gacaaaaagt agtactgatg
     5461 aataatttgg gagctattgc ctcttatgcc gctacccgta aaagttatgc agagaataat
     5521 agaccgatga tgaaatattt aatggaaagt ttggctgata atcgcaatcc gttgggttat
     5581 agtattatgg atgccaaaat tcgttatact ggaagtaatt ctaatgatgc tgtttatgtt
     5641 cttttaggag atcctcttat cagggttatc cctccagaac gagatagcac tatgcaggta
     5701 aatttattag aagataatgt agttctacat tccagggaaa cggctatcat taatggttct
     5761 tttagcacac caggcctttc gggagaggca gaagtaagaa tttttgatcc aataaggaaa
     5821 tattcacttg gtccgaatac aaatgtatcg aaaaagggaa atcagctctt tcgaggtaga
     5881 agtgaagtta ctgcttccca gtataattct gcttttattg taccggatga tattatcagt
     5941 gggaataaag gactttgtgt ttcctatata tgggatgaag ccacaaaaaa ggattataca
     6001 aattattatt tccctttggg atttagtgat caagcagtgg atgttgataa tcctgatact
     6061 cctcaaatta atatttatct tggtcataaa gattttcggc ctggtgatac agtcgggacg
     6121 aatactacct tatacgccga tttctatgat tccaatggta tcaacataac gggatcttcg
     6181 gggcataata tccttttagt aattgataat tcattgcagc cattagcaat tacggattat
     6241 tttaactatg atagaaattc ttgtactaac ggcaatataa cctatccact ttctaatttg
     6301 agcgaaggga tacatacgat tcaagtaatt gcttttgata attttaatat gcctgctgtg
     6361 gccactactc actttgtagc aaagaagcaa actgaattag atattgagca actactaatt
     6421 tatccgaatc ccctatccaa ggatacctat atcactttta tacttacttc tgatgcacag
     6481 gtaaacattg gaatttatac cttgcgaggt aaacgcatac acaatcttaa ggtatatggt
     6541 aagcagggct ttaataaaat ctattttagt ggaaaggacg accaaggtga tacattatcc
     6601 aataacacat actttttaaa agttcaggcg acttccgcag atggcaaaaa agtagaaaag
     6661 actgaaaaaa tggtcattta taaataggat aaaaagatat gttactgtat aatacgcaaa
     6721 cccgaaaaaa agaaaagttc atacccataa ctccaggaaa agtaggaatt tatgcctgtg
     6781 gtcctactgt ttataactat tttcatatcg gcaatgccag ggctttcatc ttttttgatg
     6841 tagtccgccg ttattttgaa tatcagggtt atgaagttac ttatgtacaa aatattaccg
     6901 atatagacga taaaattata gcacaagcaa atgaagaaaa tactagtttt catatggtag
     6961 ctgataaata cactcgcgct tttctggaag atattgctgc cctgggaatt aacaaaccta
     7021 cttatcaacc caaagctaca gaagttatga atgatataat tgctttcata gctgctttgg
     7081 agcagaaggg ctttgcatat tctgtggaag gagatgtata ttttgctaca gagaaattgc
     7141 cttcctatgg ctgtctttct ggtaaaaaaa tggaagagca gcttgctgga gccagagtag
     7201 tagaaaaccc taataaacat catcctgccg attttactct ttggaagaaa ggtaaaccag
     7261 gtgaacctgt ttgggatagt ccctggggta aaggacgtcc tggttggcat actgaatgcg
     7321 tagttatgag caaaaaatac ctgggtgata catttgatat tcattgcggc ggaatagatc
     7381 tcattttccc tcatcatgaa aacgagctgg ctcaggcaat ggctttcagt gataaaccct
     7441 tatccaatta ttggatgcat aatggctttt tgaatgtaga cggagaaaaa atgagcaaga
     7501 gcttgaagaa tttcttcact gctcgtgata ttttggcaca ctacgacgcc gaggcaattc
     7561 gtttcttctt tctatcaaag cattatcgca gtccgattga ttttagccgg gaacttattg
     7621 aagaagcaca acgagctgtt gccaatttat atagctcact ttcggaaatt aattatctgg
     7681 ataatgttca aaactcacct caaacgatgt gtggaacagc agtagagtta caaaatttac
     7741 ataaagaata tatcacagct atggatgatg attttaacac agctaaagct atttcgatcc
     7801 tttttgaact gaaccgtaaa gttaaaaata catcttcagc ttttgcagac cgccttgctg
     7861 ctgctcaaaa acttgtagaa ttaggttcta tcctgggctt cttccaaaat ttggaagaac
     7921 aattaagtac atttatgcct gatttgtcta aagacctgat cgagctaatc ctttcctacc
     7981 gccaggaatc aagaagaaat aaacaatgga ctgtttctga tagaatcagg gatgatcttg
     8041 ctcgccttgg catagaaatt aaagatacgc ccgaaggggt caaatggaat ttaaaggaga
     8101 aaaaatgaag aataagactc tctaccttgc tcttgttctg ctaattgtag tagcaattat
     8161 tatcgttgtt ttgctaatta gccgaaaagc acctttaaac ggacccgaga gtattgccta
     8221 cgacgatgtt agtaaacaat ttttggtttc caatacttta agtaaaaaca tcgtttctat
     8281 gagtttaaac ggtcaattta ctcctttctt gaaaaaagga ttacaggaac ctcgcggtat
     8341 tattatttat tctcataagc tatatgtagc cgataaggcc tctattaaag tgatagattt
     8401 ggataaagca gaaattattg atactataaa tatccccgga gcattgatgc tcaatgacct
     8461 agcttttacc aaacagggag ttatttatac cacagataca gctggaaatt gtgtttatgt
     8521 tatagatacc cttagcaata agatccaaaa gatagctaat cctttgctgc aaaagcccaa
     8581 cggtattatc tatgatatgc ctcgggatca aatgtttgtg gtctgttttc gtgaaaaaag
     8641 ccccatttta gctatcagta ctttggataa tagcattagc atttttatgg atagtattta
     8701 ttccgattta gacggcatag ctattgatga tttaggcaga atttatatta gcagttgggg
     8761 tcaaaatatg atttttgaga ttccccaaga acaaaatcgc tttttagcca catttacgga
     8821 cattgaagat gctgctgata ttttatatta tctgccgaat aatgaattaa ttgttcctct
     8881 gttcaataag aataaaatca tccgcttaaa gcttgaataa aaaagattct acctatctaa
     8941 ttattctgcc tgattgtgtg atcaagcatt ttaatgaccc gaagcacttt caggcagaac
     9001 tttatgcata tcaagctgga ctgagaatgt tgcctgatct tttagatttt caagtaccgc
     9061 aatggattaa aattgaaaaa gtaaagggca ttccctattt ggatagactg gattactttt
     9121 atcctgaact tcttgctgaa acttatgcta actttcattt agctacactt aaaaacgata
     9181 aatgcctctg ccacgtcgat aattcaccta agaatatcct gttctgtcaa ggaaaatatt
     9241 attttatcga ttttgctgat agtagatttg aatatccaga aagggatatt actcatcttt
     9301 tacttttttg ggctgccgat tttcctgctg attattttaa gtatgcgctt gagacttttt
     9361 tgaatggata tctctctttt gtaaccttaa attctgaacg ctggaattct tacttaaaaa
     9421 gtaacattat tctttttgac caaagaagaa aactccacag caaaaaaacc ggaaaagaat
     9481 ccccttcaat ccagaattta aaccgggaac ttttagccca aatgaagctg tgttatatga
     9541 aagagtgaac ttgtttttat gattttcccc catatcggat cgatatttcg tttattttaa
     9601 aataacctaa gcttcagctt cgaatagaaa aacagggtaa taattatatt tttgctaaat
     9661 taacctcgta tcaatagaat caagaattgg cttttaactg aaaaactaca ataagaaact
     9721 aaatattcaa cgctcgttgt ttttagaatt gcgaatagag atcagttctg ccataatact
     9781 tacagcaatt tcttcagggg tatttgagcc aataggtaaa cctataggcg aatggcaacg
     9841 gaaaagttct ttctctgtaa agccctcttc ttttaatttg gcaaaagtat caagcacctt
     9901 ttttttacta cccatcattc ctaaataacg gaagttagtt cttaagcact gttccaaaac
     9961 ttctttatcg tgcaaatgac cttgagtcat aataacaatc cagctatccg aatcaaaatc
    10021 tataatctct gaaaggtctg tataattatg aagaatagca gtgtgataac aatcctcaag
    10081 taaattatca ataatttctt tgcggttatc gatcagataa acatagaatc cgcagagtcg
    10141 agctaatttg cctaatgctt ttccgcaatg tcctgctcca atgatataga gtttatcact
    10201 taaaaataag ggttctatat atattttaac ctgtcctccg caaagcatag aagtggcaat
    10261 tcctgtttta gccccatctg tagataagtt aaagctgaat atttgtgcag tcctgggttt
    10321 ttctttttta atcatctcta taacagtgtg ttctaaatcc cctccaccca gattgccaaa
    10381 aggttcttca tttagagtaa cggctaattt catgccacat ttggctggag ttgaaccgtc
    10441 gctttcaata atgcttgcct gccatacagt tttgtgttct tttagaaaga tagatatctt
    10501 ttcgtaaaaa gtaagattat ccatttggac ctccaaaaat aatctggtag agagcaattc
    10561 ctgttgccac tccaacattc aaagagtttt tccatcccaa ttgaggcaaa gcaatgcatt
    10621 catcacaaag ttctaaaact gaaggttcta tacccagagc ttcattaccg ataacaatag
    10681 ccaaagggag tttatagatg gtttgaaata tactacctgc ctcttctact gtttctaagg
    10741 catatatata cataccagat tcacgacata tagtaattgc atcaatagat gtttgtaaat
    10801 attgccattc aaccaatttt tcagttccca tagctgtttt tggcatattg ctatgctctg
    10861 gagttggagt tattccgcat aacagaattt tcccaattcc taaacactca gaagttcgaa
    10921 acaaggaacc cacattaaaa accgaacgca aattatcaca aataacggtt atttgttttg
    10981 cttttttgcg gagaaaaggg tctgccgata tttttccatc cccgattctt agagacattg
    11041 aaccatcttt tcttaaagat ccttccagat aaaaatagag tttattcata agctgatgtg
    11101 gacttaagcg aggattcagt tcttttaaaa aaaagtgcat tttctgtggt aaaggttcgt
    11161 ctactagttg agagagcttt agcagatgat caattatgga aagacgcttt tcaggattgc
    11221 ttagattctg ctctaagcac aatatcagtt tatgtagcgc ttttatttgt tcctcatatg
    11281 taaatgcttt aaattttgtt gcactgtaac aagcagaaaa cttcataact tgaaatccct
    11341 aatcagattg gcggccgtaa gtaaattttc gcaaacaaaa ttaggtttaa gttcattcgt
    11401 ctttataatc tcgataaaat ccttgtgtcc atttcccgtt aacaagagaa tattctttag
    11461 tcccgcattt ttcccaaagg caatatcact atggcgatct ccaatcatcc aagattgttc
    11521 aatatcaaaa tgaaaatcgg aatatgcttt tttgaacatt ccaattccag gcttacgatc
    11581 ttcgtgatca atattatatg gtggaataat accatcttta taatagggag aataatatac
    11641 tccgtccagt tctattcctc cagcagaaat taaagaacgc atctttgcca atactctttc
    11701 taagtcctct atagaaaaat aacctcttgc aattccggac tgattagtaa ccacaaaaag
    11761 caaaaaatcc aattctttaa atatccttaa tgcctctata gtataaggat aaagatgata
    11821 ctgctcgggc tcgcttatat atccaaaatc atcgggactg ataactccat ctcggtctaa
    11881 aaaaatggca cttttaatat tttctgctgt catgggtctg aaacctcaaa gcatctggta
    11941 ataaggtgtt aaatagtgta attctataat cccaataatc atttcttaaa gtaatattaa
    12001 tatccatttt ccagcaatgc agatctctgg aaatagataa ggtttgagca agcatattgc
    12061 tggtttttaa attataataa ttggtatatg ctaaactcca gttctcagta attttacagg
    12121 ttaagttcat tcttaagtta ctactttcag aatggaatat gtccttatcg gcataaatat
    12181 tttggctgat ggaaaagctc cagtcattgg aaccagtatc ttcttgaaag ctctttggat
    12241 atgcatttaa cgaatcgtaa ctttcaaata cttcgaaatt tctgtttttg ggagcaacaa
    12301 aatatttagt ataaggtgct gaaccgctaa ttgatattcc ctgagaaaaa tactgatttc
    12361 tcaagttcat atcgttccag tgaatttgat agggattctg agtagcgcta aattgagatg
    12421 aataaccaac ttttatgtct ttaacttggt attttccatc attgatagaa agcgtgccta
    12481 ttttatatgt ccctggtttg aaagttaaat tatgcgaaat attagaaaag ggctgatcct
    12541 tcttcttaag atttgcagat gtacctatcc tccaatataa caagtcattg agctttgttt
    12601 ctttaccctt tgttccatac ttaagctgcc aattgttatc cacactgaaa tttagattag
    12661 atgattcctt tacactgcta accgctatgc tgccaaaatt atataaatca gagttttttc
    12721 gcgtatccgg ctgataggaa taaccgatat taggagtaat aatatgacgg acagatgtta
    12781 ctggaaaatt actaaaattt cttaatccat aaagattgaa tctggcattt acagatgcag
    12841 tccaggcatt tcctctggca tatttatcat tctgtttatc tctatccatc caagcttccg
    12901 aatagtcaaa actttcccct aatgttaacc aaccaaataa tttggaatta cgcgaaatcc
    12961 ctgcattatg ctttatacca aaatgatgct catttagcat tgttagtcct gtggtatctg
    13021 aaggatcaac cgtattatcc caaaacaaat cgcttataga gtaattcttt tcttttaaat
    13081 atcctgtatg ctcaagataa gcattatacc ggtagttgaa attgctaagc caagaatcgg
    13141 atgaaacatt taaaagttcc gatacgggac ggcttgctaa ataaaatgag gcacttggta
    13201 aagataaagt agctgtatca tttattaaat cctgagtaaa attactacct gcattaaaat
    13261 aagaagaacc aatcggtttg gaatacgaaa cgctggaagt taatctttga gctaagcttt
    13321 ggtcaacatt actgctactt tcccaaattc ttttattact tacaaaattg atgttggcat
    13381 ccaaagatga tttttctgga agatcttggt gatgtctacc ctgcaaagac caatcatcat
    13441 aagtaacatc atcttttaag ttatgttgat aagaagcatt caaattacct gaaaaagaat
    13501 agcgttttag ataactggta tcaaaatgac ctttccagcc agttttctcc ataatatcaa
    13561 aaccaaaaac aaaatctgca tagtctttgt atggataata aacagcaaga tttcttatta
    13621 caaagccatc gacattatta taatctggcg aaggaattaa aaagccagga taccttccct
    13681 gtctgatcgg aatcgtgata aaagggaaaa aaaatactgg catatgattt acatagccta
    13741 taactggttt accaacgatt ttatcgcctt tatacacccg taattgtttt gcccaaaacc
    13801 agaatgaagg ttcctccaga tcacaattag taaatgttcc tccatcaata tcataagtat
    13861 ctttatcggt tttacggagt tcttgtccgc tataataacc accttctatc aaacttttgc
    13921 catcatccaa aataccggtt tgagtattaa catcaaagcg aatattggta ccaagaagta
    13981 actggtcgcc atctttcatt cgggttggac catagctata agcacattcg tttttaatat
    14041 ctattgtgat agaatcggca ataatttctg tatcctgata attaatttga ggtttaccat
    14101 agagttttat tatttctgtt gtatattcaa aatggatgct atccgcttta taaaaaagag
    14161 aatctgtctt tactatgtta atatctaggc tatccggaat ttcaggaaac agcgtatcag
    14221 gcatagcggg gctaatagct tcactttgag cacaaatagg aagcattatt agcattagta
    14281 aggaaaccag aaataaacgg attagggtat tcttcatttt gtaatcaaaa aaaaacagag
    14341 ctatctatgt gtcaagcggg ataataattt ttgcctttaa gccaaagcca attaatatat
    14401 ataaaaaacg ctttttttaa aggagataca aatgccttcc tgtaaagcta cattatattc
    14461 tttgataagt atcctgattt tgctaagctt aactgcctgt aaaattgata tgaaacagga
    14521 aatttggctg aaaaaagata attcaggaaa ggcatctgtt atagtacata tgcagattcc
    14581 ttttggccta caaaatgaaa ccctggactt gcaggaactt aattctttca atgctctatc
    14641 ttcacttgcc gaagttgcca gaaaaacacc tggtgtatca gttaccagat tagctcaaga
    14701 agaaactcat aataagaaag aaatgaattt gtcttattat ctggatttta cctttaaaga
    14761 tgtagaaggg ttgagcacag ttttgagcgc ggaagggaga aatgctatat tccttacaaa
    14821 atcaggcaag cataaagtgc tatgcataaa agcagaagaa cttgccctca tcgaagatga
    14881 tgattctgac gaatttgatt ttcttagctt tatcgaaata aatttgcgaa ataaggttaa
    14941 tctgccggca agggtaaaga aaatagataa cacaggttat gggaaatata agggtaaaac
    15001 tgctacttgg gatattgagt tagatgacga ttggcttagt tgtaccagcc atctattata
    15061 tgttcatttt taagggggat ttttgaaatt aacactctgg tcttggaatg taaatggatt
    15121 gcgagctgcc ttaaataaag gttttattaa aatcattcaa aacgaacaac cggatatctt
    15181 aggaatacag gaaacaaaac ttcaggaaca tcaaattcct gaagaactaa atactttagc
    15241 gaactatctt atttattggt ctcatagtat ccgaaaagga tattccggca caggattatt
    15301 tacaaagata ttaccgatta gcttttccac tagttttggc aatccggaat ttgacagcga
    15361 aggtagaata aacattgccg aatacgataa atttatctat ttcaatatct atttcccaaa
    15421 cggacaaaag gatgatgaac ggctaaatta taaactgcgt ttctatgatc gctgtctgga
    15481 aatgatggaa gaaaaaagaa caacgggcaa gattatttta gtgggtggag attttaatac
    15541 tgctcacaaa cccatagacc tagccaatcc gaaagaaaat gaaaaaacta gtggatttct
    15601 acctattgaa cgtgcctggc tggataaaat cgaaactatg ggctgggtag atactttccg
    15661 tcattttgat aaaagtccgg aacaatacag ttggtggagc tatagaacaa aggccagacc
    15721 aagaaatgta ggatggcgtc tcgattattt ttgggtgaat caagaaggtc tgaaacatat
    15781 tataaacgca gggatcagac aggatattga gggttcagac cattgtcccg tatttgtaga
    15841 actggaaata taagctgtat atttaaactg tgcttatcta ctttactttt aatttaatac
    15901 catctgcaaa ataggaacgg ttaaaaccaa aacatagatt gtaatcaccc ttccctgcag
    15961 gaaaatctat tattacttca aattgtttat cggttatcca gtggattgga ataggtttca
    16021 atcccaaggt ataatactga tcatctatat tatacttgaa attagattca ggaaagttta
    16081 gtattgcctc taattcattt accggttcga gagacataaa ttcaaactgt agctgatatt
    16141 tatttttcac ttcaagctgt tcaggaatct gcgaaagcaa cttagctata ggtgttccaa
    16201 aattctgagt agcatacaaa acactccctt gggtaaacac accaactcct aaatgagtga
    16261 aagagccatt taagatattt tctctatgtt cagtagaatt catccaaccc tctactattt
    16321 cttctggagt aaagattttt gctccgctca cgattttagc taaattttcg ccaatagagg
    16381 caactattaa ttgcggatag tatcttcttt tacgatcatc aacaagatag ccaagatggt
    16441 ctttatgtgc aaagaaatca tatttaaaca tattactact gtgcatacgg gccaaatctg
    16501 ccaaaccctc atcatataaa agaggagaaa gattatgtgc tattcgttcc tgattagtca
    16561 gctcccatat tctttgttca aattgagaaa tactaatggt tttggacgat tgagtgcagc
    16621 tcaaaagtaa taatataaga agtaatagaa attttctcat ctatcctcga tcaagctaaa
    16681 attcatattc taaaagaaga atatctttct caaaacgaag atgtactttt tccttatcag
    16741 aattaaaatt tgatagatgt gcatctatat atgcatcgag agtggaaaaa gccatagtta
    16801 accccatcaa ccaaatatca cgcgtgcgtt tttctttata gtttttttgt ttgcttttgt
    16861 aactttcgat tagtatagga tcagtagtaa tgctgatttt atccttataa tcgttaacct
    16921 tatcattatt atgaatggca gtagccagca aataactttg aaatccaatt acagctccag
    16981 cttttatgta tgcttcattg taaatctgac cccctccggg aaatagagaa tatataacag
    17041 ctttggtagg atccttatta accggttgag ctaacaagca accgcttatg gttaatgtga
    17101 ttaacgctaa tattcttcgt ttcaagataa ctccccttta gcttttttgg ctttttcttc
    17161 ttattccact attttgtcaa taacaatatg ctggagaata aaaaagtgca accaaaggga
    17221 ttctttcact ccataaaaaa aaggagctgt taaagctcct aatataaaaa atggtgcaga
    17281 aggcgggact cgaacccgca cacccattac aggcacaaga tccttagtct tgcgtgtctg
    17341 ccagttccac cacttctgct tatttattta gtaggtactt gtttaggaat tggattagcc
    17401 gcaggagctt gctgttccgt atctttaatc tttttttggc gttcttgaat tcctgtaaga
    17461 gcactgctgt ttatatctct aaccaaaaaa gcaagcagca cacaggaagc agcaaaaaca
    17521 acagcaagta tctgagtcca cttttttaga aatttagagg caccactgcc cccaaaaaca
    17581 ttcattgcgg ctccacctaa attggaatcc aacccgccct tggaagtttg ggctaaaatc
    17641 accaatacta gggcaataca aattatcaca tggataatta aggcaatcga gtataaaacc
    17701 attatttagt ccttattaac cttataatct ctttagaatt ttacaaaaaa ttgtatgtcc
    17761 tctttctgtc aatcaaaata atcggtagca tagaaattga aacttgcttg agtgctcatt
    17821 tatagttatt atatacatta ttaccatatg gaaaagcgat aaatagaaat agacatattt
    17881 ttcaaggcat tcccacaaaa aagtgatgtg gacaagttat aacacactgt tg
//
LOCUS       DAOSTH010000027        17823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3292_length_17823_cov_15.329581, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000027 DAOSTH010000000
VERSION     DAOSTH010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 17823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17823
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3292_length_17823_cov_15.329581"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            181..1224
                     /locus_tag="PLB65_04830"
     CDS             181..1224
                     /locus_tag="PLB65_04830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HOQ77887.1"
                     /translation="MKRYLYLAVLIGLALILVSCWFTEPIYLLPESWLCSVNADGTGF
                     RKIKKVDLDFGTTGFWDIYMTKDNRIIFYGEKLWISDTDTIRVEQITPDNLTLTHLPS
                     RLSQSPDGSKLYFAADKNIYQLSYPDYQLTQLTHQTTRWLRNPIVSDLGNYITYSSDG
                     FGYPTKETEYMYWLNLINGQSDIIPSPDTLSFNPYYSEIEDYVYYERAGLFRSRLDGS
                     DRTTVDNYGGSGEPYTMFSISNDQRYIVHKSNPTYNNYFVRIFDKVCASGVNIPVKNS
                     PPYELLGRLCKEANIVFYVSPGYPEKLHSYRLDTGEDTILNPNQVNIANTYIIAPTWD
                     GSKVYFYADIEVK"
     gene            1234..1521
                     /locus_tag="PLB65_04835"
     CDS             1234..1521
                     /locus_tag="PLB65_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77888.1"
                     /translation="MKMLKIFLWSIMLSVAFFLFADNEADSIAAYSIEWKSTGSNAVI
                     DSVFTIDVTAIPDTMTYKFEGNDIPIPDAQTGDGIDNSQIIRFDTSLLIVV"
     gene            1844..5005
                     /gene="secA"
                     /locus_tag="PLB65_04840"
     CDS             1844..5005
                     /gene="secA"
                     /locus_tag="PLB65_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOQ77889.1"
                     /translation="MLEKVLKKIFGDKNIQDLKHYEPVVREINEIYSGLEQYEDEQLI
                     NRIQEIKQEIADKLNPLRNKLSDLEKEYRETREDNERNRLDNSIDTTKKELKLVTKNT
                     VDDYLPEVFAIVKDTCRRMLGQKFEVRGHEVEWNMVPFDVQLIGAMALHDGKIAEMAT
                     GEGKTLVATMPLFLNALVGRGVHLVTVNDYLASRDAEWMSPIFNFHGLSVGCITTGMD
                     FDSRKEAYNCDVTYGMNSEFGFDYLRDNMAVSPEQLVQRDFYYAIVDEVDSILIDEAR
                     TPLIISGPIAQDKNFYRELRPLIAQVVQTQNALVQRYLSEINEDLNDNNPNADNNRLA
                     KNLLLLKRAAPRNKAFMKLMQDGELKKMVNDIEGIYLRDKKMNELDDNLFFVVEERHN
                     SVDLCEKGREMLSKKDKDLFVVQSLDEILAEIDNNEALSEEEKQQQKAIQTNRFMDKS
                     EKLHNINQLLRAFTLFENNQEYVVIDNKVIIVDEFTGRQMPGRRFSDGLHQALEAKEN
                     VEIEAGTQTFATITLQNYFRMYDKLSGMTGTAVTEESEFMEIYNLPVMVIPTNVPITR
                     IDHDDLIYLSKNDKYQAIINEIIYWHEKQKPVLVGTVSVEVSEIISRLLKRKGITHNV
                     LNARQHQREAEIITEAGQPGAVTIATNMAGRGTDIKLGKGVVEGPYESYVGLPKTLTE
                     EYPYGLPLDGLHVIGSERHESRRIDRQLRGRAGRQGDPGTSRFYLSLEDDLMRLFGSD
                     RIAPMMVKMGLKSGDVIRHPWMTKAVEKAQKRVEEHNFEIRRELLKYDEVMNQQREVI
                     YSYRRSVLKGYDLKNEILEMITDTIVNMLDEYIPPHSYPEDWNLERICQWFQRNLNLG
                     LNVKDIFSDHLNRELLENTLLEYALSAYENKERQIGSEQLRNIERRALLEVVDDEWRD
                     HLHEMDLLKDGVYLRAYANKDPLIEYKKESYDLFQGLIARIQENVSRKVFTTYVLSRE
                     QMENFLKNANLTHQDLNAFLKAQQEQQMNQNMTAPPQYNNPGGEIEKVRPVKVAPKVG
                     RNDPCPCGSGKKYKKCCGMYDTE"
     gene            5043..7262
                     /gene="topA"
                     /locus_tag="PLB65_04845"
     CDS             5043..7262
                     /gene="topA"
                     /locus_tag="PLB65_04845"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HOQ77890.1"
                     /translation="MSKGLIIVESPAKAGTISKFLKNKYNVKASMGHIRDLPKHELGV
                     SVSQNFKPDYIMDKSKSKVISELKEATLAADSVFLASDNDREGEAIAWHLTEALKKEL
                     QNKPVYRIVFNEITSQAINEAIKNPGKIDMAKVEAQQARRILDRLVGYEISPLLWKVI
                     TKELSAGRVQSVALRLICEREAEIKAFVPKEFWKVEGDFWKDNLPPFKAVLEKINGKK
                     VEIPDEKSAIEIVDSIQNAETKLKEIKRSNRNIDPLPPFITSTLQQEASKILGFQAQK
                     TMSIAQVLYEGIDLGGESTGLITYMRTDSTRMAEEAVTKAKDLIKERFGADLVNPKVR
                     VFKNKQSAQDAHEAIRPTDPFRTPESIAQYLTKEQLKLYTLIWQRFIATQMKPVKLLS
                     TNVKISMGNAEFTSTGAQIIEEGFLKAYSYINTPLGEKIHPDYTKDDELQHNALEKTQ
                     HFTTPPSRFTEASLIKELEAKGIGRPSTYASIIDTLRKRKYVNMEKKSFIPTPLGNDV
                     NRFLVDKFDCLFNVKFTAEMETKLDEVEYSKIAWNDVVKEYYDQLQALINQVDIKKEK
                     NNFIQDTGIACDVCKQGTMLIKHSKRGEFLACSRYPQCKNSKSFTRDADGNIKIVVPT
                     PLEEKCPECGSPLMLRNGKYGEFIACSNYPKCKYSRPKTLGIKCPECGIGELTAHKSK
                     KGRPFYSCNRYPECKYITNDKPLPISCPKCGNPYLVEKYSREKGKYKMCPKCKTEME"
     gene            7266..8537
                     /locus_tag="PLB65_04850"
     CDS             7266..8537
                     /locus_tag="PLB65_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ77891.1"
                     /translation="MNIKDGINSAFQSIFSHKLRSLLTLTGIVIGVLAVVTMFSSVYA
                     IKGLIKKNMEGMGWDNSIIIYPSVGNSDKEIGKNRSHIRRPKQSVPFLNYDDYLALKE
                     NLNYKCIYGSISKQSLYRVGNKVNNIILRATEVDFFKNKSYPIGKGRYFNAYEAENNI
                     PVAVLGYQFAEEYFKDKDPIGQVLALGSHRFTVVGVLAPDVLNSGNGMNFNPWERQWD
                     LKAVYIPLKYGATYIEPNKIIHQIYLQSYSTEDYLKLKNEARQIMLARHNMYPNFQFM
                     DIGDMILKITSEINKFMDKWNITLIAIASISLIVGGIGLFSTLLISIQERMTEIGIRK
                     SIGATEQDIFFYFIFEALALAFTGAVIGVILAWILITLIASAIKFPLYLPMQGVVVGI
                     GFSLLIGFLSGLYPAWKATGIDPIQAIYYRE"
     gene            8678..9256
                     /locus_tag="PLB65_04855"
     CDS             8678..9256
                     /locus_tag="PLB65_04855"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOQ77892.1"
                     /translation="MKIINKITMFLAKKRLLITRVLCIAVILEFILNFHYRIEHIITN
                     PTAIIISIILIVLGAFIRSWSAGIIEKRNRLVMEGPYALCRNPLYAGSSLILLGAVLF
                     VNDIWAWLLAGLLILVIFPFTIKKEELTLSTNFPEDWKNYTATTGRYFPKKLDWQRLK
                     QKWSFQLWLKNREYNMFLFSLLLIIIICVLGF"
     gene            complement(9534..10415)
                     /locus_tag="PLB65_04860"
     CDS             complement(9534..10415)
                     /locus_tag="PLB65_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HOQ77893.1"
                     /translation="MNGLQLITEEHIPLLQDFLTRYPRRNCDFNVTNLLAWGTIYRNH
                     YLLWQDNLVIFNPKYQNVCFPLGDNFTPAELANLVRLFKQEYPEAELIIVPEEYYAQT
                     PELDNYFEVSDDRAWADYVYEIDSLVKLSGKKLAKKKNLISQFIRAYPDYQVLPVTND
                     KLDALLRFTYKWKRERSAEGIYLMTELKAIENTLAMWDILPCEGIIICLHDKIAAYSI
                     FSPQTPDMADEHFEKFDPDKKGSAQIVNWETARHLQNRYKYLNREQDLGMEGLRQAKL
                     SYQPAFLVKFLSTKLKA"
     gene            complement(10416..10835)
                     /locus_tag="PLB65_04865"
     CDS             complement(10416..10835)
                     /locus_tag="PLB65_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ77894.1"
                     /translation="MTQINCKIVKETVATAILALYRQAGWWSTEDKDSDLEIITKIVA
                     NSFCFVIATLDDQIVGMGRALSDGISDAYIQDVTVQKEFRGQGIGKAIIRTLVSFLKE
                     HKLQWIGLICEPGYEGFYQGLGFKVMASYTPFLYSET"
     gene            11139..13979
                     /locus_tag="PLB65_04870"
     CDS             11139..13979
                     /locus_tag="PLB65_04870"
                     /inference="COORDINATES: protein motif:HMM:NF017539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOQ77895.1"
                     /translation="MIDDELIFGSDFFDLEEELAPTSDKLHLREGELREVTVLFADIH
                     GFSSISNLFDAETIHRRMDEIMKIFSRCISFYGGFVDKYMGDGIMALFGAKTATEQNT
                     ERAILAALKMQEQLQLYNKKLKQQPDFENVDLGVRIGINAGLVSVGKVGEDREGDFTV
                     YGPEVNLASRMESNAPVNRIMLPEKTMLAVQRNFEFEDVGLKSVKGFSEPLPCYLVIG
                     PKLDSSLHRRNLSTRYIGRIKEIDLLKETFSEIGKQQQISVVAIQGEAGIGKTRLVYE
                     FERTCADNTIFLQGACSAISPTPLNLFTSLCETLFRLQINEKPEVKRKKLDNAYNILW
                     DIASEEEKELLSDGKNLIAFLLELKSEDSRLKQSGADLLQHLSLAIENLIMAQINYAL
                     RSNRKLVIVLDDLHWLDEASAKVMENLLNKIASAGALPPLMLILMFRPGYNLASYFQV
                     LTQVKIIELNPLAAEEIRELIAAQAKDKHLLEDTVDKVIQLSQGNPFFVEEWCNYIED
                     LPLDELKDIPVPANLYNLILSRLDKLPVSLRMMLHKAAVIGQEFFVEILRYMEKRLQD
                     NLDVDATLKSLEDQSYILHSLGFDYSTYFFKHILTREVAYQTLLAENRKVLHRLCGEA
                     IETLYPDRLDEFYYALAEHFHKAEVIDKAIIYIEKAANSAAKVYNNQQALRFYQLLLD
                     YPDLQPVKRLEITMQIADIMWLTGDWNNALKIVYDLLHEAQKINAEKVCFEAYRFLGI
                     AAFYQNEKDEALSYFKLAYDLAKKLNDPALISIAVGNLGNWYFRTGDLTLASHLQQQD
                     LSISIDRQDSKRQAKAYSNLGLICLEEDDLEKAEDYFYKSLKIAVDNRFLKEQSIALG
                     NLGYTKMLAENYAEAFPLLQQKLKIATEMNDKLELINVLGNIAIYYMEIGERNMAMEN
                     YHKIVDIYHYLGDEKGEQEIRDYIALLNSELK"
     gene            14191..16305
                     /locus_tag="PLB65_04875"
     CDS             14191..16305
                     /locus_tag="PLB65_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HOQ77896.1"
                     /translation="MKYIVTALMIVICLCPLQAMRKALVIGNANYAEGILHNSINDAT
                     DIAATLKDLGFTVSLHKDLNREGFYKAVGDFVSALVSSDEAVFFYSGHAAQIEGTNYL
                     IPINEHIDSATRCAYLSYNTSMLLEELSRAAVSIVILDACRDNPFEFSRSLRSGLAPM
                     KAAAGSQYVIFATEEGKTAVEGENRNSPFTESLLANMRTPGLKITDFVQIVSNEVAIK
                     TRERQIPYSTGILRQDFYFAKAEPITPAPRISDNLPVEAKTFTPHGVGSIIVKTSLGG
                     DIYLDGNFIQSLFAGNQATWTDIPVGIHEIKLVNPRESVIRNLKVEYNQTAVLDFDTK
                     EIAKDKIEEKPVLGSINTNSLSPEPHFVKEPPLPIVNNATNGTGTLIITAAYECDVYL
                     GGQFIGKVEAGQKCKFAKENAGVYTLELMHRSLFCRREIKIHNKETLNLILTTSDYIP
                     YPDALLYVSGGTFQMGTYFINRSSEEHPRHEVQLSPFLMSKTEIEQKLWTEVMGYNHS
                     EIKGGNFPVTNVSFYEALEFCNELSVRNGLTPCYIIDKKLLDPNNLCVLDTLHYTVVC
                     NWKANGYRLPTEAEWEYVSRCGDSYNKVQFSGSNNISKVSWYSDNSEDRIQEVATKEP
                     NEWGFYDLSGNVLEWCWDYFDAYNKQKQVNPKGSEKGIYHIARGGSFYRLADASTCTA
                     RFGFASHHKSNELGFRIVRNVH"
     gene            16418..>17823
                     /locus_tag="PLB65_04880"
     CDS             16418..>17823
                     /locus_tag="PLB65_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HOQ77897.1"
                     /translation="MKKLYLILIVLVGTTLLAAERGLTVNEIPVSDEVTTVSTPVPAF
                     FNNESKANKSKLEFPKSWDTLQELNFNYRLPAKQQVGMQFMNLTHNVQPFGYPYSLSE
                     TAFQAINKSPEWLQAELSSVLAQLEPEKQLLWSNLILSTQDPYVDEIAFCIAYSSPQY
                     LNSTFALPELFTENATAIYSLATELPYVEIIDTGNSATGGNYYSTTRYFKKNTNGEMQ
                     QVTVPQEIYYWYIVHPKLTDEIAAYINPTVIENNSNHNNNIAAPPTGKFWRTYLYNLQ
                     EGDYPVLSDTLVQCQTLFNRDGTGNDAIRTIQWWINQQMSFTSNNERPHQPVRIVTKH
                     FGRCGEYADFTAAVARLALIPCTSIISISTDHTWNEFWDENWVAWEPVNGYIDDPLVY
                     ENGWGKVFGSVIEIRSDGLFTPVTDRYSEGLCHITIQVVDSNQRPVDGARVVLAILDG
                     SNVFDCDQYTNNNGIVTFT"
BASE COUNT         5831 a         3039 c         3567 g         5386 t
ORIGIN      
        1 acaagattgt ggatcatcat acccaaagat ttctcggaat cggtgccaaa gctattgcca
       61 gccaagctaa tggctatgta tttaggtaat cctgaagttt ttgaaggtaa cgcaggactg
      121 gtgaaacaaa gtaagtacca aatccaagaa ttccaaagat cagatagata ggaggaagaa
      181 atgaaaagat atttatactt agctgtgtta ataggtctgg ctcttatatt ggtatcttgt
      241 tggtttaccg aaccgattta tctacttcct gaatcctggc tttgcagcgt caatgccgat
      301 gggacaggat ttaggaagat aaagaaagtt gacttggact ttggaacaac cggattctgg
      361 gatatctata tgaccaaaga caatcggatc atcttctatg gggaaaagct gtggatttcg
      421 gatacggata cgatcagggt tgagcagatc acaccggata atctaaccct cacacatcta
      481 ccttctcgtc tatctcaatc tcccgatggc agcaagctgt acttcgctgc agataaaaac
      541 atctatcaac ttagctatcc ggattatcag ctcactcagt taacacatca aactaccagg
      601 tggctaagaa atcctatagt gtctgattta ggaaactata tcacctactc ttccgatggt
      661 ttcggatatc cgacaaaaga aacggaatat atgtattggc tgaatttgat taacggtcaa
      721 tctgatataa tcccaagccc tgatactttg tcatttaatc cgtactactc agaaattgag
      781 gattatgttt actatgaaag agcgggtttg ttccgttcca gattagacgg aagcgataga
      841 accactgtgg acaattatgg tggaagcgga gaaccttaca ctatgttttc gatatctaat
      901 gatcaaagat atatagttca caaatcaaat ccgacatata ataactattt cgtccgcatc
      961 tttgacaagg tttgcgctag tggagtaaat attccggtta agaattcacc tccatatgaa
     1021 ttattaggaa gattatgtaa agaagcaaat atcgtttttt atgtcagtcc tggatatccg
     1081 gaaaaattgc actcatatag attagataca ggagaagata ctattctcaa tcccaatcaa
     1141 gttaatattg caaacacata tataatcgct cctacctggg atggctccaa agtttatttt
     1201 tatgcagata tagaagtcaa gtgaggttga aaaatgaaga tgcttaagat tttcctgtgg
     1261 tctattatgc tttcagttgc atttttctta ttcgcagata atgaggcaga cagcatcgct
     1321 gcatattcca ttgaatggaa atctacagga tcaaatgctg ttatagattc agtgtttacg
     1381 attgatgtaa cagcaattcc tgacactatg acttataagt ttgaaggaaa tgacatacct
     1441 atacccgatg cacaaactgg agatggtatt gacaattctc agataattcg ttttgatacc
     1501 tcattattga tagttgtata attgttaatg caacaataat atacgaggtg ttcatactaa
     1561 gtagtatttt atgctatttt ttttgtctct cactcacttg ggctcctgag aaagattagt
     1621 tgcttaaatg gaaaaatggt aaaacgagtt gaaaaacaaa tgtgaacgca tataaatctg
     1681 cataatatgt gtgagaaagc gaaatctgtg tggaacaaat ctaaacagtg tatccttttt
     1741 tatttgacac aattccacct tcaaaataaa ctgacaaaaa agggactaat gcattaacct
     1801 tggcttgcag agctatttgt aaaccgagta acaaggaaac aaaatgttag agaaagtact
     1861 aaaaaagata tttggcgata aaaacattca agacctgaaa cactatgaac ccgttgttcg
     1921 ggagataaat gagatatatt caggccttga acaatatgaa gatgaacagc ttatcaatcg
     1981 cattcaagag atcaaacagg aaatagctga taaattaaat cccctgcgta ataagctctc
     2041 cgatctggaa aaggagtatc gtgaaacaag ggaagataat gaacgcaacc gattagacaa
     2101 ttctatagat acgacaaaaa aggaactgaa gcttgtaaca aaaaatacag tggatgatta
     2161 cctgccagag gtttttgcta ttgtaaaaga tacttgtcgt cgtatgcttg ggcaaaaatt
     2221 tgaagttcgc ggccatgaag ttgaatggaa tatggtgcct tttgatgtac agcttatagg
     2281 tgcgatggcc ttgcatgatg ggaaaattgc tgagatggca accggggaag gtaaaaccct
     2341 cgtggctact atgcccttat ttttgaatgc cttagttgga cgcggtgtcc atttagttac
     2401 agtaaatgat tatttagcaa gtcgtgatgc tgaatggatg agccctattt ttaattttca
     2461 cggtctttct gtaggttgta taactactgg aatggatttt gatagccgta aagaggccta
     2521 taattgtgat gtaacttacg ggatgaacag tgaatttggt tttgattatt tgcgtgataa
     2581 tatggctgtt tctcctgaac agttggttca gcgtgatttt tattatgcta ttgtggacga
     2641 agtggatagc attttaattg atgaagccag aacacctttg ataattagtg gtcctattgc
     2701 tcaggataag aatttttata gagaattacg tcctttaatt gctcaagtgg ttcaaacgca
     2761 aaatgcttta gtgcaaaggt atttaagcga aattaatgaa gatctaaatg ataataatcc
     2821 taatgctgac aacaatcgct tagcaaaaaa ccttctttta ttgaaacgag cagctcctcg
     2881 aaataaagca tttatgaaat tgatgcagga tggtgagctg aaaaaaatgg tgaatgacat
     2941 agagggcatc tatcttaggg ataaaaagat gaatgaattg gacgataatc ttttctttgt
     3001 ggtggaagaa agacataaca gcgttgacct gtgtgaaaaa gggcgcgaaa tgctttccaa
     3061 aaaagataaa gacctcttcg tggtccaatc cctcgatgaa attctggctg agatagataa
     3121 caatgaagcc ctttctgaag aagaaaaaca acagcagaaa gcaattcaaa ccaatcgctt
     3181 tatggataaa agtgagaaat tgcataatat caatcagctg ctaagagctt ttaccctctt
     3241 tgaaaataac caggaatatg tggttattga taacaaggta ataatagtag atgaatttac
     3301 tggtagacaa atgccgggac gtcgtttttc cgatggcttg catcaagctc tggaagcaaa
     3361 agaaaatgtg gaaattgagg ctggcacgca aacctttgct actattactt tacaaaacta
     3421 ctttagaatg tatgataaat tatccggaat gacaggaact gctgttaccg aagaatcaga
     3481 gtttatggaa atttacaatc tgccagtgat ggtgattcca accaatgtac ctattacgcg
     3541 aatcgaccat gatgatctga tttacctaag caaaaatgac aaatatcagg caattatcaa
     3601 tgaaataatc tattggcatg aaaagcaaaa gcctgtcttg gtcggaacgg tgagcgtaga
     3661 ggtctccgaa attatttccc ggttgctgaa acggaaagga ataactcata atgtactaaa
     3721 tgctcgtcaa catcaaagag aagcagaaat aattacagaa gccggtcaac ctggagctgt
     3781 aactatcgct actaatatgg ctggtcgagg tactgatata aagcttggca aaggagttgt
     3841 ggaaggtcca tatgaaagct atgtgggttt accgaaaacc ttaacggagg aatatcccta
     3901 tggcctaccc ttggatggac ttcatgtaat tggtagtgaa agacatgaaa gtagacgtat
     3961 tgatagacaa cttcgaggaa gagctggccg gcaaggtgat cctggaactt cccgatttta
     4021 tctttctctg gaagatgatt taatgcgtct ttttggttct gataggatag ctccgatgat
     4081 ggtgaaaatg ggtttaaaat ctggagatgt aattcgtcat ccgtggatga ctaaagcagt
     4141 ggaaaaagct caaaaaagag tggaagaaca taactttgaa atccggagag aactgcttaa
     4201 atatgacgaa gtaatgaatc agcaacgcga agttatttat tcttatcgtc gtagtgtgtt
     4261 gaagggttat gacctgaaaa atgaaatttt ggaaatgatc acagatacta tcgtaaatat
     4321 gctagacgaa tatattccac ctcatagtta tcctgaggat tggaatctgg aacgcatctg
     4381 tcagtggttt cagcgaaatt taaatctggg tcttaatgtt aaagatatat ttagcgacca
     4441 cttaaataga gagctgctgg aaaataccct tttggaatat gcgctttccg cttatgaaaa
     4501 taaggaaaga cagattggat cagaacaact tagaaatatt gaaagacgag ctcttttgga
     4561 agttgtagat gatgaatggc gcgatcattt acatgaaatg gaccttctga aagatggtgt
     4621 atatttacgg gcctacgcaa ataaggatcc attaatcgaa tataaaaaag agagttatga
     4681 cctgtttcaa ggtttaattg cccgcatcca ggaaaatgtt tcccgaaaag tatttactac
     4741 ttatgtgtta tctcgagaac aaatggaaaa ctttttgaag aatgcgaact taactcatca
     4801 agacttgaat gcttttttaa aagcacagca agaacagcag atgaatcaaa atatgactgc
     4861 cccccctcaa tataataatc cgggtggcga aatagaaaaa gtccgccctg ttaaagtagc
     4921 tcccaaagtt ggaagaaatg atccctgccc ttgtggaagc ggaaaaaaat ataagaaatg
     4981 ctgcggaatg tatgatacag aataagtgag atagtaagcg ttaattggta aaaaggaaag
     5041 atatgagcaa aggactaatt atagtagaat ctcctgcaaa agcaggaaca atcagcaaat
     5101 ttctgaagaa taaatataat gtaaaagcat cgatgggaca catccgtgat ctgccaaagc
     5161 atgaattggg agtgagtgtt tcgcagaatt ttaaacctga ttatataatg gataaatcca
     5221 agagcaaagt tatctctgaa ttaaaagagg caactttggc tgcggatagt gtttttctgg
     5281 caagtgataa tgatcgtgaa ggtgaagcta tagcttggca tttaacagaa gcactaaaga
     5341 aagaactgca aaataaacct gtttatcgga ttgtgtttaa tgaaataact tcacaggcaa
     5401 ttaatgaagc aattaaaaac cccggcaaaa tagatatggc aaaagtggaa gctcaacagg
     5461 caagaagaat tttagaccgt ctggttggtt atgaaataag ccctctgctt tggaaagtga
     5521 taactaaaga gctttctgct ggaagagttc aatctgttgc tttgcgatta atctgcgaaa
     5581 gggaagccga gatcaaggca tttgttccta aagaattttg gaaagttgaa ggggattttt
     5641 ggaaagataa tttgccacct ttcaaagctg ttttagaaaa aatcaatggt aaaaaagtgg
     5701 aaattcccga tgagaaaagt gccatagaaa ttgtggatag tatccaaaat gcagaaacaa
     5761 aactaaagga aataaaacgc agtaaccgta atattgatcc tcttcctcct tttataacca
     5821 gtactctaca acaagaagca agtaaaatac ttggttttca ggcacaaaaa acgatgagca
     5881 tagctcaagt tctttatgaa ggtattgatt tgggaggaga aagtactggt ctgataactt
     5941 atatgcgaac cgattctacc agaatggctg aagaagctgt aactaaggct aaagacctga
     6001 ttaaagaaag gtttggtgcc gatttagtta atcctaaggt gcgggttttt aaaaataagc
     6061 aaagtgctca agatgctcat gaagcaattc gccctactga tccttttaga actccggaaa
     6121 gcattgccca gtatttaacc aaagaacaac tgaaattata tactcttatc tggcagcgtt
     6181 ttattgccac tcagatgaaa cctgtaaaat tgctttctac caatgtaaaa attagtatgg
     6241 gcaatgctga atttacttct accggagctc aaattattga agaaggtttt ctgaaagcat
     6301 actcatacat aaataccccg ttgggggaaa agattcatcc tgattatacc aaagatgatg
     6361 agttacagca taatgcgttg gaaaagactc aacattttac tactcctcct tccagattta
     6421 cagaagcatc tttgatcaaa gaactggaag caaaagggat tggacgtccc tctacttatg
     6481 cttccataat tgacactctt agaaagcgaa aatatgtaaa tatggagaaa aagtcattta
     6541 tcccaactcc cctggggaac gatgttaatc gttttttggt agataaattc gattgcctct
     6601 tcaatgtaaa attcaccgct gaaatggaaa ccaaactgga tgaagtggaa tacagtaaaa
     6661 ttgcttggaa tgatgtagtg aaggaatatt atgatcaatt acaagcattg atcaatcagg
     6721 tagatattaa aaaggaaaaa aacaacttta ttcaggatac aggcattgct tgcgatgttt
     6781 gtaaacaggg aactatgcta atcaaacata gtaaaagggg agaatttctt gcctgtagcc
     6841 gctatcccca atgcaaaaac agcaaaagct ttacccgcga tgcagatgga aatataaaaa
     6901 ttgttgttcc tactccttta gaggaaaaat gccctgaatg tggttcgcct ttaatgctta
     6961 gaaacgggaa atatggagaa tttatcgctt gttccaatta tcctaagtgt aaatattcaa
     7021 ggcctaaaac tttgggaatt aagtgtccgg aatgtggtat tggagaatta acagcccaca
     7081 aatcaaaaaa aggacgtcct ttttattcct gtaatcgcta tcctgaatgt aaatatataa
     7141 ctaacgataa accacttcca atcagctgtc ctaagtgtgg aaatccttat ttggtggaaa
     7201 aatacagccg cgaaaaaggt aaatataaaa tgtgtcccaa atgcaaaacc gagatggaat
     7261 aggcaatgaa cataaaagat ggcatcaata gtgctttcca aagcattttc agccataaac
     7321 ttaggtctct tttaactctt accggtatag ttattggtgt gcttgccgtc gtaacaatgt
     7381 tttccagtgt ttatgccatt aaaggactaa ttaaaaaaaa tatggaaggt atgggctggg
     7441 ataattccat cattatttac ccaagtgtag gcaatagtga taaagaaata gggaaaaacc
     7501 gcagtcatat aaggcgacct aaacaaagcg taccctttct aaattatgat gattatctgg
     7561 ctctgaaaga aaacctgaat tataaatgta tttacgggtc aatttccaaa cagagcttat
     7621 accgggttgg gaataaagta aataacatta tcctgcgtgc tacggaagta gattttttta
     7681 aaaataaaag ttatcctatt ggcaagggac gctattttaa tgcctacgaa gcagaaaaca
     7741 atattccggt tgctgttttg ggttatcaat ttgccgaaga atattttaaa gataaagacc
     7801 ctattggaca ggttttagct ctgggttcgc atcgtttcac agttgtgggt gttttagctc
     7861 ctgatgtatt gaattcaggc aatggaatga attttaaccc ctgggaaagg cagtgggatc
     7921 tgaaagctgt atacatacct ttgaaatatg gagctactta catagagccc aataagatta
     7981 tccaccagat ctatctacaa tcatattcca ccgaggatta tctcaaatta aaaaatgaag
     8041 ctagacagat aatgcttgcc agacataata tgtatcctaa ttttcagttt atggatattg
     8101 gcgatatgat cctgaaaatc acttcggaga ttaataaatt tatggataag tggaatataa
     8161 ctcttattgc cattgcttcc atttctttaa ttgtaggagg aatagggcta tttagcactt
     8221 tactcatcag cattcaagaa agaatgactg aaattggcat tcgcaaaagc attggagcta
     8281 cagaacagga tatctttttc tattttattt ttgaagcact ggctttggct tttacaggag
     8341 ctgttatagg agttatcctt gcctggatat tgattacgct aatagcaagc gctataaaat
     8401 ttcctcttta tctgcctatg caaggagttg ttgtaggaat tggtttctct ctgttgatag
     8461 gttttctatc tggactttat cctgcttgga aagcaactgg aattgatcct atccaagcaa
     8521 tttactatcg agaataaaaa tagggaagaa tcaacttgtc gtttttttct ccgaacttat
     8581 ttttgggtag ccggaggagc tgttcctctg caaaatatga aaaatgtgta gccggaggac
     8641 gccgttcctc cgcaaatatt aaaaaggaga aatccgagtg aaaataatca acaaaataac
     8701 aatgttcttg gcaaaaaagc gattgctaat taccagagtt ctctgcatag ccgttatttt
     8761 ggaattcatt cttaactttc actatcgcat tgaacatata ataacaaatc ctaccgcaat
     8821 cataatcagt ataattctga ttgttttggg tgcttttatc cgttcttggt ctgctggaat
     8881 aattgaaaaa agaaatcggc ttgtaatgga aggaccttat gctttatgta gaaatcctct
     8941 ctatgcaggc agttctttaa ttttattggg agctgtttta tttgtgaatg atatttgggc
     9001 ttggttgcta gctggtctgt tgattttggt tatctttccg tttacaatta aaaaggaaga
     9061 attaacctta agtacaaatt tccctgagga ttggaagaat tatacagcaa caactggaag
     9121 atattttcct aaaaaattag attggcagcg tttgaagcaa aaatggtcgt ttcaactgtg
     9181 gcttaaaaat agagagtata atatgtttct gttttcgctt ctgctgataa tcattatatg
     9241 cgttcttggt ttctaaaaag gaagtgggga aaagggaatt tacgcataat tttgtaaata
     9301 gatagctgag gcaacttcct tttcaggggc gagtttgttt cttacttaat ttttatcgaa
     9361 agtatacggt tatgcctctc ggcaaaattc cccgagaagt tactacatat atactgtatt
     9421 tcattaagca acaattatcc ctgtgtacta tattatttat gtaatctaca aaatttgggt
     9481 gaactaatct tccagtccca agcttcgtat ttctataatt tgctctgggt ggattaagct
     9541 tttaatttgg tagaaagaaa tttaaccaaa aatgctggct gataagataa tttggcctgt
     9601 cttaaacctt ccatgccaag atcttgctct ctattcagat atttataacg attttgtaaa
     9661 tggcgagctg tttcccaatt aacaatttgt gctgaacctt ttttatccgg gtcaaatttt
     9721 tcaaaatgtt catctgccat atcaggggtt tgaggtgaaa atatagaata ggcagcaatt
     9781 ttatcgtgca gacaaataat aataccttcg cagggtaaaa tatcccacat tgctaaagta
     9841 ttttctatag ccttaagttc cgtcatcaaa taaatacctt cggcactacg ctctcttttc
     9901 catttataag taaaacgcaa taatgcatcc aatttatcat tggttacagg caaaacctga
     9961 tagtcaggat aagcacggat aaattgagaa ataaggttct ttttcttagc cagtttctta
    10021 ccggatagtt ttactaagct atctatctcg taaacataat ctgcccaagc tctatcatcg
    10081 ctcacctcaa aatagttgtc caattctgga gtttgagcat aatactcttc cggaacaatt
    10141 atcaattccg cttccgggta ttcctgctta aaaagacgaa ccaaatttgc taactccgca
    10201 ggcgtaaaat tatctcctaa agggaaacag acattttggt atttgggatt gaagataact
    10261 aaattatcct gccaaagcag atagtgattt cggtaaattg tcccccaagc aagaagattg
    10321 gtaacattaa aatcacaatt tcttcttgga tagcgggtta aaaaatcctg caaaagaggt
    10381 atatgctctt ctgtaattaa ttgcaagcca ttcatctatg tttcgctata taaaaatgga
    10441 gtgtaggagg ccatcacttt aaagcccaag ccctgataaa aaccctcata acctggctca
    10501 caaattagcc ctatccattg cagtttatgt tcctttaaaa agctaactaa agtgcggatt
    10561 attgctttgc cgattccttg ccctctaaat tccttttgaa cggttacatc ttgaatataa
    10621 gcatcactta taccatcgct taaagctcgc cccataccga caatctggtc atctaaagta
    10681 gcaataacaa aacaaaacga attagctaca atttttgtaa ttatctccaa atcggaatct
    10741 ttgtcttcag ttgaccacca tcctgcctga cgatagagtg ccaaaatggc tgttgcaact
    10801 gtttctttaa caattttaca gtttatctgt gtcataatta tatgtttgcc ctatatacat
    10861 tgtgtagggt ctttccttaa aaaaactatc tggatgaagg gggatttttg tcaagcacgc
    10921 attttaaaga agaaattgtt tcttgcagat tacgcagttt tcacagatgt attttttagc
    10981 catcgaaaac accgaacaaa gaaagaaata cttagctttg agctcccaag ctatatattt
    11041 tgctcttcaa cccaaccctc ttcttttcag tttgaggttg acagaatata tatattcagc
    11101 tattattgta taatgagaat aaaaaggata gcagcataat gatagatgat gaattgattt
    11161 ttggcagcga tttttttgat ctggaagaag agcttgctcc tacttcggat aagcttcact
    11221 tacgagaagg tgaacttagg gaagtaactg tactttttgc agatatacat ggctttagta
    11281 gtatatccaa tctttttgat gccgaaacca ttcaccgcag aatggatgaa attatgaaaa
    11341 tattttcacg ctgcattagt ttttatggtg gttttgtaga taaatatatg ggagatggca
    11401 taatggcatt atttggtgcc aaaactgcaa ctgagcaaaa tactgaacga gcaattttag
    11461 cagcgctgaa gatgcaggag caattacaac tatataataa aaaactaaaa cagcagccag
    11521 attttgaaaa tgtagatttg ggggttcgga ttgggattaa tgctggtctt gtatctgtag
    11581 gaaaagtcgg cgaagataga gagggagatt ttacagtgta tggtcctgag gttaatttag
    11641 cttcaaggat ggaatccaat gctccagtga atagaattat gcttccggag aagacaatgc
    11701 tggcagttca acgcaatttt gaatttgaag atgtaggttt aaaaagtgta aaaggttttt
    11761 ctgaaccgct tccctgttat ttagtaatag gccccaaatt ggatagttct ttacatcgca
    11821 ggaatttaag tactcgttat atcggcagga taaaagaaat agatctgctg aaagagacct
    11881 tttctgaaat tggcaaacaa cagcagattt cggttgtggc tattcaaggg gaagcaggaa
    11941 tagggaaaac acgcctggtt tatgaatttg aaagaacctg cgcagataat acaattttct
    12001 tacagggtgc ttgcagtgca attagtccca ctcctttaaa tttattcact tcgctatgtg
    12061 aaacactttt tcggttgcag ataaatgaaa aacctgaagt taagcgaaaa aaactggata
    12121 atgcctataa catattgtgg gatatagctt cagaggaaga aaaggaattg ctttctgatg
    12181 ggaaaaacct gattgccttt ttgctggaat tgaagagtga agatagccgt ttaaaacaga
    12241 gcggagcaga ccttttacag catctttctt tagctattga aaatttgata atggctcaga
    12301 ttaattatgc tttacgcagt aaccgcaagt tggtaatagt tttagatgac ttgcattggc
    12361 tggatgaggc ttctgcaaaa gttatggaga atttattgaa taaaattgcc tctgcaggag
    12421 cgcttcctcc tttgatgcta atattgatgt tccgtccagg atataatttg gcttcctatt
    12481 ttcaggttct aacccaagtg aagattatcg agctaaatcc tttagcagca gaagaaatta
    12541 gggaattaat agctgcccaa gcaaaggata aacatctttt agaagatacg gtagacaagg
    12601 tgattcaact ctctcaaggt aatcctttct ttgtggaaga atggtgtaat tatattgaag
    12661 acttacccct agatgaatta aaggatatcc cggttcccgc taatttgtat aatcttatct
    12721 tatcgcgttt agataaactg cctgtctctt taagaatgat gttacataaa gctgcagtga
    12781 taggtcagga attttttgtg gaaattttac gctatatgga aaaacgcttg caggataatc
    12841 tggatgtaga tgccacttta aaatctctgg aagatcagtc ctatatttta cattctttgg
    12901 ggtttgacta ttccacttac ttctttaagc atattttaac ccgcgaagtt gcctatcaaa
    12961 ctttactggc agaaaaccgt aaagtgcttc accgtttatg tggagaagca attgaaacct
    13021 tgtatccaga ccgtttggac gaattttact atgctttggc agaacatttt cataaagcag
    13081 aagttataga taaggccatt atttatatag aaaaagcagc taatagtgca gctaaggtat
    13141 ataacaatca acaggctttg cgtttttatc aactactttt agattatcct gatttgcaac
    13201 cagtgaagcg tttggaaatt acaatgcaaa ttgccgacat tatgtggctg acaggcgatt
    13261 ggaataatgc tcttaaaata gtttacgatt tgttacacga agcccagaaa ataaatgctg
    13321 aaaaagtatg ttttgaagca tatcgttttt tagggattgc tgcgttctat caaaatgaaa
    13381 aagatgaagc cctaagttat tttaaacttg cttatgatct tgctaaaaag ttaaatgacc
    13441 ctgctctaat ctctattgcc gtagggaact taggaaactg gtattttaga accggagatt
    13501 taactctcgc ttcccatttg caacaacagg atctatctat atctatagat agacaggata
    13561 gtaaacgcca ggcaaaagca tatagtaatt tggggcttat atgcttggaa gaagatgatt
    13621 tggaaaaggc agaggattac ttttataagt ccctaaaaat agcagtagat aaccgctttc
    13681 taaaagaaca atctattgct ttagggaatt tgggttacac aaaaatgctg gctgaaaact
    13741 atgcagaggc attccctctt ttgcagcaga aactaaaaat tgctacagaa atgaatgata
    13801 agctcgaatt gatcaatgtg ttaggtaata ttgctattta ctatatggaa atcggagaaa
    13861 gaaatatggc aatggagaac taccataaaa ttgtggatat ttatcattat ttgggagacg
    13921 aaaaaggaga acaagaaatt agagattata ttgctctgct aaattcagaa ttaaagtaac
    13981 atattttgcg ccaaaaagta ataaaacggt aagcacttgc actgtttatt tttatcttga
    14041 catatctatg caatattaat taaaagaacc tatatgatag atgaagagtt atctatgtaa
    14101 aattctgaat ctaaaggaat tatattagat ctacaataca gggtaagggt tatgaagtga
    14161 tcttaaaacc ttaagcacaa ggaggcggat atgaaatata tagtaactgc attaatgatt
    14221 gttatttgtt tatgccctct gcaagctatg cgaaaagcgt tagtaatcgg aaatgctaat
    14281 tatgcggaag gcattttaca taattccatt aatgatgcaa cagacatcgc tgctacttta
    14341 aaggacttag gttttaccgt atcgttgcat aaagatctta atcgtgaagg tttttataaa
    14401 gcagtggggg attttgtttc ggcacttgtt tcttcagatg aagcggtctt tttttatagt
    14461 ggccatgcag cacaaattga gggcacaaat tatcttatac ccataaatga acatatcgat
    14521 tctgcaacgc gctgtgccta tctttcctat aatacttcta tgcttttgga ggaattaagc
    14581 agagctgctg ttagtattgt tattcttgat gcctgcagag ataatccttt tgaattttcc
    14641 aggtctttac gaagtggttt agctccaatg aaagctgccg caggaagtca atatgtaatt
    14701 tttgcaaccg aggaaggtaa aactgccgtt gagggtgaaa accgcaacag tccttttaca
    14761 gaatctttat tagccaatat gcgaacacct ggtttaaaaa ttacagactt tgttcaaatt
    14821 gtaagtaacg aagttgctat aaaaacaagg gaaaggcaaa ttccctatag cacaggtatt
    14881 ctcaggcagg atttttattt tgctaaagct gaaccaataa cacctgcccc ccgaatttct
    14941 gataatctcc ctgtagaagc caaaactttt acccctcacg gtgttggctc aattattgta
    15001 aaaacctctt taggaggcga tatctatctt gacggcaatt ttatccagag tttatttgca
    15061 ggaaatcaag ctacttggac agatattcct gttgggattc acgaaattaa attagttaat
    15121 cctcgggaat ccgttatccg taatctgaaa gtagaatata atcaaactgc tgtactcgat
    15181 tttgatacga aggagatagc taaagacaaa atcgaagaaa aacctgtgct gggatctata
    15241 aacacaaatt ctttatcccc ggaaccgcat tttgtaaaag aaccgccttt accaattgta
    15301 aataacgcaa ctaatggaac aggaactttg attattactg ctgcctacga atgcgatgta
    15361 tatcttgggg gacagtttat tgggaaagtg gaagctgggc aaaaatgtaa atttgccaaa
    15421 gagaatgctg gtgtctatac tttggaatta atgcatcgca gtttattctg ccgtagagaa
    15481 attaaaatac ataataaaga aacccttaat cttattctga cgacatcaga ttatattccc
    15541 tatcccgatg ctctactata tgtttctgga ggaacttttc aaatgggaac ctattttatt
    15601 aaccgaagta gtgaagaaca tccccgtcac gaagttcagc tttctccatt tttgatgagt
    15661 aaaacagaaa tagagcaaaa actttggact gaagtaatgg gctataacca ttctgagata
    15721 aagggtggaa atttccctgt aactaatgtt tctttttatg aagcgctgga attttgtaat
    15781 gagttaagcg ttcgcaatgg cttaactccc tgctatataa tagataagaa attacttgat
    15841 cccaataacc tctgcgtttt agatactctt cattatactg tagtttgcaa ttggaaagcc
    15901 aatggctatc gtttaccaac ggaagctgaa tgggaatatg tatctcgttg tggagatagc
    15961 tataacaaag tgcaattctc tggtagcaac aatatttcta aagtttcttg gtattccgac
    16021 aatagcgaag accgcattca ggaagtggca acaaaagaac ctaacgaatg gggattctat
    16081 gacttaagcg gtaatgtttt agaatggtgt tgggattatt ttgatgccta taataaacaa
    16141 aaacaggtta atcctaaggg ttccgaaaaa gggatttacc atattgcccg tggcggaagt
    16201 ttttatagac tagctgatgc aagtacttgc acagcacgtt ttggttttgc ttcccatcat
    16261 aagagtaatg aattgggttt taggattgtg cgtaatgtgc attaattcac tgcttgtaac
    16321 cataatgagt aaaattggag ttcttacttt ttgttgacag gaagttgcag ttatatacat
    16381 aagtataaaa gcaaaattta ctattgaggt gaagacaatg aaaaagctgt atcttatttt
    16441 aattgtgctg gttggcacaa ctcttttagc agctgaacgc ggcttaactg ttaacgaaat
    16501 tccagtttcc gatgaagtta ctactgtttc tactcctgtt cctgcatttt ttaataacga
    16561 atccaaagct aacaagagca aattggaatt tcccaagagc tgggatacac tgcaagaact
    16621 gaatttcaac tatcgtcttc ccgctaaaca acaagttggt atgcaattta tgaatttaac
    16681 gcataatgtt cagccctttg gttaccctta ttccctttcg gaaacggctt ttcaggcaat
    16741 caacaaatcc cctgaatggc tgcaggcaga actttcttct gttttagcac aactggaacc
    16801 ggaaaaacag ttgctctggt caaatttgat tctttccacg caagatcctt atgtggatga
    16861 aattgctttt tgcattgctt attcttcacc tcaatattta aattctactt ttgctctgcc
    16921 ggaactcttt acggaaaatg caaccgctat ttattccctt gccacagagc ttccttatgt
    16981 ggaaattata gatacgggaa attctgcaac aggtggaaat tattattcca caacccgtta
    17041 ctttaaaaag aacaccaatg gagaaatgca acaggttacg gtccctcaag agatatatta
    17101 ttggtatatt gttcatccca agcttaccga tgaaatagcc gcctacatca atcctacggt
    17161 tatagaaaat aattctaacc ataacaataa catagctgct cctcccacag gcaaattttg
    17221 gagaacctat ctctataacc ttcaggaagg tgactatcca gttctaagcg ataccttagt
    17281 tcaatgccaa actcttttta atagggatgg aactggaaac gatgctatcc gcacaattca
    17341 atggtggata aatcaacaga tgagtttcac ttccaataac gaaagaccgc accaaccggt
    17401 tagaattgtc accaaacatt ttggacgctg tggagaatat gctgacttta ctgccgcggt
    17461 agctcgtctt gccttaattc cttgcaccag tatcatttcc atatcaacag atcacacttg
    17521 gaacgaattt tgggatgaga attgggttgc ctgggaaccg gtaaatggct atatagatga
    17581 tcctttggtt tatgaaaacg gctggggcaa ggtctttggc agcgttatcg aaattcgctc
    17641 cgatggttta tttactcctg taacggatag atattccgaa ggactttgcc atattaccat
    17701 tcaggttgtg gactccaatc agcgccctgt ggatggtgcc agagtagttc ttgccatttt
    17761 ggacggcagc aatgtttttg attgcgatca atataccaat aataacggca ttgtaacttt
    17821 tac
//
LOCUS       DAOSTH010000028        17594 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3346_length_17594_cov_8.467815, whole genome shotgun sequence.
ACCESSION   DAOSTH010000028 DAOSTH010000000
VERSION     DAOSTH010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 17594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17594
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3346_length_17594_cov_8.467815"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PLB65_04885"
     CDS             complement(<1..180)
                     /locus_tag="PLB65_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HOQ77898.1"
                     /translation="MEAKLLERIKEQLVRHEGLRLKPYRCTAGRLTIGIGRNLDDCGI
                     SQSEAYVMLINDIMNC"
     gene            483..2060
                     /locus_tag="PLB65_04890"
     CDS             483..2060
                     /locus_tag="PLB65_04890"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOQ77899.1"
                     /translation="MLKVVIFLVFCSLAGSVYSQSPMQLLVSIESPFESDTNSWGGNV
                     KGNGDINGDGYNDIVLAGQPLGSGNGRRDVYIYLGGSLVNTAPDYIIPDPASSTGYYS
                     SFGSSIAYNGDLNGDGYCDLVISEPQYGYDHWGRVLAYYGGPDFDIIPDIEWNGQDYG
                     IITFNTCFGRNINTTGDFNGDGYADLVVNSAHLNEMHFGQINVFYGGPGIDTISDWQY
                     TGEVGEAFGNPLSVGDINGDGFSDLVTFSSIIEGYSNRAIRVFLGGSEFNNQADYSYQ
                     VQPPTEVADLIMDSDFDRDGFDDLIISLEYQNAEFYILFGSSQLDNSLEIIHQSQSVN
                     PRSIYTSEFDNLSYFSYGTPQLESFNFYQFVDHATWALAYVINENYNPNAAQQVSYFL
                     GDINGDGHSEIILSNRTATPVLFEVFTTEYNGNAIDDEIITSPLHLTAYPNPFEKSLS
                     LTYDLNEPGIIKLSVFNIKGQLVARIDEGYKSAGQNSVNWNGMDNIGRTLPSGVYLVK
                     LSISDKHILCKKVTLCY"
     gene            2077..6204
                     /locus_tag="PLB65_04895"
     CDS             2077..6204
                     /locus_tag="PLB65_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ77900.1"
                     /translation="MKHMFFVVAVCILFVPAMGVSYSGSFGVSDIQFSIGERGYDSVR
                     MNGLENIKVVGAPQVPVKLLNFIIPPGMDVDNISIVSNTQILSNAYNLIPVQPQIPTS
                     DEWQPGPFVEPDSTYYTMSVYPPTSAEVLNYGYFDGNARIATIAIYPLQYMPQSKQIV
                     LNSSIGFNLSFKASSQIAVYPTKRTSYLQEKYNALLSNIVENPEDIQMYYSQPPSIGL
                     LPTEADYVIVAPAELISCFNDFVSWKAAKGLHIYTKSIEEIIAQYPNGDQVVPGCIGI
                     DDDAGSIRQYLFDQYSQHGLVYALLVGDDTIFPIRYGYGATDYPSIPADIYFAEFGSK
                     WNVDNDNIYGEEWEFLDRISEIFVGRLLVPSLSGGAAQFISNWFEKIVTYETNPGYGD
                     YSYLTKSFYSVADQMAADLETHNILLSQVNLLEANGFQTTVIEENPPNGPLGTPTGTE
                     VVNEVKNGYGLQSFYGHGNVTRVAVATTNYNTSGGPKYGLFSSEEDEDSDYVNEIGNS
                     FDSFLQDGRYSVNYSMSCDTAAYDEESSFQGGDRNCMARAFTTILDKKGGPVYLGNTR
                     AGLVYGSTVMHKKFLDVLFHTQSVYGQYNCTNVGIPEAASKILYYDKFLWFSHNLFGD
                     PEMPIWTEQPKQIVLSYSTQNNSIHVVNAETNVAIPNAMVHFWNASSSQSELRVTDSN
                     GNAVCSFPYTDVSVTKVNHIPLLKHIVPTGGEVWATNQDLKYDVIVPAGRSLTILDDV
                     NLSMLSKNRNARIIIEDGASLYIDNATITGKATSYDQIKGNAIIVYGDLTIGDNVVFS
                     SVGGLNWDGLYIDNQANSTINNATFNRCNLKKLNGDIVVNGSTFINAEFISSDATVSI
                     NNSNFTGSIDISNSNETSLTGVTVNSLYRNSPAIKIYNTPQLQIENVNVSGAGDGIEM
                     QSCRDYSINNSSFSNNSGNGISIYETNGMYGYAISDCEITDNAGVGVRFYNSIGNLTG
                     CMIANNNKGVTAYRVSNVTIGNTPDNEDSYIGNNELQEILFVDSANLFIDGERNMVVD
                     NAYDPITFDKYLIQCPNLQSSRPMRLNFWGYRNQHDIAIMPPESRFYPECIGPDAGEI
                     GYYLDPVWDPGIPRTIDQDNDAIIFYSAIDAALDENPTLAISLFKQLISEFPGSEFAA
                     ASAKHIYALENDKLALKDYYLSEPNLHYNGEIDKIIDYLTTHCNIKLGNYQEAIAWFE
                     DVISNPPSELDSLMAVIDLGYVYMLMEGDDKASVIAMYPQLKPKSLLEFEANRESILS
                     NLYTSVENPYGNGSNTSSIVAVVPVLERNFPNPFNPSTTLSFSLPSEMVCKLEIYNIR
                     GQKVKTLLNESLQSGRHSIVWDGKNAHGRSVSSGVYFYRLDTPNMTQTSKMLLMK"
     gene            complement(6248..7060)
                     /locus_tag="PLB65_04900"
     CDS             complement(6248..7060)
                     /locus_tag="PLB65_04900"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77901.1"
                     /translation="MLGNAAYTGNSRGFHCMPRNEVMRHMLDENVGVVIGRQGLAVGD
                     NQLWNLVTISQIITDFNLFYQGGGLIAPLYIYPDEHKEDIFTTTERGYNIAPDLLDRF
                     SKLWPQFQPEQLFYYVYAILHSNQYRERFAQYLRMDFPRIPFTEDYELFSKLAEIGKE
                     LADIHLLKSPRLSPPLARYQGSGSNDMVEFIKYDQTSGFVQINPDKHFEGITPELWNY
                     HIGGYQVLHKYLKDRKGNPLADPIHYCRMVTAMALTIDLQERIYKIIGTIIQ"
     gene            complement(7735..8967)
                     /locus_tag="PLB65_04905"
     CDS             complement(7735..8967)
                     /locus_tag="PLB65_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ77902.1"
                     /translation="MDKLQTDSRNKKGIMRSVKSIFGINSSMLGMLIMVILVGMGEKM
                     AERFLPLYLIAMGGTSIIIGLLNGMDNLLSALYSFPGGWLADKIGYKKALNIFNLMAM
                     FGYLIVIIFPSWIAVLVGAVFFISWTAISLPATMSLISSVVPVNRRSMGISMNSIVKR
                     IPMSLGPILGGALILRYGRIQGVRYAFIVAFILAGVALLVQSKFITEQPKKEKTEVIK
                     SSRRFSANLRHLLVSDILVRFCEQIPYAFVVVWVVENNHINELQFGILTTIEMVTAIL
                     IYIPIAYLADRSSRKPFIAITFANFTLFPIVLYFCHNFTSMIFAFIIRGLKEFGEPTR
                     KALIMDLAPEDAKASIFGTYYMIRDVIVSFAAFGGAFLWERNPKINFFTAAAFGLLGT
                     LYFTLMVKPKSVNTIAEA"
     gene            complement(9122..9403)
                     /locus_tag="PLB65_04910"
     CDS             complement(9122..9403)
                     /locus_tag="PLB65_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOQ77903.1"
                     /translation="MSRDELVKKIAASAGISQKVASAALNAVLEGITESLKKGDKVSL
                     VGFGSFSVAHRKARNGINPKTKKPLKIPARNVPVFKAGKNLKEAVKKAK"
     gene            9704..10891
                     /locus_tag="PLB65_04915"
     CDS             9704..10891
                     /locus_tag="PLB65_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ77904.1"
                     /translation="MKCKIMTFIVLTLLVLLSACSGNKKVEMQTEQVPVRNPLVLAHQ
                     AATSGTDLYNKELYNDAIKSFSEAIDLFTEASATASAVDSIAENIEKMKLNIAKSYIE
                     LASESTEMNMYEDALVFYNKALNIYTSIAPTLISEEELNQNIIGLYNNMAIVAKQAGK
                     YENALEYYNQILKLNPKDTDTIYAKFYVLKDNLNDENRAYNVLIEYAELTRDAKAFIN
                     IGDFYADKGNMIEASKYYQKALAIEPNVDVYRRLANYYRITKDWANANVYLLKVVESN
                     PEPSELAQIYIMIGKNYDEMKDKKKMVEYYEKSLSIERDPQLALLLASYYNSQKNYAK
                     VISNANITLSIDPKNVDALMLRGVAYFQTKNYTAAKADLERLKNDPKYGAQATNLLKN
                     IPK"
     gene            10948..11391
                     /gene="umuD"
                     /locus_tag="PLB65_04920"
     CDS             10948..11391
                     /gene="umuD"
                     /locus_tag="PLB65_04920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOQ77905.1"
                     /translation="MKLHSDGSIKAIYTAEQGKKQYRPLMSSKIAAGFPSPAQDYIEG
                     KLDLNEHLITHPASTFFVRVDGYSMQGAGILPDDILIVDRALEAVNNNIVIAIVDGEL
                     TVKRLIIKGNDYYLMPENDEFEPIQIQEGMDFVIWGVVTFVIHKV"
     gene            11396..14647
                     /locus_tag="PLB65_04925"
     CDS             11396..14647
                     /locus_tag="PLB65_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77906.1"
                     /translation="MASIKPCIFLLLFLSGITYLFAQSLYEQHNLVLEPNQQKYTLTN
                     SPIIKNSVCVWADTLLLKEGIDYIVDHKKAELILLYEPEAPVLKVEYLLIPSSLTKPL
                     QLWEPRLYSDSLLSNIKKRSNPVFSSDARLNIQGAKTFSISFSDDSAFDLKQSLYVNL
                     SGELANNVYINAQLSDSQSKLSPEGDSKELSSLDNVFIRIYGPKYELAMGDLEIKYTG
                     TRYMEYYSKFEGINAWVKGKHYAQTAFSAGGGKNASVKINIIEGKQGPYYLKANDTQS
                     NFLVLAGSEEIFIDGNKLERGLDYSIDYSEGSVMFKRLVTASNTVIARFQYSDEFYPQ
                     SSYLNSTKVQLSEHLSFSHHFIWQQDDKKNPLIYEFSTADIDSLQAAGDNQIWGEGVL
                     TVEAGTGNYKQLLNSQNEYYYEYAPGDSLACYNIIFSFVGSGNGDYEEYSLGKYRYIG
                     QGLGSWLPQKRLIPPEKKGNLDMAFAYSNEGLNAGIEVLGTVNDQNTLSIKDDNDNNA
                     GLIYAYLEIPYSLYSLKLDYEQRSEQTYLFGKYRNPNEEYDFASIETADSLAQHESNI
                     QLSRQQPNWNSSLLFRYRKIYDLYSLQAFRFISSSSGKGFLPAINIRSTISKQDYQKD
                     DDLNGTMQYHQGDISWFIYNIRLHFETLYNLLDTSTFGNSYLKYSPGITIGKASSFLT
                     QLSHSSDITKLNDNDHWNIISETQTYALKQIVNLNSQRLDLDVTHRVVKQPYSTSNPE
                     SNYDLITFRSSNSFLKNAIGLYSNYQVNQTEFYPKIRELQYVGHNLGYYDSTGVSVSN
                     GDYDWVYITSPTGSLSSEINCLLNIYLKPGNISSNNLLKCLQSDTSVNLIEHNSKRNN
                     WQSYFFLPGSVYNDESTIYGSQNIQEVLWIDLVRNKITSNLQFSMERTLDKRYQTPDR
                     TYSFLQLAQFDIKGYSEYNTRLQFSKENNQDTRYLNESEILSINAMIQKNLNLQSNIQ
                     AEIIYSSESGGRQDGSENYTLQSISLSPTMKSVFMQKYRLTAKLGVTYNKLSGSDYFT
                     FLPQKRAGWIPSASISGVYRINSFSVINLDYRYSDYPKQRDTQELKLEFKAEI"
     gene            14651..15436
                     /locus_tag="PLB65_04930"
     CDS             14651..15436
                     /locus_tag="PLB65_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOQ77907.1"
                     /translation="MHNIILTPLDYLIVLPFIFLAGLIDSIAGGGGLISLPAYWSVGI
                     PPHLALGTNKFSSCCGTLFSTANYFKARMIDIPVAVISAGFALIGSWLGASSALKVSP
                     AVLNYLLIFLIPAVAVFSLLKRNMGMQSTAQLLAKSYRLIISAITGLGIGFYDGFFGP
                     GTGTFLLLIYTSLLHYDFVTGNGNTKVVNLASNLAAVITFAFAANIYYPLAIPGAICG
                     IVGNVVGSKLVIARGNKLIKKVFILSLVLLFTRILYNLAFSKT"
     gene            15541..16626
                     /locus_tag="PLB65_04935"
     CDS             15541..16626
                     /locus_tag="PLB65_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ77908.1"
                     /translation="MKKVLFLLTALLLVLTACSTARQKLSPQGNVNLKTADVYYSQEN
                     VDQAEIYYLKVLEDNPDHVIALRRLGDISLFKAENFTAREVEFYEDAFEYYSKAISVT
                     ENFPELTNQDRIDLRDMRKRKERAWARIYMAAEKEKEAGNTQKAKEIFELAHKLEPDR
                     PEPMIQLKNIYLVDIKDEVKAEQILKQLLQKDPDKLEYLLEMGTFYYNKGNYAEAVKY
                     FDHARVQNPTNVDNLMNISACYYELKDYEKAMIATKTALEIEPNNLDLLENARSIADQ
                     MNDLDQSIEYLKRILDIRPNEDTYSILAAHLMQKQDWQQLIKYAEEWYNWDTTNKIAV
                     EYIIWAAQQSGNRLLATKYSEIKNKMP"
     gene            16756..>17594
                     /locus_tag="PLB65_04940"
     CDS             16756..>17594
                     /locus_tag="PLB65_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOQ77909.1"
                     /translation="MSNQTKAYIFAGCSIIAWSTISTAFKLSLQFLTPIGLLFFASCS
                     AVLFLNITQSIRNPSFYRVKGLLARILKNLKASFLSGLLNPFVYYLMLFEAYSRLRAQ
                     EAQALNYTWAIVLSIFSIWLLKEKFRLLDFIALLISLFGVWIISTKGQITSLRFDDAL
                     GSFLAVSTSIIWAFYWILNIKDERETITKLSFNFLVGFMLIALFVVCTGASLFTDEAK
                     YGYGILGGIYVGIFEMGLTFILWNRALELTDNTAAVSNLIFFTPFVSLLFISSILKES
                     IHYA"
BASE COUNT         5612 a         3044 c         3409 g         5529 t
ORIGIN      
        1 gcagttcatg atgtcattga tcagcatgac gtaggcttcg gattgggata tcccacagtc
       61 atcgagattg cgaccgatgc cgatagtcaa tctgcctgcg gtgcagcggt aaggttttaa
      121 ccgcaaacct tcatgtctaa ctaattgttc tttgatgcgt tccagtagct tggcttccat
      181 cgtatctcct tgcgagtcgt attggatact ctatccggag caaggaagcc actaccccgt
      241 attaatacaa atacggatgc ataacgatgc gaaagaattt ggttattgtt tttttatttg
      301 ctttttcttg gctttgggtt attttatttt taattgagag ttgaggtctg ccatgtggta
      361 ttggttttag ttaaataata cccagagtta tgtttaaaaa tatcagggat tagactatta
      421 taggatgtat gaatgtatcc ttttggcact aagaaacaag aacaaagtat attggagtga
      481 ttatgctgaa agtagttata tttttggtct tttgcagttt ggcagggtcc gtttattcgc
      541 aatccccaat gcaattattg gtttctattg aatctccctt tgaaagtgat acaaatagct
      601 ggggtgggaa tgttaaaggc aacggagata taaatggcga tggatacaat gatattgtgt
      661 tggctggtca gcctctcgga agtggaaatg gacgtagaga tgtatacatc tatctgggag
      721 gaagcttagt aaacacagcc cccgattaca ttattcctga tcccgcatct tcgacaggat
      781 actatagttc tttcggttca tcaatagcat ataatggtga tctgaatgga gatggatatt
      841 gcgatttagt aatctctgaa cctcaatacg gttatgatca ttggggaagg gtactggcat
      901 attatggtgg tccggatttt gatattatcc ccgatattga atggaatgga caagattatg
      961 gcataattac tttcaatact tgctttggaa gaaatattaa tacaactgga gattttaacg
     1021 gagatggtta tgctgattta gttgttaatt ctgctcacct gaatgagatg cattttggac
     1081 aaataaacgt cttctacgga ggccctggga tagatactat tagtgattgg caatatactg
     1141 gagaagtagg ggaagcattt ggcaatccat tgtcagtagg ggacattaat ggagatggtt
     1201 tcagtgacct ggttacattt tcatcgatta ttgaaggtta tagtaatcgg gcaatcagag
     1261 tttttcttgg tggtagcgaa ttcaataatc aagcagatta ttcctatcaa gtacaaccac
     1321 caacagaagt ggctgatttg atcatggatt cggattttga tagggacggt tttgacgatt
     1381 taatcatctc attggaatat cagaacgctg aattctacat tctatttgga tcgagtcaat
     1441 tggataattc cttagaaatt attcatcaat cacaatcagt aaatcccaga tccatctata
     1501 cttctgaatt cgataaccta agctatttca gctatggaac tcctcaattg gagagcttca
     1561 atttctatca gtttgtagat cacgctactt gggcactggc ttatgtaatc aacgaaaact
     1621 acaatcccaa tgcagctcag caagtatcat attttcttgg ggatatcaat ggggatggac
     1681 atagtgagat tatactatct aaccgcaccg caaccccagt tttatttgag gtgtttacca
     1741 ctgagtataa tggtaatgca atagatgatg aaattattac ttctccttta catctcactg
     1801 cctatccaaa tccgtttgaa aaaagcttgt cactgacgta tgacttaaat gagcctggaa
     1861 taataaaact ttcagttttc aatattaagg ggcaattagt tgccaggatt gatgaagggt
     1921 acaagagtgc agggcaaaac tctgttaact ggaatggaat ggataatatt ggtcgcacac
     1981 taccttcagg agtctatcta gttaaactat caatttctga taaacatatt ttatgcaaaa
     2041 aagtaacgtt gtgttactaa ttatcaagga ggaaagatga aacacatgtt ttttgttgtc
     2101 gcagtctgca tattgtttgt tccagcaatg ggggtaagct acagtgggag ttttggggtg
     2161 tcagatattc aattctcaat cggagaaaga gggtatgatt cagttaggat gaatggatta
     2221 gaaaacatta aagtagtagg tgctcctcag gtgccagtaa agctattaaa ctttattatc
     2281 ccacctggaa tggatgttga taatatatct attgtttcca atactcaaat actttcaaat
     2341 gcgtataatt taatccctgt gcaacctcag atacccactt cagacgaatg gcaaccggga
     2401 ccattcgtag aaccagatag tacttactac acaatgtcag tttacccacc gacatccgca
     2461 gaagttctga attatgggta cttcgatgga aatgcacgaa tagctactat agcaatttat
     2521 cctcttcagt atatgccaca atcgaagcag atagtattga actcttcaat aggttttaac
     2581 ctctcgttca aagcttcctc acagattgca gtatatccta cgaaaagaac aagttatcta
     2641 caagaaaaat acaatgcact cctcagcaat atcgttgaga atccagaaga cattcagatg
     2701 tattattcac aaccgccatc aattggcttg cttccgacgg aagctgatta tgtgattgtt
     2761 gctccagcag aattgatatc ctgctttaac gactttgtgt catggaaagc tgctaaaggt
     2821 cttcacatct atactaagtc tatagaagaa attattgctc agtaccctaa cggggatcag
     2881 gtggttcctg gatgtatagg aatagatgat gatgctggat caattagaca gtatttgttt
     2941 gatcaatact ctcaacatgg tcttgtatat gctttactgg ttggtgatga tacgattttc
     3001 cccattagat atggatatgg ggcaaccgac tatccatcta tcccagccga tatttacttt
     3061 gctgagtttg gaagcaaatg gaatgtcgat aacgataata tttatggaga agaatgggaa
     3121 tttctagatc gtataagcga aatatttgtt ggacgtttat tggtgccatc attatctggt
     3181 ggagcagcgc aatttattag taattggttt gaaaaaatag tcacttatga aactaatccc
     3241 ggctatggcg attattcata tttgacaaag tcattctata gtgtagcaga ccagatggct
     3301 gcggacttag aaacacataa tatacttctt tcacaagtta acctacttga agccaatgga
     3361 ttccaaacaa cagtaattga agagaacccg cctaatggtc ctctcggaac tccaacggga
     3421 actgaagtgg ttaatgaagt aaaaaatggt tatggactac aatcattcta tgggcatggg
     3481 aatgtaacaa gggttgctgt tgcaactact aactacaata cttcaggggg gcctaagtat
     3541 gggttgttta gctcagaaga agatgaagac tcagattatg taaacgagat aggcaattca
     3601 tttgacagtt tcttacagga tggtagatac tcggtaaact actcaatgtc ttgtgataca
     3661 gcagcctatg acgaagaatc aagcttccaa ggcggtgata ggaattgtat ggctagggct
     3721 ttcacaacaa ttttagacaa gaaaggtggc cctgtttatc ttggtaatac tagagcaggc
     3781 cttgtctacg gatcaacagt aatgcataag aaatttcttg atgtgttatt tcatacacaa
     3841 tctgtatatg ggcagtataa ttgcactaat gttggcattc ccgaagcagc atctaagata
     3901 ctatattatg acaagtttct gtggttttca cacaatcttt tcggagatcc tgagatgcca
     3961 atttggactg aacaacccaa gcagattgtg ctgagttata gcacccaaaa caactcaata
     4021 catgttgtga atgctgagac caatgtagca atccctaatg ctatggttca tttctggaat
     4081 gcaagttcct cacaatcaga acttcgagta actgactcaa atggcaatgc tgtttgtagc
     4141 ttcccttata ctgatgtttc tgtaaccaag gtaaaccata tccccttgtt gaagcatatc
     4201 gtgccaacag gtggggaagt atgggcgact aatcaggacc ttaaatatga tgtcatagtt
     4261 cctgctggta gaagcttgac tattttggat gatgttaatc tcagcatgct ttctaaaaat
     4321 cgaaatgcga gaataataat tgaagatgga gcgtcattat acattgataa tgccacaatc
     4381 accggcaaag caacttccta tgatcagata aaaggcaatg ctatcattgt ctatggtgat
     4441 ttgactattg gtgataatgt tgtgttttcg tcagtcggtg ggttaaactg ggatggattg
     4501 tatatcgaca atcaagcaaa tagcactatt aacaacgcaa ctttcaatcg gtgtaacctt
     4561 aagaaattaa acggagacat tgtagttaat ggatctactt tcattaatgc ggagtttata
     4621 agcagcgatg ctaccgtgtc tatcaacaat tccaatttca ctggctctat tgatatttcg
     4681 aactcaaacg aaacgagcct cactggggtt actgttaata gtctctacag gaactcacct
     4741 gcaatcaaaa tctataatac tcctcagctt caaattgaga acgtaaatgt aagtggagct
     4801 ggcgatggca tagagatgca aagttgcagg gattattcaa ttaacaacag ctcgttctct
     4861 aataactccg gtaatggaat cagtatttat gaaacaaatg gaatgtacgg ctacgctatc
     4921 tcggactgtg agattacaga taatgctgga gttggagtaa ggttctacaa ttctatcgga
     4981 aatctgacag gatgcatgat tgccaacaac aacaaaggcg tcactgcata tagagtgagt
     5041 aatgtaacga tcggcaatac ccctgataac gaagactcat acattggcaa taatgagtta
     5101 caagaaattc tatttgtaga ttctgctaat ctgtttatcg acggagagcg gaatatggtc
     5161 gtagacaacg cttatgaccc catcactttt gataaatact tgattcagtg tccaaacttg
     5221 caaagcagta gaccaatgcg gctgaacttc tggggatata ggaaccaaca tgatatagcg
     5281 attatgccac ctgagagtag attttatcct gaatgcattg gacctgatgc aggtgagatt
     5341 gggtattatc ttgatcccgt atgggatcct ggcattccaa gaacgattga ccaagacaac
     5401 gacgcaatca tattttactc cgcaattgat gcagctctgg acgagaaccc aacacttgcg
     5461 atttccttgt ttaagcagtt aatcagtgaa tttccgggca gtgagtttgc tgctgcaagt
     5521 gcaaagcata tctatgccct tgagaatgat aagcttgctt tgaaggatta ctacttatct
     5581 gaacctaatc tgcattataa tggagaaatt gacaaaatca tagattatct aactacacat
     5641 tgcaatatta agctgggcaa ttatcaagag gctatcgctt ggtttgagga tgtgataagc
     5701 aatcccccaa gtgagttaga ttctttaatg gcagttattg atttgggtta cgtttacatg
     5761 ctcatggagg gagatgacaa ggcatccgtt attgccatgt acccccagtt aaagccaaaa
     5821 tcactacttg agtttgaagc aaatcgggaa tcaatactct caaatctata cacttctgtt
     5881 gaaaatccct acgggaatgg gagcaataca tcttcgatag tagcagttgt gccggttttg
     5941 gaaaggaact ttccaaaccc attcaaccca tcaacaacac tttcattctc cttgccatct
     6001 gaaatggtgt gtaaacttga gatttacaat attcgtggac agaaagtcaa aactctgttg
     6061 aatgaaagtc ttcagtcggg cagacactcg attgtttggg acggcaaaaa tgctcatggc
     6121 agatcggtct cttcgggagt atacttctac agattagata ctccaaatat gacccaaaca
     6181 tcaaaaatgt tgttgatgaa atagtatcaa attacaccat tagaagcccg gggcaacccg
     6241 ggcttttcta ttggataatg gttccaataa ttttatatat ccgttcctga agatcgattg
     6301 tcaatgccat tgcagttacc atcctgcaat agtggatggg atcggccaaa ggattgcctt
     6361 tgcggtcttt gaggtattta tgcagcacct gataacctcc tatgtgataa ttccagagct
     6421 ccggtgttat gccctcaaag tgtttatccg ggttgatctg cacaaaaccg ctggtctgat
     6481 cgtacttgat gaattccacc atgtcgttgg aaccactacc ctgataccgt gctaagggag
     6541 ggcttaatcg tggactcttg agtaaatgga tgtcggcaag ctctttgcct atttctgcca
     6601 acttactgaa gagttcataa tcctctgtga agggaatacg ggggaaatcc atccttagat
     6661 attgcgcaaa gcgttcacgg tattgattgc tgtgcaggat cgcatagaca taatagaata
     6721 gttgctcagg ctggaactga ggccagagct tgctgaatct atctaaaaga tcaggagcga
     6781 tattgtatcc ccgctctgtg gtggtaaaga tgtcttcttt gtgctcgtca gggtagatgt
     6841 aaagaggggc tatcaaaccg cctccctgat aaaagagatt gaaatctgta atgatctgag
     6901 agatggttac cagattccac agttggttgt cacctacagc aagaccctgc ctaccaataa
     6961 ccacacctac attctcgtcc agcatatgcc tcatgacctc gttgcgcggc atacagtgga
     7021 atcctctgct atttccagtg taggctgcgt ttccaagtat cggagggcat tctatgtagg
     7081 gtctgtaagc tgtattctat gtgagatgga gctgtatttc tattaatgaa gattcgtgta
     7141 gattcataat tagattgagt tgataggcat cgctatcttt aatatacatc tctattgtta
     7201 gactttttgt tcatttcttc ctccttaatt ttagtccacc atttttaata tggctcaaac
     7261 aaacaaccca tcctcctctc ctcctcctcc cgtcctcaac ccgtcgttaa ggaggagtga
     7321 agcttgggtg gatgacaagt tgtatattgt taacttgtaa gagaaagcaa ggtttagatt
     7381 aaaactatct ttatatattg ttaatagtta tggtgttagt aaatctcaag ggtttcaaat
     7441 aatcgatctt aatgtgattt ttttatcttg atgaataatc cgctatatat tgatagtggt
     7501 agtatctcaa agattgatta aaaatcggga aaaccaattg gatatgcgca acaatttcta
     7561 aaataataca taggccgcta taacacaatc taaagccaat cctatttatt tccgcaactc
     7621 attaacctaa aaggcttctg tgatatatga tatggatttg aaggtttggg tgtaaactta
     7681 tatacaccta tcccagctct gaaataactc tcaataaaaa ctaaatagta attgctaagc
     7741 ctctgctatg gtattcaccg attttggctt taccatcaaa gtaaagtata gtgtacctaa
     7801 tagaccgaaa gccgcagctg tgaaaaaatt aatctttggg tttcgttccc acaaaaaagc
     7861 tcctccaaaa gctgcaaaag aaacaatcac atcccgaatc atataataag taccaaaaat
     7921 cgatgctttg gcatcttctg gagcaagatc cataattaag gcttttcttg taggttcgcc
     7981 aaattctttc aagcccctaa taataaaggc aaaaatcata gaggtgaaat tatggcagaa
     8041 gtataatact atagggaaaa gtgtgaaatt ggcaaaagta atagcgataa aaggtttgcg
     8101 actacttcga tcagccaaat aggcaattgg gatataaatt aaaatagcgg ttaccatttc
     8161 gatagtggtt aagattccaa attgtaattc attgatatga ttattttcta ctacccaaac
     8221 aactacaaat gcataaggaa tctgttcgca aaaacggact aaaatatcgg aaacaagcaa
     8281 atgacgcaag ttagcagaaa accgacggga acttttaata acttcagttt tctccttttt
     8341 cggctgttca gtgataaatt tactttgaac caataaagct acacctgcta aaataaaggc
     8401 aacgataaaa gcataacgga ctccctgaat tcttccatag cgcaaaataa gagcacctcc
     8461 caaaattgga cctaatgaca taggaatgcg ttttacaatg gaattcatag aaatccccat
     8521 agaacgacga ttcacaggaa ctacagaaga aatcagactc atcgtggcag gtagggaaat
     8581 agctgtccag gaaataaaaa agaccgctcc cactaaaaca gctatccagg aagggaaaat
     8641 gatcacaata agatatccaa acatagccat cagattgaat atgtttagag cttttttgta
     8701 acctatttta tcagccaacc atcctcccgg aaatgaatat aaggcactta ataaattatc
     8761 cattccattt aaaagaccga taataatgga agtgcctccc atagctatta aatataaagg
     8821 gagaaaacgt tctgccattt tttctcccat tcctaccaga ataaccatta tcagcattcc
     8881 caacatacta ctgttaattc cgaagataga ttttacgcta cgcattatcc ctttcttgtt
     8941 tctactgtct gtttgtagtt tgtccattta gttgtccctt ttcaatttag taggttattg
     9001 aaaaaatatg tgctcaaaga taaaatatag aagaaagcaa aaataccaag tgtaatttta
     9061 taggttaata caaaaggcag aaagacaaaa ggcttcctgc ccattttgta aagtaactga
     9121 attatttggc ttttttaaca gcctctttaa ggtttttacc tgctttgaag accggaacat
     9181 tgcgagccgg aatcttcaaa ggttttttgg ttttagggtt aataccattg cgtgctttac
     9241 gatgggctac gctgaatgaa ccaaaaccaa ccaatgaaac tttatcccct tttttcaggg
     9301 attcggtaat gccctcgaga actgcgttga gggctgcact ggcaactttt tgtgaaatac
     9361 cagcactagc tgcgattttt tttaccaatt cgtctctgct cattttacct ccttatttgg
     9421 ttctcttgta aagagtgaga caccttacaa tcgttattaa agtgcactat gatatttttg
     9481 tcaataaaaa taattacatt tatcgtattt gcaacgattt tatgtggttt gactcctgca
     9541 ctctaagaag ataattatat atgatataag gagttacaag aacgacacta aatgcaataa
     9601 ttttcttgat atttatctat tttatagctt tttacttttt taagttgaca aaaatcagag
     9661 agctaagtag agaggatatc aaataataat ggaggattta ccgatgaaat gtaaaataat
     9721 gactttcata gttctcactc ttttagtact acttagtgct tgcagtggga acaaaaaagt
     9781 ggagatgcaa actgaacaag ttcctgtgcg taatccttta gtattagctc atcaagctgc
     9841 tacttcagga acagatttat ataacaaaga gctatataat gatgcaataa aatcattttc
     9901 agaagcaatt gatttattta cagaagcatc tgcaactgct tctgctgtgg attcaatagc
     9961 tgaaaatatt gaaaaaatga aattgaacat tgcaaaatcc tatattgagc ttgcttcaga
    10021 aagcacagaa atgaatatgt atgaggatgc gttggttttt tataataaag ctcttaacat
    10081 ctatacttct atagctccta ctttaatttc ggaggaagaa ttaaatcaga atattattgg
    10141 tctttacaac aatatggcta ttgtagctaa acaagctggt aaatatgaaa atgcgttgga
    10201 atattataat cagattctaa aactgaatcc taaagatact gatactatct atgctaagtt
    10261 ttatgttttg aaagataatt tgaacgatga aaaccgtgct tataatgtcc ttatcgagta
    10321 tgcagaacta actagggatg ctaaagcatt catcaatatt ggggattttt acgctgataa
    10381 agggaatatg attgaagcaa gtaaatatta tcaaaaagct ttagctattg aacctaatgt
    10441 tgatgtttac cgtcgtttgg caaattatta tcgtatcaca aaagattggg caaatgctaa
    10501 tgtctatctg ctgaaagttg tagaatccaa tccggaacca tctgagctgg cacagattta
    10561 tattatgatc gggaaaaact atgacgaaat gaaggacaag aaaaaaatgg ttgaatatta
    10621 tgaaaagtcc ttaagtatcg aaagagatcc gcagcttgct ctattactgg caagttacta
    10681 caattctcag aaaaattatg ccaaggttat tagcaatgcc aatattacgc taagcataga
    10741 tcctaaaaat gtagatgctt taatgctgag gggagttgct tattttcaaa ctaagaatta
    10801 tacagcagcc aaagctgatt tggaacgcct taaaaacgat cctaaatatg gtgctcaggc
    10861 aacaaatcta cttaagaaca ttcctaaata ataaactagc attcaaggac gggttaatca
    10921 atatacccgt cctgctcttt ataaattatg aaactacata gtgatggttc gataaaagct
    10981 atttacacag ctgagcaagg gaaaaaacaa tatcgtcctt taatgagttc gaaaattgca
    11041 gctggctttc catctccagc tcaggattat attgaaggaa aacttgatct taacgaacac
    11101 cttataaccc atcctgcctc cacttttttt gttagagtag atggatattc tatgcaagga
    11161 gcaggaatat taccggatga tatactaatc gtggatagag cattagaagc ggtgaataat
    11221 aacatagtaa tagcaattgt agatggagaa cttacggtta aacgtttgat aataaagggg
    11281 aatgattact atttaatgcc cgaaaatgat gaatttgaac cgattcagat tcaagagggt
    11341 atggattttg tgatttgggg tgttgtaacc ttcgtaatcc ataaagtata atcagttggc
    11401 atctattaag ccctgtattt ttttattact ttttctttca gggatcactt atctctttgc
    11461 tcaatctctg tatgaacaac ataatcttgt gctcgagcca aaccaacaga aatacacact
    11521 taccaattca cctattatca aaaacagcgt ttgtgtttgg gctgataccc tgttgctgaa
    11581 agagggaatt gattatatag tagatcataa aaaagcggaa ttgattttat tgtatgagcc
    11641 tgaagctcct gttttaaaag tagagtattt actaattcca tcttctttaa caaaaccttt
    11701 acaattatgg gaaccacgcc tttatagtga tagtttatta tctaatataa aaaaaagaag
    11761 caatcctgta tttagcagtg atgctcgtct gaatattcaa ggggctaaaa ccttctctat
    11821 aagcttttct gatgattcgg cttttgattt gaaacaatca ctttatgtaa acctctctgg
    11881 agaattggca aataatgttt atatcaatgc tcagctttcg gatagccagt ccaaactatc
    11941 tccagaaggc gattcgaaag agcttagttc attggataat gtatttatca gaatttatgg
    12001 accgaaatat gaactggcaa tgggtgatct ggaaataaaa tatacaggaa ctcgctatat
    12061 ggagtattat agtaaatttg aaggtatcaa tgcctgggtg aaaggaaaac attatgccca
    12121 aactgcattt tctgctggag gaggcaaaaa tgcgtctgtt aaaatcaata tcatagaagg
    12181 aaagcaagga ccatactacc taaaagcaaa tgatactcaa tccaattttc tagttttggc
    12241 tgggagtgaa gagatcttta ttgatggtaa caaattagag cggggattag attatagtat
    12301 tgactatagt gaaggtagtg taatgtttaa acgcttggta acggcttcca atacagttat
    12361 agctcgattt cagtattctg atgaatttta tccgcaaagt agttatctta attctacgaa
    12421 agtgcaactt tcagaacatc tttcattttc tcatcatttt atctggcagc aggatgataa
    12481 aaaaaatcct ctaatctatg aattcagtac tgctgatata gattctttac aggcagctgg
    12541 agataaccag atttggggtg aaggagtttt gacagtcgag gcaggaacag gtaattataa
    12601 acagttacta aattctcaaa acgaatatta ttacgaatat gcaccggggg attctcttgc
    12661 ttgttacaat atcatcttta gttttgtggg tagcggaaat ggagattatg aagaatattc
    12721 tcttgggaaa tatcgttaca taggacaagg attggggtct tggcttccgc agaaacgatt
    12781 aattccacct gaaaaaaaag ggaatttaga tatggcattt gcctattcca atgaaggtct
    12841 taatgcagga atagaagtat tgggaactgt gaatgatcag aatactttat caataaagga
    12901 tgataacgat aataatgcag gtctgattta tgcttacttg gagattccat actcactata
    12961 ttctttaaaa ttggattatg aacagcgaag tgaacaaaca tacctctttg gaaaataccg
    13021 gaatccgaac gaggaatatg atttcgcttc catagaaaca gcggattctt tagcccagca
    13081 cgaaagcaat attcaattaa gccgtcagca acctaattgg aatagttcat tactatttcg
    13141 atatcgaaag atatatgatc tttattcgtt acaggcattt cgttttattt cttcatcttc
    13201 aggaaagggt tttttgcctg ctataaatat aaggagtacg atttccaagc aagattatca
    13261 aaaggatgac gatttaaatg gaactatgca ataccatcag ggagatatat cctggttcat
    13321 atataatatc cgtctgcatt ttgaaactct gtataacctg ttagatacaa gtacatttgg
    13381 taacagttat ttgaaatata gtcctggaat tacgattggt aaggcatcct cttttcttac
    13441 tcaactatct catagctctg atataacgaa actgaatgat aatgatcatt ggaacataat
    13501 ttctgaaact caaacttatg ctttaaagca gattgtaaac ctaaattcac aacgccttga
    13561 tttagatgtt actcatcgtg tagtgaaaca gccatattct acttctaatc ccgaatccaa
    13621 ttatgatcta atcacttttc gttccagcaa cagctttctc aaaaatgcta ttggactata
    13681 tagcaactat caagtaaatc aaactgagtt ttatcctaaa atccgtgaat tgcaatatgt
    13741 tggacataat ctaggctatt atgacagcac aggagtaagc gttagcaatg gtgattatga
    13801 ttgggtttat ataacaagcc ccacagggag tttatcttct gagataaact gtttgcttaa
    13861 tatatatcta aaaccaggaa atatatcctc gaacaatttg ttgaaatgtc ttcagagtga
    13921 tacttcagta aatttgattg aacacaattc taagcgaaat aactggcaga gttatttctt
    13981 cttgccgggt agcgtgtata atgacgaatc cacaatctat ggaagtcaaa acatacagga
    14041 agttctctgg atagatttag taaggaataa aataacatcg aacttgcaat tctctatgga
    14101 acggactctc gataaacgtt atcaaacacc tgatagaaca tatagcttct tacaattggc
    14161 acagtttgat attaagggtt atagtgaata taacacccgt ctacagttta gcaaagaaaa
    14221 caatcaggat acacgctatt taaatgaatc ggaaatatta agcattaatg ctatgattca
    14281 aaagaacttg aatctgcaaa gtaatattca agcagaaatt atctatagtt cagaatctgg
    14341 aggaagacaa gatggcagtg aaaattatac actccagtcg ataagtttat caccaactat
    14401 gaaaagtgtt tttatgcaaa aataccgttt aactgccaaa ttgggagtaa cctacaataa
    14461 actttcagga agcgattatt ttacttttct tccccaaaaa agagctggtt ggattccttc
    14521 tgcatcaata agtggagttt atcggataaa tagtttcagc gttattaatc ttgattatcg
    14581 ttattcggat tatccaaaac aaagagacac tcaggaacta aaattggaat tcaaggcgga
    14641 aatataaact atgcataata ttattttaac ccctcttgat tatttaattg ttttgccctt
    14701 tatcttcttg gctggtttaa ttgattctat agcaggtgga ggaggtttaa tttctctacc
    14761 cgcatattgg agtgtaggaa ttccacctca tttagcttta ggaaccaata aattttcttc
    14821 ctgttgcgga acactttttt ccactgccaa ttattttaaa gcaagaatga tagatatccc
    14881 tgtagctgtg ataagtgctg gatttgcttt aattggttcc tggttgggtg cttcaagtgc
    14941 attgaaagtt tcgcctgccg ttttaaatta tctattgatt tttttgatac ctgcagtagc
    15001 agttttttct ttactgaaaa gaaatatggg aatgcaaagc acagcgcaat tattagctaa
    15061 aagctatcga ctaattatat cagcaataac tggactcgga atcggatttt atgatggttt
    15121 cttcggtcct ggaactggaa catttttact ccttatttac acttccttgc tgcattacga
    15181 ttttgtaact ggcaatggta atactaaagt tgtgaacttg gcttctaacc ttgcagcagt
    15241 gataactttt gcctttgctg ctaacatata ttatccactt gccataccag gtgcaatttg
    15301 tggtatagta ggaaatgttg ttggttctaa attagttatt gcaagaggaa acaaacttat
    15361 taagaaggtt tttattctct cgttagtgct cttatttaca agaatacttt ataatttagc
    15421 atttagtaaa acctgatact tggaattaaa aaattaaggt gttagcgaaa attacttgac
    15481 tgaatagcaa cttcatattc tttgattttt taagattaaa atatcttagg agaatactgg
    15541 atgaaaaagg tgttattctt attaactgca ctattattag tgcttactgc ttgttctaca
    15601 gcacgccaaa agcttagtcc ccaaggtaat gtgaatttaa aaactgcaga tgtttactat
    15661 tctcaagaga atgtggatca agcagaaatt tactatttga aagtgcttga ggataatccc
    15721 gatcatgtaa ttgcattgcg tcgtttggga gatatcagtt tattcaaagc ggaaaatttt
    15781 actgctcgcg aagtagaatt ttatgaagat gcctttgaat attattcaaa agccatcagt
    15841 gtaactgaaa atttccccga gctaacaaat caggaccgta ttgacttacg ggatatgaga
    15901 aaaagaaaag aaagagcttg ggctcgtatt tatatggctg ctgaaaaaga aaaagaagca
    15961 gggaacacac aaaaagctaa agaaattttt gaattagccc ataaactgga accagatcgt
    16021 cccgagccaa tgattcaact taaaaatatc tacttagttg acatcaaaga tgaggttaag
    16081 gcagaacaaa tattaaaaca attactacag aaagatcctg ataagcttga atatttgtta
    16141 gaaatgggaa ctttttatta caataaaggc aattatgctg aggcagttaa atattttgat
    16201 cacgcgagag tacaaaatcc tactaatgtt gataacttga tgaatatatc tgcttgttat
    16261 tatgaattga aggattatga aaaggcgatg attgctacaa aaaccgcttt agaaattgaa
    16321 cctaataatc ttgacttgct tgaaaatgct cgttctattg cagaccagat gaatgatctt
    16381 gaccaaagta ttgaatatct taaacgtata ttggatatcc gtcctaatga agatacatac
    16441 agtatattgg ccgctcactt aatgcaaaaa caagattggc agcaacttat taaatatgca
    16501 gaggaatggt ataattggga tacaactaat aaaatagctg tagagtatat tatctgggct
    16561 gctcagcaat ccggaaatcg acttcttgca actaaatatt ccgaaatcaa gaataaaatg
    16621 ccttaatctt ttttaagagc cattaggctt tcagttagga gtcctcactg ctaaaagaga
    16681 taagaactta taaaaataat agggcagtga gaactccttt cttatttttc agccagttcc
    16741 caaggactct gtttaatgtc aaatcaaact aaagcatata tatttgctgg ttgttctata
    16801 attgcttggt ctacgataag cacagctttt aaactatctt tacaatttct aacccctatt
    16861 ggtttattgt tctttgcttc ttgtagtgct gtactttttt taaatataac ccagagcatc
    16921 aggaatcctt ctttctatcg agtaaaagga ttattagctc gcattttgaa gaatctgaaa
    16981 gcatcttttc tttctggttt gttaaatcca tttgtttatt atttaatgct ttttgaggca
    17041 tattcaaggt tgcgggcaca agaagcgcaa gccttaaatt acacttgggc tattgtttta
    17101 tcaatattca gtatttggtt gctaaaggaa aagtttcgtc ttcttgattt tattgcgtta
    17161 ttaatcagct tatttggagt ttggataatt tctacgaagg gtcaaattac ttcacttaga
    17221 tttgatgatg cattaggttc ttttttagca gtttctactt ctataatttg ggcgttttat
    17281 tggattctta atattaaaga tgagcgagag acaattacca aactgagttt taattttctg
    17341 gttggattta tgttaattgc tctttttgtt gtttgtacgg gagcttctct ttttacggat
    17401 gaagcaaaat atgggtatgg aattttaggt ggtatttatg ttggcatttt tgaaatgggt
    17461 ttaaccttta tactttggaa cagggcattg gaattaacag ataatactgc agcagtttcc
    17521 aatctgattt tctttactcc ttttgtatct ttgcttttca tcagttctat tttgaaggag
    17581 agcattcatt atgc
//
LOCUS       DAOSTH010000029        17425 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3393_length_17425_cov_14.198733, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000029 DAOSTH010000000
VERSION     DAOSTH010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 17425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17425
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3393_length_17425_cov_14.198733"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            514..1440
                     /gene="lepB"
                     /locus_tag="PLB65_04945"
     CDS             514..1440
                     /gene="lepB"
                     /locus_tag="PLB65_04945"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOQ77910.1"
                     /translation="MRDKISAKKDKTKLDALPYTVTPPPEGKKFHKRKPGLQDWVEAI
                     LFAFVVAMIIRNYTFENFLIPSSSMEKTLLVGDYLVANKIKYFFSDPKQGEIVTFRYP
                     KIEEGTPEHKDYKDDFIKIFPPIYINKKQSFSKFPLTAFHISYYARRNIVKRVIGMPG
                     DTVEIKDKIVYVNGKEYTKGFENYGVAIPSPPASPERIEYFTYPENSFDYVSMNPWYE
                     PYRIKPEGDSTAVRKIFNRDWFGPIKVPENSYFVMGDNRDVSEDSRYWGFLERKHITG
                     TPWLIFFSRGIEFNKLYDEPHIRWNRIFRHPS"
     gene            1410..2534
                     /gene="hemW"
                     /locus_tag="PLB65_04950"
     CDS             1410..2534
                     /gene="hemW"
                     /locus_tag="PLB65_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOQ77911.1"
                     /translation="MEQDFQASQLGLYIHIPFCLTKCGYCSFFTLPFRQSSLYEYVGY
                     IEKEKVLYRYQLKRPLATIYFGGGTPSLLSAEQIMAILEGLNILPDAEITLEINPLQI
                     TEQYLKALKTTPVNRLSIGMQSMNNSELAWLNRHHNMEQIIDKIALCRKENFNNISLD
                     LIYGLPNSSVQTVQQTVDAYLKLQPEHISCYLLTLDEDCPLADEIDKLPNEDILAEQY
                     EYLCSHLKQAGYIHYEISNFAIPGFESKHNLRYWKSEDYLALGVSASGWIYPMRYQNP
                     VDINAYYENVKNRVIFPQKEELSNQRIIQDYLMMGLRLREGINLQEFNERFQIPLMEI
                     YREQINKLIKIKMLTLQEGNLALTEQALFVSNRVIGDLIL"
     gene            2531..3268
                     /locus_tag="PLB65_04955"
     CDS             2531..3268
                     /locus_tag="PLB65_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HOQ77912.1"
                     /translation="MNLMEWRILLDKLINDSDFAHKSYFAGGCVRDFLLNPDSSITSD
                     IDICVEIFEGGIALAKYLQNYLPESDIIIHQSFGTASLRYKNYKLEFVATRKEKYYPG
                     SRYPQVQFGNLLDDVLRRDFTINALLMSITTGEILDLSCKGLSDLKQGIIRTVQDPDK
                     SFSEDPLRMLRALQFALRFNFQIEDKTLKAMQKNTPALKILSKRAIQEELNKIPESKR
                     QEAIAFIDKMNWKPYLKHKSETQNEQC"
     gene            complement(4059..5486)
                     /locus_tag="PLB65_04960"
     CDS             complement(4059..5486)
                     /locus_tag="PLB65_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOQ77913.1"
                     /translation="MHAKIVKSGVRWTLATTILRRGISLILFIFLAKWLTQTDFGIYR
                     SFSAILALLTYLTHFGLDYLFLISKSKERINFLALLQTGLFVSAVITILLIFFGKTIG
                     TYYNSAELGRILTYGGGLIFVESLRRIARVYAQKRFQFKELAIAETLNVFIYSLLCLG
                     IIYFWRKAWVYILLFYIGNLGELIYLCIKLPPLPATLQKQVFSFKWLKHSCAYFKRNF
                     QFLGIVSTINLLQSYSINAPVLFLGTMVEPALMGVYFFASQLIAIPIGMLNTSLSQVF
                     FPVLAQSETAASVSGIRHYTAIVLKIGIPALIFYALILQYLVPLIWGDKWLSALPLVL
                     YLVLFYGTSMLHNPISGIPYICRKPQWELLWNIVTLGLRIIALFLGMKVSFAFAILLF
                     CIVSAFMHLAFYYLSLTLLKANLWQITIDLISNLPVLILIAFGCLYLWKFSVIFPLLT
                     FVGYGLYLFLAEKDTLKEVLSLAIK"
     gene            complement(5496..6512)
                     /gene="ruvB"
                     /locus_tag="PLB65_04965"
     CDS             complement(5496..6512)
                     /gene="ruvB"
                     /locus_tag="PLB65_04965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOQ77914.1"
                     /translation="MLERINDPDQLIEDIELDRALRPRSLKEFIGQTHIKELLDISIK
                     AARLRGESLDHILLYGPPGLGKTTLAGIIAYELGVNITTSSGPVIEKPSDLAGILTNL
                     QGHSVLFIDEIHRLSHVIEEYIYPAMEDFEMEIILDSGPNSRTLKIPLEQFTLIGATT
                     RAGLLTPPLRDRFGIVLRLDYYDQESIAHIIRRSAKLLNIPADNDGINEIARRSRGTP
                     RIANRLLRRVRDYAQIKGNGIITLNIALAALQMLQVDNAGLDEMDKRILTTIIENYNG
                     GPVGIKTIATAVGEDSGTIEEIFEPYLVQQGFLERSPQGRKVTFKAYRHLGLTPLTEQ
                     TEIF"
     gene            complement(6516..6974)
                     /locus_tag="PLB65_04970"
     CDS             complement(6516..6974)
                     /locus_tag="PLB65_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77915.1"
                     /translation="MKRFTLFSILLLLCLTLSAITIPRVYVQKLTLENDKNPLITREQ
                     DKSANEYILKAWINTRPDEIVSTETHPIHTIAVKQVGDGNKFPFMVITSLQLGNFKSQ
                     WQAGEVIYMEITHIKTGQKKTWTQPIPEGTNLIKMLDKPVVIPPYTKKKK"
     gene            complement(6984..7706)
                     /locus_tag="PLB65_04975"
     CDS             complement(6984..7706)
                     /locus_tag="PLB65_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HOQ77916.1"
                     /translation="MKTLIIIPTYNEIENIERLLEQVLSKSDSIEVLVIDDNSPDGTA
                     LRVKFMQSTNPRIHLLQRPGKMGLGSAYVTGFKYALEQGYDFIMEMDADFSHNPDDIP
                     RLLEAAQKYDLVIGSRYCEGVNIIHWPFKRLLISYFASKYVRVITGMPIKDPTGGYKC
                     FRRKVLESIDLDKILSDGYAFQIEMNFRAWVKGFHLKEIPIIFTERINGVSKMSRKIV
                     WEAAWMVWRLQIMKVLGLINNK"
     gene            complement(7703..8458)
                     /locus_tag="PLB65_04980"
     CDS             complement(7703..8458)
                     /locus_tag="PLB65_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HOQ77917.1"
                     /translation="MNQYIDNSAKIGKNVTFGYNVVIMEGVQIGDDCLIGHNVVIHPD
                     TKIGNSCRIDDGTIIGKKPLSSPRSIFSVSTNLKAAEIGDFCQIGSNAVIYCQCEIGN
                     HNLIADLATIRENVSIGDLNIIGRNVAIENYVKIGNRNKFETNCYITAYSEIGNYCFV
                     APCVATSNDNYMGRDIERYKHFRGITMIDGSRIGLNATILPGKTINSDGTVAAGAVVT
                     KDVPVETVVAGNPAKPMRTVPETQLMKHNLDIQ"
     gene            8529..9425
                     /locus_tag="PLB65_04985"
     CDS             8529..9425
                     /locus_tag="PLB65_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HOQ77918.1"
                     /translation="MEKYIDDFCNYLALEGKSEKTISAYKLDLEQFYTYIQRFFENGI
                     VTLPEITVLNIRDFLRYLNEKPDCNRSLARKSAALNSFFKFCKKSGFISNNPMDKIKR
                     PKYEVPLPKCFTEEEVRTLLSIPDTSNRYGVRNKAILETLYSSGLRLSELAGIRLQDI
                     DLKRGLVRVTGKGNKQRIVPLGSYAREAIYNYLKIRPKFAQENSPDLLFLTKSGKAFD
                     TKQLDIILKRYFELVAKAKGYSPHSLRHSFATHLLSRGADLRAIQELLGHSLLSTTEV
                     YTHISMEDIKEAYKKGHPRYKE"
     gene            9992..10471
                     /gene="nuoE"
                     /locus_tag="PLB65_04990"
     CDS             9992..10471
                     /gene="nuoE"
                     /locus_tag="PLB65_04990"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOQ77919.1"
                     /translation="MNNVLKRLDKIITTYQGKKGSLIPLLQETQNELGFLSRETMEYL
                     AEKMQIPAAEIFGVATFYSMFRLEPQGKHLIRVCKGTACHVSDADGIKKAVVDALQLS
                     EGENTTSDRQFTVMEVACLGCCSLAPVIMIDDRTYGKLVPEAIPAILHQYISETSNE"
     gene            10464..12233
                     /locus_tag="PLB65_04995"
     CDS             10464..12233
                     /locus_tag="PLB65_04995"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOQ77920.1"
                     /translation="MNNIIVKVGLASCGVAAGAKEVYNALKQYLKENDKPVTLKKTAC
                     IGMCFEEPIVQFEGSELGSIHIGKARPETIISILEDYIAGKAPAENIILAEKLPSRHN
                     DLLANQQRIVLRNCGIIDPECIEDYEARDGYKALQKALSMDRDAIIDEIKKSGLRGRG
                     GAGFPTGVKWQLCAKEVSAKKYVICNADEGDPGAFMDRSTLEGDPHSVIEGMIIGAYA
                     IGANEGYIYCRAEYPMALEHLKTAIAAAEKKNYLGKNILGSSFSFDLHIKEGAGAFVC
                     GEETALMQSIEGKRGMPTLRPPYPAAKGLWGKPTNINNVETWANVPWIIMHSANEYRK
                     YGTDKSPGTKVFALAGKIAGSGLIEVPMGIPLKDIIYKVGGGMKTSKPFKAVQMGGPS
                     GGCIPAEKIDTIVDYDSITATGAIMGSGGMVVMDTGTCMVDVAKFFLNFTQNESCGKC
                     TFCRIGTKRMLEILTRITEGKGELQDLNNLEELAENIIKGSLCGLGQTAPDPVLTTLR
                     YFKDEYLAHIVDKRCPAGVCTALITYKIDPNKCIGCTLCARKCPVSCISGKVKQVHTI
                     DLEKCIKCGACFDACKFEAVVKE"
     gene            12245..15550
                     /locus_tag="PLB65_05000"
     CDS             12245..15550
                     /locus_tag="PLB65_05000"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOQ77921.1"
                     /translation="MIEVILNGKTVKAEAGISILELCQQHNIDIPTLCNDEELNPYGS
                     CWVCLVEVKGRKGFVTSCGTTISEGMEIITNSEEITRARKMALELLISNHYADCVAPC
                     TIACPDQVDIQTYISLIANGKYHEAVKVIKEKLPMPLSIGRICPAFCEKECRRQIVDE
                     SIAIRQLKRYAADVDNEDVWNYVPEKLSTKNKKIAIIGAGPSGLTCGYFLSNWGYEVI
                     IYEAAPQAGGWLRYGIPEFRLPKDILDAEIELMCTNGMQIEYNKQLGKDIYLQNLADS
                     YDAVYLAIGAQKAVPMPVKGSDLDGCFLGVDFLKAHALGNSPNLGLKVAIVGGGNTAV
                     DCARTAIRKGCEVAVIYRRTKAEMPAEASEIVACENEGVKFFYLANPIEYIGKGGHLT
                     SVKIEKMRLGEPDKSGRKRPEPTGEFFELPFDSIIAAISQVPEVDIFTEEKNYVADKV
                     LPISRWQTAIVDETTMFTGLENVFAGGDFRRGAATAIEAIADGRIAAKAIDNYLATGE
                     IVAELIPFDSKKAKSVKDISPAEYSIFEPKKRKEMPELPLGEAKNSFKEVELGFLEED
                     AINEAKRCLECGCFVNETCSLRKYCTDYLVNPAHFLGGINKHPIDYSHPFIVRDANKC
                     INCGRCIRTCAEIQGPAVLGFIYRGFGTMVAPEFGDSLTKTSCLSCGKCIDVCPVGAL
                     TERNMHYKFNPHPKDISLQNCALCGVGCVIQTETQAGTITKITTSQKEPGFNGKNLCF
                     KGRFGWQGLYDKDRLNVPLLKQNGYFKEISWQEAASLIAEKIALCNSQRFEISPHISL
                     EEMLMLQRVADKYETKLCANPAFAYFSDSFLSLKPESEPYKILENYDEYVIYGELNQV
                     LSSLIRLQQRKGKKLVLVNYPESPYRRFADANYANLQEVQATQKTLFVYNQNRITEQE
                     AYNLWCFASKIGTENILVSTDYRNHLGFLAMKPDFSEKVADFVLGWGVYPALANQAKF
                     SIAIMNFQDETAPVDLLIPAPSFLEIEGTSLSDLAQITKSANPAKSIVINELMRLMYK
                     LNWIHPNSAEIPFWNYEAEKLLISLQNYAPNKFEPSFIKPEDLNKGIKVIPAQAEKYI
                     AALFELRQKPTSF"
     gene            complement(15841..17190)
                     /locus_tag="PLB65_05005"
     CDS             complement(15841..17190)
                     /locus_tag="PLB65_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4080 domain-containing protein"
                     /protein_id="HOQ77922.1"
                     /translation="MKKILMTAINSSWSQSNLALYYLREVIADLPYRVFMQEWTLKDQ
                     LLDVLYGIYVQNADVICFSAYIWNRLYLQELIPELVKLLPEAIIVIGGPEAGNLTYLQ
                     RDRFYFIQGAGEGKFRYLAENNFCLKEEELTQAKHIPLKDIPFPYRQSDAEFLKGKLL
                     YYETFRGCPFACIYCLSAKDKRFELRYHPENQKDMQCLKKELEALIALEPRTIKFVDR
                     SFNINKRLAHYIWQFFIEQNCPCDVHFEIYPDLIEEEDIALLEKAPENRIRLEIGIQT
                     ISAEIAAKSGRKSNWEKAKWVLRELKKRTKLRVHTDLLAGLPGENYFSIINGLNEVCA
                     TLPDEVQLGILKILPDTSMQEIAIALNYKWLHQPPYQCLSSNALSFEEMHQLESFAKL
                     LNLYWNKGEHKKEWQEMLQNRSAAEILTALQQKHKELGYELHSLSKAKRDAVMAEIV"
BASE COUNT         5570 a         3175 c         3349 g         5331 t
ORIGIN      
        1 tggtcatctt gatctccttc tcggttaccc gactgttgat ttatgggtgc tactaaacac
       61 ccctcgcaca ggtggtcaag tcctttccga tcaatatgat aagagagatc gatgtctaaa
      121 taaacaatcg gaatgaatgt gagcctacct taatcgcaag ccatgcaatg ccatgcgaaa
      181 tctagttgaa gaaatcgtga cattccccct cgatccagcc ctgtttcatc agtttttatg
      241 ctagaaaccc ataacctgtt ccgcagattt cacttaagaa aaccatttga aaaagtcgtg
      301 aaattccatt tgaaaaaatc gtgatatcgc aggccagcaa aaacgctaat ttgaaaaatt
      361 ggtgatacga tttgaaaaaa taagtgagaa cgcataaaaa gagggataat ttttaccctt
      421 gccacctaag ctctcctaag cttttacttg acacaaaccc tgttcttaaa gctttgtaat
      481 ttaaataggt ataaatgata aggatattta ctgatgagag ataaaataag cgcaaaaaaa
      541 gacaaaacta aattagacgc cctaccctat actgttactc cccctcctga agggaaaaaa
      601 ttccataagc gtaaacctgg tttgcaagat tgggtggagg caatcctttt tgcttttgta
      661 gtggcaatga tcattcgtaa ttatactttc gagaatttct tgatcccctc ttcttctatg
      721 gaaaagacac ttttagtggg tgattatttg gtagccaata agattaagta ctttttcagt
      781 gatcccaagc aaggtgaaat cgtcacattc cgctatccca aaattgaaga aggcacacct
      841 gaacacaagg attataagga tgattttatc aagatctttc cgcctattta tataaacaag
      901 aaacagagct tttccaaatt tcccttaact gcttttcata taagctatta tgcccgtagg
      961 aacattgtta aacgagtgat cggaatgcct ggcgacacag ttgaaataaa agataaaatt
     1021 gtttatgtga acggcaaaga atatacaaaa ggttttgaaa attatggagt ggctattccc
     1081 agtccacctg cttctccaga acgaattgaa tattttacct atccagagaa cagctttgat
     1141 tatgtttcaa tgaatccctg gtatgaacct tatagaatta agcccgaagg tgattccacc
     1201 gctgttcgta aaatattcaa tcgcgattgg tttggcccaa taaaggttcc cgaaaacagt
     1261 tattttgtaa tgggcgacaa tcgtgatgtt agtgaagata gtcgttattg gggtttttta
     1321 gaacgaaaac atataaccgg gactccctgg ttgattttct tcagcagggg aattgagttt
     1381 aataaacttt atgatgaacc ccatattcga tggaacagga ttttcaggca tcccagttag
     1441 ggctttatat ccatattcct ttttgcctta caaaatgcgg gtattgcagt tttttcactc
     1501 tgccttttcg acaaagttca ttatatgaat atgttggcta tatagaaaaa gagaaagtac
     1561 tatatcgcta tcagttaaaa agacctttag ccacaatcta ttttggcggg ggaactcctt
     1621 cccttttaag tgcagaacag attatggcta ttttagaagg cctaaatatt ttacctgatg
     1681 ccgaaatcac tctggaaatt aatcctctgc aaattacaga acaatattta aaggcactga
     1741 aaacaacacc tgtaaatcgc ttgtctatag ggatgcaaag tatgaataat tcagaacttg
     1801 cctggctgaa tagacatcat aatatggaac aaataataga taaaatagct ctatgccgta
     1861 aagaaaactt caataatatt tccttagacc taatttacgg attgccaaat agttcagttc
     1921 aaaccgtaca gcaaacagtt gatgcctatc tgaaacttca accggaacat atttcctgct
     1981 atcttttaac ccttgatgaa gattgtcccc tggccgatga aatagataaa ctgcccaacg
     2041 aagatattct ggctgaacaa tatgaatatc tttgtagcca tttgaaacaa gcagggtata
     2101 ttcactatga aatatccaat tttgctatac caggctttga atcaaaacat aacttaagat
     2161 actggaagag tgaggattat ttggctttgg gagtttctgc ctcaggatgg atttacccta
     2221 tgcgttatca aaatcctgta gatatcaatg cctattatga aaatgtgaaa aatagagtta
     2281 ttttccccca aaaggaagaa ctctctaacc aaagaattat tcaggactat ttgatgatgg
     2341 gattgcgttt gcgagaaggg attaacctac aagaatttaa tgagcggttt cagattcccc
     2401 ttatggaaat atatcgtgag cagataaata agctgattaa gataaaaatg cttactctgc
     2461 aagaaggaaa cttagcttta acagaacaag ccctttttgt ttccaatcgc gtaattggag
     2521 atttaatatt atgaatttga tggaatggag aatacttctt gataaattga ttaatgatag
     2581 cgattttgcc cataaaagtt actttgccgg gggctgtgtg cgcgattttc ttttaaatcc
     2641 cgatagttct atcacaagcg atattgatat ctgtgtggaa atttttgaag gaggaattgc
     2701 cctggccaaa tatctacaga actatctgcc ggaaagtgat attattatcc atcaaagttt
     2761 tggaactgct tccttgcgct ataaaaacta taagctggaa tttgtggcta cccgaaaaga
     2821 gaaatattat cctggcagtc gttatcccca agtacaattt ggaaacttac tggatgatgt
     2881 cttaaggagg gattttacta ttaatgcatt acttatgtct attaccacag gtgaaatttt
     2941 ggatctttcc tgtaaaggac ttagcgattt gaaacaagga attattcgca cagtacaaga
     3001 cccggataaa agtttttcag aagacccttt aaggatgcta agagcgctgc aatttgcttt
     3061 acgctttaat tttcaaatag aagataaaac actaaaagca atgcaaaaaa atactcctgc
     3121 cctgaaaatc ctttctaaaa gagcaataca agaagaacta aataaaatcc ctgaaagcaa
     3181 acgacaggaa gcaattgcgt tcatagataa aatgaattgg aaaccctatc taaagcataa
     3241 aagtgaaacc cagaatgaac aatgttagct atattaatat agcatatcag cacaccagaa
     3301 gcgagtggta aagacagaaa gtacaccctc cctttatttt ttattcctat taagattcat
     3361 ataaatatca attacaattg gaaggattaa agtaacagca taaaatacta cgaaaactcc
     3421 cctcttgaaa ctgcattata cttactgtaa caatcaattt gataataaaa tcccgatatt
     3481 tttgtttcct actttccctg ctgggagcca ttaatctatc gtttatctac cctttatcaa
     3541 gcgttaatta ttaacgcttg attaaggctt gattaacact tgattaacgc tttcacctaa
     3601 gggcaaagag cgaagcttga atacaatatg cagtgtatat taaagtggta aatacctgat
     3661 gacagatata tgtaatagga tagtacttaa atatccaccc ttcattttaa tgtataagga
     3721 aaaaagagct taccattttt tgctgggcaa ataaaccgaa gtttgtcaac agaccttgtg
     3781 cttacctgtt tttagaatga ttttattcgg cggttcagtt actgaatatg gctataaact
     3841 atgtggcttc cttatgcgga tttatactca gaggaaaatc tctgatgaat tgcagaagta
     3901 gaatattact ttttccttag taatttaact tattaaaaag gggggtattg ttttctttac
     3961 gccccatcag caagccagaa gtcatatttt gtgtttataa taaatcttga tatcctataa
     4021 tccttgtttc taaaattata atttgcataa tcttagggct attttatagc cagagaaagc
     4081 acttctttta aagtatcttt ttccgccaaa aatagataca atccataacc cacaaaagtt
     4141 aaaagcggaa atatcacaga aaacttccat agataaaggc agccaaaggc aattaaaatc
     4201 aataccggca aattagatat aaggtcaatt gttatttgcc acaaatttgc ttttaacaga
     4261 gttaaagaca aataataaaa agccagatgc ataaatgcac tcacaatgca aaataataaa
     4321 atggcaaaag cgaaactaac tttcattccc aaaaacagag ctataatacg caaacctaaa
     4381 gtaacaatat tccaaagcaa ttcccattga ggttttctac aaatataggg aatgccacta
     4441 attggattat gcaacatact tgttccataa aaaaggacta aatagagaac caaaggcaat
     4501 gccgagagcc atttatcacc ccatataaga ggaaccagat attgcaaaat aagcgcataa
     4561 aaaattagtg caggtatccc tattttcaga acaatagcag tgtaatgcct tatgccagaa
     4621 acacttgctg cagtctcact ctgagctaga actggaaaaa atacctgcga aagagaagta
     4681 ttaagcatcc ctataggaat tgcaattagt tgcgatgcaa aaaaataaac tcccatcaaa
     4741 gctggttcta ccatagttcc taaaaaaagg acaggagcat tgatggaata agactgtaac
     4801 agatttatcg tacttactat tcccaaaaat tgaaagttac gcttaaaata ggcacaagag
     4861 tgttttagcc acttaaaact aaaaacctgt ttctgtaaag ttgctggtaa aggaggtagc
     4921 ttgatgcata gataaataag ttcacctaaa ttgccgatat aaaataaaag gatataaacc
     4981 caggcctttc gccaaaaata gataataccc aagcagagca aagaatagat gaaaacatta
     5041 agtgtttccg ctattgccaa ctctttgaac tgaaatcttt tttgagcata aacgcgagct
     5101 atccttctta gggattccac aaaaattaag cccccgccat aagtgagaat tctgccaagt
     5161 tcagcactgt tataataagt tccgatagtt tttccaaaaa agattagcag aatggtaata
     5221 acagctgaaa caaaaagtcc tgtttgcaga agagccagga agtttatgcg ttctttgctc
     5281 ttggaaatta ggaacagata atcaagccca aaatgagtta gataagtaag caacgccaaa
     5341 attgctgaaa agctacgata gataccgaaa tcagtttgcg ttaaccattt agccagaaag
     5401 atgaaaagaa tcaaactgat tcctctgcgt aaaatagtgg ttgcaagagt ccaacgaact
     5461 ccacttttaa ctatttttgc gtgcattttt ctcttttaga aaatctctgt ctgttcagta
     5521 aggggagtta aacctaaatg gcggtatgcc ttaaaagtaa cttttctacc ttgagggctg
     5581 cgttccagaa agccctgttg cactaaatag ggttcaaaga tttcttcgat agttccggaa
     5641 tcttctccaa cggcagtagc aattgtttta atacctacag ggcctccatt atagttttcg
     5701 ataatagtag ttaagatgcg tttatccatt tcatccaaac cggcattatc cacttgcaac
     5761 atttgtaaag cagccaaagc aatatttaga gtaataattc cgttgccttt tatttgagca
     5821 taatcacgca cacgtctaag taaacgattg gcaattctgg gagtgcccct actgcgtctg
     5881 gcaatttcat taatgccatc attatcagct ggaatattca atagttttgc actacgacga
     5941 ataatatgtg ctatggattc ctgatcgtaa taatccaaac gaagcacaat tccaaatcta
     6001 tctcgaagag ggggagttaa taaaccagct ctggtagtgg ctccaattaa agtaaattgt
     6061 tccaatggta tttttagagt gcgggaattg ggaccgctat ccagaattat ttccatctca
     6121 aaatcttcca tagcaggata gatatattct tcaatcacat gggacaggcg atgaatttca
     6181 tctataaaaa ggactgaatg cccttgtaaa ttagttaaaa taccagccaa atcagaaggt
     6241 ttttcaataa ccggtccgct ggaggtagtg atatttaccc ctaattcata agcaataatg
     6301 ccagccaggg tagtttttcc taaaccggga ggtccatata ataaaatatg atcaagggat
     6361 tcaccgcgta gtctggcagc tttgatacta atatctaaaa gttctttaat atgtgtttgt
     6421 ccgatgaatt cttttaaaga gcgcggacgt aaagctctat ccagttctat atcttcaatc
     6481 aattgatcgg ggtcgtttat tctttccagc atagtttatt ttttcttttt agtatagggt
     6541 gggataacaa caggtttatc cagcatttta attaagtttg tgccttcggg gatgggctgg
     6601 gtccaggtct ttttttgtcc tgtttttata tgtgttattt ccatatagat aacttctccg
     6661 gcttgccatt ggcttttaaa attaccaagt tgcaaactgg taatcaccat aaaaggaaac
     6721 ttatttccat ctccaacttg tttaacagca atagtatgaa tcggatgagt ttcagtactg
     6781 acaatttcat ccgggcgggt attaatccag gccttaagga tatattcatt agcactttta
     6841 tcttgttcac gagttatcaa agggttttta tcattttcga gagttagttt ttgcacataa
     6901 actcggggga tagtaattgc agaaagggtt aaacaaagca gtagcaatat actgaacaga
     6961 gtaaatctct tcatcataac accctattta ttatttataa gacccagaac tttcataatt
     7021 tgtaaacgcc aaaccatcca ggcagcttcc caaacgattt ttcggctcat ttttgatacg
     7081 ccgttgatgc gttcggtaaa gataattggt atttctttta gatggaaacc cttcacccaa
     7141 gctcggaaat tcatttctat ctggaaggca tagccgtcag ataggatttt gtccaaatct
     7201 atactttcca gaactttacg ccgaaaacac ttataccctc cggtaggatc tttaattggc
     7261 attccagtta ttacgcgcac atatttactg gcgaagtagc taattaataa tcgtttgaaa
     7321 ggccagtgaa taatatttac cccttcgcaa tagcgactac caataactaa atcatatttt
     7381 tgggctgctt ccaacaagcg cggtatgtca tctggattat gagaaaaatc agcatccatt
     7441 tccattataa aatcgtaacc ttgttccaat gcatatttga aaccggttac ataagcagaa
     7501 ccgagaccca ttttccctgg acgctgaagt aaatgtatcc gcggatttgt gctttgcata
     7561 aatttcactc ttaaagctgt gccatcagga gaattatcat ctatcactaa tacttcaatt
     7621 gagtcacttt tagaaagcac ctgttccagg aggcgttcaa tattttcaat ttcgttgtaa
     7681 gtagggataa ttattagtgt tttcattgta tatccagatt atgtttcatt aattgggttt
     7741 caggtactgt gcgcataggt tttgctggat ttccagcaac aacagtttcc acaggaacat
     7801 ctttggtaac aactgctcca gcagctacag ttccatcgct gtttattgtt tttccgggaa
     7861 ggattgtagc attaagacca atccttgaac catcgatcat tgtaattcct ctaaagtgtt
     7921 tatagcgttc tatatcgcgc cccatataat tatcgttact tgttgccacg caaggagcta
     7981 caaaacaata atttccaatt tcagaatagg cagtgatata gcaattagtt tcgaacttat
     8041 ttcggtttcc gatttttaca tagttttcga tagctacatt tctgccgata atattcaaat
     8101 cacctatact aacattttca cggatagtag ccagatcagc aattaaatta tggttaccta
     8161 tttcacattg acaatagata acagcattgc tgccaatttg gcaaaaatct ccaatttcgg
     8221 cagctttaag attggtggac acactaaaaa tactgcgtgg agaagagagt ggttttttcc
     8281 caataatcgt tccatcatca attctacagg aattaccgat ttttgtatcc ggatgaataa
     8341 caacattatg tcctattaag caatcatctc caatttgaac tccttccatt ataactacat
     8401 tataaccgaa agttacattt ttcccaattt tggcggaatt atctatatat tggttcataa
     8461 atcctcccct ttttacttta ataccatttt ttactcgaca agttttaagg caagtaaaaa
     8521 atggccttat ggaaaagtat atagatgatt tttgcaacta tctggcttta gaaggtaaat
     8581 ccgaaaagac gattagtgcc tataaattgg atttagagca gttttacacc tatattcagc
     8641 gtttttttga aaatggaata gttactttgc cggaaataac tgttctgaat attcgcgatt
     8701 ttttgcgcta tttgaatgaa aagccagatt gcaatcgttc tctagcgcgt aaaagtgcag
     8761 ctctaaattc ttttttcaag ttttgcaaaa aaagcggctt tatttcaaat aacccgatgg
     8821 acaaaatcaa acgccccaaa tatgaagtcc ctctgcccaa atgctttacc gaagaagaag
     8881 taagaactct gctttcaatt ccagatacca gcaatcggta tggtgttaga aataaggcaa
     8941 ttttagaaac cttatattct tcaggtctgc gtctttctga acttgctggc atccgtttac
     9001 aggatataga tttaaaaaga ggcctggttc gggttacagg aaaaggtaat aaacagcgaa
     9061 ttgtccccct gggttcctat gccagagaag ccatatataa ctatttaaag attcgcccaa
     9121 agttcgccca ggaaaatagc cccgatttac tatttttaac taaaagcggc aaagcgttcg
     9181 atacaaagca gttggatatc attctaaaac gatattttga actggtagct aaggctaagg
     9241 gctattcccc gcattcttta agacatagtt tcgccacaca tttactttcc agaggtgccg
     9301 atttacgagc tattcaagaa ctgttggggc attcacttct ctctacaaca gaagtatata
     9361 cccatatatc aatggaagat atcaaggaag catataaaaa aggccaccct cgttataaag
     9421 aataaactat atttccagtt gggaatttga ggtttgtaaa aaataagctc cctctttccg
     9481 ctcattatag ttgaggttgt ctgctacaat ttttatgtct tttcttgggg ctgggattat
     9541 tatagtagcc attaatgtta taatttcata taaatgccga atattagctg tgtaatatgt
     9601 ctgtaggaat gcaagcataa taaacacttg ctctgtagta aatcctctca cgattcccta
     9661 gtctgactag agatctgttc gctaattatt agcattgtta tcgattataa agcaggtaag
     9721 gattgataat gctaaacagc aaacctggaa taaaataaca tcgatgccta aaagagttac
     9781 agtaattctt cttatcttct ccacaaaatg aataaagaaa taattatctt gatgatagga
     9841 tatagcgtaa taaacaatcc tgtgcttact ttagattatt tgtatacctt ttttgtttga
     9901 catatcctgc cccatcaaaa tatgggttgc tacaaaacat ggtttacctt ggagtaatgt
     9961 ctgatacata tggagaaata gaggtttgga tatgaataat gtcttaaagc gtttagataa
    10021 aattataact acctatcaag ggaaaaaagg ttctctgata cctttgttac aagagacaca
    10081 gaatgaactg ggttttttat ccagagaaac aatggaatat ttagcagaaa aaatgcaaat
    10141 acctgctgcg gagatttttg gagtagcaac attttactct atgtttcgtt tggaaccaca
    10201 gggtaaacac ttaatccgcg tttgtaaagg gactgcgtgc catgtttccg atgcagatgg
    10261 aatcaaaaaa gcagtagtgg atgcgttgca gctttcggaa ggtgaaaaca caacttccga
    10321 cagacaattt acagtaatgg aagttgcctg cttaggatgt tgttccttgg cacccgtaat
    10381 tatgatcgat gatagaactt atggcaaact ggttccggaa gcaattcctg ctattttaca
    10441 tcaatatata agcgagacat ctaatgaata atataatcgt aaaagtaggt ttggcaagtt
    10501 gtggtgtagc tgctggggca aaagaagtat ataatgcact aaaacaatat cttaaagaaa
    10561 atgataaacc tgttacatta aagaagactg cttgcattgg tatgtgtttt gaagaaccga
    10621 tcgtacagtt tgaaggttct gaacttggct ccattcatat tggaaaagct cgaccagaaa
    10681 caattatcag tattttggaa gattatatag caggaaaagc tcctgctgag aatatcattt
    10741 tagcagaaaa gctaccgagt agacataatg atcttttggc taatcagcaa cgcatcgttt
    10801 tacggaattg tgggataata gacccggaat gtattgagga ttatgaagcc cgagatggtt
    10861 ataaagcatt acaaaaagca ctaagtatgg atagagacgc catcattgac gaaatcaaaa
    10921 aatcgggttt aagaggacgc ggtggagctg gtttccctac aggagtaaaa tggcaacttt
    10981 gtgctaaaga agtatccgct aaaaaatatg ttatatgcaa tgctgatgaa ggtgatccag
    11041 gtgcttttat ggatcgtagt actctggaag gcgatcctca ttccgtgatt gaaggtatga
    11101 taattggtgc ttatgctatt ggtgctaatg aaggttatat atactgccga gcagaatatc
    11161 caatggcttt ggaacattta aaaactgcaa ttgccgctgc agaaaaaaag aactacctgg
    11221 gaaaaaatat attgggcagt tctttcagtt ttgatttgca tatcaaggaa ggtgccggtg
    11281 cttttgtttg tggagaagaa actgctttaa tgcaatccat tgaaggtaaa agaggtatgc
    11341 ctactttgcg tcccccatat cctgctgcaa aaggactttg gggaaagcct accaatatta
    11401 ataatgtaga aacctgggca aatgtcccct ggataattat gcacagtgcc aatgaatata
    11461 gaaaatatgg aacagataaa tcacccggaa ctaaagtatt tgccttagca ggtaaaattg
    11521 ctggtagtgg attaatagaa gtccctatgg ggatccctct taaagatata atctataagg
    11581 ttgggggagg aatgaaaact tccaaacctt tcaaagctgt gcaaatggga ggtccatccg
    11641 ggggttgtat tcctgccgaa aaaattgata ccatagtaga ttatgattct atcactgcca
    11701 caggggcaat tatgggttcc gggggaatgg tagttatgga tactggaact tgtatggttg
    11761 atgtagccaa gttttttcta aatttcactc aaaatgaatc ctgtgggaaa tgtacttttt
    11821 gccgtattgg aaccaaacga atgttagaaa tcttaactcg tatcacggaa ggcaaaggag
    11881 aattgcaaga cctaaacaac ctggaagaat tggcagaaaa tataatcaag ggttcacttt
    11941 gcggtttggg acaaacagct cctgatcctg ttctaaccac cttgcgttat tttaaggatg
    12001 aatatttagc tcatattgta gataaacgct gtcccgcagg tgtttgtact gctttaataa
    12061 cctataaaat tgatcccaat aaatgtatcg gctgcactct ttgtgctcga aaatgtcccg
    12121 tttcctgtat ttccggaaag gtgaaacagg tgcatacaat tgatttagag aaatgtatca
    12181 aatgtggagc ttgttttgat gcctgtaaat ttgaagcggt agtaaaagaa taggagcggt
    12241 ggaaatgata gaagtaattt taaacggaaa aacagtgaaa gcggaagccg gaatctctat
    12301 tctggaactt tgccaacagc ataatattga tattcctact ttatgtaatg atgaagaact
    12361 gaatccttac ggttcctgtt gggtatgttt ggtagaagta aaaggcagaa agggatttgt
    12421 aacttcctgc ggaactacta tttcggaagg gatggagata ataaccaata gcgaagaaat
    12481 aacccgagca cgcaaaatgg ctttggaatt gctgatttcc aatcattatg cagattgcgt
    12541 tgccccctgc acaattgcct gtcccgatca agttgatatt cagacctata tttctttaat
    12601 tgccaatggt aaatatcacg aagcggtaaa agtaattaag gaaaaattgc cgatgcctct
    12661 ttccatcgga aggatttgtc ccgctttttg tgaaaaagaa tgccgcagac aaattgtgga
    12721 tgaaagtatt gctattcgtc agttaaaacg atatgctgcc gatgtggata atgaagatgt
    12781 gtggaattat gttcccgaaa agctatccac aaaaaacaaa aaaatagcca ttatcggagc
    12841 tggacccagc ggacttacct gtggctattt tctttccaat tggggctatg aagttatcat
    12901 ttatgaagca gctcctcaag ctggaggttg gcttcgttat ggaattccgg aatttcgttt
    12961 gccgaaagat attctggatg ctgaaattga attgatgtgt actaatggaa tgcagattga
    13021 atataataaa caactgggta aagatattta tttgcaaaac ttggcagata gttatgatgc
    13081 cgtatatctt gccatcggag ctcaaaaagc agttccaatg cctgtgaaag gtagcgattt
    13141 ggatggttgc tttttagggg ttgatttttt gaaggcacac gccttaggca attcacccaa
    13201 tttaggatta aaagtagcca tcgtaggagg aggaaataca gctgttgatt gtgcccgaac
    13261 cgctattaga aagggttgtg aagttgccgt catttacaga agaacgaaag ctgaaatgcc
    13321 tgctgaagca agtgaaattg ttgcctgtga aaatgaaggc gtgaaatttt tctaccttgc
    13381 caatcctatt gaatatattg gtaaaggtgg acatttaacc agcgtaaaaa tagaaaaaat
    13441 gcgtttaggc gaacctgata aaagcggaag aaaaagaccc gagcccaccg gtgaattttt
    13501 tgaactgccc ttcgatagta tcattgctgc tatttcccaa gttcctgagg tggatatctt
    13561 tacggaagag aaaaactatg ttgcggataa agtattgccc atttcgcgtt ggcaaacggc
    13621 tattgtagat gaaacaacta tgtttacagg cttggaaaat gtttttgccg gaggtgattt
    13681 tagaagaggt gccgccactg caatcgaagc aattgccgat ggaagaattg ccgctaaggc
    13741 aatagataac tatttagcaa caggagagat agttgcagag cttattcctt ttgacagtaa
    13801 aaaagctaaa agcgtgaaag atatcagtcc tgcagagtat tctatttttg aaccaaaaaa
    13861 gcggaaagaa atgccagaat tacctttggg agaagcaaaa aatagtttta aggaagtgga
    13921 acttggcttt ttggaagaag atgccatcaa tgaagcaaaa cgctgtttgg aatgtggttg
    13981 ttttgtaaat gagacctgtt ctttgcgaaa atattgtact gactatttag taaatccagc
    14041 tcactttttg gggggtatta ataagcatcc gattgattac agccatccct ttattgtgcg
    14101 cgatgccaat aagtgtataa attgtggacg ctgtattaga acctgtgccg aaattcaagg
    14161 tccagcagtt ttaggcttta tttatagagg ttttggaaca atggtagctc ccgaatttgg
    14221 tgattcttta accaaaacca gctgtctttc ctgtggaaaa tgcatagatg tttgtccggt
    14281 gggtgcttta acggaaagaa atatgcacta taaattcaat cctcatccca aagatatctc
    14341 attgcaaaat tgtgcgttat gtggagttgg ttgtgtaatt caaaccgaaa ctcaagcagg
    14401 aactattacc aaaattacta cttcgcaaaa agagcctggt tttaatggta agaatttatg
    14461 ttttaaaggt cgtttcggct ggcaaggtct ttatgacaaa gatagattga atgttcccct
    14521 gctaaagcaa aatggttatt ttaaagaaat tagctggcaa gaagcagctt ctcttatagc
    14581 tgaaaaaata gcattgtgca attcccaacg ctttgaaata agccctcaca tttctctgga
    14641 agaaatgcta atgctgcaaa gggttgccga taaatatgaa actaagcttt gtgccaatcc
    14701 tgcctttgct tattttagcg attctttcct atccttaaaa ccagaaagtg aaccctataa
    14761 aattctggaa aattatgatg aatatgttat ttatggagaa ttaaatcagg ttctatcctc
    14821 tttaatccgc ttacagcaaa ggaaaggaaa gaaattagtt ctggtaaatt atccggaaag
    14881 cccttaccgt cgttttgccg atgctaacta tgccaatttg caagaagttc aggcaaccca
    14941 gaagacactc tttgtctata atcaaaatcg gattactgaa caggaggcat ataatttatg
    15001 gtgttttgcc tctaaaatag gaactgagaa tatactggtt tctacggatt atcgtaatca
    15061 tctggggttt ttagctatga agccggattt ttctgaaaaa gttgctgatt tcgttttagg
    15121 ttggggtgta tatcctgctt tggctaatca ggcaaaattc agcattgcca taatgaattt
    15181 ccaggatgaa actgcacccg tggatttatt aattccggct cccagctttc tggaaataga
    15241 aggaacttca ctaagtgatt tagcgcagat aactaaaagt gccaatcctg ctaaatccat
    15301 cgttatcaat gaattgatgc gtttaatgta taagctgaac tggattcatc ccaatagcgc
    15361 agaaattcct ttctggaatt atgaagcgga gaagttgctg attagtttgc aaaattatgc
    15421 tcctaataag tttgaaccct cctttattaa gcctgaggat ttgaataaag ggattaaggt
    15481 tatccctgcg caggcagaaa aatatattgc tgcccttttt gaattgcgcc aaaagcccac
    15541 aagcttttaa tatatgtttt tcacgcagat tttgcagata acgcagattg gattttagta
    15601 acaaggattt taggatttta ggattaagag gatgtaaaac aagagtccga aagcactttg
    15661 ataacttgtg cctttatcta acttttaagt gcatatagtt aataatgaca ataatttatt
    15721 gtgtagccgg aggacgcctt ctcgacccct cgactcctcg acccctcgac ccctcgacct
    15781 ctcgacctct cgaccccttt ccacttttcc actttcttgc ggagcttcag cgagctccgg
    15841 ctacacaatt tctgccatca cagcatctct tttggctttg gataaagaat gtagttcata
    15901 gcccagctct ttatgttttt gctgaagagc cgttaaaatt tcggcagccg aacgattttg
    15961 cagcatttct tgccactctt ttttgtgttc tcctttattc caatacagat ttaaaagttt
    16021 ggcaaagctc tccaattgat gcatttcttc aaaagagagg gcattggaag atagacattg
    16081 gtagggtggc tgatgtagcc atttatagtt taatgctata gctatttctt gcatagatgt
    16141 atcaggcaaa atttttagga tgcccagctg aacttcatcc ggaagagtag cacagacctc
    16201 attcaaacca tttattatag agaaataatt ttctcctggc aaaccggcaa gtaagtcagt
    16261 atgaactcgt agtttagttc tttttttcag ttctcttaaa acccattttg ctttttccca
    16321 atttgatttt ctgccacttt ttgctgcaat ttcagcacta attgtttgaa tgccgatttc
    16381 caaccggatt ctattttccg gcgctttttc caagagggca atatcctctt cttcaatcaa
    16441 atcgggatag atttcaaaat ggacatcgca agggcaattt tgctcgataa aaaattgcca
    16501 gatataatgt gctaaccttt tgttaatatt aaaactgcgg tcaacgaatt tgatggttct
    16561 tggctctaaa gcaattaggg cttccagttc tttctttagg cattgcatat ctttttgatt
    16621 ttcaggatga tagcgaagtt caaagcgttt atctttagct gaaagacaat agatgcaggc
    16681 aaaaggacag ccccgaaatg tctcgtagta aagcaatttg ccttttagaa attcagcgtc
    16741 actttgtcta tagggaaaag gaatgtcttt caagggaata tgctttgcct gggttaattc
    16801 ttcttctttt aagcaaaagt tgttttctgc caggtagcga aatttacctt cgccagctcc
    16861 ttggataaag taaaatctat cccgttgcag ataagtgaga tttcccgctt ctggaccacc
    16921 tattacaatg attgcttctg gcaaaagttt taccagctca ggaattaatt cctgcagata
    16981 aagacgattc cagatatagg cagaaaaaca tattacatca gcattttgaa cataaattcc
    17041 ataaagcaca tccagcaatt gatcttttaa agtccattcc tgcataaata ccctatatgg
    17101 cagatcggca ataacttccc gtaaatagta taaagccaga ttggattgac tccagctgct
    17161 atttatggct gtcattaata ttttcttcat ttatctttac ctttttctta tcttatattc
    17221 taaatctgtt ggtagttgtc aatgaaaatg atgcaaacaa gtggtctttg ctgaagagga
    17281 ttaattttaa tagtttgcta tctggttgaa acttaaaatg atactgcaat tcccttgcta
    17341 acaacccgtc ctcctctcct gctcctctcc tcctccaccc atcgtttagg aggagagaag
    17401 atggggtgga tgacaagctg tatat
//
LOCUS       DAOSTH010000030        17330 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3414_length_17330_cov_16.301881, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000030 DAOSTH010000000
VERSION     DAOSTH010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 17330)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17330)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17330
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3414_length_17330_cov_16.301881"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..785)
                     /gene="frr"
                     /locus_tag="PLB65_05010"
     CDS             complement(231..785)
                     /gene="frr"
                     /locus_tag="PLB65_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOQ77923.1"
                     /translation="MEANKEITKEKMQKSFDSMLHQYSKIRTGRASASILDDIKINYY
                     GQPTPIKQLCNISIPEARMIVVQPWDKTTLADIEKAILAANIGITPENDGNVIRLPFQ
                     PLTEEKRRDIVKSIKKISEDTRVAIRNIRRDSNELVKKLKKDSEISEDEEKKQLKELQ
                     DITDDWIKKIDEAEKAKEKEIMEV"
     gene            complement(821..1549)
                     /gene="pyrH"
                     /locus_tag="PLB65_05015"
     CDS             complement(821..1549)
                     /gene="pyrH"
                     /locus_tag="PLB65_05015"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOQ77924.1"
                     /translation="MKVTYKSENIHRVLLKLSGEVLSGKKGIAYDEALINSLTDAIIS
                     VHNQNYELGIVLGGGNIFRGGTWKNKKLNRVVLDSIGMLATIQNSLYLAEVLGSKGIE
                     SVVLSTLAVDKVVDRYTPQLALSAMEQGKICFLSGGTGNPYFTTDTAAVLRAVELQAD
                     VVLKATNVDGLYSADPKKDKNAHFIKSASFQQCVQNRLGVMDLTAFSLAMDNEMPIKI
                     FNISNPDMLIEALTNSEVGTYIHP"
     gene            complement(1601..2200)
                     /gene="tsf"
                     /locus_tag="PLB65_05020"
     CDS             complement(1601..2200)
                     /gene="tsf"
                     /locus_tag="PLB65_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOQ77925.1"
                     /translation="MAEITAAQVKELRDRTGAGMMDCRKALIEKKGNIDEAIKYLRER
                     GISKAEGKASRATKEGVIHSYIHFNNRIGVLLELNCESDFVARTPEFKALADEIAMQI
                     AATNPLAISPEQIDPAILDREKEIAYNKAVNEGKKPEIIEKIVEGNLKKYCSEHSLME
                     QELISDSTKTVKDLLTNAIATTGENIQIARFIRYQLGGE"
     gene            complement(2391..3101)
                     /gene="rpsB"
                     /locus_tag="PLB65_05025"
                     /pseudo
     CDS             complement(2391..3101)
                     /gene="rpsB"
                     /locus_tag="PLB65_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
     gene            complement(3250..3636)
                     /gene="rpsI"
                     /locus_tag="PLB65_05030"
     CDS             complement(3250..3636)
                     /gene="rpsI"
                     /locus_tag="PLB65_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOQ77926.1"
                     /translation="MQTFDAVGRRKNAVARVRLTPGTGKRIINNIHMKKYLQRETLEM
                     IVEQPLQAVGLSDNFDVYANVCGGGLSGQAGAIRHGISRALVEYDENLRPILKPRGFL
                     TRDPRMVERKKSGRPKARKRFQFSKR"
     gene            complement(3647..4075)
                     /gene="rplM"
                     /locus_tag="PLB65_05035"
     CDS             complement(3647..4075)
                     /gene="rplM"
                     /locus_tag="PLB65_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOQ77927.1"
                     /translation="MKTLTPSPSDIHQKWYIVDAEGKPLGRLSTKVATILRGKHKPYY
                     VPNIDTGDYVIVINAAKVHVSGMKALQKVYKSYSGYPDGLKEVPYAKVLEKHPERIIE
                     HAVKGMMPKNKLGRAMFKKLKVYPGAEHPHSAQKPIELEI"
     gene            4525..5316
                     /locus_tag="PLB65_05040"
     CDS             4525..5316
                     /locus_tag="PLB65_05040"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOQ77928.1"
                     /translation="MKKYLITGLLLLVIVFAFAERKALVIANWNYPNAVLKSPKADAD
                     SIAAALEGVNFNITRLNNANLNSMKSAIDTLAAHLSSEDEVVFYFSGHGTNYKNVNYL
                     VPAGVNLASQLTIDNNCYSLSALSNKLKNAKTSIIIVEASKIWGIPDGKATNPKTFVS
                     MKSATPKQVIIFSSQPGKIVQNSNLTKSVFSMAMAKYIATSEVGLNEFFPVLSNEIMQ
                     KTGNAQKPWISGTLSNDFIFNTNLQKGMWKSPSPRPKIGGGSLSW"
     gene            5655..6338
                     /locus_tag="PLB65_05045"
     CDS             5655..6338
                     /locus_tag="PLB65_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ77929.1"
                     /translation="MDIIKTEDIVKDYILGKVKVRALNGVNLHIQKGEFVAIMGPSGS
                     GKSTLMHILGCLDKPTAGEYYLDDILVSKMPKAALAAVRNRKIGFVFQSFNLLPHLNI
                     LKNVELPLMYGGMSRHKRLAKAREVLQNVGLGNRLKHKPNELSGGQRQRVAIARAIVN
                     DPSILLADEPTGNLDSQSGGDILEIFKDLHSQGNTVIIVTHDQAIASRAERIIRIKDG
                     KIVDGNSDS"
     gene            6319..7554
                     /locus_tag="PLB65_05050"
     CDS             6319..7554
                     /locus_tag="PLB65_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ77930.1"
                     /translation="MAIPIAESLKLGIADILMRKVRSIVTVIGIILGVMCIMVVLAIV
                     NGMNKSTMSWMEERGGLSKIEVEQNWQYDFSKGGDPSFTLKEIRYLQSLLPEAEAFNP
                     SVQEWEAIITKGDIRYSTSLRGVMPDFVKVEQWDIAKGRFIKDMDIDLHSNVVVVGST
                     VAKEFFGNSEPLGQIMSIGNQQFTVVGVMSERFMQSQGGQIGMGDNALEYLNQRCFIP
                     ISTMISKVNPISKISSIDIRAKSPEEAKELRRKVENIVLNLKNGKRLFQVTSAKEQMD
                     TMQANAKIMEAIFILIAVISLLVGGIVIMNIMLASIRERTREIGVRIAVGARSRDIFL
                     QFLVQTILITSLGGVLGILFGFAILDKVGSYLQIEVLASVQMIWVALLVSIGVGLIFG
                     VGPAIRASRLDPVIALREE"
     gene            7554..8636
                     /locus_tag="PLB65_05055"
     CDS             7554..8636
                     /locus_tag="PLB65_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HOQ77931.1"
                     /translation="MSKKKQKSSFFHKLFLWLYSYINVVIVDIKKIYQALTVAQQRKL
                     IINNIWNFIKNFYQRFMGEGILKESASLTYITILGFVPFINFLVLIAPDLPFLNLRDK
                     LRELVIENFIPSSANAIISFLDKLIMPKVGFNIFNFVILIVSSYSLFNVIRSTFDRIL
                     SMQLKTSQDTITQLVKFFGTVVFGLLIIVLLFSSSSIPIISRLLKSRVLQWIMVIVPF
                     VLQFLAIMFLYMLLPSVRINRGALFRGAFWTTVIWVIAKSAFDFYIYNLTNYQALYGV
                     MAVLPIFLLWIYINWAIILGGMVLVSVIENKKTGGFLTKEPHNAVRITMELFTNHKLN
                     QRLEGLLSKDDLKKLVETLNEEEEDK"
     gene            8633..10174
                     /gene="purH"
                     /locus_tag="PLB65_05060"
     CDS             8633..10174
                     /gene="purH"
                     /locus_tag="PLB65_05060"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HOQ77932.1"
                     /translation="MKKYALISVSDKTGIENLAVELEKLGYTILSTSHTAEYLKKYCQ
                     QVVLVSDLTKFPEILEGRVKTLHPIIYAGILADRQNPSHEKTLSELNIDHIDVVAVNL
                     YPFAAVRKKENASEQEIIENIDIGGPALIRAAAKNYRNVTVLVDPMDYNHTLELLKEN
                     SILPKNYSAYLAKKAFSKTRDYDSEIAAYFMEMETVAKDAIPFQLSISASLNQNLRYG
                     ENPHQNAGYYTNTSAAWEVLHGKELSFNNIMDIDSALRAIRLFSEPTAIIVKHCNPCG
                     IGSGEKLAEAYTKAFATDTEAPFGGIVIVNRSLDLETATLINNIFTEIIIAPDYEPGV
                     LEFLKKKKNRRLIRYDLSLLAKPINPIEIKTLTFGYLAQNWDLVNESIENWKIVTEKQ
                     PTSEEFQALIYAWKAVSILKSNAIAIAKKDRTLGLGCGQTSRIDAVQLAIWKAKKFNH
                     ILEGSVCASDGFFPFRDCIDVLVKNGISAIIQPGGSKNDEESIAACNELGITMLFTGF
                     RHFRH"
     gene            10387..10477
                     /locus_tag="PLB65_05065"
     tRNA            10387..10477
                     /locus_tag="PLB65_05065"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10422..10424,aa:Ser,seq:gga)
     gene            10606..12324
                     /gene="sppA"
                     /locus_tag="PLB65_05070"
     CDS             10606..12324
                     /gene="sppA"
                     /locus_tag="PLB65_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HOQ77933.1"
                     /translation="MKSNKTFLWGCLIFLVAFIAACVLGFIFTFGSAVPKSAKIPTNA
                     WLYLNPSAMISDYNEIEDVKFFSVTSFSVQEINSKIIAAAKDKRIKGLLIEPKMIMTN
                     YPALGEMSLAIETFQKSGKPIVAFGENFSQADYLLASCADKIYMEPSASAGLALQGVS
                     ANMMFYKEMLDKLGIKMHILQSGAYKGAGEPYSQMELSKGTKDNIDAALQGIYNHLLT
                     FVAKNRKLDPAEVKDIFENRDDFFLTANKAKDLKLIDYAMGRDEMLSNLGLSEDNFIK
                     IGSYSPSSPKSKDSKIAVVYLNGNITPSTNYEFSSQNYISSAKVKKICKQIRDDKDVK
                     AVVLRINSPGGSALESELIYQQLIKLKKDYPLVISMGGTAASGGYYISCAGDYIIADP
                     GTLTGSIGVIGLIPETVGLGKKIGVRSQTIKYGKFAGAFNLFESYDSALIESLKRNST
                     GTYNEFQQRVMSCRNITPEQIESVAEGRIFNAEDALENNLIDEIGTLDKAINKAASLA
                     KVTTYSSTNYPKKASLMEVLKESNLLNVKEQIQISNTPELQMEKYLKQISPTGEWLYL
                     MPLKLD"
     gene            12326..13843
                     /locus_tag="PLB65_05075"
     CDS             12326..13843
                     /locus_tag="PLB65_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOQ77934.1"
                     /translation="MKKDSYNTEIIVNVHPLEKRVAVLEDNRLVELFVERREQQNIVG
                     NIYKGIVKDVLPGMGAAFIEIGLERTAFLHYSDIVMDFLDIYESDQPRTKVNPEDSSQ
                     IDKLLKPGQEIVVQIHKGPIGSKGARLTGQISIPGKYLVLFPNKDKIAISRKVYSQNE
                     RNRIRNLLSEIKDPGYGLIVRTEAEGCEEDDFVTEYKALLKTWRLIEKQLKYAKAPVC
                     VFEENALENYLIRDLFGENVDRLVIDDKNFARRIISQLEDISPELISNIEIYREDSPI
                     FDAWGIEKKIETGLYSRIYLPSGGNIKIEQTEALVAIDVNTGSFTGKTHYNETIRKTN
                     LEAAAEIARQIRLRDLSGVIVVDFIDMVEEKHKTEVLDMLKKGLRRDRAKNKVYPFTE
                     LGLVEITRKRMRNTLISNFSDPCPFCNGSGRILSKNAVIMRIYRWLNRGDYFVKNKNL
                     RIMVHPDLLTHISQHSEDFVAYENQIEFAAEPSIRIDQFKVYTLPDLEEITSKFS"
     gene            complement(14303..14593)
                     /locus_tag="PLB65_05080"
     CDS             complement(14303..14593)
                     /locus_tag="PLB65_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOQ77935.1"
                     /translation="MRFGQNRLRFRHWGKYKAFTCEPNQCLALDQIPEGYNATVIHNN
                     NLKTIERGLYLGAPITVFRNNIDEPNIIVAVGDARYVLDRRIARTIKARISQ"
     gene            complement(14574..16754)
                     /gene="feoB"
                     /locus_tag="PLB65_05085"
     CDS             complement(14574..16754)
                     /gene="feoB"
                     /locus_tag="PLB65_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HOQ77936.1"
                     /translation="MRNRPIIALAGNPNVGKTTLFNALTGSRQTVGNWPGVTVEHKSG
                     ICSFNNKVYEVIDLPGIYSLAGESPDELVARNYILSEKPDIIINIVDASNLERNLYLT
                     VQLMELGAPLIMCLSMVDIAEHNGIVIDTQHLSSHLGFAVIPLVLNKRIDVSRILEKV
                     EEYVLQPPVPDTIHYDEVVEKHLENIWQIVLKAGLEDPEKSVSGRFKKDAALNAYAIL
                     IKSRWQALKLIEGDAEINSHLTEEERSLVEKERMAIAKHRGQEPAVVIADDRYAYIRG
                     LIKDVVKRKRKSRWTFSDMVDKVVLNSYIGLPIFFIVMYLVFLVAVKLSQPCIEIINV
                     YLGWLFVEQFGKLLTLMSVPQWLIYFFSEILGGGLVTIGSFIPPIFFIYISLSILEDS
                     GYMARAAFIADKFMRKIGLPGKAFIPLIVGFGCTVPAIMATRTLEKPRDRVFASILTP
                     FISCGAKLPVYTYLVMLFFPQRADIVIFALYFLGIIMGILTGLLLKATIFKSAPGDFV
                     MELPAYHIPTINGIFMHTWHRLKSFLLRAGKTILLVIVIINILQVIQIPVGNKNEKTP
                     LLELGGKLITPVLQPMGIKNDNWPASVALISGLFAKEAIVGTLQGLYQNSEDEGAIKH
                     LTDNIKKGFGSNASVFAYLVFILLYSPCAAALTMLFKEHGFKWMLFDFGYLTVLAWMV
                     ATLVYQILAFNALSIFWFAVIGVIFIIIYINLRSIGSRYAIRAK"
     gene            complement(16744..17202)
                     /locus_tag="PLB65_05090"
     CDS             complement(16744..17202)
                     /locus_tag="PLB65_05090"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HOQ77937.1"
                     /translation="MEEYADIFRKYLDKQGLKFTQPRRYILDEVFQMHTHFNAEELYD
                     RIRSKTNEISLATVYRTIPLLLEAGLVQRADRSEGRERYEHIFGHPKHIHWVCRNCGA
                     LLETDLNTLYPALAKQAKDLKFKPEDIELSVKGLCWKCNPNENENQSDEK"
BASE COUNT         5357 a         3243 c         3117 g         5613 t
ORIGIN      
        1 ttttttcgcc gggttaaaac ccgtcgttag tatgtatcgt tcctaacgga actaatctct
       61 ttatgtaaat ttgtttcgag ggcttatgct ctatcgctat gattgtgtcg gcctgagggg
      121 cttttattgg ctatataatt caatcctgta aatccctttc atcttgttca tcccatacct
      181 attctttttt taatctgcgt aatctgcgta atctgcgtga gataaaccct ctacacttcc
      241 attatctctt tttcttttgc tttttctgct tcgtctattt ttttaatcca gtcgtcggtt
      301 atatcttgga gttcttttag ttgttttttt tcttcatctt cgctgatttc gctatccttt
      361 ttcagttttt taaccagctc attagaatcg cggcgaatat ttcgaatagc aactctggta
      421 tcttcggata tttttttgat gcttttcaca atatctctgc gtttttcttc ggtcaggggt
      481 tgaaaaggta ggcgaataac atttccatca ttttctggag taatcccaat attggcagct
      541 aagatagctt tttcaatatc tgccagagtg gttttatccc aaggttgaac aacaatcatt
      601 ctcgcttctg gaatggaaat attgcatagt tgttttatgg gtgtaggttg gccgtaatag
      661 ttaattttaa tatcatccag aatggaagca ctggcgcgtc cggttctgat tttggaatat
      721 tgatgcagca tagaatcaaa gcttttctgc attttttctt tagtaatttc tttattagct
      781 tccataataa cctctttaat attagcttag tttatctgtt ttaggggtga atataggttc
      841 ccacttcgga attggtgagg gcttcgatta acatatccgg attactgata ttgaaaattt
      901 taataggcat ttcattatcc atagccagag aaaaagcagt taaatccata actcccaaac
      961 gattctgaac acattgttga aagctggcac ttttaataaa atgggcattt ttatcttttt
     1021 taggatctgc actatatagt ccatctacat tagttgcttt taaaacaaca tcggcttgaa
     1081 gctcaacagc tcttaaaact gctgctgtat cagtagtaaa ataaggattt cctgttccac
     1141 cacttaaaaa acagatcttt ccttgttcca tagcgcttaa agccaattgt ggagtgtaac
     1201 gatcaacaac tttatccaca gccagagtgg aaagaacaac actctcaatt cctttagaac
     1261 caagaacttc tgccaagtaa agggaattct ggatagttgc cagcatacct atactatcca
     1321 gaactaccct gtttaatttt ttatttttcc aggttccgcc cctgaaaata tttccgccac
     1381 ccaaaactat acccaattca taattctgat tatgaactga aattatagca tcagtaaggg
     1441 aattaattaa tgcctcatca taggcaatac cttttttccc ggaaagaact tctccggaga
     1501 gttttagcag caccctatgg atgttttcgc ttttgtaagt aactttcatt aactaactcc
     1561 ccactgtatt tgtatgagtt gcaataatta tctgtctatt ttattcgcca cctaattgat
     1621 agcggataaa tctggcaatc tgaatgttct cacctgttgt agcaatagcg ttagttagca
     1681 agtccttaac tgttttagta ctatcactga tcaattcttg ttccattaag gaatgctcac
     1741 tacagtactt tttcaggttt ccttccacaa ttttttctat tatttcaggt ttcttacctt
     1801 cgttaactgc tttattataa gcgatttctt tttccctatc caaaatagca ggatcaattt
     1861 gttcaggact gatagctaaa ggattagtag cagcaatctg catagcaatt tcgtctgcca
     1921 aagctttaaa ttcgggagtc ctggctacaa aatctgattc acaattcagt tccagaagca
     1981 ctccaatccg gttgttgaaa tgtatgtaag aatgaataac accttcttta gtagcacggc
     2041 ttgcttttcc ttctgcttta ctgattccgc gttcccgcag gtattttatt gcctcatcaa
     2101 tgtttccttt tttttcaatt aaagctttgc gacaatccat cattcctgcg ccggttctat
     2161 ctcttaattc ttttacttgt gcggcagtaa tctcagccat ttttttctcc tttttaaata
     2221 ggggaataaa ttcactttac cttgtagcaa agctatttct tcccccgatt atttgtgctt
     2281 tatagttctg tttccgttaa ttcgggaact tcaaattctt ctttttctcc tgatactgcc
     2341 aattgagctt caaaatctcc tggttcttct tctgtaagtg cttcttcatt ttctgcttca
     2401 atgttttcac cttctgaggc aagaccacgt ccctcaataa ctgcattagc cataatatca
     2461 gaaattagat taatggcacg agtagcatca tcgttactag gtataacata atcaataata
     2521 tccggatcgc aatttgtatc taccatagcc acaatcggaa tattcaaaat gcgtgcttcg
     2581 tgaacagcta ttttttcata ttcagtatcc acaataaata tacatcctgg aagggcatcc
     2641 atctcgcgaa ttccacctaa ggaaaattct attttatcgt gcattctttt cattttttgc
     2701 tgttccagtt tggtataact gtttatagtt ccatcagcaa cgatttcttc ataatatttc
     2761 attttatcta tgctttgacg aatagttgcc atattggtta gcattcctcc ataccaacgt
     2821 tggttaacat aaaaaactcc agctttttca gcagcttctt taattgctgc ttgtgcttgt
     2881 tttttggtcc caacaaaaag aatatactct cctttggagg caacttcttt aataaactga
     2941 tatgcttcat taatagcatc aacggtctgc ttaagatcaa tgatgtgaat cccattgcgt
     3001 ttaatgaaga tatacttttt cattttggga ttccatttaa aggtctgatg cccaaaatgg
     3061 acacctgcct cgagtaattg tttcatagta actacggaca ttttttctcc tgatagacgg
     3121 ttattaagtt tcttccggac ggtgtaagca attcagagaa aatttttcct caccgccttg
     3181 caagcgttcc ggaagtttat ttttggttaa tagtaattgt ttataactca taaagaataa
     3241 actgactaat caacgcttgg agaactggaa tctctttctg gctttgggac ggcctgattt
     3301 cttgcgttcc accattcggg gatcgcgagt taaaaagcca cgaggcttaa gtatcggacg
     3361 taagttttca tcatattcaa ctaaggcacg agaaatgcca tgacgaatag ctcctgcctg
     3421 accacttaat ccgccgccac acacattagc ataaacatcg aagttatcgg ataaaccaac
     3481 agcttgtaag ggctgttcaa caatcatttc cagggtttct ctctggaggt attttttcat
     3541 atggatattg ttgataatgc gttttccggt tcccggcgtg agccgaactc tggctacggc
     3601 attttttctt cttccaactg catcaaaagt ctgcatattt tttctcctaa atctcaagtt
     3661 ctatcggttt ctgagctgag tgtggatgtt cagctcccgg ataaactttc agctttttaa
     3721 acatagcgcg tcccagctta tttttgggca tcataccttt tacggcatgc tcaataatcc
     3781 gctcgggatg cttttccaaa actttagcat aaggaacttc cttcagacca tccggataac
     3841 cactatagct tttataaacc ttttgcaatg ccttcattcc gctaacatga actttagctg
     3901 cattgataac gattacataa tcgccagtat caatattggg cacataatac ggtttatgtt
     3961 ttccacgtaa aattgtggct acttttgtgg ataagcggcc taagggtttt ccttctgcgt
     4021 caacgatata ccatttctgg tgaatatcgg aagggctagg ggtaagggtc ttcatcgctt
     4081 ctcctttata tatttttcta ttcagatagc caaaattttg gaatatagga tagtgtcaag
     4141 aactgttttt tcaaattttg gaaaagtaga gagaataaag aacttaaatg aagacagaag
     4201 aattacgata gataggcgag attaccaagc cttagaaaat agctattgca tagtactatt
     4261 ggtaataaaa taaacatttt gcttaccaaa aaactatctt atcctcagag ctgatgaata
     4321 taaaagttat tttacaggaa cctctaatag gtcggtttta tcatttattg ttggtttgca
     4381 tatataatat atcctaaaag ctcgttacca aaattagtat ctgcctaatt tatgatgaat
     4441 ctaccccatt ttgttcataa attgattgac ataataatgc ttccctttct atagtgaaat
     4501 ttgatgaaaa atatggagga taaaatgaag aagtatctga taacaggtct tttattgctg
     4561 gtgattgttt ttgcttttgc tgaacgcaaa gccttggtaa ttgccaattg gaattatcct
     4621 aatgcagtgt taaaaagtcc aaaagccgat gctgattcaa ttgctgctgc tctggaagga
     4681 gtgaatttta atattacccg acttaataat gctaatttga attcaatgaa atctgctatc
     4741 gatactcttg cagctcacct aagcagtgaa gatgaagtag ttttttattt cagcgggcac
     4801 ggaacaaact ataagaatgt caattactta gttcctgcag gagtgaattt agcatctcag
     4861 ctaactattg ataataactg ttacagttta tctgccttaa gcaacaaatt aaaaaatgcc
     4921 aaaacctcaa tcataattgt ggaagcatcc aaaatttggg gtattccaga tggtaaagca
     4981 actaatccca aaaccttcgt aagtatgaaa tctgctacgc ccaaacaggt aattatattt
     5041 agtagccagc caggaaaaat agtacagaat tctaatttaa ccaaaagtgt tttctctatg
     5101 gctatggcta agtacattgc tacctcggaa gttgggttaa atgaattttt cccagtattg
     5161 agcaatgaaa ttatgcaaaa aacgggaaat gctcaaaagc catggatttc aggcacatta
     5221 agtaatgatt tcatctttaa tactaatctc cagaaaggaa tgtggaaatc tccctcccct
     5281 cgtcctaaaa tcggaggtgg ctcactttcc tggtaacctt attgaaactc agttaactgg
     5341 aaaaaatgaa tttcttaatt taggagattc gtttaacact tatttcacag atatattata
     5401 gctaggaaca taacagctac ttccgttgta attttattgt aagcaatgaa gttatcaaag
     5461 cgtaaatcat ttctggtttg tatgtaagtt gtgattcttc atttccgcag tattttagcc
     5521 attctataaa ctcttgggac agtaaggact catatcgatg gctgattata tctcactatt
     5581 tcatcatcac attttttcat tgacacaaag tttgattcta agtagtttga ctatacaagg
     5641 aagaatagat taatatggat attattaaaa cagaagacat cgtgaaggat tacatcttag
     5701 gcaaagtaaa ggtccgcgct ttaaatggag taaatcttca tattcagaaa ggtgaatttg
     5761 ttgccattat gggtccctct ggttcaggta aaagcacttt aatgcacata cttggttgtt
     5821 tggataagcc tacagctgga gaatattatt tggatgatat tctggtgagc aaaatgccta
     5881 aagcagcttt agctgcagta agaaatagga agattggctt tgtttttcaa tccttcaatc
     5941 ttctcccgca tttaaatatc ttaaaaaatg tagaattgcc cttgatgtat ggagggatga
     6001 gtcgtcataa acgtcttgcc aaagccagag aggttttaca aaatgttgga ttgggaaatc
     6061 gtttgaaaca taagcccaat gaactttctg gcggtcaaag acaacgagtt gccatagcca
     6121 gagcaattgt taatgaccca tcaattttac ttgccgacga gccgacgggt aatcttgatt
     6181 ctcaatctgg gggagatatc ttagaaattt tcaaagattt acatagtcag ggtaatactg
     6241 ttatcatagt tacccacgac caagcaattg cttccagagc agaaagaatt attagaatta
     6301 aagatggaaa aatagtagat ggcaattccg atagctgaat ccctaaaact tggtatagca
     6361 gatatattga tgcgtaaagt ccgcagcatc gtaaccgtaa ttggtataat tttaggtgtg
     6421 atgtgtataa tggttgttct ggccattgtt aacggaatga ataaaagcac tatgagctgg
     6481 atggaagaac gcggaggctt aagtaaaatt gaagtagaac aaaactggca gtatgatttt
     6541 tccaagggag gcgatccgag ctttacttta aaggaaattc gttatttaca atctcttctt
     6601 ccagaagcag aagccttcaa tccatccgtc caggaatggg aagcaataat tactaagggt
     6661 gatattcgtt acagcacttc tttaagaggt gtaatgcctg attttgtaaa agtagaacag
     6721 tgggatatag ctaaaggcag attcataaag gatatggata tagatctaca cagcaatgta
     6781 gttgtagttg gctcaactgt agctaaggaa ttttttggta acagcgaacc cttaggtcaa
     6841 attatgtcta ttggtaatca gcaatttaca gtcgtaggcg taatgtcaga aaggtttatg
     6901 caatctcagg gtggacaaat tggaatggga gataatgctt tagaatattt aaatcagcgc
     6961 tgttttattc cgatctccac tatgattagt aaagtaaatc ctatcagcaa aataagctct
     7021 attgatatca gggcaaaaag tccggaagaa gcaaaagagc taagacgcaa agtagaaaac
     7081 atcgttctga acctgaaaaa cggaaaacgt ttgtttcagg ttacctctgc taaagagcaa
     7141 atggatacta tgcaggcaaa tgctaaaatt atggaagcta tatttatttt aatagctgtt
     7201 atttctttac tggtaggagg aatcgtaatt atgaacatta tgcttgcttc tattcgagaa
     7261 cgaactcgcg aaattggagt tcgcattgca gtaggagcac gcagtagaga tatttttctg
     7321 cagttcttgg tgcaaacgat tcttattacc tctttgggtg gagttttagg aattctcttt
     7381 ggatttgcta ttttggataa agtgggttcc tatttacaga tagaagtgct tgcctcagtc
     7441 caaatgatct gggttgcgct tttggtatct attggtgtgg gacttatttt tggtgtagga
     7501 ccagcgatca gggcttcgcg tcttgatcct gtaattgctt taagagaaga ataatgagta
     7561 aaaaaaagca gaaaagcagc ttttttcata aactctttct ctggctgtat tcttatatca
     7621 atgttgtgat tgtagacata aaaaaaatct atcaggcatt aactgtagcc caacagcgga
     7681 aacttattat aaacaatatt tggaatttca tcaaaaactt ttaccagcgt tttatgggcg
     7741 agggtatttt gaaggaatca gctagtttaa cctatatcac tatcctaggt tttgttccct
     7801 ttataaattt tctggtatta atagctcccg atttaccatt tctaaatctg cgagataaac
     7861 taagggaact ggtaatcgaa aacttcattc cgagctcagc aaatgctata ataagctttt
     7921 tggataagct gattatgccc aaagttggct ttaatatctt caatttcgtt attttgattg
     7981 taagctcata ttcactgttc aatgtaatca gaagtacttt cgaccgtatt cttagtatgc
     8041 agttaaaaac ttcacaagat actataacgc aattagtaaa attcttcggc acagttgttt
     8101 tcgggttgtt aattatcgtg ttactctttt ccagttcatc cataccgatt atttcccgtt
     8161 tattaaaatc ccgcgtttta cagtggataa tggtaattgt tccttttgtt ttacaatttt
     8221 tagctataat gtttttgtat atgctactgc cttcagttag aattaatcgt ggtgcactat
     8281 ttagaggtgc tttttggact actgttatct gggtgattgc taaatctgca tttgattttt
     8341 atatatataa cttaactaat tatcaggcac tttatggagt tatggcagtg ctgccaattt
     8401 ttctgctatg gatttatatt aattgggcta ttatcttggg aggaatggtt ttggtaagtg
     8461 taattgagaa taagaaaact ggtggtttct taactaaaga acctcataat gcagttcgca
     8521 taacaatgga actatttacg aatcataaac ttaaccagcg gttggaaggt ctactaagta
     8581 aagatgatct aaaaaaactg gttgaaaccc tgaatgagga agaagaagat aaatgaaaaa
     8641 atatgcatta attagtgttt cagataaaac tggcatcgaa aatttggctg tggaactgga
     8701 aaaacttggc tacactattc tttccacttc ccatacggct gagtacttaa aaaaatactg
     8761 tcagcaagta gtgttggttt ctgatttaac caaatttccg gaaattctgg aaggaagagt
     8821 taagacccta cacccaatta tttatgccgg aattttggca gaccggcaaa atccttctca
     8881 cgaaaaaacg ctttctgagc tgaatattga tcacatagat gttgtagccg taaatttgta
     8941 tccttttgcc gccgttcgta aaaaagaaaa tgccagtgaa caagagataa ttgaaaatat
     9001 agatattggc ggtccagctt taattagagc cgcagccaaa aattatagaa atgttaccgt
     9061 tttggttgat cctatggact ataatcatac cttggaactg ctaaaagaga atagtatttt
     9121 gccaaagaac tatagtgctt atctggcaaa aaaggcattt agcaaaacac gggattacga
     9181 ttctgaaatt gccgcttatt ttatggaaat ggaaactgta gctaaagacg ctatcccttt
     9241 tcagttaagc atttctgcct cgttaaacca aaatttgcgt tatggtgaaa accctcatca
     9301 aaatgccggt tattatacaa atactagtgc agcttgggaa gttttgcatg gcaaggaatt
     9361 atctttcaat aatataatgg atattgattc tgctttaaga gccattcgtc tttttagtga
     9421 accaactgcc attattgtaa aacactgtaa tccttgcggc attggcagtg gagagaaatt
     9481 ggcagaggca tatacaaaag cttttgcaac agatacggaa gcaccttttg ggggaattgt
     9541 aatcgtgaat cgttcgctgg atctggagac ggcaacccta atcaataaca tttttaccga
     9601 aatcattatt gctcctgatt atgaaccggg tgtgctggaa ttcttgaaga aaaagaaaaa
     9661 ccgacgccta attcgctacg acctttctct attggcaaaa cctattaacc ctatagaaat
     9721 taaaacatta actttcggat atttagctca gaactgggat ctggtaaacg aatccataga
     9781 aaactggaaa atagttaccg aaaaacaacc tacttcggaa gaattccaag cccttatcta
     9841 tgcctggaaa gctgtgtcca tcctaaaatc caacgccatt gccatcgcta aaaaagacag
     9901 aacattaggg cttggttgtg gacaaacatc tcgcattgat gcagttcaat tagctatctg
     9961 gaaagctaaa aaatttaacc atattttaga gggctccgtt tgtgcctccg atggcttctt
    10021 cccttttcgg gattgtatag atgttcttgt taagaacgga atttctgcta ttattcaacc
    10081 aggtggttcc aaaaacgatg aggaaagtat tgctgcctgt aatgaattgg gcatcactat
    10141 gctctttacc ggtttccgtc actttaggca ttaatcgtga cagagaaagg aaaaagttga
    10201 tttctcgttc tacttgaact ggaaaccact tctttattct aaagcaacca agttcattcc
    10261 acaagtatat acttctatct ttgttcgcat ctttgatagt tatattttag ttgggactga
    10321 aatgaaacat atataattct gtttgacaga aagaggcagg gaaaaaaggt tgttaaaaat
    10381 acggaaggag atgtgtccga gttggctgaa ggagcacgat tggaaatcgt gtaaacgtgt
    10441 gagcgtttct agggttcgaa tccctacatc tccgccatta tttttctctg tagattaagc
    10501 ttaataaagg gacttatata gctattgagt ttatttaata gctgctaatt taatttgctg
    10561 tcatttcaaa tttgtttata ttatgaggta aggagtttat aaattatgaa atcaaacaaa
    10621 acctttctgt ggggctgttt aatctttctg gttgctttta tagctgcttg tgttcttggc
    10681 tttatattta cctttggttc tgcagtaccc aaatctgcaa aaattcctac caatgcttgg
    10741 ttatatttaa atccgagtgc tatgatttcg gactataatg aaattgaaga tgtaaaattt
    10801 tttagtgtaa cctcatttag tgttcaggaa attaactcca aaataatagc tgcagccaag
    10861 gataagcgca ttaagggtct gttgattgaa cccaagatga ttatgaccaa ttatcctgct
    10921 ttaggtgaaa tgtctttagc catagaaact ttccaaaaaa gtggtaaacc gattgttgct
    10981 tttggggaga atttttccca ggcagattat ttactcgcat cctgcgcaga taaaatttat
    11041 atggaacctt cagcatctgc aggtttggct ttacagggcg tttcagctaa tatgatgttt
    11101 tacaaagaga tgctggataa attgggcatc aaaatgcata ttttacaatc cggagcttat
    11161 aaaggagctg gtgaaccata ttctcaaatg gaattaagta aaggcaccaa agataatatt
    11221 gatgctgctt tacaagggat ttataatcat ttacttactt ttgtggctaa aaacagaaaa
    11281 ttggatcctg ctgaagtaaa agatattttt gaaaataggg acgatttttt tcttactgca
    11341 aataaagcaa aggatttaaa gctaatagat tacgctatgg ggcgtgatga aatgctgagc
    11401 aatttagggc ttagtgagga taattttatc aagattggct cctattctcc atcttctcca
    11461 aagagtaaag atagcaaaat agcagtagtc tatcttaatg gaaatatcac gccttcaaca
    11521 aattatgaat tcagcagcca gaattatatc agctctgcca aagttaaaaa aatatgtaaa
    11581 caaatccgtg acgacaaaga tgttaaggca gttgttttaa gaataaacag tcccggaggt
    11641 tctgctttgg aatccgaact tatctatcag cagttaatta agctgaaaaa ggactatcct
    11701 cttgttatct caatgggagg aactgctgca tctggaggat attatatttc ttgtgcagga
    11761 gactatataa tagccgatcc aggaactttg actggttcaa ttggagtaat aggtttaatt
    11821 ccagaaacag tggggctggg gaaaaaaatc ggagtgcgct cacaaactat aaaatatggt
    11881 aaatttgctg gtgccttcaa tctttttgaa agctatgatt ctgccttaat tgaatcctta
    11941 aaacgcaatt ccacaggaac ttataatgag tttcaacaaa gagtgatgag ctgtagaaat
    12001 ataactcctg aacaaattga atctgtagct gaaggacgca tatttaacgc tgaagatgct
    12061 ttagagaata acttgattga tgagatcgga actctggata aggcaattaa taaagctgct
    12121 tccttagcta aggtaacaac atacagttct acaaattatc ccaaaaaagc aagcttgatg
    12181 gaagtactca aagaatctaa cttgttgaat gtgaaagaac agatacaaat atccaacact
    12241 cccgaattgc aaatggaaaa atatctgaaa caaatttccc caaccggcga atggctatat
    12301 cttatgcccc taaaattgga ttaatatgaa gaaagattct tataatacag aaataatcgt
    12361 taatgtgcat cccctcgaaa agcgggttgc tgttttagaa gataatcgct tggttgaact
    12421 ttttgtggag cgtagagagc agcaaaatat cgttggcaac atctataaag gtatagtaaa
    12481 agatgttttg cctggaatgg gtgcagcttt tattgaaatc ggattagagc gaactgcttt
    12541 tttacattac agtgatattg tgatggattt tctcgatatt tatgaaagtg atcaaccccg
    12601 aacaaaagtt aatcctgaag attcctcaca aattgataaa ctcttgaaac cgggccaaga
    12661 aatcgttgtt caaatacaca aagggcctat cggctcaaaa ggagctcggt taacaggtca
    12721 aatatccatt ccgggtaaat acctcgtgct ttttcctaat aaagataaaa ttgctatttc
    12781 ccgtaaagta tattctcaaa atgaacgcaa tcgtatccgc aaccttctct cggagataaa
    12841 agaccctggt tatggtttaa ttgtacggac agaggcagaa ggttgtgaag aagatgattt
    12901 tgtgacagaa tacaaagccc tgcttaaaac ctggcgctta atagaaaaac aactgaaata
    12961 tgccaaagct ccagtttgcg tgttcgaaga aaatgctttg gaaaactatc tgatccgtga
    13021 tctttttggc gaaaatgtag atcgcttagt aattgacgat aagaactttg cccgtagaat
    13081 catctctcaa ttggaagata tttctcctga attgattagc aatatagaga tctacagaga
    13141 ggactcccct atttttgatg cctggggaat agaaaaaaaa atagaaaccg gactatatag
    13201 cagaatttac cttcccagcg gcgggaatat taaaattgaa caaacagaag cattggtagc
    13261 tatagatgtt aatacaggta gttttacagg aaaaactcat tacaacgaga caatccgtaa
    13321 aactaatctg gaagccgctg ctgaaattgc tcgtcaaatc cgcctgcgtg acctttccgg
    13381 agtgattgtt gtcgatttta tcgatatggt tgaagaaaaa cataaaaccg aagttctgga
    13441 tatgctgaaa aaaggactgc gtcgtgaccg tgccaaaaat aaagtttatc cttttacaga
    13501 acttggcctt gtggaaatta cccgtaaacg tatgcgcaac actctgatct ccaatttctc
    13561 cgatccttgc ccattttgta acggtagcgg aaggatctta agtaagaatg cagtaatcat
    13621 gcgtatttat cgttggctga atcgcggcga ttattttgtt aaaaataaaa atttgcgaat
    13681 aatggttcat cccgatttgt taactcacat tagccaacat agcgaagatt ttgtggctta
    13741 tgaaaaccaa atcgaatttg ctgcagaacc ttctattagg attgaccaat ttaaggttta
    13801 tactcttcca gacctggaag aaattacttc taagttttca taatacaatt ttgttacggc
    13861 atcgtggagc cttgttcacg atgctttttt ttgttttata acatttacag taagtgctta
    13921 tctttcattt taattgccta cagcagttta gttcattaga gtcctcttct tttacacaac
    13981 aatgatattc gcctgtcaac tttttaggac gcctcatttc catttaggcg atcctctaat
    14041 aatccgctaa cccttccgta acgaaactaa ggaatcgtta aaaaaaatgt tagagtgtga
    14101 taaaaggata ttatttgctg ataggataag tatgcttctt tcgcctcgtt atgtttagat
    14161 attctgttct aattcaaaat acacttcagc tattaaagaa gccgcataaa gttataatta
    14221 ttatcattct ttataaagct cttagctata gcggatagaa tctgtttaga atcagaacta
    14281 ctttgctaaa gtagcataaa ctctattgac ttattcttgc tttaatagta cgggcaattc
    14341 ttctatccaa aacatatctg gcatctccaa cagctacaat tatattcggt tcatcgatat
    14401 tatttctaaa tactgttatt ggtgctccca aatacagacc ccgttcaatt gtttttaaat
    14461 tgttattatg aataactgtg gcattatatc cttctggtat ttgatcaaga gcaagacatt
    14521 gattaggttc acaggtaaac gctttatatt ttccccaatg gcgaaatctt aatctatttt
    14581 gcccgaatcg catatctact ccctatactg cgtaaattga tgtaaatgat aataaaaatt
    14641 actccgataa ctgcaaacca aaaaatggat agagcattaa aagccaaaat ttgataaact
    14701 aaagtagcca ccatccaagc cagtacagtt aaatatccga aatcaaaaag catccattta
    14761 aaaccatgct ccttaaacag cattgttaaa gctgctgcac aaggtgaata tagcaaaata
    14821 aaaaccagat aagcaaacac agaagcatta ctgccaaaac cctttttgat attatccgtc
    14881 aagtgcttga ttgctccttc gtcttccgaa ttttggtaga gaccttgtaa ggttcccaca
    14941 attgcctctt tggcaaaaag cccactaatt aaagctaccg aagcaggcca gttatcattt
    15001 ttaataccca ttggctgtaa aaccggagta atcagcttac caccaagttc taaaagcgga
    15061 gttttttcgt ttttgtttcc caccgggatt tgaataactt gcaaaatatt aattattaca
    15121 atcaccagca aaatagtctt ccctgctctc aacaaaaagc ttttcagccg atgccaggta
    15181 tgcataaaaa tcccattaat agtaggaata tgataggcag gcaattccat cacgaaatct
    15241 ccaggcgcac tcttaaaaat agtagccttt aataataaac cggttaaaat ccccattatt
    15301 atacccaaaa aatagagagc aaaaataact atatccgctc gctgcggaaa aaataacatt
    15361 accagatatg tataaacagg tagctttgcc ccacaggaaa taaatggagt taaaattgat
    15421 gcaaaaacac ggtctcttgg tttttccaaa gtcctagttg ccataattgc cggaactgtg
    15481 caaccaaaac ctacaatcaa aggaataaat gccttacctg gaagcccgat tttacgcata
    15541 aatttatcgg caataaaagc tgcccttgcc atataaccag aatcttccag aatagataaa
    15601 cttatgtata taaagaaaat aggcggaata aaactgccta tggtgaccaa tccacctccc
    15661 aaaatttcac tgaagaaata gattagccat tgcggaactg acatcaaagt taataacttg
    15721 ccaaattgct ctacaaacaa ccaacccaaa taaacattaa taatctctat gcagggctgg
    15781 ctaagcttta cagcaaccaa aaaaaccaga tacatcacaa taaagaatat tggtaaacca
    15841 atataactat tcagtacaac tttatctacc atatcggaaa aagtccaccg gctttttctt
    15901 ttccgcttaa ctacatcttt tattaaccct ctaatataag cataacgatc atcagcaatt
    15961 acaactgccg gttcctggcc tctgtgctta gcaatagcca ttctctcttt ttctacaagc
    16021 gatctttctt cctcagttaa atgagaattt atttccgcat caccttcaat taattttagc
    16081 gcttgccacc tacttttaat taaaatcgca taagcattta gtgctgcatc tttcttaaat
    16141 ctgcctgaaa ctgatttttc cggatcttcc aaacctgcct taagcactat ctgccatata
    16201 ttctccaaat gtttttccac tacttcatcg tagtgaatcg tatccggaac tggtggctgc
    16261 aaaacatatt cctctacttt ttccaatatt cgggaaacat caatacgctt attcaaaacc
    16321 aaaggaataa ctgcaaaacc caaatgcgaa cttaaatgct gtgtatctat cacaattcca
    16381 ttatgctctg caatatctac catagataaa cacataatga gcggtgcccc taactccatc
    16441 aattgaactg ttaaatataa attccgttca agattggaag catctacaat gttaataatt
    16501 atatccggtt tttcagacaa aatgtaatta cgagctacaa gttcatccgg actttcccct
    16561 gccagagaat agatcccggg aagatctata acttcataaa ccttattatt aaagctgcat
    16621 atcccagatt tatgctctac tgttactccc ggccaattgc caaccgtttg tctggaacca
    16681 gttaaggcat tgaaaagcgt ggttttgcca acatttggat ttccagcaag tgcgattata
    16741 ggtctatttc tcatctgatt gattctcatt ctcatttgga ttacacttcc aacataatcc
    16801 ctttacgctc aattctatat cttcgggctt aaactttaaa tcttttgcct gtttagctag
    16861 cgcaggataa agcgtgttca aatccgtctc taacaaagca ccacaatttc tgcagaccca
    16921 gtgaatatgt tttgggtgac caaaaatatg ctcataacgc tctctgcctt cactacgatc
    16981 cgctctctgt actaaccccg cttccagcaa taaaggaatt gttctgtaaa ctgtcgccaa
    17041 agatatttcg ttggtcttgc tcctaattct gtcataaagt tcttcagcat taaaatgagt
    17101 atgcatttga aagacctcat ccagaatata acgacgcggc tgagtgaact taagtccttg
    17161 cttatcaagg tattttctaa atatatcagc atattcttcc attattttgt ctcctactat
    17221 attgagaatc atttacaata tgattcagat gcccttattt gtcaagtgcg attttttccc
    17281 ctccgctaag ttgtttattt tatatctgtt ctctttatac atcgagagct
//
LOCUS       DAOSTH010000031        17325 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3416_length_17325_cov_12.526925, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000031 DAOSTH010000000
VERSION     DAOSTH010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 17325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17325
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3416_length_17325_cov_12.526925"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..1042)
                     /locus_tag="PLB65_05095"
     CDS             complement(311..1042)
                     /locus_tag="PLB65_05095"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ77938.1"
                     /translation="MKKLSLLVLSLSLLLGSCNFFTPTERNTPIICLTFDDQRIGVYT
                     YALPEMNIYGYRGTCYVNSFYMSTRYSMNLAQVQSLHNDYNWEIGGHSLKHEDLAELT
                     YDEVEYSINQDYWNLYYWGLNPRTFAMPTGKCPLEYYPILTTHYKYLRGCNDYAMHIP
                     LNIQGLGYLAFQSSWTADIYKERIRRGIANGEDLIILGFHAIEEPDNIYGTNCPLREF
                     KEILRYINHLGLEVLPLYEAVEELN"
     gene            complement(1039..2106)
                     /locus_tag="PLB65_05100"
     CDS             complement(1039..2106)
                     /locus_tag="PLB65_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77939.1"
                     /translation="MNRIMRNYHSHRERFSMENKIAKMLVVTLLVCCCVTLSAADVPF
                     VLSWKSINSGAYCPNQAKKHLHWNEFYQIGFGVDSLAYKDLYATLELRSRANFYYNNV
                     EIYNCDFVYNKSNWQIMAGSLPWGYGLPNALYTYALVAADQDQFSYQATRLNRVSLGH
                     YFSNHLLSLAIGGNNHNQSIEVLTYQVIKPFGSLSISEEIRAQDNHWKTPVSISSINS
                     QIDKGNIALNAQIAVSYLPKYENTEQHTTSYGLLAVNYKPNDLTELFLSTEYIEVEPS
                     LNDEQRYHIGISRKIDDFFLNPFYYLDYYDNSSFHRAGLSLDYLFHKNQRVGLVYYLE
                     GSKINKAKHNFAIQAQLGIKA"
     gene            2359..3558
                     /gene="coaBC"
                     /locus_tag="PLB65_05105"
     CDS             2359..3558
                     /gene="coaBC"
                     /locus_tag="PLB65_05105"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOQ77940.1"
                     /translation="MQGKNILLCVSGGISAYKAIDLASRLCKQGFEVKCVLSENATKF
                     VSPLNFSAITHNSVHTSLFTDADPIPHITLATWADLLIVAPVTANILAKAANGIADDL
                     LSTILVAYDKPVLFVPAMNVKMYHSAIVQENIERLLERGHYILQPATGKLACGTEGEG
                     KYPPNEEIIYAIQTYLNYTLDLKGIKVLINAGATVEAIDPMRFLSNRSSGKMGLAIAR
                     ALALRGAEVTLLYGNLQVELPYNIQDKVFCESADTMFRETIKRYPEMDWIICCAAVAD
                     YKPKEINTQKLPKLSQLNLELISTPDILFELGKLKKENQRLIGFAAQSDNLIRSALDK
                     YYRKNLDLICANDIRYAGRDEDEIIIQGKIKGYPPLKEDPNINSVVMKGDKFSLAHNL
                     INIIKEL"
     gene            3555..4298
                     /locus_tag="PLB65_05110"
     CDS             3555..4298
                     /locus_tag="PLB65_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOQ77941.1"
                     /translation="MRQILISGANSQVGSYLAKEYYKEGFQLVLLYHNRNERIAEQLG
                     KEGIFSCSVDLCDATAVQAAISESVKQLKQNSDILIHCAAVRSSDALPLFQTEPFQFT
                     YVLKSNIISAYNVLRYSLPPMQEKRFGRVIMFGSDVSRKGLKNGSAYAASKAAIANMV
                     KSVALELAKDNILINTVSPAPIKTALEEDYSGEYLQFRQRYFADYLTNVPTGKFVTKE
                     EIKKVIDLLISEDLQNLTGEEIFIDGGSK"
     gene            4295..5062
                     /locus_tag="PLB65_05115"
     CDS             4295..5062
                     /locus_tag="PLB65_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOQ77942.1"
                     /translation="MKAKLGLTCLLLVLTLNLIAAAVPFRDGEKLTFTVKYGIVSAGE
                     ASLQARSSVYQGSPVWYLSTTAKTYPFFDKVYKVRDRVESWWDKDTLLPYKFSKNLQE
                     GNYRQHRVHIYNHQEKSSTYQKWSFKKSVFENTEMVIPEGTQDILSAFYLMRTKKLEV
                     GKSVMVNITADGRNMPTEVVVHRKEKQKTIFGNVECLVIEPKLKGEAIFKQSGRILIW
                     LTNDDYKIPVRLESKITIGSFVATLTSAQQVPFKIQL"
     gene            5059..6099
                     /gene="queA"
                     /locus_tag="PLB65_05120"
     CDS             5059..6099
                     /gene="queA"
                     /locus_tag="PLB65_05120"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOQ77943.1"
                     /translation="MIPDLLATNSYDYYLPKELIAQYPAEQRTASRLMHIKRNTGEIA
                     HRQFTDILELIKEGDLVILNSSKVFPARLFAEKENGTKVEILLLYQLNDNKWKCMASP
                     GKRLKQPQYLHFSENLSGFISSGDREGLREIDFTCQINFWEEIQRIGHIPLPPYIERN
                     DTFSDRERYQTVYAKEIGSIAAPTAGFHFDNDILDALRDKGVLFSDVILHIGIGTFRP
                     VKTEYITEHHMHSELATIPESTAEAINIAKKEGRRVICIGTTSTRSVESFWQEGKMQS
                     GSKWTDIFIYPGFQFQVTDALLTNFHLPKSTLLMMISAFGGFETIQKAYRIAVERKYR
                     FFSYGDAMFIED"
     gene            6100..6813
                     /locus_tag="PLB65_05125"
     CDS             6100..6813
                     /locus_tag="PLB65_05125"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOQ77944.1"
                     /translation="MRNYYYYDKLDSTMLEYQRLKEVNDLPLAVRAGTQKDGVGRANR
                     IWLSPPGGLWFTFDYEYNKSLPSFALYMGFCIHQCLESLFEPLQGKLQIKWTNDIIFS
                     ERKLGGILCHYQPIKRTYIIGVGLNTNNEIDSELGKFGATNLKSILGFEVSNSMLCHQ
                     IIKTVEDNCKYMENVVSYLTYCNENLFGRGKLALIEMGGINIEAEILGIDHNGALMVR
                     NNWGELIFVHTGSILEFLD"
     gene            6947..8605
                     /locus_tag="PLB65_05130"
     CDS             6947..8605
                     /locus_tag="PLB65_05130"
                     /inference="COORDINATES: protein motif:HMM:NF024821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ77945.1"
                     /translation="MKKWILIALTAVLLLIGCTANRTVPTTEETKPFSGVVTKVAILP
                     LKTMDSSSRYIQKILTVRDLNYVFAKYPQYKLLNMNEVAEHFKYSGYQDVEDLDIDEM
                     KELADMTGCDILVMGSITSVRSDEFAMAMRFFSVKTEELTQLDFMVNKDKVKRWDALE
                     KSFIAKLDDVVSNEVNKIYNIALNYFANGNYGEAEKNLKLAMGLDPELKDAYYYMGSI
                     YNKQGKTDLAIQNLEMNLAKNPEHTPTLITLIDIYEKTNQTSKRLEAMEKLATINENE
                     DLWLTIGNMYAEQKNVSKAEHALKKAIEINPNFTEAQTRLAFLYYDNNRYDEAITYLE
                     YLFDKFPDNELISNRLAVAYQKANRLDDAISKYETIIKNNPQNTMAYLNAVNLYRLKA
                     SESTDPAFVASINKKAIDTLSALIKQHPDNALAYMNMAAIYLGQNKYQEAETYALQAL
                     KKDPTLYLPYVYLATISQSKGTNDYNHFIDLEKQAAKAVGTKAKTLKNQRDNAKSSAV
                     SNFRKALDYLNNAKVRATSEEAITDINNRIARVNQLITQATSTY"
     gene            9157..9279
                     /locus_tag="PLB65_05135"
     CDS             9157..9279
                     /locus_tag="PLB65_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77946.1"
                     /translation="MDKKVTQRSTYKAFKMKIVEERHPAVPLLTAKLCSYRYML"
     gene            complement(9577..10026)
                     /locus_tag="PLB65_05140"
     CDS             complement(9577..10026)
                     /locus_tag="PLB65_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOQ77947.1"
                     /translation="MKHYFLILIASIICVPILLSGKEETDRIIGYWLTGDKATKIEIY
                     QNEDGTFAGKIIWLQEPNDAKGNPKKDINNPDVKLQQQPLIDLIVLSDFVRENRNKYK
                     NGKYYEPTTGKTYSAKIELINNNTITIRYFIGVPTLGRTDTWIRSSN"
     gene            complement(10042..11337)
                     /gene="purB"
                     /locus_tag="PLB65_05145"
     CDS             complement(10042..11337)
                     /gene="purB"
                     /locus_tag="PLB65_05145"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOQ77948.1"
                     /translation="MITRYSKPEMERIWTQENRYECWLEVELAAAKAMYEKGMIPAED
                     WETINSKADFDSNRIDELEQVTKHDVIAFLTNVAENIGPASRWIHFGMTSSDVLDTAT
                     GMQLKSSGELILTELSRLAEILKLKARQYRNTICIGRSHGIHAEPTSFGLKFALWYEE
                     IQRNIIRLNEAIEEVSVGKFSGAVGNFAHLSPEIEDMACKFLDLKPAKVSTQIIQRDR
                     YAYFLSVLALIASAIEKMALEIRHLQRTEVHEAEENFSKGQKGSSAMPHKRNPIISEQ
                     LCGLSRVLRSNALSAMENNALWHERDISHSSVERIILPDSCILIHYMLNKTCSLIENL
                     VVYPENMKKNLELTKGLVFSQALLLHLVKSGLTREKAYALVQQEAMKSFETNTNFLDN
                     VLANKEITTALAESEIRDIFSYERYLRNVDYIFRRCGIL"
     gene            complement(11340..12071)
                     /locus_tag="PLB65_05150"
     CDS             complement(11340..12071)
                     /locus_tag="PLB65_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77949.1"
                     /translation="MKKTVLVLLTITAIIITACAGPKGISENEKLRLELKKWESFDSQ
                     GVVELSYKGLAIRKMFIASKNGNELRFDIIDGGLMGSKAEPLMSVYVADYVSIRSPFI
                     PMLESLDISQAIPMESLTLSTNVDSLVTHYAQKIIKDKKIELNGVVISFSKNYLLDTV
                     LDSKSNTKIKALYTGNSKLSELYITSNDDISIRLSFDKIEYIKPHIVPLPKAEPSSLE
                     NIIQDLNKISIDSLLRDYLQYRKEK"
     gene            complement(12102..12932)
                     /gene="lgt"
                     /locus_tag="PLB65_05155"
     CDS             complement(12102..12932)
                     /gene="lgt"
                     /locus_tag="PLB65_05155"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HOQ77950.1"
                     /translation="MITYPNIDPNIVSFSIGNLNLHIRWYGLFYVLSFILAFILYKPF
                     LKKRNIKLTKEQYESIIFYMMLGVILGGRLGYILFYNLRYYLSHPIMIFAVWEGGMSF
                     HGGALGVIIAGLIFCHKHKLNFYAMADSAIPIVAIGLGLGRLGNFINAELYGKPTNLP
                     WGMIFPGSDGAVRHPSQLYELILEGIVMFVITYFLLKKLKKEGVVFWSFIGLYGIFRF
                     LIEFVREPDDLDFYTRSGYIFGFMSIGQFLSLLMMIIAIWGFYSIYKTPKKQEQRKTN
                     "
     gene            complement(12929..13714)
                     /locus_tag="PLB65_05160"
     CDS             complement(12929..13714)
                     /locus_tag="PLB65_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOQ77951.1"
                     /translation="MHYIVCVKQVPNTTEIKIDPKTNTLVREGVESILNPFDAYAVEE
                     AVRLKEKYGGTVTAISMGPNQCETTLRETVSLGVDNIILLSDRRFAGADTLATSLTLA
                     AAIRKIGDYTLILTGQQAIDGDTGQVGPGIAAHLKIPQTCYVRKIESFSENSAVVERL
                     LEDGYDRISMQLPAVISVVKEINVPRLPSLRGKKNAKTAVLTVWNCDDLGLNEKETGL
                     NGSPTQVMSIFSPHHEKQVEKFEGNSDEAVELIIEHLSKLTHR"
     gene            complement(13727..14833)
                     /locus_tag="PLB65_05165"
     CDS             complement(13727..14833)
                     /locus_tag="PLB65_05165"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOQ77952.1"
                     /translation="MNVPMLDLKAQYVPVMGRIKEALEGVFAEHQYIMGPPVKEFENK
                     MADYLGIKNAIGCASGTDALVLAIKALEIGEGDEIITTPFTFFATASSISRNNATPVF
                     VDIDPLTFNLDPDKIEAAITEKTKAILPVHLFGQCADMDSIMAIAQKYNLKVIEDNAQ
                     GIGCTWNGKMSCAFGDIGTLSFFPSKNLGAMGDAGMCMTNDDDLAFKLRQLRVHGENP
                     KYYHKWIGLNSRLDTLQAAVLGVKLDFLETWSKGRRANADFYNSHLKNIPGIRIPHID
                     SRAVSIYNQYTLVCEKRDELMAYLKSKGIGCAVYYPLPLHLQECFSSLGYKKGDMPVA
                     EELSLKVLSIPIYPELTDEQKQYVCETITNFYFG"
     gene            complement(14852..>15986)
                     /locus_tag="PLB65_05170"
     CDS             complement(14852..>15986)
                     /locus_tag="PLB65_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HOQ77953.1"
                     /translation="LLSPPKPEKETKDKMGYEKNQGLRSIQIIRNGKTETYDLLKFYR
                     MGDINNNPFLKDGDVVYVPAIKNFVSIGGGINLPGELEFVEGDKISTIIELSLGFTFD
                     ADISKLQLYRYKENRIDYDVLNYDLKANPSLLNLPLQADDRILISCDSEIRTRQRIKI
                     YGQVKNPGEYVIDTNTTLYEVLQQAGGLTKRGNIKTMVYYNENINVDPDPYFEMLMQR
                     SLNEMTPLEYSYLRTNLQQLKGKYSIDPVKMMDSEGKDSNPTLFDGDIIYIPEKINMV
                     WVSGQVKNPGMIPWMEGKDWSYYIQAAGGYTNNRKTGKDRIIRGTSGNWVKPNKKVAI
                     QPGDTIFIPAQTDRSLWTDVKDIVTLTSSVVTIILGIHALTAN"
     assembly_gap    15987..16086
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16087..16280)
                     /locus_tag="PLB65_05175"
     CDS             complement(<16087..16280)
                     /locus_tag="PLB65_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77954.1"
                     /translation="MRIKFILILTLSLSLSLLVATENSTIEGIVPINISLTGYVENPG
                     VYQLTPLNRLSDLLMISKSTA"
     gene            complement(16270..17229)
                     /locus_tag="PLB65_05180"
     CDS             complement(16270..17229)
                     /locus_tag="PLB65_05180"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOQ77955.1"
                     /translation="MRILITGGAGFIGSHLAEKLLEEGNNVYVIDNLSTGRLENIESF
                     KDKPKFHLTIGSVLNRELMDKLISNVDQVYHLAAAVGVKYIIENPLLSLKTNIGGTDN
                     VLELCNKYKAKALIASTSEIYGKSEQVPFSETDDRLLGSTHISRWGYSCSKAIDEFLA
                     LAFYREKKLPVVIVRCFNTVGPRQTGQYGMVLPKFIKAALLNQPLIIYGTGEQTRCFA
                     DVSDVIQAFIKLMNTPECAGEIFNVGTTESISISALAHKVKDMCHSKSPIDYMSYNDA
                     FEEGFEDMMHREPNLSKIKKYIDWEPKLKLDDIITRMIEYYEN"
BASE COUNT         5673 a         3099 c         3018 g         5435 t
ORIGIN      
        1 cctgaccttc accttcggag ggtgccactc tatccagctg agctacaggg acgcataaaa
       61 tactgatcca taaattctgg aaagcagttt tctgtcaatt gagaaagtga gaagcaagaa
      121 tctttatcac tttgagcata atttcagctt agtacaaaaa agaactcgta gaccttatca
      181 gaaatgcaat attagcgatg ttttaatata tgcttattat gattatattt agcagccaag
      241 taaattaagt gccactccta ttattacaag tgcagatcac tcttatctaa ctatctgata
      301 attagcataa ttaattcagc tcttctacag cttcataaag aggtaaaact tccaatccaa
      361 gatgatttat atagcgtaat atttctttaa attctctcaa gggacagttt gttccataaa
      421 tattatccgg ctcttcgata gcatgaaaac ccagaattat taaatcctca ccattagcaa
      481 ttccgcgtct gatacgctct ttgtagatat cagcagtcca agaactttga aaagcaagat
      541 aacctaaacc ctgaatattt agaggaatat gcatagcata atcattacaa cctcgaaggt
      601 acttataatg tgtagttaaa ataggataat actccaaggg acattttcct gtcggcatag
      661 caaaggtgcg gggatttaag ccccaataat acagattcca ataatcttga ttaatagaat
      721 attccacttc atcgtaagtt aattctgcca aatcctcatg tttaagagag tgccctccaa
      781 tttcccaatt ataatcatta tgcaaagatt gaacctgtgc taaattcatt gaatagcgtg
      841 tagacatata aaaagaattc acatagcaag ttcctctata accatagata ttcatttcag
      901 gtaaagcata agtataaact ccaattcttt gatcatcaaa ggttaagcaa ataatcggag
      961 tatttcgttc cgtgggagta aaaaagttgc aagaaccaag cagcaaagat agtgataaga
     1021 ccaataatga aagctttttc atgcctttat tcccaattgt gcctgaatgg caaaattatg
     1081 ttttgccttg tttattttac ttccttccag ataatacact aagcccaccc tctggttttt
     1141 gtggaaaaga taatctagcg ataaaccagc tcggtggaaa gaactattat cgtaatagtc
     1201 aagatagtaa aatggattaa ggaaaaaatc atcaatttta cggctaatgc caatatggta
     1261 acgctgttca tcattaagtg atggttctac ttctatatat tctgtgctaa gaaataattc
     1321 cgttaaatcg ttgggtttgt aatttacagc cagaagacca taagaagtag tatgttgctc
     1381 tgtgttttca tattttggaa gataactaac agcaatttgc gcatttaagg caatgttccc
     1441 cttatcgatt tgagagttta tagaacttat agatacaggg gtcttccaat gattatcctg
     1501 tgcccttatt tcctcactga tagaaagaga gccaaaaggt tttatcactt ggtatgtgag
     1561 aacttctatg gattgattat gattgtttcc tccaatggca agagataaga gatgattaga
     1621 aaaataatgt cccaaactaa ctctgtttaa tcgagttgcc tgataactga actgatcttg
     1681 gtcagctgca acaagagcat atgtataaag ggcgttgggt aaaccgtaac cccaaggaag
     1741 agaacctgcc ataatttgcc aattgctttt attgtaaaca aaatcacaat tataaatctc
     1801 tacattgtta taataaaaat tagctctgct gcgaagctca agagttgcat ataagtcctt
     1861 ataggcaagg gaatcaactc cgaatccaat ttggtaaaat tcattccaat gcaggtgctt
     1921 tttggcttga tttgggcagt atgctcctga atttattgat ttccaactta atacgaaagg
     1981 cacatctgca gctgagagag taacgcaaca acatactaat aaagtaacaa ccagcatttt
     2041 ggctatctta ttttccatag agaaacgctc tctatgcgaa tggtagtttc tcattatgcg
     2101 attcatcttc aatgcaatga ttcctctttt attagtaaaa ctctttataa aaaggttaat
     2161 gcttactgtc aattactttt ttccttgagt tctgaggttt ttatttttga ttatcctgcc
     2221 aatcttcata ttacatagct aagtctccct atataagtta gttagctctc cactattttt
     2281 tttaaaactg tttttatgat tgacataaat accatagata aatttaaagt ataagctaat
     2341 aacatctaaa gaggaaaaat gcaaggcaag aacatcctac tctgtgtaag tggaggcata
     2401 tcagcttaca aagctataga tttagcaagc cgtttatgta aacaaggctt tgaagtgaag
     2461 tgtgttttat ctgaaaacgc tactaaattt gtgtccccac taaatttttc agctattact
     2521 cacaatagtg ttcatacctc tctatttaca gatgcagatc ctattcctca tattacactt
     2581 gctacctggg cagatttatt aattgtggca cctgtaactg ccaatatatt agccaaagca
     2641 gcaaacggaa tagcggatga tttgctttca acaatattag tggcatatga caaaccggtt
     2701 ctttttgttc ctgcaatgaa tgtaaaaatg tatcattcag ctatagttca ggaaaacatc
     2761 gaaagattac ttgaacgggg gcattatatt ttgcaaccag caacaggtaa acttgcttgt
     2821 ggaacagaag gagaaggaaa atatcctcct aatgaagaga taatttatgc aatacagacc
     2881 tatttaaatt atactttaga cctaaaaggg ataaaagttt taataaatgc tggagcaaca
     2941 gttgaagcca ttgatcctat gcgttttcta agtaatcgtt catctggaaa aatggggctt
     3001 gctatagcac gcgctcttgc tttaagaggt gcagaagtaa ctttacttta tggtaatctt
     3061 caagttgagc ttccttacaa catccaagat aaagtatttt gcgaaagtgc agatacaatg
     3121 tttagagaga caatcaagcg ttatcccgaa atggattgga taatttgttg tgcagcggta
     3181 gcagattata aacccaaaga aataaatacc caaaaattac caaaactctc acaactcaat
     3241 ttggaactga tttccactcc ggatatctta tttgagctag gtaaattgaa aaaagagaat
     3301 caacggttaa ttggttttgc tgcacaatcc gataatttaa taagaagtgc cttagacaaa
     3361 tattatagaa aaaatctgga tcttatttgc gcaaacgata ttcgttatgc aggcagggat
     3421 gaggatgaaa ttattattca gggtaagata aaaggttatc ctcccttaaa agaagatcct
     3481 aatataaact ctgtggtgat gaaaggagat aaattttctt tggcacataa tttgataaat
     3541 atcattaaag agctatgaga cagattttaa taagcggtgc taattctcag gtaggaagct
     3601 atctagcaaa ggaatattat aaagaaggat tccagctggt tttattgtat cataaccgca
     3661 atgaacggat tgctgaacaa ttaggtaaag aaggcatatt ttcctgttct gtagatctat
     3721 gtgacgctac agccgttcag gcagctattt cagaatcggt aaagcagtta aagcaaaatt
     3781 ctgatatctt aattcactgt gcggcagttc gcagttcgga tgccttgcca ctatttcaaa
     3841 cagagccgtt tcaatttact tatgtattaa aaagtaatat tatatcagct tacaatgtat
     3901 tgcgctattc tcttcctcca atgcaggaaa agcgatttgg aagagtaata atgtttggca
     3961 gtgatgttag ccgcaaggga cttaaaaatg gcagtgctta tgctgcctcc aaagcagcta
     4021 tagcaaatat ggttaaaagc gtagctctgg aactggctaa agataatatt ttaattaata
     4081 ctgtttcacc tgctccgata aaaacagctt tggaagaaga ttatagtgga gaatatctac
     4141 aatttaggca aagatatttt gccgattatt taactaatgt gcctaccggt aaatttgtaa
     4201 caaaagagga aattaaaaaa gtgatagact tgcttatcag tgaagacctg caaaatctaa
     4261 ctggagaaga gatctttatt gatgggggtt caaagtgaaa gcaaaattag gattaacctg
     4321 tctgcttcta gttttgactc taaatttgat agcagcagct gttcctttta gggatggaga
     4381 aaaattaacc tttactgtca aatatggaat agttagtgca ggagaagctt ctttgcaagc
     4441 tcgcagttct gtttatcaag gttctccggt ttggtatctt tctacaacag ctaaaactta
     4501 cccctttttc gataaagttt acaaagtaag agatagagtt gaatcctggt gggataaaga
     4561 tactttacta ccttacaaat ttagcaaaaa tcttcaggaa ggtaattaca gacagcatcg
     4621 agtgcatata tataatcatc aggaaaagag ttctacatat cagaagtgga gtttcaaaaa
     4681 aagtgttttt gagaacacag aaatggttat tcccgaaggg actcaagata tattaagtgc
     4741 tttctatctg atgcgtacta aaaaattgga agtgggaaaa tctgtaatgg tcaatattac
     4801 agctgatggc agaaatatgc ctacagaagt agtagttcac cgcaaagaga aacaaaagac
     4861 tatctttggc aatgttgagt gtttagttat tgagccaaaa cttaaaggag aagcaatttt
     4921 taagcagagc ggtagaattt taatttggct taccaatgat gattataaaa ttcctgttcg
     4981 actagaaagc aagattacta taggtagctt tgtagctact ttaaccagtg cccaacaggt
     5041 gccttttaaa atccagttat gattcctgat cttttagcaa caaatagtta cgattattat
     5101 ttgcccaagg aattaatcgc tcaatatcct gctgagcaaa gaacggcatc tcgattaatg
     5161 catattaaaa gaaacaccgg ggaaattgct catcgccaat ttactgatat tttggaattg
     5221 attaaggaag gagatctggt aattctcaat agcagcaagg tttttccagc tcgtcttttt
     5281 gccgaaaagg aaaatggtac aaaagtggaa attctgcttt tatatcagct gaatgacaat
     5341 aaatggaaat gtatggcttc accggggaaa agattaaaac aacctcaata cctacacttc
     5401 tctgagaatc tgtctggttt tatcagttct ggagatagag aaggattaag agaaatcgat
     5461 tttacctgcc aaattaactt ttgggaagaa attcagagaa tcgggcatat tcctcttcca
     5521 ccatatatag aacgtaacga tactttttca gatagggaga gatatcaaac cgtatatgca
     5581 aaagaaattg gctctattgc tgctcctaca gcaggatttc attttgataa tgatatttta
     5641 gatgctctcc gagataaagg agtcctcttt agtgatgtta ttcttcatat tgggattggt
     5701 actttccgtc cggtaaaaac agagtatata acagaacacc atatgcattc tgaacttgct
     5761 acaattcctg aaagcactgc agaggcaatt aatatagcta aaaaagaagg aagaagagta
     5821 atttgcatag gaaccaccag tactcggagt gtggaaagtt tctggcagga ggggaaaatg
     5881 caaagtggtt ccaaatggac agatattttt atctatccgg gttttcagtt tcaagtaacc
     5941 gatgcattat taaccaattt ccatcttccc aaatctactt tgttaatgat gatctccgct
     6001 tttggcggtt ttgaaacaat acaaaaagca tacagaatag ccgtagaaag aaaatatagg
     6061 tttttcagtt atggcgatgc tatgtttatc gaggattgaa tgcgaaacta ttattactac
     6121 gataaattag acagcactat gctggaatat cagcgtttga aagaagtaaa cgatctccct
     6181 ctggcagtta gagctggaac tcaaaaagat ggagttggca gagcaaatag aatttggctt
     6241 tctcctcctg gaggtctatg gttcactttc gattatgaat ataataaatc tctaccttct
     6301 tttgccctat atatgggctt ttgtattcat caatgtttgg aatcactatt tgaacccttg
     6361 caaggtaagc tccaaattaa atggactaat gacataattt tctctgaacg aaagttaggt
     6421 ggcattcttt gtcattatca acccatcaaa agaacttata taataggtgt gggattgaat
     6481 accaataatg aaattgattc cgaactgggt aaatttggag caacaaactt aaaaagtatt
     6541 ttgggttttg aagtttccaa tagcatgctg tgtcatcaga tcattaaaac cgtagaagat
     6601 aattgtaaat atatggaaaa tgtggttagc tatctaactt actgtaacga aaatctcttt
     6661 ggaagaggca aattggcttt aatagaaatg ggggggatca atattgaagc agaaatttta
     6721 gggatagatc ataatggtgc tttgatggtt agaaataatt ggggtgaatt aatttttgtc
     6781 catactggct ctattctgga gtttttagat tgaataaaaa agtttgaatg tctatgaaag
     6841 tcgaatataa tgttttatag ctgaataaat ggttttcctt gacagatagt tagaatttaa
     6901 aaatgagtaa aataaattaa tttgaaattg atacagtgga ggaacaatga aaaaatggat
     6961 actgatagcg ttaactgccg tgttactttt aattggctgc acagcgaatc gcacagttcc
     7021 cacaactgaa gaaaccaaac ccttttcggg ggtcgttact aaagtagcca ttctacccct
     7081 aaaaactatg gattcttcaa gccgctatat acaaaagatt ttaaccgttc gtgaccttaa
     7141 ttatgttttt gccaaatatc cgcaatataa gctgcttaat atgaacgaag ttgcagaaca
     7201 ttttaaatat tctggttatc aggatgttga agacctggat atagatgaaa tgaaagaatt
     7261 ggcagatatg accggttgcg atattttagt tatgggtagc atcacttctg tgcgtagcga
     7321 tgaattcgca atggctatgc gttttttcag cgttaaaacc gaagagctta cacagctgga
     7381 ttttatggtt aataaagata aggtaaaacg ctgggatgca ttggagaaat cgtttattgc
     7441 aaaacttgat gatgtggttt ccaacgaagt gaataaaata tataacattg ctttgaatta
     7501 tttcgctaat ggtaattatg gtgaagctga gaaaaatctt aaattagcta tgggactcga
     7561 tcctgagctt aaggatgctt actattatat gggttcaatt tataataaac agggaaaaac
     7621 agatttagca attcaaaatc tagaaatgaa tcttgcaaag aatccggagc atacaccaac
     7681 tttaattacc ctgattgata tctatgagaa aaccaatcaa acttccaaac gcttggaagc
     7741 aatggaaaaa ttggctacaa taaatgagaa tgaagattta tggcttacaa ttgggaatat
     7801 gtatgcagag cagaaaaatg taagtaaagc cgaacatgca ttgaaaaaag caatcgaaat
     7861 taacccgaat tttactgagg cacaaactcg tctggctttt ctatattacg ataacaatcg
     7921 ttacgatgaa gctattactt atctggaata tttatttgat aaatttcctg acaacgaact
     7981 gatttccaac cgccttgccg ttgcctatca aaaagctaat cgattggatg atgctatttc
     8041 caagtatgag acaattatta aaaataaccc ccagaataca atggcttatc taaatgcggt
     8101 taatttatat cgtttgaaag catcagaatc taccgatcct gcatttgttg cttccattaa
     8161 taaaaaagct attgatactt taagtgcttt aataaaacag caccctgata acgccttagc
     8221 ttatatgaat atggctgcta tctacctcgg acagaacaaa taccaggaag cagaaactta
     8281 tgccttacaa gctctgaaaa aagatcctac tctctatttg ccttatgttt atttggcaac
     8341 tatcagccaa agtaaaggaa ctaatgatta taaccacttc atcgatttgg aaaagcaagc
     8401 agccaaagct gttgggacta aagctaaaac tttaaaaaat caacgcgata atgctaaaag
     8461 ctcagccgtt agcaatttca gaaaagctct ggactatcta aacaatgcaa aagtccgtgc
     8521 aacaagtgaa gaagctatca ctgatatcaa taatcgtata gcaagagtta atcaactaat
     8581 tactcaagca acctcaacat attaatgtaa actgtatact acatagaaaa tatcaaaaac
     8641 ccggtttttc agccgggttt ttgctttaag cttgatattt ccccaagttt aaattctcat
     8701 tcattatctt gcataataag tatgataagc aaaacggatg cacttatcta tatgttcttg
     8761 cctgatagct gctatcttgc tcagttaata taactagaac tgttgcacat atcaaattgg
     8821 tgtcccctgt tttttagcca acctttgaga tactatcact tcccatatat agtgaattgt
     8881 tcatcaagat aagaagaata ttaagatcga taatgcgaag ccattgatta ttactaacac
     8941 ccggaagatt tacaacatat aaatagttgg cttgctctaa tccttacttt tcttgctggg
     9001 taacccagat acaacacatc atccacccct tcttcactca tccacaacta taggttgaag
     9061 aggagaggaa gaggagagga ggaggagagg agtacgggtt gtttgtgagg cgtcctaaaa
     9121 aagttgacac aggatccaaa atcaaggaga agaaaaatgg ataaaaaagt aactcaaaga
     9181 agcacatata aagcctttaa aatgaagatt gtggaagaac gacatcctgc agttcctctt
     9241 cttacagcca aattatgcag ttaccgctat atgctataat agcttgaaaa gatcattgca
     9301 cataacaatg aacaaatttg agagttttgg ggaggggaaa atgaaaaaaa gagaaaaatt
     9361 agacctttat tttgtgatta tttctctaat ttagctaaaa atcatcgaga taatgtcctt
     9421 gctcctagtt aatcaatggt catattacta taaatattct gtgtcgattg ctttagggag
     9481 aatggcatcc ggaaattcaa gtcaattaaa ttacatctat tcgtgatttc atcgtgaatc
     9541 tatatggtcg taaatgcatt gattaggttc aattaatcag ttagagctgc gtatccaggt
     9601 atcggttctc ccaagagtag gaacaccgat aaaatagcgt attgtaattg tgttattgtt
     9661 aataagttca attttagcgg aataggtttt ccctgtagtc ggctcgtaat atttcccgtt
     9721 tttatatttg tttctatttt ctctaacaaa atcagatagg acaattaaat caatcagagg
     9781 ttgttgttgc aattttacat caggattatt aatatctttt ttagggtttc cttttgcgtc
     9841 gttaggttct tgcagccaaa ttatcttccc ggcaaaagta ccatcttcat tttggtatat
     9901 ttcaatttta gttgctttat caccggtaag ccaatagccg ataatgcggt ctgtttcttc
     9961 ttttccggaa agcagaatag gaacacatat tatcgaagca atgaggataa gaaaatagtg
    10021 tttcataacc tagctccttt gttacaaaat tccgcagcgc ctaaaaatat aatccacatt
    10081 acgcagatag cgttcataag aaaagatatc tcgaatttca ctttccgcaa gtgcggttgt
    10141 aatttcttta ttggcaagaa cattatccag gaaattagta ttggtttcaa aactcttcat
    10201 agcttcctgt tgaactaagg cataagcttt ttctcgggtt aaaccacttt taacaagatg
    10261 caaaagtaag gcttgagaaa agaccagacc tttggttagt tccaagtttt ttttcatatt
    10321 ttctggataa acaactaaat tttcgattag cgaacatgtt ttgtttaaca tataatggat
    10381 caaaatgcag gaatcaggta aaattatgcg ttctacactt gaatgagaaa tatcgcgttc
    10441 atgccataag gcattatttt ccattgcgct taaagcattg gaacgcaaaa ctcttgatag
    10501 accacaaagt tgttcactaa taataggatt gcgtttatga ggcattgctg aagagccctt
    10561 ttggcctttg gagaaatttt cttctgcttc atgaacttcg gtcctttgta gatgtctgat
    10621 ttccagagcc attttctcta ttgcagaagc aataagagct aaaacagaaa ggaaatatgc
    10681 atatctatct ctttgaatta tctgagttga tactttggca ggtttgagat ccaaaaactt
    10741 gcaagccata tcttcaattt cagggctaag atgagcaaaa tttcctactg cgcctgaaaa
    10801 tttacctacg cttacttctt caatagcttc atttaaacgg attatattcc gctgaatttc
    10861 ttcgtaccaa agagcaaact tgagtccgaa agaagtgggc tctgcatgaa ttccatgaga
    10921 acgtccgata caaattgtat ttctatattg tctggctttc aactttaaaa tctctgccaa
    10981 acgactgagt tccgttaaaa ttaattctcc agagcttttt aactgcattc ccgtggctgt
    11041 atctagaaca tctgaagagg tcattccaaa atgaatccac cgcgatgcgg gacctatatt
    11101 ttcggctaca ttggttaaaa aagcgataac atcatgctta gtaacctgct ccagttcatc
    11161 aattcggtta gaatcaaaat ctgccttaga attgatagtt tcccaatcct ccgcaggaat
    11221 catacccttt tcatacattg cttttgcggc agcaagttct acttccagcc aacattcata
    11281 acgattttct tgagtccaaa tacgttccat ttcgggttta ctgtagcggg ttatcatttt
    11341 tatttctcct ttctatattg caggtaatcc ctcagcaacg aatcaatgct tatcttattt
    11401 aaatcctgta ttatattctc cagagaggaa ggttcagctt tgggtaaagg aacaatatga
    11461 ggtttgatgt attctatttt atcaaagctt aatctaatgc tgatatcatc attgctagta
    11521 atatataatt cagaaagctt actattacca gtgtaaagtg cttttatttt tgtattgctt
    11581 ttactatcta acacagtatc caaaagatag ttcttgctga aagaaattac cactccgttt
    11641 aattcaattt tcttatcctt aataattttt tgtgcatagt gggtgacgag agaatctaca
    11701 ttagtagaaa gggttaaact ctccatagga attgcttgcg agatatccaa agattccaac
    11761 ataggaataa agggggatct aatagaaaca taatccgcca catatacact catcaagggt
    11821 tctgccttag aacccataag gcctccatcg ataatatcaa aacggagctc attgccattt
    11881 ttggaagcga tgaacatttt ccggatagca agtcctttat aggatagctc aactactccc
    11941 tgactatcaa aactctccca ttttttcaat tccaggcgta atttttcatt ttcactaatg
    12001 cccttgggtc cagcacaggc agtaataata atagctgtaa tggttaagag aacaagaact
    12061 gttttcttca ttaccaacct cgttttccta atataaaaga gttaattagt tttcctttgt
    12121 tcctgttttt taggtgtttt ataaatacta taaaaacccc agatggcaat tatcatcatt
    12181 aataagctta gaaattggcc aatagacata aacccaaaaa tatagccaga acgagtataa
    12241 aaatctaagt catcaggttc gcgcacaaat tctattaaaa atcgaaagat tccatataaa
    12301 cctataaagc tccaaaatac taccccttct ttctttagct tttttaaaag aaaataagtg
    12361 atcacaaaca taacaatgcc ttccaaaata agttcataga gctgagaagg atgtcttaca
    12421 gcaccgtctg aacctggaaa tatcattccc caaggtaaat tcgtgggctt cccatacaat
    12481 tctgcgttaa taaaattacc caaacgccct aatcctaaac caatagccac aataggaatt
    12541 gctgaatctg ccattgcata gaaattcagc ttatgcttat ggcaaaagat aagcccagct
    12601 ataataactc ctaaagctcc tccatgaaaa ctcatccctc cttcccaaac agcaaaaatc
    12661 attataggat gagaaagata atagcgcagg ttataaaaaa gtatataacc aagtcgccca
    12721 cctaaaataa ctcctaacat catataaaaa atgatggatt catattgctc tttagttagt
    12781 ttaatattcc gctttttcaa aaatggctta tataaaatga aagccaatat aaagcttaga
    12841 acataaaaga gaccatacca gcgaatatga agattgagat ttcctatgct aaaactaaca
    12901 atattggggt ctatatttgg atatgtaatc atctgtgggt tagttttgaa agatgttcta
    12961 ttattaattc aacagcttca tcactgttac cttcaaactt ttccacctgc ttttcatgat
    13021 ggggagaaaa aatactcata acctgtgttg gagaaccatt taaaccagtt tctttttcgt
    13081 tcaatccaag atcatcacaa ttccaaacag taagaacagc agttttggca tttttctttc
    13141 cacgcaaaga aggcaagcgg gggacattga tttccttaac aacagaaatc actgctggaa
    13201 gctgcatcga aatacgatcg taaccatctt ccagtaagcg ttctacaact gctgaatttt
    13261 cagaaaacga ttcaattttg cgaacatagc aagtttgagg aatttttaaa tgagctgcaa
    13321 ttccaggacc tacttgtcca gtatctccat caatagcttg ttgccctgtt aaaataagag
    13381 tataatctcc aatttttctt atagcagcag ccaaagttaa actggtagca agtgtatcag
    13441 ctcctgcaaa acgacgatct gacaaaagaa taatattatc cactcctaaa gaaacagttt
    13501 cccgcagtgt agtttcacat tggttaggac ccatactgat ggctgtaact gttccgccgt
    13561 atttctcttt taaacgaact gcttcttcaa cggcatatgc gtcgaaagga tttaaaatac
    13621 tttctacacc ttctctgacc aaagtattgg tcttggggtc aatcttaatt tctgtagtat
    13681 tgggaacttg ttttacacac acaatataat gcatttttat tttcctttaa ccaaaataaa
    13741 agttagttat tgtttcacaa acatattgct tttgttcatc tgtgagctcc ggataaatag
    13801 ggatggaaag aactttaaga gaaagctctt cagccacagg catatctcct tttttataac
    13861 caagagaact aaaacactcc tgtaaatgca aaggaagagg ataatagaca gcacacccta
    13921 tgcctttgga tttcaaatag gccatcagct catctctttt ttcacagacg agggtatatt
    13981 gattatatat actcaccgct cgagaatcta tatgaggaat ccttattccg ggaatattct
    14041 tgaggtgtga attgtaaaaa tctgcattag ctcggcgacc cttgctccag gtttcaagaa
    14101 aatccaattt tacacccaaa acagctgcct gcaaggtatc taaacgactg tttaaaccaa
    14161 tccatttatg ataatactta ggattttctc catgaaccct taattgacgc aatttaaaag
    14221 ctaaatcatc atcattggtc atacacattc cggcatcacc catcgcacca agatttttag
    14281 aagggaagaa agaaagagtt ccaatatcgc caaaagcaca actcattttt ccgttccaag
    14341 tacatccaat accctgagcg ttatcttcaa taacttttaa attatatttt tgggcaatag
    14401 ccataataga atccatatcg gcacattgac caaataaatg aactggtaga atggcttttg
    14461 ttttttcggt tatagccgct tctattttat cgggatcaag attaaaagtt agcggatcga
    14521 tatctacaaa aaccggggtt gcattattac gcgatatgga agaagcagtt gcaaaaaaag
    14581 tgaaaggagt agtaataatt tcatcacctt caccaatttc caatgcttta atagctaaaa
    14641 caagggcatc tgttccagaa gcacaaccaa tggcattctt aatccctaag taatcagcca
    14701 ttttattctc aaattcctta acaggtggcc ccattatata ttgatgctct gcaaagacac
    14761 cttccagtgc ttctttaatt cttcccatta caggtacata ttgtgcttta agatccaaca
    14821 taggaacatt cattattgtt tcatctcctt cttaatttgc tgtaagtgca tgaattccaa
    14881 gaataattgt cacaactgat gaagttagcg ttacaatgtc ctttacatca gtccataaag
    14941 aacgatcagt ttgagcagga ataaaaatag tatctcctgg ttgtatagca acttttttat
    15001 taggtttaac ccaatttccg ctggtaccac gaatgatacg atctttgcct gttttgcgat
    15061 tattcgtgta acctccagca gcttgaatat aataactcca atccttacct tccatccaag
    15121 gtatcatacc tggattttta acttgtccac taacccaaac catattgatt ttttctggga
    15181 tgtaaataat atctccgtcg aaaagagtag gatttgaatc ctttccttca ctgtccatca
    15241 ttttaacggg gtctatactg tattttcctt tcagttgctg aagattggtt ctcaaatatg
    15301 aatattctaa aggggtcatt tcattcaagc ttctttgcat cagcatttca aaataaggat
    15361 caggatcaac attaatattt tcattataat agaccattgt ttttatattg ccccgctttg
    15421 ttaaaccacc tgcttgctgg agaacttcgt aaagagttgt attggtatca ataacatatt
    15481 caccgggatt tttaacctga ccgtatatct taattctttg gcgggtccta atttctgaat
    15541 cgcaagatat tagaatacga tcatcagctt gtaagggtaa gtttaataaa gaagggttgg
    15601 ctttaagatc atagtttaga acatcatagt caatacggtt ttctttataa cgatagagct
    15661 gaagtttaga aatatcagca tcaaaagtga aacctaagga aagttcgatt attgtgctaa
    15721 ttttatcgcc ttctacaaat tccagctcac ctggaagatt aatacctccg ccgatggaaa
    15781 caaaattttt gatggctgga acataaacca catctccatc ttttagaaaa gggttattat
    15841 ttatatctcc catacgatag aatttcagca aatcataggt ttcagtttta ccgttcctga
    15901 ttatttgaat gcttcgtaaa ccttgatttt tctcgtatcc cattttatct tttgtttctt
    15961 tctcaggttt aggtggagat aaaagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16081 nnnnnngcag tagatttact tatcattaaa aggtctgaaa gtcggtttaa aggagtaagt
    16141 tgataaaccc ccggattttc tacatagcca gttaaactta tattaatagg aacaatccct
    16201 tcaatggtag aattctcagt tgctacaagt aaagatagag ataatgaaag agttaagata
    16261 agaatgaact taattctcat aatattctat catcctggtt attatatcgt ccaatttaag
    16321 ttttggctcc cagtcgatat atttctttat ttttgataaa ttcggctctc tatgcatcat
    16381 atcctcaaaa ccttcctcaa aagcatcatt ataactcatg taatctatag gagatttact
    16441 atgacacata tctttaacct tatgggcaag agcagatata ctgatagact ctgtggttcc
    16501 tacattaaat atttcacctg cgcattctgg tgtattcatc aactttataa atgcttgaat
    16561 aacatcagaa acatcagcaa aacatcgtgt ttgttctcct gtaccataaa tgattagggg
    16621 ttgatttaaa agagctgctt ttataaattt gggtaaaacc attccatact gaccagtttg
    16681 tctggggcct actgtattaa aacaacggac aataactacc ggtaattttt tttctctata
    16741 gaaagcaaga gccaaaaatt catctattgc cttactacag ctgtaacccc atctactgat
    16801 atgagttgaa cccagcaaac gatcatcggt ttcagaaaaa ggaacctgtt cacttttacc
    16861 gtatatttca cttgtggaag cgataagtgc tttagcttta tatttattgc aaagctccaa
    16921 cacattgtct gttccaccga tattggtctt tagtgataag agtggatttt caataatgta
    16981 ttttacacct accgcagcgg ctagatgata tacttgatca acattactaa tgagtttatc
    17041 catcagttcg cgattcaaga cactaccaat agttaaatga aatttgggct tatctttgaa
    17101 ggactcgata ttttccaagc gccctgttga gaggttatct attacataaa cattgttccc
    17161 ctcttctaat agcttttctg ctaaatgcga gccaataaat ccggctccgc cagtaatcag
    17221 aattctcatt tttactccat ctttctatag ctcaaattgt accatatatt ttaatgttag
    17281 gcatattgat ttgatactcg ttattttgtc aatgagaaag tagtt
//
LOCUS       DAOSTH010000032        16794 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3526_length_16794_cov_10.601290, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000032 DAOSTH010000000
VERSION     DAOSTH010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 16794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16794
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3526_length_16794_cov_10.601290"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..1893)
                     /locus_tag="PLB65_05185"
     CDS             complement(373..1893)
                     /locus_tag="PLB65_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77956.1"
                     /translation="MDIIVEGISSAPYVEITANLQPFFALPGTSSEIQTYQIIATNLT
                     GNLIVTAPQYFYLRLFDTDSFASSLELIPRNGNFVKQIQVVFQPSVSGSYTGNISHSG
                     GGIIPQDIEVSGSTIFPEPTNYPVAFTTAGVTYYQAYLNWIDSTGTSLPNSYLIKGSE
                     VSLADIADPVDGIPENDAELIKNVSYSVQPQLFYGLKENQTYYFKIFPYTNSGETINY
                     KVDANAPSVQFTTSTGPVGSVLLPGDLAFVEYATDSPDRFSFVLLKDIQENTKMYFTD
                     KAYNGTAFLANEALYEWRGVGRSYAKGEVIHLVEGQTFINEGIHSPGVEGLSNDGDQI
                     IAFQGTLDAPSFISAISSTGWLTEGTVNNNSSYLPSTLTLGVNALGFATEVDDGVYNG
                     ATTGSVAEIRTAINNPENWTRAGNLNNISFPVWNFVLNALVMSETVITQISSTTIRIS
                     WQQVSGATHYNVYKSSLPYASFTQDWVLAADNITGLFWETTEITSPRQFYRIIAVN"
     gene            complement(1850..2449)
                     /locus_tag="PLB65_05190"
     CDS             complement(1850..2449)
                     /locus_tag="PLB65_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77957.1"
                     /translation="MKKILLIAFILMGAALNATVISAWTFENTLIPSTGSGSVSLIGG
                     VTDDGFNTGYNGGLAWSTTSYPAQGTNNTTAGIVINVSTANFQNITISWALRHSNKSA
                     NKAVLFYTLDRTVSEPVWIQADIYNATSGDSWFPNSFNASSITGMNNNPNLAFKLVSA
                     FGNTENTLYMPSNPSSTYDGGKWRFDGHNCRRNFKCSLC"
     gene            complement(2459..5566)
                     /locus_tag="PLB65_05195"
     CDS             complement(2459..5566)
                     /locus_tag="PLB65_05195"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOQ77958.1"
                     /translation="MKKAYLVLCLILSFAFSMRAQTDFYDMSTVNTIQLFFTQSNWDQ
                     ILDQLYAAGEDRLIGTAIINGVTYDSVGVRYKGNSSYSANRNKNPFNIKLDHIIEDQL
                     IDGKYGTLKLANGFCDPSFVRETLSYEIARKYMPACKANYANVWVNNVLIGVYTSVED
                     VDSSFMREHFHTSGMPRFKCETNTMSPVTVWGYLGADSTAYMQYYGLESDYGWSTLIN
                     FTNTLQNNYANIDQVMNVDQNIWMCAYDNLLVNLDSPINVFHNFYLFGDADNRINPLL
                     WDLNMSFGGFQGGSVSSMQNLDPLRNSTSNTFLLLKNVLINPRYKKMYIAHMRTMIEE
                     NFSNGWYATRAAELQSVCGPSVQNDPNYFYTYANFQANLNNQVGGGSGGPGGGQTVPG
                     ITQLMNARATYLLSNTNFAGTVPTMTAQSYSPENPEPSSTLQFTATFANATYAQLGIR
                     QNIASKFTYYQMFDDGAHSDGAANDGIYGISVPISYGDINYYFWAENSSQGMFFPARA
                     EHEYFTIAVSSFTGELFINEIMAKNASFADPNGDFDDWVEIYNPNNYAVDLGGMYMTD
                     SHYSNGISAWTQIPTTNPDVTTIPAHSYKIVWFDEDLDQGPLHINDKLGGGADAVYLI
                     DSDGITLVDSYIWTEATDLNVDDRSIGRLPDGTDNWVLFGAGQTNPCTPGASNQGTVN
                     TLPVIENIVYNPLITDENSIITVSASVSDSDGSINSVQLLYGLSDWTLNTVTMSLTEN
                     SYTAQIGPFALDSIIKYRIQATDNVSGVTQSPVYTIVIGYSAPILYINELMPSNSTTV
                     MDENSEYEDWVEIYNPNNFEVDLAGYYFTDDHYPDNGTSLTQIPTGFTETIIPASGYK
                     IIWFDEDLDQGSLHINTKLSATADAVYLIAPDMLTVIDHIAWTEDLALASDISYGRYP
                     DGSENWVKFGTDFENPVTPGTQNYPTGNSDDIITPVVMRFEVWPNPVRDALNINLKGA
                     NDNYRVKVYNLKGQLVTENVMASDGKNQWDLRDKNGNRISSGIYLIRTQFAGKQFSKK
                     ICIIQ"
     gene            complement(5651..7648)
                     /gene="htpG"
                     /locus_tag="PLB65_05200"
     CDS             complement(5651..7648)
                     /gene="htpG"
                     /locus_tag="PLB65_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOQ77959.1"
                     /translation="MADKKTKEKGSLSIHTENIFPIIKKWLYSQHDIFLRELVSNSVD
                     AISKRKYADPNFKEEEMKVEVKLDKGKKTIQVIDSGIGMTAEEIKKYINQIAFSGAED
                     FINKYKDVQNNMIGHFGLGFYSAFMVADKVTIDSLSCTEGSEAAFWECDGSTEYLMSK
                     GKRKEIGTTITIYLNEESAEYANEGKIREILDRYCNFMPYPIMFEGKQVNQKEALWNR
                     KPSDVKDEEYIEFYKNVFHDYMDPVFWIHLNVDFPFNLKGILYFPKLRNEPDFFKGEV
                     KLYCNNVFVADNLEDLIPEFLLLLKGGIDIPDIPLNVSRSFLQNDAQVKKISQYIVKK
                     VADHFNNTFKEDRKKYESYWEDINAFIKFGLLKEDDFFEAMKDIIIFKTASGDYVTIE
                     EYKARNKSEGDKTKIWYAASEDTQVSYLNLMKEQGIEVVFQNSPLDTHLYQQLENKLG
                     NIEFMRIDSEVNDLLVNKEGVASDSEDKLKELFYTALGQKVEASFSKESYADFLKKYP
                     NAATILSPYLIQNDEQTIIKPYELPDNVRKEIGPEAMKALEDNAFTEIKVEVKSLKSP
                     EIPSMIVFSEYMRRWHDMDMLSHQNASSDMLKYHTLVVNRDNPIIKKILAYAEQGKDD
                     VVKTLCSYIHDLSLLEQKPFSGKELKSFINKANQVLSYLSE"
     gene            7877..11797
                     /locus_tag="PLB65_05205"
     CDS             7877..11797
                     /locus_tag="PLB65_05205"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77960.1"
                     /translation="MKRNLIALSFLILFIPLVAVIQETASLKHFLYGHEPNCAYDNWI
                     SHLAEGVVIQGYNTYAPYDRQTNGFGNFVIATTFQLSSWGNIVDLFLEGMLDEAQSAI
                     DASGFPYQVVIFNDTDTNITYRMLREIPDSSYVDQNGTEDDYDDEIGAFAYGWGLYIY
                     NPEGTRPIIVTAPHPCDDFPTPAFALESFKIMDAQFLLINGAGREVKWTNSGSYTNTK
                     SLSDPTRVANHPFNTCYKKFVDKLRIVFNCREFSPQIHSYDWNYHAGYPNVQISAGNS
                     RLCPNLPIRDLSSLKLDMINQGHHIMVPANTVGFHSEVELNDFYSVYYDTYPFIFDDG
                     EHSYEVNNYIDLPAYSQNYQMLYSLENWTDYDVYDPFLHVEMDELPNSYELTENTYRW
                     FYGWNEAQQRWNFDHLFDNFLQYYLRWVYDLNSVLNTMFAMNDGLNPPTPTGFAIHNQ
                     SLYSVTLQWNKVDCYDFDTFEILYSTDPIGTNNYQVYNRSNNAMLASPYCESITVPGL
                     SSSSSYFFKIRTKDKNGNYSDLSNQITTIPAPANVYTFTAHGMDNAVRLYWGVSGQNN
                     NQGFSVYRKNSTQTDYSLIDSYLTNSSLNNPTASSFTYWDYDVANGQDWEYIISCTNT
                     SNQEFFYNYPATAAVRAIHSLYIRNNTATLADTISFAQNSFASDAQDAFYDVTKGSPS
                     GSNYVWAAFWEQYWGNNGTTLSREVKGGYNTALDIKTWAIRIRSTELNTPLYISASDN
                     FNRAEKLYLYDSYTGTWHNLFSGPYQFTVPNSNVRTMTLYWGNLQPKVVHSTQSNQLY
                     QGGNNIPLQWSCQNNFLVDHLDLYIKNETDSLFLVANVPGNQTNFTYNVPQNLDMQSA
                     HFYIDCHAVDGLMQTYRSSYTFAIVPRMVLHSNEAGWQTRSHIWPDLTPTVENIFGTG
                     ATAFIAGDNNTWVECDDFLFGIAYWVNAPDMNFFSSTADICASGIDSFPLEPGWNFVA
                     NPHYCRYPIHSLRFLVGNNLFLYSEMIAQHLISRAVYVYRNGRFQAVDVIEPFESFFI
                     KYYCDQSLNTYLSFYPYFEAPEITPPDNFWQFQVAVTCPASDTDEFILGTNPIATDGY
                     DFYVDLPAAPVKPFPSISAYLTREAPEDISFTDKKLSCEFREVFSPTVDQEKIWHFKF
                     TCSSVEPIEFDLSDINLPNDYTIRIFLGENIYTYRQLTHFTYVPAEPGTYEGLIKVTN
                     YPVSVTDLIQKPVSALAVYPNPFNPTTTIAFSTSQTQVVEVSLYNLKGQKVRTLHKGI
                     LKSGEHKLIWDGKDNTGRTVSSGVYFTRIETGKQSFTRKLLLMK"
     gene            12046..12498
                     /locus_tag="PLB65_05210"
     CDS             12046..12498
                     /locus_tag="PLB65_05210"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOQ77961.1"
                     /translation="MGNQTMTLAEQENINRIANQKHFLKEIDLIIDWTKINKVLSKVE
                     IRRESVAGSDTYSAEVMFRILLIQSWYQLSDYQLEEQLTYNLMFLYFSHLSLENPIPD
                     HSTISRWRSRFSEKNIFDELLEEINRQLVMHNIEVHEGVIVDATLVES"
     gene            complement(13201..13707)
                     /locus_tag="PLB65_05215"
     CDS             complement(13201..13707)
                     /locus_tag="PLB65_05215"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOQ77962.1"
                     /translation="MVGFDLNRVIGKNGKLPWDIPEDLTRFKKITTGYPVIMGKTTWK
                     SIGKALPGRQNIVISKDVSLQLEGAIVVHSIEEALKLVDGQEAFVIGGAQIYKQFLPL
                     ADKLFITMVHSKFDGDTFFPPYNEDEWLLKEKKDIISSTGYPLSFLTYTRKDHFQSEK
                     VNSGKVEK"
     gene            complement(13719..14456)
                     /locus_tag="PLB65_05220"
     CDS             complement(13719..14456)
                     /locus_tag="PLB65_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ77963.1"
                     /translation="MEPIISVKNLVAKYGDNIVLDNISVDIFPGEITVILGGSGCGKT
                     TLLKNILRLVEPFSGSVKIWGEEVLDLDDIQMANILKKIGMLFQSGALLNSISVYDNI
                     SIPLELHTDLSQNLIDRIIKVKLNLVNLSEALYKFPSELSGGMKKRAALARAIALDPQ
                     ILFCDEPSAGLDPLTAASLDELILELKKQLKMTIVVVTHELASIHRIADKIIFLDSGK
                     MLFNGTLEDAKNAGIPQIDTFFKVGKF"
     gene            complement(14459..15535)
                     /locus_tag="PLB65_05225"
     CDS             complement(14459..15535)
                     /locus_tag="PLB65_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family lipid ABC transporter permease
                     subunit"
                     /protein_id="HOQ77964.1"
                     /translation="MPCRIVNNTLYLEGNLTKETVPEVLYRINTLLKNQSPNTIDLSG
                     ISNLDSAGVAFLDELHNRLAAEGLLMFQGVQPQIQEVIDTFTSLKIETPTPARKMGFF
                     EKIGDSVVLAAQNFYQVMMLASEVFYWSLIGIFNRKGQRKGSFIIQCSLLGSQALPIL
                     ALLSFIVGFILALQASVQLANFGANVFIADLMAFSMVREIAPLITAIIVAGRSGSAIA
                     SEIATMQVTEEIDALKIMALSPVRYVVVPKFHAITVVMPILVMFSILVSEIGGGIIAI
                     DYLDLSVYTFVQRSIDVLSMKDIVTSFGKSIVFAWTIVVIGAYCGFEVKGGAEGVGKA
                     TTSSVVASIFAVIIWDAIFSLLYL"
     gene            complement(15544..>16794)
                     /locus_tag="PLB65_05230"
     CDS             complement(15544..>16794)
                     /locus_tag="PLB65_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77965.1"
                     /translation="KPNWGELKIVIMDESKSRVRMGYDLWKKDAEGEGYKIYSGGMVS
                     MGEDEVGAVDKLWILPPGSYLVKLGGGSWSDLKDFATVALAEGDKKTLTMIVDPAAEG
                     NVLIGAGVFADEDIGLGSKRIHRGAIHGNISLSSNNNVDKKKPTSSVSLAGQFDNSID
                     THELIKPFHFTTRSIYDLGLNKTSNQDLIFSPDDYSLKSVLMLYPMEKKKFLRNFAFY
                     GRMNINTHFFDETTYFSENKNIILQNAEGDICSTLLDQPDLKTKIAFYPLRLKEGTGL
                     TYQINFSPRAWTSLRVGYGWLQDYNTSSYVFDKTIVDTQTGLSYDRYIEEPNSSSKGI
                     ESTIIFSALNLLKFVSINSTLDVLFRMGVPDHTYRLENENRISFKLFRNISLDVKFNT
                     FYDEAEKPWMVYDYTTFLRLSLFY"
BASE COUNT         5189 a         3339 c         2970 g         5296 t
ORIGIN      
        1 cagaatatcg gaagcaagct tcgctttttg ctcttacctc caagcgtaaa tcaagcgttt
       61 atcatccctt aatcaagcgt taattattaa cgcttgatta agggatgata aacgcttaat
      121 taatattctc atgtaaggaa gaaattagac aaaaataggg ggattttatg tcgtctttaa
      181 gtcaaaatcg gcatattcta tgagattatt aatgacctta ttcaaagata ggaaaaaaga
      241 atgaaattgt atggaagatc gcgagcaaag ccgattttaa ctcaaagggc gacacgatta
      301 tagcgatggt ggcgtaaata atctgccagc tggaaggaca taccagccgg caaagttaga
      361 taaatgtata aatcagttaa ctgctataat gcgatagaac tgtctcggac tagtaatttc
      421 ggtcgtttcc caaaatagtc cagtgatatt atccgctgcc aaaacccagt cctgagtaaa
      481 actggcatac ggcaaggatg acttatagac attataatgt gtagcgccgg acacctgttg
      541 ccaggaaatt ctaatcgtag tgctactgat ttgggtaatc accgtttcgg acataacaag
      601 tgcatttaga acgaaattcc aaactgggaa gcttatattg ttcaagttgc ccgctctggt
      661 ccagttctct ggattattaa tggcagttct tatttcagca acggaacctg ttgtagcgcc
      721 attatagaca ccatcatcca cttctgtggc aaagcccaaa gcatttactc ccaaagttaa
      781 agtagaggga aggtaagaac tattgttatt aacagtgcct tcggtcagcc aacccgtgga
      841 agaaattgca gatatgaacg aaggtgcatc aagggttccc tggaaggcaa taatctgatc
      901 gccatcattg gaaagacctt ctacgccggg actatggata ccttcattga taaaggtctg
      961 cccttcaacc agatgaataa cttctccttt agcataagag cgtccaactc ctctccattc
     1021 ataaagtgct tcatttgcca gaaaagcggt tccattatat gctttatcgg taaagtacat
     1081 cttggtattt tcctgaatat cttttaacag cacgaaactg aatcggtcag gagaatcggt
     1141 agcatattct acaaaagcca aatctcccgg tagtaaaaca gaaccaacgg gtcctgtaga
     1201 agttgtaaat tgaacagaag gtgcattagc atcaactttg taattgatgg tttcgccgct
     1261 attagtatag ggaaagattt tgaaataata ggtctgattt tcttttagtc cataaaaaag
     1321 ctggggctga acgctataac ttacattttt aataagctcc gcatcgtttt caggaatgcc
     1381 atccacaggg tctgcaatat ctgctaaact tacttcacta cccttaatta aatagctgtt
     1441 aggaagagat gttcctgtag aatctatcca gttcagatat gcctgatagt aagtaacacc
     1501 agcagtagta aaggcaacgg gataatttgt cggttcaggg aaaatggtag aaccgcttac
     1561 ttcaatgtct tgaggaataa tcccgccccc gctatggcta atattgccgg tataacttcc
     1621 actgacgctt ggttgaaaaa ccacttgtat ctgtttaaca aagttaccat tacgaggaat
     1681 aagctctaaa gaagaagcga aggaatcggt atcaaacaaa cgcaggtaaa aatattgagg
     1741 cgccgtaaca attaaattcc cggttaagtt agtagcaatt atctgatagg tctgaatctc
     1801 agaagaagtt cccggcaagg caaaaaaagg ttgtaaattt gcagtgattt caacataagg
     1861 agcacttgaa attccttcta caattatgtc catcaaaacg ccacttccct ccatcataag
     1921 tactactggg attggaaggc atatagagag tgttttctgt attgccaaaa gcggaaacaa
     1981 gcttaaaagc caggttagga ttgttattca tccctgttat ggaactggca ttaaaagaat
     2041 tgggaaacca gctatcaccg ctggtagcat tgtatatgtc tgcctgaatc catacaggtt
     2101 ctgacactgt tctatccaga gtgtaaaaaa gcacagcttt atttgcggat ttattgctgt
     2161 gtcgaagcgc ccaactaata gttatatttt gaaaattggc ggtggaaaca ttgataacta
     2221 ttcccgctgt cgtgttattc gtcccctgag caggataact tgttgtgctc caagccaatc
     2281 caccattata accagtatta aaaccgtcat ctgttacccc tccgattaat gaaacgcttc
     2341 cactgccggt agatggaata agagtatttt caaatgtcca agcagaaata acggtagcat
     2401 ttagtgcagc ccccatcaat atgaatgcta ttaacaaaat ttttttcatt attgctcctt
     2461 actggatgat gcagatcttt ttgctgaatt gtttgccggc aaattgagtt cggattaagt
     2521 atataccact gctaatgcga ttgccgtttt tatcgcgtaa atcccattga tttttaccgt
     2581 ctgaagccat tacattctcg gtaactaact gccctttaag attatacact tttacgcggt
     2641 agttatcgtt ggcaccttta agattgatat ttagcgcatc tctaaccgga ttgggccata
     2701 cttcaaaacg cataacaacc ggagtaataa tatcatcact gttgccagta ggataatttt
     2761 gcgttcctgg cgtaacagga ttctcaaaat cagttccaaa cttaacccaa ttttcactgc
     2821 catcgggata tcttccataa gatatatcac tggctaacgc taaatcctct gtccaagcaa
     2881 tatgatctat cactgttaac atatcgggtg ctattaaata aaccgcatcc gcagtagcgc
     2941 ttaatttagt attgatatgc aaagaaccct ggtcaaggtc ttcatcaaac cagataattt
     3001 tataaccgct agcaggtata attgtttcgg taaatcccgt aggaatctga gttaaagaag
     3061 tgccgttatc gggataatga tcatctgtaa aataatagcc cgctaaatca acttcaaaat
     3121 tattgggatt ataaatttct acccagtcct catattcgct gttttcatcc ataacagttg
     3181 tagaattgga aggcattaat tcgttgatat acaaaatggg agcagaataa ccaattacaa
     3241 ttgtatatac tggcgattga gttactcctg aaacattatc cgttgcttga atccggtatt
     3301 tgatgataga atccaaagca aaaggtccga tttgagcagt gtagctgttt tcggttaaac
     3361 tcattgtaac ggtgttcaaa gtccagtccg aaaggccata caataattga acgctattta
     3421 tgctcccatc gctgtcacta acactggcag atacggttat aatgctgttt tcatctgtga
     3481 taagtgggtt ataaacgata ttttcaataa cgggaagtgt gtttacagtt ccttgattgg
     3541 aagcaccagg agtgcaagga ttggtttgcc ctgctccaaa taaaacccag ttatcggttc
     3601 catctggtaa tctgccaatt gatctatcat caacatttaa atcggttgcc tcagtccaaa
     3661 tatagctatc aacaagagta atgccatcgc tatcaattaa ataaacagca tctgccccac
     3721 cccctaattt atcatttata tgtaaaggac cctgatcgag gtcttcatca aaccacacaa
     3781 ttttatagct atgcgccggg atagttgtaa catcaggatt agttgtcgga atttgagtcc
     3841 aggcacttat cccgttgctg taatgactat cggtcatata cattccacct aaatctacag
     3901 cataattatt gggattataa atttccaccc aatcgtcaaa atcgccgttg ggatctgcaa
     3961 aagaggcatt ttttgccata atttcattga tgaataactc tcccgtgaaa ctggaaacag
     4021 caatagtaaa atattcgtgt tccgctctgg caggaaagaa cataccctga gaggaattct
     4081 ctgcccagaa gtaataattt atatccccat aagaaatggg aacgcttatt ccataaatgc
     4141 catcattggc tgcgccatcg gaatgagctc catcatcaaa catttggtaa taagtgaatt
     4201 tgcttgctat gttttgcctg atgccaagct gagcataagt tgcattggca aaagtagctg
     4261 taaattgaag agtggaactt ggttcgggat tttcaggact gtagctctga gcagtcattg
     4321 tcggaactgt tcccgcaaaa ttagtattac ttaaaagata agtagcgcgg gcattcatca
     4381 attgtgtaat accaggaacg gtttgtcccc ctccgggacc tcctgaacca cctccaacct
     4441 gattatttag gtttgcttga aaattggcat aggtataaaa gtaattggga tcgttttgta
     4501 cggaaggtcc gcagacgctt tgcaattcag ctgccctggt tgcataccag ccattggaaa
     4561 aattttcctc tatcatagtg cgcatatgag caatatacat ctttttgtaa cgaggattaa
     4621 ttaacacatt ctttagtaac aaaaaagtgt tgctggtgga attgcgcaaa gggtccaaat
     4681 tctgcatact ggaaacagaa ccaccttgaa atccgccaaa ggacatattc aaatcccata
     4741 aaagcgggtt aattctatta tcggcatccc cgaagagata aaaattatga aagacattga
     4801 taggtgaatc caaatttacc aatagattat cataggcaca catccatata ttctgatcca
     4861 cattcatcac ctggtctata ttggcatagt tgttttgaag agtattggta aagttgatta
     4921 aagtgctcca tccatagtca gattccaaac cataatattg catataggca gtagaatcag
     4981 ctcctaaata accccaaaca gtaacagggc tcatagtatt tgtttcacat ttaaaccgcg
     5041 gcatcccgga agtatgaaaa tgttccctca taaaactact atccacatct tccactgaag
     5101 tgtaaactcc gataagtaca ttattcaccc aaacattggc ataattagct ttacaggcag
     5161 gcatatactt acgggcaatt tcgtaggaaa gagtttcccg cacgaaggag ggatcacaaa
     5221 agccattggc aagcttaaga gttccatatt tcccatcaat gagttgatct tcaattatgt
     5281 gatctaattt aatattgaaa ggattcttgt ttctgttggc gctgtaggaa ctattgcctt
     5341 tatagcgaac gcctacacta tcataagtta ccccgttaat aattgctgtg ccaatcaatc
     5401 tatcttctcc cgctgcataa agctggtcga gaatttgatc ccaattggat tgtgtaaaaa
     5461 ataactgaat cgtattcacg gtactcatat cgtaaaaatc tgtttgggct cgcatactaa
     5521 aagcaaagct aagtatcaaa cagagaacaa gatatgcttt tttcatattc acctcgttaa
     5581 aaaaatacag ggactgcttt ctacaatccc tgtactcaat actctcagga tttgttaatc
     5641 ctgcattttt ttattcgctc aagtagctaa gcacctggtt tgccttattg ataaagcttt
     5701 taagttcttt accactgaaa ggtttttgct ccaagagtga tagatcgtga atataagagc
     5761 aaagtgtttt cactacatca tctttaccct gctcagcgta ggcgagaatc tttttgatta
     5821 tcggattatc gcgatttaca accagagtgt gatatttaag catatcggaa gaagcatttt
     5881 ggtgagacag catatccata tcatgccatc tgcgcatata ttcactgaag acaatcattg
     5941 aaggtatctc cgggcttttc aagcttttca cttctacctt gatttccgta aaagcattat
     6001 cttccagagc tttcattgct tcagggccaa tctctttgcg tacattatct ggcagttcat
     6061 agggtttgat gatggtctgt tcatcatttt gaataagata aggacttaag atagtggcag
     6121 cattagggta tttttttaag aaatctgcat agctttcttt gctgaaactt gcttccactt
     6181 tttggcctaa tgccgtgtaa aagagttctt taagcttatc ttcagaatcg ctggcaaccc
     6241 cttctttgtt taccaagagg tcgttcactt cgctatctat tctcataaat tcgatattac
     6301 ccagtttatt ttccagctgt tgatagagat gagtatctaa aggactgttt tggaaaacaa
     6361 cttcaatacc ctgttctttc atcagattta aataactaac ttgagtatct tcactcgctg
     6421 cataccagat ttttgtttta tcaccttcgc ttttattgcg agctttatat tcttcaattg
     6481 taacataatc gccgctggca gttttaaata taatgatatc cttcattgct tcaaagaaat
     6541 catcttcttt cagcaagcca aatttgatga aagcattaat atcttcccag taactctcat
     6601 atttcttacg gtcttctttg aaagtattat taaaatgatc agcaaccttt tttacgatat
     6661 attggctgat tttcttcacc tgggcgtcat tctgtaaaaa actgcgggat acatttaagg
     6721 gaatatcggg gatatcaatt ccacctttca acaatagcaa aaattccgga ataaggtctt
     6781 ccagattgtc tgctacgaaa acattattac aatagagttt cacttctcct ttgaagaaat
     6841 ccggctcatt gcgcagtttc ggaaaatata gaatcccttt cagattgaag ggaaaatcca
     6901 cattcagatg aatccaaaac acaggatcca tataatcgtg aaatacattc ttgtaaaatt
     6961 ctatatattc ttcatccttc acatcgctag gtttacgatt ccacaatgct tctttttggt
     7021 ttacctgctt gccttcaaac ataattggat agggcataaa attgcaatag cgatccaaaa
     7081 tttcccttat cttaccttca ttggcatatt ctgcactctc ttcattcaga taaatagtta
     7141 tcgttgttcc aatctctttc cgcttacctt tgctcatcag atattccgtg ctgccatcac
     7201 attcccaaaa tgcagcttca ctaccttcag tgcaagatag tgaatctata gttactttat
     7261 cggctaccat aaatgcagaa taaaaaccta agccaaaatg accaatcata ttgttctgga
     7321 catccttata cttattaata aaatcctctg cacccgaaaa agctatctga ttgatatact
     7381 tctttatctc ttctgccgtc ataccaattc cactgtctat tacctgaatg gtctttttac
     7441 ctttatccaa cttcacttcc actttcatct cttcttcctt gaagttcgga tcagcatatt
     7501 tccgtttgga tattgcatcc acagaattag ataccagctc tcttaaaaat atatcatgct
     7561 gcgaatatag ccatttcttg ataatcggaa agatgttttc tgtatggatg cttaagcttc
     7621 ctttttcttt cgttttcttg tctgccattc tattatctcc tttattcatt aaattcattc
     7681 tgatgatata ataatcgaga cagggatttt gtcaagtgtt tttagcagtc ctctttctag
     7741 actgctaaaa acccaaatct gcttcagaca ctaattggat cactattcta tgttaaattc
     7801 tgcttgacta atttgggtta agatggaaca atgtaattta gcgaattttg atattttata
     7861 aagcatagga gtatagatga aaagaaatct aatagcactg agcttcctga tactgtttat
     7921 acccttggta gcagtaattc aggaaaccgc ttccttaaag cattttcttt acgggcacga
     7981 accaaattgt gcttatgata actggatttc ccatttagcc gaaggggtag taatccaagg
     8041 gtataacaca tatgccccct atgatagaca gacaaacggt tttggcaatt ttgttattgc
     8101 tactactttt cagctaagca gttggggtaa tattgttgat ttatttttag aaggtatgtt
     8161 agatgaagct cagtccgcaa ttgatgcttc cggttttccc tatcaagttg ttatttttaa
     8221 tgatacggac actaatatca cttaccggat gttgcgagaa attcctgata gcagttatgt
     8281 agatcaaaat ggaacagaag atgattatga tgatgaaata ggtgcttttg catatggttg
     8341 gggtttgtat atttataatc ctgaagggac aagacccatc attgttacag cacctcatcc
     8401 ttgtgacgat tttcctactc cagcttttgc tttggaatcg tttaaaatta tggatgctca
     8461 atttttgcta ataaacggtg caggcagaga agttaaatgg actaatagtg gcagttatac
     8521 aaatactaaa tcactttctg atcctactcg agtagccaat catcccttca atacctgtta
     8581 taaaaagttt gtcgataagc taaggattgt ttttaactgc agagaatttt cccctcagat
     8641 tcatagttat gattggaatt atcatgctgg ttatcccaat gtgcaaattt ctgcaggcaa
     8701 cagtcgtctc tgtcctaatc ttcctattag agatctttcc agtttgaagc ttgatatgat
     8761 aaaccaaggc catcatataa tggtgccagc taacactgtt ggttttcata gtgaagttga
     8821 actaaatgat ttttattcag tatattatga cacctatcct tttattttcg atgatggtga
     8881 acatagctat gaagtaaata attatattga tttaccggct tattcgcaaa actatcaaat
     8941 gctatatagt ttggaaaact ggactgatta tgatgtatat gatcctttcc tccatgttga
     9001 aatggacgaa ttacccaatt cgtatgaatt aactgaaaac acttatcgat ggttttatgg
     9061 ctggaatgaa gcacagcaac gctggaattt tgatcatctt tttgataatt ttttgcaata
     9121 ttatctacgt tgggtttacg atctaaatag tgtattgaat actatgttcg ctatgaatga
     9181 tgggcttaat cctcctactc caacaggttt tgcaatacat aatcaatctt tatattctgt
     9241 tactttacaa tggaataaag ttgattgtta cgattttgac acctttgaaa tactatattc
     9301 tacagatcct attggaacca ataattatca ggtttataac cgcagtaata atgctatgct
     9361 tgcgtcccca tattgtgaga gtattacagt tccaggcctt agcagttctt ccagttattt
     9421 ctttaaaatc cgaacaaagg ataaaaatgg aaactattct gatctttcta atcagataac
     9481 cactatacct gcacctgcaa atgtttatac atttactgct catggtatgg ataatgcagt
     9541 tcgtctttat tggggtgttt caggtcagaa taataatcag ggtttcagtg tgtaccgtaa
     9601 aaactctaca cagacagatt attccctgat agattcatat ctaactaatt cttctcttaa
     9661 caatccaact gcttccagtt ttacctattg ggactatgat gtagctaacg gacaggattg
     9721 ggaatatatt atcagctgca ctaataccag taatcaggaa tttttctata actatcctgc
     9781 aacagcagct gttagagcta ttcatagctt gtatataaga aataatactg caactttggc
     9841 agatacaatt tcctttgccc aaaattcttt tgcttcagat gctcaggatg ccttctatga
     9901 tgtaacaaaa ggtagtcctt ctggttcaaa ttatgtatgg gcagcctttt gggaacagta
     9961 ttgggggaat aatggaacta ctttatctcg agaagtaaaa ggtggatata ataccgcgct
    10021 cgatattaaa acatgggcaa ttagaatccg ttctactgaa ctaaatacac cactatatat
    10081 tagtgcttcc gataatttta atagggcaga aaagctctat ctttatgatt cttacacggg
    10141 aacctggcac aatttattca gtggacccta tcaatttact gtgcctaatt ccaatgtgag
    10201 aacgatgacc ctatattggg gtaatttaca acccaaggtc gttcacagca cccaaagtaa
    10261 ccaattatac caaggtggaa ataatatacc tttgcagtgg agctgtcaaa acaacttcct
    10321 ggttgatcac ttggatctat atataaagaa tgaaacggac agcttgtttt tagtagctaa
    10381 tgttcctgga aatcaaacta actttactta taatgttcct cagaacctgg atatgcaatc
    10441 tgctcacttt tatatagatt gtcatgcagt agatggattg atgcaaactt accgctcatc
    10501 ctacactttt gctattgttc cccgaatggt tttacatagt aatgaagcag gttggcagac
    10561 ccgttctcat atttggccag atttaactcc gacggtagaa aatatttttg ggactggagc
    10621 taccgctttt atcgccgggg ataataacac ctgggttgaa tgtgatgatt ttctttttgg
    10681 aattgcctat tgggttaatg cccccgatat gaatttcttc agttctactg cagacatctg
    10741 cgcttctgga atagattctt ttcctttgga acctggctgg aattttgtag ccaatcctca
    10801 ttattgtcgt tatccgattc atagcttacg ttttctggtt ggcaataatc tcttcctata
    10861 cagtgaaatg attgcccagc atcttatctc tcgagcagtt tatgtatatc ggaatggtag
    10921 gtttcaggca gtggatgtta ttgaaccttt tgaatccttt tttattaaat attactgcga
    10981 tcaatcactg aacacctatc ttagttttta cccctatttt gaagcaccag aaattactcc
    11041 tcctgataat ttctggcagt tccaagtggc ggtcacttgc cctgccagtg acactgatga
    11101 atttattttg ggtactaatc ctatcgctac agatgggtat gatttttatg ttgatttacc
    11161 agctgctcct gttaaacctt tcccttctat cagtgcatac ttaactcgtg aagcacctga
    11221 ggatataagt tttacggata aaaaactaag ctgcgaattt cgtgaggttt tttctcccac
    11281 tgtagatcag gaaaaaattt ggcatttcaa atttacctgt agctcagtag agccaattga
    11341 atttgatttg agcgacatca atctaccaaa tgattatacc attagaatct ttctgggaga
    11401 aaatatctac acttacagac agctgactca ctttacttat gttcctgcag aaccaggaac
    11461 ttatgaaggt ttaattaagg taacaaacta ccctgtaagt gtaacagatc tgattcagaa
    11521 gcctgtttca gcacttgcag tatatcccaa tccttttaat ccgaccacca caattgcctt
    11581 tagtacttcc caaacccaag ttgtggaagt ttccttgtat aacctgaagg gtcaaaaagt
    11641 ccgcacttta cataaaggaa tattgaagag tggcgaacat aaattgattt gggacggaaa
    11701 agataacaca ggtagaactg ttagtagcgg tgtctatttt accagaattg aaacaggaaa
    11761 acaatccttt acccgtaagc tgttattaat gaaatgagct tgctaagcaa gaaagttacg
    11821 ataacaaaga gctaaggaca gaatttaacc aagtagacaa ctttaggtta aagaagctac
    11881 ttcaaagaaa acaatagaat gcaaattagg gcagggaaat ctaatccctg ccttattatt
    11941 tataagataa gattgtagaa atatagacaa taaatagtat gaagtaattc ttgacaaaac
    12001 aaatgagaat atctaagtgt ttccatagtt attaaggagc ctatcatggg aaatcaaacg
    12061 atgactttag ctgagcaaga gaatataaac cgcatagcta atcaaaagca ctttcttaaa
    12121 gaaatagatt tgataataga ctggactaaa atcaataaag tcctatccaa agtagaaatt
    12181 agacgcgaat ctgtcgctgg cagtgataca tattcagccg aagttatgtt tcggattttg
    12241 ctgattcagt cctggtatca actatctgat tatcagctgg aagagcaact gacttacaat
    12301 ttgatgtttc tgtactttag tcatctcagt ttagaaaatc ctattccaga tcattcaacc
    12361 atcagtcgtt ggcgcagtag gttttcggaa aagaacatct ttgatgagct cttagaagag
    12421 attaatcgac aattggttat gcataacata gaggttcacg aaggcgtaat agttgatgct
    12481 actttagtag aatcctaagc ttgtcctcgt cggaaagaaa taatagagac agaaccagta
    12541 ggcgatgaag agattcctga gcagcaattt tatcaggcga cagagttggt tgtagaggaa
    12601 tcaaaagata aggatgcaaa tggttgaaga aagggaagaa atgtgttttt atttttgttt
    12661 tgacttacaa ttttaccact accctctctg taaccacatc cctaagattg cacacatttt
    12721 caaccgaatt ataattggtc tcctaacttc aaaattgtga gttaaaatat gaatcctaca
    12781 cccttgcaac cgagctgtaa caattcttca aattccacaa tcgtgaatga agttgttttt
    12841 tcacatttgc agggatgggt tggaatggtt cagagatagg gacttcggga cggatttttg
    12901 ttttgactta caattttgcc actaccctct ctgtaaccac atccctaaga ttgcacacat
    12961 tttcaaccga attataattg gtctcctaac ttcaaaattg tgagttaaaa tatgaatcct
    13021 acacccttgc aaccgagctg taacaattct tcaaattcca caatcgtgaa tgaagttgtt
    13081 ttttcacatt tgcagggatg ggttggaatg gttcagagat agggacttcg ggacggattt
    13141 ttgttttgac ttacaatttt gccatatctt aactttttct tttgatgttt cttcccggtt
    13201 tcacttttcc acctttccac tattcacctt ttcactttga aagtgatctt ttctggtata
    13261 ggttaaaaaa cttaggggat agcccgtaga agaaataata tccttttttt ccttcaacaa
    13321 ccattcatct tcattataag gagggaaaaa agtatcccca tcaaacttag aatgaaccat
    13381 agtaataaac agtttatccg ccaaaggtag aaattgttta taaatttgag cgccccctat
    13441 tacaaatgct tcttgtccat caaccagctt cagggcttct tctatgctat gaacaacaat
    13501 ggctccctct aattggagag atacatcttt actgataaca atattttgcc tacccggtag
    13561 tgcttttcct atagatttcc aggttgtttt tcccataatt acaggataac ctgttgttat
    13621 tttcttgaat ctggtaagat cttcaggaat atcccagggc aatttcccat ttttaccaat
    13681 cacccgattt aaatcaaaac cgactataat agagatactc agaatttacc tactttaaaa
    13741 aaggtatcaa tttgaggaat acctgcattt tttgcgtctt ccagagttcc gttaaaaagc
    13801 attttcccgc tatccagaaa aatgatttta tcggctattc tatgtatgga agccaattcg
    13861 tgagtaacaa ccacaatggt cattttcagt tgttttttta gttccagaat caattcatct
    13921 aaggaagcag cagttaaagg atccaatcca gcagaaggtt catcacaaaa caggatttga
    13981 gggtccaaag caatcgctct tgccaaagca gctctttttt tcataccgcc ggaaagttct
    14041 gaggggaatt tataaagcgc ctcactgagg ttaacgagat taagcttcac tttaattatt
    14101 ctatctatta aattttggga aagatcagta tgcaattcca gagggatact aatattgtca
    14161 taaacggaga tagaattaag caaagccccg ctttgaaata gcattcctat cttttttaaa
    14221 atattggcca tttggatatc atccaaatct aaaacctctt ctccccaaat ctttacgcta
    14281 cccgaaaagg gctcaaccaa gcgcaaaata ttcttcaaaa gagtggtttt tccacatcca
    14341 ctccccccca aaataacagt tatctcacca ggaaagatat ccacgctaat attatccaga
    14401 acaatattat caccatattt agcaaccaag ttcttaacgc tgattatagg ttccataatt
    14461 ataaatacaa caaactgaaa atggcatccc aaataataac agcaaagata gaagctacaa
    14521 ccgaagaagt agttgcctta ccaactcctt cagctccacc tttaacttca aaaccgcaat
    14581 aggcaccaat tacaacaatc gtccaggcaa aaacaatact cttaccaaaa ctggttacta
    14641 tatccttcat ggatagaaca tcaatggaac gctgcacaaa agtataaaca ctgaggtcta
    14701 aatagtcaat agcaattata ccgcctccaa tttcagaaac cagtatagaa aacataacca
    14761 gaataggcat aacaactgtg atagcatgaa atttagggac aacaacatag cgcacaggag
    14821 aaagagccat aatcttcaaa gcgtcaattt cttccgtaac ttgcattgta gcaatttctg
    14881 aagctatagc tgaaccactt cttccagcta caataatagc cgttattaga ggagctattt
    14941 ctcgcaccat agaaaaagcc attaaatcgg caataaaaac atttgctcca aaattagcta
    15001 actgcaccga ggcctgcaat gctaatataa aacctactat aaaagatagc aaagctaaaa
    15061 tcggcaatgc ttgtgagccc aagagtgaac actgaataat aaaggaacct ttgcgctgtc
    15121 cctttctatt aaaaatacca attaagctcc aatagaatac ttctgaagcc aacatcatta
    15181 cctgatagaa attctgagct gccaaaacaa cagaatcacc tattttttca aagaaaccca
    15241 ttttgcgtgc tggggtagga gtttctattt ttaaagaggt aaaagtatct ataacctctt
    15301 gaatttgtgg ttgaacgccc tggaacatca ataatccttc agctgctaaa cgattatgca
    15361 attcatccag aaaagctaca cctgcactat ccaaatttga tattccacta aggtctatcg
    15421 tattaggact ttgattttta aggagtgtat taattctata taagacctca ggaaccgttt
    15481 ccttagtaag gtttccttcc agataaagtg tgttatttac aattctacag ggcatttatc
    15541 ctcttaataa aataacgaaa ggcgtaagaa agtagtataa tcgtaaacca tccagggttt
    15601 ttctgcttca tcataaaaag tattgaattt tacatctaac gagatatttc taaataattt
    15661 gaagctaatc cgattttcgt tttccaaacg ataagtatga tctggcacac ccatcctgaa
    15721 aagcacatcc aaagtagaat ttatgcttac aaacttaagt aaatttaagg cagaaaatat
    15781 gatggtagat tctatccctt tagaactgct gttcggttct tcaatatatc tatcataact
    15841 caatccggtt tgggtgtcaa ctatggtttt atcgaaaaca tagctgcttg tattatagtc
    15901 ctgcagccaa ccataaccaa ctcgtaagct tgtccaagct ctggggctga aatttatttg
    15961 ataagttaag ccggttcctt ctttcaaacg cagagggtag aaagcgattt ttgtttttaa
    16021 atccggttga tctaaaaggg ttgaacaaat atcaccttct gcgttttgaa gtattatatt
    16081 cttgttttcg gaaaaatagg ttgtctcatc aaaaaaatgc gtatttatgt tcattcttcc
    16141 atagaatgcg aaattccgca ggaatttctt cttttccata ggatataaca tcaacacgct
    16201 tttcagagaa taatcgtccg gactgaatat cagatcttga ttggatgttt tatttaagcc
    16261 cagatcataa atgcttcgag tagtaaaatg aaagggtttt ataagttcat gtgtatctat
    16321 actgttatca aattgaccgg caagtgaaac gctggaagtt ggctttttct tatctacatt
    16381 attattagaa gataaggata tattaccatg tatggctcct ctatgaattc tcttggaacc
    16441 caaaccgata tcttcatcag caaaaacacc tgcaccgatt aatacatttc cttcggctgc
    16501 aggatcaaca atcattgtta gcgtcttctt atcgccttct gctaaagcaa ctgtggcaaa
    16561 atcttttaaa tcactccaag agccaccacc caattttact aaatatgaac ctggaggtaa
    16621 aatccaaagc ttatctacgg ctccaacttc atcttcgccc atacttacca tccctccact
    16681 ataaatttta taaccttcac cttccgcatc ttttttccag agatcatatc ccattcttac
    16741 tctgcttttg gattcgtcca taatcacaat ttttaattcg ccccaattgg gttt
//
LOCUS       DAOSTH010000033        16773 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3533_length_16773_cov_13.108685, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000033 DAOSTH010000000
VERSION     DAOSTH010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 16773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16773
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3533_length_16773_cov_13.108685"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="PLB65_05235"
     CDS             complement(<1..867)
                     /locus_tag="PLB65_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOQ77966.1"
                     /translation="MFKNNTKILLLILLFSISLYLNADYYDSVINLTGDNLFYGLRSL
                     ISNNTNTSYNESKEVLFQTLDNFGGYVTCVYTGQEYNVGYNYNGSSNPNTEHTYAQSW
                     FNGTDTAKKKSDLHHLFITNSVVNSSRGNYPFDVVANHNTANVYYSYTPRQSYRGNNA
                     QNRMVFEPADEFKGNIARALLYFYTRYNGESLVQQNVNMLPTLLIWHSFDPPDNAELT
                     RNTGVYNYQNNRNPYVDHPEFVGKIWGGDAVSDDVYLSIPELYINAVYPNPFVSEIAI
                     TISTKKSTPLTTS"
     gene            complement(880..1488)
                     /locus_tag="PLB65_05240"
     CDS             complement(880..1488)
                     /locus_tag="PLB65_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HOQ77967.1"
                     /translation="MHKIIIDTFKSAQELISEVLNRETFLSQIEALAKQMAAVLSKKG
                     KLIACGNGGSMCDAIHFAEELSGRFQQDRQALPGIALSDPGYLSCVANDYGWENVFAR
                     GVEAYGESGDLLLGISTSGNSLNIINAIAAAEKKNCFTAALLGKEGGKLKGLCDYEII
                     VPSNNTARIQEIHTIVIHLLIELIENELFASTEENPNSKLYC"
     gene            complement(1501..2580)
                     /gene="add"
                     /locus_tag="PLB65_05245"
     CDS             complement(1501..2580)
                     /gene="add"
                     /locus_tag="PLB65_05245"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOQ77968.1"
                     /translation="MKIKMTKEFIKKLPKTDLHVHLDGSVRIDTIIDLAKKYKIKLPT
                     MDPIELRKLLVCGEQTTSLDDYLRAFPIVNLVLQNEEGLKRAAYELAEDAVAENVRYM
                     EVRYSPILHTDQGLKLTEISQAVIDGLKEGERDFGIKTGVIICGIRNMDPTTSLKLAE
                     LAVAFKNKGVIGFDLAGGEYKHPAKDHKEAFDLALHNNLNITIHAGEAYGPESIHQAL
                     HYCGTHRIGHGTRLVEDGDLLNYVNDHRIPLEICLTSNLHTRAVPDIRSHPLDFYIDY
                     GLRVTINTDNRTISNTTVTDEYMLAINELGLDYPTVKYLILNGFKSAFLPYKERVRLI
                     NQTLKEIDEIEEQELKTKVEVKESL"
     gene            complement(2723..3373)
                     /locus_tag="PLB65_05250"
     CDS             complement(2723..3373)
                     /locus_tag="PLB65_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77969.1"
                     /translation="MTIKVIVCGEGSTDVGKRDYETSQWIDGPAIAYIKNASTKKLQI
                     DGIEKNDLPKIQKVKSLKGHSVKARKLGIYAMLHSYKVAICYVDCDKNTFEDIYKDIT
                     EGFSGYQNKVIGIPMIPKAMIESWLVADENAFLRVFGKKPSKPYLPKEPESLWGKKED
                     PNSNYPKNVLKRVVSQFNDKPEDWRYALARNSSSKTLSIKCPVSYGKFLCDMDKIK"
     gene            complement(3363..4586)
                     /locus_tag="PLB65_05255"
     CDS             complement(3363..4586)
                     /locus_tag="PLB65_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ77970.1"
                     /translation="MLKQVTIKGFKGITDLTLNLDKINVLIGINSSGKTTILQALDLL
                     ANCVSRDVSEYLKDKNWKVSDIKSQISKSSLLSYNALFEFEENGNPFLLIWQIEFKLI
                     SATSVNLTKESILKVNGKWNKAYCNIDKQQKLFENAIPLYTYRDGIVIYEAFHDQKAK
                     NQESEFYLSLGSSGLKIIDFAGNKDICLLKEFLKHTTNFETLAAENMRRSSRGTTEDI
                     GIGGKQLAAFIKQMNPQQKEDYTKIVHSILNSMDSIDAYTKGTPGWVELFINEKFGSN
                     DIKVRPYHISDGTLRILAFVALLNTQYDLGLLMLDEIENGLNPYITTKIVELLYEFTS
                     QTKHQLLLSTHSSLMLDDFKPEDIIYVYRDNDGRIKAKKVFNTKKIKTFLDYMSPGEI
                     WINLTEKELLEPNDN"
     gene            complement(4677..5606)
                     /gene="rnhC"
                     /locus_tag="PLB65_05260"
     CDS             complement(4677..5606)
                     /gene="rnhC"
                     /locus_tag="PLB65_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HOQ77971.1"
                     /translation="MHKEIEIYLSHLLPELQKAGISIDEQQEIDYGVQLRVSRHNYKS
                     ILNIYYSQKKGISTIPMGLNNPLKKLLSDLCSFQEELDIKMPPMHNWNAWIGSDECGK
                     GDYFGPLIVCAFAFDKKQEKEFKNIGVMDSKKLKTDAIYRIAKHLYKNYANCINCIVL
                     KPEKYNELIAKFKLQRKSLDDLLAWLHSTAIINVQKNHPESEGALVDQFSISQKVWRS
                     LKEKKFPLPCIERTGAEVDPAVAAASIIARYQFLESFAAMREFYQLNFPLGANGTVIK
                     TAREYINKYGEKRLGEVAKLHFKTTNQIENLSE"
     gene            complement(5606..6880)
                     /locus_tag="PLB65_05265"
     CDS             complement(5606..6880)
                     /locus_tag="PLB65_05265"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HOQ77972.1"
                     /translation="MLYKEFLDHIYKRYSGNVKLELNRMTGLLSDMEDPERYLNGFHI
                     GGTNGKGSVCATLEALCLASGLKTALNTSPHLINYSERFRINGSELPFTTILSTFRRY
                     ETLFKKWDASFFEITTALAFAMFSKQNVEVAIIEVGLGGRLDATNLFTPDISVITNIG
                     LDHIKTLGGTLEIIAAEKAGIIKKGIPLIIGDMEESPRSIVEGVAMAKQAPVFRFGRD
                     WNTETLSDSLDGISFNYSFGKYEFENLQANLIGEHQVVNVGTALTAFLLYAEKRDIPI
                     SEEIIRSALKQINWKGRLQVLSQKPLVITDGAHNVHGAKSLLRTLDRLFPNRKLKFLV
                     SILRDKDYPDMISLFCSKAEFIYITKNQSERAASIEEQVEEVKKHNVPYKTVPTVAEA
                     YALAVKECKPDDILITCGSLYTVGEILAISMN"
     gene            complement(6995..7639)
                     /locus_tag="PLB65_05270"
     CDS             complement(6995..7639)
                     /locus_tag="PLB65_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77973.1"
                     /translation="MLRNQNGISFYTVLSIILFIALVFILALPNFFNLDKEKNQEDCI
                     NNMKQIWVATTDYMRDTNTDFNGDLSLLTKTPKAQDPKHTYLSSNLYCPETSRQKNDY
                     TVYGKYVAEQIGTEIKHNYGVIVYCPNLVKFPKHIIPKAFYENMDPTQLQNYIIDDID
                     YIDTETGSNGAKKVELIEKYIQIWKTDPDAFVKRKANTTALRAILFPDKFSVSQ"
     gene            complement(7695..8216)
                     /locus_tag="PLB65_05275"
     CDS             complement(7695..8216)
                     /locus_tag="PLB65_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOQ77974.1"
                     /translation="MKHKKRKKDNTRRIISIFLVLFLLLILVYNPISLRLTTIGVAIY
                     YGLNPVIFYRLIKTESSFRSFAISDAQAIGLGQIKESTAFYIHKEHKRGYLFLPLYNL
                     KLAAKYLNYLKKRYNDNWSLTLAAYNWGESNVNRRISSHQIEKDKDYRHLFSDIPETY
                     NYINKILYPKNKT"
     gene            complement(8310..8795)
                     /locus_tag="PLB65_05280"
     CDS             complement(8310..8795)
                     /locus_tag="PLB65_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOQ77975.1"
                     /translation="MAVYSVNEVIEMAVQIERNGYAFYNEAIKRSDLDEASKELLTYL
                     RDEELTHENTFLALRDEIDLEVLKLSIDWELIAEYLKAIVDSRIFNSESSALQLVANS
                     KDAREIINNAITFEKDTLLYFHTLSDNISDPKTHNALRKIINEEIRHILKLNEFKKKM
                     G"
     gene            complement(8985..9995)
                     /locus_tag="PLB65_05285"
     CDS             complement(8985..9995)
                     /locus_tag="PLB65_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/SPASM domain-containing protein"
                     /protein_id="HOQ77976.1"
                     /translation="MNKDNYLLAKAFLPKQRLWQACGIGCDYLLSLAIHKPVVNFYPP
                     ALMLEPTNICNLRCPLCLTGNGTLNRPKGMMSLALFKKICKDVQDKIGMLILWNQGEP
                     FINPDFYEMLKIASKHQFYTMTSTNASLELDIERIVKSGLNKIIISMDGITEETYNNY
                     RVNGDFELVLKNMQALVRCKEKLHSKTPYLVWQFIIMKHNENELEKVKHLAKELKVDK
                     LEFKTAQIYNGEDQAFLPNNSKYSRYQKNGDNFILKTKLKNRCRRLWTQPVINWNGEM
                     NICCYDKNGTIQIGNMEKNSFSELWFGKAMQNMRKAILKDRSAFEICRNCGEGIVQKI
                     KD"
     gene            10667..11779
                     /locus_tag="PLB65_05290"
     CDS             10667..11779
                     /locus_tag="PLB65_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77977.1"
                     /translation="MKVKVLFMLTLLIASLCAAIDFTYVDMENYGSGVSRIVVGFYGT
                     GNYEVIPNSDYVEVKIDNNNPSKVNISYEGGNIVRSIVQRNNSIRISVSPTFRLERMV
                     LEDPFRLVLDIIVAKPNRQQKLDIAEFYTSCGKLNSADKIYVELNRDYPNDGEVLYKW
                     ALLLEQRGSNRLKAIVAKIPKNSAYYSAGQDILNRVYGRKAAARTSSEKAAPQEKPKS
                     VEEPKPIEPLKANKEIPPAVETEQTVQSESLVATPPLPQDIQEAPLPSGSEESKLSFF
                     EKNPLLIVFFVLLLLLIIIIFIFIHSGKKKPKPVVFAPPENRKEGEALDTETLCRMVS
                     RLLINGWTNKQIAKELKLPEEEVERLVKLSHQGNYI"
     gene            11783..13309
                     /locus_tag="PLB65_05295"
     CDS             11783..13309
                     /locus_tag="PLB65_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77978.1"
                     /translation="MPAKICVIVLLLWCSLLPAMRFIPNSAFNPEVQNSLKPSLLSYP
                     QSAKDDSISTYGNGLHIPLVPIPRFTDAIMDFFRLGKANHSLMSYQSNDRKFKADMNV
                     TAGLEPVKKDDICSFLYGGIYLESQYGEHFELNTNWWNGIFLGDQKTAETSWLVDGYY
                     NYSNNKLNIDNLSGDISYNANNLTLALGRGKFPVGNAISGSIILNDEVNDYGYILAEA
                     RSGLFSISFMHASLMADSTYNIYNNPYYNSKNYPDKYLALHQFGFQPYSNLNFFLGEE
                     IIYGNRSLDINYFLPVAFWRAIEHNLWDRDNVMIYAGGSYRPIQSLFFYTQVLIDEFS
                     FSELGSDWWGNKYALQGGIKWKTPFALPESPFADITTELTAIRPFTYTHYLNHTMFST
                     DGKPLGYPKGSNIADFSCLLRLPYRNILLWQGSVSYSKQGSFGSDWSEDYHNAFPDSE
                     AMDNGTASWFQGTKTNTITLKSNVLIDVFAHHRLLFGWEGCHNDDWQNTLYSAWQLIF
                     "
     gene            13320..13733
                     /locus_tag="PLB65_05300"
     CDS             13320..13733
                     /locus_tag="PLB65_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77979.1"
                     /translation="MHKVDRLEFPKRYKLKQVVLVVVIILVIWGIFIQRSGLKKTASE
                     IQISDISISRYGTQFIELEYTLANTGKKDQEIALMAKVWDANGEELASALYSIKIKAN
                     SKSRRSKMLDKLNRALKEGERPYKAEISLYTRHIP"
     gene            14075..16384
                     /locus_tag="PLB65_05305"
     CDS             14075..16384
                     /locus_tag="PLB65_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HOQ77980.1"
                     /translation="MEANSKAPAPFVLPVIFWIIGIILAKIIQINILFLLILASGLLI
                     LGVFCKKIRLILLIILFLVLGMLRFSIVKKESSPLALILQENEHLQQEICFTVTNVFS
                     VEEMRYAVELDSLAGFPLQDNIILSCSEKILPGKSYCLLAEIYPLVRDPILDIYPNRY
                     SAIGYQLGKAKSIDAKDVRDYLGRLRYKLLSSLEIKLGDQADWAKALLFGESGLKQEY
                     INQLTEAGIIHLVVVSGMHIWLIYMIIVSFFRIFFRRELAEFLFLPSILLYASLNNWA
                     PSVTRSIIMISIVILARWLQSPVSGAQTMALSIFIITLLSPMQLFNIGLQLSYISILV
                     IFYGLPHFRLFAQNKILNNSVYKMLENIKEGILLSTLISIAITPFTLYYFGTASLNGI
                     IGNIIGIPLMSIMLPLSMLVLISPQGWYITKLFVLCYKALNSLWISWIQFCHTLPFSL
                     SANYLSLYQALALAIVILWCFLLIRSKYKTALKTLIPASLLVMGFMLIPNINKQDASI
                     YVFNCGMGDCTLIRFQDGKTMLIDTGGGKNFGTPEAFVNEVDYQQESWLSKNLLPWLG
                     KKGINRIDYLILTHLHNDHCGGLITLLNHKKIGHIFVSDESVKQDFWNYAQKQKYFGS
                     AKIHTITDTCSFFIDKARLKFLHPDKNYLPHNENNASLVCRLDTKKESILFTGDIESE
                     AEFYLVDNYAKELKANYLKIPHHGSKSSSSTEFLEAVSPQEVWITAPRKNIYRFPHPD
                     TMQRIQKQQAKILLTGNGTIRKIGIPAKE"
BASE COUNT         5201 a         3037 c         2978 g         5557 t
ORIGIN      
        1 ggaagtggtt agcggcgttg atttttttgt agaaatagta atagctatct cagagacgaa
       61 aggattggga tagacagcat tgatatatag ttctggaatt gataaataaa catcatcgga
      121 aacagcatct ccgccccaaa ttttgccaac aaattctgga tgatccacat aaggattgcg
      181 gttattttga taattataaa caccagtatt acgagttagt tctgcattat ccggaggatc
      241 aaaactatgc cagataagca aagtaggtag catattcaca ttttgctgaa ctaagctttc
      301 tccattataa cgcgtgtaaa aataaagcaa agcacgggca atattacctt taaattcgtc
      361 tgctggctca aagaccattc tattttgtgc attattacct cggtaacttt gccgaggggt
      421 gtaactataa taaacattgg cagtattatg attggcaact acatcaaaag gataatttcc
      481 ccgagaacta tttactaccg aattagtgat aaataaatga tggaggtcag atttcttttt
      541 agcagtatcg gttccattaa accagctttg cgcataggta tgttccgtgt tgggattgct
      601 gctgccatta taattgtagc caacattata ttcctgtcct gtataaacac aggttacata
      661 gcccccaaaa ttatccaagg tctgaaagag aacttctttg gattcattgt agctggtatt
      721 ggtgttattg gagattaaac ttcgcaaacc ataaaagaga ttatcaccgg tcaggttaat
      781 aacgctgtca taataatcgg cattaagata tagagaaata ctgaacagta gtataagtaa
      841 gaggatttta gtattattct taaacattgt aaccttattt tagcagtata atttggagtt
      901 aggattttct tccgtgctgg caaacagttc attttcaatc aattcaatta gcagatgaat
      961 aacgattgta tgaatttctt gaatgcgggc tgtgttgttg gaaggaacaa ttatttcata
     1021 atcacaaagc cctttcagct tgccaccttc ctttcccagc agagcagcag taaaacaatt
     1081 ctttttttct gccgcggcaa ttgcatttat aatattgagg gaatttccgc tggtagaaat
     1141 tcccaaaagt aaatccccgc tttcaccata agcttcaacg cctctggcaa aaacattttc
     1201 ccagccataa tcattagcca cacaacttaa atacccagga tcgcttaaag caatgccagg
     1261 aagggcttga cggtcttgct gaaaacggcc tgaaagctct tcggcaaaat gaatggcatc
     1321 acacattgaa ccaccatttc cacaggcaat cagttttcct ttcttgctta aaacggcagc
     1381 catttgtttt gccagggctt caatctggct taggaatgtt tccctgttta gcacttcgga
     1441 aataagttcc tgtgcacttt taaaggtatc gataattatc ttatgcaaat ttagcgctcc
     1501 ttataaactt tcttttactt caaccttggt tttcagttcc tgctcttcaa tttcatcaat
     1561 ttctttcaag gtttgattga tcagccgtac tctttcttta tagggtagaa aagcactctt
     1621 gaaaccgttt aaaataagat acttaaccgt tggataatcc aagccaagtt cattaattgc
     1681 caacatatat tcatcggtta cagtggtatt gcttatcgtt ctattatcgg tattgatagt
     1741 tacccgcaag ccataatcaa tataaaaatc aagaggatgg ctacggatat cgggaactgc
     1801 cctagtatgc agattgctgg ttaaacaaat ctccaaaggt atccggtggt cgttaacata
     1861 atttaagaga tctccatctt ccaccagacg cgttccatgc ccaattctat gagttccgca
     1921 ataatgcaat gcctgatgaa tactctccgg tccgtatgct tcacccgcat ggatagtgat
     1981 atttaagtta ttatgcaaag ccagatcaaa tgcttcttta tggtctttgg caggatgttt
     2041 atattcacct cctgccaggt caaaaccgat aacgccttta tttttaaagg caactgctaa
     2101 ctcagcaagt tttagagaag ttgtcggatc catatttctg atgccgcaaa ttatcacacc
     2161 ggtcttaatc ccgaaatccc tttctccttc ttttagaccg tctataactg cttgggaaat
     2221 ttccgttagt tttaatccct gatccgtatg taaaatagga gaataacgca cttccatata
     2281 gcgcacattt tcagctacgg catcttctgc caattcataa gctgctcgtt ttagtccttc
     2341 ttcattttgc aaaacgagat tcacgatagg aaaagcgcgt aagtaatcat ccaaacttgt
     2401 cgtctgctcc ccgcaaacca atagtttacg cagttcgatc ggatccatag tcggaagctt
     2461 tatcttatac ttttttgcca aatcaattat ggtatcaatc cggacactgc catctaaatg
     2521 gacatgtaaa tccgtcttgg gtagcttctt aatgaattct tttgtcatct ttattttcat
     2581 ttcttcttcc tctttcttgt ttaacagaca taatatttaa taaaagccaa atgtcaaata
     2641 gtttttatgt tatacaggct tagtaaatta ggcaagggga tttatagctt aaaattgcaa
     2701 gataattttc actctctaac ggctatttaa ttttatccat atcacaaaga aatttgccat
     2761 aactaacagg gcattttatc gataaggttt tagaacttga gttccgggca agagcgtagc
     2821 gccaatcttc cggtttgtca ttaaactggc taactactct tttcaagaca ttctttggat
     2881 aattactgtt agggtcttct tttttccccc aaagcgattc cggttcttta ggaagatagg
     2941 gtttagaagg ttttttccca aaaactctta aaaaggcatt ttcatctgcc accagccaag
     3001 attctatcat tgctttggga atcattggaa taccgattac tttattctga tagccggaaa
     3061 aaccttcagt gatatcttta taaatgtcct caaaggtgtt tttatcacaa tcaacatagc
     3121 atattgctac cttataagaa tgtaacatag cgtatattcc aagttttctt gcttttacgg
     3181 aatgaccttt caacgatttt actttctgaa tcttgggcaa atcatttttt tctatgccat
     3241 caatctgcag cttttttgta gaagcatttt ttatataggc aatggcggga ccatctatcc
     3301 actgagaagt ttcataatct cttttcccta catcagtaga accttcacca cagacgataa
     3361 ctttaattgt cattcggttc taataattcc ttttcagtaa gattaatcca gatttccccc
     3421 gggctcatat aatccagaaa ggtcttgatt ttttttgtgt taaaaacttt ctttgctttg
     3481 attctgccat cattgtcacg gtaaacatag ataatatctt ccggcttaaa atcatccagc
     3541 attaaagaac tatgggtgct aagcaataat tgatgtttgg tttgagaagt aaattcataa
     3601 agaagttcta ctatttttgt agtgatataa gggtttagtc cattctcaat ttcatccagc
     3661 atcagcaaac ccaaatcgta ttgtgtattc agaagagcaa caaaagccag gatacgcaaa
     3721 gtgccgtcac ttatatgata gggtcgcact ttaatatcat tggagccgaa tttttcgttt
     3781 ataaataact ctacccaacc tggagtccct tttgtataag catcaatgga atccatacta
     3841 tttaaaattg aatgcacaat tttggtgtaa tcttcttttt gctgaggatt catctgtttt
     3901 ataaaagcag ccagttgttt tcccccgata ccgatatcct ctgtagttcc tctacttgat
     3961 ctacgcatat tttctgctgc tagagtttca aaatttgttg tatgtttaag gaattctttc
     4021 agcaggcaaa tatctttatt accagcgaaa tcaataattt ttagccccga agaacccaag
     4081 gacaaataaa attctgattc ttgatttttt gctttctgat catgaaatgc ttcataaatc
     4141 acaatcccat ctctataggt gtataaagga atagcgtttt caaaaagctt ttgctgttta
     4201 tctatgttac aatatgcttt attccatttg ccatttacct ttaaaatgct ttccttagtt
     4261 agattaacac tggtagcaga aatgagctta aattctatct gccagataag aagaaagggg
     4321 ttcccgtttt cttcaaactc aaaaagtgca ttataggaaa gcaatgaact tttggatatt
     4381 tgggatttaa tatcggagac cttccaattt ttatccttta aatattccga tacatctcgg
     4441 gaaacgcaat ttgccaataa atccaatgct tgcagaatag tggtttttcc ggaagagttg
     4501 ataccaatta aaacatttat cttatcaagg tttaaggtga gatcggtaat acctttaaag
     4561 ccctttattg ttacttgttt gagcatctct acctccaaag gcagttaatt aattaatctt
     4621 taagcaatga tgttatattc tctgatagtg tcaaggtata attcgtaatc tatatatcat
     4681 tcagataagt tttctatttg gtttgtggtt ttaaaatgca gtttggcaac ttcgcccaag
     4741 cgtttttccc catatttgtt aatatattcc cgcgcggttt taataacggt tccatttgct
     4801 cccaagggga aatttagctg atagaattca cgcattgcag caaaactttc caaaaattga
     4861 tagcgagcaa taatagaagc agcagccacc gcaggatcaa cttccgcgcc ggttctttct
     4921 atacaaggaa gaggaaactt tttttctttt agggatctcc acactttttg agaaatgctg
     4981 aattgatcca ccagcgcacc ttcactttcc ggatggtttt tttgcacatt tataatagct
     5041 gtagaatgaa gccaggcaag caaatcatcc aacgattttc tttgtagttt gaatttagca
     5101 atcagttcat tatatttttc cggttttaaa actatgcaat tgatgcaatt ggcataattt
     5161 ttgtagaggt gtttggcgat tctatagata gcgtcggttt ttagcttttt gctatccatc
     5221 acgccgatat ttttaaattc cttttcctgt tttttgtcaa aggcaaaggc acacacaatc
     5281 agcggaccga aataatcgcc tttaccacat tcatcactac cgatccaggc attccaatta
     5341 tgcatcggag gcattttaat atccagttct tcctgaaaac tacacaagtc cgatagcaat
     5401 ttttttagcg gattattcaa ccccataggg atagtagaaa ttcccttttt ttgtgaatag
     5461 tatatgttta ggatgctttt ataattgtgg cgggatactc ttaactgcac tccataatca
     5521 atttcttgct gttcatctat ggaaatacca gctttttgca gttccggaag caggtgggaa
     5581 aggtatattt caatttcttt atgcatcaat tcatagaaat agccagaatt tctccaacag
     5641 tatataaaga accgcaagta attaaaatat catctggttt gcattctttt acagccagag
     5701 cataagcttc ggcaactgtt ggaactgttt tataggggac attgtgtttt tttacttctt
     5761 ccacctgttc ttcaatacta gctgcgcgtt ccgattggtt tttagtaata tagataaatt
     5821 cagctttgct acagaatagg gaaatcatat caggataatc tttatctcgc aaaatagaaa
     5881 caaggaattt taacttacgg tttgggaata acctatccaa agtccgcagc agagatttag
     5941 cgccatgtac attatgggct ccatcagtta ttactaacgg tttttgagaa agaacctgca
     6001 acctaccttt ccagtttatc tgttttaatg ccgagcgaat tatctcttca gaaataggta
     6061 tatccctttt ttcagcatat aagagaaaag cggttaaagc agttccaaca tttactactt
     6121 gatgttctcc gattaaattt gcctgtagat tctcaaattc gtatttgcca aaactatagt
     6181 taaagctaat tccgtccaaa gaatccgaca gtgtttcagt gttccaatcg cgtccaaaac
     6241 gaaagacagg tgcctgtttt gccattgcca caccttcaac tatagatcga ggagattctt
     6301 ccatatcacc tattatcaga ggtatccctt tttttattat tcctgctttt tcagccgcaa
     6361 taatttccag agttccgcca agagtcttta tatggtctaa accaatatta gtaattaccg
     6421 aaatatcggg agtaaagaga ttggtagcat ccaatctgcc tcctaagcca acttcaataa
     6481 tagctacctc tacattttgt ttactaaaca ttgcaaaagc aagggcagtt gttatttcga
     6541 agaacgaagc atcccatttt ttaaacaagg tctcataacg acggaaggtg cttaaaatag
     6601 ttgtaaaagg tagttcacta ccattaattc taaatctttc gctatagttg atcagatggg
     6661 gagaagtatt aagtgcagtt ttcaatccac ttgctaagca gagtgcttcc aaagttgcac
     6721 aaacacttcc tttcccatta gtgcctccga tatgaaaacc atttaaatag cgttcgggat
     6781 cttccatatc agaaagcaaa cctgtcattc tgttcaattc caattttaca ttaccagaat
     6841 agcgtttata gatatgatca agaaattctt tatagagcat ttggttcttc tcatttttta
     6901 tatttttatg ctgcttacta caaaaacagt tcaaacggca tcacgattaa tgatgctgtt
     6961 tgaactaacc gaaaaatagc ttttagataa tgcgttattg actaacgctg aatttatcag
     7021 gaaaaagaat agctcttaaa gcagttgtat tcgctttcct tttgacaaaa gcatcgggat
     7081 cagttttcca gatttgtata tatttttcta tcaattcaac tttttttgcc ccatttgagc
     7141 cggtttcagt atcaatataa tcgatatcat caatgatata attctgtagc tgagtgggat
     7201 ccatattttc atagaaagct ttgggaatta tgtgttttgg gaatttcaca aggttggggc
     7261 aatatacgat aacaccatag ttgtgtttta tttcggtacc aatttgttca gcgacatatt
     7321 ttccgtaaac agtataatca tttttttggc gactggtttc ggggcaatac aaattagaag
     7381 aaagataagt atgttttgga tcttgtgctt taggggtttt ggtaagtaaa gaaagatcac
     7441 cattaaaatc tgtatttgta tcacgcatat aatcagtagt tgctacccag atttgtttca
     7501 tattattgat acaatcttcc tgattttttt ctttatccag attaaagaaa tttggcaatg
     7561 ccaagataaa caccaaggca atgaatagga ttatacttaa gacagtataa aacgagattc
     7621 cgttttgatt acgcagcata aatcactcct gaaatgaatt atagaaatat gatttttatc
     7681 aagggctatt ctcgtcaagt tttattttta ggatagagga ttttattaat gtaattatag
     7741 gtctcaggta tatctgaaaa gaggtgtcga tagtccttat ctttttctat ctgatgagaa
     7801 ctaatccgac gatttacatt gctttcaccc caattataag ccgccaaagt taagctccag
     7861 ttatcattat atctcttttt taaataatta agatacttag ctgccaactt cagattgtat
     7921 aacggtaaaa acagataacc tcttttatgt tccttatgaa tgtaaaaagc ggtggattct
     7981 ttaatttgac caagaccaat agcttgagca tcggagatgg caaaactacg aaacgaactt
     8041 tcagttttaa ttaatcggta aaaaattact ggattcagac cataataaat agctacgccg
     8101 atagtagtca acctcaaaga gattggatta taaaccagaa tcaataacag aaacaacacc
     8161 agaaagatac tgataatcct tctcgtattg tcttttttcc tttttttatg tttcacttgc
     8221 tatctatact gcttatctgg ctacatttct gtttcgccgc aatccattcc tgtattttta
     8281 atgttaatat aggttttgtt aataggtagt tatcccattt ttttcttaaa ttcgttcaat
     8341 ttcaaaatgt gtctgatttc ttcgttaatt atttttcgta aagcattatg ggttttggga
     8401 tcactgatat tatcggatag agtatgaaaa tagagaagtg tatccttttc gaaagtgatt
     8461 gcattattaa ttatctctct tgcatctttt gaatttgcaa caagttgcag agcagaggat
     8521 tcgctgttaa aaatacggct atcaacaata gcttttaagt attcagcaat taattcccaa
     8581 tcgatagata gtttcaatac ttctaaatcg atttcatctc gcaaagccaa aaaagtgttt
     8641 tcatgcgtta gttcttcatc ccgcaaatag gttaataact ctttggaggc ctcatcaagg
     8701 tcactgcgtt ttatggcttc attataaaat gcatagccat ttctttcaat ctgcaccgcc
     8761 atttcgatta cttcattaac agaatatact gccatttttt cctcctatgg aatacgattt
     8821 ttttataata tatctcgcta tgaaaaaaag tcaaggaaaa agtgtaaaag caccttggaa
     8881 ggataggata ttacagaaat agagatttaa gtacagagta taattttcta cctacgctaa
     8941 atgtagttcc aaaactgaaa ttatgtttca agaaagccaa taagctaatc tttaattttc
     9001 tgcactattc cttctccgca gtttctgcaa atttcaaaag cggaacgatc tttcaatatc
     9061 gcctttcgca tattttgcat tgcctttcca aaccataatt ccgaaaaact gttcttttcc
     9121 atattgccaa tttggatagt tccattttta tcgtagcaac aaatattcat ttcaccgttc
     9181 caatttataa ctggttgagt ccataaccgc ctacagcgat ttttcagctt agttttcagg
     9241 atgaaattat ctccgttttt ctgatagcga ctatatttcg agttattggg cagaaaggct
     9301 tggtcttcgc cattgtatat ttgggcagtt ttaaattcca atttatctac tttcagttct
     9361 tttgccagat gtttcacttt ctccagctca ttttcattgt gtttcataat tataaattgc
     9421 caaacaagat agggtgtttt actatgcaat ttttctttac agcgaaccaa tgcctgcata
     9481 tttttgagga caagttcaaa atctccattt acgcgatagt tattatatgt ttcttccgtt
     9541 attccatcca tagaaataat aattttattt agtccgcttt ttactatcct ttcaatatcc
     9601 agttccaaac tggcattagt agatgtcatc gtataaaatt gatgttttga ggcaatcttt
     9661 agcatttcat aaaaatcagg atttataaag ggctcgccct gattccacaa aatcagcatt
     9721 ccaattttat cctgaacatc tttacagatt tttttaaaaa gtgccaaaga catcattcct
     9781 ttgggacgat tcaatgtccc attcccagtt aaacacaaag gacagcgtaa attacaaata
     9841 ttcgttggct ccaacattaa agcaggcggg taaaaattaa ccacaggttt atgaatagca
     9901 agcgacaaaa gatagtcaca tcctattcca caggcctgcc ataaacgctg ttttggcaaa
     9961 aaagccttcg ctagtaaata attatcttta ttcatatttt tgcttttttc atatatggct
    10021 tttgcgtcaa gagaaaagag ggaactcaga atataaatgt ttttgtaata taacaattat
    10081 gcccaaaaac agatagaaaa gtgcttagat cacagagttt aaagcgaaga gcattgaatt
    10141 tatttactgt tttttcttac ttcttgctta ataagctgcg gtagcgatgc ggtgctgtac
    10201 ctaggtattt tccccatcag catcgccaca accccacgct agcttataag caagaaatag
    10261 cccagcttcg taccctcaag cattatttat gcccctatta acatttggca gaatagcatt
    10321 ttagccggaa aaacagattg atgcttaccc tccttttttc tttattttgt tatgtttgct
    10381 ttattagtgt taaaaatagc ttgccaattt taaagcggca aaaattatag taaaaatgga
    10441 atagttcaga caagatagag ctatataaaa gggaagatat tagctgataa ggtgttaaca
    10501 atatggaact tagatgaaaa gaggaagtgt attgttgcat aggttttata ttttatcttc
    10561 tatataggct tagaattggg attgatttat acaagaggaa taagagtgta agtgagatga
    10621 ttgttgatat caggtttttt tcaaatatag agaaggagac agctaaatga aagttaaagt
    10681 tttatttatg cttaccttat tgatagcgtc gctttgtgca gctattgatt ttacttatgt
    10741 agatatggaa aattatggtt cgggggtttc gagaatcgtt gtaggatttt atggcacagg
    10801 taattatgag gttataccca atagcgatta tgtggaagtg aagatagata acaataatcc
    10861 ctcaaaagta aatatcagct atgaaggtgg taatatcgtc cgaagcattg tgcaaagaaa
    10921 caatagtatt cgaatttctg tttctcctac atttcgattg gaaaggatgg ttttggaaga
    10981 tccttttcgt ttagttttgg atatcattgt tgctaagccc aatcgtcagc aaaagcttga
    11041 tatagcagaa ttttacactt cctgtggaaa gctgaatagt gctgataaaa tttatgtgga
    11101 gctaaaccgt gattatccca atgatggtga agttttgtat aaatgggcat tattattgga
    11161 acaaagaggc agcaatcgct tgaaagctat agtagcaaaa atacccaaaa actcagctta
    11221 ttattctgca ggacaggata ttttgaatag ggtgtatgga agaaaagcag ctgccaggac
    11281 ctcttcagaa aaagctgcac cccaagaaaa acctaaatca gttgaagaac ctaagccaat
    11341 tgagccccta aaagctaata aggaaatccc ccctgctgta gaaacagaac aaactgtgca
    11401 gtcagaatct ttagttgcca ctcctccctt gccacaagat atacaggaag ctcctcttcc
    11461 ttcaggatca gaagaaagca aattatcctt ttttgaaaag aatcccttgc taatagtgtt
    11521 ttttgtgtta cttctgctat taattatcat tatttttatt tttatccata gtggcaaaaa
    11581 gaaacccaaa cctgttgtgt ttgctcctcc cgaaaataga aaagaaggcg aagctctgga
    11641 tacagaaact ctatgtcgga tggtaagtcg tttattaatt aacggttgga caaataaaca
    11701 aattgccaaa gaactaaaac ttccggaaga agaagtagaa cgcttagtaa agcttagtca
    11761 tcaaggaaat tacatctaaa atatgcctgc caaaatttgt gttatagtgt tattactctg
    11821 gtgttcattg ttacctgcta tgcgttttat tcctaattct gcttttaatc ctgaagtaca
    11881 aaacagtttg aaaccgagtt tacttagcta tcctcaatcc gcaaaagatg atagtatctc
    11941 tacctatggt aatggattac atatacctct tgtccctatt cctcgtttta ctgatgccat
    12001 aatggatttt tttcgcttag gaaaagcaaa ccattctctg atgagttatc aaagcaacga
    12061 tcgtaaattt aaagcggata tgaatgttac tgcaggtttg gagccagtga aaaaagatga
    12121 tatctgcagc tttctttatg gtggtatata tttagaatcg caatatggag aacactttga
    12181 gctgaatact aactggtgga atggcatttt tttgggtgat caaaaaactg cagaaacttc
    12241 ctggctggtt gatggctact ataattatag caataacaaa ctgaatatag ataatcttag
    12301 cggagatatc agctacaatg ccaataattt aaccctggct ttagggcggg ggaaattccc
    12361 tgtgggcaat gcaattagcg gtagtataat tctaaatgac gaagtgaatg attacggcta
    12421 tatattagca gaagctagat ctggattgtt cagcatttct tttatgcatg catctttaat
    12481 ggcagatagc acttataaca tatataacaa tccttattac aacagcaaaa actatcccga
    12541 taagtattta gcgctgcatc aatttggctt tcagccctac tcaaatttga actttttctt
    12601 aggtgaagaa attatatacg gaaaccgctc gctggatatt aattattttt tgcctgttgc
    12661 cttttggcgc gcaattgaac ataatctttg ggatagagat aatgtaatga tttatgcagg
    12721 aggttcatat cgtcctattc agtcgctttt cttttatact caagttctta tagatgaatt
    12781 tagtttcagt gaactgggta gtgactggtg gggaaataaa tatgcgctgc aaggaggaat
    12841 aaaatggaaa actccctttg ctttaccaga aagtcctttt gcagatataa ctacagaact
    12901 aactgctatc cgtcccttca cttatactca ctatttaaat catacaatgt tctctactga
    12961 tggtaaacct cttggctatc cgaaaggttc aaacattgca gatttttcct gtttactacg
    13021 cttaccatat agaaatattc tgctttggca gggaagtgta tcttatagca aacagggaag
    13081 ttttggcagt gactggagtg aagattatca taatgctttt ccagattcag aagcaatgga
    13141 taatggaacg gcttcctggt ttcaaggaac aaaaactaat actatcacct taaaaagtaa
    13201 tgtgcttata gatgtttttg cacatcaccg tttgcttttt ggttgggaag gctgtcacaa
    13261 cgatgattgg caaaatactc tatattccgc ttggcaatta atcttttagg acaaatatta
    13321 tgcataaagt agaccgtctg gaatttccaa aacgctataa actgaaacaa gttgttttgg
    13381 ttgtagttat tatcttagtt atttggggca tttttatcca acgctcgggt ttaaaaaaaa
    13441 cggcttcaga aatacagata agtgatatca gcatcagccg ttatggcact caattcatcg
    13501 agctggaata cacccttgcc aataccggca aaaaagatca agaaattgct ttaatggcaa
    13561 aagtttggga tgccaatggt gaagaacttg ccagtgctct ttatagcatt aaaattaagg
    13621 caaatagtaa aagtagaaga tcaaaaatgt tggataaatt gaaccgagct ttaaaagaag
    13681 gggaaagacc ctataaagca gagatatcat tatataccag acatatacca taaaagagtg
    13741 cgggagttca aagttcaaag tgcgggagtt caaagtgcgg gagttcaaag ttcaaagtgc
    13801 gggagttcaa agttcaaagt gcgggagttc agagtgcggg agttcaaagt tcaaagtgcg
    13861 ggagttcaaa gttcaaagtg caggagttca aagttataga tcagtaaact aaatagcaat
    13921 aataaagaga gctgataatt ggatatgaaa tggaataaaa aacctgatta tctgtgccaa
    13981 taggttctat ccgtgaaatc tatattttaa aactaaatct gtgttatctg tgaaatctgt
    14041 gagagaagag aacgaatatt aaaatggaga aaagatggag gcaaatagca aagcccctgc
    14101 tccttttgtt ttgcctgtta ttttttggat aatcggcata atccttgcca aaattatcca
    14161 gattaatatc ctctttcttc tcattttagc cagcggacta ttaattttag gggttttttg
    14221 taagaaaata cgcttaatcc tgctgatcat tctattttta gttttaggaa tgctgcgttt
    14281 ttctattgta aaaaaagaaa gctctccgct cgccttgatt ctacaagaaa atgaacatct
    14341 tcaacaggaa atttgtttta ctgtaacaaa tgttttctct gtggaagaaa tgcgctatgc
    14401 tgttgagctt gattcccttg ccggtttccc tttgcaagat aatattattc tctcttgtag
    14461 tgaaaaaatt cttccaggca agtcttactg tttgctggca gaaatttatc ctcttgtgcg
    14521 tgatccgatt ttggatattt atcccaatcg ttattccgct attggttatc agcttggcaa
    14581 agctaaatct attgatgcta aagatgttag agattatctt ggtagattgc gatataaact
    14641 gctaagttct ttagagatta aattgggtga tcaagcagat tgggcaaaag cgttactctt
    14701 tggagaatcg ggtttgaaac aggaatatat aaaccagcta acggaagctg ggataattca
    14761 cttggtagta gttagcggaa tgcatatctg gttgatatat atgataatag tatccttttt
    14821 caggatattt tttaggcgtg agctggcaga atttcttttc ctgccgtcaa ttttgttata
    14881 tgcaagttta aacaattggg ctccttccgt aactcgttcc ataataatga tatctattgt
    14941 tattttggca cgttggttgc aaagccctgt ttccggagct caaacaatgg ctctatctat
    15001 atttatcatc actttgctca gccctatgca attattcaat attggacttc agctgtccta
    15061 tatatcgata ttagttattt tttacggttt accccatttt cgactgttcg cacagaataa
    15121 aatattgaac aattctgttt ataaaatgct ggagaatatc aaagaaggga tattactttc
    15181 aactctgata tcaatagcca tcactccctt cactctctat tattttggca cagcctctct
    15241 aaatggtata attggcaaca taataggcat tcctcttatg agtattatgc tacctctttc
    15301 tatgttagtg ttaatctctc cacaaggatg gtatataacc aagctatttg tcctttgtta
    15361 taaagcccta aattcacttt ggataagctg gatccaattt tgccatactc tacccttctc
    15421 tttatctgcc aattacttat cgctatatca ggccctggct ttggcaattg taatcctgtg
    15481 gtgttttttg cttattcgca gtaaatataa aacagctctt aaaacattga ttcctgcttc
    15541 tttactggtg atgggcttta tgctgattcc caatattaat aaacaagatg caagtattta
    15601 tgtttttaat tgcggaatgg gagattgcac gctaatccgt tttcaagatg gtaaaactat
    15661 gctgattgat acaggtggag gaaaaaactt tggaactcct gaagcatttg taaatgaggt
    15721 agattaccaa caggaaagct ggctaagcaa aaatctgcta ccttggctgg gaaaaaaagg
    15781 cattaataga attgactatc ttattctcac gcatttacat aacgatcact gtggggggtt
    15841 gataacattg ctgaatcata aaaaaattgg gcatattttt gttagcgatg aaagcgtgaa
    15901 acaagatttc tggaactatg cccaaaaaca aaaatacttt ggctccgcta aaattcatac
    15961 gattaccgat acctgctctt tctttataga caaggcaaga ttgaaatttc tgcatcccga
    16021 taaaaattat ctaccccaca atgaaaataa tgcttcgctg gtttgccgtc tggatacaaa
    16081 aaaggaaagc atccttttca ctggagatat tgaaagcgaa gcagaatttt atttggttga
    16141 taactatgct aaagaactga aagcaaacta tttaaaaatc cctcatcacg gttcaaaaag
    16201 ttcaagctcc acggaatttt tagaagctgt ttctcctcaa gaagtatgga taactgctcc
    16261 tcgcaaaaat atttatcgtt ttccgcatcc ggatactatg caacgcatac agaaacaaca
    16321 ggcgaagatt ctgttaaccg gtaatggcac cattagaaaa ataggcatcc ctgcaaaaga
    16381 atgatgtaac taagctataa cattttgtct tatagttaga aaacaagaga atatgcagct
    16441 ggttggaaaa attgactaaa aaatgaactt gacacaaatt gaaaagatga aaataagcag
    16501 ttaagaatag aatctaagta acgaaggata ttcgatgccc tcaaacaagc tgagaaacga
    16561 gttggaattt tataccaaaa tttgggactc aactaataag taaaatttgg caagagggta
    16621 ataagcaaag atattattag tagtagcata ataaaaaaag aagacatagg aaaatggcaa
    16681 gaatacaatc tacaggtaaa tgttgttttt gctcaagtac ttttaataag tcagcaatga
    16741 caaggcactt gaaatcctgt aaggaaagag aag
//
LOCUS       DAOSTH010000034        16182 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3706_length_16182_cov_9.966082, whole genome shotgun sequence.
ACCESSION   DAOSTH010000034 DAOSTH010000000
VERSION     DAOSTH010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 16182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16182
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3706_length_16182_cov_9.966082"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..2304)
                     /locus_tag="PLB65_05310"
     CDS             complement(133..2304)
                     /locus_tag="PLB65_05310"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOQ77981.1"
                     /translation="MVRFRYYFIAVAVLLIAILLPHLPIFSIVLKTLDLNIYDNILEV
                     YNAFSYNEYEGVYDNICIIDIDEKSIKDLGQFSSWPSLFFADLVNILAEDKPLAIAFD
                     IFFTESDSIKGYARKRLSSELTDITINPDKVMDKISTDDIFANAMKKAGMVYLAMFNS
                     DYPSYTQDLPDKLISWNVQPKHYLELKYPVPPIPQFTRTARGIGFANINPDITGKIHD
                     YPLFLKHKRDMYVNFSMQLYLDLFGIDKIRLDRSCHFFSGNKKVSELPLSPDGLFYFK
                     YYGPAKSFRYVSFSDVLFRKIPPGYFKDRIILIGTSAYGLKDNKITPLDRSFPGVELH
                     STFLRNLLEEDYIYWVNPWLLLIINFIALGIMAFIIPRSKPSISIGVFLLVTILFFPF
                     IYLLYVKASYIFSYSTILIPWVIGFLALFFTQAHEQGKEKRMVRNAFEHYVSPEVIQQ
                     IMKEPEKLRAGGEKKYISIMYVDVRNFTYLCEKLPPSEITSFMNSYFNLATQVIIENR
                     GTLDKYIGDAILALFGAPITYTDFERNAVKSALAIRKIASDIKKEHYSHPLLKDFHIG
                     IGIATGEVIIGNIGSDRIFNFTGIGDKMNFGSRLEGLNKYYQTTIIIDNFTFNAVKDV
                     FFCRKLDIVTVKGKSDVCDIYEVINTMDDMKNNPELSEIYHKYESALALMIAKNQSEA
                     KTLFEEVLLLLPNDEPTKIMLSRFDVINWETWDGTWQFDGK"
     gene            complement(2317..2988)
                     /locus_tag="PLB65_05315"
     CDS             complement(2317..2988)
                     /locus_tag="PLB65_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77982.1"
                     /translation="MKKYIITIILTAIVLFLFADSVGKIRFMVGEIQYKASPNLTYKK
                     ATLNMEVHSEGFIKTGPDSKAEIQWANGNVSIINANTEIRISKLQAEASSNPNYRNKL
                     WDRMNTLRVQSNKNQANNVAGIRREEVEVFPESQLYWYSEPKYKLEEAFTYFDAKDYQ
                     QAAELFEKIIEQAPLSKDAELSHTCLIIIYDQLGDSIKLKQHIKQLKEDFPDSSALDS
                     LPPEE"
     gene            complement(2991..7562)
                     /locus_tag="PLB65_05320"
     CDS             complement(2991..7562)
                     /locus_tag="PLB65_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HOQ77983.1"
                     /translation="MKKSLSMLAIIFIGWMSLWAIPQPLHKIYVSKDKKDSFAVDCFH
                     LEGHIAYCVDNSKGIIKAWDIHQQSFLQPVFIKLPLKAKVDDLSGDETALYILDSKMS
                     AIYVYDYFGTYVRTISTKGSADVQFIKAIRILVNYQGYIFVLDAGRNELLAFSNEGMF
                     LGKIPILAPISMCLGQDQIIRILVNRDKTQAIIPIDQNLNVGNSVTIAALDNKIDKIS
                     DIAMNQYNELYVIYSLSSKIGKVDASGKLINRSTWGSKDKSESMVSFQQPTIIKAIPN
                     YDKVLIGILDNKIRTLKLYQDSEFSSTQALEVPQLTLRPKLSESTEPQAIDYFACKDR
                     KYIIYNKTVQFNGANRATETISCIQNDTTIFNIYAVAEAKKGVQGYNALAIYNDKLFA
                     LDTKAAKVFVYNNLTGNYIESFAGKGSQDGHLDKPKSIAIDSDGMIYIADWGNSRIAI
                     FDENTAHIHNIELKAKGLKPQLLRISGQNLYFLANDSSIYQMFLNDDSTLKMITTAEK
                     ISTFDILYEDRLGYIDGITQQLNVIYDNNYEHRYFSKNAKGIFPGFGNIYLIRYNPYN
                     KTLYICDKMAKYTRLLTFYYSPKKPQTIYFTVNKAKQAELSWEAAEGISNWIVTEKKS
                     AETKTYQVNEPYFIVYEPQREICTYIVQSVSADNKVGPPSEEIEDAYSYARYLSLNKH
                     FAEAVQALQRAANTFSGVNFEDEMVQNFQQEARLYVSQNEFEKALNSIYSVEKIVGIR
                     IDTAIQKAEIYKIMNDYKQGIAHLEKFRNIEDKGIIRELISLYYLDGNYSKVKELCNV
                     YFSKFSRDTDILRYQIYADEKMKNYSAALNSMRELITQEDNLDNNIKMGGLLILNKDY
                     EAALNSLQRTLNRFNNQGADVIQKLMGDCYFNAGNYAYAVDKYLDAIRLNSEVAEYYY
                     CLGLAYTENRKAREATESFAKAYQSVPTEVKYGFAYAQALDKELRYPEALTVMDNIYQ
                     YVSSDSSSTAYHEFYHDLLIKEHRYDDAWREIQIALNYAPDNTTLQEKAVRTSDTRKY
                     YNENRDEIEIKKYEFYKLFPALIEYYQTHPIGTVTLFNTRNISIERITVTVTIPQITD
                     RPFQISIPTLMAGAELPIEITVPMNRNIFELCKNGVSTFNVNIQVDWIFDNKPKSVHK
                     YAVIQFQSINSMDWNERKQYACFVNPADENLRNFVTTQIIQLFSTQPVGTINKNIQRA
                     VQIWSFYSANGIKYISDISSANLTSSETDYVQFPFQTLHQKAGDCDDLLVLLASTLSV
                     IGIRCGFLDIPGHVMLVIDTGMNSEDILSNGFELSQFIFRNGNYWLPVESTLLGKETF
                     TTSWNEAVKHYNLLISKDIYPELMEFDIIHLLYPPAPYTEQISDYQFDKKEEVISSYR
                     QDLERITLMGTLTKEEEFIATLKKYPTNLNVINQYALWCLENKNPEKANELWSQILLK
                     DPHNLAALINLGNLQLSNDNFESARHNYLEALKQDKNTDPILRNLCILEYNSGNKLQA
                     KEYFNRMSDKTLLKKANPIIYSELLNIGE"
     gene            complement(8004..9629)
                     /gene="pruA"
                     /locus_tag="PLB65_05325"
     CDS             complement(8004..9629)
                     /gene="pruA"
                     /locus_tag="PLB65_05325"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOQ77984.1"
                     /translation="MFITVPVPKNEQLNSYAPGTCEREKLKKALHDLSNDFFEIPAII
                     NGKEVLTGNLGNCIEPHNHKHILAKYHKVGQAEIDMAIEAAMKARTDWEDMPFYHRSA
                     IFLKAAELLSTKYRYLLNAATMLGQSKNPFQAEIDSACELIDFWRFNTYYAQKIYEQQ
                     PLVSPKGEWNFSEYRALEGFIFAITPFNFTSIAGNLPTAPALMGNTVIWKPAGTAVYS
                     GHIIMRILKEAGLPDGVINFLPGSGTEIGDLIIDSPCLAGIHFTGSTKVFNNIWKRTA
                     NNIHLYKSYPRIVGETGGKDFIFAHSSADVKALAVACLKGAFEFQGQKCSAASRLYIP
                     DNLYDQWLKEMVEMLKTVKMGDAKDFSNFINAVIDKESFDRIKGYIERAKNAPDAEII
                     WGGECDDSVGYFIQPTIIKTTDPMFCTMQEEIFGPVLTVYVYPEKDYHKTLDLCDKTS
                     PYGLTGSIFAQDRKAILIANNVLKHSAGNFYINDKPTGAVVGQQPFGGGRSSGTNDKA
                     GSALNLQRWISARSIKENFVPDSDYRYPFMSES"
     regulatory      complement(9702..9818)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(9969..11066)
                     /locus_tag="PLB65_05330"
     CDS             complement(9969..11066)
                     /locus_tag="PLB65_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77985.1"
                     /translation="MKKPLCIVSALLLIGILGAINFDYDGELLTRAAAYNDAYKKDGG
                     HIDSRLRMGLNTQLTDGLDFRAAFEIGEITWGNNATGGALGTNGVNIETSELYLDYLM
                     NSINTNIKVGLQYWNDHRSLVLDDFFAGVMLYKDFNGMQAQLGILKKHEGLLNSHDDY
                     TVFLGSLEAKSPIVWGLTGFVGYNDIPDDGEITLLPYATIEAGPATIDVNPFLCYQFI
                     DGDDEIGIGASIKAGVDLTSIQLGADLLFASENALVTYSPYYQNGLFIYGINIIHDGL
                     DLYWDSNLYDHNSDALLSAVGSIRFPFKDKYALFAAAGIVNDLGMELNGGLECSVINN
                     LMNLKVYGAVGNNDDSDVTNYAVGTTLQLTF"
     gene            11463..11660
                     /locus_tag="PLB65_05335"
     CDS             11463..11660
                     /locus_tag="PLB65_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77986.1"
                     /translation="MFLSLVCFAYIIIFGDITEITTFYMSNNPYQIFGTVRVLDGVNL
                     TIESGFTVFFMQNSRLQIEGI"
     gene            12060..12329
                     /locus_tag="PLB65_05340"
     CDS             12060..12329
                     /locus_tag="PLB65_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77987.1"
                     /translation="MCNLTYTTIRNAGLDENYSYPDEFCSVYVYAGTVNLTDCTISLG
                     SFNLIKQEGNGVLNLTRCHITGGSNGIVHNNGNLTLTNCEISGCS"
     gene            complement(12367..13419)
                     /gene="arsB"
                     /locus_tag="PLB65_05345"
     CDS             complement(12367..13419)
                     /gene="arsB"
                     /locus_tag="PLB65_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HOQ77988.1"
                     /translation="MQQIANKKEGISFFEKYLTLWVIICMAAGVLIGKYIPGIPNFLR
                     KFEFAQVSIPIAVLIWLMIYPMMMKVDFTSIKNVGKNPKGLIVTWIVNWLIKPFTMYG
                     IAWFFFYIVFKSIIPANLAKDYLAGAILLGAAPCTAMVFVWSHLTNGKPAYTVVQVAT
                     NDLIILVAFTPIVAFLMGISGIKIPWDTLILSVILFVVIPLAGGITTRYSMIKHRGED
                     YFFKTFIPKFNNVTISGLLLTLVIIFAFQGEVIINNPLHIILIAIPLTLQTYFIFFIS
                     YIFTKQIKLPHNIAAPTALVGASNFFELAVAVAISLFGADSPVALATTVGVLVEVPVM
                     LNLVSIANKTRQWFIP"
     gene            complement(13526..14617)
                     /locus_tag="PLB65_05350"
     CDS             complement(13526..14617)
                     /locus_tag="PLB65_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HOQ77989.1"
                     /translation="MRYLPVDVLHNFMQEVFIKLGVPQTDAQICSDVLIASDLKGIES
                     HGVGRLKMYYDRIQAKIQNPITNIEVIRDKFATAVWDGNNGMGQVISQKAMQTAIDKA
                     EKYGLGAVAVRNSTHFGICGYYAEMAIKQNMIGLVFSNARPSICPTNSVSPILGTNPI
                     CFGAPTDLPYPFLYDAATSISQRGKVEQLAREEKETPLGWAIDLEGKSYTDTKGLLID
                     LLKQKASMLPLGGTEEITGSHKGYGLATAVEIICAALQNGNYLNSLMGEDEQGKPAPY
                     CLGHFFMAINIDFFTDLENFKKVTGSICRDLQNAQLFPGKTRIYVAGEKEYEIEQKVR
                     KQGVPINPNLEKNIRFIQDELSIDLLHLD"
     gene            complement(14631..15812)
                     /locus_tag="PLB65_05355"
     CDS             complement(14631..15812)
                     /locus_tag="PLB65_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HOQ77990.1"
                     /translation="MYKEPYSFNIGRFMDEKRFEKIKIFAEDIPTPCLIIDLDTIRNK
                     YLELQATMPNYKVFYAVKANPMDEVILLLAGLGSNFDLASRYELDQMLRLGIEPSRLS
                     FGNTIKKPEDIKYFHDKGVDFFATDSMIDLENLAKYAPGSKILFRILTEGTGADWPLS
                     RKFGSHPDTIYHLILKAASMNLQPWGISFHVGSQQRDIGQWDDAIARCKYLFDAVAEE
                     GIELQMINIGGGFPANYVDPTFSIEEYATEIQRFIQEDFGENLPDIILEPGRSLVADA
                     GVIVSEVVNISRKSKNNLYQWVYLDIGKFGGLIETIDESIKFPIFFPQIGMAEEIILA
                     GPTCDSMDILYEHFHYRMPEGTVPGDKVYIFTTGAYTQSYSSVNFNGFPPLKAVIYKE
                     L"
     gene            15959..>16182
                     /locus_tag="PLB65_05360"
     CDS             15959..>16182
                     /locus_tag="PLB65_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ77991.1"
                     /translation="MTVGTCKEINSAMTAFGYRVIGNYMFKKYIAKLAVGYPKMINYK
                     KEDLSMPKEFIYCRIAEVLSECIKFIVQYKR"
BASE COUNT         5234 a         2924 c         2652 g         5372 t
ORIGIN      
        1 tttattttag taataacaat ggttagcatt ctggtttgtt cagagaagac aataatgacg
       61 ggtatgatat caagtatcag cttaaagttt catcatcaat attcttaata gatcattcag
      121 aatagttatc gtttatttgc catcaaattg ccaggtgcca tcccaagttt cccaatttat
      181 aacatcaaag cgacttagca taattttggt tggttcatca ttaggtaaaa gcaataagac
      241 ctcttcaaac agggtttttg cttcactttg atttttcgca atcattaaag ctaaagcaga
      301 ttcatattta tggtatattt cagagagttc tggattattt ttcatatcgt ccatagtgtt
      361 tattacctca taaatatcgc atacatcgct ttttcctttt acggttacaa tatccagttt
      421 cctacagaaa aaaacatctt tcactgcatt aaaggtaaag ttatctataa taatggtggt
      481 ctgataatat ttgtttaatc cttctaagcg ggatccgaaa ttcatcttat caccaattcc
      541 tgtaaagttg aaaattctat ctgaacctat gttaccaata atcacttccc ctgtggcaat
      601 accaatacca atatggaaat ctttcagtag tggatggcta taatgttctt ttttaatatc
      661 agaggcaatt ttacgaatag ccaaagcact tttaacagcg tttcgttcaa aatctgtata
      721 agttatgggt gctccaaaca gagctaaaat agcatctccg atatatttat ctaaagttcc
      781 tctattttct ataatcactt gtgttgctag attaaaataa ctattcataa atgaggttat
      841 ttctgaggga ggtagttttt cgcacaaata agtgaaattt cgcacatcaa catacattat
      901 gctaatgtat ttcttttcac cacctgctct taatttttca ggttctttca taatctgctg
      961 aataacctca ggcgatacat aatgctcaaa ggcatttctg accattcttt tttctttacc
     1021 ttgttcatgt gcttgagtga aaaataaagc taaaaatcca atgacccaag gaatcagaat
     1081 tgtggaatat gaaaaaatgt agcttgcctt aacatataac agatagataa aagggaaaaa
     1141 aagtatggta actaataaaa acactcctat agaaattgag ggtttacttc gaggtataat
     1201 aaaagccatt atccctaacg ctataaaatt gataatgagt agcaaccagg gatttaccca
     1261 atagatataa tcttcttcca ataaattacg aagaaaagtg gaatgcaatt caactcctgg
     1321 aaaacttcta tctagaggag taattttatt gtccttcaat ccatacgctg aagttccaat
     1381 taatattatc cggtccttaa aatatcccgg aggtattttt cggaatagaa catcggaaaa
     1441 agatacataa cgaaaggatt ttgctggacc ataatatttg aaataaaaca aaccatcagg
     1501 acttagaggt aattcgctaa cttttttatt tccagaaaaa aaatggcaag aacgatccaa
     1561 tcttatttta tcgatgccaa ataaatccaa atagagttgc atactgaaat tcacatacat
     1621 atctctttta tgtttcaaaa aaagtggata atcatgaatt ttaccagtaa tatcaggatt
     1681 aatattggca aagccaattc ctcttgcagt tctggtaaat tgagggatag ggggaacagg
     1741 atattttagt tcgagatagt gttttggctg aacattccaa gagattaatt tatcaggaag
     1801 atcttgagta taagaaggat aatcactatt gaacattgct aaatatacca tccctgcttt
     1861 tttcattgca ttagcaaaaa tatcatcagt gcttatttta tccattacct tgtcaggatt
     1921 aattgttata tcggttaatt ctgaagacaa cctctttcga gcatatcctt taatactatc
     1981 gctttcggtg aaaaatatat caaaggcaat agcaagcggt ttatcttctg ccaaaatatt
     2041 tactaaatct gcaaaaaata aacttggcca ggaagagaac tgccccaaat ccttaatact
     2101 cttctcatca atatcaatta tgcaaatgtt atcgtaaaca ccctcatatt cattgtagct
     2161 gaaagcatta tatacttcca aaatattatc atatatgttt aaatccaatg ttttcagtac
     2221 tatgctgaaa ataggcaaat gaggtaataa aatagcaatc aataaaactg ctacagcaat
     2281 aaaataataa cgaaaacgga ccatatatcc tgttgtttat tcttctggag gtaaactatc
     2341 taaagctgag ctgtcaggaa aatcctcctt caattgttta atatgttgtt tcaatttgat
     2401 gctatctccc aactgatcat aaattataat tagacatgta tgagaaagtt ctgcatcttt
     2461 acttaatggt gcttgttcaa taatcttttc aaaaagttcc gcagcttgtt gataatcctt
     2521 ggcatcaaaa taggtaaagg cttcttccag cttatacttt ggttctgaat accagtataa
     2581 ttggctttca ggaaaaactt ccacttcttc tcttcgaatt ccagcaacat tattcgcctg
     2641 gtttttattg ctttggactc ttaaagtgtt cattctatcc catagtttat ttctataatt
     2701 agggttggat gatgcttcag cttgaagctt acttattcta atttctgtat tggcattaat
     2761 tatagacaca tttccatttg cccattgaat ttcagcttta gaatcaggac cagttttaat
     2821 aaacccttca gagtgtactt ccatatttaa agttgccttt ttataagtga gattgggaga
     2881 tgctttatat tgaatttctc ctaccataaa tcgaattttg cctacagaat cagcgaatag
     2941 aaataacact atagctgtta gaatgatggt aatgatgtat ttcttcatct ttactctcct
     3001 atgtttaaaa gctcagaata tataatagga ttagctttct ttaatagtgt tttatcactc
     3061 atccggttaa aatattcttt agcttgaagt ttattgccac tgttatattc taatatacag
     3121 agattacgga gaattggatc tgtattttta tcctgtttta atgcttccaa ataattgtgc
     3181 ctggctgatt caaaattgtc attacttagc tgtaaattac ccaaatttat taaagcagca
     3241 agattatgag gatcttttaa caaaatttga gaccatagct catttgcttt ttcaggattt
     3301 ttattttcca gacaccaaag ggcatactga ttgattacat ttagattggt aggatatttt
     3361 tttagggtag caatgaattc ttcttctttg gtaagggtcc ccatcagggt tatgcgttcc
     3421 aaatcttgcc tataggatga tattacttct tcttttttgt cgaattggta atctgaaatt
     3481 tgttctgtgt acggagcagg aggataaagt aaatgaataa tatcaaattc catcagctct
     3541 gggtatatat ctttactgat aagcaggttg taatgcttaa cagcttcatt ccagctggtt
     3601 gtaaaggtct ctttgcctaa taaagtactt tccacaggta accagtagtt tccgttcctg
     3661 aatatgaatt ggcttagttc aaaaccatta gatagtatgt cttccgaatt catccctgtg
     3721 tctataacca acataacatg tcctggaata tctaaaaagc cacatcgtat tccaataact
     3781 gataaagtag aggcaagaag aacaagcaaa tcatcacaat caccagcttt ctggtgtaaa
     3841 gtctgaaagg gaaactgcac ataatcagtt tcactggaag tgaggtttgc agaagatata
     3901 tcggaaatat actttattcc attagcgctg taaaaactcc atatttgcac tgctcgctga
     3961 atgtttttat taatagttcc tacaggttga gttgaaaaaa gctggataat ttgtgtggtt
     4021 acaaagttac gaagattttc atcagcagga tttacaaaac aagcatattg tttacgttcg
     4081 ttccagtcca tagaattaat gctctgaaat tgaataacag cgtatttatg cacgctttta
     4141 ggtttattat caaaaatcca atctacttgt atattcacat taaaagtact taccccattt
     4201 ttacataact caaatatatt acggttcata ggaacagtta tctctatagg taattctgca
     4261 ccagccatta aagttggaat actaatctga aacggtctgt ctgtaatttg aggtatagta
     4321 acggtaactg tgattcgttc aatagatata ttacgcgtat taaacagagt tacagtacca
     4381 attggatgtg tttgataata ttcaatgaga gcaggaaaga gcttgtaaaa ctcatacttt
     4441 tttatttcta tttcatctct gttttcgttg tagtactttc ttgtatcaga agtcctaact
     4501 gctttttctt gtagggttgt attatcaggg gcataattta aggcaatttg aatttctctc
     4561 caggcatcgt cataacggtg ctctttaatt aataaatcgt gataaaattc atggtatgca
     4621 gtagagctgc tatccgaaga tacatattga taaatattat ccataacggt taatgcttca
     4681 ggataacgaa gttctttatc cagtgcttgg gcataagcaa aaccgtattt aacttctgta
     4741 ggtacacttt ggtatgcctt agcaaaactt tctgtggctt ctctggcttt acgattctcg
     4801 gtataagcga gtcctaaaca ataataatac tctgccactt cagaattaag acgaatagca
     4861 tcaaggtatt tatctaccgc ataggcataa ttaccggcat taaaataaca atctcccatc
     4921 aatttttgaa ttacatcagc tccctgatta ttgaatcggt tcaaagtgcg ctgtagagaa
     4981 tttagcgctg cttcataatc tttatttaga ataagcaatc ctcccatttt tatattatta
     5041 tccagattat cctcttgtgt gattaactct cgcatagaat tgagcgcagc tgaataattt
     5101 ttcatttttt catctgcata tatttggtaa cgcaaaatat cagtatcacg gctaaatttg
     5161 gagaaataga cattgcacag ttcttttact ttgctataat ttccatccag ataatacaga
     5221 gaaattaact cccgtataat gcctttatct tcgatgtttc taaacttttc taaatgagcg
     5281 ataccttgct tgtaatcatt cattatttta tagatttcgg ccttttggat agcggtatca
     5341 attcgaatac ctacgatttt ttctacagaa tatatactgt ttaatgcttt ttcaaattca
     5401 ttctggctaa cataaagccg ggcttcctgc tgaaaattct gcaccatttc gtcctcaaaa
     5461 ttaactccag agaaagtatt tgctgcgcgt tgcaatgctt gaacagcttc tgcaaagtgt
     5521 ttatttaagg atagatagcg ggcatacgaa taggcatctt caatttcttc tgaaggtggt
     5581 cctactttat tatctgcaga aacggattgc acaatataag tacaaatttc tcgttggggt
     5641 tcataaacta taaaatatgg ctcgttaact tggtaagttt tagtttctgc actctttttt
     5701 tcggttacta tccagttaga tataccttct gcagcttccc aagagagttc tgcttgtttt
     5761 gctttgttaa ctgtaaaata gatagtttgt ggttttttgg ggctgtaata aaaggtcaat
     5821 aagcgtgtgt attttgccat tttatcacat atatataggg tcttattata tggattataa
     5881 cggattagat atatattccc aaaaccggga aagatgcctt tagcattttt ggaaaaataa
     5941 cgatgctcat agttattatc ataaataaca ttcaactgtt gcgtaattcc gtcaatatac
     6001 cccaagcggt cttcatacag gatgtcaaaa gtgctgatct tttcagcagt agtaatcatt
     6061 tttaaagtgc tatcatcatt tagaaacatc tggtaaatag aggaatcatt agccagaaag
     6121 tatagatttt gtccgctgat tcttaataat tggggtttca atcctttggc tttcagttct
     6181 atattgtgta tatgagctgt attttcatca aatatagcaa tgcgggaatt tccccagtcg
     6241 gctatataaa tcattccatc cgaatcgata gctatgcttt taggcttatc caaatgccca
     6301 tcctgcgaac ccttccctgc aaaagactca atataattac cggttaaatt attatacaca
     6361 aacaccttgg ctgcttttgt atcaagcgca aatagtttat cattgtatat agctaaggca
     6421 ttataacctt gaactccttt ttttgcttct gccacagcat atatattgaa gatagttgta
     6481 tcattctgaa tgcaagaaat tgtttcagtt gctctattag caccgttgaa ttgaacagtt
     6541 ttattgtaaa ttatgtactt tctgtctttg caggcaaaat agtcaattgc ttgaggttct
     6601 gtactttctg ataatttggg acgcaaggtt aactgaggta cttctagtgc ttgtgtggaa
     6661 gagaattcgc tatcttgata tagctttaag gttcttattt tattatccaa aattccgata
     6721 agaactttat catagttagg aatggcttta ataatagtcg gctgttgaaa agaaaccatc
     6781 gattcacttt tatcttttga accccaggta gacctgttta ttaatttgcc ggaagcatct
     6841 actttaccta ttttggaact taatgaatag attacatata attcattata ttgattcata
     6901 gcaatgtcac taattttatc gatcttatta tcaagagcgg ctattgttac actatttcca
     6961 acattcaaat tttggtctat cggaattatt gcttgagttt tatctctatt aactaatatt
     7021 cggattatct gatcctgccc caaacacata gatataggtg ctaaaattgg aattttacct
     7081 aaaaacatac cttcgttaga aaaagctaaa agctcattac ggccggcatc cagaacaaag
     7141 atataaccct gataatttac caggatgcga attgccttaa taaattgaac atcagcagat
     7201 ccctttgtag atatggttcg tacataagtg ccaaagtaat cataaacata aatagcactc
     7261 atcttagaat caagtatata tagggcagtt tcatctcctg aaagatcatc tactttcgct
     7321 tttagtggta acttaataaa aacaggctgt aagaatgact gctgatgaat atcccatgcc
     7381 ttgattatcc ctttggaatt atctacacaa taggcaatgt ggccttcaag atgaaaacaa
     7441 tctacagcaa aagaatcttt cttatccttg gatacataga tcttatgcaa gggttgaggt
     7501 atagcccaaa gagacatcca tccaataaaa ataatagcca acatagatag cgattttttc
     7561 ataatttctc ctacctgatt tctcctacac tatataataa gtaaaaaata gttaaaaaca
     7621 aacttccttt ttaccataaa gttttaagca aactgcacat tccttctggt tatataattc
     7681 gctcagtttc tccatttaaa aatctatcac ttagtttata ataataatat tattgtcaag
     7741 cagaatgtta ttcttgtata aaaaaatccc agtatttatg caggataatg tttatctgat
     7801 aattgcaaac aggatccgaa tttatgcaaa acttttctct agattgacta cagcattata
     7861 tgggatcaaa ttaaataaac gcagaattga agtatcaaaa tagattatgg gcataaaaaa
     7921 ggtggcaaat atcgttattg ccaccgtaca agttagagta ttccatcgat aaaataatct
     7981 ctatcgataa atgttagctt agcttaagat tcactcataa aggggtatct ataatcacta
     8041 tcaggaacaa agttttcttt gatagatctg gcagatatcc atctctgtaa atttaacgct
     8101 gagccagctt tatcattagt tccggaagat cttcctcctc caaaaggttg ttgaccaact
     8161 actgccccag taggtttatc atttatataa aagtttcctg cgctgtgttt taaaacattg
     8221 ttagctatca ggattgcttt tctgtcttga gcaaaaatgg agccagttag accataggga
     8281 gaggttttat cgcaaagatc aagagtttta tgataatctt tttcaggata cacataaacg
     8341 gttaaaaccg gaccgaagat ttcttcttgc atagtacaaa acattgggtc ggttgttttt
     8401 attattgttg gttgaataaa gtaccctacg gaatcatcac attcaccacc ccagataatt
     8461 tctgcgtctg gagcattttt agcacgctca atatatccct taattctatc gaaactttct
     8521 ttatcgatca cagcatttat gaagttcgaa aaatctttag catcacccat tttaactgtt
     8581 tttagcattt ctaccatttc ctttaaccat tggtcgtaaa gattatcagg aatgtataaa
     8641 cgagaagcag cactgcattt ctgaccttgg aattcaaagg caccttttaa acaggcaaca
     8701 gccaaagctt taacatcggc tgatgaatga gcaaagataa aatccttgcc acctgtttct
     8761 ccaacaattc tgggatagga tttatagagg tgaatattat tggcagtacg tttccaaata
     8821 ttattaaaga cctttgtact acctgtaaag tggatacctg ccaagcaagg ggaatcaata
     8881 attaaatcgc caatttcagt tccggagcca ggaagaaaat taattactcc atcaggtaaa
     8941 ccagcttctt tcaaaatcct cattattata tggccactat aaactgctgt ccctgcaggt
     9001 ttccagataa cggtatttcc cattaaagca ggtgcagttg gaagatttcc agctatggaa
     9061 gtaaaattga aaggagttat agcaaagatg aaaccttcca aagctctata ttcagaaaag
     9121 ttccattctc ctttgggcga aaccagtggt tgttgctcat aaatcttttg ggcataatag
     9181 gtattaaaac gccaaaaatc aataagttca caagctgaat caatctctgc ctgaaaagga
     9241 tttttacttt ggccaagcat tgttgctgca ttcaatagat agcgatattt tgtagaaaga
     9301 agttcggctg ctttcaaaaa aatagcagaa cggtgataga aaggcatatc ttcccaatcg
     9361 gttctcgctt tcatagctgc ttcaatagcc atatcaattt cagcttgacc cactttgtga
     9421 tatttagcca aaatatgctt atggttatgg ggttcaatgc aatttccaag atttccggtt
     9481 agtacttcct tcccgttaat tatggctgga atttcaaaaa aatcgttact taggtcgtgt
     9541 aatgctttct ttagtttttc tctctcacaa gtacctggag cataactatt tagttgctca
     9601 tttttgggga cgggaacggt tataaacatt tttatctcct tcagtttatt ttctttttac
     9661 aaaggataag gagaaaaggt aaggatattg tgcattacta tttgtagctc aattatacac
     9721 cttttcatac tggaaggcat atccgttttc agatataccc tttggccaac ttccatgctg
     9781 taaagtttag gattattagc tcggtgatat aaccagcttt taaatacagg ttattcagtc
     9841 aagcataata aaaatgttca cttcgttaac ttttatgaat agtctctgca atttgtagga
     9901 ttatccctaa actaaaaaaa cccggcttat aaaaaccggg tttggcaact ttgctaagta
     9961 gaaaactact aaaaagttaa ctgcagggtt gtgccaacag cataattggt tacatcactg
    10021 tcatcattgt ttcctacagc accatacact ttcagattca taaggttatt tataactgag
    10081 cattctaatc caccattaag ttccattccc agatcattta caatacctgc tgcggcaaat
    10141 agagcatact tatctttaaa aggaaaacgg atagagccaa cagcactaag caaggcatca
    10201 ctattatgat cgtacaaatt gctgtcccag tataaatcta atccgtcatg aataatatta
    10261 attccataaa taaagagtcc attttggtaa tatggactat aagtaactaa agcgttttca
    10321 gaagcaaaaa gaagatcagc tcccagttga atagaggtaa gatctacacc tgctttaata
    10381 gaagccccaa tcccgatttc atcatcacca tcaataaatt gatagcataa gaaaggatta
    10441 acatcaatag ttgcaggacc tgcttctatt gttgcataag gcaataaagt aatttcacca
    10501 tcgtctggaa tatcattata tccaacaaat ccagttagcc cccaaactat gggagatttt
    10561 gcttccaggg agccaagaaa cacagtataa tcatcatgag aattaagaag cccttcatgt
    10621 tttttcaaaa ttcctaattg agcttgcatc ccattaaaat ctttatatag cataacacca
    10681 gcgaaaaaat catctaaaac taagctccga tgatcattcc aatattgcag accgacttta
    10741 atattagtat taatggaatt cattagataa tcaagataca attcgcttgt ttcgatattg
    10801 acaccatttg tacctagagc tcctccggta gcattattac cccaagtaat ttcaccaatc
    10861 tcaaaagcag ctctaaaatc gagcccatca gtcaactgag tattaagccc catacgtaaa
    10921 cgactatcaa tatgacctcc gtccttttta tatgcatcat tataggcagc ggcacgagtt
    10981 agcaattctc catcataatc aaaattaatt gctcccaaaa tgccgataag caatagggca
    11041 gaaactatac ataatggctt tttcatcttt atctcctttc aatttttctg ttctctatat
    11101 aagataaact gatttgttca ggttgtcaaa taaaaaagtt gcaaaacatt atttgatttc
    11161 taatgctaaa ttaaataatc atcaattaac tgtttgcagg atataatgtt accatagcat
    11221 ttagccaata gatcagcatt aattagctta ttgctattaa gacaaactgg gtatcctgta
    11281 ttatgaaatc tactatttac gaagctgcca ctagttaaaa tagtagatga aagatgtcaa
    11341 gattatacca ttgaccgagt aagaataggc attatatctc ctaaggattt ttaatagaat
    11401 ttccaagtat ttcaattaca gaggtgtaaa atgaaaaatg aatgtatctt aacgctcgtt
    11461 ttatgttttt atctttagta tgttttgcat atattatcat ttttggggat ataaccgaaa
    11521 ttactacttt ctatatgtcc aataatccgt atcaaatatt cggaacggta agagttttgg
    11581 atggtgtaaa cctaactatt gagtccggtt ttacggtgtt ttttatgcaa aattcccgtt
    11641 tgcaaataga aggaatataa tattctatca atggcttaaa cataatggct gtaatgctta
    11701 ccagctaagt atttttgcta attggactca tcaattggat aatattactt taacggataa
    11761 tgcacttaat cttatccgtt tcatcaaagg tggtcgcttg gcaagtaaaa aatctgaaaa
    11821 attctggtta tccctatcta atatctcaaa ggtttactgt taataatgga gtaacattta
    11881 attatttatg ctggcaccac aatttacttt gccgattatg tagctaatag tgttaattca
    11941 tattttaccg tttatggggc attaaatact ttgggaacgg tatccgaact tgtctttttt
    12001 agaggaatgg atagtgacct aagttcctgg tgcggattaa aaataaacca gccagagctg
    12061 tgtgtaattt aacttataca actatccgca atgccggttt ggatgaaaat tacagttatc
    12121 ctgatgaatt ttgttctgtt tatgtatatg caggcaccgt gaatttaact gactgcacaa
    12181 tatccttggg aagttttaat ctgatcaaac aggaaggaaa tggcgttctt aatctgacgc
    12241 gctgtcatat tactggtggt tccaatggaa ttgtgcataa taatggaaac cttactctta
    12301 ctaattgtga gataagtggt tgcagttaga taacaaaaat atcgtaatat aatccaaact
    12361 caaattttaa ggaataaacc actgcctggt tttgttagct atgcttacca gattaagcat
    12421 aacgggaacc tcaactaaaa cacctacagt tgtagccaag gcaacaggtg aatctgctcc
    12481 aaaaagagaa atagctactg cgactgcgag ctcaaaaaaa ttggatgctc caactaaagc
    12541 tgttggtgca gcaatattat gaggtaattt tatttgtttg gtgaatatat agcttataaa
    12601 aaagataaaa taggtctgaa gcgtaagtgg aatagctatt aggatgatat ggagaggatt
    12661 attgataata acttctcctt gaaaagcaaa gataataaca agagtaagca acaaaccgct
    12721 tatagttaca ttattgaatt tggggataaa ggttttaaag aaataatcct cccctctatg
    12781 ttttatcatt gaatatcgcg tagttattcc tccagctaaa ggaataacaa caaataagat
    12841 aacggagaga attaatgtat cccaaggtat ttttattccg ctaatgccca tcaggaatgc
    12901 tacaatgggt gtgaaagcaa ccagaattat caaatcgttt gtagctacct gaactactgt
    12961 ataagcaggt ttaccgtttg ttaaatgact ccagacaaaa accattgctg tgcaaggtgc
    13021 agcacccaac aaaatagctc ccgcaagata atctttagct aaatttgcag gtataataga
    13081 tttaaataca atataaaaga aaaaccaggc aattccatac attgtgaaag gtttaattaa
    13141 ccaatttaca atccaggtta caattaatcc cttggggttt ttccctacat tcttaatgct
    13201 ggtaaaatct actttcatca tcatcggata gatcattaac catataagaa cagcaatggg
    13261 aattgagacc tgagcaaatt caaatttacg caggaaatta ggtattccag gtatatattt
    13321 acctataaga actccagcag ccatacatat aataacccac aatgtcagat atttctcgaa
    13381 aaaactgata ccttcctttt tgtttgctat ctgctgcatt ttatcctcct tctggttttt
    13441 atactctttt aacggtagat tataaattca gtgataaacc tgtaacaaac aacagcatta
    13501 tgtatatatc cctggtataa ttggattaat ccaaatgcaa taaatctatg cttagttcat
    13561 cctgaataaa tctaatattt ttttccagat tgggattgat aggaacgcct tgtttacgca
    13621 ctttttgttc tatttcgtat tctttttcgc cagctacata gattcttgtt ttaccaggaa
    13681 aaagttgggc attttgtaaa tctctgcaga tggagccggt aactttttta aagttctcca
    13741 aatccgtaaa aaaatcaata ttgattgcca taaagaaatg tcctaaacag taaggagcgg
    13801 gtttaccctg ttcgtcttcg cccattaagc tgttcaaata atttccattt tgtaaagcgg
    13861 cgcaaataat ctctacggct gttgcgagtc cgtaaccctt atggcttccc gtaatttctt
    13921 ccgttccacc caaaggtaac atcgaagctt tttgttttaa taaatcaatc agcagacctt
    13981 ttgtatcagt gtaagattta ccttccaaat caattgccca accaagtggg gtttctttct
    14041 cttcgcgagc caactgttcc acttttcctc tttgggaaat ggaagtggca gcatcgtata
    14101 aaaaaggata gggaagatca gtaggagctc caaaacagat aggattcgtt cccaaaatgg
    14161 gagaaacact atttgtagga caaatagaag ggcgggcatt ggaaaaaacc aacccaatca
    14221 tattttgttt aattgccatt tctgcataat aaccacaaat cccaaaatga gtggaattac
    14281 gaactgccac agctcccaga ccatattttt cggctttatc aattgccgtt tgcattgctt
    14341 tttggctaat tacttgtccc attccattat ttccatccca aacagcggtg gcaaatttat
    14401 cgcggattac ctcaatgtta gttatgggat tttgaatttt agcttgaatt ctatcataat
    14461 acatttttag tcgtccaact ccatgtgatt caatcccttt taagtcacta gcgattaata
    14521 catcactaca gatttgggca tccgtttggg gcacaccgag ttttataaaa acctcttgca
    14581 taaaattatg cagaacatca acaggtaagt acctcattga tcagacctcc ttatagttct
    14641 ttgtagatta cagctttcaa tggcggaaaa ccattaaaat tcacgctcga ataactctga
    14701 gtgtaagcac cggtcgtaaa aatatacact ttgtcacctg gaactgtccc ttccggcata
    14761 cgataatgga aatgttcata taaaatatcc atactatcac aagtaggacc agctaaaatt
    14821 atctcttctg ccatacctat ttgtggaaaa aatataggga attttatgga ttcatctata
    14881 gtctcaatca aaccaccaaa tttccctata tctaaataga cccattgata aaggttgttt
    14941 ttggatttac gagatatatt aactacttcg gaaacgatca caccagcatc agcaactaga
    15001 gaacgacctg gttctaaaat aatatcaggc aggttttctc caaagtcttc ttgaataaaa
    15061 cgctgaattt cagtagcata ttcttcaata gaaaatgttg ggtcaacata attagcagga
    15121 aaaccacctc cgatattaat catttgtagc tctattcctt cttcagcaac agcatcaaaa
    15181 agatacttac atcgagctat agcatcgtcc cactgaccaa tatctctttg ctgagaacca
    15241 acatgaaaag aaatacccca gggttgcaaa ttcatactgg cagccttcaa tattaagtga
    15301 taaatagtat caggatgcga accaaacttt ctggaaagtg gccaatctgc acctgtccct
    15361 tcagttaaaa tccggaataa tatttttgaa ccgggagcgt atttggccag attctcaaga
    15421 tcaatcatac tatcagtagc aaaaaaatct actcctttat cgtggaagta ctttatatct
    15481 tcaggttttt ttattgtatt gccaaaactt aaacgtgatg gttcaattcc taaccggagc
    15541 atttgatcaa gttcataacg ggaagcaaga tcaaaattcg aacctaaccc tgctagaaga
    15601 agaataactt catccattgg gtttgctttt actgcataga aaactttata atttggcata
    15661 gttgcctgaa gttccaaata cttgtttcga attgtatcca ggtcaataat taaacatgga
    15721 gtaggaatat cctccgcaaa aatcttaatt ttttcaaacc gcttttcatc cataaagcgg
    15781 ccgatattga aagaataagg ttctttatac aatgtcttgt cctcttatgt gttagtaagt
    15841 tgtcccttaa aatcagcaag gcaattcgtg tcaaataaaa attagttggc attttgacag
    15901 tttgcattac tttaaaatat aataagcttg ttgaaacgaa aggcaagaaa aaacctctgt
    15961 gacagtagga acctgcaaag agattaattc agctatgacc gcttttggct atagagttat
    16021 tggcaactat atgtttaaaa agtatattgc caaattagct gttggatacc ctaaaatgat
    16081 taactacaag aaagaagatt tatctatgcc taaagaattc atatattgca gaatagctga
    16141 agttctttca gaatgcatta aatttattgt ccagtataaa cg
//
LOCUS       DAOSTH010000035        15824 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_3812_length_15824_cov_10.804934, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000035 DAOSTH010000000
VERSION     DAOSTH010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 15824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15824
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3812_length_15824_cov_10.804934"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="PLB65_05365"
     CDS             <1..542
                     /locus_tag="PLB65_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HOQ77992.1"
                     /translation="ILFCETLRMPGKGNIILTGLLGDVMKESARIAVSYLKANHSTYM
                     IPPKLFETGDIHIHFPAGAIPKDGPSAGLTLTVALASLFTGQKVRHDIAMTGEITLQG
                     KVLAIGGLKEKLLAARRAGIKRVVIPEENRETLSDFPMDILEGMEITYVKEIYEAIKI
                     LLIPNVEVVEPKRKQKRTA"
     gene            545..1990
                     /locus_tag="PLB65_05370"
     CDS             545..1990
                     /locus_tag="PLB65_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ77993.1"
                     /translation="MEKYPNINILLKVPEKYLPKAEFVLRTYCYILRLNPTFFYGKHF
                     EGAHLYYGVPTKYDYPLKIYFNIDTADFFEKRELYPLEKVDFCKFKNEHIPFLFSRDG
                     AIFSFADESCSFRKDIIASGFYFLTCWHEYILNYYGHSKERIDYKQSLQYRWDFTEIP
                     VVDVYCQMLLYAMEIYCPQFIREISWAEKKRFAVSLSHDIDYWDYWGGSAKVDVFKYN
                     LKTFLKRPLNATYKIGGHLWHKNLIYNPWKTMRTLVRRETEREVRSTWFLLARTDFPD
                     KRQNYIGDVKARVQIMDLLAEQDIGLHGSPQSAFDPLYLAEELSNLRELGFNPTGFRT
                     HYLHFNYQQSFSILEDAGIKYDSTLGYWEHIGFRAGISFPFYPFNIAENRPFRVLEIP
                     LIVMDTTLHSPKAMNLSYFSAKRSLKRLIDIAEKYQSHLSLLWHNTTFDPIDYPFWGK
                     LYWDVIDYALKNQGWVTSLKDIYEEWVTLSY"
     gene            2003..2719
                     /locus_tag="PLB65_05375"
     CDS             2003..2719
                     /locus_tag="PLB65_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOQ77994.1"
                     /translation="MKSLLWQIAFCLFLLFYLVIYVFYFIAKIILPKRVSQYLLKKIS
                     RSWARIVILSTGSKVRVSGKENLPRVNNICFVSNHQGLFDIPLILGFLGIHTGFVAKQ
                     ELFKIPILSQWMKEIPCTFIDRRNPRKAMETFKKSAELIKKGNPLVIFPEGTRSRSDL
                     VGKFHLGSLRLPIMSEAVIVPLAIKGSWRIYEIDKKIHSAEVKLQILPPILPTDEIYK
                     DKMALASYLHSQISQAVQEM"
     gene            complement(3065..5071)
                     /locus_tag="PLB65_05380"
     CDS             complement(3065..5071)
                     /locus_tag="PLB65_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ77995.1"
                     /translation="MRIKSITIIILACLAFIWTSLSADDVIIGIGNQQERIPLDFFYK
                     SSLYECLYYQDELGFSQGTISNIAFYNNFVTNLANKPTKIWLGTTNSIDLNTGWIPST
                     QLNLVFDGTVNYPAGNHIVNIELDSLFQYTGGSLVMMVLRPMDNGYFSSSDKFYCQTD
                     TVNRARKAYNDNELLDPANPPVSANPNGQFPKVKFTYTSQNIATDLACPSINVNSSVT
                     LGTVEEVQVQINNYGYVDQNNYTVRVRNEDGDVLVSAPGNPIGAGQVINQTLLWVPST
                     PGNYSICAEVLVTGDEDLTNNISPSVRVSAFEEGYILFTVGIGNLSGYLPLDFTWPTS
                     LFETIIYAPEMESEGVITHLTFYNSFVQSIANKPVIIWLGETDLVNLPLNWIPSTQLT
                     MVYYGNMSFPVGQNEIVFPLSPQYHYQGQNLVLMVQRPFDYQVFNTDNKFKMQSGAIQ
                     RTKYIYSDQQEHNPAAPPPFAFDSYQYPQTGFIMRPANSGYLRGTVKNGTALVSEASV
                     TADGIQTTYTSSLGTYSLLLSSGLHTVTASCTGLHTVTKQVNIIAGEVTNENFNLDGS
                     PVNDQETPQLTTSFKGIYPNPFNPEATLSFSIDKNNTPVTIELYNIKGQKMKTLANAI
                     YDKGEFQLKFSAQEPNSNELASGIYFIRLFTPGYNKTVKALLLK"
     gene            complement(5155..5532)
                     /locus_tag="PLB65_05385"
     CDS             complement(5155..5532)
                     /locus_tag="PLB65_05385"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOQ77996.1"
                     /translation="MIIGIGCDIIEVQRIQKAIENNPRFCDKLFTAAEIEYCSRKANS
                     YQSYAVRFAAKEAVMKALGTGWSETVTWLDIEIIVSKKGLPSVVLSGEAKEIADNLLI
                     NNIHLSLSHEKNYAIAFVIMEAV"
     gene            complement(5529..5996)
                     /locus_tag="PLB65_05390"
     CDS             complement(5529..5996)
                     /locus_tag="PLB65_05390"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HOQ77997.1"
                     /translation="MDTRLFYNSPNGFFHLVIEIFEETIISINFCSNQISSQPVEPLE
                     KEICHQFDLYFESKSKDFDLPFFATGTVFQLLVWEEVMKIPYGSTISYKDLAERIGRS
                     NSARAVGRALNQNPIPILIPCHRVIGSNKDLKGYGGGLPIKKRLLELEGSVTL"
     gene            complement(6214..6567)
                     /gene="rplS"
                     /locus_tag="PLB65_05395"
     CDS             complement(6214..6567)
                     /gene="rplS"
                     /locus_tag="PLB65_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOQ77998.1"
                     /translation="MDILQQVGRDQIRTDLPDYRVGDTVKVHYKIKEGNKERIQVFQG
                     IVIQKKGAGVSKTFTVRKVSSGVGVERIFPQNSPNIDKLEIVRHGQVRRAKLFYLRSA
                     QGKAGRIKERRRFTV"
     gene            complement(6788..7459)
                     /gene="trmD"
                     /locus_tag="PLB65_05400"
     CDS             complement(6788..7459)
                     /gene="trmD"
                     /locus_tag="PLB65_05400"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOQ77999.1"
                     /translation="MIFEVLSLFPDFFSGFLTSSIIFRAIENELLQVKLTDYRTYSKN
                     RHHQVDDYPYGGFPGMVIQAQPIYDALQELLLEGNAPVIYFTPQGRSLNQQILQNYTS
                     YERIILLCGHYKEIDQRVRDLCISDEISIGDYVLSGGEIPALVFMDGISRLLPGVLSD
                     IESANSDSFSSGGLGFPCYTRPDIFMGLKVPDILLSGNHKLIKQWASEQGILLTRTRR
                     PDLIK"
     gene            complement(7456..7986)
                     /locus_tag="PLB65_05405"
     CDS             complement(7456..7986)
                     /locus_tag="PLB65_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78000.1"
                     /translation="MNHPFLTGIGKLGRQDADGFHPVMIKPEFIAVFSELEELYLIFN
                     SDRVFYVTISERKIKDKKTWIKLKEDGIAEEQYLHKDVVIAIDAQDTESKGTSLDYLL
                     GYQAIFADEEFGIIEDFFFNGAQQVLQIVDKNKIEYLVPFVDYYIDTILNNPGCVILK
                     NALELIAFYRAEAKSK"
     gene            complement(7987..8217)
                     /locus_tag="PLB65_05410"
     CDS             complement(7987..8217)
                     /locus_tag="PLB65_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HOQ78001.1"
                     /translation="MKELIEFIVKALVDDPSEVNITEITGDKITLYELRVSKADIGKV
                     IGKRGRTASAIRTIINAVSTKQGKRAELEIIE"
     gene            complement(8239..8532)
                     /gene="rpsP"
                     /locus_tag="PLB65_05415"
     CDS             complement(8239..8532)
                     /gene="rpsP"
                     /locus_tag="PLB65_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOQ78002.1"
                     /translation="MVKLRLRRMGANDQPFYRIVAVDSREKRDGKYIECVGWYDPKPD
                     PSKINIDTERAIYWLSVGAQPSDTVSSLLRKAGILQIWHERKLANQKTADAEK"
     gene            complement(8634..9956)
                     /locus_tag="PLB65_05420"
     CDS             complement(8634..9956)
                     /locus_tag="PLB65_05420"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HOQ78003.1"
                     /translation="MKIAVIGSGYVGLTTGACFAEMGNTVICVDKDEAKIKLLNSGKV
                     PIFEPGLQEMISRNMAAKRIIFTLDLKYAVQESHIIFIAVGTPPGEDGSADLQYVICA
                     AEEISGCMNEPKIIVNKSTVPVGTADIVRAKIQEVLDSRGLELPFDVVSNPEFLKEGA
                     AIDDFMKPDRVVIGTDNPEAGEIMRTLYEPFCRTNDRVLIMNIRSAEMAKYAANAFLA
                     TKISFINEISRLCDAYEADISEVRNGMCSDSRIGYKFIFPGVGYGGSCFPKDIKALIY
                     MSQKVGYNPLILTAVEEVNNYQKRVLVEKVVRHFGSNLSGKTFAVWGLAFKPQTDDMR
                     EAPSIVIINELLAMGAKIKAYDPVAMDEAKKIFGNNNVVTLCADEYETLKDTDAMLLI
                     TEWHQFRYPDFERMSKIMRQKVIFDGRNQYNPKAIKEMGFSYYGIGRR"
     gene            complement(9953..10885)
                     /locus_tag="PLB65_05425"
     CDS             complement(9953..10885)
                     /locus_tag="PLB65_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOQ78004.1"
                     /translation="MKILITGGAGFIGSNVADAYLKAGHKVIIVDNLVTGKRRNINPE
                     AVFYEMDICDENLSQIFDKEKPDIVNHHAAQISVPLSVENPLMDITSNVYGWVNVLQN
                     CINSGVKKVIYISSGGAIYGEAEEYPTTETYNPKPLSIYAINKSVGEDYLYFYRYQYG
                     LEYTILRYANVYGPRQISSGEAGVVSLFTEKLLKGDIPTVYRYDGEPDGMIRDYVYVG
                     DVVKANLLALEIGEGDAFNIGTCLETKTRDLYWEIANQLGVNEEPHYGVARKGDLHRS
                     LLSYEKAKRVLGWEPKIGLSEGISQVIKYIKELK"
     gene            complement(10885..11457)
                     /locus_tag="PLB65_05430"
     CDS             complement(10885..11457)
                     /locus_tag="PLB65_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HOQ78005.1"
                     /translation="MATHKPVYIDELYGELELFTDDRNWVVVHTKPRCEKKVADYAKK
                     NQITYYLPQYTDKRVYQRRKVDVDKILFPSYIFVGIDFKNKQELLISGYTVNFLKVNA
                     QKELVDELRAIYYGRQKKVEMQPGLWLEKGLEVEIVNGPLKGIHGVVENQSKLTEVRL
                     QVNILRQAVLVKVSTEDIKIIGEYEVVEID"
     gene            complement(11461..12861)
                     /gene="ahcY"
                     /locus_tag="PLB65_05435"
     CDS             complement(11461..12861)
                     /gene="ahcY"
                     /locus_tag="PLB65_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOQ78006.1"
                     /translation="MDYKIADINLADWGRKEIDLAEKEMPGLAALKESYSERKPLAGA
                     KITGSLHMTIQTAVLIETLVELGAQVRWASCNVFSTQDNAAAAIAKRGIPVFAWKGES
                     LNEYWWCTWQALSWEDGPDLIVDDGGDATLMVHEGYRLEEIYKQTGKIPEIDAENEEM
                     KIVQELIINDLGKKPEKWHKIVKHLHGVSEETTTGVHRLYQRRDEGTLLFPAINVNDS
                     VTKSKFDNLYGCRESLADGIKRGTDIMVAGKVVMVCGYGDVGKGCAQSMRGLGARVVI
                     SEIDPICALQAAMEGYEVNTVENMLEIADIFITATGNCDVIRVDHILKMKNNAIVCNI
                     GHFDNEIQVNKLYEMEGVEKVNIKPQVDLIKLPNDTTIILLSEGRLVNLGNATGHPSF
                     VMSCSFSNQVLAQIELWQNEHNIDVYTLPKQMDEEVARLHLPKLGVKLTQLSKKQAEY
                     INVPIEGPYKPEYYRY"
     gene            complement(12890..15109)
                     /gene="pnp"
                     /locus_tag="PLB65_05440"
     CDS             complement(12890..15109)
                     /gene="pnp"
                     /locus_tag="PLB65_05440"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOQ78007.1"
                     /translation="MHNFNITSRETDFCGRKLIVETGKMAKQANGSVFIQYGESAILV
                     TATMSKEPVENQDFFPLTVDYIEKMYAAGKIPGGFFKRESKPSTDATLMARVIDRSIR
                     PLFPEGFRNQVHIVVTVLSFDGVTDPSTMGIFGASLALSISDIPFNGPIAGATVGYID
                     DQFVVNPHFDEIESKSKLNLTVAGSDDSIVMIESAASVLSEETMLEAVFIGHNEIKKL
                     CSLQKDFISACGKEKVEVVIDSIPEEIMNRVENAFGLKIAEAAVIFGKQERQDAFDAA
                     EAEMVEMFTAIEKDAWEEKEKYYQKAFEELIRRYVRDSILKKHHRVDSRGTDDLRDIT
                     CEIDILPRVHGSALFTRGETQSLGTLTLGGGIDEQMIDGLENEYKKSFFLHYNFPPYS
                     VGEAGKMGPTGRRELGHGNLAERALKAVLPDKDVFPYTIRVVADTLESNGSSSMASVC
                     SGCLALMAGGVPIKAPVAGIAMGLIMEDKDYVVLTDIMGLEDHLGDMDFKCAGTREGI
                     TAMQMDIKISGITKEIMQIALNKAKIARLRILDIMSECISEPRSDLAPWAPRIESFKV
                     PIDKIGEIIGPGGKMIKSIIEACQVQIDIQDDGTVRILSPDKASITKAKEMIRGIAVD
                     PVAGDMFEGEVTRIEPYGIFVKILGGAKEGMVHISEMHTGRINHPLDMVKIGDKVQVK
                     ALGMDKGKLSLSMKGVAGNPEPNPNSSRENAQKYHNQNYNNRNKGRDSDRNDYRRRR"
     gene            complement(15414..15683)
                     /gene="rpsO"
                     /locus_tag="PLB65_05445"
     CDS             complement(15414..15683)
                     /gene="rpsO"
                     /locus_tag="PLB65_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOQ78008.1"
                     /translation="MPLTPEAKQAIMAEFKLHESDTGSPEVQVALLTQRIKDITEHLK
                     EHPKDFSTRRGLLKLIGQRRRLLDYLKKKDITRYRNLIKALGIRK"
     gene            complement(15774..>15824)
                     /gene="ssrA"
                     /locus_tag="PLB65_05450"
     tmRNA           complement(15774..>15824)
                     /gene="ssrA"
                     /locus_tag="PLB65_05450"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         4816 a         3201 c         2588 g         5219 t
ORIGIN      
        1 aaatactatt ttgtgaaaca ctccggatgc caggaaaagg aaatattatt ttaactggtc
       61 ttctgggaga tgtgatgaaa gaatcagctc gtattgccgt cagctatcta aaagctaatc
      121 actccactta tatgatacct cctaaacttt ttgaaacagg cgatatacat attcattttc
      181 ctgctggagc aatccctaag gatggacctt cggctggttt aacacttacc gtagctttag
      241 cttctctttt cacgggtcag aaagttcgtc atgatattgc tatgacagga gaaattaccc
      301 tgcagggaaa agttttggca attggaggac taaaagaaaa acttttagcc gctcgtagag
      361 ctggaataaa gagagtagtg attccagaag aaaatcgcga aaccctttcc gattttccaa
      421 tggatattct ggaaggaatg gaaattactt atgtgaagga aatttacgaa gcaatcaaga
      481 tcttactgat tcccaatgtt gaggtagttg aaccgaagcg caaacagaaa aggactgctt
      541 aactttggaa aaatacccta atatcaatat attattgaag gttccagaaa aatatctgcc
      601 taaagcagaa tttgttttgc gcacctactg ctatattttg cgcttaaatc ctaccttctt
      661 ttatggtaag cattttgaag gagcacattt atattatggc gttcctacca aatatgatta
      721 tccattgaaa atatatttca acatagatac agcggatttc tttgaaaaga gggaattgta
      781 tcctttggaa aaagtggatt tttgcaagtt caaaaatgag catattccct ttttattttc
      841 tcgggatgga gctatttttt cttttgctga tgagagctgt tccttccgta aagatattat
      901 tgccagcggg ttttattttt taacctgctg gcatgaatat atcctaaact actatggaca
      961 ttcaaaagaa aggatagatt ataaacaaag tttgcaatac cggtgggatt ttactgaaat
     1021 acctgtagtt gatgtttatt gccaaatgct cttatatgca atggagatat actgtccaca
     1081 atttattaga gaaattagct gggcagagaa aaaacgattt gccgtatctt tatcacacga
     1141 tattgactat tgggattatt ggggtgggtc tgccaaagtt gatgtcttta aatataatct
     1201 gaaaacattt ttaaagcgtc ctctgaatgc cacttataaa attggtggac atttatggca
     1261 taaaaacctg atatataatc catggaaaac aatgcgcact ttagtaagaa gggaaaccga
     1321 aagagaagtt cgttcaacct ggttcttatt agcgcgtacc gatttccctg ataagaggca
     1381 aaattatatc ggtgatgtaa aagcaagagt acaaattatg gacttactgg ctgaacaaga
     1441 tattggttta catggaagtc cccaatcagc atttgatcca ttatatttag cagaggagct
     1501 ttccaatcta cgagaattag gttttaatcc tactggcttc agaactcact atctacattt
     1561 caactatcaa cagagtttct ccattttgga agatgctgga ataaaatatg attctacttt
     1621 aggatattgg gagcatattg gttttcgtgc tgggatatct tttccttttt atccattcaa
     1681 catagccgaa aatcgaccct ttcgagtttt ggaaattcca cttattgtaa tggatacaac
     1741 tcttcattcg cccaaagcaa tgaatttgag ttatttttcc gctaagcgtt ctttaaaacg
     1801 cttgattgat atcgcagaaa aataccagtc ccatctttca ttactttggc ataatacaac
     1861 ttttgatcct atagattatc cattttgggg taaattatac tgggatgtaa ttgattatgc
     1921 tctaaaaaac cagggctggg ttacatctct taaagacatc tatgaagaat gggtaaccct
     1981 tagttattag aaacatacta ttatgaaatc gctgctttgg caaattgcgt tctgtttatt
     2041 tttattgttc tatctggtga tttatgtatt ctattttatc gccaagatta tattgcccaa
     2101 aagggtttcc cagtacctgc taaaaaaaat atctcgctct tgggcaagaa tagtcatctt
     2161 atctacaggt agtaaagttc gtgtttcagg aaaagaaaat ctacctagag ttaataatat
     2221 ttgctttgtc agcaatcatc agggcttgtt tgatattccc cttattcttg gttttttagg
     2281 cattcatact ggttttgttg caaaacaaga attatttaaa attccgatac tgagccaatg
     2341 gatgaaagaa attccctgca cttttattga cagaagaaat ccccgtaagg caatggaaac
     2401 ttttaaaaaa agtgctgaac tcattaaaaa aggtaatccg ctggttattt ttccagaagg
     2461 aactcgtagc aggagtgatc ttgttggtaa gtttcatttg ggtagtctaa gacttcctat
     2521 tatgtcagaa gcagttattg taccattagc aatcaaagga agctggcgaa tatacgaaat
     2581 tgataagaag attcattctg ctgaagtgaa gttacaaata cttcctccaa ttctaccaac
     2641 cgatgaaata tacaaagata aaatggcact tgccagttat ctccattccc aaatttccca
     2701 agctgttcag gaaatgtaaa aaaatattcc agtatctttc agtatattct tcagatggtt
     2761 aatgttgtat acttattatg ttctataatc caattattta atctatttaa ttcagttata
     2821 tatattctat acctcacatt tcatatgtaa agtctattca gaaatctcgt ttgcctactg
     2881 cttactatct accgtggaga taccgtggag ctactgggca atatgccaag gaatactacc
     2941 tcagcttcac cgtatatcat taaggaagga ttaacttcag ccgtattact catctacttg
     3001 ataattgcgt ttgaataaat gctacacagt gatcaataga ggattagttg ctatatattg
     3061 gttcttactt cagcaataga gctttcacag ttttattgta tccgggagta aaaagacgaa
     3121 taaagtagat tccggatgca agttcattac tatttggctc ctgtgctgaa aatttaagtt
     3181 gaaattcacc cttatcataa attgcatttg ccagggtttt catcttttga cccttgatgt
     3241 tatacaattc tatcgtaaca ggagtattat tcttgtcaat gctgaaggaa agagttgcct
     3301 ctggattaaa cggattcgga taaatccctt tgaatgaagt tgtaagctga ggtgtttcct
     3361 gatcgttaac cggacttcca tccaggttaa aattctcatt agtaacttcc cctgcaataa
     3421 tattcacttg tttggtaaca gtgtgcaatc cagtacagga tgctgtaacg gtatgaagcc
     3481 cactgcttag aagtaatgaa tatgttccca gagaagatgt ataggtagtt tgaatgccat
     3541 ccgcagtaac tgatgcctcg gaaactaagg cagttccgtt tttaacagtg cctcttaaat
     3601 aaccgctatt cgctggacgc ataataaatc cagtttgggg gtattgatat gaatcaaatg
     3661 caaaaggagg tggagctgct ggattatgtt cctgttggtc actataaata tatttggtgc
     3721 gttgtatcgc tcctgactgc attttaaatt tattatcggt attaaaaacc tgataatcaa
     3781 atggacgctg aaccattaga actaggtttt gaccttggta atgatattgt ggagaaagcg
     3841 gaaaaactat ttcattctgc cctacaggaa aagacatatt tccgtaatat accattgtaa
     3901 gctgtgtgct tggaatccaa ttcagaggta gattcacaag gtctgtttcc cccagccaaa
     3961 taataactgg tttattggca atgctttgaa caaagctgtt gtaaaatgtc agatgagtaa
     4021 tcacaccttc cgattccatt tccggagcat aaatgatagt ttcaaacaaa cttgttggcc
     4081 aggtaaaatc taacggtaaa tatccagaga gattgcctat tcctacagta aagagaatat
     4141 aaccttcttc aaaagctgaa actcgaacac ttggtgagat attattagtt aaatcctcat
     4201 caccagtaac aagaacttca gcacatatag aataatttcc gggagtagaa ggaacccaca
     4261 ataaggtctg atttattact tgacctgctc ctataggatt tccaggagca gaaactaaaa
     4321 catctccatc ttcatttctt acccttacag tgtaattatt ctgatctaca tagccataat
     4381 tattgatttg cacttgcact tcttcaactg tgcccaaagt tacagatgaa ttaacattta
     4441 ttgatggaca agccagatct gtagcaatgt tttgactggt ataagtaaat ttaactttag
     4501 gaaattgacc attaggatta gcagaaacag gaggattagc tggatcaagt aattcattat
     4561 cattatatgc tttacgcgct cgattaactg tatcggtttg acagtaaaat ttgtcactac
     4621 tggaaaaata accattatcc ataggacgta aaaccatcat aactaatgag ccaccagtat
     4681 attgaaatag cgaatccagt tctatattaa ctatatgatt acccgcagga taattaactg
     4741 tcccatcaaa aactaagttt aattgcgtgg aaggaatcca gcccgtattg agatctatag
     4801 aatttgtagt tcctaaccat atttttgtag gcttattggc aagattggtt acaaaattat
     4861 tgtaaaaagc aatattagaa atggtccctt gtgaaaaacc aagttcatcc tgatagtaga
     4921 ggcattcata taaagaactc ttataaaaaa aatctaaagg aattcgttct tgttgattgc
     4981 ctataccgat aataacatca tcggcactca aacttgtcca tataaaagct aagcaagcca
     5041 atattatgat tgttatactc ttaatacgca tcttatggca tactcctaat tattctcaaa
     5101 cattagtata cttcttaaga atgaagcaac tttcgaaccg aacttacaga taatttatac
     5161 tgcttccata ataacgaaag ctatggcata attcttttca tgcgacaaac tgagatgaat
     5221 attatttatc agtagattat cagctatttc tttagcctca ccagaaagaa ctactgaagg
     5281 caaacccttt ttagacacga tgatttcaat atccaaccaa gttactgttt cactccaacc
     5341 ggttcccaag gctttcataa cagcttcttt tgctgcaaac ctaactgcat atgactgata
     5401 tgaatttgct tttcgagaac agtattcaat ttcggcagca gtaaacaatt tatcacaaaa
     5461 acggggatta ttctctattg ccttttgaat tctttgaacc tctataatat cacatccaat
     5521 ccctataatc ataatgtaac acttccttca agttcaagca acctcttttt tataggtaac
     5581 cctcccccat aaccttttag atctttattg gaaccaatta cccgatgaca aggaataaga
     5641 atgggaatag gattttgatt tagagctcta cctacagctc tggcagaatt cgatctgcca
     5701 atcctttcag ctaaatcttt atatgatatg gtagatccat atggtatttt cataacttct
     5761 tcccaaacta agagttgaaa aacagttccc gtcgcaaaaa aaggaagatc aaaatctttg
     5821 ctcttacttt caaaatagag atcaaactga tgacagattt ctttttccag gggttcaaca
     5881 ggttggctgg aaatttgatt actgcagaaa tttatactga taatggtctc ttcaaatatt
     5941 tcaattacga ggtggaaaaa accattggga ctgttataaa ataatctggt atccatatat
     6001 atctcctttt gggctgtggc tgttccaata tttagaatca aaaaagaagg ttgagatcat
     6061 tatcaccgga ccagcatttc cgtgatgcta tactaaaatt cagcaatttc aatatagtat
     6121 tttaaagtag ggagctgaaa tcggtattgt attaaaaaat aaagcccctt ccaattaata
     6181 atgaaggggc taagattgag tttctttaag taactataca gtgaatcttc ttctttcttt
     6241 gattcttcca gctttccctt gagcgctacg aagataaaat agtttggctc tacgtacttg
     6301 accatgccga actatttcga gcttatcaat attcggtgaa ttttggggaa atatcctctc
     6361 cacaccaaca ccactggaaa ctttacgtac agtgaaagtt tttgaaacgc cagcgccttt
     6421 tttttgaata acgataccct gaaagacctg aatgcgttcc ttatttcctt ctttaatttt
     6481 ataatggacc tttacggtat cgccaacgcg ataatccggt aagtctgttc tgatttggtc
     6541 tctgccaact tgttgcagaa tatccatttg ttgggttcct cccatatata tactcgggag
     6601 aaaaaaacct attgtgatca agggagaacg gactaaaaat aaattctcta taaaccgccc
     6661 tgttatttct atcctcatta gatgcattgc tacaataaga taaggcccac agagatataa
     6721 tttcccttca ggcatattcc ttgcaatcac gcataagttc aggtttcttt cttcctgctt
     6781 gcttgtctta ctttatcaaa tctggtctgc gagttcttgt aagtaatata ccctgttcac
     6841 ttgcccattg tttgatcaat ttatggttgc cactaagtaa aatatccgga acttttaaac
     6901 ccataaatat gtcaggtcgt gtataacagg gaaaacccaa tcccccactg ctgaaagaat
     6961 cactatttgc actttctata tcacttagta ctcctggtag caatctgcta attccatcca
     7021 taaaaacaag tgctggaatt tctcctccgc ttaaaacata atctcctata gaaatttcat
     7081 ccgaaataca aaggtctcga actctttgat caatttcttt gtagtgacca cagaggagga
     7141 ttatccgttc ataggatgta tagttttgca gtatctgctg atttaaactt ctaccttgag
     7201 gagtaaaata tataactgga gcatttcctt ccagcaaaag ttcttgcaaa gcatcataaa
     7261 ttggctgtgc ttgaataacc ataccgggaa agccaccata gggataatca tctacttgat
     7321 gatgtctatt tttagaataa gtgcgataat ccgtcagttt cacctgtagc agctcatttt
     7381 ctatcgctct aaatataatg ctgcttgtta aaaagccgga aaagaagtcc ggaaaaagag
     7441 aaagtacttc aaagatcatt tcgattttgc ttctgcccga taaaaggcaa tgagctctaa
     7501 tgcatttttc aaaattacac agccaggatt gttaaggatc gtatctatat aataatcaac
     7561 aaaaggaact agatattcaa ttttattctt atccacaatc tgtaaaacct gctgagcgcc
     7621 attgaaaaag aagtcctcta ttattccaaa ttcttcatct gcaaaaatag cttgataacc
     7681 taaaagataa tccaacgatg taccttttga ttcagtatct tgtgcatcaa tagcaattac
     7741 tacatcttta tgtagatatt gctcttcagc gattccatct tctttcagtt taatccaggt
     7801 ttttttatcc tttatttttc tctcactgat agttacataa aacactctat cgctgttaaa
     7861 gatcaaatac aattcttcaa gctcagaaaa cacagctatg aattctggct tgatcatcac
     7921 aggatgaaag ccatctgcat cctgcctacc cagtttgcct attccagtta aaaaaggatg
     7981 attcatttac tcgataattt cgagctcggc acgcttcccc tgttttgtac taacggcatt
     8041 aataattgtt ctgatggcac ttgcggttct accccgtttg ccaataacct ttccaatgtc
     8101 agctttcgaa accctaagct cataaagggt tattttgtcg ccagtgatct cggtaatatt
     8161 cacttccgaa ggatcatcaa ccagagcttt aacaatgaat tcaatcagtt ctttcatttt
     8221 tcacctcagc taatctattt atttttctgc atccgcagtt ttctggtttg caagtttccg
     8281 ttcgtgccaa atttgaagta tacctgcttt tcttaacaat gaagaaacag tatcagatgg
     8341 ttgtgcacca acacttaacc agtaaatggc acgctctgta tctatgttta ttttggaagg
     8401 gtcgggtttt ggatcatacc aaccaacgca ttcaatatat tttccatctc gtttttcgcg
     8461 agaatcaact gccactatac gatagaaggg ttgatcgttt gctcccatcc ttcttaatct
     8521 cagttttacc attagttctc cttctaatta taacaatatt ttaccaagtt ttgatggaag
     8581 gactttccgt caagacaatt tttttatctg ctgctaataa atatttatta ttactatctt
     8641 ctaccaatcc cataatagct aaaacccatc tctttaatag ctttgggatt atattgatta
     8701 cgcccgtcaa agataacttt ttgacgcata attttactca tcctttcgaa atctggataa
     8761 cggaactgat gccattctgt aattagtaac attgcatctg tatctttaag ggtttcatat
     8821 tcatcagcac aaagagttac aacattattg ttcccgaata tctttttggc ttcgtccata
     8881 gctacaggat cataggcctt tatttttgct cccatagcaa gaagttcatt gatgattact
     8941 atggaaggtg cttctcgcat atcatctgtt tgtggtttaa aagctaaacc ccaaactgca
     9001 aaggtttttc cagataaatt actaccaaaa tgacgaacta ctttttccac taaaaccctc
     9061 ttctgatagt tgttaacttc ttccaccgct gttaagataa gaggattgta acccactttt
     9121 tgagacatat aaataagggc ttttatgtct ttggggaaac aactgcctcc ataaccaact
     9181 ccaggaaaaa tgaatttgta accgattcga gaatcactac acattccatt tcgaacttct
     9241 gatatgtctg cttcataagc atcgcaaaga cgagatattt cgttaataaa tgatatcttt
     9301 gtagccaaaa aagcattagc tgcatatttt gccatttcag ctgaacgaat attcataatc
     9361 aaaaccctat cattagttct gcaaaacggt tcgtataagg ttcgcataat ttctccagct
     9421 tcaggattat ctgtaccgat aacaactcta tcaggtttca taaaatcatc tattgcagcg
     9481 ccttctttca aaaattcagg attggataca acatcaaaag gtagttccaa accacgagaa
     9541 tcgagaactt cttgaatttt ggcacgcact atatctgcag tcccaactgg aacagtggat
     9601 ttattaacta tgattttggg ctcattcata caaccagaaa tttcttcagc agcacaaatt
     9661 acatattgta aatcagcaga accatcttct ccaggaggag ttccaactgc aataaagata
     9721 atatgtgatt cttgaacagc atatttgagg tctaaagtaa aaataatacg ttttgcagcc
     9781 atattgcgac tgatcatttc ttgcaaacct ggctcaaata ttggaacttt acccgaattg
     9841 agtaacttta ttttagcttc atccttatct acacagataa ccgtgttccc catttctgca
     9901 aaacaggcac ctgtagttaa acctacatag ccactaccaa taactgcaat tttcatttga
     9961 gctccttaat gtacttaata acttgtgaaa taccttcact aaggcctatt ttgggttccc
    10021 aacccaatac tctttttgct ttttcatatg aaagtaagga acggtgtaaa tccccttttc
    10081 gagcaacgcc gtaatgtggt tcttcgttta cacctaactg gttagcaatt tcccaatata
    10141 aatctctggt tttggtttca aggcaagttc caatattaaa tgcatctccc tcacctattt
    10201 ccaaagcaag taaattagct ttaactacat ctcctacata tacataatcc ctaatcattc
    10261 catctggttc accatcataa cgataaactg taggtatatc acctttcaat aatttttcag
    10321 taaaaagaga tacaacacca gcttctccac tggaaatttg acggggacca taaacattgg
    10381 cataacgtaa aatagtatat tcaagcccat attgataacg atagaaatat aaataatcct
    10441 cccctacact tttattaata gcataaatgg ataggggctt aggattatat gtttctgtag
    10501 taggatattc ttctgcttcg ccataaatag cacctccaga ggagatatag attacttttt
    10561 ttaccccact gtttatgcaa ttctgaagta cattcaccca accatataca ttgcttgtga
    10621 tatccataag aggattttca actgaaagtg gaacactaat ctgcgctgca tggtgattta
    10681 caatatctgg tttctcctta tcaaagattt gggataagtt ttcatcacaa atatccatct
    10741 cgtaaaatac ggcttcagga tttatgtttc ttcgtttacc agttactaaa ttatccacaa
    10801 ttatcacttt atgccctgct tttagatatg catctgctac atttgatcca ataaaacctg
    10861 ctccacctgt gatcaatatt ttcattaatc tatctccaca acttcatatt caccgataat
    10921 cttaatatct tcagtgctaa ctttaaccaa aactgcctgc cttaagatat ttacttgtaa
    10981 acgcacttcc gtgagcttag attggttttc cactacaccg tgaatccctt ttagaggacc
    11041 gtttacaatt tctacttcca atcctttttc aagccaaagt ccaggttgca tttccacctt
    11101 tttttgccta ccataataga ttgccctcaa ttcatctact agttcttttt gagcattcac
    11161 tttcaggaaa ttgacagtat aaccagatat cagtaattcc tgcttgtttt tgaaatcaat
    11221 tcccacaaaa atataggagg ggaaaagaat cttatccaca tctaccttgc gcctttgata
    11281 tacacgctta tcggtatatt ggggtagata ataagtgatc tgattctttt tagcgtaatc
    11341 ggcaactttc ttttcacagc gtggtttggt atgcactacc acccaatttc tgtcatctgt
    11401 gaaaagctca agttcaccat ataattcatc tatatataca ggtttgtgtg tagccatatc
    11461 ttaatagcga taatattcag gtttatatgg gccctcaata ggtacattaa tgtattctgc
    11521 ctgctttttt gagagttgag ttaatttcac tcctaacttg ggaagatgta aacgagcaac
    11581 ttcttcatcc atttgtttgg gcaaagtgta cacatcaata ttatgctcat tttgccagag
    11641 ttcaatttgg gctaaaacct gattgctgaa agaacagctc atcacaaatg aaggatgccc
    11701 tgtagcattg ccaagattaa ctaaccgccc ttcagacagt aagataatag ttgtatcatt
    11761 agggagcttg attaaatcta cctgaggttt aatgttaacc ttctccacac cttccatttc
    11821 atagagttta tttacttgaa tttcgttgtc gaagtggcca atattacata caatagcgtt
    11881 attcttcatt tttaaaatat ggtcaacccg aataacatca caattacctg tagcagtaat
    11941 aaagatatca gcaatttcca gcatgttctc aacagtatta acttcataac cttccattgc
    12001 tgcttgtaac gcacaaatag gatcaatttc actaattaca actctggctc ctaaaccacg
    12061 cattgattgt gcgcaacctt ttcctacatc accatatcca caaaccataa caacttttcc
    12121 agcaaccatt atatccgttc ctctctttat tccgtcagcc aaggattcgc ggcatccata
    12181 aagattgtca aatttggatt tggtaacgga atcattaaca ttgatggcag gaaacagtaa
    12241 agttccttca tcccttcttt gataaagacg atgtactcct gttgttgttt cttcactaac
    12301 tccgtgtaag tgttttacaa ttttatgcca tttctccggt ttttttccca aatcgttaat
    12361 aatcaattcc tgcacaattt tcatctcttc attttccgca tcaatttctg gaatctttcc
    12421 tgtttgttta tatatttctt ccaaacggta accttcgtga accatcaaag ttgcatctcc
    12481 accatcatca acgattaaat ctggaccatc ttcccaggat aaagcttgcc aagtacacca
    12541 ccaatattca tttagtgatt cacctttcca agcaaatacg ggaattcctc ttttagcaat
    12601 agcagcagca gcattatcct gagtagaaaa aacattacag cttgcccaac gaacttgagc
    12661 tccaagttct acaagagttt caattaaaac cgcagtttga atagtcatat gtaaactgcc
    12721 agttattttt gctccggcta agggttttct ttcactataa ctttctttca aagctgccag
    12781 accaggcatt tctttttctg ccagatcaat ttctttcctg ccccaatctg ccaggtttat
    12841 atcagcaatt ttgtagtcca ttttttcctc tatcattttt tatttattat taacgacgac
    12901 ggcgatagtc atttctatcg ctatctctac ccttatttct gttgttatag ttttgattat
    12961 gatacttttg agcattctct ctggaagaat taggattggg ttcaggatta ccagctacac
    13021 ctttcatcga taaggagagc ttacctttat ccattcctag agctttaact tgaactttat
    13081 ctccgatttt aaccatatcc agaggatgat ttatccgacc tgtatgcatt tcagagatat
    13141 gtaccattcc ttcttttgct ccgcctagaa ttttaacaaa tattccgtat ggttcaatcc
    13201 gtgtaacttc tccttcaaac atatcgccag ctactggatc aacagcaatt cctcttatca
    13261 tctctttagc ttttgtgata gatgctttat ccggagataa tattctcact gttccatcat
    13321 cctgaatgtc tatctgtacc tgacaagctt caataattga tttgatcatc ttaccaccgg
    13381 gtccaattat ctcaccaatt ttatcaatag gtactttgaa agattcaatt cgaggtgccc
    13441 aaggagcaag atcacttctc ggttcggaaa tacattcaga cataatatcc aatattctca
    13501 aacgtgcaat ctttgcttta ttaagtgcta tctgcataat ttctttggtg attccagata
    13561 ttttaatatc catctgcata gcagttatac cttcacgagt tccggcacac ttgaaatcca
    13621 tatctcctaa gtgatcttcc aaacccataa tatcggttaa aaccacatag tctttatctt
    13681 ccataattaa tcccattgca attcctgcaa ctggagcttt tataggaaca ccaccagcca
    13741 tcaaagctaa gcatccacta caaactgaag ccattgagga tgaaccatta gattccagag
    13801 tatcggctac cacacgaata gtgtagggga aaacatcttt atcaggtaaa acagctttca
    13861 gcgctctttc tgctaaatta ccatggccca gttcacgacg ccccgtaggg cccatcttac
    13921 ctgcttcgcc aacactatag ggcggaaaat tatagtgcag gaaaaagctc tttttgtatt
    13981 cgttttccaa gccatctatc atttgttcat caattccacc acctaaggta agggttccta
    14041 aagattgcgt ttcaccacga gtgaacaaag ctgaaccatg cactctgggt aagatatcta
    14101 tttcacaggt aatatcacgg aggtcatcag ttccgcgact atctactcta tgatgttttt
    14161 ttagtataga atcgcgaaca taacgacgga taagttcctc aaatgctttt tgataatatt
    14221 tctctttttc ttcccaggca tctttttcta ttgctgtaaa catttctacc atttctgctt
    14281 cagctgcatc aaaggcatcc tgacgttctt gtttaccaaa aataacggct gcctcagcta
    14341 tttttagacc aaaggcattt tccaccctgt tcattatctc ttctggaatg ctatctataa
    14401 caacttccac cttttctttt ccgcaagcag aaataaaatc tttttgtaag gaacatagct
    14461 ttttaatttc attatggcca ataaagacag cttctaacat cgtttcttcg ctaagcacag
    14521 aagctgctga ttcaatcatc acaatagaat catcactacc agcaacggtt agattgagtt
    14581 tggattttga ttcaatctca tcaaaatggg ggtttactac aaactgatca tcaatgtaac
    14641 caactgtagc acctgcaata ggaccattaa aaggtatatc ggaaatcgat aacgctaagg
    14701 aagcaccaaa aatacccatc gtagaaggat ctgttactcc atcaaatgat agaacagtta
    14761 ctacgatatg aacttgattc cgaaagccct ctggaaatag aggacgaatt gaacgatcta
    14821 taacccttgc cattaaagtg gcatcagtag agggcttaga ttcgcgttta aaaaaaccgc
    14881 caggaatttt cccagctgca tacatctttt caatgtagtc tacggttaag ggaaagaaat
    14941 cctgattttc aacgggttct ttagacattg tagctgtaac taaaattgct gattctccat
    15001 attggataaa aacactgcca ttagcttgtt ttgccatttt accagtttct acaatcagtt
    15061 tgcgcccgca aaaatcggtt tctcgacttg taatattgaa gttatgcata acttctcctt
    15121 tttctatttt ggcgggagaa gaagcaatag gcgaaaaggt cttctttgta aaccttttgg
    15181 cttattgcta atttctcccg ctttatatta tttaaggttg acattttccc attcgataac
    15241 tgcttaaaat ataatattat cttctttcta ttttaactac agagcatttt tttagtcaac
    15301 ccattataaa gcgaaggaaa gtgaataata ggtaaattcc taaaattcac tttctcttgc
    15361 attgagcgat ccaaagttca gtattaatgc taaactatag atacactaag agtttatttc
    15421 cttatgccaa gcgccttgat taaatttcga tatctagtga tatctttctt ttttaaataa
    15481 tcaagtaaac gcctgcgttg cccgatcagt tttaacaagc cacgccgagt ggaaaaatcc
    15541 tttgggtgtt cttttagatg ctctgtaata tcttttattc tttgagttaa caaagcaact
    15601 tgaacttccg gcgagcctgt gtctgattca tggagtttaa actctgccat gatagcttgt
    15661 ttagcctcag gtgttaacgg catttcatcc tccagatgat tttatagcac catactttgg
    15721 taggtgacct atttgtccag taatattttt caatgttaat gagtttgctt ttttggtgga
    15781 ggtggcggga atcgaacccg cgtccagaaa accgatcacc cgaa
//
LOCUS       DAOSTH010000036        15133 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_4028_length_15133_cov_10.287439, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000036 DAOSTH010000000
VERSION     DAOSTH010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 15133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15133
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4028_length_15133_cov_10.287439"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            366..1922
                     /locus_tag="PLB65_05455"
     CDS             366..1922
                     /locus_tag="PLB65_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 5,6-aminomutase subunit alpha"
                     /protein_id="HOQ78009.1"
                     /translation="MLKLDLNQEMVNRAHNAASKITNAFTWLFDGYTTVAIERTVLRL
                     MGIDGALKDGKPLPNVVVDHLVENSAISKGAAIWVANAMLNKRLTAQEIAEEVAYKGL
                     DLSQIPLASGDAIFETLTPLTDKMLAQITSQKHKREELLSKYQPRPKPAIYVIVATGN
                     IYEDVVQARSAAREGADAIAVIRSTAQSLLDYVPFGATTVGFGGTFATQENFRIMRKA
                     LDEVSEEEKRYIHLTNYASGLCMPEIAAMGAMERLDMMLNDAMYGILFRDINPKRTLL
                     DQYFARMINAYAGIEIQTGEDNYLTTSDAVEKAYTVTASQLINESFAILSGVKPEKMG
                     LGHAYEIDPELENSFSYELAHALLTKTLFPNSPVKYMPPTKFASGNIFHTYLLNAMFN
                     FVGQLTEQGVQLLGMMTEAIHTPHLADRSLAIENAKYIFRAIKGLNQDLCLTEDSFIA
                     KRANEVLAQATEFLEKVAQEDLFSAMAKGEFAEIKRPENSGKGLEGVIMKDSSYYNPF
                     MESMKQELGL"
     gene            1919..2662
                     /locus_tag="PLB65_05460"
     CDS             1919..2662
                     /locus_tag="PLB65_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OAM dimerization domain-containing protein"
                     /protein_id="HOQ78010.1"
                     /translation="MSKNIIRPYGDTLNDGKMQLSFTLPVSCDALGKETARKLLLQMG
                     FDEAEITSMRDLTEGFTHFIAYAVTSTGVDISKIKVKVVETPVMTMEETDTYVAEHLG
                     RKITVVGACIESDAHTVGIDAIMNMKGYNHRYGLERYHSFNAINMGAQVTCEDLLAKA
                     REVNADAILVSQVVTQKNIHIKNLTRLIELAEAEGLRDKMLFICGGPRISFELAIELG
                     YDAGFGPGTYATDVASYIAITLAKQKAQE"
     gene            2688..3986
                     /gene="rseP"
                     /locus_tag="PLB65_05465"
     CDS             2688..3986
                     /gene="rseP"
                     /locus_tag="PLB65_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOQ78011.1"
                     /translation="MLTLLITIIAFGLMIFVHELGHFLVARSFRVNIEKFSIGFGKPI
                     WETEKNGIQYSLGWIPLGGYVKMKGENPEESIYLEEDDTFVGKSWWKRALIAFSGPFA
                     NLLFGILLFIIAFMLPQKQEDLLPVIQNAKGIWAEHFSPADSIYSINGKAVTGFQDFL
                     LKLSDKKNNTIEYIHNGQKATLEIAPTQIDSLIRSLEPKVDTTIGEVFTGMPAWKGGL
                     KPGDKILAVDSVAVSNWYEMRERIIGSKNEEVQLTVLRNGKILIRSIALEENVSMGNQ
                     KMIGITQYMPVKRVINYNPLEAISYGARSTLNFIVMNYVGLYKLIMKPEQLKNNVGGP
                     IMIATMGQQVAQRGFSSLIIFLASISLVLMIMNLLPIPILDGGHIFFAFFEGIFRKPV
                     PIKIQAFLQRIGFALLLLLMLYAFYADISKLLIRQLSLRQ"
     gene            3987..5096
                     /gene="tgt"
                     /locus_tag="PLB65_05470"
     CDS             3987..5096
                     /gene="tgt"
                     /locus_tag="PLB65_05470"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOQ78012.1"
                     /translation="MFNFTLQRTCGKARAGIIKTDHGEILTPVFMPVGTLGTVKAMSP
                     KELEEVQAQIILGNTYHLYMRPGHSLVQKAGGLHKFISWNHPILTDSGGFQVMSLAAL
                     RKISKEGVKFQSHIDGSAHIFTPEKVIEIQEALGADIIMSFDECPPYPSTRQYVEKSL
                     ATTLDWAERGKKAFTNCEHQALFGIVQGGIYEDLREQSALALIDMDFSGYGIGGLAVG
                     EEKEDMFRITSFLNDILPNDKPRYLMGVGTPSDLLNNIDRGVDMFDCVMPTRNARKGS
                     IFTRYGKMIIKAARYKDDFKPIDPLCGCYTCTHFSRAYIRHLITMNEILGMRLTTIHS
                     LWFYQELMQLARKAILENRWNEFLAEMLPVLDTIV"
     gene            5197..6867
                     /locus_tag="PLB65_05475"
     CDS             5197..6867
                     /locus_tag="PLB65_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78013.1"
                     /translation="MKTIVPELWTENWLYKRIYPLIFKVETEREQEQEFYTAFIQYSG
                     KTIANIFIVNLDVFDLLPDKPDKHIYNQILALGNSVHYKTRDWIIEKMLFFAEGDSFD
                     PQVMNRNLAYLKDLPYLREAELLISENENGTVDVFVLVRDKFSLELSGKIISHNKYRI
                     KINEQNILGLGLGLKHIWHIDPQALKSKSWESYYSNANIQGSFLRIDAFWKEFSGIST
                     QDLYLSHPFLYPAIPHSGGIELSRNYTHPPVDSVTTEQITLGSWYAHSFAYKDKEANA
                     YKYVAGSLEKNWFPKRPQVNDNSGKPWHENIFALNSIAFSKTGYTFLRDVSSYLENDV
                     IPVGYLWENLLGYEFGEYRNRLFAGTHYSRADKFGSGSYLYVSSALETYITTTGTEQS
                     ILAIEPLLISPLKQYGALRCRYFSNNNIIIGSKRYPKEKMNLSSLSGYRGNWNLQGNK
                     ILRSCIENDMAIPYTIWGFKIGLFTFLDFAFATDDLSQMSTKNVLLTEGTGFKMHNPS
                     LIWDSIELCFALNQKKGTKGEWVITLSTQKGIQLPDFSGKRPQTYKFY"
     gene            7106..11281
                     /locus_tag="PLB65_05480"
     CDS             7106..11281
                     /locus_tag="PLB65_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOQ78014.1"
                     /translation="MKTQVLKLLLLLFFLVNALSVFAVVSEYSFTSILGTFTEISGGT
                     IHGTSANDNECFIAIPLGFTFTYNDVDYTTISIASNGFIAMGDTVATSTVPISSATST
                     NNVIAVLSRDIKSRAEGTLMSLSSGTAPNRVFTIQWKNWRRSPTATANDDFTFQIQLQ
                     ENGNKVVFVYGPFTAVTASTANTVQVGLRGDSNADFNNRTTTTDWSATIAGTANNNSC
                     TLSASVFPANGLTFTFTPPVTGEPPLAAQNPHPANGAINVPIGTNLTWITGGGIVDGY
                     KVYFGTDNPPTNIVSGVIQTATVYDPANDLNYSTTYYWKIVPFNQFGDAVDCPVWSFT
                     VLADPTVTTYPYEQNFDLVTVPDLPPGWTVINANNDTYTWQSYNANSQTPPNSMRIRY
                     NAAIAMDDWLISPPLVFTDTHNYKIKFYYCGGGSNYEEKLSVYWGTTPTAAGLTNLLW
                     QNLNITNTTYETAEIILPSVSGGTYYIGFHGHSDADKFYIYLDTFSVMDIVEQLDPPQ
                     NLTATVQDNYNVHLDWDAPGAVPPPPTNFTDGFETYTDFSLEFTPWTLVDVDQSSTYG
                     ISGFSWLNIYAAQAYIIFNPSATTPAVTTLTAHTGNKMAACFAATTPPNNDWMITPLL
                     NITAGTVLKFWARSYTAQYGLERFKVGVSTSGTAPANFNIISGTNYIQAPVEWTEYTY
                     DLSSYVGLSIYIGIQCVSSDAFIFLVDDVFIGNPTDAKVYPYVASVPGTIERVVGIPA
                     PTIKQEIKHIDRDLLGYKVYRDGNLIDTINNVSTTDYTDLALAVGTYSYTVTAFYTTG
                     ESVPAGPVSVTIVAPLLPPTNLTATVDGNDVTLNWGNPEPPPPGQWITWCNDVLGNSI
                     GTNSAANFDVAHRWPVADLAPYVGDAITQVKFVPAEANCVYTVKIWTGGSANNAGTLV
                     YSHQIQNPVIDSWNVHALTTPVTIPANSEVWIGYNCNTMVGFPAGCDIGPQIDGKGNM
                     MYFQGVWSTLLAIEPALTYNWLISTYVSQGATLKKMEMKPIADLHPPVKNSGELGLLH
                     KEIANLQDRVVTGYKVYRNGALLTTISDPTNTTYSDLDLANGTYTYGVSALYPSGESV
                     PATVEAIVNIQLAPAFFTDDFESYPDFATMFAPWTSLDVDLSSTYVITGIEFPGSGSP
                     MAFIVFNPSSTVPPATTIIPHSGTKMVASIAATTPPNNDWLITPRVYLGTNAALKFYA
                     KSHTAQYGMERFRIGISTQSHINPQGFQYITGNDYVEVPTNWTEYIYDLSVYDNQNVY
                     IGIRCVSNDAFIFFADDISIHGEGGHIVSNDDELAPVVRTELKGNYPNPFNPETTIAY
                     SVKETLPVTIEIYNLKGQKVTTLVNETLVPGNYSIVWKGLDDNNHPVASGVYFFKMNA
                     GKYSSTKKMIMMK"
     gene            complement(11505..>15133)
                     /locus_tag="PLB65_05485"
     CDS             complement(11505..>15133)
                     /locus_tag="PLB65_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOQ78015.1"
                     /translation="GMTNAIISNTNITNGEYQRLEGIFTPTASGLYYIGILGTLNYSP
                     YYLSIDDITIELAPSVIEPSNLIVSSITGNSAVLGWTENNIPPATTWDIIYGAPGFNP
                     NGKATQIQVTSNPYTLTGLQPSTTYEWYVRAVKDGNTSVWAGPSSFTTAQIPAELPLT
                     ENFEGDINWTIVNGTQTNKWFVGSVPAYSGNSAYISKDNGTTYEYNNGSSSVVHLYRD
                     ISFPAGALEYNLSFMFKCGGESTYDGLKVFLVDTATVPTAGSQLSTEQIGNTWYNLTT
                     AWTEKTITINNAVAGTTKRLVFSWINDGSGGTQPPAAIDNIELTYTMPNPSPEPPSNP
                     VPSNVATNVPLNQILSWTNNGTVTKVDVYFGLTDNMVLVSENQTDPLNSYDPELEYSK
                     TYQWKIVAKNDAGETAEGPIWTFSTMVDPTRSIPYLETFDASTSLPANWTTDYFSVGS
                     SHGVSSNGLYKNLYDYSTSAYVTTPPVGPLTDNTTLQFDYRYVEYSGYPSTAHTLVAG
                     DKLEIQISTDGTNFTTIHTIDSSNHITSTSFATCSVPITQAKVSQDDIIKIKFLATRG
                     EGDYYLDIDNVYFRHISAEPIFSVTPESKAFGEFQINTVSSAQVFTIKNNGGGTLTIN
                     PALELIGANADQFQLTDTNSYPCELGAGATMTVSVAFAPTSIGEKTANLKIVDNLSKT
                     EHNIPLTGTGVDYTIYTIPYLQGFDAVIFPPAGWKNEKISGTGSGLWDRVTSGSSPSC
                     ATHSGAGMARFNAFSMSNGTHGILATPPVQLESGQYYIDFWMYRDSGYSTYQDEGIKV
                     YINATPSLTGATEIAYIHRPINLEPVVASSGWYEYNYVLDNLAGTKYFIFEGISNYGN
                     NIFLDDIRILKPDTQINQGTASGGYVSIIIPSVHTISPTIEIANLGQSDATVTVIAGY
                     ADVALPNAGLSLTLTGASFSGTTITINHNLGFSPSQIAYQILPNGWNILTPTNPSVDI
                     WNNTTVSFTISDKSDGDVAIVFPQEAGQTLPVTLSNFTAVVTSENFVNIAWTAETETN
                     HSGYNLYRNEAKDLDNAIKINAHLIDQGSEIGTQNNYFYTDFEVYTNIVYYYWLESVS
                     LNGASDFYGPLTVTIGDPTQEPLPPVVPMVTKLYNAFPNPFNPNTNIRYSLKEAGKVN
                     IEIYNMKGQRIKAYHQEHNAPGYYQVSWDGRDENGRVVSSGIYLYRLTTGKYTAAKKM
                     ILAK"
BASE COUNT         4725 a         2704 c         3015 g         4689 t
ORIGIN      
        1 ttagccaccg aggacaccga aatcaccgaa aaaggacctg gaactttaaa cttttgaact
       61 tagaaacttg acaagtgagg tcccctaatc tttttcatct catcaatatt gaagctcaaa
      121 aagttgtact atgatccatt cattcctgtt tactcctgag tagttgacct attatcatcc
      181 gctcctcatc tttccctccc ctcctctttg aggaagagtt aaggaggaga ggaagatgag
      241 tggatgagga gttaaggagg agaggtaata aaggatcaag caagagaaaa acaaaatccc
      301 ttgactttat aggcagttcc aaaattatag aaaaaacttc acttttcaat atactggaga
      361 gataaatgct aaaattagac cttaatcagg aaatggtaaa cagagcacat aatgctgcat
      421 ctaagattac aaatgcattt acctggcttt ttgatggcta cacaaccgta gcaatagaaa
      481 ggacagtttt gcgactgatg ggaattgatg gtgcacttaa agacggtaaa cctttaccta
      541 atgtggtagt agatcactta gtggaaaatt ctgctatttc aaaaggagca gcgatatggg
      601 tggctaatgc aatgttaaac aagaggctta cagcacaaga aatagccgaa gaagttgcct
      661 ataagggttt agacctcagt caaattcctc ttgcctctgg cgacgcaata tttgaaactc
      721 ttactccttt aaccgataag atgttggctc aaataaccag ccaaaaacat aagcgtgaag
      781 agcttttgtc caaatatcag ccccgtccca aaccagcaat atatgtaatt gtagctacgg
      841 gaaacatata tgaagatgtg gttcaagctc gttcagcagc aagagaaggt gcagatgcga
      901 ttgcagttat tcgttcaacg gctcagtcgc tattggatta tgtgcctttt ggagctacga
      961 cagtgggttt tggtggcact tttgccaccc aggaaaattt caggattatg cgcaaagctc
     1021 tggatgaggt ttcggaagaa gaaaaacggt atattcactt aactaattat gccagtggac
     1081 tttgtatgcc tgaaattgca gctatggggg caatggaacg attggatatg atgttaaatg
     1141 atgctatgta tggtattctt tttcgggata ttaatcctaa gcgaaccttg ctggatcaat
     1201 attttgcgcg aatgataaat gcctatgcag gaattgaaat tcagaccggt gaagataatt
     1261 atttaactac ttccgatgct gtagaaaaag catatacggt tactgcaagt cagttgataa
     1321 atgagagttt tgctatttta agtggtgtta aaccggaaaa aatgggtttg gggcatgctt
     1381 acgagataga tcctgagctt gaaaacagct ttagttatga attggcacat gcccttttaa
     1441 caaaaaccct ctttcctaat tcaccggtta aatatatgcc tccaacaaag tttgccagtg
     1501 gcaatatatt tcatacctat ttgctgaatg cgatgttcaa ttttgttggt cagttaacag
     1561 aacagggagt acagctttta ggaatgatga cggaggcaat tcatactcct catttagcag
     1621 atcgctcttt agctatcgaa aatgcaaaat atatatttag agcgattaag ggattaaatc
     1681 aagatttatg tttgacagaa gatagtttta tcgccaaaag agctaatgaa gttttggcac
     1741 aggcaacaga atttttggaa aaagttgccc aagaagatct ttttagtgct atggctaagg
     1801 gcgaatttgc agaaattaag cgtcccgaaa acagcggtaa aggtctggaa ggtgtaatta
     1861 tgaaagatag ttcatattac aatcctttta tggaaagtat gaaacaggag cttggattat
     1921 gagcaagaat ataattcgtc cctatggaga taccttaaat gatggcaaaa tgcagctttc
     1981 ttttacgctg ccagtttcgt gtgatgcttt aggaaaagaa acagccagaa aactactatt
     2041 acaaatgggt tttgatgaag cagaaattac tagtatgaga gatttaactg agggtttcac
     2101 tcattttatt gcttatgctg ttacatccac aggagtagat atcagcaaaa taaaagtaaa
     2161 agtagttgaa actccggtta tgactatgga agaaaccgat acctatgtag cagaacactt
     2221 aggaagaaaa attacagtag taggcgcctg tattgaaagt gacgcacata cagttggaat
     2281 agatgctata atgaatatga agggctataa tcatcgttat ggtttagaac gctatcacag
     2341 ttttaatgct ataaatatgg gtgcacaagt tacctgtgaa gaccttttag ccaaagccag
     2401 agaagtgaat gcagatgcaa ttttagtttc gcaggtagtt acgcaaaaga atattcacat
     2461 caaaaactta actcgcttga tcgaacttgc agaagctgag ggtctgcgtg ataagatgct
     2521 gtttatttgt ggtggaccgc gtatttcttt tgagcttgct attgaacttg gttatgatgc
     2581 aggttttggg cctggaactt atgctacaga tgttgcaagt tatatagcta ttacattggc
     2641 aaaacaaaaa gctcaggaat aggcaatcag gatttaagcg taacattatg ctaacattat
     2701 taatcacgat aattgccttc gggctaatga tctttgtgca tgaattaggt cactttttgg
     2761 tagctcgctc atttcgggtg aacattgaaa aattctctat aggttttggg aagccgattt
     2821 gggaaactga aaaaaacggc attcaataca gtttaggatg gattcctttg ggtggttatg
     2881 taaaaatgaa aggagaaaat ccagaagaga gtatctactt agaagaagat gacacttttg
     2941 ttgggaaatc ttggtggaaa agagctctca tagctttcag cggaccgttt gctaatttgc
     3001 tttttgggat cttacttttt attattgcct ttatgttgcc gcaaaaacaa gaagatcttt
     3061 tgccagtaat tcaaaatgct aaaggaattt gggcagaaca tttcagtcct gcagatagca
     3121 tatatagcat taatggtaaa gctgttacag gttttcaaga ttttctactt aagctatccg
     3181 ataaaaaaaa taatactata gagtatattc acaatggaca aaaagccact ttggagattg
     3241 ctcctacaca aatagattct ttaattaggt ctcttgagcc aaaagtggat actacaattg
     3301 gagaagtttt tactggaatg cctgcttgga aaggaggttt aaaaccaggt gataaaattt
     3361 tggctgtaga tagtgtagca gtttccaatt ggtatgaaat gcgcgaacgg ataattggct
     3421 ctaaaaatga ggaagttcaa cttactgttt tacgcaatgg aaagattttg atccgcagta
     3481 ttgctttgga ggaaaatgtc tctatgggaa atcaaaaaat gattggtatc acgcaatata
     3541 tgccggtgaa aagagttatc aattataatc ctttggaagc aattagctat ggcgcaagga
     3601 gcacattgaa ttttatagtg atgaattatg tgggcttata caaactgatt atgaaaccag
     3661 agcagttaaa aaacaatgtt gggggaccta taatgattgc tacaatgggt caacaagttg
     3721 ctcaacgagg ttttagttcc ctaatcatct ttttggcttc cattagttta gttttgatga
     3781 taatgaatct attgccaata ccgattcttg atggtggaca tatattcttt gccttttttg
     3841 aaggtatttt cagaaagcct gtaccaatta aaattcaggc atttttacag agaattggtt
     3901 ttgccctact gctattattg atgctatatg ctttctatgc agatatatcc aagttgctaa
     3961 tccgtcagtt atcattgagg caatagatgt tcaattttac tcttcaaaga acttgtggaa
     4021 aagcaagagc aggaataata aaaacagatc acggtgaaat actgacccca gtttttatgc
     4081 cggtgggcac actgggaaca gtgaaagcaa tgagccccaa agaattagaa gaagttcagg
     4141 cacagataat cttaggcaat acctatcatt tgtatatgcg ccccggacat tcattagtgc
     4201 aaaaagcagg tggtttacat aaatttatca gctggaatca tcctatttta acagatagcg
     4261 gtggttttca agttatgagt ttagctgctt tacgcaaaat cagtaaagaa ggcgtaaaat
     4321 tccaatccca tattgatggt tctgctcata tattcacccc ggaaaaagtg atagaaattc
     4381 aagaagctct tggtgcagat ataattatga gctttgatga atgccctcct tatccttcta
     4441 ccaggcaata tgtagaaaaa tcgctggcta ctactttaga ttgggcagaa agaggcaaaa
     4501 aagcatttac caattgtgaa catcaggcac tttttggaat agttcaaggt ggaatttatg
     4561 aggatctaag ggaacaatct gctttagctt taatagatat ggatttctcg ggttatggaa
     4621 taggcggtct tgctgtggga gaagaaaaag aagatatgtt taggattact tcctttttaa
     4681 atgatatttt gcctaatgat aaacctcgct atttaatggg tgttggaact cccagtgatt
     4741 tattgaataa tatagaccgt ggagttgata tgtttgattg tgtgatgcca actcgtaatg
     4801 cccgcaaggg aagtattttt acccgttatg ggaaaatgat tattaaggcg gcacgctata
     4861 aagatgattt taagcccatt gatcctttat gcggttgtta tacttgcact catttcagcc
     4921 gtgcatatat aagacattta attactatga acgaaatttt gggaatgcgg cttaccacaa
     4981 ttcacagttt atggttctat caggaactaa tgcaactggc aagaaaggct attctggaaa
     5041 acagatggaa tgaatttctg gcagaaatgc tccccgtgct cgatacgatc gtttagtaaa
     5101 aaagctgtaa tcaatttatt ctgctttctt accctaatgc tcttctgcag tataatctac
     5161 tgccaggaag ctcaaaatgt tacaattact gattccttga aaacaatagt ccccgagctc
     5221 tggacagaaa actggcttta taaaaggatt tatcctctta tttttaaagt ggaaacagaa
     5281 cgcgaacagg aacaagaatt ttatacagct tttatacaat atagcggtaa gacaattgct
     5341 aatattttca tagttaatct tgatgtgttt gatctgcttc ccgataaacc tgataagcac
     5401 atatacaatc aaatcttggc tttgggaaat tccgttcatt ataaaacgcg ggattggata
     5461 atagagaaga tgcttttttt tgctgaaggc gatagcttcg atccacaagt aatgaaccgt
     5521 aatcttgcat atttgaagga tttaccatat ctgagagaag ccgaactgct gatcagtgaa
     5581 aatgaaaatg ggacagttga tgtctttgtg ttggtgagag ataaattttc cttggaacta
     5641 agcggtaaaa ttatctccca taacaaatac cggattaaga ttaatgaaca aaacatcctt
     5701 ggcttgggtt tagggctaaa acatatctgg catatagatc cacaagctct gaaatccaag
     5761 agctgggaaa gctattatag caatgccaat attcaaggta gcttcttgcg tattgatgct
     5821 ttttggaaag aattttcagg aatatcaacc caggacttgt atttaagcca tccttttttg
     5881 tatcctgcta tcccccattc aggaggaatt gaactttcca gaaattatac ccatccaccc
     5941 gtagattctg ttactacaga acaaattact cttggttctt ggtatgcaca tagttttgcc
     6001 tacaaagata aagaggcaaa tgcttacaaa tatgtggccg gttctttaga aaaaaactgg
     6061 tttcccaaaa gaccacaagt aaatgataat agcggtaaac cttggcacga aaacattttt
     6121 gccctaaatt cgatagcatt cagtaaaacg ggttacactt ttttacggga tgtgagctcc
     6181 tatctggaga atgatgttat acctgttggt tatttatggg aaaatctctt gggttatgaa
     6241 tttggagaat atcgcaatcg actttttgct ggcacacatt attctcgagc agataaattt
     6301 ggtagcggtt catatcttta tgtatcgagt gcactcgaaa catatattac tacaacaggt
     6361 accgaacaaa gcattttggc tattgaacca ttacttattt caccacttaa acaatatgga
     6421 gctctccgct gtagatattt cagcaacaat aatattataa tcggcagtaa aagatatcct
     6481 aaagaaaaaa tgaacctttc gtccctatca ggatataggg gaaactggaa cttacaaggg
     6541 aacaaaatct tgcgctcctg tatagaaaat gatatggcta ttccctatac aatttggggg
     6601 tttaagatag gattatttac ctttctggat tttgcctttg ccacagatga cctatcccaa
     6661 atgagcacaa aaaatgtgtt gctgactgaa ggcacaggtt ttaaaatgca taacccttcg
     6721 ctgatatggg attccattga actctgcttt gccctaaacc aaaaaaaagg tacaaaagga
     6781 gaatgggtaa taactctctc tactcaaaaa ggaattcaac ttcccgattt ttcagggaaa
     6841 agacctcaga cctacaaatt ctattaaaaa ggaagagtgc gtatttcata aagctttaac
     6901 tgtagggcta tgcttttaat ataaataaaa acacatcctt ttttgtgcta cgcattaatt
     6961 ccctttttcc caataagttt ttgcttgaca ttatattata agctattata atgtacaata
     7021 gttgcatttt ggataatgga tttatgtaaa catccaaaat attctgtaga ataagaaagt
     7081 gaattactaa aagaaaggag atactatgaa gactcaagtt ttgaaactcc tccttctgct
     7141 gtttttttta gtgaatgctc taagtgtgtt cgccgtagtt agcgaatatt cttttacctc
     7201 tatattgggg acatttactg aaatcagtgg ggggactatt cacggtacca gtgcaaatga
     7261 caatgaatgt tttatagcca ttccactggg ctttactttt acctataacg atgttgatta
     7321 tactactatc agcatcgctt ccaatggttt tattgctatg ggtgatactg tagcaacaag
     7381 tactgttccg atcagttctg caacttctac aaacaatgtt attgcggttc tttcccgcga
     7441 tatcaaatcc agagcagaag gaactctaat gtccctaagt agtgggactg ctcccaatcg
     7501 ggtttttacg attcaatgga aaaattggcg gagaagtcct actgcaactg caaatgatga
     7561 ttttactttt caaattcaac ttcaagaaaa tggcaataag gtggtctttg tttacggacc
     7621 gtttactgct gtaactgctt caactgcaaa cacagtgcaa gtgggcttaa gaggtgattc
     7681 caatgctgat tttaataatc ggacaactac aactgattgg tctgctacta tagctggaac
     7741 ggctaataac aattcctgta ctcttagtgc ttctgttttt cctgctaatg gtttaacttt
     7801 taccttcaca cctcctgtaa cgggtgaacc acctttagca gctcaaaatc ctcatcctgc
     7861 caatggtgct ataaatgttc ctattggtac aaatcttact tggattacgg gtggaggcat
     7921 tgtagacggt tacaaggttt attttggaac cgataatcct cctactaata tagtgagtgg
     7981 tgttatccaa actgcaacgg tgtatgatcc tgccaacgac ctaaattaca gcacaaccta
     8041 ttattggaaa attgtaccct tcaaccagtt tggagatgca gttgattgtc ctgtatggag
     8101 ctttacagtt ttggcagatc caactgttac tacctatcct tatgaacaga attttgatct
     8161 ggtaactgtt ccagatctac ctcctggatg gacagttatc aatgccaata atgatactta
     8221 tacttggcaa tcatataacg ctaatagtca aactccacct aattcaatgc gcattcgtta
     8281 caatgcagca atagctatgg atgattggtt gatttcgccc ccgcttgttt tcactgatac
     8341 tcacaattat aagataaaat tctattattg tggaggcgga tcaaactatg aggaaaaatt
     8401 gtctgtctat tggggaacta cacctacagc tgcaggttta acaaatttac tatggcagaa
     8461 tttgaatatt accaatacta cctatgaaac agctgaaata attctacctt ctgtttcagg
     8521 gggaacctat tacattggtt ttcacggtca tagtgatgct gacaaattct acatttattt
     8581 ggatactttc agcgtaatgg acattgtaga acagttagat cctccccaga atttaactgc
     8641 tacagtacag gataactata atgtacattt ggattgggat gctcctggtg cagttcctcc
     8701 acctcctaca aattttactg atggttttga aacctatact gatttttctc ttgaattcac
     8761 cccatggact ttagtagatg ttgaccaaag ctcaacttat ggaattagcg gcttttcttg
     8821 gctaaatata tatgcagccc aagcatatat tatttttaat ccaagtgcta caactcctgc
     8881 tgtaaccact ctgacagctc ataccggaaa taaaatggct gcctgttttg ctgcaacaac
     8941 ccctcctaat aatgattgga tgattactcc cctactaaat attacagcag gcactgtctt
     9001 aaaattctgg gcaagaagtt atactgctca atatggcttg gaacgtttta aggttggggt
     9061 ttctacatca ggaacagctc cagcaaactt caatattatt agtggaacaa attacattca
     9121 ggcaccagtt gaatggacag aatatactta tgatttaagc agttatgtgg gactaagtat
     9181 ttacattggt attcaatgtg tttctagtga tgctttcatc tttctggtag atgatgtgtt
     9241 tatcggtaat cctactgatg caaaagtgta tccttatgtt gcatctgttc ctggaactat
     9301 agaaagagta gtaggtattc ctgctcccac tattaaacaa gaaatcaaac atatcgatcg
     9361 tgaccttttg ggctataagg tatatcgtga tggtaacttg attgatacta ttaataatgt
     9421 ttccactact gattatactg atctagcttt ggctgtggga acttatagct atactgttac
     9481 agcattttat acaactgggg aatcagtccc tgcgggtcct gtaagtgtaa ctattgtagc
     9541 tcctttgtta ccacctacaa atttaacagc gaccgtggat ggtaacgatg ttactttgaa
     9601 ttggggaaat cctgaaccac ctcctcccgg acagtggatt acttggtgta acgatgtttt
     9661 gggaaacagc atcggaacta attctgcagc taattttgat gtggcacatc gttggcctgt
     9721 tgcggatctt gctccttatg taggagatgc tatcactcag gttaaatttg ttcctgctga
     9781 agctaattgt gtttatacag tgaaaatctg gacgggcgga agtgctaata atgctggaac
     9841 tttggtttat tctcaccaaa ttcaaaatcc tgttatagac agctggaatg tccatgcgct
     9901 aactacaccg gtaacgattc cagctaacag cgaagtatgg attggctata attgcaatac
     9961 aatggtggga tttcctgcag gttgtgacat tggtcctcaa attgatggta aaggcaatat
    10021 gatgtatttt cagggtgttt ggagtactct tcttgctatt gaacctgctc ttacctataa
    10081 ctggttaatt agtacttatg taagccaggg agccactttg aaaaaaatgg aaatgaaacc
    10141 tattgctgat cttcatcctc ctgtgaaaaa cagtggtgaa cttggtctgt tgcataaaga
    10201 aatagctaat cttcaagata gggttgttac cggctataag gtttatcgta atggagcctt
    10261 attaactact atctctgatc ctaccaatac tacttattct gatcttgact tagctaacgg
    10321 aacttacact tatggcgtaa gtgctttgta tccaagtgga gaatctgttc ctgcaaccgt
    10381 tgaagcaatt gtaaatattc agcttgctcc tgctttcttt acagatgatt ttgaaagcta
    10441 tcctgatttt gctacaatgt ttgccccgtg gacttcattg gatgtggatc tatcttcaac
    10501 ttatgttatt acagggatag aatttccggg tagcggaagt cctatggctt tcattgtttt
    10561 caatccctct tcaactgttc cccctgctac tactattatt cctcatagtg gaacaaaaat
    10621 ggttgccagc attgcagcta caactcctcc taataatgat tggttgatta ctcctcgtgt
    10681 ttatctgggt acaaatgctg ctctaaaatt ctatgctaag agccatactg cccaatatgg
    10741 aatggaacgt tttcgtatcg gaatatctac tcagtctcat ataaatcctc aaggtttcca
    10801 atatataact ggaaatgatt atgtggaagt tcccactaac tggacagagt atatttacga
    10861 tctttctgtt tatgataacc aaaatgtata tatcggaatt cgctgcgttt ctaatgatgc
    10921 tttcatcttt tttgcagatg atataagcat tcatggcgaa ggcggtcata ttgttagtaa
    10981 cgatgatgaa ctggctcctg ttgtccggac agaattgaag ggaaattacc ccaatccttt
    11041 caatccggaa acaactatcg cctatagcgt gaaggaaaca ctaccggtaa ctatagagat
    11101 atataacctt aaaggacaga aagtaactac tctggttaac gaaactttag tgccaggtaa
    11161 ttattctatt gtctggaaag gactggatga caataatcat cctgtagcca gtggagtata
    11221 tttctttaag atgaatgctg gaaagtatag cagcactaag aaaatgataa tgatgaagta
    11281 attcgttaaa acaaatgata atcaaccccg agccaattta gctcggggtt tctttttctc
    11341 caggaagatt gatgtcctca tcaacccaca ataaacatac tatcaagcta ttatagaatt
    11401 tagcggaaac tacataatct ggtgtaagga aaacgacagg atgtcgttct tcaaaaaaaa
    11461 gcggagcctt ggaaagctcc gctactgaat aagttgttaa ccttttattt tgctaatatc
    11521 atctttttgg ctgctgtgta tttacctgtg gttaaacgat acagataaat tccgctggaa
    11581 acaactctgc cattttcatc acgaccatcc caagaaactt gatagtatcc aggagcatta
    11641 tgttcttgat gataagcctt aattctctgt cctttcatat tgtagatttc tatatttacc
    11701 ttacctgctt ctttcaggga ataacgaata ttagtgttcg gattgaaagg attggggaaa
    11761 gcattataga gttttgtaac cattggaaca acaggaggta atggttcttg agtaggatct
    11821 ccaatagtaa ccgttaaagg accatagaaa tcactggctc catttagtga aacactttcc
    11881 agccagtaat aatacactat attggtatag acctcaaaat cagtatagaa ataattgttc
    11941 tgagttccaa tttctgatcc ctggtcaatt agatgagcat taatcttaat cgcattatcc
    12001 aagtcctttg cttcattcct gtaaagatta taaccggagt gattcgtttc agtttctgct
    12061 gtccaggcaa tattaacaaa attctctgaa gttactacag cagtgaaatt agatagagtt
    12121 acgggtaaag tctgacctgc ttcttgaggg aatactatag caacatcacc atctgattta
    12181 tccgagattg tgaaactaac ggttgtatta ttccaaatat ctacagaagg atttgtagga
    12241 gtaagtatat tccaaccatt aggtagtatc tgataggcaa tctgagaagg gctgaaacca
    12301 agattatggt taatagtgat tgttgtgccg gaaaaactgg ctccagtaag tgttaagcta
    12361 agacctgcat tgggaagagc aacatcagca tagccagcaa taacagttac ggttgcatca
    12421 ctttgtccaa gattggcaat ttctatcgtg ggagaaatag tatgaacaga agggataatt
    12481 atactaacat atcctccgga agcggttcct tggttaattt gagtatccgg ttttaatata
    12541 cggatatcat ctaagaaaat gttattacca taattactga ttccttcaaa gatgaagtat
    12601 tttgttccgg ctagattatc taagacataa ttatattcat accagccaga agatgcaact
    12661 accggttcca agttaattgg tctatggata taagcaattt ccgtagctcc ggttaacgaa
    12721 ggggtggcat tgatatatac ttttatccct tcatcttgat aagtactata accggaatcc
    12781 ctatacatcc agaaatcaat gtaatattgt ccactttcta attgaactgg aggtgtagct
    12841 aaaataccgt gagtaccatt agacatacta aaagcattaa aacgagccat tcctgctcca
    12901 ctgtgagttg cgcaagaagg gcttgaacca gaggtaacac gatcccaaag cccacttcct
    12961 gttccggaaa ttttctcatt tttccagccc gcaggaggaa agattacagc atcaaatcct
    13021 tgcaaatatg gtatagtata tatagtgtag tcaaccccgg ttccggttaa aggaatgtta
    13081 tgctctgtct tacttaaatt atcaactatt tttagatttg ctgtcttttc acctatagaa
    13141 gtaggagcaa aggcaacaga aacagtcatt gttgcacccg ctccaagttc acaaggatag
    13201 ctattagtat cagtaagctg gaattgatct gcattagcac caattagttc aagagcagga
    13261 ttaatggtta atgtcccacc gccattgttt ttaatagtga agacctgagc agaagaaact
    13321 gtattaattt gaaattctcc aaacgccttg ctttcaggag ttacagaaaa gataggttca
    13381 gctgagatat gtctgaaata tacattatca atatccagat agtaatcacc ctcacccctg
    13441 gttgccagga atttaatctt gataatatca tcttgagaga cctttgcttg ggtaatagga
    13501 acactgcagg tagcaaagga agtggaagta atatgattac tgctatcaat tgtgtggata
    13561 gttgtgaaat ttgttccatc agtagaaatc tgaatttcca gtttatcacc agctaccagt
    13621 gtatgagcag tgctagggta tccggaatat tcaacatagc gataatcaaa ttgcaatgta
    13681 gtattatcgg ttaaagggcc tacaggaggt gtagttacat aagccgaagt tgaatagtcg
    13741 tataagttct tatacaatcc attgctacta acaccatgac ttgaacctac tgagaaataa
    13801 tctgtagtcc agtttgcagg taaagaagta ctggcatcaa atgtttcaag gtaaggaata
    13861 gatcttgtag gatcaaccat tgtagaaaaa gtccagatag gaccttctgc tgtttcacct
    13921 gcatcatttt tagccacaat tttccattga tatgttttgc tatattcaag ctcaggatca
    13981 tagctattca agggatctgt ttgattttca gaaaccaata ccatattatc agttagccca
    14041 aaatagacat caacttttgt tactgtgcca ttatttgtcc aggaaaggat ttgatttaat
    14101 ggaacattag tagccacatt agaaggaaca ggattacttg gtggttcagg agaaggatta
    14161 ggcattgtgt aagtaagttc aatattatct atagcggcag ggggttgtgt ccctcctgaa
    14221 ccatcattta tccaggaaaa gaccaggcgt tttgtagtac ctgctacagc attgttgata
    14281 gtgattgttt tttccgtcca agcagtagtt aggttatacc aagtattgcc tatttgttct
    14341 gtagaaagtt gtgaccctgc tgtaggaaca gtagctgtat caactaaaaa tacttttaat
    14401 ccatcataag tagattcacc gccacactta aacataaagc ttaaattata ttccagagca
    14461 ccagcaggga aagaaatatc acggtataaa tgaactactg aacttgaacc attattatac
    14521 tcataagtgg ttccattgtc tttagaaatg taagcagagt ttcctgaata agcaggaaca
    14581 gagcccacaa accatttatt agtttgagtt ccatttacaa tagtccaatt tatatcacct
    14641 tcaaagtttt cagtaagtgg taattcagct ggaatttgag cagttgtaaa actggaagga
    14701 cctgcccaaa cacttgtatt gccatcttta acagcacgca cataccattc ataagtagta
    14761 cttggctgaa gacctgtaag ggtataggga ttggaagtaa cttgaatttg agttgctttt
    14821 ccattaggat taaaaccagg tgccccataa ataatatccc aagtagttgc tggaggaata
    14881 ttattttctg tccaacctaa aacagcagag ttaccagtaa tactactcac aataagattg
    14941 ctgggttcaa taaccgatgg agcaagttca atcgtaatat catcaataga aagataataa
    15001 ggtgaataat taagagtccc taatatacct atatagtata aaccacttgc agtaggagta
    15061 aatatacctt ctaaacgctg atattcacca tttgtaatat ttgtattgga tataattgca
    15121 ttagtcattc ctg
//
LOCUS       DAOSTH010000037        14986 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_4074_length_14986_cov_10.219945, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000037 DAOSTH010000000
VERSION     DAOSTH010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14986
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4074_length_14986_cov_10.219945"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..311
                     /locus_tag="PLB65_05490"
     CDS             78..311
                     /locus_tag="PLB65_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78016.1"
                     /translation="MTITNDGNNTPTDMIVDETTGRLLVKIVVEDITTEPRSIDIDAN
                     QEYVGLVLADDDTTIVPLMVNKDGAIYANLVVE"
     gene            346..621
                     /locus_tag="PLB65_05495"
     CDS             346..621
                     /locus_tag="PLB65_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78017.1"
                     /translation="MTDITYPIDDNHNPITGLDMIDSKSITYVAATTGAVGVTNLFTI
                     IGTVALRIFGVCSVNLTGSGTLEVGIAGMTNGLIDQTTGTDIDKDTI"
     gene            807..1283
                     /locus_tag="PLB65_05500"
     CDS             807..1283
                     /locus_tag="PLB65_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78018.1"
                     /translation="MADESAKIDVNQQKTLLAVTNAATPEIKNVRCDATTGRVLVSAT
                     VSGVSDITVANEATDTSCFPLFATAATGSIQPKTNAGLTFNSNTGNLGATLMNTHKIT
                     VGVAGTATGSIELNGTTSGTVTLKTADAAGTYTLTLPTNDGNANQFLQTNGNGVLT"
     gene            1416..1871
                     /locus_tag="PLB65_05505"
     CDS             1416..1871
                     /locus_tag="PLB65_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78019.1"
                     /translation="MFCETIELGHDSDTTLSRSAAGVLAVEGVVIPTISSADTLTNKR
                     ITPRVYSEASNATPTPNIDSYDMYELTALAVSATFSAPTGTPTDGQSLIIRIKDNNNA
                     QSLSWNAIYRASTDLALPTTTSANKTMYLGFKYNSADSKWDLLAYLNNI"
     gene            1882..2355
                     /locus_tag="PLB65_05510"
     CDS             1882..2355
                     /locus_tag="PLB65_05510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78020.1"
                     /translation="MAIFYLDYENGNDAARSSVNITSSDNNGSGLVRILTAANHGLVT
                     GAKVTIAGHSVSEYNGDWVITNIDATHFDLQNSTYSSSGTGGTETPFGGMNWADAWKT
                     ITNGATAARIAPGDIIRIAKSPAPVSIGSASWTNLSKTVVLENAQTLNVDMCENV"
     gene            2659..3129
                     /locus_tag="PLB65_05515"
     CDS             2659..3129
                     /locus_tag="PLB65_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78021.1"
                     /translation="MTLAREGSGNLGNAIKSIAVYTDSTAPTASSYIFIDDFIACTTN
                     GLNLQSLISKNSNEQGGTEGWYCIKNINGTTILLDNDTNSKANAGRGYYGTTETVTTY
                     KRETIKTAMAGSSSTAIQEVKDSGTSGSKIEFQGGFNTSNNNQEGETFFDGLNG"
     gene            3139..3627
                     /locus_tag="PLB65_05520"
     CDS             3139..3627
                     /locus_tag="PLB65_05520"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HOQ78022.1"
                     /translation="MYLNGKSYTILNYLNVCRYYNGIYYYNISNNNTITTLSNANNNS
                     NYGIYYNSSNNNTITTLGNVNNNSSHGVSYAQSNNNTITTLGNVNNNSGYGVYYDQSS
                     NNTIKSLSTSGNSTGGVYNNTGVNYLFNAKIQEATEVDGFQNFANSRIFSQNHDQIAD
                     NH"
     gene            3751..3912
                     /locus_tag="PLB65_05525"
     CDS             3751..3912
                     /locus_tag="PLB65_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78023.1"
                     /translation="MAKVAVEANKAVSIKAWMRRSNTELTMRLIVKGKQIAGVDNDIY
                     ADLTADANI"
     gene            4099..4293
                     /locus_tag="PLB65_05530"
     CDS             4099..4293
                     /locus_tag="PLB65_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78024.1"
                     /translation="MINATELLKMDYAFQGQPFVCVPASSSIDLKTMDYAYLAQPFFS
                     NYYIYTAIYTAMSNFFLFFN"
     gene            4581..4826
                     /locus_tag="PLB65_05535"
     CDS             4581..4826
                     /locus_tag="PLB65_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78025.1"
                     /translation="MDIVKLTNGQAVASGISILATTFNMKSTANTVVAKEGKDLSPNR
                     QLKPNDRLALKFTGVLTNLTGVQVTLYLKNLARGHYK"
     assembly_gap    4827..4926
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4988..5323
                     /locus_tag="PLB65_05540"
     CDS             4988..5323
                     /locus_tag="PLB65_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78026.1"
                     /translation="MWARLGDAEFYDDFMKTRPATPLSVFPGSYQYPHPMIVGDKDLL
                     YIGDGPRLHAFDGKYEGDDDGKFYNSVLTIPGTYYIKAFQKYKDFLLIFADENLSGTA
                     YSSNAKVFF"
     gene            complement(5395..5679)
                     /locus_tag="PLB65_05545"
     CDS             complement(5395..5679)
                     /locus_tag="PLB65_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78027.1"
                     /translation="MPELSDKAPDVDPPPFPIILSVCGNPTLLPKAYNLPLEFHLIWL
                     AATLTPPLTGSVSLKNMTGSKPVPVNNCILPVLESPVSYFLPEVKHPIFP"
     gene            5687..6019
                     /locus_tag="PLB65_05550"
     CDS             5687..6019
                     /locus_tag="PLB65_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78028.1"
                     /translation="MSSGTTTAGGLQSFSANYDYSAYFQTTSVEPDFGTRKKGRVKRV
                     KIIFGCTNVATADKITVKLYYDRGGSNVTIASGIAEITADTLVMQYEHDTSGNPLPDF
                     ESLSLRCD"
     gene            6273..6473
                     /locus_tag="PLB65_05555"
     CDS             6273..6473
                     /locus_tag="PLB65_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78029.1"
                     /translation="MKTVASQNYVNLPQDVDKLYTFYVDIDDQLYQATELASKDDFDS
                     LGVTNKCSYPLYYYVFDRKLYF"
     gene            6786..6986
                     /locus_tag="PLB65_05560"
     CDS             6786..6986
                     /locus_tag="PLB65_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78030.1"
                     /translation="MLPVYEAVMNYWAMQGEIGRTQTYKALYDEMYKLLKKEYSSKST
                     KVRVDTLTDKTVNNPNLYITGI"
     gene            complement(6990..7235)
                     /locus_tag="PLB65_05565"
     CDS             complement(6990..7235)
                     /locus_tag="PLB65_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78031.1"
                     /translation="MAFVLIVLAVGLYVGVESVVAFTSLVLFFVSVVGETGLTCLGVG
                     LNPNELATLNDLFIVGSNNLFIALFPKYCHQFAIFNI"
     assembly_gap    7443..7542
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7543..8138
                     /locus_tag="PLB65_05570"
     CDS             <7543..8138
                     /locus_tag="PLB65_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78032.1"
                     /translation="TIPTTTPVESETIPTTTPVESEADKYFKMYLESIGITPEEEALQ
                     KQLSNIITGADLGVAGLEGQGRGIPLSLVRGQQEKLLRQAAIQAQPLQKQLELLQAQR
                     QVKAEMAKAASEYYKPKETEYKPITIGAGETLIDPVTGKVIYQGAPKASDFKSVGAGE
                     TLIDPITGKVIYQGTPKTDYSNLKEVQGGLYNMATGE"
     gene            8235..8669
                     /locus_tag="PLB65_05575"
     CDS             8235..8669
                     /locus_tag="PLB65_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78033.1"
                     /translation="MEQAQPTLKNLENTIANTSSFAFNNYEKLPAELQPIAYRQFMQA
                     ARNFINAVLRRESGAVISPTEFAEARKQYLPQPGDNADILAQKEANRNIVYNSFKKGA
                     GSAYQSLDELLGNTAVKPQNQPQTMTLPNGAVLTLQPDGTYE"
     gene            8692..9096
                     /locus_tag="PLB65_05580"
     CDS             8692..9096
                     /locus_tag="PLB65_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78034.1"
                     /translation="MGYTYDELLKMGATPTDTAPTQKKKYTYEELTAMGAKPTQPTQT
                     TQPAQPSEDIALGLLIGAAQGAGSTLESRLRSVGQIAGGIGASISVPIQERRQADLVT
                     QYKQALQDKNPALAKQIYNQINQAGTMSRQAQ"
     gene            9730..9960
                     /locus_tag="PLB65_05585"
     CDS             9730..9960
                     /locus_tag="PLB65_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78035.1"
                     /translation="MNSLDESIENAKKGLDSFDPDVRTSAKLTVKKLQELKKNFTDYV
                     LKIGKGGIKTNPKFVPVALANDAKQRLGDVAK"
     gene            9997..10221
                     /locus_tag="PLB65_05590"
     CDS             9997..10221
                     /locus_tag="PLB65_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78036.1"
                     /translation="MAKVKAYGATRQAIENTVPEIIPVNEALSNYISARHALGKVMDA
                     AQQYKEPILSGAPLIKRGVGGLIGYSIKKD"
     gene            11404..11631
                     /locus_tag="PLB65_05595"
     CDS             11404..11631
                     /locus_tag="PLB65_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78037.1"
                     /translation="MGQSKTTILNHWCDALHEQINGNTTNAIDILQELVDILDKELET
                     VKKDKKEMENIIELKIKVKMLIDKLNHKNEN"
     gene            11839..12327
                     /locus_tag="PLB65_05600"
     CDS             11839..12327
                     /locus_tag="PLB65_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78038.1"
                     /translation="MEIAKEVQALIKRQAEGADKVAKEIIIKNQNDFEKASELFLKVK
                     QAGKYIASEKEKMTRGLLDSLANIRSFFKPYEIMQSNAEKEVGSKILAYQLEEEKKSQ
                     IKIDAVVEKVEKGQITFEQANEKIETLEVAKTVEVNTGAVQFKTVRDYEIIDEKLIPK
                     KY"
     gene            12541..12687
                     /locus_tag="PLB65_05605"
     CDS             12541..12687
                     /locus_tag="PLB65_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78039.1"
                     /translation="MDDNGNIPEKVQLIAFRNFFKTEDRQPDYRIYKSERREVSNDDQ
                     KFEK"
     gene            12777..13364
                     /locus_tag="PLB65_05610"
     CDS             12777..13364
                     /locus_tag="PLB65_05610"
                     /inference="COORDINATES: protein motif:HMM:NF016302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HOQ78040.1"
                     /translation="MKIQQALATIQKELKAPKGQYNAFGKYAYRSCEDILTALKKLLE
                     QTETVLILNDEVVNIGSRYYVKATATLSNGEETISATAYAREEEIKKGMDGSQITGAS
                     SSYARKYALNGLFGIDDTKDSDKTNQGFVGANKGIRQGSTSNFDKAKGIIEHTDDKNT
                     LEESIPKIVASEKYTDDEKEILVQMIENKIEQNEN"
     gene            13471..13935
                     /locus_tag="PLB65_05615"
     CDS             13471..13935
                     /locus_tag="PLB65_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78041.1"
                     /translation="MKKGKDLAEYLEKGGDVDDDIKCVADFIPQYKEKEYEIKVEMEG
                     IKLLGYLDSFDPKELNIYEYKTGKKYTQGMANKLGQLDFYTLMIYLKYGKLPRSIKLI
                     WIETETQDDNVVFTGKIKTFEVKKTMKDIIKISSRIKKAKQGIDEMWEMVNG"
     gene            14226..14663
                     /locus_tag="PLB65_05620"
     CDS             14226..14663
                     /locus_tag="PLB65_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78042.1"
                     /translation="MTKLKNEKCFASNKTLTDICGVKSEQAIRGALLRLEKQGYIQRV
                     YKDGKRKIRSEIIPLVVYSKVATNVATVATNVATNVATNVAQNKNIYNKNIKEELHTL
                     FDKEIQTDNLKEKENFLSYWTEKNTGGKKERWQKQTVFDIKRR"
BASE COUNT         5875 a         2206 c         2598 g         4107 t
ORIGIN      
        1 agcagttaat tcagcttggg ctgaaattaa aaaactaaaa gaaaaatatg atagatgata
       61 ataaaataca cgctacaatg actattacaa acgatgggaa taatactcca actgatatga
      121 tcgtagatga aactacaggc agattacttg ttaaaattgt agttgaagat attacaacag
      181 aaccacgaag tatagacata gacgccaatc aagagtatgt tgggcttgtt ttggcagatg
      241 atgatacaac aattgttcct ttaatggtaa ataaagatgg tgctatttac gctaacttag
      301 tagtagaata gtagatactt aattaaaaat taaataaata aaaatatgac tgatataaca
      361 tatccaatag acgataatca caatccaatc actgggttgg atatgatcga ttcgaaatca
      421 ataacttatg ttgccgcaac aacaggtgct gttggtgtta caaatctatt tactattata
      481 ggaacagtcg ctttacgaat attcggtgta tgttctgtta atttaacagg cagtggaaca
      541 ttagaagtcg gtattgcagg aatgactaat gggttgattg atcaaactac tggaactgat
      601 attgataaag atactatttg agttgataat gctcctgcta aaatagaaac acttccaagt
      661 ttatcaatac tgactgatca aacaattata caaacaatag gaactaatac tataacagcg
      721 ggaacgctaa cttactactg ctgctgaacc cctatatcaa gttctgcgag tgtggccgct
      781 gcttaataaa caattaaaat actactatgg ctgatgaatc ggcaaaaata gacgtaaatc
      841 aacaaaaaac attattagca gtgacaaatg ctgctacacc tgaaattaag aatgttagat
      901 gtgacgcaac tacaggcaga gttttagtat ctgctactgt ttctggtgtt tctgatatta
      961 cagtagctaa tgaggcgaca gatacttctt gctttccttt atttgctact gctgctacag
     1021 ggagtataca accaaaaaca aatgctggac taacatttaa ttcaaacaca ggaaacttgg
     1081 gtgcgacttt aatgaatact cacaagataa cagtcggtgt cgccggaact gctactggtt
     1141 caattgaact taatggaact acaagtggaa cagtgacttt gaaaactgct gatgccgctg
     1201 gaacttatac tcttacacta cctacaaacg atggaaatgc taaccaattc ttacaaacaa
     1261 atggcaatgg tgttttaact tgagcagata taactgttag tggtggtgct tctactgctt
     1321 tagataattt agtaaatgtt gctattaata ctaatttatt acctggaata gataatttta
     1381 tatctttagg agatactact catacttgaa aaactttgtt ttgtgaaaca atagaattag
     1441 gacatgattc tgatacaaca ttatcacgaa gtgctgctgg agtattagct gttgaaggag
     1501 tagttattcc aacaattagt tcggcagata ctttaactaa taaaagaata acaccacgag
     1561 tatattctga agcttctaat gctacaccta ctcctaatat tgattcctat gatatgtatg
     1621 aattaactgc tttagctgtc agtgctactt tttctgctcc aacaggaact cctactgatg
     1681 gtcaatcatt gattattaga attaaggata ataacaatgc acaatcttta agttggaatg
     1741 ctatttatag agcttcaaca gatttagctt tgcccactac aacaagtgct aataaaacaa
     1801 tgtatttagg ttttaaatat aatagcgcag attctaaatg ggatttgcta gcttacttaa
     1861 acaatatata aaaataattt tatggctata ttttatttag attatgaaaa tggaaatgat
     1921 gctgctcgct cttcagttaa tattacttct tctgataata atggttcagg acttgttaga
     1981 attttaactg ctgcaaatca cggattagtt actggagcaa aagtgacaat tgcaggacat
     2041 tcagtatcag aatataatgg tgattgggtc attacaaata tagatgctac tcattttgat
     2101 ttacagaatt caacctattc ttctagtggt acaggaggaa ctgaaactcc atttgggggt
     2161 atgaattggg ctgatgcttg gaaaacaata actaatggag caacagcagc cagaattgca
     2221 cctggtgata ttattagaat tgctaaaagt ccagcacctg tttctattgg tagtgctagt
     2281 tggactaatt tatctaaaac agtagttttg gaaaatgcac aaactttaaa tgtggatatg
     2341 tgcgaaaatg tttgaactgg tgtaaatgat acaactgtaa ccaggacatc agtaagtaca
     2401 gatgctaaag aaggttctta ttgtatgaaa ttagatcttg atagttctcc acaaacaaat
     2461 aaattacaag catattataa gatatctgat gatggaataa actttagtgc ttatcaaaaa
     2521 ttatcttttt gaattaaaaa ctctgcagca attgtggcta ataactgata tgtggcttta
     2581 tgctctgata ctgctggggc aacagtaata gatactttta agataccagc aattccatca
     2641 acagaaagat gaattcctgt gactttagca agagaaggaa gcggcaatct tggaaatgct
     2701 attaaatcaa ttgctgttta tacagatagt actgctccaa cagcgagttc atatatcttt
     2761 atagatgatt ttattgcctg cacaactaat gggcttaatt tacaaagttt aatttctaaa
     2821 aattcaaacg aacaaggagg aacagaagga tggtattgca taaagaatat aaatggaaca
     2881 actattttgt tagataatga tactaactcc aaagctaatg ctggaagagg atattatgga
     2941 acaacagaaa cagtaacaac ttataaaagg gaaacaatta aaactgcaat ggcaggtagt
     3001 tcttcaacag caattcagga agttaaagat agtggaactt caggaagcaa gatagaattt
     3061 caaggtggat ttaatacttc aaataataat caagaaggag aaacattttt tgatggatta
     3121 aatggatgag gatatggaat ttatttaaat ggcaaatctt atactattct taattatttg
     3181 aatgtttgta gatattataa tggaatttat tattataaca ttagtaacaa taatactata
     3241 actactctaa gtaatgctaa taataatagc aattatggaa tttattataa cagtagtaac
     3301 aataatacta taactacttt aggtaatgtt aataataata gtagtcatgg agtttcttac
     3361 gcccaaagta acaataatac tataactact ttaggtaatg ttaataataa tagtggttat
     3421 ggagtttatt acgaccaaag tagcaataat actataaaat ctctttcaac aagtggaaat
     3481 agtacaggag gagtttataa taatacagga gtaaattatt tattcaatgc taaaattcaa
     3541 gaagcaacag aagttgatgg atttcaaaat tttgccaatt ctcgtatttt ctctcaaaac
     3601 catgaccaaa tagcagataa ccattgaata tttactgatg gcgggacaat tcattctcaa
     3661 acagcagtaa gacacacagc ttctggtatt gcttgacagt taagtccgac ttctaccaat
     3721 agatcatcta actatccttt ggatttaagt gtggcaaaag tagcggtaga agcaaataaa
     3781 gcagtaagta ttaaagcgtg gatgaggcga agtaatacag aattaacaat gagattaatt
     3841 gtaaaaggaa aacaaatagc aggagtagat aatgatattt atgctgattt aactgctgat
     3901 gctaatatct gacaggaagt ttcattatca agttttactc caacagaaaa aggagttatt
     3961 gaggttatgg tatatgcttg aggtggcaca aactataatg gatttatcga tgatttaaca
     4021 atttcacaat cttaatttta aaactatgaa ttacaaaaga ttaaagcaaa ggtcaattag
     4081 aaaccaaaat aaaataaaat gattaatgca actgaattac taaaaatgga ttatgctttt
     4141 cagggacaac catttgtgtg tgttccagca agtagttcta ttgatttgaa aacaatggat
     4201 tatgcttatc tggcacagcc atttttcagt aattattata tttatactgc tatttatact
     4261 gcaatgtcta atttcttttt attttttaat taatatttat ggataataca aatgaacaag
     4321 aaaaacaaat aaaagaatta gaagaaagaa cagtcaattc tacacttatg gatgctaaag
     4381 atggattttt tattacaact tgatataatc agattatgtc tgattataca atcagtatag
     4441 attttaataa taaatcatac tcaggaactc aagtattagc acctgctaat ttaccatttt
     4501 ttatagcccg acatccttgt gaagtattat gagtagcaga agcacatata attgctggaa
     4561 gtgactctgg caatgtaact atggatatag ttaaactgac aaatggacaa gcagttgctt
     4621 ctggaatatc catactcgca actacattta atatgaaatc aacagcaaat acagtggtcg
     4681 caaaggaagg taaagattta tcaccaaata ggcaattaaa accaaatgac agattggctt
     4741 tgaagtttac tggtgtactc acaaatttaa caggagtaca agtaactctt tacttaaaaa
     4801 atctcgcaag aggtcattat aaataannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnntgtt tatccaagag tattttattc ttgaaattca acaactaaat ttagttgggg
     4981 tgttggaatg tgggcaagat taggtgatgc tgaattttat gacgatttta tgaaaacaag
     5041 accagcaact ccattaagtg tttttcctgg ttcttatcag tatccgcatc caatgatagt
     5101 tggagataag gacttacttt atattggtga tggaccaaga ttacacgcct ttgatggcaa
     5161 atatgaaggt gatgatgatg gaaagtttta taattctgtt ctcactattc caggaactta
     5221 ttatataaaa gcatttcaga aatataagga tttcttgctt atctttgcag atgaaaattt
     5281 atcaggaact gcttattcat ctaacgccaa agtattcttt tgagattact tatctcttga
     5341 tccgacatac ataatggact taaatgataa tttagtaaca gaagcattta cttttcaagg
     5401 aaagattgga tgtttcactt ctggaagaaa atatgataca gggctttcta atacaggtaa
     5461 aatacaatta tttacaggaa caggttttga accagtcata tttttcaatg atacacttcc
     5521 agttagaggt ggtgttaatg tagctgccaa ccagattaga tgaaattcaa gtggtaaatt
     5581 atatgctttt ggtaataatg ttggattgcc acaaacactt aatattatag gaaaaggtgg
     5641 aggatcaaca tcaggtgctt tatcagatag ttcaggcaca atacttatgt cgtcgggaac
     5701 aacaaccgct ggtgggcttc aatctttctc agcgaactac gattattcgg cttactttca
     5761 aacaacttct gttgaaccag atttcggaac cagaaagaaa ggtagagtaa aaagagtaaa
     5821 gataattttt ggctgcacaa atgttgcaac tgctgataaa ataactgtta aactttatta
     5881 tgatagaggt gggagtaatg taacgatagc aagcggaata gccgaaataa ctgctgatac
     5941 tctggtaatg caatacgaac acgatacgag cggtaatcca ttaccagact ttgagtctct
     6001 atcacttaga tgtgattgag ggtcagggac aattcatact tcagcacctt gaatatcatc
     6061 aatcgaagtc gaatacgaaa caattaatgc atagtttaat aaacaattaa aataaaaaga
     6121 tgttaactta tactggcagc agaaatttgg cggggtcgct aacaaacaat acgagcacaa
     6181 ccaacttgac tcttatggac actttaatta accaaagtat tagaaaaatc acttcaagcc
     6241 acgaatgacc ttttctcgaa aagacagcaa ctttgaaaac agtagcaagt cagaattatg
     6301 taaacttacc acaagatgta gacaaacttt acacatttta cgttgatatt gatgatcaac
     6361 tttatcaagc aacagaactg gcaagtaaag atgactttga ttcattaggg gttactaata
     6421 agtgttcata tccactttac tactatgtat ttgatagaaa actttacttc tgaccagagc
     6481 catcaacttc tgattatgac atcgtaatta cctataaaca aagggtaacc gatttagata
     6541 ccgctgacta cactactggc acaatagcta ttacaacagc cacagcaact gttgtaggct
     6601 caggaacgac atttgtagca gatatggtag gtagatactt aaaaacaact gatagaattt
     6661 gatacaaaat atcagctttt acagatgcga cacaccttac attagacaga acttatatcg
     6721 gatcgacaat aacaggggca agttatacaa taggtcaagt ttcaatctta ccagaggact
     6781 tccaaatgct accagtttac gaagcagtta tgaactattg ggcgatgcag ggagaaatag
     6841 gaagaactca aacttacaaa gcactttatg atgagatgta taaattactt aaaaaagagt
     6901 atagctctaa atcaacaaaa gttagggtcg acacattgac tgataaaaca gttaataatc
     6961 ccaacttata tataacagga atataaaaat taaatattaa atatggcaaa ttgatgacag
     7021 tatttaggaa ataaagcaat aaacaaatta ttactaccaa caataaataa atcattaagt
     7081 gtagcaagtt cattagggtt taagcctact cctaaacaag ttagtcctgt ttcacctaca
     7141 acagagacaa aaaacaatac taaagaagta aaagcaacga cgctttcaac tcctacatat
     7201 aatccgactg ctaatactat taatacaaat gccattcaaa atgtatctaa taatcctcca
     7261 agtaatcctc caagcacacc agatttatct tctctaatgt cgggattaca aaatttatct
     7321 cagggacttt cacaattaca aataccgcaa caacaaacta taccaacaac tacaccagta
     7381 gaaagtgaaa ctataccaac aactacacca gtagaaagtg aaactatacc aacaactaca
     7441 ccnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnaaactata ccaacaacta
     7561 caccagtaga aagtgaaact ataccaacaa ctacaccagt agaaagtgaa gcagataaat
     7621 atttcaaaat gtatttggaa agtataggaa taaccccaga agaagaagca cttcaaaaac
     7681 aactctctaa tataataact ggagcagatt taggtgtagc aggtttagaa ggtcagggtc
     7741 gtggtattcc actttcattg gtaagaggac aacaagaaaa acttcttaga caagcggcaa
     7801 tccaagccca acctcttcaa aaacaacttg aattacttca agcacagaga caagtaaaag
     7861 ccgaaatggc taaagcagca agtgaatatt ataaaccaaa agaaactgaa tataaaccaa
     7921 taacaattgg tgctggagaa accttaattg acccagtaac aggcaaagtg atttatcaag
     7981 gagctccaaa agcaagtgac ttcaaatctg ttggtgcagg agaaacctta attgatccaa
     8041 taacaggtaa agtcatttat caaggaactc caaagacaga ttactctaac ttaaaagaag
     8101 ttcaaggtgg tttatataat atggctaccg gtgaatgagt agtccaacca aaagcaacag
     8161 ggactggagg ggctcctaca tcttataaag caccaacaca agcagaacaa acattggcta
     8221 cttatgctgc aagaatggaa caagcacagc caactcttaa gaatttagag aacacaatag
     8281 ctaatacaag ttcttttgct tttaataatt acgaaaaact tccagcagaa ctacaaccaa
     8341 tagcttacag acaatttatg caggcagcga gaaactttat aaatgctgtt ttgagaaggg
     8401 agtctggggc agttattagt ccaacagaat ttgcagaagc cagaaaacaa tatctaccac
     8461 agccaggtga taacgcagac atattagccc aaaaagaagc aaatagaaat atagtgtata
     8521 attcatttaa gaaaggagct ggttcggctt accaatcatt agatgaatta ttaggaaata
     8581 ctgcagttaa accgcaaaac caaccacaaa caatgacctt gcctaatggg gcagtgttaa
     8641 ctctccagcc agatggaact tacgaataat aaaccaaata aataaaatat tatgggatac
     8701 acttacgatg aattattaaa aatgggtgct acaccaacag acacagcacc aactcaaaaa
     8761 aagaaatata cttatgaaga gttgactgct atgggtgcta agccaactca accaacacaa
     8821 acaacacaac cagctcaacc ttcagaagat attgctctgg gtcttttaat aggtgcggct
     8881 cagggtgcgg gatctacttt agaaagtaga ttaagaagtg ttggacaaat agcaggaggt
     8941 attggtgctt ctataagtgt tcctatacaa gaaagacgac aagcagattt agttactcaa
     9001 tataaacaag cactacaaga taaaaatcca gcattagcta aacagatata taatcaaata
     9061 aaccaagcag gtactatgtc aagacaagct caatgacttt ttgataatcc tttacttgct
     9121 aaagcgagag aagccttaac tccaaaaaca actgctgaaa aggtaggata tggtgcagaa
     9181 aagatacttg aattggtata tggtgctggt agttttgaaa ctatggcaaa aactgcacta
     9241 ccaaaagaaa caagtaatat aatcaaactt ggagaaaacc tcttaaaatc aaaaaatgct
     9301 tgaacaagag ttgggggagc aattctaaaa gattcaccac acgcagctgg aacaatatta
     9361 acaactcttg ttcaatctgg accaggaaaa actaaggaag aagtaaaaaa gaatgttacg
     9421 tcagctttaa tagctggtgg tttagattta gcaacaactg ctgtaattca aggtggtaag
     9481 gaagcaatcg acaggtatat aaaagataaa cctgctaaat gaataaataa actaataaac
     9541 gttaaaaagg gagatttaac ttatggtaaa aatccaggtg agcaaatagc aaaagataaa
     9601 ataagggcta atagtttaaa aagtttagaa gaacaggtca ccggaaaaat aaatgaatta
     9661 agcaataaag ggaaagatat aatatctaaa tcaaaaaatg ccaacgctac ggttgactta
     9721 actccaacat tgaattcgtt agacgagtca attgaaaacg ctaaaaaagg attagatagt
     9781 tttgatccag atgttagaac gtctgctaaa ttaacagtaa aaaaattgca agaattaaaa
     9841 aagaatttta cagattatgt attaaagatt ggtaagggtg gaataaaaac aaatcctaaa
     9901 tttgtccctg tcgctttagc aaatgatgct aaacaaagat taggtgatgt tgctaaatga
     9961 actcctgaag cagcttctta tatcaaagat attaacttgg caaaagtaaa agcatacgga
    10021 gctaccagac aagctattga aaatacagtg ccagaaataa ttccagtcaa cgaagcgttg
    10081 agtaattata tttctgcaag acacgcttta ggaaaagtta tggatgccgc tcaacaatat
    10141 aaagaaccaa tattaagtgg tgctccttta attaaaagag gcgttggtgg tttgattggt
    10201 tattctatta aaaaagattg aaagtgggca gttcttggtg gtctattaga accttatgtt
    10261 tatcaactgg cacaaagccc agcttttgtt actgctattt cttcggctgt gaaatcgatg
    10321 tcaccaagtg acgtttcaac tatagcaaaa aactctccaa cattgtggaa agaaataata
    10381 aaacaaggtt ttataggaaa agctacaggt caatattaaa aaagaatata aacaaattaa
    10441 atataaccag tgctaatata actgaaataa ttcacatatt tataatttag tattgagtat
    10501 attataaagt attttttgaa aaaagtcaag tgcaaaactc tcaaatgggt tttgctgggt
    10561 ggtggttgaa tgttatccac catccagcaa aggtcattta ataaaacctt aaacatatac
    10621 atatgacaaa attacagtca ttattaatat cttttaagaa gtccataaaa attgttgtga
    10681 tatttttagt cggcgttatc attgctggtc ttgaagtaaa gtatccaaca gttatgggtt
    10741 atacagtttt caaccaatat acaattggta tgatactcta tattatctat gacttcttaa
    10801 agcacaactg gatcacgaaa ttaccataaa cctcttgaca aaaaattaaa aattggtata
    10861 atatatatga tatatatata ttagtatact tacaatcaaa ataaataaat ttatataatt
    10921 tttttcccta ccatatgcaa tccgctcata gggtttttta ttgacatcta tgctatttta
    10981 tagcgtgggt gtcatttttt attgtattaa aaataatata aataaaatcg tatttataga
    11041 cccaaaaaag agaaataacg taaaaaaata aagccaaaaa gtatagtatt tactacataa
    11101 ttttgagtta tccacttttt atatcgtctt acctattgac aaaataaact taatatgata
    11161 gaataataat aagaatagaa aaaggtcgag aaattaaaaa acattaaaaa agcaacaatg
    11221 gtatttatat taatcttact aaatatactc gcactaaata atttacctta tggggaatcg
    11281 gttagtggcg tgtataaatc ggctggttgg ttgttgataa gtttatcgct cctaacagta
    11341 agggcgatca tagggacaat tcgttcagtc aaagaattaa aaaataacta aacaaaaaat
    11401 aatatgggac aaagtaaaac aactatactc aatcattggt gtgatgctct acacgagcaa
    11461 ataaatggta atactacaaa cgctattgat attttacaag aattggtcga cattcttgat
    11521 aaggaacttg aaacagtcaa aaaagacaaa aaagaaatgg aaaatattat agaattaaaa
    11581 ataaaggtta aaatgttaat agataaatta aatcataaaa atgaaaatta aaaaagaagt
    11641 aacgtgacaa gatgttgaaa aatatatcat taaaaattgt aaggtcaatg atgaattgtt
    11701 agccattagc gtaaatgact tgcttaaaag atatggcaca acaagacaga acttacatag
    11761 aaagttggat agaatactcg ataataagaa atataaaaag gtcggggagt atatcttaat
    11821 caaaattaaa taataataat ggaaattgca aaagaagtgc aagccttaat taaaagacaa
    11881 gcagaaggag cagataaagt tgcaaaagaa attattataa aaaaccaaaa tgattttgaa
    11941 aaagcttcag aattatttct aaaagtcaaa caagctggaa aatatatcgc atcagaaaaa
    12001 gaaaaaatga ctcgtggttt attagactca cttgctaaca ttagaagttt tttcaaacca
    12061 tatgaaataa tgcaatctaa tgcagaaaaa gaagtcggaa gtaaaatatt agcatatcaa
    12121 ttagaagaag agaaaaaatc acagattaaa atagatgcag tagttgaaaa agtggaaaaa
    12181 ggacaaataa cttttgaaca agcaaatgaa aagattgaaa cattagaagt agcaaaaaca
    12241 gttgaagtta atacaggggc tgtgcaattc aaaacagtta gagattatga aataattgac
    12301 gaaaaattaa taccaaaaaa atattgagta ttagatttag tcaaattaag aaaagacgct
    12361 ttagatggag ttgaaatacc aggcgtcaaa gtggtcgaga aaaaaataat aaattctaaa
    12421 cttaattaaa tggaaaataa tcaagagaaa aaacaaagtg ttggagcaat gtgaagtaag
    12481 gtaggtaaaa acgaaaaaca aacaagatat tactcaatat cacttgacac aaaacaaata
    12541 atggacgata atggtaatat acctgaaaag gttcaactca tcgcttttag aaactttttc
    12601 aaaacagaag atagacaacc tgattataga atttataaaa gtgaacgaag agaagtttcg
    12661 aacgatgatc agaagttcga aaagtgagtg aataaagaag acgaacctca acaagaaaag
    12721 ttagaagaac aagacgatga ccaaattcct ttttaataaa attaaattaa aacattatga
    12781 aaatacaaca agcacttgct acaattcaaa aagaattgaa agcaccaaaa ggacaataca
    12841 atgcgtttgg taaatatgct tatagaagct gtgaagacat tttaacagca ttaaaaaagt
    12901 tgttagagca aacagaaaca gttctgatcc taaacgatga agtagtaaat atcggatcaa
    12961 gatattatgt caaagcaact gccacattat caaatggaga agaaacaata tcggctactg
    13021 cttacgcaag agaagaagaa attaagaaag gaatggatgg tagccagatt accggagcca
    13081 gtagttctta tgcccgaaaa tacgccttaa atggcttatt tggcatagac gataccaaag
    13141 atagcgacaa gacgaatcaa gggtttgtag gggcaaataa ggggattaga cagggttcta
    13201 caagcaattt cgacaaggca aagggcataa ttgagcatac agacgacaaa aacacgcttg
    13261 aagagtcaat tcccaagata gtggcttctg aaaaatatac tgacgacgaa aaagaaatat
    13321 tggtccaaat gatagaaaat aagatcgaac aaaatgaaaa ttagacgaag ggactacttc
    13381 agttgaagcc aactcaacac ttgagaaaaa gacccaaacc tatattatga aatgtatgta
    13441 ttgggattgg aagcaccatt tacgaaatga atgaagaaag gaaaagattt agctgagtac
    13501 ttagaaaaag gtggtgacgt tgatgacgat ataaaatgtg tggctgattt cattccacaa
    13561 tacaaagaaa aagaatacga aataaaagtt gagatggaag gaataaaatt gcttggctac
    13621 ctcgatagtt ttgatccaaa agaattaaac atctacgaat acaaaactgg gaaaaaatac
    13681 acacaaggta tggcaaacaa attaggacaa cttgattttt ataccttgat gatttatctc
    13741 aaatatggta aactaccacg aagtataaaa cttatatgga ttgaaacaga aacacaagat
    13801 gacaatgttg tatttactgg taaaataaaa acatttgaag tgaagaaaac gatgaaagat
    13861 ataattaaga taagctcacg aataaagaaa gctaaacaag gtattgacga gatgtgggag
    13921 atggttaatg gttaataagt aggggttgac ttttttataa aaatatgata gtatatttgt
    13981 agattaaaaa tctgggacac aaaagcaatc acaaaaataa agaaacacaa acaaccagac
    14041 ctgatcattg tgatgctttg cgagagtagt gatactttcg caggtgtccc aatgctcggg
    14101 tctgttttaa taattaaaaa gcaaatatgg aaaacctgcc tgacttcatt caaataccac
    14161 atcaattaat aatggatcag aaactccgac cgacagatag aatactttat ggtgtgattt
    14221 actgaatgac aaaactaaaa aatgaaaaat gctttgcttc aaataagact cttactgata
    14281 tatgcggtgt aaaatcagaa caagctataa ggggtgcatt actacgatta gagaaacaag
    14341 gatatataca aagagtatac aaagatggaa aaagaaagat aagaagcgaa ataataccat
    14401 tagtagttta ctcaaaggta gcaacaaatg tagctaccgt agcaacaaat gtagctacca
    14461 acgtagcaac aaatgtagca cagaataaga atatatataa taagaatatt aaagaagaat
    14521 tacacacact ttttgataaa gaaatacaaa cagataactt aaaagaaaaa gagaattttc
    14581 taagttattg gacagaaaaa aatactggtg gtaaaaaaga acgctggcag aaacaaacag
    14641 tattcgatat taaaaggaga tgatctactt gacttaaaaa tagtaaaaaa tgatcgaatg
    14701 ataataaaca aggagaatga ggagaaagcg tctctgattt agacttagac tattataaaa
    14761 aacaaacaga aaaatggaaa caaagaaatc acatcgtata aaaacaaacc agatatcagt
    14821 aaaagaattg tttaggcttg tggtcgaaca atttaataaa aataaacaat ctaaaaatga
    14881 gaataaaaat aaacgagtta agaaatcagg tcatctcaat tctaagggct tatccagaaa
    14941 caagaaatag tgacataaca cttatgataa aaatatgaca ttcatt
//
LOCUS       DAOSTH010000038        14870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_4114_length_14870_cov_14.470942, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000038 DAOSTH010000000
VERSION     DAOSTH010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14870
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4114_length_14870_cov_14.470942"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(68..1390)
                     /locus_tag="PLB65_05625"
     CDS             complement(68..1390)
                     /locus_tag="PLB65_05625"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOQ78043.1"
                     /translation="MNSYNPVELIIKKRDAYELTAAEIAYFISAFLKGEITDYQMSAF
                     LMACFLQGLSETEIEALTEAYIASGNTLSFGKGLPVADKHSTGGVGDKISLMLAPIAA
                     ALGLLVPMISGRGLGHTGGTLDKLESIPGFQTSFSMQEFKKLVEKHGLALAGQSEELV
                     PADKKIYALRDVTGTVESPGLITASIMSKKIAEGALHLVIDLKIGSGAFMPNIRKANK
                     LAHLLLNTGKSFGQKVRVVFSNMNSPLGNAVGNALEMIEAIEYLKGNYLPDTYELTTE
                     LVTQMLFSTGKAKDYSQAKKMIDEAVLSGKALEKLQEIIIAQGGEPRVIDNYSLFPQA
                     NVSVPIIAKQNGWVQSIDSRTIGYALGHIKAGRQKVTDTLDYSAGALLYPKIGDEIRA
                     GDKIGEVFASHQSLAEEAARRILSAYTIADNKKDKERMIHKLIFSEDC"
     gene            complement(1401..2372)
                     /gene="phnE"
                     /locus_tag="PLB65_05630"
     CDS             complement(1401..2372)
                     /gene="phnE"
                     /locus_tag="PLB65_05630"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HOQ78044.1"
                     /translation="MIKKIKILSIEYAVWLYVIACISWFLLRLGNISFSGWIILLFPL
                     LIAFLFTFSKNCIGAKIIISEQPGALWKVEFAIVIIITLIMAWVIVEVKPIAFFTQAA
                     NTKAIVSGIFNPNLKELVPMLSALVETIYLALLATIFAIPFAFVLSFFAAKNLMNNSF
                     IGRIAYIIIRTVSTVVRSIEAIVWAIIFCVWVGIGPFAGMLALWIHSIAALVKLYSEQ
                     IENIDPGPVEAIKATGASTLQVWRYGVTPQILAPYLAFTIYRWDMNIRMATVVGFVGG
                     GGIGLALYQQQQMLAWRNVGLIMWLIALVVWVMDMFSGYIRSKLVTY"
     assembly_gap    2420..2519
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2668..2832)
                     /locus_tag="PLB65_05635"
     CDS             complement(2668..2832)
                     /locus_tag="PLB65_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78045.1"
                     /translation="MTDKTILIKELATRINKLLDIPIINEQNEQAFFEFMLSFLLNVF
                     LDELDKQLIK"
     gene            complement(2846..3298)
                     /locus_tag="PLB65_05640"
     CDS             complement(2846..3298)
                     /locus_tag="PLB65_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78046.1"
                     /translation="MKVNLKYGIKTYSGTLDGMTYGSYRKDTVCLGRKYVKPSLTNHN
                     VQMKAKMKNLSAIYAEVNSDYKVELKQYASKYAAKVPEGSLPPNAYALWIKMMFSFAS
                     NSEGHIELSTVTYNDLQTVGTDILSISTAVENGYLDSVPGANELIANM"
     gene            3482..4753
                     /locus_tag="PLB65_05645"
     CDS             3482..4753
                     /locus_tag="PLB65_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HOQ78047.1"
                     /translation="MDSSIKDLLNFLTGSRSSFLASLEIEKRLKAEGFSYLPEGNPFK
                     LKSGGKYYIRRSGTAVIAFVVGSEPVAKVGFNLAGSHIDYPCLKLKPQSIKTDKGITK
                     IGVQVYGSPIISTWLDRELGIAGKVVVKTAKGYKVESVDFKKPVAIIPNVAIHLNREV
                     NKGFSYNAQTQLNALLSVSASAENPLYNAIAEELKIKEEQILETELFLYDFAPAQLIG
                     LNQEMITAQGLDNLSMTHCILSALLKCDKPQKTAVGIFFDNEEIGSLTSQGADSLLLD
                     EILERICLSQSNSREDFYLALRNSFFISADVANAWHPSFSEKYDPDYAPLMNKGPVIK
                     FDAYSRYASNAESSYHFIHLCEKVKVPYQKFVVRSDGTGGITIGPILSARLGLNTVDI
                     GNPIWAMHSIRETGGIEDHLYLIKVLSQYFG"
     assembly_gap    4776..4875
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4887..6137
                     /locus_tag="PLB65_05650"
     CDS             4887..6137
                     /locus_tag="PLB65_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HOQ78048.1"
                     /translation="MATFKPFKALRPVQDKAKAIASLPYDVMDSDEARLEVQKNPLSF
                     LHVEKPEVDLPSGTDLYDPAVYAKARENLYKYISEGYMKQDAKPAYYIYKQVMDGRAQ
                     IGLVGLTSVDEYMDGTIKKHELTRAEKEADRIRHIDTCDAHPSPVFFTYRHQDIIDQT
                     VAKVMANKKPEYDFTSDEGIGHTLWAMDDPLDIKTIQNAFASLPNLYVADGHHRTASA
                     AKVGLKRREQFPNYTGDEEFNFFMAVIFPDNQLKIYDYNRVVKDLNGLSKEEFLAKVS
                     EKWNVTPIPEGENFAPQKKHTISMYLDGKWYRLEPKPGTWNEKNIVEDLDVSILQNNL
                     LNPILGIKDPRTDQRIDFIGGIRGLGELVKRVDSGKEVVAFAMYPTSMNELIGIADAG
                     EIMPPKSTWFEPKLRSGLFIHLLQ"
     gene            6134..7462
                     /locus_tag="PLB65_05655"
     CDS             6134..7462
                     /locus_tag="PLB65_05655"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HOQ78049.1"
                     /translation="MIKYTIISLGCPKNLVDSECFASIMESYKMQRTDAWEEANLILI
                     NSCSFILAALEELNSLLVDVINATDVRKTKIVVTGCIMNRGYEEFKESYREVDAWIPL
                     KDFVAFDQYLQDSFLFPKVTPQKLSYKQRTHLEDGKYVYLRIADGCNNKCSYCMIPYI
                     RGKQISEPIETLIEEAKQMQDYGNELVLVAQDTCSYGTDIYGEKALPKLIEALHNQTN
                     YDWIRILYLHPDHFDLEWIELWKKYPKLLPYFDIPIQQVSPKIIKAMNRKKSYQELKE
                     LFLDIQNEIPNAVFRTTLMVDFPGETNEDLELLDKFLAEVPILQGGVFNYSPESKVVN
                     DSIYAKYDWERGKNLMFTWEDKLNQEKEQLLEKYVGTVQPVLIEGYDPLSEQFVGRLW
                     FQAPEIDGCVYIDELPETKSSIVNVEIVDVIGEDVMSIFHSEIETPKGKK"
     gene            7463..8458
                     /locus_tag="PLB65_05660"
     CDS             7463..8458
                     /locus_tag="PLB65_05660"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOQ78050.1"
                     /translation="MKKIALTGIKPTGNPHIGNYLGAIKPALKLSETCEARYFIADYH
                     ALNACKDPIELRKMTVEIAAAWLACGLDPKKTLFYRQSDVPETFELLTILMAFTPKGL
                     MNRAHAYKAMVQNNLEASKDPDDGVNMGLYTYPILMAADILLFDTDLVPVGTDQFQHI
                     EMAQDIAQSFNYVYKTQAFKIPQPIAAEESKTIVGLDGRKMSKSYNNTIPMFCTEKEL
                     KKVVMSIVTNSQTVEEPKDPDSSNIFALYKNFATPEQIENLRARYRKGGLGWGQAKKE
                     LFELINAEFTPIREKYNDLMKDQNQIWNTLKEGSEKASKLASEKIKFLRNIIGID"
     assembly_gap    8480..8579
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8694..9104)
                     /locus_tag="PLB65_05665"
     CDS             complement(8694..9104)
                     /locus_tag="PLB65_05665"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOQ78051.1"
                     /translation="MTWTFTKRIYGYECDIYGHLNNANYLQLLEAARSEALYKMNLAQ
                     SDLQEKGVQLLVHKVEMNYIKAIDHDEIVTVKSRFVEINRIKGIWIQEIFNAQEEKCF
                     EANLTIVFASKGKAKRLPVEVYELFLPYIEEKQD"
     gene            9204..11717
                     /locus_tag="PLB65_05670"
     CDS             9204..11717
                     /locus_tag="PLB65_05670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HOQ78052.1"
                     /translation="MAAKNVNQKIKQNIRKSTKQSTSATKTPEVKTPSAFSKHLPWVL
                     VVILFLLLSLVYFPVAYQGKAPQASDITQWQGAAKAIIDYNATHKDNALWTPYMFSGM
                     PTYMISFPNRYPFLESITKLTDKIINWRIFLLFIGGLGIFLLLRQLKMDPWIAFFAAI
                     SFTFSCHWVGLLDIGHNTKFRAIMYIPWVVWALFRLKEKPNMLNLGLLATFLITQLRE
                     NHPQITYYLYLFIGMFWIYALIEAIKDKQYKKLGNWTLLLIIAFVLTALAVMNPYLST
                     WEYSHYTMRGGAAGLEKSYAQAWSFPPLEIIALLIPNFFGGINEYYWGAMPFTQIYNY
                     FGIVVLALGVIALFGKHKKFSLFLWIASAIFMLLSFGSFTPVLSDFFFKYLPMFNKFR
                     VPSMTLIMVQFIAVILAALGLDTIATLNNNSRWQKNLFTAFWVSGAVFFLFLILGKSI
                     FSGLPFTNAEEIARYQTKNALAQLESLKTMRLNMLYKSGIMSLLLLTVSFGICYLASA
                     KKLKTVALVMLITLISFIDLYIYTGKHLKDLYPAEEREARFVAQDFDQFLLEDKDNYR
                     IYPFNMGRVRTAGEWAYYHQTIDGYSAAKLKRYDDIWKLIQGDAKNDGEFLRYLIGVY
                     EKGGIETPTPILDMLATRYIVFPDSLPYASLLTKIRPVFSSYTGANIYQNLTAYPRAW
                     FVDSLSVIPDIKTRLENMQDLSFNPRSLAIVETKIDGVSKPDSCFAKETFFDMHNVKY
                     DVGTDKDAFLVVSEIYYPAGWKAFIDGKETEIYPVNHILRGVKIPAGKHTLEMKFISE
                     TYRLSLILSLSGILLTVILTVVGFAWEEKNKKRKINEIV"
     gene            complement(11839..14490)
                     /locus_tag="PLB65_05675"
     CDS             complement(11839..14490)
                     /locus_tag="PLB65_05675"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HOQ78053.1"
                     /translation="MEISKTFEPKDLEKKWYEFWEENGYFKPQENSTKKPFTVLMPPP
                     NVTGILHIGHVLNNTLQDVVVRYHRMKGEPTLWLPGVDHAGIATQNMVEKDLAKQGIS
                     RQDIGRDKLVEKIWQWKNEKGNIIIDQLKLLGASCDWERLRFTMDDMLSRAVKEVFVT
                     LYEEGLIYKGKYIINWCPRCVTALANDEVEHTDENGHLWYIHYPFADGNGYVTVATTR
                     PETMLGDVAVAVNPKDERYLSLLGKELLLPLTDRKIPIIADEYVDKDFGTGCVKVTPA
                     HDPNDFEIGVRHNLPQILVMDETGIMNANAGKNFEGLDRYACRETVLQMLSEQNLLEK
                     TETHPYSVGRCYRCDTVIEPYLSDQWFVKMAPLAKKAIEVVENGEVKFQPERWTKVYM
                     HWMNNIRDWCISRQIWWGHRIPVYYCDSCNNMIVAKAMPEKCPQCGGTKFTQDSDVLD
                     TWFSSWLWPFSTMGWPDETADLKYYLPTNVLITAPEIIYLWVARMIMSTLHFRDKIPF
                     DTVLLHGTVRDDIGRKMSKSLGNSPDPIDLINSVGADALRFSMIFGTPKGADVVFSES
                     ILETGRNFANKMWNAYRFIMMNAENIEGLPQENELQLELADRWIISRLQEVINEVREH
                     YENLRFNDAANAIFKFMWDEFCSWYIELSKDRLNPSAEKESKLTAKYILLNVMQNGMR
                     LLHPIMPFITEEIWQGIKKIFPMEEEALIVAAFPEADESLIDNDIDRNMSFVQESITA
                     IRNLRKQINLAPAQEIDIVIRFAEKSQQELFENYMAYFNRLAKVKTLSGGINIEKPKA
                     AIASVVRNIEIFLPLSGLVDIESEKNRLSKQIEKLAKELNGIQAKLANPNFLNNAKEE
                     IVAKEKEKFTEVNTKLELLKNLLEELL"
BASE COUNT         4473 a         2770 c         2763 g         4564 t
ORIGIN      
        1 gtactactct acattcgctt caatcttaga atattatctc atcctgaagg gaaagggttt
       61 atataagtta gcaatcctca gaaaaaataa gcttgtggat cattcgttct ttgtctttct
      121 tgttgtccgc tatagtatag gcacttaaaa tccgccttgc tgcttcttcc gctaagcttt
      181 gatgggaggc aaagacctcg ccaattttat ctcctgctct tatttcatcc ccgattttag
      241 gataaagcaa agcgccggcg ctataatcaa gagtatctgt aactttttgt ctgccagctt
      301 taatgtgtcc taaagcatag ccaattgtgc ggctatcaat gctttgtacc caaccgtttt
      361 gtttagcaat aataggaacg gatacattag cttgcggaaa aagagaatag ttatcaatta
      421 ctcttggttc tcctccctgg gcaataataa tttcctgtaa tttttccaat gctttgccgc
      481 ttaagacggc ttcatctatc attttttttg cctgggaata atctttcgct ttgcctgtgc
      541 tgaaaagcat ttgggtaaca agctcagtag taagttcata agtatctggc agataattcc
      601 cttttaaata ttcaattgct tcaatcattt ctaaagcatt gcccacggca tttcctaatg
      661 gcgaattcat attggaaaaa acaactctga ccttctgacc aaagctcttt ccagtattta
      721 gcagtaaatg agccagttta tttgcctttc taatattcgg cataaaggca ccgctaccga
      781 ttttgaggtc aataaccaga tgcaaagcac cttcagctat ttttttgctc ataatgctgg
      841 cggttattaa gccggggctt tctaccgtgc cggtaacatc acgcaaagca tagattttct
      901 tatcggcggg aacaagctct tcggactgtc ctgctaaagc caaaccgtgc ttttccacca
      961 gctttttaaa ttcctgcata gaaaatgagg tttggaatcc gggaatggat tccagtttat
     1021 ctaaggtgcc acctgtatgt cccaaaccgc gtcccgaaat catcggaacc agcaaaccta
     1081 aagcagcggc aatgggagcc agcatcaaac tgattttatc tcctactccg ccggtggagt
     1141 gtttatcggc gacaggaagc cctttcccaa agctgagagt attcccggaa gcgatatatg
     1201 cttccgttaa tgcttctatt tctgtttccg aaagcccttg taaaaaacaa gccattaaaa
     1261 aagcgctcat ttggtaatca gtaatctctc cttttaaaaa ggcactgata aaataggcaa
     1321 tttccgctgc agtaagctca taagcatccc gctttttgat gattagttct accggattat
     1381 aggaattcat attgtaacct ctaatatgta acaagtttgg aacggatata accgctaaac
     1441 atatccataa cccaaacaac taaggcaatt aaccacatta ttaaacctac atttcgccag
     1501 gcaagcattt gctgctgttg ataaagggca agaccaattc cacccccacc tacaaaacca
     1561 actacagtag ccatccgaat gttcatatcc caacggtaaa tagtaaaagc taagtaagga
     1621 gccaaaatct ggggagtgac tccataacgc cagacctgta aagtggaagc acccgttgcc
     1681 tttattgcct ctacgggtcc gggatcaata ttttctatct gttcactgta aagttttacc
     1741 agcgcagcaa tggaatgtat ccacaaagct aacattccag caaatggacc aatgcccacc
     1801 caaacgcaaa aaataatcgc ccaaacaatt gcttctatgc ttctgactac cgtggaaact
     1861 gttcggataa ttatatatgc aatccttccg atgaaggaat tattcattag attcttagct
     1921 gcgaaaaagg aaagcacaaa agcaaaaggt atggcaaaaa tcgttgccag caaagccaga
     1981 tagatggtct ctaccaaagc ggatagcatc ggaactagct ctttcagatt gggattaaaa
     2041 attccgctaa ctatagcctt ggtgttggct gcttgcgtaa aaaaagcaat gggcttaact
     2101 tctacaatca cccaagccat aattaaagtg atgataataa ctatggcaaa ctcgaccttc
     2161 cacagcgcac cgggttgttc agaaataatt attttggcac caatacaatt tttgctaaaa
     2221 gtaaacagaa acgctatcag cagcggaaat aagagtatta tccaaccgct gaaagaaata
     2281 ttgcctaaac gcaataaaaa ccagctgata caggcaatta catataacca tacagcatat
     2341 tcaatgctca aaatcttgat tttctttatc ataatgcaat cctcatttat gggtttgtag
     2401 agtcaaggaa aaagtgaaan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2521 ccggctacac ttcttgctga ggaacaaagt tatcatagtg ttttctgact tcttatttaa
     2581 ttctgacttc ttatttaatt ctgacttcgt atataaaggg aaaaagcccc ggcagggcac
     2641 cggggcaggt ggttctgtta atacagttta cttgataagt tgtttatcca attcatccag
     2701 gaatacattg agcagaaagg agagcataaa ttcgaagaat gcctgttcat tctgctcgtt
     2761 aattatagga atatccagaa gtttattgat acgggtagcc agttctttta ttaatatagt
     2821 tttatcggtc attttacatc tccttttaca tattagctat tagttcattt gcaccaggga
     2881 cactatccaa atagccattt tctacggctg tagaaataga caggatatca gtgccgacag
     2941 tttgcagatc gttataagtt acagtgctta gttcaatgtg tccttcgcta ttactggcga
     3001 aactaaacat cattttaatc cagagggcat atgcattggg aggaagagaa ccctcgggga
     3061 ctttagctgc gtacttagaa gcatattgtt taagctctac tttataatca ctgttaacct
     3121 ctgcataaat agctgataga tttttcattt tagctttcat ctggacattg tgattagtga
     3181 gactgggttt tacatatttg cgaccgaggc agacagtatc tttacgataa ctgccatagg
     3241 tcattccgtc taaagtgccg gagtaggttt taattccata ctttaagttt actttcattt
     3301 taagctccta tgcttatttt tttcttaggc gtttccggaa atctgccttc atatatatag
     3361 ggggtgacaa aactgagaaa gtggtgacaa aattcagaaa tatttttaaa aattacctca
     3421 cttgacacaa aagcaggtat ttaaaatatg gcattaatat gtataactat aggagttagc
     3481 tatggatagc tcaataaagg atttattaaa tttcctcact ggtagcagaa gcagttttct
     3541 agcaagtttg gaaatagaga aacggttgaa agcagaaggt tttagttacc tgcctgaagg
     3601 aaaccctttt aaacttaaaa gtggtggcaa atattatatt cggcgttctg gaacagcggt
     3661 tattgctttt gtagttggta gtgaaccagt tgccaaagtt ggctttaatc ttgctggaag
     3721 ccatattgac tacccctgtc tgaagcttaa acctcaaagc ataaaaacag ataaagggat
     3781 tactaaaatt ggtgtgcagg tttatggctc acctattata agtacttggt tagatcgcga
     3841 acttggcata gccggcaaag tggtcgttaa aaccgcaaag ggttataaag tggaatctgt
     3901 ggattttaaa aaacctgtgg ctatcattcc caatgtggca attcacttaa accgtgaagt
     3961 aaataagggc tttagctata acgcacaaac gcaattaaat gctcttttaa gcgttagtgc
     4021 ctcagctgaa aatccacttt ataatgccat tgcagaagaa ttgaagataa aagaagaaca
     4081 aattttagaa acagagctgt ttttgtatga ttttgctccg gcacaactta ttggattaaa
     4141 tcaagaaatg attactgcgc agggactgga taatctatcg atgactcatt gcattctatc
     4201 tgccttgctc aaatgtgata aacctcaaaa aactgctgta ggtatctttt ttgataatga
     4261 ggaaattggt tcacttactt cccagggagc agattcgtta ttattggatg aaattttaga
     4321 aaggatttgc cttagccaaa gtaacagtag agaggatttt tatctggcgt tacggaatag
     4381 cttttttatt agtgcagatg tagctaatgc ttggcatcct tctttttccg aaaaatatga
     4441 tccagattat gctcctctga tgaataaagg acccgtgatt aagtttgatg cttatagcag
     4501 atacgcatca aacgcagaaa gttcttatca ctttatccat ctttgtgaaa aagtaaaagt
     4561 tccttatcag aagtttgttg tgcgcagtga tgggacaggt ggaattacca ttgggcctat
     4621 tttgtctgct cgtttaggac tcaatactgt tgatatcgga aatccaatct gggcaatgca
     4681 ttctattcgt gaaacaggtg gaatcgaaga tcacttgtat cttataaaag tgcttagtca
     4741 gtattttgga tagcgaaaaa gcgagatagc gagatnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnaggag tatacaatgg caacattcaa accttttaaa gccctaagac
     4921 ccgttcagga taaagctaag gcaatagctt cattgcctta tgatgttatg gattcagatg
     4981 aagcacgcct ggaagtacaa aaaaaccctt taagctttct ccatgtagaa aagccagaag
     5041 tggatcttcc ttcaggaact gatttatacg atcccgcagt ttatgccaaa gcccgagaaa
     5101 acctctataa atatatatcc gaaggatata tgaaacagga tgcaaaacct gcttattaca
     5161 tatataaaca agtaatggat ggtcgtgccc aaatcggttt agtaggttta acttccgttg
     5221 atgaatatat ggatggcaca atcaaaaagc atgaattaac gcgtgctgaa aaagaagcag
     5281 accgcattcg tcatattgat acctgtgatg cacatccttc acctgtattt tttacttacc
     5341 ggcaccaaga tattattgac caaactgtag ccaaagttat ggcaaataag aaaccggaat
     5401 atgattttac cagtgatgag ggtattggtc acacgctatg ggcgatggac gatcctttag
     5461 atataaaaac tattcaaaat gcctttgcta gtttaccaaa tttatatgta gcagatggac
     5521 atcacagaac tgccagtgcg gcaaaagttg gacttaaacg acgggagcag tttcctaact
     5581 atactggtga cgaagaattt aactttttta tggcagtgat ctttccggac aatcaattga
     5641 aaatctacga ttacaatcgc gtagtgaaag atctgaatgg tttaagcaaa gaagaattct
     5701 tagccaaagt atctgaaaaa tggaatgtta ctcccatccc tgaaggcgaa aactttgctc
     5761 cgcaaaagaa acacactata agtatgtatc tggatggaaa atggtatcgt ttggaaccta
     5821 aaccaggaac ctggaacgaa aaaaatattg tggaagacct ggatgtttcc attctgcaaa
     5881 acaatctgct aaatcctatt ttaggcatca aagaccctcg caccgatcaa cgcattgatt
     5941 ttatcggtgg aattagaggt ctgggtgaat tggtaaaaag agtagatagt ggaaaagaag
     6001 tagttgcttt tgcaatgtat cctacaagta tgaatgaact tataggcata gctgatgcag
     6061 gagaaattat gccccccaaa tctacctggt ttgaacctaa attacgcagt ggactcttta
     6121 ttcacttact tcaatgatca aatatacgat tatcagtttg ggctgtccca aaaatttagt
     6181 ggatagcgaa tgctttgcct ctattatgga aagctacaag atgcagagaa ccgatgcttg
     6241 ggaagaggca aatttgattc tgattaacag ctgttccttt atcttagccg ccttggaaga
     6301 attgaatagt ttattggtgg atgtcatcaa tgctacagat gtaaggaaaa ctaagatagt
     6361 tgttaccggc tgtataatga accgtgggta tgaggaattt aaagaaagct atcgggaagt
     6421 ggatgcttgg atccctttaa aggactttgt cgcttttgat cagtatctgc aagatagttt
     6481 tctttttcct aaagtaactc cccaaaagct atcctataaa cagagaactc atctggaaga
     6541 tggaaaatat gtttatctgc ggatagccga tggctgcaat aataaatgct catattgtat
     6601 gattccgtat attaggggaa aacagatttc cgaaccgatt gaaactctta ttgaagaagc
     6661 aaaacaaatg caagattatg gaaatgagct tgttttagtg gctcaggata cctgttctta
     6721 tggaaccgat atttatggag aaaaagcgct tcccaaactg attgaggcgt tgcataatca
     6781 aacgaattat gattggattc ggatccttta tctgcatcct gatcactttg atcttgagtg
     6841 gatcgagctc tggaaaaaat atcctaagtt attgccctac tttgatatcc caattcaaca
     6901 ggttagccct aaaatcatca aagctatgaa tcgaaaaaaa agctatcagg aattgaaaga
     6961 actcttcctg gatattcaaa acgaaatacc gaatgctgtt tttcgaacta ctttgatggt
     7021 tgattttccg ggtgaaacaa atgaggactt agaacttttg gataaatttc ttgccgaagt
     7081 gcctatccta cagggaggcg tattcaatta ttctcctgaa tcaaaagtag tgaatgatag
     7141 tatctatgct aaatatgatt gggaaagagg caagaacttg atgtttactt gggaagataa
     7201 actaaatcaa gaaaaggaac agcttctgga aaaatatgta ggaaccgttc aaccggtctt
     7261 gatagaagga tatgatcctt taagcgaaca atttgttggt cgcttatggt ttcaagcacc
     7321 agaaattgat ggttgcgtat atatagatga acttccagaa actaaaagtt ctattgtgaa
     7381 tgtggaaatt gtggatgtta tcggagagga tgtaatgagt attttccatt ccgaaatcga
     7441 aacacctaaa ggaaaaaaat aaatgaaaaa aatagccctt acaggaataa aacctacagg
     7501 aaatccgcat ataggaaact atttgggagc cattaaacct gccttaaaac tttctgagac
     7561 ctgtgaagcg cgttatttta ttgccgatta tcatgcttta aacgcttgta aagaccctat
     7621 cgaactaagg aaaatgacag tagaaattgc tgcggcttgg ctcgcttgcg gcttagatcc
     7681 taaaaaaact cttttttatc gccaaagtga tgttccggaa acatttgagc ttttaactat
     7741 ccttatggct tttactccca aaggtttaat gaatcgtgcg catgcctata aggctatggt
     7801 ccagaacaat ctggaagcga gtaaggatcc cgacgatggt gttaacatgg gtctatatac
     7861 ttatccgatt ttaatggcgg ccgatatttt acttttcgat acagatttag ttccggtagg
     7921 tacagaccaa tttcaacata ttgaaatggc acaagatatt gctcagagtt ttaactatgt
     7981 atataaaacg caagcattta agattcctca accaattgcc gctgaagaaa gcaaaactat
     8041 tgtgggactt gatggacgca aaatgagcaa aagttataat aacacaattc caatgttttg
     8101 caccgaaaaa gaactgaaaa aagtggttat gagtattgtt accaacagcc aaaccgtgga
     8161 agagcccaaa gatccagata gctcgaatat ttttgctctg tataaaaatt ttgctacacc
     8221 tgaacaaata gagaatttaa gagcgcgtta tcggaaagga ggtttgggtt ggggacaggc
     8281 aaagaaagaa ctctttgaac taataaatgc agaatttact cctatccgag aaaagtataa
     8341 cgatctaatg aaagatcaaa atcaaatctg gaatacctta aaagaaggaa gtgaaaaagc
     8401 aagtaaactc gcctcggaga aaattaaatt cctaagaaat attataggaa ttgactgaga
     8461 taggattatt ataggattan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     8581 taatctgcta atccgttata atagcaatgc gggcaaaaga tagctatttc ctcttttgca
     8641 gctaaactat atcccctttt tattctgcga taagcaatgt tgtactattt gaatcaatct
     8701 tgcttttctt caatatatgg aagaaagagt tcataaactt caacgggaag gcgtttggct
     8761 tttcctttag aagcaaaaac aatggttaaa ttggcttcaa agcatttttc ttcttgcgca
     8821 ttaaaaattt cttgaatcca tatccctttt attcggttaa tttccacaaa gcgactttta
     8881 acggtgacga tttcatcgtg gtctatagct tttatatagt tcatttccac tttatgaacc
     8941 aaaagttgaa cacctttttc ctgtaaatcc gattgagcca gattcatttt atataaagct
     9001 tcgcttcttg cagcttctaa caattgcaga tagttggcat tgtttaaatg accgtaaata
     9061 tcgcattcat agccatagat tcgtttagta aaagtccaag tcatattatt caatcctttt
     9121 tatccagtat tttagctgaa atttacttga ccgatattcc tgtttatata attatggaaa
     9181 aaagaatata atcagaggat attatggcag cgaaaaatgt caaccagaaa ataaagcaaa
     9241 acataagaaa atcaactaaa caatctactt cagctacaaa aacaccggag gtaaagacgc
     9301 cttccgcttt cagtaaacat ctaccttggg ttttagtagt tattcttttt cttttgttaa
     9361 gtttagttta tttccctgtt gcctaccaag gaaaagcacc tcaggcatca gatataactc
     9421 aatggcaagg tgcggccaaa gccattattg actacaacgc aacccataaa gacaacgctc
     9481 tttggacacc ctatatgttt tccggaatgc caacttatat gatttctttt ccgaatcgat
     9541 atccttttct ggaaagcatc actaagctga ccgataaaat tatcaactgg cgtatctttc
     9601 tgctttttat tggaggttta ggcatctttt tacttttaag gcagctgaaa atggatccct
     9661 ggattgcctt ttttgctgct atatccttca ctttttcctg tcattgggtt ggccttttag
     9721 atattggaca taataccaaa tttcgtgcaa ttatgtatat tccttgggta gtttgggctc
     9781 tctttcgttt aaaggaaaag ccgaatatgc tcaatttggg cttgctggca acttttctga
     9841 ttacacaatt aagggaaaat catcctcaaa taacttatta tctatatctt tttatcggga
     9901 tgttttggat ttatgctctt atagaagcca taaaggataa gcaatataag aagcttggga
     9961 attggacttt actgctgatt attgcttttg ttttaaccgc tttggcagtg atgaatcctt
    10021 atctatctac ttgggaatat agtcattaca caatgcgagg gggggctgca ggtttggaaa
    10081 aaagttatgc tcaggcttgg agttttcctc ccttagaaat aatcgcactg cttattccca
    10141 atttctttgg gggtattaat gaatattatt ggggtgcgat gcccttcacc caaatatata
    10201 attactttgg aatagtggtg ttagctttgg gtgttatagc tctttttggc aaacataaaa
    10261 agttctccct ctttttgtgg attgcttctg ctatttttat gctgctaagt tttggcagtt
    10321 ttacgcctgt gctttcggat ttctttttta agtacttgcc gatgttcaat aaattccgcg
    10381 tgccttctat gacattgatt atggttcaat ttatagcagt tatccttgct gctttaggtt
    10441 tggatacaat tgccaccttg aataacaaca gtaggtggca gaagaactta tttacagctt
    10501 tttgggttag cggagctgtc tttttcctgt ttttaatttt gggtaaaagt attttcagcg
    10561 gtctgccttt tactaatgct gaagaaattg cccgttatca aactaaaaat gctcttgccc
    10621 aattggaatc gcttaaaact atgcgtctta atatgcttta taaaagcgga attatgagtt
    10681 tattgctgtt aaccgtatct tttggaattt gctatcttgc cagtgccaaa aagctaaaaa
    10741 ccgtagcttt agtaatgttg attaccttaa tatcctttat tgacctctat atatacactg
    10801 gcaaacattt aaaagacctc tatccagcag aagaaagaga agctcgtttt gtagctcagg
    10861 attttgatca atttctactg gaagataaag acaactatcg catttatcct tttaatatgg
    10921 gacgagttag aactgctgga gaatgggctt attatcatca aacaattgat ggttatagcg
    10981 cagccaaact aaaacgctat gatgatatct ggaaattaat ccagggagat gcaaaaaatg
    11041 atggcgaatt tcttcgctat ttaataggtg tgtacgaaaa aggtggaatt gaaacaccta
    11101 cccccattct ggatatgtta gctaccaggt atatcgtctt ccctgattct ttaccctatg
    11161 cttctctttt aactaaaatc aggcctgttt ttagcagtta taccggtgcc aatatctatc
    11221 aaaatttaac tgcttatcct cgggcttggt ttgtagattc actaagtgtt attcccgata
    11281 ttaaaacccg tctggaaaat atgcaggatt tatctttcaa tccgcgttct ttagctattg
    11341 tagaaactaa gatagatggc gtttctaaac cagatagctg tttcgcgaaa gagacctttt
    11401 ttgatatgca caatgtgaaa tatgatgtag gaacagataa agatgccttt ttagtcgtct
    11461 ccgaaattta ttatcccgct ggctggaaag catttataga tggcaaggaa accgagattt
    11521 atcctgtaaa tcatatttta agaggcgtta aaatccctgc aggtaaacat actttggaaa
    11581 tgaaatttat ctctgaaacc tatcggttaa gcttaatttt aagcttatct ggaatcttgt
    11641 taacagttat tctaacggtt gtaggttttg cttgggaaga gaaaaataag aagcgtaaaa
    11701 tcaatgagat cgtgtaactg gagctcgctg taactccact ttttgggtag tcggagctcg
    11761 ctgtagctcc gctttttttt cttgcggagg aacggtgtcc tccggctaca tagttttaga
    11821 aaggacttcg tcctctatct acagaagttc ttccagaaga tttttcagca gttccaattt
    11881 agtattcact tccgtaaatt tctctttttc tttggcgaca atttcttctt tggcattatt
    11941 caggaaattg ggattagcca gttttgcttg aattccgttt agttctttag ctaacttttc
    12001 gatttgtttg ctgaggcgat ttttttcact ttcaatatcc accaatccgc ttaaaggcaa
    12061 gaatatctct atattcctaa ctacagaagc aattgcagct ttaggttttt cgatatttat
    12121 acccccggat aatgttttta ccttagccag gcgattaaag taagccatat aattttcgaa
    12181 aagttcctgt tgtgattttt cagcaaagcg aataacaata tcaatttcct gagcgggagc
    12241 caagttaatt tgtttacgca agttccgaat ggcagttatg ctttcctgca cgaagctcat
    12301 atttctatca atatcattat ctataaggga ttcatctgcc tcagggaaag cggcaacaat
    12361 aagcgcttct tcttccatcg ggaagatctt tttaatgccc tgccaaattt cttctgtaat
    12421 aaaaggcata attggatgca gcaatcgcat tccattttgc attacattca gtaagatata
    12481 cttagctgtt aacttgcttt ccttttcggc agaaggattt aaacgatctt tactcagctc
    12541 aatataccag ctgcagaatt catcccacat aaatttgaag atagcgttgg cagcatcatt
    12601 aaaccttaaa ttttcatagt gttcgcgcac ttcgtttatt acttcctgca aacgagaaat
    12661 tatccatcta tcggcaagtt ccagttgtaa ttcattttct tggggcaatc cctcaatatt
    12721 ttccgcgttc atcataataa atctataggc attccacatc ttattggcaa agttgcgtcc
    12781 tgtttccaaa atgctttcac tgaaaacaac atctgcacct ttcggagtgc caaaaatcat
    12841 actgaacctt aaagcatcag cacccacgga attgattaaa tcaatcggat caggagaatt
    12901 acctaaggac ttactcattt tgcgtccgat gtcatctctc acggttccat gtagcaaaac
    12961 agtgtcaaag ggaattttat ccctaaaatg gagagtgctc ataatcattc tggcaaccca
    13021 caagtagata atttccggag ccgtaattaa cacattggta ggaagatagt atttaagatc
    13081 tgccgtttca tcaggccaac ccattgtaga aaatggccat aaccaactgg aaaaccaagt
    13141 atccaataca tcagaatctt gagtaaattt cgttcctcca cattgaggac acttttccgg
    13201 cattgctttt gctacaatca tattgttaca actatcacaa taatatacag gaatgcgatg
    13261 tccccaccaa atttgccttg aaatacacca gtcccgaata ttattcatcc agtgcatata
    13321 gacctttgtc cagcgttctg gctgaaactt tacttctcca ttttccacaa cttctatagc
    13381 ctttttagct aagggagcca ttttcacaaa ccactgatca gaaagatagg gttcaataac
    13441 cgtatcacag cgataacaac gacctacgga atagggatgc gtttccgtct tttccaaaag
    13501 attctgttcg gatagcatct gcaaaactgt ttcccggcag gcatagcggt ctaaaccttc
    13561 aaagttcttt cctgcatttg cattcataat tccggtttca tccattacca aaatttgggg
    13621 taaattgtgc ctgacgccaa tttcaaaatc gttgggatcg tgggcgggag ttacttttac
    13681 acagccagtt ccaaaatcct tatcaacata ttcatcagct ataataggta ttttcctatc
    13741 cgttaagggc aacaacagtt cttttccaag taaggataaa tagcgttcat cttttggatt
    13801 tacagcaacg gcaacatctc ctaacattgt ttccggacga gttgtagcca ctgttacata
    13861 accattccca tcagcaaaag gataatgaat ataccaaagg tgaccatttt catcagtatg
    13921 ctccacttcg tcatttgcca acgctgttac acaacgcgga caccagttga taatatattt
    13981 ccctttatat attaaacctt cttcatatag agttacaaaa acctctttca cagcccgcga
    14041 aagcatatca tccatcgtaa aacgtaaacg ttcccaatcg caacttgcgc ccaaaagttt
    14101 tagctgatct atgattatat tgcctttttc atttttccac tgccagattt tttccactaa
    14161 tttatcgcgg cctatatcct gacggcttat accctgttta gcaagatctt tttccaccat
    14221 attttgagtg gcaattccag catgatctac acccggcaac caaagcgttg gttctccttt
    14281 catccgatga taacgaacaa cgacatcttg aagagtatta tttagcacat gaccaatatg
    14341 taatattcct gtcacattag gagggggcat aagaactgta aatggctttt ttgttgaatt
    14401 ttcctgaggc ttaaaatagc cgttttcctc ccaaaattca taccatttct tttctagatc
    14461 tttgggctca aatgttttac tgatttccat tttagtagtt acctcgcttt attgtcgttg
    14521 tcgtttgctt tgcttaagca attattaatt gttttggaca atttctaaaa agagggttag
    14581 gcgtcaagga aaaggtttta ccgtttacgc agatttttta tcagatacaa ggattgtagg
    14641 aatagcatct tagaaacaag gattgtagga atagcatttt agaaacaagg atttaaggat
    14701 tttaggatta agaggattat tatatttata aatgattatt ataaagaggg ttataggaag
    14761 ctacttgggg cgggctttaa gtggttggct gtcggcttta agtcaaaatc ggccttttct
    14821 ataagattat tactgacctt attcaaagat aggggaaaag aatgaaatta
//
LOCUS       DAOSTH010000039        14785 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_4143_length_14785_cov_13.346911, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000039 DAOSTH010000000
VERSION     DAOSTH010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14785
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4143_length_14785_cov_13.346911"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            429..1466
                     /locus_tag="PLB65_05680"
     CDS             429..1466
                     /locus_tag="PLB65_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOQ78054.1"
                     /translation="MSIFDFFGMMANDIAIDLGTANTLVYKKNGGIVINEPSVVAVST
                     DNKKIIAIGNPAKLMLGKNPDEVRVIKPLKDGVIADFQVTELMLRNLILRAQKKRLLV
                     RPRVIVCVPSGITEVEKRAVRDSALHAGSREVHLISEPIAAAIGADLPIDMACGNMVM
                     DIGGGTSEIAVISLSHIVVHNSIRVGGDQIDMDIINYLRKKSNLLVGPQTAEKIKMTI
                     GSAYPLKQELSMDVRGRDIISGYPVTIKISSEEIREAISETVATIIDAIKRLFERTVA
                     ELSADIAERGIFLTGGGALLKGLDEKIRKTVDLPVHVVPDPTECVVKGAGKVLDDVDR
                     YRQVLIKRIED"
     gene            1525..2292
                     /gene="mreC"
                     /locus_tag="PLB65_05685"
     CDS             1525..2292
                     /gene="mreC"
                     /locus_tag="PLB65_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOQ78055.1"
                     /translation="MFKKNLVPLILIVLSLLMVIGSKESRIKKAGWMGNTVFFPFVKS
                     LKAIETTQELKQEVFSLRKQLAETTLEKLELQNQLKQFTGAYSISYDLGEVELIQAEV
                     IGLAGQYQELNLVVDKGFKFGIKINDPVISASGIVGKIIQVAADHSIVLPFSNPRFQL
                     PVMDARTSVQGILQSDISGTVSMNLIKLGSDIAVGDTIVTSNLSRLYPKSYPVGTIKR
                     IRESQDNLFISADINPFTLVENLEHIFILKRQEQIEL"
     gene            2282..2974
                     /locus_tag="PLB65_05690"
     CDS             2282..2974
                     /locus_tag="PLB65_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78056.1"
                     /translation="MSYSKKTEPVVTHWGVRFERIPVKTKILTPQDKISDIIKEYALP
                     LMKPGDILTISESPLAITQGRAIPVSEIRVSPLAKLLSRYVAKVPYGIGLGAPTSMQC
                     AIEETGIMRILFAAFIGAIGKLIGRKGDFYRVAGMQAALIDAATTSPVPPYTDTVIKG
                     PKNPQQICEDLHSEFGYEFAVMDINDIGGCWMIGGSKGVSKHFIESVMKDNPQGQGDE
                     LTPFCIVRRVDN"
     gene            2974..3468
                     /gene="mreD"
                     /locus_tag="PLB65_05695"
     CDS             2974..3468
                     /gene="mreD"
                     /locus_tag="PLB65_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOQ78057.1"
                     /translation="MVWKGIWSFILGLFFLYLQILVMPALALFKAIPFILLPWMIYIV
                     WTRPRNFALIVVFIIGMMFDTVNPMTFGFYSLLFCLLVLVIQEFRKPFEAESGVAKLL
                     AIGIANFIFSVLQLVMYGVTFGFDGELLAKSLVGFGYNVIVSIVVFWSMQLISKVRLT
                     INND"
     gene            3461..5374
                     /gene="mrdA"
                     /locus_tag="PLB65_05700"
     CDS             3461..5374
                     /gene="mrdA"
                     /locus_tag="PLB65_05700"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOQ78058.1"
                     /translation="MTKNQATITYSITLAVLFILLAASLFRLQVVEGAKYQRIAESNF
                     VRIRRIIATRGEIYDHKYRPIVQNVPSHNMYLTSGKINDINALSRFLEQNFGITESEL
                     KDLVLKQRFKTYEEILLADNVPYETVLALSEELNYYPELSFHIGSTRNYLYPNHFSGY
                     VGRINEKEYDLYKKEDYSLNSYIGKTGLERYYEVLLRGKDGREIVQVDAQGHSLQLFE
                     EGGNIEPLNGLSLVLTIDNDLQEFVSGIFPSGMKGAIVVSDVRSGGILAYVSKPDFDP
                     NLFMQKITPEIWEDLNRDEKPMVDRVIYGTYPPGSVFKPITAGIGLDRGLITPETHLA
                     FCSGGFKVGNRFFRCWFSSGHGSTTVVDALRVSCDTFFYDLSLKIKLDDFYNYVRDSH
                     LFGKTGIDLPNERQGFFPNTEWYKKTYGKNVGIIGHKVNLAIGQGEVLTTPLQINAFY
                     GALANNGLWIQPHLLKQTVGRGRLTREQVVPIAKRRLPMKPSTLKTIQQGLWAVVNAP
                     GGTGANAKINGATVYGKTGSAENVMGRKTHAWFCGYLVTSKPEIAVTVFLENAGGGGA
                     MAAPITAKIFNYYLGNLDIIKQVTPLPPQFRTAEGIENEEFEPGLEGSEVSIPGSEIE
                     PLPASLQEESDVH"
     gene            5364..6584
                     /gene="rodA"
                     /locus_tag="PLB65_05705"
     CDS             5364..6584
                     /gene="rodA"
                     /locus_tag="PLB65_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOQ78059.1"
                     /translation="MFINRKKFDFVLLTLLLLLIIFGCIVIFSASTTVIAGEAHTQNN
                     WWKQVIFAVLSLLAIILLLWIPMPIFDLVVLPLYLLNILALIIVLFTPIINGAHRWFS
                     VGGINLQPSESAKLLTILMVARVISKENLNEYRQILYGFLLTLIPVILIMIEPDFGTT
                     LVFWASLIAMLIAADVPLFYILLCISPIVSIISSIWWPAIPIWIIIFVLLLLKARLSW
                     VTITVASIINVFIALITPVFWMGLKDYQQNRILTFMDPTRDPLGAGYQIIQAKIAIGS
                     GSLFGKGWLKGTQKNMNFLPEHHTDFIFSVVGEEFGFLGSILLLILFAAFFSRIINNV
                     GELKIRERKVASAGILAYLMFQTFINIGMNIGLVPATGIPLPLISYGGSNLLINSIAV
                     GVVLKYMNERGFMK"
     gene            6581..7189
                     /locus_tag="PLB65_05710"
     CDS             6581..7189
                     /locus_tag="PLB65_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78060.1"
                     /translation="MKNRVLGLVLLALLSFFINACSVVTTLSFTERDIKDSPNLVENY
                     SFSPYSTIGEEALKGWMVILNPHDNSESPITIDPDTACEGKTSLRIDASDKSVLILSE
                     PFNVRRYGGYYLRCYFKTNSPNPPRPQLRFIVFKENGKIVNRFKTKAKINNDWQRTTI
                     SAGFIKPGARFGRLGIYIPPFKEGSIWIDDTSCFEVHGFKID"
     gene            7189..7626
                     /gene="dtd"
                     /locus_tag="PLB65_05715"
     CDS             7189..7626
                     /gene="dtd"
                     /locus_tag="PLB65_05715"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOQ78061.1"
                     /translation="MRITLQRVSKAVCYVDDKITGQIETGLLIFVGFSSFDTEAVLPL
                     AVRKCLELRIFEDENGKMNKSVSDINGSVLVISQFTLYANCQRGRRPDFTQAAPPGKA
                     KELYEEFVHLFKQTEINVQTGIFAAKMQIELVNEGPVTINLEF"
     gene            complement(7979..8266)
                     /locus_tag="PLB65_05720"
     CDS             complement(7979..8266)
                     /locus_tag="PLB65_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOQ78062.1"
                     /translation="MTKADLVKIISENTGIIRKDVAVVVDAFLQAIKDSLIKGNHIEI
                     RGFGTYKLKTRKPRVGRNPKTDEKVPVPERTVPTFKFSREFKNSVLDVKTK"
     gene            8800..9429
                     /locus_tag="PLB65_05725"
     CDS             8800..9429
                     /locus_tag="PLB65_05725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78063.1"
                     /translation="METAILSPLSNMCTFHCKGKLVFLSYGGREHKLFYGKGMQYLIT
                     LIANPDLYISCANIETIKPMPQRVYSQFSSSNEVQIHQLEVQSSFPSIPLTDLQTVKE
                     VKQELIRVIDTLAELEANCDYAQADDIRDQYEKLVQYLEQVYKPEGKIRYFPSEGKKT
                     KKRVLRAINRSLEDIEKLEPELAAELKNSLELGTILHYHPKIDIEVLGY"
     gene            9815..14605
                     /locus_tag="PLB65_05730"
     CDS             9815..14605
                     /locus_tag="PLB65_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOQ78064.1"
                     /translation="MKKNILILICLILISQFFAMAKQNEDRERPLYANNLVKIKLSSE
                     AVFRAQLPLSAYAECEKTNLNELDQLFEVNGVQSIIRAHISAKDRNWETKTGFDRWFL
                     LRLDGTKTVEQVIANLKSNRYIEDIIPEYYAYLHNVPDDPLYPNNWGHNNTAQLPVYQ
                     GDSHSGPGVGVIGFDSDAEIAWDSNQGYGDPDIIIGILDTGVDLNHPDLLLVSGYDFG
                     DNDDNPMDDSAEAGHGTCTSGIAAAKANNILGVAGIAGGCSVMPLKVASSNGDILYSA
                     ITNALIYAADNNVNVVSMSFGSNSHVGDNPTVDSALEYAYNNGVILFASTGNANSATI
                     GYPAIHPLVISVGAASPSGERKNPNSSDGENWWGSNYGTNQQDAAGAVDIMAPTILPA
                     TDITSFGGYSLDDYFMWFNGTSCSCPYAAGVAALVLSVNPTLTPAELRTIITSSATDM
                     TAGTSEGWDRWTGYGMVNVAQALCEANPSIPLCHIVYPLNNTGFEINSVITIQVEASD
                     PVERSILEVRFYLDDNPDYVFSDSEMPYEWVWNTAGLSIGQHTIKAEAIDNEGNRRYH
                     SISISLLNPADEGFETADFSKLIWLNKSSSPWTVQSDSYFTGEYSACSGLINAEGASE
                     LKLKVNITEAGEISFYRKVSSLANYGYLKFYIDDELIGEWSGEKDWACVSFPVNPGLR
                     TFSWAYQKNSSNVSGSDCCWIDHIIMPPYENYYWPPQNLTAAGGYGFVLLYWKVPEAG
                     EPTGYNILKNNVLLTTIAETRYTDTDVVAGISYEYTVVAVYAEGQSESTSAQTAIPTS
                     EVVTETIIGNGIIPTSNYEGCPINNYRKSLHGQMIYTARELNLHGIFGPTIITRLGFY
                     INTASAYSLPNFRIRMAHTTAEDVSQWLGEDDLITCYLATNYRPVAGDYDMLTFSTPF
                     LWNGIDNILVDTAFSLTVAVNSSGTVLYTDTPKGYHYTRSNVEDQTDVFTGGAVTMYK
                     PNMKLSVLTQPDIAVNTTNLSFGNVLAGYTAGKTFNITNTGTGYLNGEITVPAGYKIV
                     SLDEEHSSLSYSIRADSTAVFTVLFAPLAPGVYNGIISITHNGEGEERIINLSAVSLT
                     ALNTPFIEGFENYCENWTFVNEGQTNKWFYGFIVPQSGNGGLFVSGDNGLTNSYVPNR
                     GSTSYIYRDIYLPQGTENCKLRFSWKGMGEGTNPYFDFLRVFCVEPNFVPQAGTMITS
                     GQLGTTLNLSDHWKEADFNIPEPVSGVYKRIVFAWRNDASGETQPPAAIDNVRVILNS
                     NTDYAYVYDGSATVNLPAVSDTLGHTYHGNLIITGITEPDEYLVVTAGFASLNEPFSD
                     AGMDFTVTGANFSGAEITFNHNLGYVPYYLGYKIEGRGEWMTFSAAPNWTDKIAYFTV
                     PVYYGNATNISFCFPHANNVSPYYPELNVVLDMQNVVSIIWKCNYTEGISAYQIWRGS
                     SPSFNSAVAVSDLIPHLDTQEENYVYTDTTTDENTRYFYWLRIINIDESSSYWGPKLI
                     VTGTDPLQPPPETTLLGAYPNPFRYGTNISYTIKNAGNVEFKIYNLKGQLVNNYLQYH
                     NNYGTYIYEYTGTDKKGKELASGLYICYMKFGNKTFVQKIIKAN"
BASE COUNT         4647 a         2466 c         2958 g         4714 t
ORIGIN      
        1 agcttaacta aatcgattac tggcacttcc tgcttaggca attctaaaat gatcttctat
       61 catttactaa acgggattaa ggaagtggta gtgggatttt tttaagtaac ataaaagcta
      121 aaagcaaact aaaaaccccc gcacaaagaa ctcctgcact aagaactaag aacccctgca
      181 ctaagaaccc ccgcactaaa aacccctgca ctaagaactc ctgcactaag aactaaaaac
      241 ccccacacta atttcccaat ccatttttaa caaaactgga atctgggaaa tctgtaaaaa
      301 agatggaaaa aatggtaact gtcgttttta cttgacacag caggatatat aaaaaatagg
      361 gtataggtag aagaaatgtt aatttataaa ttcaagctgc taagcaagca ggaaatagag
      421 aggttttgat gtccatattt gacttttttg gaatgatggc caatgatatt gcaattgatt
      481 tgggaaccgc aaacacctta gtttataaga aaaatggcgg aattgtgata aatgagccct
      541 cagttgttgc tgtatcaaca gacaataaaa agattattgc tataggcaac ccagcaaaat
      601 taatgctggg taaaaatcct gatgaagtaa gagtgataaa acccttgaaa gacggcgtta
      661 ttgctgattt tcaggttact gaactaatgc tacgcaattt aattttacga gcccagaaga
      721 aacgtctttt agttcgtccc agagtaattg tttgtgttcc atctggaata actgaagtag
      781 aaaaaagagc tgtgcgtgat agtgctttgc atgcaggttc tcgtgaagtt catttaattt
      841 ctgaacccat tgctgctgcc ataggtgctg atttaccaat tgatatggct tgtggaaata
      901 tggttatgga tattggaggt ggaaccagcg aaattgctgt aatttcttta tctcacattg
      961 tagttcataa ttccattcgc gtaggtggtg atcaaattga tatggatata ataaactatt
     1021 tgcggaagaa aagcaacctg ttagttggtc cacaaactgc tgaaaaaata aaaatgacga
     1081 ttggcagtgc ttatccttta aaacaagaat tatctatgga tgtgcgtgga cgagatatca
     1141 tatctggtta tcctgtaaca ataaaaataa gctctgaaga aattagagaa gcaatttcgg
     1201 aaacagttgc gaccatcatt gatgccataa agcgactttt tgaaagaaca gtggcagaac
     1261 tttctgctga tattgctgaa cggggtatat ttttaacagg tggaggcgct ctccttaaag
     1321 gcttggatga aaaaattcgt aaaacagtag atctacctgt tcatgttgtt cctgatccta
     1381 cagaatgcgt agttaaaggt gctgggaaag ttttggatga tgtagaccgt taccgtcagg
     1441 ttctaataaa aagaattgag gactgatcta aaatctgaac ctattaccca agatgataac
     1501 tccctatttt aaaagggacg aaatatgttt aagaagaacc ttgttcccct tattttaatt
     1561 gtcctttctc tactaatggt gataggcagt aaggaaagtc gtatcaaaaa agcaggctgg
     1621 atgggaaata cggttttctt ccctttcgtt aaatctctaa aagcaattga gaccacgcaa
     1681 gaactgaagc aggaagtttt ttctttgcga aaacaattgg ctgaaacaac tttggaaaaa
     1741 ctggaactac aaaaccagct gaaacagttt actggagctt attctatttc ctatgatctt
     1801 ggtgaagtag aattaattca agcagaagtt attggtttag ccggtcaata tcaggaactt
     1861 aatttagtgg tggataaagg attcaaattc ggaatcaaga ttaatgatcc ggttatttca
     1921 gctagtggga ttgtagggaa aataattcaa gtggcagccg atcattctat tgtgcttcct
     1981 ttttccaatc cgcgttttca attgccggtt atggatgcgc gcacatctgt tcaggggatt
     2041 ttacaatccg atattagtgg aacggtttca atgaatttaa ttaaattggg ttcagatata
     2101 gcggtaggag atacaattgt aacttccaat ctttcacggc tttatccaaa atcctatcct
     2161 gtaggaacca taaaaaggat tagagaatct caggataatt tgtttatcag tgcagatata
     2221 aatcctttca ctttagtgga aaatcttgag catatattta ttctaaaaag gcaggaacaa
     2281 attgagctat agtaaaaaaa cagaaccggt cgtcacccat tggggagtgc gatttgaacg
     2341 cattccggtg aaaacgaaaa tccttacccc ccaagataag atcagtgata taattaaaga
     2401 atacgcttta ccattaatga aaccaggtga tattctaaca attagtgaaa gtcctttagc
     2461 catcactcag ggaagggcaa ttcctgtttc tgaaataaga gtttctcctt tggcaaaact
     2521 acttagtcgc tatgtagcta aagtcccata cggtattggt ttaggcgctc caaccagtat
     2581 gcaatgcgct attgaagaaa cagggattat gcgtattctt tttgcggctt tcattggtgc
     2641 cataggaaaa cttataggca ggaaaggtga tttttataga gtggcaggaa tgcaagctgc
     2701 tttaattgat gcagctacaa cttcacctgt tcctccttat acagatacag taattaaggg
     2761 accaaaaaat cctcaacaga tatgtgagga tttacactca gaatttggct atgaatttgc
     2821 cgttatggat attaatgata ttggtggttg ttggatgatt ggggggagca agggtgttag
     2881 taaacatttc atagaaagcg tgatgaaaga taatcctcag gggcagggtg acgaattaac
     2941 tccattttgc attgttcgaa gagtagataa ctaatggttt ggaaaggaat ttggagtttt
     3001 attcttggcc tgttttttct gtatcttcaa attctggtta tgcctgcttt agcattattt
     3061 aaagcgattc ctttcattct tttaccttgg atgatttata ttgtttggac tcgtccgcgt
     3121 aattttgcac taattgttgt ttttatcatc ggaatgatgt ttgatacagt aaatccgatg
     3181 acttttggct tttattctct tcttttttgt ttgctggttt tagtaatcca ggaatttcgt
     3241 aagccctttg aggcagaaag cggtgtagcc aaattgttgg caattggaat tgctaatttc
     3301 atcttttctg ttttgcaact ggttatgtat ggagttacat ttggttttga tggggaactt
     3361 ttggctaaat cattagtcgg ttttggctat aatgttatag tatcgattgt cgttttttgg
     3421 agtatgcaac taattagtaa agtaagatta acgattaaca atgactaaaa atcaagctac
     3481 cataacctat tcgatcactt tggcggttct gtttatttta cttgctgctt cgctttttcg
     3541 tttacaggta gtggaaggag ctaaatatca acgcatagcg gaaagtaact ttgtgcgcat
     3601 tcggagaata atagccacgc gcggagaaat ttatgatcat aaatacaggc caattgttca
     3661 aaatgtccct tcgcataata tgtatttaac cagcgggaaa attaatgata taaatgctct
     3721 aagcagattt ctggaacaga atttcggcat tacggaaagc gaattgaaag acctggtatt
     3781 gaaacaacgt tttaaaactt atgaagaaat tctgctggca gataatgttc cctacgaaac
     3841 agtattagct cttagcgaag aattgaatta ttatcctgag ctttctttcc atatcggttc
     3901 cacccgtaat tatctatatc caaatcattt tagcggttat gtagggagaa ttaacgaaaa
     3961 agaatatgac ctgtataaaa aagaggatta ttctctgaat agctatatcg ggaaaacggg
     4021 tttggaacgc tattatgaag ttttactacg cggaaaagat ggccgagaaa ttgttcaggt
     4081 ggatgctcaa gggcacagtc tacaactttt tgaagaaggc ggaaatattg aaccgcttaa
     4141 tggactttcc cttgttctta ctatagataa tgatctgcag gaatttgtct ctggaatttt
     4201 tccctctgga atgaaaggag ctatagtagt tagcgatgtt cgcagtggag gcattcttgc
     4261 ttatgtaagt aagccagatt ttgatcctaa tctctttatg caaaaaataa caccagaaat
     4321 atgggaagat ctgaatcgcg atgaaaagcc aatggtagat agagttatct acggaaccta
     4381 tcctcccggt tcagttttta aacctattac tgcaggtatt ggtttagacc gcggtttaat
     4441 tactcctgaa acacacctgg ctttttgttc gggaggtttt aaagttggta atcgtttttt
     4501 ccgatgctgg tttagcagtg gacatggttc tactacagtt gttgatgctt taagagtttc
     4561 ttgtgatact ttcttttatg atctttcctt aaagataaaa ttggatgact tttataatta
     4621 tgtacgggat tcacatctat ttggaaaaac gggaattgac ctaccaaacg aaagacaggg
     4681 cttctttcct aatacagaat ggtataaaaa aacctacggc aaaaatgtag gaattattgg
     4741 acacaaagta aatctggcta ttggacaagg agaagtatta actaccccct tacagataaa
     4801 tgccttttat ggagctcttg ccaataatgg tttatggatt caacctcatt tattgaaaca
     4861 aacagttggt agaggacgtc ttactcgtga acaggttgta cctatagcta aaagaagatt
     4921 acctatgaaa ccaagtaccc taaaaactat tcaacaggga ttatgggctg tagttaatgc
     4981 tccaggtgga acaggagcaa atgcaaaaat aaacggagct actgtttatg gtaaaaccgg
     5041 ttctgccgaa aatgttatgg gtagaaaaac tcatgcctgg ttctgcggtt atttagttac
     5101 ttccaaacca gaaattgcag taactgtttt tctggaaaat gctggagggg gaggagctat
     5161 ggctgctcct ataactgcta aaatattcaa ttattatttg ggtaatttag atattattaa
     5221 acaggtaact cccttgccac cacagtttag aacagcggaa ggcattgaga atgaagaatt
     5281 tgaacctggt ttagagggct cggaagtatc tatccctgga agtgaaattg aacctctacc
     5341 ggcttcattg caggaagaaa gtgatgttca ttaaccgaaa aaagttcgat tttgtcctct
     5401 tgacgctttt attgctgctg attatttttg gctgtattgt aattttcagt gcttccacaa
     5461 ctgtgattgc gggagaggca cacactcaaa ataattggtg gaagcaggtt atctttgctg
     5521 tcctttcctt attggccata attctattgt tatggattcc aatgccgatt tttgatttag
     5581 tagtattacc cctttacctt ttgaatatac tggctttgat catagtgtta tttactccca
     5641 taattaatgg agctcatcgt tggttttctg taggaggaat aaatctccag ccctcggaaa
     5701 gtgctaaatt gctaacaatt ttgatggtag cgcgggttat atctaaagaa aatttgaatg
     5761 aatatcggca aattctctat gggtttttat taaccctaat acctgtaatc ttgattatga
     5821 ttgaaccaga ttttggtaca acgctggttt tttgggcttc tctgatcgct atgttgattg
     5881 ctgctgatgt acctttattt tatattttgc tatgcataag tcctatagta agtataataa
     5941 gttccatctg gtggccagct attccgatct ggattataat ttttgtatta ttactattga
     6001 aagcgcgtct ttcttgggtt acaattacgg tggcaagtat tatcaatgta tttatagcat
     6061 taattactcc tgttttttgg atgggattaa aggactacca acaaaatcgg attttaactt
     6121 ttatggatcc tactcgtgat cctttgggtg caggttacca aattattcaa gctaaaattg
     6181 ctatcggtag cggttctctg tttggcaagg gctggctgaa aggaactcaa aaaaatatga
     6241 atttcttgcc ggaacaccat acggatttta ttttttctgt agtgggtgaa gaattcggtt
     6301 ttttgggctc tattcttcta ttaattttgt ttgcggcatt cttcagcaga attattaata
     6361 atgtaggtga actaaaaatt cgcgaaagaa aagttgcttc agcaggcatt ttggcttatc
     6421 ttatgtttca gacctttata aatattggaa tgaatattgg tttagtccca gcaacgggaa
     6481 ttccgttacc cttaattagt tacggtggtt cgaatctatt gattaatagc atcgccgtag
     6541 gagttgttct aaaatatatg aacgaaagag gttttatgaa atgaagaatc gcgtgcttgg
     6601 tcttgtctta ttggcattac tatcgttttt tataaatgcc tgttcagttg taaccaccct
     6661 cagttttaca gaacgagata ttaaagatag ccccaatctg gtagaaaatt atagttttag
     6721 tccctattcc acgattggag aagaggcttt aaagggttgg atggtaattc ttaatcctca
     6781 tgataattca gaaagcccta ttactattga tccagatact gcttgtgaag ggaaaacttc
     6841 tttacgaata gatgcttctg ataaatctgt gcttatcttg agtgagccat ttaatgtccg
     6901 acgctatgga ggatattatt taagatgcta ttttaaaaca aattctccta atccaccccg
     6961 tcctcaacta aggtttattg tctttaagga aaacggtaaa attgttaatc gcttcaaaac
     7021 aaaagccaaa attaataacg attggcaaag aacaacgatt agcgctggtt tcattaaacc
     7081 aggtgccaga tttggacgcc tgggtattta tattccacct tttaaagagg gctctatctg
     7141 gatagatgat accagctgct ttgaagtgca tggattcaaa atcgactgat gcgtattact
     7201 ttgcaaaggg ttagcaaagc agtttgctat gtggatgata aaataaccgg acaaattgaa
     7261 accggacttc ttatttttgt ggggttcagc tcttttgata ctgaagcagt tttaccttta
     7321 gcggttcgta aatgtctgga actgcgtatc tttgaagacg aaaatggcaa aatgaataaa
     7381 agtgtctccg atatcaatgg ttcggtgtta gttatctctc aatttaccct ctatgccaat
     7441 tgtcaaagag gaagaagacc agattttact caagctgcgc ctccgggaaa agcaaaagag
     7501 ctttatgagg aatttgttca tctatttaaa cagacggaaa tcaatgtcca aacaggtatc
     7561 tttgctgcca aaatgcaaat agaactcgta aatgaaggcc cagttacaat caacttagag
     7621 ttttgatgtt taatttaaaa aagagagatt ctagtagaac acaattaaat tgattttaat
     7681 gaattaacac agcgatgtag gcaagcggca ctgaggatag cgttttttcg ttatagtgat
     7741 agggaacaaa aaaacactat gaaaatatct gtctgtatgt ttctcataat agaaaaaaat
     7801 cgccaaaata aaaaaagccg tttctcccca aatgaaacgg ctataaactg atcaaaagat
     7861 agctttttta tctatcattt ccgtttatga cgattcttac gcagacgctt cttcctctta
     7921 tgggtagcaa ttttatgacg ttttctcttt tttccgcaag gcataatctt ctctcttatt
     7981 actttgtttt tacatcaagg acagaatttt tgaattcgcg ggagaattta aaagtaggaa
     8041 cggttctttc aggaaccgga actttttcgt cagtcttggg gttgcgtcca acacgaggtt
     8101 tacgggtttt gagtttatat gttccaaaac cccggatttc gatatgatta cctttaatta
     8161 agctatcttt aatggcttgt aaaaaggcat caacaacaac agcaacatct ttacggataa
     8221 ttccggtgtt ttccgaaata atttttacta aatcggcttt tgtcattttt ccatctcctt
     8281 aaatatactt atcgtcaata ttattcattg ctatatcgca ataaggtatt catagataaa
     8341 accttatgat ttatgtcaag agaaatatct aaaaatatgc aatgagtagt gctgatattc
     8401 cttttacaat aaagcattaa taggttttaa tgaaatcaca ttttttgtgt tcccctttgg
     8461 taaattatcc tttaaacacg ctaaaccaaa tagcaaatta aggattatag gatttttcga
     8521 acagacccag aactatcgca gaatgaattg gttacatcat tgaattaggc atcatccctt
     8581 aaccccaatt ttattaacaa agaaagggac aaaatatgaa ataaggtgtg gcagaaacag
     8641 atagaattac cacatcgcaa gttaaggaaa tgggtacgag tagagaataa ttagcaacac
     8701 taatatttct cttgacatat taagcggtac tgaaattatg aagctggatt gtgaattatg
     8761 aaaaaataac ttgatcaata aataaaaagt aggtgagata tggaaacagc cattttaagt
     8821 cccctctcta atatgtgtac cttccactgt aagggaaaat tagtttttct ctcctacggt
     8881 ggaagagagc ataagctttt ctatggtaaa ggaatgcagt atcttataac tttaattgcg
     8941 aatccggatt tgtatatttc ttgtgctaat attgaaacta taaaaccaat gcctcaaagg
     9001 gtatacagtc agttttcctc atctaatgaa gtgcaaattc atcaattgga ggttcagagt
     9061 agttttccct ctattccttt aaccgattta caaacggtta aagaagtaaa gcaagaatta
     9121 attagagtga tagacacact tgctgaattg gaggcaaatt gtgattatgc acaagctgat
     9181 gatatcagag atcaatatga gaaacttgtt caatatttgg aacaagttta taagcccgaa
     9241 ggtaaaattc gttattttcc cagcgaaggg aaaaaaacaa aaaagagagt tttacgtgct
     9301 ataaaccgtt ccttggaaga tatagaaaaa ttggaaccag agcttgctgc agagctaaag
     9361 aactccttgg aattgggaac tattttacat tatcatccta aaatagatat agaggtttta
     9421 ggatattaaa caatagttct tgttttcaag ctttattact tatttaaaat gtgctgcaca
     9481 taaaagggtt gtgtctcaag gttggtgtaa aattggggaa gccagattaa gaaaaaagta
     9541 gagccggatt ccgctctttg ttttgatggt tttgctaaaa ctaaaaagac aaggataaga
     9601 tcataaccca acccaagaga aagaaggatg aagatagtat aattgcaagt atatgctaac
     9661 atagattgtg agaaatgaga gtagtaaaat gttttccaac aacccgatat atagcgagtt
     9721 ggaatttaca ccaacattgg ggactcaact attgtgagca tagcattaaa gcaaagttct
     9781 tatctggaat gaatattgct tttaaaattg aattatgaaa aaaaatatct taattcttat
     9841 ctgtcttata ctgatcagcc agttttttgc aatggcaaag caaaatgagg atagagagcg
     9901 tcctctttat gctaataacc ttgtaaagat taagctaagt tcagaagcag tatttcgtgc
     9961 ccagttgccg ttatcagctt atgctgaatg tgaaaaaaca aacctcaatg aattagatca
    10021 gctttttgag gtgaatggcg ttcaatcaat tatccgcgct catatttctg ccaaagatag
    10081 aaattgggaa actaaaacgg gatttgaccg ctggttttta ctgcgtttag atggcacaaa
    10141 aacagttgaa caagtaatag ctaatctaaa aagcaatcgc tatatagaag atattattcc
    10201 cgaatattat gcctatctgc ataatgttcc cgatgatcct ctatatccca ataattgggg
    10261 acacaataac actgctcagc tacctgttta tcaaggtgat tctcatagtg ggcctggagt
    10321 aggggttata ggatttgata gcgatgcaga aattgcctgg gatagtaatc aaggttatgg
    10381 agatcctgat attattattg ggattcttga tacaggtgta gatctaaatc atccagattt
    10441 acttttagtt tccggatatg atttcggcga taatgatgat aatccaatgg atgatagtgc
    10501 cgaagcaggt catggaacat gcacttctgg aattgcagca gctaaagcaa ataacatttt
    10561 aggggtggca ggaattgcag gtggctgtag tgttatgccg ctaaaagtag ccagcagtaa
    10621 tggagatata ctatattcag ctattaccaa tgctcttatt tatgcagcag ataataatgt
    10681 aaatgtagtc agtatgagtt ttggttcaaa ttctcatgta ggggataatc ctactgttga
    10741 ttcagcttta gaatatgctt ataataatgg agttattctc tttgccagta ccggaaatgc
    10801 aaatagcgct acaataggtt atccagctat acatccttta gtaatttcag ttggcgcagc
    10861 aagtccttct ggagaaagaa aaaatcccaa ttcctcagat ggagaaaatt ggtggggttc
    10921 aaattatgga accaatcaac aagatgctgc aggtgcagtt gatataatgg cacctacaat
    10981 tcttcctgca acagatatta cttcctttgg aggttattct ctagatgact attttatgtg
    11041 gtttaatgga acatcctgtt cttgtcctta tgcagcagga gtagcagctt tagttctttc
    11101 tgtaaatcca actcttactc cggcagaact tagaaccata ataacttctt cagccacaga
    11161 tatgacagca ggaacatctg aaggttggga tcgatggact ggttatggaa tggtaaatgt
    11221 tgctcaggca ctttgtgaag ccaatccaag tattcctctc tgccatattg tttacccgct
    11281 taataatact ggttttgaaa tcaattctgt tattactatt caggttgaag catctgatcc
    11341 ggttgagcgc agtattctgg aagttcgttt ttatcttgat gacaatccag attatgtttt
    11401 ttctgattca gaaatgcctt atgaatgggt atggaataca gcaggtttat ctatagggca
    11461 gcatacaata aaagctgaag ctattgataa tgaaggaaat cggcgatatc attctatatc
    11521 catatcactc ttaaatcctg ctgatgaagg attcgaaaca gccgattttt ccaagttgat
    11581 ttggcttaac aaaagttctt cgccttggac tgtgcagagc gattcatatt ttactggaga
    11641 atattcagct tgttctggtc taataaatgc agaaggagct tcagagctga aattgaaagt
    11701 gaacataacc gaagctggag aaatcagctt ttaccggaag gtttcttctt tggcaaatta
    11761 cggttatttg aaattctata ttgatgatga attaattgga gaatggagtg gagaaaagga
    11821 ctgggcttgt gtttcgtttc ctgtaaatcc cggtctacga acattttcct gggcgtatca
    11881 aaaaaattcc agtaatgtat cggggagtga ttgttgttgg atagatcata taattatgcc
    11941 cccctatgaa aattactatt ggcctcctca aaatctaact gctgcaggcg gatatggatt
    12001 tgtgttacta tattggaaag tacctgaagc gggtgaacct actggctata atattttaaa
    12061 gaataatgtg ttgcttacta ccattgcaga aaccagatat acggatactg atgttgtagc
    12121 tggcatttca tatgaatata cagttgtggc agtttatgcc gaaggtcaat ctgaatcaac
    12181 atctgctcaa actgctattc caacttctga agtagttacc gaaaccatta tcggaaacgg
    12241 aatcatccca acttctaact atgaaggatg tcctatcaat aattatcgta aaagtttaca
    12301 cggtcagatg atctataccg ctcgggagct taatctacac ggtattttcg gtcctacaat
    12361 tatcactcgt ttaggatttt atataaacac agcttcagca tattcacttc ctaatttcag
    12421 aattcggatg gcacatacta ccgcagaaga tgtaagccaa tggcttggcg aagatgatct
    12481 tataacttgt tatctggcaa ctaattatcg accggtagct ggtgattatg atatgctcac
    12541 tttctcaact ccctttttat ggaatgggat agataatata ttagtggata ctgctttcag
    12601 tttaactgtg gctgtaaatt cctctggaac agtactttat actgatacac ctaaaggcta
    12661 tcattacaca cgcagtaatg tagaggatca aactgatgtt tttactggag gtgctgtaac
    12721 tatgtataaa ccaaatatga aattatcagt tcttactcaa ccagatattg cagtgaacac
    12781 caccaatctt tcttttggaa atgtgcttgc tggttataca gcaggtaaaa catttaatat
    12841 aactaatacc ggaacaggtt atttaaatgg tgaaattaca gttcctgcag gttataaaat
    12901 tgtatccttg gatgaagagc atagttcttt atcatattct attagagctg atagcacggc
    12961 tgtgtttact gtcctttttg cacctttagc accgggtgtt tataatggaa ttatatctat
    13021 tacccacaat ggggaagggg aagagagaat tataaactta agtgcagttt ctcttactgc
    13081 gctaaatact ccttttattg agggcttcga aaattattgt gaaaattgga cttttgtaaa
    13141 cgaaggtcaa acgaataaat ggttttatgg gtttattgtt cctcaatcgg gaaatggagg
    13201 actatttgta agtggtgata atggactcac taattcttat gttcctaata gaggctctac
    13261 aagttatata tacagagata tttatcttcc ccaaggaacc gagaattgta aattgcgttt
    13321 ttcctggaaa ggaatggggg aaggaacaaa tccatatttt gattttctca gggttttttg
    13381 tgtagaaccc aatttcgtac ctcaagcagg aactatgata accagtggac agcttggcac
    13441 aaccttaaat ctttccgatc attggaaaga agccgatttt aatattcctg aaccggtttc
    13501 tggtgtatat aaaagaatcg tttttgcctg gagaaatgat gcttcgggtg aaacgcaacc
    13561 tcctgctgca attgataatg ttcgcgttat attaaatagc aataccgatt atgcttatgt
    13621 ttatgatgga tcagccactg taaatttacc tgcggtttcg gacaccttag gccataccta
    13681 tcatggaaat ctaattatta ctggaattac agaacctgat gaatatttgg tagttacggc
    13741 tggctttgct tctttgaatg aacctttttc tgatgccgga atggatttta ctgttacagg
    13801 agccaatttt agtggagccg aaattacttt taaccataat ttaggttatg ttccttatta
    13861 ccttggttat aaaattgaag gaagaggaga gtggatgacc ttttccgctg ctccaaattg
    13921 gacagataaa attgcttact ttacagttcc cgtctactat ggaaatgcca cgaatatatc
    13981 tttctgcttt cctcatgcga ataatgtatc tccatactat ccagaattga atgtagtgct
    14041 cgatatgcaa aatgtagtta gcataatatg gaaatgtaac tatacagaag gaatttctgc
    14101 ttatcaaata tggaggggaa gcagcccttc tttcaattca gcagttgcag taagtgatct
    14161 aatccctcat cttgataccc aagaagaaaa ttatgtctat accgatacta caactgatga
    14221 aaatacgcgt tatttttact ggctaagaat tatcaatata gatgagtctt ctagttattg
    14281 gggaccaaaa ctaattgtta ctggaacaga tccattacaa ccacctccgg aaactactct
    14341 tttgggagcg taccctaatc catttagata tggaaccaat atcagctaca ctatcaaaaa
    14401 tgctggaaat gtggaattta aaatctataa ccttaaaggt caattggtaa acaactatct
    14461 ccaataccat aataattatg gaacttacat ttatgaatat accggtacag acaaaaaagg
    14521 caaggaacta gccagtggac tatatatctg ctatatgaaa tttgggaata agacctttgt
    14581 tcagaaaata ataaaggcta attaagttaa ctgcaaaaaa tataaacggt tttatcgata
    14641 taaaagttga agcgttaaat ggttgtttaa cctagtgatc gctgttgtgt aaatcagaag
    14701 ttcaaagttc agggtactgg agttcaaatc tgtcgttcct ccgaaaaaaa tattctctca
    14761 cagattacac agattacaca gatat
//
LOCUS       DAOSTH010000040        14424 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_4270_length_14424_cov_16.621268, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000040 DAOSTH010000000
VERSION     DAOSTH010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 14424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14424
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4270_length_14424_cov_16.621268"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..520
                     /gene="rpmE"
                     /locus_tag="PLB65_05735"
     CDS             311..520
                     /gene="rpmE"
                     /locus_tag="PLB65_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HOQ78065.1"
                     /translation="MKKAIHPKYEMVTVTCACGNTFQTKSTKDKLQVEICNACHPFYT
                     GKQKIIDTAGRVEKFNKKYNLKQEN"
     gene            601..1575
                     /locus_tag="PLB65_05740"
     CDS             601..1575
                     /locus_tag="PLB65_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HOQ78066.1"
                     /translation="MSEKKEISVGGQAVIEGVMMRGPDLLATAIRRKDGSIELLKTPF
                     VSITQKKKFLGLPIIRGFISLIEMMIIGIKTLTFSANRFELDFIAEEKAKGKKVKSKN
                     KTAEQAENIVSYIIAFGLAFLLFGWLPYKLADWVHLSRQDFLFNVFAGVIRIIFFVLY
                     VYLISLMKDIKRLFRYHGAEHKNVNAYEHNSPLIIDKIKEWSTIHPRCGTSFIFFVLL
                     ISILIFSIVDTLVSAFILHSPVPVYLRLAYHILLMPLISGVSYEVLKFSGKNLKHPIV
                     KLLTVPGMALQHITTQPPDDEMLEIGLVAMKAALDMDISDHNVKIVEE"
     gene            1581..2648
                     /gene="prfA"
                     /locus_tag="PLB65_05745"
     CDS             1581..2648
                     /gene="prfA"
                     /locus_tag="PLB65_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOQ78067.1"
                     /translation="MLPLEKLQSLENELNEMQEQISDPALINDTKKYRDCLRRFKELK
                     EIDSAWKHYRKLEKHMEEVKELEKSENDPEMLNLIKDEENSLQNQLEQAELKLRDLLI
                     PSDPNDSKNAIVEIRAGTGGEEAALFAADLFRMYSHYAEMKGWKLEVISMNETELGGY
                     KEVVFQLSGKDVYSYMRFESGVHRVQRIPVTESSGRIHTSAVTVAVLPEAEDIDIDIQ
                     DSDLRIDVYRSTGHGGQSVNTTDSAVRITHLPTGIVVTCQDEKSQIKNKAKALKVLRS
                     RLLDAEISRQEQEIARARKAQVSTGDRSAKIRTYNFPQSRVTDHRINLTSYNLDSFLA
                     GNIEEFIQALRIAWRNEKVNT"
     gene            2649..3902
                     /locus_tag="PLB65_05750"
     CDS             2649..3902
                     /locus_tag="PLB65_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOQ78068.1"
                     /translation="MAVAQIVLILIVLLFFLVLSFFFSGLETGMLSIDQIKLEQEAKK
                     SKSKAVLLQFVRQPDKFLGTTLIGNNIANVIIASLSTYLANRLFAPVFDARYTSLVVG
                     IVVLISGEMIPKTIFRDNAETLIPLFFPLLQFFYYLLKPLVMIVTWLNNFLRKLLKLE
                     EGYQYDYLTKDDLSFLLSQTAIDETNDHQIEMIEDALDFSELDARNVMVPRTDIVAIQ
                     DKTSLSEVANIARKEGFTRYPVYCNNIDDIIGILIIYDILKQDCSPDMTAGDIIHEPL
                     FAPENTDLDVLLKEMQTKQRSMAIIVDSFGGTAGIVTMEDILEEIVGDIEDEYDTDED
                     ENSVQQVGPKTWLVAADTEIDRLIDDYGIQLPEGDYETVAGLILDRLEKIPHQGQVVN
                     VPPYTIQVLQATSKQIVKVKIHKIN"
     gene            3917..5602
                     /gene="ftcD"
                     /locus_tag="PLB65_05755"
     CDS             3917..5602
                     /gene="ftcD"
                     /locus_tag="PLB65_05755"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HOQ78069.1"
                     /translation="MKLMECVPNFSEGRDSSILNAIAAAIKSVKGVVLLDMDPGADTN
                     RTVFTIAGEPEAVVEAAFQAIKKAAELIDMSKHKGAHPRMGATDVCPFIPISEMTMEE
                     CVEYANKLGKRVGEELGIPVYLYEYAATKEEWKNLANIRSGEYEALSEKAKDPYWKPD
                     FGPHTFNAKSGATAIGAREFLIAYNINLNTRDKKKASEIAQIIRESGRAARDKNGKLM
                     KDENGNTIYIPGLFTHCKAVGWFIESYDRAQISINLTNYKITPPHLVLEKVRELASEM
                     GIQVTGSELVGLIPKAAILEAGKFYLSRMNESTGIPERMIMQTAIQSMGLAELAPFDI
                     DKKVIEYAISRKDRLVNLKVDAFCDLLSTDEPAPGGGSVAALCASMSGALSAMVANLT
                     IGKKGYENVAKEAMELAPIGQEIKEKSLEVIDKDTDAFFTMMDAMRLPKKTEEEIEFR
                     KTQIEKCTQAAINAPLETMHLAMSAAELALKIAKIGNANALSDAGVAALTANCASQAA
                     YYNILINLPGIADENYIHKIHAEAKDLLEKCNLLCDSVEKDVLAKLMGEKVER"
     assembly_gap    5611..5710
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6108..6779)
                     /locus_tag="PLB65_05760"
     CDS             complement(6108..6779)
                     /locus_tag="PLB65_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HOQ78070.1"
                     /translation="METIQTHPYKPFLPEEAKVLLLGSAPPYRFCTGYINDLKNKDID
                     YYYGSNSNLLWDILFLALKPESINCISELRFRQDERIKRTAYQVNFLQSFLAENKLAM
                     ADILLKFIRKDTSAEDYKLKVLKYQDILTILYSRPTLNKILCTSRNRVHYWLVDYLQQ
                     KEKLDLVKMDENQTKLILKSGVQREIKIDILPSPSARSVMRYDNKEDFLQKVSEIYKQ
                     RILNN"
     gene            complement(6794..7714)
                     /locus_tag="PLB65_05765"
     CDS             complement(6794..7714)
                     /locus_tag="PLB65_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78071.1"
                     /translation="MLKRFFILLSLLIAGYLYANYTLQLKTNNLVIYAESPISVPLET
                     MLKSLDDNIDSFQMNIGVYLDLLAPVYLIENNTSYQTLSLGKAKIVEFSDAFYSGSEQ
                     RIYVRPLASIQENHRKVLMHEYIHWYLEQIFEQAPLWFHEGMATHYAQQVGFEQFIYY
                     LEQSFLGKKSDLFRLSYSYPEKKEDWSLFYLSSAMAVRYMSEKKPKEWQDFWELAASY
                     QRKKTKASFTDCFVRAYRTTLYDFHKEYSQYTKRLSYQYLFWSFNALLAILLPIILFI
                     AWRIRKKRMAALPDLPLPEDEIINEDETID"
     gene            complement(7808..8068)
                     /locus_tag="PLB65_05770"
     CDS             complement(7808..8068)
                     /locus_tag="PLB65_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOQ78072.1"
                     /translation="MRLDLLLNKLCLTKTRSIAKNACDKNLVLVNGKPAKASTIIKAG
                     DIIIFRLYNAEHEIRITQIPEGNVAKKDSTKYYELLRKEDLS"
     gene            complement(8043..10958)
                     /locus_tag="PLB65_05775"
     CDS             complement(8043..10958)
                     /locus_tag="PLB65_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOQ78073.1"
                     /translation="MNKMNQSFYRMLFDKSPVSLWIEDFTGIQAELEKMKKRGISDLR
                     THLKLHPDQLDALVNKLVVVDVNNATLSLYKAKDKQELLNNLQLVFKGEVKEYLLESF
                     IAVDEGKEYFEGQGINYDLEGNKLYFKITWSIPESEQKDYETVIVAMQDMTKLTLTSN
                     ELEKREALFRCIFEQSSEGMLLLDNSGTIMMANETMGEHLGIPINRIIGSYLWDVYEE
                     FGKKVTYETSYELSKEKVLRVISEHQDRPREAQFSFLDSRHKVYARKHTLMPISTKDV
                     TLYAFISTDLTPAYRSERITAILHKISHAVNTTESLDELFHIIHDSLATIIDATNFYI
                     ALYDGKNNMITFPYLVDEMEENASPVVADDSKSLTAQIINEARPLLLDADEIQDRVKI
                     IGAYGVECKNFLGIPLILSGNVIGALVVQSYTKTNLYDEEDQLLLESVSEQIAFALHK
                     KQTDDSIKILIQAIEQAGEGTIIFSPEGNIKYVNSIFEKIIGFRKIDLIDKPFEFLPF
                     ESSKRWNMKQSWMRVRSSQPWRGKVDLTRKDGSKATLDMIVKPVIDDEGRLSSIIASC
                     KDVTYEILQEEQMKRSQRLEALGRLTGGIAHDFNNILSAIVGYTELAADDLIPESDAA
                     MNLLEVLKSATRAKEMIKQLISFSRQEESKTEVIDLVDHVRESVRFLKSYMPRSIQIK
                     EDYKTDKSSVLAVPSQIHQIVINLGTNSMQALKRDDALIEISVENVSFRSSDMQAFPE
                     LEQSQYLKISVSDNGTGIEPSIIDHIFDPYFTTKGATDGTGLGLSIVHSIIQAHHGAI
                     RVKSEPGFGTTFDVYLPLYTSEQRREDSTEEIVDEIDYSGTECIMFVDDEPSLVSVFR
                     QGMMRLGYKVEGFTDPRKAEEFFSKNPDAVDIVVTDTTMPYINGVDLAARLMTIKPDI
                     PIIICTGFTTLISMEEARQRGIRDYIMKPFKIKDIAIRIKDILNSAGSDDAPGSIAE"
     gene            complement(10955..12163)
                     /locus_tag="PLB65_05780"
     CDS             complement(10955..12163)
                     /locus_tag="PLB65_05780"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOQ78074.1"
                     /translation="MKILVLNCGSSSIKYQLIDMQTENLIAEGIVEKIGEDIALFTYK
                     STRFTKKKREMVIDNHEQGLQLILEALIDPVTGVLSGLDEIDAVGHRLVHAGEHYSDA
                     VVVTDDVIRVLQECVSLAPLHNPANLIGIEAVKVNMPNCPQCGVFDTAFHQTMPAMAY
                     LYPLPLEFYTNHKIRRYGFHGTSHKYVSLKAADYLKRPVEELKMITCHLGNGASITAI
                     KNGKSVDTSMGFTPLEGLMMGTRCGDIDPAIPIHLQQQLGFTVDEVNTILNKKSGMLG
                     LSHISNDMREIEDEILIKNNPKAIMAMDVYCYRIKKYIGSYFAALNGLDVLVFTGGIG
                     ENMSMLREQVCREMEAIGIKLNLEENSKRIDGIQVLSAEDSKVIVLKIPTNEELMIAL
                     ETESLLRQEK"
     gene            complement(12347..12919)
                     /locus_tag="PLB65_05785"
     CDS             complement(12347..12919)
                     /locus_tag="PLB65_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78075.1"
                     /translation="MKYVFLSVLIIGLATLSALSFEAENLNFFLNDNLWVMEGLYYFA
                     NYDTTAVSQLIYFPVLCDSVCSPAILEKIEIHEGTNCQITMLQQMDNGFSYLLFLPAK
                     ALCTVQIIYSQHLKGNYVKYILTTANKWGRPLPYAKYTLVKGKEVQITSLPFPVQEQE
                     ADKYVWEFYDFTPKKDWEVYFVPHREKSEE"
     gene            complement(12916..13923)
                     /locus_tag="PLB65_05790"
     CDS             complement(12916..13923)
                     /locus_tag="PLB65_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78076.1"
                     /translation="MKRLALFFLILCTFSVIWANPIVENLISRFWFEENNLLTIELSA
                     FIGLAQIETVTYLDAEDSLTITVNPNTTNVQVIYIPQSCNTPAQGYFTIRVFGMQDEI
                     VRWGEGLENDLSPLNGTQCGIQLLYSNLIGDSIYGWAKDTSPSACGPNYCSARFTYRV
                     ECTNLSGLPVAGVSVYHSQCCGNGFDNPAISDVAGIATDELWPMKTHLWVQNPLTSET
                     VFSTHFFAEPSELKVFSVQLPISVEDSVLPESEGRLSIHPSVISYSTGGVLHIAYDEK
                     ILGEAELILYDLKGQKICKTKYDKKEVDWQLPKLANGVYFVRLTSKGRALGTAKLIVI
                     K"
BASE COUNT         4530 a         2757 c         2554 g         4483 t
ORIGIN      
        1 atctgtggaa tctgtgtaat ctgtgagagg tatttaaagt tcaaagtgca ggagtgcaaa
       61 gttcaaagtg caggagttcg tagttcaaag ttattctttc ggtaacttcg gtgatctcgg
      121 tggctaatta taaaaaatcc ctaaaatccc tttaatcctg ttcatcccag acctattatc
      181 tatttttaat ctttgaaatc tgtaatctgt gagagacaat ccaaatttcc ccactttttg
      241 cattgacata attctgccat ctaaatttgt ggtataagta cgattaagat tttcaaggag
      301 aaaaagatag atgaagaaag caatacatcc taaatacgaa atggtaaccg ttacctgtgc
      361 ttgtggaaat acatttcaaa ccaaaagcac taaagataaa ttgcaggtag aaatctgcaa
      421 tgcatgtcat cctttttata caggtaagca gaagattatt gatactgctg gaagagtaga
      481 gaaattcaat aaaaaatata atcttaaaca ggaaaattag ggttttatac ccagtttacg
      541 ccattttatc cgttttctga aatcggggaa aatggcgttt tttctatctt aggaagaaat
      601 atgtctgaaa agaaagaaat tagcgtaggg ggccaagctg ttatcgaggg tgtaatgatg
      661 cgcggtccag atcttctggc aactgctatt cgccgaaaag atggcagtat tgaattgtta
      721 aaaacgccct ttgtcagcat tactcagaag aagaaatttt tggggctgcc gattattcgt
      781 ggttttattt ccttaattga aatgatgatt attggaatta agaccctcac tttttctgcc
      841 aatcgttttg agctggattt tatagcagaa gagaaagcca agggtaaaaa agtgaaaagt
      901 aaaaacaaaa ctgcagaaca agcggaaaac atcgtcagtt atataattgc ctttggctta
      961 gcttttctcc tgtttggatg gttaccatat aaacttgctg attgggttca tctttcgcgg
     1021 caggattttc tgtttaatgt ctttgctgga gtaatcagga taatattctt cgtactctat
     1081 gtttatttga tttccctgat gaaggatata aaaaggttgt ttcgctatca tggagctgag
     1141 cataaaaatg taaatgccta tgaacataac agtcctttaa taatagataa aattaaggaa
     1201 tggtctacta tccatcctcg ttgtggaacg agttttatct tttttgtgtt attaatcagc
     1261 atcctgatct tttccattgt ggatacttta gtttcagctt ttattttgca ttctccagtt
     1321 ccagtttatt tacgcttagc atatcatatt ttactgatgc cattaatttc gggtgtttct
     1381 tatgaagtgc tgaaattctc cggaaaaaat ctaaaacatc ctatcgtaaa acttttaacc
     1441 gttcctggga tggctttaca acatataact actcagccac ctgatgatga aatgctagaa
     1501 atcggtttag ttgctatgaa ggctgctctg gatatggata taagtgatca taatgtaaaa
     1561 atcgtggagg aataacactc ttgctacctc tcgaaaaatt acaaagcctg gaaaatgaac
     1621 tgaatgaaat gcaggaacaa atcagcgatc ctgctttgat caatgatact aaaaagtacc
     1681 gtgattgctt gcgccgcttt aaagaactaa aagaaattga ttctgcctgg aaacattatc
     1741 ggaaactgga aaaacatatg gaagaagtga aggaactgga aaaaagcgaa aacgatccag
     1801 aaatgctaaa tctaattaaa gatgaagaaa actctttgca aaaccaactt gaacaggcag
     1861 aactaaaatt gcgcgatttg cttattccct ctgatcctaa tgattccaaa aatgccattg
     1921 tggaaattcg ggcaggaaca ggtggagaag aagctgctct ttttgctgct gatttattta
     1981 gaatgtatag ccattatgca gaaatgaaag gctggaaact ggaagtgatc tcaatgaacg
     2041 aaaccgaact gggtggctat aaagaagttg tttttcagct tagcggtaaa gatgtatatt
     2101 cctatatgcg ttttgaaagc ggggtacata gagtgcaaag aattccggta acagaatcct
     2161 ctggtagaat tcatacttct gctgttactg tggctgtttt acctgaagca gaagatatag
     2221 atatagatat ccaggatagt gatttgcgca tagatgtcta tagaagtaca gggcatggtg
     2281 gacaaagtgt taatacaact gattccgcag tgagaattac tcatctgcct acaggaattg
     2341 tggttacctg ccaggacgaa aaatcgcaga ttaaaaacaa agctaaagcc ttaaaagtgt
     2401 tacgcagccg tttactggat gctgagataa gccgtcagga acaagaaatt gccagagcta
     2461 gaaaagctca ggtctcaacc ggtgaccgca gtgcaaaaat ccgaacttat aattttccac
     2521 aatcccgagt tacagatcat agaattaact tgacaagcta caatctagat tcctttttgg
     2581 caggaaatat agaagagttt attcaggctt tacgaattgc ctggagaaac gagaaggtga
     2641 atacataaat ggcagtggct cagattgttc tgatcctgat cgtattactt ttttttctgg
     2701 tgcttagttt tttcttttct ggcttggaaa cgggaatgct ttctatagac cagataaaat
     2761 tggaacaaga agccaaaaag agtaaaagca aagctgttct tttgcagttt gtccgtcaac
     2821 ccgataaatt tttaggtacc actttaattg gtaataacat tgctaatgtg attattgcct
     2881 ctctttctac ctatttagca aatcgactct ttgcccctgt ctttgatgct cgatacactt
     2941 ctttggtagt agggatagtt gtgctgatat ctggagaaat gattcccaaa actattttca
     3001 gagataatgc cgaaacgctg atccctttgt tttttcctct attgcaattt ttctattatc
     3061 tgctgaaacc gttggtaatg atagttacct ggctgaacaa tttcttgcgg aaattgctga
     3121 aactggaaga gggatatcag tacgattatt taacaaagga tgacctctcc tttctgcttt
     3181 cccaaaccgc tatagatgaa acaaacgatc accaaattga gatgattgaa gatgccttgg
     3241 atttttcaga actggatgcc agaaatgtta tggttcctcg tacagacatt gttgccattc
     3301 aagataaaac ctctctatct gaagtagcca atattgctcg taaagaaggt tttactcgct
     3361 atcctgtata ttgtaacaac attgatgata taatcggaat tcttattatt tatgatattc
     3421 tgaaacagga ttgctctcct gatatgactg caggagatat tattcacgaa cctctcttcg
     3481 ctcctgaaaa tacagatctg gatgtccttt tgaaagaaat gcaaactaaa caacgcagta
     3541 tggctattat tgttgattcc tttggaggaa cagccggaat tgttactatg gaagatatcc
     3601 tggaagagat cgttggtgat attgaagatg aatatgatac cgatgaagat gaaaactctg
     3661 ttcaacaagt tggaccaaag acatggctgg ttgccgctga tactgaaatt gatagattga
     3721 tagatgatta cggtattcaa ttgcccgaag gcgattatga aacagttgca ggtttaattt
     3781 tagaccgctt ggaaaaaatc ccgcatcagg gtcaggtagt taatgttcct ccttatacta
     3841 tacaggtttt acaggcaact tcaaaacaga ttgtaaaggt taaaatacat aaaataaatt
     3901 agatagaggt tcaattatga aactgatgga atgtgtcccc aatttcagtg aaggaagaga
     3961 cagctccatt ctaaatgcta ttgcagcagc tattaaaagt gttaagggag tggttttatt
     4021 ggatatggat ccgggcgctg atacaaacag aactgttttt accatagctg gagaacccga
     4081 agcagttgtg gaagcagctt ttcaggcaat taaaaaagcc gctgaactga ttgatatgag
     4141 taaacataaa ggagctcatc ccagaatggg agctaccgat gtatgtccct tcatcccaat
     4201 ttcggaaatg acgatggaag aatgcgtaga atatgcaaat aaattaggaa aacgagttgg
     4261 cgaagaactt ggcatacctg tttacttata tgaatatgca gccactaaag aagaatggaa
     4321 aaatctggct aatatccgca gcggggaata tgaagcacta tctgaaaaag ctaaagaccc
     4381 atattggaaa cccgatttcg gtccccatac ttttaatgcc aaaagtggag ctacagctat
     4441 tggagcgaga gaatttctaa ttgcctataa tataaacctc aatactcgcg acaagaaaaa
     4501 agcaagtgaa atagctcaaa taattcgcga aagcggaaga gcagcaaggg ataaaaatgg
     4561 taaactgatg aaagatgaaa acggaaatac catctatatc cctggcctat ttactcactg
     4621 taaagctgtt ggctggttca ttgaatccta cgatcgcgct caaattagta taaatcttac
     4681 caattataaa attacccctc cccatctggt tttggaaaaa gtgcgcgaac tggccagcga
     4741 aatgggcatt caagttactg gcagtgaact ggttggctta atccccaaag cggctatttt
     4801 agaagctgga aaattttatt taagtagaat gaacgaaagt accggtatcc ccgaaagaat
     4861 gattatgcaa acagcaattc aatctatggg cttggcagaa ttagctccct tcgatataga
     4921 taaaaaagtt attgaatatg ccatctccag aaaagatcga ttggtaaacc tgaaagtgga
     4981 tgccttctgt gatttgcttt ctactgatga acccgctccc ggaggtggaa gcgttgctgc
     5041 tctttgtgca tctatgagtg gagctctttc tgcgatggtt gcaaatctta ctattggcaa
     5101 aaaaggatat gaaaatgtgg ctaaagaagc tatggaacta gcccctattg gacaggaaat
     5161 aaaagaaaag tcgctggaag tgattgataa agatacagat gccttcttca ctatgatgga
     5221 tgctatgcgt ttacccaaaa aaaccgaaga agaaatagaa tttcgcaaaa ctcaaattga
     5281 aaaatgcact caagctgcaa ttaatgctcc tttggaaact atgcacttag caatgtccgc
     5341 tgccgaactt gccttaaaaa tagctaaaat tggtaatgcc aatgctcttt ccgatgctgg
     5401 agtggctgct ttaactgcaa attgcgcttc tcaagctgct tactataata tcctgattaa
     5461 tcttcccgga attgctgatg aaaattatat acataaaatc cacgccgaag caaaagatct
     5521 gctggaaaaa tgtaatttgc tttgtgatag cgtagaaaaa gatgttttgg ctaagttaat
     5581 gggtgaaaaa gtggaaaggt gaaaaagtcg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5701 nnnnnnnnnn gtcgagaagt cgagaagtcg agaggtgaaa cccacgattt aatttgtgga
     5761 aaataacctt ctttataagg agtcctcact gccaaacgag ttataggtat gttggaaact
     5821 tatttcggtg gtaaggactc tatctttata taaatactcc attacttttc ctaacctaat
     5881 aaatctatct tttcccttat tttttctctc tctgatacac agttcagcta ttttaacttt
     5941 ttctaattgc ttgttggaga ggtaagccca atttacaaga gttaaaagat gaacgaaaaa
     6001 gagggtttta ggacagcaaa aaaaggtaaa atccagataa aaacaccgaa tatgatcgaa
     6061 tacatctccc ctttagttaa aaaatcatcg agattgtgtc cttatcgcta attgttcaat
     6121 attctttgtt tgtagatttc ggatactttt tgcaggaagt cctctttatt atcataacgc
     6181 ataacacttc ttgccgaagg agaaggcaaa atatcaatct ttatttctct ttgaaccccg
     6241 ctttttagaa ttagcttagt ttggttttcg tccattttca ctaaatccaa tttttctttt
     6301 tgctggaggt agtccactaa ccaataatga actctgtttc tgctggtgca gagaatttta
     6361 ttgagggtgg gacggctata caaaattgtt aaaatatcct gatattttaa aactttcagt
     6421 ttataatcct ctgcgctggt atccttgcgt ataaatttca agaggatatc tgccattgcc
     6481 agcttatttt cggctaagaa gctttgtaaa aaattaacct gataagcagt tctttttatt
     6541 ctctcgtctt gcctaaatct taattccgaa atacagttta ttgattctgg ttttagagcc
     6601 aaaaacaaaa tatcccaaag caaattgctg ttgctgccat agtagtagtc aatatcctta
     6661 tttttaagat cattgatata tcctgtgcaa aaacgatagg gaggagcact tcccagaagt
     6721 aaaactttag cttcttcagg caagaaaggt ttatagggat gggtttgtat ggtttccata
     6781 tatcaaaaag tgattaatct atagtctcgt cttcgttaat aatttcatct tccggtaaag
     6841 gtaaatcggg gagagctgcc attctttttt tcctgattcg ccaggcgata aataaaataa
     6901 tcggtaaaag tatagccaga agagcattaa agctccaaaa cagatattga taacttaacc
     6961 gcttggtata ttgagaatat tccttatgaa aatcatatag tgtagttctg taagcacgga
     7021 caaagcaatc ggtgaaagat gccttagttt tttttctctg atagctcgct gccagttccc
     7081 aaaaatcctg ccattccttt ggtttttttt cactcatata gcgaacagcc attgccgaag
     7141 ataaatagaa taaagaccaa tcttcttttt tttctggata gctataactg agccgaaaca
     7201 gatcactttt cttccccaaa aaactctgtt ccaaataata aataaactgt tcaaaaccta
     7261 cctgctgagc ataatgcgta gccatccctt catgaaacca taaaggtgcc tgttcaaaaa
     7321 tttgctccag ataccaatga atatattcat gcatcaaaac cttgcgatga ttttcttgaa
     7381 ttgaagcaag aggacgcaca tagattcttt gttctgaccc actatagaaa gcatcactaa
     7441 attccactat ttttgccttc cctaaagaaa gagtttgata acttgtatta ttttcaatca
     7501 ggtagacagg cgcaagcaga tcaagatata ctcctatatt catctgaaaa ctatcaatat
     7561 tatcatctaa ggacttcaac attgtttcca aaggaacgga aattggactt tctgcgtata
     7621 taacaagatt attagttttt agctgcaggg tgtagtttgc ataaaggtat cccgcaatca
     7681 ataacgaaag caagataaaa aagcgcttta gcaatttctt attcctttca tttgttacag
     7741 caattccttt ctgcttctga aggaataaca taaaagcaga aaaaaggaca tagctagatg
     7801 cccctgatca ggaaaggtct tctttgcgta acaactcgta atatttcgtg ctgtcctttt
     7861 tcgctacatt tccttctgga atttgcgtta tccggatttc gtgttccgca ttataaagcc
     7921 gaaaaataat aatatcacca gctttaatta tcgtggatgc ctttgctggt tttccattaa
     7981 ctaacaccag gtttttatcg caggcatttt tagctatgga acgggtttta gttaggcaca
     8041 gtttattcag caatagatcc aggcgcatcg tcactccctg cagaatttaa aatatcctta
     8101 atccggatag cgatatcttt tattttgaat ggtttcataa tataatctcg aataccgcgt
     8161 tgtcgagctt cttccataga aattagggtg gtaaacccag tgcagattat tattggaata
     8221 tcgggtttta tagtcatcag tcttgcagca agatcaacac cattaatgta aggcatcgta
     8281 gtatcagtaa caacaatatc tacagcatca ggattcttac taaaaaattc ttctgcttta
     8341 cgcgggtcgg taaatccttc cactttataa cctaagcgca tcatcccttg tctaaaaaca
     8401 gaaactaagc tcggttcatc atctacaaac attatacatt cagttccgct gtagtcaatt
     8461 tcgtctacaa tttcttcagt gctatcctcc cgtctctgtt cagatgtata taaaggtaaa
     8521 tacacatcaa aagtagttcc aaatccgggt tcacttttca ctctgatagc tccatgatga
     8581 gcctgaataa tactatgtac aatggaaagc cccaaaccag ttccatctgt agcaccttta
     8641 gtagtaaaat agggatcaaa tatgtggtct atgatagaag gttctattcc cgtaccatta
     8701 tcactaaccg atattttcaa atattggctt tgttccagtt ccggaaatgc ctgcatatca
     8761 ctactgcgaa atgaaacatt ttctacggat atttcgatca aggcatcatc tctttttaaa
     8821 gcttgcatag agtttgtacc aagattaatc actatttgat gtatttggct cggaaccgca
     8881 agcacagaac ttttatcagt tttataatct tcttttattt ggatagaacg cggcatatag
     8941 gacttcagaa aacggactga ttctcttaca tgatcaacca aatcaattac ttctgtttta
     9001 gattcttcct gacgggagaa agaaatcagc tgtttaatca tttccttagc tctggtggca
     9061 gattttaaaa cctcaagtaa attcatcgcg gcatcgcttt caggaataag atcatctgca
     9121 gccaattctg tataaccaac aattgcactt aagatattat taaaatcgtg tgcaatacct
     9181 ccagttaatc gtcccagcgc ttccaaccgt tgagatcttt tcatttgttc ttcttgcaaa
     9241 atttcataag taacatcttt acagctggca attatactgg aaaggcgtcc ctcatcatca
     9301 ataacaggtt tcacaatcat atctaaagta gctttagagc catctttcct ggttaaatca
     9361 acttttcccc gccagggctg tgaagaacga acacgcatcc aagattgttt catattccat
     9421 cttttactcg attcaaaagg aagaaattcg aagggtttat cgatcaaatc aattttacga
     9481 aaaccgataa ttttctcaaa aatggaattt acatatttaa tatttccttc cggagaaaag
     9541 attatagtgc cttctccggc ttgttcaatt gcctgaatta ggattttaat actatcatct
     9601 gtctgcttct tatgcaaagc aaaggcaatc tgttcagaaa cagattccaa cagcaactga
     9661 tcttcttcat catagagatt tgtttttgtg tatgactgaa ctactaacgc cccgatcaca
     9721 ttcccggaga ggattaaagg aatccccaaa aagtttttgc attcaactcc ataagctcca
     9781 attattttaa ctctatcctg aatttcgtca gcatccaaaa gcaaaggtct ggcttcatta
     9841 attatctgag ccgttagaga tttactgtca tcagccacaa ccggagaagc attttcttcc
     9901 atttcatcaa ccagataagg gaaggtaatc atattgtttt tcccatcgta tagagcaata
     9961 tagaaatttg tggcatcaat aatcgttgct aaggaatcgt ggataatatg aaaaagttca
    10021 tccaatgact cagtagtatt aactgcatgg ctgattttat gaagaatagc agtaattctt
    10081 tcagaacgat aagccggagt gagatctgta gaaataaaag catacagagt tacatccttt
    10141 gtagaaattg gcatcaaagt gtgtttgcgg gcatatactt tatggcggct atccagaaag
    10201 gagaattgtg cttctcgggg tctatcctga tgttcggaaa taactcttag taccttttct
    10261 ttgctcaatt cataactggt ttcgtatgta actttcttgc caaattcctc ataaacatcc
    10321 cacagatatg aaccgattat tctatttatg ggtataccaa gatgttctcc cattgtttca
    10381 ttagccatca taatcgtacc agaattatca agcaacagca ttccttccga ggactgttca
    10441 aaaatacaac gaaacagcgc ttctcttttt tccaattcat tactggttaa agttaacttt
    10501 gtcatatcct gcatagcaac aatcacagtt tcatagtctt tctgttcact ttcaggaatg
    10561 ctccaagtaa ttttaaaata cagtttgttg ccttccagat cataatttat accctgccct
    10621 tcaaaatact cttttccttc atcaaccgca ataaaagact ccagtaaata ttcttttact
    10681 tcacctttaa atactagctg cagattattc aatagctcct gcttatcttt agctttataa
    10741 agagatagag ttgcgttgtt tacatccaca actactagtt tatttaccaa agcatccagt
    10801 tgatcaggat gcaatttcag atgagttcta agatcagaaa tacccctttt tttcattttt
    10861 tccagttctg cctggattcc agtaaaatct tcgatccata aagataccgg tgacttgtca
    10921 aacagcatcc ggtaaaatga ctgattcatt ttgttcattt ttcctgcctt aataaacttt
    10981 cagtttccag agcaatcatc agctcttcat tggtaggtat tttaagtaca ataaccttag
    11041 aatcctctgc actcaaaacc tggataccat caatgcgttt tgaattttct tccaaattta
    11101 attttattcc aattgcttcc atttcgcggc aaacctgttc tcgtaacata gacatatttt
    11161 cacctattcc gccagtaaaa accaggacat ctaatccgtt taaagctgca aaatagctgc
    11221 ctatatattt ttttatccga tagcaatata catccatagc cataatagct tttgggttat
    11281 tcttaattaa tatttcatct tcaatttcgc gcatatcatt ggaaatatgg cttaagccca
    11341 gcattccgct ttttttgttt aaaatagtat taacttcatc aactgtaaac ccaagctgct
    11401 gttgcaaatg aatcggaata gcaggatcga tatctccaca acgggttccc atcattagtc
    11461 cttccaaagg agtgaaaccc atagaagtat caactgattt tccatttttt atggcagtta
    11521 tggatgctcc gttacctaaa tggcaagtga tcattttcag ctcttccaca ggtctcttta
    11581 aataatcagc cgccttcaga cttacatatt tgtggctggt tccatgaaat ccataacgcc
    11641 gtattttatg gtttgtgtaa aattctaaag gtagaggata taaatatgcc atagcaggca
    11701 tcgtttgatg gaatgcagta tcaaaaacac cacattgagg acaatttggc atattaactt
    11761 tcacagcttc tatgccaatt aaattagcag gattatgtaa tggagccaaa gatacgcatt
    11821 cttgcaatac cctaattaca tcatctgtaa caacaaccgc atcggaataa tgttctcctg
    11881 catgtaccaa tcgatgacct actgcatcaa tttcatctaa accactcaag acaccagtaa
    11941 ccggatcaat taatgcttcc agtataagct gcagaccttg ttcatgatta tcaattacca
    12001 tttcccgctt ttttttggta aatctggtgc ttttataagt aaaaagtgca atgtcttccc
    12061 cgattttttc tactatgccc tctgcaatta agttctccgt ttgcatatca attagctgat
    12121 atttaatgga tgaactacca caatttaata ctaatatttt catctttcct caacttattg
    12181 aaattagttg tgtgtctttt cttcttttct tgaataactg tcaaatcctt ttttctttct
    12241 ttttgcattt tatcgtaatt atccttttat ctccttgatt atctatctgt tgctaaaatc
    12301 ttccaactct gataataaaa aaacagcaga gctaattagg cattttttat tcctcgcttt
    12361 tttccctatg aggcacaaaa tacacttccc aatctttctt gggagtaaaa tcatagaact
    12421 cccacacata tttatctgct tcctgttcct gaaccggaaa aggaagagaa gttatttgca
    12481 cctctttccc ctttactaaa gtatattttg catagggtaa tggccttccc catttatttg
    12541 ccgtagtgag aatgtatttt acataattgc ctttcaggtg ctgagaataa ataatttgca
    12601 ccgtgcataa agcttttgca ggtaggaaaa gcagatatga aaacccgttg tccatttgct
    12661 gcaacatagt tatctggcag tttgtacctt cgtgaatctc tattttttct agtatagcag
    12721 gtgaacatac actatcacat aaaacgggaa aatagattag ttgggaaaca gctgtcgtat
    12781 cataattagc aaaataatat aacccctcca tcacccataa gttatcattt aggaaaaaat
    12841 taaggttttc cgcctcaaaa ctgagagcag aaagggttgc taacccgata attagaaccg
    12901 acagaaaaac atatttcact tgataacaat cagtttagct gttcccaatg ctctgccttt
    12961 gctcgttaaa cgcacaaaat atacgccatt agcaagcttg ggaagctgcc aatccacttc
    13021 ctttttatcg tattttgtct tacaaatttt ttgccccttg aggtcatata aaatcagctc
    13081 tgcttctcct aaaatcttct cgtcataagc aatatgtaaa actcctcccg tactataaga
    13141 tattacggaa ggatgaatag aaagcctccc ctcagattca ggcaaaacac tatcttccac
    13201 agaaataggt aattgcaccg aaaaaacctt taattccgat ggctcagcaa aaaaatgagt
    13261 gctaaaaacc gtttcactag ttagcggatt ttgcacccaa agatgcgttt tcataggcca
    13321 taactcgtct gtagcaatac ctgccacatc agatatagct ggattatcaa atccatttcc
    13381 acaacactga ctgtgataaa cagaaacacc agcaacaggc aatccactta aattagtaca
    13441 ttcaacccga taagtaaatc tggcagaaca ataattagga ccgcaggctg aaggtgatgt
    13501 gtcttttgcc caaccataga tactatctcc aattaaatta gaataaagca attgaatacc
    13561 acattgagtt ccgtttagcg gacttaaatc gttttccaaa ccctctcccc aacgaacaat
    13621 ttcatcctgc attccaaaca ctcgtatagt aaaatatcct tgcgctggtg tattacagct
    13681 ttggggaata taaattactt gaacattcgt agtattagga ttaacagtta tagtaagaga
    13741 gtcctcagca tcaagataag ttaccgtttc aatttgagct aaacctataa aagcgctcaa
    13801 ttcaatcgtc agtaaattat tttcctcaaa ccagaatctg ctgattaaat tttccactat
    13861 gggatttgcc caaataacgg aaaaggtgca aaggattaaa aaaaatagag cgagccgttt
    13921 catttttctt ctcccccttt tttataatac cataattata agcaactttt attccagaac
    13981 ctgcttttat aatcattgct ggaaagaaga taccttttta gcgcacaatt aaaatagcca
    14041 ccgtcccctt tattatacaa ataggcaaat taccgtccct tttatgcaac ataataatct
    14101 ctcggtaatc acgcagattt aatagcctgg aataaaaaag actatctgaa tatctgaaaa
    14161 ttggatatta ggaacgcgat tacaggattt caaggataaa gtacttattc cgtagaaacg
    14221 atagatatcc gcaacaggtt tccacccagc aataaacact cctataagaa aaactatttc
    14281 cgccccttaa aacctggatt gtagataaaa atagtcctgt gccaattccg ggcttgacaa
    14341 aaaatccctg ctgggaattt tgacaattaa agtgggtgat tagctcagtt ggttagagcg
    14401 ctacgttgac atcgtagagg tcac
//
LOCUS       DAOSTH010000041        13978 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_4447_length_13978_cov_17.141133, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000041 DAOSTH010000000
VERSION     DAOSTH010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13978
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4447_length_13978_cov_17.141133"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="PLB65_05795"
     CDS             complement(<1..422)
                     /locus_tag="PLB65_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78077.1"
                     /translation="MKVKYRNALKSYQGKNDGMVYYYNSRLKKSICRRYAYPKITQNN
                     LHFAQINHNLKELNPSEGYKSDLKMYANIIKADKKEKGYICWNNVYRKLMFALAKDKD
                     LDLASLTREDIYSQNLPCKTVKESIEAGYLSPVEGYQT"
     gene            1065..2396
                     /gene="dnaA"
                     /locus_tag="PLB65_05800"
     CDS             1065..2396
                     /gene="dnaA"
                     /locus_tag="PLB65_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOQ78078.1"
                     /translation="MDQDIWQNILDKLEENINPQSFKTWFSDTRLVDMNDKELMIRVS
                     TQFSANYLNQNYKEILSEISYSLYGRKYDIQFITQPRHNHNYNSDYPEIKSNRVTSNV
                     QLNERYTFDEFVVGKNNNFAYSAAKAVAESPGYTYNPLFIYGESGMGKTHLMQAIGNF
                     VLKEGRNSSIYYITSEAFTNAMIDGIRSNKMPEFREKFRKVDLLLMDDIHFLSRKEGT
                     QEEFFHTFNALFDNKKQIVLTSDRPPKDIPDLEKRLVTRFESGLLCDLKNPDFETRVA
                     ILRKKAEPESVILADEIINFIADNITSSVRALEGSLIRILAFSSYNKINPEQIDVSMA
                     EDILSDMISEKTYSITLDAITQEVCKYYQITPAQILEKTRKPQVAFPRQVAMYLANYL
                     IPQLSLKEIAEYYNRKDHTTVIHAKKMIENQFREKEDFRIQLEMMIKNLQK"
     gene            2854..4080
                     /locus_tag="PLB65_05805"
     CDS             2854..4080
                     /locus_tag="PLB65_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78079.1"
                     /translation="MESFRTFSKAFCCLMLVLIMISGCTDKKNLTGTNFSNVNAKSVN
                     DKEGIIMGYSYPAESQTKISGSETKLLAGNYQNAVALSFLRFTELPSTIESVVSDSCF
                     LTINIIKRSMLPRNPLKLQLFKLNKIWNDSLDLSNLSAGDLSASPLAEYTITDTIGIS
                     SKEITFPLSVSDVFSWSEPDSLGWNFVIKAENEGWVEIASLEGTNKPKLNIKYRKDSE
                     SAYLNYSKTPAKDTYTLNAPETTACADWKISNLNSSRLYLKYDPTYTIFRDNEDNILD
                     DTQIKRMTVNKAEVVLHIKNNDYYNSYTSYSLYSFNVIRDSINSPVPLVTGDYEIIAY
                     TVTSTGIIKGDSLTVDVTPIIQAYTSGDKTPKGIMIQSLQERKNFGSLEFWDCNEGTL
                     PEKKPYIKITYTPPFL"
     gene            4083..4883
                     /locus_tag="PLB65_05810"
     CDS             4083..4883
                     /locus_tag="PLB65_05810"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ78080.1"
                     /translation="MKKYIIIFVASILLFSCAKKEEKGIALAQVDKEVLYLEDFKSTF
                     SLEDWDNLSPEQRKKYIEDWVNLTLLSQYADSKGLDKDKAVKQKISYATKKVKANALI
                     AKRLSQIEVSEDQLFNYFRLHQGEFKKNAVEYNIQRIALVDKISAENVLQQLNQGMNF
                     AEAVQRYSTEELKYRGGYMGMVSSNGPDSLFWLKAREIKENEYGLVYKEPTWYVFRVL
                     ETKETLQEANFDDYRAEIKRKYLLEKQDQVYQDLIKEIKSQTSEVYYY"
     gene            5000..6256
                     /locus_tag="PLB65_05815"
     CDS             5000..6256
                     /locus_tag="PLB65_05815"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ78081.1"
                     /translation="MRKLTITLILIIFSLMLSAELVDKIVAKVGTDIILLSDLQKELA
                     QMQRAGYLDENTDPRMVLEEMVNQKLIIQKAKDLKLTVNEDEIKSMAENYLKKIKAQY
                     PSPAAFSTDLKNSKLTESDLLQFYKDMLTEQSLIDQILKREITSKVSVSEAEVEHFYN
                     ETKDSLAVKPVSWELGLIVREIKPSEATKQAKLAEIKEIQNRLKNGEDFATIAKNESD
                     CPSKEVGGDLGFVKKGQMVKPFEDAAFALKLGEISDIVESNYGYHIIKLEEKKGEEIR
                     VRHILKMLSSTAEDSLRERQLMEEIRTRYANGESFASLAKEYSMDKESSEDGGSLGEF
                     TEKDIPPLFSVQIMQTPVGEMTPVLENSGLLYIFCRLQEYPPRMYSFNEVKDQVRDLV
                     LRMKQNEAYNSWIANLRKEAYVQITL"
     gene            6256..6750
                     /locus_tag="PLB65_05820"
     CDS             6256..6750
                     /locus_tag="PLB65_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOQ78082.1"
                     /translation="MTEKQKKLNIYTFLAAFLGIGFIPFMPGTFGSLAAYGIYLLPKG
                     ELFIGTGLLYFIPAWLIFCLLAVYLSGKAEKTLGKDNGAIVIDEVCGYFVSVLFLPHN
                     WLIGLYAFALFRVFDIAKPFPIMRAQKIPAGWGIVIDDIIAGIYANLLIQILIKIYPR
                     FFGL"
     gene            6759..7112
                     /gene="yajC"
                     /locus_tag="PLB65_05825"
     CDS             6759..7112
                     /gene="yajC"
                     /locus_tag="PLB65_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HOQ78083.1"
                     /translation="MNYILLQATGKAATQPQQSGTGTTLIFMIIMFVILYLLLIRPQQ
                     KKAKETQKMQDSLKVNDKVLTSAGIYGRIVSLKPDKGIVVLEIDDNNKVRIDIQKNAI
                     VGVLNPSEPTPKNEE"
     gene            7102..7665
                     /gene="def"
                     /locus_tag="PLB65_05830"
     CDS             7102..7665
                     /gene="def"
                     /locus_tag="PLB65_05830"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOQ78084.1"
                     /translation="MKNRPNLLPVRLYGDDLLRKKLPELDFNTEGLQEFIDDLIYTMY
                     KRDGVGLAANQVGSEWRLFVIDPDWANSENDNKNPIVMINPVIESRSGEIVYEEGCIS
                     LPDIFGSVKRSANISYSYTDRYGKRITENAEQTKAVVIQHEYDHLEGILFVDHLGTLA
                     RLKIMHQLKRLQARAKDGQNILEGLSL"
     gene            7665..8609
                     /gene="fmt"
                     /locus_tag="PLB65_05835"
     CDS             7665..8609
                     /gene="fmt"
                     /locus_tag="PLB65_05835"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOQ78085.1"
                     /translation="MRYLFIGSSEYGLPALNKLIQNGYKPSLIISQPAKPAGRNRHII
                     PTPISEFADKQNLPLWTPENINSEEAIAKIAKYEAEIIITAAYGGYFKKNIRCLYPLG
                     TVNLHPSLLPKYRGASPIQSAILNGEKETGTTLYKVTAKMDAGPILLQKKLPILDNET
                     YSELNERLKQQAADLLWDFWKMLLKKQELMPTFQDESQASYCSKIDNNTCRINWQKTA
                     KEIHNQIRAFSLTPGAWTYFRSKKLKILASEKTKENTEGEPGLICGWDKNRGFFVNCL
                     DYKLLITKVQAAGKKVLSASAFANGARLAAKEKLGSDQ"
     gene            8606..9355
                     /locus_tag="PLB65_05840"
     CDS             8606..9355
                     /locus_tag="PLB65_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HOQ78086.1"
                     /translation="MKKEYFAIIKFPMFVGLSLLAILLIFFLMVLGSYHHLGLSGWGN
                     FFYTLIFILIIILIASWILTLISTHCKIKSTWLRKYISWMLVRVYYPLAIWISIPFFQ
                     KRASIQESFLYFNNEIIVTNYQGIHFSNLLLLLPHCLQNSNCKIRISSDIVDCQECGG
                     CDIAKMKSITRDNHIKAAVASGGSLARKLVNDTKPDMIIAVACHRDLTEGVRDSWRYP
                     VYSVLNERPKGPCFETTVSYSVIDFAIKKFI"
     gene            9368..10204
                     /locus_tag="PLB65_05845"
     CDS             9368..10204
                     /locus_tag="PLB65_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOQ78087.1"
                     /translation="MKRINLFTVILILLLNAFITMALINKYMDKSVAIDNSYTEYSSK
                     PSKASAANRINSITEAVKAVEPAVVSINVIKTEYVRARSPFGFGFFDFFGFAPMMRQV
                     ESIGSGVIYDPKGYIITNAHVVSGATQIKVILPDKREYEGVIAGIDEIHDVAKLKING
                     NNLPVARLGNSNDLIIGEWSIAVGNPYGFLMNDSKPSVSVGVISAVSRNFAARTDKRE
                     YKSMIQTDAAVNPGNSGGPLVNINGEVVGINTFIFSESGGNIGIGFALPINSVKTILA
                     KI"
     gene            10225..12924
                     /locus_tag="PLB65_05850"
     CDS             10225..12924
                     /locus_tag="PLB65_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOQ78088.1"
                     /translation="MLIFIAGSLLCAQITHSCYDIQYNPEGGSNSPYLDQVVTVQGIV
                     SGISFYSGTSVNSYGFFIADAAGGPWSGLYIYNQTYHPNLGDLVQITGTVNEYYGLTE
                     ISQLTSFQVLSQDNPLPEPAEINSGALNNLSSGEQWESVLVKVLNVSVVTEPNNYQEF
                     YVDDGSGVCQIDDQFFGSGHSWSNITLGQVYSEITGIVDYAYNYYAINPRFLTDLVLS
                     GTTATLLIPHQAVSLHSSVTVPVNALGIDLASSYQSYSFNLAFNPNIISYQSVSTEGT
                     ISAPGTINASCTNGLLSVTYQSNSVISGQGVLLNLNFYATNTGTTVLALENVFFGNDS
                     IQSLIDGSLTVNSCYNSPGDTLTVIQRPLLNIPEIVIPGETLTITCLAPQNTTNWNAW
                     LKHSNKRINMPISNSQWLNNPNRWELVTTIPSVPIFELYDLEVSASGGISDITENAIQ
                     VIPTRKNNYYFVHITDAHLPNRLYYPNAGFETDSTAVEDFRAVMEDINLIHPEFVLFT
                     GDLVNEGELESFANQYWYGWTQKLLSEFDIPVYVTSGNHDIGGWNQTPPPAGSARRNW
                     WKYFGWNWLDSEDPNWLYHTQDYYFTYGNTIFIGLESYDNYDYWRLNIYGTTSFTDQQ
                     IAWLNSTLSLFPNYTKVLFHHYDFQDELNLSALGIDLALWGHIHSNNGSISNFPYNLA
                     TRSICDGNRAYRMVRVNNNQIIPYNTIYAGATGNNLSIQYFPSNYGVADSIRATIINN
                     QPLTFENAQIKFIMPSGMNGYRVQGGILEQVDRSGVNNVCYVRVSLSANSTTNISIAD
                     SGVENIDPLQVPKPLQIKSIYPNPLSLQGNIQLYSEKSLPTLTLQLYNLKGQLAYQQT
                     LQNINKGENTLAFTLPDSLGNGIYFLRLPEDKNTTQKILILKK"
     gene            12932..13939
                     /locus_tag="PLB65_05855"
     CDS             12932..13939
                     /locus_tag="PLB65_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOQ78089.1"
                     /translation="MPEKRPQTAKLLYIPWILKHLSYYVKLNTHILKKGVPLKEILIL
                     KFPFWFSDAKIPPLLSVDITDACNLKCNYCNNPFFPHPRTMMSKEVFEALIEHLKRQN
                     VSRIRISGGEPTLHPQFDTMLKEMSHYCKYLSIITNGQWKNKEITEKLLDCGLSFIEL
                     SMDAGGKEIYEKNRPGADYDLFLQNLKDLYELRNRKKTDLIIRIRLMLRPSTLSLEKE
                     DSHYWKQFSDCVLPQHILQHPETPPQEDVFMQDSILQNQLPVCTVTYRDLQIRPDGKV
                     PLCPAKGCAINLEDRIFIGDIQKDDILDLWNAPMLKQMRNAHRKRQGEILNLCRNCHY
                     G"
BASE COUNT         4695 a         2437 c         2653 g         4193 t
ORIGIN      
        1 aaggtttggt atccctcaac tggtgataga taaccggctt caatactctc ttttactgtt
       61 ttacagggca ggttttggct ataaatatcc tcacgggtaa gggatgccag atccagatct
      121 ttatctttag ccagagcgaa cattagtttt cggtaaacat tattccagca gatgtatcct
      181 ttttcctttt tgtctgcttt aataatattc gcatacattt tcaagtcgga tttatatcct
      241 tccgagggat taagttcttt taggttatgg ttaatttggg caaaatgcaa attattttgg
      301 gtaattttgg ggtaagcata tcggcggcat attgattttt taagacggga attgtaataa
      361 tacaccattc catcgttctt accctgataa cttttcagtg cgttacggta tttaactttc
      421 attttttctc tccttttaat tttgtcctct atatatatag ggggtgacaa atctggccaa
      481 gtggtgacaa aattgtaact ttttattttc cttagggtag ctcctcatat tcctgacttc
      541 ttctcctcca aaacgagggg aagaggagga gtcatagacg ggtagagggg gagaggagaa
      601 ggggagttta gctggcaaaa gcttatggga aaagaaataa aatttttatg atatcggtaa
      661 gtctccctac ctatagaatt tacagcttta tatcgcaaat ctccaaaata gcgatatata
      721 gctataaagt taaaaatagc agggaattat taattttact atttgctaac ttctatattt
      781 gttgaagttt agctacttga caaatcagag aatagatttt ttttgggcat agctgtcttt
      841 tgtctcagga aaagaattgg ataagttccc atagaattga gtgttgctct acatttgtgc
      901 ttaagctgtg gataacttgt ggataactca gggaaaggca gcgtcagccg ctattagttt
      961 acataattaa gtggttgaca attaacaaga tatctataat aaacttgttt cctgttttgc
     1021 gggaagcagt ttgtggataa ataaatttta caggatattg aattatggat caggatattt
     1081 ggcaaaacat acttgataaa cttgaagaga atattaatcc tcaaagtttc aagacatggt
     1141 tttcggatac aagattagta gatatgaacg acaaggagct gatgataagg gtttctaccc
     1201 agttttcggc gaattatttg aatcagaatt acaaggagat tttaagcgaa atatcgtata
     1261 gcttatatgg taggaaatac gatattcagt ttatcactca acctcgtcat aatcacaatt
     1321 ataattctga ttatccagag attaaaagca accgggttac ttccaatgtg cagttgaatg
     1381 agcgttatac ttttgatgaa tttgtggtag gtaagaataa taattttgct tacagtgcgg
     1441 ctaaggcagt tgcagaatca cctggttata cttacaatcc tctttttatc tatggtgaaa
     1501 gcggaatggg taaaacgcat ttaatgcaag ccattggcaa ttttgttttg aaagaagggc
     1561 gtaatagttc aatttattat attacttccg aggcgtttac caatgcaatg attgatggaa
     1621 ttagaagcaa taagatgccg gaattcaggg agaaatttag aaaagttgat ctgttattga
     1681 tggatgatat tcatttttta tcgcgcaagg aaggcacgca ggaggaattt ttccacactt
     1741 ttaacgctct ttttgataat aagaagcaga ttgtgctcac ttcggatagg cctcctaaag
     1801 acatcccgga tctggaaaag cggttggtaa caagatttga aagcggttta ctctgcgacc
     1861 tgaaaaatcc tgattttgag acgcgcgtag ctattctacg caaaaaagcc gaaccggaaa
     1921 gtgtgattct tgctgatgag atcatcaatt ttattgctga taatataacc agtagtgtgc
     1981 gtgctttgga aggatcttta attcgcattc tggctttctc ttcttataac aagataaatc
     2041 ctgagcagat agatgtttct atggcggaag acattctctc tgatatgatt tcggaaaaga
     2101 catattcgat tactttagat gctatcacgc aagaggtttg taaatattat caaataaccc
     2161 ctgcccaaat attggaaaaa acaagaaagc ctcaagttgc ttttccgcgt caggtggcta
     2221 tgtatctggc aaattattta attccccagc tatcattaaa agaaatagcg gaatattata
     2281 accgtaaaga tcatacaact gtgattcacg ctaagaagat gattgaaaac cagtttcgcg
     2341 agaaagaaga ttttcgaata caactggaga tgatgattaa aaatttacag aagtaagcgg
     2401 tggatatttg gaatatccag aggaaaattt aaagccggtt gattgataat ttacagctta
     2461 tattgaaaat aatagaccaa aaaccatata agctgagtta atatttttct tgattttaag
     2521 gattaaacaa agcttccttg aaatcgttat aaagatgttt atatatgtga aaacaggcta
     2581 aaaaagaagt gactttaaat agttatccac atttgtggat aggaaactgt ggatagttgt
     2641 gaataacttg tgaataaaag agttttgagt ttatgagtgt ggataagctg tgtagaaaaa
     2701 gccagtttgt ccacaagtta tccacattaa gaatagcgat cgatatttat ataagacagc
     2761 gatcaaagaa aaagcttgac caaatatccc catattcaca acgcctactt atattacttt
     2821 atattagatt tatattaaaa taggattata tttatggaat cattcagaac attttcaaag
     2881 gcattttgct gccttatgct cgtcttaatt atgataagcg gatgcacaga taaaaaaaac
     2941 ctaacaggaa caaattttag taatgttaat gcgaaatccg ttaatgataa agaaggaatt
     3001 attatgggat atagttatcc tgcagaatcc caaactaaaa tatccggcag tgaaacaaaa
     3061 ctgttggcag gaaactatca aaatgctgtt gctcttagtt ttttacgatt tacagagctt
     3121 ccttctacga tagagagtgt ggtttcagat agttgttttt taacaattaa tattataaaa
     3181 cgctctatgc taccgcgtaa tcctttaaaa cttcagcttt ttaaattgaa taaaatttgg
     3241 aatgattctt tggatcttag caatctttcg gcaggagatt tatcggcgag tcctttagca
     3301 gaatatacaa taactgatac aattggcatt tccagcaagg aaataacttt tccgctttct
     3361 gtttccgatg ttttttcttg gagcgaaccg gatagtttag gttggaattt tgtaattaaa
     3421 gcagaaaacg agggttgggt agagatagct tctttggaag gaaccaataa gcccaagcta
     3481 aacataaaat atagaaaaga ttcggaaagt gcttacttaa attatagtaa aactcctgca
     3541 aaagatacct atactttaaa tgcaccagaa acaacagcct gtgctgattg gaaaataagc
     3601 aacctaaatt ctagtcgtct ttatttaaaa tatgatccta cttatactat ttttagggat
     3661 aacgaagata atatattaga tgacacccaa attaagagga tgacggttaa caaagcagaa
     3721 gtagtgctcc acataaaaaa taatgactat tataattctt acacttcata ttcgctttat
     3781 tctttcaatg taattagaga ttctataaat tctcctgtgc cattagttac aggtgattat
     3841 gaaataattg cttatacagt taccagtact ggaataatta aaggagattc gctaacggtt
     3901 gatgtaactc caataataca agcatacact tctggagata aaactcctaa agggataatg
     3961 attcaatctt tgcaggagcg gaagaatttt ggaagtttgg aattttggga ttgcaacgaa
     4021 ggcacattgc cagagaaaaa accttatata aaaataacct atacaccacc cttcttatga
     4081 atatgaaaaa atacataatt atatttgtcg cctctatatt gctgttttct tgtgcaaaaa
     4141 aagaagaaaa aggtatagcg cttgctcaag tagataaaga agtgctatat ctggaagatt
     4201 ttaaatctac tttttctttg gaagattggg ataatctaag ccctgaacag aggaaaaagt
     4261 atattgagga ctgggtaaat cttacgcttt tatctcaata cgcagatagt aaagggttgg
     4321 ataaagataa agctgttaaa caaaaaatca gctatgccac aaaaaaagtt aaggcaaatg
     4381 cgttgattgc caaacgcctt tctcagatag aggtttcgga agatcaattg tttaattact
     4441 ttcgtttaca tcaaggcgaa tttaaaaaaa atgcagtaga atataacatt cagcgtatag
     4501 ctttagtaga caaaatatca gcggaaaatg tattacagca gttaaaccaa ggaatgaatt
     4561 ttgcggaagc ggtgcaacga tattctacag aagaacttaa atacagaggt ggttatatgg
     4621 gtatggtttc ttctaatggt ccggattccc ttttctggtt aaaagccaga gaaataaaag
     4681 aaaatgaata tggcttagta tataaagaac ctacttggta tgtatttcgc gttttagaaa
     4741 ctaaagaaac tcttcaggaa gcaaattttg atgattaccg ggcagaaatt aaacgcaagt
     4801 atctattgga aaaacaagac caggtttatc aggatttaat taaagaaatt aaatcccaaa
     4861 ccagtgaagt atattattac tgaaatgttt aaattaacca gatcaataag attgaattat
     4921 tatttagcaa acaatagagt ggcagatata gaatctgcct caaaacaaaa cctttacaaa
     4981 taaaaaagga aatattagaa tgcgaaaact tactataact cttatcctga ttatcttttc
     5041 cttaatgtta tctgcggaat tagtagataa gatagtagcc aaagtaggaa cggatattat
     5101 tctgctgtca gacctccaaa aagaactggc acaaatgcaa agagcaggat atttagatga
     5161 aaatacagac cccagaatgg ttctggaaga aatggttaat cagaaattaa tcatacaaaa
     5221 agctaaggac ttaaagctaa ccgtaaatga agatgaaata aaatcaatgg ctgaaaacta
     5281 cctaaaaaag ataaaagccc aatatcctag tccagcagct ttttctacag accttaaaaa
     5341 tagcaaactt acggaatctg acctgttaca attctacaaa gatatgctaa ccgaacaatc
     5401 tctcatcgac cagatattga aaagagaaat tacaagtaag gtatctgtta gcgaagcaga
     5461 agtggaacat ttttataatg aaacaaagga ttctttagca gttaaacctg tttcctggga
     5521 actggggctt atcgtaagag agataaaacc aagcgaagca acaaaacaag caaaactggc
     5581 agaaattaaa gaaatccaaa acagattaaa aaatggggag gattttgcca caatcgctaa
     5641 aaacgaaagt gattgcccca gcaaagaagt tggtggcgat ttaggttttg tgaaaaaagg
     5701 acagatggtt aaaccttttg aggatgcagc gtttgcttta aaacttggag agataagtga
     5761 catcgtagaa agtaactatg gatatcatat catcaaattg gaagaaaaaa aaggcgagga
     5821 aatccgcgtt cgacatatct taaaaatgct atcttccact gcagaagatt cattaagaga
     5881 aagacagcta atggaagaaa tcagaacccg ctatgctaat ggtgagagtt ttgcttcctt
     5941 agcaaaggaa tattctatgg ataaagaaag cagcgaagat ggaggttctt tgggtgaatt
     6001 tacagagaag gacatacctc ctcttttctc cgtgcaaatt atgcaaacac ctgttggaga
     6061 aatgactccc gttttagaaa attcaggttt attgtatatt ttctgtcgat tacaagaata
     6121 ccctcccaga atgtatagct ttaatgaagt gaaagaccag gtgcgtgatt tggtattaag
     6181 aatgaaacaa aatgaagcgt ataattcctg gatagccaat ttaagaaaag aagcttatgt
     6241 gcagattaca ctataatgac tgaaaaacaa aagaaactga atatctatac ctttttagcc
     6301 gccttcctcg gaattggttt tattcccttt atgccaggaa cttttggttc gctggctgct
     6361 tatggaattt atcttttgcc gaagggagaa ctttttatag gaacagggtt attatatttc
     6421 atcccagcct ggctgatatt ttgtctactt gccgtttatc taagtggcaa agcagaaaaa
     6481 accttaggta aagataatgg ggcaattgtt atagatgagg tctgcggcta ttttgttagt
     6541 gttctatttt tacctcacaa ttggctgata ggtctttatg cctttgcgct gtttagagtt
     6601 tttgatattg ccaaaccctt tccaattatg cgagcacaaa aaatacccgc tggctgggga
     6661 attgttattg acgatattat agccggaatt tatgctaacc tcttaattca aatactaatt
     6721 aaaatatatc caagattctt tggattatag gagataaaat gaactacata ctattacaag
     6781 ctacaggaaa agctgccacg caaccgcaac aatcaggaac aggaactacc ctgatcttta
     6841 tgatcattat gttcgtgatt ctgtatttat tgcttatccg tccccaacaa aagaaagcta
     6901 aagaaactca gaaaatgcaa gattccctga aggttaacga taaagtttta accagcgcag
     6961 gaatctatgg acgcattgta tcgttaaaac ccgataaagg aattgtggtt ttagaaatag
     7021 acgataataa caaagtgcgt atcgatattc aaaaaaatgc aattgttgga gttttgaatc
     7081 cttccgaacc taccccaaaa aatgaagaat agaccaaatc ttttacccgt tcgcctctac
     7141 ggagacgatt tattaagaaa gaaacttccc gagctggatt ttaatacaga aggcttgcag
     7201 gaattcattg atgacctaat ttatactatg tacaaaagag atggcgttgg tttagctgct
     7261 aatcaagttg gttcggaatg gaggttattt gttatagatc cggattgggc taatagcgaa
     7321 aacgataaca aaaatccgat agtgatgatt aatccagtaa tcgaaagtcg ctctggagaa
     7381 attgtttatg aagagggctg tatcagtttg ccagatattt ttggaagcgt aaaacgcagt
     7441 gcaaatatat cttattcata tacggataga tatggtaagc ggattacaga aaacgctgaa
     7501 caaacaaagg cggttgttat tcagcacgaa tatgatcatc tggaaggtat cctgtttgta
     7561 gatcacctgg gaacccttgc ccgcttgaaa attatgcacc aattaaaaag attgcaggca
     7621 agagcaaagg acggacagaa tatattggaa ggactatccc tataatgcgt tacctattta
     7681 ttggctcttc agaatatggg ttacctgccc taaataaact aatccaaaac ggctataaac
     7741 cctctctgat aataagccaa ccagctaaac ctgctggacg caataggcat ataataccaa
     7801 cacctatttc tgaatttgca gataaacaaa atttgccact atggacacct gagaacataa
     7861 attcagagga agccattgcg aaaatagcaa aatatgaagc agagattatt attacggcag
     7921 cttatggcgg atattttaaa aaaaatatcc gctgcctcta tcctttggga acggttaatc
     7981 tccatccttc tcttttacca aaatatagag gagccagccc tattcaaagt gcgatcttaa
     8041 atggtgaaaa ggaaaccgga actacgctat ataaagtaac agcaaaaatg gatgcaggtc
     8101 ctatcctact tcaaaaaaaa ctaccaattt tagataacga aacttacagc gaactgaatg
     8161 aacgcttaaa acaacaagca gcagatttac tttgggattt ctggaaaatg ctgctaaaaa
     8221 aacaagaatt aatgccaacc tttcaagatg aaagccaagc ctcctactgc agtaaaattg
     8281 acaataatac ctgtaggata aactggcaaa aaacggcaaa agaaatccat aaccaaatcc
     8341 gtgctttttc tttaactccc ggcgcctgga cctatttcag aagcaaaaaa ctgaaaatat
     8401 tggcttcaga aaaaactaag gaaaacactg aaggagaacc gggattaatt tgcggatggg
     8461 ataaaaaccg cggttttttt gttaactgtc tggattataa attgctcatt actaaagttc
     8521 aggcagcagg caaaaaggtt ttaagtgctt ccgcttttgc taatggagca cgccttgccg
     8581 ctaaagaaaa attaggaagt gaccaatgaa aaaagaatat tttgccataa ttaaattccc
     8641 tatgtttgtt ggcttatctc tgcttgccat cttgctgatt ttctttctga tggttttggg
     8701 cagctatcat catttagggc tttcaggatg gggaaatttc ttctataccc ttatctttat
     8761 attaattatt atattaattg ccagctggat tttaaccctg atcagtacgc actgtaaaat
     8821 aaaatcaacc tggctgagaa aatatatttc ctggatgcta gtgcgtgtat actatcctct
     8881 ggctatctgg atttctattc ccttcttcca gaaaagagct tcaatccagg aatcttttct
     8941 ctattttaat aacgaaatta tcgtaactaa ctatcaaggg atacactttt ctaatctatt
     9001 attgctttta ccacattgct tacaaaattc taactgtaaa atccgcatta gtagcgatat
     9061 cgtagattgt caggaatgcg gtggttgcga tattgccaaa atgaaatcta tcaccagaga
     9121 caatcatata aaagctgccg tagcttctgg tggttcttta gccagaaaat tggttaacga
     9181 tacaaaacca gatatgatta ttgcagttgc ctgtcatcga gatttaacag aaggagtgcg
     9241 tgatagctgg cgttatcctg tttactcagt tttaaatgaa cgccccaaag gaccctgttt
     9301 cgaaacaacc gttagctatt cagtgatcga ttttgccata aagaaattca tataaaagga
     9361 gaaaaccttg aaacgcatca atcttttcac cgttattctg atcctgttgc taaatgcctt
     9421 tatcacgatg gctttaatta ataaatatat ggataaatct gttgcaattg ataattcata
     9481 cacagaatat tcttccaaac caagtaaagc gtcagctgcc aatcgcatta attctataac
     9541 cgaagctgtt aaagcagtag aaccagcagt agttagtatc aatgtaataa aaacggaata
     9601 tgtgcgagct agaagcccct tcggctttgg tttttttgat ttctttggtt ttgcaccaat
     9661 gatgcgtcaa gtggaatcta tcggaagtgg cgttatttac gatcctaagg gctatataat
     9721 tacaaatgcc catgtggtta gtggtgctac tcaaattaaa gttattttgc ccgataaacg
     9781 cgaatatgaa ggtgtgatag caggaataga tgaaattcac gatgtggcaa aacttaaaat
     9841 taacggaaat aaccttcccg ttgccagatt aggaaattcc aacgatctaa ttatcggaga
     9901 atggagtatt gctgttggta atccctatgg ctttttaatg aacgattcca aacccagtgt
     9961 atcggttgga gttatttctg ccgtaagcag aaattttgct gctcggacag ataaacgcga
    10021 atataaatct atgattcaaa cagacgctgc tgttaatcct ggaaatagtg gtgggccgct
    10081 ggtgaatatt aatggcgaag ttgttggtat aaatactttt atcttttccg aaagtggagg
    10141 gaatataggc attggctttg cccttcctat caattctgtg aagaccattc tggccaaaat
    10201 atgagcaaaa aaatcccctt aataatgcta atattcatag caggaagttt gctttgtgcg
    10261 caaattacac atagctgtta tgatattcaa tataatcctg aaggaggttc aaattccccc
    10321 tacctggatc aagttgtaac tgttcaagga attgtaagcg ggatatcttt ttattcagga
    10381 accagcgtaa atagctatgg tttctttatt gctgatgcgg caggtggacc ctggagcggt
    10441 ttatatattt acaatcaaac ctatcatcct aatttgggag atttagttca gatcacagga
    10501 acagttaatg aatattatgg tttaacagaa atctctcaac ttacttcttt tcaggtttta
    10561 agccaggata atccacttcc agaacctgca gaaataaata gcggagccct caataatttg
    10621 agcagcggag aacagtggga aagtgtttta gtgaaagttc ttaatgtttc cgttgttacc
    10681 gaaccaaata actatcagga attttatgtt gatgatggca gcggtgtatg tcagatagat
    10741 gatcaatttt ttggttctgg tcacagctgg tctaatatta cattagggca agtatattct
    10801 gaaattacag gtattgtaga ttatgcctat aattactatg ctataaaccc ccgctttctt
    10861 acagaccttg ttttaagtgg gactacagcc acattactaa ttcctcatca agcagttagt
    10921 ttacatagct ctgttactgt tccagtaaat gctttgggca tagatttagc ttccagctat
    10981 caatcctatt ctttcaactt agctttcaat cctaatatta tatcttatca gtctgttagc
    11041 accgagggaa caatttctgc cccaggaact attaatgctt cttgcacaaa cggtttgctc
    11101 tctgttactt atcaaagcaa ttcagttatt agcggtcagg gtgtactttt aaatctgaac
    11161 ttttatgcaa ccaatacagg aacgactgtt ttagccctgg aaaatgtgtt tttcggcaac
    11221 gatagtatcc aaagcttaat tgatggcagt ttaaccgtta atagctgtta taattcgcct
    11281 ggtgacactt taacggttat ccagcgtcca cttcttaata ttccggaaat cgttattccg
    11341 ggagaaacat taacgataac ctgtttggca cctcaaaata caactaactg gaatgcctgg
    11401 ctgaaacata gtaataaacg cataaatatg ccgatttcaa acagccaatg gttaaataat
    11461 cctaacaggt gggaacttgt tacaactatt ccatctgttc cgatttttga attatacgat
    11521 ttggaggttt ctgccagtgg gggaattagc gatattacag aaaatgcgat tcaggtgatt
    11581 cctacgcgaa aaaataacta ttactttgtg catataacag atgcgcattt acctaaccgt
    11641 ttatactatc cgaatgcagg cttcgaaacg gattctactg cagtagaaga ttttagagca
    11701 gtgatggagg atatcaatct tattcatccg gaatttgtgc tttttacagg tgatttggtt
    11761 aatgaaggcg agctggaatc ttttgcgaat caatattggt atggctggac gcaaaaactg
    11821 ctttccgaat ttgatattcc agtttatgta acttctggaa atcatgatat cggcggttgg
    11881 aatcaaactc cacctccagc tggatctgcc agaagaaact ggtggaaata ttttggttgg
    11941 aactggttag atagtgaaga tcccaattgg ctatatcata ctcaggatta ttatttcacc
    12001 tacggaaaca caatttttat cgggctggaa agctatgata actatgatta ttggcgttta
    12061 aatatctatg gcacaaccag ttttaccgat caacaaattg cttggctaaa tagtactcta
    12121 tctctttttc caaactatac caaggtgcta tttcatcact acgattttca ggatgagctt
    12181 aatctttctg ctttaggaat tgatctggct ttgtgggggc atatccattc caataatggc
    12241 tcaatatcta atttccccta taatttagcc acgcgtagta tctgtgatgg aaatcgtgcc
    12301 tatcgaatgg tgcgagtgaa taataaccaa attataccct acaatactat ttatgctgga
    12361 gcaacaggaa ataacctctc tattcaatat ttcccttcca attatggagt agcggatagc
    12421 attagagcca ctattatcaa taatcaaccc ctaacctttg aaaatgccca aataaaattt
    12481 attatgcctt ctggaatgaa cggctataga gttcagggag gaattttgga acaggtagat
    12541 agaagcgggg ttaataatgt ttgctatgta cgcgtttcgc tttctgctaa tagcactacc
    12601 aatattagta ttgccgatag cggagtggaa aatatagacc ctctgcaggt gcctaagcca
    12661 ttacaaataa aaagcatcta ccccaatcct ttatccctgc aaggaaatat acaactttat
    12721 agtgaaaaat ccttgcctac cttaaccttg caactatata acctgaaagg acaactggca
    12781 tatcaacaaa ccttacaaaa cattaacaaa ggggaaaata ccctggcttt cacattgccc
    12841 gattccttag gaaacggaat atactttcta cgcttacctg aagataaaaa cacaacccaa
    12901 aaaatattaa ttcttaaaaa gtgagattat aatgcctgaa aaaagacctc aaactgctaa
    12961 acttctctac ataccttgga ttttaaaaca tctatcctat tatgtgaaat tgaataccca
    13021 tatccttaaa aaaggggtgc cactgaaaga aatcctgatc ttaaaatttc cgttctggtt
    13081 cagcgatgcc aaaattccac ccttgctttc tgtggatatc accgatgcct gcaatttaaa
    13141 atgtaactat tgtaataatc ccttcttccc acatccccgc acaatgatga gcaaagaagt
    13201 ttttgaagca ttaattgaac acctaaaacg ccaaaatgtt agccgcatca gaattagcgg
    13261 tggagaacca actctgcatc cacaattcga tacaatgctg aaagaaatgt cccactactg
    13321 taaatatcta tcaataatca ccaatggtca atggaaaaat aaagaaataa cagaaaaact
    13381 gctcgattgt ggacttagct ttatcgaact ttctatggac gccggaggaa aagaaatata
    13441 cgaaaaaaat cgccccggtg ccgattacga tctcttctta caaaacctga aagacctgta
    13501 tgaactgcgt aaccgaaaaa aaacagacct aataattaga attcgattaa tgctgcgtcc
    13561 ttccactcta tccttagaaa aagaagatag ccactattgg aaacaattca gcgattgcgt
    13621 tctgcctcaa catattctac aacatccgga aacaccaccc caagaagatg tttttatgca
    13681 ggattctatc ctccaaaatc aacttcctgt ctgtactgta acctatagag atctgcaaat
    13741 tagacccgat ggaaaagttc cactctgccc tgcaaaagga tgtgccataa atctcgaaga
    13801 ccgtattttt attggagata tccaaaagga cgatattctg gatctgtgga atgcaccaat
    13861 gctgaaacaa atgagaaacg ctcacaggaa aagacaggga gaaatattaa acctctgccg
    13921 taattgccat tatggatgaa tgcccactca tcggggaaca aaataacctc acgcagat
//
LOCUS       DAOSTH010000042        13801 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_4529_length_13801_cov_11.643969, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000042 DAOSTH010000000
VERSION     DAOSTH010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13801
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4529_length_13801_cov_11.643969"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="PLB65_05860"
     CDS             complement(<1..1879)
                     /locus_tag="PLB65_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012975.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HOQ78090.1"
                     /translation="MFDSSLGRQKISEKERLVDIPLYTFVAYFNKGILRFGFVTATKD
                     SYSEILTEDGTTTILSNTRFILTGKETLPNKNIVTFVSEVNEQAEQFEKTDFSFLADR
                     EYTLVEIVEELKLQSDVEIFALYKFLRAHPEHIKCKKHKYRLKTTEEIALFQIQQKEQ
                     EEEELFLQEVNAWFKGSELSIKNQHQLFCALPELQSDKKNKKLLELILANYPHHTPEQ
                     AILQFRKACGETPYYIDPIISNAGIPIGFSTLLNNEKLLPIQNSDPEETAFCIDDEST
                     KDFDDALSIAKDGSFWKVGIYVSSVSERLNFQGALFAEAKKRVSSLYTANAIIPLFPQ
                     QHSEKELCLLKNELRPVIALTLWLDSNFNIQRTEIARKKITISANYSYPEVDKHIAQE
                     PFATLFKFSKTLAEKREANSVPEEQRYYYYIVSTKKGLAVKCIDTQSPARKMVEELMI
                     VYNSSLAAYSVQENIPIIYRNINHIETLNTNLVISQAYLSTSPQFHPGIGVKAYMHTT
                     SPIRRYVDLINQTQILANLKGKTKPFSNEELENDIPRIEKTLLYLKETAHKSEHYWVL
                     EFLKQNYLNTPLDAYFRDGRNGFLRFELIPWGFVISAKCESYPLTDKLKIIIYKVDSD
                     QG"
     gene            complement(1854..1929)
                     /locus_tag="PLB65_05865"
     tRNA            complement(1854..1929)
                     /locus_tag="PLB65_05865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1894..1896),aa:Arg,seq:cct)
     gene            2071..3051
                     /locus_tag="PLB65_05870"
     CDS             2071..3051
                     /locus_tag="PLB65_05870"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOQ78091.1"
                     /translation="MKVAIVGATGEVGRMMITCLEESNLKVSELDLFASYRSAGTILY
                     YTDQPLTVQELKEDSLLTHYDYVLFSAGAGVAKNYAPIAAQTSELVIDNSSGFRQESD
                     IPLVVPQVNGELLIGYNGIVANPNCSTIQIILPLAVLDKLFNLNKLIVSTYQSVSGSG
                     HQGIETLMKQRKGTTDKGIYPQIIDLNVIPQIGGFLDSGYCQEEEKMHFESRKILSKW
                     DLAVCATTVRVPVVYGHSASVYAEFEDAVDIKAAETALQNAPGIVYYPNTYMTPLDLG
                     SSNDSHTCRLRLGTDDKSLAFWNVGHNVRLGAAANAVEILKTHAKYSGKL"
     gene            3051..4169
                     /locus_tag="PLB65_05875"
     CDS             3051..4169
                     /locus_tag="PLB65_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HOQ78092.1"
                     /translation="MIDIIELRHNLHRIPELAFREFKTQALLEDSILKILQNQPENIW
                     KLHYFKNSTGLLLEYTCAKTPYLLFRADMDGLPITENTGVPYTSEHPGFMHACGHDVH
                     LSVLMGLIQVVTETQPQRNLLFLFQPAEEGEGGAKRVLEEGLIQKFDIEAAFAFHIAS
                     ELPVGTVSAKEGIFFAIPQEFDVCFYGKAAHIAFPEKGINALKAGIMFMNVVEERLSE
                     LQKQEKMIFHIGKMSSGTIRNIIADKCILEGTHRSLSRKTCQKINNILKETAEFVEKQ
                     TNTKSEVKYLVSYDPVINNANLVKRLQEICSSKKIEFIPAETAMTGEDFGFFTTLYPS
                     LLFWLGSGCDYPLHSDKFLPRDESVEVGIRIMSAFLKR"
     assembly_gap    4311..4410
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4631..10087)
                     /locus_tag="PLB65_05880"
     CDS             complement(4631..10087)
                     /locus_tag="PLB65_05880"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ78093.1"
                     /translation="MKKIITLLLTILAAGSMFANAYDFNQPPNLNSAFALGARNDSNM
                     RVNFVLPEYSVQEETYGETVYHKIMLPLSGTLMEPGMPELPSVCTYIAIPHRGGVNIE
                     VLSTQQTVIPNFLPYPVQQGNNLESPKGFVLNSDYYNGSGSNYPESLIDVSAPFILRD
                     FRIINIKINPFSYNAQTGELTVNQNIQFRLNFTNEPGINELLCEPENISASFDNIYNS
                     MILNYADYRDAVVAQTPPRYLIIYGTNTDPNFTDALNGYVLWKKQKGADVMVASTATS
                     EAGNSTTSIKNYIQNKYNNPATRPDFVVLIGDTSGSYTIPAFTESGGATDYNYTFLAG
                     SDQLGDCFIGRISVENLSQFLVVLNKIYLYERDINLDTASWLNRMMLSGDNSPSGIST
                     MYIHKYIKEMSSFVNPNYSYVEDYGSSPNYTVINQALNQGVGFYSFRGYIDWVPPNES
                     SLFNGYKLFHTVNITCGSNNYDSAGEVEAFVRYGTTAAPKGAVTGIGMCTSSTHTTFN
                     NAVHGGIFSGIFASDMRTMGEALLNGRLYLNQLFGVSSPANAVKFAAWCNLMGDPTME
                     VFTGIPNHFNVTCESSIPLGLSLYDVAVHDSVGNPVEGAAVVLNMGGMIIARGYTGID
                     GTVILVLPSNMIAGEATLTISTHNFKPKQLIIPVINIPTLVPGTIAIDDDDISPSQGN
                     GNGIAGSGETIELLFGLVNTGTDAISGISGTISSNSPYITIVNPSINYSDIPGGQLGI
                     NTTPIVINIAQNAPHQTMLRLYLDLTDSNSTNYHISEYIQVEAPFMEFVSYLVIDENN
                     QHLDPGETAEFSITVKNVGTVPVENVMATLYTLNDLVGVVDANADFGTLPTGISVTCG
                     VDRFSLIARLQVLPGMLIPMKLKLYNNDGFEQYIPFTFTVGLVTQHDPLGPDTYGYVI
                     YDWTDTAYEFVATYDWHGISPSEGGLGTAVAISDGHSGSNEGDQVGSDALEIVALPFP
                     FQFYGQLYDQITICSNGFIAMGATENAEFRNYRLPGPMGPNPMIAPFWDDLATGSGSG
                     IYTWFDRYSHCFIIEWHNMINGKDGSSVENFQCILYDQAAYPTSLGDGPIKFQYHTFN
                     NVDSQSGNRHGNYCTIGIEDHTGKVGLEYTFNNTYPTAAAPLSNGKALFITNIPVYHE
                     AAHLILEATYVNDPNMNGICEPGELIDLGVKIKNTGNLIAENIVGHLSTTSEYVTINN
                     AIASYYPIEPGFYGVNRQPYTFTISGSCPNGNVIYFNLLLTSGDLEWSRNFSFQVSAS
                     SLNYHSHFVDDHLSDFNGTVSLGESFNLVINLENTAAVDATNIQATLSSTIPELVIEN
                     PIVLLDKIGPNDIYQTTYTIDTSMLDASITQIPFLFNATAGNGSATSTAFNIQYNNPI
                     VYEDFDLNNGIFISETGWTWGIPTQVSPFSGTKVWATNLSGSYPDLVTYNLYTPQYLL
                     AENSQLQFMHNYICEYGYDGGNVAISTNNGNSWSLIYPTTNYNGNSLNGLSGDQGWTG
                     NSGGWQTATFNLSQYAGQTVIFRFRFGSDGSTSGSGWFIDDFKISGVDLKTGYLYGII
                     YPTSGLDPSLATLKSNLRLTSHPDSEGNFILLLPYGIHSVTASMPYHQSSSITSVIIS
                     SSNPDHYTEFTLIDLPRPQNIYYSGNNETGELYIYWTPPEDTVLPVVGYKVYRKFNTD
                     QFYLVQESEETTYHEVLSLEGTYVYYIVVKFFNVDGTPSQFINIPFPFEDVSDNEIPS
                     LVTKLNNNYPNPFNPSTNISFTLSKPGRTTLNIYNMKGQLVNHLVNEDMNPGIHNVVW
                     NGKDENGRNVSSGVYLYRLESGTYQATRKMLLMK"
     gene            complement(10153..11574)
                     /gene="glnA"
                     /locus_tag="PLB65_05885"
     CDS             complement(10153..11574)
                     /gene="glnA"
                     /locus_tag="PLB65_05885"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOQ78094.1"
                     /translation="MELKKLQQMIEENAVKAVDLKYCGLDGKWYHITFPARNIAEVME
                     SGIPFDGSSIPGMTSVESGDMVLMPDASTAQLDAFNATPTLRMICSICDAETRIGVNK
                     DPRSVALRAHKYLLSTGIADMSTWIPELEFHLFDTVQYYSDAFSSGYNLTSSECKDAL
                     PEDNEDLDALALQGVKGYHMDTPFDQFYEIRQKIVELIEEQDIKVRYHHHEVGLSAQE
                     EIETELLAFPKICDDVMVMKDIIRRTALQYGLTATFMPKPVYGHAGNGMHFHIMLHKN
                     GKNLFYKKGGYADLSDEAIWFIGGVLTHGRALVAFTNPSTNSYKRLLPGFEAPVKLFY
                     GLANRSAAIRIPKYVNTPETKRFEFRTGDGTCNPYFAMSAILMAGLDGIKRQIDPGKY
                     NFGPFDDNVFAWSEEKKATLLSIPANLEEAMQALKVDHQFLLEGDVFNKELIESHINL
                     KLKENDAVSARPHPYEMMLYYNL"
     gene            complement(11834..12478)
                     /locus_tag="PLB65_05890"
     CDS             complement(11834..12478)
                     /locus_tag="PLB65_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOQ78095.1"
                     /translation="MNNSNVISLLLNVVIIALVFYSIIIHEFSHSLAAFLLGDDSAKR
                     AGRLTLNPLKHIDWFGTVILPLFLYFTCGFIYGYAKPVPFNPYNFKNFKTDSGLTGLA
                     GPLSNFLVAIVFALIYHLFGFSFIVQYICTYVIYLNLLLAFFNLIPIPPLDGSKVIGI
                     FLTDQAYYKWTMQERKGMIYLFAFIIISSLLGWNIIGKIIVPPVNFIMNLMGVH"
     gene            complement(12475..13677)
                     /gene="tyrS"
                     /locus_tag="PLB65_05895"
     CDS             complement(12475..13677)
                     /gene="tyrS"
                     /locus_tag="PLB65_05895"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOQ78096.1"
                     /translation="MAFEQEMKLISRGVEEIITREELEAKFAKSVKTGRPLRVKYGID
                     PTGCDVHLGHLVPIRKMRQFQDLGHIGVIIIGDFTAQIGDPTGRDESRPPLTSEQVIK
                     NSEKFMEQLYTVLKPEKTEVRYQSEWFGKMGMSEVIKLMSKFTLAQFMAHETFRQRYE
                     QGLSLGMHEILYPVLQGYDSVAIESDIELGATEQKFNILCGRDMQRHFGMEQQVAILS
                     PILIGTDGVQKMGKSLNNYIAIFDSPKDKYGKVMSIPDDLILNYYCYATTVPEEEIDK
                     IKLELESGINPMLIKKRLAREIVSLYHSAAETKEAEEAFEKQFSMREIPDDIPEFIIG
                     ESEYKLAKLLVDSKICATNGEAKRLIQGGAVSVNGNKITAFDAIISLQEGDVIKAGKR
                     NYIKIKKI"
BASE COUNT         4260 a         2543 c         2566 g         4332 t
ORIGIN      
        1 atccttgatc agaatccact ttataaatta taattttcaa tttatctgtt aatgggtagc
       61 tttcgcattt tgcagatata acaaaacccc aggggataag ctcaaaccgc aaaaagccat
      121 tgcgtccatc tctgaaataa gcatccagtg gggtgtttag gtagttttgt tttaaaaatt
      181 ccagcaccca ataatgttca ctcttatgag ctgtttcttt taagtagagc agggtttttt
      241 cgatgcgggg gatgtcattt tccagttctt cattgctaaa aggttttgtt ttccctttca
      301 ggtttgctaa gatttgggtt tgatttatta aatctacata ccggcggatt ggggaagttg
      361 tatgcatata ggctttaacg ccgattccgg gatgaaattg tggggaagtg gaaagataag
      421 cctggctgat aacaagatta gtattcaggg tttcaatgtg atttatatta cgatagataa
      481 tagggatatt ttcttgaact gagtaagctg ccaaagagga attatataca atcataagtt
      541 cttcaaccat ttttctggct ggactttgag tatcgataca tttaactgcc aagccctttt
      601 tagtggatac aatataatag taatatcgtt gttcttcggg aactgagttt gcttctcttt
      661 tttctgctaa ggttttgcta aatttgaata gggttgcaaa gggttcttgt gcaatatgct
      721 tatctacttc aggataacta taattagcag agatagttat cttctttctg gctatttcgg
      781 tgcgttggat attaaagtta ctatccagcc ataaagttaa agctattaca ggtcttagtt
      841 cattttttag caaacagagt tccttttctg aatgctgttg aggaaataaa gggataatgg
      901 cgttcgctgt atataatgaa gaaactctct tttttgcttc tgcgaacaag gctccttgaa
      961 aatttagccg ttctgaaaca gagcttacat aaattcctac cttccagaaa gagccatctt
     1021 tggcaatact gagggcatcg tcaaaatctt tggtggattc atcatcaatg caaaaagcag
     1081 tttcttccgg atcagaattt tggatcggga gtaatttttc attatttaaa agggttgaaa
     1141 agccgatggg gatccctgca ttagaaataa taggatctat gtaatatgga gtttcaccgc
     1201 aagctttacg aaattgcaga attgcttgtt ctggtgtatg atgaggataa ttggccaaaa
     1261 tcaattctag taacttttta ttttttttat ccgattgcag ttccggtaag gcacaaaaaa
     1321 gctgatgttg atttttgata ctaagttcag aacccttgaa ccaggcatta acttcctgca
     1381 aaaaaagctc ttcttcttcc tgttcttttt gttgaatttg gaatagagct atctcttctg
     1441 ttgttttcaa acgatattta tgctttttac atttgatatg ttcggggtgt gctcgtagaa
     1501 atttatatag ggcaaaaatc tctacatcag attgcagttt aagttcttcc actatttcca
     1561 ctaaagtata ttctcggtct gctaagaagc tgaaatcggt cttttcaaac tgctcagctt
     1621 gttcatttac ttcggataca aaggtaacta tgtttttgtt gggaagtgtt tctttacctg
     1681 ttaaaataaa acgcgtatta ctcaaaattg tagttgttcc atcttctgta agaatttcgg
     1741 aataactatc ttttgtagca gttacaaagc caaacctcag aattccctta ttaaaatagg
     1801 caacaaaagt ataaagtgga atatctacca atctttcttt ctcactaatt ttctggcgcc
     1861 ccagagatga gtcgaacatc cgaccaacgg tttaggaaac cgctgctcta tccactgagc
     1921 tactggggcg catttattgt tcaccaatct gacaagagcc cttttttgtc aagtttacat
     1981 tgacaagcta tcccttcaca aaaaaagtga atgcgtttta gaatcatacg attaagatac
     2041 ataaaataag gaaaaacagg taaacaaaaa atgaaagtag caatcgttgg cgcaacgggt
     2101 gaagttggca gaatgatgat tacctgcctg gaagaaagca atttaaaggt gtcagaatta
     2161 gatttatttg cttcatatcg ctctgcaggt acaattttat attacactga tcaaccactt
     2221 acagttcaag aactgaaaga agattctctg ctaacacatt atgactatgt tcttttttct
     2281 gcaggtgcag gagttgctaa aaactatgct ccgattgcag ctcagacctc tgaattggta
     2341 atagataact cttctggctt cagacaggaa tcagatattc ctctggttgt cccacaagta
     2401 aatggagaat tgcttattgg ttataatggt attgtggcca atcctaattg ttctacaatt
     2461 cagattattt tgcccttggc ggtgttggat aagctcttta atttaaataa gttaattgtt
     2521 tctacttatc aatcagtttc tggaagcgga catcagggaa ttgaaacttt gatgaaacaa
     2581 cgcaaaggca caactgataa aggaatatat ccgcaaataa tagatttgaa tgtaattcca
     2641 caaattggtg gttttctgga tagtggctat tgccaagaag aagaaaaaat gcattttgaa
     2701 agccgtaaga tactatctaa atgggattta gctgtatgtg ctactactgt tcgggtgcca
     2761 gttgtttatg gacacagtgc ttctgtttat gccgaatttg aagatgcagt agatataaag
     2821 gctgcagaaa cagctttaca gaatgcacct ggaattgtct attatccaaa tacttatatg
     2881 acgcctttgg atttgggttc ttccaatgat tcacacactt gccgtttacg gttagggacg
     2941 gatgataagt ccttagcttt ttggaatgtg ggtcataatg tgcgtttggg agctgctgct
     3001 aatgctgtgg aaattctaaa aacgcatgca aaatattcgg gtaagctttg atgattgata
     3061 taattgagct gcgtcataac ttacatcgga ttcctgagct agcatttaga gaattcaaaa
     3121 cacaagcgct gctggaagat agtattttaa agattctgca aaatcaaccg gaaaatatct
     3181 ggaaattgca ttattttaaa aacagcactg gcttgctttt ggaatacact tgtgcaaaaa
     3241 caccctatct attattcaga gcggatatgg atggcttacc gattacagaa aatacaggtg
     3301 taccttatac ttcagaacat cctggtttta tgcatgcttg tgggcatgat gttcatttaa
     3361 gtgttttgat ggggctaatt caagttgtaa cagaaaccca gcctcagcga aatttgctct
     3421 ttcttttcca gccagcagaa gaaggagaag gaggagcaaa aagagttttg gaagaaggcc
     3481 taattcaaaa gtttgatatt gaagctgcct ttgcttttca tatagcaagt gaattgcccg
     3541 ttggaactgt ttcggctaaa gaaggtatct tttttgccat tcctcaggaa tttgatgttt
     3601 gtttttatgg caaagcagct catatcgctt ttccagaaaa agggataaat gctctaaaag
     3661 caggaattat gtttatgaat gtagtggaag aacgcttatc tgaacttcag aaacaggaaa
     3721 aaatgatctt ccatatcggc aaaatgagct cgggaacaat tcgtaatatt attgccgata
     3781 agtgtattct ggaaggaaca catcgctcgc tttccagaaa aacctgccaa aaaattaata
     3841 acatcctaaa agaaactgca gaatttgtag aaaaacaaac taatacaaag agtgaagtga
     3901 aatacctcgt ctcctacgat ccggtaatta ataacgcaaa tttagttaaa cgcctccagg
     3961 aaatatgcag tagcaaaaaa attgaattta tccctgcaga aactgctatg acaggtgaag
     4021 attttggctt ttttactact ctttatccct cattgctttt ctggctggga agcggatgcg
     4081 attatccctt acattcggat aaatttttac ctcgagatga aagcgtagaa gttggaataa
     4141 ggattatgag cgctttcttg aaaagataat acctctctca cagattgaac tatcgaaaca
     4201 aggatttaga ggattttaga tatttagcca ccgagatcac cgaactacga actttgaact
     4261 acgaactttg aactacgaac tttgaactac gaactttgaa ctacgaactt nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aactttgaac tacgaacttt gaactacgaa
     4441 ctttgaacta cgaactttga actacaaact ttgaactaag tacttttgct tttccaaaaa
     4501 cagaagtcct cactgctaaa caacctacag gataggtaag ggttgtttga acagtgagga
     4561 cttctaactt tcgctattga ttgtttctaa cttatattat actcaatttc ctttgttagc
     4621 atatactagg ttatttcatc agcagcattt ttctggttgc ttgataggtt ccactttcca
     4681 gacgatataa atacactccg gaggaaacat tgcgtccatt ttcatctttt ccattccata
     4741 caacattatg tattccagga ttcatatcct cgtttaccag gtggttaaca agttgtcctt
     4801 tcatattata gatgttcaaa gttgttcttc ctggcttgga caaagtgaag gatatattag
     4861 tggaaggatt aaacggatta ggatagttat tattcaattt agttacaagg gatggtattt
     4921 cattatctga aacatcttca aaggggaagg gtatatttat aaattggctg ggagttccat
     4981 ccacattgaa gaacttcact acaatgtaat atacataagt tccttctaaa gaaagaactt
     5041 catgataggt ggtttcttca gattcttgaa ccagatagaa ctgatccgta ttgaatttac
     5101 gatatacttt atagccaact acgggtagaa ctgtatcctc tggaggtgtc caatagatat
     5161 aaagttcccc tgtttcgtta tttcctgaat aatatatgtt ttgtgggcga ggtaaatcaa
     5221 tcaaggtaaa ttcagtatag tggtcaggat tactggaaga gatgatgact gaagtgatcg
     5281 aagatgattg atgatagggc atacttgccg ttacagaatg aatgccataa ggtaagagaa
     5341 ggataaaatt accttcagaa tcgggatgac tagtaaggcg aaggttagat tttagggttg
     5401 ccagagaagg atcaagccca gaagttggat atataattcc atataaatag cctgttttga
     5461 gatctacgcc gctaatctta aaatcatcaa taaaccatcc agaaccactt gttgatccat
     5521 cggagccaaa acggaaacgg aatattacgg tttgtcctgc atactgtgaa agattaaatg
     5581 ttgctgtctg ccaaccacct gaatttcctg tccaaccttg atctccactt agtccattta
     5641 ggctattacc gttataattc gtagtgggat aaattaaact ccaagaattc ccattattgg
     5701 tagaaatagc tacattacca ccatcatatc catattcgca aatgtaatta tgcataaatt
     5761 gcagctgact gttttctgcc aacagatatt gtggtgtgta aagattgtag gttactaaat
     5821 caggatagct tccgcttaaa ttggtagccc aaacttttgt tccggaaaag ggggatacct
     5881 gtgttggaat tccccaagtc catcctgtct cagatataaa gatgccatta ttcaaatcaa
     5941 aatcctcata cacaatagga ttattatact gaatattgaa ggctgtgctt gtagcacttc
     6001 catttcctgc tgtagcatta aagagaaatg gaatctgggt aattgaagca tccagcatag
     6061 aagtatctat tgtatatgta gtttgataga tatcattggg accaatctta tccagtaaaa
     6121 caataggatt ttcaataacc aactctggaa tagtgctgct taaagttgct tgaatgtttg
     6181 tagcatctac tgcagcagta ttttccagat taattactaa gttgaaagat tctccaaggc
     6241 taacagtgcc attaaaatca ctcaagtgat catccacaaa atgggaatga taatttaaag
     6301 aggaagcaga gacctgaaaa ctgaaattac ggctccattc taaatcgcca gaagtaagca
     6361 agagattgaa gtatataaca ttaccattgg gacaggaacc agatattgtg aaggtgtaag
     6421 gttgtctatt tacaccataa aatccaggtt cgataggata ataactcgca atggcattat
     6481 tgatagtaac atattcactt gtggtgctaa gatgtccaac tatgttttct gcaataaggt
     6541 taccagtatt tttaattttc acacccagat ctattaattc tcccggctcg caaattccat
     6601 tcatattggg gtcgttcaca taagttgctt ccagaattaa atgagcagct tcatgataga
     6661 cgggaatatt agtaataaat agtgctttac cattacttaa gggtgcagca gctgtgggat
     6721 aggtattatt gaaggtatat tctaaaccaa ctttccctgt atgatcttca ataccgatag
     6781 tgcaatagtt tccatgtctg tttccggatt gagaatctac attgttaaag gtatgatact
     6841 ggaattttat tggtccatca cccaaacttg taggatatgc agcttggtca tataaaatac
     6901 attggaagtt ttctacagaa ctaccgtctt ttccatttat catattatgc cattctatta
     6961 tgaagcaatg gctatatcta tcaaaccagg tataaattcc actgcctgaa ccagttgcca
     7021 aatcatccca aaatggggca atcataggat tgggccccat aggaccaggt aggcggtaat
     7081 ttctaaattc agcattctca gtagctccca tagcaataaa accattggaa cagatagtta
     7141 tttgatcgta aagctgacca tagaactgga aggggaaagg caaagccaca atttctaagg
     7201 catcagagcc aacttggtcg ccttcattac taccgctgtg accatctgat atagctactg
     7261 cagttcccaa acctccttca gaaggagaaa ttccatgcca atcataggta gctacaaatt
     7321 cgtaggcggt atcagtccaa tcgtatatta cataaccata agtatcagga ccaagaggat
     7381 cgtgttgagt aactaggcct actgtaaaag tgaatgggat atattgttca aatccatcat
     7441 tattatatag ctttaacttc atcgggataa gcatccctgg taaaacctgc aaacgagcaa
     7501 taagagaaaa acgatctact ccacaggtta ctgaaattcc tgttggcaga gttccaaaat
     7561 cagcattagc gtctacaaca cccactaaat cgtttaaagt gtataaagta gccataacat
     7621 tctctacggg aacggttcca acattcttaa ctgtaatact gaattcagcg gtttctcctg
     7681 ggtcaagatg ttgattattt tcgtctataa caaggtagga aacaaattcc ataaaaggag
     7741 cttccacttg aatatattct gagatgtgat aattagtaga attggaatcg gtaagatcca
     7801 ggtatagtcg taacattgtt tggtggggag cattttgggc aatattaatt actataggag
     7861 tagtattgat tcctaattgt cccccaggaa tatcagaata gttaattgat ggattaacaa
     7921 tagtaatata ggggctgtta gaactgatag ttccgctaat tccactaata gcatcagttc
     7981 ctgtattcac taagccaaaa aggagttcga tagtttcacc gctaccggca attccatttc
     8041 cattaccctg tgagggagat atatcatcat catcgatagc tatagttcct ggaaccaaag
     8101 tagggatatt tatcacaggg attataagct gcttgggttt gaaattatgc gtgctaattg
     8161 ttaaagttgc ctcgccggca atcatattgg aggggagcac taaaatgacg gttccatcta
     8221 tgcctgtata accgcgagcg ataatcatac cccccatatt taaaactaca gcagctcctt
     8281 ctacaggatt tcccacacta tcatgaactg ctacatcgta taaacttaat cccaaaggaa
     8341 ttgaactttc acaggttaca ttaaagtgat ttggaatacc agtaaaaact tccattgtag
     8401 gatcacccat taaattacac caggcggcaa atttaactgc atttgctgga gaggaaactc
     8461 caaatagttg attcaaatag agccgcccat ttaataatgc ttcacccata gtgcgcatat
     8521 cagaggcaaa tattccggaa aaaattccgc cgtgtacagc attattaaaa gtagtatgag
     8581 tgctggaagt acacattcca attccagtta cggcaccctt aggagctgca gttgttccgt
     8641 atctaacaaa agcttctact tctcctgctg aatcataatt attcgagccg caagttatat
     8701 ttacagtatg aaaaagttta taaccattaa aaagagagga ttcattggga ggaacccaat
     8761 ctatataacc cctgaaacta tagaaaccaa ctccttgatt aagagcctga ttgattaccg
     8821 tataattcgg agaagaacca taatcttcta cataactata attgggattt acgaatgagc
     8881 tcatttcctt tatatattta tgaatataca ttgtagaaat gccagaagga gaattatctc
     8941 cagaaagcat cattctattc agccagctgg ctgtatccag atttatatct ctttcgtaaa
     9001 ggtatatttt atttagcacc accagaaact gagagagatt ttctaccgaa atacggccta
     9061 taaaacaatc tcccaattga tcgcttcctg ctaaaaaagt ataattatag tctgttgctc
     9121 cacccgattc cgtgaaagca ggaattgtat agcttcctga tgtatcacca attaagacta
     9181 caaaatcagg acgggttgca ggattgttat atttattttg gatatagttt ttaatggagg
     9241 tagttgagtt tccagcttca ctggtagctg tgctggccac cattacatct gcacctttct
     9301 gttttttcca tagaacataa ccatttaagg catctgtaaa gttcggatcg gtatttgtgc
     9361 cgtaaataat caaataacgg ggtggggttt gtgctactac agcatcacgg taatcagcat
     9421 agtttaaaat catagagttg tatatgttat caaagcttgc tgatatattt tccggttcac
     9481 aaagtagttc atttattccc ggttcatttg taaaattaag gcggaattgg atattctgat
     9541 taacggtaag ttcaccagtt tgagcattat aagagaaggg atttatttta atattaatta
     9601 ttcggaagtc ccttaaaata aaaggtgcgc taacatcaat caagctttcg ggatagttac
     9661 ttccactccc attgtaataa tcactattta aaacgaatcc tttagggctt tccaaattat
     9721 tgccctgctg tacaggataa ggtaaaaaat tgggaataac ggtttgctgc gtagataata
     9781 cctcaatatt aacccctcct ctatgaggaa tagcaatata ggtacaaact gaaggtaatt
     9841 ctggcatacc tggttccata agagttccgg aaagaggtag cattatttta tgataaaccg
     9901 tctctccata agtttcttcc tgaacagaat attccggaag gacaaaattt acccgcatat
     9961 tggagtcgtt tctggctcct aaggcaaagg cagaatttaa gtttggaggt tgattaaaat
    10021 cgtaagcatt ggcaaacatt gaacctgcag ccaagattgt tagcaataag gtgataattt
    10081 tcttcatcgg acttccttta gataaattag gatctttgct ctataataaa agcaataatt
    10141 attccaagtt cattacagat tataatataa catcatttca tatggatgag ggcgagctga
    10201 aactgcatca ttttctttca gcttcaaatt gatatggctt tctattagtt ctttgttaaa
    10261 aacatctcct tccagcagga attgatgatc tactttcaag gcttgcattg cttcttccag
    10321 attggctgga attgaaagta gagtagcttt tttctcttcg ctccaggcaa agacattatc
    10381 atcaaacggt ccaaaattat acttaccagg gtcaatttgt cttttgatgc catccagtcc
    10441 agccatcaaa attgcactca ttgcaaaata gggattgcag gttccatctc cggttctaaa
    10501 ttcaaagcgt tttgtttcag gagtattaac atattttggg atgcggatag ccgcactgcg
    10561 attagcgagg ccataaaaca gctttacggg agcttcaaaa ccgggcagca atcttttata
    10621 actatttgtg gaaggattag taaaagccac taaggctcgt ccatgtgtta acacccctcc
    10681 aataaaccag atagcttcat ccgagaggtc tgcatagcca ccctttttgt aaaaaaggtt
    10741 cttaccattt ttatgtagca ttatatgaaa atgcattcca ttaccagcat ggccatatac
    10801 cggtttaggc ataaaagtag ctgttagccc atattgtaaa gcagttctac gaattatatc
    10861 tttcatcacc attacatcat cacatatttt cggaaaggca agtagttctg tttcaatttc
    10921 ttcttgagca gataggccta cttcatgatg atgatagcga actttgatat cttgttcttc
    10981 aatcagttcc acaattttct ggcggatttc gtaaaactga tcaaaaggag tatccatatg
    11041 ataaccttta accccttgca gggcaagagc atcgaggtct tcattatctt ccggaagagc
    11101 atctttacat tcactggaag taagattata gccagaagaa aaagcatcgg aataatattg
    11161 aaccgtatca aataaatgaa attccagttc tggaatccag gtagacatat ctgcaatacc
    11221 cgtagaaagt aaatatttat gcgctctgag agcaacactg cgagggtctt tatttacccc
    11281 tattctggtt tctgcatcgc aaattgaaca aatcattctg agagttggag ttgcattgaa
    11341 tgcatccaac tgcgctgttg aagcatccgg cattaaaacc atatctccac tttccacact
    11401 tgtcattcct ggtatggaag aaccatcaaa aggtatacca ctttccatta cttcagcaat
    11461 attacgggca gggaaagtga tatgatacca tttaccatcc aaaccgcaat atttaagatc
    11521 tactgctttc acggcatttt cttcgatcat ttgttgtaat ttcttcagtt ccattttgtt
    11581 tctcctatat cttttttttt cttatttagg gggaaacgat gttattcatc gttcacctct
    11641 aaacacgatt agcttttgta agagcatcgg ctgctacaca atatcactta aacaaatacg
    11701 gacattatga gccctttgta ccttcccaag atataagctg ctaataagtt cgatggacac
    11761 aagtttctcc taacttacag ggatattaat ctttttttat ctgggaatac taaataatca
    11821 gcacgcgaca tttttagtgc acacccataa ggttcataat aaaattcaca ggcggcacta
    11881 tgatttttcc aataatattc catcccaaca agctgctaat tatgataaag gcaaaaagat
    11941 agatcatccc ttttcgttct tgcattgtcc atttataata ggcttgatcc gttagaaaaa
    12001 tacctatcac ttttgatcca tccaatggcg gaatagggat cagattaaaa aaggcaagca
    12061 gcagattgag atatatcaca taagtgcata tatattgaac tataaaggag aaaccgaata
    12121 gatgatagat taaggcaaag actatggcca ctaaaaaatt ggatagcgga cctgccaatc
    12181 ctgttaaccc tgaatcagtt ttaaagttct taaaattata aggattaaat ggaaccggct
    12241 ttgcataacc gtatataaaa ccgcaggtaa aatataaaaa caacggcagg ataacggttc
    12301 caaaccagtc tatgtgtttt aaaggattca gggttaagcg tccagctctt tttgctgaat
    12361 catctcctaa aagaaaagca gccagagagt gactaaattc atgaatgata atgctgtaaa
    12421 agaccagagc gattataaca acattaagta gtagtgatat tacattcgag ttattcatat
    12481 cttctttatt tttatgtaat tgcgttttcc tgctttgata acatcaccct cttgaagaga
    12541 tataatagca tcaaaagctg ttatcttatt tccattcacc gaaacagctc caccttgaat
    12601 taggcgtttg gcttcgccgt tggtagcgca aattttactg tccaccagaa gctttgccag
    12661 tttatattcg ctttcgccta ttataaattc gggaatgtca tcgggaattt cacgcattga
    12721 aaattgtttt tcaaatgctt cttctgcttc ttttgtttca gccgcgctat ggtataggga
    12781 tacaatttcc cgagcaaggc gtttcttaat tagcattgga ttaataccag attccaattc
    12841 aagtttaatt ttatctattt cctcttctgg gacagtagta gcatagcaat aataatttag
    12901 aataaggtca tcggggatag acataacttt gccgtattta tctttggggg aatcaaatat
    12961 agcaatatag ttattaagtg attttcccat tttctgcaca ccatcggtgc cgattaaaat
    13021 tggtgacaaa atagctacct gttgttccat tccaaaatga cgctgcatat ctctaccgca
    13081 gagaatattg aatttttgtt ccgtagctcc aagttctata tcactttcaa tggcaactga
    13141 atcgtaaccc tgcaaaactg gatacagaat ttcatgcatt cccaaggata aaccttgttc
    13201 atagcgctgg cgaaatgttt cgtgagccat aaattgagct aaagtaaatt tactcattag
    13261 tttaataacc tcgctcattc ccattttacc aaaccactca ctttgataac ggacttctgt
    13321 tttttccggt tttagcaccg tatatagctg ctccataaac ttttcactgt tttttatcac
    13381 ttgttcggaa gttaaaggag gacggctttc atctcgacct gtaggatcac ctatttgagc
    13441 agtaaaatca ccaattatta ttactccgat atgacccaaa tcttgaaact gcctcatttt
    13501 ccggatagga accaagtgtc ctaaatgaac atcacaaccg gtaggatcaa taccatattt
    13561 tactctgagt ggtcttcctg tctttacaga tttagcgaat tttgcttcca gctcttccct
    13621 ggtgataatt tcttccacac ccctgctgat taacttcatt tcttgttcaa aagccattga
    13681 atcatcctat tttagtaatt atattgtctc agggcaaaaa tagtcaacgc atatatttgt
    13741 caagggtaaa gggaattttc ttgatggatt aaatgaatag ctgaaacatt taagaatatt
    13801 t
//
LOCUS       DAOSTH010000043        13630 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_4595_length_13630_cov_11.515064, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000043 DAOSTH010000000
VERSION     DAOSTH010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13630
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4595_length_13630_cov_11.515064"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="PLB65_05900"
     CDS             <1..651
                     /locus_tag="PLB65_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78097.1"
                     /translation="KVFSRFQIDIKYLTDIPEYEIWRWEHHFPKYLISARDYKTGAIY
                     LAYTQEKSSMATALFVDYLLSSLQYYGIDPQSIVIQTDNGKEFTNPTDRKVTLFEKLV
                     KEKYQATHFLIPYRRPTWNSDVESFHNLIEEEFFLSEKPNSEIDLLAKTFAYQIWFNS
                     FRNNRGRNNQNPEQIFQSELNTMKPLPNIPPILVDKYIQLIAKDNEVGYFLPLPPS"
     gene            693..1154
                     /locus_tag="PLB65_05905"
     CDS             693..1154
                     /locus_tag="PLB65_05905"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ78098.1"
                     /translation="MIKIDMITRNRKQFLDLLLLADEQEDMIDRYLEKGDMFALYDDD
                     LKSICVVVPIDSDTCELKNIATYEKYQGRGYGRTLVSYICDYYKKDYKTMLVGTGETP
                     ASLSFYEGCGFQRSHRLKNFMIDNYDHPIFVGDIQLIDMIYLKKDLGGGDR"
     gene            1581..2630
                     /gene="hrcA"
                     /locus_tag="PLB65_05910"
     CDS             1581..2630
                     /gene="hrcA"
                     /locus_tag="PLB65_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOQ78099.1"
                     /translation="MKKNQLRLNETLKSLVDEYILSCEPVSSRILNEKYLQKVSSATL
                     RLDLLKLEKMNLISQPHTSSGRVPTIKGYREYLKLIEPDIEKTRYEGMDLLRELLVRN
                     YKDTPRALHYIMENLAKETDQLSFVAEPEVSNGYLAKLEVFSIGENKLIFVMSLDSGL
                     DKTVIMKCDNELTESQLKKLVRYLNDELVGLQIYDIANRVLVEMQENSDNGVISWFLV
                     ELHKAFMEISDFFIHYDGSINFLEQKEFDTKENILKFMSLLQNQDFLLNMMRKHQSNK
                     ETNVVLGDDFTKSNWLDFALIYSKYEMFGIPGYLGVLSPIRTEYRKLIPLIRDVAKTI
                     TNTTRRGMIVPQEKI"
     gene            2630..3184
                     /gene="grpE"
                     /locus_tag="PLB65_05915"
     CDS             2630..3184
                     /gene="grpE"
                     /locus_tag="PLB65_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOQ78100.1"
                     /translation="MNKKTQKEPENNKDIQVEEVIEEQKTDKIQELEQEIAEWKDKYL
                     RCMAEFENFRKRSIAEKADWIRLATQKFALEICDVLDNFERAILQGSEEEKSTPFGKG
                     VLMIEQQLRKALEREGVRKIEALGQPFNPEFHDALAHIPSDLENNTVAAIIQNGYTMH
                     DKVLRPVRVAVSNGSKINNESQEE"
     gene            3253..5217
                     /gene="dnaK"
                     /locus_tag="PLB65_05920"
     CDS             3253..5217
                     /gene="dnaK"
                     /locus_tag="PLB65_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOQ78101.1"
                     /translation="MGKIIGIDLGTTNSCVAVVEGGKPVVITNAEGSRTTPSVVGFTK
                     DGQRLVGQLAKRQAITNPQNTVFSIKRFMGRGYDEVTEEIKQVPFKVIRGLKNQAAVH
                     IDMENKDYTPQEISAMILTKMKETAEAYLGQKVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLDVKRIINEPTAAALAYGMENKKEQKVAVYDLGGGTFDISILDISEGVVEVLSTNG
                     DTHLGGDDFDKRVIDWILEQFKKQEGMDLSRDPMAMQRLREAAEKAKIELSGTLTTNI
                     NLPFITADATGPKHLNLDLTRAEFNRLTADLVERSLGPCKRAMDDAKLKPSEIQEVLL
                     VGGSTRIPAVQEAVEKFFGKTPNRSLNPDEVVAIGASIQGAILSGDEKVSDVLLLDVT
                     PLSLGIETLGGVMTKLIERNTTIPTKKSEVFSTAADNQTAVTIHVLQGERPMAADNKS
                     LGRFDLVGIPAAPRGIPQIEVTFDIDANGILHVSAKDKGTGKEQSIRIDRAGSISKED
                     IDRMIKDAELHSEEDKKRQEFVAAKNELDSLIFSTEKSLSEYGDKLSSSEKTELEEEI
                     KNAKERMERASDAAELNEVKENLAKKAQHLGEVIYAHMQQQQSASGEGYNPNESGYNP
                     EDYTQQGPQEEESPKQEGPIDADFEVVDDK"
     gene            5444..6601
                     /gene="dnaJ"
                     /locus_tag="PLB65_05925"
     CDS             5444..6601
                     /gene="dnaJ"
                     /locus_tag="PLB65_05925"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOQ78102.1"
                     /translation="MEKRDYYEVLGVDRNADEATIKKAYRKLAMQYHPDKNPDNKEAE
                     EKFKEASEAYEVLSDKEKRQIYDQYGHSGLENKFGGAGFSWEEFMHRSDLNDIFGDGF
                     SSIFDTLFGGGFGSRNHRSSSGYNRGEDLQIELSLSLQEIAKGVEKTIKIGIKEPCDK
                     CGGTGSAEGKTETCPTCKGTGQIRQIRQSLFGRMQTISECPNCQGEGKVIKNKCSKCY
                     GEGRVGNVREIKVKIPPGVAEGQYVRLRGLGNVGARGGNRGDILVLIHEKQDELFERD
                     GYNIVLEYPISVSQAVLGDEIIVPTLTGSVKMKVPAGTQSGRLFRLKGQGIQSPNSSS
                     RGDQIVKIVVVTPTKLSRDEVELYTRLREFDKKRDLKPGKSFVSKLRSYFL"
     gene            6607..7308
                     /locus_tag="PLB65_05930"
     CDS             6607..7308
                     /locus_tag="PLB65_05930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HOQ78103.1"
                     /translation="MPSIYVPELSPDKRSITIAGEEYHHLVNVRRVKIGEIIKLNSGT
                     GVIAEGKISELTKRSVVIELLELVEYPYPEKNFAIAFALLKNKHDELLIEKCTELGVS
                     TFYPMITDFSVRIPSKNTVNRFRQIAVSAIKQCDNPWLPMINEPLPLTETIDKVLSER
                     WDPVLCSEQKPPKRISNLSSNIKPCFIIGPEGGYSEKEFAFFIKKDILQISICPLITR
                     AETAAIAAAAQFQLL"
     gene            complement(7303..7518)
                     /locus_tag="PLB65_05935"
     CDS             complement(7303..7518)
                     /locus_tag="PLB65_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78104.1"
                     /translation="MEQHTHLLLFIHRQASHSSFEAVITTLLLLVYSNLAIEIGGKPT
                     QQLGSQFGLSKSEFAKSLFFDCIIGVL"
     gene            7605..7841
                     /locus_tag="PLB65_05940"
     CDS             7605..7841
                     /locus_tag="PLB65_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HOQ78105.1"
                     /translation="MNNKKLKILNPIMFIAIFTTFVAMLTYKISGFFNYDAYGFKELH
                     EICGFIFVILAIFHIVYNWQWIKSQIFGIKPKKK"
     gene            complement(7975..10593)
                     /locus_tag="PLB65_05945"
     CDS             complement(7975..10593)
                     /locus_tag="PLB65_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOQ78106.1"
                     /translation="MRKIIVIMAILMWVGQVHALILRVSGDGTQDFEQIQSAIAQSCD
                     GDTVLVYPGRYYENLSMMGKNITLASLELTTGNLEYKYNTIIDGGRNESVISIKNGES
                     ELTIRGFTITNGSGHMDLTTRLFRGGGIFLIGQGNVRNFYLINNVIRNNTASTGGGLF
                     ISTSNVFLSGNSIYDNTADAGGGIRYQDNPLNLWSLTFDPVNRCNIYNNLAAQGSDIS
                     TYWESYTAVIVDTFTVANPWNYYASGIAYDHNIVNPFTFDILHTTGHQEINQDLYVAP
                     WGSDSNSGTSPQEPFQTIFKAMYMLASDSQNPKKVYLAEGVYSPSQNNQRFPCPVKTN
                     TSIVGVSTEQTIIDGDGYSGIFVGVPHSENLSISNVSGKNISGFFATSCSNTISLKNI
                     NISDMLYILSALGISMSLSQNIEMQNISINNIIGLDYVTRGIMITSCSGTLDMTDIHI
                     SHITAESEMIGLDVSFCGEANINRLRIDNCYSPSNQHYDMTTIMQIVANENGQSLKLN
                     LSNSIFADNYQAYSTDMMISIVAKNDSLSIQNCTFTGNSGGNYTIYIIGNAYLKNNIF
                     WNPAMPYEIKFKYFSEIGPTTLYLENNDIRGGYAGIVNTSPLNTIIWGPGNVNTDPLF
                     RMQTEQPYSLSENSPLIDAGILDPTAINVGLYDVAMNERFWDGDNDGIARIDIGAYEY
                     QQMLSPYNLNSEVNYNTVNLFWEMQGSNRALTGYRIHRNGSVIAEITDPTQLYYTDTI
                     TANGVYRYYITAAYGLLDSDPSNVINMLIEGLDIEENLQTSAQTALHLSPNPFYSTAD
                     IQYKVIRAGKVKVDVYNIKGQHIKSILEETKSSGSYQLIWDGKDKAGNKVAPGIYFMR
                     LEADGKALTKKMLKLK"
     gene            complement(10671..12905)
                     /locus_tag="PLB65_05950"
     CDS             complement(10671..12905)
                     /locus_tag="PLB65_05950"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78107.1"
                     /translation="MKNIAQLIIIVTILSVSSLLSAVDLYVGNPAVHPGTGTINDPFE
                     SIAYALTQLNPEEDYCLVFMPGLYNNQDQHYINRQLGLRSYTNNPEDVVIAETFYIAS
                     LSEPVYEVDFSGITFRGNNNNETIIHWSALTIDNCIFEDYIYGTPLNFDIDVSNVNIS
                     NCTFRNNHTAISDMNGNRTPGFIYNCLFYGNNIAIEINGANLENCTIADNELANQGRF
                     GANTYRNCILWGNGALNNGLPSVYYYCDTQSAVTGANNISVDPRFEDALNHIYTLKWD
                     VNGPSPCIDAGDPHLGYDPDGTPNDMGYRYYPHEIETYNFDRTTPERGIFWRCFPVVD
                     NISAPEGQPWNELGCMFSSYMDGDPTPLQKIYWNYVGSPHGIMSYNIIDQTWNNYDYQ
                     VTAPKGFILSFDPNNPIENLIVSGFRADPHTVPVELWVTDGGSYFSNWVGYFVPYTQN
                     VVTSLSNLIPNGQGHTYLDYIYSMKTQTWSTSRRERILGSPWIVDPSRFTFSEGTMVM
                     LELIDHAPSEMFWFCWGTPVDAYIRTAPTQFSYTEKIDYIPLYVELDPENMPEEIGVF
                     ADGECIGAVVVDNEVMDINLYPESAKGNDEIELSFYYGTKGKAVKQDYQIYDPNSYRF
                     VPGSLKLSEIKDYGYISLKGNSDSESVTSIVKLNQNYPNPFNPSTQISFYLAQDLPVK
                     LDIFNVKGQKVKTLCSSELKKGTYSYIWNGDDQLGKAVASGLYFYRLSTPEGVFSNKM
                     LLMK"
BASE COUNT         4518 a         2386 c         2684 g         4042 t
ORIGIN      
        1 aaagtattta gccggttcca aatagatata aaatatctaa ccgatattcc tgaatacgaa
       61 atctggagat gggaacatca tttccctaaa tatcttatct ctgccaggga ctataaaaca
      121 ggtgctatct atcttgctta tacccaagaa aaatcttcta tggctacagc cctgtttgtc
      181 gattatctac tttcttctct tcaatactat ggcatagatc cccaatctat tgttatccaa
      241 actgataatg ggaaagaatt cactaatcct actgaccgaa aagtaactct atttgaaaaa
      301 ttagttaaag aaaaatatca agcaactcat tttcttattc cctatcgcag acctacttgg
      361 aatagtgatg tagaatcctt tcataacctt attgaagaag aattctttct ttccgaaaaa
      421 ccaaatagtg aaattgatct cttggctaaa accttcgctt accaaatctg gttcaattcc
      481 tttagaaata atagaggtag aaataatcaa aatcctgaac aaatcttcca gtcagaattg
      541 aatactatga aacctcttcc taatatccct cctattttgg ttgacaaata tatacagctg
      601 attgcaaagg ataatgaagt tggatacttt ttacctttac cacccagtta ggatggaaaa
      661 taaaaaacga atggatgagg agaaagcgct acatgataaa gattgatatg attaccagga
      721 acaggaagca attccttgac ctcttgttat tggctgacga gcaagaggat atgattgata
      781 gatatctgga aaagggcgac atgtttgccc tgtatgatga tgatttgaag agcatttgcg
      841 ttgtagtacc gatagacagc gatacctgtg aattgaagaa catagcgaca tacgaaaaat
      901 accagggcag aggctacggc agaaccctgg taagctatat ttgcgattat tacaagaaag
      961 actacaaaac gatgctggtg gggactgggg agactccggc aagcttgtcc ttttatgaag
     1021 gctgcggatt ccaaagatcg catcgtctga agaattttat gatagacaat tatgaccatc
     1081 ccatatttgt aggcgatatc caacttattg atatgatata tcttaaaaag gatcttggtg
     1141 gcggcgatcg ctgaatcgcc gtcaatatat aggcggaacg gcgtccacct gtacggtttt
     1201 tctatccaca gattaacaca gattatcacg aattatcaat tgatttctct tggatttcac
     1261 gcagatttac gcagataaac aacgcagatc aggcagatga cttggggtga aggggacgta
     1321 tcgaaccctc accgctgcgg aacgttcaat agcgaaggcg aaacttaagc atgcaacaag
     1381 ccgaagatcc aaaccgctat tattcatcat tcatttcttc attcttcatt tgaatgtgga
     1441 tagtgaagaa aagagataac gggaaatatt tttattagca ctctcacttt tcgattgaca
     1501 attttccaat cttgattaaa ttgtctttag attagcagtt catactatgg actgctaaga
     1561 aaatataaga ggcagagata atgaagaaga atcagcttag attaaacgaa acactaaagt
     1621 cgctggtgga tgaatatatt ctcagttgcg aaccggtgtc atccagaata ctaaacgaga
     1681 aatatcttca gaaagtatcc tcggccactt tacgtctgga tttattgaag ttagagaaaa
     1741 tgaacttgat atcccagccg catacttctt caggaagagt tcctacaatt aagggctatc
     1801 gagaatattt aaaactgatt gagccggaca tagaaaagac cagatatgaa gggatggatt
     1861 tgttaaggga gctattggtt cgtaactata aagatacacc cagggcttta cattatataa
     1921 tggagaattt agctaaggaa acagaccagc tttcttttgt tgccgaacct gaggtttcga
     1981 atggttattt ggctaagttg gaggtatttt ccattgggga aaacaagcta atatttgtta
     2041 tgagtttaga ttccggttta gataaaacgg ttataatgaa atgtgataat gaattaactg
     2101 aatcacaatt gaagaaactt gtacgttatt tgaatgacga acttgttgga ttacagatat
     2161 atgacattgc caatcgagtc ctagtggaaa tgcaggaaaa ttctgataat ggtgtaatta
     2221 gctggttttt ggttgaattg cataaagctt ttatggaaat aagtgatttc tttattcatt
     2281 atgacggcag catcaatttt ttagagcaga aggaatttga tactaaagag aatatcttga
     2341 agtttatgag tttattacaa aaccaggatt tcctacttaa tatgatgcgt aaacatcaga
     2401 gtaataaaga aaccaatgta gttttgggag atgatttcac aaaaagcaat tggttagatt
     2461 ttgctttaat ttactctaaa tatgagatgt ttggaatacc tggttattta ggagttttgt
     2521 cacctattag aactgaatac cgtaaactaa ttccccttat tagggatgtg gctaaaacaa
     2581 taacaaatac aacccggcgc ggaatgatcg tgcctcagga gaaaatataa tgaataaaaa
     2641 gacgcaaaaa gaaccggaaa ataacaaaga tatacaggta gaagaagtta ttgaagaaca
     2701 gaaaacggat aaaattcaag aactggaaca ggaaatagcc gaatggaaag ataaatacct
     2761 acgctgtatg gctgaatttg agaatttccg aaagcgttct atagctgaaa aagcagattg
     2821 gatcagatta gcaactcaga aatttgcttt ggagatctgc gatgttttag ataatttcga
     2881 aagagctatc ttacagggta gtgaggaaga aaaatctact ccttttggga aaggagttct
     2941 gatgattgaa cagcagcttc gtaaagctct tgaaagagaa ggagtaagaa aaattgaagc
     3001 actgggacaa cccttcaatc cagagttcca tgatgctctg gctcatattc ccagtgattt
     3061 agaaaacaat actgttgcag ccataattca aaatggatac acaatgcacg ataaggtgtt
     3121 gcgtccagtt cgagtagctg tttccaacgg ttcaaaaata aataatgagt ctcaagaaga
     3181 ataaaatctg tgctggagct tttcagagct ctacagcaaa caaaaaataa ataatgaatc
     3241 ccaggaggaa aaatgggaaa aataatagga attgatctcg gaacaacaaa ctcctgcgtt
     3301 gccgttgttg aaggcggcaa accagtagtt attaccaatg cggaaggaag cagaacaact
     3361 ccttctgtag ttggttttac aaaggatggt caacgtctgg tagggcaatt agcaaaacgc
     3421 caggcaataa ccaatcccca gaatactgtc ttctccataa aaagatttat gggaagaggt
     3481 tacgatgaag taacagaaga aattaagcag gtccctttta aagtcatccg tggcttaaag
     3541 aatcaagcag cagtgcatat tgatatggaa aataaggatt atactcctca agaaatttct
     3601 gctatgatcc ttaccaaaat gaaagaaaca gcagaagcat atctggggca aaaagttaca
     3661 gaagctgtta tcactgttcc tgcttatttt aatgatgctc agcgccaagc cacaaaagat
     3721 gccggacgca ttgctgggtt agatgtaaag agaataatta atgaaccgac tgctgcagct
     3781 ctggcttatg ggatggagaa caaaaaggaa caaaaagtag ccgtatatga cttgggtggg
     3841 ggaactttcg atatcagcat tctggatatt tctgagggtg ttgtggaagt gctttctacc
     3901 aatggcgata ctcacttagg tggagatgat tttgataaac gggtcattga ttggatattg
     3961 gaacagttca aaaaacagga agggatggat ttatcccgcg atccgatggc tatgcaaaga
     4021 ttacgggaag cagccgaaaa agctaaaatt gagctttctg gaacactgac caccaatatc
     4081 aatctgcctt ttattactgc tgatgctaca ggtcccaagc atcttaatct ggatttgacc
     4141 agagcagagt ttaatcgact aactgctgat ttggtagaga gatctttagg accatgtaaa
     4201 agagctatgg atgatgcaaa gttgaaacca tcagagattc aggaagttct tttagtgggt
     4261 gggagtactc gtattcctgc tgttcaagaa gcggtagaga aattctttgg taaaacacct
     4321 aaccgaagct tgaatccgga tgaagttgta gctatcggag cttcgattca gggagccatt
     4381 ctctctggtg atgaaaaagt tagcgatgta ttactattag atgtaactcc cctatcttta
     4441 ggtattgaaa ctttgggtgg agtaatgaca aaattgattg aaaggaatac tacgattcca
     4501 actaaaaaaa gcgaagtttt ctccacagca gcagataacc aaactgcagt gactattcat
     4561 gttctacagg gtgagcgtcc aatggctgcc gataataaat ccttagggcg ttttgacctg
     4621 gttggaattc cagctgctcc aagaggaatt ccccaaattg aagttacctt cgatatcgat
     4681 gccaatggca ttttgcatgt ttccgcaaaa gataagggaa caggtaaaga gcagtccatc
     4741 agaattgatc gtgccggaag tatcagtaaa gaagatatcg acagaatgat taaagatgcc
     4801 gaacttcatt cggaagaaga taaaaaacgc caggaatttg tagcggcgaa aaatgagctg
     4861 gattccttga tcttcagtac ggaaaagagt ttgagcgaat atggagataa attgtcaagc
     4921 agcgaaaaaa ctgaactgga agaagaaatt aaaaatgcta aagaaagaat ggaaagagca
     4981 tctgacgctg ctgaattaaa tgaagtgaaa gaaaaccttg ccaaaaaagc acaacattta
     5041 ggtgaggtta tctatgctca tatgcaacaa caacaaagtg ccagcggcga aggatataac
     5101 cctaatgaaa gtggttataa tccagaggat tatactcaac aaggacctca ggaagaagaa
     5161 tctcccaaac aagagggtcc tatagatgct gattttgagg ttgttgacga taaataattc
     5221 ataaacagga tcagacgaat tctaacggat tgacacggaa taaaactaac aaatagaatt
     5281 ccgtgttaat ccgtctgatc cgaatgataa ataaaagttg aggtaattaa tagcgaaaaa
     5341 aagaaggata gttttgtaat tgagtgagaa acttaatatg gaatcatctg agaagcatta
     5401 agtttcagct atgaaatcaa tgaaatctgt gagaggttaa ttaatggaaa aaagagatta
     5461 ttatgaggta ttgggcgttg atagaaatgc cgacgaagct actataaaaa aggcatatcg
     5521 aaagctggca atgcaatacc atccagacaa aaacccagat aataaagaag ctgaagagaa
     5581 gttcaaagaa gcaagtgaag cttatgaggt gttgtcggat aaagaaaaaa gacaaatata
     5641 cgatcaatac ggtcattctg gactggaaaa taaatttgga ggagcaggtt tctcctggga
     5701 agaatttatg catcgcagtg atcttaatga tatcttcggc gatggtttta gcagtatctt
     5761 tgatactctt tttggaggag gttttggaag cagaaatcac cgcagttcaa gtggttacaa
     5821 tagaggagaa gaccttcaaa ttgagctttc attatcactt caggaaatag ctaagggtgt
     5881 tgaaaagacc ataaaaattg gcattaaaga gccttgtgac aaatgtggtg gaacaggaag
     5941 tgcagaagga aaaaccgaaa cctgccctac ttgtaaggga acagggcaga ttcgccaaat
     6001 aagacagtca ctttttggtc gcatgcaaac tatttccgaa tgcccaaatt gccagggcga
     6061 agggaaagtt ataaaaaata aatgtagtaa atgctacgga gaaggacgag taggtaatgt
     6121 ccgcgaaata aaagttaaaa ttccccctgg agtggctgaa gggcaatatg ttcgtttaag
     6181 gggactgggt aatgttggag cgcgaggtgg gaatagagga gatattctgg ttttaattca
     6241 tgaaaagcag gatgaactct ttgaacggga tggatataat attgtattag aataccctat
     6301 tagcgtatct caagcagtgt tgggagatga aataattgta cctactttaa ctggttcagt
     6361 aaaaatgaaa gtccctgccg gaacccaaag tgggagatta tttcgtttaa aaggacaggg
     6421 aattcagagc ccaaattctt cttcgcgcgg cgatcaaata gtaaaaatag ttgtagtaac
     6481 tccaactaaa ctctctcggg acgaagttga attatataca cgcttacgcg aatttgacaa
     6541 aaaaagagac ctaaaaccag ggaagagttt tgtttcgaaa ttgaggagtt acttcttata
     6601 aatattatgc cgtctattta tgttccggaa ttaagccctg ataaacgatc tattaccatt
     6661 gcaggtgagg aatatcacca tttggttaat gttagaaggg taaaaattgg agagataatc
     6721 aagctgaata gtggcactgg agttattgca gaagggaaaa tatctgagct taccaaaagg
     6781 tcggtagtaa tagaattact cgagttagtc gaatatcctt atcccgaaaa gaactttgcc
     6841 attgcatttg ctcttcttaa aaataaacac gatgaactcc tgatagaaaa atgtactgaa
     6901 ttgggtgtta gtactttcta tccaatgatt actgatttta gtgtgcggat tccatcaaaa
     6961 aatacggtta accgatttcg acaaattgct gtatcagcta taaaacagtg tgacaatcct
     7021 tggttaccaa tgattaacga acccttacca ctaacggaaa caatagataa ggttctctct
     7081 gagagatggg atcctgtttt atgctcggaa caaaaacccc caaaaagaat atccaattta
     7141 tccagtaata ttaaaccctg tttcattatc ggtccagaag gcggttacag tgaaaaggaa
     7201 tttgcttttt ttataaaaaa agacattctc caaatatcaa tttgccctct tataactaga
     7261 gcagaaacag cagctatagc agctgcagct caatttcagc tgttataaga ccccaataat
     7321 acagtcaaag aaaagtgatt tggcaaattc cgatttgctt aatccaaatt gggaacccaa
     7381 ttgctgtgta ggtttacctc caatttctat tgccaaattg gaataaacta aaagtaatag
     7441 agtagtgata acggcttcaa aagaagaatg gctggcttgt ctatgtatga ataacagcaa
     7501 atgcgtatgt tgttccatcc tctcgttatg ttctaattct acaatgtaag gttaatttgc
     7561 ctaaaaggga atttcgatta ttatatcaaa aaaaggagta tgaaatgaat aataaaaagc
     7621 taaaaatatt gaatccgatt atgtttatcg cgatctttac tacttttgta gcaatgctta
     7681 cctacaaaat ttcaggtttt tttaattatg atgcctatgg cttcaaagaa ctgcatgaaa
     7741 tatgcggctt tatcttcgtc attttggcta tttttcatat tgtctataac tggcaatgga
     7801 ttaaaagtca gatctttggc ataaagccca agaaaaaata gagtggccgt aagccccatt
     7861 tctttacgag aaataaaagc ggagcttcag cgagctccga ctacaattta atttactatc
     7921 attttatgag gtagaaaagg acaccaacct tccggtaaga agaacaactt tctcttattt
     7981 tagctttagc attttctttg tcagagcttt accatctgcc tccaggcgca taaaatagat
     8041 gccaggggca actttgttac cggctttatc ttttccatcc caaattaatt gataactacc
     8101 agaggacttg gtttcctcca gtatactctt aatgtgctgt cctttaatat tatagacatc
     8161 aaccttaact ttacctgctc taatcacctt atactgaata tcagcagtag aataaaaggg
     8221 attcggactt aagtgcaaag cggtttgtgc agaagtttgc aggttctcct ctatatccaa
     8281 accttctatt aacatattaa taacattgct gggatcggaa tctaacaaac cgtaagctgc
     8341 agttatataa taacggtaaa ccccattggc agtaattgta tcggtataat acaattgagt
     8401 tggatcagtt atttctgcta tcacagaacc atttctgtga atccgatagc cagttagggc
     8461 tctatttgaa ccttgcattt cccagaagag atttaccgtg ttataattta cttcggaatt
     8521 aagattataa gggcttaaca tttgttgata ttcataagct ccaatgtcaa ttctggctat
     8581 tccatcatta tctccatccc aaaatctttc attcatagca acatcatata gccctacatt
     8641 tattgctgtg ggatcgagta ttccagcatc aatcaaaggt gaattttcac ttaaggaata
     8701 gggttgttca gtttgcatcc gaaacagggg atcggtattg acatttcctg gaccccagat
     8761 aatagtattt aaagggctcg tatttacaat accggcatat ccacctcgga tatcgttatt
     8821 ctctaaatat agtgtagttg gtcctatttc tgaaaagtac ttaaatttta tttcataagg
     8881 cattgccggg ttccagaata tattgttttt cagataagca ttccctataa tatatatagt
     8941 ataattgcct ccactatttc cggtaaaagt acagttctgg atggataaac tatcattctt
     9001 ggctactatg gaaatcatca tatctgtact atatgcttga taattgtcgg cgaatatgga
     9061 attacttagg tttaatttta agctttgccc attttcattc gctactattt gcattattgt
     9121 agtcatatca tagtgttgat tactgggaga atagcaatta tctattctta aacggttgat
     9181 atttgcctcg ccgcaaaagc taacatcaag cccaatcatt tctgattcag cagtaatatg
     9241 actgatatga atatcagtca tatctaatgt tccactacat gaggtaatca ttatccctct
     9301 ggtaacataa tctaatccta ttatattatt tatagatata ttctgcatct ctatgttttg
     9361 ggacagactc attgatattc ctaatgctga caaaatatag agcatatcag atatatttat
     9421 gttctttaaa gatatcgtat tagaacagct agtggcaaaa aatccggata tattttttcc
     9481 tgagacattt gatattgata gattctcact gtgaggaaca ccaacaaaaa tacctgaata
     9541 accgtcacca tctattatgg tttgctcagt tgaaacacca actatacttg tattggtttt
     9601 tacaggacag gggaaccttt ggttattttg gcttggggaa taaacacctt cggcaagata
     9661 gaccttttta ggattttggc tatcggaagc aagcatatac attgccttga agatggtctg
     9721 aaaaggttcc tgagggcttg ttccgctatt ggaatcactt ccccaggggg caacatagag
     9781 gtcttggttt atttcttgat ggcctgtagt atgcaatata tcaaaagtga agggatttac
     9841 tatattatga tcataagcaa ttccactggc ataataattc cagggattgg ctacggtaaa
     9901 tgtatccact attacagccg tataactttc ccaataagtt gaaatatcag aaccctgggc
     9961 tgctaagtta ttgtatatat tacagcggtt taccggatca aaggtaagag accataaatt
    10021 taatggatta tcctgatatc ttatacctcc acctgcatct gcagtattat cgtagataga
    10081 attaccacta aggaatacat tggaagtaga aataaataga cctcctcctg tagaagcagt
    10141 attgtttctt attacattat taataagata gaaattgcgt acattacctt gtcctatcaa
    10201 aaaaatacca cctcctctaa acaatcttgt tgtcaaatcc atatgaccgg agccattggt
    10261 tatagtgaac cctcttattg taagttccga ttcgccattc ttaatgctaa tcacactttc
    10321 gtttctgcct ccgtctatta tggtgttgta tttatattcc agatttccgg tggtaagttc
    10381 caaactggca agggtaatat tttttcccat catactgaga ttttcataat acctgcccgg
    10441 gtaaactaag actgtatcac catcacaact ttgagcaatc gctgactgta tttgttcaaa
    10501 atcctgagtt ccatctccgg aaactcttaa aatcaatgca tggacctgtc ctacccacat
    10561 taatattgcc attattacta taatcttacg cacttccagc tcctttacct tttctaaatc
    10621 cggagcccgc taaataaaag cgagttccgg tttggttcaa tattctttgt ttatttcatt
    10681 aacagcatct tattgctgaa tacaccttcg ggagttgaaa ggcggtagaa atataacccc
    10741 gaagcgacag ctttccctaa ttgatcatca ccgttccata tatagctgta ggttcctttt
    10801 ttaagttcag aggaacagag ggttttcact ttctgtcctt taacattgaa aatatccaat
    10861 tttacaggca agtcctgtgc aagataaaag gatatttgag tactcggatt gaagggattg
    10921 ggatagtttt gatttaattt tactatactg gttacagatt cactatcgga attacccttt
    10981 aaggagatat aaccataatc cttaatttcg cttaatttaa gtgaaccggg cacaaagcga
    11041 tagctattag gatcataaat ctggtaatcc tgttttacgg ctttaccttt ggttccataa
    11101 taaaaagaga gctcaatttc atcattgcct ttggcagatt cagggtaaag gttaatatcc
    11161 ataacttcat tatcaactac tactgcacca atacactcac catcagcaaa aacaccaatt
    11221 tcctcgggca tattttccgg atcaagttcc acatacaaag gaatataatc tattttttcc
    11281 gtataactga attgagtggg agcagttcta atataggcat ctacaggtgt tccccaacag
    11341 aaccagaaca tttctgaggg ggcgtgatca attaattcca gcataaccat tgttccttcc
    11401 gagaaggtaa aacgggaagg atccacaatc caggggctac ctaagattct ttcccggcgg
    11461 gaagtgctcc aggtttgggt tttcatactg taaatataat ccagataagt atgaccctgt
    11521 ccattgggaa taagattgga taaagatgtt acaacatttt gggtgtatgg aacaaaataa
    11581 cccacccagt tagagaaata tgagccacca tccgttaccc ataattcaac cggaaccgta
    11641 tgtggatcag ctcggaatcc gctaacaatt aaattctcta tcggattatt aggatcaaaa
    11701 ctaagaataa aacctttggg agctgttacc tgataatcat agttattcca ggtttggtca
    11761 attatattat aggacattat gccatgtggg gaaccaacat agttccaata tattttttgc
    11821 aatggtgtgg gatcaccatc catatagcta ctaaacatac acccgagttc attccagggc
    11881 tgtccctcag gggcggagat attatctaca acgggaaaac atctccagaa aattccccgt
    11941 tcgggagtag ttcgatcaaa attgtaggtc tctatttcat gcggatagta gcgatagccc
    12001 atatcattag gagttccatc aggatcatat cctaaatgag gatcaccggc atcaatacaa
    12061 ggactgggac cattcacatc ccatttaaga gtgtaaatat gattaagagc gtcttcaaaa
    12121 cgggggtcaa cagagatatt gttggctcca gttactgctg actgcgtgtc acaatagtaa
    12181 tagacagaag gtaagccatt attgagtgct ccatttcccc acaggataca gtttctatag
    12241 gtgtttgctc caaaacgacc ttgatttgct aattcattat cagctatagt acagttttcc
    12301 agattagcgc cattaatttc tatggcgata ttattaccat agaatagaca gttatagata
    12361 aatccgggtg tcctatttcc gttcatatca cttatagcag tgtgattatt tcggaaagtg
    12421 caattggaaa tgtttacatt acttacatca atgtcaaaat ttaaaggagt accataaata
    12481 taatcctcaa agatgcaatt atcaatagtt agagcactcc aatggattat tgtctcatta
    12541 ttattattac cccggaaagt tattccgcta aaatcaactt catataccgg ttcacttaaa
    12601 gaggcgatat aaaatgtctc tgcaattaca acatcctcag gattattagt atagcttctt
    12661 agtcctaatt gccggttaat ataatgttga tcttgattat tatataaccc gggcataaaa
    12721 accaagcagt aatcttcctc agggtttaat tgggttagag cataagcaat tgattcaaaa
    12781 ggatcattta tggttcctgt tccgggatgg acggcaggat tacctacata tagatcaaca
    12841 gcactaagta aactgctaac tgaaagaatg gtaactataa taattaactg ggcaatgttt
    12901 ttcatagaaa acgccattct gttgttactt aaatgggggg gggtagaatt aaagattttt
    12961 tcattttttt tactcctaat tttaggttag aatgaaaatg ataggtttaa gtttaaacat
    13021 aaagatcgat gttcattctc gaacttatga gttcacttat accaagccag tttacgaaat
    13081 tcttctattt aatattgcag ataagctctg cacccaaaga tacagctatt atcatattcc
    13141 ctccttttta aggaacattt tttagctctt atattttagc ttttggggca acacactaat
    13201 atgtaaattt tcaatccata tagaaatgtt gcataattaa atttcctctc accaaaaatc
    13261 acactattag cagattctgg aatcacattg aacaaaaatc attatatata ttttcagtat
    13321 tttctgtcaa tatatttatt cttgttgaac gagtttttat ctatcaattt taggaattcg
    13381 atagaattaa aatagtttat aataaacaag gattttggaa acaaggattt aaggattgga
    13441 ttttagaaac gaggatttaa ggattttagg atttagagga tttcttcttt aaagttccgt
    13501 taggaacgat acatactaac gacgggtttt aacccggcga caagaagaac gaataaaaaa
    13561 agtcccagcg ggacgacaga tttagtttta gaaacaagga ttttaggatt ggattttcga
    13621 aacgaggatt
//
LOCUS       DAOSTH010000044        13602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_4609_length_13602_cov_12.602569, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000044 DAOSTH010000000
VERSION     DAOSTH010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13602
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4609_length_13602_cov_12.602569"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..1068
                     /locus_tag="PLB65_05955"
     CDS             97..1068
                     /locus_tag="PLB65_05955"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOQ78108.1"
                     /translation="MKIAITGANGFVGSNLVKHFLSAGYEVRAIVRSNAFTTFISPRA
                     QIVKVNYYDAQSLISAFTGIDIIIHNAGKTKALNSGQMFKANLGITEKIIEAINKLPE
                     EQQLIYFSSQAASRPSIDNIPVKEDEPSAPVTWYGKSKFAAEKAIKEKCRKNYTIIRP
                     CSIYGCGDKDFLQVFKLVNRGFGFKIGRKDKLINMIYVLELCRFVELCVNNPAAYSQT
                     FFATDGKVYKQSDVIKAVAKALNKKPVTITVPEFLACLIFYAGDLYGRLFHKEVVTNR
                     EKMKEIMADAWLADTEKAIRILGWKPTTNLENNLKETAKCYHDLGWL"
     gene            1041..2726
                     /locus_tag="PLB65_05960"
     CDS             1041..2726
                     /locus_tag="PLB65_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78109.1"
                     /translation="MLSRFRLVVVFTLFTIPFFAQKITTAEPTKVDTLELVEKKVTKA
                     EIENWIKANHLDTNEHIFGASPLHIDYGDIYHNGYFYVGRSEMNMPERNGFETLSGIF
                     SPALYYGYFNSLYQVKEYQRTVNYTNNSYPFPPVLTSLKAGLGDYDHHYARINLSKNE
                     LFSYPYLEYQGDFLVQNGYWTDITSTETSMKHRLSQSGKLVTWELEFANWKKDIAMNE
                     LLPVYWQSTNFTINHNLQQFYASFALPWFKLKLLQESESANTNLFYKEYNRNSTRLQF
                     SAQQKIGFLQTEELYEHNWNKGISCFCGFYNAEEYQDKLGFGFQINNPVKINLTADCL
                     DWKRYRLFANADYSLKNFNLGATHEHKMGDDSSVLKVKDIFNADGELDLIDISVSQES
                     SVYCSYNLFNIKPLVAVGTREIEQEAENSWLNIKKQQPFLRLAMDVNYSWKNWLITAR
                     PEWVISKYYPSLTENPEYRFHSVQQIKYILPYDNAVVVGCDIYGHSEYYVANALNPYL
                     IESSTMLDAWAGINVGNLFELRTGFKNVLSSSLYGTYPVPVSVYIDVLWLYLN"
     gene            2989..3792
                     /locus_tag="PLB65_05965"
     CDS             2989..3792
                     /locus_tag="PLB65_05965"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOQ78110.1"
                     /translation="MKLFETHCHLDHPDFDSDRESLINKCFSSGIEYIINVAFNKETA
                     FSSLGLAKKHLHIFSTVGYHPHDAIDFDAEIVKKLAREKKVVAIGEIGLDFFRNLSPF
                     TVQREVFANQAHLAVEYDLPVIVHDRDAHQECYNILKNENIKEAVFHCFSGDIIFAQQ
                     VLDAGWMISFTGNITYANIHLDDVIRLVPLDRFMVETDCPYLPPHPHRGERNSPLYLP
                     LVVEKIAEVKGISPKEIAELSFANAYRFFRIPPDPVKALHHKSSHKKVN"
     gene            3840..5045
                     /locus_tag="PLB65_05970"
     CDS             3840..5045
                     /locus_tag="PLB65_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78111.1"
                     /translation="MKKAVIIICGAILCISLQAGNSIFSFYGFPVHNYGRDIYSLGMG
                     DTGASDVFRINTGYANPAMHNRNNYTLFSTGMNMGYTKYHSQYEGIERTFRDDSLDFP
                     YFNINIPIGRNRLGVQFNSLASGLVTNYHYLPDGSVESQTSDKYLYKADLIYSYSFSE
                     VNLGISANYYFGHNKQYFSQTGNYSNFDTRESIIMDFKNPSITVGTIKLFDKYSVGAH
                     YCAPVTLKGELIRSSIHNTEPAEDYKFKLPEQYCLSATALPFEQFKVAADINYEPWST
                     ISDDYRDCYKIGLGLAYEPNMEAHKNIFMRFPMRVGASMRQLYFRDKDGKDIDEVSLS
                     CGLTLPLKNEIDRIDLGFQYLKRGDLATNNLSENSYMLMIGFTGFDIISKAPDRTAPR
                     NIPVKEEVE"
     gene            5042..6598
                     /locus_tag="PLB65_05975"
     CDS             5042..6598
                     /locus_tag="PLB65_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HOQ78112.1"
                     /translation="MKPDEPNSNMDSSNDILNQILKSRNLSQQDLQTNLNMLPDEALF
                     ANIDKVEHRIRAALYENEPMVIFGHDDPDGITSTYILYNFLNSCGYQKHRYYIPNRNL
                     EPHGIQAGFIDFVREGNYKLIVTVDNGIASLEGVKQLNALGCDVIITDHHIVQPEVLP
                     PAYAIMNPQLPQCDYPFKQLAGVGVVLMLIRYLGNKWEHPIEPASYFWTAVGSVADKV
                     PMIGMNRILVRYALEHFYEIQDDTVEFLLRNYNRINTLSDINSFLQYTAHLLANGRDD
                     KGRHTALRFILQLSDAKADLFQELENQKNKWDSDLNRVFNFLDTLAADFVGNWFVYYD
                     DEDIIPYPLLGTAATYILNKLNIPTLLLKSHNGDTVCEGRCSEGFNIMDAFTHCKKYL
                     KQFGGHPKAAGFTMQQENYDTFLDCFNNFLLKHFHPSHTASTKYDAVVAAKDINRENW
                     QKLEILLPWGQLNPEPTLLIRNTSKAEIMNYVALDNSGREIPNQGKGDILALWKAANM
                     IKVLSWQQTD"
     gene            6627..7214
                     /locus_tag="PLB65_05980"
     CDS             6627..7214
                     /locus_tag="PLB65_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEC59/DGK1/VTE5 family protein"
                     /protein_id="HOQ78113.1"
                     /translation="MIQLGETLRKSIHLSSLIIPLGYRYLLSFNRSLGFSILLIAFTI
                     SLVIEFYRFWQKGFRKTFHRIFGIILRKHEFKDFTGATYMLFAAVLCVAFFPPLIASC
                     AMAFLTIGDTFAALVGINFGKRWYIKQNKSLEGSLACFVSCSVFGIFWLGSPFLAVLG
                     ALAATLAELSNIPIDDNITIPIFSALVMTLLSIVI"
     gene            7226..8209
                     /locus_tag="PLB65_05985"
     CDS             7226..8209
                     /locus_tag="PLB65_05985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOQ78114.1"
                     /translation="MKLSFVIPVLNEQDSLVQLYSEIIQNCNGYDYEIIFIDDGSTDN
                     SFNIMSQIALSDMKVKVVQFRCNFGKAAALQTGFNLTSGDIVFTLDGDLQDNPIEIPN
                     FIAKLDEGYDLVSGWKRKRRDPIYKTLPSKLFNFVTAKTFHLKLRDYNCGFKAYRHPL
                     EKEIILYGEMHRYIPVLAHSLGYKVGEIPVAHRPRKFGKSKFGKERYLRGFFDLLTVK
                     MITQYSKSPLYLFGRIGIASTFLGLIISIYLAILKIFMNQPLSNRPLLFLGILLILGG
                     LQFISMGLISELIVNRFRQGNRTPVSIAQYVNIDQIESKPYQQTNLPEVNE"
     gene            8202..9425
                     /locus_tag="PLB65_05990"
     CDS             8202..9425
                     /locus_tag="PLB65_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ78115.1"
                     /translation="MSKLELFFLSRYLKAPKRNLLRFSFVFMILGIILSVGILSAGLN
                     LFEGYERTLKDVLLGAFPHLTISKDNLGFISHSETKNLLNKMANCKEIQQVTPVLSYP
                     VMAAGKAKVRGATLNAYDFSNKKSFPQAKYIQKGKKIPASQEVIIGKYLAKELGKDIG
                     DTLKLVFTRLDNISALGIFPSEYYITVVGIYSSGFYETDRSLILANITDAENMLNSAS
                     GFSKIEIRLDPKYIDKAPEIGQKLQEIVGNYYSVYPWQTFSAGLLHLVTMEKWLIFII
                     FCFLVLIAGINVISAVSTIILDKKNEIAVLKTLGANSTSIKRVLSFQVGLSALLAILA
                     GQILGALLSWGIEKQNFYKLKGDVYFIDSLNTSIAPLNQLIIFIVSAALVFICIYYPL
                     KQIDKLEIIELLRNP"
     gene            9478..10149
                     /locus_tag="PLB65_05995"
     CDS             9478..10149
                     /locus_tag="PLB65_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ78116.1"
                     /translation="MVCLAGYDLCKSYLDSNQTINVLRKATIEVKEAELVCITGQSGS
                     GKSTLLHLLGLLDSPDSGKVMISGREINSTMPEAASIRNLELGFVFQFHYLIEDLNAQ
                     ENVALPMIISGVNSSQAIKKAAELLQALDLEDRINHYPNQLSGGEQQRVALARALINN
                     PKIVLADEPTGNLDPEHSAEVWDMIIKLNKERGQTFVIVTHDIVNASKAKVIYNLSEG
                     KLEKK"
     gene            10162..>13602
                     /locus_tag="PLB65_06000"
     CDS             10162..>13602
                     /locus_tag="PLB65_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78117.1"
                     /translation="MPRSKKFLIFVLILLLINTGFFLAWYAFGLNNFFKNSVAKYIGQ
                     LTKGKVTIEELHFSDRQLLAKGVNYTSADSSLAISINRLSARYNLTRYLLSGFKTNKL
                     VNDIEIYKPVVHYSYQYKPQPPKAKKPLVLPNFAKYFKHLQLNNGAAVLSFNIPVKIT
                     QEGNLTISESLSNINLEADNKSTTNATINALTSNGGKISAKGLLDKGRIVSVDGEISA
                     YKPLFVSHPDLQNLHTEISLVGSLKQDSLAAPLKYNAEAQIWGTETLFANNYPVLIPY
                     LWVKTDGNNLKLSLSKSNVGSSDLAAEVAISNLRHKIKFERVDISADVDLAMIQKDLK
                     GMVNANIIGEGTIDKPELELSLVSEQAAYQNYTFNKFNFSGTYQNDLLDFIIPELIFG
                     NQIINLTGSFNPLNMILDANLETKPINKKAQPYLATAKLDLYAELWGKYPYVDATIHQ
                     LDFAYELANIENVSGKIKLLPLPDVNDLYLDANLSSSNGFNIEVIGDVLDRNLLVDAK
                     FDDLSIAELYAQPTLVNLNPGLKGELKAIITGDNIVVQTLTGLTLDNPVPVDLTLDAL
                     GTINYKMKDASIYVSGTEGIVNNQPLNFELAAMLKDNIVTLQGLKVNDLINVSGAINL
                     ANYADMNIGLNINNLNYLDVVRYYPKLDVNLPEFNGLNVFLEYNRDNLQRLRANVFLS
                     DIDLLAVTPFSLELGMEGPLNNILVFSTIKNKEQNIVDMTGYVSLTPELNVNLDAAFK
                     DLHIQDVLINSPIYGIIDGISGITWHKVKEQKPELELRADLISRDIVLGNYTIDKAII
                     KGKQTPRKMIVDSLFVKSTGLFELSGSGAINYNALYNEFFEGPEHLNLRAQGELFTWL
                     NGLTPYIQEAKGSSSLSASIGTTEEQFMVSTGKINISGGYIQIKDQTEPCSNINIRGG
                     FDKNRVVIDYGQIQMGEGKLVFNNIFEVDNSDHIMLGFLDLGILRLMVEEPGIMINVP
                     KYTTPRSLTKVILKGNNSRYATIKGPFDQMKISGEVIASNTNVLYPPDTENLLTLATS
                     MRGTNKKSSSEPSPLPFTLDVKLIAGENVKYVTYPAKLRVVPGGYLHLMYDGLSFIAE
                     EASFSSEQGTVDIFGTIFQVDKVNLTLVDSQNLLGLEGIFFKRAPDGSMITLTAVTSN
                     D"
BASE COUNT         4363 a         2268 c         2534 g         4437 t
ORIGIN      
        1 atgctttaca cgcatgtggt cgggggttcg agtcccttag cgcccaccat tttttctatc
       61 ccaataaaaa ctgaagagtg taacaagcgg tgatatatga aaatagccat aacaggagcc
      121 aatggatttg taggcagcaa tctggttaag catttccttt ctgccggtta tgaagttaga
      181 gctatagttc gatctaatgc tttcacaact tttatttcac ctcgggcaca aatcgtaaaa
      241 gttaactatt atgatgctca atcccttatc tctgctttta caggtataga tataataatt
      301 cataatgcgg gaaaaaccaa agctctaaat tcagggcaga tgttcaaagc caatttaggc
      361 atcacagaga aaataattga ggcaattaac aaacttcccg aagagcaaca gcttatctat
      421 tttagttctc aggcagcctc tcgtccgagt atagataata ttcctgtaaa agaagatgaa
      481 ccttctgctc ctgtaacctg gtatggaaaa agtaaatttg ctgcggaaaa agccatcaag
      541 gaaaaatgcc ggaagaatta tactattatc cgtccctgtt caatttatgg ttgtggtgat
      601 aaggattttt tacaggtttt taagcttgtt aatcgtggtt tcggttttaa aattgggcgt
      661 aaagataagt tgatcaatat gatatatgtt ttagaattat gccgatttgt tgagctttgc
      721 gttaataatc ccgcagcata ttctcaaaca ttttttgcca cagatggcaa agtttacaag
      781 caaagcgatg ttataaaagc ggttgccaag gctttgaata agaaacctgt tactattaca
      841 gttccagaat ttttggcttg cctgattttt tatgctggtg atttatatgg tcgtttattt
      901 cacaaagaag tggtaactaa tcgagaaaaa atgaaagaaa ttatggcaga tgcctggtta
      961 gcagataccg agaaagcaat aaggatatta ggttggaaac ctacaaccaa tttagaaaac
     1021 aatttgaagg agaccgctaa gtgttatcac gatttaggtt ggttgtagtt tttacccttt
     1081 ttactattcc tttttttgcc cagaaaataa caactgcaga gccaactaaa gttgatactt
     1141 tagagcttgt agagaaaaaa gttactaaag ctgaaattga aaattggata aaagcaaatc
     1201 acctcgacac taatgaacat attttcggtg catcaccttt acatatagac tacggagata
     1261 tatatcataa tggctatttt tatgtaggac gctctgaaat gaatatgcca gaaagaaatg
     1321 gttttgaaac tttatccggt attttttctc ctgctcttta ttatgggtat ttcaattcct
     1381 tatatcaggt taaagaatat cagcgcacag taaattatac aaacaacagt tatccttttc
     1441 cgcctgttct aacatctttg aaagcaggat tgggagatta tgatcatcat tatgcgcgca
     1501 tcaatttatc taaaaatgag cttttttctt atccctatct tgaatatcag ggtgattttc
     1561 tggttcaaaa tggttattgg acagatatta cttctactga aacttctatg aaacatcgcc
     1621 tttcccaatc aggaaaatta gttacttggg agctcgaatt tgccaattgg aaaaaagata
     1681 ttgctatgaa tgaactgctt cctgtatact ggcaatctac gaatttcaca atcaaccata
     1741 acttacagca gttttatgcc tcatttgctt taccttggtt taaactaaaa ttactacagg
     1801 aatccgaaag cgcaaatact aatctgtttt acaaagaata taatcgtaat tccactcgtt
     1861 tacaattttc tgctcagcaa aaaattggct ttctgcaaac tgaagaacta tatgaacata
     1921 actggaataa aggtatttct tgtttttgcg gattttataa tgctgaagaa taccaagata
     1981 agctcgggtt tggtttccag ataaacaatc cagtaaaaat caatcttacc gcggactgct
     2041 tagattggaa acgctacagg ttatttgcta atgctgatta ttctcttaag aactttaatt
     2101 tgggagcaac ccatgaacac aaaatgggtg atgattcctc tgttttgaaa gtgaaagata
     2161 tttttaatgc tgacggagaa ttggatttaa tagatatctc tgtttcgcag gagtcatcgg
     2221 tttattgtag ctataacctc ttcaatatta agccattagt ggcagtaggg acaagagaga
     2281 ttgagcagga agcagaaaat tcctggttaa acataaaaaa acagcaacct tttctccgcc
     2341 tggcgatgga tgtgaattat agctggaaaa attggttgat aaccgcaaga ccggaatggg
     2401 ttatcagtaa atattatcca tcgcttacag aaaatccgga ataccgcttt catagtgttc
     2461 aacagataaa atatatatta ccctatgata atgcagttgt agtaggttgt gatatttatg
     2521 gccattcaga atattatgta gccaacgcgc taaacccata tttgattgaa agttccacaa
     2581 tgctggatgc ttgggcagga attaatgtag gaaatttatt tgaattacgc actggattta
     2641 aaaatgttct ttcctcttct ctatatggaa catatccagt tccagtttct gtatatatag
     2701 atgttttatg gctctatttg aattaagaat agataacgga taactggatc gattggatta
     2761 acaaggttta tttcaataga agatttaatc tgtatgaact attttttaat ctgagtaatc
     2821 aagataatct gtgagagggg ttatatatat gaaagttacg agtttaatat ctatcaatgt
     2881 tttgatgttc ttaatgaaga ataaatagca taatagaacc tgcttgacaa aaaaaggact
     2941 cttatattat attgaattca tcctaaaata ggggttaaat aattaaaaat gaaattattt
     3001 gaaacacatt gtcacctgga tcatcctgat tttgacagcg atcgagaaag cttaataaat
     3061 aaatgcttta gcagtggaat agaatacatt attaatgttg ctttcaacaa agaaacggcg
     3121 ttcagttctt tggggctggc caaaaagcac ttacatatct tttccacagt tggttaccac
     3181 cctcatgatg ctattgattt tgatgctgag atagtaaaaa aactggcaag agagaaaaag
     3241 gtagtagcca ttggtgaaat cggattggac tttttccgaa acttatctcc atttacagtg
     3301 cagcgtgaag tttttgccaa tcaagcacat ttagctgtag aatatgattt accggttatt
     3361 gttcatgatc gagatgctca tcaggaatgt tataacattt taaaaaatga gaatattaag
     3421 gaagcggttt ttcattgttt ctccggtgat attatttttg cgcaacaggt tttggatgca
     3481 ggttggatga tttcttttac aggaaatatc acctatgcta atattcactt agatgatgta
     3541 attagattag ttcccttgga tagatttatg gtagaaacgg attgtcccta tttacctcct
     3601 cacccgcata gaggtgaaag aaattcccca ctatatttac ccttagtagt agaaaaaata
     3661 gctgaagtaa aagggatttc tcctaaggaa atagcagaat tatcatttgc caatgcttat
     3721 agatttttcc gtattcctcc cgatccagtt aaggctttac atcacaaatc tagtcataaa
     3781 aaggttaatt aaaactttaa gctgcaaaaa gatatggctt taaagttaaa ggatatagaa
     3841 tgaaaaaagc agttattatt atctgcggag caatcttatg tatatctctc caggcaggaa
     3901 actccatatt ttcgttttac ggttttcccg ttcataatta tggtagagat atttacagtt
     3961 taggaatggg cgatacagga gccagtgatg ttttcagaat caatactggt tatgccaatc
     4021 cagctatgca caacagaaat aactacactt tattttccac tggaatgaat atgggttata
     4081 caaaatatca ctcacaatat gagggaatag aaagaacttt tagagatgat tctttagatt
     4141 ttccttattt taatattaat attccaattg ggagaaatag gttaggtgtt caattcaatt
     4201 ctcttgcctc tggattagta accaattatc actatcttcc cgacgggtcg gttgaatctc
     4261 aaaccagcga taaatatctt tataaggcag atttgattta cagttacagt tttagtgaag
     4321 tgaacctggg aattagtgcc aactactatt tcgggcataa caaacagtat ttttcacaaa
     4381 ctggaaacta ctccaatttt gacactcgtg aatcaataat aatggatttt aagaacccca
     4441 gcataaccgt tggaactatc aaattatttg ataaatatag tgttggagct cattattgtg
     4501 cacctgttac tttgaaaggt gagctgattc gtagcagcat acacaatact gaacctgcag
     4561 aagattataa atttaaactt cctgaacaat attgtctaag cgctacagcc ttgccgtttg
     4621 aacaattcaa ggtggctgct gatattaatt atgaaccctg gagcacaata tcagacgact
     4681 atcgtgattg ttataagatt gggctaggac ttgcttacga gccaaatatg gaagcacata
     4741 aaaatatttt tatgcgtttt ccgatgaggg tgggtgcttc tatgcgtcaa ttatattttc
     4801 gcgataaaga tggcaaagat attgatgaag tatcacttag ttgtggattg actcttcctt
     4861 taaaaaatga aattgataga attgatttag gatttcaata tctgaaacgc ggtgatttag
     4921 ccaccaataa tctcagcgaa aattcttata tgttgatgat tggatttact ggttttgata
     4981 ttatcagtaa agctcccgat agaacagctc cgcgtaatat acctgtaaaa gaggaagttg
     5041 aatgaaacca gatgaaccta atagtaatat ggattcttct aatgatatat taaatcaaat
     5101 actaaaatct cgcaatttat cgcagcagga tttacaaact aatcttaata tgcttcctga
     5161 tgaagctctt tttgcaaata tagataaagt tgaacacagg attcgggcag ctctctatga
     5221 gaatgaacct atggtaattt ttggccatga tgatcctgat ggtataacta gtacttatat
     5281 tttatataac tttcttaatt cttgtggtta ccaaaaacat cgttattata ttcccaatcg
     5341 caatctggag ccgcatggca ttcaagcagg attcatagat tttgtgcgcg aaggtaatta
     5401 taagcttata gttacagtag ataacggtat tgcatctttg gaaggagtta aacagcttaa
     5461 tgccttagga tgtgatgtta taataactga tcatcatatc gtgcaaccag aggttcttcc
     5521 accagcttat gcaattatga atcctcagct tcctcaatgt gattatcctt tcaaacaact
     5581 tgctggagtt ggagttgttt taatgttaat tcgttatttg ggaaacaaat gggagcatcc
     5641 cattgagcca gcaagttatt tttggacggc tgttggttct gttgccgaca aagttcctat
     5701 gataggaatg aatagaatat tagtccgtta tgcattagag catttttatg aaattcagga
     5761 tgacacagtg gaattcttat tgcgtaatta caatcgcata aacacattaa gcgatattaa
     5821 cagttttctg caatatacag ctcatctttt agcaaatggg cgtgatgata aaggcaggca
     5881 tactgctttg cgctttattt tacaactttc agatgcaaaa gcagatcttt ttcaggagct
     5941 ggaaaaccaa aaaaacaaat gggatagcga tttaaaccga gtattcaatt ttttggatac
     6001 cttagctgct gattttgtag gcaactggtt tgtatattat gatgatgaag atattattcc
     6061 ctatcccctt ttaggtacag cagctactta tatattaaac aaactgaata ttcccacttt
     6121 attgcttaaa agccataatg gcgatactgt ttgtgaaggt agatgcagtg aaggttttaa
     6181 tattatggat gccttcaccc actgtaaaaa gtacttaaag caatttggag gtcatcctaa
     6241 agcagcaggt tttacgatgc aacaggaaaa ttatgatact ttcttggatt gttttaataa
     6301 tttcctcctg aaacactttc atccttccca tacggcatct acgaaatatg atgctgtggt
     6361 agcagccaaa gatattaatc gggaaaattg gcagaaactg gaaattttac taccctgggg
     6421 acagttgaat ccggagccaa ctttgttaat tcgtaatact tctaaagctg aaataatgaa
     6481 ttatgttgct ctggataata gtgggcggga aattcccaac cagggcaaag gcgatatttt
     6541 ggctctatgg aaagcagcta atatgattaa agttttatcc tggcagcaaa cagactaatg
     6601 atcaagcttg ctggaggcac agacatttga tacaacttgg tgaaaccctt aggaaatcta
     6661 ttcatttaag cagtttaatt attccgctgg gatatagata tctgctttcc ttcaatcgtt
     6721 ctttgggctt ctcgattttg ttgattgctt tcactatcag tttagtaata gaattttacc
     6781 gtttctggca aaagggattt cgtaaaacct ttcaccgcat atttggcatc atattaagaa
     6841 agcacgaatt taaggatttt acaggagcaa cttatatgct ctttgcggca gtgttgtgtg
     6901 ttgctttttt ccctcctttg atagcttcct gcgcaatggc ttttttaaca ataggtgata
     6961 cttttgctgc attggtaggt ataaattttg gtaaacgctg gtatattaag cagaataaaa
     7021 gtttggaagg tagtttggcc tgttttgtta gctgttctgt ttttggtatc ttttggttgg
     7081 gaagtccttt tttagctgtt ttgggtgcct tagccgcaac tttggctgag ctaagtaata
     7141 ttcccataga cgataatata acaattccca ttttttcagc tttggtgatg actttgctga
     7201 gtattgtaat ataaaaaagg tagctatgaa attatccttt gttattccgg ttttaaacga
     7261 acaggattct cttgttcagc tatattctga aattatacaa aactgtaatg gctatgatta
     7321 tgaaatcatc tttattgatg atggctcaac agataacagt tttaacatta tgagccagat
     7381 agctttaagc gatatgaaag tgaaggtggt acaattcaga tgtaattttg gcaaagctgc
     7441 tgctttacaa actggtttta atcttacttc aggagatatc gtttttactt tggatggtga
     7501 tcttcaggat aatcctattg aaattcccaa ttttatagcc aaattggatg agggctacga
     7561 tctggtttca ggttggaaac gcaaaagacg cgatcctatt tataaaactc tcccatccaa
     7621 actttttaat ttcgtaaccg caaagacctt tcatcttaag ctacgagact ataattgtgg
     7681 ttttaaagca tatcgtcatc ctttggaaaa ggagattata ctttacggtg agatgcatcg
     7741 ttacattcca gttttagctc attctttagg atataaagtt ggcgaaattc cagttgctca
     7801 tcgtccgcgt aaatttggca agagtaaatt tggaaaagag cgttatctgc gtggtttttt
     7861 tgatctttta actgttaaaa tgattaccca atatagcaaa agtcctttat atttatttgg
     7921 acggatcgga attgcttcca cctttctggg tttgattatc agtatctatc tggcaattct
     7981 gaaaatcttt atgaatcaac ctctctccaa tagacccctt ttgttcttgg gaattttgct
     8041 tatcttgggt gggttgcaat ttatctctat gggcctaatt tctgaactta tagtaaatcg
     8101 tttccgacag ggaaaccgga cacctgtttc tatcgctcaa tatgtaaata tagaccaaat
     8161 tgaatccaaa ccctatcaac aaactaactt acccgaagta aatgagtaag ctggaactct
     8221 tttttttaag tcgttatctg aaagctccca aacgtaacct attacgattt agttttgttt
     8281 ttatgatttt ggggatcatt ctctccgtgg gaatcttaag tgccggactt aacttatttg
     8341 aaggttatga acggactttg aaagatgttt tattgggagc ttttccgcat ctgacaataa
     8401 gtaaagataa tctgggtttc atctcccatt ccgaaacaaa gaacctactg aataaaatgg
     8461 ctaattgtaa agaaattcag caagttactc ctgtccttag ctatcctgtt atggctgcag
     8521 gtaaagcaaa agtgagagga gccactttga atgcttatga ttttagcaat aaaaaatctt
     8581 tccctcaggc aaagtacatt caaaaaggta aaaaaatacc agcttctcaa gaagttatca
     8641 ttggcaaata tctggcaaag gaattaggaa aggatattgg cgatacttta aagttagtat
     8701 ttactcgttt ggataatata tctgcattag gcatttttcc ttcggaatac tatataaccg
     8761 ttgtaggtat ttacagcagt ggattttatg aaacagatcg ctctttaatt cttgccaata
     8821 ttaccgatgc agaaaatatg cttaattcag catcgggctt cagtaaaatt gaaattcgtt
     8881 tggatcctaa atatatagat aaagcaccgg aaattgggca aaagcttcaa gaaatagttg
     8941 gtaattacta ttcagtatat ccttggcaaa cctttagtgc aggtttatta catttggtaa
     9001 ctatggaaaa gtggctaatt tttattatct tctgtttttt agttttaatt gctggaatta
     9061 atgttatatc tgcggtgtca actatcattc tggataaaaa aaatgaaatc gccgtgctta
     9121 aaaccttggg tgccaattct acatctataa aaagagtcct tagtttccag gttggacttt
     9181 ctgctctgct agctattttg gcaggacaaa ttttgggagc tcttctttct tggggtatag
     9241 aaaagcagaa cttttataaa ctaaagggtg atgtttattt tattgacagc ttaaatacaa
     9301 gcatagcacc cttaaatcag ttgatcatct ttattgtttc agcagctctg gtttttattt
     9361 gtatatacta cccccttaag cagatagata agctggaaat tattgaattg ttacgcaacc
     9421 cctgatggat tataatataa tataacacta actaattttt ataaaaagga ttcgaaaatg
     9481 gtctgtttag caggttatga tctctgcaaa agttaccttg atagtaatca aacaattaat
     9541 gtgcttagaa aagccacaat tgaagtaaaa gaagccgaac tggtttgtat tacaggacaa
     9601 tctggctccg gaaaaagcac tctattacat cttttaggtt tattggattc cccagattca
     9661 ggaaaagtga tgataagcgg gcgcgagata aattccacta tgccagaagc agcttcaatt
     9721 cgtaatttag aactcggttt tgtttttcaa tttcactatc tgatagaaga tctaaatgcc
     9781 caagaaaatg tagctttacc gatgattatt tctggcgtaa atagttccca agcaataaaa
     9841 aaagctgcag aacttttaca agctctagat ttagaagatc gtatcaacca ttatcccaat
     9901 cagttaagtg gcggtgaaca gcaacgggtt gctcttgcta gagctttaat aaacaatccc
     9961 aaaattgttt tggcagatga gcctacagga aatctcgatc ctgaacatag tgccgaggtt
    10021 tgggatatga ttattaaatt gaataaagag cgcggccaga cctttgtgat tgtaactcat
    10081 gatattgtta atgcctctaa agcaaaagta atctataatt taagtgaagg caaactggaa
    10141 aaaaaatagg caagattaag tatgccaaga tcaaagaaat tcctgatatt cgttttaatt
    10201 ctgttgttga taaacacagg attctttctt gcttggtatg catttggttt aaataatttt
    10261 ttcaaaaact ctgtggctaa gtatatcggg caacttacca aaggcaaggt tactattgaa
    10321 gagttgcatt ttagcgatcg tcaattattg gctaaaggag taaattatac ttctgctgat
    10381 agcagtttag ctataagcat taatcgttta agcgcacgct ataatctaac tcgatatctt
    10441 ctctccggtt tcaaaaccaa taagttggtt aatgacatag aaatatacaa gccagttgta
    10501 cactatagtt accaatataa gccccaaccc ccgaaagcaa aaaaaccgct tgtccttccg
    10561 aattttgcaa agtactttaa acatcttcag cttaataatg gagctgcagt tttgtccttt
    10621 aatatccctg taaagattac acaagagggc aatttaacta tatcagagag tttatccaat
    10681 ataaatcttg aagctgataa taaatccaca acgaatgcta caattaatgc cctcacttcc
    10741 aatggtggaa aaatatctgc taagggttta ctggataaag gaagaatagt ttctgtagat
    10801 ggagaaatat cagcatataa acctctgttt gtctctcatc ctgatttgca aaatttgcac
    10861 acagaaatat cgttggttgg ttctttaaaa caggattctt tagcagcacc tctgaaatac
    10921 aatgcagaag ctcagatttg gggaacagaa acactatttg ccaacaatta tccagtcctt
    10981 attccctatt tatgggttaa aacagatggt aacaatttaa aattatccct ttccaaaagc
    11041 aatgttggca gcagcgatct tgctgcagaa gttgcaatca gcaatttaag gcataagata
    11101 aaatttgaac gggtagatat ttcggctgat gtagatttgg caatgatcca aaaagacctt
    11161 aagggaatgg tgaatgctaa tattatagga gagggtacaa ttgataaacc agaattggag
    11221 cttagcttag tttctgaaca agctgcttac caaaattaca cttttaataa atttaatttt
    11281 agtggaactt atcaaaacga cttgctggat tttatcattc ctgagcttat ttttggtaat
    11341 cagataatta acttaacagg tagttttaat ccgctaaata tgatattgga tgctaatctg
    11401 gaaactaaac ctatcaataa aaaagctcaa ccttatttag caactgccaa attggattta
    11461 tatgccgaat tatggggtaa atatccttat gtggatgcaa ctatccacca gcttgatttt
    11521 gcttacgagc ttgctaatat tgaaaatgta agtggaaaaa taaaactatt gcctcttcct
    11581 gatgtcaacg atctatattt agatgccaat ttatcaagta gtaatggatt taatattgaa
    11641 gtaataggag atgttcttga tcgtaatctc ttagtagatg ccaaatttga tgatctttct
    11701 attgcagaat tatatgcaca gccaaccttg gtaaatctga atcccggttt gaagggtgaa
    11761 ttgaaagcta ttattaccgg tgataatatt gttgtgcaaa cccttactgg tttaactctc
    11821 gataatccag ttcccgttga tcttacttta gatgccttgg gtaccatcaa ttataaaatg
    11881 aaagatgcca gtatttatgt ttcaggaaca gagggaattg taaacaatca gcccctaaac
    11941 ttcgaactag ccgctatgct aaaagacaac attgtaacct tacaaggctt gaaagttaat
    12001 gatctaatca atgttagtgg agcaattaat ctggcaaact atgcagatat gaatattggg
    12061 ttaaacatca acaacctaaa ttatttggat gtcgttcgct actatcccaa attagatgtc
    12121 aatcttccag aatttaatgg cttgaatgtt tttttagaat ataatcggga taatttacaa
    12181 cgtttacgag ctaatgtttt cttatcggat atagaccttt tagcagttac tcctttttct
    12241 ctggaattgg gaatggaagg tcccttgaat aacattcttg tatttagcac aattaaaaat
    12301 aaagaacaaa atattgtgga tatgacaggt tatgttagtc ttactcctga gctgaatgtt
    12361 aatttggatg cagcttttaa agacctccat attcaagatg ttcttatcaa ttctcccatc
    12421 tatggaatta tagatggaat ctcaggaatt acttggcata aggtaaaaga acaaaaacca
    12481 gaattagagc tgagagcaga tttaatttcg cgagatattg ttcttggtaa ctatacaata
    12541 gataaagcaa ttattaaggg taagcaaact cctcgtaaaa tgatcgtaga tagtttattt
    12601 gttaaatcta caggattatt tgaactttcc ggttccggag caattaacta taatgctctc
    12661 tataacgagt ttttcgaggg tcctgaacat ttgaatttac gggctcaggg tgaacttttt
    12721 acttggttga atggtcttac tccctacatc caagaagcaa aaggttcatc atctttaagc
    12781 gcaagtatcg gaacgacaga agaacaattt atggtttcca ctggaaaaat aaatatcagc
    12841 ggaggctata tccagattaa agaccaaaca gaaccctgct ccaatatcaa tatccgtggt
    12901 ggattcgaca aaaatagagt ggttattgat tatggacaaa tccaaatggg tgaaggcaaa
    12961 ctagtcttca ataatatctt cgaagtcgat aatagcgatc atattatgct gggattcctt
    13021 gatttaggta ttttaagatt gatggtagaa gaacctggaa ttatgattaa tgttccaaaa
    13081 tatacaactc cccgttctct aaccaaagta attttaaaag gtaataacag ccgttatgca
    13141 actattaaag gacccttcga ccaaatgaaa atcagcggag aggttatagc ttccaatact
    13201 aatgttttgt accctccaga tacggaaaac ttattaactt tagcaacctc tatgcgaggt
    13261 acaaataaaa aaagcagttc agaaccatct cccttaccct ttaccttaga tgtaaagctt
    13321 attgcaggag aaaatgttaa gtatgtaacc tatcccgcaa aattaagagt tgttccaggc
    13381 ggctatttac atttaatgta tgatgggcta tctttcattg cagaagaggc atcattctct
    13441 tctgaacaag gaaccgtaga tatctttggc accatctttc aggtagataa agtaaatcta
    13501 accttagttg attcccaaaa tttattgggt ttggaaggga tattttttaa acgcgctccc
    13561 gatggttcta tgataactct tacagcagta acttccaacg at
//
LOCUS       DAOSTH010000045        13307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_4718_length_13307_cov_13.224193, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000045 DAOSTH010000000
VERSION     DAOSTH010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 13307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13307
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4718_length_13307_cov_13.224193"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="PLB65_06005"
     CDS             complement(<1..481)
                     /locus_tag="PLB65_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HOQ78118.1"
                     /translation="MKIRYLIVSWTDVLLEPRDIPKLRGFFVNQFPEDHIFHNHLPGF
                     GYNYKAPQIQYRVMEKHPALLAINDGIDIIKKVFLEVDKLEINGKTLVSNEREISLKE
                     DDFGLTENYYTYFFASPWMALNQENYKDYNRMNAFQRNHKLKTILKNNLKTLSKAFNY
                     "
     gene            1060..1911
                     /locus_tag="PLB65_06010"
     CDS             1060..1911
                     /locus_tag="PLB65_06010"
                     /inference="COORDINATES: protein motif:HMM:NF018184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOQ78119.1"
                     /translation="MPNEHKLDKAIRYIEAGLKTRLILEDIAEQANMSAWHFHRVFLA
                     STGITVGEYIRKRRMSEAAKELVFTEKPIKQLASEYQYESQAAFTRSFMGVYRITPGK
                     LRRQLGPLQYFNALLPQTKGEKMLTPQIKHKDAFRVVGIACQNTMKNNNIPSLWNEFD
                     IMICDKITECLNPGTAFGICFYQDQSVVNEDTPFTYLAGVEFPLEKAVPEGLEYRDIS
                     DADYAVFEHCGSLDTLSQTYSFIYKEWLPNSGFQRKKSDDFELYDHRFQFGTEKSIME
                     IWVPIEK"
     gene            complement(2142..3137)
                     /locus_tag="PLB65_06015"
     CDS             complement(2142..3137)
                     /locus_tag="PLB65_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HOQ78120.1"
                     /translation="MLKKTLYLLALCLLISSNLSAGSKKPVWISDPYSQYPAHRYLCS
                     VGMANDLKSAKNDALKNIAGFFETRVTAVTKSMESESNSASGSQINSSITTSVKAISE
                     GTLKYTEIKATWQDPTTKTYYALAALDKTAVQALYEKEIVESEDYIANCLFKTDDPLI
                     RFAKLCQAIDKHNATQNALVYYNALAGESGSFLRPAFSMQELKAMRAETAKEIPIKIL
                     VVGDESAYLETALRNVIRQLGFSENGISENTEYELSATLETDPQKTIGKQIYQPLRIN
                     ISLKKGDNLLFSYVDKTEQGGPTIEEANSRSIKVLAKNIENKFNKEFTQYLLSIE"
     gene            complement(3228..3944)
                     /locus_tag="PLB65_06020"
     CDS             complement(3228..3944)
                     /locus_tag="PLB65_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78121.1"
                     /translation="MRLSRILLILAILLSMLSLHTALDAKKKKKEVEIKLYLDDAQLE
                     NPEYYNKLPVLSLESFATALLTPKTTNFKIINHNYQIARADSKIAEEMIAVSLGMRQE
                     FVKVEAEGKSLLSGNLEGSEAEISEAMKTKLEDLKNNKAQLSESQKVYFLSGFIFLTS
                     SIIREKIVLESTKMFVNEAKNAKGLDAVFYVKDIPYATNVVVGLPTLLTTQMKTISEF
                     ITIAKTQNIDIPEDVTASLF"
     gene            complement(3958..5076)
                     /locus_tag="PLB65_06025"
     CDS             complement(3958..5076)
                     /locus_tag="PLB65_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78122.1"
                     /translation="MRKLLTIILLSVTMLFSVAYLEAAVKVKVKATVETKKNKPKPEE
                     IKAAVNNAKILAVKKYASEKDTRKRELVNELMPDIEKNIDNYILEINYLNEGNKTNGI
                     WEQLLEVTIDDVQLELLINQKMKDVMEMAEPTYLSFVYVAREVAAVESQGQTIKKDLS
                     TKSDVSASGKIATSGDTDSNLSDPNKISTSSQAEVNIDKSISREGSMVYSQSVVNASE
                     GIYYRTFNPGEIDTKVTEIFNKANFEVVPAYEVGILPEKFAYDFAALNEISSSTQKEA
                     TDIAREAGLDFLAVAMLDVGREEIDPVSGGSKVYVKVNGYILDLRKKFAVKVCSAGPY
                     QFSGIGSNPSVAKTNALIEAAKNASQDLVDQLRVKLGN"
     gene            complement(5061..6152)
                     /locus_tag="PLB65_06030"
     CDS             complement(5061..6152)
                     /locus_tag="PLB65_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78123.1"
                     /translation="MRKNVKIAFIFTLAAGLLFANQDVKYAGFCFSGAYADISKNYPY
                     TQKMLQDSSSAQSYLDKLFFDYFSANSELGNFTLDIHNQTKATLSLAIALNREEVAFE
                     YYDNETKAIYNLGCTVFIMDFADLTILQSYPINVTYIDLYKGEPDQQTIIQTIKKLYS
                     NYILQKLMSNQKNIYIRSSGSLTIKVANVNFQEEALPFLGSYKTEPEIETYAAIVAQH
                     LTEALAFKMNLTVLPYSRSNLSYKMSLSFSDASIQNFTIPAASYDIDITVKRFLKKLY
                     KETAAERVDLYGVELEMRIYDAEFNQEYWKQTIQNGAAKQTVVGQIIGNEFYKYNEIL
                     LLTLSDKIPATLKEDKKLMKGILKKCANY"
     gene            complement(6433..7272)
                     /locus_tag="PLB65_06035"
     CDS             complement(6433..7272)
                     /locus_tag="PLB65_06035"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOQ78124.1"
                     /translation="MPIVAKVFEFEISEQDLERECAKVSITERKECLQNALKRLIDRY
                     LLLYKAIESGITISDAEYDKAIWELLDEENPLDLWSTSLDQLTPKEMENLLKERLMIN
                     KYINSFFSHDTPVTKDKVKEFYDEQKEYFLKAEQVRCSHILISNTKKDAKALAEEIRK
                     KIHNAEDFGYYCKEYSDCLSNSSCGDLGWFSRGIMIPEIEEVAFALKVGEISQPFPSK
                     YGYHILLKTGHSEKDYIPFEEIKDSLYARVQQIEKEYLLIRHLAELHKQYAAKIIIYK
                     EYL"
     gene            complement(7247..8191)
                     /gene="miaA"
                     /locus_tag="PLB65_06040"
     CDS             complement(7247..8191)
                     /gene="miaA"
                     /locus_tag="PLB65_06040"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOQ78125.1"
                     /translation="MIPVITIEGATASGKSALAIALAESLCTEIISADSRQVYRYLDI
                     GTAKVAKAEQQRAKHHLLDIINPDETYNAGSFAADAEKIITSLHSEGKIPIICGGTGL
                     YIKALLNGLFTLPPLPTKIRYDLKQRLKEEGLTSLYSELLNIDPEFASKISENDTQRI
                     LRGLEVFSGTGIPLSEHWRKQKETGKYNAFRILIDMPRPQLYTRINERMEKMLAEGLL
                     QEIENLFTMGYTDHSPGLQCLGYKEFLPYFREEASIEDCAIIAAQHQRNYAKRQVTWY
                     RKYTFDLTIDSSSIILYNIVTKIKEGLKKKNANSSESI"
     gene            complement(8188..10080)
                     /gene="mutL"
                     /locus_tag="PLB65_06045"
     CDS             complement(8188..10080)
                     /gene="mutL"
                     /locus_tag="PLB65_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOQ78126.1"
                     /translation="MNNIHILSEDVSNKIAAGEVIERPASVVKELVENAIDAGANNII
                     VVIENGGKDLIRVIDNGCGMNPDDAVLAFERHSTSKIKTVDDITHIGSLGFRGEALPS
                     IASVSRLHLVTRTAEDEMATIVEFTNGKLINVTKTSANIGTAITIRGLFKDLPARRKF
                     LKTSPVEARHILKYIHYQALVYPEISFRLVMDDKEKLNYIACVDRKQRIEEVFGIGFF
                     EDDVIPVEGKDNNYSISGYIFGLEDRSNRLIDAQYTFINGRYIFDKTVRHAIKAAYEP
                     FILKTRAWQKGTTPPYIVFLEVPPEQIDVNVHPAKLEVRFRENNLVHTLVLETLTKAL
                     RAYEDSKFASARSKFRGGVQGTKVTSLERDVFVSNIEVPKFSAYKKEFGALFQDDIFT
                     SESSPTAQKDIPIFNPGKESDEILEIFADQPNDKLKYDILLKNEEDYINPWQFHNTYI
                     FIQVEDGLVIIDQHAAHERIIYEKLLQRTGGAPAMRQKLIIPLVIDIPPYIASDIKEL
                     VEENLELLEKVGFTLKKFSGDSIVIEEIPAELSDFASGQIFIEILKQLEQEMEIATDF
                     KEGLAKSIACKAAIKANHKLTRKEMLNLINDLFACKLPYFCPHGRPLILKIPVSDFEK
                     KFKRIV"
     gene            complement(10090..11751)
                     /locus_tag="PLB65_06050"
     CDS             complement(10090..11751)
                     /locus_tag="PLB65_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HOQ78127.1"
                     /translation="MILDIRLNGIKHSLIEVDKPLPLTEILKRTHIDRSQILSYKINH
                     REYVNEDYIPDRETLIDCITYLHPEGYRIYQDSTIFIMCKALHTLMGTEPSLVVEHSI
                     ADGVFCEVFNSQTFTEEDCSRLIAEMQNIVENNLPIEKITVKTDEALDIFSALGRNDV
                     IKNIKYHYQENVELYRCGKYYDCYNHPLTDRTGKIKNFDIVYHSPGFILRFPSKDENM
                     EIPTFQLPYKLFARHQEHDKWLDILRVHNIIDINKLIDHYEISEFIQVEEALHEKKIA
                     EIAADIVTQKEVKLILIAGPSSSGKTTFAKRLGVQLQACKAKPVVIGMDDYFLPRTKT
                     PRKPNGEYDFETIYAMDLEYLNMQMNQLLDGEQIELPRYDFTRGIRRRSNTFVKLGSN
                     SIIVMEGIHGLNEILTASIPANRKLKIYVSALNQLNIDNHNRISTTDCRLLRRIIRDH
                     RYRGYSAEETLMRWQDVRDGEDKYIFPYQENADYMFNSSLTYEIGVIRKYAWKLLLGV
                     SPSSSAYMEAKRLSSLIASCKDIADTLVPYNSIIREFTDGSIFRY"
     gene            11863..12816
                     /locus_tag="PLB65_06055"
     CDS             11863..12816
                     /locus_tag="PLB65_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HOQ78128.1"
                     /translation="MTSITKKQYLRRETGRLIGIIFASVFFAMGYSWFLLPYNMAPGG
                     VGGLSQILYYFFGIPNGVSMILINIPLFIVSFIFIGKSFGSKSLYGMFVSAIMTDFLS
                     FSSLHKIGIIKDLTPYTHILNGRKIYAMLPPEDMLLSAIAGSVLLGIGLGLIFRFRGS
                     TGGTDIPVALIKQKANLSIGTGYYIVETGIILLVAIVLKSPKLLIWGYLNLFITTKIT
                     DLVSEGLPYIKGAYIISEAVDEISKEVFEKLERGATFINGVSGYHQRDIKILFVVLNR
                     RQVPQLTDIVKDNDPDAFMIIMDVYDVLGYGFKSRSINLSE"
BASE COUNT         3979 a         2366 c         2496 g         4466 t
ORIGIN      
        1 aatagttaaa tgccttggaa agggttttga ggttattttt caggatggtt tttagtttat
       61 ggttacgctg aaaagcattc attctattat agtccttata gttttcctgg ttcaaagcca
      121 tccagggaga ggcaaaaaag taagtataat agttttcggt taagccaaag tcatcttctt
      181 tcaaggagat ttctctttcg ttggaaacga gtgttttgcc gttaatttcc agtttatcca
      241 cttccagaaa gacctttttg atgatatcta taccatcatt gatggcaagc agtgcaggat
      301 gtttttccat aactcgatat tgaatttggg gagctttata gttataacca aacccaggga
      361 ggtgattatg aaaaatatga tcttcaggaa attgattcac aaagaaacct ctcagtttgg
      421 gaatatccct tggctcgagc agaacatcag tccaactgac aattaaataa cggatcttca
      481 ttccatttta cttcctaatt acataataat aggtaactgc catacatcgt gtctctaaaa
      541 gatattttct ttttttatag ataaaaataa attattattc tgtcaaggaa aaaataaact
      601 aataagtatt actctgattt acaagaagtt atggatgctt tataaaatag ggtcgttttg
      661 ctgttttgac ttttgggcaa agagaaaaaa agaactaatg atagaaagca ataagaagtt
      721 acattattat aggtttatta gataccatag ggaacaccgg aattactgaa agaagctaat
      781 aaggcagatt tgttttttta ataattatat tagcaaggat tccccaaggg tgaaaataat
      841 aataggtagg tatggctatc attatgctaa gtatataggt aagcgataca gtacagtaat
      901 tatattctaa caattctctt tctggtttta ggagcacagc aagtagtcgg ctagttatgt
      961 tatagtataa ttccgcaaaa aaaatcaaat ctttctgctt gacttatgaa atgctgaaaa
     1021 ttatagcaat aattatcaaa agagcgttag gagtgtaaaa tgcccaatga gcataaactt
     1081 gacaaagcaa tacggtatat tgaagctggg cttaagacaa gactgatttt ggaagacatc
     1141 gctgaacagg ctaatatgtc agcttggcac tttcaccgtg tatttttggc ttccacagga
     1201 atcacggtag gtgaatatat tcgcaaacgc aggatgagtg aagctgcaaa agaactagtt
     1261 tttacagaaa agcccatcaa gcaattagca agcgagtatc agtatgaatc tcaagcagcg
     1321 tttacaaggt cttttatggg ggtttataga atcactcccg gtaagttaag acgccaattg
     1381 ggccctttac aatattttaa cgcgctatta ccacaaacca aaggagaaaa aatgcttaca
     1441 cctcaaatta agcacaaaga tgcttttcgc gtagttggga tcgcttgtca aaatactatg
     1501 aagaataaca atattccttc tttatggaat gagtttgaca taatgatttg tgataaaatt
     1561 acagagtgtt taaatcctgg aacagctttt ggaatctgct tctatcagga tcagagtgtt
     1621 gtgaatgaag atacaccttt tacttatctt gcgggagtgg aatttccgtt agaaaaagca
     1681 gttccagaag gattggaata tagagatatt tccgatgccg attatgctgt atttgagcat
     1741 tgtggttcat tggatactct atctcaaaca tacagtttta tttataaaga atggttaccg
     1801 aatagtggtt tccagcgcaa aaaatctgat gactttgaac tatacgatca tcgctttcaa
     1861 tttgggacag aaaaatctat tatggaaatt tgggtaccga tagaaaagta ataacaagtc
     1921 aaaacagata tttcagaggt ttgggagata tattaatgtt tcccaaacca tttatttttt
     1981 agtgtttaca gaattgctag ttcatatgtc aagccacaaa attatagttg tagcttttac
     2041 gaggggctta cgccaccgtc gctatcgtta acagtgtttt agcttctggg ctataaaggt
     2101 ataattacat tcaataaaag acccctaaaa tgttcttatc tctattctat gcttagcaag
     2161 tactgggtaa attccttatt gaatttattt tcgatattct tagccagtac ttttatgcta
     2221 cgcgagtttg cttcttcaat tgttggtcca ccctgttctg ttttatccac ataagagaaa
     2281 agtaagttat ctcctttctt taagctgata tttatacgca ggggttgata aatctgtttt
     2341 cctattgttt tttgcggatc ggtttccagg gttgccgata attcatattc tgtattttcg
     2401 gaaattccgt tttcgctaaa ccccaattgg cgaataacat ttctgagggc ggtttccaga
     2461 taggcagatt catctcctac aacgagtatt ttaattggaa tttctttggc agtttcagct
     2521 cgcatggctt tcaattcttg cattgaaaaa gcagggcgca aaaatgaacc tgattcacca
     2581 gctaaagcat tataataaac taaagcattt tgggttgcat tatgtttatc tattgcctga
     2641 cacaatttag caaagcggat aagcgggtca tcggtcttaa aaaggcaatt ggcaatatag
     2701 tcctcacttt ccacaatttc tttttcgtaa agggcttgca cggcggtttt atctaaagcc
     2761 gcaagggcat aataagtttt agttgtagga tcttgccaag ttgcttttat ttcggtatat
     2821 tttaaagttc cttcagaaat ggcttttacg gaggtcgtta tagagctgtt tatttgagag
     2881 cctgaagcag aatttgattc actttccata cttttagtaa cagctgtaac cctggtttca
     2941 aaaaagcctg cgatattctt taaagcgtca ttttttgcac ttttgaggtc attagccatt
     3001 cctactgagc aaagataacg atgggcagga tattgggaat agggatcgga gatccaaacc
     3061 ggttttttac ttcctgcaga taggttggaa ctgataagta gacacaaggc aagcaggtaa
     3121 agagtttttt ttagcataat aatatccata tagcgaaatt acaatcagca agggctgcta
     3181 taaagctatg ctgcttagaa tcttttgtta taaagccaag ggggttatta gaacaagctt
     3241 gcggtaacat cttccggaat atcaatgttt tgggttttag caatagtgat aaattcgctg
     3301 atagttttca tctgagtggt aagcaaagtt ggcagtccaa caaccacatt tgtggcataa
     3361 gggatatctt ttacataaaa aacagcatcc aaacccttag cattttttgc ttcgttaaca
     3421 aacattttag tgctttccaa aactattttt tcgcggataa tggagctggt tagaaagatg
     3481 aatccgctta aaaaatatac tttttgcgat tcgcttaatt gagctttatt attttttagg
     3541 tcttccagtt ttgttttcat cgcttcagaa atttctgctt cgctgccttc caaatttcct
     3601 gaaagaagag atttcccttc tgcttccact tttacaaatt cctggcgcat ccctaacgaa
     3661 acagcaatca tttcttccgc tattttggaa tcagctctgg caatttgata gttatgattg
     3721 atgattttga aattggtagt tttgggggtt agtaaagcgg tggcaaaaga ttccaaggaa
     3781 agcacaggca gcttattata atattccgga ttttccaatt gagcatcatc aagatataat
     3841 tttatctcta cttccttttt tttctttttg gcatccagag ctgtatgcaa agaaagcatt
     3901 gaaagcagga tagcgagaat taataagatt ctggataatc tcatataatt cttatgttca
     3961 atttccaagc tttacgcgaa gctggtcaac taaatcttgg gaggcatttt ttgcagcttc
     4021 aattaaggca ttagttttgg ctacagaagg attggagcca ataccgctaa actgataagg
     4081 tcctgcactg catactttta ctgcaaattt tttgcgtagg tctaaaatat agccatttac
     4141 ttttacataa accttactac ctccagaaac aggatcaatt tcttctctgc caacatctaa
     4201 cattgctaca gctagaaaat ctaaacctgc ttcgcgagct atatcagtag cttctttttg
     4261 ggtggaagag gagatttcat ttaaggcagc aaaatcgtag gcaaattttt cgggtaaaat
     4321 gcctacttca taagcgggaa caacttcaaa attggcttta ttgaatatct cggttacttt
     4381 tgtatctatt tccccagggt taaaagtgcg gtaatagatt ccctcactgg catttactac
     4441 agattgagaa taaaccatag aaccttctcg ggagatactt ttatcaatat tcacttcggc
     4501 ttggctgctt gtgcttattt tattagggtc ggataaattg ctatcggtat ctccgctggt
     4561 ggcaattttt cctgaggcag agacatccga tttggtagaa aggtcttttt taatagtctg
     4621 tccttggctt tctacagctg caacttcacg agctacatac acaaagctta aatatgttgg
     4681 ttcagccatt tccattacat ctttcatttt ttggttaatt agcaattcca gctgaacatc
     4741 gtcgatggta acttccaaca attgttccca aatgccgttt gttttgtttc cttcgtttaa
     4801 gtaatttatt tccaggatgt aattatcaat attcttttct atatccggca tcagttcgtt
     4861 gacaagttct cttttgcgtg tgtctttttc ggaggcgtat tttttaacag ccagaatttt
     4921 agcattatta acagcagctt taatttcttc aggtttgggc ttgttctttt tggtttctac
     4981 ggtggcttta actttcactt tgacagctgc ttccagataa gctaccgaaa agagcattgt
     5041 aacgctaagc aatataatag ttaatagttt gcgcattttt ttaatatccc cttcattaat
     5101 tttttatctt ctttcagtgt ggcgggtatt ttgtccgaca gcgttaaaag aagaatttcg
     5161 ttgtatttat aaaactcatt gccgattatt tgtccgacaa cggtttgttt agcagcacca
     5221 ttttgaatag tctgtttcca gtattcttga ttaaactcgg catcgtaaat acgcatttct
     5281 aattccacac cataaagatc aactctttct gcggcagttt ctttgtaaag ctttttaaga
     5341 aagcgtttaa cagtgatatc tatatcgtaa ctggcagcag ggatagtaaa attctgaatg
     5401 ctggcatcag aaaaggaaag agacattttg tatgataaat tactgcgaga ataaggtaaa
     5461 acggttaaat tcattttaaa agcaagtgcc tctgttaaat gctgagctac aatagcggca
     5521 taagtttcaa tttctggttc tgttttataa gaaccaagaa acggcaatgc ttcttcctgg
     5581 aaattcacat tggcaacttt gatagttagc gaaccagaag aacggatata gatgtttttt
     5641 tgattactca tcagcttctg taaaatgtaa ttggaataga gtttttttat agtttggata
     5701 attgtttgct gatcgggctc tcctttgtaa agatcaatat aagtaacatt tatgggatag
     5761 gactggagaa tagtaagatc cgcaaaatcc attataaaaa cagtgcagcc caaattgtaa
     5821 attgccttgg tttcattatc gtaatattca aaagcaactt cttcccggtt taaagctatt
     5881 gccagagata gggttgcctt ggtttgattg tgaatatcta atgtaaaatt acctagctcg
     5941 gaatttgcag aaaaatagtc aaaaaaaagc ttatctaaat aggattgagc ggaggaacta
     6001 tcctgaagca ttttttgagt gtatggatag tttttgctta tatcggcata agcaccacta
     6061 aaacaaaagc cagcatattt aacatcttga ttggcaaata ataaacctgc tgccagagtg
     6121 aatatgaacg ctatttttac attttttcgc atacctatat ttttaaccat cccctataag
     6181 tacatctttc tttaaatttt cctatagttt attctgctat ttctgtcaag aaaaagatgt
     6241 ttagataaac ttcagcagat attttttagc cccagaaaac accgaataag gtaaagccta
     6301 taaattagat tataacttta agctcccaca ctacaaacta acaactttta tagttaccgc
     6361 aaatataaat cctcaccgcc aaacacaaga tagagatgtt cataagatat ttgggcagtg
     6421 ataactccct tgttataaat attctttata gataatgatc ttagcagcgt attgtttatg
     6481 aagttcagcc aaatgcctaa tcagcaagta ttctttttca atttgttgga cgcgggcata
     6541 caaagaatct ttaatttctt cgaaggggat ataatctttt tcgctatgac ctgttttcaa
     6601 caggatgtga tagccatatt tagaagggaa aggctggcta atttcgccaa cttttaaagc
     6661 aaaggcaact tcctcaattt cgggaatcat tattcctctg gagaaccatc ccaaatcacc
     6721 acaggaggaa ttactaagac aatcggaata ttctttacag tagtagccaa agtcctccgc
     6781 gttatgtatt tttttgcgga tctcttctgc cagggctttt gcatcttttt tcgtgttact
     6841 gatcaggata tgtgaacagc gaacctgctc tgctttcaaa aaatactcct tttgttcgtc
     6901 gtagaattct tttactttgt ccttagtaac aggagtatca tgcgagaaga agctatttat
     6961 atatttgttt atcatcaatc gttcttttaa gaggttttcc atctctttgg gggtgagctg
     7021 atctaaggaa gtgctccaca ggtctaaggg attttcttca tctaagagtt cccaaattgc
     7081 tttatcgtat tcggcatcgc tgattgtgat tcctgattct atagctttat aaagcaagag
     7141 gtatctatca attaaacgtt taagcgcatt ttgcaggcat tctttccgtt ctgtaatact
     7201 aactttggca cattcgcgtt ctaaatcctg ttcgcttatc tcaaattcaa atactttcgc
     7261 tactattggc atttttcttt tttagacctt ccttaatctt ggtgactata ttgtatagta
     7321 taatcgaaga agagtcaatt gtcaaatcga aagtgtattt acgataccaa gttacctgtc
     7381 tttttgcata gttgcgttga tgttgagctg caataattgc acaatcctct atgcttgctt
     7441 cttctctaaa atagggcaga aactctttat agccaaggca ttggagtcct ggtgagtgat
     7501 ctgtataacc cattgtaaag aggttttcaa tttcttgcag taagccctcg gcaagcattt
     7561 tttccattcg ttcattaatt cgtgtatata gttggggacg gggcatatcg atcaaaattc
     7621 ggaaggcatt atattttcca gtttcttttt gcttgcgcca gtgttcagaa aggggaattc
     7681 ccgttcctga aaaaacttcc aagcctcgca aaattctttg tgtgtcattt tcactgatct
     7741 tagaggcaaa ttctgggtct atatttagca gctcggaata tagagaggta agtccctctt
     7801 ccttaaggcg ttgctttaaa tcgtatctta tttttgtggg taagggaggc agggtaaaaa
     7861 gcccatttaa taatgccttt atatataaac ccgttcctcc acagattatt ggtattttgc
     7921 cttcagaatg cagcgaagtg attatttttt ctgcgtcggc tgcaaaagaa ccggcattat
     7981 aggtttcgtc gggattgata atatcaagca ggtgatgttt tgctcgttgt tgttcagctt
     8041 tagcaacttt tgcagttcca atatccagat agcgatagac ctgtctggaa tcggcagaaa
     8101 tgatttctgt gcataatgat tcagctaatg caatagctaa tgcagatttc ccagaagcgg
     8161 ttgccccttc gatagtgata acaggaatca tactatgcgt ttaaactttt tctcgaaatc
     8221 ggagaccggt attttaagaa tcaagggtct gccatgggga caaaaatagg gaagcttaca
     8281 agcaaagaga tcattaatta aattcaacat ttccttgcgg gtgagtttat gattggcttt
     8341 tatagcagct ttacaagcaa ttgattttgc cagaccttct ttgaaatcgg tagcaatttc
     8401 catctcctgt tccagttgtt tcagaatctc gataaatatc tggccactgg caaaatcgga
     8461 taactcagca ggaatttctt caatcacgat agaatcacca ctgaatttct ttagggtgaa
     8521 acctaccttt tcaagcagtt ccagattttc ttctaccagc tctttaatat cagatgcaat
     8581 atagggaggt atatcaataa ccagaggaat aattaatttt tgacgcatag cgggggctcc
     8641 tccggtacgc tgtaaaagtt tctcatagat tattctttcg tgagcggcat gctgatcaat
     8701 aataactaag ccatcttcta cttgaataaa aatataggta ttatgaaact gccagggatt
     8761 gatatagtcc tcttcatttt tcagcaggat atcgtattta agtttatcat taggctggtc
     8821 agcgaaaatt tccagaattt catcggattc ttttcccgga ttaaagatcg ggatatcttt
     8881 ttgggcagtt gggcttgatt cgctggtaaa aatatcatcc tgaaataatg ctccaaattc
     8941 ctttttatag gcagaaaact taggaacttc gatattacta acaaagacat cgcgctctaa
     9001 agaagttact tttgtaccct gtactccacc gcgaaattta ctgcgggcgc tggcaaattt
     9061 actatcttca taagctcgta aagccttagt taaagtttcc aaaaccagag tatgtactaa
     9121 attattttcc ctaaagcgca cttccaactt ggcaggatga acatttacat ctatctgttc
     9181 aggaggtact tccaaaaaaa ctatataggg cggagtagtt cctttttgcc aagcgcgggt
     9241 ttttaaaata aaaggttcat aggcagcttt aatagcatgg cgaacagttt tatcaaaaat
     9301 atagcgtcca ttaataaagg tatattgggc atcaattaat ctattagatc tgtcttccag
     9361 accgaagata taaccgctta tagaatagtt gttatcttta ccttccacag ggatcacatc
     9421 atcttcaaaa aatccgatgc caaagacctc ttcaattcgc tgttttctat ctacacaagc
     9481 aatgtaattc agcttttctt tatcatccat caccaggcga aaacttattt caggatatac
     9541 taatgcttga taatgaatat acttaaggat atgcctggct tccactggag aggtttttaa
     9601 aaatttccgc ctcgcaggaa ggtctttaaa taatccacga atagtgattg ctgttccaat
     9661 attagccgag gttttagtta cattaattaa cttaccatta gtaaattcta caattgtggc
     9721 catttcatct tcagcggtac gggtaactaa atgtaatctg gaaacagaag caattgaagg
     9781 tagcgcttca cctctaaaac ctaatgaacc aatatgtgtg atatcatcca cggtttttat
     9841 tttactggtg gaatggcgtt caaaagccaa aacagcatcg tctggattca ttccacagcc
     9901 gttatctatt acgcggataa ggtctttacc accattttcg ataactacaa taatattatt
     9961 agcaccagca tcaatagcat tttccacaag ttcttttaca accgaagccg gacgttcaat
    10021 aacttcgcct gcagcgattt tattgctaac atcttcagaa aggatatgaa tattattcac
    10081 ttttgttcct caataacgga atatactgcc atcagtaaat tctctgatta tagaattgta
    10141 gggtacaagt gtatcagcaa tatctttaca actggcaatt aaactggaaa gacgttttgc
    10201 ttccatatag gcagaagaag aaggagaaac tcccaacagc aatttccagg catatttacg
    10261 aattactcca atttcataag ttaagctgct gttaaacata tagtcagcat tttcctggta
    10321 agggaaaatg tatttatctt caccatcgcg tacatcctgc cagcgcatta aggtttcttc
    10381 tgcactataa cctctataac gatgatcgcg aattattctt cttaacaatc ggcaatcagt
    10441 tgtagatatt cgattatgat tatcgatatt caattgattg agcgcactaa catatatttt
    10501 caatttacgg ttggcaggaa tggaagcagt aagaatttcg tttaaaccat gaataccttc
    10561 catcacaatt atgctgttag aaccaagttt tacaaaagta ttgctgcgcc tacgaatccc
    10621 tcgcgtaaaa tcatagcggg gcaattcaat ttgttcgcca tccagcaatt gattcatctg
    10681 catattcaga tattccaaat ccatagcata tatggtttca aagtcatatt caccattggg
    10741 tttgcgggga gtttttgttc tcggcagaaa ataatcatcc attccaataa ctacaggttt
    10801 agctttacag gcttgaagct gaactcccaa ccttttggca aaggtggttt tacccgaaga
    10861 agagggaccg gcaataagga ttagtttcac ttctttttgg gtaacaatat cggcagcgat
    10921 ttctgctatt tttttctcgt gtagagcttc ttccacttga ataaattccg agatttcata
    10981 gtggtctatc aatttattga tgtctattat attatgaacg cgtaatatat ccagccattt
    11041 atcgtgttcc tgatgcctgg caaataattt atacggaagc tgaaaagtag gaatttccat
    11101 attttcatct ttggagggaa agcgtaaaat aaaaccggga gaatgatata caatatcaaa
    11161 gttcttgatt tttccggttc tatcggttag cggatgatta tagcagtcat aatatttccc
    11221 gcagcgataa agttccacat tttcctgata gtggtatttg atatttttga taacatcatt
    11281 gcggcctaaa gcactaaaaa tatccaatgc ttcatctgtc ttaactgtta tcttttctat
    11341 gggtagatta ttttccacaa tgttttgcat ttctgctatt aggcgactgc aatcctcttc
    11401 ggtaaaagtc tgcgaattaa aaacttcgca aaaaacaccg tctgcaatag aatgttctac
    11461 tactaaagat ggttccgttc ccatcaatgt atgcaatgcc ttgcacataa taaaaattgt
    11521 gctgtcctga taaattcgat aaccttcagg atgaagataa gtgatacaat ctattagtgt
    11581 ttccctatcg gggatgtaat cttcattgac atattcccga tggtttattt tgtaggacaa
    11641 tatttgtgat ctgtctatat gagttctttt tagtatctct gtaagtggaa ggggtttatc
    11701 cacctcgata agtgaatgtt tgattccgtt taagcgaatg tctaatatca tcttttttcc
    11761 ctgttattcc cattgctaat ggggaattat aatagtcatt tttatcatac caagtattta
    11821 aaaacaaaaa attagtcaag tgatatagag gagagagagc aaatgacaag tataacgaaa
    11881 aaacagtatc tgcgtagaga aacaggacgc ctgataggaa ttatctttgc ctccgttttc
    11941 tttgctatgg gttattcctg gtttttatta ccatataata tggctcccgg aggagtgggg
    12001 gggttatctc aaattttgta ttactttttt ggcataccca atggggtatc tatgattcta
    12061 ataaacatac cactgtttat agtaagcttt atctttatag gtaagtcctt tggcagtaaa
    12121 agtttatatg gaatgtttgt gagcgctatt atgaccgact tcctaagctt ttcatctctt
    12181 cataaaatag gtataattaa agatttaaca ccctacacac atattttaaa tgggcgtaaa
    12241 atttatgcta tgcttcctcc agaagatatg cttttaagcg caattgcagg tagtgtgcta
    12301 cttgggattg gattgggact tatttttcgg tttagaggtt ctacaggagg aaccgatatt
    12361 cctgtagcac ttattaaaca aaaggcaaat ctttcaattg ggacaggtta ttacattgta
    12421 gaaactggaa ttattcttct ggtagccatt gtgcttaaat cacctaaact gcttatctgg
    12481 ggctatttaa atctgtttat tactacaaaa ataaccgatt tagttagtga gggacttcct
    12541 tatattaagg gagcttatat tatctccgaa gcagtagatg aaattagtaa agaagtattt
    12601 gaaaaactcg agcggggggc aacatttata aatggagtta gtggttatca tcaaagagat
    12661 ataaagatac tttttgtagt gctaaacaga cgccaggttc cacaattaac tgatatcgtt
    12721 aaagataatg atcctgatgc ctttatgatt attatggatg tatatgatgt tttgggatat
    12781 ggatttaagt ctcgcagcat aaatttaagc gaataatcct gatcatcacc catcctccac
    12841 ttatgattgt ttaatgagaa taggatgggt ggctattaat ttaggtctat acataatttc
    12901 cttgtagacc actcagatat aactcgtcat ctacctaacc ttcactcctc cccaacgacg
    12961 gtttgaggac gggaggagga ggagaggaag atgagaggag gatgggttgt ttgtttgaat
    13021 catcttgaaa atagtggact gaatttaagg agaaggatat gcgcaaaaag tctacttaac
    13081 aggatgcata ttcaggatag cgatgccttt gaacctaaag ttgcttatat atctgtaatc
    13141 cgtgtaatct tcgtgaactt attctaaaat ccttgttctt aaaatgcaaa tctgcataat
    13201 cggcgtaatc tgcgtgaact tattctaaaa tccttgttct taaaatgcaa atctgtgtaa
    13261 tccgtgtaat cagtgagagg atattttttt cggtggcctc ggtggct
//
LOCUS       DAOSTH010000046        12713 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_4964_length_12713_cov_13.032075, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000046 DAOSTH010000000
VERSION     DAOSTH010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 12713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12713
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4964_length_12713_cov_13.032075"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            664..1389
                     /locus_tag="PLB65_06060"
     CDS             664..1389
                     /locus_tag="PLB65_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOQ78129.1"
                     /translation="MRFDVSYFCHYGNRALKNQDRVIVNGDIVSCGVKHQENIAALFC
                     FVADGIGSLINSENAAQYVLEQIDFLRLRPGLDWCNLIDSFLQNTNLELVKMNHERDD
                     FEDSATTLCGLIYRDKIFIPINVGDSEIWLYRNKELLRITKPHIEDECIPNSPINNYF
                     GSFQAHMYIDFITSIPCLLPDDKLVVTTDGLLKAISKEELIHILEDNAPLKNKINALF
                     YQLSAKDNPDNLGAIFIQVKKDK"
     gene            complement(1394..2635)
                     /locus_tag="PLB65_06065"
     CDS             complement(1394..2635)
                     /locus_tag="PLB65_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78130.1"
                     /translation="MRKIFILMLFSWVVICSAFQVDVFGPEADDFNCANLNQEPASFG
                     TRINEQEKLCLWENGEWNNYSYYVNGLPVISTCMLNADTMLVAMGAMTYSDGIYNFDI
                     HTHTWQLNDWFLLPNFVKYNPNSQLYYVGERDGLYRSTNGHNWSRINSLGLNECSSLA
                     WYGNNIITNNGQIVYYTNDNGLNWHQSSISNLHSFRFDNNGILYAIMDVTSDSDGLWH
                     SNDFGANWVNILYTSALACIGPVFNNNVTLGWRECYEDSSYVGMFRSNGTVSHFNHPS
                     INKPVKQMDIFPYIDTFSFYVLNSDGCFYLTSFLVGIEDPVIPPIPIAKISAYPNPAT
                     AYANIAIYKNSPEPAELNIYNLKGQLVRSIEISRITKESTLNWDLNSFNSTKVAAGIY
                     LLVLRDKAGKPLASNKLAVVK"
     gene            complement(2738..4531)
                     /locus_tag="PLB65_06070"
     CDS             complement(2738..4531)
                     /locus_tag="PLB65_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOQ78131.1"
                     /translation="MPGINCLINAKGITPQNRNTIDLHLQRFGATFPHLQLANFMPCS
                     EVLLTFSQHQGYQVYTESYKGWQIFHELQDNILITQTVTDILEQVENNSISLANLRKS
                     WNMLLHNTYESFFLLAVNPDLHKIIFANDALARLPIYYTQQKDCFILGRDISWVKAIS
                     DQLTLNPLFMALYLSNAYIPGRGTFYQEIDTLASGTFAIYDWLTDDLQMISQPNMRFV
                     EPLAGRAEKKRLNELVETFMDVCVSYRTKLPKLLSLSGGMDSRCVAGALQQKSIDFVP
                     ISFLDFQKEVKDDVFIASQIAELYHKSHNVIQLSLCEPEHYEKLFYLKAGLNYLPVAM
                     FLQYLEKILAQYPQSALFLTGDGGDKVMRYLLPDKELADEKQWLNYWYSQNAIIPTKD
                     SAAIFGIPEKEMDEYLLNHLSTYPTGNYNYKYASAILAERSARWAFEGEDRNRYFFRS
                     ETPFFDFRFYQLAMQIPMEQKKDNVLYYNFLYALSPSLAKLRYARYQWSPAKMRNIFY
                     RILVNQTRKIRLKYRSHKEAINHQQSFAEQDYIINWILEQSQKSIVKEIMPGAKSFLN
                     LEYLQNLSPSQLGTLFTSISVITGGPISPQK"
     gene            complement(4716..5516)
                     /gene="pyrF"
                     /locus_tag="PLB65_06075"
     CDS             complement(4716..5516)
                     /gene="pyrF"
                     /locus_tag="PLB65_06075"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HOQ78132.1"
                     /translation="MGKIVSKSFWYKYQERYNNTKSVLCVGLDSDFNQLPKCVKNAQN
                     PIWEFNKAIIEATLDYACAYKPNLAFYLADGLRGLDALYKTVDFIPDNIPVILDVKVG
                     DIGNTMNAYLTAFFKDLEVDAITLNPLMGKDVMQPVLEKNGFAFVLCLTSNPGASDFL
                     KDGMAEKIANWLQNYPKENFGAVVGATQSADLKKMRNLLQDRILLIPGIGAQGGDLKT
                     VLQQATYSEATPNILVNSSRGIIFASQDHNFASAAREEARKIAESEYL"
     gene            complement(5482..6018)
                     /gene="pyrR"
                     /locus_tag="PLB65_06080"
     CDS             complement(5482..6018)
                     /gene="pyrR"
                     /locus_tag="PLB65_06080"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOQ78133.1"
                     /translation="MQVKSQIMDKDQMERSLQRMAHEIIEQNRGLEKIRLVGIRSRGV
                     PLAERLSSYLKLITNQEIPVGILDITLYRDDLSTISHQPIIKGSAIDFDIEDSIIVLV
                     DDVLYTGRTVRAAIDALMDFGRPKQIQLAVLIDRGHRELPIKADYVGKNVPTSKEEII
                     KVALEEIDGEDSVQIVLV"
     gene            complement(6031..6492)
                     /locus_tag="PLB65_06085"
     CDS             complement(6031..6492)
                     /locus_tag="PLB65_06085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOQ78134.1"
                     /translation="MYNYKDMQVWQVSMQLCVDIHKRTQEFPQFEQYELAAHLRKTAL
                     SIASNISEGAGRKSTMDFIRFLNIANGSLSEFETQLEAAFMLGYLPEFNEYLKRIAHL
                     SSLISGLVRSLNQKIRSMDKNETLNSYAKNCPILYYVQPGFHPFTLSPIHL"
     gene            complement(6604..8589)
                     /locus_tag="PLB65_06090"
     CDS             complement(6604..8589)
                     /locus_tag="PLB65_06090"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase subunit B"
                     /protein_id="HOQ78135.1"
                     /translation="MDKHSCVSKTTMHYEPDRPKAANPVKVQDLTFRDGHQSLFATRG
                     RTEDMLNVAELMDQVGFYSMEVWGGATFDTMHRYLGEDPWDRIRILKKHITKTPFSML
                     LRGQNLVGYQNYADDVVEAFVQRSCDNGIDIFRVFDALNDFRNFQTAVKIIKKNKKLF
                     EGAICFSLTEQRMGGDIYNIDYYVKKAKQLEEMGADTICIKDMAGIVAPYDAYDLIKA
                     LKENVKVPVHLHTHFTSGMGDLALLKAIEAGVDIIDTCVGPYAYRTSHPAIEPLVITL
                     LGTNRDTGFDIKLLNKIGKEMEKDIPKYMEFADTTKFSIIDTDVIIHQTPGGMLSNLV
                     NQLREMDALDKLEDVFKEIPKVRKDLGQIPLVTPTSQIVGIQAVNNALFDTYEGEYAR
                     ITEQVKDLCYGLYGKTTLPINPEVQKKALKGYPKGETPITCRPGDVIPPAMEKVHKEC
                     EGLAKNIDDELIVALYNITGKRFLRIKYGLEPMPEEMKPKTLEDIKKEEELINKAKAG
                     KLIEKTEPKEKPQDLRQFDVYVDDTYYLVEISEKGGAPKIISSRPAIHNSDPVAKPVN
                     NPAPQVRASNPAPEPKSAPVTSIEGGTKITSPMPGMFVRYEKKIGDPIKQGETVLVLE
                     AMKMYNNIPSPVDGVLVDAPFSGGANVAKGDVLAVVK"
     gene            complement(8602..8790)
                     /locus_tag="PLB65_06095"
     CDS             complement(8602..8790)
                     /locus_tag="PLB65_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78136.1"
                     /translation="MKDKKEIAAIGAVLALLQSEETYEQYKEPVRGIPSQPYTAYGRQ
                     LTMQYRDLTQRRVIKRSR"
     gene            complement(8812..10365)
                     /locus_tag="PLB65_06100"
     CDS             complement(8812..10365)
                     /locus_tag="PLB65_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOQ78137.1"
                     /translation="MQSTKDAIQGLLEKEKKILQMGGDKAVAKQKQGGKLTARERIDL
                     LFDEGTFRELDMFVSHRCDNFGMEKIEIPSDGVITGHGLVDGRPVFAFSQDFTSRGGS
                     LGEMHAAKICKVMDMALKSGVPLVGIQDSGGARIQEGVDALKGYGDIFFRNSRASGVI
                     PQITAIMGPCAGGAVYSPAMTDFVFMVKNTSFMFITGPDVIRTVTGEDTNQEDLGGAM
                     AHNSKSGNAHFACESDADAIEQIKVLLSYLPSNNMEEPPRIKTNDDPGRLCPELDSII
                     PDDPKMSYDMRDVIHSVVDDGIFFEPHYYYAQNVIIGFSHLNGRTIGIIASQPAFLAG
                     CLDIDASDKATRFVRFCDAFNIPLITFVDVPGYLPGTHQEWNGIIRHGAKLLWCYSEA
                     TVPKLTVVTRKDYGGSYIAMSSRHLGADMVFAWPTAEIAVMGAQGAANIIFRKEIASA
                     EDQEAKRQEMIKIYEEQFNNPYVAASRGYVDAVIVPSETRKRLIDALEILCTKSESLP
                     PKKHGNIPL"
     gene            10815..>12713
                     /locus_tag="PLB65_06105"
     CDS             10815..>12713
                     /locus_tag="PLB65_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HOQ78138.1"
                     /translation="MARLFAKDYHNKSTSWYFNQAVIVLEKDDLWFWKTRNEEGNYQI
                     RFFPVPQKREYFTEYVDIVFNPETELIISHNCSQCNTDESCRHYLSVLRYAYLYLSTR
                     IFEEDTVETCDGNALAGDELWLNLARNAKLYVEGIYNPETDKIRFYHNELSPLDPVLI
                     GKLYAQEEITDISERQVEYYLSNLSAFWDEELHLFSFLNKNRAAYSRKSKFWSIYKKD
                     FAAALLIMQKCRTRFIVKETGEELIFQEQTYPLALRIEPAGTNNYSLRAVVVDELSVW
                     FTGNPSWLFFRNRIYKIYLPFRKEVVDNIFSSGEILSTRDLVYYRCIVHRELHEHNIY
                     LDFEDSIVLPEIVDETPLKRLHIKKLGDKVLVGGSLAFSGEREIPLSVLRFQKPLIHS
                     KYVSPAGEGNAWFHLSPELYEKTQELLEKLPEPELNRLEEYAELVFSGEERIEQLRTA
                     IFNLSDEDWDIQIDPELQNYFISKIPLQVEIIARTDEDIDWFTYEVRYRYRDLNFTHE
                     ELKRFFRSKEEFLHTAEGRIVFISNPQVFYEVEELISHSEQKADKVYRSRLMNLPYYL
                     RLREENPAFRMLGDEWLEGLYDALLNRHLKTFESLPLYLQTVLRGYQKAGVAWIKMLA
                     HYHLNGILA"
BASE COUNT         3912 a         2487 c         2243 g         4071 t
ORIGIN      
        1 aacttttgca gggcaacatt cttccaattt tacaatcact ttgttaataa aatctccata
       61 tttctgtctt ctatcttagt tgcttgagga tgttaatata gcgttcatca tcccttaatc
      121 aagcgttaac aattaacgct tgattaaggt tagattaaca cttgattgac gcttggaaga
      181 aagagagaaa agcgaagctg gcatcgatat gctgtctatt aatgtagcaa ataccttagg
      241 gtagataaat atattaggat ataacataat agcgacccaa agttttacac tttaggaaaa
      301 atgggctaac caatcctctc ttagcaacta aactgaattt cactaatgca cttaacacag
      361 ctaatacagt tacaaattgg tagaaaactg gatcacttac aaaattatga ttacagctag
      421 tattcagaag tttgcatagg ataagtttct actgcagtgc aggttaagaa tcataacata
      481 gctataggat ataatgttaa ttataaagca ctctatatta ttataaactt accgtctggg
      541 atgaaaatta aagaaatttc aactgtggaa aaaagtactt gccaagaaaa tagtgcaaat
      601 aattgttacc ttcattggtg atcgcgctta catttgtcta taattgtgct taggagaatt
      661 tatatgagat ttgatgtatc ctatttctgt cattacggca atagagcatt aaagaatcag
      721 gatagagtta ttgtgaatgg ggatattgta tcctgtggag taaagcatca ggaaaacatt
      781 gctgctctat tttgttttgt agcagatgga attggttctt taattaatag tgagaatgct
      841 gctcaatatg ttttggagca gattgatttt ctgcgtttga ggcctggtct tgattggtgt
      901 aatttaattg actccttttt acaaaatact aatctggaac tggtgaaaat gaatcacgag
      961 agggatgact ttgaagattc agccacaact ctttgcgggc ttatttacag ggacaagatt
     1021 tttattccca taaatgtggg tgattcggaa atttggctat atcggaacaa agaactactg
     1081 cgcattacaa aacctcatat agaagatgaa tgcattccta atagccctat aaacaattat
     1141 ttcggttcct tccaagctca tatgtatatt gattttatta cttctattcc ttgtttatta
     1201 ccggatgata aactggtggt tactacagac ggtcttttga aagctattag caaggaagaa
     1261 cttattcata tattggaaga taacgcaccg ctgaagaaca aaataaatgc tcttttttat
     1321 cagctatcag ctaaagataa tcctgataat ctgggagcaa tcttcattca ggtaaaaaag
     1381 gacaaataac ccattattta actactgcta gtttattgct tgccaaaggt tttcccgctt
     1441 tgtcccgtaa aaccaataga taaatccctg ccgctacttt tgtggaatta aaactattta
     1501 aatcccagtt tagcgtgctc tcttttgtaa ttctcgatat ttctatactt ctcaccaatt
     1561 gacctttcag gttgtaaata ttcagttcgg caggttcagg gctattttta taaatggcaa
     1621 tattagcata agccgttgct ggattaggat aagcagatat ttttgctatg ggtattggcg
     1681 gaattacggg atcttctatt cctaccaaaa aactggttaa ataaaagcac ccatcggaat
     1741 ttaacacata aaaggaaaag gtgtcgatat agggaaaaat atccatctgc ttaactggtt
     1801 tattgataga aggatgatta aagtgagata cggttccatt ggagcgaaac attcccacat
     1861 aggaactatc ctcgtaacat tctctccagc caagagttac attgttattg aaaacaggtc
     1921 ctatgcaagc aagggcggaa gtataaagaa tatttaccca gtttgcgcca aaatcattgg
     1981 aatgccaaag tccgtccgaa tcacttgtta catccataat ggcatataaa atgccattgt
     2041 tgtcaaacct gaaactatgc agattgctaa ttgatgattg atgccagtta agtccattat
     2101 cattagtata gtaaactatt tggccattat tcgttataat attgttacca taccaggcaa
     2161 gggaactaca ctcatttaaa ccgagagaat taatccgtga ccagttatgt ccattcgttg
     2221 aacgatacaa accatcacgc tcgcccacat aatacaattg actattggga ttatacttta
     2281 cgaaattagg aagtagaaac caatcattca attgccaggt atgcgtgtgt atatcaaagt
     2341 tataaattcc atcactgtat gtcattgccc ccatagcaac cagcattgtg tctgcattca
     2401 gcatacaagt acttataaca ggtaaaccat taacataata ggaatagtta ttccattcac
     2461 cattttccca aagacaaagt ttttcttgct catttatccg cgttccaaaa ctggcgggct
     2521 cctggtttaa attggcacaa ttaaagtcat ctgcctcagg accaaataca tcaacctgga
     2581 aagcagaaca tataaccacc caactaaata gcataagaat aaaaatcttc ctcatatctt
     2641 cctcctcttc cagaaaaatg agttcagaat atcaattcta tatataatgt aaatgaaaag
     2701 aaagaaaaag caaattaacc acttgcacaa gattaattta cttttgtggt gatatcgggc
     2761 ctccagtaat cacagaaatg ctggtgaaca atgtacccaa ttgactagga gatagatttt
     2821 gcaagtactc taaattgaga aatgattttg caccgggcat aatttccttt acaatggatt
     2881 tttggctctg ctccaaaatc caatttatta tataatcctg ttcagcaaaa ctttgctgat
     2941 gattgattgc ttctttatgg cttctatatt ttaagcgaat tttacgagtt tggttaacca
     3001 atatgcggta aaaaatattt cgcattttgg caggagacca ttgataacgc gcatatctta
     3061 attttgccag ggaaggcgat aatgcgtata gaaaattgta atatagcaca ttatctttct
     3121 tctgctccat tggtatctgc attgctaatt ggtaaaacct aaaatcgaaa aaaggtgttt
     3181 ctgaacggaa gaaataacga ttacgatctt caccttcaaa agcccaacga gcagaacgct
     3241 ccgctaaaat ggcagaagca tatttatagt tataatttcc tgtcggataa gtagatagat
     3301 ggttcaaaag atattcatcc atctctttct ccggaatacc aaaaattgct gctgaatctt
     3361 tagtagggat gatggcattc tgactatacc aataattcag ccactgtttt tcatcggcta
     3421 actctttatc aggtaaaaga taacgcataa ctttgtcacc cccgtctcca gttaaaaaca
     3481 aagcagattg aggatattga gccaaaatct tctccaaata ttgcagaaac atagctactg
     3541 gaagatagtt caaacctgcc tttaaataaa acaacttttc ataatgctcc ggttcacaaa
     3601 gagaaagctg tataacatta tgagatttat ggtataactc ggctatctga gaagcaataa
     3661 aaacatcatc ttttacctct ttctgaaaat ccagaaaaga tatggggaca aaatctatac
     3721 ttttttgttg aagagctcca gccacacaac gcgaatccat ccctccgctt aaagacaaaa
     3781 gtttgggaag ttttgtccga tacgaaacac atacatccat aaatgtttct acaagttcat
     3841 ttagcctttt tttttctgct ctaccggcaa ggggctcaac aaatctcata tttggttggg
     3901 aaatcatttg taaatcgtca gttaaccaat cataaatggc aaaagttcca cttgccagag
     3961 tatctatttc ctgataaaag gttccgcgtc cgggaatata ggcatttgaa agatataaag
     4021 ccataaataa aggatttaag gttaactgat cgctgattgc ttttacccag gaaatatccc
     4081 tgcccaaaat aaaacaatcc ttttgttggg tgtaataaat gggaagacgc gccaaagcat
     4141 cattggcaaa gattatttta tgcaaatctg gattcacggc aagcaaaaag aaactttcgt
     4201 aagtattgtg gagaagcata ttccagctct ttcttagatt tgctaaagat atagaattat
     4261 tttctacctg ctctaaaata tcggtaacag tttgcgttat aagaatattg tcctgtagtt
     4321 cgtgaaatat ttgccagccc ttataactct ctgtataaac ctgatagccc tgatgctgag
     4381 aaaaagttaa caacacttca ctacaaggca taaaattcgc taattgcaaa tgaggaaaag
     4441 tagctccaaa cctttgcaga tggagatcta tagtatttcg gttctgtgga gtaatcccct
     4501 tggcattaat caggcaattt attcctggca ttggttctcc ttaatttcca gaagattcaa
     4561 ctttggtttt atccgtttta aaaaacgatt cggaatccaa ttgggataat gctgttgaaa
     4621 tcaatatcta ctcgaatatc ttaagataga gagaggaaat cccttaagca attattttaa
     4681 ccaaggaaca gggagattca ttctctctgt gatacttata aatattcgct ttccgctatt
     4741 tttcttgctt cctctctggc agcagaggca aaattatgat cctgtgaggc aaaaatgatt
     4801 ccccgcgagc tattaactaa gatattaggt gttgcttcac tatatgttgc ctgctgtaaa
     4861 acagttttta ggtctccccc ttgagctcca attccaggaa taagtaatat ccgatcctgc
     4921 aataaattac gcatcttttt caagtctgca ctttgcgttg ctcccaccac agctccaaaa
     4981 ttttctttgg gataattttg cagccagtta gctatttttt ctgccatacc atctttcaaa
     5041 aaatcggatg ctcccggatt ggaagttaag cataaaacaa aggcaaaacc gttcttctct
     5101 agaactggtt gcattacatc ttttcccata agtggattaa gagttatagc atcaacttcg
     5161 aggtccttaa aaaatgccgt tagataggca ttcattgtgt tgccaatatc accaactttt
     5221 acatccaata tgactggaat attatccgga ataaaatcta ctgttttgta taaagcatcc
     5281 aaaccgcgta atccgtctgc cagatagaaa gctaaatttg gtttataagc acaagcataa
     5341 tccaaagttg cttcaattat ggctttattg aattcccaaa ttgggttttg ggcatttttc
     5401 acacatttag gaagctggtt aaaatcggaa tccaggccta cacaaagaac cgattttgta
     5461 ttattatagc gttcctgata tttataccaa aacgatttgg acactatctt ccccatcgat
     5521 ttcttctaaa gctactttaa ttatttcttc tttggaggta gggacatttt tacctacata
     5581 atccgcttta ataggcaatt cgcgatgacc gcgatcaatt aatacagcca attgaatttg
     5641 tttcggtcta ccaaaatcca tcaaagcatc aatggcagct cgaacagttc ttcctgtata
     5701 aagtacatca tccaccaaca caattattga atcctcaata tcaaaatcga tggcagaacc
     5761 ttttattatg ggttgatgac taattgttga aagatcatca cggtaaagag taatatccaa
     5821 aataccaacc ggtatttcct gattagtaat aagttttaag taactggaaa gtctttcagc
     5881 caaaggaact cctctggaac gaatacccac caagcgaatt ttttccagtc cgcggttctg
     5941 ttcaataatt tcgtgagcca ttctttgtag ggaacgctcc atttggtctt tgtccattat
     6001 ttgacttttt acttgcattt tacctcctaa ctacaagtgt atgggtgaaa gggtgaaagg
     6061 gtggaatcca ggttggacat agtaaaggat gggacaattt ttagcatagg agttgagggt
     6121 ctcattttta tccattgacc taattttttg gtttaaagaa cgaactaaac cagatattaa
     6181 actgcttaaa tgggctatcc ttttaagata ttcattaaat tcaggtaagt agccaagcat
     6241 aaaggcagcc tccagttgag tttcaaattc ggataaggaa ccattggcta tatttaagaa
     6301 tcggataaaa tccatagtgg actttctgcc tgctccttca gatatgttgg aagctatgga
     6361 tagggctgtt tttctaagat gagcagccag ttcatattgc tcaaactggg gaaattcttg
     6421 tgttcgctta tgaatatcta cacaaagttg catactcact tgccaaacct gcatatcttt
     6481 gtaattatac atttcatctg cctttaccta tatttgtttt aagaggcatt tatcccctgt
     6541 caacatataa caggactgca acacaattta tccctttgtt tcactttctc acctttccac
     6601 tttttacttc acaacagcta acacatcccc tttggcaaca ttcgctcctc cagaaaatgg
     6661 tgcatcaacc agcactccat caaccggaga gggaatatta ttatacattt tcattgcttc
     6721 caaaactaaa actgtttctc cctgtttaat gggatcgcca atcttttttt catagcgcac
     6781 aaacatccct ggcattggtg aagttatttt tgtgccacct tctatagatg ttacaggagc
     6841 tgatttaggt tccggagcag gatttgaagc tcttacttgg ggagcaggat tattaactgg
     6901 tttagcaacc gggtctgaat tatgaattgc aggacgcgaa ctaataattt taggagctcc
     6961 ccccttttca gatatttcta ctaagtaata agtatcatcc acataaacat caaattgacg
     7021 cagatcttgc ggtttttctt tgggttcggt tttttctatc agttttccag ctttagcttt
     7081 attaatcaat tcttcttcct tttttatatc ttccagagtt ttgggtttca tttcttccgg
     7141 cattggctcc aagccatatt ttattcgtaa aaatctcttg ccggtgatat tatagagggc
     7201 aacaatcagt tcatcgtcta tgttcttagc caagccttca cattctttat gaactttttc
     7261 cattgcgggg ggaattacat ctcccggacg gcaagtaatg ggagtttcac ctttgggata
     7321 acctttcaat gctttctttt gcacttccgg attaatcggc aaagtagttt ttccatataa
     7381 accatagcaa aggtctttca cttgttccgt tatgcgtgca tattcacctt cataagtgtc
     7441 aaacagagca ttgttaactg cctggatacc aacaatttgg ctggtgggag taactaaagg
     7501 aatctggcct aggtcttttc gcactttagg aatttcttta aacacatctt ccaatttatc
     7561 taaagcatcc atttctctta gctgatttac taaattggaa agcattcctc ccggagtttg
     7621 atgaataatc acatcggtat cgataataga gaacttggta gtatctgcaa attccatata
     7681 cttgggaata tctttctcca tttctttacc gattttgttc agcaacttaa tatcaaaacc
     7741 ggtatcgcga ttcgttccca aaagggttat caccaatggc tcaatggctg gatgcgaagt
     7801 tcgataagca tagggtccta cacaagtatc aataatatcc acccctgctt caattgcctt
     7861 tagcaaagcc aaatctccca ttccggatgt aaagtgagta tgcaaatgca ctggaacttt
     7921 cacattttct ttcagtgctt ttataagatc ataagcatcg taaggtgcaa ctatgccagc
     7981 catatcttta atgcaaattg tatcagcacc catttcttct agctgcttag cttttttaac
     8041 ataataatct atgttataaa tatctccgcc catccgctgt tcggttaagg aaaagcaaat
     8101 ggcgccttca aagagctttt tattcttttt aataatttta acggctgtct ggaaattgcg
     8161 aaaatcgttt aaagcatcaa acacccggaa aatatctatg ccattatcac aactgcgctg
     8221 aacaaatgct tccactacat catctgcata attttggtat ccaaccagat tttgacctct
     8281 aagcaacata gaaaagggag ttttggttat atgctttttc agaatcctaa tgcgatccca
     8341 aggatcttct cccaaataac gatgcatcgt atcaaaggtt gccccacccc agacttccat
     8401 agagtaaaaa ccaacctgat ccatcaattc tgctacattt agcatatctt cagttctgcc
     8461 ccgagtagcg aaaagtgact gatgtccatc cctgaaagtt aaatcctgaa ctttaacagg
     8521 atttgcagct ttcgggcggt ctggttcata atgcatagta gttttactta cgcaactatg
     8581 tttatccatt tatatctcct tttatctgct tcttttaatt actcttcgtt gagttaaatc
     8641 acggtattgc atagttaact gtctgccata ggcagtataa ggctggctgg gaatacccct
     8701 taccggttcc ttgtattgtt catatgtctc ttcactttga agaagagcta aaacagctcc
     8761 aatggcagca atttcttttt tatctttcat aatcttgttt ccttgttttg gttagagagg
     8821 aatgttgcca tgttttttag gaggcaaact ttcactctta gtgcaaagta tttccaaagc
     8881 atctattaag cgtttccgag tttctgaagg tacgataaca gcatccacat aaccacgcga
     8941 agcagcaaca taaggattat tgaactgctc ttcataaatc tttatcattt cctgcctttt
     9001 ggcttcttga tcttcagcag aagcgatttc tttccggaaa ataatatttg ctgcaccttg
     9061 agcacccata acagcaattt cagcagtggg ccaagcaaaa accatatctg cccctaaatg
     9121 tctgctactc atagctatat agcttccccc atagtccttt ctggtaacca ctgtcaattt
     9181 gggaaccgta gcttcggaat aacaccataa aagtttggct ccgtgacgaa taatgccatt
     9241 ccattcttga tgcgttcccg gaaggtaacc tggcacatcc acaaaagtga ttagcggaat
     9301 attgaaagca tcacaaaaac gcacaaaacg cgttgcttta tcggaagcat caatatccaa
     9361 acaaccggct aagaaagctg gctgactggc tatgatgcca attgtccttc catttaaatg
     9421 actaaaacca ataatcacat tttgggcata ataatagtgg ggctcaaaaa agattccatc
     9481 atcaaccacg ctatgaatca catcccgcat atcataactc attttaggat catcaggaat
     9541 aatggaatcc agctctggac acaaacgccc gggatcatca tttgttttta tccggggtgg
     9601 ttcttccata ttattagaag gcaaatagga cagcagcact tttatctgtt caatggcatc
     9661 ggcatcactt tcacatgcaa aatgggcatt tccacttttg ctattgtgag ccattgcacc
     9721 ccccaaatct tcctgattgg tatcttcgcc agttacggta cgaattacat caggaccggt
     9781 aataaacata aagctggtat tcttcaccat aaacacaaag tccgtcattg caggagaata
     9841 aacagctcca cctgcacagg gtcccataat tgctgttatt tgaggaatta caccactggc
     9901 acgcgaatta cggaaaaaga tatctccata accttttaga gcatcaactc cttcttgaat
     9961 acgggctcca cctgaatctt gaataccaac taaggggact ccacttttta aagccatatc
    10021 cataacttta caaattttgg cagcatgcat ttctcccaag gaacccccac gagaagtaaa
    10081 atcctgactg aaggcaaaaa ccggacgacc atctactaaa ccatgaccag ttataactcc
    10141 gtcggaaggg atttctattt tttccattcc gaaattatcg caacggtgcg agacgaacat
    10201 atccagttcc cgaaaagtgc cttcatcaaa taggaggtct atacgttcac gggcagttag
    10261 ttttccccct tgcttttgtt ttgcaacagc tttatcacca cccatttgga gtattttctt
    10321 ctctttttcc agtaagccct ggatcgcatc ttttgtagat tgcattagat ttcctcactg
    10381 tgtatataat aattaatcga aaataaaaaa caaattgctt ttactaccct tgttaatagg
    10441 gatagagtat aaatttacat tgtgttctcc ttctagtgca ttattttttt agtggataca
    10501 gtgctttgtt gatggcaact ataatcctat agtatttata gctattttgc ctttttctca
    10561 tagagctttt ttttgtcaaa tcaaatttat gtaaaacctg taagttgata tctatgcata
    10621 ctctacccaa atatctataa agtaatctaa aatgaatggg aagcttcata acttggctgc
    10681 aaaaagagca atgaggacat cgatcttccg acattatagc agcagaatat ggtttttatc
    10741 gtttttttca tttgacagaa ttgactatat tatcctatat gaattaatta atattaaaag
    10801 gactgaataa atcgatggct cgtctttttg caaaggacta tcacaacaaa tctaccagct
    10861 ggtatttcaa tcaggcagtt attgttctgg aaaaagatga tttatggttt tggaaaaccc
    10921 gtaatgaaga gggaaactat caaattcgct tttttccggt tccccagaaa agagaatatt
    10981 ttactgaata tgtagatatt gttttcaatc cggaaaccga gctgataatt tctcataatt
    11041 gttctcagtg taatactgat gaatcctgtc gtcattatct atctgtttta cgctatgctt
    11101 atctttattt aagcacgcgt atttttgaag aagacacagt tgagacctgt gatggtaatg
    11161 ccttagcagg agatgaatta tggctaaatc ttgctcgcaa tgcaaaactc tatgtggaag
    11221 gaatttacaa tcccgaaacg gataaaatcc ggttttacca taatgaactt agtcccttgg
    11281 acccagtttt aattggcaaa ttatatgctc aagaagagat aacagatatt tctgaaaggc
    11341 aagttgaata ttatctttcc aatttaagtg ccttttggga tgaagaactg catcttttta
    11401 gctttttaaa caaaaaccga gcggcctata gtcgtaaaag caaattttgg tctatatata
    11461 aaaaagattt tgctgcagca cttttaataa tgcaaaaatg tcgcactcgt tttatcgtta
    11521 aagaaaccgg agaggagctt atttttcagg aacagaccta tccgcttgct ttaaggattg
    11581 aacctgctgg aactaataac tattctttga gagcagtagt tgtagatgaa ctttcagtat
    11641 ggtttaccgg aaatcctagc tggctatttt ttcgtaatcg gatttataaa atttatctac
    11701 cttttcgcaa agaagtggtc gataatattt ttagcagcgg agaaatactt agtactcggg
    11761 atttagttta ttatcgttgt attgttcatc gcgaacttca cgagcataat atataccttg
    11821 attttgagga tagtatagtt cttccagaaa ttgttgatga gacacctcta aagcgattac
    11881 atatcaaaaa attgggcgat aaagttctgg ttggaggttc tttagctttt tccggcgaac
    11941 gagaaatccc tctttccgtg ttgcgttttc aaaaacctct aattcattcc aaatatgttt
    12001 ctcctgcagg tgaaggcaat gcttggtttc atctttcacc tgaactttat gaaaaaacac
    12061 aggaattgct tgaaaaactt cctgaaccgg aattaaaccg tctggaagaa tatgctgaac
    12121 tcgtttttag tggtgaagaa agaattgaac agctacgaac tgctattttt aatcttagtg
    12181 acgaagattg ggatattcaa attgatcctg aactgcaaaa ttacttcatt tctaaaatcc
    12241 ctctgcaggt ggaaattatt gcccgtacag atgaagatat tgattggttt acttatgaag
    12301 tgcgttatcg ctatcgagat ttaaatttca cgcacgaaga gttgaaacga tttttccgca
    12361 gtaaagagga atttttgcat actgcagaag gcagaattgt gtttatcagc aatccccagg
    12421 ttttttatga agtggaagaa ctcatttctc acagtgaaca gaaagcggat aaagtatatc
    12481 gttctagatt gatgaatctg ccatattatt taagattgcg cgaagaaaat cccgctttcc
    12541 gaatgttggg agatgaatgg ctggaaggac tttatgatgc cttactaaat agacatttaa
    12601 aaacttttga atccttaccc ttatatttac agacagtatt gcgtggttat caaaaagcag
    12661 gtgttgcctg gattaagatg ttggcacatt atcacttaaa tggtattctt gcc
//
LOCUS       DAOSTH010000047        11831 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_5393_length_11831_cov_15.313434, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000047 DAOSTH010000000
VERSION     DAOSTH010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11831
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5393_length_11831_cov_15.313434"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            170..1933
                     /locus_tag="PLB65_06110"
     CDS             170..1933
                     /locus_tag="PLB65_06110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HOQ78139.1"
                     /translation="MNEVLLTLESVSFLYGDRIILQDVSFGIHNSEKIGIVGINGSGK
                     TTLLNIIGGIIKPSTGIVTSRKDLKIGFLEQNPQFIPELSVLQHTIPTQGEIKDEYHY
                     KSILSRLGIEDYNQKMILLSGGQRRKADLARVLALEPDLLILDEPTNHLDLDTIEWLQ
                     NYLADTNKAIIFVTHDRYFLDAVSTKIIEIERTRLFYYEGNYSAYIHGKLIRATDSQR
                     KETRRQAQLKKELDWLNRGAKARTSKPKNHIDRVKELLSKSYLISNQDLDISFQIDRM
                     GKTILELHRLSKSYGSRVLFEDLDHNFQNRERIGIIGPNGCGKTTLLKIIAGEDEPDI
                     GVVKAGINTHFAYYKQDEDLFDPNTTVYEYIAQFAEVIKTENGNKITAAEMLKRFLFD
                     GKMQQMKLGSLSGGERKRLYLLKALMFGANFIIMDEPTNDLDIRTLEILEDYLDAFKG
                     CLLIVSHDRFFLDRTVDYLFIFQEGKIRKFAGNYSDYLLVKRFEQEEDTTKKKPAILR
                     PKRISKGLTYNEQREFSQTEKDIEKIENQLIELEDKLNDFSQTLTPNDYHQISITILK
                     LEEQHQNLLNRWLELSDKQDN"
     gene            1980..2552
                     /locus_tag="PLB65_06115"
     CDS             1980..2552
                     /locus_tag="PLB65_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOQ78140.1"
                     /translation="MISELVYRNRSYRRFYESKRITHSQLIQIMETARFIPSAGNLQK
                     LCYYLIEERSECDKMFPHLKWASYLKKWNGPEPGERPSAYIIILVPKSCSNMVYIDTG
                     IAAQTILLTAVDNGLGGCLLASVDKDAVHKIFLLPDNMDIVLVIALGYPAETVVIEDV
                     TDQDKIEYWRDENTVHHVPKRKLSDLILNY"
     gene            2622..3962
                     /gene="xseA"
                     /locus_tag="PLB65_06120"
     CDS             2622..3962
                     /gene="xseA"
                     /locus_tag="PLB65_06120"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOQ78141.1"
                     /translation="MENVIVYSVYEVTRQIRQVIESSIEPLYVMGEISGFVHHSSGHI
                     YFNLKDENSSLRCTFFKGANYNLTFKPEDGMQVICYGKLTVYEKGGTYNLNVLKMQEA
                     GQGLLQQKFEILKKKLEKEGLFATAHKKPIPKYPERIGIVTSPTGAALQDILNIISRR
                     YPVEIIVYPALVQGLEAPPQIINGIKYFNTTKDVDFIILTRGGGSQEDLFCFNDESLV
                     RAIFDSDLPIISAVGHEIDFTLADFVADLRAPTPSAAAELAVPDKKDLLNLLNSLKKR
                     LDLTVQNKLEMKHKPIMNYQLQLTQYHPEKVWREYQQRFDLAEMAMKNALKQLPDKRL
                     QFEMRTQTIMQQVTSKIDLCIAQAKANSNLAWHQLNDKTLNLLHNKANHLNSLGNILE
                     QLSPRSIQRKGWIMAFKDGKLISSVKQITKGNHLNLDFIDGIADSIIERVENKE"
     gene            3974..5407
                     /locus_tag="PLB65_06125"
     CDS             3974..5407
                     /locus_tag="PLB65_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HOQ78142.1"
                     /translation="MIFSLLFLLKLSAEIRSVWVLPWDITTESAIDQVINTAVDCNQN
                     ELLVEVRYRADALFDTSQGAYLYPNPEPVSYILNNSSFDPLTYIIQQAHNNGLAVQAW
                     IVVFNATQTDHYLIRQNYIYNNHRNWITYDKNGYQMNNGYRYGYFIDPGIPEVQEYLL
                     NIVGNIAAGYPELDGIHLDYIRYPDADLGYHPISQELYRAYCLNQMDITYNEWRIMQI
                     NNFVEKAYHLIKEINPSLLLTAAVFADIADANVSYAQDWQSWLKNGIIDRVYPMAYDI
                     NYAKFSKHTEQMKLLNFDSKIVIGLRAWNENGSSLALGKGASYNVQNVARKIDLAREQ
                     GFAGISLFSYSGLIKGNAWAQLTRLSYSEKAEYPFEKVSTPLVNQSVTELKQPPDVKL
                     LRLNNEILIQLQIPSEGLWNWELYKESFLLQHNRYYLKGNNIDILNVINPNICASIEP
                     GQYIVQLYQENSSFKYFIPVELKIANE"
     gene            5400..6863
                     /locus_tag="PLB65_06130"
     CDS             5400..6863
                     /locus_tag="PLB65_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HOQ78143.1"
                     /translation="MSDLPKQAGILSATEFFRLAIKSIIGIALARIITPAELGSYRQL
                     FLIYTTFSTLLLLGIPQSMLYFLPKAENAEKQRLLITRSLNLISILALIFALCLLIFR
                     NFIAQIFHNPQLGNLLIIYALYPLFLFVTQLYSSIMMGLKQPLRAAKFTIFSVLSDFI
                     FIVSAAFFTRNLTIIIWAVIASAFLQWLYAQITLNEFKEKKVRFDFASVKEQMNYALP
                     LGLSSIIGMLSIQLDKLMISGFFPPEKFAVFSLGATEFPIVGILANSINSVLLPHLSS
                     TNTATMGELYSGAVRKNAILVFPLMALCFIFAEPIFILLYGKIYAEAAVYFRIYLFLM
                     PLRIATYGILFQAFGKTKFIMYNSLFVLIANAVLNYILIINLGMKGAAIATVIVTWLS
                     VMIYLIQIAKVLKLKLLDFFPIGKITKNALVTIISLFLSFLIIWLGKQNLLFVAIGGI
                     VFILSYLLLGRKTGVILDYDVQLAKELINDLKGKFKK"
     gene            6860..7309
                     /locus_tag="PLB65_06135"
     CDS             6860..7309
                     /locus_tag="PLB65_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78144.1"
                     /translation="MNYYAESTWKNYDPEQQADIRIAKLLSLFPENMHYYKYTNCGLT
                     YNAYGHLQSFSKNTLNSLLTPEFSFTAQDILCPLNRDFIPFLLDIKQHWLKQWFNPPS
                     GTIDSNCGKSEFLNKKSLFTKSINYFINMSTKPQPYWFMELFVWREN"
     gene            7312..8583
                     /locus_tag="PLB65_06140"
     CDS             7312..8583
                     /locus_tag="PLB65_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HOQ78145.1"
                     /translation="MKNLLFITYHFPPTGGSGVQRGLKFAKYLPQFGFRPIVLCADYR
                     CLKQPKDYTLLKELPPSVKIYRTFTLDANWFFKLLWGLKLNKVVTFLQRYILIPDTEV
                     LWLPFAFLKLRKILDANHIDLVLISAPPYSSLFLAKYCKHRYQLKVCVDFRDPWSFGI
                     GRKYLKPPSWIANKENNWEKEIVTLADKIICVNDKMVDEFRQLHTKIPAVKFCSITNG
                     YDEEDFSISTPLKKSDKFKIVYTGSFYDARRPLILWQALWELIEEGKINPQKITVNIY
                     GRNTKNFVLGEYQDNLLIQKIVHFYGYTPHTQIIKILLSADVLLLYSGFGNTEQLNSP
                     AKLFEYLRSGKPVFAIIDPESSAAEILLPANTIFLANSASLSSIKNTLSELYTQWEEG
                     KLKVSPDWEYIKNFERKALTARLAEVLSSIT"
     gene            8583..9752
                     /locus_tag="PLB65_06145"
     CDS             8583..9752
                     /locus_tag="PLB65_06145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOQ78146.1"
                     /translation="MKILIIPSWYPYPKKPYAGKFFIEQAKALSENSNWQFEILNWGQ
                     NEFQMQIRYPLSSIAKLFAYLKAKPSSRNFNSNLKELFIPHITWTSKLLSGNIKTLIN
                     KMELIPKPDIIHSHVTFPAGFVAMLFAEKWKIPYIITEHSGPFPFPEFVSNNRISKKI
                     TLPLQKAAAVISVSRYLQKQILSTCLVKSIVIPNLIDTEFYKPVSISEKRRRFSFFSL
                     SALIPAKGVLDLIEAFRLAVNKGMDADLYLGGSGYLAPKLRKLIIQYHLENRIHLLGY
                     LTPQQALEQFNKCNCYILPSHIESFSMVLLEAMACGKPIIATDCGGPKDIITETTGVL
                     IPPQKHDVMAIEMLKMANNTNKYNPDSIREWCLKNYSPHIICAQISRVYESITGS"
     gene            complement(9761..10129)
                     /locus_tag="PLB65_06150"
     CDS             complement(9761..10129)
                     /locus_tag="PLB65_06150"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ78147.1"
                     /translation="MTKILVVEDDVSFRNVLVQMLKNSGYFVQQAGDGDQALEICCEF
                     DPDIVITDIIMPNKEGLETIQDLMAIKPGIKIIAMSGGGKYGPYSYLPLALKLGAKAV
                     LEKPFMRNEMIEVIEKVLNS"
     gene            complement(10313..10388)
                     /locus_tag="PLB65_06155"
     tRNA            complement(10313..10388)
                     /locus_tag="PLB65_06155"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10353..10355),aa:Phe,seq:gaa)
BASE COUNT         3936 a         2066 c         2099 g         3730 t
ORIGIN      
        1 cctgtagctc agctggatag agtggcaccc tccgaaggtg aaggtcaggc gttcaaatcg
       61 cctcagggac gccacaaaaa tgaacaagtt aaccaataaa tgggattaac aggttaacag
      121 atgaaaaagt gatatcctac atctttatta tcggtattat ataaattata tgaatgaagt
      181 attacttacc ttggaaagtg tttccttcct gtatggagat agaatcatcc tgcaagatgt
      241 atcctttggc attcacaatt cagaaaagat tggaattgta ggcattaatg gaagtggtaa
      301 aacaactctt ttaaatatca tcggtggtat cattaagccc agtacaggga ttgttacttc
      361 aagaaaagat ctaaaaattg gttttttaga acagaatccc cagtttattc cagagctatc
      421 tgttttacaa cacactatac ctacccaagg agaaataaaa gatgaatatc attataaatc
      481 aatattaagc cgtttaggta tagaagatta caatcaaaaa atgattttat tatcgggagg
      541 gcagcgtaga aaagccgatt tggcaagggt tttagcttta gaaccagatt tattgatttt
      601 agatgaacct accaatcatt tggatttgga tactatagaa tggttacaaa actatttagc
      661 ggatacgaat aaagccatta tttttgttac tcacgatcgc tattttttgg atgcagtatc
      721 caccaaaatt atagaaattg agaggactag actattttat tacgagggaa actatagtgc
      781 ctacattcac ggaaagctaa ttagagctac agacagtcaa cgaaaagaaa cacgccgtca
      841 ggcacagctt aaaaaagaat tggattggtt aaaccggggt gccaaagcac gaaccagtaa
      901 acctaaaaac catatagatc gcgtaaaaga gctattaagc aaaagctacc ttatttccaa
      961 tcaagatttg gatatatctt ttcaaataga tcgaatgggc aaaacgattc tggaattgca
     1021 tcgcttaagc aagtcctatg gaagtcgagt tcttttcgaa gatcttgatc ataattttca
     1081 aaacagggaa cgcattggga ttataggtcc caatggctgt ggaaaaacaa ctttactgaa
     1141 aataattgca ggagaagatg aaccggatat tggtgttgta aaagctggta taaacaccca
     1201 ttttgcttat tataaacaag atgaagatct cttcgacccc aacactacag tttatgaata
     1261 catcgctcaa tttgcagaag tgattaaaac tgagaacgga aataaaatta ctgccgcaga
     1321 aatgcttaag cgttttctct tcgatggtaa aatgcaacaa atgaaattgg gttcattatc
     1381 cggaggtgaa agaaagcgtc tctatttact caaagcactg atgtttggtg caaatttcat
     1441 tattatggat gaaccaacca acgatttgga tattcgaact ctggaaattt tagaggacta
     1501 tctggatgcc tttaagggct gtctattaat tgtatcacac gatcgtttct tcttggatag
     1561 aacagtggat taccttttta tttttcagga aggaaagatt cgtaaatttg ccggtaatta
     1621 ttcagattat ttgctggtaa agcgttttga gcaagaagaa gatacaacga agaaaaagcc
     1681 tgctatttta cgtcccaaac gaatttccaa gggtcttacc tataatgaac aacgagaatt
     1741 ttctcaaacc gaaaaagata ttgagaagat agaaaatcag ctcatagaat tggaagataa
     1801 gctaaatgat ttttcccaga ctcttactcc caatgattat catcaaataa gcataacaat
     1861 acttaaattg gaagaacaac atcaaaacct tcttaaccgc tggttggaac tgagtgataa
     1921 gcaagacaac tgatttaata tacaacctat caaatatttc cttaaaaagg aggtacaaaa
     1981 tgatatcaga attagtctac cgcaatcgta gttatcgtag gttttacgaa tcaaaaagga
     2041 taactcactc tcagcttatt cagattatgg aaacggcaag atttattccc agcgcaggta
     2101 acttgcagaa attatgttat tatctgattg aagagcgtag tgaatgtgat aaaatgtttc
     2161 cacatttgaa atgggcaagt tacttgaaaa aatggaatgg acccgaacca ggagaaagac
     2221 cttcagctta tataattatt ctggttccca aaagctgttc caatatggtt tatatcgata
     2281 ccggtatagc agctcagaca atactcttaa ccgctgttga taatggcttg ggaggatgtt
     2341 tgttggcttc tgtcgataaa gatgctgttc ataaaatatt cttgctgccc gataatatgg
     2401 atatcgtatt agttatagct cttggttatc cagctgaaac agttgtaata gaagatgtta
     2461 cagaccagga taagattgaa tactggcgcg atgaaaatac tgttcatcat gttcccaaac
     2521 gcaaattgag tgatcttatt ctaaattact gatcgtataa agttgccagg tattctagtt
     2581 ataattgaca aatatagata gtttgggata gtggtttagt tatggaaaat gtaattgtat
     2641 acagcgttta tgaagttacc aggcagattc gtcaggttat tgaatcttca attgaacctc
     2701 tgtatgtgat gggtgaaata tcaggttttg ttcatcatag ttcagggcat atatatttta
     2761 atctgaagga cgaaaattcc tccctacgct gcactttttt caaaggggcc aattataatc
     2821 taactttcaa accggaagat gggatgcagg taatctgtta tggtaaattg accgtttatg
     2881 aaaagggggg aacttataac ctgaatgttt taaaaatgca agaagctgga caaggacttt
     2941 tacaacagaa atttgaaata ttgaaaaaga agctggaaaa ggaaggtctt tttgcaacag
     3001 cacataaaaa accaattcct aaatatcctg agcgcattgg aattgttact tctccaacgg
     3061 gagcagctct tcaggatata ttaaacatta tcagccgtcg ctatcctgtt gagataattg
     3121 tttatcctgc attagttcaa ggtttggaag cccctcccca aattattaat ggtataaaat
     3181 acttcaatac aactaaagat gtggatttta tcattcttac cagggggggc ggttcgcaag
     3241 aagatctatt ttgtttcaat gatgaatcat tagttagagc tatattcgat tccgatttac
     3301 ctataatttc tgctgtagga catgagattg attttacttt ggcagatttc gttgctgatt
     3361 tacgagctcc aactccttca gcagcagcag aattagcagt tcctgataag aaagacctgc
     3421 taaacctgtt aaattcttta aagaaaagat tggacttaac ggttcaaaac aagctggaaa
     3481 tgaaacataa accaattatg aattatcaac tacagctaac ccaataccat cccgaaaagg
     3541 tatggcgaga atatcagcaa cgttttgatc tggcagaaat ggctatgaaa aatgcactaa
     3601 aacaacttcc agataagcgt ttacagtttg agatgagaac ccaaaccatt atgcagcagg
     3661 taacaagcaa gattgatctg tgtatagccc aagctaaagc gaacagtaat ttagcctggc
     3721 atcaattaaa tgacaagaca ctaaatttac ttcacaataa agccaatcac ttaaactctt
     3781 taggcaacat tctggagcaa ctttctcctc gcagcattca aagaaagggt tggataatgg
     3841 cttttaaaga tggaaagcta attagttcgg ttaaacaaat taccaaaggc aatcatctta
     3901 atctcgattt tattgatggt atagcagatt ccataataga aagggtggaa aataaagagt
     3961 gaaaagagga ttattgatat tttcattact gtttctgcta aagttgagtg cagaaattcg
     4021 ttcagtttgg gttttacctt gggatataac gaccgaaagc gctatagatc aagttattaa
     4081 cacagccgtt gattgtaacc aaaatgagtt gctagtagaa gttcgttacc gggctgatgc
     4141 attatttgat acttcccaag gtgcctatct atatcccaat cctgaacctg taagctatat
     4201 attgaataat tcatcatttg atccgttaac ttatataatc caacaagcac ataataacgg
     4261 cttagcagtt caggcttgga ttgtagtttt caatgctact caaactgatc actatcttat
     4321 tcgacagaat tatatctata acaatcatcg caattggatc acttacgata aaaacggtta
     4381 ccaaatgaat aatggatacc gttatggtta ttttatcgat cctggaattc ctgaagttca
     4441 ggaatatttg ctaaatattg ttggaaatat agcagctggt tatcctgaat tagatggtat
     4501 tcatttagat tatatccgtt atccggatgc ggatttaggt tatcatccta tttcccaaga
     4561 actttataga gcatattgcc taaatcagat ggatatcact tataatgaat ggcggataat
     4621 gcaaatcaat aattttgtag aaaaggctta ccacctaatc aaagaaatca atccttcttt
     4681 attattaacc gctgccgttt ttgctgatat tgccgatgca aatgtatctt atgctcaaga
     4741 ttggcagtcc tggcttaaga acggtattat cgatagggtt tatcccatgg cttatgatat
     4801 taattatgca aaattcagca aacacacaga acaaatgaaa ctgttaaatt ttgattcaaa
     4861 aatagtgatc ggtttacgtg cctggaatga aaatggttct tctcttgccc ttggtaaagg
     4921 agcttcatat aatgttcaga atgtagctag aaagatagat ttagccagag agcaaggatt
     4981 tgcaggcata tctctattca gttattcagg tttaataaaa ggaaatgcct gggcgcagct
     5041 taccaggcta agttattctg agaaagcaga atatcctttt gaaaaagtca gtactccact
     5101 ggttaatcaa tctgttacag aattaaaaca acctccggat gtaaagcttt tacgattaaa
     5161 caatgaaatt cttatacagc ttcaaatacc aagtgaggga ttatggaatt gggaacttta
     5221 taaagagagt tttttgctac agcataatag atattatctg aaagggaata acattgatat
     5281 cttgaatgtt ataaacccta atatttgtgc tagtatcgaa ccgggacagt atatagttca
     5341 actctatcaa gaaaattctt ccttcaaata ttttattcca gtagaactga aaatagccaa
     5401 tgagtgatct tccaaaacag gcaggtattc tttccgctac tgaattcttc cgtttagcaa
     5461 tcaaatctat aattggaatt gctttagccc gcataattac tccagcagaa ttaggtagtt
     5521 accgtcaact ttttttgatt tatactactt tctctactct tttattgtta ggaattccgc
     5581 aaagtatgct atattttctt cccaaagcag aaaatgcgga aaagcaaagg ttattgataa
     5641 cccgcagttt gaacctgatt tctattttag cactcatttt tgccttgtgt ttgcttattt
     5701 tccggaattt tattgctcaa atatttcata atccccaatt gggaaatctg ctaattatct
     5761 atgctctata cccgttattt ctattcgtaa cacaattata tagctctata atgatgggtt
     5821 tgaaacaacc cttgagagca gcaaaattca ctatcttttc tgtgctttcg gattttatct
     5881 ttattgtaag tgccgctttt tttacccgca atcttactat tataatctgg gcagtgattg
     5941 cttctgcttt tctgcaatgg ctttatgccc aaattactct caacgaattc aaagaaaaaa
     6001 aagttcgctt tgattttgcc tcagttaaag aacaaatgaa ttatgctttg ccacttggtt
     6061 tatcctctat tattggaatg ctcagtatcc aattagataa gttaatgata tccggttttt
     6121 tcccgccgga aaagtttgct gttttctctt taggagcaac ggaatttccg attgttggca
     6181 tattagctaa ttccatcaat tcagttttgt taccgcattt gagttctacc aataccgcaa
     6241 ctatgggaga actttatagt ggtgctgttc gaaaaaatgc cattctggtt tttcctttaa
     6301 tggctttatg ttttattttt gctgaaccga tctttatatt attatacggt aaaatttatg
     6361 ccgaagcggc tgtttatttc cgcatctatc tgtttttaat gcctttacgg attgctactt
     6421 atggcatttt gtttcaagca ttcgggaaaa ccaaattcat aatgtataat tctctctttg
     6481 tgcttattgc caatgctgtg ttaaattata tcttaatcat caatttagga atgaaaggtg
     6541 cagcaattgc aacagttatt gtcacttggc tatcggttat gatttacctt atccagattg
     6601 ctaaggtctt aaaattaaag ttgctggact ttttcccaat tggaaaaata acaaaaaatg
     6661 cccttgtaac cattatttcc ctctttctca gttttctgat aatatggctt gggaaacaaa
     6721 atcttctctt tgtagccatc ggaggaattg tgttcatttt gagctattta ctattgggta
     6781 gaaaaaccgg agttattttg gattatgatg ttcaacttgc caaagaactt atcaatgatc
     6841 tgaaagggaa gttcaaaaaa tgaattatta tgctgaatcc acctggaaaa attacgatcc
     6901 tgaacagcaa gcagatataa ggattgccaa gcttttatca ctttttcccg aaaatatgca
     6961 ttattataaa tataccaatt gtggcttaac atacaatgct tatgggcatt tacaatcctt
     7021 ttctaaaaat actttgaatt ctcttttaac tccggaattt tcttttacag cccaagatat
     7081 actctgtcct ttgaataggg attttattcc ctttttactt gatattaaac agcattggct
     7141 aaaacaatgg tttaatcctc cttcaggaac tatcgattcc aactgtggaa aaagtgaatt
     7201 cttaaataaa aaatctctgt tcacgaagtc cattaattac tttattaata tgtcaacgaa
     7261 gccacagccc tattggttta tggaattgtt tgtctggcgg gagaattgaa aatgaaaaat
     7321 ttactgttta ttacttatca ttttcctcct acaggcggtt ccggagtcca aagaggactt
     7381 aaatttgcca aatatttacc gcaattcggt tttagaccaa tagttctttg tgccgattat
     7441 cgctgcttaa aacaacccaa ggactatact ttgctaaaag aattaccccc cagtgtaaaa
     7501 atttatcgca ccttcacttt ggatgcgaac tggtttttta agctgctgtg gggattaaaa
     7561 ttaaataaag tagtcacctt cttgcaacgg tatatcttga ttccagatac agaagttctg
     7621 tggctacctt ttgctttctt aaaattaaga aaaattcttg atgctaatca tattgattta
     7681 gttttaattt ctgctccacc ctattcctcg ctatttttgg ctaaatattg caaacatcgt
     7741 taccagctaa aggtctgtgt ggattttaga gacccttgga gttttgggat aggcagaaaa
     7801 tacttaaaac cacccagctg gatagcaaat aaagaaaaca attgggaaaa agagattgta
     7861 actttggcag ataaaattat ctgtgtaaac gacaaaatgg ttgatgaatt caggcaactt
     7921 catactaaaa ttccagccgt taaattttgc agcatcacca atggctatga cgaagaggac
     7981 ttttctatat ccactcccct aaaaaaaagc gataagttca aaattgtata tactggtagt
     8041 ttttatgatg ccagaagacc gttaattctc tggcaggcat tatgggaact gatagaagag
     8101 ggaaaaataa acccgcaaaa aatcaccgtg aatatttacg gtagaaatac taaaaatttc
     8161 gttttaggcg aatatcagga taatctcctt atccaaaaga ttgtgcattt ctacggctat
     8221 actccgcata cacaaataat aaaaattctc ttatctgccg atgtgctttt attatattct
     8281 ggttttggca atactgaaca gctgaatagt ccagccaaat tatttgaata tttacgcagt
     8341 gggaaacccg tttttgccat tatagatcca gaaagttccg cggctgaaat acttctacca
     8401 gcaaatacta tttttcttgc taattccgct tctttaagtt ccataaaaaa tacattatca
     8461 gaactatata ctcaatggga agaaggcaaa ttgaaagtta gcccagattg ggaatatatc
     8521 aaaaattttg aacgcaaagc cttaaccgct aggcttgcag aagtactatc ttctataaca
     8581 taatgaaaat cctcattatc ccttcttggt atccatatcc gaaaaaacct tatgcgggaa
     8641 aattctttat agaacaggca aaggcattat ctgagaacag caactggcaa tttgaaatac
     8701 taaactgggg acagaatgaa ttccaaatgc aaattaggta tcctctatct tcaattgcaa
     8761 aattattcgc ttatttaaaa gcaaaacctt cctctcgtaa tttcaattct aacttaaaag
     8821 agctattcat cccacacatc acctggacta gtaaactgct atcgggaaac ataaaaaccc
     8881 tgataaataa aatggaatta attcccaaac cggatataat tcattcccat gtaacatttc
     8941 cagctggctt tgtagctatg cttttcgcag aaaaatggaa aatcccatat attatcacag
     9001 aacatagcgg tccctttccc tttcctgaat ttgtttctaa taacagaatc tcgaagaaaa
     9061 ttactcttcc tcttcaaaaa gcagcagcag ttatatctgt aagtagatat cttcaaaaac
     9121 agattctatc tacctgtttg gtaaaaagta tagttatccc caatttgatt gatacagaat
     9181 tctataaacc agtttctata tcagaaaaaa gaagacgctt ttccttcttt tctttaagtg
     9241 cactaattcc tgccaaaggt gttttagatt taattgaagc tttccgacta gcagtaaata
     9301 aaggtatgga tgctgatcta tatttaggtg gatctggtta tttagcaccc aaattaagaa
     9361 agctgattat tcaataccat ttggaaaata ggattcatct actaggatat cttacacctc
     9421 agcaagcgct tgaacaattt aataaatgta attgttatat cttaccaagc catatagaat
     9481 ctttcagtat ggttttactg gaggcaatgg cttgcgggaa accaattata gctacagatt
     9541 gtggtggacc aaaagatata attactgaaa caacaggagt ccttatccct cctcaaaaac
     9601 atgatgttat ggctatagaa atgctaaaaa tggccaataa caccaacaaa tataaccctg
     9661 atagcatcag agaatggtgt ttaaaaaatt attctcctca tataatctgt gctcaaatat
     9721 caagagtata tgaaagtatt actggaagtt aaataatctt ctaactgttt aggacttttt
     9781 caataacctc aatcatctca ttgcgcataa atggcttttc caatactgct ttagctccaa
     9841 gttttaatgc cagaggaaga tagctgtatg gaccgtattt tcccccacca ctcatcgcga
     9901 tgattttgat tccaggtttt atggccatta agtcctgaat ggtctccaaa ccttctttat
     9961 taggcataat gatgtccgtt ataactatat caggatcaaa ttcacagcat atttctaatg
    10021 cctgatcgcc atcacctgcc tgctgaacaa agtatccaga attttttagc atttgtacta
    10081 aaacattgcg gaaacttaca tcatcttcaa caactaaaat tttagtcatt aaatatcctc
    10141 gtttatgtaa tatatattta tagtaattag ttaattaaaa ctaggttgga accttaattt
    10201 ctatcccgtt tcattgtaaa ggagaattct ttagataaat agaattttga aataaaaatg
    10261 tagaatatta tccacaatgc gtaatctaaa agggttttca agtgcaaaaa aatggtggcg
    10321 aaacccagaa tcgaactggg gacacaaggc ttttcaggcc tctgctctac cgtctgagct
    10381 atctcgccaa ccattttcct ctttaatttt accagctttt tctgtcaaga ggaaattatg
    10441 ttgactttct atcttattgt tttgtaatat gttaacgaaa tgttctttcc ctcttggata
    10501 gctctatatt aaattcaaat tgattgataa gcattatttg ggcatagata agactattct
    10561 ttcaaacttt agaaactcgt aaaatactat accattgctt tataattcta taatttcgtt
    10621 agaggatcgt taaagaatct aataacaaat gaactattat ctgctgcaaa gtaaaaatac
    10681 tatataaaac cgttgaacta ttatgagtta aggcaaaatt ctgataatct atgacgaaat
    10741 taggagagat atttgaaaaa cttcaatgtt tttttctgct gcttgatttt tagtttgtac
    10801 acaaaaaaac ctgaatgcca aggtttctac ctcttcaagc tatggaacag aatgaaccag
    10861 gcagattagc atattctcct caatatcatt gacgggtttt acttctatgc actaaataat
    10921 agttttatct ataatggaga gtggagaaac ggcatcttac aaaataaacg gttacttttc
    10981 tcattaaatc atagctctta ccgacaagag aagattatct tcaaccttgc atcatatagt
    11041 atatcactca ttatactagc cttaatcaag cgttaattat taaggagtga ttaagggatg
    11101 ataaaggagt gatagacact tcccagtaag gagctagttt gggataataa tggctagtaa
    11161 aagtataagt gatcactgaa aattgcaaat ggcagttcta caggctaatg ttgaagctta
    11221 taaataaacc gtggggagca ccgggtggtg tccccacgaa tcggagggca ttctatgtag
    11281 ggtctgtagg ctgcattcaa tgtgagattt ggcaggattt ctgtcaatga agattcttgc
    11341 agattcttta tcatattgag tgggaaggac ttgccccctg ttgcgagatg tgtatagtag
    11401 accaataaac aaacagtcgg gtaaccgaga agaaaatcaa gatgaccaag caaaacaaga
    11461 atcgtagaga gcaagcaacg ctggatcagg tggcagcttc cggtagtcag atcgtactta
    11521 agaggaaaga tggttcggag caaccaattg aaaagaatat cgaatcccag cccagaggat
    11581 aagtcaaaca atggacatat aactattaca ataaactgaa ataaaaagct atacaaaaat
    11641 ggtggtctcg caattatggg atcattaaaa cataaggaga ccaccatgtt attaatacag
    11701 aatgaaacca ccgaattcgt gaaagaaaaa ttgtcaagct tctgtaaaaa aaatatggga
    11761 atgccactaa tttcagtttt tcaaggcatt cccacaaaaa agtgatgtgg acaagttata
    11821 acacactgtt g
//
LOCUS       DAOSTH010000048        11784 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_5414_length_11784_cov_15.092335, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000048 DAOSTH010000000
VERSION     DAOSTH010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5414_length_11784_cov_15.092335"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1150
                     /locus_tag="PLB65_06160"
     CDS             <1..1150
                     /locus_tag="PLB65_06160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78148.1"
                     /translation="NSGEFTPHTFIMAAGASAVYTVVLDHYTWVPVDFAVTNIASDLS
                     CEFLGTQVPYDIPADEPTVLPPDIPIPPEVTLQILIETVGTGGAYIDPIPLGDLPEWV
                     NPAFTPTVYFTMDLIGLGPWTITFTTSTSCWVAYYLGGNWHTVQSTPVGTDFIVTFTI
                     DTPPKDISDIPVVLGDSDPTLPVELSTFAATITAENYVNICWITQSETGMTGFNVYRN
                     VNNNNLSSAHKINPNLIDATNTSSTQIYNLIDKEVENGNTYYYWLECVDRSHNSTFHE
                     TSPVYLDYITPPILPEYTTMRNAYPNPFRAGSGTTIEVDVKAGDNGTVTVYNILGQVV
                     KTFPVAEGINNLTWNARDSKGNLCGNGIYFYKLSTKTINQTKKMVIIK"
     gene            1593..2690
                     /locus_tag="PLB65_06165"
     CDS             1593..2690
                     /locus_tag="PLB65_06165"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HOQ78149.1"
                     /translation="MENRIYEYLSVFIMAWLFVYGLTPFIIRLASALNFVDKPEARKI
                     HLKSVPLLGGLSVATGFVLLCIYDVLISPGRFLDEPMLGYLAGSVLIVIIGLIDDHRG
                     MKPVLKMFGQLVVSLLFIFTNFTLAELSLMFGSIYISLPIMLLWMVGLMNALNFLDNM
                     DGIISGMAGILGLGFFAFALINTTQANQEAMALMALIALSFAGSAFGFLPYNFNPAHI
                     FLGDAGSMFIGYFLSSMGILMARYAANRYNDKFFYLLPVLLLSYAIFDISLVSYTRRR
                     DGRQVMQGGKDHSTHRMNTILGSIKITAIMIYTINVLIVLTSIIIFRIGNRQLLIITT
                     AIFAAFFIFFGRKLDKVPIVVPANQQKHKTE"
     gene            2693..3430
                     /locus_tag="PLB65_06170"
     CDS             2693..3430
                     /locus_tag="PLB65_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ78150.1"
                     /translation="MEVTILGSGSGLPQLDKHLSSILIRQANKLFLADCGDSCSHRLL
                     EYGIDADQLDAIFVTHYHPDHLGGLFMLLQMLYLQNRSRDLLLFLPERLASFLEIMQL
                     MYTYPQKFGFNLLIRSIEEVELYYDWISAVPNDHLYRYAPIITEYHLPNQMKSWSLCF
                     NVANGKFVYTSDLETTDCISDTLKGAHTALVDAGHPQAEQILKLEYAGIQRIILTHGI
                     NPELENRKNELNPEIFEFAREDFVYKI"
     gene            3414..4826
                     /locus_tag="PLB65_06175"
     CDS             3414..4826
                     /locus_tag="PLB65_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78151.1"
                     /translation="MFTRYRLIFLLLLSSLLLLTGCEINRLKSAEDLYQKHLYATAIQ
                     ELDDLIANAENRAIATKAEILRSNSYIALAEAAVEKKNWPLAIRFFKLSNSDAANFRL
                     AEIYRDLAVEASKAGNLELSKIYLDTIYREVPETPIIPEVLYRRIAYYVDVKADNEAA
                     WADYMQLYDNYPNNPYEVLARNYVIRFIPGKLDYAAILNEQGYYTDALKELFEISKYP
                     VVDMDRLNKQISDVYERQAEELINEQDYVEADRLFRIALQYYPQKKDSITNRLESITN
                     LFIQKGNSLLEAGDYEAALIHYRKTFDIIPDYPPAVEAIKRVYTIQENIRKAGELFSQ
                     AERLEATGKLNEALQTYNAAYALDPKPEYKQKAAIVQNIMEANKNPSAFARRILNEYR
                     GGILNTRIQNQKKELLKRYKENEIQDSGWKLMLSTGQYKYEARYDLLTPNETYLYVWQ
                     INLRDRSIVPLNKLSEALMQ"
     gene            4823..5425
                     /locus_tag="PLB65_06180"
     CDS             4823..5425
                     /locus_tag="PLB65_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78152.1"
                     /translation="MKAINKILLAVALCLLMEIVAAPIKAQSFDIQAFSDTTKYGWKN
                     YLDRNAYREDLKQRQELLQIYEMEAQPLNTNILKSALIPGWGQFSTKESTKGTVILGA
                     EIVLAGGAYYFMDRAMSKYRLYEKATQVDDIEKYYKDAQVPYQYSFIMIGFAGIIWAY
                     NIFDVIQSTQDYNVRLWEEIIERTQRGPVNITPTGVEVRF"
     gene            5426..5845
                     /locus_tag="PLB65_06185"
     CDS             5426..5845
                     /locus_tag="PLB65_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78153.1"
                     /translation="MSSRWLIVIIILFACFFSSCDLKRSNPLDPNGEGDIVIPDPVTG
                     ISYTATADQDLRVVTIRWVANSPYNTDGYYVYRGIGYNSSFALVGTVTINEFTHSSAN
                     DPSIQPGYYWYRVSAYKVYPEGKLEGRRSEPFSVKVP"
     gene            5876..8023
                     /locus_tag="PLB65_06190"
     CDS             5876..8023
                     /locus_tag="PLB65_06190"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOQ78154.1"
                     /translation="MNNRQLEVVTDTEYPVLVLAGAGSGKTRSLIYRTAYLIKEKHIH
                     PWNILVVTFTNKAARELQDRLEKLLNIPMRTLWVGTFHSLCCRILRFESAHLPFNSNF
                     SIYDEDTQVSLLKRIYKEYGFDKQKFPINRMLNRISRYKNKLMLPEDIQNPDDEKDSE
                     FATFINIYRLYQQQLLLNQALDFDDILLWTAKLLISNNAVREKYRSQFQYVMIDEYQD
                     TNKAQFEIVYQIAAGHQRVCVVGDDDQAIYSFRGASLRNILEFERDYTNVHSIRLEQN
                     YRSTGNILALANAVIANNRRRHNKELWSELPTGEKPILAVYENEIEEANKITEEVVNL
                     QNKGIPLREMAVLYRTNAQSRVFESALIQAEIPYSIVGGVQFYQRKEIKDLLAYLYVL
                     SNVNDNESLLRIINEPTRGIGQTSINRLISFANKTQMSLYKALQYADAVEELKNPARN
                     RISEFYLLMERWHNSITQKPALELVKTIVEELELIERYKNSNDPREIARAENIVEFVT
                     SVSEFSEHFYRENDRLPLIEDYLPYVALQTDMDRMKEEVDSLRLMTLHNAKGLEFEMV
                     FIVGLEDELLPHRMSMNTRENIEEERRLFYVGITRAKNYLHLSYAKTRRLYDTFYFPV
                     RSQFLAEIDSELYYLTQGDGGIAAPPKRMKKAGKVITENQKHYKIGQKVYHSEYGIGV
                     ILSVDGDGPDARLTISFGNRLAKIIGSYVEVTK"
     gene            8179..11601
                     /gene="mfd"
                     /locus_tag="PLB65_06195"
     CDS             8179..11601
                     /gene="mfd"
                     /locus_tag="PLB65_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOQ78155.1"
                     /translation="MLYSKIEEQLLQSEFMQGVQKLPLKDRSIQIYHLNQSARALIAA
                     HLWKQTGKNVIIVSQDDIIAEEIWDDLCVLIGKENAYYLPDYEILPYEERSPHYSIRA
                     TRMETLLSTFQNSTSGIYSLSIRALGRFLPSRDLLGRHILHLQQGMEYDPDVLLHKLY
                     DMGYDIQYQVSKVFQAARRGGIIDVFSPPLIHPVRLEFWGDEIIGMRLFSIATQRSVE
                     EYLTEFTILPARELAISDINSGSPIIAKIREKGFFEGIENYYALLCKELQTFAGYFEK
                     DNRILVWNNYYYIREEYETLFEQAISTWQKEAKIQGRARIPLPEKMFADLDTLTKIKS
                     GSDNLYLSQSEFELSFPTQNLRAPIESQPNFESDLGFLTKTLMEKQKKGWNNILLFDN
                     QSQAKRMQQTIYPSSENRNPSEQLPEIPFTEYIGVLHEGFSIEDCRLILYTDHEIFNR
                     YKGKRYTPRYAPGETIVNYEDLKPGDYVVHIDHGIGIFEGLKIISLDGSDVECLVLRY
                     ANDDRVYVPTYQLSLVTKYVAEESAKPVLNKLGSSKWNNTKRKAAQQIELIAADIVKL
                     YAERSSRIGIAHQADSEWQKEMEESFIYEDTPDQIRASKEIKEDMELPAPMERLLCGD
                     VGFGKTEVAIRAAFKAVCSGYQVAVLAPTTLLVEQHYRVFRERLIQYPVKIAMFSRFR
                     VPSEMKKDLLSIKSGSIDIAIGTHRLLSRDVQFKKLGLLIIDEEHRFGVRHKEKLRAL
                     QSNVDTLYMSATPIPRTLNMALSKLKEISLMQTSPKERLPVRTIITPRDMEVIKDAIR
                     REIDRGGQVFFIHNRVQTIETIASELRAAMPKVRFIVGHAQMPEQQLEKVMDAFLAKE
                     YQVLISTTIIENGIDIPNANTILIDNADNFGLAQLYQMRGRVGRSNRRAYAYLLISKG
                     TTTVARKRLEALTQYDYLGAGFQVALRDLELRGAGTVLGTKQSGIIQAIGFNYYNRIL
                     GNAIQAVENGETSNLFNDETPETRRKVKTEIDLYFPPGYIDDDEERLKIYRRLSELET
                     FEEIDEFEIELLDRFGQMPDQARWLLNYFKLSQLSNKLALRNCQVKHDNLILEFDAKS
                     LPSKEQLLHFISKIDQPFRFEAGKNLKVIIDLNPDANYLQQFETALSILQKW"
BASE COUNT         3720 a         2080 c         2344 g         3640 t
ORIGIN      
        1 aaatagtggt gaatttactc ctcatacatt tattatggca gcgggagcaa gtgcagttta
       61 cacagttgtg ctggatcatt atacttgggt tccagttgat tttgcagtaa caaacattgc
      121 ctctgattta tcttgtgaat ttttaggaac tcaggtacct tatgatatcc cagctgacga
      181 gcctacagtg ttacctcctg atatacccat accacctgaa gttacattac aaatactaat
      241 tgaaactgtt ggtactggtg gcgcttatat cgatcctatt ccgcttggag atcttcctga
      301 atgggtaaat cctgctttta ctcccacagt gtatttcacg atggatttaa ttggattggg
      361 accctggaca ataactttca caactagtac ctcttgttgg gttgcttatt acttaggagg
      421 taattggcat accgtgcaat caacgcctgt aggaacagac tttattgtca ctttcacaat
      481 cgatactcca ccaaaagaca tcagcgatat accagtagta cttggcgatt cggatcctac
      541 tctgccagtg gaattgagta cttttgccgc aaccatcaca gcagagaact atgtgaatat
      601 ttgctggata actcaatcag aaactggtat gaccggattt aatgtctatc gtaatgttaa
      661 taataataac ctgtcaagcg cacataaaat taatcccaat ctaattgatg ctacaaacac
      721 gagctcaact caaatttata accttatcga taaagaagtg gagaatggca atacttatta
      781 ctactggctg gaatgtgtgg atagaagcca taacagcact ttccacgaaa catctcctgt
      841 atatttagat tatattactc cccccattct accagaatat actacaatgc gtaatgccta
      901 tccgaatcct ttcagagcgg gtagtggaac aactattgaa gtggatgtaa aagctggaga
      961 caatggaacc gttacagttt ataacatcct tggtcaggtt gtaaaaacct tccccgtagc
     1021 cgaaggcata aacaatctaa cttggaatgc ccgtgatagc aaaggaaatc tttgcggaaa
     1081 cggaatctat ttctacaaac taagtacgaa aactatcaac caaacgaaga aaatggtgat
     1141 aattaagtag cgctatttag cgtttacaca ttcgtaatat aatatgatcg ccccggagaa
     1201 agaaacttcg gggcgattgt tttaaagagg agtggatgaa attagggaca atctcagggt
     1261 tcatattttt ataagatttc ctattaaaac ctgtattatg caatttgctt tcgtaataga
     1321 ataacaacat ttgggttttc tatattcctt gctaagtccc gtttatctgt cgtttatcaa
     1381 gccttaatca agcgttaata attaacgctt gattaaggga tgattagcgc ttgattaacg
     1441 ctttccatca agggcgaaga gcggagcttg catctgtata caattaaccc tatgctgaag
     1501 atagttattt ttttatttat ttcattcttc attcgttctt gacaaaaacg ctttcttttt
     1561 ttgcttggta agaaaaagta tggagataag caatggaaaa ccggatctat gaatacttaa
     1621 gtgtattcat aatggcgtgg ctatttgtat atggtttaac gcctttcata atccgtttag
     1681 caagtgcttt aaattttgta gataagcccg aagcgcgcaa gatacacttg aaaagtgttc
     1741 cacttttggg aggtttaagt gttgctacag gttttgtatt gctttgtatc tatgatgtat
     1801 taatttcacc agggcgtttt ttggatgaac cgatgctggg ttatcttgct ggttcagtat
     1861 taattgtgat aatagggctg attgatgatc atcgaggaat gaagccggtt ttaaaaatgt
     1921 ttggtcaatt agtggtaagt ttattattca tcttcaccaa ttttacatta gctgagctta
     1981 gtttgatgtt tggcagcata tatatttctc tgccgattat gttgctttgg atggtaggac
     2041 tgatgaatgc gctgaacttt ttagataata tggatggcat aataagtggg atggctggca
     2101 ttttaggttt gggttttttt gcttttgcct tgatcaatac cactcaagca aatcaggaag
     2161 cgatggcttt gatggcattg attgcactta gttttgcagg ttcggctttt ggttttttgc
     2221 cctataattt caatcccgct catatttttt taggtgatgc gggcagtatg tttattggtt
     2281 attttctttc ttcgatgggc attttgatgg cgcgttatgc tgcgaatcgt tataatgaca
     2341 aatttttcta tttattacca gttctattac tttcctatgc aatttttgat atttcgctgg
     2401 taagttacac gcgcagaagg gatggaaggc aagttatgca aggtggaaag gatcacagta
     2461 cgcataggat gaatactatt ttggggtcaa ttaaaataac ggcgataatg atttacacta
     2521 tcaatgtatt aattgtcctc acctccatta ttatcttcag gataggtaat cgtcaattac
     2581 ttataattac cacagcgata tttgctgctt tctttatctt ctttggtcgc aaactggata
     2641 aagttccaat cgttgttcca gccaatcaac aaaaacataa aacggagtaa atatggaagt
     2701 aactatttta ggctcgggat ctggtctacc ccaacttgat aaacatcttt ccagtatttt
     2761 aattcgccag gctaataagc tttttttagc agattgtgga gatagctgtt cccaccgttt
     2821 attggaatat ggtatagatg cagatcaatt ggatgctatt tttgtaactc attaccatcc
     2881 tgatcatttg ggtggattat ttatgctttt gcagatgtta tacttgcaaa atcggagccg
     2941 tgatctttta ctttttttac ctgaacgtct tgcttccttt ctggaaatta tgcagctgat
     3001 gtatacttat cctcagaaat ttggttttaa cttgctgatc cgtagtattg aagaggtaga
     3061 attatattat gattggatat ctgctgtgcc taatgatcat ctatatcgat atgctcctat
     3121 aattactgaa tatcatcttc ccaatcagat gaaatcctgg tcattatgtt ttaatgtagc
     3181 taacgggaaa tttgtatata ccagcgattt agagacaact gattgcatat cggatacttt
     3241 aaagggtgct catactgcat tagttgatgc gggtcatcct caagcagaac aaattttaaa
     3301 attggaatat gcaggaatac aaagaataat ccttacgcat ggaataaatc ccgaactgga
     3361 aaaccgcaag aatgagctaa atcctgaaat atttgaattt gcaagggaag attttgttta
     3421 caagatatag attaattttt ttattactgc ttagttcatt gttacttctt acaggttgtg
     3481 aaattaaccg attgaagagt gcagaggatc tttatcaaaa acatttatat gctactgcca
     3541 ttcaggaact ggatgatctt attgctaatg cagaaaatag agctattgcc actaaagctg
     3601 aaatattgcg ttcgaacagt tatattgctt tagcagaagc agcagttgaa aagaaaaatt
     3661 ggcctttggc aatccgattt tttaagctat ccaattctga tgcagccaat tttcgcttag
     3721 cggaaatata tcgagattta gcagtggaag caagtaaagc tggcaacctg gaattgagca
     3781 agatttattt ggataccatc tatagagaag ttccagaaac ccctataatt cctgaagttt
     3841 tatatcgtag aattgcctat tatgttgatg ttaaggcaga taatgaagct gcctgggcag
     3901 attatatgca attatatgat aactacccca acaatcctta tgaggtttta gcccgaaatt
     3961 atgtaatacg ttttattcct ggaaaattag attatgctgc aattttaaat gaacagggtt
     4021 attataccga tgccttaaaa gagctttttg aaatatccaa atatcctgtt gtagatatgg
     4081 atcgtttaaa taagcagatt tctgatgtgt atgaaagaca agcagaagag ctgattaacg
     4141 aacaggatta tgtggaagca gatcgtttat ttcgtattgc tttacaatac tatccacaga
     4201 agaaagacag tattacaaac cgattggaat ccataactaa tctctttatc caaaaaggaa
     4261 atagtttact ggaagcagga gattatgaag ctgcgttaat tcactaccgt aaaacctttg
     4321 atataattcc agattatcct cctgcggtag aggctatcaa acgcgtttat actatccagg
     4381 aaaatattcg taaagctggc gaactatttt cacaagcaga acggctggaa gctacaggta
     4441 aattgaatga agcgctccaa acttacaatg ctgcttatgc attagatccc aagcctgaat
     4501 ataaacaaaa agctgctata gttcaaaata taatggaagc aaacaagaat ccatctgctt
     4561 ttgctcgtag aattctaaat gaataccgcg gtgggatctt aaacactcgt atccaaaatc
     4621 agaagaaaga acttttaaag cgttataaag aaaatgaaat tcaagatagc ggctggaaac
     4681 ttatgctttc tacaggacag tataaatatg aggcacgcta tgatttactt acccccaacg
     4741 aaacctatct gtatgtgtgg caaataaatc tgcgagaccg tagtattgtc cccttaaaca
     4801 aactctctga ggcattaatg caatgaaagc tataaataaa attctcttgg ctgtagcact
     4861 gtgtttgcta atggaaattg ttgctgctcc aataaaagca cagagttttg atattcaggc
     4921 attttctgat actactaaat atggctggaa aaattatttg gatagaaatg cctatcgcga
     4981 ggatttgaaa cagcgtcagg aattactcca gatttatgaa atggaagctc aacctttaaa
     5041 tacgaatatc ctgaaatcgg ctcttattcc tggctgggga cagttctcca ccaaagaaag
     5101 cactaaagga acagttattt tgggagcaga aatagtcctt gctggaggtg cttattattt
     5161 tatggataga gcaatgagca aataccgtct ttatgaaaaa gccactcaag tagatgatat
     5221 cgaaaaatat tataaagatg ctcaagtgcc ctatcagtat tcctttatta tgataggttt
     5281 tgctggtatt atttgggctt ataatatttt tgatgtaatt caatccactc aggattataa
     5341 tgttcgttta tgggaagaga ttattgaacg cacgcaaaga ggacccgtaa atattactcc
     5401 tacgggagtg gaagtgaggt tttagatgtc ttcccgttgg ttgatagtga ttattatatt
     5461 gtttgcttgt tttttcagct cttgcgattt aaaaagaagc aatcctcttg atcctaatgg
     5521 agaaggagat attgtaatac cggatccggt tactggaatt agttatacag ctacagcaga
     5581 tcaagatctg cgagtggtaa ctattagatg ggtagccaat agcccctata atactgatgg
     5641 ttattatgtt tatcgcggga ttggttataa ttcttcattt gctttggtag ggacagtaac
     5701 tatcaacgaa tttacacata gcagtgctaa tgatccttct attcagccag gatattattg
     5761 gtatcgggtt tcagcttata aagtatatcc tgaggggaaa ctggaaggca gaaggtctga
     5821 acctttttct gttaaagttc cttaaacgat gtatccagaa ttttattctt cccagttgaa
     5881 taatagacag ctggaagtgg taaccgatac tgaatatccg gttttagtat tagcaggagc
     5941 tggttcagga aaaacgcgtt cgcttattta taggactgcc tacttgatta aagaaaagca
     6001 tattcacccc tggaatattt tagttgtaac cttcaccaat aaagcagctc gcgaattgca
     6061 ggaccgttta gaaaagcttt taaatattcc aatgcgaact ttatgggtag gaacttttca
     6121 ttcgttatgc tgtaggattt tgcgtttcga aagtgctcat ttacctttta attccaattt
     6181 ttccatatat gatgaagaca ctcaagtttc cctattgaag aggatttata aggaatatgg
     6241 ttttgataaa cagaagtttc ctataaatcg tatgttaaat cgaatcagcc gttataaaaa
     6301 taaactaatg ctgccggaag atatacaaaa cccggatgat gagaaagata gcgaatttgc
     6361 cacttttata aatatttacc gcttatatca acaacagctc ttactaaatc aggcactgga
     6421 ttttgacgat attttgttgt ggacagccaa gcttctaatt tccaataatg ctgtccgcga
     6481 aaagtaccgt tctcaatttc aatatgtaat gattgatgaa tatcaggata ctaataaggc
     6541 tcaatttgaa atcgtgtatc aaatagctgc agggcatcaa agagtgtgtg tagttggaga
     6601 tgacgatcag gcaatatata gttttcgggg agccagttta cgaaatattc tggaatttga
     6661 aagggactac acaaatgtgc atagtatccg tctggaacag aattatcgca gcacggggaa
     6721 tattttagct ttggctaatg ctgttattgc caataaccgg agaagacaca acaaagaact
     6781 ttggagtgaa cttcccaccg gtgagaaacc tatccttgcc gtttacgaaa atgagattga
     6841 ggaagcaaat aaaataaccg aggaagttgt caatttacag aataagggga ttcctttacg
     6901 cgaaatggct gttttatata gaactaatgc ccaatcccga gtttttgaaa gtgccttaat
     6961 tcaggcggaa attccttaca gcattgttgg aggggttcaa ttctatcagc gcaaagaaat
     7021 aaaggactta ctggcatatc tttatgtgct aagcaatgtg aacgataatg aatctctatt
     7081 acgcataatt aacgaaccca ccagaggaat tggccaaacc agtataaaca gattaatcag
     7141 ctttgctaac aaaactcaga tgtctctata taaagctctc caatatgctg atgccgttga
     7201 agaactaaag aatcctgcaa gaaatcgcat cagcgaattt tacttgctga tggaaagatg
     7261 gcataatagc attacccaaa aacctgcttt ggaacttgta aagaccattg tggaagaatt
     7321 ggaactaata gaaagatata aaaatagcaa cgacccccgc gaaattgccc gcgctgaaaa
     7381 catagtggaa tttgttactt ctgttagcga attcagtgag cacttttatc gagaaaatga
     7441 ccgcctccct ctaattgaag attatctgcc ctatgttgct ttacagacag atatggatag
     7501 aatgaaagaa gaagtggatt ccttacggtt aatgacctta cataatgcca aaggtttgga
     7561 atttgaaatg gtgttcattg taggcttgga agatgagctc cttcctcata gaatgagtat
     7621 gaacacccgc gaaaatattg aagaagaaag gcgtcttttt tatgtaggaa ttaccagagc
     7681 caaaaattat ttacatctaa gctatgctaa aaccagacgc ttatatgata ccttttattt
     7741 tccggttcga agccagtttt tagcagaaat agattctgaa ttatattatc ttacccaagg
     7801 cgacggtgga attgcagctc caccaaaacg aatgaagaaa gctggaaaag tgatcactga
     7861 aaaccaaaaa cactataaaa tcggccaaaa agtatatcat tctgaatatg gtataggagt
     7921 tattttgagt gtagatggtg atggacccga tgctcgactt actatatcct ttggcaatag
     7981 attagccaaa attattggta gttatgtgga ggttactaag tgaaagaact tcgttcagtg
     8041 agagtgctaa gccaataatt atcatcacca aagaaatatt tatgctgcct aagaactcat
     8101 ttagtgataa gggcgcataa tacctataat gaagaactaa aaaaatatac atccgtgtaa
     8161 tctgtgagag accataaaat gctatactct aagatagaag agcaattatt acaaagcgag
     8221 tttatgcaag gggtgcaaaa gcttcccttg aaagataggt ctatccaaat ttaccatctt
     8281 aatcaaagtg ccagagcatt aattgcagct catttatgga aacaaactgg taaaaatgtt
     8341 attattgttt ctcaggatga tatcattgct gaagagatat gggatgatct ctgtgtgtta
     8401 attggcaaag aaaatgccta ttatttaccc gattacgaaa ttttaccgta tgaagaacgt
     8461 tctccacatt acagtatacg ggcaacgcgg atggagacat tgctttccac atttcaaaat
     8521 tctacaagcg gtatatattc tctttccatt agagctctgg gtcgcttttt gccttcccgt
     8581 gatttattag gtagacacat attacatttg caacaaggta tggaatatga tcctgatgta
     8641 ttacttcata agctctatga tatgggatac gatattcaat atcaggttag taaagttttt
     8701 caggcagcaa gaagaggtgg aattattgat gttttttctc ctccgctaat tcatcctgtg
     8761 cgtttggaat tttggggaga cgaaattatt ggaatgcgtt tattttctat agctacgcaa
     8821 cgaagtgtag aagaatattt aactgaattt actattcttc cagcgcgcga gctggcgatt
     8881 agtgatatta atagtggttc tcctattatt gccaaaattc gagaaaaggg tttttttgaa
     8941 ggtatagaga attattacgc tttgctttgt aaagagttac aaacatttgc tggttatttt
     9001 gaaaaggata atcgtattct tgtttggaat aattattact atatccgaga ggaatatgaa
     9061 actctttttg agcaggcaat ttctacctgg cagaaggaag caaaaattca aggtcgggct
     9121 cgcattccgc ttccagaaaa gatgtttgcc gatttggata ctcttacgaa aataaaatcc
     9181 ggcagcgata atttatacct ttcgcaaagt gaatttgaac tttccttccc cacccaaaat
     9241 ctgcgtgccc cgatagaaag tcaacccaat tttgaatccg acttggggtt tctaactaaa
     9301 actcttatgg aaaaacaaaa aaagggctgg aataatattc tgcttttcga taaccaaagc
     9361 caggctaagc ggatgcagca aactatttat ccttccagcg aaaacagaaa tccaagtgaa
     9421 caattacccg aaatcccttt caccgaatac atcggggtct tgcacgaagg cttttctatt
     9481 gaagattgcc gcttgatttt atataccgac cacgaaatct ttaatcgcta taagggtaaa
     9541 cgctacactc ctcgttatgc acctggggaa acaattgtaa attacgaaga tctgaaacct
     9601 ggagattatg tagtccatat agatcacgga attgggattt ttgaaggact taaaataatt
     9661 agtttggatg gctcggatgt tgaatgtttg gttttacgct atgcgaatga tgatagagtt
     9721 tatgtgccta cctatcagct ctctttggtt actaaatatg tagctgagga aagtgccaaa
     9781 cctgttttaa acaaactggg tagctctaaa tggaataaca caaagcgtaa agctgctcaa
     9841 caaattgaac ttattgccgc agatatagtt aagctttatg cagaacgcag ttcacgcata
     9901 gggattgctc atcaagcaga ttccgaatgg caaaaagaaa tggaagaatc atttatctat
     9961 gaggatactc ccgatcaaat tagggctagc aaagaaatta aagaagatat ggaacttccc
    10021 gcaccgatgg aaagattgct atgtggagat gttggcttcg ggaaaacgga agttgctatc
    10081 cgagcagctt ttaaagccgt ttgcagtggc tatcaagttg ccgttttagc acctactact
    10141 ttattggttg aacagcatta tcgggttttt agagaacgct taatccaata tccggtaaaa
    10201 attgctatgt ttagccgttt tcgtgtaccc tcagaaatga aaaaagacct tcttagcatt
    10261 aaaagtggct ccatagatat cgccattgga acacatcgtc ttctttctcg agatgttcaa
    10321 tttaaaaagt taggcttact cattattgat gaagaacacc gttttggagt ccgtcataaa
    10381 gaaaaattga gagctctgca aagtaatgta gataccctat atatgagcgc aacccccatt
    10441 ccgcgcactt taaatatggc actatctaaa ctgaaagaaa tatcgttgat gcaaacctcg
    10501 cctaaggaaa gattaccagt ccgcacaatt attactcctc gagatatgga agtgatcaaa
    10561 gatgccatca gaagagaaat tgaccgcggt ggacaagtat ttttcattca taaccgcgtc
    10621 caaaccattg aaactatcgc ttcagaatta cgagccgcga tgcccaaagt tcgctttatt
    10681 gttggccatg ctcaaatgcc agaacagcag ttggaaaaag tgatggacgc ctttttggct
    10741 aaagaatatc aagtgcttat ttccacaacg attatcgaaa atggaataga tatccccaat
    10801 gctaatacta ttctaattga taatgccgat aattttggct tagcacaact ttatcaaatg
    10861 cgtggaagag taggacgcag caatcgacga gcttatgctt atctccttat ttccaaagga
    10921 actactacag ttgccagaaa acgactggaa gctttaacgc agtacgatta tttgggtgct
    10981 ggttttcaag ttgctttacg cgatctggaa ttaagaggag caggtacagt tctgggaacc
    11041 aaacaaagcg gtatcattca agcaattggc tttaactatt ataaccgcat tttgggtaac
    11101 gccattcaag cagtggaaaa tggagaaaca agcaatttat ttaacgatga aactccggaa
    11161 acaagaagaa aagtaaaaac cgaaatagac ctctacttcc cacccggtta tatagatgat
    11221 gacgaagaac ggctaaaaat ctatcgacgc ttgagtgagt tggaaacctt cgaagaaata
    11281 gatgaatttg aaatagaact gttagaccgc tttggtcaaa tgcccgatca agcacgctgg
    11341 ctgctaaatt attttaaatt gagccaatta tccaataaac tcgctcttag aaattgccag
    11401 gttaaacacg ataacttgat tctggaattc gatgccaaaa gtttgccatc taaagagcaa
    11461 ctattacact ttatctctaa aatcgatcag cccttccgtt ttgaagctgg aaaaaacttg
    11521 aaagtaataa tagatttaaa ccctgatgcc aattatctac aacaatttga aacagccctg
    11581 agtattttgc aaaagtggta agagaattag ttggctatta actatgcgcc aggttaaaat
    11641 tctgtcgctc aaatctgtcg tcccgctggg actttttttt agcattcttt ttatcgccgg
    11701 gttaaaaacc cgtcgctagt atgtatcgtt ccaagcggaa cttttttcat tatatatctt
    11761 tcttttcgag gggcttacgc cacc
//
LOCUS       DAOSTH010000049        11326 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_5653_length_11326_cov_11.646793, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000049 DAOSTH010000000
VERSION     DAOSTH010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11326
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5653_length_11326_cov_11.646793"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            11..86
                     /locus_tag="PLB65_06200"
     tRNA            11..86
                     /locus_tag="PLB65_06200"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:44..46,aa:Gly,seq:gcc)
     gene            127..795
                     /locus_tag="PLB65_06205"
     CDS             127..795
                     /locus_tag="PLB65_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOQ78156.1"
                     /translation="MHNIKAVIFDMDGTLIDSMQVWRKVDNDFLHSRGLEVPIDLFEN
                     LPAGNSFIQTAQYFKDRFSLPDSPESIMQEWTKMVSHHYETDIKLKSGARELIVALKD
                     RGIKIGLGTSNSLELARKVLSHNAIWHYFQCAITGDLDLKGKPYPDIYLLAAKSLQEK
                     PENCLVVEDTLTGVQAAKAAGMKVFAVYDEDSYPYHSMMKEIVDGFYPDFHILATELF
                     RLRK"
     gene            862..1554
                     /locus_tag="PLB65_06210"
     CDS             862..1554
                     /locus_tag="PLB65_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78157.1"
                     /translation="MKPQLYIIIGAYGSGKSEYSIHLARSLKAQGLDVVLADLDVVNP
                     YFRSREIRDDFILEGIDVIAPEGEYKHSDLPMISPRVKGALQNREKTVILDVGGDPAG
                     CRTLGRFEEIIKNRGYNMIFIVNTMRPFTTTPEEIITMKEALEATSRLQITEIVCNTN
                     LMEETTADIVLSGIKTVDKAAKQLSLPFDTYLVLSKYISIVPDHLYGKKREVMKYNLQ
                     KPWELLIARGIC"
     gene            1731..3746
                     /locus_tag="PLB65_06215"
     CDS             1731..3746
                     /locus_tag="PLB65_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HOQ78158.1"
                     /translation="MQERSIKRIILVCSVLCLLAAAAFANNNTKEENIDRGIRFTETN
                     PPVAPVRPIAEFEPASAVLIRYPLGIPLSLVVQLANTANVICIVSSSQQNSAISAFTN
                     AGVNMDKVSFINTITDSYWTRDYGPWFIFDGNGEYGVVDFVYNRPRPNDNLVPQIFAN
                     QLNLNYYGMNLQQTGGNYMCDGVNTAAQTNLVYSENGNNQTNVNTKMNQYLGITNYCV
                     VADPNNTYIDHIDCWAKFLAPDKILIRSVPISHPQYNAIETTATYFANQNCAWGYPYR
                     VYRVNTPNNEPYTNSLILNKKVFVPIVGSSNDNPALQVYRTAMPGYEVIGISQASSTP
                     WESTDALHCRTHEIPDKNMLHIVHTPWHSIISAGTDIVFNVEITAHSGQPLYTDSLFV
                     CYKVNSGDWQRSYLQPLTRNNYTTSLSGFSYGDSIRYFIHAADQSGRNADHPVFASLD
                     PHLFVIQPDTTPPNLVHNPITTIGNQTEPISFILSTNDESGINQVLFRYHIDNSAISS
                     FPMDPISEDTYIFQYYPEFGSENRSFFYSFAAYDSVNPPNIGYLPGVDLWYEVPIRPV
                     AITDEVQTPSIKEGLISVYPNPFQAGKEVLNLRYFTEKQSSVTCKIYNIRGQIIYENT
                     SVPKASGLQELVWNGKDKQGYASPNGLYLVELIQGSNRYKAKLIIVK"
     gene            4000..5187
                     /locus_tag="PLB65_06220"
     CDS             4000..5187
                     /locus_tag="PLB65_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ78159.1"
                     /translation="MEEIRLENLSKDFDGFVAVENVSLSIQSGELFSFLGPSGCGKTT
                     LLRMIAGFEIPSTGRILIGNTDITDLPPYKRQVNTIFQNYSLFPHMTVFDNVAFGLRI
                     RKFPKNEINDRVKEMLTLVKMEDQANKYPDQISGGQKQRIAIARALINKPKVLLLDEP
                     LAALDLKLRQHMLIELMNIHDAVGITFIYVTHDQSEAMSISDRVAVMNYGHIIQSGPP
                     DDIYERPTSVFSAYFIGATNLITGEVIGIEDDYVQIRCPDGTGNSFEIDSTFHFQPAI
                     GQELTISLRPEKIAISRNKPRYQEDINILRGVIEDILYLGSHTQYIVRVGDLRIRVFS
                     QHKRVYFDDTALDWEEPVWLFWHDTDTWLIDEIPENVLTADELSEGGINHFQRSLKRP
                     TAQ"
     gene            5184..6089
                     /locus_tag="PLB65_06225"
     CDS             5184..6089
                     /locus_tag="PLB65_06225"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ78160.1"
                     /translation="MKIIPENNPNYALQLATERKKNFTSWLLTAPALLWLIFFFLIPY
                     LIVIIYSFLTADIYDVKFEFTLEAYRQCLTPAYLHTLFLSFRLALLTTLLCLLLGFPV
                     AYYIARAKDKVKNTLLIFIIIPFWTNLIIRIFSWRIFLAYNGVLNNILLNLGLISQPL
                     EIMRTDIAVILVMVYVYLPYMILPLYSVLEKLDWTLLNAAMDLGANEIISFFKITLPL
                     ARGGIFAGGLLVFIPALGSYLIPQLVGNQKSLYLGQVITYKVKNIPRNWPMASALSLI
                     LLLIVALFLIMFYVLYSHSKSRRRV"
     gene            6086..6886
                     /locus_tag="PLB65_06230"
     CDS             6086..6886
                     /locus_tag="PLB65_06230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ78161.1"
                     /translation="MKRFSLTKLFKGLNLTSFTLVMVFLYIPILILIIFSFNDAKIIT
                     GWKGFTLKYYAQLLKNETIQIAFRNTMLIACLSTLISTILGVLTALGLENKKFSGSKF
                     VSILIYIPLVIPDILMGVSLALLFNFTKVNTGMLTVLIAHITFCISYTIIVIRSRLDD
                     FDYSLVEAAMDLGAKPSTIFWKIKIPLILPGIIAAALLAFTLSIDDFIITFFTSGRGF
                     DTLPIYVEGAIRRGTITTINSLSTIMIGLTLLLVVTTKRIRKIVISSL"
     gene            7107..8045
                     /locus_tag="PLB65_06235"
     CDS             7107..8045
                     /locus_tag="PLB65_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78162.1"
                     /translation="MNEIKIDKNSPNQFDTLPKGWVFYLLSGLNEELYAGYSANLKVK
                     LNYLYHKAMEGGIYQDMWNSVEKLNWVTYDRAISALIHCKCYLAENMPLYQYQIRPWE
                     EYVYLSLNSHRYPFIAIQEHTSDDWLYLGPFRNRFFLVDVLDIFSRILKLPACETGEY
                     PCDKWEQNLCQGWCLALADNAKINPEYSLDKLESLLKETFLVPENGVLELVQQERDRY
                     FNNLEFTKASLLNDEIELLSKYRNWLKFLIAAKHLNYNSEDLEIQKGKLTFCNFQGRK
                     YHFPIDNTEFRINETLALNLDTVDESKIIYDFLKLT"
     gene            8057..8938
                     /locus_tag="PLB65_06240"
     CDS             8057..8938
                     /locus_tag="PLB65_06240"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOQ78163.1"
                     /translation="MHNEISKLAIVKESHQGKVRDIYDLGENLLIVSTDRISAFDVVF
                     PDPIPDKGKILNAISAHFFKTTSNIVPNHFITDNVSDYPNELQKFKEYLDGRSMLVKK
                     TRVIPFECIVRGYISGSAWKEYKKTNTIGGMLIAEDMQESQKFTHPLFTPSTKVEDGH
                     DENISYREFMNRMDELLAVYIKDKSLELYSDAHDLLLKKGVILADTKFEYGTIGSQVL
                     LIDEVFTPDSSRLWALENYEIGKSPDSFDKQFIRDYLENSGWNKQPPAPSLPTEIIEK
                     TRQKYVTILKIITGKEL"
     gene            8941..9312
                     /locus_tag="PLB65_06245"
     CDS             8941..9312
                     /locus_tag="PLB65_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOQ78164.1"
                     /translation="MKKIICIVDDEEELVENLKIELEHLKPEWNILTFSEGMKAIQEI
                     VKGTVDLVVTDIAMPDMDGYELFWRIKDYDENIPVIMMTGFGYDPNHVLVRAKVDGLK
                     DVLYKPFETEKLVELIESHLK"
     gene            9313..10365
                     /gene="lpxK"
                     /locus_tag="PLB65_06250"
     CDS             9313..10365
                     /gene="lpxK"
                     /locus_tag="PLB65_06250"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOQ78165.1"
                     /translation="MDLNTFIQTHLLKRSPLSYALYPSGVLYGYIQKQCRTYSRRRAY
                     RAPCKIISIGNIISGGSGKTPLTISLANLLINKGLKVAVSHRGYKSAFENEPHIINAD
                     SGMLYDIHKTGDEAYLIASALLKVPVAVGRKRVLAVKLLLEKHPSLDVIILDDALQHN
                     YIHRDLDIISFDASIGLGNGFVIPAGYLREPLSNIPQNSLIIINRKNKAQTETLWLNK
                     INQLKIPIYHSSNTPKCFLDITGNVYPLDYLKEKRIVLVSGIANPASFADSVSSVGLS
                     YVKHYACIDHCDFKQPSLIENILELNPDYILCTQKDIMKLAKYESLQNRLLALKLEYS
                     FDQEDEFLNQVLNFIK"
BASE COUNT         3611 a         2033 c         2040 g         3642 t
ORIGIN      
        1 ctggtaccaa gcgggaatag ctcagttggt agagcgcaac cttgccaagg ttgaggtcgc
       61 gggttcgaac cccgtttccc gctccagttt tatgctccaa tttattttat aaggaagttt
      121 cattagatgc acaacattaa agcagtaatc tttgatatgg atggaacttt aattgattct
      181 atgcaggttt ggcgtaaggt agataatgat tttttgcatt ccagggggct tgaagttcct
      241 attgaccttt tcgagaatct tcctgctgga aacagtttta tccaaacagc ccagtatttt
      301 aaagatagat ttagcttacc tgatagcccg gaaagtatta tgcaggaatg gactaagatg
      361 gttagccatc attatgaaac tgatatcaaa ttgaaatcag gagccaggga attgattgta
      421 gcattgaaag ataggggaat caaaatcgga ctgggaacca gcaattcttt agaattggca
      481 agaaaggtat tatctcataa tgctatttgg cattatttcc aatgtgccat tactggcgat
      541 ctagatctaa agggaaagcc ctatccagat atatatttat tggctgcgaa aagcttgcaa
      601 gaaaaaccgg aaaactgctt agtagttgaa gatactttaa ctggtgtgca agctgcaaaa
      661 gctgcaggaa tgaaagtttt tgccgtttat gatgaggata gctatcctta ccattcaatg
      721 atgaaagaga ttgtagatgg attttatcct gattttcaca ttttagccac tgagctgttt
      781 cgccttagaa aatagattta tcatacctgg tttgaaactg aacaggtata tcttttatta
      841 gtgtatatta ggagctcgtt tatgaaacca caattatata taattatagg tgcatatgga
      901 agcggaaaaa gtgaatattc aattcatttg gcacgcagtt taaaagcaca aggccttgat
      961 gttgttttag ctgatttgga tgttgttaat ccctatttcc gttccagaga aattagagat
     1021 gattttattc tggaaggtat tgatgttatt gctcctgagg gagaatataa gcatagcgat
     1081 ttacctatga tttctcctcg tgtgaaagga gccctccaaa atcgtgaaaa gacagttatt
     1141 ttggatgttg gaggtgatcc tgcaggttgt agaacattag gacggtttga agaaattatc
     1201 aaaaataggg gctataatat gatctttatc gttaacacaa tgcgtccttt tactactaca
     1261 ccggaagaga taatcactat gaaagaagct ctggaagcca cttcccgctt gcaaattaca
     1321 gaaattgttt gtaacacaaa tttaatggaa gaaacaactg cagatattgt tttaagcgga
     1381 attaaaacag tagataaagc agcaaaacaa ttaagcctcc cttttgatac ttatttggta
     1441 ctaagcaaat atatatcaat agttccagac cacctttatg gaaaaaaacg cgaagttatg
     1501 aagtataatt tacaaaaacc ctgggaattg cttattgcaa gaggaatctg ttaatctgct
     1561 aactctggat catactatct caataattta tctcgccttg ctttgtaata agttctatat
     1621 ctttcgaaga tggtaagaaa ttaagtagta gacctttaaa aaaaaattgt gttttggcac
     1681 aaattatgta tattattata atatcactgt agaaacagga gtttatcttg atgcaagaac
     1741 gaagtataaa acgcataata ttagtatgca gtgtgttatg tttattagct gctgctgcct
     1801 ttgccaataa taatacaaaa gaagagaaca ttgatagggg aattcgtttt accgaaacta
     1861 atccaccggt agcgccggtt cgtcccatag cagaatttga acctgcctca gctgttctaa
     1921 ttcgttatcc tctaggaatt cctctttcat tagtggtaca actggccaat acagcaaatg
     1981 taatttgcat agttagcagt tctcaacaaa attctgcaat aagtgctttt actaatgccg
     2041 gtgtaaatat ggataaggtt agttttatta ataccataac ggattcttac tggacaagag
     2101 attacggacc ctggttcatt tttgatggca atggagaata tggagtggta gattttgttt
     2161 ataatcgtcc gcgtcctaac gataatttag ttccgcagat atttgccaat caactaaatt
     2221 taaactacta tggaatgaat ttacaacaaa caggtggcaa ttatatgtgc gacggggtta
     2281 ataccgctgc ccaaacaaat ttggtctatt cagaaaacgg taacaaccaa acaaatgtaa
     2341 acactaaaat gaatcaatat ttaggcatta ctaactattg tgttgtagca gaccctaata
     2401 atacatatat cgatcatatt gactgctggg caaagttcct ggcacctgat aagatattga
     2461 ttcgaagtgt gccaattagt catcctcagt ataatgccat cgaaactaca gccacctatt
     2521 ttgctaatca aaattgtgct tggggttatc cctatcgagt ttacagagtt aatactccca
     2581 acaatgaacc ctatactaat tccttaattt taaataaaaa ggtctttgtc cctattgttg
     2641 gcagcagcaa cgataatcct gctttacaag tttatcgtac agcaatgcct ggatatgaag
     2701 ttattggtat atcccaagca agttctactc cctgggaatc tacagatgcc ttgcattgca
     2761 ggacacatga aattcctgat aaaaatatgc tacatattgt tcatacccct tggcatagta
     2821 taatatctgc aggaacagat atagttttta atgtagaaat aacagctcat agcggtcaac
     2881 ccttatatac ggattcgctt tttgtttgtt ataaagtaaa ttctggtgat tggcaaagaa
     2941 gctatcttca acctcttact cgtaataatt atacaacatc tttaagcggt tttagttatg
     3001 gtgattctat tcgctatttt attcacgcag cagatcaatc tggaaggaat gcagatcatc
     3061 ctgtttttgc aagtttagac ccacatcttt ttgttattca accagatact acgcctccta
     3121 atttggttca taaccctatt accacaatcg gcaaccaaac agagccaatt agtttcattc
     3181 tttctactaa cgacgaaagt ggtatcaatc aagttctatt ccgttatcac attgataatt
     3241 cagcaatttc gtctttcccg atggacccaa tttctgaaga tacttatatc tttcaatact
     3301 accctgaatt tggttctgag aatagatctt tcttttacag ttttgcagcg tacgatagtg
     3361 ttaatcctcc taatataggt tatcttcctg gagtagattt atggtatgaa gtaccaatca
     3421 gacctgttgc tattaccgat gaagtgcaga caccctctat taaggaaggt ttaataagtg
     3481 tttatcccaa tccttttcag gcaggcaaag aagtgctgaa tttaaggtat tttacagaaa
     3541 aacaatcatc cgttacttgt aaaatatata atatccgggg acagattatt tatgaaaata
     3601 cttcggttcc caaagctagc ggccttcagg aattggtatg gaatggaaag gataaacaag
     3661 gatatgcatc gccgaacggt ttatatttag ttgagctcat acaaggttca aaccgctaca
     3721 aagcaaagct aataatagtt aaatagagcg atgttgatat atttcgcgta aaaaatatag
     3781 tgagtttgaa ttttcttctt gacaggaaaa caatatttaa atataaagat cttaagccta
     3841 cagaccctac atagaatgcc ctccgagcag tggggatgcc gcccggcatt ccccactgta
     3901 tttttttgct ttttaacaag aaggaaaatc ttttatcagt taggaaacta taggaaatgc
     3961 aaatacttat tacaaaaaaa ataggagcga aggacaaaat tggaagaaat ccgtctggaa
     4021 aacttaagta aggattttga cggctttgta gctgtggaaa atgtatccct atcaattcag
     4081 agtggagaat tattctcttt cttaggtccc agtggttgtg gaaaaactac tcttttgaga
     4141 atgattgctg gctttgaaat acccagtaca ggaagaattc tcatcggcaa caccgatata
     4201 acagatttac ctccctataa aagacaagtt aatactattt ttcagaatta ttctcttttt
     4261 ccacatatga cggtttttga taatgttgcc tttggattgc gcattcgtaa attccctaaa
     4321 aatgaaatta acgacagagt aaaagaaatg ctcaccttag ttaaaatgga agatcaggcg
     4381 aacaaatatc ccgaccagat atcgggtgga caaaaacagc ggatagccat tgctcgcgct
     4441 ttaataaata aacctaaagt gctattgctg gatgaacctt tggctgctct tgatctaaaa
     4501 ctccgccaac atatgctaat tgaactaatg aatattcatg atgctgtagg aataaccttt
     4561 atctatgtaa ctcacgatca gagtgaggca atgagtattt cagaccgcgt tgcggtaatg
     4621 aattatggtc atataattca atcggggcca cctgatgata tttatgaacg gcctaccagt
     4681 gttttttctg catactttat cggagctacc aatctgataa cgggagaagt aattgggatt
     4741 gaagatgatt atgtgcaaat cagatgtccc gatggcactg gcaatagctt tgaaatagac
     4801 agtacattcc actttcaacc cgctatagga caagaattaa caatatctct gcgtccggaa
     4861 aaaattgcta tttctcgtaa taaacctcgc taccaagaag atataaatat attacgcgga
     4921 gttattgaag atattttata cttgggttcg catactcaat atattgttag agttggagac
     4981 ctaagaatta gagtattcag ccaacataaa agagtttatt ttgatgatac agcactagac
     5041 tgggaagaac ctgtatggct attttggcat gatactgata cttggcttat cgatgaaatt
     5101 cccgaaaatg ttttaaccgc tgatgagctt tccgagggtg gaattaacca ttttcagcgt
     5161 tcattgaagc gtcctacagc acaatgaaaa ttatccctga aaataatcct aattatgctc
     5221 tacaattagc taccgagcga aaaaagaact tcacctcttg gcttttaaca gctccggctt
     5281 tgctatggct gattttcttc tttttgattc cctatcttat tgtaataata tacagttttt
     5341 taactgcaga tatctatgat gtaaagtttg aatttacttt ggaagcatat cgtcagtgtt
     5401 taacacctgc ctatcttcat acactttttc tttctttccg gctggcttta ttaacaaccc
     5461 tattatgcct tttattggga tttccggttg cttattatat tgcgcgagca aaggacaaag
     5521 taaaaaacac tctgttaatt tttattatta tccctttctg gactaacctg attatccgca
     5581 ttttttcctg gcgtatcttt cttgcctata atggcgtctt aaacaatata ttattaaatt
     5641 tagggcttat ctcacagcca ctggaaatta tgagaacaga tattgcagtt attttagtga
     5701 tggtatatgt atatttaccc tatatgattt taccgctata cagcgtgtta gaaaaacttg
     5761 attggactct tctgaatgcc gcgatggatt tgggagctaa tgaaattata tcttttttta
     5821 aaataacctt gcctttagcc aggggcggca tctttgccgg tggcttactt gtttttatcc
     5881 cagctttagg ttcatattta atacctcagc tggttggaaa tcaaaaatcg ctttatttgg
     5941 gacaggttat cacctataaa gttaaaaaca tacctcgcaa ttggccaatg gcttctgctt
     6001 tatcacttat tctactgttg attgtagccc tatttctaat tatgttctat gtcctttatt
     6061 ctcacagcaa atctcggagg cgagtgtgaa acgcttctct ttaactaagt tatttaaagg
     6121 tttgaattta acttccttca ctttagtaat ggtgtttctc tatattccca tcttgatctt
     6181 gattattttc agtttcaacg atgctaaaat cattactggt tggaagggct ttactttaaa
     6241 atattatgct cagttgctga aaaatgaaac tatccaaatt gcttttagaa acacgatgct
     6301 gatagcttgt ctttccacct taatatccac tattttagga gtgctaaccg ctttgggatt
     6361 ggagaataaa aagttcagcg gaagtaaatt cgtttccatt ctaatctata ttcctttagt
     6421 tatacctgat attttgatgg gggtctcttt agctttactg tttaatttca caaaagtaaa
     6481 cacaggaatg ctgacagttt taattgctca tatcactttt tgcatttctt acacaattat
     6541 tgtaattcgc agtcgccttg atgattttga ttattcttta gtagaagcag caatggattt
     6601 gggagcaaag ccctcaacca tcttctggaa aataaaaata ccattaatct tgccgggaat
     6661 tattgctgct gcccttctag cttttactct ttccatagat gattttatta tcactttttt
     6721 cacttctgga agaggtttcg atactttacc aatttatgtg gaaggcgcta tccgccgggg
     6781 aacaattact accatcaatt ccctatccac tattatgatc ggtttaacct tactgctggt
     6841 agttactaca aaacgcatcc gtaaaatcgt tatttcctct ctataatctc cttttttaac
     6901 acagtattct ctatctataa gcgtttggtt tgataatgta gttttctgtt acttgtattt
     6961 ccgaaatagg acacagtatt cattataatc tatatgggtt cctgcttaca aaggaaataa
     7021 caatgagaga aaagaatgtc agctaaagga agtaatattt cgggaatttc gtttgacata
     7081 aacatcaggt aaacatttat ggcaatatga atgagattaa aatagacaaa aattcgccaa
     7141 atcaatttga taccttacct aagggttggg ttttttatct gctttcaggt ttgaatgaag
     7201 aactatatgc tggttattct gccaatctga aggttaagtt aaattatctt taccataaag
     7261 ctatggaagg tggcatctat caagatatgt ggaattctgt ggaaaaattg aattgggtta
     7321 cctacgatag ggctatctct gcgttaattc actgtaaatg ctatctagca gaaaatatgc
     7381 cactatatca atatcaaatt cgtccttggg aagaatatgt atacctatcg ttaaattcac
     7441 atcgctatcc ttttatcgca attcaggaac atactagtga cgattggttg tatttaggac
     7501 cttttcgtaa tcgctttttc ctggttgatg ttttggatat tttttcgcgt atcttaaaat
     7561 taccagcttg tgaaacaggt gaatatcctt gtgacaaatg ggaacagaat ctttgccagg
     7621 gttggtgttt ggctctggca gataatgcaa aaataaatcc tgagtattcc ttggacaaat
     7681 tagaatccct tttaaaagag acatttttgg tccctgaaaa tggtgttttg gaacttgttc
     7741 aacaggaaag agacagatat tttaataacc tggaatttac aaaagcatca ttgctaaatg
     7801 acgagataga actgctaagc aaataccgca attggttgaa atttttaatt gctgccaaac
     7861 atttgaatta caatagtgaa gaccttgaaa tacaaaaagg taagcttact ttttgcaact
     7921 ttcaggggcg aaaatatcat tttcctattg acaatacaga gtttagaatt aacgagacac
     7981 ttgctcttaa tctggatacc gtagatgaaa gtaaaattat ctacgatttc ttaaaactga
     8041 cataaggagt ttttttatgc acaacgaaat aagcaaatta gctatagtaa aagaaagcca
     8101 tcaaggcaaa gtacgcgata tatacgattt aggagaaaac cttttaattg taagtacgga
     8161 tcgaatctcc gcttttgatg tagtttttcc agatcctatc cccgataaag gaaaaattct
     8221 gaatgctatt tctgctcatt tttttaagac tacttctaat atagttccaa atcattttat
     8281 tactgataat gtttctgatt accctaatga actacagaag tttaaagaat atttagacgg
     8341 acgcagtatg ttagtaaaaa aaacacgagt aattcctttt gagtgtattg tgcgtggata
     8401 tattagtggt tctgcctgga aagaatataa aaaaactaat acaattggag ggatgctgat
     8461 tgcggaagat atgcaggaaa gccaaaaatt cactcacccc ctcttcactc ccagtaccaa
     8521 agtagaagat ggtcacgatg agaatatcag ctacagggaa tttatgaacc gaatggatga
     8581 attattggct gtttatataa aggataaatc actggagttg tattctgacg ctcacgattt
     8641 actcctgaaa aaaggagtga ttttagctga tactaaattt gaatatggaa ccatcggctc
     8701 gcaagtgcta ttgattgatg aagttttcac tccagattct tcgcgtcttt gggctttgga
     8761 aaattacgaa ataggaaaaa gtcccgatag cttcgataaa caatttatcc gagactactt
     8821 ggaaaatagc ggatggaata aacaacctcc tgctccatct cttcctacag aaataattga
     8881 aaaaacccgc caaaaatatg taactatcct aaaaattatc acaggaaaag agctgtaaca
     8941 atgaaaaaaa tcatctgtat cgtagacgac gaagaagaat tagttgaaaa cctgaaaata
     9001 gaactggaac acttaaaacc ggaatggaat atccttactt ttagcgaagg aatgaaagca
     9061 atccaggaaa tcgtgaaagg gaccgtagat ctcgtagtta cagatattgc tatgcccgat
     9121 atggatggct acgagctgtt ctggagaatt aaggattacg atgaaaatat tccggtgatt
     9181 atgatgaccg gctttggtta cgaccccaat catgttttag tacgcgccaa agttgacggc
     9241 ttgaaagatg ttctctataa acccttcgaa acagaaaaac tggtggaact tattgaatcc
     9301 cacttaaaat aaatggatct caatacattc atccaaaccc atttactaaa gcgctctcct
     9361 ttgtcttatg ctctatatcc ttcgggagta ctctatggct atatccaaaa acaatgcaga
     9421 acatattctc gccgaagagc ttatcgcgca ccttgcaaaa ttattagtat tggcaatatc
     9481 attagtggtg gttcgggaaa aactccactc acaatttctt tggctaattt attgataaat
     9541 aaagggctaa aagtagctgt gtcccatcgt ggctataaaa gtgcttttga aaatgaacct
     9601 catattatta acgccgattc tggtatgctc tacgatattc ataaaacagg tgacgaggca
     9661 tatttgatag cctctgccct acttaaagtt cctgtggcag ttggtagaaa acgggtgttg
     9721 gctgttaaat tactcttaga aaaacatcct tcgctggatg tgattatttt agacgatgcc
     9781 ctacaacata attatatcca tagagactta gatataatca gttttgatgc ttctattgga
     9841 ctgggcaacg gctttgtgat tccagcagga tatttaagag aaccactttc caatatccct
     9901 caaaacagtt taataataat taaccgcaaa aataaagcac aaacagaaac cctttggcta
     9961 aataaaatta accaattgaa aataccaatc taccatagct ctaatactcc aaaatgcttt
    10021 ttggacatta ccggaaatgt ttatccgctc gattatctta aagaaaaacg aattgtgctt
    10081 gtttccggaa ttgccaatcc agcttccttt gcagattctg ttagttctgt gggactatct
    10141 tatgtaaagc actatgcttg tatagatcac tgtgatttta aacagccatc cctaatagaa
    10201 aacatcttgg aactaaaccc ggattatatt ctctgcaccc aaaaagatat tatgaaactg
    10261 gctaaatatg aatccttgca aaatcgctta cttgcgctaa agctggaata tagctttgac
    10321 caagaagatg aatttcttaa ccaggttcta aattttatta agtaactaat cggtaaattg
    10381 ggaaaactta ctacatcatt ctcattacag ttagataata ttggcacact attaaccttg
    10441 ttcagttaat gaggtagcca aacaaattac acaataaaat tattgtacct ctccaattga
    10501 cactccctat ctttttatgc caaccttttg atatactacc tctcccaata tctagtggat
    10561 tgctcagcat aaataataaa agcatattaa gatcgattat tcgaagctgt tgaaatctat
    10621 taagtccaat aatcttaata agttataaat aactgttttg ccctaagcct tactttcgct
    10681 tgtaagttaa cgatatacaa tttgtcctcc acccaatctc ctctcctcct taactatggg
    10741 ttgaagagga gaggaggagg agatgaggag gggttgtttg ttcgaatcat cttgaaaaca
    10801 gtggacttaa tttagggaaa aaggaatgcg ttaaaaaatc ctaccttaaa tcctcgtttc
    10861 gaaaactaaa tctgcgcaat ctgcgtaatc tgcgtgatct gcgtgaaaaa tcttcggtga
    10921 tttcggtgat ttcggtggct aataaataaa tcctctaaat cctaaaatcc ttaaatcctt
    10981 gtttctaaaa tccaatcctt aaatcctcgt ttctaaaatc caatccttaa atcctcgatt
    11041 cgaaaactaa atctgcttaa tctgcgcaat ctgcgtgctc acattttttc ggtgatttcg
    11101 gtggcttcgg tggcttataa ataaatcctc taaatcctaa aatccttaaa tcctcgtttc
    11161 taaaatccaa tccttaaatc cttgtttcca aaatccttgt ttattataaa ctattttaat
    11221 tctatcgaat tcctaaaatt gatagataaa aactcgttca acaagaataa ataaggcatt
    11281 cccacaaaaa agtgatgtgg acaagttata acacactgtt gtatag
//
LOCUS       DAOSTH010000050        11213 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_5718_length_11213_cov_12.470783, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000050 DAOSTH010000000
VERSION     DAOSTH010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11213)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11213)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11213
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5718_length_11213_cov_12.470783"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            985..2451
                     /locus_tag="PLB65_06255"
     CDS             985..2451
                     /locus_tag="PLB65_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78166.1"
                     /translation="MKQILTVVLMSITALLMAQYEIPFGNIRHSSIDANGNIHLRWED
                     LSGGTLNPEFFYSANDAPWQSSPIQDISTGEKEALAPYTLGQKLRYRLHYSIEEMGES
                     IAMMQSAYWDNDTFPPQLDKMAYIATDAEGDSVTVYSQNLDLRESYTAISSDKIYFSM
                     QNLSGNFPIMNSLTSYNVYLGVLGNISSLMDSVGYAMIYSFNIPGVISSGLYKVGYDS
                     ATQMPVFTRLGNVQSQVSGGALNLACNISDLTADPDFGSLPDALLMLGLTLKIDIDLA
                     NMEPQIGLGDYSTPAVVIFQDNFYQVNQNTLPVCTFNSYDTITRLLQIDYLDAEADFP
                     LLAEAELPDGTILQFVPTGVDYSLAVTYTLQLPSAEVDSLTWRFSDNGFDIVQGVVHL
                     VANHDENLVPTNISCIMPNPIYSFPVNINLKGLCNKPIFIDIYNIKGQKVAELNQPPA
                     INGELTISLAPKQLCSGVYFMKVKNGPYVLKQKFMVAK"
     gene            2460..3470
                     /gene="obgE"
                     /locus_tag="PLB65_06260"
     CDS             2460..3470
                     /gene="obgE"
                     /locus_tag="PLB65_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOQ78167.1"
                     /translation="MFIDYAKIRIKAGNGGDGIVSFRREKYVPRGGPDGGDGGKGGDV
                     LAIGDTNLNTLLDYRYHKIFKAGNGKPGAGAKKSGAKGEDCILRLPLGTEIYVIEEEK
                     RYKLADITEAGEYVILSKGGYGGKGNCNFATPTNQAPRTATLGIKTEELELELVLKLM
                     ADVGLVGFPNAGKSTLLSVLSAAKPKIADYEFTTLEPMLGVVYVNDYQSFVLADIPGI
                     IEGAHSGKGLGDQFLRHIQRTHLLLFLIDIDAEDPVEAYNTLKKELYLYDSFMDKKPH
                     LIVISKTDTVPPEELDQRIAAIKEAFKKHHSEDIIAISAVSNYGLDPLKYKIYDLLEQ
                     SR"
     gene            3471..4559
                     /gene="dprA"
                     /locus_tag="PLB65_06265"
     CDS             3471..4559
                     /gene="dprA"
                     /locus_tag="PLB65_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOQ78168.1"
                     /translation="MEKLKAWLCLKSAPLLGPKGVLDILQHYPDPQEFVGNTAHNIYR
                     EERLKPETINHLSQFILPENNNKILDLMAKYQIHYLGLTDPDYPACLKDICDPPLILY
                     YRGNLSETLNELCVGVVGTRKPTSYGVEMTRKILSPLCEKGVNIISGLAMGIDTIAHQ
                     TALKAKTKTIAVFASGLETIYPPMNKALAEHIIDKGALVSEYEPGSKLERWNFPARNR
                     IISALSQAIFVVEGSLNSGAMLTAKFAQQQNKPLYALPGNINNRNAQGPNLLIRQGAT
                     LVSSADDLITDFGLSSSVKEQLELIPELSAEEQSLFDLLSEAQRDITFDEFMLKTGLG
                     FGKLSTLLLNLELKGVIAKSSGNSYIKL"
     gene            complement(4833..5786)
                     /locus_tag="PLB65_06270"
     CDS             complement(4833..5786)
                     /locus_tag="PLB65_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78169.1"
                     /translation="MRRILLILAISLYWLCGFCLTIEVSDYGYTEGKIRNYNEGPWNS
                     SGTDLSEGTNKLWSFNMPSAGYVNNIFHCVQNVSGFPNANMSADFEQNILDYYSSGTQ
                     YYQNTGSDILSIGYTASPNFVWNPPVPMGLPHYLGKTWSGTHNWAYGTYVVSGKVIAE
                     GQVSTPLGSYPALLVRYYYQTNVISYYYYQWETKEYGIIAYTLTVNNGMLYVLNQADP
                     NVANEDNVAQTHSLKAVLSPNPFASDLSLILEAKNSSPVCISIYDLKGRLIAKSDHIL
                     KAGAITQLDFSEVFVNQTAGLYFVSINYGSGKLVRKVTKLP"
     assembly_gap    5891..5990
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(6317..6919)
                     /gene="nadD"
                     /locus_tag="PLB65_06275"
     CDS             complement(6317..6919)
                     /gene="nadD"
                     /locus_tag="PLB65_06275"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HOQ78170.1"
                     /translation="MQGVVAVLGGTFDPIHLGHLQIATEIIKQKAADSVLFVPSGHHH
                     FKKNTIILPYAKRYSLVEKAIKNNPKFDISDADKEGSGYTADLMKKLFCLYPQVNFRF
                     VIGSDNLPQLNKWYDYAYLVENLHFFIFPRPGYPLLPQVINQIKATILDISPCPVSAT
                     EIRQRIFNGESIKGMVPEELEEEIVYLYQNKDNTKAKNQN"
     gene            complement(6921..7655)
                     /gene="bamD"
                     /locus_tag="PLB65_06280"
     CDS             complement(6921..7655)
                     /gene="bamD"
                     /locus_tag="PLB65_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOQ78171.1"
                     /translation="MRKYLSLIILIVICLFSCSSNKTQLTTEAKLKNADELYAKGKYS
                     RAAVIYEEISFERKSAATAYATMKVADCYYNMHKFSEARLRYEQFISSFPDHEKVADA
                     YFRIGECFFEESLPPHYDQTETIKCIEAFKTFIDRYPSDPRYGEAIDYIQKCQYKLLE
                     KDYLTGYLYYKMKDYSSALMYFDEIIELGNINDLELKSLYYSAKLHLHQKNYEKAKAN
                     YDKLLSRYPNSKEAEKLKRKFEKSVL"
     gene            complement(7672..8247)
                     /locus_tag="PLB65_06285"
     CDS             complement(7672..8247)
                     /locus_tag="PLB65_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78172.1"
                     /translation="MPKYLLPLCFFVLCANFLLSQESLALDSIATSIAEEIQIGQPIF
                     LDINCGDWNPVLTEKLSSKLLEAGADLRYNLSDGKGAITFTEEGKAIDLSAFGLSEAL
                     LIQVNLNILWKEESKSSFFSYKTQRYPVHIFETRQIELPSQQLKKISTYSLPLPENKT
                     SGTSSLHRHWFEPIVAGAAIGSLVFLLWNFD"
     gene            complement(8251..9663)
                     /locus_tag="PLB65_06290"
     CDS             complement(8251..9663)
                     /locus_tag="PLB65_06290"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOQ78173.1"
                     /translation="MKNTKPEKEALAYQRKNFWKEAPLEEQKKAMEYAEPYINFLNQA
                     KTERETVAYTLDLLLKNKFTPLSKKGSKKVYSIFRNKTMAVAVIGTDPVSNGFNMVAA
                     HVDSPRVDLKQNPLYEDSNSAMGCMRTHYYGGIKKYQWVSTPLALHGVIIKNDGSTLN
                     ICLGEKEDEPVFIIPDLLPHLAHKEQYTKNLGDAIDASKMNLVFSGMQEPNSDEKEAI
                     KAYALKLLNEKYGITEQDFLSAELQLVPAHKARNCGFDNSMIVGYGQDDRISAYNALM
                     ALINNIPEKPKRTMVVYFSDKEEVGSQGSTGAHSIFIRDFISDLLAHNQEDNGSANLR
                     KTFMNSQILSADVSAAIDPNYPNVHERQNAILFNYGIGINKFTGSGGKYGCNDANAEF
                     LAKVLRIFNEAGIYWQLGELGKVDEGGGGTIAYILSNLGAEVLDCGVGLMGMHSLYEL
                     SSKADLYSTYLAYKAFLKAE"
     gene            complement(9684..10163)
                     /gene="greA"
                     /locus_tag="PLB65_06295"
     CDS             complement(9684..10163)
                     /gene="greA"
                     /locus_tag="PLB65_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOQ78174.1"
                     /translation="MDLSQYITKTGMQRLQKRINELLTERPEVIKAIAIAREFGDLSE
                     NAEYKAARERQRAIDNEIDYLRCRSAKLQVIDTSEFPKDIVRFGSYCVAEDISNNECI
                     CYHLVGADELNYYDEEGIQEVSVVSPIGRALLSKKIGEIAVVHAPMGERHLKVIEIK"
     gene            complement(10171..10980)
                     /locus_tag="PLB65_06300"
     CDS             complement(10171..10980)
                     /locus_tag="PLB65_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78175.1"
                     /translation="MLTIDWKERLNMDTADFLKNKLPKGDYDFEIIFIAYPERVNGKI
                     PAEVISQVANNIVQQLGKKHELYLPFYNALWAKKGDYGKQAFAQILSKLLHRKPSVYM
                     PVLEGAISTADEAEINTLLDKVMLPLIRKYPDKYLDKVFEFGNSQNLDLQSAALNLLI
                     KLIKKREDLIPQVVNYFIHQWLYPLGEAIAVHIAMLKTVAKIDPEYYVNIWREYGSSR
                     DPQIVELLCASITDYYPELENSIEIWTKSGNARLKKAATSAQRILMKKKRS"
BASE COUNT         3353 a         2100 c         2139 g         3521 t
ORIGIN      
        1 caaaaatcta acatagggaa actgacttgc gagaatgaac ggtttattga ggatacagaa
       61 tatgtaaaaa tatatgcact gctgcctcca agtacgagta tttttgtttt ttattttggc
      121 aatacaattt agatagtttg gaatcttggg caattcatcg atctttttca tcttcataaa
      181 ctccttatct gtcctatgtt attacctctt ttgccgaaaa tattctttgt ttcctatagt
      241 cacaatttct actgttgcca tcaccataat attgtcaaga actttttgac aattatgcct
      301 ttgctgccct aaaccttgtt ctaacggtga tttcttttaa gcttggttcc aaaggctggt
      361 aaaattccct tttccttgaa ggtctgaaag ataattgtta tatcctttcc ctgtgctcaa
      421 tatagaatat atgaacatcg aatctcttat ataaacctgg ttttaggttt gcggcaatct
      481 ccacaaaatc cacctgataa aaatacagtg aaacaatatt gtctccctgt tgaggataca
      541 gctatttttt ttacttactt ggatttctgc ttacccagaa atgacaggga tattttatct
      601 aatagctatt taacttaggc acttactaaa ggtgaattag gcagatcatt tctccgctct
      661 gccttatttg aaactaaaaa aacacagtag ttgttttatg cttaaggatt tataattatt
      721 ctgtccggga gctcaaaata ggcagatgct agcaagctac acaaatcata cctgccttgg
      781 gattgttgct ttgcagaaaa agcttgacat ccacagggct ttagagtatt atattataaa
      841 tgatttatct tgtttagata cattggggat aaatgcttcc gtgcctttgc aaaaaagaat
      901 ttggtaagca gttaggttcc caaaggaaat tgggtgtaac aagaaagaat tgatatttgt
      961 aaggatcaaa aaagcgagaa agcaatgaaa cagattttaa cggtagtttt aatgagcata
     1021 accgcattac ttatggctca atacgaaatt ccgtttggca acattcggca tagttccata
     1081 gatgcaaatg gaaatattca tttgcgttgg gaggatttaa gcggagggac attaaatccg
     1141 gagttttttt atagtgccaa tgatgcacct tggcaaagtt cccctattca agatatttcc
     1201 acaggagaaa aagaagcgtt ggcaccttat actttaggtc aaaaattgcg ataccgtctt
     1261 cattacagca ttgaagagat gggtgaaagc attgcaatga tgcaatctgc ctattgggat
     1321 aatgatactt ttcctccgca attagataaa atggcttaca tagctacgga tgcggaagga
     1381 gatagtgtaa cagtatattc tcaaaatctg gatttacggg agtcctatac agcgatcagt
     1441 tcagataaaa tatatttttc tatgcagaac ctcagcggga attttccgat aatgaattcc
     1501 ttaaccagct ataatgtata cctgggagtt ttgggcaata ttagttcgct gatggatagt
     1561 gtaggctatg caatgatcta tagttttaat attcccggag taatttccag cggattgtat
     1621 aaagtgggtt atgattcagc aactcagatg cctgttttta cgcgtctggg caatgtgcaa
     1681 agtcaggttt ctggaggggc attaaatctt gcttgcaaca tcagtgattt aactgccgat
     1741 ccggatttcg gttctttacc ggacgcttta ttgatgttgg ggcttacgct gaagatagat
     1801 attgacctgg caaatatgga accacaaata gggctgggcg attatagcac tcctgccgta
     1861 gttatttttc aggataattt ctatcaggtg aaccaaaata ctcttcccgt ttgcactttt
     1921 aacagttacg atactatcac tcgtctttta cagatagatt atctggatgc ggaagctgat
     1981 tttcctttgt tggcagaagc agaattaccc gatggaacaa ttttacaatt tgtacctaca
     2041 ggtgtggatt attctttggc cgttacctac actctgcaat taccgagtgc agaagttgat
     2101 tccctcactt ggaggtttag cgataacggt tttgatatag ttcagggagt agttcattta
     2161 gttgctaatc acgatgaaaa tcttgttccc acgaatatat cctgcattat gcccaatccg
     2221 atatattcct ttcctgtgaa cataaacctg aagggcttgt gtaataaacc gatatttata
     2281 gatatttata acataaaagg acaaaaagtg gcagaactaa atcaacctcc agccataaat
     2341 ggcgaattga ctataagttt agcccctaaa cagctttgtt caggagtata ttttatgaaa
     2401 gtgaaaaatg gaccttatgt ccttaaacaa aaatttatgg tggcaaagta acagcagata
     2461 tgtttattga ttacgcaaag ataagaatta aagccggaaa cgggggtgac ggcatagtta
     2521 gtttccgacg cgaaaaatat gttcccagag gtggacctga tggcggcgat ggaggaaagg
     2581 gaggagatgt tcttgctatt ggagatacta atttaaatac tcttctagac tatcgatatc
     2641 acaaaatctt caaagctgga aacggaaaac cgggtgcagg agccaaaaaa agtggcgcta
     2701 agggagagga ttgtattttg cgtcttccct tgggaaccga aatatatgta attgaagaag
     2761 aaaaacgcta taaattagca gacatcacag aagcgggaga atatgttatt ttatcgaagg
     2821 gtggttatgg aggcaaagga aattgcaatt ttgccacgcc tacaaatcaa gctcccagaa
     2881 ctgctacttt aggcataaaa accgaagaat tggaactgga actggttttg aaactgatgg
     2941 cagatgtggg tttagtgggt ttccctaatg ccggtaaatc aactctttta agtgttttat
     3001 ccgcggcaaa acccaaaata gcggattatg aatttactac tttggaacca atgctgggag
     3061 ttgtgtatgt taatgattat caaagttttg tgctggcaga tattccgggc ataattgaag
     3121 gtgcacattc tggtaaggga ttgggagatc agttcttgcg acatattcag cggactcatc
     3181 ttctgctgtt tctaattgat attgacgctg aggatcccgt tgaggcatat aatactttga
     3241 aaaaagaact gtatttatat gattccttta tggataaaaa gccgcattta attgttatta
     3301 gtaaaaccga tactgttccg cctgaggaat tagaccaacg aatcgctgct ataaaagagg
     3361 cttttaaaaa acaccatagt gaggatatta ttgctatctc tgcagttagc aattatggtt
     3421 tagacccgct taaatataaa atctacgacc tcttagagca gagccgctaa atggaaaaat
     3481 tgaaggcctg gctttgcctt aaatcagctc cccttttagg accaaaaggt gttctggata
     3541 ttttacagca ctatcctgat ccacaagagt ttgtgggaaa cactgctcat aatatctatc
     3601 gggaggaaag acttaaacct gaaactataa accacttaag ccaatttatt cttccggaaa
     3661 acaacaataa gatcttggac ttaatggcta aatatcagat acattatctg ggtttaactg
     3721 atcctgacta tcctgcctgt ttaaaggata tttgtgatcc gcctttgatt ttgtattaca
     3781 gaggaaacct ttcagaaacg ctaaatgagc tttgtgtagg agtggttgga accagaaaac
     3841 ctacttccta cggagtggaa atgactcgga aaattctctc tccgctctgt gaaaaagggg
     3901 tgaatattat cagcggtctg gcaatgggaa ttgatacaat tgcccatcag acagctctga
     3961 aagcaaaaac taaaactatt gccgtttttg cttccggttt ggaaactatc tatccaccaa
     4021 tgaataaagc ccttgccgag cacattatag ataagggagc attagttagc gaatatgaac
     4081 ccggcagcaa attggaacgc tggaattttc ctgcgcgcaa tagaattatc agtgcccttt
     4141 cccaagcaat ttttgttgtg gaaggttccc tcaatagcgg cgctatgctt actgccaaat
     4201 ttgctcagca acaaaataaa ccgctgtatg ccttgccggg aaacattaat aaccgcaatg
     4261 cacagggacc taatttacta atccgacagg gagctacttt agtaagttcc gcagatgatt
     4321 taatcactga ttttgggcta agctcctctg taaaagaaca gctggaattg attccagagc
     4381 tttctgctga agaacaatca ctatttgacc ttctttcaga agcacaacga gacatcactt
     4441 ttgatgaatt tatgctaaaa accggactgg gcttcggaaa actttccacc ttgctattga
     4501 atttggagct gaaaggagtg atagcaaaaa gtagcggaaa tagctatatc aaattgtgag
     4561 atagttactt gtaacataga aagaaaggaa gcaagaggta tattttattg agatagttac
     4621 ttgtaacata gaaagacaag gaagcaagag gttattttta tattcactgg taaactaaaa
     4681 acccagaaac cctgtcccaa tcttctcatc agaattgaat aacaataaga cccttcctgt
     4741 tatccctcca attttagttg ataaatatag gtagctgatg ggaatggata ttaaggaggg
     4801 ataccttttt cctttacccc ccaagaaatg gcttacggta atttagtcac cttgcgaacc
     4861 agtttcccgc ttccatagtt aatagagaca aaatacaatc ccgccgtttg attgacaaag
     4921 acctcgctga agtcaagctg tgttatcgct ccggctttca ggatgtgatc acttttggct
     4981 atcaatctcc cttttaggtc gtagatactt atgcaaacag ggctggagtt ctttgcttcc
     5041 agaatcaggg agagatcact ggcaaaagga ttggggctaa gaaccgcttt cagagaatgg
     5101 gtttgtgcca cattatcttc attggcaaca ttgggatctg cttggttcag aacatagagc
     5161 ataccattat tcacagtgag ggtgtaggca attattccat attctttggt ttcccattga
     5221 tagtagtaat agctgatcac atttgtttga taatagtagc gaaccagcaa agcaggataa
     5281 cttcccagtg gtgtgctaac ttgaccttcg gctatcactt tgccactaac tacataggtt
     5341 ccataagccc aattatgcgt ccccgaccag gttttgccca aataatgtgg taagcccata
     5401 ggaaccggtg gattccaaac aaaattcgga gaagcggtgt aaccaatgct gagaatatcg
     5461 ctaccggtat tttggtagta ttgagttcca ctgctgtaat aatccagtat gttttgctca
     5521 aaatcggcag acatattggc attggggaag ccgcttacat tctgcacaca gtggaagatg
     5581 ttattgacat atccggcaga aggcatatta aagctccaaa gcttgtttgt gccttcagag
     5641 agatctgttc cagaagaatt ccaaggtcct tcgttgtagt ttctgatctt tccttctgtg
     5701 tatccataat cgctaacttc gattgtaagg caaaaaccac atagccagta tagcgagatc
     5761 gccaatatca atagtatcct gcgcattatt agattcctta ttcctttttt tttagtaaat
     5821 tatgagggcg ttttcttcta ttagctttaa taataacgcc tgccagattg tgtcaacttc
     5881 tctttctgct nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aacataacct
     6001 cccaattcaa tgaatgaagt tatgagcgtt ctgattgagt gctttgaata ccttgatgaa
     6061 aacaagggga aatggtctca aagttcactt atgtcaaata gcaaactttg gttattgaaa
     6121 gcgaaatggt atgatctcaa cagcactctt ggcagatgca ttgattttat gtagccaatg
     6181 ggcaagaata gtaagtggga tacttttttc cttttccact caagatacta ataaggaaag
     6241 tgttaggaga gtaatagtta gagtgagcaa tcgctatttt ggtgattaac tttttatttt
     6301 atataacttt ctaagtttaa ttttgatttt tagctttcgt attgtcttta ttttgataga
     6361 gataaacaat ttcctcttcc agttcttccg gcaccattcc tttgatgctt tctccgttaa
     6421 aaattcgctg tctgatttcc gttgcggaaa caggacaggg agaaatatct aaaatggtag
     6481 ctttaatctg gttaataact tggggcaaaa gcggataacc cgggcgggga aagataaaaa
     6541 aatgcaaatt ttcaaccaga taggcgtaat cgtaccattt attcagctgg ggaagattat
     6601 cggaaccgat aacaaatctg aaattcactt gcggatacag gcaaaacagt tttttcatta
     6661 aatcggcagt atatccgctt ccttctttat cggcatcgga gatatcaaat ttaggattat
     6721 ttttaatagc tttttccaca agggaatagc gttttgcata ggggagaatg atggtatttt
     6781 ttttaaaatg gtgatgtcca ctggggacaa aaagaacgct atcggcagct ttttgcttta
     6841 tgatttcggt tgctatctgc aaatgtccca aatggatagg gtcaaaagtt ccacctaaaa
     6901 cagcaactac accctgcact ttataatacc gatttttcga attttctttt tagtttttct
     6961 gcctctttgg aattaggata acggcttaat aatttgtcgt agttagcctt ggctttttca
     7021 tagttttttt gatgcagatg caatttggca gaataatata gcgattttag ctctaagtcg
     7081 ttaatattac ccaattcaat gatttcatca aaatacatta gggcgctgct atagtccttc
     7141 attttgtaat ataggtaccc ggttagataa tccttttcca aaagcttata ctggcatttt
     7201 tggatataat caatagcttc accatagcgc ggatcactcg gatagcgatc tataaaggtt
     7261 ttgaatgctt cgatgcattt aattgtttcg gtttgatcgt aatgcggagg taatgattct
     7321 tcaaaaaagc attctccaat gcggaaataa gcatcagcaa ccttttcgtg gtctggaaaa
     7381 gaactgataa attgttcata cctgaggcgt gcttcgctaa atttatgcat attataatag
     7441 caatcggcaa ctttcatagt ggcatatgcg gttgctgcag atttacgctc aaaagaaatt
     7501 tcttcataga taactgctgc tcgggaatat tttcctttgg cataaagctc atcggcattt
     7561 tttaattttg cttcggtagt aagctgggtt ttattgctgc tgcaggaaaa caggcagata
     7621 acaataagta taattaatga tagatattta cgcaagaaag tatccttttt tttaatcaaa
     7681 attccaaagg agaaaaacta atgaaccaat agctgctcct gcaactatgg gctcaaacca
     7741 atgcctatgc aaagatgaag ttccggatgt tttgttttcc ggaagaggta aactgtaagt
     7801 agatattttt ttcagctgtt gcgaaggtag ttcaatttgc ctggtttcaa aaatgtgaac
     7861 aggataacgc tgtgttttat aggaaaaaaa actgcttttg ctttcttctt tccagaggat
     7921 attcagattc acttgaatta gtaatgcttc acttagtcca aaagctgaaa ggtctatagc
     7981 ttttccctct tcggtaaaag taatggcacc tttaccatcg gatagattat aacgcaggtc
     8041 tgctcctgct tctaaaagtt tggaacttaa tttttcagtt aaaacggggt tccagtcccc
     8101 acaattaatg tccaaaaaaa tcggctgccc tatctgaatt tcttcagcta tgcttgttgc
     8161 tatactatcc aatgctaagg actcttgaga taacaagaaa ttagcacata gcacaaaaaa
     8221 acagaggggc agcagatatt ttggcatagc ttattcagct tttaaaaatg ctttataagc
     8281 cagataagtg gagtataaat cggctttaga actaagttca taaagggaat gcatacccat
     8341 taaaccaact ccacaatcta aaacttcggc tcccaagttg gaaagtatgt aagcaattgt
     8401 gccaccgcca ccttcatcaa ctttacccaa ttctcctaat tgccaataaa ttccagcttc
     8461 attaaatatt ctgagaactt tggctaaaaa ttcggcatta gcgtcgttgc aaccatattt
     8521 tccaccgcta cccgtaaatt tgttaattcc aattccataa ttgaaaagaa tggcattctg
     8581 tctttcatgc acattgggat aatttgggtc aattgctgca gacacatcag cacttaatat
     8641 ctggctgttc ataaaggttt tacgcaaatt ggcactacca ttatcttctt ggttatgagc
     8701 aagcagatcg gatataaaat cgcgaataaa aatactgtga gcaccagtac taccctgact
     8761 gccaacttct tctttatcgg agaaataaac taccattgtg cgttttggtt tttcaggaat
     8821 gttgtttatt aaagccatta aagcattata ggcactaatg cggtcatcct gtccataccc
     8881 aacaatcata gaattatcaa agccacaatt acgagcttta tgagctggca ctaattgcaa
     8941 ttcagcgctt aaaaaatctt gttcagtgat gccgtatttt tcgttaagca gctttaaggc
     9001 ataagcttta attgcttctt tttcatctga attaggttcc tgcattccgg aaaaaaccag
     9061 gttcattttg ctggcatcaa tagcatcccc taagttctta gtatattgtt ctttatgagc
     9121 taaatgcggt aaaagatcgg ggattataaa aactggctcg tcttcctttt caccaaggca
     9181 aatgttcaat gtggaaccat catttttaat gataactccg tgtaaagcca agggagtaga
     9241 tacccactga tattttttta taccaccata ataatgagtg cgcatacaac ccattgcact
     9301 gttgctgtct tcatatagag gattttgttt aagatcaact ctgggtgaat ctacatgagc
     9361 agctaccata ttgaatccat tagaaacagg atcagttcca ataacggcta cagccatcgt
     9421 tttgttacga aagatgctat aaactttttt gctacctttt ttgcttaaag gagtaaattt
     9481 attcttaagc agtaaatcta aagtgtaggc aacagtttcg cgctctgttt ttgcctgatt
     9541 gagaaagttt atgtatggct ctgcatattc catagctttc ttttgttctt cgaggggagc
     9601 ttctttccaa aagttcttgc gctggtaggc gagagcttct ttttctggtt tagtgttttt
     9661 cattgcttct ccaattgttt tatttattta atttctataa cttttaaatg tctttcaccc
     9721 atgggagcgt gaacaactgc aatttctccg atctttttac ttaaaagggc tctgcctatc
     9781 ggggaaacta cagaaacttc ttgtatgccc tcttcatcgt aataatttaa ttcatcagct
     9841 cctaccagat gataacaaat acattcattg ttgctgatat cttcagcaac acaataagat
     9901 ccaaaacgaa cgatatcttt aggaaattca ctggtatcaa ttacttgcaa tttggcactg
     9961 cggcatctga gataatcaat ttcgttatct atggctcgtt ggcgttctct ggctgcttta
    10021 tattctgcat tttcactgag atcaccaaat tcgcgagcaa tagcaattgc tttaataact
    10081 tccggacgct cggtaagtaa ttcatttatt cgtttctgca gtcgctgcat tccagttttg
    10141 gtaatatatt ggcttaaatc caagtttcaa ttagctcctt tttttcttca ttaaaatgcg
    10201 ttgtgctgaa gtagctgctt tctttaaacg ggcatttcca ctctttgtcc aaatttcgat
    10261 cgaattttcc agctccggat aataatccgt tatagaagca caaagcaatt ctacaatttg
    10321 aggatcacgg ctgctgccat attcccgcca aatatttaca taatattcag gatcgatctt
    10381 agcaacagtt ttcagcattg ctatatgaac agcaatagct tctcccaaag gataaagcca
    10441 ctggtgtata aagtaattaa caacctgggg tataagatct tcccgctttt taattagctt
    10501 tattaataaa tttaaagcag cgctctgtaa atccaaattt tggctgttcc caaattcaaa
    10561 aaccttatcc agatatttat caggatattt acgaataaga ggtaacataa ccttgtccaa
    10621 aagagtattt atctcagctt cgtcagctgt tgaaatcgct ccttccaaaa caggcatata
    10681 cactgaaggc tttcgatgta aaagcttgct aagaatctga gcaaatgcct gcttgccata
    10741 atctcccttc ttcgcccaca atgcattata gaaaggaaga tacaactcat gttttttccc
    10801 taattgctga actatgttat ttgcaacttg agagatgacc tccgctggaa ttttgccatt
    10861 taccctttcc ggatatgcaa taaagataat ttcaaaatca taatcacctt tcggaagctt
    10921 atttttcaga aaatctgcag tatccatatt cagccgttct ttccagtcaa tggtaagcat
    10981 caatctctcc caatatcact tatattttct ctatttggta ataaatcaaa aaattgccca
    11041 atcttgtcaa tagaaaaata caaccgtctt attaacgagg gcattagcaa agcacatact
    11101 atattgtgcc cgcttataaa ttattctacc gcaacccaaa atttaaaaat taaaaacaat
    11161 ggctgggatg gaaggagttg aaccctcaca tacggatcca gagtccgctg tcc
//
LOCUS       DAOSTH010000051        11010 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_5838_length_11010_cov_14.798539, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000051 DAOSTH010000000
VERSION     DAOSTH010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 11010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11010
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5838_length_11010_cov_14.798539"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            759..2126
                     /locus_tag="PLB65_06305"
     CDS             759..2126
                     /locus_tag="PLB65_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOQ78176.1"
                     /translation="MIQNLQLIRDHAQLREMGSPLKYNILKELINAPATCQQLATLFG
                     CSKQKMHYNLTQMLSQGLLRIEDEQNGNNKEVYYRATARSYVLDFAIGLETKEKILDN
                     RRIIGSILQQKYHIDLDQIAAKLIDDSLKLTKGMNLLISTGKFNLPLVEKLLLEAGRR
                     GIYTTLLYQDLDQLKARAEQYSLSAFQADYENFNRKLREADVYLNLNSESRNVEITDP
                     EKFKIRSRMLEKGRRIIKEKQIRLAMMPSLLYDTLSDQAIESEVQFWNALDIDYQLLS
                     DKTIAMCERFVNKDVVEIVGNSASFLFNIDSIWGECGSFGEGKFQSPIINLPGGEILI
                     IPKQGTMQGKIIGDRAFALGEEITRPVVEIANNEIASFSAETNQQQLAKAIEMGGKDG
                     RKVALICLGTNDNIRGGDIDIALKHKSNGFMSVYWGSNREVGGSIAGNIEWFIEIENP
                     QLNFK"
     gene            2142..5402
                     /locus_tag="PLB65_06310"
     CDS             2142..5402
                     /locus_tag="PLB65_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOQ78177.1"
                     /translation="MKKTFLLSLLILCTAFAFGQWQIDEDFEGITTLPTGWTVYDDGD
                     GMTWRNLEEASHAHSGTRFAFADNYFPNQNCDWMITPQLAITSGDSLLFYTRAWYGTE
                     NLKVYVSTTGNAISNFSTQILNLQNLGTTFQYASYNLSAYAGQNIYIGFKWECETYGI
                     VVDDVKIGQPVIVTPELNLPDSYTFWQGETLTVDFTPYIVATDINNVQLSWQTPEHIT
                     ISATGLSVTFSSDNWNGTENITFTLTDNINGVTASDTVQIIVHPIPTVDMALQTINSP
                     SNTEYVNSLFTPTVIVKNNGQVLWEDQLEISFKVYNSQNSLVDSISAYFNPVLEPEAT
                     YEVIFPVMSINTEGVYTATFQINLSDNNPTNDIISREFNVILRINTGGPDAFGFRYID
                     NTAENGPTFDWIDISSTGTSTIMYGVNQFNGDDNFSEPIPFGFDFPFYGVNYSTAYVD
                     INGEILLADNNWYKEYPDSGWGNDGNMFNYMYPIPGYNQMPGLIAVYWDDLHCDQGTG
                     DIYFQSLGEEPNRYCVIQWHNVRFHAGTGISSYLDFEVILHENGEIVMQYNSTATGQT
                     GANVPHDNGKSATVALQSSDATMGICYLREIVQNNAYQGVEPAGNLLFDGLAIRFYSG
                     EDTQAPFITHTAPGNTFNQSPVLEARVIDLSELANVTLHYNYGEGWDTLEGIATGNGY
                     YTFILPQLPTGHTFSYYIASADANENSSTLPAGAPDEVFQFKILPTDGTEVLLAYSGK
                     QDYQHTELPVYVERLENLNISYDLYNWEEYEEYSIPDTYSTIITYACTGSASPATLYF
                     SQKLMDFLNSGTIDNAKNLFFASDGFAFSQAGTPNSHPMKQLLNGYLRTHSVATGSGG
                     GTNGLAGPDVFSYQTGTIISTNSSPIGNAGTEYSVYANSPDCIFAYESVPDWYVDLVP
                     YPEIGAVNAFAFEDGPVDGHAYLYHGVCATAVDTPIFKTFYFSFDYSQLNNPAQSEEL
                     FSDLMEWFGIGPDAVDDVETPVVKSELKANYPNPFNPTTTIVFSLATAEKVEINIYNL
                     KGQKVKTLLSDNLLSGNHSVVWDGKNDKNNSVSSGIYLVKMQTGKTTATRKITMLK"
     gene            5414..7096
                     /locus_tag="PLB65_06315"
     CDS             5414..7096
                     /locus_tag="PLB65_06315"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOQ78178.1"
                     /translation="MRKYVIIILLLFSLSILAAEDYIIGTGTGNQNYIPFYGGNNYSW
                     SRFFFTADELLAAGMTGTVEISKIGFQLAVNTFENYVTNDQRIYIREFYDETYPSSPQ
                     YVNPSGYPIAYQGTISWTSPGWVFIQLTNPYTYTYNGGSPVPNGIEILWENRDGSAQS
                     PYPRFTYTQASENRAAYKYNNDYFPTGSGTKTKSRPNVWFISPATTVPNPAVNPVPAN
                     EATGVEITTNLKWTSGGGDPVDYLVSLYRTDPLTFIVNNQVTTSTTYILPTLLDYSTT
                     YYWRVIPRNSFGNAVACPAWSFTTMADPSIMNYPWTENFDGSSFPPNSDWLRRGGDLV
                     DPIQLGGNSLWEQDDWLNIAGTDKAARINIWGNLNGWLITPQFLFTEDSDYLYFDLAL
                     LKYNQNPNGTPPDTTGLDDRFAVLISDGYTWSTSNIVREWNNTGSPYVLNDINPWGER
                     ICIPLNGLTGHKRIAFFAGSTTSNADNDFMINNLYVGPLVLSKPEVQLSQTGDNNCVL
                     SWNVVYGASGYDVYSSDLPTGPWNFVISTTANQYRFIAGTSKQFFQVKAVSR"
     gene            complement(7362..10307)
                     /locus_tag="PLB65_06320"
     CDS             complement(7362..10307)
                     /locus_tag="PLB65_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOQ78179.1"
                     /translation="MKRLFSLCLFIVIAFAFGAEEICVRRSDYLELSTFHFSTADGNE
                     IVFWNDTVNGSPDIFAQKLDANGLCLWDENLCLTDSPGDQNIIDLVKTSDNNCIILYT
                     DYSLASVTSYYMQKITPDGQCLWSPNGVLFSCEPMKIDTIKLIPNASGGAHILYTSAY
                     HSLYRQMIDANGSYIFSLDNTSIYTDTLYVTLKNAVSDNSGGAIIAISKYLGLNTTKI
                     THLIHLSQEGFITGNNPLVPNADLYTTNYDILKGIDNQIILWGYCSLPSDGIAFRKID
                     TNGNLLSPQPTVYQFPSSVSPYDKISPVATLEGGLLLAWQEWNYTMHQYNIKMQKYDT
                     NLLPVWLESGILVSNYGPRENRFQMLMKNNGNILASWTTVNYSPFSIYYMAQLIDPQG
                     IKCWGENGSIIQELSDYLNYVTSPDGSNNIFVWSDTNNNNISIKRNVVTDAGISLLPP
                     EELTIVEHLGSAAMLFDCVALGDHYLNIWFDSRNNNAIYYQIYNSNQEALFQENGIAL
                     TEPNIGLLGILTTEKISENEYAIIYRIQNDDILYATYLQCIDANGTKLYDGNGILLYS
                     TYGGSFSLSGWDNDIYIGWVLNEDDSNSYVMAQRISGGQKMWGEEGKVIAELSNYVNS
                     VKNRGRYFVWSSTDANFLTSIKALKLDVNGNPVSGWNPEGLNLAQLPETFVAHINNAF
                     LEADNLVLTYSYYNFSNYNELYMAQKINSEGELLWSDNGIQICVEDTYPLDTIWDNNN
                     IIFVSSFYYPANNLLFFNIVNSSGELLYGIEGIPINNNSMCCYECSLLKFANGAYLLT
                     CAYFSTEYDCCDIYNIELDPHGNTVANQVICNAIFDQSGVQSSAIGNQAFLAWNDYRT
                     SVFSYDVFITSIYATWVNSSYVANEDEVITNPAVISLQQNYPNPFNPETTISFSLPKA
                     EKVNLNIYNLKGQLVKTLYQDNQCSAGVNSVVWNGKDNKGNSVSSGIYFYRLNCGKEQ
                     LTKKMVLSK"
BASE COUNT         3497 a         1923 c         2175 g         3415 t
ORIGIN      
        1 acaagaagaa ccaataaaaa aagtcccagc gggacgacag atttagtttt agaaacgagg
       61 attttaggat taagaggatt tatttataag ccaccgaagt caccgaagat ttatcacgca
      121 gattatacag aaagaagtct ctcacagatt acacagatta cacagattta gttttagaaa
      181 caaggatttt aggattggat tttcgaaaca aggattttag gtaggatttt ttaacgcatt
      241 cctttttcct taaattaagt ccactgtttt caagaggatt cgaacaaata acccctcctc
      301 atctcctcct cctcccgtct tcaacccata gttaaggagg agaggagatt gggtggagga
      361 caaattgtat atcgttaact tacaagcgaa agtaaggctt agggcaaaac agttatttat
      421 aacttattaa gattattgga cttaatagat atcaacagct tcgaataatc gatcttatta
      481 tgctttaatt atttttgttg agcaatccac tagatattgg gagaggttgt atctccaaag
      541 gttggcataa aagaaaaagc gggaaagtaa attggcgatg tacaacaatt ttacaatatt
      601 ggtggagctg aaacataaag tagagaacag gctgagggga tacaggcaaa gcaatttagg
      661 agaaaacatc attttttgtc aaaaataact tgactaaata ttaaggatgg atgataagga
      721 ttttatataa aacaaaacta acgagaagga aaaatacaat gattcaaaac ttgcagttaa
      781 taagggatca cgctcaattg agagagatgg gcagtcccct aaaatacaat attcttaaag
      841 aattgataaa tgccccggct acttgtcagc agcttgctac tctttttgga tgcagtaaac
      901 agaagatgca ttacaattta actcagatgc tatcgcaggg tttattaaga atagaggacg
      961 agcaaaacgg aaacaacaaa gaggtatatt atagggctac ggcacgcagt tatgtacttg
     1021 attttgcgat aggtttggag accaaggaaa aaatattgga taatcgcagg attatcggta
     1081 gtattttaca gcagaaatat cacatagatt tggatcaaat tgcagccaaa ttgattgatg
     1141 attcattgaa gttgacgaag gggatgaatt tgcttatatc tacaggcaag tttaatttac
     1201 ctttggtgga aaagttattg ctggaagcag gaaggcgtgg aatttacact acattgttat
     1261 atcaggattt agatcagcta aaagccaggg ctgagcaata ttcgctttcg gcatttcaag
     1321 cggattatga gaatttcaat agaaagctga gagaagcgga tgtatatttg aacttgaata
     1381 gtgaatcccg gaatgtagaa attactgatc ctgaaaagtt taaaatccgt tccaggatgt
     1441 tggaaaaggg tagaaggatt atcaaagaaa agcagattcg tttagcaatg atgccttctt
     1501 tattatacga cacgctatcg gatcaggcaa ttgagagtga agttcagttt tggaatgctt
     1561 tagatataga ttatcagtta cttagcgata agacgattgc gatgtgcgaa agatttgtaa
     1621 ataaggatgt tgtagaaatt gtagggaatt cggcttcttt cctttttaat atagatagta
     1681 tctggggaga atgcggttct tttggagaag gcaaatttca gtctccgatt atcaaccttc
     1741 ctggaggtga aattttaatt attcccaagc agggaacgat gcaaggaaaa ataattggag
     1801 atcgtgcttt tgctttgggg gaagaaataa cgcgtcctgt ggtggaaatt gcaaataacg
     1861 aaattgccag tttttctgcg gaaaccaatc aacaacaatt agcgaaagca attgagatgg
     1921 gtggaaaaga tggcagaaag gttgctctta tttgtctggg aaccaacgat aatattcgtg
     1981 gtggagatat agacattgct ctaaaacata aaagcaacgg atttatgagt gtttattggg
     2041 gaagtaatag agaagtgggt ggttcaattg ctggaaacat tgaatggttc atcgaaatcg
     2101 aaaatcccca gcttaatttt aaataaaaaa agaggtctat tatgaaaaaa acatttttgc
     2161 tatcactcct gattctatgt actgcttttg cttttgggca atggcagata gatgaagatt
     2221 tcgaaggaat tactactctt ccaacaggtt ggacagttta tgatgatgga gatgggatga
     2281 cttggcgcaa tctggaagaa gccagtcatg cacattcggg aacgcgtttt gcgtttgccg
     2341 ataactattt tcctaatcag aattgtgatt ggatgatcac tccccaactt gccattacta
     2401 gtggtgattc tctattattt tatacgcgtg cctggtatgg aactgaaaat ttgaaagtat
     2461 atgtttccac aacaggtaat gctattagta atttcagcac tcagatattg aatttgcaga
     2521 acttaggaac tacttttcaa tatgcaagtt ataacctttc tgcttatgct ggacaaaata
     2581 tttacatcgg gtttaaatgg gaatgcgaaa cttatggtat tgttgtagat gatgtaaaaa
     2641 tagggcaacc tgtaattgtt acgccggaat taaacctgcc tgatagttat actttctggc
     2701 aaggagaaac cttaacggta gattttactc cgtatattgt agcaacggat attaataatg
     2761 ttcagctcag ttggcaaact ccagaacata taactatttc tgccacgggg ttatcggtaa
     2821 cttttagcag tgataactgg aatggaactg aaaatatcac ttttactcta actgacaata
     2881 ttaatggagt tacagcatcg gatactgttc agataattgt gcatccaatt ccaacagtag
     2941 atatggcttt gcagacaatt aattctccca gtaatacaga atatgtaaat tccttattta
     3001 ctcctactgt gattgtaaaa aataatgggc aagtattgtg ggaagatcag ctggaaataa
     3061 gcttcaaagt gtataacagc caaaattctc ttgttgatag catttctgct tattttaatc
     3121 ctgttttgga acctgaggct acatatgaag taatttttcc tgttatgagt attaatactg
     3181 aaggagtata tactgctact tttcagataa acctaagtga taacaatccc actaatgata
     3241 ttataagccg tgaatttaat gtcatcctga gaataaatac gggcggacct gatgcttttg
     3301 gttttcgcta tattgataac acagcggaaa atggacctac ttttgattgg atagatataa
     3361 gctctacagg tacttctaca attatgtatg gagtgaatca atttaatggt gatgataact
     3421 tttctgaacc tattcccttt ggttttgatt ttcccttcta tggagtaaac tattctactg
     3481 cctatgtgga tataaatgga gaaattctat tagctgataa taattggtat aaagaatatc
     3541 ccgacagcgg atggggaaat gatggaaata tgtttaacta tatgtatccc attcctggat
     3601 ataatcaaat gccaggttta atagcggttt attgggatga ccttcattgt gatcaaggaa
     3661 cgggagatat atattttcaa agcttgggag aagagcccaa tcgttattgt gtgattcaat
     3721 ggcataatgt aagatttcat gctggaaccg gaatttcctc ttatttggat tttgaggtta
     3781 tcttgcacga aaacggggaa atcgttatgc aatataattc tacagccact ggacaaacag
     3841 gagccaatgt tcctcatgat aatggaaaaa gtgctaccgt tgctttacaa agttcagatg
     3901 ccacaatggg aatttgctat ttaagagaaa ttgtgcaaaa taatgcctat cagggtgtgg
     3961 agccagcggg aaatttactg tttgacggtt tggcgattcg tttttatagt ggtgaagata
     4021 cccaagcgcc tttcattaca catacggctc ctggtaatac ttttaatcaa tcacctgttt
     4081 tagaggcaag agttatagac ctttctgaat tggccaatgt tactctgcat tataattatg
     4141 gagaaggttg ggatacgctg gaaggaattg ctacaggaaa cggctattac acatttattt
     4201 tacctcaact acctactggg catactttta gctattatat tgcttctgca gatgctaatg
     4261 aaaatagttc aactttgcca gcaggggcac cagatgaggt ttttcaattt aaaattcttc
     4321 ctactgacgg aacagaagtc ttacttgcct atagtggaaa acaggattat cagcatactg
     4381 aattgccagt ttatgttgag cgtctggaga atttaaatat ttcctacgac ctctataatt
     4441 gggaagagta tgaggaatat tctattcctg acacttactc aactatcata acttatgcct
     4501 gtacgggctc tgccagtcct gctactctat atttttcgca gaaattgatg gattttttga
     4561 attcgggaac aatagacaat gccaaaaacc tgttttttgc ctcagacggt tttgccttca
     4621 gtcaagcagg aacccctaat tcgcatccga tgaaacagtt acttaatggt tatttaagaa
     4681 cacattccgt tgctacaggt tctggaggtg gaactaacgg tttggcagga cccgatgttt
     4741 tcagctatca aaccggcaca attattagta caaattcttc tccgattggt aatgcaggta
     4801 ccgaatatag tgtttatgcc aatagtcccg attgcatttt tgcgtatgaa agcgttccag
     4861 attggtatgt agatctggtt ccctacccgg aaattggagc agtgaatgct tttgcctttg
     4921 aagatggacc tgtagatgga catgcttatt tatatcatgg agtttgtgct actgctgtgg
     4981 atacaccgat attcaagaca ttctactttt cttttgacta ttctcagctg aataatcctg
     5041 ctcaaagtga agaactattc agcgatttaa tggagtggtt tggaattggc cctgatgctg
     5101 tagatgatgt ggaaactcct gtggttaaaa gtgaattgaa ggctaattat cctaatccgt
     5161 ttaatcctac aactaccatc gttttctcgc tggcgacagc ggaaaaagtt gaaatcaata
     5221 tctataatct gaaagggcag aaagtgaaaa ccttgctctc ggacaatctg ctttctggaa
     5281 atcatagtgt ggtttgggat ggtaaaaacg ataaaaataa ttctgtaagc agcggcatat
     5341 atctggttaa aatgcaaact ggaaaaacta ctgctacccg taagataact atgctcaaat
     5401 aggaagtaca tttatgcgta aatatgtaat aattatcctg ctgctgtttt cgctatcaat
     5461 tctggcagca gaagattaca ttattggtac tggaacagga aatcaaaatt atattccctt
     5521 ttatggtggt aataattaca gttggagcag gttctttttt actgcggatg aattactggc
     5581 ggcaggaatg acaggaactg ttgagatttc caaaatcggc tttcagctgg cagtaaacac
     5641 ttttgaaaat tatgtcacaa acgaccaaag aatatatatt cgtgagttct atgacgaaac
     5701 ttatccttcc agtccacaat atgtgaatcc ttctggatat ccgattgcct atcagggaac
     5761 tatttcttgg acaagccccg gctgggtttt cattcagctg acgaatccat atacatatac
     5821 ctataatggc ggtagtcctg tccccaatgg aattgaaatt ctctgggaaa atagagatgg
     5881 ctccgcacaa agtccctatc caagatttac ttatacccaa gcaagtgaaa acagagctgc
     5941 ttataaatat aataatgatt attttcctac tggtagcgga accaaaacta aaagcagacc
     6001 taatgtctgg tttatttctc ctgctactac agttcccaat ccggctgtaa atccggtgcc
     6061 ggcaaatgaa gcaaccggag tggagataac tacgaatctg aaatggacga gcgggggagg
     6121 tgatcctgtt gattaccttg tctctttgta tagaactgat cccttaacat ttattgtaaa
     6181 taatcaagta actacttcca ccacatatat tttgccgact cttttggatt atagcactac
     6241 ttactattgg agagtaattc cccgtaatag ttttggtaat gcagttgcct gtcctgcttg
     6301 gagttttaca actatggcag atccttctat tatgaattat ccttggacgg aaaattttga
     6361 tggaagttct tttcctccaa attcagattg gttgcgtaga gggggtgacc tggttgatcc
     6421 aattcaattg ggaggtaact cattgtggga acaggacgat tggctaaata ttgcaggaac
     6481 ggataaagca gccagaatca atatctgggg caatttaaat ggctggctta tcactcctca
     6541 atttttgttt actgaagata gtgattatct ctattttgat ctggcattgc tgaaatataa
     6601 tcaaaatcca aatggaactc ctccagatac aactggactt gatgatcgct ttgccgtttt
     6661 aatttccgat ggttatactt ggagcacttc taatattgtg cgagaatgga ataatacggg
     6721 ttcaccttat gttctgaatg atattaaccc ttggggggaa aggatttgta ttcccttaaa
     6781 tggtttaaca ggacataaga gaattgcctt ctttgctggc tctacaactt ctaatgctga
     6841 taatgacttt atgattaata acctatatgt gggacctctg gttctaagta aaccggaagt
     6901 gcaactatct caaacaggtg ataataactg tgttctaagc tggaatgttg tttacggggc
     6961 aagtggctat gatgtttact cttccgattt gcctactggt ccttggaatt ttgttatttc
     7021 cacaacggca aaccaatatc ggtttatagc tggaacatct aaacaattct tccaagtgaa
     7081 agcagttagt agataattat gtaaaacctt atctccttcc acaaaaaccg gctgaagttt
     7141 ttttcagccg gttttttgca gctatgaaag caacgaaaaa agaactctga actcctgcac
     7201 tttgaacttt gaactcccgc actttgaact tcgaactttg aactcccgca ctttgaactt
     7261 cgaactttga actcccgcac tttgaacttt gaactttgaa ctcctgcact ttgaacttcg
     7321 aacttccgca ctttgaactt tgaactttga actcccgcac tttatttact aagcaccatt
     7381 ttcttggtca gctgttcttt tccacaattt aggcggtaga aataaatgcc gctgcttacg
     7441 ctatttcctt tattatcttt tccattccag accacagaat ttactcctgc agaacattgg
     7501 ttatcttggt aaagggtctt aaccagttgt ccttttaagt tatagatatt caggtttact
     7561 ttttccgctt taggcagaga gaaagaaatc gtggtttccg gattaaaggg attagggtaa
     7621 ttttgctgta aagaaataac ggcaggattt gtgataactt catcttcatt tgccacataa
     7681 gaactattca cccaagtggc gtaaatggag gttataaaca catcataact aaatactgaa
     7741 gtccggtaat cattccaagc aagaaatgct tgattaccga tagctgaact ttgcacacca
     7801 ctctgatcaa atatagcgtt acaaataact tgattggcaa cagtattacc atgaggatca
     7861 agctcgatat tatatatatc acaacagtca tattcggttg aaaagtatgc gcaggttaaa
     7921 agatatgcac cattggcaaa tttcagcaaa gagcactcat aacaacacat tgaattatta
     7981 ttaatgggta ttccttcaat accatataaa agctcacccg aactatttac aatattaaag
     8041 aagagaagat tattggcagg gtagtagaaa gagctaacaa agattatgtt attattatcc
     8101 caaatagtat caaggggata ggtgtcttct acgcaaattt gaataccgtt atcagaccaa
     8161 agtaactctc cttcgctatt gattttttga gccatatata attcgttata attgctaaaa
     8221 ttataatagg aataggtaag aaccagatta tctgcttcta aaaaggcatt attgatatgg
     8281 gcaacaaatg tttcgggaag ttgagcaagg tttaatcctt ccgggttcca tccggaaaca
     8341 ggattaccgt ttacatccaa ttttaacgct tttatagaag taagaaaatt ggcatcggta
     8401 gatgaccaaa cgaaatacct tcccctattc ttaacactat tcacataatt acttaattca
     8461 gcaattactt ttccttcttc tccccacatt ttttgtccgc ctgatattct ttgtgccata
     8521 acataactat ttgagtcatc ttcgtttaat acccaaccga tataaatgtc attatcccag
     8581 ccggataaag aaaaagatcc tccataggta ctatacagca aaataccgtt tccgtcatac
     8641 aattttgtgc cgttggcatc aatacactgc aagtaggttg cataaagaat gtcgtcattt
     8701 tggattctat aaataatggc atattcgttt tccgagatct tttccgtagt taggattccc
     8761 aacaaaccaa tatttggttc tgttagggct atgccatttt cctggaataa tgcctcctgg
     8821 ttactgttat atatttgata ataaattgcg ttgttattgc gagaatcaaa ccagatattc
     8881 agatagtgat ctcctaaagc tacacaatca aaaagcattg ctgcacttcc taagtgttct
     8941 actatagtta attcttccgg aggcagtaaa ctaattccgg catcggttac aacattgcgt
     9001 tttatgctta tgttattgtt atttgtatca gaccaaacga atatattgtt gctgccatca
     9061 ggggatgtaa cataattcag ataatcagat aattcctgaa ttatactgcc attttcaccc
     9121 cagcatttta ttccctgtgg atcaatcaac tgtgccatat agtaaatact gaaaggagaa
     9181 taattcacgg tggtccagct tgccaagatg ttgccgttat ttttcattaa catttgaaat
     9241 ctattttccc taggaccata gttacttacc aaaattcccg attccagcca gacaggaagt
     9301 aggtttgtat cgtatttttg cattttaata ttatattgat gcatcgtata attccattcc
     9361 tgccaggcaa gtaataaacc tccctctaaa gttgccacag gagaaatctt gtcataaggt
     9421 gaaactgaag aaggaaactg ataaacggta ggttgagggc ttaacagatt accatttgta
     9481 tcaatttttc taaaggcaat gccatcgctt gggagagaac aatatcccca taaaataatc
     9541 tgattatcaa tacctttgag gatatcataa ttagttgtat aaagatctgc attaggaact
     9601 aaagggttat taccagttat aaaaccttcc tgtgagagat gaataagatg agttattttt
     9661 gttgtattta aacctaaata cttactaata gcaataatcg ctcccccgga attatcacta
     9721 acggcatttt tgagcgtgac atacaatgta tcagtatata tagaagtgtt atccagagaa
     9781 aaaatataac tgccatttgc atcaatcatc tgcctgtata aagaatgata agctgaagtg
     9841 tataaaatat gcgcgccccc agaggcatta ggaatcagtt ttatcgtatc tattttcata
     9901 ggttcacaag aaaatagaac accgttggga gaccataaac attgtccgtc gggtgttatt
     9961 ttttgcatat aataacttgt gactgaagct agtgaataat cggtgtataa gataatgcaa
    10021 ttattatcgg aagttttcac cagatcaata atattctgat ctccgggact atctgtcaga
    10081 cagaggtttt catcccacaa acagagtcca ttagcatcca gtttttgggc aaagatatcc
    10141 ggactcccgt taactgtgtc attccaaaaa acaatttcat tgccgtcagc tgtagaaaaa
    10201 tgaaaagtag ataattccaa ataatcagat cttctaacac aaatctcttc tgccccaaag
    10261 gcgaaagcaa ttacgatgaa taaacataag gaaaacaagc gtttcatttt atcctccctt
    10321 ttattatctt ttataatgag atatatattt atgcaagaaa tgaaccatta gatagatatt
    10381 tttcccaaag aaaataaaaa ctatctatcg gctacaccga agcagatcat tgcaccctga
    10441 ttacacttat aacgcagtaa catatccgta acaatcaagt aacgagacaa ctgcagtgtt
    10501 acggaagtgt tactgggttg ttagagaatt tactatggga caagcaatta ggatattctt
    10561 gccaatgtcg caaaatcatc cggtaattat ggcagaagat tgagggtagg aatagacgga
    10621 aagataagga tttcggagca gaaatggata tgacactgaa caagataaaa aaaagggcaa
    10681 tgcccaacaa gcgttatagt aaattacctt tgggctttgc taccttttag cttttctcta
    10741 tctctaatgt ggtgtccgat tgttttagct atgtaacaaa ttcctacatc aataaatatg
    10801 aagggaaatg ggttgaggca caacgactta tactaccctg ttctaaaaat ggggggatct
    10861 gaactgaaga aaataagcgc ttgacaatct ggatgataat aaaaaagaat aattttgcct
    10921 aaatagttag gagttgaaag atggatatgt ccagtacaag attgtggatc atcataccca
    10981 aagatttctc ggaatcggtg ccaaagctat
//
LOCUS       DAOSTH010000052        10917 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_5890_length_10917_cov_11.571442, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000052 DAOSTH010000000
VERSION     DAOSTH010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5890_length_10917_cov_11.571442"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(286..1059)
                     /gene="lpxA"
                     /locus_tag="PLB65_06325"
     CDS             complement(286..1059)
                     /gene="lpxA"
                     /locus_tag="PLB65_06325"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOQ78180.1"
                     /translation="MTTIHPTAIIEEGAVIGENCNIGPYCNIGKYVVLGANNHLIANV
                     TISGHTTIGDGNKIYPFAVLGTEPQDLKYKGEQTRLQIGSNNTIREFVTINCSNQIDE
                     DTVVGNNNLIMEYVHIAHNCQIGSHCVIANVVQFGGHVHINDYASVGGLTAVHQFVHI
                     GSYAFVGGASAVNKDVVPFSRGQGNLYKSVGLNTIGMMRKGFSTETIAKIKEIYNLFY
                     RSGLNTSQAIEKALQIPNPTPEQLIFIEFVQNAQRGISK"
     gene            complement(1056..2450)
                     /locus_tag="PLB65_06330"
     CDS             complement(1056..2450)
                     /locus_tag="PLB65_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HOQ78181.1"
                     /translation="MEYKQTIGGESSYTGIGLHTGEISTIRFKPAGPEEGIVFIRTDL
                     PGKPEIPADIDHVVDISRGTTIGINGATVATIEHVLSAVVGMRIDNIRIEINGPEAPV
                     ADGSAVVFLNILRQCGIMQQDSEREYFELESPISFSAPEDNVDIVIVPSNELKITFMI
                     DYKHPYLGTQYTWLPSLDYYEKDFAGARTFCFINEILALKNMGLIKGGSLENALVIAE
                     PDISEEELKHLQDVFGYHQPITVSSEGILNSHPLRYYNEFVRHKVVDLIGDIALLGIP
                     IKGHILAARSGHKTNVELVKKLRQIQKKQQLQKIYQKGKAKDVIFDINAILRILPHRY
                     PFLLVDRIVEFTPGETLVGIKNVTINEPFFQGHFPGHPIMPGVLIIEGMAQAGGIMLL
                     NQMDNPQDYVAYFASIDNVKFRKPVLPGDTLRYELKVISLKKSLAKMHGETFVDGEKV
                     AEGDFMAMIMEREK"
     gene            complement(2450..3499)
                     /gene="lpxD"
                     /locus_tag="PLB65_06335"
     CDS             complement(2450..3499)
                     /gene="lpxD"
                     /locus_tag="PLB65_06335"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOQ78182.1"
                     /translation="MKRFSTNISEEILLHICQGELIGKIPENLCKVSEPQEADQQTII
                     FLEQEKLLDIVKASSAGLIITTKRYVPMFPDRALLIVEKPYFALMLLITYLLNQENKN
                     ITYTIHPSAVVAEDVILDKEVAIGANVVIGSGCKLGKGVIIGEGCSIGKNVYIGERTK
                     LYPNVCLYDDCVIGNNCILHSGVIIGADGFGFMLIAGVQQKIPQVGNVVIGNDVEIGA
                     NSCIDRATLGSTIIGNNTKIDNLVQIGHNCVVGEHSVICSQVGLAGSTIVGDFVYLAG
                     QVGVADHLKIGNRAMIGAQSGVSSNIPDDGKYFGYPALDANLTKRIMAVQKNLPEMYF
                     AYLKAKKKETNSGDK"
     gene            complement(3496..4122)
                     /locus_tag="PLB65_06340"
     CDS             complement(3496..4122)
                     /locus_tag="PLB65_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOQ78183.1"
                     /translation="MKKHILVLIVLVASFALAYAQPVKLGFVNTDRLLLESNEAAEVA
                     RLFALDKQNWTNQIRTLDDEIKQMERDFEIRKLTMNDAAKRETQSRIDTKKTEAGRLL
                     EEYFGDGGKAEQRYRELIDPLTLKIHNLIKKIAEDEKYTMIFDVSMGSLLYALPTLDL
                     TEQILLELNKDTVKPTDVSPTDINPPKDQTPPEIKSPEGYEEFKPDKK"
     gene            complement(4153..5607)
                     /gene="gcvPB"
                     /locus_tag="PLB65_06345"
     CDS             complement(4153..5607)
                     /gene="gcvPB"
                     /locus_tag="PLB65_06345"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HOQ78184.1"
                     /translation="MSKTIFEHSSPSRKGVTLPKREIDTLLPTLIPETMLRTKKARLP
                     EVSELDVMRHYISLSHKNHFIEKGLYPLGSCTMKYNPKLNETLVRHSSFTTLHPFQPE
                     ETMQGSLELMYELQNDLAEISGMKGVTLQPSAGAQGEFTGIKIIAAYHKAKGNNHKDT
                     IIIPDSAHGTNPASCSLVGYKTVELASNKEGRCDIAKLRELVNENTAGIMITNPSTLG
                     LFETQIEEIAEIIHSVDALMYMDGANLNALLGIVQPGKIGFDLMHFNLHKTFATPHGG
                     GGPGSGPVAVNEKLLPFLPVPIVTKDECGYHLSYENEATSIGKVHSFYGNFAVLIRAY
                     IYIKMLGAEGLRRVSENAILNANYLMKILGDYYHIEHQENCMHEFVMDGTRQKKEYGI
                     TTLDIAKRLLDKGFHSPTIYFPLIIPEAMMVEPTETECLESLDAFAKAMIEIAEECKE
                     NPELVKEAPLTTPVKRVDDVRAVKVLEPIFPLPN"
     gene            complement(5604..6971)
                     /gene="gcvPA"
                     /locus_tag="PLB65_06350"
     CDS             complement(5604..6971)
                     /gene="gcvPA"
                     /locus_tag="PLB65_06350"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HOQ78185.1"
                     /translation="MSIKQGAKMPYISNTDNDRRKILKVIGANKFEDLISAIPAKLRM
                     CNPLAISEPLSELEINRNIQTKADKNKPCIKVNSFLGGGVYDHFIPAAVDAIVSRPEF
                     FTAYTPYQAEVSQGTLQYIFEYQTMICELTGMEIANAGMYDAASSIAEAILMAVRKNH
                     LTKAILPATLHPNYVKVIKAYTEGIGIELLTVPEKEGVTDLSALEAMMDETIGSVVIQ
                     TPNFYGNLEDAKAIDTIVHKNSKCLLIASVDPVSLAVLIPPSEYNADIAVGEGQALGN
                     NMYMGGPLFGFFATKLDMARLMPGRIVGGTIDKAGEKAYVLTLQAREQHIRRAKATSN
                     ICSNESLCTLAAVVYLCLLGKEGLIEVATQSVQKAHYAASELCKIPSLALAYPKATFF
                     KEFVIRTPKKAADIINALLPENIYAGIDLAPYGKPNELMIAITEKKSKTEIDSFVQAM
                     KEVVK"
     gene            complement(7783..10359)
                     /locus_tag="PLB65_06355"
     CDS             complement(7783..10359)
                     /locus_tag="PLB65_06355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HOQ78186.1"
                     /translation="MKKCTCLLLCIIPILGFAAPFYNIPSQAIQPDGSVLNLYASGDE
                     YSNRLHDANGFTIIQSPDDGYYYYAILENGEPVPSAYRFGTIDPASYGITPNINISKG
                     ARQRKINFMNAHKRGNERGPSTGVVNNLCVYIRFSDQTEFEIPRTVYDARFNEVGDDA
                     RSLRSYFQKASYNQLHYMTYHYPVCPDIFNLSYQDSHPRSYYLPYNYITNPGGYHNED
                     ERCIREQTLLMNAINSIASQVPSSLNIDADNNGTVDNVCFIIRGPHSAWADLLWAHRW
                     SLFYTSAYINNKEVYDFTFQPEDQNDVTTLCHEMFHSVGAPDLYHYEYDGLTPVGCWD
                     LMESGDGHMGMYMKYAYGGWIPSIPLAQIGNTYTLNPVTSAENNVYKYPIPGSTNQFL
                     IFEYRKKSSDIFEENLPNSGLLIYRIDQTMDGNVEGPPDGVYVYRPDGSLTNNGLVAE
                     APFSANNFRTAFNEYTNPSSFLNNGSTFPVNITNITEAGETISFTIFSPAVTMAPVID
                     AIHPANGSILADNTLNIYLQVTDPAEAIQRVEYTLDGILVYTATAEPYTGIISDYLLT
                     PGVHNIGITAFSASNLSTTVNINYRIVNPDLENWFSWLSSEPHWIEYGRGAIPIKAAI
                     DMDLGNQEYIVKALRFNITPDPWGEPDMPGMVYARINRFSNGAITDQLLLDIGYCYNF
                     DYDPNFTFNVVDTTHISGQIAVILDLYEYQNMRFDRNAACGHSWIYEPNRPWTDALGR
                     GIVGAAAIELLLQNPNVSIEEPNCPALNISLSSYPNPFTSTTKIKYSLPASGKVNLTI
                     YNLKGQKVKNLLTENKTVGNYEMEWNGTDTQGKNVGSGLYLCRLVSGNRTATTKLLRL
                     SD"
BASE COUNT         3402 a         2146 c         1887 g         3482 t
ORIGIN      
        1 cttggacatt tccaaagcca gtccatcgta tctcgccacc aatcaaagcc ttatctccac
       61 tttcccctcg tgtcacaact tgcagctttg ggtggcagtt tataatcgtt attctaagaa
      121 gtaaggattc gtttcgcatt ttctatcatc attcattatt ttagcctcgt ctatcattca
      181 tcttataaga gaaaaaatct gccattgctt ttatataata tggggtgaca agtttatttg
      241 tgtcacccct taaccctata cagggaaaca taattgctat ttattttatt tagatatacc
      301 tctttgggcg ttttgcacaa actcgataaa gatcagttgt tccggagtag gattaggaat
      361 ttgcagagct ttttctattg cctgagaagt atttaatcca ctgcgataga acagattgta
      421 tatttccttt atcttagcta tggtctcggt gctgaaacct ttccgcatca ttccaatagt
      481 atttaaacca acagatttat aaagattacc ctgtcctcga gaaaaaggaa caacatcttt
      541 attaacggca gaggcaccgc ccacaaaagc ataagaacca atatgaacaa attgatgaac
      601 tgctgttaag ccaccaacac tggcataatc attgatatga acatggccac caaattgaac
      661 tacattggca attacacaat gggaacctat ttggcaatta tgagctatat gcacatattc
      721 cattatcagg ttattatttc caacaacagt atcttcgtct atctggttgc tacaattgat
      781 agtaacaaat tcccgaatcg tattattact gcctatttgt aagcgagttt gttcaccttt
      841 atattttaaa tcctgaggtt cagttcccaa aacagcaaag ggataaattt tattgccgtc
      901 gccaatagtt gtatgtccgg aaattgtaac attggctatc agatgattat tagccccaag
      961 cacaacatat ttaccaatat tacagtatgg tccaatattg caattttcac ctataacagc
     1021 tccttcttca ataatagcag taggatgaat agttgtcatt tttctctctc cataatcata
     1081 gccataaaat cgccttcagc tactttttct ccatctacaa atgtctctcc gtgcattttt
     1141 gccaaagatt ttttgaggga aatcactttc agttcataac gaagtgtatc accgggcaaa
     1201 actggtttgc ggaattttac attatcgata ctggcaaaat aggcaacata atcttgaggg
     1261 ttatccattt gattaagtaa cattattccc ccggcttgag ccataccttc aataatgagt
     1321 actcctggca ttattggatg ccctggaaaa tggccttgaa agaagggttc gttgatagtt
     1381 acatttttaa tgcctaccaa ggtttcacct ggggtaaatt ccacaattct atccacaagt
     1441 aaaaaaggat aacgatgagg taaaatacgc aagatggcat taatatcgaa gatcacatct
     1501 tttgccttac ctttctgata tatcttttgc agctgctgtt ttttctggat ctgacgcaac
     1561 tttttaacca gctccacatt ggttttatgt ccactccgtg cagccaaaat atgcccttta
     1621 ataggaatac ctaaaagagc tatatctcca attaaatcga caactttatg gcgaacgaat
     1681 tcattgtaat aacgtaaggg atgactgttc aaaatgcctt cagaggaaac agttatcggt
     1741 tgatgataac caaaaacatc ttgcaagtgc ttcaattctt cttcgctgat atctggttct
     1801 gctataacta aagcattttc cagagaacca cctttgatca gtcccatatt ttttaatgcc
     1861 aaaatctcgt ttataaagca aaatgtgcga gctccggcaa aatccttttc ataataatcc
     1921 aaagaaggca gccaagtata ttgagttccc agatagggat gtttataatc aatcataaag
     1981 gttattttca gttcgttgga aggaacgata acaatatcta cattatcttc cggagcagaa
     2041 aaactaatgg gtgattccag ctcaaaatat tcccgttcac tatcctgttg cataatacca
     2101 cattgcctta atatatttaa gaaaacaaca gcagaaccat cagctacagg agcttctggt
     2161 ccatttatct caatgcggat attatcaatt cgcataccta caacggctga aagaacatgt
     2221 tcaattgtgg caactgtggc tccattaata ccaatagttg tccccctgga aatatctact
     2281 acatgatcaa tatcagcagg aatttcaggt tttcctggta agtccgttct gataaaaaca
     2341 atcccttctt ccggtcctgc aggtttaaat ctaatcgtgc taatttcccc agtatgaagt
     2401 ccgataccag tataagaaga ttcgccgcct attgtttgtt tgtattccat tatttatctc
     2461 cgctatttgt ttcttttttc ttggctttca ggtaggcaaa atacatttct ggtagatttt
     2521 tttgaacagc cataatgcgt ttagtaagat tcgcgtccaa agctggatag ccaaaatatt
     2581 tcccatcatc aggaatattg ctggatactc cagattgagc tccaatcatt gctctattgc
     2641 caattttaag gtgatctgcc accccaactt gacctgccag ataaacaaaa tctcctacaa
     2701 tcgtgcttcc cgctaaacca acttgggaac aaataacact atgttcacca acaacgcaat
     2761 tatggcctat ttgaaccaga ttatctattt ttgtgttatt tcctattatg gtagaaccta
     2821 aagtagccct atctatgcaa ctattggctc cgatttcaac atcgttgcca ataacaacat
     2881 ttcccacctg gggaattttt tgttgaacgc cagcaattaa cataaagcca aaaccatcag
     2941 ctccaataat tacgccactg tgtaaaatac agttattacc aattacgcaa tcatcataaa
     3001 ggcaaacatt aggataaagt ttcgttcttt ctcctatgta cacatttttg ccaattgagc
     3061 aaccctcacc aataataact cctttgccta atttgcaacc actaccaata acaacattag
     3121 ctccaatggc cacttcttta tctaaaataa catcttcagc aacaacagca gagggatgaa
     3181 tagtataggt gatattcttg ttttcttgat taagtagata tgtaattagt agcattaagg
     3241 caaaataggg tttttccaca atcagcaaag ctctatccgg aaacataggt acatatcttt
     3301 tagtagtaat aattaggcct gcagagctgg ctttaacgat gtctaatagc ttttcctgtt
     3361 ccagaaagat tatggtttgt tgatctgctt cttgtggttc actaacttta caaaggtttt
     3421 caggtatttt gcctataagt tccccttggc agatatgaag gagtatttcc tctgagatat
     3481 ttgtgctaaa gcgtttcatt ttttatctgg cttaaattct tcataaccct caggggattt
     3541 aatttcggga ggagtttgat ctttgggagg gttaatatcc gttggtgata catctgttgg
     3601 ttttacggta tctttattca gttccaacaa tatttgctcg gttaaatcta aagtgggaag
     3661 tgcataaagt aagctaccca tactaacatc aaagatcata gtgtattttt cgtcttcagc
     3721 aattttcttg atcaggttat gaattttcag agttagggga tcaataagtt cacgataacg
     3781 ctgttccgct tttcctccat cgccaaaata ttcttccagc aagcgacctg cctctgtttt
     3841 tttagtatcg attctgcttt gagtttctct cttagcagca tcgttcatag ttagcttacg
     3901 gatttcaaaa tcgcgttcca tttgcttaat ttcgtcatcc aaagtacgaa tttgatttgt
     3961 ccagttttgt ttatccaaag caaacaacct ggctacttct gccgcttcat tactttccag
     4021 gagcagacgg tctgtattta caaaaccaag ttttacaggt tgtgcataag ccaaggcaaa
     4081 cgatgccacc agaacaatta ggactaggat atgttttttc attttttctc cttattgata
     4141 aacggacttt aattaattgg gaagcggaaa gattggttcc agcactttta cagcacgcac
     4201 atcatccact cttttaactg gcgtagttaa tggtgcttct ttaacaagtt caggattctc
     4261 tttacattct tcagcgattt cgatcatcgc tttggcaaag gcatccaaac tttccaagca
     4321 ttccgtttca gttggttcca ccatcattgc ttcaggaata ataagtggaa aatagatagt
     4381 gggggaatgg aatcctttat ctaaaagccg tttagcaata tctaaagtag tgattccata
     4441 ttccttcttt tgcctggtac catccatcac aaattcgtgc atacagtttt cctgatgttc
     4501 gatgtgataa taatctccca gaattttcat aaggtaatta gcatttaaaa ttgcattctc
     4561 agaaactctt cgcaatcctt ccgctcctaa catcttaata tagatatagg cacggattag
     4621 gacagcaaag ttaccataaa aactatgcac tttacctatg gaagtagctt cattttcata
     4681 agaaagatga tagccacatt cgtctttcgt aactatagga accggcaaaa aaggtagcaa
     4741 tttttcgttt acagctaccg gacctgaacc tgggccacct cctccatgtg gagtagcaaa
     4801 ggttttatgc aagttaaaat gcatcaagtc aaaaccgatt tttccaggtt gtactattcc
     4861 caaaagagca ttcaaattgg ctccatccat atacattagg gcatctacgc tatgaataat
     4921 ttcagcaatt tcttcaattt gcgtttcaaa aagccctaag gtactgggat tagtaatcat
     4981 aattcctgca gtattttcat ttactaattc gcgaagttta gctatgtcac agcgtccttc
     5041 tttattggaa gcgagttcca ctgttttata acctaccaaa ctacaggatg cgggattagt
     5101 tccatgtgcg ctatcgggaa taatgattgt atctttatga ttattaccct ttgctttatg
     5161 ataagcagca ataattttaa taccagtaaa ttcaccttga gcacctgctg agggttgaag
     5221 tgtaactcct ttcattccag atatttcggc taagtcattt tgcaattcat acatcagttc
     5281 caaagaacct tgcattgttt cttctggttg aaaaggatgc aaggtagtaa aagatgaatg
     5341 ccttaccagg gtttcattta gtttgggatt atatttcatt gtacagctac ctaatggata
     5401 taatcccttt tcgataaagt ggtttttatg cgaaagagat atataatgcc gcataacatc
     5461 caattcactc acttccggaa gacgcgcttt cttagttcta agcattgttt caggaatcaa
     5521 ggttggcaaa agtgtatcta tttctctttt gggtaaagtt actccttttc tacttggaga
     5581 gctatgttca aaaattgttt tactcatttt actacctcct tcatcgcctg aacaaaactg
     5641 tcaatttctg ttttgctctt cttttcggtt atcgctatca tcagctcatt cggttttccg
     5701 taaggagcaa gatcaattcc ggcatatata ttttcaggta gtaaagcgtt tataatatct
     5761 gcggcttttt tcggagtgcg gattacaaat tctttaaaga aagttgcttt gggataagca
     5821 agagccaaac tcggtatttt acaaagttcg cttgccgcat aatgtgcttt ttgaacagat
     5881 tgagtagcta cttcaataag cccttcttta cccaataaac acaaatatac tacggcagcc
     5941 aaagtgcaaa gtgattcatt ggaacatata ttggaagttg cttttgctcg tctgatatgt
     6001 tgttccctgg cttgtaaagt taaaacataa gctttttcac ctgctttgtc tattgttcca
     6061 cctacaattc taccaggcat caaacgcgcc atatccaatt tggtagcaaa gaatccaaaa
     6121 agcggacctc ccatatacat attattacca agtgcctgac cttcaccaac tgctatatca
     6181 gcattatatt ctgaaggggg tataagaaca gctaaagaaa caggatcaac actggcaatt
     6241 agtaagcact tggagttctt atgaacaata gtatctatag ctttggcatc ttccaagttg
     6301 ccataaaaat tgggagtctg gataactaca cttccaattg tttcatccat cattgcttcc
     6361 agggcagata aatctgtcac tccctctttt tcgggcacag ttaaaagctc tataccgata
     6421 ccttcagtat atgctttaat cactttaaca taattcgggt gtaaagttgc tggcagaatt
     6481 gctttagtta aatggttttt acgcacagcc atcaaaatag cttccgcaat tgaacttgct
     6541 gcatcataca ttccagcatt ggcaatctcc ataccagtaa gctcacatat cattgtttgg
     6601 tattcaaata tgtactgaag tgtaccttga cttacttctg cctggtaagg agtgtaggca
     6661 gtaaaaaatt ccggacgcga aactatggca tccactgcag caggaataaa atgatcgtaa
     6721 acacccccgc ccaaaaaaga atttacttta atacaaggtt tatttttatc tgcctttgtt
     6781 tggatattac ggtttatttc cagttccgat aggggttcgg atatagcaag aggattacac
     6841 attctcagtt tggcaggaat agcagagatt aaatcctcaa acttattagc gccgattacc
     6901 tttagaattt ttcgcctatc attatcggta ttagagatat agggcatttt cgctccttgt
     6961 tttatagaca tttgctttag tttatacttc aaaaataaag ttatggttta tgtcaattgg
     7021 tttttggatt tgcatagaat agcacagata tttatggcat taaactgatt cacactgata
     7081 atgtaagaaa aaggccttat gcactgatgg tattttcgaa acaaggatgt aaggattaga
     7141 ttttctgaaa caaggattta aggattttag gattaagagg atatagcttt ataaacaagg
     7201 atgttagatg agtatttagc caccgaaacc accgaaaata actcacacag gttcattttt
     7261 agaaacaagg atgtaaggat tagattttct gaaacaagga tttaaggatt ttaggattaa
     7321 gaggatatag ctttataaac aaggatgtta gaagagtatt tagctaccga aatcaccgaa
     7381 aataactcac gcagattcat ttgtagaaac aaggatgtta ggatttgaga agattatatt
     7441 ttataactga caattagtcg actttaagtc aaaatcggcc ttttctatga gattaatagt
     7501 gccctttttc aaagataggg aaaaagaatg aaattgtgtg gaaaaccaca aacaaaaccg
     7561 attttaactc aaaggtcaac acagtgttag cgatggtgcg taagcccctc gataaaaaag
     7621 ataataggca taggatgaac aggattttgg ggaattttag agcttgaatt attatattga
     7681 gaaatagtta agagaggaat cctctatgcc cttataattt tgttctttac gagaactttt
     7741 ttataggcgc agagaactcc tcactatggg aaatttattt ctttaatctg ataatcttaa
     7801 aagctttgta gtagccgtcc tatttccgga tactaaacgg caaagatata atccgctacc
     7861 aacatttttc ccttgtgtat cagttccgtt ccattccatt tcataatttc caactgtttt
     7921 attttccgta agcaggtttt ttactttctg acctttgagg ttgtagatag ttaaattcac
     7981 ttttccactt gcaggtagcg agtacttaat ttttgttgta gaagtaaaag gattgggata
     8041 gcttgataag gatatattca gggctgggca attaggttct tcgatactca cattaggatt
     8101 ttgtaaaagt agttcaatgg cagcagcgcc aacaattcct cttcccaaag catctgtcca
     8161 aggtctgttg ggttcataaa tccagctgtg accacaagca gcatttcgat caaagcgcat
     8221 attttgatat tcatagaggt ctaaaattac agcaatctgt cctgatatgt gggtcgtatc
     8281 caccacatta aaagtaaaat taggatcata atcaaaatta tagcaatagc caatatctaa
     8341 tagaagttga tcagtaattg ctccattgga aaatctatta atgcgggcat ataccattcc
     8401 aggcatatca ggttcacccc aaggatcggg tgtgatatta aacctaagcg cttttacaat
     8461 atattcttga ttccccaaat ccatatctat agcagctttt attgggatag ctccccgtcc
     8521 atattctatc caatgtggct cagaagaaag ccaggaaaac caattttcca gatcgggatt
     8581 aactatccga taattaatat taactgttgt agacaaattg gaagcagaaa aagcagttat
     8641 tccaatatta tggactccgg gggttaatag ataatcgctt attatacctg tatatggttc
     8701 agcagttgct gtataaacta aaatgccatc caaagtatat tccacacgct gtatagcctc
     8761 cgcaggatct gtaacttgaa gataaatatt cagcgtatta tcagctaaaa tagaaccatt
     8821 ggcaggatgg atagcgtcaa taacaggagc catagttact gctggagaga atattgtaaa
     8881 acttatagtt tcacctgctt cagtaatatt agtaatatta accggaaaag tagaaccgtt
     8941 atttagaaaa gaagatggat tcgtgtattc attaaaagca gttctaaaat tattagcact
     9001 gaatggggct tcagcaacca agccattatt ggttaaagat ccatcaggtc tataaacata
     9061 cactccatcc ggaggaccct ctacattacc atccattgtt tgatcaattc gataaatcaa
     9121 aagcccagaa ttgggtaagt tctcttcaaa aatatcgcta ctttttttcc ggtattcaaa
     9181 aattaaaaac tgattggttg aaccaggtat agggtattta tatacattat tttcagcaga
     9241 agtaactgga tttagtgtat aggtattacc aatttgagca agaggaatag aaggtatcca
     9301 tcccccatag gcatatttca tatacatccc catatgccca tctccacttt ccattaagtc
     9361 ccaacaacca accggagtta aaccgtcata ttcataatga tataaatctg gagcaccaac
     9421 actatgaaac atttcatgac aaagagtagt tacatcattt tggtcttccg gttgaaaagt
     9481 aaaatcataa acctctttat tatttatata ggcactggta taaaacagag accaacggtg
     9541 tgcccataaa agatctgccc aagcagaatg tggaccccga attataaaac agacattatc
     9601 cacagtacca ttgttatccg catcaatatt taaagaagaa ggaacttgcg aagcaataga
     9661 atttatggca ttcataagca aggtctgttc acggatacat ctttcatctt cattatgata
     9721 accaccagga tttgtaatat aattatatgg aagataataa ctacggggat ggctatcctg
     9781 atatgaaaga ttaaaaatat ctgggcatac gggataatga taagtcatat aatgcaactg
     9841 attataactt gctttttgaa aatagctccg taaactgcga gcatcatcgc caacctcatt
     9901 aaaacgggca tcataaactg tgcgcggaat ctcgaattcg gtctgatcgc tgaaacggat
     9961 atatacgcat agattgttta ctacaccagt gctagggcct cgttcattcc ctcttttatg
    10021 agcattcata aagttaattt ttctctgcct ggctcctttg gaaatattaa tattgggagt
    10081 tataccataa gaagcaggat caattgttcc aaaacgataa gctgaaggaa ccggttcacc
    10141 gttttccaag attgcataat aataatatcc atcatccgga ctttgaatta ttgtaaaacc
    10201 attagcatcg tgtaaacgat tagaatattc atcaccactg gcatataagt ttagaacaga
    10261 gccatcaggt tgaatcgctt gtgaaggtat attataaaaa ggggcagcaa atcccaaaat
    10321 agggataata cataataaca agcaagtaca tttcttcata attcatctcc ttatatataa
    10381 atagtaagca agatctgtac ctaaagcaat caaaaaagca taaaaacaat atgttaaaac
    10441 tgttcagtcc taaaatagca gactgaattt tgctttttcc tgttaaaaaa accaacgaaa
    10501 atttgcagat aaagaccatc gaacgacagg atgtcgttct tcccgaaaat aactcacgca
    10561 gatttagttt tcgaaacaag gattttagga ttttaggatt aagagaattt atctctataa
    10621 acaagatttt ttagaagaat atttagccac cgaagccacc aaaaataact caccaaagat
    10681 tactcaaatg ttagcaatct caagttcgaa gaaatcacta tcctgaatat gcaacctttc
    10741 aataaacttt ttgtgcattt cttacttctt gggttaagtc cactttttcc aggatgcctc
    10801 actttttgcc tctcctcatc cacctatcct tctctcctcc tatactcctc gattaggagg
    10861 agagaggaac gagtagagaa agagtatcgt ccaagataat atcaagatga caattaa
//
LOCUS       DAOSTH010000053        10808 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_5967_length_10808_cov_13.835581, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000053 DAOSTH010000000
VERSION     DAOSTH010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10808
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5967_length_10808_cov_13.835581"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PLB65_06360"
     CDS             <1..551
                     /locus_tag="PLB65_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOQ78187.1"
                     /translation="TPDQRRAAKTINFGILYGMGQRKLSRELGIPQNEAKQIIDDYYA
                     QFPSIREFINQCILKARQQHYAETLFGRRLYLPNIESKNQGLRSEAERIAVNMPIQGT
                     AADLIKIAMLDIHNQIDKREDIKMILQVHDELLFEVHQKAIEEAKIIVQTCMEHALPK
                     EYADKVHLKTDLGVGVNWFEAH"
     gene            complement(651..1376)
                     /locus_tag="PLB65_06365"
     CDS             complement(651..1376)
                     /locus_tag="PLB65_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78188.1"
                     /translation="MKRLIMLLLTLLVLAGCVQYDEELWINRNGSGRAVIRIVHRSPY
                     ENPEEIMRKAALPGINLQSYDIYRKGPDVIYTINFKFKNIDAFNNVNDQLGTADFWGK
                     ITLNKEPGRRITFKRRIALGSQEPEDEDDIEHIIGRLQPDNPVWTYKVHLPWKIISTN
                     ALPENVDYNNRTVAWKYDTRKMWHKQELMSVELRKDFPWLLLVIGVIVLILLTFLVHW
                     ILRFPKKSHWLERIKHTEDAEKK"
     gene            1518..1895
                     /locus_tag="PLB65_06370"
     CDS             1518..1895
                     /locus_tag="PLB65_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin"
                     /protein_id="HOQ78189.1"
                     /translation="MIALRQLWRCAICGNVVEVTYVGGGELVCCGQPMYLLQENTVDA
                     ATEKHLPVVKDLGDKIEVSVGSVPHPMEEKHYIVFIEVLTDKKVLRKELKPGEEPKAI
                     FKVSMDKVKEVREYCNIHGLWKA"
     gene            1916..2506
                     /locus_tag="PLB65_06375"
     CDS             1916..2506
                     /locus_tag="PLB65_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HOQ78190.1"
                     /translation="MSFRETRTAENLMKSFAGESQARMRYLYSAKTAKKEGFEQISNI
                     FTETADNEKEHAKVFFKHLLSNGVEGEMINIIASYPVGWSADNTLKNLEYAANGENEE
                     WSELYPLFADVAEEEGFSDIAHSWRMIAKVEREHEKRFRKLYDNVKNQKVFIRQEKVL
                     WKCLNCGYVHEGTEAPKICPSCSHPQSYFELFKENY"
     gene            2527..2667
                     /locus_tag="PLB65_06380"
     CDS             2527..2667
                     /locus_tag="PLB65_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HOQ78191.1"
                     /translation="MQKYQCMACGWIYDPAENNNIPFEELPDDFVCPECGVGKDIFEP
                     YE"
     gene            2747..3199
                     /locus_tag="PLB65_06385"
     CDS             2747..3199
                     /locus_tag="PLB65_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HOQ78192.1"
                     /translation="MQQQEFEEILDFAISREEEAVKFYQELQEQSQFQDVKNMLKDLE
                     MMENGHIQIIENIRITGVKEEDIQKVPNLKISEYLCVDPSDLDMSYQNLLVRAMKREE
                     NSFLLYSEMSVKFPDAEIATLFRRLAADEAKHKLFFENLYDDWIRTGN"
     gene            3196..3870
                     /gene="tsaB"
                     /locus_tag="PLB65_06390"
     CDS             3196..3870
                     /gene="tsaB"
                     /locus_tag="PLB65_06390"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOQ78193.1"
                     /translation="MKLAIDSTQNSGSIALANEERVIYSAYFDIKITHSETLMPAIDY
                     AFKFCNILRSELQEIYVCIGPGSFTGLRIGLATAKGIAFALGIPLYAYSSLELAALPA
                     SGLGKNIFAAVDAKMQEVYYAYYDQQLKEIIPPSIATPEELCNLDLEDFILCGSASEM
                     LALRFKNSYNFYTLHPVMKIPSAAGLFSIPNLLPEKHFPQILEELEPLYLREARAQVK
                     KAKPKQ"
     gene            4242..7862
                     /locus_tag="PLB65_06395"
     CDS             4242..7862
                     /locus_tag="PLB65_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ78194.1"
                     /translation="MKKAFILLTAILIGINLAAFETVIKVDKNAWQTKQQADGYPVTL
                     QTGEPVLPYVPVNVLIPFGQRYQNASIQLAPAELVAERVTFEIAGVQLPISRMNIPNA
                     GSYSSAVLENKLYPEREWQFLGIQYFRGYQIALFNVYPYRYNPITQKLYVSSEISVSI
                     NSEFSEAEAEYQANFYTPNSKTLSYINSLICNPENIASYQSASNYRSVPILSRDIDLT
                     VPKQMIIITSNSRINWFTEYSSWRASKGVSNAIYALEDILSSYPGTDNAEKLRNFIIN
                     VYQTWANSSQPLEYVILGGDDEIIPERGTYGQVGDTIDARMPTDIYFSNLDGNWNANN
                     NNIWGEPADNPDMIPEVHIGRFPAETQNEFNHMFNKIRYYVDNDTYSNNYALFMGENL
                     NNNPLTWGGDYKDDVAQYLPDEYYLQTLYQRDGTYSSMNVYNAINKGAGIMNHMGHAN
                     ESVLIGQSNGTVESLTNTEYGFLYTQGCYPAAFDQRTSGDNESIGEHFVTASGGVFCF
                     IGNTRYGWYSPGSVNGASQFYDRQFFNGLFQQNLPVLGEALTYSRLQNLNAALSNDVM
                     RWCYYEVVLFGDPSIAVKSFNPNLPLLSLESYEITDEEGDNNGTISTGELICIYPVIS
                     NNTNWGTAYNVSVRLENLPEGIEVVGNNLSIAQIIPGGMSPQNLKIRLQLSDTVGFGT
                     YYLTLVIDSINPVTNLSTGERRFTVSFEITLMDDRFPWETIINGKSAPLVTNLNSTSG
                     MEIIYVDVYGNSYYLGNDGEIYSTVEAPEGMYIFRSFACGPIDDDDENDLVFCSRSGD
                     IYAMHFDGEIIFNYHDNTMFLFTPVLADINGDGTLETIAGSLFGKIYAISPNGQLLNG
                     FPFDVGGNFQTELAAADFNGDGTMEIVAGSQNGLLYVLGTGGTVLPGFPVQLNGPVTG
                     SPTITNTNRIVCSTPTHIYIISAEGNIIAMRNIDANIAGGFALGDITSDDNGLDIVGI
                     TNNGIIYALTDEGVDLPGYPVATGDNFVCPPLIANLDSDPQAEILVHSYVNSVYAFNS
                     DGSTLNGFPFANTYNGSTPATLVDFDNNNQIKLIMGHSNGVLMLNLRRDASELKPWIT
                     YRGSTLRQGSFASTGFVSNAEETNVPVMNALLGNYPNPFNPETTICYTTKAEAKARLE
                     IFNIKGQKIRTLVDTVLKEGNHSIVWNGRDDYSREVASGVYFYRLSIEGKSYQHKMLL
                     LK"
     gene            7859..8560
                     /locus_tag="PLB65_06400"
     CDS             7859..8560
                     /locus_tag="PLB65_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOQ78195.1"
                     /translation="MKIAENIKYIQNNIAETLSKMGRKDEVTLVAVTKTHPVEVIEIA
                     LQLGIEHIGENKVQEARRKIPMLKVPYQGFHFIGHLQSNKINQLLELKPCLIQSIHSL
                     DLAQKLHLALGRTNRSQDILIQINSSSELSKSGFSFEEVKEAIWQIAAYPTLHIKGLM
                     TIGMLAEAEVSRPLFAKMKYLFEEIKELTIPNVEMKYLSMGMSNDYVQALEEGSNMLR
                     IGTGIFGERSYGANE"
     gene            8557..9204
                     /locus_tag="PLB65_06405"
     CDS             8557..9204
                     /locus_tag="PLB65_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HOQ78196.1"
                     /translation="MIYLFVILVIIVAYLYGCFSTARIVAKGFRSLNVYKIGTGLADT
                     ENIYTNISKPLGTLVGAIDVAKAYLFLLIVEVLLRFIDTSVINYPGFSVLYHENLMLV
                     YGMSMLIGHCLPYNHHFRGGRGIFTYMGMLAYFAFLPTCISALIAWVIVVRFKQIRFA
                     QYLIVILPVVLFIIFYTLIPNYHKDLPSYFITLLLGNAVIMGILNIIVSKRLGEF"
     gene            9226..10353
                     /locus_tag="PLB65_06410"
     CDS             9226..10353
                     /locus_tag="PLB65_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ78197.1"
                     /translation="MIDVIPVDNKKQLKEFIMLPFELYKNDSNWVPPLISDQKKFFNP
                     KKNPYYQHSEVKLFLAYKDGKQSGRISAHSNTQHNIEHNDNIGFFGFFECIDDQEVAD
                     ALFEAAYKWNKYRGFISLRGPLNFSVNHDCGLLIKGFDVPPMVMMPYNFSYYQKLYEN
                     YGLTKTMDLYAFYNEYHSIPERITKMADIVSKRNKVEVRSLSYDKKQRKKDIETVFEI
                     YTKAWQYNWGNVPMTKAEFDNIVAELLPIADPDLVFIAEVDGKLAGFSLALPNYNEVL
                     KVMKGKVTPLTVIRALKAKKHINAVRVITMGIVKEFQGRGIDALLYYHTFKKGLPKGY
                     YRSEFSWVLENNTPMINVAEKLGADIYKTYRLYDKAIEDRI"
     gene            10364..>10808
                     /locus_tag="PLB65_06415"
     CDS             10364..>10808
                     /locus_tag="PLB65_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78198.1"
                     /translation="MTEQNIREPRRIISFLSAIDILTLVYCGWIILYMTFGFTRSPEA
                     IKHIPVYLAIFTGVLFLAWLQKQSGWCYSPNNPTKRYRVLNFFRSIYPVLLFGYFYTS
                     GYAFNRIVFRNWLDPFFMSIDKYIFGYLPSLVWGKLYTHWAVQELF"
BASE COUNT         3480 a         1779 c         2127 g         3422 t
ORIGIN      
        1 ttactccaga tcaaagaaga gcagccaaga ctattaactt tggcattctc tacggtatgg
       61 ggcaaagaaa actttcccgc gaactgggta tccctcaaaa tgaagcaaaa caaataattg
      121 acgactatta tgcgcaattt ccttccatcc gagagtttat taatcaatgt attcttaaag
      181 cccgccagca acattatgct gaaacccttt ttggtaggcg tttatatctt cctaatatag
      241 aaagtaaaaa ccagggattg agaagtgaag cagaaagaat tgctgtaaat atgcctattc
      301 agggaacagc agctgattta atcaaaatag ctatgctgga tattcataat cagatagata
      361 aacgggaaga tataaaaatg attctacaag ttcacgatga attgctcttt gaagttcatc
      421 aaaaggcaat agaagaagct aaaataatag ttcaaacctg tatggaacat gcgttaccta
      481 aagaatatgc ggataaagtt cacctgaaaa cagacctggg agtgggagta aattggtttg
      541 aagcgcactg agaatgaaga attaagtcag aagctggcat cgcttatata actttaaatc
      601 agccatataa ccaaatttgt ggaagctgca taaacattat ctgtaggata ctatttcttt
      661 tctgcatcct cggtatgttt tattctttct aaccaatgtg atttctttgg aaagcgtaaa
      721 atccaatgta ctaggaaagt gagtagaata agaacaatta ccccaataac gagtagcaac
      781 caagggaagt cctttcttaa ttccacagac ataagttctt gcttatgcca cattttacgg
      841 gtatcatatt tccaggcaac ggtacgatta ttataatcta cattttccgg taaggcattg
      901 gtagaaataa ttttccaagg tagatgaact ttatatgtcc atacaggatt atcgggttgt
      961 aatctaccaa taatatgttc aatatcatct tcgtcttcag gttcctgact gccaagagca
     1021 attcttcttt taaaagttat tcttcttccg ggttctttat tcagagttat ttttccccaa
     1081 aaatcagctg ttcctaattg atcattcaca ttattaaaag catctatgtt tttgaattta
     1141 aagttaatag tataaataac atcaggacct tttcggtaaa tgtcatagga ttgtaaattt
     1201 attcctggta aagctgcttt gcgcataatt tcttctgggt tttcataagg agaacgatga
     1261 actatacgga taacagctct tcctgagcca ttgcgattaa tccatagttc ttcatcatat
     1321 tgaacgcatc cagccaaaac cagaagcgtt agcagcagca ttataaggcg tttcatttag
     1381 caatccttct ttattaataa agctttataa atcaataaaa caaaaagcgt tttttttggc
     1441 aagggaaaaa tttcagttga caaaagtact aaattgctta taataaaaga aagagaatca
     1501 attagggagg aaagaaaatg atagcattaa gacaactatg gcgttgcgct atttgtggaa
     1561 atgtagttga agtaacttat gttggaggtg gtgaattagt ttgctgcggt caaccaatgt
     1621 acttgttaca agaaaacaca gtagatgctg caacagaaaa acatttacct gtcgtgaaag
     1681 atttgggaga caaaattgaa gtctctgtag gaagcgttcc tcatccgatg gaagagaaac
     1741 attatattgt atttattgag gttctaaccg ataagaaagt tttgcgcaaa gaactgaaac
     1801 ctggagaaga acctaaagct atttttaaag tatcgatgga taaggttaaa gaagtgcgtg
     1861 aatattgtaa tatacacgga ctttggaaag cataactata ataaggagaa gaaaaatgtc
     1921 atttagagaa acaagaactg ctgaaaatct gatgaaatct ttcgcaggtg aaagccaagc
     1981 cagaatgaga tatttatatt cagctaaaac agctaagaaa gaaggctttg agcaaatatc
     2041 taatattttt acagaaactg cagataacga aaaagaacat gccaaggtat tcttcaaaca
     2101 tctattgagt aatggtgtgg aaggtgaaat gattaatatc attgcttctt atcctgttgg
     2161 ttggtctgca gataacacac taaaaaatct ggaatatgca gcaaatggtg aaaatgaaga
     2221 atggtcggaa ttatatcctt tgtttgccga tgttgcagaa gaagaaggtt ttagcgatat
     2281 agctcattcc tggagaatga tagctaaagt ggaaagagaa cacgaaaagc gttttcgcaa
     2341 gctctatgac aatgtgaaaa accagaaggt ctttattcgc caggaaaaag tcctatggaa
     2401 atgtttgaat tgtggatatg tgcatgaagg aactgaagct cctaagattt gcccttcctg
     2461 cagtcatcct caaagttatt ttgaactctt taaggaaaac tactaaaaac tgcaaggaga
     2521 ccaaaaatgc aaaaatatca atgtatggca tgtggatgga tttacgatcc cgctgaaaac
     2581 aacaacattc ccttcgaaga gcttcctgat gattttgtct gcccagaatg tggtgtcgga
     2641 aaagatattt tcgaacccta cgaataacta aataaccttt ctggccactg gggagtttta
     2701 tctctccagt ggcttaacaa aaaataaatg atatgtgagg tctaaagtgc aacaacagga
     2761 atttgaagaa attttggatt ttgctatctc gcgtgaagag gaagcggtaa aattctatca
     2821 agaattgcaa gaacaatccc aatttcagga tgtaaagaat atgctgaagg atttggaaat
     2881 gatggaaaat ggtcatattc agataattga aaacattcgt ataacaggtg tgaaagaaga
     2941 agatattcaa aaagtgccca atttgaaaat aagtgaatac ttatgcgtag atccctctga
     3001 tctggatatg agctaccaaa atttacttgt tcgagctatg aaacgagaag aaaactcctt
     3061 tctgctctat tcggaaatga gcgttaaatt tcccgatgca gaaatagcta ctctttttag
     3121 acgcctggct gccgatgagg caaagcataa actgtttttt gaaaacttat acgacgattg
     3181 gatcaggact ggaaattgaa acttgccata gattctactc aaaattccgg ttctatagct
     3241 ttagcaaatg aagagcgggt aatctattct gcctattttg atataaagat aacccacagt
     3301 gaaacattga tgccagcaat tgactacgca ttcaaatttt gtaatatttt gcgtagtgaa
     3361 ttgcaagaaa tctatgtgtg cataggtcct ggttcattta ccggactaag aatcggattg
     3421 gcaaccgcaa aagggattgc cttcgcttta ggaattcccc tctatgcata ttcctctttg
     3481 gaattggcag cattaccagc aagcggattg ggaaaaaata ttttcgctgc cgttgatgct
     3541 aaaatgcaag aagtttatta tgcctattac gatcagcagc taaaagaaat tatacctccc
     3601 tccattgcca cgcctgaaga attatgtaat ctggatttgg aggattttat cttatgcgga
     3661 agcgcttcag aaatgcttgc tctgcggttt aaaaattcct ataattttta tactctccat
     3721 cctgttatga aaattccttc ggcagcaggg ctgttttcta taccaaactt gttaccggaa
     3781 aagcacttcc cccaaatttt ggaagaattg gaacccttat atttaagaga ggcaagggcg
     3841 caggtaaaaa aagcaaaacc aaaacagtga aaaaaataag aaaaaatgca cttgttacac
     3901 aacaatctcc tgtttactcg gttgctgcat acataattta acgatagcag cattctttta
     3961 tgggaaccag gattgtttct tccttagtta tttgtggtag agttgcggtg gagctacggc
     4021 gggaaaatgc cgagtaacac cattgtagct cagcggtagt taataagtag gaaccaagca
     4081 aaaatgaata agttagtttc aattgaggaa gtaaacatag gatcaaatta tttttcagat
     4141 aaccctcggg aaataacttg acggattggt tgattttatg ataaaggaac agaacttgct
     4201 gtaattatct tagcagaaca aaaaaaaaga tggatatgaa tatgaaaaaa gcttttatat
     4261 tactaacggc catcctgatt ggaatcaatt tggctgcctt cgaaacagtg attaaagtag
     4321 ataagaatgc ctggcaaacg aaacaacagg cagatggtta tcctgttaca ttacaaactg
     4381 gtgagccggt tcttccttat gtaccggtaa atgtactaat accttttggg caaagatatc
     4441 aaaatgccag tattcaatta gctccagctg aacttgtggc agaaagagta acttttgaaa
     4501 ttgctggagt tcaattgcct atttccagaa tgaatattcc caatgccggt tcttattcgt
     4561 cagcggtttt ggagaataag ctttatcctg aaagggagtg gcagttttta ggaattcaat
     4621 attttcgtgg ttatcaaatt gcgcttttca atgtttatcc ttatcgctat aatcctatta
     4681 cgcagaagct gtatgttagt tctgagatta gtgtatccat caacagtgaa ttttcggaag
     4741 cagaagcaga atatcaagcg aatttttaca ctcctaattc taaaactcta tcttatataa
     4801 attcgctgat ttgcaatccc gaaaatatag caagctatca atctgcaagt aattatagaa
     4861 gtgtacccat tttaagccgg gatattgatc ttacggttcc caaacaaatg attattatta
     4921 ctagtaattc ccgaatcaat tggtttactg aatattcttc ttggcgtgcc agtaaaggtg
     4981 tttccaatgc tatttatgct ctggaagata ttcttagttc ctatcccgga accgataatg
     5041 cagaaaagct tcgtaatttt attataaatg tttatcagac ctgggcaaat agttctcaac
     5101 ccttggaata tgttattctg ggcggtgatg atgaaattat tccagagcga ggcacttatg
     5161 gccaagttgg ggatactata gacgcaagaa tgcctacgga tatttatttt agtaatttag
     5221 acggtaactg gaatgctaac aataataata tttggggtga accagctgat aatccagata
     5281 tgattccaga ggttcatatt ggtagatttc ctgctgaaac tcaaaatgaa tttaaccata
     5341 tgtttaataa aatccgctat tatgttgata acgatactta tagcaataat tatgcgttat
     5401 ttatgggcga aaacctgaat aacaatcctt taacttgggg aggtgattat aaagatgatg
     5461 tggcacaata ccttcctgat gaatattatt tgcaaactct ataccaaagg gatggcactt
     5521 atagctctat gaatgtttat aatgcgatta acaaaggcgc tggaattatg aatcatatgg
     5581 ggcatgcaaa tgaatcagtt ttaattggtc aatccaatgg aaccgtagaa agtttaacta
     5641 atacagaata cggttttcta tatactcagg gttgttatcc tgctgctttt gatcaacgta
     5701 cttctggcga taatgaatca atcggagaac attttgttac ggcttctgga ggagtatttt
     5761 gttttattgg caatactcgt tatggatggt atagtcctgg aagtgttaat ggtgcttctc
     5821 aattttatga tcgtcaattt tttaatggtc tatttcaaca gaatcttcct gttctgggag
     5881 aggctttaac ctattcccgt ctgcaaaatt tgaatgcagc attaagtaat gatgtaatgc
     5941 ggtggtgtta ttacgaagta gttctttttg gtgatccttc catagcagta aaatctttta
     6001 atcctaattt gcctttgctt agtttggaga gttatgaaat taccgatgaa gaaggtgata
     6061 ataacggaac aatcagcact ggcgaactaa tttgtattta tcctgttatc tctaataaca
     6121 cgaattgggg tacagcatat aatgtatcag tgagattaga aaatttacct gaaggcattg
     6181 aagtggtagg taacaattta tctattgctc aaattattcc tggtgggatg agccctcaaa
     6241 atttgaaaat tcgtttacaa ctatctgata ctgttggttt tggcacttac taccttaccc
     6301 tggttattga ttctataaat cctgtaacta atttatcaac tggtgaacgg agatttactg
     6361 ttagttttga gattacttta atggatgatc gttttccttg ggaaactatt ataaatggaa
     6421 aatcagcacc tttggtaaca aatttgaatt ctaccagtgg aatggaaatt atctatgttg
     6481 atgtgtatgg gaatagttat taccttggta acgatggtga aatttattct acagtagaag
     6541 cacctgaagg tatgtatata ttccgcagct ttgcttgtgg acctatagat gatgatgatg
     6601 aaaatgatct ggttttttgt agccgtagcg gtgatattta tgctatgcat tttgatggtg
     6661 agataatctt caattaccat gataatacaa tgtttttatt cactcctgta ctggcagata
     6721 taaatggaga tggaacttta gaaacaattg ccggaagctt atttggaaaa atctatgcca
     6781 tttcccccaa tggacaattg cttaatggct tcccgtttga tgttggcggc aatttccaaa
     6841 ctgaacttgc tgctgccgat tttaatggtg atggaactat ggaaattgtt gctggttccc
     6901 aaaatggact tttatatgtt ttaggaacag gtggaacagt tcttcccggt tttcctgttc
     6961 agctaaatgg tcctgtaact ggttctccta cgattacaaa tacaaataga atagtatgtt
     7021 ccactcctac ccatatctat attatttctg cggaaggaaa tatcatcgcg atgcgtaata
     7081 ttgatgctaa tatagccggt ggttttgctt tgggagatat tacttctgat gataatggat
     7141 tggatattgt tggaataaca aacaacggga ttatttatgc cttaacagat gaaggtgttg
     7201 atcttcctgg ttatcctgta gcaactggag ataattttgt ttgccctcct cttattgcta
     7261 atttggatag tgatcctcaa gccgaaattt tagtgcatag ttatgtaaat tctgtgtatg
     7321 cgtttaattc tgatggcagc actctaaacg gatttccttt tgccaatact tataatggta
     7381 gcacgccggc aactttagta gattttgata acaacaatca gataaaatta attatgggac
     7441 attccaacgg cgttttgatg ctcaatttac gaagagatgc ttccgaatta aaaccttgga
     7501 ttacttatag aggaagtact ttaagacaag gttcctttgc ctcaactggt tttgtaagta
     7561 atgctgaaga aacaaatgtg ccagtaatga atgcactgct gggaaattat cccaatccct
     7621 ttaacccaga aactacaatt tgctatacta caaaagcaga ggctaaagct aggctggaaa
     7681 tattcaatat aaagggtcaa aaaatacgaa ctttggtaga taccgtcctc aaagaaggca
     7741 atcatagcat cgtttggaat ggaagagatg attattccag agaagtagcc agcggtgttt
     7801 atttctatcg tttgagtata gagggcaaaa gctaccaaca taaaatgtta ctattaaaat
     7861 gaagatagct gaaaatataa agtatatcca aaacaatatt gctgaaaccc tttccaaaat
     7921 gggaagaaag gatgaggtaa ctttggtggc tgtaaccaaa actcatcctg tagaagtaat
     7981 agaaattgct ttacaattgg gaattgaaca tattggcgaa aataaagtgc aggaagcaag
     8041 aagaaaaata ccgatgctaa aagttcccta tcagggtttt cactttatcg gccatttaca
     8101 aagtaataaa ataaaccagc tgctggaatt gaaaccttgt ttgattcaat ctatccactc
     8161 tttggaccta gcccaaaaac tacacttggc tttagggagg acaaatcgca gtcaggatat
     8221 tttaattcaa ataaatagca gcagcgaact ctcgaaaagc ggtttttctt ttgaggaagt
     8281 aaaggaagca atttggcaaa ttgctgcata tcctactttg catataaaag gtctgatgac
     8341 tataggtatg ctagcagaag cagaagttag ccgtcctctt tttgctaaaa tgaaatacct
     8401 gtttgaggag ataaaagaac ttactattcc taatgtggaa atgaaatatc tctctatggg
     8461 aatgagtaat gactatgttc aagctctgga agagggttct aatatgcttc gtattggaac
     8521 cggcattttt ggcgaacgaa gttatggagc taatgaatga tttacctatt tgtaatatta
     8581 gtaataatag ttgcctatct ttatggctgt ttttccactg cccggatagt tgcaaaaggt
     8641 tttcgctctt taaatgtata taaaatcggc acaggtttgg ctgatacaga aaatatttac
     8701 actaatatca gtaaacctct ggggacatta gtaggagcaa ttgatgtggc taaggcttat
     8761 ttatttttgc tgatagtgga agtgctttta cggtttattg ataccagcgt gatcaattat
     8821 ccaggattta gtgttctcta tcatgaaaat ttaatgctgg tttatggaat gagtatgctt
     8881 atagggcatt gtttacccta taatcatcat tttagaggtg gacgcggtat ttttacctat
     8941 atggggatgc ttgcctattt cgcttttttg cctacttgta tttctgctct tattgcttgg
     9001 gttattgttg tccgttttaa gcagatacgg tttgcccaat acttaattgt gatcttgccg
     9061 gtagttttat tcattatatt ttatactctt atccccaatt accataaaga tttgccttca
     9121 tattttatca ctcttttact tggtaatgca gtaattatgg ggatattgaa cataattgta
     9181 tctaaaagat taggcgaatt ttagcttagc aaaagaggtt cagcgatgat agatgtaata
     9241 cctgttgata acaaaaagca gctgaaagaa ttcattatgc ttccctttga gctgtataaa
     9301 aatgattcca attgggttcc tcctcttatt agtgatcaga aaaagttctt caatcctaaa
     9361 aagaatccct attatcaaca ttcagaagtt aaactctttc ttgcttataa agatggaaaa
     9421 caatcgggac gaatttctgc tcattccaac actcaacata atatagaaca taacgataac
     9481 atcggattct ttggcttttt tgagtgtatt gatgatcagg aagtggctga tgctcttttt
     9541 gaagcagctt ataaatggaa taagtatcgt ggttttattt ctctgcgagg tcccttaaac
     9601 ttcagtgtaa atcatgattg cggtctgttg ataaagggtt ttgatgttcc tcctatggtt
     9661 atgatgccct ataatttctc ctattatcaa aaactctatg agaattatgg cttaactaaa
     9721 acgatggatc tctatgcttt ctataatgaa tatcattcta ttccagaacg cattactaaa
     9781 atggcagata tagtttccaa acggaacaaa gtggaagtcc gctctctttc ttacgataaa
     9841 aaacaacgaa aaaaagatat tgaaacagtc tttgaaatat acactaaagc ttggcaatat
     9901 aattggggaa atgttccaat gacgaaagct gaatttgata acatcgtggc agaattactt
     9961 cctatagccg atccggattt agtctttatt gctgaagtag atggaaaact tgctggtttt
    10021 agcttagcat taccaaacta taatgaagtt ctaaaagtta tgaaaggaaa ggtaactccc
    10081 ctaacagtta taagagcgct aaaggcaaaa aaacacatta atgccgtgcg cgttatcacc
    10141 atgggaatcg taaaagaatt tcagggacga ggtatagatg ctcttttata ttatcatact
    10201 tttaaaaaag gtcttcccaa gggttattac cgtagcgaat tttcttgggt gctggaaaat
    10261 aataccccta tgattaatgt agctgaaaag ctcggagcag atatctataa aacttaccgg
    10321 ctttacgata aagcaattga agacaggata taacggattc taaatgacag agcagaatat
    10381 tagagaaccc agaaggataa tttcatttct ttctgccata gacattctca ctctggtgta
    10441 ttgcgggtgg ataatactat atatgacttt tggttttaca cgctctccgg aagcgataaa
    10501 acacattcct gtttatctgg caatttttac aggagtactt tttttggctt ggttacaaaa
    10561 acaatccggc tggtgttatt ctccaaataa ccctactaag cgttatagag ttctaaattt
    10621 ttttagaagc atctatcctg tgctcttatt tggttatttt tatacatcag gatatgcttt
    10681 caaccggatt gtttttcgga actggctcga tccctttttt atgagtatag ataaatatat
    10741 ctttggttat ctcccttctc tggtttgggg aaagttatat acgcattggg ctgttcagga
    10801 actttttc
//
LOCUS       DAOSTH010000054        10727 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_6014_length_10727_cov_10.246346, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000054 DAOSTH010000000
VERSION     DAOSTH010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10727
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6014_length_10727_cov_10.246346"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(199..873)
                     /locus_tag="PLB65_06420"
     CDS             complement(199..873)
                     /locus_tag="PLB65_06420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ78199.1"
                     /translation="MSEISIYIIDDHKLFVEGLCSLLAEVEGFKIAGYSLNPLEFINL
                     DEQPKVNVYLMDINMPQISGVELTIRLKKINPEAKILALTMFDEYEYVEQMIVAGASG
                     YILKSESLQELIRAIKIVAEGLKYFGTEIQEIIFNKVEKSSASKNTKPWINDNKHVAT
                     LTKREIEILILISKEFSTRQIAEKLYISERTVETHRKNIFSKTKAKTAIGLSKYAEKH
                     GYITKS"
     gene            complement(886..2853)
                     /locus_tag="PLB65_06425"
     CDS             complement(886..2853)
                     /locus_tag="PLB65_06425"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ78200.1"
                     /translation="MKKSFLSFGLLILIFMLSQQIKSEIWLLSGNNKKNRECIKINGQ
                     PIDTLFLKLQNLVYHSPDSAQLLAKEALLIVEQINHIDWQVRLLNLVGTCYSIKSEYI
                     KALECYHKALKLAMDSDDNERLGDSYNNIGGVHFFARNYTEALTNYLEALTYYELDGA
                     YHKVAGVNCNLGVLYDALGNTEKANCHYKESFSGFEKLKIFPGQTLVLTHISQNYQLG
                     GKYDSALVVIDQAIRLADSIQEIYSLSLALKTKGDIYIALNDLDQALNYYKLSETEVK
                     RIQNKNTLSIIHIGFAKAYLKKGDIQQSENHALEALNMANDLDDEKLIVDCHMLLSKI
                     METKGNYHIALSHFKEGTDVNNKIMDKSKLHGIYNREIQHLSKDKEIQKLEIERQQYL
                     IKQRNTMIYIIVLIFLIIIVLIVSLYFLYANKVKVEQQKKINDANLKVAEERAKAALD
                     AEIQERKLLGLELHDRVGPLLSLVKLNITALAGQLDSINDRRAKMVNSTLDTINEILK
                     EIKQISQNMAPIILIEKGFEAAIKNLVVRLNETNNYQVNLDMYAINDKLEPYVEHVLY
                     RSILESINNVLSHAKGTEINIQIVGNQDDITVMIEDNGQGFDVSDLQKNNGLGMKSTQ
                     KRIEGFKGNMFVDSKIGKGTIVTFIIPSKTN"
     gene            3099..6002
                     /locus_tag="PLB65_06430"
     CDS             3099..6002
                     /locus_tag="PLB65_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOQ78201.1"
                     /translation="MKKLLLLTFLILTLNWVGLAQVGSSYNFTQTQLTYYPLTEDIVL
                     WSGSFQYETPIEIEIPDFPFDGTIYNSVFVSSNGFITFGSAPTVNNTKPISSDEDYSG
                     AVSAFSGNLTPAITGNPQISYKLDDDFFIVQWQDVRRYLVYEDIISFQIILDFNEGII
                     SVVYGGDIASGQNSAFPQVGLRGQTNNFPSHINNRMILINGGNWINSQPGIANNSTMY
                     FRNTSPETIPSPGLTFTWTPQSSAPLLISPVSHDFGEVLVSMTSDPFSFSLSNTGIMS
                     VTIDSAFITGENADEFQIVDLPTFPIVLDEFEVVNIKVTFSPTEVGLRNGKLIVNTDG
                     GDFEAELTGTGYEEGIFDLPFIEGWDSGNFTENNWSFINPTSTWRISPGTMGNPPPCA
                     QFHYSPRHYNYSTSLISPTLNISNTINWELSFDMKLSNYLNSGTEYFDVHMLLDSNWV
                     LIDSFVNFFDLEWTTYTYNLDTLQGDVTRIRFEMHGEDNDNLNNWSIDNIVFNGEIVY
                     LFELDPEHVDFGEVMTGTTSDPFIVTYANNGLNEIGVNSSVIIGDQSDYFILTDNNQY
                     PFILSPGQSADVSVQFAPLEEGNASASLVISTEVGTFTSTLGGFGYDEYIGTIPFFED
                     WATGSFKTRQWSFDQTETNWLIQNNSGNPAPTARFSFSPIIEGYSQSLISPRIDVPVS
                     NQKIALSYDLKLADYETSGTEWIKVYIWTGSEWQEVAAHSNNGNMAWQNFQYYITDYV
                     QNETRIRFEASGVNSANISNWEIDNISLDFLDLPVINVEPMALTQEIPYSGTAVQEVI
                     ISNEGTAVLNYSITINYIAPATGNWLSVLPTSGEVAVGNSQIVQVSFDTNGLTIGNVY
                     TAELIVENNDPDNPEVTISVTLTVLTSQNELVAKKIIIYPSPAKQIINLQGINEIILI
                     QIFNSSGQLIFGVENEGENIRQIDVSNLISGSYTMRFITTQNTYINHKLQIIK"
     gene            6024..8303
                     /locus_tag="PLB65_06435"
     CDS             6024..8303
                     /locus_tag="PLB65_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78202.1"
                     /translation="MKRVLIFSFLSLFVFFSVSRTEAQSWGNNNDYIVPISHSLANGI
                     MSQYPPTTSIVENNILYYTDLYGFHIYNVSNPQSPVEISSLPIPGRARHFTIKGNYAY
                     ILNELGVAFVNIENIYLPEIEDIAWIGFNPVRVIVDEECLFLAADDGVYAYSVSDTHQ
                     LSLLSYTYVAPAATTFAGFIKHDKYIYYANQVRLHVFDFEDPQNVIETSVSNFTGGGS
                     CWGGLAIKDDYLYVVTTLKLHIFNISNPSIPQIVYTGLPTSHTIYSIVVENNNLVLNH
                     SNNGKFTILDISNPTNPNVVYAYPGGWFHHLIYLGALKDNILYVLDNEQEVYNGFSLH
                     LIDINNVSSPTQITEIKSTPGRTRSVALLNKNNKSLALVAQDNESRSGQSGLLRILNV
                     SHPDEPYIESTMEIGTNMISVAVVNENWAVITSGSYAFPIYDIILSLVNIEDVSNPFV
                     TDALTIGSHTTVYTNNNICVNNGVIYVVTLNDLLLFKANDGTLTNIGSTTLYGKHGCG
                     IYTNNSDYVYVAGGQYGFQMYNVTNPAAPFMINFNFVSGTCWDVYVEDGVAYVAAYDG
                     GFATFDVSQNMVQPLGLLTNTLGNAKAVKVYNKIAYVGLENGSIQLFDVEDPSQPMSL
                     GWYLTPGTRVNDITLDSIGGINYLYVANELDLLIMEIKNQVGISEEPALIHNVNVFPN
                     PATDKATIEINLPTPQNVKINLYNIIGNKVGRILNNKIASGRFQFEVDTKELPSGIYM
                     VEVLSGDSRCVEKLIIKDK"
     gene            8439..10424
                     /locus_tag="PLB65_06440"
     CDS             8439..10424
                     /locus_tag="PLB65_06440"
                     /inference="COORDINATES: protein motif:HMM:NF019959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HOQ78203.1"
                     /translation="MKKFLLIFSMIFVHLLVISQTFNDVTTASGIQVLPGMGDLALWI
                     DYNNDGWLDFFGGTENETFLYKNNGDGTFTNVSASSGLTGLMPRTAAVGDFDNDGYED
                     LLICSFHMSVPVTIYRNKNGEGFEEAYSTNNSAHRAIWIDYNTDGLLDFIVSGESGNS
                     YLYLNQGNNNFVNASEQISFTSGTSSVPAAGDFNNDGLPDIYFASANTTKTNRLFMNV
                     AGQSIKDATYTAGVSDFRRSVSVAWGDMDNDGFLEIYYGNISSNRNVLLHNKGNCKFE
                     DVTLAAGVQDAGDARTCTWQDINNDGWCDLFTTNHVNPNKLYINNADGTFTNIANSVN
                     ISGPQDGFGVSWGDYDKDGDLDVLIAGHSYGMVLMNNNMNIEFSFLNIKLVGVYDNRS
                     AIGARVTLYCDGQMQIREVNGGRGATGQDALELHFGLGEATEVDSIHIKWPSGALQKV
                     FNINANQFITIEQEGNIPPTRFRLHYPLPDSVYTAPEVEFAWQKSTDPDSPDLPLYLL
                     HIFTSQNDTIIGPLADTIHSVQMANWMSSDTCYWHVQASDGIDITQSWDQFQLQYESG
                     TYISQQVVYQMNGFSVKHYYYAKENGKLHIQLESDNEKSIIGEIIDLNGKVLNQFQIT
                     IPKGISQHNISIPNSRNICLIRLQSGIQQLSFKLTSQ"
BASE COUNT         3403 a         1821 c         1862 g         3641 t
ORIGIN      
        1 aatagctctt gagtgtgtaa aagtatctat tttgcaaaat taaaatagaa tatccagtaa
       61 gctcgttttt ctaaaaaagg attttatttt aatcaatata gttgataaca ataacggtac
      121 tgattatatt actaattcag ataaaaatat agtaacggtt ttaatttatc aaaaaaaaga
      181 tgtttaccag caaacgttct atgatttggt gatataaccg tgtttttcag catacttact
      241 aagacctatc gctgttttcg cttttgtttt actaaaaata ttctttcgat gggtttcaac
      301 agtcctttca ctgatataca atttttctgc tatttgtcga gttgaaaatt cttttgaaat
      361 taatatcaga atttctattt cacgttttgt aagcgttgca acgtgcttgt tatcatttat
      421 ccaaggtttt gtgtttttgc ttgcgctact tttctctact ttgttaaata ttatctcctg
      481 tatttctgta ccaaaatatt tcaaaccttc tgcaacaatt tttatcgctc gtattaattc
      541 ctgaaggctt tcggatttga ggatataacc agaggctcct gcaacaatca tttgttctac
      601 atattcatat tcatcaaaca tggtaagagc taatatttta gcctctggat tgattttttt
      661 aagccgtatg gtaagttcaa ctcctgatat ttgcggcata ttgatatcca ttagatagac
      721 attaacttta ggttgctcat ccagattaat aaattccaat ggattaagtg aatatccggc
      781 tattttaaag ccctccacct cagcaagtaa tgaacacaaa ccctctacaa aaagtttgtg
      841 atcatcaatt atataaatag aaatttcact catattacta ttacattaat ttgttttgga
      901 aggtataata aaagtaacta tagttccttt gcctatttta ctgtcaacaa acatattgcc
      961 tttaaagcct tcaattcttt tttgtgtact cttcattcca agcccattat ttttctgtaa
     1021 atccgaaaca tcgaatcctt gtccattgtc ttcgatcata actgtaatat catcttggtt
     1081 accaacaatt tgtatgttaa tttctgttcc tttggcatga ctcaaaacat tgttaatact
     1141 ctcaagtatt gaacggtaga gtacatgttc tacgtatggt tcaagtttgt cgtttattgc
     1201 atacatatca aggtttactt gatagttgtt agtttcattt aatcttacta ctaagttctt
     1261 tatagcagct tcaaatcctt tttctatcag aatgattgga gccatattct gcgaaatctg
     1321 ttttatctct tttagtattt catttatagt gtcaagggta ctattcacca tttttgctcg
     1381 tctatcgttg atactatcca attgacctgc aagtgctgta atattaagtt ttaccagtga
     1441 aagtagcgga ccaacacgat catgaagctc aagacctaat aattttcttt cctgtatttc
     1501 agcatcaagt gctgcctttg ctctctcttc agcaactttc agatttgcat cgttaatctt
     1561 tttttgttgt tctaccttta ctttattggc atagagaaaa tataaagata caatcaatac
     1621 aataatgatt aagaaaatca atacgattat ataaatcatt gtatttcttt gctttattaa
     1681 atattgttgc ctttctattt cgagtttttg tatctcctta tccttgctta aatgttgtat
     1741 ttccctgtta taaatcccat gcagcttgga tttgtccata attttattgt tcacgtcagt
     1801 tccttcttta aaatgactca aagctatatg gtaattacct tttgtctcca tgattttact
     1861 aagcagcatg tggcaatcaa caatcagttt ttcatcatct aaatcattgg ccatattaag
     1921 agcttcaagt gcatggtttt ccgattgttg gatatctcct tttttaagat aagcttttgc
     1981 aaacccgata tgaatgatac tcaacgtgtt tttattttga attcgcttaa cttcagtttc
     2041 actaagttta taatagttaa gggcttgatc caaatcgtta agcgctatgt aaatatcgcc
     2101 ctttgttttt aaagctaatg agagactata aatttcctga atactatcag caagtctaat
     2161 agcttgatca attacaacta atgcagaatc atattttcct cctagctgat aattttggct
     2221 aatgtgtgtt aagactaatg tttgacctgg aaagattttt agtttttcaa aacctgaaaa
     2281 actctctttg taatgacagt tagccttctc tgtgttgcca agtgcatcgt acaaaacgcc
     2341 aaggttacaa tttaccccgg ctactttatg ataagcgccg tcaagttcat aataagtaag
     2401 agcttcaaga taattagtta gggcttcagt gtaatttctt gcaaaaaaat gaacaccacc
     2461 tatattatta tatgaatcac ctaatcgttc attatcatcc gaatccattg caagtttgag
     2521 tgctttgtgg taacactcta aagcttttat gtactctgat tttattgaat aacaggtgcc
     2581 tacaagattt agtagtctaa cctgccagtc aatatgatta atttgctcca ctattagcaa
     2641 ggcttcctta gccaatagtt gggcgctatc aggagaatga tatacaaggt tttgaagctt
     2701 gaggaaaaga gtgtctatgg gctggccgtt gattttgata cactctctgt tttttttatt
     2761 atttcctgat aaaagccaaa tctccgactt tatttgttgg gaaagcataa aaattaaaat
     2821 taacaagcca aatgacaaga aacttttttt cattattaaa aaagatcaaa gattgattct
     2881 tacaataaaa ttaaattttt aaaatgcatc aagcaaaatt aaaacttaat gcaataagga
     2941 aaataatttt atattccaaa gccttaaaaa taccttgtac tactgagaat ttttacctac
     3001 ttttactgaa tagacgaacg aaaaattgtg tataattttg catttgaatt tttttcaatc
     3061 gttttattta ataaattaat ttcaaaaata atttatttat gaaaaagctt ttacttttaa
     3121 catttttaat ccttacccta aattgggtag gacttgctca ggtaggaagt agttataatt
     3181 tcacacagac tcaactcacc tattatccgt tgactgaaga tatagttctt tggtctggtt
     3241 cttttcaata tgagaccccg atagagattg aaataccaga ttttcctttt gacggcacaa
     3301 tttacaacag tgtttttgtt agctcaaatg gattcataac atttggttct gctcctacgg
     3361 taaataacac taagccaata agcagcgatg aggattatag cggagccgtg tcagcattta
     3421 gcggcaattt gactccagcc ataacaggta acccgcagat aagttacaaa ttggatgatg
     3481 atttttttat tgttcaatgg caggacgtga gaagatattt agtttatgag gatatcatca
     3541 gcttccagat aatacttgat tttaacgaag gaataatttc cgttgtttat ggcggagata
     3601 ttgcctcagg gcaaaatagc gcttttcctc aggttgggct tagaggccaa acaaacaact
     3661 tcccttctca tattaataac cgtatgattt taataaatgg tgggaattgg attaattcac
     3721 agccaggtat tgcaaacaac agcaccatgt attttagaaa cacctcccct gaaactattc
     3781 catcacccgg actcactttt acatggactc ctcaaagtag tgctccatta ttaatttcac
     3841 ctgtttcaca tgattttggc gaggttttag tctcaatgac atcagatcca ttttcatttt
     3901 ctttatccaa cacaggaatt atgtcggtaa caattgactc tgcatttata acaggtgaaa
     3961 atgctgacga attccaaata gttgacctac caacttttcc tattgtactc gacgaatttg
     4021 aggttgtaaa tataaaagta accttttctc caactgaagt tggtttgcga aatggtaaat
     4081 tgatagttaa taccgatggt ggggattttg aggccgaact taccggtaca ggatatgaag
     4141 agggaatttt tgatttacca tttattgagg gttgggatag tgggaatttt acagaaaaca
     4201 attggtcttt tataaatcca acgtcaactt ggaggatatc acctggaaca atggggaatc
     4261 cgcctccttg tgcccagttt cactacagcc cacgtcatta caattattcc acatcattaa
     4321 ttagccctac gttaaatatt tcaaatacaa taaattggga attatcattt gatatgaagt
     4381 tgtcaaatta tttaaactcc ggcacagaat attttgatgt ccatatgctg ttagatagta
     4441 actgggtatt aattgatagc tttgtaaact ttttcgatct tgaatggact acctatacct
     4501 ataatctcga tactcttcag ggtgatgtta ctcgtattcg ttttgaaatg catggggaag
     4561 ataatgataa cctgaataat tggtcaatag ataatattgt ttttaatgga gaaattgtat
     4621 atctttttga acttgatcct gaacatgttg attttggtga agtgatgaca ggcaccactt
     4681 ctgatccttt tattgttaca tatgcaaata atggattaaa cgagattgga gtcaacagtt
     4741 cagttataat cggcgatcaa tctgattatt ttattctaac agataataat caatatccat
     4801 ttattttatc ccctggacag agcgccgatg tttctgtaca gtttgcgcca ttggaagagg
     4861 gtaatgcatc agcatcgtta gtaatatcta ctgaagtagg aacttttaca tctacgctcg
     4921 gcggttttgg ctatgatgaa tatatcggca caattccctt ttttgaagat tgggctacag
     4981 gctcatttaa aacaaggcaa tggtcatttg atcaaaccga aacaaattgg cttattcaaa
     5041 ataatagtgg taatccagcg ccaacagcca gatttagttt ttcaccaatt attgagggct
     5101 atagccaatc ccttatcagt ccgcgcattg acgttccagt ttcaaatcag aagattgctt
     5161 tgtcctatga tcttaaactt gctgattacg aaaccagtgg caccgaatgg ataaaagtat
     5221 atatatggac gggatcagaa tggcaagagg ttgctgcaca ttcaaataac ggcaacatgg
     5281 catggcaaaa tttccagtat tatattaccg attatgtaca aaatgaaacg agaattagat
     5341 ttgaagcttc gggcgtcaac tcggcaaata tctcgaattg ggaaattgat aatatctctc
     5401 ttgacttttt ggatttacct gttattaatg tagaaccaat ggcattaaca caggaaattc
     5461 catattcggg cactgctgtt caggaggtaa taataagtaa tgaaggtact gccgttttaa
     5521 attatagtat aaccataaat tatatcgcgc cggctacagg aaattggcta agtgtattac
     5581 ccacctcggg cgaagtagct gtaggcaact cacaaatagt tcaggtatca tttgatacaa
     5641 atggacttac cataggaaat gtttacacag ctgaactgat tgttgaaaac aatgaccctg
     5701 ataatcctga agttaccatt tcggttacat taaccgtttt aacatcgcaa aatgaattgg
     5761 tcgcaaaaaa gataattatt tacccgtctc ctgctaagca aattataaat ctacaaggca
     5821 ttaatgagat catcctcatc caaatattta atagttcagg gcagttaatc tttggagttg
     5881 aaaatgaggg tgaaaatatc aggcaaattg atgtttccaa tttaatatca ggctcataca
     5941 caatgcgctt tatcactact caaaatacct atataaatca caaattgcaa atcattaagt
     6001 aacaacataa acatttgaat cttatgaaac gagttttaat ttttagcttt ttaagtctgt
     6061 ttgttttctt ttcggtaagc cgtacagaag cgcagtcatg ggggaacaat aatgattaca
     6121 tagttcctat ttctcatagt ttggcaaatg gcataatgag tcaatatccg ccaacaacca
     6181 gtatagtgga aaacaatatt ctctattata ctgatcttta tggttttcat atttataatg
     6241 taagcaatcc tcagagtccg gttgaaattt catccttacc tattccgggg cgagctcgac
     6301 attttacaat aaaaggaaat tatgcctata tattaaatga attaggagtt gcatttgtaa
     6361 acattgaaaa tatttattta cctgaaattg aagacattgc atggattggc ttcaaccctg
     6421 tacgggttat tgttgacgaa gaatgtttgt ttttagcagc agacgatggc gtatatgcat
     6481 attctgtttc cgacacccat cagctatcat tattaagtta tacttatgtt gctcctgctg
     6541 ccactacatt tgccggattt attaagcatg ataagtatat ctattatgct aatcaagtac
     6601 ggttacatgt ttttgatttt gaagaccccc aaaatgttat cgaaacatcc gttagtaatt
     6661 ttacgggagg tggtagttgt tggggaggat tagcaattaa agatgattat ttatatgtcg
     6721 taacgacttt aaagttacat attttcaata tttccaatcc atcaatacct caaatagtat
     6781 atacaggatt gcctacaagc cacactatat attcaatagt tgttgagaat aacaatttag
     6841 tgttgaatca tagtaacaat gggaaattta ctattcttga tatttcaaac cctactaatc
     6901 caaatgttgt ctatgcttat ccgggaggtt ggtttcatca ccttatctat ctgggagctt
     6961 taaaagataa tattctttat gttttagata acgaacagga ggtatataat ggatttagcc
     7021 ttcacctgat agatattaat aatgtatcat cacctacgca aattactgag ataaaatcta
     7081 cacccggaag aaccagatca gtagcgttac ttaataaaaa taataaatcc cttgctttag
     7141 tggcacagga taacgaaagc agaagcggac aatccggact tttgcggata ctcaacgttt
     7201 cacatcctga cgaaccctat attgaaagca caatggagat aggtacaaat atgatttctg
     7261 ttgctgtagt caatgaaaac tgggctgtta tcacttcagg tagttatgcg tttccaatat
     7321 atgatataat cctatcactg gtaaatattg aagatgtatc gaacccattt gttactgatg
     7381 ctttaacaat aggaagtcat acaacagtat atactaacaa caatatttgt gtaaataatg
     7441 gggttattta tgttgtaacg ctaaacgact tgctactttt caaagcgaac gacggtacct
     7501 taacaaatat tggtagtaca acactgtatg gtaagcatgg gtgtggtatt tatacaaaca
     7561 attctgatta tgtatatgtt gcgggcggcc agtatggttt tcaaatgtat aatgtaacta
     7621 acccagcagc tccatttatg attaatttta attttgtctc tggaacttgc tgggatgttt
     7681 acgttgagga cggtgttgct tatgttgctg catatgatgg cggtttcgcc acttttgatg
     7741 taagccagaa catggtacag ccacttggtc tactaactaa tacacttgga aatgctaagg
     7801 ctgttaaagt ttataacaaa atcgcttatg tcggcttgga aaatggcagt attcagcttt
     7861 ttgatgttga agatccgtct cagcctatga gccttggatg gtatcttaca ccaggaacac
     7921 gggttaatga tattacctta gattctatag gtggaataaa ttatttatat gttgcaaatg
     7981 aacttgattt acttattatg gaaattaaaa atcaggtagg aatatctgaa gaacctgctt
     8041 taatacataa tgtaaatgta ttccctaatc ctgcaaccga taaagcaact atcgaaatta
     8101 atttgcctac cccacaaaac gtaaaaatta atctatataa tattatcggt aataaggttg
     8161 gacgtatttt aaataacaag atagcatcag gaagatttca gttcgaggtt gacacaaagg
     8221 aactcccgtc aggaatttat atggtagaag tattatctgg agattctcgt tgtgttgaaa
     8281 aattaataat aaaagacaaa taaaccttac ttaatatgct aatccttatg tagtgctaat
     8341 taactatgga caccggaaaa tggttgctat aattttttat agcaccattt tccgatacta
     8401 actaaatcaa tttctaagaa ttatataata ctatcaatat gaaaaagttt cttttaattt
     8461 tctcaatgat ttttgttcat ttgttagtaa taagtcagac ttttaatgat gtaacgactg
     8521 cctctggtat ccaggtatta ccagggatgg gcgatttagc gctttggatt gattacaata
     8581 atgacgggtg gcttgatttt tttggaggaa cagaaaatga aactttcctt tataaaaata
     8641 acggggatgg aacatttacc aatgtttcgg caagttcagg cctaacagga ctcatgccaa
     8701 gaacagcagc agtaggtgat tttgataatg atggctatga agatttgtta atttgttcat
     8761 ttcatatgag tgtaccggtt acaatttatc gaaataaaaa tggagagggt ttcgaagaag
     8821 cttattcaac taacaattca gcacacaggg caatttggat agattataat acagatggtc
     8881 ttctcgattt tattgttagc ggcgaatctg gaaattcata tctttatctt aatcagggta
     8941 ataataattt tgttaacgca tccgaacaaa ttagcttcac cagtggaact tcctcagttc
     9001 ctgctgcagg agattttaat aatgacggat tgcctgatat ttatttcgca tcagcaaaca
     9061 ccactaaaac taacagatta tttatgaatg tagcaggaca atccattaaa gatgccactt
     9121 atactgctgg agtttccgat tttcgtcgaa gcgttagtgt tgcatgggga gacatggata
     9181 atgatggttt tctggaaatt tactatggta atatttcatc taaccgtaat gttttattac
     9241 ataacaaagg aaactgtaag tttgaagatg ttactttagc agcaggcgtt caggatgctg
     9301 gcgatgcaag aacttgtaca tggcaagaca ttaataatga tggctggtgc gatttgttca
     9361 ctacaaatca tgttaatccc aacaagcttt acatcaacaa tgctgatggt acgtttacaa
     9421 atattgctaa ttcggttaat attagcggtc cacaagacgg ctttggagta agctggggag
     9481 actacgacaa agatggcgac cttgatgttt taatagccgg acactcgtat ggaatggtac
     9541 tcatgaataa taatatgaat atcgaatttt cttttttaaa tataaaactc gtaggagttt
     9601 atgataatag gagcgctatt ggagccagag taactttata ttgcgatggt cagatgcaaa
     9661 ttcgggaagt taatggtggt aggggggcta ccggtcagga tgcattagag ctacattttg
     9721 gattgggtga agcaacagaa gttgacagca tccatattaa atggccaagt ggagcattgc
     9781 aaaaggtatt caacataaat gcaaaccaat ttatcactat tgaacaagaa ggaaatatac
     9841 ctcctacaag atttaggctt cattatcctt tacccgattc cgtttatact gcaccagaag
     9901 ttgagtttgc atggcaaaaa tctacagacc cagattcacc agatttacct ctatacctac
     9961 tacatatttt tacatctcaa aatgatacta ttattgggcc tttagcggac acaatacatt
    10021 cagtacaaat ggcaaattgg atgagcagcg acacctgtta ttggcatgtt caagcctctg
    10081 atggaattga tattacacaa agctgggatc aatttcaatt gcaatatgag tcaggaacat
    10141 acatttctca acaggtagtg tatcagatga atggattttc ggtaaaacat tattattatg
    10201 caaaagagaa tggtaaatta catatacaac tggagtctga caacgaaaaa agcatcatcg
    10261 gcgaaattat tgatttaaat gggaaagtcc ttaaccaatt tcaaatcact attcccaaag
    10321 gcataagcca acataatatt tccattccta attctcgaaa catctgtcta atacgcctac
    10381 aatcaggtat tcaacagcta agttttaaat taacctcaca atgaaagtta ttaatatctc
    10441 aaagaacaga tgagtgagtt ttcataggta acttttcttt tggttaaaaa cacccataaa
    10501 cttttcgttt tgggtgtttt ttcatccagt cttttacttt agattaggtt tttggatata
    10561 gacaacagaa gatgtgtaaa gtacaatttt tctttaaacc tcggatatta caaagtccaa
    10621 aagtcaaatt tcattactgc ttattgctat tatcttttac tattaacagt aaaaccccaa
    10681 ttattgtgaa ggttccgcca acaatctgat agcctgcagg gagtttt
//
LOCUS       DAOSTH010000055        10710 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_6029_length_10710_cov_17.269263, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000055 DAOSTH010000000
VERSION     DAOSTH010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10710
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6029_length_10710_cov_17.269263"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            626..1375
                     /gene="lptB"
                     /locus_tag="PLB65_06445"
     CDS             626..1375
                     /gene="lptB"
                     /locus_tag="PLB65_06445"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HOQ78204.1"
                     /translation="MDKHKIRAQNLVKIYGKRAVVNDLSLEMSQGEVVGILGPNGAGK
                     TTTFYMIIGLAKPNNGKVYYDDKDISHKPMYKRARLGLGYLAQAPSIFAKLTVEENVL
                     AILQTLKLSRKEQKNRLEEALAELNLTLVAKQKAYTLSGGERRKLEITRALVTNPTFL
                     FMDEPFAGIDPIAVADIQDIIEKLRNKDIGIMITDHNVLETLKIVNRAYIIYEGKIIV
                     SGTSLELINDEKAKELYLGDRFLQSPFEQRL"
     gene            1372..2829
                     /gene="rpoN"
                     /locus_tag="PLB65_06450"
     CDS             1372..2829
                     /gene="rpoN"
                     /locus_tag="PLB65_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOQ78205.1"
                     /translation="MTAIDQYLSLKQKQELALKPKMLQSLKMLALPILELESYIKQEL
                     INNPLLELREEKEEEDLDESKFEQSIPESPTPIEDSEISEEHIDTLAEARELTEILDN
                     WNEYHSGSENWSADIEHNHTESLIRYEENGKVKFLEQLYPYDLPEDEVDFITEIVDSC
                     DSYGFLPKGYNLINLAKKYHINAERAEELHNLVLSFSPKGISARNITECLLAQLTDEQ
                     KENRIIMGLISDQFENLIHRRYQKIASYFGVSEDTIFAAKEQISKLDPKPGLRITTTN
                     AAYVYPDVTMKLIDGEYEIIINDNITPNIIISPQYRRMISRGYFDKESMQYVRERINS
                     AKFLIKSIYMRMRTLERVSRSIIKHQHDFFYLGTGQMHPLTYAVIARDLGVNESTISR
                     VVKYKYAETPYGIYAFKDFFTSTAGYDDDYQSISRQKVKTHLIRLIETENKKKPYSDQ
                     ELVDLLKEEGLNISRRIITKYRDELGILNSHLRRK"
     gene            2830..4239
                     /gene="lpdA"
                     /locus_tag="PLB65_06455"
     CDS             2830..4239
                     /gene="lpdA"
                     /locus_tag="PLB65_06455"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOQ78206.1"
                     /translation="MLYSQVIIIGGGPGGYEAAIRLSQYEIPCLVIEKERLGGTCLNW
                     GCIPTKALVKSAELYSEMLSADNFGLPKQNQEIEYQNVWSRKNAVVEQLASGIEFLFK
                     KRNIPVVKATAISVAKQKENYIVTTLEGESYSCEYLILATGSKVKGLPGIEIDEKDIL
                     SSTGILKMQELPEELVVIGGGVIGCEFASVFSTFGVKVTIIEFLPRLIYQEEEEISKR
                     LAMQFKKSGVKVLTNKGVQNINKKGSRLELKLSDDTILETEKVLLSVGRKPVNNLEWK
                     QIEELKTEKGAVIVDDFMQTSLDKIYAIGDLTGKLQLAHTASKQGLFAVEHIASRIYP
                     EKYKVPAKPINYINIPRCTFTHPEIASVGYTQKEAEEIFKEVLTGKFPFSANGKAVAM
                     GNPNGFVKIIARKDTSEIIGMHIMGPNATELIAQGAIMIANQNKTADVESIIFAHPTL
                     SEAIMEAIEDLQNLSIHKI"
     gene            4259..4795
                     /gene="raiA"
                     /locus_tag="PLB65_06460"
     CDS             4259..4795
                     /gene="raiA"
                     /locus_tag="PLB65_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOQ78207.1"
                     /translation="MQITITARHFELTKAIRDYVESACSKLNKYFDQIIAMHVVLALE
                     NSRNICELSLHASKFKLQSQAEEMDMYLAIDNAVEKMEAQIKKLKDRITDHQKRALKQ
                     QFETYSHATDFELEPKSHMRKTIKTKRVVTESLSLSEAVEKLEASKEDFLIFKNIESD
                     RINVLVTKDHNVYKLLEP"
     gene            5000..5989
                     /gene="hprK"
                     /locus_tag="PLB65_06465"
     CDS             5000..5989
                     /gene="hprK"
                     /locus_tag="PLB65_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOQ78208.1"
                     /translation="MKEITVYDLFDTKKKDLALSLITEPETLNKKIGSPYLNRPGLAL
                     AGYLELFAADRIQVLGETEVRYLQSLTEEDLITRIRNMFKMDIPCIVISKGLTLPPVI
                     EYIANELNIALFSSRLSTINLIENLSRYLRDIFALEKTIHATLVDVFGLGILLTGKSG
                     IGKSECALDLVHRGHSLVGDDLITLRYIEDKLIGKPGREFGYFMEIRGVGFINAERVF
                     GIERTRKQKTIDLQIELMPWQENMDYERIGLVSNYAEHLGVKIPIIYLPVSPGKSVSV
                     IVEVAAMNIILKGVGYDAAEDFNRKVNEEIRKKTMLKNIEGKSDETDRGDRNQ"
     gene            5958..6221
                     /locus_tag="PLB65_06470"
     CDS             5958..6221
                     /locus_tag="PLB65_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOQ78209.1"
                     /translation="MKQIEVTVTNKLGLHARPSALLVKAATKYRSDFFIEKEGMRING
                     KSIMGVMMLAAECGSKIKLIADGVDEDYLLEEIKTMIESGFGE"
     gene            6222..7955
                     /gene="ptsP"
                     /locus_tag="PLB65_06475"
     CDS             6222..7955
                     /gene="ptsP"
                     /locus_tag="PLB65_06475"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOQ78210.1"
                     /translation="MQKIYGTSLNTGIFIGNALHIALPEPAIETGNIRQEEIPKEISR
                     LQSSLSIVEEDINLELKTSNLQAQDAEIISAQREILRDPEIYNQIETAIRNKLQYATQ
                     AVSECFANIIKHFETMKIETFAVRAADYRDVAMRLLNALSGVRNTLKIKIEPNQIPVF
                     IEIVPSLVSKLAQEGIKAYLVAKGSYNSHSSILCRALGLVAIANIPDLNVAVKNNDLL
                     IVDGYTGLLIVNPDDQTLEYYRKKAEAERHRQEKLKQLKITDSITANGINIELFTNIG
                     VPEELEVIKNLSCAGIGLFRTEFLYLGRQNLPDEEEQFAVYKELAEKMKPLPVTIRTF
                     DLGGDKLSPLGYETPEENPYLGNRGIRFSLSQPDIFYTQVRAILRASAFGKVQIMFPM
                     IIDTQDFIEAKKFVQNCQRELSQHSISFDPEIALGAMIEIPSAALCSEELAKECDFFS
                     IGTNDLVQYTLAADRNNEMVAKYYIQHHPAVLCLIKKTIISAEKYHIPLSVCGEMASN
                     YKYIPLLIGLGIRELSVNPGSFFSAKAIVQKCDAKLFKATEEINCSTPLSEVENLINN
                     ILAPYYQDKEE"
     gene            7956..9308
                     /locus_tag="PLB65_06480"
     CDS             7956..9308
                     /locus_tag="PLB65_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOQ78211.1"
                     /translation="MLKYEYHNLLASKQMLSILPKDKIFDSEHQVKNIHEEIMAERRA
                     KLLGFYDLPENSTRHIDDFIANLDPVFDTMLVLGIGGSALGNKALYSALKTEAELPRK
                     LVVYDNVDPVFLYEILSQINLETTLVNVITKSGTTAETMAGYMIVADILKRKFPKDYQ
                     KRIIVTTDKERGFLRQVIKKEGYKSFTVPDNVGGRFSVLSDVGLVSSAFTGINIQELL
                     SGAKAMRERCSEPDIMENPAYLNGLIHFLYYRLGKNISVMMPYSNSLYDLADWYRQLW
                     AESLGKRKDIKGREVYVGQTPVKALGTTDQHSQVQLYTEGPNDKVITFITVENFKHDY
                     IIPNLHPDHEEVNYLGNRKLSDLLNAERLATEIALSKAMRPNANIVFPQIDEYHLGEF
                     IMLYEIQTILTGKLLRINPLDQPGVESGKIATYALMNKKGYEKEHAEIEKYKQDRDIT
                     "
     gene            9344..9508
                     /locus_tag="PLB65_06485"
     CDS             9344..9508
                     /locus_tag="PLB65_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78212.1"
                     /translation="MKNRILNKRVKSYPVMEADMIFKSKNYDYENNIDRNWQNENNAI
                     VVRHNVELKN"
     gene            9685..10554
                     /locus_tag="PLB65_06490"
     CDS             9685..10554
                     /locus_tag="PLB65_06490"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOQ78213.1"
                     /translation="MKAILTIAFVLFCICLSAVDYIDVVYTKNGDVLKGIIIENIPND
                     KIKVDINGVVVTVMYDQIIKLAKEEIDTTSTKVTKRNAISKTVNENDSDLPFHKKYGL
                     LGGVNSATAKVDNSEGVDISSVWGFSFGGFATFQTSPNIIIQPELIYTGKGFKTEINL
                     DDYDYEYKETDEWKFAYLEVPVLFKYVFQTGNMYITPFIGPSLSIAMSGKVSYDSYQY
                     YQNYEEEDSGSMDIKNQMEDIDFGLNFGTDLYFSEKIFLQLKYDLGLSKIFTKSVLES
                     KNRVLSLSMGVSF"
BASE COUNT         3700 a         1772 c         2104 g         3134 t
ORIGIN      
        1 cccgacctag tggttaacag ccacccgctc taccgctgag ctattccgga acacaattca
       61 ttataccttt tcttggagag cccttttttt gtcaaccaga atttttagtt tgtattttct
      121 gaatggtttt aagtatatcc ctattggtag agtattggat tatttaccat aggtgaagca
      181 agattttact acttttcgga tactgacatt ttgcatattt acttcagaaa ttgttctgct
      241 aaagaatccc ggtttaaact gcctcatttt aaaccttcct tagttatttg tggtagagct
      301 gccgtggtgt tcttcggcaa aaacccacag aggtacggtg gcatctgcac ggtaggtcat
      361 taagcaagaa tgggataaaa ctaaataaat tccacaggct tgctgtggca aaattgtgcc
      421 cagcaaaaac agataggtat attaatccac ttattcttcc ttatccgacg atactataaa
      481 ataaacaggt ggcaagatat aaagggtaag tttttggcat taagtaaatt cgctactgat
      541 tacagttaga aatgagctta gcaaaaatca atccatatgc aagtatttct tgacaaaaaa
      601 atggctttcg tttaagtata aaaatatgga taagcacaaa attcgagctc aaaacctcgt
      661 taaaatatac ggtaaaagag ccgtagtaaa cgacctatct ttggagatga gtcagggtga
      721 ggttgtaggt attttaggcc ctaatggggc tggtaaaacc actacttttt atatgatcat
      781 cggtttagca aagcccaata atggtaaagt ttattatgat gataaggata tctctcataa
      841 gccaatgtat aaaagagcgc gtttgggctt gggatatctg gctcaggcac cttctatttt
      901 tgctaaattg acggttgaag aaaatgtttt ggcgatttta cagaccctta agctaagccg
      961 taaagaacag aaaaatcgtt tggaagaagc tttagctgaa ttaaatttga ctttagtggc
     1021 aaaacaaaaa gcatatactc tttccggagg ggagcgaaga aaactggaaa ttactcgtgc
     1081 tttagtaacc aatcctactt ttctttttat ggatgaacct tttgcaggaa ttgatcctat
     1141 cgccgtagct gatattcaag atataattga aaaactcagg aacaaagata ttggaataat
     1201 gattaccgac cataatgtac ttgaaacttt gaaaattgta aatcgcgctt atattatcta
     1261 tgaaggtaag attattgttt cagggacatc cttggaactg ataaacgacg aaaaagcaaa
     1321 ggagttatat ctgggcgacc gatttctgca aagccctttt gagcaacgac tatgactgct
     1381 attgatcaat acttgtctct aaagcaaaaa caagaattgg ccttaaagcc aaaaatgctg
     1441 caatcattaa aaatgcttgc tttgccaatt ctggaactgg aaagctatat aaaacaagaa
     1501 ttgatcaata atcctttatt ggaactacgc gaagaaaaag aggaagaaga ccttgatgaa
     1561 agtaaatttg aacagtcgat tccagaaagc cctactccta ttgaagatag tgaaataagc
     1621 gaagaacata ttgatacttt agcggaagca agagaactta cagaaatttt ggataactgg
     1681 aatgaatatc attccggaag cgaaaactgg tctgccgata ttgaacataa tcacacagaa
     1741 tctcttattc gctatgaaga aaacgggaaa gtaaaatttc tggaacagtt atatccctat
     1801 gatctacctg aggatgaagt agattttatt actgagatag tagattcctg tgatagttac
     1861 ggtttcttac ccaaagggta taacctaatt aatctagcta aaaaatatca tataaatgca
     1921 gaacgcgccg aagaactaca taatcttgtt ctttctttta gtcccaaagg tatatcagct
     1981 cgcaatataa ctgaatgttt actagctcag cttactgatg aacaaaaaga aaacagaatt
     2041 attatgggtt tgatatctga tcagtttgaa aatctgattc atcgtcgata tcaaaaaatt
     2101 gcttcttact tcggggtttc tgaagataca atatttgcgg ctaaggaaca aatttccaag
     2161 cttgatccta aacccggttt acggattaca actaccaatg cagcttatgt ttatcctgat
     2221 gtaactatga aactgattga tggtgaatat gaaattatca tcaacgataa tattacgcca
     2281 aatataatta tcagccccca atatcgaaga atgattagcc gcggttattt cgataaagaa
     2341 tcaatgcaat atgttagaga acgcataaat tccgctaagt tcttaattaa atccatctat
     2401 atgcgaatgc gaactttgga aagagtttcc cgttctataa taaaacatca acatgatttc
     2461 ttctatttag gcacaggaca aatgcatcct ttaacctatg ctgttattgc tagagacctg
     2521 ggagtaaatg aatctactat tagcagagta gtaaaatata aatatgcaga aacgccttac
     2581 ggaatttatg cttttaaaga tttctttact tctactgcag gttatgatga tgattatcaa
     2641 agtatttcca ggcaaaaagt gaaaacccat ttaattcgtt taattgaaac agaaaataag
     2701 aaaaaaccct atagtgacca agaactggtg gatctcctta aagaagaagg acttaatatc
     2761 tccagaagaa ttataacaaa ataccgggat gaattgggca ttttgaacag tcatcttagg
     2821 agaaaataaa tgctatacag tcaagtgatt atcattgggg gaggccccgg tggttatgaa
     2881 gctgccatcc gtctttccca atacgaaatt ccctgtttgg ttatcgaaaa agaacgctta
     2941 ggaggaacct gcttaaattg gggctgtatt cctactaagg cattggtgaa aagtgcagaa
     3001 ttatattctg aaatgctttc tgcagataat tttggcttgc ctaaacagaa tcaggaaatt
     3061 gaataccaaa atgtttggag ccgcaaaaac gctgttgtgg aacaattagc cagtggcata
     3121 gaatttttgt tcaaaaagcg taatatccct gttgtgaaag caactgctat ttccgtagct
     3181 aagcagaaag aaaattatat tgtaacaacc ttagaaggag aaagttacag ctgcgaatat
     3241 ttaattttgg ccaccggtag taaagttaaa ggattacctg gaatcgaaat tgacgaaaag
     3301 gatatcctgt cttcaaccgg gatcttaaaa atgcaggaat tacctgaaga acttgtagta
     3361 attggtgggg gagtaattgg ttgtgaattt gcctccgttt tttccacttt tggggtgaaa
     3421 gttactatta tagaatttct ccctcggtta atatatcagg aggaagaaga aatttccaaa
     3481 cgcctggcta tgcaatttaa aaaaagtgga gtgaaggttt taaccaataa gggagtgcaa
     3541 aacataaaca aaaaaggaag tcgcctggaa ctgaaactaa gtgatgatac tattttggaa
     3601 acggaaaaag tgctgctgag cgttggtcga aaaccggtaa ataatctgga atggaaacag
     3661 atagaagaac tgaaaacaga aaaaggggca gtgatagttg atgattttat gcagacgagt
     3721 ttggataaaa tctatgctat tggtgatctt acaggaaagt tacagcttgc acatacagcc
     3781 agtaaacagg gtttatttgc agttgaacat attgcttcaa ggatatatcc tgaaaaatat
     3841 aaagttcctg ctaaaccaat taactatata aatatccctc ggtgcacttt tacgcatccg
     3901 gaaattgctt ccgttggcta tactcaaaag gaagctgaag aaattttcaa agaagtttta
     3961 acaggtaaat ttcctttttc agcaaatggt aaagctgtgg caatgggcaa tcccaatgga
     4021 tttgttaaaa tcattgcccg aaaagataca tccgaaatta tcggaatgca cattatgggt
     4081 cctaatgcaa cagaattaat tgcacaggga gccataatga tagccaatca aaataaaacg
     4141 gcagatgtcg aatccattat ttttgcacat ccaactcttt cggaagcaat aatggaagcg
     4201 atagaagacc tgcaaaatct atcaattcac aaaatctaat aagaaggagg aatcccaaat
     4261 gcagatcaca attacagccc gtcattttga gctcaccaaa gctatcaggg attatgtgga
     4321 atcggcttgt tcaaaattga ataagtactt cgatcaaatt attgcaatgc atgtagttct
     4381 tgctttggaa aacagtagaa acatctgtga actttctttg cacgcatcca aatttaaact
     4441 tcaaagtcag gcggaagaaa tggatatgta tcttgccatc gataacgccg tagaaaaaat
     4501 ggaagcacag ataaagaaac taaaggatcg cattacggat caccagaaac gtgctttaaa
     4561 acaacagttc gaaacatact ctcatgccac cgattttgag ttggaaccaa agagtcatat
     4621 gcgtaaaact ataaaaacta aacgcgtagt aactgaatcc ctcagtttgt ctgaagccgt
     4681 agaaaagctg gaggcaagta aagaggattt cttgattttt aaaaacatcg aatctgatag
     4741 aataaatgtt ctggtaacaa aagaccataa tgtttataag ttgctggaac catagttatt
     4801 aattgctgaa tcggataatc aggaaagagg aagagcaaaa tcaaggacta ataaactcgg
     4861 ctgtataatt atacttcggg tttaacgcta taaatacata attatgcttt tcctcttttc
     4921 ctttaagaaa atagataaaa tgtaatatgc cacacattat ttgctggctt ttaaatttag
     4981 gcaaaaccga ggtacatcaa tgaaggaaat taccgtttat gatctttttg atactaagaa
     5041 aaaagacctg gctttatcat taattaccga accggaaact ttgaacaaga aaatcggttc
     5101 cccttatctt aatcgtcccg gtttagctct ggctggttat ctggaattgt ttgcagccga
     5161 tagaattcaa gttttagggg aaaccgaagt tcgctattta caaagcttaa cggaagaaga
     5221 tttaatcacc cgcatccgaa atatgtttaa aatggatatc ccctgtattg taatctccaa
     5281 gggtcttact ctgccaccag ttattgaata tatagctaat gaactaaata ttgctctgtt
     5341 ttccagtcga ctctctacaa ttaatctaat cgaaaatcta tctcgctacc taagagatat
     5401 ttttgccctg gaaaaaacaa ttcatgcaac tctcgtagat gtatttggct taggaatttt
     5461 gctaacaggt aaaagtggca tcggaaaaag tgaatgtgct ttggatcttg tacatcgtgg
     5521 acacagctta gttggtgatg atttaataac cctgcgctat atagaggata agctgattgg
     5581 aaaaccagga cgcgaatttg gctattttat ggagattaga ggtgtgggat tcataaatgc
     5641 agaacgcgtt tttggaattg aacgcactcg taaacaaaaa accatagacc tccagataga
     5701 actgatgccc tggcaggaaa atatggatta tgaacgaatt ggtttggtta gtaattatgc
     5761 tgaacatctg ggggtgaaaa taccaatcat ttacttacct gtttctcccg gaaaaagtgt
     5821 ttcagttatt gtggaagtag ctgcaatgaa tataattctg aaaggtgttg gctatgatgc
     5881 agcagaggac tttaaccgca aggtaaacga agaaatccgt aagaaaacaa tgctgaaaaa
     5941 cattgaaggc aaaagtgatg aaacagatag aggtgaccgt aaccaataaa ttgggtttgc
     6001 atgccagacc ctcagctctc ttagttaaag ctgctacaaa atatcgcagc gattttttta
     6061 ttgaaaaaga aggaatgaga ataaatggta aaagcattat gggagttatg atgctggctg
     6121 ctgaatgcgg ttctaaaata aaacttatcg ctgatggagt tgatgaggat tatctgctgg
     6181 aagaaatcaa aacaatgatc gaaagcggat ttggagaata aatgcaaaaa atatatggaa
     6241 catccttgaa taccggtatt tttattggca acgcattgca tattgctttg cccgaaccgg
     6301 ctattgaaac aggaaatatc aggcaggaag aaatcccaaa agaaatatcc cggttgcaat
     6361 catctctttc tattgtggaa gaagatatca atctggaatt aaaaacaagc aacctgcaag
     6421 ctcaggatgc tgaaatcatc tctgctcaaa gagagatttt acgcgatccg gaaatctata
     6481 atcaaatcga aacagccatt cgcaataaat tacaatatgc tacccaagcg gtttctgagt
     6541 gctttgccaa tattattaaa cactttgaaa ctatgaaaat agaaaccttt gctgttcgcg
     6601 ctgctgatta tcgggatgtg gctatgcgcc tattaaatgc cctatcagga gttcgaaata
     6661 ctctaaaaat caaaattgaa ccaaatcaga tccctgtttt tattgaaatt gtcccttctt
     6721 tagttagtaa acttgcccaa gaaggaatta aggcatattt ggtggccaaa ggaagctaca
     6781 attcacattc ttctatttta tgccgggctc tgggattggt ggctattgct aatattccgg
     6841 atttaaatgt ggcagtgaaa aataacgact tgctgattgt tgatggctat actggattgc
     6901 tgatcgttaa tcccgatgac caaaccttag aatattacag aaaaaaagct gaagcagaac
     6961 ggcatagaca ggaaaaactg aaacagctga agataactga ttccataacc gctaatggga
     7021 ttaatattga gctgtttact aatataggcg taccagaaga attggaagtg ataaaaaacc
     7081 tttcctgtgc tggtattggt ttgtttagaa ctgaatttct ctatctggga aggcaaaatc
     7141 ttccggatga agaagagcaa tttgctgtat ataaagaact tgctgaaaaa atgaaacctt
     7201 tgcctgttac catccgaacc tttgatttgg gaggagataa actctccccc ttgggttatg
     7261 aaactccgga agaaaatccc tatctgggta atcgcggaat ccgattttca ctttcccaac
     7321 cagatatttt ttatactcaa gtgcgtgcta tcttaagggc ttccgctttt ggaaaagtgc
     7381 aaattatgtt tcctatgatc attgataccc aagattttat cgaagcaaag aaattcgtgc
     7441 aaaattgcca gcgggaactt agccaacata gtatatcctt tgatccagaa attgccttgg
     7501 gggctatgat agaaataccc tccgcagcat tatgttctga agaattagcc aaagagtgcg
     7561 attttttcag tatcggaaca aacgaccttg tacaatatac tttggctgca gatcgtaata
     7621 atgaaatggt ggccaaatat tatattcaac accatcctgc tgtgctttgc ctgataaaga
     7681 aaactataat tagtgcagaa aaatatcata ttccgctttc tgtttgcgga gaaatggctt
     7741 ccaactataa gtatattccc cttttaattg gtttgggaat tagagaatta agcgttaatc
     7801 ctggtagctt cttttccgca aaagccattg tacagaaatg cgatgcaaaa ctttttaaag
     7861 cgactgaaga aattaattgt tccactccgc tttccgaagt agagaactta ataaataata
     7921 ttttagctcc atattatcag gacaaggagg aataagtgct gaaatatgaa tatcacaatc
     7981 tattagcttc caaacagatg ctgagtattt tgcccaaaga taaaattttc gatagcgaac
     8041 atcaggttaa aaatatccat gaagaaatta tggctgaacg ccgggctaag ttacttggtt
     8101 tttatgattt accggaaaat tctaccagac atattgatga ctttatagct aatttggatc
     8161 cggtttttga tactatgtta gttttaggca ttggcggttc cgctttaggt aataaagcac
     8221 tatattcagc tttgaaaacg gaggcagaat tacctcgtaa gcttgttgtt tatgataatg
     8281 tggatcctgt tttcttatat gaaatcttgt cgcaaataaa cctggaaaca actttggtaa
     8341 atgtgatcac caaaagcgga acaactgctg aaacaatggc tggctatatg attgtggctg
     8401 atatattaaa acgcaagttc cccaaagact atcaaaagag aattatcgtt actacagata
     8461 aagaaagagg ctttttgcgc caggttatta aaaaagaagg ctataaaagc tttaccgttc
     8521 cagataatgt gggaggcaga tttagtgtcc tttccgatgt gggtttagtt tcatctgctt
     8581 ttactggcat taatattcag gaattgctaa gtggagcaaa agctatgagg gaacgctgtt
     8641 cggaaccaga tattatggag aatccagctt acttgaatgg tttgattcat ttcttatatt
     8701 atcgcttggg aaaaaacatc agtgtaatga tgccttatag taattctttg tatgatcttg
     8761 ctgattggta tcgtcaactt tgggcagaaa gtttgggtaa acgcaaagac ataaaaggta
     8821 gagaagtata tgtggggcaa acacctgtga aagcattagg aacaaccgat caacattcgc
     8881 aagtgcaact ttatacagag ggacctaatg acaaggtaat aactttcata accgtagaaa
     8941 actttaaaca tgattatata attccaaatt tgcatccaga ccacgaagaa gtaaattatc
     9001 tgggcaatag aaaactctcc gatttgctta atgcagaacg acttgctaca gaaattgccc
     9061 tcagtaaagc tatgcgtccc aatgctaata ttgttttccc ccaaattgat gaataccatt
     9121 taggagaatt cattatgctt tatgaaattc agacgatatt aacaggtaaa cttcttcgta
     9181 ttaatccatt agatcaaccc ggtgtggaat ctggaaaaat tgctacctac gccttgatga
     9241 acaaaaaggg ctacgagaaa gaacatgccg aaattgaaaa atacaaacaa gaccgtgaca
     9301 taacctaata ggtatattag ttcattgatt tacaaaaaaa gagatgaaaa atagaatctt
     9361 gaataagagg gtaaaaagct accctgttat ggaggcggat atgattttta aaagcaaaaa
     9421 ctatgattat gaaaataata ttgataggaa ttggcaaaat gaaaataatg caattgtcgt
     9481 aagacacaat gtagaactta agaactgagg ataatgagaa tgttcttaaa acaacttgaa
     9541 aaataacgag ttggaattta cactgatact tgggaatcaa ctaaattaat tttgcagagg
     9601 gatccctagt tagaaaaaac ttgaccgaat tcatagaaga gaaaacaagg acaaattaat
     9661 atttatacta taaaggagtt ataagtgaaa gctatcctaa caatagcgtt tgtactgttt
     9721 tgcatttgcc tctcagcagt ggattacatc gatgtggtct acactaaaaa tggagatgtg
     9781 ttaaaaggta tcattattga gaacattccc aatgacaaga tcaaagttga tatcaatggt
     9841 gttgtagtaa ctgttatgta cgatcaaatc ataaaacttg caaaagaaga aattgatacg
     9901 acatctacaa aagtcacaaa aagaaatgcg ataagtaaaa ctgttaatga aaatgactca
     9961 gatctaccct ttcacaaaaa atacggttta cttgggggtg tgaattcagc aaccgctaaa
    10021 gttgataatt ccgagggtgt tgatatatcc tcagtatggg gattttcttt tggaggtttt
    10081 gctaccttcc agacgagtcc gaatataatc attcaacctg aattaatcta tactggaaaa
    10141 gggtttaaaa cagaaattaa tttagatgat tatgattacg aatataaaga aaccgacgaa
    10201 tggaaatttg cctatttgga agttcctgta ttgttcaaat atgtcttcca aacaggaaat
    10261 atgtatatca ctccttttat tggaccatca cttagtatcg caatgagtgg gaaagtttct
    10321 tatgattcgt atcaatatta tcaaaattat gaagaagaag attcgggttc tatggacatt
    10381 aaaaatcaga tggaagatat tgatttcgga ctgaattttg gaactgacct gtatttctcc
    10441 gaaaagattt ttttgcaact caagtatgat ttaggtcttt caaagatttt tactaaaagt
    10501 gttttagaat caaaaaatag ggtcttgagt ttaagtatgg gcgtttcttt ctaaaaaata
    10561 cagatataag ttcattaata gaaagagatg aagaaatcga tgttttaggc tataagaatg
    10621 cttgatatag taatacatac atttcagtaa agaattgtgt ctttgactct aaaccgatcc
    10681 ttcttgaatt tgcaacgaga agcagaggca
//
LOCUS       DAOSTH010000056        10623 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_6085_length_10623_cov_18.287282, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000056 DAOSTH010000000
VERSION     DAOSTH010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10623
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6085_length_10623_cov_18.287282"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            337..5463
                     /locus_tag="PLB65_06495"
     CDS             337..5463
                     /locus_tag="PLB65_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HOQ78214.1"
                     /translation="MKELTFKFSFTLFLLCLCGFLMASVAEYSFSSSLGSFSEITNGT
                     VLGSNLNDNECFLSIPIGFTFSYNGNSYTTISIASNGFLAMGDNVTTSNVALSSETAT
                     NNVIAVMNRDLISRDNGSLMTKTIGTDSNRIFIVQWMHYRRRPTATLNDDLSFQIQLY
                     EGSNQVVFVYGPCTAVTSSTSQSIQVGLRGNSNTDFCNRATATDWSATTAGTENNSYC
                     ILSETVFPANGLTFTWNPPSLIEPPFPAVNPFPADLAENVEINTMLSWNSGGGIVNGY
                     KLSLGTNYPPTNIVYNEQLSLPQYNPGTLYYGSTYYWQVIPYNNEGGDAINCPIWQFT
                     TKQAPPMLSGTVTWAYNNLPQMNVPVKLYDASGNTELQSCLTNELGEYFFEVSYQTYH
                     LKVMAQDIPVNETNVRINAIDNVISITEDTIVDLPLEVYIPPLNTSNCTFVGIYQGHE
                     YWRSNWSAYWPDMNNEFNTRGGHHICISSEGESEFINQYIRQVTGNFHLGYVYNNGTW
                     SWIDGSPVGYTNWAPGQPDGNSEASRIYYESNSGNVYWDDVYYWEYISGIMECNYLTQ
                     PYIPLPVSSFTVQQLRDFSLSLSWVNPTQVLCGLDIADFSIIITRDGEIVQTFTNCLG
                     GQTMNWIDYQLTGQHTYGIYAVNEYGQSESIFQKVQFGNQVQGIALLNNTSNHSGIKV
                     KFIADPATPAAVTDSTYTNSNGFYSINLVIGRYNVIYSKAGYLDASYYNYLHNQPTTL
                     PEQTLEYVGQIMNLSGPLSGTITNEYTYIITGQISVDNGNSLTLQPGVKFYFKNGIGF
                     DVNGSLTAIGTVQSPIVFSSIGSEVRGDMWLRNANIQMEHCEFTKGNYGVRINAPVHI
                     SYSKFHQNNTAVYFDYNSGSNCLIDYCEVYNQNENCFYSYSNDPVKIEHCEIHSNQLT
                     GYILYISYFYKSAAFNNNYVHHNSCYSAFYLEIYCVQFILTHNIIEYNTASDRIIYVS
                     NGYRYTHIDISDNIIRYNMGSDLIYISYGSGEFKHNQIYNNSGSVQFSTSEEQYNNYG
                     TISIENNTIVGNSGTGLDITYKYTIKNNIVACNGGSELVYNSNHPALQYNLFYDTSGT
                     IFQNPGGLLFLLDLQAQNANGTPCDPYSNISLDPQFFDSANNNYNLMPTSPCINAGDP
                     NSPFDPDGTIADLGALYYDLIASSHSQIAIPMSMYNFQGTPVGETFIWNCPVRNTGTE
                     DLIISSIELSNPVFFLAVPDKSTTPFSLTVAPNATGYIPIGFTPLAVTDYADTLRINS
                     NALMNPQVSIRIFGSGVLASSVILALPQNNTVNVLSDFQLPLSISDTTPYNIISYNMS
                     VTYDPALLEFVALSTEGTLCADWYVAVNPQGNGVLYIGSSGVNPLTGSGVLFKLNFHT
                     LSGIPDNTETYINIPQIIFNEGGLTVQGCFAPVVIRNIIYGDVDDNQLIQAYDAALTL
                     QYSVMMDPLPNIDPRPWVEWRKLRADVSGDGNIYAYDASLILQRVVGLISSFPVEDQT
                     RVTPVATVNVTYYNGELVLTSPDFKVLYSLNLSLELPQNAETGQPVFSEPFSSALWNQ
                     FTENNIWNFAFACITPSEGNGTICTIPISLVNSTTLALNLVINETPTTFTLECQPTGV
                     QDLVAGTNFLAQNRPNPFNPSTTLYFGLKENAQVELSIYNLRGQKVITLVNKELNAGN
                     HSVVWEGKDATGNYVSSGIYFSRLKIGNSWSKTQKMMLLK"
     gene            5482..8292
                     /locus_tag="PLB65_06500"
     CDS             5482..8292
                     /locus_tag="PLB65_06500"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOQ78215.1"
                     /translation="MKKLIIFMLLACSCLFLLADIEDIQLDIGEQNIILGEQTTLPVQ
                     TSSLSGYGVISYELNIAYDTQKLTFLEIVKTNTLSANGMISVNEVNGILHIGAIFTNP
                     LYGQGNLLNLKFSAQNPGLAEVNFVSAVMNTTSLMNLYGGSIYIENQPISASLSVGNY
                     LLQTGSSFSLPIIATGLAGMNCISFQFKFRFDPAVINVISVNNSGSSSEGWEINNRTS
                     SGLISVAGMGTMPITADGTLIFIDIEVLPTAITFSHLQLETVLFNTQPAVSVVDGRLD
                     VYNLWNLHIEGESDIMAIHNNNPLFIWNVINSSATPFECEFQAGSDDNWEIAEYYASG
                     WIENYTSQQLLNLVYGTALFARIRLRIGDFISPWQELAGHLIPLPGSAVCLSPLPNAL
                     NVILNPVLSWQSGLGDPPLGYRLYFGTNTPPSNIVNGTDLGLVTTWQPSSPLQYNTVY
                     YWQIIPYNTYGEPSSCPIWSFTTHDANAIVQYPYLKDFYDGSVTSGGWTTFYSGSAGS
                     NWRINTTNGISGSKCITVGRAPGTYWYISPAVINPNCGSALSFYIKDYSSNSTYDIVG
                     ENLEIMLSTTGSTPQNFTHCLLSLDNFAVTTSYISFTVDLTQFASQSIYLAFKRVSNN
                     GNYLFIDNIQLEQYIPQITLNTSVLVFPTLKPGQTANANFSISNSGTGILSGNLIYPE
                     GFFGVNSFSGNSNTIEVSYHPWSTGGGSYPLQITSSGGNTILYLQANCGEEIQPCESL
                     TNSGYIDNNWQLSSAEKHNGSSSWTTISPEMYLISSWMQGGNGKGFGFWAKANSASSI
                     AIYLNSTGYAIEQNYLYHSNIPLDNNWTYHRISFSDYIDLSVSVKLVCDSGIFIDDID
                     MSNFLAPQTPEVLITKTETVPTISWLPTFNCLSYNVYASSNLTDWSLISPANFQETLW
                     VDNSQLEHRFFKVCGVNGKISE"
     gene            8763..>10623
                     /locus_tag="PLB65_06505"
     CDS             8763..>10623
                     /locus_tag="PLB65_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78216.1"
                     /translation="MRKSLFLILLTLSFIGGLFAQVNIQSGQNVTQNFDVLGTSAAAT
                     IPTGWKADKNTTVRSVGTYSAAGTVTELIAGNNMSSTAQNGIYNYGAGPAATATDRAI
                     GFISSGSATKSGNVYVQLTNNGSTNINSFSISYNVEKYRMGTNAAGFSIQMYYSANGA
                     TWTSAGSSFLTSFPADATTAGYDSAPGVTVPVSATLSYSLSAGSSLYLAWNYSVTNGS
                     TTSYAQGLGIDDVSITAGGAPTPVIVVNGNLTPFSTYTGTPSTAQNYTLSGQNLTGNI
                     TVAAPTGFALSTDGTTYTSNLSLPATFNGLVYVRLTGAAAGNFSGNIVHSSTGATNVN
                     LAVEGTVTDPTPTINITANLSPFGTQLGTPSAAQSYTVTGLFLTAGISVTAPEGFALS
                     TDNTTFTNTLTLASNFSGLVYVRLTGATAGTFSGNIVHSSTGAETQNLAVSGLVIDPN
                     VATTLLEEEFIYPAGDLLTAHGWFAHSSPGIHPVIVANEGLIYPGYYAYQGLAAQTVL
                     SGAEDVKKTFGDQTSGTFYVSFLVNASEVKTAADYFFHFLPSSSSSTDFKGKVFVQRD
                     ASNNLRFGITKASSAISESAATTYSYSLNTTYLIVVKYVIVSGATNDEVYMWVN"
BASE COUNT         3098 a         2009 c         2052 g         3464 t
ORIGIN      
        1 gataaattaa aattatcata aaatctcggt gttttcggtg tataaatata atctcctttc
       61 ctccttttct ctttgtaaaa aatctctttt cctccttctc tctttgtaaa ataaatctct
      121 tttcctcttt ttctctttgt aaaaaatctc tttgttttcg gtgctttcgg ggtaaaaaat
      181 cacccgttga taaaatttag ttgacagaaa attcaggttg attttttaat gtaagtatgt
      241 ttaatcagtt tgttttgcaa cattggaata ttttatagtg tgtaagcaac aataataagg
      301 gaatcaacaa tcattaaata gaggagaata acaaaaatga aagaacttac tttcaagttt
      361 agctttacct tgtttttact ttgtttgtgt ggctttctga tggcttccgt tgcggaatat
      421 agctttagct cttccttggg aagtttttca gaaataacga atggaaccgt tctgggctcc
      481 aacctcaatg acaatgaatg ttttctttct attcctatag gatttacctt tagttacaat
      541 ggcaattcct atactacaat aagcattgct tccaatggtt ttttggctat gggcgataat
      601 gttaccacca gcaatgtagc tctcagtagt gagacagcta caaataatgt aattgcggta
      661 atgaatcgtg atctcataag tcgtgataac ggctcgctga tgacaaaaac tattggaacg
      721 gactccaacc ggatatttat tgtgcaatgg atgcactata gaaggagacc aacagccacc
      781 ctcaatgacg atttaagttt tcagatacaa ctttatgaag gcagtaatca ggttgttttt
      841 gtttacggac cttgtactgc tgtaacttct tctactagcc aatctatcca ggtgggctta
      901 agagggaata gcaataccga tttttgtaat agagctactg ctactgactg gtctgccact
      961 acagctggaa cagagaataa cagttattgt attttaagtg aaacggtttt tccagccaat
     1021 ggattaactt ttacctggaa ccctccttct ttaattgagc caccctttcc agcagtaaat
     1081 ccttttccgg ctgatttagc ggaaaatgtg gaaattaata caatgcttag ctggaatagc
     1141 ggaggtggaa ttgttaatgg ctataaatta tctttgggca caaattatcc tcctacaaat
     1201 atagtataca atgaacagtt aagcttgccg caatacaatc ccgggacatt gtattatggg
     1261 tcaacttatt attggcaagt gataccttac aataatgaag gtggtgatgc cataaattgt
     1321 ccgatttggc aatttacaac taaacaagca cctccaatgc ttagcggaac tgtaacctgg
     1381 gcttataata acttgcccca gatgaatgtt cccgtaaaac tttatgatgc cagtggcaac
     1441 acggaactgc aatcctgcct tactaacgaa ctgggagaat atttctttga agttagttac
     1501 cagacctatc atctgaaagt gatggcacaa gatatcccgg ttaatgaaac caatgtgaga
     1561 ataaacgcta ttgataatgt aatttctata accgaagata ccatagttga cctcccttta
     1621 gaggtctata tccctcctct taatacttcc aactgcactt ttgtaggtat ttaccaaggt
     1681 catgaatatt ggcgtagtaa ttggagtgca tattggcctg atatgaataa tgaatttaac
     1741 accagaggcg gacatcatat ctgtatcagc agtgaagggg aaagtgaatt tatcaatcaa
     1801 tatataaggc aggtaacagg aaattttcat ttgggctatg tatataataa tggcacttgg
     1861 tcttggatag atggttctcc tgtgggctat acaaattggg cacccggtca gccagatggc
     1921 aattcagaag ccagccgtat ttattatgaa agtaatagcg gtaatgtcta ttgggatgat
     1981 gtttattatt gggaatatat tagtggaata atggaatgca attatttaac tcaaccttat
     2041 attcctttac cggttagttc tttcactgtc caacaattga gggatttttc ccttagccta
     2101 agttgggtaa atcctaccca agtactctgt ggattggata tagccgattt ctctattata
     2161 attacccggg atggagaaat tgtgcagacc tttaccaact gcctgggtgg acaaacaatg
     2221 aattggattg attatcaact taccggtcag catacttatg gaatttatgc cgtaaatgaa
     2281 tacggtcaaa gtgaaagtat tttccaaaag gtgcaatttg gcaaccaggt gcagggtatt
     2341 gctctattaa ataataccag taatcattcc gggatcaaag tgaaatttat tgccgatcct
     2401 gctactcctg ctgcggtgac agatagcaca tatacaaact ccaatggctt ttacagtatt
     2461 aatctggtta ttgggcgtta taatgtcatt tattccaaag caggttattt agatgcttcc
     2521 tattataatt acctgcataa ccagcccact actttacccg aacagacctt ggaatatgta
     2581 ggacaaataa tgaatttaag cggaccttta agcggcacga taaccaatga atacacttat
     2641 attattaccg ggcagatctc tgtggacaat ggaaattctt taactttgca accgggagtg
     2701 aaattctact ttaaaaacgg tatcggcttt gatgtgaatg gcagtttaac tgctattggt
     2761 acagtgcaaa gccccattgt tttttcctct attggctctg aagtgagggg agatatgtgg
     2821 ttgaggaatg ccaatattca aatggaacac tgtgaattta ccaaggggaa ttatggagta
     2881 agaataaatg ccccggtgca tatttcctac agcaaatttc atcagaataa tacagctgta
     2941 tattttgatt ataattcagg atcaaattgc ttgattgact actgtgaggt ctataaccag
     3001 aacgaaaatt gtttttattc ttatagcaat gatcctgtta aaattgagca ttgcgaaatt
     3061 cacagtaatc aactgacagg ttatatccta tatatttctt acttttataa atcggcagca
     3121 tttaataata actatgtcca tcataacagc tgttacagtg ctttttatct tgaaatttat
     3181 tgtgtacaat tcattttaac ccataacata atagaatata atactgcctc tgatagaatt
     3241 atctatgtta gtaatggtta tcgttataca catattgaca tatccgataa tattatccgc
     3301 tataatatgg gttcagatct gatatatatt agttatggct ctggggaatt taaacacaat
     3361 cagatttata acaatagtgg ttccgtacaa ttcagcacat ctgaagagca gtataataat
     3421 tatggtacca tatccattga aaacaatact attgtgggta atagcggaac tggtttggat
     3481 attacatata aatataccat caagaataat attgttgcct gcaatggggg ttcggaattg
     3541 gtgtataact ccaatcatcc tgctttgcaa tataatctct tttatgatac cagtggaacg
     3601 atatttcaaa atcccggtgg cttgcttttc ctgcttgatt tacaagcaca gaatgccaat
     3661 ggcactcctt gtgatccata cagtaatatt tcgctggatc cgcaattctt tgattctgct
     3721 aataacaact acaatctaat gcctaccagc ccttgtatta atgccggtga tcctaatagt
     3781 ccttttgacc ctgatggcac gatagctgat ttgggtgctt tatattatga tcttattgcc
     3841 tcgtcgcatt ctcaaattgc cattccaatg agtatgtata acttccaggg aacgccagtg
     3901 ggagaaactt ttatttggaa ttgtccggtg agaaatacag gaacggaaga cctgattatt
     3961 tcttccatag aactgagcaa tccggttttc tttttagcag ttcctgataa gagtacaact
     4021 ccttttagtt taactgttgc tcctaatgct accggttata ttcctatcgg ttttactcca
     4081 cttgccgtaa ccgattatgc cgatacatta cgcattaata gcaatgcctt gatgaatcca
     4141 caggtctcta tccggatttt tgggtcggga gttcttgcca gcagtgtaat tctggctttg
     4201 ccgcaaaata atacagtgaa tgtgctctcc gattttcaat tgcctctttc tatttcggat
     4261 accacccctt ataatattat ctcctataat atgagcgtaa cttatgatcc cgcacttttg
     4321 gaatttgttg ctctcagcac cgaagggact ctttgtgcgg attggtatgt agctgttaat
     4381 ccccaaggca atggtgtcct ctatattggt tccagcggag ttaatccgtt aacgggaagt
     4441 ggagttcttt ttaaattgaa tttccatact ctttccggaa ttccggataa cacagagacc
     4501 tatattaata ttccgcagat tattttcaat gaaggcggtt taaccgttca gggttgtttt
     4561 gctcctgttg ttattcgcaa tattatttat ggcgatgtgg atgataatca actgattcag
     4621 gcatacgatg ctgctttaac tttgcagtat tcagtaatga tggatcccct acctaatatt
     4681 gatccacgtc cttgggtgga atggcgaaaa ctgagagcgg atgtttcagg tgatggcaat
     4741 atttatgctt atgatgcttc gcttatttta caaagggtag tgggtttaat tagcagtttt
     4801 ccagtggaag atcaaacgcg ggttacccct gtggcaactg taaatgttac ttattataat
     4861 ggagagttgg ttcttacttc tcctgatttt aaagtccttt acagtttgaa tctctctttg
     4921 gaactgccgc aaaatgcaga aaccgggcaa cctgttttct ctgaaccttt ctcttccgct
     4981 ttgtggaatc agttcacgga aaacaatatc tggaattttg cttttgcttg cataactccc
     5041 tctgaaggca acggcactat ttgcactata cctatttctt tggttaattc cactactttg
     5101 gcattgaatc tggtaataaa tgaaaccccc actactttta ctttggaatg tcagcccact
     5161 ggagttcagg atttagttgc gggaacgaat ttcctggcac agaatcgtcc taatcctttc
     5221 aatccttcta caacgctata ttttgggtta aaagaaaatg cccaggtaga gcttagtatt
     5281 tataacctaa gaggtcaaaa ggtgatcact ctggtgaata aagaactaaa tgccggtaat
     5341 catagtgtag tttgggaggg aaaagatgct acgggaaatt atgtaagcag tggtatctat
     5401 ttcagccgtt tgaaaatagg caattcctgg agtaaaactc aaaagatgat gttactgaaa
     5461 taagctttag gagctgttat tatgaaaaaa ctaataatct ttatgctcct ggcttgtagt
     5521 tgcctctttt tactggcgga tattgaagat attcagctgg atattggaga acaaaatatc
     5581 atcttgggtg aacagaccac tttgccggtt cagacatctt ctttaagcgg atacggagta
     5641 atcagctacg aactgaatat cgcctatgat acccaaaaat tgacttttct ggaaatagtg
     5701 aagaccaata ctctttctgc caatggtatg attagcgtta atgaagtaaa tggaatttta
     5761 cacattggag ctattttcac taatccttta tacggacagg gcaacctttt gaacttgaaa
     5821 ttcagtgctc aaaaccctgg tttggcagaa gtgaattttg tttctgctgt aatgaataca
     5881 acatcgctta tgaacttata tgggggaagt atctatatag aaaatcaacc tattagtgct
     5941 tccctctctg tagggaacta tctcctgcaa acaggaagtt ctttttcgct tccaattatt
     6001 gctacaggac tagctggtat gaattgtatc agttttcagt tcaagttcag gtttgatccc
     6061 gctgttatta atgttatcag cgtaaataat agcggtagta gcagtgaggg atgggaaatt
     6121 aacaatagaa cttccagcgg tttaatttct gttgctggaa tgggaactat gcccataact
     6181 gcggatggaa cactaatctt tatagacatt gaggttttgc ctactgctat aacttttagc
     6241 catctccagc tggaaactgt tcttttcaat acacaacctg cagtaagtgt tgtggatgga
     6301 cgattggatg tttataatct ctggaatttg catatagagg gggaatcgga tattatggct
     6361 atccataata acaacccgct ctttatctgg aatgttatta acagcagtgc aacgcccttt
     6421 gaatgtgaat tccaagccgg aagtgatgat aattgggaga tagcagaata ttatgctagt
     6481 ggctggattg aaaattacac cagccaacag ctgcttaact tggtttatgg aacagctcta
     6541 tttgccagaa ttcgtttaag gattggtgat ttcatttctc cctggcaaga actggcaggg
     6601 catctaattc cactacccgg ttcagctgtt tgcctcagtc ctttgcccaa tgccctaaat
     6661 gttattctca atcctgtttt aagttggcaa agtggacttg gtgatccacc tcttggctac
     6721 cgactttact ttggaactaa tacacctcct tccaatattg taaatggaac agatttagga
     6781 ctggttacta cttggcaacc ttcttcgcca ttacaatata atactgttta ttattggcag
     6841 ataattcctt acaatactta tggagagcca agttcctgtc caatttggag ttttactact
     6901 catgatgcca atgctattgt ccagtatcct tacctgaaag atttttatga tggaagcgta
     6961 acttccggtg gctggactac tttttatagt ggttctgcag gtagtaattg gcgaataaat
     7021 actaccaacg gaatttctgg aagcaagtgt ataacagtag gaagagcacc tggaacttat
     7081 tggtatatta gcccagcagt aataaatccc aattgcggtt ctgccttaag tttctatatt
     7141 aaggattatt ccagcaattc aacttatgac attgtgggtg agaatttgga gataatgctt
     7201 tctactactg gttcaacacc tcaaaacttt acccattgtc tcttaagttt ggataatttt
     7261 gccgttacta cctcttatat atctttcact gtggacttaa cccaatttgc cagccagagt
     7321 atctatcttg cctttaaacg ggtttccaat aacggtaatt acctctttat agataatatt
     7381 caattggaac agtatattcc tcaaatcact ttgaacactt ctgttttggt ttttccgacc
     7441 ttaaaaccgg gacaaacagc taatgcaaat ttcagtataa gcaatagcgg aacaggtatc
     7501 ttgagtggaa atttaatcta tcctgagggc tttttcggag tgaacagttt cagtggaaat
     7561 agcaatacta ttgaagttag ttatcaccct tggtcaacag gaggaggtag ctatccctta
     7621 caaataacct ccagtggagg taatacaatt ctttatctcc aagccaattg cggagaagaa
     7681 atacagccct gcgaaagctt aaccaattcc ggttatatag ataataattg gcaactctca
     7741 tctgcggaaa aacataatgg ctcatcttcc tggacaacta tttcacctga aatgtatcta
     7801 atcagctcct ggatgcaagg tggtaacgga aaaggtttcg gtttctgggc taaagctaat
     7861 tcggcaagct caatagctat ttatttgaac agtaccggtt atgcgataga acaaaactac
     7921 ctctatcact ccaatatccc tttagataat aactggactt atcaccggat ttctttttct
     7981 gattatatag atttaagtgt ttcagtaaaa ttagtttgtg attcgggcat ttttatagat
     8041 gatatagata tgagcaattt tcttgctcct caaacgcctg aagttttaat taccaaaaca
     8101 gaaacagtac ccacaatcag ctggcttcct acctttaatt gcctaagcta taatgtttat
     8161 gccagctcta atcttactga ttggagttta ataagccctg ctaatttcca ggaaacactg
     8221 tgggttgata attcacaatt ggaacataga tttttcaaag tgtgtggagt gaacgggaaa
     8281 ataagtgaat aagttaaaaa atagcgaggt ggtgacataa cgagatggtg agattgtaaa
     8341 cggtgaaaag ttaaaagtga acggtgaatg gagtatggag aaagaattaa gtcaggagtc
     8401 gtcattgctt gtataactat atcgctttct tcaaattagt ttagcaatga ggactcttga
     8461 cgacctgtga ataaagatag ttcataaatt ataaagtaat atcggtgctt tctgtgttct
     8521 cggtgtttaa aaaatctctt ttcctctttt tctctttgta aaataaatct cctttcctct
     8581 ttctctcttt gtaaataaat ctcggtgccc tcggtgttaa taataatcag cagcatctga
     8641 acaaaaataa aagaacatat tattttactt gacgccatac ccttcttcaa atattaaaca
     8701 ctttacgagt aattaatata tctgattata agttaggaac taagttttct caaggaggaa
     8761 atatgagaaa gtcattgttt ttgattctac taaccctttc ctttatagga gggttattcg
     8821 ctcaggtaaa tattcaaagt ggccagaatg taacccaaaa ctttgatgtt ttgggaactt
     8881 cagctgctgc aaccatacct accggttgga aagctgataa aaatacaacc gttagaagtg
     8941 tgggaactta ttctgctgct ggtactgtca ctgaactaat agcagggaat aatatgagtt
     9001 caactgctca aaatggcatt tacaattatg gggcaggtcc agcagctact gctaccgata
     9061 gagcaattgg ttttatatct tccggttctg ctaccaaaag tggaaatgtc tatgttcagt
     9121 taaccaataa cggcagcact aatatcaatt ctttttccat aagctacaat gtggaaaaat
     9181 accggatggg aactaatgca gccggttttt ccatacaaat gtattattct gcgaatggag
     9241 caacctggac ttctgccgga agttcatttt taacctcttt cccagctgat gctacaactg
     9301 ctggctatga ttcagctcca ggggtaacag ttccagtatc tgccacttta tcttactctc
     9361 tttcggcagg aagctctttg tatcttgctt ggaactattc cgtaacaaat ggttcaacca
     9421 cttcctatgc acagggcttg ggaattgatg atgtaagcat tactgctgga ggtgcaccca
     9481 caccggttat cgtagttaat ggcaatttaa ctcctttttc tacttatact ggtactcctt
     9541 ccacagctca gaattacact ttgagtggtc aaaatttaac cggaaatatc acggttgctg
     9601 ctcctacagg ttttgctctt tctactgatg gaacaaccta taccagcaat ttatctttac
     9661 cagccacttt taacggttta gtatatgtac gcttaaccgg agctgcagca ggaaattttt
     9721 ccggcaatat tgtgcattcc agtaccggag caactaatgt aaatcttgct gtggaaggaa
     9781 ctgttaccga tcctactcca acaattaata ttacagctaa cttaagcccc ttcggtacac
     9841 aattgggaac tccttctgct gcgcaaagct atacagtaac cggattattt ttaactgccg
     9901 gaataagtgt tactgcacct gaaggttttg ccctttctac agataatact acctttacca
     9961 acactcttac tttagccagt aatttcagcg gacttgttta tgtacgttta acaggagcca
    10021 cggcaggaac tttctccggc aatatagtcc attccagcac aggagcagaa acccagaatt
    10081 tggctgtaag cggattggtt attgatccta atgtagcaac aactttactg gaggaagaat
    10141 ttatttatcc cgctggcgat ctcttaacag ctcacggttg gtttgcacat agttcaccag
    10201 gaatccatcc tgttattgtt gcgaatgaag gtttaattta tcccggttat tatgcttatc
    10261 agggtttagc cgctcaaaca gttttatcgg gtgcggaaga tgttaaaaag acctttggtg
    10321 atcagacctc tggcactttt tatgtctcct ttttggttaa tgcttccgag gtaaaaacag
    10381 ctgctgatta tttcttccat tttttaccca gttcgtcttc atcaactgac tttaagggca
    10441 aagtatttgt ccagagagat gcttccaata acttaagatt cggtataact aaagcatcaa
    10501 gtgctatttc agaatctgca gctactactt acagctatag tttaaataca acctatttga
    10561 ttgtggttaa atatgtaatt gtttcaggtg ctactaatga tgaggtctat atgtgggtta
    10621 atc
//
LOCUS       DAOSTH010000057        10322 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_6315_length_10322_cov_12.817960, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000057 DAOSTH010000000
VERSION     DAOSTH010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10322
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6315_length_10322_cov_12.817960"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(379..2559)
                     /locus_tag="PLB65_06510"
     CDS             complement(379..2559)
                     /locus_tag="PLB65_06510"
                     /inference="COORDINATES: protein motif:HMM:NF023459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HOQ78217.1"
                     /translation="MKTRYWIIPLLLMLCLLMAAKKEKYGSIYGSITEAGKNTFVADL
                     TVQCLQKNTVIATAVSDQNGFYRFDKLKPGTYTIKINDPNFAVYENKKVKIKANKPAL
                     LNIVLVANPQKPVPEKKTETGKLSITVTDNNTPLQYASVAIYDNNKRLTGGQTDAQGK
                     AVILNIPAGTYSLKCSLIGYNTVEKSNITIEAGKTTTLTITMQKTGIIMESVKVNAVS
                     DMDVVPWQTLLSASMQNQYITPFNTEQYEAIKPNIFHSPLAEPLSTFSIDVDTGCYSN
                     IRRILQQGQLPEPKSIRIEELVNYFAYNYPKPEGEHPFSVYTEMSVCPWNQNRNLVLI
                     GLQAKKLDFSKAAPSNLVFLLDVSGSMDEPNKLPLVKASMKLLVDNLRDEDKIAIVVY
                     AGAAGEVLPSTSARQKKDIYDAIDRLEAGGSTAGGAGIELAYKIAEENFIKGGNNRII
                     LCTDGDFNVGASSSAHLTQLIESKRNKGIYLTALGYGMGNYKDNTLELLADKGNGNYA
                     YIDDISEAKKVLVNEMASTLYAVAKDVKLQIEFNPAHIKAYRLIGYENRLLNKEDFKD
                     DTKDAGELGAGHCVTAVYEIIPTASKEPIPELDELKYQDIKISDEARKSPEALTVKLR
                     YKLPDSDTSIPFEVPVMNKTIHLENASENYLFACSVIGYGMLLQDSQYKAALNWDMVK
                     ELATKNIGKDTEGYRTEFLKLIDLAAKLQEKQIREENRDFNKEY"
     gene            2809..2884
                     /locus_tag="PLB65_06515"
     tRNA            2809..2884
                     /locus_tag="PLB65_06515"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2842..2844,aa:Lys,seq:ctt)
     gene            3030..4196
                     /gene="metK"
                     /locus_tag="PLB65_06520"
     CDS             3030..4196
                     /gene="metK"
                     /locus_tag="PLB65_06520"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HOQ78218.1"
                     /translation="MTAALHALDKTDRNGYVFTSESVSEGHPDKVCDQISDAILDAYL
                     AADPHSKVACETLATENRIILSGEISSKATVDIESIVRKTIQEIGYTSPNKGFDYNCQ
                     INNYLHRQESALEENTGAGDQGLMFGYACSQTQNMMPLPIELAHKLLFNLATARKKGT
                     IKHLLPDSKSQVSMRYLGRKAVDLKCLVISTHHDILTEMEFEELKEQIQQLIIIPSIR
                     DIEDGCKGYFETKNCEIKINPLGPWQDGGPAFDTGLTGRKIIVDTYGGWAQHGGGAFS
                     GKDATKVDRSAAYMGRHIAKSIVASGLADECLIQFSFVIGEQKPISLMVDTFGSGKES
                     DAEIERIIAKNFDLTVSGIIDYLNLRKPIYFPTASYGHFGRDNFSWERVKKITR"
     gene            4233..5030
                     /gene="kdsA"
                     /locus_tag="PLB65_06525"
     CDS             4233..5030
                     /gene="kdsA"
                     /locus_tag="PLB65_06525"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HOQ78219.1"
                     /translation="MSKLYTDLANSQNFFLIAGPCVIEEERIMYSIAETLKELCTRKG
                     IPLIFKASYKKANRSSVDSFSGPGLEEGLQTLAKIKQDFELPVLTDVHECIEITSAAD
                     VCDILQIPAFLSRQTDLIKNAAATGKIINIKKGQFMAPEDMQFAAEKARSTGNEQILL
                     TERGTTFGYHNLVVDFRSFTIMSQFGYPVVYDVTHSLQRPSLSNISGGNPEYAFSMAK
                     AAIATGKVKGLFIETHPEPQKAKSDAASMLSLDKMESLLDGCLQLLN"
     gene            5043..5603
                     /locus_tag="PLB65_06530"
     CDS             5043..5603
                     /locus_tag="PLB65_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HOQ78220.1"
                     /translation="MYDKRALNRPNKRVVPWNEIKLLVFDCDGVLTDGRFVYGTNNLE
                     IKNFHGHDGLGFKLLQHTDIIVCFITGRSSEALERRCLDLNITYLFQNVANKKECLDN
                     LLKDLSLTYTNVAYMGDDWNDVPVMRKVAFSAAPADAQPGIIHLADWVSEFKGGHGAA
                     RDLINYVLIHKGIYEKTILSYLDSIG"
     gene            5539..6117
                     /gene="lptC"
                     /locus_tag="PLB65_06535"
     CDS             5539..6117
                     /gene="lptC"
                     /locus_tag="PLB65_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOQ78221.1"
                     /translation="MSLFIKASMKKRFCPIWIASVSVVILLSSCQKVDLQSHTGVLAR
                     DLPDEISYKVQLTQLNNDHNEYILEAERIERFYDRRLLYAYKVTLTTFDKNNQISSVI
                     KADTTIVDDARNLIFANGNASLNSPNGTLSTPRMAWDRTVDEITAPLEVTVTRKGNVM
                     YGRNLRTNSKLSFVEMDAVSAEGYFKEEEINW"
     gene            6114..7190
                     /locus_tag="PLB65_06540"
     CDS             6114..7190
                     /locus_tag="PLB65_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HOQ78222.1"
                     /translation="MKRILSCLILFLMLFSLYAKGQADEKFRLIHSDKLFMTKAGEEQ
                     ALELNGNVHFFYGNTEFLCRNALILDTQKIARLSGNVIVKNDSLTLEADTLAYYRIPD
                     LMNLGGRITATEKTKTGIYRWIKSNFASYDKANDILTTWNRVNSFDYQENAHIQCGYA
                     QWDRKKGYAILLEEPFITSGTKDTLSVAAEKMEYFDQEHKLIATFNVKVERNEFHATS
                     DFLIYFLNEEKAIFTGEPKFFSNFADAKAMEFHLFLKDRSLMRAELIDSCHIDFAEEQ
                     FMEKKNKVDADLVTMDFLDDQLRIFVAEGNVDYYFQQDQTEKKDFMINSATGAYLQAN
                     FDDNSKLQSLQMKTDIKGKYVFQQ"
     gene            7348..8109
                     /locus_tag="PLB65_06545"
     CDS             7348..8109
                     /locus_tag="PLB65_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOQ78223.1"
                     /translation="MQIPFNMNRLKDTELRYPQRFANMLTKNYGLIFFNEGNKASQDS
                     NHAIIQDLIGVESSLRDIESFYKSKGIRPCLYPSLTQKELAVLAPLIENHNFKLVMKN
                     SEFFLHDHESSLREIDDLKIERVHKLDIDIMETIAIEYGGDWTIRVVERHLLHPSYHF
                     LGGYYKGELATMASISIFAGYTRIDDVFTRDKFRGKGFAGAMIHHLVNYHSRISPNYL
                     YVQSDLPSTIKIYEKAGFSCLPRDFDCWIAHKENN"
     gene            8414..9136
                     /locus_tag="PLB65_06550"
     CDS             8414..9136
                     /locus_tag="PLB65_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOQ78224.1"
                     /translation="MIGQRIKQIRTALGIKQVELAKVLKMNPSAVSQIESGRTNPSLE
                     TLSELGKIYNINLHWLITGTGEMFNTIAETESQSGAWDNFQKLMNERLEEIVQARKDL
                     MDSDIVEIAVSGEIAAGAPIENYGASLEVVTVSRSLINGSLSDFVALRVNGRSMEPDI
                     RNQDVVLIRLSNEWRELEGKICAIRIDGGITLKRLLLDDARKMVVLVPINEDFQPIIV
                     DPDSHSDVTLIGSLYFLFRILS"
     gene            9155..>10322
                     /gene="rmuC"
                     /locus_tag="PLB65_06555"
     CDS             9155..>10322
                     /gene="rmuC"
                     /locus_tag="PLB65_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOQ78225.1"
                     /translation="MSSIYLLLLALILLSLLNLYILLFHRRKESRTDNLNELQNAFNS
                     FEHYNREEISKLRTELLNISAENRKELNESIRAQNEVLIQQSTTSREELNNSFNALKQ
                     QFNNDAATNREELKKALNDLSDNFSRKMTELINIQQQEAENSRKTLESKMEQIRQSNE
                     TKLEQMRQTVDEKLHDTLEKRLGESFKQVSERLELVHKSMGEMQNLANGVGDLKKALT
                     NVKTRGVLGEIQLENLLEEMLTPEQYEKNFRPNKNRDEKVEFAIRLPGKSDEQDFVYL
                     PIDAKFPIEDYYRILDAYDLGDLASVENAQKSLVQKIKNCAKDIRDKYINPPITTDFG
                     MLFLPFEGLYAEVLRNTGLFETIMRDYQVIICGPTTTAAVINSLQVGFRTLAIQK"
BASE COUNT         3291 a         1821 c         1981 g         3229 t
ORIGIN      
        1 ccgctgggac ttttttatat cttttatttc gaggggctta cgccaccatc gctatttttg
       61 tgtcgttctt tgagttaaaa tcggttttgt ttgtgatctt ccacatcaat tcattctttt
      121 cccctatctt tgaataaggt cactaattat ctcatagaaa aggccgattt tgacttaaag
      181 tcgactaatc gtcagttata aaatacaatc ctctgaatcc taacaccctt aaatccttgt
      241 ttctaaagct aaatctgcgt catcagcata atctgcgtga actaatcctt aaatccttgt
      301 tccgaaaatc caaatctgtg taatctgtga gagataaaaa aaccggagga acagtgtcct
      361 ccggctaccc aatattattt agtattcttt attaaaatcc cggttttcct cgcggatttg
      421 tttttcctgt aatttagctg ccagatctat cagttttaaa aattcagttc tatagccctc
      481 ggtatctttg ccgatatttt tggttgccag ttctttaacc atatcccaat tgagggctgc
      541 tttatattga gaatcttgca aaagcattcc atagccaata acggaacagg cgaaaagata
      601 attttcggag gcattttcca agtgtatagt tttattcata accggcactt caaaaggaat
      661 gctagtatcg ctatccggaa gtttataacg caatttaacc gttaatgcct caggtgattt
      721 acgcgcctca tcggaaatct ttatatcctg atatttcagt tcatccagtt cgggaatagg
      781 ttctttggaa gcggtgggaa taatttcata aacagctgta acacaatgtc ctgcacccaa
      841 ttcaccggca tctttggtgt catccttaaa gtcctcttta ttcagcagac ggttttcata
      901 gccgattaaa cggtatgcct taatatgagc gggattaaat tcaatttgca gtttcacatc
      961 tttggctacg gcataaagag tgcttgccat ttcattaacc agaacttttt tagcttcact
     1021 gatgtcatct atataagcat aattgccatt tcctttatca gccaaaagtt ccaaggtgtt
     1081 atccttataa tttcccattc catatcccaa agcggtaagg taaatgcctt tattgcgttt
     1141 ggattcaatt aattgagtta aatgggcaga ggaagatgcg cccacattaa aatcaccatc
     1201 ggtacaaaga ataatacggt tgtttccacc tttgataaaa ttctcctcgg caattttata
     1261 ggcaagttca attcctgctc ctccagctgt tgatcctcct gcctctaatc tatcaatagc
     1321 atcataaata tctttttttt gtctagcact agtggaaggt aaaacttctc ctgcagctcc
     1381 ggcataaacg acaatcgcaa ttttatcctc atcccgtaaa ttatccacca acaatttcat
     1441 tgatgctttt accaaaggca gtttattcgg ttcatccata gaaccggaaa catccagcag
     1501 gaaaactaaa ttactgggtg cagctttaga aaaatccagc ttttttgcct gcaggcctat
     1561 taaaaccaag ttacgatttt gattccaggg gcacacactc atttcagtat aaaccgaaaa
     1621 aggatgttcc ccttccggct taggataatt ataggcaaaa taatttacca gttcttcaat
     1681 gcggatgctt ttgggctcag gtaattgacc ttgttgcaaa atacgccgga tgttactata
     1741 gcaaccagta tccacatcta tggaaaaagt ggaaagcggt tctgctaaag gactgtgaaa
     1801 tatattgggt ttgattgctt catattgttc tgtgttaaaa ggagttatat attgattttg
     1861 catactagcg gaaagtaatg tctgccacgg cactacatcc atatcggaaa cagcattgac
     1921 ctttacactc tccattatga ttccagtttt ttgcattgtt attgtaagag tggttgtttt
     1981 ccctgcctca attgtgatat tactcttttc aactgtatta taaccaatta atgaacattt
     2041 taaagaatat gtaccagcag gaatatttaa aataactgct ttcccttggg catctgtctg
     2101 accacccgtt aggcgtttat tattgtcata aatagccaca ctagcatatt gaaggggagt
     2161 attattatcg gtaacggtta tggatagctt acctgtttcc gttttctttt caggaacagg
     2221 tttttgcgga ttggcaacca gcacaatatt taataaagca ggtttattgg cttttatctt
     2281 cactttctta ttttcataaa ccgcaaagtt gggatcgttt atcttaatgg tgtaagttcc
     2341 tggttttagt ttatcaaatc ggtaaaaacc attctggtcg gaaacagcgg tggcaataac
     2401 agtgtttttt tgcagacatt gcacagtgag atctgctaca aaggtatttt tacctgcctc
     2461 ggtaatagaa ccatagatag aaccgtattt ttctttttta gctgccatca ataagcatag
     2521 cattagcagc aaaggaatta tccaatagcg tgttttcatt tttcctccat acttttatcc
     2581 attttttata gtttaatgca taatcattat ttgtcaagca attattgaag tttacctgta
     2641 aacggtttat gcgagtaaga aagaaataca gtacctaata gctaattttt taataccttt
     2701 tccatatctc tatttaccat ttttgataac tattaattac actaatttgc atcacccttt
     2761 ttgcttgaca ttatgcacct cttgaaaaag atgacccaaa ctttatgtgc atctttagct
     2821 cagctggcag agcaactgac tcttaatcag tgggtccggg gttcgaatcc ccgaagatgt
     2881 accattttta aaatagcggt gatttgccct ttgctccgca gccaaaatgt gctaaagtat
     2941 ggctttataa gttttgccgg ctttagcagc cggttttttt tagatataaa agggcaactt
     3001 aagcagataa attatattag gagatacaaa tgactgcagc attacatgca cttgacaaaa
     3061 cggacagaaa cggctatgtt ttcacctctg aatctgtctc ggaaggtcat ccggataaag
     3121 tttgcgatca gatatcggat gctatattag atgcttattt agcagctgat cctcattcga
     3181 aggttgcctg tgaaacatta gctacggaaa acagaataat cctatcagga gaaatttctt
     3241 caaaagcgac agtagatatt gaatctattg tccgtaaaac aattcaagaa attggctata
     3301 cctcccccaa caaaggtttt gattataatt gccaaataaa caattactta caccgtcaag
     3361 aaagcgccct ggaagaaaat accggagctg gagatcaagg tttaatgttt gggtatgctt
     3421 gttcccaaac tcaaaatatg atgcctttgc caattgaatt agcacataag ttactgttca
     3481 atcttgctac ggcacgcaaa aaaggaacaa taaaacatct gctccccgat tctaaaagtc
     3541 aggtttctat gcgttattta ggaagaaaag ctgtagattt gaaatgcttg gttatttcta
     3601 cccatcatga tattttaact gaaatggaat ttgaggagct aaaggaacag atacagcagc
     3661 tgattataat tcccagcata agagatatcg aagatggctg caagggttat tttgaaacta
     3721 agaattgtga aataaaaatt aatcccttag gtccttggca ggatggaggc cccgcttttg
     3781 atactggcct tacaggaaga aaaataattg tggatactta tggaggttgg gctcaacatg
     3841 gaggaggagc tttttctgga aaggacgcca ctaaagttga tagaagcgct gcctatatgg
     3901 gccgtcatat tgctaaaagt attgtagcca gcggtttagc tgatgagtgt ttaattcaat
     3961 ttagctttgt gattggtgaa cagaaaccaa tatcattgat ggtagatact tttggcagtg
     4021 gcaaagaaag cgatgcagaa attgaaagga ttatcgcaaa aaactttgac ttgacagtta
     4081 gcgggatcat agattattta aatttgcgta aacccatata ttttcctact gcctcttacg
     4141 gtcattttgg cagagataat ttttcctggg aaagagtaaa aaaaattacc agatgatgga
     4201 gaaaacagca tagcctagct gattgcatac tgatgagtaa actatatact gaccttgcaa
     4261 atagccaaaa cttctttcta attgcgggac cctgcgttat tgaagaagaa agaataatgt
     4321 attccattgc agaaacacta aaagagcttt gtactcgaaa agggataccg ctgattttta
     4381 aagcatccta taaaaaagca aatcgcagtt ctgtagattc ttttagtggt cctggtttag
     4441 aggaagggct acaaaccctg gcaaagatca aacaggattt tgagcttcca gtgctaactg
     4501 atgtccatga atgtatagaa attacttctg ctgctgatgt ttgtgatatt ttacagattc
     4561 ctgcttttct atcccggcag acggatttga taaaaaatgc tgccgccact ggaaagataa
     4621 taaatatcaa aaaagggcaa tttatggctc cagaagatat gcaatttgct gccgaaaaag
     4681 caagaagcac aggtaatgag caaattttat taactgagcg cggaaccact tttggctacc
     4741 ataatttagt tgtagatttt cgcagtttta ctattatgag tcaatttggc tatccggttg
     4801 tttatgatgt aactcattct ctgcaaaggc cttcgctaag caatatttct ggtggcaatc
     4861 cagaatatgc tttttcaatg gctaaagccg caattgccac aggaaaagta aaagggcttt
     4921 ttatagagac tcatccagaa ccccaaaaag ccaaaagcga tgctgctagt atgctttctc
     4981 tggataaaat ggaatccctc ctggatggct gtttacagtt attaaactga aggatcacag
     5041 atatgtacga taaaagagca ttaaatagac ccaataaaag agtagttccc tggaatgaaa
     5101 ttaaactgct tgtttttgat tgtgatggag ttttaacaga cggacgcttc gtttacggaa
     5161 ctaataatct ggaaatcaag aattttcacg gacacgatgg tttgggtttc aaacttttac
     5221 aacatacgga tataatagta tgttttataa caggacgcag ttccgaggca ttggaaagac
     5281 gctgtctcga tttaaatatc acatatcttt ttcagaatgt tgccaacaaa aaagagtgct
     5341 tggataatct attaaaagac ttaagcttaa cttatactaa tgtagcttat atgggtgacg
     5401 actggaatga tgttccggta atgcgaaaag tagcattttc tgcagcaccg gctgatgctc
     5461 aacctggaat tattcatctt gctgattggg tttccgaatt taaaggtggt cacggtgctg
     5521 caagagatct gataaactat gtccttattc ataaaggcat ctatgaaaaa acgattctgt
     5581 cctatttgga tagcatcggt tagtgttgtt attctgcttt cttcctgtca aaaagtagat
     5641 ttacaatctc atacgggtgt tttagcacgt gatttaccgg atgaaattag ttataaagtt
     5701 cagctaaccc agctcaataa cgaccacaat gagtacattt tagaagctga aaggattgag
     5761 aggttttatg ataggcgttt actttatgct tataaagtaa ccttgaccac ttttgataaa
     5821 aacaatcaga taagctctgt tattaaagcg gatacaacta ttgtagatga tgctcgtaat
     5881 ctaatttttg ccaatggaaa tgcttctcta aattcaccta atggaacttt atctactcct
     5941 agaatggctt gggatagaac tgttgatgaa ataactgccc ctttagaagt aactgttact
     6001 cgtaaaggaa atgtgatgta tggtagaaat ttaagaacta acagcaagct tagttttgta
     6061 gagatggatg ctgtgtctgc agaaggatat tttaaggaag aggaaataaa ctggtgaaaa
     6121 ggattttgtc ttgtctgata ctgtttttga tgcttttttc tttatatgca aaagggcagg
     6181 ctgatgaaaa atttcgctta attcattccg ataaactgtt tatgactaag gcaggagagg
     6241 aacaggcact tgaattgaac ggtaatgttc atttctttta tggtaatacc gaattcttat
     6301 gtagaaatgc cctgatttta gatactcaaa aaatagctcg tctctctgga aatgtaattg
     6361 ttaagaatga cagtttaacc ttggaagcag atactttagc ttattatcga attccggatt
     6421 tgatgaattt gggtggtaga atcactgcta ctgaaaagac aaaaacagga atctatcgct
     6481 ggataaaaag caattttgcc tcttacgata aagcaaacga tattttgacc acttggaaca
     6541 gagtaaacag ttttgattat caagaaaacg ctcatatcca atgtggatat gcccaatggg
     6601 atagaaaaaa gggttatgcc atcttactgg aagagccatt tataacttca ggaacaaaag
     6661 ataccctttc tgtagctgcc gaaaaaatgg agtattttga tcaggaacat aaactgattg
     6721 ctacctttaa tgtaaaagta gagagaaatg aattccatgc taccagcgat tttctcatct
     6781 atttcctgaa tgaagaaaaa gcaattttta ctggtgaacc aaagttcttt tccaattttg
     6841 cggatgctaa agctatggaa tttcatctgt ttttaaaaga ccgctcccta atgcgagcag
     6901 aattaataga ttcctgccat atcgattttg ccgaagaaca atttatggaa aagaaaaata
     6961 aggtagatgc agacttagtt actatggatt ttttagatga ccaactccgt atttttgtgg
     7021 ccgaagggaa tgtagattat tattttcaac aggatcaaac agaaaagaag gactttatga
     7081 tcaatagtgc tactggagca tatctgcaag ccaactttga tgataacagc aaactgcaat
     7141 ctctgcaaat gaaaactgat attaagggga aatatgtgtt tcaacagtaa ggtcgcttaa
     7201 tagaaagaac ctaagccttt ttgccgtata tttctgaaaa acccacttta ttatttcttc
     7261 atttctccaa gttttatttg ctctttctga aaaaagcaat atcttttaaa tagagttaaa
     7321 ttttttttat gtgagaagga gttagatatg cagataccat ttaatatgaa tcggttgaaa
     7381 gatacggaac ttcgctaccc gcagcgtttt gccaatatgt taaccaagaa ttatggatta
     7441 atttttttca atgaaggcaa caaggcatct caagatagca atcatgctat aattcaagat
     7501 ctgattggag tggaaagttc tctgcgtgat atagaatcct tttacaaaag caaaggtata
     7561 cgcccctgtt tatatccctc tcttacgcaa aaagaattag ctgttttagc accgctaatc
     7621 gagaaccata atttcaaact ggttatgaaa aacagtgaat ttttcctgca cgaccacgaa
     7681 agcagcttac gagaaatcga cgatctgaaa attgaaagag tccataagct agatattgat
     7741 attatggaaa ctatcgctat tgaatatgga ggcgattgga caatacgagt ggtggaacgt
     7801 catttactgc atccttccta tcatttttta ggcggctatt ataaaggtga attagctact
     7861 atggcttcca ttagtatttt cgccggttac acccgaatcg atgatgtttt taccagggat
     7921 aaattcagag ggaaaggttt cgccggagcg atgattcacc atctggtaaa ttatcacagt
     7981 agaatctcgc caaactatct ttatgtgcag tccgatttgc cctcaactat taaaatttat
     8041 gaaaaagctg ggtttagctg tctgcctaga gatttcgatt gctggatcgc acataaggaa
     8101 aataactgat ttatttacct gaagaatttt gcacatagtg cttagtcgcc taaatattac
     8161 ccttttgtcc tatactgaac ctacaaaatt cattattgct taattcaatg ccttttatcc
     8221 ctgtgttttg ttttttaatt tagctatcta ccggtgggta attttagtgc tactttatat
     8281 tctacgatag aaaagaggat aaatattaaa tcagctataa attacttgac agctttagca
     8341 atgctaataa ttgtggatat agatgttaga tatgctacca acttagtaac tctaacatca
     8401 taaggagtgt gatatgattg gacaaagaat caaacaaatt cgcactgctt taggaattaa
     8461 gcaggttgaa ttagccaagg ttctaaaaat gaatccatca gctgtgtctc aaatagaaag
     8521 cggacgcact aatccctctc tggaaactct ttctgaattg ggtaagatat ataatatcaa
     8581 tcttcattgg ctgattacag gaacaggaga aatgttcaat acaatagcag aaactgaatc
     8641 tcaatccggt gcctgggata attttcaaaa gctgatgaat gaacggctgg aagaaattgt
     8701 ccaagcccgc aaggatttaa tggattcgga tatcgttgag atagcggtta gtggagaaat
     8761 cgcagctggt gcacccatag aaaattatgg agcttcactt gaagttgtaa cagttagtcg
     8821 ttcattgata aacggttctt taagtgattt tgttgctctc cgagttaatg gaagaagtat
     8881 ggaaccagat atcagaaatc aagatgtagt gttaattcgt cttagtaatg aatggcgcga
     8941 gctggaaggt aaaatttgtg caattcgcat agacggtggc ataaccttga agcgattatt
     9001 acttgatgat gcccgaaaaa tggttgtttt agttccgatc aatgaagatt ttcaaccgat
     9061 tatagttgat ccagatagcc atagtgatgt aacattaatt ggatcgctat attttctttt
     9121 cagaatatta agttaaagtg aggtataaag cataatgtct tctatctatc ttcttctttt
     9181 agcacttatt ttgctatctt tattgaacct ctatattttg ttatttcacc ggcgcaaaga
     9241 aagcagaaca gataatttaa atgaactgca aaatgctttt aatagttttg aacattataa
     9301 ccgtgaagaa atcagcaaat tacgcacaga gcttttaaat atcagcgcag aaaaccgtaa
     9361 agaactaaat gaatctatcc gagcccaaaa tgaagtgctt attcaacaaa gcactaccag
     9421 ccgagaagaa ttgaataatt cctttaatgc cttaaaacaa cagtttaata atgacgcagc
     9481 cacaaatagg gaagaactga aaaaagcact gaacgacctc tccgataatt tttccagaaa
     9541 aatgacagaa ctgataaata tccaacaaca ggaagcagaa aactccagaa aaactttaga
     9601 atccaaaatg gaacagatcc gtcaaagtaa tgagaccaaa cttgaacaga tgcgtcaaac
     9661 agttgatgaa aaactccacg atactttaga aaagcgtttg ggggaaagtt ttaagcaagt
     9721 tagtgaacgc ctcgagctgg tacataaaag tatgggagaa atgcaaaatt tggcaaacgg
     9781 agtaggcgat cttaaaaaag cacttactaa tgtaaaaaca cgtggtgttt taggagaaat
     9841 tcaactggag aatttgctgg aggaaatgct tactccagag cagtacgaaa agaattttag
     9901 gcccaataaa aatagggatg aaaaggttga atttgccatt cgtttacccg gaaaatcaga
     9961 tgaacaggat tttgtgtatc tgcccattga tgcaaagttt cctattgagg actactatag
    10021 aattttggat gcatacgatt tgggagattt agcctctgta gaaaatgccc aaaaaagttt
    10081 ggtgcaaaaa attaagaact gcgccaagga tataagagat aagtatataa atcctcctat
    10141 aacaacagat tttggaatgc tatttttacc ctttgaaggt ctgtatgctg aagttttaag
    10201 aaatactgga ctttttgaaa ccattatgcg tgattatcag gttattattt gtggtccaac
    10261 tactacagcc gctgttatta atagtttaca agtaggtttt cgaactctgg ctattcagaa
    10321 aa
//
LOCUS       DAOSTH010000058         9929 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_6591_length_9929_cov_15.760178, whole genome shotgun sequence.
ACCESSION   DAOSTH010000058 DAOSTH010000000
VERSION     DAOSTH010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9929
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6591_length_9929_cov_15.760178"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1138)
                     /gene="dxr"
                     /locus_tag="PLB65_06560"
     CDS             complement(<1..1138)
                     /gene="dxr"
                     /locus_tag="PLB65_06560"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOQ78226.1"
                     /translation="MVNKSLALLGATGSIGTSTFAVAAEQNIPIVLASADKNHLLLLK
                     YAKQHNIPSLFFTGIEDPTLQTKLKAENPEHKIYFGETELIKALANENYDIALNAIAG
                     SAGLRYSFAILKRNKYLALANKESLVMGGHLLTKMLSDKPIIPVDSELSALFQAIGKH
                     PANEIQHLHLTASGGIFRDLPLEEFDKITPQQALKNPNWTMGAKVTLDSATMFNKALE
                     VMETHWLFNQPYSKIKAVIHPQSLIHSLVEFIDGSFLAQISNPDMKLPILYALSWPQR
                     VHSQLVQTDLTSLAPLTFREIDPKRYPMFYLGLEVANAGGIYPTVINSAVEAASFLFL
                     QNKIPYLKMFDIVKRALDDQEPISEPDLETIMEINYQTYKKVLQQ"
     gene            complement(1132..2076)
                     /locus_tag="PLB65_06565"
     CDS             complement(1132..2076)
                     /locus_tag="PLB65_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78227.1"
                     /translation="MKIQVLVICLLLITLPIAATKYAGEIFAISPGVLSSAMGSTGLT
                     YDSSFSVGWWNPALLGLISDNGIELMRSEHFEGLMEQNQMSVILGSTTKTSLTLNNLC
                     IDNVKLTKLENPDDSLSSDNRPYVWKTISNQDFIFYAGLARQVSNSLYLGISPKLAYR
                     SLAENSGYAFGADIGMLWQMNKRARLGLNLRDFFSTQVLWEGGENETVIPNLDLELGY
                     AFSVKAKIPVHLAVRSEILLENREATVETGDFSADFHAGLAVQPIPNLRLLAGYDVDC
                     FTTGIGVSYKSFGLNYAFRNGSEDDLGYSQRISATYQW"
     gene            complement(2142..2217)
                     /locus_tag="PLB65_06570"
     tRNA            complement(2142..2217)
                     /locus_tag="PLB65_06570"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2182..2184),aa:Ile,seq:cat)
     gene            2464..3285
                     /gene="nadC"
                     /locus_tag="PLB65_06575"
     CDS             2464..3285
                     /gene="nadC"
                     /locus_tag="PLB65_06575"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOQ78228.1"
                     /translation="MFLDEFIRKSLEEDIGTGDITTRYLDFEPQISVAYIISKATGVI
                     AGIDIAKQVFKMVDPELKITLYSKDGDSVKPNEEIMRLEGRPSSILQGERTALNFMQR
                     LSGIATKTAKYVSLLEGLNVKLLDTRKTTPLLRSLEKYAVRVGGGYNHRFGLFDMVLI
                     KENHIRACGSITEAVNRIRKHNISYKIEVEVTNAKEVAEAVNAGVDRVMLDNMTPEQI
                     RKVVKKYKDILEFEVSGGITEENIRTYAETGVQFISSGALTHSYKSLDISLLFKE"
     gene            3289..4020
                     /locus_tag="PLB65_06580"
     CDS             3289..4020
                     /locus_tag="PLB65_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HOQ78229.1"
                     /translation="MEEELKTLAKMQALDDQIGRYRILQKELPKQLNEIIESVEEATA
                     NLLAVETERAEINKKQRSVEMEIKQMQEQSKKYSAQLTEIKNNKEYKALNSEIAYIKD
                     KISDNESSLLELMELENEIKEKIATAKSVLEKAEALKKEKESDLREQIASLDTKIEEV
                     RTQRNDLARTLPVSLVKYYGNMIKHKQNQAVAYTREGSCGACGFVIRQQMRIELQLRR
                     KIIYCENCGRILMNRFEDKTDDASS"
     gene            4078..4437
                     /gene="rnpB"
                     /locus_tag="PLB65_06585"
     ncRNA           4078..4437
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLB65_06585"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4576..5427
                     /locus_tag="PLB65_06590"
     CDS             4576..5427
                     /locus_tag="PLB65_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78230.1"
                     /translation="MLKKLVFVLILVTAIALFALVSHPPKEYVLQTDLLKEASGIDLG
                     IVNKQTLWSHNDSGNQAEIYALGTDGKLLATLTINNTKNRDWEDIAIAKDPHTGKSYI
                     YIADIGDNNAKYNSVYIYRVREPLIANNDKIIQSSQVDTIEIQYEDGPRDAEAIFLEP
                     KTGDIYLISKREEQVGLYRIAYPFNFQNISTAERICSLPLSWVTAADLSDNGKLLIIK
                     TYTSIYRYKTKRDDKNIITLSQNFKSLPYITEPQGEAICWDAKNKGYFTLSESATSVK
                     QALYHYK"
     gene            5766..5966
                     /locus_tag="PLB65_06595"
     CDS             5766..5966
                     /locus_tag="PLB65_06595"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HOQ78231.1"
                     /translation="MLEIGYTSQFERDYKKYRNIQNLKVVLKLMSGKPLPARYKDYQL
                     KSGKSECRECYLTPNILVFYRI"
     gene            6063..7235
                     /gene="ygeW"
                     /locus_tag="PLB65_06600"
     CDS             6063..7235
                     /gene="ygeW"
                     /locus_tag="PLB65_06600"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HOQ78232.1"
                     /translation="MDNRERASALIEELETLQPDLYGKDFLLTWENCFDNLKAVMLTA
                     EILQCLHKEKKPWRIFDYGLAISIFRDNSTRTRFSFASAVNGLGLALAELDEVKSQIA
                     HGETVRETANMISFLTEVIGIRDDMYPGEGHSYMLEVAQAVSDGYIQGVLAQRPTIVN
                     LQCDLDHPTQVLSDLLKLKDYFGGWENLKGKKLAMSWAYSPSYGKPLSVPQGVINLMT
                     RFGMNVVLAHPEGYNLLPETLKSAAFFAQESGGSFAQTHYMEEAFKDADVVYPKSWAP
                     LAVMQERTELLRIGNKQGLQELEQLCLANNERFIDWECDEEMMKLTKNGEALYEHCLP
                     ADISDVSCERGEVSQSVFERYRYHTYQEAGYKPFVIAAMILNSKVQNPVAKLKSFL"
     gene            7317..8762
                     /locus_tag="PLB65_06605"
     CDS             7317..8762
                     /locus_tag="PLB65_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOQ78233.1"
                     /translation="MPKLITKIDPVVAKKNAKRCKERGIILPTFHQQMFPDTIPETIK
                     EKLKPIGLWDINPLNLFRINWKNDINTGLFGKPNYLEIPSELTGVKARIIGLAGKYFP
                     TGTHKVGAAYACLVSRLVSGTFDPEIHKAVWPSTGSYCRGGAFDCALLACTAVAILPE
                     EMSKERFDWLREIGAEIYVTPGCESNVKEIYDKCAELRKDPTFVILNHFDEFGNPLWH
                     YHITGNAISEVFEAIKKPGDRLSGYVSATGSAGTIAAGDFLKKQYPSCKINATEALEC
                     PTLLMNGFGKHRIEGIGDKHIPWIHNVRNTDLVTAIRDEDCMRLLRLFNEPIGIKYLK
                     QNGINPNLADKLDLLGISSICNLLSAIKMAKYYEFNEQDIIFTSFTDSSLMYHSRLQE
                     QTAAKGEYSELQAALDREGVLNAQSYDNLLELSYKEKKRIHNLKYYTWVEQQGKSETE
                     LIQQWEPEYWIETFENNLSELDNAIEEFNNS"
     gene            8780..9304
                     /locus_tag="PLB65_06610"
     CDS             8780..9304
                     /locus_tag="PLB65_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HOQ78234.1"
                     /translation="MKVTAIILASGKGKRIGLPKADISLMGISFIEQIRKILTAAGVE
                     DIFVARYENTPDMLATLRRAVSELKSKQENSGYLVFPVDFPFVFADTVQKLIKTHYQN
                     PLAVLRPIFNQQRGHPILIPSELNLDKEDKNQGLQYIIHTCSLPVVDIPVKDSGILKN
                     VNTKEDLELWMPKK"
     gene            9286..9834
                     /locus_tag="PLB65_06615"
     CDS             9286..9834
                     /locus_tag="PLB65_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOQ78235.1"
                     /translation="MDAKEMIWERHSVRDFLPTPIPAEIIQEILEAGHLAPSTQNRQP
                     WRYLVFTENAKIQKMALHCGLIGLSNFFIRQAPCLIIACADLKDNIKINGKDYYLVDT
                     AISFHQMLLYARSYGIESCWLAAFSEKKLKAYLKIPENWKIVAMAPLGYPKEKKGLYS
                     KAVSNFANSKDRKPLESVVRFM"
BASE COUNT         3260 a         1745 c         2069 g         2855 t
ORIGIN      
        1 tttgttgcag aactttttta taggtctggt aattaatctc cataatggtt tccagatcgg
       61 gttcagaaat aggttcttga tcatccaatg ctctttttac aatatcgaac atctttaaat
      121 aaggtatctt attttgcaga aagagaaaag atgccgcttc cacagcagaa ttgataacgg
      181 tgggataaat tcctccagca ttggctacct ccaaccctaa ataaaacata ggatagcgtt
      241 tcggatcaat ttctctaaaa gttaaaggcg caagagaggt taaatccgtt tgcacaagtt
      301 gcgaatgaac tctttggggc caggaaaggg catataaaat cggcaatttc atatctggat
      361 tgcttatctg agctaaaaaa gagccatcaa taaattccac cagagaatgg ataagggatt
      421 gaggatgaat aaccgctttt attttagaat agggctgatt aaacagccaa tgagtttcca
      481 taacttccaa tgccttattg aacatagttg ccgaatccag agtaactttg gctcccattg
      541 tccagtttgg atttttaagt gcttgctgag gtgtgatctt atcaaattcc tccaaaggca
      601 ggtcgcgaaa aatacctccg gaggcagtaa gatgcaaatg ctgaatttcg tttgcgggat
      661 gttttccgat tgcttgaaaa agagcgctaa gttcgctatc cacaggtata attggcttgt
      721 cggaaagcat tttggttaag aggtgacctc ccataacgag ggattcttta tttgccaaag
      781 ccagatattt attgcgtttt aagatggcaa aactataacg caatcctgcc gaaccggcta
      841 tggcatttaa agcaatgtca taattttcat tagccagtgc tttaataagc tcggtttcgc
      901 caaagtatat tttgtgttca ggattttcag ctttaagctt agtttgcaat gtaggatctt
      961 caattccggt aaaaaaaagc gaggggatat tgtgttgttt tgcatatttt agcagtaata
     1021 agtgattttt atctgccgaa gccaaaacga taggtatatt ttgttcagca gccacagcaa
     1081 aagtggaagt tccgattgaa ccggttgccc ctaaaagagc taatgactta tttaccattg
     1141 atatgtagcg gaaattcttt gcgaatagcc caaatcatct tccgaaccat tacggaaggc
     1201 ataatttaag ccaaaacttt tatatgatac tccaattccg gtagtgaaac aatctacatc
     1261 atatccagca agtaaacgca aattgggaat tggttgaact gctaggccag catgaaaatc
     1321 agcactaaaa tctccagttt ccactgttgc ttctctattt tccaataaaa tttcgctacg
     1381 cacagcaaga tggacaggaa ttttagcttt tacagaaaaa gcatatccca attccagatc
     1441 cagattggga atcactgttt cattctcgcc tccctcccaa agaacttgag tgctgaaaaa
     1501 atccctgaga tttaatccta aacgcgctcg tttattcatc tgccaaagca ttcctatatc
     1561 cgctccaaaa gcataaccgc tattttcagc caaagacctg taagccagct tgggtgaaat
     1621 tcccaaatat aaactgttac taacctgcct ggctaaaccc gcataaaaaa tgaaatcctg
     1681 attgctaatt gttttccaaa cataagggcg attgtcgcta cttaagctgt cgtccggatt
     1741 ttccagcttg gtaagtttta cattatcgat gcataagtta ttgagagtga gagaggtttt
     1801 cgtagtagag ccaagaatta cactcatttg attttgctcc atcaaaccct caaaatgctc
     1861 actacgcata agttctattc cgttatcaga aattaaacct aaaagagctg gattccacca
     1921 accaactgaa aagctggaat cataagttag ccccgtagaa cccatagctg aagaaagaac
     1981 acctggactg atagcgaaaa tctctcctgc atattttgtg gcagcaattg gtaatgttat
     2041 caatagcaag cagataacga gaacttgaat tttcatttag ttcctctctt ctgttttccc
     2101 tgttgaacag gaatttataa tgtaaaaaag ggggagaaaa atggtgggcc cagagggact
     2161 cgaacccccg accaaccggt tatgagccgg tagctctacc aactgagcta taggccccct
     2221 aaatttattc gacttaaaca caaaattcat aagcccgaat ttcgtcaagc caaaaaatgt
     2281 gaatgcttat gatattttgt cttgacagat acagcgcaag caaaatatgg gtgattagta
     2341 taaaggaaga tagatatatg tgttaccaag acaagaaatt atgcgagctt aacaaaaacg
     2401 aaaatataat aacaggaaag catttgcaaa acgcaaatgc ttttttttta tctcggagga
     2461 agaatgtttc tcgatgaatt cattcgtaag agcttggaag aagatatcgg tacaggtgat
     2521 ataacgacca gatatcttga ttttgaaccc caaattagtg ttgcttatat catctccaaa
     2581 gctacaggtg ttatagctgg tattgatata gcgaaacaag tatttaagat ggtcgatcct
     2641 gaactgaaaa taactttata cagtaaggat ggagattcag taaaacctaa cgaagaaatt
     2701 atgcgtttgg aaggacgccc ttcctctatt ctacaagggg aaaggacagc tttaaatttt
     2761 atgcaacgtc tttcggggat tgctactaag accgctaaat atgtttctct tttagaagga
     2821 ttgaatgtta aactactgga tactcgtaaa acaacaccac tacttcgttc gctggaaaag
     2881 tatgcagtac gagttggagg cggttataat catcgttttg ggttatttga tatggttcta
     2941 attaaagaga atcatattcg tgcctgcggt tcaataactg aagccgtaaa ccgtattcgt
     3001 aagcataata tcagctataa aattgaggtt gaggttacca atgcgaagga agttgcagaa
     3061 gccgtaaatg ctggtgtaga tagagttatg ctggataata tgactcctga acagatcagg
     3121 aaagtagtaa agaaatataa agatattttg gaatttgaag tatcgggtgg aattacagaa
     3181 gaaaatattc gtacttatgc cgagaccgga gttcaattta tttcctctgg agctctcact
     3241 cattcatata agtccctgga tattagtttg ttatttaagg agtaagcgat ggaagaagaa
     3301 ttaaagacat tagccaaaat gcaagccctg gatgatcaga ttggccgtta tcgcattttg
     3361 caaaaggaac tacctaaaca gcttaacgag attatagaaa gtgtagaaga ggcaactgcc
     3421 aatcttcttg ccgtagaaac cgaacgcgct gaaataaata aaaagcagcg ttctgttgaa
     3481 atggagatta aacaaatgca ggaacaaagc aaaaaatatt ctgcacagct tacagagatt
     3541 aaaaacaaca aggaatataa ggcattaaac agtgaaatcg cttatattaa ggataaaatc
     3601 tctgataatg aatcctctct tttagaactg atggaattag aaaacgaaat caaggaaaaa
     3661 atagccactg ccaaatctgt tctggaaaaa gcagaagctt tgaagaaaga aaaagaaagt
     3721 gatttgagag aacaaattgc ttccctggat actaaaattg aggaagtccg cactcaaaga
     3781 aatgatttag cacgcacttt accagtttca cttgtaaaat actatggtaa tatgataaaa
     3841 cacaaacaaa atcaggcagt agcttatacc cgtgaaggtt cctgtggagc ctgcggtttt
     3901 gtgattcgtc aacaaatgcg gattgaactt caattacgaa ggaaaattat ctactgcgaa
     3961 aattgcggac gcattctaat gaaccgcttt gaagataaaa ctgatgatgc ttccagctaa
     4021 tctaacccga atgaatatgc agtttgcttt atcatagaat tgcattatat tatgtatggg
     4081 gaagattaga tgttcgctgt aaaaatgcag aggaaagtcc ggacaccata gggcaggata
     4141 cttcctaaca ggaagctgta gtgatgcaga gatagctcca cagaaagtaa accgctattc
     4201 agttttttct gaagcagcaa gggtgaaacg gcggtgtaag agaccaccgg tatctttggt
     4261 aacagagata gccaggaaag ccttatctgg tgcaatgcca aataggaaag caatgatgtt
     4321 gcccgcatcg ctttcgggta ggcagctgga actgttttgt gagaagcagt caagatgaat
     4381 gaacatcact ttcaggaaac tgaaagcaca gaatccggct tataatcttc cccataatta
     4441 tatttggaac gacagtgttt cattttataa cttgcgcctg gaatcaaaag ttaacgataa
     4501 atttagaaca gctgataagt ttattagctg ggagtggaca gggatttcaa tcatccagaa
     4561 atatatgagg tattaatgct gaaaaagctt gttttcgttt tgattttggt tacagccatt
     4621 gccttatttg ctttggtcag ccatcccccc aaggaatatg ttttacaaac tgaccttcta
     4681 aaagaagcgt ctggtattga tttgggcatt gtaaacaaac agacgctttg gagccataac
     4741 gattctggta atcaagcaga aatttatgct ctgggaacag atggcaaatt actggctact
     4801 ttgacaataa acaacactaa aaaccgcgat tgggaagata ttgctattgc caaagacccg
     4861 catactggga aaagctatat ttatattgct gatattggtg ataataacgc taaatacaat
     4921 tctgtgtata tttacagggt tcgagaaccc ttaattgcta ataacgataa aattattcaa
     4981 agcagccagg ttgatactat agaaatacag tatgaagacg gacctcgcga tgcagaagct
     5041 attttcttgg aacctaaaac tggagatatt tatttaatta gcaaaagaga agagcaggtg
     5101 ggtctatatc ggattgccta tcctttcaat tttcagaaca taagcactgc tgagagaatt
     5161 tgttctttgc ctttatcttg ggttactgca gctgatctga gtgataatgg aaaactacta
     5221 ataattaaga cttatacaag tatatatagg tataaaacca aacgagacga taaaaacatc
     5281 attaccctct cccaaaattt taaatctctg ccttatataa ctgagcctca gggtgaagct
     5341 atctgctggg atgcgaaaaa taaaggctat tttactcttt ccgaatccgc tacaagcgtg
     5401 aaacaggcat tatatcatta taaatgatac ggaactaaga aaaaaagagt aagatagtga
     5461 aagttgagaa ggatagatta ggaagatgga aatttaggaa agtgtgatag ctttgttcac
     5521 agtgcacgag ttcaaagttt ttgttttgga ggtatcggta actataataa atctactgtc
     5581 tctgaatgga ataaccgttc attcgcttct tcaaaatttg agcttgacat aaacaaagaa
     5641 gtgaaacaaa tgaaatacat taactcttat caaaaataag gaaataaata tggaaccaaa
     5701 acatccggaa aaactatcgg tttatagtaa tattgatggc ttcctgaatt ctttagagag
     5761 tcgatttgct cgaaatcggt tatacttctc aatttgagag agattacaag aaatatcgga
     5821 atatccaaaa ccttaaagtg gtactaaaat taatgtcggg aaagccatta cccgccagat
     5881 ataaagatta tcagctaaaa agtggaaaaa gcgaatgccg agaatgctat ttaactccta
     5941 atattcttgt gttctataga atataagatg agttcttaat cctgattagg attggctcgc
     6001 attccaattt gtttgattta atattttctt agccctttct gaaaagggaa gaagaggtga
     6061 atatggataa tcgagaaaga gcaagcgctc tgattgaaga gctggaaacc ctacaaccgg
     6121 acctgtatgg caaggatttt ttattaacct gggagaactg ttttgataat ctaaaagcag
     6181 tgatgttgac ggcagaaata ctgcaatgtc tgcataagga aaagaaaccc tggcgtattt
     6241 ttgattacgg tttggcaatt tccattttca gagataatag cacgcgcact cgtttcagtt
     6301 ttgcttctgc tgtaaatggt ttaggacttg ccctggcaga attggatgaa gtgaaaagtc
     6361 agatagcaca cggagaaact gttcgcgaaa cagcaaatat gatatctttc ttaacggaag
     6421 taattggtat ccgtgatgat atgtatcccg gagagggaca cagttatatg ctggaagtgg
     6481 cgcaggcagt ttccgatggc tacattcaag gtgttttagc ccaaagacca actatagtga
     6541 atttacagtg tgacttagat catcctactc aggtgctttc cgacttactg aaactaaagg
     6601 attattttgg tggctgggaa aacttgaaag gcaagaaact ggctatgagc tgggcatatt
     6661 ctccatctta tggaaaaccg ctttccgtgc ctcagggagt aataaatctg atgactcgct
     6721 tcggaatgaa tgtagtttta gcgcatcctg agggttataa tttgctgccg gaaaccttaa
     6781 aaagtgctgc cttttttgcg caggaaagtg gaggttcttt tgctcagaca cactatatgg
     6841 aagaggcctt taaggatgct gatgttgttt atcccaaaag ctgggctcct ttggctgtta
     6901 tgcaggaaag aactgaacta cttagaatag gaaataaaca aggtctgcag gaactggaac
     6961 agctttgttt ggccaataat gaaaggttta ttgactggga atgtgacgaa gaaatgatga
     7021 aacttactaa aaatggagaa gctctttacg aacactgtct acctgcagat atcagtgatg
     7081 taagttgtga acggggagag gtttcccaaa gcgtatttga aagataccgc tatcatactt
     7141 atcaagaagc gggttacaaa cccttcgtga ttgccgcgat gatattgaac agtaaagtgc
     7201 aaaaccctgt tgccaagttg aagagttttt tgtaaattca atatttcttt acaaaaaagc
     7261 ccagttcctg ttcttagtta atttattatt tgcatattta tatataggag gtataaatgc
     7321 caaagctgat tactaaaata gaccccgttg ttgctaaaaa gaatgccaag cgttgtaaag
     7381 aacggggtat aattctgcct accttccacc aacagatgtt tccggatacc attcctgaaa
     7441 caattaaaga aaaattaaag cctatcggac tttgggacat taatccctta aacctattta
     7501 ggataaactg gaagaatgat ataaatacgg gacttttcgg caagcctaac tacttggaaa
     7561 ttccttctga actaacaggt gtaaaagcaa gaataatagg tttggcggga aaatattttc
     7621 ctacaggaac gcataaagtt ggagctgctt atgcttgttt agtttcgcgt ttagtatcgg
     7681 gtacttttga tcctgaaatt cataaagcag tttggccatc cacaggtagt tactgtagag
     7741 gcggagcttt tgattgtgct ctattggcct gtacagcagt agctattcta ccggaagaaa
     7801 tgagtaaaga gcgatttgac tggctacgcg aaattggagc tgaaatatat gtaactcccg
     7861 gttgcgaatc caatgttaaa gagatttatg ataaatgtgc cgagttgcgc aaagatccta
     7921 cttttgttat tttgaaccat tttgatgagt ttggcaatcc tctctggcac tatcatatta
     7981 ccggtaatgc tatcagcgag gtctttgaag caattaaaaa accgggtgat cggttaagtg
     8041 gatatgttag cgctacagga agtgctggaa ctatagctgc tggtgatttt ttaaaaaaac
     8101 agtatccttc ttgtaagata aatgccaccg aagctttgga atgcccaact ctgctaatga
     8161 acggttttgg taaacatcgc attgaaggaa ttggcgataa acatatacct tggattcata
     8221 atgtgcgtaa tacagatctg gttacagcta tcagggatga agattgtatg cggcttttac
     8281 gcctattcaa tgagccgatt ggaataaaat atctgaagca aaacgggatt aatcccaatt
     8341 tggctgataa acttgatctt ttaggaattt cttcaatctg taatctccta tccgctatta
     8401 agatggctaa atattatgag ttcaacgaac aggatattat ctttaccagc tttacagaca
     8461 gttctttaat gtatcattcc cgtctgcaag aacaaactgc cgcaaagggt gaatattcag
     8521 aactgcaggc tgctttagat agagaaggag tcctaaatgc tcagagttac gataacctgc
     8581 tggaattaag ttataaagag aaaaagcgga ttcataacct taagtactat acctgggtgg
     8641 agcaacaagg taaatccgaa acagaactaa tccagcaatg ggaaccggaa tattggattg
     8701 agacctttga aaacaatcta tctgaactgg ataacgcaat tgaggaattc aataatagct
     8761 gagggctgag agcagaggga tgaaagttac tgcaattatt cttgcttccg gaaagggaaa
     8821 gagaataggt ttacccaaag cagatatcag cctaatgggg ataagcttta tagaacaaat
     8881 aaggaaaatt ctaaccgctg ccggggtgga agatattttt gtagcaaggt atgaaaatac
     8941 tcctgatatg ctggctactt tacgaagggc ggttagcgaa ctgaaaagta aacaggaaaa
     9001 tagcggttat ctggttttcc cggttgattt tccttttgtt tttgcagata cagtccaaaa
     9061 attgataaaa acgcattatc aaaatccttt ggctgttctc cgccccattt ttaatcagca
     9121 aagaggtcac ccgattttaa tcccttcaga gcttaatctg gataaagaag ataagaacca
     9181 aggattacaa tatatcattc atacctgtag tttgcctgtt gtagatatcc ccgtaaagga
     9241 ttccggaata ttaaaaaatg ttaatactaa agaggatttg gaactatgga tgccaaagaa
     9301 atgatttggg aaagacacag tgtgcgagat tttttaccta ctcccattcc tgcagaaata
     9361 atccaagaaa tattggaagc ggggcatttg gctccttcca cgcaaaatcg tcagccctgg
     9421 cgatatctgg tttttacgga aaatgccaaa attcaaaaaa tggctttaca ctgtggattg
     9481 atcggattat ctaatttttt tatccgacaa gcaccttgtt tgattattgc ctgcgccgat
     9541 ttgaaagata acattaaaat aaacgggaag gactattatc tggtggatac agccatttct
     9601 tttcatcaaa tgttattata tgcacgcagt tacggaattg aaagttgctg gctggctgca
     9661 ttttccgaaa agaaactaaa ggcatattta aaaattccag agaactggaa aatagtagca
     9721 atggctcctt tgggctatcc gaaagaaaaa aaaggtcttt attccaaagc agtgagcaat
     9781 tttgctaata gcaaagatag aaaaccgctg gaaagcgttg tccgctttat gtaaacaggc
     9841 aaccggttat gtctttcaat gttgatgtaa cacgatgctt gttttgactt tcaattttgc
     9901 ttttcccgta acacaatgct tgttttgac
//
LOCUS       DAOSTH010000059         9712 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_6764_length_9712_cov_16.699078, whole genome shotgun sequence.
ACCESSION   DAOSTH010000059 DAOSTH010000000
VERSION     DAOSTH010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9712
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6764_length_9712_cov_16.699078"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..843)
                     /locus_tag="PLB65_06620"
     CDS             complement(55..843)
                     /locus_tag="PLB65_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOQ78236.1"
                     /translation="MFSYSFLVYAFLGAVLSGLSLATFAPFVTLRRISYLGEALSHIA
                     FAGIALAVLAGLNLTLTTLIFVMLIAWGITSLAKRHNIQESNTITIFLSLSMALGIIL
                     ISLSKNYSFDLASYLFGNVLLVTKSEIISLTVLSLVNIAFVCFFYKELFYLTYNHTMA
                     KVYRIKTDMVNLIFMLLLAANIVINVKSAGIILVTAQLILPAVIAFNFVHRLSRAIVI
                     SVLVAIISAVIGFYLSFQFNLPTGASIVVFEAILYFLSLLYPAH"
     gene            complement(849..1577)
                     /locus_tag="PLB65_06625"
     CDS             complement(849..1577)
                     /locus_tag="PLB65_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOQ78237.1"
                     /translation="MIQIKNLQYKINGKVILEDIFLELADGEFVAVIGPNGAGKSTLI
                     KLILGLLELQTGSILIDGIEHYNWLKNNTIGYLPQYEEFDNSFPATALDIVLMGLAGQ
                     LPLGARFKRKHKQLAKDALQQTGVAHLEQELIGKLSGGELQRVFLARALVTNSKYLIL
                     DEPEASLDRPSVESFFSLLKELNNEGKTIITISHDLTTLSKYCSFLVCLNRRLHCHNQ
                     TELIDADVIHKTFGDSLRIIEKDY"
     gene            complement(1586..2500)
                     /locus_tag="PLB65_06630"
     CDS             complement(1586..2500)
                     /locus_tag="PLB65_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HOQ78238.1"
                     /translation="MKYIRWILLVALLFTLSCKKQEVSQKKPLLLATIHPYELILQQL
                     AGDEFEVSCIIPVNASPHTWSPNPADLKNINLAALILANGLGLEGNLEKNLAERKDVY
                     LEAAVLLKDIIPLSNKELTPEENPPLEENEHHHQHSQDPHLWTSPAMMIRLVGFLEKE
                     LSVRFPNSSFVFKRNARQMQEELEKVKDKIAHEREQYSNPVIVTYHNAFHYFLQEFGI
                     EELGFVQESPGKEPTPKELSLLGNIIKEHQVKAIFLEPQMDRKAGETLAQEFNLKLLM
                     LDPLGSDGKAKTIAQLIDYNWQSMKGGF"
     gene            complement(2680..4161)
                     /locus_tag="PLB65_06635"
     CDS             complement(2680..4161)
                     /locus_tag="PLB65_06635"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOQ78239.1"
                     /translation="MKIDITRKIPEYMETIIIFQEDHGSINNVDLLPSNIAKTVQTYI
                     KSEGYNFAYGEIRNLTIISGTQKQNIILLGAGNKNELNNEKIRKLFASSIRLADKLKA
                     NQVYLFMGFISPINDVALGHILTETALMVTYKFNKYKSKDKSEHEIDALHLVLSTKST
                     RHLNRGIVEGRIYAETTNLARNLVNEPANIVTPEYLAEAAKQAALQYSFSIEVFSQDK
                     LKRIKMDAFLAVGRASTHEPKLIIMRYNGNPDKKQETLALIGKGITFDSGGLCLKSSQ
                     GMLNMKSDMGGAAAIIGTMCAISAMKLKVNVVGVIAACENMISGDAYHAGDIITTMSG
                     KTIEVVNTDAEGRLTLCDAIHYAIEKEHADRIIDIATLTGAAVGALGTEISAVITNNY
                     PWLEQLKIAANFTGENIWQLPTYEDYKEQIKSEIADLKNSGGSFAGTITAGLFLKEFV
                     QDKPWLHIDIAGTALKDRESGIYNYGATGVGVRLLTTLLKSME"
     gene            complement(4191..7211)
                     /locus_tag="PLB65_06640"
     CDS             complement(4191..7211)
                     /locus_tag="PLB65_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HOQ78240.1"
                     /translation="MNKPFCCLFLLLVLCSSVFAYSFGQNKVNATPQEFSLIQTMHFD
                     IYFPAGEDQFGKTVALMAEDIYYYIKAEFSIPISSRIPIIFYATKTEFLSTNIIYSIL
                     TEGVGGFTESLHNRVAVPFEGSYGNLEEVLAHELTHAYLNAVDSHYLNVNTSLRPTSF
                     PFWFSEGLPEYLSIGGEDDYNNMYLLDMVINNTIVNLDTVDGYLAYRLGESFLTYIAE
                     QWGREKVPEYFFSIRASYNLDEATKKTFGMNFEDLENRWLYQLKRDYYPLINSHSIPA
                     ECFEQRTYHKNDGSYFNLAPRFSPDGSRYVYYSNSGGRYSIWLAGTQGLAKPQLIFKG
                     EKSAQAEEFYYFRSALSWFPDNNRIAFAAKTATGDKIHILDVKKEKIVRTITIPELKA
                     IYEADVSPDGLSLVLAAQKGVQADLFLYDLQTDKLTPLTNDSYDDTHPRFSPDGKYVA
                     YDSERKTKKVDSRYGLFSDLTRDIFTIDLQTNEIMQRTFEDYDCFTPIWAENGNYLAF
                     LSYKHSIANYEIMDLQTGKRAELTKCIAGIMNGDISLDSNYLIVSNYFDGAWDIYFDD
                     NPLINLVFEEYQQPQVYQRSDDLLQTVDLSRLDLYSKRNLAEIRKEQKSIQHNPRRPY
                     INDFENTQSDSLLIFPDYSWDNKPDTLSTPITIDKYRTKFALDNIWGGLAYSSYSGTV
                     GALELSLSDVMGNYGIGISLGISGKIEDSNIFLSVMNMKHRADYGIGVYNFYDERLYR
                     GYLAGADEYFRLRDREMGMLFIYRYPFSRFMRLEFDNRLYYVKQEWDYLPPEYVPAEH
                     WIMNVDKNTDTVYAPGLALVYDNALYGSTGPMLGWRMYYLVRKSFAKNELNYFTNYLD
                     WRSYYFFEKRYSLAFRFNAGISSGETPEMFSLDGLYGVRALNEDVDGKKMILGSAELR
                     FPLVDYLGLAFPIPLSIRNIRGSIFTDIGSAWDNNSDFQASVDGKLKDIKLGYGFGPR
                     INLGYFVLKFDISWLTDLSHISKPMYYLSLTEDF"
     gene            complement(7220..8122)
                     /locus_tag="PLB65_06645"
     CDS             complement(7220..8122)
                     /locus_tag="PLB65_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOQ78241.1"
                     /translation="MKKFYPNLRSVQIKTGIWTVVIGLILILGYLWLTGHLAGTSQYE
                     LKVSFPDVMGLEIGDKVMYRGMEVGRVKSITAEKDRVITTAKINRSIGLTEGTQFLVT
                     DSSLMGGKALSILPGNGIEPLNIKQIQLGNSPEGMMALISKASTGVEELKNTLQLITS
                     PQGILSSSQKLIENADGTVSEIGNATKEAKQELIATIDKIEQLTSSLQEVVAENKEPL
                     KNTIAESNIAIDRLSETLDSLSILSANLNITAQALKSKEGTAGLLLNDKQLYEKITTS
                     TDNLNALIKDIKENPKKYIKFSVF"
     gene            complement(8130..9425)
                     /locus_tag="PLB65_06650"
     CDS             complement(8130..9425)
                     /locus_tag="PLB65_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HOQ78242.1"
                     /translation="MFRKVFACVAVRGISFILPILIIIGSCSFVPLFADILIPMDNTQ
                     TNHLKAYGIAFAGLKESLVGRWLLNYRGGSFLFPDSGNLTSLCNIRGVRYENVTSSDV
                     AAILTEIQANNMESIALEKEPKIAVYIPPNALPWDDAVTLALEYAEIDYDRVWDDEVL
                     AGKLTDYDWLHLHHEDFTGQYGKFYGSYRNTSWYQNDVSVNEAMAAKHGFQKVWQLKH
                     AVAEKIRNYVINGGFMFAMCAATDTYDIALAAKDIDIVDATIDGDGIDPQYQSKLDFS
                     RTFAFENFTLKPNPYEYEFSDIDASDYSRLRGAEADYFQLFDFSAKYDPVPTMLTQCH
                     TNVIDGFLGQTTSFFKDKVKKNVIILAEVPGNNEVKYIHGNIGKGTFTFYGGHDPEDY
                     EHKVGDPETILELHKNSPGYRLILNNVLFPAAEKKQLKT"
BASE COUNT         3073 a         1939 c         1713 g         2987 t
ORIGIN      
        1 ttgcggcgga tcggcgtccg ccggctacac taaatttcat ttgatttatc aatctcaatg
       61 cgccggataa agtaaagaga gaaagtatag tattgcctca aagacaacaa tacttgctcc
      121 cgtaggtaaa ttaaactgaa aacttaaata aaatccaata acagcagata taatcgccac
      181 caaaacagag ataacaatag ctcgagaaag ccgatggaca aaattaaagg caattacagc
      241 cggcaaaata agctgagcgg ttaccaaaat tatccctgcg cttttcacat taattacgat
      301 attggcagct aaaagcaaca taaagatcag gttcaccata tcggtcttaa ttctatagac
      361 cttagccatc gtatgattgt aagttaagta aaacagttcc ttatagaaaa aacaaacaaa
      421 agcaatattt acaagagaca acactgtcag ggaaattatc tcgcttttgg taaccagtaa
      481 cacatttcca aagagatagc tggcaaggtc aaagctataa tttttagaaa gcgatatcaa
      541 gataataccc aaagccatac ttaaggacag aaatattgtt atcgtattag attcctgtat
      601 attatgcctt tttgccaacg aagtgattcc ccaagcaatc aacattacaa aaatcagggt
      661 ggtaagcgtt aaattcaatc ctgccaaaac agccaaagca attcctgcaa aagcaatatg
      721 cgaaagggct tctcccaaat aggatattcg ccttaaagtt acaaatggag cgaaagtagc
      781 taaggataaa cctgaaagca ctgcacccaa aaaagcataa actaaaaatg aatagctgaa
      841 catcgttatt aatagtcctt ttcaataatc cgtaagctat cgccaaaggt tttatgaatt
      901 acatcagcat caataagttc agtttgatta tgacaatgca aacgccgatt caggcaaacc
      961 agaaaactgc aatatttgga aagggtagtt aaatcgtgag agatagtaat aattgtttta
     1021 ccttcgttat taagttcttt taacagagaa aaaaaactct ctacagaggg acggtctaaa
     1081 cttgcttccg gttcatccag aataaggtat ttactattag taaccaaggc acgcgctaag
     1141 aatactcttt gcaattcacc tccagagagt tttccaatta gttcttgttc caaatgagcc
     1201 actccggttt gttgcagtgc atcttttgcc agttgtttat gttttctttt aaaacgagct
     1261 cccaaaggaa gttgtcctgc caaacccatt aaaacgatat ctaaagcggt tgcagggaag
     1321 gaattatcaa attcttcata ttgaggaagg tagccgatag tgttattttt taaccagtta
     1381 tagtgttcta tcccatcaat taagatgctg cccgtttgca attccaacaa acctaagatc
     1441 aattttatta aagtggattt tccagctccg ttgggtccaa tcacagcaac aaattcacca
     1501 tcggcaagct ccagaaagat gtcttccaga attacttttc cgtttatttt atattgtagg
     1561 ttcttaattt ggatcattcc ggctgttaga aacccccttt catactttgc cagttatagt
     1621 ctattagctg ggcaatggtt ttagcttttc catcactacc taaaggatca agcatcagca
     1681 gcttcaaatt aaattcttgg gcaagggtct cccctgcttt tctatccatt tgcggttcca
     1741 aaaagattgc ttttacctga tgttctttta ttatgttccc taaaagagaa agttctttgg
     1801 gcgtaggttc tttgccagga ctttcctgca caaaacccaa ttcctcgatt ccaaattcct
     1861 gcaaaaaata gtggaaagca ttatgatagg taacgattac tggatttgaa tattgttctc
     1921 tttcgtgggc aattttatct ttcacctttt ccagttcttc ctgcatttgc cgagcattgc
     1981 gtttgaaaac gaaagaagaa ttgggaaaac gcacacttaa ttctttttcc agaaagccga
     2041 ccaagcgaat catcatagcg ggggaagtcc ataaatgagg atcttgggag tgctgatgat
     2101 gatgttcatt ctcttcaagt ggaggatttt cctccggcgt taactccttg tttgacaaag
     2161 gaataatatc ttttagcaaa actgctgcct ccagataaac atcttttctt tccgctaagt
     2221 ttttctccag attaccttcc aatcctaaac cattagccaa aataagagct gctaaattga
     2281 tattttttaa atcagcagga ttcggagacc aagtatgagg tgaagcattt acgggaatga
     2341 tacagcttac ctcaaattca tctccagcaa gctgttgcaa aattaactca tagggatgaa
     2401 tcgtagccaa aagcaaaggt tttttctggc ttacttcctg ttttttacag gaaagagtaa
     2461 aaagtaaagc aacaagtaat atccatctaa tatacttcat tttatccctc ccaagttttg
     2521 ggtttacact atgactaatt aagtgaagaa acatttaagc tctgaaagct acatccttgc
     2581 tatgaactta ttctcctctc ctgctccacc cctcctaaac gatgagttga ggacgggtgg
     2641 agcaggagtg gaagagggga ggttagcaag gatgaacttc tattccatac tttttaaaag
     2701 agttgtaagt aaacgaactc caactcctgt tgccccgtag ttataaattc cagattctct
     2761 atcttttagg gcagtaccag cgatgtctat gtgcagccaa ggtttatctt gcacaaattc
     2821 tttcaagaaa agacccgcag tgatggttcc ggcaaaggag cctccggaat ttttcagatc
     2881 cgctatttcc gatttgatct gttctttata atcttcataa gtgggtaatt gccagatatt
     2941 ttcgccggta aaattggcag ctatttttag ctgttccagc caaggatagt tattcgtaat
     3001 aacggcagaa atttctgttc ccaaagcacc tacggcagct ccggttaggg tagcgatatc
     3061 aataatgcga tctgcgtgtt ctttttcgat agcataatgg atagcgtcgc aaagagttag
     3121 gcgtccttca gcgtctgtat ttactacttc gattgtttta ccgctcatcg tagtgataat
     3181 atctcctgcg tgatatgcgt ctccagaaat catattttcg caagcagcaa tcactcccac
     3241 aacattaact ttcaatttca tagcagaaat ggcacacata gttccaataa tggcagctgc
     3301 gccacccata tcacttttca tatttaacat cccctgagag ctttttaggc acaaaccacc
     3361 gctatcaaaa gtgatgcctt ttccaattaa agcaagggtt tcttgttttt tatccggatt
     3421 tccattatag cgcataataa ttaattttgg ttcatgggtg gaggcgcgtc ccacagctaa
     3481 aaaggcatcc atttttatgc gtttaagttt atcctgcgag aagacttcta tagagaagct
     3541 atattgcaaa gcagcttgtt tggcagcttc agcaaggtat tcaggggtaa ctatattggc
     3601 aggttcattt accagatttc ttgccagatt agtagtttca gcgtaaattc gtccttccac
     3661 aattcctcta tttaagtggc gggtactttt agtggaaagc acaaggtgga gggcatcgat
     3721 ttcgtgttcg cttttatctt tgcttttgta tttgttaaat ttgtaggtaa ccatcaacgc
     3781 tgtttcggta agaatatgac ccaaagccac atcgttgata ggagatataa aacccataaa
     3841 aaggtaaacc tgattagctt tcagtttatc tgctaaacgg atggaagaag caaagagttt
     3901 tctgattttt tcgttgttca gttcattttt gttaccggcg cccagcagaa taatattctg
     3961 tttttgtgta ccagagatga ttgtgaggtt tcttatttct ccataagcaa agttataacc
     4021 ttcactctta atgtaggttt gtacagtttt ggcgatattg gagggtagta aatctacatt
     4081 gttaatggaa ccgtgatctt cttggaagat gattatagtt tccatatatt cggggatttt
     4141 acgcgtaatg tcaatcttca ttatgaactc cttaatttat cttattgttt ttaaaaatcc
     4201 tcagtcaagc ttagataata cattggttta gaaatatggg aaagatcggt aagccaagaa
     4261 atatcaaatt tcaaaacaaa atagcccaaa ttaatcctgg gtccgaaacc gtaaccaagc
     4321 ttaatatcct ttaatttacc atcaacgctg gcttgaaaat cggagttatt atcccaagca
     4381 gaaccaatat cggtaaaaat gcttcctcgg atatttctta tactaagcgg aataggaaaa
     4441 gcgaggccta aataatctac aagaggaaag cgcaattcgg ctgagcctaa aatcattttt
     4501 ttaccatcta catcttcatt caatgcacgc acaccataaa gaccatcaag tgaaaacatc
     4561 tctggtgttt cacctgaact gattcctgca ttgaaacgaa aagctaaaga ataccttttt
     4621 tcgaaaaagt aataactgcg ccaatccaaa tagttggtaa agtaattcaa ttcgttctta
     4681 gcaaaacttt ttcttactaa gtaatacatt cgccaaccca acataggccc tgttgaacca
     4741 taaagagcat tatcataaac taaagccaaa cctggagcgt aaacagtatc ggtattttta
     4801 tctacattca ttatccaatg ctctgctgga acatattctg gagggagata atcccattct
     4861 tgttttacat agtaaagtcg attatcaaat tccaaacgca taaagcgact gaagggatat
     4921 cggtaaataa acagcatacc catttctcta tcgcgcaaac gaaaatattc atcagcgcca
     4981 gctaagtaac cacggtaaag cctttcgtca taaaaattat ataccccgat tccgtaatcg
     5041 gcacgatgtt tcatattcat aactgagaga aagatattgc tgtcctcgat ttttccagaa
     5101 atgcccaaac tgatgccgat gccataattg cccataacat cgcttaaact taattccaaa
     5161 gcacccacgg ttccactgta ggaagaatat gccaatccac cccaaatatt atctaaagcg
     5221 aattttgttc tgtatttatc aatggttata ggggtggata atgtatcagg tttgttgtcc
     5281 caagaataat cggggaaaat gaggagagaa tcactctgcg tgttttcaaa atcattgata
     5341 taaggacgac gcggattatg ctgtatggat ttttgctcct ttctaatttc tgccagattt
     5401 cttttactgt ataagtcaag acgcgataga tctaccgttt gtaaaagatc atcactacgc
     5461 tggtaaactt gtggttgctg atattcttca aacaccagat ttattaaagg gttatcatca
     5521 aaatagatat cccaggcacc atcaaagtaa ttgctaacaa taagataatt actatccaag
     5581 ctgatatcgc cattcataat tcctgcaatg catttagtaa gctcagcgcg tttaccagtt
     5641 tgtaaatcca ttatttcata attggcaatg ctgtgtttgt aagaaagaaa agccagataa
     5701 ttgccatttt cagcccaaat tggtgtaaaa caatcataat cttcaaaagt tctttgcata
     5761 atttcattgg tctgaaggtc tatagtaaaa atatcacgag taagatctga aaataaaccg
     5821 taacgactat ccactttctt agtttttcgt tcgctatcat aagctacata ttttccatca
     5881 ggcgaaaaac gaggatgcgt atcatcatag ctatcattag ttagtggtgt tagcttatct
     5941 gtttgcaaat cgtacaaaaa taaatctgct tgcacaccct tttgcgcagc caaaacaaga
     6001 gataaaccat caggagaaac atctgcttca taaatagctt tgagctctgg aatagtgatg
     6061 gttctaacta ttttctcttt ctttacatct aaaatgtgga ttttatcgcc ggtggcagtt
     6121 ttggcagcaa aagcaattct gttattatcg ggaaaccaag aaagagcgga acgaaaatag
     6181 taaaattctt ctgcctgggc acttttctcg cccttgaaaa ttaactgcgg ttttgctaaa
     6241 ccttgtgttc cagccagcca aatactgtat ctgcctcctg aatttgaata gtatacatag
     6301 cgtgaaccat ctggagaaaa acggggggcc aaattaaaat aggagccatc atttttatga
     6361 taagtacgct gttcaaaaca ctccgcagga atgctatgac tattaataag aggataataa
     6421 tctcttttta attgatagag ccagcgattt tcgaggtctt caaaattcat tccaaaggtc
     6481 tttttagtgg cttcatccaa attgtaagat gcacgaatag aaaaaaaata ctccggaact
     6541 ttttccctgc cccattgttc agcaatatag gttaaaaaac tctcgcccaa acgatatgct
     6601 aaataaccat ctacagtatc caggttaacg attgtgttat ttataaccat atctaataga
     6661 tacatattat tatagtcgtc ctcgccacca atggaaagat attccggaag cccttccgaa
     6721 aaccaaaaag gaaaggaagt aggcctaagc gaagtattaa cattaagata atggctatca
     6781 acagcattca gataagcgtg ggtaagttca tgagctaaaa cttcttctaa attgccataa
     6841 ctgccttcaa aagggaccgc tacccgatta tgtaaacttt ctgtaaaacc accaacgcct
     6901 tctgttaata tggaatatat gatattggtg ctcaaaaatt ccgtcttggt tgcataaaag
     6961 ataatcggaa tgcgacttga aatcggaata ctaaattctg ccttaatata ataataaata
     7021 tcttcagcca ttaaggcaac cgtttttcca aactgatctt cgcccgcagg aaaatagata
     7081 tcaaagtgca tcgtttgaat tagcgaaaat tcttgaggag tagcattaac tttgttctga
     7141 ccaaaactat aggcaaaaac agaactgcat aataccaata gtaaaaagag gcaacagaag
     7201 ggtttattca ttatccatcc taaaatacac taaatttgat atattttttg gggttttctt
     7261 taatgtcttt aatcaaggca ttgaggttat cggttgaagt tgttattttt tcatataact
     7321 gtttgtcgtt caataacaag ccagctgttc cttctttact ttttagagct tgggcagtta
     7381 tatttaaatt ggcggaaagg atgcttaagc tatccagggt ttcagataat ctgtctatgg
     7441 caatattgct ttcggcaata gtatttttga ggggttcttt gttttccgct accacttctt
     7501 gcagagaaga ggttaactgt tcaattttat cgatagtagc aattaattcc tgtttggctt
     7561 cttttgtggc atttccgatt tcgctaactg tgccatcagc attttcaatt aacttttgcg
     7621 aactggaaag tataccctgt ggtgaagtta tcagctggag ggtatttttt aattcttcca
     7681 caccagtgga ggcttttgaa atcaaggcca tcataccttc tggtgaattt cccagttgga
     7741 tttgttttat gtttagaggt tcgattccgt ttccgggtag gatgcttaag gcttttcccc
     7801 ccattaaaga actatctgta accagaaatt gagtgccttc agttaaccca atgctgcggt
     7861 ttattttggc agtagtaata actctatctt tttcggcagt aatgcttttc actcggccaa
     7921 cttccattcc gcgatacatt actttatctc caatttccaa acccattaca tcgggaaaag
     7981 aaactttaag ttcatattgt gaagttccgg ctaaatgacc tgtcagccat aaataaccca
     8041 aaattaaaat caaaccaata actacggtcc agatgccggt ttttatctga acactacgca
     8101 agttaggata aaatttcttc atactttttt tatgtcttta gctgtttttt ctctgctgct
     8161 gggaagagga cattatttaa tattaaacga taacctggcg aatttttatg taactccaaa
     8221 atggtctctg gatcgccaac tttatgttca taatcctcag gatcgtggcc accataaaaa
     8281 gtgaatgttc ctttgccaat attaccatgg atatatttaa cttcattgtt gccagggacc
     8341 tcggctaaaa taattacatt cttcttcacc ttatctttga aaaaagaagt ggtctgaccc
     8401 aaaaagccgt caataacatt ggtatgacac tgagtaagca ttgtgggaac aggatcatac
     8461 tttgcgctga aatcaaaaag ctgaaaataa tcggcttctg cacctcgcaa acgagaataa
     8521 tcactggcat caatatcgga aaattcatat tcataaggat tgggcttcaa agtgaaattt
     8581 tcaaaggcaa aagttcgaga aaaatccaat ttactttgat attgagggtc tattccatca
     8641 ccatctattg tggcatctac aatatctata tctttggctg ctaaagcaat atcataagta
     8701 tccgtagcag cacacattgc aaacataaaa ccaccattta ttacatagtt acgaatcttt
     8761 tccgctactg cgtgtttaag ctgccaaact ttttgaaaac cgtgttttgc tgccatcgct
     8821 tcatttacgc tcacatcatt ctgataccaa cttgtattac gataagaacc gtaaaatttg
     8881 ccatattgac ccgtaaaatc ctcatgatgc aaatgcagcc aatcataatc cgtaagcttg
     8941 cctgccaaaa cttcatcatc ccaaacccgg tcataatcaa tctcggcata ttctaaagct
     9001 aaagtaactg catcatccca gggaagggca ttgggaggaa tataaacagc tattttgggc
     9061 tctttctcta aagcaatgct ttccatatta ttcgcttgaa tttctgttaa aatagcagcc
     9121 acatcggaag aagttacatt ttcataacgc acgccacgaa tgttacataa agaagttaaa
     9181 ttgcctgaat caggaaagag aaaacttccc cctcgataat ttaacagcca cctgccaact
     9241 aaactttcct taagacccgc aaaggcaatg ccataggcct ttaagtgatt ggtttgcgtg
     9301 ttatccatcg gaattaatat atctgcaaat aaaggcacaa aactacaact gccaattatt
     9361 attagaatcg gcaaaataaa gctgataccc cttacagcaa cacacgcaaa cacttttcta
     9421 aacaaggatt taatcctcct catttccctt ttgcttatta taagccattt ctgtgccata
     9481 aaaggaaatt atctgttccg ctgtcaagat aaatctctgt tgctggtttt tttaccctga
     9541 aaaacacttt aattagtcaa aagctctgaa agccaatata aaagaataaa tacatagagg
     9601 acgtagcatt caaaacttca taaattctgt caaaactctg atagcgaatt taaaagcata
     9661 aatatctaga tagaaataag agttttatat tgaaagaaaa agctccctat tg
//
LOCUS       DAOSTH010000060         9647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_6826_length_9647_cov_11.068495, whole genome shotgun sequence.
ACCESSION   DAOSTH010000060 DAOSTH010000000
VERSION     DAOSTH010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6826_length_9647_cov_11.068495"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(219..1430)
                     /locus_tag="PLB65_06655"
     CDS             complement(219..1430)
                     /locus_tag="PLB65_06655"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HOQ78243.1"
                     /translation="MKNILTNVINPVSCEVIQFLPEQVISIEGSKITAIIPKQEFQGI
                     VDEDLSHQFALPGFIDLHIHLSQYYIRGLYEPALLPWLNKYVFPEEARSKDNEYAQKL
                     SRDFFSALLKDGTTTAVIYTAPFFEACNTAFEIASEIGIRALIGMTLMDKNCPEPLKQ
                     DSHKAIEDSFLLYEKWHNKNAKLDYVFTPRFAPTCSMELMQEIGKFAKKHNAWIQTHL
                     SENKAELNLVKELFGFDNYTLVYEKAGLLSEHSIFAHCIHLDDSELQLLAENKCKIAH
                     CPDSNFFLKSGEFPIKRIEEKGIAYGLGSDVGSGTTINMLYHTKMMNYRQSWEPVSAA
                     KALYYITLGSARLLGLDSEIGSLETGKEADIVFIEPAEDFLVDDNIISKLVFCESDFT
                     ITHTLVGGKEL"
     gene            complement(1433..4096)
                     /locus_tag="PLB65_06660"
     CDS             complement(1433..4096)
                     /locus_tag="PLB65_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HOQ78244.1"
                     /translation="MKYLHLIILILFTVSIFAITNEEIRFLCDKEQFQLLAKHYDTIQ
                     KKIKTGDEEDLDTVLYYAHRAYLQDIAKQCHKRLAKDYHSLEDALQWLFLCESSEVDS
                     LTLYNDIDSITQALTDPLDKNVWLYYTKNCSEEELFSQVAASEKYNTIIEAMAKDLID
                     EISIEQSDSLALLKLQEFDNRYPNSQYRSIALYYKLYNLANRKNWAGLIESLPPLENL
                     DPPSAYISSLFLLSPTFRRDFTGTEDLLDLSDKYLAKAMSDKEQTLLYDLYSAEDWKA
                     RVLQQQVKLQYYRLVLPYGLYGDEVETPVLDKSNLKQQKELLALQNKVHFTNNNRGEQ
                     AEEHFWMAKVLLLTGKKTDKQKAANQLTQCLILGSPRNRYDVEAMAIIQNLHRDLKIK
                     EEPLQWMQKIANYKGICFEDLSDKANLNKKGYTRVALADYNSDGLVDILFNGKYLYKN
                     TGNMQFSEVTDSVGLTDLHSNGGIFADFNKDGLLDFVSYSHSSVGRGDQLMKNIDNTS
                     FVNVNERAGDIDDTYPTEAVAWIDINKTGYPSLYAANYEIWQKRAGFPDFFWENKDSF
                     FTDQSYNYGFREPYYSDKPGLPGRGVAPADFDNDGKQEIYVTNYRLSRNFCWKQNDDI
                     FVDVAALYGLDGIYKNGYYGHSIGADWGDYDNDGDLDLFVANLAHPRYIDISDISMLL
                     RNDGLTYRVVENDTIYFWQFTNVTKQAGITYDELHSDPLFFDADNDGYLDLFITSVYD
                     NERSYLYHNKGDGTFEDITWLSGARVYNGWGNASADLNRDGLIDLVVGSGNGAKILQN
                     KTVTNNNALYVKPVWKNDIVELVTDTNNYDKVPNTPAFGTRVEVTIKRPFHKQKTLIR
                     ELCSAKGTGSQNAPELHFGINKGKVVNIRIK"
     gene            complement(4099..4974)
                     /gene="hslO"
                     /locus_tag="PLB65_06665"
     CDS             complement(4099..4974)
                     /gene="hslO"
                     /locus_tag="PLB65_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOQ78245.1"
                     /translation="MNKDYLIRGTAHHNNFRIFAVQSTNCVQTVRDLHDLSPLATLLL
                     GRMISAAAMLSWDLKDEGSEVTMRIDCEGDIKGAIVICTQDGRLRGYTKKPQLFYNNR
                     EDNFKVGKAIGKGTLTISRDLKGIRSYLSTTELISGEIAEDLAYYFQQSEQIPTAVNL
                     GVLIDKTAKVRAAGGFIIQQLPFADEKTADIINENLNLTPNLSDLMDMGLSIPDVLSR
                     FVFKGIQWQINEEKDIAYHCNCSSELFSKALLLLGKEELQTLKEGIKPVCLYCNKVYD
                     FTAEDIQQLIKQLQE"
     gene            complement(4967..6508)
                     /locus_tag="PLB65_06670"
     CDS             complement(4967..6508)
                     /locus_tag="PLB65_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HOQ78246.1"
                     /translation="MAKEIPVQLCNDLYTMLKCNCLFILLLLFIPLLWGAQFRDGELY
                     LEINLAASPTLSITSERKDEKITVSEMDGLISRSFDSDVYFFIVNPEKTVRYAILDYT
                     EIISEPLDGKNAVVRNFFAWENSHLKIKTELQHFQAESFNTEEEALQYASLYNIPLDK
                     IKEVQLINTTVQIQDAKGNICFMETPLKIHSDTDLYFNSNSYGYNGDFILKTVKKKIV
                     LNQFLPLENYLAGVVQNEIGNNADMEALKAQAVAARTHALNLLLNNKHKVDGYDLCNT
                     THCQVYKGKYLQDEHILRAVYGCASEVLLINDNLAEATYHSCCGGKTDASSIIWKGKP
                     VDHLNGVICISEAEEYDLTKESDTRRWIDEQLSTEGMSSWERGALSWHKSIPLKQIAD
                     NAGLAYINRIEIHKRGNSGRITNMKLIGNETVTYNNEYQIRYIFGNLLSSFFYIEGNY
                     TENNGITVIIPKASVNVKGRGAGHGVGMCQVGALKKAREGDNYHDILQHYYPTTTINK
                     DWMDE"
     gene            complement(6474..8510)
                     /gene="metG"
                     /locus_tag="PLB65_06675"
     CDS             complement(6474..8510)
                     /gene="metG"
                     /locus_tag="PLB65_06675"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOQ78247.1"
                     /translation="MKYLITSALPYANGRLHIGHIAGAYLPADIFVRYLRLKDEDVIY
                     ICGTDEHGTPISITADKEGITPQEVVNRYHQSIKEAFDGIGIEFDNFSGTARPPHYKL
                     ASEFFLQLYNKDFIKPKITLQFYCEHDKRYLPDRYVEGLCPRCGASNARGDQCDKCGQ
                     IYETTTLQEPKCKICGSTPVIKETKHWFLQLDGFRDKLKRWLSTKDYWKENVRNFIFG
                     LLDQGLVERSITRDLSWGVPVPLPEAKGKVLYVWFDAPIGYISSTVEWAEKIGQPEKW
                     KEYWLNPETRLIHFIGKDNIIFHSLIWPAMLMGQDKKYCLPYDIPANEFMNLEGEKIS
                     TSKNWAIWVDEFLQDFEGEYLRYYLAVNAPEKQDSDFCFKDFQSKINTDLNNTLGNLA
                     NRVFAFANKNFEGKITACSLSKSAKLIIDEADAILKEIEECYLDYQVKKNTRLIMDIA
                     RLGNRYFDENKPWAILKENREAVNETLYVCANLLAKISVALFPILPSSMLKLREMMGL
                     SDQFLFNEAYNILDDIKLTDIKPLFHKIEDKEIEAQIAKLHLQTKINEGDKVEFATSK
                     IAPLKELISYDDFSKLDFRLAKVLTAEKIPNTDKLLKLQVDIGIETRELVAGIAADYE
                     PQDLIGKTILMLVNLEPRKIRGINSQGMILAANANGKLLVLNPDGEGNPGSVVQ"
     gene            complement(8507..>9647)
                     /locus_tag="PLB65_06680"
     CDS             complement(8507..>9647)
                     /locus_tag="PLB65_06680"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOQ78248.1"
                     /translation="NIDLIRNIAHKLACEYHKGDALVLVVSAMAKTTDYLFNLAYGIS
                     QHPSRRELDMLLTAGERISMSLLSLALMEEGIPSISFTGSQSGIITDEKHGNAQILKV
                     SAFRIQEELNKGKVVIVAGFQGVSISKEITTLGRGGSDTSAVALACYLGADKCEIYTD
                     VDGVYTADPKLVANPQLLKYISPELMLTLCYNGSKVLHPRAVEFAIKYGVEVEVKSSF
                     TFAPGSLITRIKIPNNEEDKMEERKVTAISYKDKLIRYIIPVSEQVSDMLTLCNYEIY
                     KSFINAGFLELFVETKYETEVDYFLQDRGIEPKNKESNLAFVNLVGLGLGHDPAFLAS
                     VIENSKNYNILRSYNNEQSIELLLPNNHLSEVVQKLHTIYIEEKQ"
BASE COUNT         2965 a         1877 c         1562 g         3243 t
ORIGIN      
        1 tctatacaac agttcgttat aacttatcca catcgttttt ttgtgggaat gcctagaaag
       61 taggaagccc atcccataga ctataaattt tccatccttt atatagtgta gctatattat
      121 ttatcataga acggttttac aagacagcaa aaatagtact taactgcttt gattcatatt
      181 tatggctctt ctgctatttc gggaaccgaa aaaaacatct aaagttcttt gccgccaaca
      241 agtgtatggg taatagtaaa atcactttcg caaaagacaa gtttagaaat tatattatcg
      301 tctacaagaa aatcttcggc tggttcgata aagactatat cggcttcttt tcctgtttcc
      361 aaagagccta tttcgctatc caaaccaagt aatcttgcac tgcctaaagt tatataataa
      421 agtgccttag cagctgaaac aggttcccag ctttggcggt aattcatcat ttttgtatga
      481 tataacatat ttattgtagt tcctgatcct acatcgcttc ctaaaccgta tgcaattcct
      541 ttttcttcaa ttctttttat tgggaattct ccacttttca gaaagaagtt ggaatctgga
      601 cagtgagcta ttttacattt attttcagca agtaattgta attcactatc atctaaatga
      661 atacagtgag caaagatact gtgttcgcta agcagaccag ctttttcata aaccagagta
      721 taattatcaa aaccgaacag ttctttcact aagttcaatt cagctttgtt ttccgagagg
      781 tgagtttgaa tccaggcatt atgtttttta gcaaatttac caatctcttg cattagttcc
      841 attgaacagg ttggggcaaa acgaggagta aatacataat caagtttagc atttttgtta
      901 tgccattttt cataaaggag gaaactatct tcaatcgctt tatggctgtc ttgtttaaga
      961 ggttccggac aatttttatc catcaaggtc attccaatta gagcccggat tcctatttct
     1021 gaggcaattt cgaatgctgt attacaggct tcaaaaaaag gtgcagtata aattactgca
     1081 gtagttgttc catctttcag caaggcagaa aaaaaatctc tactaagctt ttgggcatat
     1141 tcattatctt tagaacgagc ttcttccgga aaaacatatt tatttaacca agggagtaat
     1201 gcgggctcat ataaaccgcg aatataatat tgacttaaat gaatgtgcaa atcaatgaaa
     1261 cctggtaaag caaattgatg agaaaggtct tcatctacaa ttccctgaaa ttcttgcttg
     1321 ggaattatag ccgttatttt actaccttca atactgataa cttgttctgg tagaaattgt
     1381 atcacttcgc aggaaacagg attgataaca ttggtcagga tgtttttcat atttacttta
     1441 tccttatgtt aactactttt cctttgttta taccaaaatg caattccgga gcattttgag
     1501 agcctgttcc tttagcactg caaagctccc tgattaatgt tttttgctta tgaaaaggtc
     1561 tttttatagt tacttccact cttgttccaa aagcgggggt attgggaact ttatcataat
     1621 tatttgtatc ggttaccagc tctacgatat catttttcca aactggtttt acataaagag
     1681 cgttattatt ggttactgtt ttgttttgta atatcttagc tccattacca ctcccaacca
     1741 caagatcaat taacccatcc ctgtttaaat cagcagaggc atttccccaa ccgttataaa
     1801 ctcgtgctcc cgaaagccag gttatatctt caaaagtgcc atcgccttta ttatgataca
     1861 ggtagctgcg ttcattatcg taaaccgaag taatgaataa atccagatag ccatcgttat
     1921 cagcatcaaa aaatagagga tcactgtgca attcatcata agtaattcct gcttgtttag
     1981 ttacattggt aaactgccaa aagtaaatag tgtcattttc tactactctg taagttaaac
     2041 catcgtttct aagcagcata ctgatatcgg aaatatctat ataacgggga tgagccaaat
     2101 ttgctacaaa taaatccaaa tcaccatcgt tatcataatc gccccaatct gctccaatac
     2161 tgtgtccata atagccattt ttatatattc catctaaacc gtataaagct gctacatcca
     2221 cgaaaatatc atcattttgt ttccagcaaa aattacggct caaacgatag ttggttacat
     2281 aaatttcctg tttgccatca ttatcaaaat cagcaggagc tacacctctt ccaggcaagc
     2341 caggtttgtc tgaataatac ggttcacgaa atccataatt atagctttga tcagtaaaaa
     2401 agctatcttt attttcccag aagaaatcag gaaaacctgc ccgcttttgc cagatttcat
     2461 aattagcagc atagagtgat ggatatccag ttttgttaat atcaatccaa gcaacggctt
     2521 cagttggata agtatcatca atatcaccag cgcgttcatt aacatttaca aagctggtat
     2581 tatcaatatt tttcattagt tgatcgcctc tcccaaccga ggaatgactg taggaaacaa
     2641 aatccagtaa tccatctttg ttaaaatctg caaagatgcc tccattgctg tgtaaatcgg
     2701 ttaagcctac gctatctgta acctctgaga attgcatatt accggtattt ttatatagat
     2761 attttccgtt gaataggata tctactaacc catcagaatt ataatccgct aaggcaacac
     2821 gagtgtaacc ctttttattt aaatttgcct tatcacttaa atcttcaaaa cagatacctt
     2881 tatagttagc tattttttgc atccattgca gcggttcttc tttaatctta aggtcgcgat
     2941 gcaagttttg tataattgcc attgcttcaa catcataacg attacgaggt gaacctaata
     3001 ttaaacactg ggtaagttga tttgctgctt tttgtttatc tgttttcttg cctgtaagaa
     3061 gtaatacttt tgccatccag aaatgttctt ctgcttgttc accacgatta ttattagtaa
     3121 aatgaacttt attttggaga gcaagtaatt ccttttgctg tttcaggttg cttttatcca
     3181 aaaccggagt ttccacttca tctccataaa gaccataggg gagaactaaa cgataatatt
     3241 gcaattttac ctgttgctgt aaaaccctgg ctttccaatc ctctgctgaa tataaatcgt
     3301 aaagcagggt ctgttcttta tctgacattg ctttagccag atatttatca gataaatcta
     3361 agagatcttc tgtgcctgta aagtcccttc gaaaagtagg tgatagtaaa aataaggatg
     3421 atatataggc agaagggggg tctaaatttt caaggggagg cagggattca attaaaccag
     3481 cccaattttt acggttggca aggttatata gtttataata tagggctata gaacggtatt
     3541 gggaatttgg gtatctgtta tcgaattctt gcagttttaa cagagcaaga gaatcacttt
     3601 gctcgatact aatttcatca atcagatcct tcgccattgc ctcaataatt gtgttatatt
     3661 tttctgaagc agcaacttga gagaaaagtt cctcttcact gcaatttttg gtataatata
     3721 accagacatt tttatccaga ggatcggtta gagcttgagt tattgaatca atatcgttat
     3781 atagggtaag ggaatctact tcagatgatt cacacaaaaa aagccattgc aaggcatctt
     3841 ccaaagagtg atagtctttt gctaaacgct tatgacattg tttagcaata tcttgtagat
     3901 atgctctatg ggcataatag agaaccgtat caaggtcttc ttcatcaccc gttttaatct
     3961 ttttttgaat tgtatcatag tgtttggcaa gtaactgaaa ttgttccttg tcacataaaa
     4021 atcttatctc ttcattggtt atagcaaaaa tgctcacagt aaacagaata aggataatta
     4081 aatgtaagta cttcattgtt attcctgtag ttgtttaatt agttgttgaa tatcttctgc
     4141 agtaaaatca taaactttat tgcaatatag acatacaggt ttaatgcctt ccttcagggt
     4201 ttgcaattct tcttttccta atagtaataa tgccttgctg aaaagttcag aactgcaatt
     4261 acaatggtag gcaatatctt tttcttcgtt aatctgccat tgtattcctt taaaaacaaa
     4321 gcgggaaaga acatctggga tggaaagtcc catatccatt aaatcgctca agttaggtgt
     4381 tagattcaaa ttttcattta taatgtctgc tgttttttcg tcagcaaaag gtaattgctg
     4441 aataataaaa ccccctgctg cacgtacttt ggctgtttta tcgattagaa cacctaagtt
     4501 tacagctgta ggaatctgtt ccgattgttg aaagtaatag gcaaggtctt cagcaatttc
     4561 accactgata agttcggttg tgctaagata tgatcttatc cctttcaggt ctctacttat
     4621 agttaatgtc cccttaccaa tggctttacc tactttaaaa ttatcttctc gattattata
     4681 aaagagctga ggcttttttg tgtaacctct taagcgtcca tcctgagtgc agataacgat
     4741 tgcacctttt atatctcctt cgcaatctat gcgcattgta acctcactac cttcatcttt
     4801 taaatcccag gaaagcatcg ctgcagcaga aatcatcctt cctaaaagta aagtggcgag
     4861 aggagaaaga tcatgtagat cacggacagt ttgaacacag tttgtacttt gcacagcaaa
     4921 aatccgaaaa ttattatgat gtgcagttcc gcgaataaga taatctttat tcatccatcc
     4981 agtccttatt tatagtcgtt gtaggatagt aatgctgtaa aatatcatga tagttatctc
     5041 cctcacgtgc tttttttaaa gctccaacct gacacattcc aactccatgt ccagcacctc
     5101 taccttttac attaacactt gctttgggga taatgacagt aattccatta ttctctgtgt
     5161 aattaccttc aatataaaaa aacgaagaaa ggagattacc aaatatatac ctgatttgat
     5221 actcgttatt ataagtaact gtttcattac caattagttt catatttgta atccgtccgg
     5281 aatttcctct tttatgaatc tcgattctat ttatataggc aaggcctgca ttatcagcta
     5341 tctgttttaa tggtatactt ttatgccagg aaagagcacc gcgttcccaa gaactcatcc
     5401 cttccgtaga aagctgctca tcaatccatc tgcgagtatc tgattcttta gtcagatcat
     5461 attcttcagc ttcgctaatg caaattactc catttagatg gtcaaccggt tttcccttcc
     5521 aaataatact ggaggcatct gtttttcctc cacagcaact atgataagtt gcctcagcaa
     5581 gattatcatt tattaataag acctcagaag cacaaccata aacggctcgt agaatgtgtt
     5641 catcttgtaa atatttaccc ttataaacct gacaatgagt tgtgttgcac aaatcataac
     5701 catctacctt gtgtttatta ttcagcagta aatttaaggc atgagttctt gctgcaactg
     5761 cttgtgcctt aagtgcttcc atatctgcat tgttacctat ctcattttgc accacaccag
     5821 caagatagtt ttccagaggc aggaattgat tcaaaacaat cttcttttta acagttttca
     5881 agataaaatc gccattatag ccgtaactat tcgaattaaa atatagatca gtatcggaat
     5941 gtattttcaa aggtgtttcc ataaagcaga tattaccctt ggcatcttga atctgtactg
     6001 tagtattaat aagctgcact tcttttattt tatcaagggg gatattatat aaagatgcat
     6061 actgaagagc ttcttcttct gtgttaaaac tctctgcttg aaagtgctgc aactccgttt
     6121 taattttcaa atgcgaattt tcccaggcaa aaaaattccg cactacagca tttttaccat
     6181 ccaagggttc agatattatt tcagtatagt ctaatatagc ataacgaacc gttttttcag
     6241 gatttactat gaaaaaatag acatctgaat caaagctgcg agaaatgagt ccatccattt
     6301 cagaaactgt tattttttca tctttccttt cagaagtaat acttagagta ggagaagcag
     6361 ccagattaat ctccaaatat aattctccat ccctaaattg agctccccaa agtaggggaa
     6421 taaaaagtaa cagaaggata aataagcagt tacacttcag catagtgtac agatcattgc
     6481 acaactgaac cgggatttcc ttcgccatca ggattaagga ctaataattt cccgttcgca
     6541 ttggcagcta aaatcatccc ttgagagttt attcctcgta ttttacgggg ttccaagttt
     6601 accaacatta aaatagtttt gccaattaaa tcttgaggtt cataatcggc tgcaataccg
     6661 gcaaccaatt cgcgtgtttc aattccaata tccacttgta gttttaataa tttatcggta
     6721 ttgggaattt tttctgcagt caaaaccttt gccaagcgaa aatcgagctt agaaaaatca
     6781 tcatagctga ttaattcctt caatggagct atcttggaag tggcaaactc tactttatcg
     6841 ccttcattga tttttgtttg taaatgcaat tttgcaattt gtgcctcaat ttccttatct
     6901 tcgattttgt gaaagagggg cttaatatct gttaacttta tatcatccaa tatgttatat
     6961 gcttcattga acagaaattg atcgcttaac cccatcattt ctctaagttt aagcatagaa
     7021 cttggtaaaa tgggaaataa ggcaacagat atttttgcca gcaaattagc gcaaacatat
     7081 aaggtttcgt ttactgcttc tctattttct ttaaggatag cccagggttt attttcatca
     7141 aaatagcggt ttcccaagcg tgctatatcc attattaaac gggtattttt cttaacctga
     7201 tagtccagat aacattcctc tatttctttt aagatggcat ctgcttcgtc gattatcagt
     7261 ttagccgatt tacttaacga acaggcagtt attttccctt caaagttttt attggcaaag
     7321 gcaaagactc tattagctaa attgcccagc gtattattta ggtctgtatt tatcttactt
     7381 tgaaaatcct taaaacaaaa atcggaatct tgcttttcag gagcattaac tgctagataa
     7441 tatctcagat attcaccctc aaaatcttgc agaaattcat caacccaaat tgcccaattt
     7501 ttacttgtag atattttctc tccttccaaa ttcataaatt cgttggcagg aatatcataa
     7561 ggcaggcaat attttttatc ttgtcccatc aacattgcag gccaaattaa agaatgaaat
     7621 atgatgttgt ctttaccaat aaaatgaatc aagcgagttt caggatttag ccaatattct
     7681 ttccattttt caggttgacc aattttttct gcccattcaa ctgtagaaga aatataccca
     7741 atgggagcat cgaaccatac atatagaact ttaccttttg cctctggaag aggaacagga
     7801 actccccaag atagatctcg agtaatggaa cgttcaacta aaccttgatc caataatcca
     7861 aatataaaat tccgcacatt ttctttccag taatctttcg tagaaagcca tctttttaat
     7921 ttatcgcgaa aaccatccag ctgcagaaac cagtgttttg tctctttaat tacaggagtt
     7981 gaaccacaaa tcttacattt aggctcttgc aatgttgttg tttcgtaaat ttgaccacat
     8041 ttatcacatt gatcacctct ggcattactg gctccgcaac gaggacataa tccttccaca
     8101 tatctatcgg gtaaatagcg tttatcgtgt tcacaataga attgtagcgt aattttgggc
     8161 ttgataaagt ccttattgta taattgtaaa aagaactctg aagcaagttt atagtgtggt
     8221 ggacgcgctg ttccgctaaa attatcaaat tcaataccaa taccatcaaa tgcctcttta
     8281 atgctttgat gataacggtt tactacttct tgaggtgtaa ttccttcttt atcagcagtt
     8341 attgaaatcg gagtgccatg ttcatctgtt ccacaaatgt aaatcacatc ctcatctttt
     8401 aaacgcaagt aacggacaaa gatgtcagct ggtaaataag ctccagcaat atgtccgata
     8461 tgtaatcttc cattagcata gggtagagca ctggtaatta agtatttcat tgcttttcct
     8521 caatataaat tgtatggagt ttttgaacaa cctccgaaag atggttgtta ggcaataaaa
     8581 gttctatgct ctgttcgttg ttataactgc gtaaaatatt ataatttttg ctgttttcta
     8641 taaccgaagc taaaaaagcg gggtcgtgtc ctaaacctaa accaacaaga tttacaaaag
     8701 ccaaattgct ttctttgttt ttgggttcta tacctctatc ttgtagaaaa taatcaactt
     8761 ccgtttcata ttttgtctct acgaaaagtt ccagaaagcc agcattaata aaactcttat
     8821 atatttcata attacataga gttagcatat ctgaaacttg ctcgcttaca ggtataatat
     8881 aacggattaa tttgtcctta tacgaaatag cagttacctt gcgttcttcc attttatctt
     8941 cctcattatt cgggattttt atacgggtga tcaacgaacc aggagcaaaa gtaaaagagg
     9001 atttaacttc cacctctaca ccatatttaa tagcaaattc aacagcccgg ggatgtaaca
     9061 ccttagaacc attataacat agggttaaca ttagttctgg actgatatat ttcagcaatt
     9121 gtggattagc cacaagctta ggatcagcag tataaactcc atctacatct gtatagattt
     9181 cgcatttatc agctcccaaa taacaagcta atgctactgc agaagtgtca gaacctccac
     9241 gtcctaaagt tgttatttct ttgctaatgc taacaccttg aaaaccagcc acgataacaa
     9301 cttttccctt attcagttct tcctgtatac gaaaagcact aactttcaat atctgagcat
     9361 taccatgctt ttcatcagta ataattccac tctgagaacc agtaaaagag atagaaggaa
     9421 ttccttcttc catcaaagct aacgataata aactcatact gatacgttcc ccagctgtca
     9481 aaagcatatc cagttcacga cgcgatggat gctgggaaat cccataagct aaattaaata
     9541 aatagtctgt tgttttagcc attgcagaga caactaaaac aagagcatca cctttatggt
     9601 attcgcatgc caatttatga gctatgttgc ggattaaatc aatgtta
//
LOCUS       DAOSTH010000061         9637 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_6844_length_9637_cov_15.107180, whole genome shotgun sequence.
ACCESSION   DAOSTH010000061 DAOSTH010000000
VERSION     DAOSTH010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9637)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9637)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9637
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6844_length_9637_cov_15.107180"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..1196
                     /locus_tag="PLB65_06685"
     CDS             18..1196
                     /locus_tag="PLB65_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HOQ78249.1"
                     /translation="MKSNLSYYRCIECGKEFDPNELHYLCDECGRDYLPGMPLKGVLE
                     AIFNYAEIGRRWKERPDPLLFSAVNPEFYPPLPVGNTPFFRTEIMGLESERMASSSYP
                     IGIKFDGLNPSGSYKDRATQLVVADALQKGITEIVTASTGNAACSLACLGASARLKIV
                     IFAPESAPPAKLIQIQVHNAELHKVQGSYDDAFKAALEYTKTHNCMCRNTGYHPLTID
                     GKKSAGLEIYIQNGFKVPDWIIIPVGDGVILTGIYKAFVDLQRAGIIQKLPQLLCVQA
                     ESSDAITSYWETGRYKDAENPITIADSIKVKTPALAHWSVKALKETRGKCIRVSDFEI
                     QEAQLKLAAETGVFAEPSSVATLAGLQKALAKKWINSNEDIVLLITGHGLKDPGAVKI
                     "
     gene            1193..2287
                     /locus_tag="PLB65_06690"
     CDS             1193..2287
                     /locus_tag="PLB65_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase family protein"
                     /protein_id="HOQ78250.1"
                     /translation="MKATGIYDFHVHIGETIGGHLLADSWKSFNRLYEHNGLEGIGVF
                     VTESPNESLSAKLSRMRKASRSFSGKVFWHLTPRNLDMRKLEIIFKNDTDFKLYTTYR
                     ESGLYQSYESIEHFMIDMPNPKKRLLIHCEDDEIISEYSERYPFHKPSDHCLRRPEKA
                     EIRAVDKVLNLSIKHHYPIHLVHISSPQAALLIQQAKKEADYITCETTPHYLLLNEEV
                     LNDLEGHRWLCSPPLRSENSRGLLLELLQDGIFDIIASDHCAFPTETKDIGALYPAET
                     PMGIAGSGVLFSLLAEQLVATGKLSMDQLFTYISINPARLMGFNIEEDKLNCERLGAP
                     IPVVPSWANTPNPWLDFWSHYELRRHHNEV"
     gene            2277..4544
                     /locus_tag="PLB65_06695"
     CDS             2277..4544
                     /locus_tag="PLB65_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HOQ78251.1"
                     /translation="MKFNNAFRNDAIAKVTGRAKYADDYKLPNMLYAVPLHSQVASAI
                     INNIDSTEALKQKGVVAVYTAKDIPGNIKYGQIIMDCPTLVNKRIRSAGDVLALIVAE
                     TREIAISALPLIKVELSELPAVFDPEEAIKENSPIVNPDFGSNICNYHKVRTGDIDKG
                     EKEADLIIEQEFNTSRVEHSYLEPETALCFLRQDGVLEVLGSMQHPFSTRRFVASTLG
                     CKLSEVEVKNIPMGGGFGGKDDTAALVCARAALCAYLTKRPVKLTYTREMSMQESYKR
                     HPYKLQYKMGLKNNGKIVFMKVRIVADGGAYCSVTPWVTWRSTVQSCGCYEVPNVQSD
                     VYGVYTNNIFSGAFRGFGSPQVNFAIEQLVEMAAERLNMDATELRKINMVHQDSVTIT
                     QQKLNNHTVSLNQVMESVLTEIDYANKVKKCSFGKPEIEDWYGIGYAICYRGVSLGAE
                     GTDMNSAIINIQPDGSVLLETGVHENGQGAQSAMILIASEELGIPKELIRYRLPSTSN
                     IPDGGSTVASRGTIMGGSSVINAAKIIKERLCAGVEKTYNITDCTYKDGSVYSAQGNV
                     IADYFEAVRLCYEHQIYPYAFGVFSAPPISWDENTGHGNAYFSWIYSCQAVELKVNNR
                     TGKITLMNLVAAHDIGKAINPALLEGQIYGGMAMGAGYALYENFPLINGKPQVSNFNK
                     YYVLRSTDLPEMTAIIIENADPNSPSKTKGIGEPALEITAPAIANALYRATGKRFTDL
                     PMAQQVKDYVQGRQK"
     gene            4541..5008
                     /locus_tag="PLB65_06700"
     CDS             4541..5008
                     /locus_tag="PLB65_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOQ78252.1"
                     /translation="MKITVNDIEYNVPKEYAETKLSTYLRENLKLTGTKIGCDIGMCG
                     SCTVLVNYQPRRSCLLKLKDVCDKQIITIEGLANPDGTLHPVQQAFIDAGAIQCGFCT
                     PGMILTAYAFLLKNKKPTREEARNVMKGNICRCTGYQQIIDAVMLAAERMNKL"
     gene            complement(5410..5604)
                     /locus_tag="PLB65_06705"
     CDS             complement(5410..5604)
                     /locus_tag="PLB65_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78253.1"
                     /translation="MKKVLLITLLAMLLISTLVTGCKQKAEEPQVEETAPIEETTPQV
                     DTTIAPTQGETPATQTPTGK"
     gene            complement(5811..7487)
                     /locus_tag="PLB65_06710"
     CDS             complement(5811..7487)
                     /locus_tag="PLB65_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HOQ78254.1"
                     /translation="MCCSPKLRYYLIYILFVFVSPLLALTIGKVNFVGISGIEESELI
                     KASGIKSGQEYNPELINNATAKLYKYFQDMGRYFIVISAPDLIPEEGETLSFLFHLQE
                     KAPSDEMQIRFAGMQYFSEAKLYQFLLLSPERNYHLNQLPGIMQQVLSLYNSRGYLFA
                     KVQLDSLVLEDKLTAYIGIEEGKPLRVNNYIFRGNKVTKDKTLLQLSGLDNLDIITLE
                     SITQAQENILRKSYIRQCFIEPINDNTLLINVEEGKMTYLEGVLGMNRINNTTKLSGQ
                     IRLQFLNLWGTDRAINLFWKQIPSSISELSLTYHESGIPRIPIAADLELYRAQQDSTW
                     IKTRGLIDIYYQMLKHSLGIELASEKLSPGSRFPAIIAKENAQSIAAFWNYKNSQGGI
                     NPYKGLEFKALYRLTGSNLTKHLFGATEAGAKGYLPVSNHFVGFLGVEIRNLENKKAE
                     LWQQYKMGGYGTLRGYYEDEFNSFRLGWINYELRYRLNPESRIYLFFDQGFIGKENNK
                     LKTDIFGSGFGIKINTRLGLLGLEYALGYRDKHLSDLGSGMIHLGLDLAI"
     gene            complement(7490..9289)
                     /gene="lepA"
                     /locus_tag="PLB65_06715"
     CDS             complement(7490..9289)
                     /gene="lepA"
                     /locus_tag="PLB65_06715"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOQ78255.1"
                     /translation="MKEEFIRNFCIVAHIDHGKSTLADRFLEETHVIGKGTEVSQVLD
                     SMDLEREKGITIKSHAVRMVHNYQGQDYVLNLIDTPGHVDFSYEVSRALASCEGAILL
                     VDASQGIEAQTMSNLYLALDNNLEILPVINKIDLPKADIEGTETDLCEILGCLPDDII
                     KVSAKTGEGVKALLDAVITHLPAPKGNPEATPKALIFDSYFDMYRGVVVLVRIFEGRL
                     QKGDKIKLFSTAKEYEIEEIGYLGLKFSPQNELTCGEAGYIIANIKEVADARVGDTIT
                     LAKGGCEIPLPGFREPKPMVYSSIFPINGEDYENLVESIAKLKLNDASLVYEKESSAA
                     LGYGFRCGFLGMLHLEIVKERLLREYNLPILATTPSVPFLITLKSGEELQINNPVDFP
                     DPSIIETIKEPFMETEIIVPTDYIGNVMKLANERRGIQNNIQYIDDKRVALHYDLPLI
                     EIIFDFYDKLKTVSRGYASFDYTFKDYREANVVKVDILINGEKVDAMSFICHHDKAYN
                     WGKSVTETLSEVIPKHLFKIALQAAIGGKVIARSTISPLRKDVLAKCYGGDVSRKRKL
                     LEKQKEGKKKMKEIGSVTVPQEAFLSVLRADRE"
BASE COUNT         2958 a         1796 c         1948 g         2935 t
ORIGIN      
        1 atctgtatga ggaataaatg aaaagtaact tgagttatta ccgctgcatt gaatgtggga
       61 aagaatttga tcccaacgaa ctgcattatc tttgtgatga atgtggtagg gattatctac
      121 ccggaatgcc cttaaaagga gttttggaag ctatatttaa ctatgcagaa ataggaagaa
      181 gatggaaaga gaggcccgac ccgcttttat tcagtgcagt aaatcctgaa ttttatcctc
      241 cacttccggt tgggaataca ccttttttta gaacggagat tatgggttta gaaagcgaga
      301 gaatggcgtc ctccagctac ccaattggaa ttaaatttga tggtttaaat cccagcggaa
      361 gttataaaga tcgtgccact caattggtag tagcggatgc tttgcaaaaa ggaattacag
      421 aaatcgtaac tgccagtact ggaaatgcag cttgttcact tgcttgttta ggtgcctcag
      481 ccagattaaa aattgttatt tttgctcctg agagtgctcc tccggcaaaa cttattcaaa
      541 ttcaagtgca taatgcagaa ctgcataaag ttcagggtag ctatgatgat gccttcaaag
      601 ccgctttgga atatactaaa acccataatt gtatgtgtag aaatacaggt tatcatcctt
      661 tgactataga tggcaaaaag agcgccggac tggagattta tattcagaac ggttttaagg
      721 ttccagattg gataattatt ccggtggggg atggagttat tttaactggt atctacaaag
      781 catttgtgga tttgcaaaga gccggcatta tacaaaaatt gcctcagctt ttatgtgtgc
      841 aggcagaaag cagtgatgct attacatctt attgggaaac aggaagatac aaggatgctg
      901 aaaatcctat taccattgcc gattccatca aagttaaaac accggcatta gcgcattggt
      961 cagttaaagc attgaaagaa acaagaggaa agtgtattcg ggtgagcgat tttgaaattc
     1021 aggaagcaca gttaaaactg gcagcagaaa caggtgtttt tgctgagcca tcttcggtgg
     1081 caactttggc aggactgcaa aaagcgcttg ccaaaaaatg gataaatagt aacgaggata
     1141 ttgttttact aattaccggt catggtttga aagatcctgg agccgtaaaa atatgaaagc
     1201 aaccggaata tacgattttc atgtacatat aggcgaaact attggcggac accttttagc
     1261 tgatagctgg aagagtttca accgtttata tgagcataac ggtttagaag gcattggtgt
     1321 atttgtaacc gaaagcccca atgaatccct ttctgccaaa ttatccagaa tgcgcaaagc
     1381 ttcacgcagt ttttcaggaa aggttttttg gcatctaact cccagaaacc tggatatgcg
     1441 taaacttgaa atcatcttca aaaatgatac cgattttaag ctgtatacaa cctatcgcga
     1501 aagtggatta tatcaaagct atgaaagtat tgaacatttt atgatagata tgccaaatcc
     1561 gaaaaagcgg ctattgatac actgtgagga tgatgaaatt atcagtgaat atagtgagcg
     1621 ttaccctttt cataaacctt cagaccactg tttgcggcgt ccagaaaaag cagaaattag
     1681 ggcagtggat aaagtgctta acttgtccat aaaacaccat tatcctattc atttagttca
     1741 tatttcttct ccgcaggcag cgcttttaat tcagcaggca aaaaaggaag cggattatat
     1801 tacttgtgaa acaacacctc attatctgct tttgaatgaa gaggttttga atgacctaga
     1861 aggtcatcgc tggctttgtt ctccaccttt aagaagcgaa aacagtagag gtcttttatt
     1921 ggaactttta caagatggga tttttgatat tatagccagt gaccattgtg cttttcctac
     1981 ggagacaaaa gatataggag ctttgtatcc tgcagaaact ccaatgggaa ttgccggaag
     2041 cggagttctt ttttctttgc tggccgagca actggtagca accggaaaat tgagtatgga
     2101 tcagcttttc acttatataa gcataaatcc ggctcgactg atgggtttca atatagaaga
     2161 agataaactc aattgtgaac gattgggagc tcctattcct gttgttccca gttgggcaaa
     2221 tacacctaat ccctggcttg atttttggag tcattatgaa ttgaggaggc atcataatga
     2281 agtttaataa tgcctttcgt aacgatgcca ttgccaaagt taccggcaga gcaaaatatg
     2341 ccgatgatta taagttgccc aatatgcttt atgccgtccc tttacattct caagttgcca
     2401 gtgctataat aaataacata gattctactg aagcgcttaa gcagaaaggt gtggtagctg
     2461 tttatacggc aaaagatatc cccggcaaca tcaaatatgg ccagattatt atggactgtc
     2521 ctactctcgt taataaaaga attcgatccg cgggggatgt tcttgctttg atagtggcag
     2581 aaacccgaga aatagccatt tcagctttgc cattgataaa agtggagtta tcagaattac
     2641 ccgctgtttt tgatcccgaa gaagcgataa aagagaacag tcctatagtt aatcctgatt
     2701 ttgggagcaa tatttgtaac taccataaag tgcgaactgg cgatatagat aaaggcgaaa
     2761 aagaagcaga tctcattatt gaacaggagt ttaatacttc acgcgtggaa cattcttatc
     2821 tggaaccgga aacagcactg tgttttttaa gacaggatgg cgttttagaa gtgctgggta
     2881 gtatgcaaca tcctttttct accagaaggt ttgttgcctc cactttgggc tgtaaattaa
     2941 gtgaagtgga agtaaaaaat ataccaatgg gtggaggctt tggcggcaaa gatgacacag
     3001 cagcattagt ttgtgcccgg gcagctttat gtgcgtattt aacaaaaagg cctgtgaagt
     3061 taacttatac cagggaaatg agtatgcagg aaagttataa acggcatcct tacaagctac
     3121 aatacaaaat ggggctaaaa aataacggga aaatcgtctt tatgaaagtt cggattgtag
     3181 cagacggagg tgcatattgt agtgtaactc cctgggtaac ttggcgttcc acagttcaaa
     3241 gttgtggctg ttatgaggtt cccaatgtgc agagtgatgt ttatggtgtt tataccaata
     3301 acattttttc tggtgctttc cgaggttttg gctcgccgca agtaaatttt gccatagaac
     3361 aattagttga gatggcagca gaaagattaa atatggatgc cactgaattg cgtaaaataa
     3421 atatggtgca tcaggattcc gttacgatca cccagcagaa gctaaataat catacggttt
     3481 ctttaaacca agttatggaa agcgttctga cagagattga ttatgcaaat aaagtgaaaa
     3541 aatgtagttt tggcaagcct gaaattgagg actggtatgg aatcggctat gcaatttgtt
     3601 ataggggggt tagtttagga gcagaaggaa ccgatatgaa tagtgccatc attaatattc
     3661 aacctgatgg cagcgtttta ttggaaaccg gggttcacga aaatgggcag ggagctcaaa
     3721 gtgcgatgat tttaattgcc tcggaagaac ttggcatccc taaagaactt attcgttatc
     3781 gtttaccttc cacttccaat attcccgatg gaggatctac agttgcttcc agaggaacaa
     3841 ttatgggtgg tagctcagtt attaatgctg ccaaaattat taaagagcgt ctctgtgcag
     3901 gagtggaaaa aacctataat ataacggact gtacctataa agacggttct gtatattctg
     3961 ctcagggcaa tgtaatagcc gattattttg aggcagtgcg tttatgttat gagcatcaaa
     4021 tttatcctta tgccttcggg gtattttcag ctcctccaat tagctgggat gaaaataccg
     4081 gacacggaaa tgcttatttt agttggatct acagctgtca ggcggttgaa ttgaaggtaa
     4141 acaatagaac gggtaaaatt actctgatga atctggttgc cgctcatgat attggcaaag
     4201 cgataaatcc ggctttactg gaaggtcaaa tttacggagg aatggctatg ggagcaggtt
     4261 atgctcttta cgaaaatttt cctctcatta atggcaagcc acaggttagc aattttaaca
     4321 aatattatgt cttgcgtagc acggatttgc ctgagatgac ggcaattata atagaaaatg
     4381 cagaccccaa ttccccttcc aaaacaaaag gaattggaga gccggctttg gaaattactg
     4441 ctccggcaat tgccaatgcc ctttacagag ccaccggaaa aagatttacc gatttgccta
     4501 tggcacagca agtaaaggac tatgttcaag ggaggcaaaa atgaaaatta cggttaatga
     4561 catcgaatat aatgtcccca aagaatatgc tgaaacaaaa ctttctacct atttgcggga
     4621 aaatctaaaa ttaaccggaa ccaaaatcgg ttgcgacatc ggaatgtgtg gaagttgcac
     4681 tgttttagta aattatcaac ccaggcgttc ctgcctgcta aaactaaaag atgtctgcga
     4741 taaacaaatt atcacgattg agggactggc aaatccagat ggaactttac atcccgttca
     4801 gcaggcattt attgatgctg gagctattca atgtggtttt tgcactccag gaatgatttt
     4861 aactgcctat gcctttttgc tgaaaaataa aaagcccact cgcgaagaag cgagaaatgt
     4921 aatgaaagga aatatttgca gatgcacggg ctatcaacaa attattgatg cagtgatgtt
     4981 ggctgcagaa agaatgaata aattgtaact aaagcagatt ttgcagaggg cagagagctg
     5041 agggctgagg gctgagggct gagagctgag ggctgagagc ttagggcgga gagaaaaaat
     5101 aggcctggga tgaacaggat tattgggatt ttagagattg aaatttggaa ctgaggattt
     5161 acaggatttc aaaaaaagtc ccagcgggac gacaggtaat aacgaagggt ttcaaccctt
     5221 tcaactttat aaccttttat tttacaaata acttatgcta tcttccaaaa aataacactc
     5281 gtaggatcag ctaaatagca attacatttc tttcaataga cctctaattc attttgttag
     5341 taaaaaaaac gggcatccga ggacacccgt tttttaagga ctgcgctatt gtttttttaa
     5401 gctgaattac tatttgccag ttggggtctg agttgcagga gtttcgcctt gagtaggagc
     5461 aatagtagta tcaacttgag gagttgtttc ctcaattgga gctgtctctt ctacttgagg
     5521 ttcttctgct ttctgtttgc aaccagtaac aagtgtgctg atcagcaaca tagcgagtaa
     5581 agtgattaag agaacttttt tcattgattc ctccataagt aaattcccat ttgggtactt
     5641 aggttttttt gttagttatt ggcaagacat ataaaaattt gccgattcta ttgaaggcct
     5701 gatagggtaa aaacccttgt cgatccttca cacagtcctt agacacatct atatacttgt
     5761 taatataaga cagctttttt acagttcgca ttcttggcaa gcattttttt ttagatagct
     5821 agatcaagtc ctaaatgaat catcccagaa ccaaggtctg ataagtgttt atccctatat
     5881 cccaaagcgt attctaatcc taataaacct aagcgggtat ttattttaat tccaaatccc
     5941 gacccaaaaa tatctgtttt taatttatta ttttcttttc caataaaccc ctgatcaaaa
     6001 aataagtaaa ttcgtgattc tggatttagg cgataacgca attcataatt aatccaaccc
     6061 aagcgaaagg agttaaactc atcctcgtaa taacctcgta aagttccgta accgcccatt
     6121 ttatactgtt gccatagttc tgctttctta ttttccaaat tgcgaatttc cactcctaaa
     6181 aagccaacaa aatgattgct aacaggaaga taaccttttg ctcctgcttc tgtagctccg
     6241 aataaatgct ttgttaagtt gctccctgtt agacggtata gagctttaaa ttccaaacct
     6301 ttataaggat ttatgccacc ttgagaattt ttgtaattcc aaaaggcagc aatactttgg
     6361 gcattttctt tagcgataat ggcaggaaag cgagaaccgg gacttaattt ttcgctggcg
     6421 agttcaatgc ctaagctgtg tttaagcatc tgataatata tatcaattaa tcctcgggtt
     6481 tttatccaag ttgaatcttg ttgtgctcta taaagttcta aatccgcagc gattggaatt
     6541 ctaggtatac cagattcatg ataggttaac gataattcgc taattgaaga aggaatttgt
     6601 ttccagaaaa ggtttatggc gcgatctgtt ccccaaaggt ttaaaaactg cagtcggatt
     6661 tgacctgaga gtttggttgt attattaatg cgattcattc ccaaaacgcc ttccagatag
     6721 gtcattttac cttcttcaac atttattaat aaggtattat cgtttatagg ctcaatgaag
     6781 cattggcgga tgtaactctt gcgaaggatg ttttcttgtg cttgcgttat ggattccaga
     6841 gtaatgatgt ccaaattatc taatccggaa agctgcagaa gggttttatc cttcgtaact
     6901 ttatttccgc gaaagatata attatttacg cgtaaaggtt tgccttcttc aataccaata
     6961 tatgcggtta atttatcttc cagaactaaa gaatcaagct gaactttggc aaatagatat
     7021 cctctgctat tatacagaga aagaacctgc tgcataattc ctggcagctg atttaaatgg
     7081 taattccttt cgggggagag cagcagaaat tgatataatt ttgcttcgct gaaatattgc
     7141 atacctgcaa agcggatttg catttcgtcg gaaggagctt tttcctgaag atgaaaaaga
     7201 aaggaaagag tttctccctc ttccggaata agatctggag cagagatcac gataaaatat
     7261 ctgcccatat cctgaaagta cttatagagt ttggcagttg cattattaat cagctcagga
     7321 ttgtattcct gtccggattt tatgccactg gcttttatta attctgattc ttcgattccg
     7381 gaaataccga caaaatttac ttttccaatg gttagcgcca aaagcggtga gacgaagaca
     7441 aataaaatgt agattaaata atagcgtaac ttaggtgaac agcacaccct tattccctat
     7501 ctgctctcaa aacagaaaga aaagcttctt gaggaacagt aaccgatcca atttctttca
     7561 tctttttttt accttccttc tgtttttcca atagcttgcg tttacgggaa acatctccac
     7621 cgtaacattt tgccaaaaca tccttccgta atggtgaaat agtacttctg gctattactt
     7681 tacccccgat tgccgcttgt agggcaattt tgaaaagatg cttgggaata acttcagaaa
     7741 gggtttcggt aacacttttc ccccagttat atgctttatc gtgatggcag ataaaactca
     7801 tcgcatctac cttctctcca ttaatcagga tatccacttt cacaacattg gcttctcggt
     7861 agtccttaaa agtataatca aaagaagcat aaccgcggct aactgttttc agtttatcat
     7921 aaaaatcgaa gataatctct attaaaggca gatcataatg cagagcaact cttttatcat
     7981 ctatatattg aatattattt tggatgccac gacgctcatt agccagcttc ataacattgc
     8041 ctatataatc ggttggaaca attatttccg tctccataaa gggctctttg atggtttcaa
     8101 taatgctggg atcaggaaaa tccacaggat tatttatttg aagttcttct ccacttttca
     8161 gggttattaa aaaaggaaca ctgggtgtgg tagctaatat gggaagatta tattcccgta
     8221 agagacgctc tttaacaatt tccagatgaa gcattcccaa aaatccacag cgaaaaccat
     8281 atcctaaagc tgctgaactt tccttttcat aaaccagaga cgcatcattt aattttagct
     8341 tggcgatgga ttccaccaaa ttttcataat cctctccatt aataggaaaa atactgctat
     8401 agaccatagg cttaggttct ctgaaaccag gtaaaggaat ttcgcaacca cctttggcta
     8461 aagtaattgt atccccaact cgggcatcag ctacttcttt gatgttggca ataatataac
     8521 ctgcttctcc acaggtaagt tcattttgag gcgagaattt taagcccaga taaccaattt
     8581 cctcaatttc atattctttg gcggttgaaa agagctttat tttatctccc ttttgcagac
     8641 ggccttcaaa tatacgcact aaaactacaa ctccccgata catatcaaaa taggaatcaa
     8701 aaatcaatgc tttaggggtt gcttcaggat ttcctttggg agctggtaaa tgagtaataa
     8761 ctgcatctaa aagtgctttt acaccctctc cagttttagc gcttactttt attatatcat
     8821 ctggcaaaca acctaatatt tcgcataaat cagtttctgt gccttcaata tctgccttgg
     8881 gaagatcaat cttattaata acaggcaaga tttccagatt gttatccaaa gccaaatata
     8941 aattactcat tgtttgtgct tcaattcctt gagaggcatc cactaataaa atagcccctt
     9001 cacaagaagc caaagcacgt gaaacctcat aggaaaaatc cacatgacca ggggtatcaa
     9061 tcagattcaa aacataatct tgaccttggt agttatgaac catacgcaca gcatgacttt
     9121 taatagtaat tcccttctcg cgttccagat ccatactatc taaaacttgt gagacctctg
     9181 ttcctttgcc aattacatgt gtttcttcca gaaatctatc tgctaaagtg gatttaccat
     9241 gatctatatg agccacaatg cagaaattgc ggataaactc ctctttcatc taagttcctt
     9301 aatttgtcgt tgttatctta tagttccctt tggggaaatt ataatacatt aattagtaag
     9361 atccattgcc gtacttagtt aacttcctgc ctgggcagaa ataacaagcg gctttttaaa
     9421 aagaaataat tttagtccat ttatgtatac tatccctttt tgtcaatcac tttgggaaaa
     9481 gctcttccct cgcctgcttt ttattatcgt tttgactcta aaccgatcct tttttgtgct
     9541 gaaacgagat tggtaaggca acaatatatt ttgtcccccg ccttttcctg acaaagtgga
     9601 ctcgtttcgt tttgactcta aaccgattct tttttgt
//
LOCUS       DAOSTH010000062         9373 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_7063_length_9373_cov_10.632432, whole genome shotgun sequence.
ACCESSION   DAOSTH010000062 DAOSTH010000000
VERSION     DAOSTH010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 9373)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9373)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9373
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7063_length_9373_cov_10.632432"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1146..1502
                     /locus_tag="PLB65_06720"
     CDS             1146..1502
                     /locus_tag="PLB65_06720"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HOQ78256.1"
                     /translation="MLRQILLSKLHRATVTECDINYNGSIKIDAELLEKSGMREFEKV
                     EIFNITNGNRFSTYIIVGERGSGIISINGAAARLVQVGDKVIILNYGIIEEDKMEDYK
                     PRIVVLNDENRIEQIH"
     gene            1502..2344
                     /gene="panC"
                     /locus_tag="PLB65_06725"
     CDS             1502..2344
                     /gene="panC"
                     /locus_tag="PLB65_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOQ78257.1"
                     /translation="MQIITDIKQMQEIGNNWSNSQTIGFVPTMGYFHKGHLSLVAEAN
                     KNCDITVVSIFVNPSQFGPNEDFSSYPRDLQRDLELLNQYKVDYVFNPTPDQMYPAGY
                     KTWINVEELSSILCGASRPGHFRGVATVITKLINIIKPSLMFMGEKDFQQIVVLQTML
                     KDLNFHTQIHPCPIIREADGLAMSSRNVYLNSEQRQQALCLSLAIKQAQEQYKQGIDD
                     PQILKKNASDLIIRAGGRIDYIKFVDSVSLQEVKQAKDDTRILLAVFIGKTRLIDNAA
                     LGSY"
     gene            2518..3165
                     /locus_tag="PLB65_06730"
     CDS             2518..3165
                     /locus_tag="PLB65_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78258.1"
                     /translation="MRKFNLLIPLAIILLLLASCATNEQGQSTKLVTKYDIALTKVSR
                     GNEVNIRSKPIIADTTIVNKTRYRYEDELMNTIWYASENGFELTLYNPSDKNLTLDWD
                     KGLYLDYDNIGHRLLISSTPDSEKDKPQAPSTIAPRNNLTETIFSADHTKPSTLLGVY
                     VRTPLLPTEYAKAVRYHGKEMKIKLPIIVDNTTYNYEFTFKIVSVRQAPSSSSLF"
     gene            complement(3663..8876)
                     /locus_tag="PLB65_06735"
     CDS             complement(3663..8876)
                     /locus_tag="PLB65_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOQ78259.1"
                     /translation="MAKRTLLVMLLIIAAVLVSAQTIPYPVDSKEYQEYKEGLIGKMP
                     VQPPVYSNVPKGKQVPLVYQKQQRDGLLIPLDDSFQLAMEGNDDYYTSVIPLPFAFDF
                     YGTTYTEFYINNNGNVSFGNPYSTFTPSGFPVNDFPMLAPFWADIDTRSVGQVFYKME
                     SSRVTVIWNAVGYFSNHTDKLNTFELIFTNGWDPVVGAGYNVAFSYGDMQWTTGDASG
                     GENGFGGVPATVGLNKGDGIYYGLIGRFDHEGFDYNGPGGAPSGVSYLDNQLFRFTAI
                     QGNFLPPVFGNLPVETVNLQEGESIQLHINASSPAPGNTVYAEIQHDFTVGFNAEIIP
                     GNPCYINLTINGLRNNVGLHNITITATDNGNPSLSTVAGFTMRIIPHLGDFLIVTNFY
                     PPSINIVDVATNEVYGPFLEGQLGDYYLVDVVVSFDAQFALISNFNNATVYQIDLSNL
                     LEPTIIASYNLSFSAEDITLSADNRYAIVADGGPSNTYIAVLDLQSRTTVQVLDIYPH
                     NAQGVTIGPGGKVLINDCTNGYVHQYLLNFATGNLSYSNVSVPVANCMNTVINPGGDF
                     AIVCSLDGQSEVLQLNTDNSITVLQNLGVSSQSAVYSTDGQIALIGEYGTDNHKLSAY
                     NVQANGTLAWQQSFDLPFYEWWGYYGVDVLALSTDNSQAYITDFTGSVNNMLVNINLV
                     NGTQQNLTLPYPTGLAIGTMFLTAYFTATITNVQNCTQVQFHQHCSGNPDSYLWNFGD
                     GQSSTEPNPYHIYYTPGLYTVTLTIYKNGESDSYFQTIEAIFNTQIHLTLAGSPYYYE
                     STLDISEGSSLIIDNGVIVNFGPEAGLEVWGSIFANGATFSGSETEGWKGIILHSPVS
                     PVTFNNCNIYNAVVGLKIINSSFTISNLMISKTQIFTGEIGLKVQDASNMVFNNLQVI
                     GYTTGIVFENEVRTTSSPILNNTRIRNSSSSIRTESKGLYVFGAIGLTLDNASIEEYD
                     YGVYWDGLGTTDYRSVPILNNTRIRNSSSSLRNISSGIKMIDISRVIMSNDSIGGYPT
                     GLEIINNESTLLTTNATLKNTRIRNSSSSLRTETKGAYLNGHIAATIDSLEIEDYSIG
                     IYYESDGTPFNRTIPVLSNTRIRNSSSSLREPLKGLILKNLGEVIISNNIIYPNIQNL
                     RQNTEGIGISLDNSQNAQIVYNTIWGLNNGLYASGSSNGIFNQNVIWTNGEQLNNPVI
                     LEDSNIPISNCDISYAQGVFPGTNNLNEDPLFVNPEEGNFYLKPRSPLRDTNIGALPF
                     NFDVLAAVHTYNMHPGWNLMGVPYLTRPLENTPVSIFADDLSPFYVAPNYTSIVQLNP
                     SATSDSLGHLDMTYTGSYTIPAQVHPGIGYWVRNPYATLATIDVYGLMDDGSYNVVLD
                     GTGNPNNGWYLLANPYDRLISLTDGSIILGAGMLNGMQTYDYNLHNYILIDADNPGFV
                     EPWGGFFVKAIAPDAHLYFNYPSQPRVQSEILQPLISNSNVVPCPKLKWELSLTANSG
                     EKGDIITFGVAEGAQDIYDSLDIPEIPNPPFPQKGMLQFSICNNDWQNNPGLYTRDIK
                     SSRNGSWNWNLLLNLEGLLEDGILNDIIKISAIGTDKIPANYIVRMTNPADGSSIDLK
                     NDVYLLEVDINHSEEEPWLIPLSVEVIPCSNNVDEKEHFSATNYPNPFNPETTICYNL
                     PTESFVSLEIYNLKGQLVKKLINETQSSGKHSTVWNGCDQQGHEVASGFYFYRLSTKD
                     NKLTRKILLMK"
     gene            complement(8878..>9373)
                     /locus_tag="PLB65_06740"
     CDS             complement(8878..>9373)
                     /locus_tag="PLB65_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78260.1"
                     /translation="GISPDSVIYQGFVTQMVNVTEHLYSFAPMLEPLTQYYWRIKAVS
                     STEEIWSGMWSFTTGNYEAGNMSMLEPISVTVAINALQNQVRLSWQRRRNDESYYVYW
                     SENPYAEFPEGWNGPVNVTETNYTDTLTDKCFYRVFAMPSIRGKTENKQNTKPRAIMP
                     GRGE"
BASE COUNT         3040 a         1709 c         1670 g         2954 t
ORIGIN      
        1 gcctctacaa catattataa cgcaaatcgt taaatataca aattattttt ttgaaatcgc
       61 taaaaaatat actgatttag gcaagatctt gaagctaatt gagaaaaata cagcatattt
      121 tagtgtaaac tgacaccaga acttcatgcg ttggcacaaa gaaaatgtag catgagagct
      181 cttgatgggt gttttgatag ttcgatattt catcatagtt ctaaaaatta gtgagctttt
      241 ccgtggaagt tattaaggta ccattaatca tccgttctta aaaatgaata gagtatcttc
      301 tagcaattgc caataaactt tcctgcaagt tgaataaatg atctttttcg gtttttttct
      361 gtaatttcag ataatataaa agacatactt aagtatacca ttttatatgc tttactccga
      421 ttatatgtca gccttggatc caaaagtgga ttttatgagg agattacttt actcataatc
      481 aatcaggtat atttatatct ctttatttag atactgatag ccaaaaacta tcgttatcaa
      541 gccgtcttcc ccagcccttt taactgaatt gcagagtaaa tcttattcca aattttgggc
      601 tttgggaata gtttttaggt tcacttgttc cggataattt tagatcagat tattccctta
      661 ctagaaagtg tctatcactt cttcatcatg ccttaatcac tccttaatta ttaacgcttg
      721 attaaggtta gttaaacgca taatatactt atatggaaaa acggtaaaga ggattttctc
      781 ttgtcgataa aactatagtt taatgtgttc tgtaaccctg ataagtccac aagttaaata
      841 gaaaacaaga atatgatgaa taggataatg ggaatgcgct taattgttaa tccggtaagc
      901 tccttgccca taacaaatgg ataaatcaga gattttttgg gaggcaaaga aagcaacata
      961 cagatgaatt actaagttct tacgaatatt tgttcagtat aggctaaata atcgagataa
     1021 tctccttact tctcattttt cattggtcat atatagtaac aatatttgga gagagtggaa
     1081 attttattga caaaaggcaa gcttaggaga attgggatat aaaaatacta attgggagac
     1141 cacaaatgtt aaggcaaatt cttttatcaa agcttcatag ggctacggta acggagtgtg
     1201 atataaatta taacggtagc atcaaaatag acgcagagct tctggaaaag agtggtatgc
     1261 gcgaatttga aaaggttgaa atatttaata ttacaaatgg taaccgtttt tcaacttata
     1321 taatagttgg agaacgagga agcgggatta tcagtattaa tggagcagca gcgcgtttag
     1381 ttcaagttgg tgataaagtt atcattttga attatggcat tatagaagaa gacaaaatgg
     1441 aagattataa gccccgtata gttgttctca atgatgaaaa tagaatagag caaattcact
     1501 gatgcagatt ataaccgata ttaaacagat gcaggagatc ggtaacaact ggtcaaatag
     1561 tcaaacaata ggttttgtgc ctacaatggg ttattttcac aaggggcatc tttctctggt
     1621 agctgaagca aataagaatt gtgatataac ggttgtttcc atttttgtaa atccttcaca
     1681 gtttggtcca aatgaggatt ttagtagtta cccgcgtgat ttacaacggg atttggaact
     1741 cttgaatcaa tataaagtgg attatgtatt taatcctact cccgaccaaa tgtatccagc
     1801 aggttataaa acttggataa atgtagagga gctcagttct attctttgtg gagcaagtcg
     1861 tccaggtcat tttagagggg ttgcaacagt aattacaaag cttataaaca taataaagcc
     1921 aagcttgatg tttatgggcg agaaggattt tcagcagatt gtggttttac aaacaatgtt
     1981 gaaggatctt aattttcaca cacagataca tccttgtccg attatcagag aagcagatgg
     2041 tttagctatg agtagccgaa atgtatatct aaactctgaa caaagacagc aagcattatg
     2101 tctttcgctg gctatcaaac aagcccaaga gcaatacaaa caaggtatag atgatcccca
     2161 aattctgaaa aagaacgcct ctgatttaat aattagagct ggtggtagga ttgattatat
     2221 taaatttgta gattctgttt ctttgcaaga agttaagcaa gccaaagatg atacccgcat
     2281 tcttttagca gtttttattg gcaaaacccg tcttatagat aatgctgctt taggaagtta
     2341 ctaactttat acagaatcag acagttaaag caattgattg atttcaggca ttttttgcta
     2401 aggataatag tatagataca aaatatatcg tgtaggaagc aaatatcaag cttgacaaaa
     2461 taaacgatat agcaaatatg aaatttacta ttaataacgc caagggagtt aaaaaaaatg
     2521 agaaaattta atttgcttat cccacttgcc attattttgt tgttgcttgc atcttgcgcc
     2581 acaaacgaac aagggcaatc aaccaagctt gttactaaat acgatattgc tcttacaaaa
     2641 gtatcgagag ggaatgaagt aaatatccgt agcaaaccta ttattgctga tactactatt
     2701 gtaaataaaa ctcgctaccg ctatgaagat gaattaatga atactatatg gtatgcttca
     2761 gaaaacggct ttgagttaac tctttataat ccttcggata aaaatctaac attggactgg
     2821 gacaaaggac tatatttgga ttatgataat atcggtcatc gtttattaat ttccagcaca
     2881 cctgattctg aaaaagataa acctcaggca ccaagtacca tcgctccgcg taataatctc
     2941 acagaaacta ttttttcagc agatcatact aagccgtcaa ctcttttagg agtatatgtt
     3001 agaacacctc tcttacctac agaatacgca aaagctgtgc gctatcatgg taaggagatg
     3061 aaaattaagc ttccgataat tgtagataac actacctaca attatgaatt tactttcaaa
     3121 attgtaagtg tgagacaggc gccatccagt agttcacttt tctaactgct aattttacgg
     3181 aggattatat tcctccgttt tttttacctt gcatagcact attgaaatta aagggaacat
     3241 ttgtaatcta ttggttttct catttttctg tattctatat tttgctatgg agttatgaag
     3301 agagagatat agctatctca tcgtaacata ttacctctcg aagattcata atcgataacc
     3361 tgttgagact atattacttc gagaagtttc gtattgggca atacttttaa tcattatttt
     3421 cgtgtgaata tagcaagcgt cgttataact acaattagcc agtatatttt actttatgca
     3481 agcaagatag tatttttctc tttaagaatg aaagcaacac tatatcctaa gagacatcat
     3541 cccttgtgat atcttggaag cagtatttat cactctgaca aagagacagg taagtaaatt
     3601 actccatttt tgtctttagc tggctacatt tacttgtttc ttttttatca ttcctcacta
     3661 tatcatttca ttaatagtat ttttctagtt agtttattat ccttcgtgct caggcgatag
     3721 aaataaaatc cggaagcaac ttcgtgacct tgttgatcac atccattcca aacggttgaa
     3781 tgttttccag aggattgtgt ttcatttatc aattttttaa ccaattgtcc tttcaaatta
     3841 tatatttcca gagagacgaa gctctcggta ggcaaattat aacagattgt agtttccgga
     3901 ttgaatggat tggggtaatt tgtggcagag aaatgttctt tttcatcaac attattggag
     3961 caaggtatca cttccacaga taaaggaatc agccagggct cttcttctga gtgattaata
     4021 tctacttcaa gtaagtaaac atcatttttt aaatcgatac tactgccatc ggcaggattt
     4081 gtcattcgga caatataatt agcaggtatc ttatctgtac ctatggcact gattttaatg
     4141 atatcattta aaataccatc ttctaatagt ccttctagat tgaggagtaa attccaattc
     4201 caggaaccat ttctactact tttaatatcc cttgtataaa gtccggggtt attttgccaa
     4261 tcgttattgc agatactaaa ttgaagcata cctttttgtg gaaaaggagg attgggaatt
     4321 tctggaatat ctaatgaatc ataaatatcc tgtgctcctt cagcaacccc aaaagttatt
     4381 atatcacctt tctctcctga attagctgta agcgataatt cccattttag tttaggacag
     4441 ggaactacat tggaattact aattaatggt tgaagtattt cactttgtac acgaggttgt
     4501 gaaggataat tgaaatataa atgtgcgtcg ggagctatag cttttacaaa aaagccaccc
     4561 cagggttcta caaaaccggg attatcggca tcaattaaga tataattatg caggttgtaa
     4621 tcgtaagttt gcataccgtt taacatccct gctcctaaaa ttatgctgcc gtccgttaaa
     4681 gaaataagac ggtcataagg atttgccaaa agataccagc cattattggg atttccggtt
     4741 ccatctaaaa caacattata actgccatca tccatcaaac cgtaaacatc tatggttgct
     4801 aatgtagcat aaggattgcg tacccaataa ccaattccgg gatgaacttg agctggaata
     4861 gtataacttc ctgtataagt catatcaaga tgacctaggg aatcacttgt ggcagatgga
     4921 tttaattgca caatggaagt ataattcgga gccacataaa aaggagaaag atcatcggca
     4981 aaaatactta ccggggtgtt ctccaagggt cgggttaaat atggcacacc cataagattc
     5041 caaccaggat gcatattata agtatgtact gcagctaaaa catcaaaatt aaaaggcagc
     5101 gcaccaatat ttgtatctct aagcggagaa cgcggtttca aataaaaatt accttcctcc
     5161 ggattaacaa agagaggatc ttcattcaaa ttatttgttc cagggaaaac accttgagca
     5221 taagaaatat cacagttact aattggaata ttgctgtctt ccaagataac aggattattt
     5281 aattgttctc cattagtcca gataacattt tgattaaaga taccgttact actacccgaa
     5341 gcatacaaac cattattcaa accccaaatc gtgttataaa ctatttgtgc attttgactg
     5401 ttatctaagg atattcctat tccttcagta ttttgtctca agttttggat gttcggataa
     5461 attatattgt tacttataat aacttcgcct agatttttta ggatcaatcc ctttaacggt
     5521 tcccgtaacg aactgctgct attacgaatt cttgtattag agagtacagg tattgtccgg
     5581 ttaaagggag ttccatcact ttcataatat attccaatag aataatcttc tatctccagc
     5641 gaatctatag tagcagcaat atgaccattt aagtatgccc ccttagtttc tgttcgtaag
     5701 gaactactac tatttcggat acgggtgttt ttaagggtgg cattagttgt taacaaagta
     5761 gattcattat tgatgatttc caaaccggtc ggataaccac caatagaatc attggacatt
     5821 attactcttg agatatcaat catttttata ccactggaaa tattgcgtag ggagctactg
     5881 ctgtttctaa tgcgggtgtt gttgagaatt ggaacactac gataatcagt tgttcctaaa
     5941 ccatcccaat aaacaccata atcatattct tcaatactgg cattatctaa agttagccct
     6001 attgcaccaa atacatataa tcctttactt tctgtgcgaa tagaactgct actgttgcga
     6061 atacgagtat tattaagaat agggctactg gttgttctaa cttcattctc aaaaacaatt
     6121 ccagttgtgt aaccaataac ctgtagatta ttgaagacca tattagaggc atcttgcact
     6181 ttcagtccaa tttctccagt aaatatttga gttttgctaa tcatcaaatt tgatatcgta
     6241 aaactgctgt taataatctt taaacccacc acggcattat agatattgca attgttgaaa
     6301 gttacggggg aaacaggaga atgcaaaata attcccttcc agccctctgt ttcacttccg
     6361 gaaaaagtag ctccattagc aaaaattgaa ccccagacct ctaaacctgc ttccggacca
     6421 aagtttacta tcacaccatt atcaataatc agagagctcc cttccgaaat atcaagcgta
     6481 gattcataat aatagggtga ccctgctaaa gtaagatgaa tttgagtatt gaaaattgct
     6541 tctatagttt gaaaatagct gtcactttcc ccatttttat agatagttag cgttactgta
     6601 tataaaccgg gagtgtaata tatatgataa ggattgggtt cagtgctgct ttgtccatct
     6661 ccaaagttcc acaaatagga atcaggatta ccgctgcagt gttgatgaaa ttgaacctgg
     6721 gtacaatttt ggacattggt tatggttgct gtaaaatatg ccgttaagaa cattgttcca
     6781 atggctaaac cagtaggata gggcaaagtt aaattctgtt gtgtgccatt taccagattt
     6841 atgttaacta gcatattgtt gactgatcca gtaaaatcag taatatatgc ctggctgtta
     6901 tcagtgctta aagctaaaac atccactcca taatatcccc accattcata aaaaggtaaa
     6961 tcaaaactct gctgccaagc taaagtccca tttgcttgaa cattataggc agaaagctta
     7021 tgattatctg ttccatattc accaattaag gcaatttgcc cgtctgttga atatacagct
     7081 gattgagaac taacacccag gttttgtaac acagttattg aattatcagt attcagctgt
     7141 agaacttctg attgtccatc taaagaacag acaatagcaa aatccccacc gggattgata
     7201 actgtattca tacaattagc tacgggaaca gaaacattgg aataggataa attaccggtg
     7261 gcaaaattta aaagatactg atgcacataa ccattagtgc aatcatttat cagcaccttg
     7321 ccaccaggac ctatagttac tccttgtgca ttatgaggat aaatatccaa aacctgcact
     7381 gtggttctgg actgcaaatc caatactgca atataagtgt ttgaaggacc tccatcagct
     7441 acaatagcat aacggttatc tgcactcaaa gtaatatcct cagcgctaaa actcaaatta
     7501 taagaagcaa taattgtcgg ttccaataag ttgctgaggt ctatttgata gacggtagca
     7561 ttgttaaaat tggagatcag ggcaaattgg gcatcaaaag agacaactac atctacaaga
     7621 taataatcac ccaattgacc ttccagaaaa ggaccataga cctcattggt agctacatcc
     7681 acaatattga tactgggggg atagaaattg gtaactatta aaaaatctcc caggtgagga
     7741 ataatcctca ttgtaaagcc ggcaactgtg gaaagagaag gatttccatt atctgtagcg
     7801 gtaatagtta tattatgcaa tcctacatta ttgcgtaaac cgttaatcgt caaattgata
     7861 taacagggat ttccgggtat aatttctgca ttgaaaccga ctgtaaaatc gtgctgaatt
     7921 tctgcgtaaa cagtattccc cggggcaggc gaagaagcat ttatatgtaa ctgaatgctt
     7981 tccccctctt gcaggttaac tgtttctact ggaagattac caaaaacagg aggcaggaaa
     8041 tttccctgaa tggcagtaaa cctgaataac tgattatcca gataactaac tccggaggga
     8101 gcacctcctg gtccattata gtcaaatcct tcgtgatcaa atctgccaat taatccatag
     8161 taaattccat cccctttgtt taaaccaacc gttgccggaa cacctccaaa accattttct
     8221 cctccggaag catctccagt tgtccattgc atatctccat aactgaaagc tacattataa
     8281 ccggctccca caacagggtc ccaaccatta gtaaaaataa gttcaaaggt attaagttta
     8341 tcggtatgat tactaaaata gccaactgca ttccaaatta ccgttactct actggattcc
     8401 attttgtaaa aaacctgacc cactgacctg gtatctatat ctgcccaaaa gggagctaac
     8461 ataggaaaat catttaccgg aaatccagag ggagtgaatg tgctgtaagg attgccaaag
     8521 gaaacattgc cgttgttgtt gatgtaaaat tccgtgtagg tagttccata aaaatcaaaa
     8581 gcaaacggta atggaatcac ggaagtgtaa taatcatcgt ttccttccat tgccagctgg
     8641 aaactatcat ccagcggaat taaaagtcca tcccgctgct gtttttgata cactaaggga
     8701 acttgtttcc ctttaggcac attggaataa acaggtggct gaacaggcat tttgcctatc
     8761 agaccttctt tgtattcctg atattcttta gaatctacag gatagggaat tgtttgggcg
     8821 ctaactaata ctgctgctat tatcaacagc attaccaata atgttctttt tgccatttta
     8881 ttctcctctt ccaggcatta ttgccctcgg tttcgtattt tgtttatttt ctgtttttcc
     8941 ccggatagat ggcattgcaa atacccggta gaaacatttg tctgttaaag tatccgtata
     9001 gttggtctct gttacattta caggaccgtt ccaaccctca ggaaactccg cataaggatt
     9061 ttccgaccaa tatacatagt agctttcatc attccttctg cgttgccagg ataaacgcac
     9121 ctgattttgt aaggcattaa tcgctacggt aacagaaatc ggctccaaca tagacatatt
     9181 tcctgcttca taattgccag tggtgaaact ccacattcca ctccagattt cttcggttga
     9241 agagacagct tttatccgcc aatagtattg tgttaaaggt tccagcattg gagcgaagga
     9301 ataaagatgt tctgtaacat ttaccatttg agttacaaag ccctgataga taactgaatc
     9361 aggactaata ccc
//
LOCUS       DAOSTH010000063         8927 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_7449_length_8927_cov_13.995153, whole genome shotgun sequence.
ACCESSION   DAOSTH010000063 DAOSTH010000000
VERSION     DAOSTH010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8927
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7449_length_8927_cov_13.995153"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            81..863
                     /gene="tpiA"
                     /locus_tag="PLB65_06745"
     CDS             81..863
                     /gene="tpiA"
                     /locus_tag="PLB65_06745"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOQ78261.1"
                     /translation="MRKLYIAGNWKMNKGLMETRVFVQRVVPEINKQDLGNVIPLIAP
                     AYPFLEEALRESFSSALQVAAQDVSYHSEGAYTGEVSAQMLASMELKYCIVGHSERRQ
                     YHNETDAMVKARMLKLFEHNITPIVCIGETLAQREEGKTEEVIINQLNGCLEGIKLIK
                     GTEIIIAYEPVWAIGTGKTATPWQAQEVHKLIRNWLKNNYGETVANELSILYGGSIKP
                     ANLKELLEGEDIDGGLIGGASLKEEDFLEMVKIAVASKGEKC"
     gene            857..2116
                     /gene="serS"
                     /locus_tag="PLB65_06750"
     CDS             857..2116
                     /gene="serS"
                     /locus_tag="PLB65_06750"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOQ78262.1"
                     /translation="MLDLKFIRNNVELVRQAIINKNEKADLDALLSIDDQRRKLQFEF
                     DNLKAHQNSVSAIIAQRKKAGEKIDNELTEMSKVAEEIKGLANQLGATNAELEKLLLT
                     IPNIPQADVPVGKDETANKVIKVWGEPKQFSFTPKDHLELAIQNNLLDLPRGAKISGS
                     GFPVYTGEGARLERYLINFMLEYHCQKHNYTELMVPLAVNRKTMTGTGQLPKLEEDMY
                     QIEKDDLFLIPTAEVPVTNIYTDEILIWKDLPKRYVAYTPCFRREAGSYGKETRGLQR
                     LHQFNKVELVQFTEPEKSAEALEEILANAEAILQAFNLHYRVVTLCTGDLSFASQKTY
                     DLEVWAPGTGKYLEVSSVSNFGEFQARRANIRFKDKTGKVRHLHTLNGSGVATPRLLI
                     AILETYQQEDGTIKLPSVLDPFLRLTL"
     gene            2139..3326
                     /locus_tag="PLB65_06755"
     CDS             2139..3326
                     /locus_tag="PLB65_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HOQ78263.1"
                     /translation="MNKNELGLLDIILVIAKRKYLVITICCIVAVAAIIYSLVVPEYW
                     ESKATLMPIAESGSIGSLGGSFMDILGGGLLQTDKYDMAVDFIYIMQSRKFREEVVRK
                     FNLIQYYKIKEPDSLKAMELAVRKLSEETMEMFYDQKTYLVNIKAETKDRELSRQMVQ
                     HHLDALSKYILNSKMSKGRQKRQFLEKQVEEHKQIMDSLAMRMRDFQKKNRSIDITQQ
                     TQSLIKLYSDVVAEYMQAELEYSLALTQYDEKSPIVKSLTEKMKLLKKKVQSLEKNNS
                     DLVPEYIVQIDKIPDISMQYAQMMLNVEIEKQIIEYLYPQYELAKLEEVKDLPTFEIY
                     DAPQIAGIRSKPKRAITVIVSTVAAFIFSCILALILESFQGENREKILAIKAALFRKR
                     KQS"
     gene            3716..4597
                     /locus_tag="PLB65_06760"
     CDS             3716..4597
                     /locus_tag="PLB65_06760"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOQ78264.1"
                     /translation="MLAYLGIAFWEISTWKHLPQSIYYNRAIFVPTGPFYGPNQFGAV
                     FCLLFPFYLFIPELTKNRRYLRVFSFICALLVIAIVIVEGARIAIIATLGMLLYTLFF
                     LYSKKMRRTGLLLIGLIILGLIIFAKPLVNFTISKLSAEISSIGQESKSCVMGSIKIR
                     TRLIPENLEILAQSGFMGVGAGNVERYMLSGRIYRTGGISNPHNFFLELMANYGLVFL
                     LGFLYIYGIWLYRLWRAIKANPEQKKYYEMFFFVLLMFIPAASLPSSIIWEHHYWILF
                     AFINEISHPANWMQKAI"
     gene            4594..5361
                     /gene="mazG"
                     /locus_tag="PLB65_06765"
     CDS             4594..5361
                     /gene="mazG"
                     /locus_tag="PLB65_06765"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HOQ78265.1"
                     /translation="MSKFEKLVEIVATLRTPGTGCPWDLKQTRETLVPNIIEELYEVI
                     EAIENKDDNSLKEELGDLLLHIVFQAQISKEQGLWSIDDVIDEIINKLIRRHPHVFGD
                     LNIEDADAVKVNWERLKKKEKTMRKSVLDGIPKKMPALIQAQRTQEKAAAVGFDWQDI
                     KPIMEKIDEEKEEFIEALASNEEEKIREELGDMIFTLVNLARKLHIDAEAALKECTRK
                     FVRRFNSIEEYYRKEGKDINEADLEELDSHWQRIKGH"
     gene            5306..6859
                     /locus_tag="PLB65_06770"
     CDS             5306..6859
                     /locus_tag="PLB65_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOQ78266.1"
                     /translation="MKPILKSSILTGKESRDTNLFNRLRFILIFGSLAIFIFFGIYIQ
                     FLIKQAKQEQEYIPRIFAQYIAYTDKYLREAELYAQLITEISSKYLQFTTSSDFKQDL
                     WDYISTEFMQDNPVPVIITDQNFQPQLWKNVGVPTDTLFHELSAANQRKLENLMKTMI
                     QTPLTDNGKISGYAFYSKPISFAEFIKNVDCAIIVTDHYKQPLFWRNVGIPEAANYYE
                     ISTEEQQKLAHQIQEMEEIPLSNQADSLGYIYFSNPKSLSYIRYIVILELVFALTVVF
                     FGVYGLFLLSRTEKDTLWIGLAKETAHQFGTPITSLMGWLDYLKEPPVDGSERDINQI
                     VQYMTADLEHLKTIASRFGKVGSVTKLERNNLHQVIAETVEYFRARMPHLGNKIDIHY
                     ISKIDDETVLMDKELIKWTLENLIKNCMDAMITKGGNIIITATKKDPWIYIHIRDEGK
                     GIPRRQWKRIFEPGVTSKTRGWGLGLSLVKRIIEDYHNGSIKVLQSTIGEGTTFEIKL
                     PVQGINKEK"
     gene            6861..8402
                     /locus_tag="PLB65_06775"
     CDS             6861..8402
                     /locus_tag="PLB65_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOQ78267.1"
                     /translation="MSYIFTPSQMKTLDENTINCFGLPARILMENAGKGCADYLLQNC
                     SEHLNNVIIILHGTGNNSGDGFVLARWLTIYGRRVLLCKVQDGTMTEECRLNYELCQS
                     LNIPMIGLDNISDTLEILDNSSYIGMIVDAVFGTGYKGKLPTSLKKIFQSANNSPAFK
                     VAIDIPSGLNGATGEGEDAFEADLTLCLHSPKIGSLLHTGKLKSGDLVTIPIGIPDSY
                     NDAFFSPALHIDAETFQVPKRYLLANKSDYGRVFIIGGSMGLLGSVAMSAKAALRAGA
                     GLVYLVSREEYAYFYNTNPSEVLFVGIPSKPESLLPDLSEFSSLIKKADSIVIGPGLG
                     TDEYGYIMLEFILRTSTVPTVVDADALRLIAKEPSLQQYIKKPNILLTPHFGEFCDLA
                     KIEMSALYKDTISELTKYVKNTEAKVLLKSDTTIFYDGKRMLINTTGNDGLATGGSGD
                     VLTGIIGSFCGQDMELGEAAINASFFMGFTTEYLATKRYPCSILPTDIIDNLFVRLVQ
                     EGSNE"
     gene            8395..>8927
                     /locus_tag="PLB65_06780"
     CDS             8395..>8927
                     /locus_tag="PLB65_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOQ78268.1"
                     /translation="MNKLAALNTPIFWAEGHPGSLNRETWKIEVCGSCNKPQIFSWSD
                     LMELPQRDVKGRLTSVTRWSVEGIWKGIPLSEILKTVQSNDSCKYLRFWSFGGIYDTS
                     IPIDIAFKEKSLLAHSFEGELLTEDYGGPIRAFIPYLWGYKSAKSVVKIELMDYYVSG
                     FWENRGYTDSGEIEAGP"
BASE COUNT         2933 a         1451 c         1882 g         2661 t
ORIGIN      
        1 caaaaaggat aaaaaaactt gacctaatag ccaaagctaa actttatgat ttttaagata
       61 agcttaagtg aggtatataa atgcggaagt tatacattgc cggtaactgg aagatgaata
      121 agggtttaat ggaaacgcgg gtatttgtac aaagggtagt tccagagata aacaagcagg
      181 atttggggaa tgtaattcct ttaattgcac cggcttatcc atttttggaa gaggccttaa
      241 gagagagttt tagttctgct ttacaagtgg ctgctcagga tgtatcttat cattctgagg
      301 gtgcttacac gggagaggtg tctgctcaaa tgctagcatc aatggaactt aaatattgta
      361 ttgtgggtca ttctgaacgc cgtcaatatc ataatgaaac agatgctatg gtaaaagcca
      421 gaatgttgaa gctttttgag cataatatta ctcccattgt ttgtattgga gaaacattgg
      481 ctcaaaggga agaaggcaaa acagaggaag tgattatcaa tcagcttaat ggttgtttag
      541 aaggcataaa actgataaag ggaacagaga ttattattgc ttatgaaccg gtttgggcaa
      601 ttggaacagg aaaaactgct acaccttggc aagcgcagga agtgcacaag ttaattagaa
      661 attggctgaa aaataactat ggagaaaccg ttgcgaatga actatccatt ctctatgggg
      721 gcagtataaa accggcaaat ttaaaagagc tattggaagg cgaagatatt gatggtggct
      781 taattggagg cgcttcgcta aaagaagagg actttctgga aatggtgaag atagcggtgg
      841 caagtaaagg agaaaaatgt tagacttaaa atttattcgt aataatgtgg aacttgttcg
      901 tcaagcgata atcaataaaa atgaaaaagc ggatttggat gcgctattaa gcattgatga
      961 tcaaagacgc aaactgcaat ttgagtttga taatctgaaa gcgcatcaaa acagtgtttc
     1021 agctatcatc gcgcaaagga aaaaagcggg tgaaaagata gataacgaac ttactgaaat
     1081 gagcaaagtg gctgaagaga ttaaaggact tgctaatcag ttaggcgcaa caaatgcaga
     1141 gttggaaaaa ctacttttaa ctattcccaa tattccgcaa gcggatgtac cagtgggaaa
     1201 agatgaaaca gctaataaag taataaaggt atggggtgaa cccaaacaat tttcctttac
     1261 tcccaaagat catttggaat tggctattca aaataacctt ctggatttac ctcgtggggc
     1321 aaaaattagc ggtagtggtt ttcctgttta cactggagag ggagcgcgtt tagaacgcta
     1381 tttgataaat tttatgttgg aatatcattg tcagaaacat aattacacgg aattgatggt
     1441 gcctttggct gtaaaccgaa aaacgatgac aggcacaggg caacttccta aattggaaga
     1501 agatatgtat caaatagaaa aagacgattt attcttaatt cctacagctg aagttcccgt
     1561 aactaatatt tatactgatg aaatccttat ctggaaagat ctgccgaaac gctatgttgc
     1621 ctacacaccc tgttttagaa gagaggcagg ttcctatgga aaagaaacta gaggtctgca
     1681 acgcttgcat caatttaata aagtggagtt agttcagttt actgagccgg aaaaatcagc
     1741 ggaagcatta gaagaaatct tagccaatgc ggaagcgata ttacaggcat tcaatttgca
     1801 ttatagagta gtaactcttt gcacaggcga tctatctttc gcatctcaaa aaacctatga
     1861 tttggaagtt tgggcaccag gaacaggtaa atatctggaa gtaagctctg ttagcaactt
     1921 tggtgaattt caagcacgcc gagctaatat tcgctttaag gataaaacgg gaaaagttag
     1981 gcacttgcat actttaaatg gttccggagt tgccacccca cgtttgttga ttgctattct
     2041 ggaaacttat caacaagaag atgggacaat aaaactgcct tcagtgcttg atcctttttt
     2101 acgcttaact ttataataac ctcaggaaga ggagaataat gaataaaaac gaattgggct
     2161 tattggacat catcctggtg atagccaaac gcaaatatct ggtaataact atatgctgta
     2221 tagtggctgt ggcagctatt atatattcac ttgttgttcc tgaatattgg gaatcgaaag
     2281 caacgcttat gccaattgcc gaatcgggaa gtataggttc cttgggtgga agttttatgg
     2341 atattttagg gggaggactt ctacaaaccg acaaatatga tatggcagtg gatttcatct
     2401 acataatgca aagccgtaaa tttcgggaag aagtggtaag aaaatttaat ctgatccaat
     2461 attacaaaat aaaagaaccg gattcgctga aggcaatgga attggcagtg agaaaattaa
     2521 gtgaagagac aatggagatg ttttatgatc agaagaccta tctggtaaac attaaagcgg
     2581 aaaccaaaga tagagaactt tcccgtcaga tggtccaaca tcatttggat gccctgagca
     2641 aatacatttt aaatagcaaa atgagtaaag ggcgccagaa acgacaattc ttggaaaagc
     2701 aagtagagga gcataaacag ataatggatt ctctggcgat gcgaatgagg gatttccaaa
     2761 agaaaaaccg ttccatagat atcactcagc aaacgcaatc actgataaaa ctatatagtg
     2821 atgtagtggc tgaatatatg caagctgaac tggaatactc tcttgctctt acccaatatg
     2881 atgagaaatc acctatagta aagtctctaa cggagaagat gaaattattg aaaaagaaag
     2941 tgcagagttt ggagaaaaac aatagtgatc tggttcctga atatattgtg caaatagata
     3001 aaattccaga tatatcgatg caatatgctc aaatgatgtt gaatgtagaa atagagaagc
     3061 aaataattga atatctatat ccgcaatatg aacttgctaa gctggaagaa gtgaaagacc
     3121 ttcctacctt cgaaatatac gatgcacctc aaattgctgg tattaggtct aaacccaaaa
     3181 gagcaatcac tgtaattgtt agcactgtgg ctgcctttat tttttcctgt attttggcct
     3241 tgattttgga aagctttcag ggtgaaaata gagaaaaaat attggcaatt aaagcagctc
     3301 tctttagaaa aaggaaacaa tcctgaattc cgcaaccctg aatttatcgt ataataacat
     3361 cagattaaac tatctgatag cgggattatt attgatagcg gtagttctgg attactttcc
     3421 tgtggatatg ggctttaatg atatctattt tatcgcggtg gccagcctat gcttaattta
     3481 tgctataata gataaggata tcgcaataat caatgaagtt aatcttgttc cggtattgtt
     3541 tttattagca ttatcggctt attcactaat aagttataac tggtcgatag ataaaaggat
     3601 ttcgttagcc cattcaaaat taatcttaag aaaaaccgca ttatttctca taataagtgt
     3661 tttttgcaga gataaaagag tgaagcaata cgctcactgg ttctggtttt ttctaatgct
     3721 agcctatctt ggaattgctt tttgggaaat atctacctgg aaacacttac ctcaatcaat
     3781 ctattataac agggcgattt ttgttccaac gggacctttc tatggtccaa accaatttgg
     3841 agctgttttc tgtttgttat ttccgtttta tctttttatt cctgagctta caaaaaatag
     3901 aaggtactta agggtatttt cgtttatatg tgctttgttg gtcattgcca ttgttattgt
     3961 ggaaggagcc agaattgcca ttattgctac attggggatg ttgttatata cgctgttttt
     4021 tctttattcg aaaaaaatga gaagaacggg tcttttacta attggcctta ttattctggg
     4081 gctcattata tttgccaagc ctcttgtaaa ttttacaatt agtaaattga gcgctgaaat
     4141 tagcagtatt gggcaagaat caaaatcttg tgttatgggc agtataaaaa ttcgcactcg
     4201 cttaattcct gagaatctgg aaatattggc tcaaagtgga tttatgggtg tgggtgcagg
     4261 taatgtggaa cgctatatgc tttcgggaag gatctataga acaggtggaa tttccaatcc
     4321 acacaatttc tttctggaac tgatggcgaa ttatgggctt gtatttcttt taggctttct
     4381 ctatatttat ggaatctggc tctaccgtct ctggcgagct ataaaagcga atccggaaca
     4441 aaagaaatat tacgaaatgt ttttctttgt gcttttgatg tttatacctg ctgccagctt
     4501 acccagctcg ataatttggg aacatcatta ctggattcta tttgcgttta tcaatgaaat
     4561 atctcatcca gcaaactgga tgcaaaaggc aatatgagta aatttgaaaa acttgttgaa
     4621 attgtagcaa ctctcagaac tcctggaaca ggatgtccct gggatttaaa acaaacccgc
     4681 gaaactcttg ttcccaatat tattgaggaa ctatatgaag ttatcgaagc catagaaaat
     4741 aaggacgata actcgctgaa agaagaattg ggcgatcttc tattgcatat cgtttttcaa
     4801 gcacaaatat ctaaagaaca gggattatgg tctatagacg atgtgataga tgaaattatc
     4861 aataaactca ttcgtagaca tccacatgtt tttggagacc taaacataga agatgcagat
     4921 gcagtaaaag taaattggga acgccttaaa aagaaagaaa aaacaatgcg gaaaagtgtt
     4981 ttggatggta ttcctaaaaa aatgccggct cttattcaag cacaacgaac ccaagaaaaa
     5041 gctgccgctg taggttttga ctggcaagat attaagccca ttatggaaaa aatagatgaa
     5101 gagaaagagg aatttataga agcgttagcc agtaacgaag aggagaaaat acgcgaagaa
     5161 cttggagata tgatttttac tttggtaaac ttggcaagaa aattacatat cgatgccgaa
     5221 gcggctttaa aagaatgcac gcgtaaattt gttcgccgtt ttaatagtat tgaagaatac
     5281 taccgtaaag aaggaaagga tattaatgaa gccgatcttg aagagctcga ttctcactgg
     5341 caaagaatca agggacacta atctattcaa tcgccttagg tttattctaa tctttggcag
     5401 tttagccatc tttatctttt tcgggattta tatccaattt ttgataaaac aggcaaaaca
     5461 ggaacaagaa tatatacccc gcatctttgc tcaatatatt gcttacacag ataagtattt
     5521 aagagaagca gaactatatg ctcaattaat tacggaaata agctccaaat atttacaatt
     5581 tactacttcg agtgatttta aacaagacct gtgggattat atcagcacag agtttatgca
     5641 agataatcca gtcccagtta ttattaccga tcagaatttt cagccacaac tctggaaaaa
     5701 tgtaggagtg ccaactgata ctttgtttca tgaactctcg gcagcgaatc aacggaaatt
     5761 ggaaaatttg atgaaaacaa tgattcaaac ccccttaacg gataatggca aaatcagtgg
     5821 ctatgccttt tacagtaaac ctatctcttt tgcagaattt ataaaaaatg tggattgtgc
     5881 tattattgtt accgaccatt ataaacaacc tcttttctgg aggaatgtag gtattcctga
     5941 agctgctaat tactatgaaa tctctactga agaacaacaa aaattggctc accagattca
     6001 ggaaatggaa gaaatacctc tttccaatca ggcagacagt ttgggttata tttatttttc
     6061 gaatcctaaa tccctctcct atatacgcta tatagttatt ttggagcttg tttttgctct
     6121 aactgttgtt ttctttggag tatatggctt atttctactt agcagaacag aaaaggatac
     6181 gctttggata ggcttggcaa aagaaacagc tcatcaattc ggaactccaa taacctcatt
     6241 aatgggttgg ttggattatt taaaagaacc acctgtagat ggcagtgaaa gagatataaa
     6301 tcaaattgtg caatatatga ctgccgattt agaacatcta aaaactattg cttctcgctt
     6361 tgggaaagtt ggcagcgtta caaaactgga acgaaataat ttacatcaag ttattgctga
     6421 aacagtagag tattttcggg ctcgtatgcc tcacctggga aacaaaatag atatccatta
     6481 catcagcaag attgatgatg aaacagtttt gatggataaa gaactaatta aatggacatt
     6541 agaaaacttg attaagaact gtatggatgc aatgattact aaaggtggaa acataataat
     6601 tacagctacc aagaaagacc cctggatcta tattcatatt cgcgatgaag gtaaaggaat
     6661 tccacgcaga caatggaaac gaatttttga accaggggta acttccaaaa ccagaggttg
     6721 gggtttgggg ctcagcttag taaaacgcat tattgaagat tatcataacg gcagcataaa
     6781 agttctgcaa agcactattg gtgaaggcac cactttcgaa attaaacttc ctgttcaggg
     6841 aatcaataag gagaaataaa atgagctaca tttttacccc ttcacaaatg aaaacccttg
     6901 atgaaaatac gattaattgt tttggtttgc cagctagaat cctgatggaa aacgcaggta
     6961 aaggttgtgc agattatctg ctgcagaact gttcggaaca tttgaataat gtgattatta
     7021 ttttacatgg aacaggaaac aacagcggag atggctttgt tcttgcccgg tggctaacta
     7081 tctatggtag aagagtcctt ttatgtaaag ttcaggatgg cacgatgaca gaggaatgtc
     7141 gtctcaatta tgaactttgc caatcgctta acattccaat gattggatta gataatattt
     7201 ctgatactct ggagattctg gataacagct cttatattgg tatgattgtt gatgctgtgt
     7261 tcggaactgg ctataaaggt aaattaccta cttcgctaaa gaagatcttt cagagtgcaa
     7321 acaactcacc tgcatttaaa gtagcaatag atattccttc tggattaaat ggagctaccg
     7381 gagaaggaga ggatgctttt gaagcggact taactctttg tttacattcg cctaaaattg
     7441 gctctttgct acacacagga aaattgaaaa gcggagatct tgttacaatt ccgattggga
     7501 ttccggatag ttataatgat gcttttttct ctcccgctct tcatattgat gctgaaacat
     7561 ttcaagttcc caagcgttat ttgttggcta ataaatcaga ttatggtaga gtttttataa
     7621 taggcggttc tatgggactt ttaggttcgg tggcaatgtc tgccaaggct gctttaagag
     7681 cgggagctgg tttggtttat ttagtcagtc gcgaagaata tgcctatttc tataatacca
     7741 atccttccga agtattgttt gttggtattc cgtctaaacc tgaatctttg ctccctgatt
     7801 tgagtgaatt ttcttctctg ataaagaaag ctgatagcat cgttattgga ccaggtttag
     7861 gaacggatga atatgggtat attatgttgg agtttatatt aagaacttcc acagttccca
     7921 cggttgtgga tgctgatgct ttacgactaa ttgccaaaga gccatcttta caacagtata
     7981 ttaaaaaacc caatattctg cttactcctc attttggaga attttgtgac ctggctaaga
     8041 tagaaatgag tgctctctat aaagatacca tctctgagct cactaagtat gttaaaaata
     8101 cagaggcaaa agttctcttg aaaagtgata ctaccatctt ctacgatggg aaaagaatgt
     8161 tgattaatac cacaggtaat gatggacttg ctacaggtgg aagcggagat gttcttactg
     8221 gcataattgg ttccttttgc ggacaggata tggaattggg agaggcagct ataaatgctt
     8281 ccttttttat gggttttaca acggaatatc ttgctacaaa acgctatccc tgttcaattt
     8341 tgcccacaga tattatagac aatttgtttg taagacttgt tcaggaggga tcaaatgaat
     8401 aagcttgcgg ctttaaatac tccgattttc tgggcggaag gacatcctgg ttcgctaaac
     8461 agagaaacct ggaaaataga ggtctgcgga agttgtaata agccccaaat cttctcctgg
     8521 tctgatttaa tggaattacc ccaaagagat gttaagggcc gtttaacaag tgttaccaga
     8581 tggtctgtgg aaggtatttg gaaagggatt cctctttctg aaatattaaa aacagtgcaa
     8641 agtaacgatt cctgtaaata tcttcgcttt tggagttttg ggggaatata cgatacctct
     8701 atacctatcg atattgcttt taaagaaaaa tccctgcttg ctcatagttt cgagggggag
     8761 ttattaacag aagattacgg aggaccaatt agagctttca taccctatct ttggggctat
     8821 aaatctgcaa aaagcgtagt taaaatagag cttatggatt attatgtttc tggcttttgg
     8881 gaaaaccgcg gttatacaga tagcggagaa attgaagcag gaccttg
//
LOCUS       DAOSTH010000064         8856 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_7532_length_8856_cov_12.174526, whole genome shotgun sequence.
ACCESSION   DAOSTH010000064 DAOSTH010000000
VERSION     DAOSTH010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7532_length_8856_cov_12.174526"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /gene="rpsL"
                     /locus_tag="PLB65_06785"
     CDS             complement(<1..272)
                     /gene="rpsL"
                     /locus_tag="PLB65_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOQ78269.1"
                     /translation="MPTINQLVRKGRADVEKKRKNRALMECPQRRGVCTRVYTTTPKK
                     PNSALRKVARVRLVNGFEITAYIPGEGHNLQEHSIVLVRGGRVIDLP"
     gene            complement(311..4789)
                     /gene="rpoC"
                     /locus_tag="PLB65_06790"
     CDS             complement(311..4789)
                     /gene="rpoC"
                     /locus_tag="PLB65_06790"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HOQ78270.1"
                     /translation="MLKDKYEIKPNEYDAVRIKIASPDTIRHEWSHGEVTKPDTLNYR
                     TLKPEKDGLFCERIFGPERDYECACGKYKKKRFQGTVCDRCNVLVTTSRVRRTRMGHI
                     ELAVPIAHIWFVKSAPSKIGTLLDMTVKDLERILYYESFIVIDPGDSPYEKMELLEVD
                     EYYEIKDKLGDNFLALMGGEAIQELLKRINLKNEALDLRTRLKFEESALRKQKLINKL
                     KIVDAFIKSGNRPEDMILEVLPVLPPTLRPLVPLEGGRFATADFNDLYRRVITRNNRL
                     KQLLEIRAPEVILRNEKRMLQEAVDALIDNGRKARPVRGRGNRPLKSLTDQLKGKQGR
                     FRQNLLGKRVDYSGRSVITVGPELKLYQCGLPKEMAVELFKPYLIEKLQKMGEVDKVK
                     NAKKLIEKKQPEIWSILEEVIKDYPVMLNRAPTLHRLGIQAFMPVLTDNKAIQLHPMV
                     CVPFNADFDGDQMGVYIPLSLEAQIEARVLMLSTRNLLLPANGRLAMAANQDIVLGCY
                     YLTMEETPAPKDIEKLRHFYGPDEVIAAYEEEEQLSIAKGDSVFERNLDLHTWIRLKL
                     ESEYIITTVGRVIFNQIMPPEVGFQNYVFDKGKLNDLAMLCFDAVGQWRTAQILDKVK
                     ELGFSYATRAGVTFSFEDIIVPERKNDIVAQSEGEVKKINDLHQKGGITENERFGRVV
                     DQWKKTTVRVTDEMMEELSRTRNGLNSIYMMYKSGARGSKDQIKQLGGMRGLMDKPTK
                     IGATGGADIIETPIKSNFKEGLTVLEYFVSTHGARKGLADTALKTADAGYLTRRLVDV
                     AQNAIITIEDCDTTRGVNMTALKEGNEIVQTLAERIQGRTAVDDVVSPITGRVLVSAG
                     EEISNKMARTIQNHGIISVHVRSVLTCEAERGICAKCYGRNLATQKPAKLGDPVGIIA
                     AQSIGEPGTQLTLRTFHIGGAASTATELAEVVASHDGIVKFDRMNTVTNPENQLISVS
                     HLGRILIVDEQDDSKVLEEFKVEYAATVHVQDGQKIAKDTKLLSWDQFNNPLISTAKG
                     VLHYENFIKDITYKEEYNDITLARELTIIESKDRRKQPQFRIVGEDGTVRLIPLPTGL
                     IVRVEDGSFVHTGDILGQTSRMTIKQRDITGGLPRVQELFEARVPKDKAKISDIDGIV
                     TIGGLKKTGRDIFVTPPNGLFAPFDGKVSIYSDEDKNQYIAVLSDKAVLSENDGKVTI
                     LEENRKKIIQVAKRGQKPVQYSVPKGMQILVKEGESVKTGTPLCGKLYAVPPEQESIV
                     STGDSVSIHQPLAGRKYIIPSGKRIIVHQGDYVESGDALSDGPLDPHDMLVKGVIEAQ
                     ILILNEIQEIYRKQGVKIDDKHVSVIIRQMFKKVRITDSGSTSFLQGDIVDKILVEKE
                     NREAISFGKTPAQFEQLLLGITKTSLLTDSWLSAASFQETTKVLTKAAIEGRVDHLEG
                     LKESIIIGHRIPVGTGAKLYNNMIKQAVNEGKTVAQIIDEYAHHAEEEESEDYLNF"
     gene            complement(4804..8583)
                     /gene="rpoB"
                     /locus_tag="PLB65_06795"
     CDS             complement(4804..8583)
                     /gene="rpoB"
                     /locus_tag="PLB65_06795"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HOQ78271.1"
                     /translation="MRKVKSYSRISGRFAELGIPEVEIPNLLAMQVDSFNEFLQKDIH
                     PQRREKKGLQAVFENAFPLEDAKGNFLLEFVEYSVLQEKYTIEECRDRNLTYQAPLKA
                     KMRLSVFENNEGVREHKDTLEQEVFLGEIPLITEQGTFVINGAERVIISQLQRSPGVF
                     FSEEKHPSGKTLYSAKVIPYSGSWLDFDIDIHDVMYVHIDKRRKLPVTTLFRAIGIST
                     NEDLRKTFYKTEQLNLKSAKGRHLYADIYETDNPEPIALANEQITDSLIKFLTERKIT
                     KVEVIDYNYEIARKVLENTLAKDPTTNQEEALKKIYALIRPGEDAPIEVAKQLVDRMF
                     FNEKRYNLGEVGRYKMNTRLGINVDPKVMTLTVEDFVYIFKTLINIYHDEDEVDDIDH
                     LSNRRVRTVGELLQEQYTSGMAMISRIIQERMAISNIDEVTVHDLVNSNALISVVQSF
                     FLTGQLSQFMEQTNPLAALRHKRALSALGPGGLTRERAGFEVRDVHSSHYGRVCPIET
                     PEGPNIGLIVSPALYSRINYFGFIETPYRKVVDGCVTDEFEYLDAAQEEKYIIAQSDI
                     HLDDNRRITDDVIFARDRGDFVQVNPKNVQYMDIAPQQMVSVSAALIPFLEHDDANRA
                     LMGSNMQRQAVPLINPQAPLVGTGMEKIVSMDTSAIAVAPYDGIVTQVTSAFIDLKPI
                     SEKDEAFYLSIGNRIYLKKFVRTNQDTCINQRPIVRIGDNVKKGQPISDGACVEDNRL
                     ALGTNMMVAFMPWYGYNYEDAIILSEKVARDDSLTSIYIEELEVLVRNVKNGREELAY
                     DIPNVPAQALKNLDKTGIIRVGSVIRAGDVIVGKVTPKSVEIDPSPEENLMRALFGDR
                     AGDFTNSSLKAKPGMEGVVIDVKVFSRLEEGSDLEEENEDRIEKLKEELALRRRKIEE
                     FKEEKLSSVLVGQIAKNIWDEKTNAPFIAPGKKITKEDIKRINFKKLDLDVELVEDSE
                     VNNYIYYQIVLKIKQSLEQSDNIYKKSRERIKHGDELQYGVRKMVKVYVAKKRKIEVG
                     DKMAGRHGNKGVISIVAPVEDMPFMEDGTPVDIVLNPLGVPSRMNIGQIMETHLGWAA
                     KTLGFEVETPIFDGASNEDIRSELRKAGLPEDGKTVLYDGKSGEPFKERVTVGVIYMM
                     KLNHLVADKMHARSTGPYSLITQQPLGGKAQHGGQRLGEMEVWALEAYGASHVLEEML
                     TIKSDDVDGRNNAFKAITKGENPPPPGVPESFNVLISELKSLGFDIEFIKEEERR"
BASE COUNT         2471 a         1928 c         1507 g         2950 t
ORIGIN      
        1 ggaaggtcta ttacacgacc tccgcgcact aaaacaatag agtgctcttg taggttgtga
       61 ccttctccag gaatatatgc tgtaatttca aatccgttta caagacggac acgggcaact
      121 tttctaagag ccgaattagg ttttttgggc gtagttgtat atactcttgt gcatactccg
      181 cgcctttgcg gacattccat aagcgccctg ttctttcttt tcttttcgac atcggctcta
      241 cctttacgaa ccaattgatt aatggttggc actgattttc tccttttatc ttattgagtt
      301 atagtgtatt ttagaaatta aggtaatctt cactttcttc ctcttccgca tgatgagcat
      361 attcatcgat tatttgggca acggttttac cttcattcac tgcttgttta atcatattat
      421 tatagagttt tgcccctgtt cctacaggaa tacggtgacc aataatgatg ctttctttca
      481 gaccttccag atgatcaact cttccttcaa tagcagcttt tgtgagtact tttgtggtct
      541 cctggaacga agcggctgaa agccagctat cggttaacaa agaagtttta gttattccca
      601 gaagcaattg ttcaaattga gcaggtgttt taccgaaaga tattgcttcc ctattttctt
      661 tctccaccag aatcttatct acaatatcac cttgtagaaa actggtgcta ccactatcgg
      721 taattctaac ctttttgaac atctgacgaa tgattacgct aacatgttta tcgtcaattt
      781 tcacgccctg tttacggtat atttcttgaa tttcattcag aataagaatt tgagcttcga
      841 taacaccttt cactaacata tcgtgaggat ctaatggacc atctgataaa gcgtctccac
      901 tttccacata gtcaccctgg tgaacgataa ttcgttttcc agaaggtatg atatactttc
      961 taccagcaag cggctggtga atagaaacgc tatcaccagt tgaaacgata ctttcctgtt
     1021 caggtggaac tgcgtatagt ttaccgcaaa gaggagttcc cgttttaacg gattcacctt
     1081 ccttaactaa aatttgcatc cctttgggta cagaatattg aaccggtttc tgaccgcgtt
     1141 tagctacctg aattatcttc ttgcgatttt cttctaagat agttacttta ccatcgtttt
     1201 cactcaatac tgctttatca gaaagcactg caatatactg atttttatct tcatcggaat
     1261 aaatagatac ttttccatca aatggagcaa aaagtccatt aggaggagta acgaaaatat
     1321 ctcttccggt ttttttcaag ccaccaattg ttacaattcc atcaatatcg gagatcttgg
     1381 ctttatcttt gggaaccctt gcttcaaaaa gttcctgcac tctgggtaac ccacctgtta
     1441 tatcacgttg ttttatagtc attctggagg tctggcctaa aatatcgccg gtatgcacaa
     1501 agcttccatc ttccacgcga acaatcaatc ctgttggtaa aggaattaac cgtacagttc
     1561 catcttcgcc aacaattctg aactgcggtt gttttctacg gtctttggat tcaataatag
     1621 taagctctcg agcaagagtt atatcgttat attcttcttt ataagttata tccttaataa
     1681 agttttcata gtgcaacaca ccctttgctg tagaaataag gggattattg aactggtccc
     1741 aagaaaggag cttcgtatct ttggcaattt tttgaccatc ctgaacatga acagttgccg
     1801 catattctac tttaaattct tctaacactt tactatcgtc ctgttcgtcc acaatcaaaa
     1861 ttcgacctaa atggctaacg gagataagct gattttcggg attggtaact gtattcatcc
     1921 tatcgaattt tacaatacca tcatggctgg caacaacttc tgccaattct gtagctgtag
     1981 aagcagctcc tccaatgtgg aaggttctaa gtgttaattg tgttccaggt tcaccaatac
     2041 tttgagcggc aataatacct actggatcac ctaattttgc aggtttctga gtggcaagat
     2101 tgcgtccgta acattttgca catattcctc tttcagcttc acaagtaaga acactgcgaa
     2161 catgtacaga aattatacca tggttttgga tagttctggc catcttgttg cttatctctt
     2221 cacctgcaga aactaaaact cttcctgtga tgggacttac aacatcatcc acagcagttc
     2281 ttccctgaat tctttcggcc agggtttgta caatttcgtt gccttctttg agggctgtca
     2341 tattcacccc tctggtagta tcacaatctt caattgtgat aatagcattt tgggcaacat
     2401 ctactaacct acgggttaaa tatcctgcgt cagctgtttt taacgctgtg tctgccaatc
     2461 ccttacgcgc tccgtgagtg gatacgaaat attcaaggac agtaagccct tctttaaagt
     2521 tactcttgat cggagtttca ataatatcgg ctccacccgt ggcacctatt tttgtaggtt
     2581 tatccatcaa accacgcatt ccacccaatt gtttaatctg gtctttactg ccgcgcgctc
     2641 cagatttata catcatataa atagagttca agccatttct tgttctggag agttcttcca
     2701 tcatttcatc tgtaacccgc actgtagttt ttttccattg gtcaacaact cgtccaaaac
     2761 gttcgttttc ggtaattcca cctttttgat gaaggtcgtt tatttttttt acttcccctt
     2821 cagattgagc aactatatca tttttccgtt caggaacaat tatatcttca aaactgaatg
     2881 ttactcctgc tcttgtagca tagctaaaac caagttcttt aactttgtcc aaaatttgtg
     2941 cagttctcca ttgtccaaca gcatcaaaac ataacatagc cagatcgttc agttttcctt
     3001 tgtcgaaaac atagttctga aaacctactt ctgggggcat aatctgattg aaaataaccc
     3061 tgcctacggt tgtgataata tattcggatt ccagttttaa gcgaatccaa gtatgtaggt
     3121 ctaaattccg ttcaaagaca gaatcacctt tagctatgct taattgttct tcttcttcgt
     3181 aagccgcaat aacttcatca ggaccataaa aatgacgcaa tttttcaata tctttgggag
     3241 caggagtttc ttccattgtt agataatagc atcccaaaac gatatcttgg tttgcagcca
     3301 tagctaaacg tccgtttgca ggtaataaca gattgcgagt agagagcatc aatactctgg
     3361 cttcaatttg agcttccaga gaaagaggga tataaacccc catttggtca ccatcaaaat
     3421 cagcattaaa aggaacacaa accattggat gtaattggat agctttgtta tctgttaata
     3481 cgggcataaa tgcttgaata cctaaacggt gaagagttgg agcacggttt agcataacag
     3541 gataatcttt tattacttct tccagaatag accatatttc tggttgtttt ttctctatta
     3601 gttttttggc attttttact ttatctactt cccccatttt ttgtagtttt tcaataagat
     3661 agggcttaaa gagctcaact gccatttctt tgggaagccc acattgataa agttttagtt
     3721 cgggaccaac cgtaataacg ctacggccag aataatctac tcgcttaccc aaaagatttt
     3781 gacgaaaacg cccttgttta cccttaagct gatctgttaa tgattttaag ggacgatttc
     3841 ctctgccccg tactggacgt gcttttctac cattatcaat taaagcatca actgcttctt
     3901 gtaacattcg tttttcattg cgtaaaataa cttctggagc acggatttct aaaagctgtt
     3961 tcaaacggtt atttctggta ataactcttc tatataagtc attaaaatca gcagttgcaa
     4021 agcggccacc ctccaaagga actaatggtc ttaaagttgg cggaagcaca ggtaaaactt
     4081 ccaaaatcat atcttcggga cgattaccac ttttgataaa ggcatctact attttaagct
     4141 tattaatcag cttttgttta cgcaaggctg attcttcaaa tttaagacga gtcctaaggt
     4201 caagcgcttc attttttaag ttaatccgct tgagcaattc ttggattgct tctccaccca
     4261 taagggccag gaagttatct cccaatttgt ctttaatctc gtaatattca tcaacttcca
     4321 gcagttccat tttttcataa ggactatcgc ccggatcaat aacaatgaag gactcgtaat
     4381 aaagaatcct ttcgaggtct ttaactgtca tatcaagtaa agttccaatt ttgctaggag
     4441 cacttttcac aaaccagata tgagcaatag gcactgctaa ttcaatatgt cccatacgag
     4501 ttcttcgtac gcgagaagtt gttactaaaa cattacaacg atcacaaaca gtgccctgaa
     4561 agcgtttctt tttatatttt ccgcaagcgc attcataatc ccgttccggt ccaaatattc
     4621 tctcacaaaa caaaccatct ttttctggtt taagagttcg ataatttaga gtatcgggtt
     4681 ttgttacttc tccgtgggac cattcatgtc taatagtatc aggagaagca atctttatgc
     4741 gaactgcatc atattcgttt ggcttaattt catatttatc ttttaacatc ctatttccct
     4801 ccgttacctt ctctcttctt ctttgataaa ttcaatatca aagcccagtg acttcagttc
     4861 actgatgaga acattaaagg attcaggcac tccgggagga ggtggatttt cgcctttagt
     4921 aatagctttg aatgcattgt tacgaccatc cacatcatca cttttgattg ttagcatttc
     4981 ttccagaaca tgagaagctc cataggcttc caaagcccaa acttccattt ctcctaatcg
     5041 ctgaccacca tgctgagctt ttccacctaa gggttgttga gttataagtg aataaggtcc
     5101 cgtggaacgt gcatgcattt tatcggctac taggtgattc agtttcatca tatagataac
     5161 tcccaccgtt accctttctt taaaaggctc accagattta ccatcataaa gaactgtctt
     5221 gccatcttct ggtagacctg cttttcgcaa ttcggaacga atatcttcat tggatgcgcc
     5281 atcaaaaata ggggtttcaa cttcgaagcc aagggtcttt gctgcccatc ctaaatgagt
     5341 ttccataatc tgaccgatat tcatacgaga tggaacacct aaaggattta gtactatatc
     5401 tacaggcgtg ccgtcttcca taaaaggcat atcttcgaca ggggcaacaa tagatattac
     5461 acctttatta ccatggcgtc cagccatctt atctcctact tcaattttgc gttttttggc
     5521 tacatatacc ttaaccattt tacggacgcc atattgaagt tcatcaccat gtttgattcg
     5581 ttcccgtgat tttttataaa tgttgtcgct ttgttcgagt gattgtttga ttttcaggac
     5641 tatctggtaa tagatataat tattcacttc actatcttca actaattcaa catccaaatc
     5701 gagttttttg aagttaatgc gtttgatatc ttctttagta attttcttac caggagcaat
     5761 aaagggtgca ttggttttct catcccagat atttttagca atctgtccca caagcaccga
     5821 tgaaagtttt tcttccttaa attcctcaat tttcctacgc cgcaaagcta attcttcttt
     5881 cagcttttca attctatctt cattctcttc ttccaaatcg ctgccttctt ccaaacggga
     5941 aaacacctta acatcgataa ccactccttc cattcccggt ttggctttta aagagctatt
     6001 ggtaaaatca cctgctcggt ctccaaacaa agcacgcatc aaattttctt ccggagaagg
     6061 atcaatttct acacttttag gagtaacctt acctacaatt acatctccag ccctaatcac
     6121 actacctact ctgataatgc cagttttatc aagatttttc aatgcctgag caggaacatt
     6181 aggaatatcg taggccaatt cctcacgccc gtttttcaca ttccttacca agacttccaa
     6241 ttcttcaata taaatggaag tgagagaatc atctcttgct actttttcac ttaaaataat
     6301 agcatcttca taattataac cataccaagg cataaaggca accatcatat tggtccctaa
     6361 agccaaacgg ttatcttcta cacaagctcc atcagatata ggctgcccct ttttaacatt
     6421 atcacctatg cgaactatag gacgctggtt gatacaagta tcttgattag tacggacaaa
     6481 ctttttcaga taaatccggt ttccgatgga taaatagaac gcttcatctt tctcactaat
     6541 tggttttaga tcaataaaag cagaagttac ttgagtaaca attccatcat agggagctac
     6601 agcaattgct gaggtatcca tagagacgat tttttccata ccagtcccaa caagaggtgc
     6661 ttgaggattt atcagaggaa ctgcctgtcg ttgcatattt gaacccatca atgcacggtt
     6721 tgcatcatca tgctctaaaa atggaatcaa agctgccgat actgaaacca tctgttgagg
     6781 tgctatgtcc atatactgaa catttttagg gttaacttga acaaaatcac ctcgatctct
     6841 ggcaaaaata acatcatcgg taattctacg gttatcatcc aaatgaatat cgctttgggc
     6901 tataatatat ttctcttctt gggcagcatc aagatattca aattcatcag ttacacatcc
     6961 atccacaacc ttccggtaag gagtttctat gaagccaaaa tagtttatcc tggaatataa
     7021 agcaggagaa actatcaatc caatatttgg tccttccggt gtttcaattg gacacacacg
     7081 tccatagtga gatgaatgaa catctcgaac ttcaaaacca gctcgctcac gagttaaacc
     7141 tccaggacct agagcagaaa gagcacgctt atgtcttaaa gctgcaagtg gattagtctg
     7201 ctccataaat tgagataatt gacctgttaa aaagaatgat tgaactactg aaataagagc
     7261 gttactattt acaagatcgt gcacagtaac ttcatcaata ttggaaatgg ccatccgttc
     7321 ttgaattatt ctggatatca tagccattcc tgaagtatat tgttcctgca gcaattcacc
     7381 aacagttcgt actcttctat ttgataaatg atcgatatca tccacttcat cttcatcgtg
     7441 atagatgttt attaaagttt taaagatata tacaaaatcc tcaaccgtaa gagtcatcac
     7501 tttgggatct acatttatac ctaagcgggt attcattttg taacgaccga cctctcccaa
     7561 gttgtaacgc ttttcattga aaaacattct atctacaagc tgtttagcaa cttctatagg
     7621 cgcatcttca ccagggcgta ttaaggcata gatttttttc agagcttctt cttggttggt
     7681 ggttgggtct tttgccagag tattttccaa aacctttctg gcaatttcat aattataatc
     7741 aatcacttcc accttagtga tttttctttc agttaaaaat tttatcaagg aatcagtgat
     7801 ctgctcgttt gctaaagcaa taggttcggg attatcggtt tcatatatat ctgcatagag
     7861 atggcgacct ttagcacttt tcaaattcag ctgctcggtt ttgtaaaagg tctttcgcaa
     7921 atcctcatta gtggatatcc cgatagcacg aaaaagtgta gttactggaa gcttacgacg
     7981 tttatcgata tgcacataca tcacatcatg aatatctata tcaaaatcca gccacgaacc
     8041 gctgtaggga atcacttttg cagaataaag tgtttttcca ctgggatgtt tctcttcaga
     8101 aaagaacact ccgggcgaac gttgtaattg tgaaataatg acacgttctg ctccattgat
     8161 aacaaaagtt ccctgttccg taatcagagg gatttctcct aagaaaactt cttgttctaa
     8221 agtgtcttta tgctcccgaa caccttcatt attttcaaac acacttagac gcattttggc
     8281 tttaagaggc gcctgataag tgaggtttcg gtcccgacat tcctcaatgg tatacttttc
     8341 ctgcaaaacg ctatattcaa caaattctaa cagaaaatta cctttggcat cttccaaggg
     8401 aaatgcattt tcgaatacgg cttgcaggcc ttttttctct ctgcgttgcg ggtgaatatc
     8461 cttttgcaga aattcgttga aggaatccac ttgcatagcc agaagattgg gtatttctac
     8521 ctccggaatc ccgagttcag caaatcttcc ggatattcgt gaataacttt taacctttct
     8581 caaaagctct ctccttttag gtgtttattt cctaacattt tgggctcttt ttgttaatct
     8641 caattttgct cacgcttgtt gttgcgccat tgatgtatgg aaaattggtt tgctggctta
     8701 tagttaaaaa aaccatcatt tatggataat tggattggga aattaggcca aaaaaagtcc
     8761 cgcaggactt tttttggacc tttatcctga ttatggttcg attatttgac ttcgacagtt
     8821 gcaccggctt cttcgagttt tttcctaatt gattct
//
LOCUS       DAOSTH010000065         8748 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_7647_length_8748_cov_11.862188, whole genome shotgun sequence.
ACCESSION   DAOSTH010000065 DAOSTH010000000
VERSION     DAOSTH010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8748
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7647_length_8748_cov_11.862188"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..619
                     /locus_tag="PLB65_06800"
     CDS             116..619
                     /locus_tag="PLB65_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78272.1"
                     /translation="MKSVKLAIFFIVGIAFFTSNIYGQISQPEDQNNTYPINTVNSFL
                     EGAAAGKADAKGNIVYGIGSIFCGPLGIVGAVISDPQPEPTKVNHLLQSKGSDYVLAY
                     TTAYTTESRRLNLVYSAVGWGINTFIASVYYFHIVHDIDSDFDDWFKNQRKIFISVPL
                     KNPAHPY"
     gene            1158..2279
                     /gene="mnmA"
                     /locus_tag="PLB65_06805"
     CDS             1158..2279
                     /gene="mnmA"
                     /locus_tag="PLB65_06805"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOQ78273.1"
                     /translation="MQNKICVAVGISGGIDSAMTAYLLKESGYRIIGITMQTWDKNIA
                     MEKVLKSGCFGPAQEESIRASAILCQKMGIEHYVIPLQEEFQQKVINYFCQTYLQGET
                     PNPCLVCNAYIKFALLPQKAKLMGLEFDYFATGHYAKVDYNKELQRYQIKRARDKNKD
                     QSYFLAFLSQEQLAGTLFPLGDYTKPEIKKMAAEIGFGELVTRQESQDFLQSSDLEVL
                     FKAEEYQQGEIVDINGKIMGTHKGIINYTIGQRRNLGVSGLPEPYYVLKIDAINNRIV
                     LGTQQYLYKNRCKATGMNWVSISAPEKQFNAKAKIRQQHTPADCIVTPIDDRNIEVVF
                     AKPQLSITPGQGLVLYDEDLLLGGGFIALSNNDDAEHNA"
     assembly_gap    2588..2687
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3049..4782)
                     /locus_tag="PLB65_06810"
     CDS             complement(3049..4782)
                     /locus_tag="PLB65_06810"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HOQ78274.1"
                     /translation="MASKSSVEYYSNIKEMSPIRAIAETLMNNHLVRKVDIREAYELA
                     IKQPGVSVTDLPMYPEFVKLHNLPKDAKIINDCHGNIIGRTAKARRFYNRVNPSLKNK
                     LESDFREAVWQMQHYPLIKAEAILGMDKDLMLKATFITTESDAANLYFWLLNFAPYES
                     LAQEYEASPKLPIQDIILVAFNEWTCNDSYYNNVGAPQLALVDEKHNVMVNLGMRYFG
                     ERKKGTLTLAWTSGIRIGMAACHGGIKEIDFGTCENPEYHKLGKRSIAFYGLSGTGKS
                     SHTNNADNAGTLPKGFSKVVLHDDAFQIDLENKQCRAWEPTLFDKTDSRPVDHPDWKY
                     ALALMNHTVLETNGKLIPYGQDIRNSNGRALLDRALLGNYVNRCGFPKALVWLMKDSI
                     LPPVLKLENKYLAIAMGAALMTQRNRAENVTEEELNKLVFEPFANPFRVYELYRDVEA
                     FLKVADSGADFYCFNSRGYWKESDDILEPIPLNTSLTLQTAILMDQLKWEHWNILPGA
                     MIPTRESIEAIIPGYYDKYNSTMRGNYDKYIALIKDRFQQRKDFLQSSDLTEKPDIQT
                     KLIQSLQVNAD"
     gene            complement(4844..6022)
                     /locus_tag="PLB65_06815"
     CDS             complement(4844..6022)
                     /locus_tag="PLB65_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HOQ78275.1"
                     /translation="MSLLDKCYKFTDARKVMAMGFYPYFREITSEQDTEVICNGKKML
                     MMGSNSYLGLTNHPLVKEAAIKAIQKYGSGCAGSRFLNGTLDIHLQLENELAKLVGKE
                     AAICYPTGYQANVGCISTMVGKNDFVITDKYDHASIIDGALLAEGKMLRYKHNDMASL
                     EHLLKDIGSKNSLIVVDGIFSMEGDIANLPAICDLAEKYNSEVMVDEAHSLGVLGNLG
                     AGTTAHFGLTDKTAFIMGTFSKSLASVGGFIAADEALIHYLKHKSRALIFSASLPPAS
                     TASVLAALKIMEEEPERIAKLWENTNYMLSEFKAMGYNTGDSCTPVVPLHVGNMMTAF
                     NMWRRLGEEGVFINPVVPPAVPPSSCLIRTSFMATHTQDQLDMALDKFRMIGKELNII
                     "
     gene            complement(6034..7065)
                     /locus_tag="PLB65_06820"
     CDS             complement(6034..7065)
                     /locus_tag="PLB65_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOQ78276.1"
                     /translation="MLFAIFILAVVFYHSIIYYLVYALIFSYILDPFVNWLEYHHFPR
                     WLAVITIYLIIAGIIAWFTSRLVPALVQQGNNLFNILASGEQLNGQYIITLPFISRIY
                     EFLVELDTKIPGLGIAPKMIEILDQTNVFLAKIPKLLIDNYKRIFSTISIIATVPLMS
                     FFILKDKYKLRKGILSLFNNRYFELCIIMFRKIDSTIGNFFRAILFEIIAVGILASTA
                     LTIVGVPNSVLIGATAGVANIIPYFGPFLGGALAVATVLINGSSILYAIYVIIAMYIV
                     QLIDNNIVYPVVVGGTIKMHPLLVLLTLIAGGWYGGILWMLISVPLVYITYNLIKVLY
                     VNLKDFKII"
     gene            complement(7095..7577)
                     /locus_tag="PLB65_06825"
     CDS             complement(7095..7577)
                     /locus_tag="PLB65_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOQ78277.1"
                     /translation="MLKDVFAWLKTIMKKPDKYADLKLFPVFNECSSFELYLLSNFLH
                     KRTYQAGEILYDKGYPLEVIYFVLSGEIEVTGPTHPQGCYVIGKNQVLGLIDMFSEET
                     RSSSAKAITDVTTYVISRFDLLDLAKQNPHLGVKLLYAFCQTLSNYILTISAIKAKIE
                     "
     gene            complement(7581..8231)
                     /locus_tag="PLB65_06830"
     CDS             complement(7581..8231)
                     /locus_tag="PLB65_06830"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HOQ78278.1"
                     /translation="MRFEDQRLKLVNELKLEGITSPAVLDAFAKVPRENFVLPEYREY
                     SYQNRPLPIKEGQTISQPLMIAIMLQLLDLKSSDTVLEIGTGSGYQSALLAEIVKEVC
                     TVERLESLSLNAQKVLRNAGYNNIYFRIGDGHSGWQKAYPPHKEFDKIIVSAAAQDIP
                     NRLLEQLVIGGKLAIPIGRAGFQILHIVNKTEDGYTITQAGGCSFVPLVCDNNNLG"
     gene            complement(8234..>8748)
                     /locus_tag="PLB65_06835"
     CDS             complement(8234..>8748)
                     /locus_tag="PLB65_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOQ78279.1"
                     /translation="IISGINAGQNMGEDVLYSGTVGAAMEAALLGNRAIAISITSYKN
                     QRFDVAAHWMRKLLDLGIDKQAEEYGVLNINVPNLPIEEIKGIRLTRTGHRKYYNFVK
                     VIKENDDGFVYEVGGDIPVWDNQTGSDAEAVNNGYISLTPLGFELTKSDTYPRILNWL
                     EENKLLQLES"
BASE COUNT         2828 a         1605 c         1519 g         2696 t
ORIGIN      
        1 aaggctcctg taaaaaaata tgggaatgcc aactgatttt ttccttgaca gaaattgtgt
       61 ttatggaata ggataaaaca agagtattat aacaaaacca agaggagttg gacttatgaa
      121 atcggtgaaa ctggctatct tctttatagt tggcattgca ttttttacga gtaatattta
      181 tggacagatt agtcagccag aagaccaaaa caatacttat ccaataaata cagtgaactc
      241 ttttctggaa ggagcagcag caggtaaagc agatgcgaaa ggaaatatag tttacggaat
      301 tggaagtatt ttctgtggac ctcttggtat agtaggagct gttatatctg atcctcaacc
      361 agaaccaacg aaagttaatc atttacttca gtccaaaggc agtgattatg ttctagcata
      421 tactactgca tacactacag aatctcgtag gcttaattta gtatattctg cagtaggttg
      481 gggaataaat actttcattg ctagtgttta ttatttccat atagttcatg acattgattc
      541 agattttgat gattggttta agaatcagag aaagattttt atttccgtgc cacttaagaa
      601 tcctgctcat ccttattgat ttaaacttca ataatacaat tttaaatgct gagaatatga
      661 tgaagagccc tgaatcaaat ctaaaattct cttcttcatt cacaagttac tcaatcgtaa
      721 tcagttatta tcagcaatcc acttcgccaa cctacgggca aagatgtctg atgtacccaa
      781 gttgttctca ttatacagcg taaagtagca aaaaagacat agagctttta aaggaatttt
      841 aaaaagtgac agataggatt atgcggtgca gattagatct tcatttttac ccttctaaat
      901 atatagatat gaggaaatac tatatagatt taccagaaga gggcattggg gataaggata
      961 aatgaaatat tggttgacaa taaccggaat attgttatta tcttatctat cagcataaat
     1021 aaattttgag gcacgaagta ataatattag gcaaaagaac ataattgata agcagttgga
     1081 tgttatcttt gacgaattaa taaaagtgta aagcagatag aactgtatga aaataaactc
     1141 tgaggttagt gaagaatatg caaaataaaa tctgtgtagc agtcggaatt agtggaggga
     1201 ttgattcagc tatgactgcc tatttattaa aagaaagcgg ttatagaata attggtatca
     1261 cgatgcagac ctgggataaa aacatagcaa tggagaaagt gttaaaaagt ggttgttttg
     1321 gtcccgctca agaagaaagt attcgtgcct ctgctatttt atgccaaaaa atggggatag
     1381 agcattatgt tattcctttg caggaagaat ttcaacagaa agtgattaac tatttttgcc
     1441 agacctattt acagggagaa acacctaatc cgtgtttggt ttgtaatgct tatatcaaat
     1501 ttgccttatt accccaaaaa gctaagttaa tggggttgga atttgactat tttgccacag
     1561 gccactatgc taaagtagat tataataaag aattgcaacg ctaccaaata aaaagagcaa
     1621 gagataaaaa taaagatcag tcctattttc tggctttttt gtcacaggag caattggcag
     1681 gaactctttt tccactagga gattacacaa agccggaaat aaaaaaaatg gctgctgaaa
     1741 taggttttgg cgaactcgta actcgtcagg aaagtcagga ttttttacag agttcagatt
     1801 tagaagtgct ctttaaagcg gaagaatatc agcagggaga aattgtagat ataaatggga
     1861 aaattatggg aacgcacaaa ggcataatca attacactat cggccagaga agaaatttgg
     1921 gcgtaagcgg tttgcctgaa ccctattatg ttctgaaaat tgatgccata aataatagaa
     1981 ttgttttggg aactcagcaa tatctctaca aaaatcggtg taaagccaca ggtatgaatt
     2041 gggtttccat ttctgcaccg gaaaaacaat ttaacgccaa agccaaaata agacagcaac
     2101 atactcccgc cgattgtatt gtaacaccga tagatgatag aaatatagaa gtggtttttg
     2161 ccaaaccgca actttccatt actccaggac aaggcttggt tttatatgat gaggatttac
     2221 ttttgggggg tggatttatt gcactttcca ataatgatga tgcagaacat aatgcttaga
     2281 gggttttagt agtttagaag gttgagattt tttaatagct tgatagtttg tttattgtgg
     2341 ttggctagga cttcaacctt ccgttatttt tacgccgggt taaaaacccg tcgctaaaat
     2401 ctgtcgtccc gatgggacta aattatttgt tttatatttc gccgggttaa aacccgtcgt
     2461 taaaatctgt cgtcccgatg ggactaaatt atttgtttta tatttcaacg ggttaaaacc
     2521 cgtcgttagt atgtatcgtt cctaacggaa ctttaaaaat cctctaaatc ctataatcct
     2581 aaaatccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaat ccctgtttct
     2701 aaaatcaaat ctgcgtcatc tgcgtaatct gcgtgaacta gtcctaaaat ccttgtttct
     2761 aaagctgtaa acctataatc cttgcttgat tacttatggt agagatgcgg tggtgtttct
     2821 cgggaaaata cctctgatca gcaccatagc tccatgatag ctaattaagg aaggataaaa
     2881 aagatagcgg aaaattaatc ccgctatcta tgaaacattt tttagggcaa gttactcttt
     2941 actgcatagt tactcgggct gcaaaagaat gacatccttg gcatttggcc aatcagaatt
     3001 tgggcaaccc ttttttatgc agggaataag tatgccagcc aaaaagttct aatcagcgtt
     3061 tacttgcaag gactggatca attttgtctg gatatccggt ttttcggtta aatcgctact
     3121 ttgcaaaaag tcctttcttt gttggaatct atctttaatc aaagcaatgt acttatcata
     3181 gttaccgcgc atagtggaat tatatttatc ataataaccg ggtataatgg cttcgatgga
     3241 ctcccgagta ggaatcatcg ctcccggaag tatattccaa tgttcccatt ttaattgatc
     3301 catcaaaatg gcagtttgaa gagtgagaga agtatttaga ggaatgggtt caaggatatc
     3361 atcagattct ttccaatagc cacgggaatt gaagcagtag aaatctgctc cgctatcagc
     3421 tactttcaaa aatgcttcca catcccgata gagttcataa accctaaaag gattggcaaa
     3481 aggttcgaaa acgagtttat tcaattcttc ttcggtaaca ttttcagcac ggttgcgctg
     3541 agtcattaat gctgctccca tagcgattgc caaatattta ttctccaatt ttaaaactgg
     3601 aggtaaaata gaatctttca tcagccaaac caaggccttg gggaaaccgc aacggtttac
     3661 ataatttccc aaaagagcac ggtctaataa cgctctacca ttggaattac gaatatcctg
     3721 tccgtagggt ataagtttgc catttgtctc taacacggta tgattcatta aagctaaggc
     3781 atatttccaa tccggatgat ccactggccg gctatctgtc ttatcaaata aagttggttc
     3841 ccatgcacgg cactgcttat tttcgagatc aatctggaaa gcatcatcat gcaaaacaac
     3901 cttggaaaag cccttaggta aagttccagc attatcggca ttatttgtat ggctggattt
     3961 tcctgttcca gaaagtccat aaaaagctat agaacgctta cccaatttat gatattcagg
     4021 attttcacaa gtaccaaaat caatttcttt aataccacca tggcaggcag ccatcccaat
     4081 tcggatacca gaagtccagg caagagttaa agttcctttt ttgcgttccc cgaaataacg
     4141 catcccaagg ttgaccatta cattgtgttt ttcatctact aaagccagct gcggtgcgcc
     4201 aacattgtta taataagaat cgttgcaagt ccattcatta aaagcgacta aaataatatc
     4261 ctgaattgga agtttaggac ttgcttcata ttcttgcgcc agagattcat aaggtgcaaa
     4321 attcaacaac caaaaataaa gattggcagc atcgctttca gtggttataa aagtggcttt
     4381 cagcattagg tccttatcca tacccaaaat agcttcagct ttgatgagcg gataatgctg
     4441 catttgccaa acggcttccc gaaaatcgct ctccaactta tttttaagtg aaggatttac
     4501 ccgattgtaa aacctgcgtg cttttgcggt tctaccaatg atattgccgt gacaatcgtt
     4561 aataattttg gcatctttcg gaagattatg cagttttacg aattccgggt acatcggtaa
     4621 atcagtaaca cttacaccgg gttgttttat tgccaattca tatgcctcgc gaatatctac
     4681 tttgcgaact agatggttat tcatcagagt ttcggcaatc gctctgatag gagacatttc
     4741 tttaatattg gaatagtatt ccacactact cttgcttgcc attttgatcc tccatatatg
     4801 gcttatttta ttttaaaaat atacgctgat tctcatttat atattaaatt atattaagct
     4861 ctttaccaat cattctgaat ttatccagag ccatatcgag ttgatcttgg gtatgagttg
     4921 ccataaaaga tgttctaatt aaacaggaac tgggtggaac agcaggagga actacgggat
     4981 taataaaaac accttcttca cctaaacgac gccacatatt aaaggcagtc atcatattgc
     5041 caacatgtaa aggaactact ggtgtgcagc tatctcctgt attatacccc attgctttaa
     5101 attctgataa catatagttt gtgttttccc atagcttggc tatgcgttca ggttcttctt
     5161 ccataatttt taatgctgct aaaacgctgg cagttgatgc aggtggaaga gatgcagaaa
     5221 aaataagagc tctagattta tgtttcaaat agtggataag agcttcatcg gcagctataa
     5281 aaccacctac tgatgctaaa gatttactga aagttcccat tatgaaagca gtcttatcag
     5341 ttaagccaaa atgagcagtt gttcctgcac ccaaatttcc taagacacct aaagaatggg
     5401 cttcgtcaac cataacctca gaattgtatt tttctgctaa atcacagata gcaggaagat
     5461 ttgcaatatc cccttccatg gaaaatatgc cgtccactac aattaaagaa ttcttgcttc
     5521 caatatcttt tagtaaatgt tccaaagaag ccatatcgtt atgtttatat cgcagcattt
     5581 tcccttcagc aagtaaagca ccatcaataa ttgaggcatg gtcatattta tctgttatca
     5641 caaaatcgtt cttcccaacc atagtagaaa tacatccaac atttgcttga taaccagtag
     5701 gataacaaat tgctgcttct ttgcctacta attttgccaa ttcgttttcc agctgcaggt
     5761 gaatatctaa agtgccattt aaaaaacgac tgccagcaca accgcttccg tatttttgaa
     5821 ttgcttttat ggcagcttct tttacgagag ggtgattcgt taaacctaaa tagctatttg
     5881 aacccatcat taacattttt tttccgttgc agataacttc tgtatcctgt tcggaagtaa
     5941 tttctcgaaa ataaggataa aagcccattg ccataacctt acgggcatct gtaaacttat
     6001 aacatttatc gagtaaactc attattacct cttttatatt attttaaagt ctttaagatt
     6061 tacatataag accttaatta gattataggt tatatataca agggggacag aaataagcat
     6121 ccaaagaatg ccaccatacc aaccacctgc aattaaagtt aacaaaacta atagtgggtg
     6181 cattttaatt gtgcctccta caacaacagg gtaaacgata ttattatcta tcaattggac
     6241 aatgtacatt gctattatta catatattgc atataggatg gagctaccat taatcaaaac
     6301 tgttgctact gctaatgctc ctcccaaaaa aggtccaaaa taaggtatga tattggcaac
     6361 tccagctgta gccccaatta atacagaatt tggaactccc acaattgtaa gagcagtaga
     6421 agctaaaata ccaacggcaa taatttcaaa caagatagct cgaaaaaaat tacctatagt
     6481 gctatcaatt ttacggaaca taattatgca aagctcaaaa tagcggttgt taaataaact
     6541 taaaatacct ttccggagtt tgtatttatc ctttaaaata aagaaactca ttaaaggaac
     6601 tgtagcaata atggatatag tggaaaatat ccttttataa ttatcaatca gcaatttggg
     6661 aattttcgct aaaaaaacat tggtctgatc taagatttct atcatcttgg gtgctatccc
     6721 taaaccaggt attttagtat ccaattcgac caaaaattca tatattctac taataaaagg
     6781 cagagttata atatattgcc cgtttagttg ttctccactt gccaaaatat taaaaagatt
     6841 gtttccttgt tgaactaaag ctggaaccaa acgggaagtg aaccaggcaa taattcccgc
     6901 gataataaga tagatagtta taactgctaa ccaacggggg aaatgatgat actcaagcca
     6961 gtttacaaat ggatcaagta tataagaaaa gattaaggca tatacaaggt agtatattat
     7021 cgaatgataa aaaaccacag caagaatgaa gattgcaaat aacaaaagat aaaaaatcag
     7081 ctttgtccag ttcattactc gatttttgcc tttatagctg aaattgttaa gatataatta
     7141 cttaaggttt gacaaaaagc atatagtaat ttaactccta aatgcggatt ctgctttgct
     7201 aaatccaata aatcaaaacg agaaatcaca taagtggtta catctgttat tgctttagct
     7261 gaactgctgc gagtttcttc gctgaacata tcaatcaaac ctaaaacctg attttttcca
     7321 ataacatagc aaccttgcgg gtgggtggga cctgttactt ctatttctcc cgataataca
     7381 aaataaataa cctctaaagg ataacctttg tcatatagaa tttctccagc ctgataggtg
     7441 cgcttatgca aaaaattact aagtaaatat agctcaaatg aactgcactc attgaaaaca
     7501 ggaaacagct ttaagtcggc atatttatcc ggttttttca ttatagtttt aagccaggcg
     7561 aaaacatctt ttaacatctt ttaccccaag ttgttattat cacaaacaag aggaacaaaa
     7621 gaacatcctc ctgcctgagt tatagtataa ccatcttcgg ttttgtttac tatatgtaat
     7681 atttgaaaac ctgctcttcc aattggaatt gctaacttac cacctataac taattgttcc
     7741 agtaaacggt ttgggatatc ttgcgctgcg gcagaaacaa ttatcttatc aaattcttta
     7801 tgaggtggat atgctttttg ccaaccgcta tgtccatccc caatgcgaaa ataaatattg
     7861 ttgtaaccgg cattgcgtaa aaccttttga gcattcaaag aaagcgattc tagtcgttct
     7921 acagtgcaaa cttctttaac aatttcagct agtaaggcac tttgataacc actgccggtt
     7981 ccaatctcca aaactgtatc tgatgatttt aaatctaata attgaagcat tatagctatc
     8041 atcaatggtt gcgaaatggt ttgtccctct tttatcggta aaggacgatt ctgatatgag
     8101 tattcacgat attctggtaa aacaaaattc tctcgtggca ctttagcaaa agcatctaac
     8161 actgccggag aagttattcc ttccagttta agctcgttta ctaattttaa tcgttgatcc
     8221 tcaaaccgca ttatcagctc tccaattgca gcaacttatt ttcttccagc caattaagaa
     8281 ttctgggata ggtatcactt tttgtaagtt caaagcccaa aggagttaaa gatatataac
     8341 cattatttac agcttccgca tcggatcctg tttgattatc ccaaactgga atatcaccgc
     8401 caacttcata aacaaaacca tcatcgtttt cttttataac cttaacaaaa ttataatact
     8461 tacgatgacc tgtccgggtt aaacgtatac cctttatttc ttctatagga agattaggaa
     8521 cattaatatt caaaactcca tattcttcag cttgtttatc gatgcctaaa tctaacaatt
     8581 tacgcatcca gtgagctgcc acatcgaatc tttggttttt ataagaagtt atagaaatag
     8641 ctattgccct atttcccaaa agagctgctt ccattgcggc tccaactgtt ccagaatata
     8701 aaacatcttc tcccatattc tgtcccgcat taattcccga aataatta
//
LOCUS       DAOSTH010000066         8572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_7845_length_8572_cov_12.702008, whole genome shotgun sequence.
ACCESSION   DAOSTH010000066 DAOSTH010000000
VERSION     DAOSTH010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8572
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7845_length_8572_cov_12.702008"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            117..470
                     /locus_tag="PLB65_06840"
     CDS             117..470
                     /locus_tag="PLB65_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOQ78280.1"
                     /translation="MKTLTCLCKSISEEEMDDIKKHIPKDNTIQNMAEFFKVFGDSTR
                     LKILYYLSRHELCVADLALLVNMQQSAISHQLKLLRLNRLVKYRKEGTTNYYSLDDEH
                     INSIFKVALEHLQEL"
     gene            471..2534
                     /locus_tag="PLB65_06845"
     CDS             471..2534
                     /locus_tag="PLB65_06845"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOQ78281.1"
                     /translation="MIIEEYDVHNLDCPDCGSQIEKEINELKEVERANLDFVNKRLTI
                     EYIEQVNNPLKRLNAIASKIDPDVQFTLAGLELEQTEKKPWFLILAILILTISLFFSK
                     PISTILGFVAYLIAGHKVIYKAFREICSRRIFTEHFLMTLATLGALYLGEFLEAAAVM
                     ILYEIGNYLESGAIAKSRRNLKSLLSLKPETAHLKTENGINELKLNEIKKGQVILVYP
                     GERIPLDGVVIKGESTVDTSSITGEAIPVFVESKSKVFSGFLNGEGMLEIKVQNTEAE
                     SMVTRIIKLIENASSEKSETEKFMTRFARYYTPAVVLAAVFVFLIPTILGFNYTVWLR
                     RALIFLVVSCPCALVISIPLTYYIGIGKAAKKGIIFKGSVYLDLLSKVKTLIFDKTGT
                     LTTGELRIAEIEAAPNIDPEELKRSTWLCEYTSSHPFAKAIKNAFSYNFVPSAVEAFS
                     EYPGKGVLLVYNKDRYLCGSENFLQQFGFVSLLDLGDNSVVHTVKNDMYLGCISFVDE
                     LKPGMKEVLQSLKQKGIKHLSVLSGDRYPKVERVANELGLDSYYAELIPESKLEKMEE
                     FLKNSIDKVAYCGDGLNDAPVLARADVGIAMGKIGAQASIETADIVLLNDKPEQLESA
                     FSISRETNRMVWQNIVLALTIKLLVMTLGAAGLAKLWEAIIADVGVTLLVIFNSLRMM
                     KKPKC"
     gene            2660..4561
                     /gene="thrS"
                     /locus_tag="PLB65_06850"
     CDS             2660..4561
                     /gene="thrS"
                     /locus_tag="PLB65_06850"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HOQ78282.1"
                     /translation="MIKVTLPDGSIREYEKPVKALQIAQDFDPELAEKSLCAEVNDKL
                     VDITYTIDEDAKINFPTFETDEGKEVYWHSTAHLMAQAVKQLFPSTKLAIGPAIEQGF
                     YYDFDREESFTDEDLQKIEMKMKELIAQSLPYHRTELSKAEAIELFSEMNETYKLELL
                     EDIPEDEIISSYKQGDFIDLCRGPHLTDTGRIKAIKLLRTSGAYWRGDEKNKMLKRIY
                     GISFPTEKELADYLQFLNEAMQRDHRKLGKDLDLFSINDEVGPGLVLWHPKGAMVRHL
                     IEAYWKEQHLKKGYQLVYTPHIGRASLWETSGHLGFYKENMYSKMDIEGQDYYIKPMN
                     CPFHISIYNSSLHSYRELPIRLAELGTVYRYERSGVLHGLMRVRGFTQDDAHIICTPE
                     QMDSEVEKLIVFSLEMLKAFGFKEFLIYLSTQPEDCVGKKEDWDKATASLAASLEKLG
                     LEYKVDEGGGAFYGPKIDIKIKDALGRAWQCTTIQFDFNEPERFDMTYIGADNTAHRP
                     YMIHRAVLGSVERFFATLLEYHSGNLPLWLAPVQIILIPITDAQIEYTRKIEEQLLEQ
                     GFRCEFDARSEKMGYKIRAAEKSKIPFMCIIGKNEEANNQVTLRQHTVGDLGAMNLEE
                     AISILRQKL"
     gene            4558..5526
                     /locus_tag="PLB65_06855"
     CDS             4558..5526
                     /locus_tag="PLB65_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOQ78283.1"
                     /translation="MNIYETVQEELAKEAFAIQIVAKQLSKESLEKAFELLCNCQGKV
                     VLTGMGKTGIIARKISATLASTGTTSIFLHSAEGIHGDLGMIESDDVVIAVSNSGNTQ
                     ELINLIPFLKFNRVPIIAITGEPNSQLAKNSDVVLNCNVPKELEPLGLVPTSSTTVAL
                     AVGDALAIALLKHKNFQLQDFARFHPGGTIGKKLLLRVINLMHSGDELPVIKDNATMS
                     EVIIEMTSKKLGCTAVTDKNGKLIGMITDGDLRRQLHNKGNSLLDYSAKDCMTDNPKT
                     LKPEVLAIEALNLMETYKITMIPVVDGSNQPVGMLHMHDLIAAGVI"
     gene            complement(5523..7241)
                     /locus_tag="PLB65_06860"
     CDS             complement(5523..7241)
                     /locus_tag="PLB65_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HOQ78284.1"
                     /translation="MNLLQIINLVGGLALFIFGINKMSDSLQAVAGSEMRRILKVLIN
                     TPLKGVLVGIGVTALIQSSSATTVMMIGLVNTGIMTLQQAVGVVMGANIGTTITAQLI
                     AFDLGQYAFVFIILGVTLIFIRKSRAMEHWSQIIFGFGLLFLGLNVMAESVAPLQNSV
                     VARNLMIKLASNPIMSILVGTGFTMLIQSSAASIGIVMVLASTGLIPFEGALYLVFGD
                     NIGTTITAWLAAIGSSNTAKRVAMVHTLFNVFGTIIFGLLTYLGLYPLFVNWITPGNV
                     YAGQNIARHIANGHTMFNIVNTIVFIPFAGQLAKIATKIIPDDKVETISLGEPKHLNY
                     TIINNSYLAINQSIKEMREMLRLVRMELLVTYQAFKEKDYKKHMRVSRIEKAIDNLQK
                     EITLYLVAVNEKTNAPDIIHKIPALLHTVNDIEKIGDFTEEMNNILNEQIITQKHQLP
                     LEFTAMIDDLHSKLLYMLDLSIDYLVDLKEDYIYKIIEMEGRINESHYRLREEILSKI
                     QNAVCDAEGGLNTIDYLDEAEEIADKLKNLVKAGSHNFIYNHNYFKELNNQDNQEEAV
                     EENEVF"
     gene            7424..7693
                     /locus_tag="PLB65_06865"
     CDS             7424..7693
                     /locus_tag="PLB65_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOQ78285.1"
                     /translation="MPTWEIYVNGRVQGVGFRYFVWKAATELGIKGYVQNQWDGKVKI
                     VAQAEEHTLHTFQIIVAQGNRYAFVKELKINKLDSAEKYNDFEIR"
     gene            7674..>8572
                     /locus_tag="PLB65_06870"
     CDS             7674..>8572
                     /locus_tag="PLB65_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HOQ78286.1"
                     /translation="MISKLDKYLWLMLMQIMFILPLGLSAVDQIHTVKKGDTLYSLSK
                     RYGTTIDELKKLNSISGNNLSIGQKLIIKKDVPKPKPVTPAPAIKPIPTEIPEGSSPP
                     PISGASTTENISLPTIPITIPSEYYYTVKAGDNLYRIAVNNNITLKELLDWNNFANSS
                     VRIYPGDKLLIKNPSEVSETKIAPVEAVKDPVASINPTPTEEDTILIERVYIVQKKDT
                     LYRIATNNGMTVEELMQLNNLTSTNLYVGQKLYLSGKPRLVPSTPPEILTEEELMKRD
                     KIRTDLIMPIEGHIISEYGLRNGR"
BASE COUNT         2843 a         1454 c         1610 g         2665 t
ORIGIN      
        1 gactctttat attaggaaag attagttgta aatcgagcga tgaaatatac caattttttt
       61 cttgacaagc gtccctccct agtaatatat gaacatatat tcataaagga gctgaaatga
      121 aaacgctaac ttgtttatgc aaaagcattt ccgaagaaga aatggatgat atcaaaaaac
      181 atatccccaa agataatact atccaaaata tggccgaatt ttttaaggtt tttggggata
      241 gtactcgcct gaagatttta tattaccttt cgcgtcatga attatgtgtt gccgatttag
      301 cgttacttgt aaatatgcaa caatccgcta tttcgcatca gctgaaactt ctccgtttaa
      361 accgtttggt gaaatatcgt aaagagggca ctactaatta ttattctctt gatgatgaac
      421 atatcaacag tattttcaaa gttgcactgg aacatttaca ggagctttaa atgataatcg
      481 aagaatatga tgttcataat ttggattgtc ctgattgcgg ttcccaaata gaaaaggaaa
      541 taaacgaatt gaaagaagtg gaaagagcca atcttgattt tgtgaataag cgtcttacca
      601 tagagtacat agagcaagtg aataatcctc taaaacgatt gaatgccata gccagtaaga
      661 ttgatcccga tgtacaattt actttagcag ggttggagct ggagcaaacg gaaaaaaagc
      721 cctggttttt aatcttggcg atattaattc ttacaatcag cttatttttc agtaaaccca
      781 tctccactat ccttggtttt gttgcttatc taattgctgg tcataaagtt atttataaag
      841 cattcagaga aatctgctct cgcagaattt ttactgaaca ctttctgatg accttggcaa
      901 cattgggagc attatattta ggggaattcc tggaagcagc agctgttatg attctgtatg
      961 aaataggtaa ttatttggaa agtggagcga tagccaaaag ccgtagaaat ttaaaatctc
     1021 ttctttctct aaaaccagaa acagcacact taaaaaccga aaatggcata aatgagctta
     1081 agcttaacga aattaaaaaa gggcaagtga tacttgttta tccaggggag cgaattcctt
     1141 tggatggagt tgttataaaa ggagaatcca ctgttgatac ttcttccata acgggagaag
     1201 caattcctgt gtttgtagaa agcaagtcca aggttttttc tggatttcta aatggtgaag
     1261 gaatgctgga gataaaagtt cagaatacag aagcggaaag tatggttact cgcattataa
     1321 agctcattga aaacgccagc agtgaaaaat ctgaaacaga aaagtttatg acccgttttg
     1381 cccgatatta cactccggca gttgtattgg ctgcagtatt tgttttttta attcccacta
     1441 tattaggttt taattacact gtatggctac ggcgcgcttt aatttttctg gtagtttcct
     1501 gcccttgtgc tttagtaata tccatacctc taacttatta tattggaata ggtaaagcag
     1561 caaaaaaagg tatcattttt aagggtagcg tgtatcttga tctacttagt aaagtaaaaa
     1621 ccctgatatt tgataaaacc ggaactctta caaccggtga attgcgtatt gcagaaattg
     1681 aagcagcccc gaatattgat ccagaagaac ttaaacgcag cacatggctt tgtgaatata
     1741 cttcttctca tccttttgca aaagcgataa aaaatgcttt tagttacaat tttgttcctt
     1801 ctgccgttga agctttttct gaatatccag gcaagggagt gcttctggtt tataataaag
     1861 accgttatct ttgtggaagt gagaattttt tacagcaatt tggatttgtt agtttactcg
     1921 atttgggtga taatagcgtt gtacataccg ttaaaaatga tatgtatctg ggttgtatca
     1981 gtttcgtgga cgagctgaaa ccaggaatga aagaagttct gcaatcgctt aaacaaaaag
     2041 gtattaaaca tttaagtgtt ctttctggag accgctatcc taaagtagaa agagtggcaa
     2101 atgaacttgg tttagatagt tactatgctg aattaattcc ggaaagtaaa ctggaaaaga
     2161 tggaagaatt tttaaaaaac agcatagata aagttgccta ttgcggagat ggtttaaatg
     2221 atgctcctgt tttggcaaga gctgatgtag gaattgcaat gggaaaaatt ggagcgcaag
     2281 catcaatcga aacagcagat attgttttat tgaacgataa acccgaacaa ctggagagcg
     2341 ctttcagcat ttctagggaa actaatagga tggtttggca gaatatagtt ctagctttaa
     2401 ccatcaaatt actggttatg actttaggag ctgccggact ggccaaactc tgggaagcga
     2461 taattgccga tgttggagta actttactgg ttatcttcaa ttcacttaga atgatgaaaa
     2521 agccgaagtg ctaatactct tatatatctt ttaaggtagt ttctattatc ctggatttaa
     2581 aatcattctc ggcttgacag aataatattt atagaaaaat aggttatttt tttaaaagaa
     2641 tattgaaatg gagttataaa tgataaaagt aactttacct gatggtagta tccgagaata
     2701 tgaaaaacct gtaaaagctt tacaaattgc tcaagatttt gatcccgagc ttgctgaaaa
     2761 atcactatgt gcagaagtga atgataagtt ggtagatatt acatacacaa tagatgaaga
     2821 tgctaaaata aacttcccca cttttgaaac cgacgaaggt aaagaagtat attggcatag
     2881 tactgctcat ttgatggcac aggcagtaaa gcaattgttc ccttcaacaa aacttgcgat
     2941 tggaccagct attgaacagg gtttttatta tgattttgat agagaggaat cttttacaga
     3001 tgaagatttg caaaagattg aaatgaaaat gaaagaactt attgcccaat cccttcccta
     3061 tcataggaca gaattatcta aagccgaagc aatagaactt tttagcgaaa tgaatgaaac
     3121 atataaattg gaacttttag aagatatccc cgaagatgaa attatcagtt cttataaaca
     3181 aggtgatttt attgatcttt gccgtggacc acatcttacc gatacgggca gaattaaagc
     3241 cataaagtta ctaagaactt caggtgccta ctggcgtggt gatgaaaaaa acaaaatgct
     3301 gaaacgaatt tacggaatca gttttcctac agaaaaagaa cttgctgatt atctgcaatt
     3361 tttgaatgaa gctatgcagc gcgatcatag aaaattggga aaagaccttg atctattttc
     3421 gattaacgat gaagttggcc ccggacttgt attatggcat cctaaaggag caatggttcg
     3481 tcatttaatt gaagcatatt ggaaagaaca gcatctaaaa aaaggttatc aattagtgta
     3541 tactccgcat attggaagag cgagtttatg ggaaaccagt ggtcatcttg gtttctataa
     3601 agaaaatatg tatagtaaaa tggatattga aggtcaagat tattatatca aaccgatgaa
     3661 ttgtccgttt catatcagta tctacaattc ttctttacac agctatcggg aattacctat
     3721 tcgccttgca gaattaggaa cagtttacag atacgaacga agtggtgttt tacacggttt
     3781 aatgagggtt cgtggtttca ctcaagatga tgcacatata atttgtactc cagaacagat
     3841 ggatagtgaa gtggaaaaat tgattgtctt ttctttggag atgttaaaag cttttggctt
     3901 taaggaattc ctaatttatc tatctactca acctgaggat tgtgttggta aaaaagaaga
     3961 ctgggataaa gctactgctt ctttggctgc ttctttggaa aagcttggtt tagaatataa
     4021 agtagatgaa ggtggcggcg ctttttatgg tcccaaaatt gatattaaga taaaagatgc
     4081 tttgggtaga gcttggcaat gcactactat tcaatttgat tttaatgaac cagaaagatt
     4141 tgatatgaca tatatcggtg cagataatac tgcccataga ccttatatga ttcatagagc
     4201 tgttttaggt tctgtggaaa gattttttgc tactctactg gaatatcaca gtgggaattt
     4261 acccctttgg cttgctcccg tacaaataat actaattcct ataaccgatg ctcaaataga
     4321 atatacccgt aaaattgaag aacaattact ggaacaaggt ttccgttgtg aatttgacgc
     4381 tcgcagcgaa aaaatgggat acaaaattcg cgccgcggaa aagagtaaaa tcccctttat
     4441 gtgtattatc ggtaaaaatg aagaagccaa taatcaagtt actttacgtc aacatacagt
     4501 cggtgatctt ggtgctatga atttagaaga ggcaatctct attttgagac agaaactatg
     4561 aatatttatg aaacagttca ggaagaatta gccaaagagg cattcgctat acaaatagtg
     4621 gcaaaacagc ttagtaaaga atctctggaa aaagcctttg aactgctttg caattgccaa
     4681 ggaaaagttg ttttaaccgg aatgggaaaa acagggatta ttgccagaaa aataagtgct
     4741 accttggctt ctacaggaac cacttctatt ttcttacatt ctgcggaagg tattcatggc
     4801 gatttaggaa tgatagaatc tgatgatgtg gttattgctg tttccaatag cggaaacaca
     4861 caagaactta ttaatctcat tccctttctg aaatttaatc gggttccaat tatagctatt
     4921 acaggtgaac caaattcaca attggcaaaa aattctgatg tagtcctcaa ttgtaatgtc
     4981 cccaaagaac tcgaaccctt aggtttggtt ccaacttctt ctactaccgt agctttggca
     5041 gtgggagatg ctttggctat tgctttacta aaacataaga acttccagtt acaagatttt
     5101 gcccggtttc atccaggtgg gacaattggt aaaaaattgc ttttacgagt gatcaattta
     5161 atgcatagtg gtgatgagct tcccgtgatt aaagataatg ctacgatgtc tgaagtaata
     5221 attgaaatga cttctaaaaa acttggttgt acagctgtaa ctgataaaaa tggaaaactg
     5281 attggaatga taaccgatgg tgatttaaga cggcaactgc ataataaagg taattctctt
     5341 ttagactatt cagctaaaga ttgtatgacg gataacccca aaacgctaaa accggaagtt
     5401 ttagctattg aagccttaaa tttgatggaa acatataaga taacaatgat ccctgttgtg
     5461 gatggtagta accagccggt cggtatgtta catatgcacg atttgatagc agctggcgtt
     5521 atttagaata cctcgttttc ctctactgct tcttcttggt tgtcctgatt attgagttct
     5581 ttaaaataat tatggttata gataaaattg tggcttcctg ccttcactaa gtttttcaat
     5641 ttatcagcaa tttcctctgc ctcatcaaga taatcaatag tgttcaatcc tccttcagca
     5701 tcacatactg cattttgaat tttggaaaga atctcttccc gtaaacgata gtgactttca
     5761 ttgatgcgtc cttccatctc gattatctta tatatataat cctctttcag atctacaaga
     5821 tagtctatgg ataaatccaa catataaagt aatttggaat gtaaatcatc tatcatagcc
     5881 gtaaactcaa gaggaagctg gtgtttttga gtgattatct gttcgttcag aatattattc
     5941 atctcttcag taaagtcacc tattttttca atatcgttta ctgtgtgcaa taaagcaggt
     6001 attttatgaa taatatctgg cgcattagtt ttttcattaa cagctaccag atatagcgta
     6061 atttcctttt gcaaattatc aattgctttc tcaattcggg agactcgcat atgcttcttg
     6121 tagtcctttt ctttgaatgc ttgataagta accaaaagct ccattctcac taaacgcagc
     6181 atttcccgca tttcttttat ggactgattt attgccaagt aggaattgtt tataattgtg
     6241 taatttaaat gtttgggctc gcctaaagag atagtctcca ctttatcatc agggataatt
     6301 ttagttgcaa tttttgccag ctgccccgcg aaaggtataa atacaatagt attaactata
     6361 ttaaacatag tatggccatt agcaatatga cgagcaatat tttgccctgc atacacattg
     6421 cctggtgtaa tccaattcac aaataaggga tataatccca gataagttaa taatccaaaa
     6481 atgattgttc caaacacatt gaataaggtg tgaaccattg ctactcgttt tgctgtatta
     6541 ctactgccta ttgctgccaa ccaagcagtt attgtggttc caatattatc tccaaaaact
     6601 aaatataaag caccttcaaa agggattaaa cccgtagagg ctaaaaccat cactattccg
     6661 atcgaggcag cagaactctg aattaacatt gtaaatccag ttcctaccaa tatactcata
     6721 ataggatttg aagctaattt aatcattaaa ttacgggcta caacagaatt ctgtagagga
     6781 gcaactgatt ccgccataac attcaaacct aaaaacaata agccaaaacc aaagataatt
     6841 tgactccaat gttccatagc tctacttttg cgaatgaaaa ttaaagtaac ccctaagatt
     6901 ataaacacaa aagcatattg accaagatca aaagcgatta attgagcagt tatggtggtt
     6961 ccaatattag ctcccataac tacacccact gcttgttgta aggtcataat tccagtatta
     7021 actaaaccaa tcatcataac agtggtagct gagctacttt gaattaatgc tgttacacct
     7081 attcctacta aaactccttt taagggagta ttaataagaa cttttaatat cctacgcatt
     7141 tcggaaccag caacagcctg taagctatcg ctcattttat tgattccgaa aataaatagt
     7201 gctaatccac ccactaagtt tatgatttgc agtaagttca tctagtccag aagttccttt
     7261 tttatatgtt aagttcttac tttttttcct cccacgatat ggtcaaggta tttttggttg
     7321 acaagaggtc aaattatacc ttcctgtaaa aataaagaaa acgaatcctt gacaaattca
     7381 agcagttaat ttatgcagaa caaataatct aaaggtttga tatatgccta cttgggaaat
     7441 ttatgtgaat ggtagagttc aaggagttgg tttccgatat tttgtttgga aagctgctac
     7501 cgaacttggc attaaaggtt atgttcaaaa ccaatgggat ggaaaggtca aaatagtcgc
     7561 ccaggcagaa gaacatactt tgcatacttt tcagattata gtagcccaag gcaatagata
     7621 tgcttttgta aaagagctaa aaattaataa attggatagt gcagaaaaat ataatgattt
     7681 cgaaattaga taaatatctt tggctgatgc tgatgcagat aatgtttatt ctgcccttag
     7741 ggttatcagc tgtagatcaa attcataccg ttaaaaaagg cgatacgcta tactcgctta
     7801 gcaagcgata tggcacaact atcgatgaac ttaagaaact gaatagcatt tctggtaata
     7861 acctttctat tggccaaaag ctgataatta aaaaagatgt tccaaaacct aagcctgtta
     7921 ctcctgctcc agctataaaa cctattccca cagaaattcc agaaggctct tccccacctc
     7981 caatttctgg tgcttcaaca accgaaaaca tttccctgcc aacaattcct ataaccatcc
     8041 cctctgaata ttattatact gttaaagcag gtgataatct gtataggata gctgtgaaca
     8101 ataatatcac tttgaaggaa ctgctagact ggaataactt tgctaatagt tcagtgcgga
     8161 tttatcctgg ggataaacta ctaattaaga atccaagtga agttagcgag acaaaaattg
     8221 caccagttga agcagtaaaa gatcctgtag catccattaa tcctacccct acagaggaag
     8281 atacaattct gattgaacga gtatatatag ttcaaaaaaa ggatacttta tataggattg
     8341 ccacaaataa cgggatgacg gttgaagaat taatgcaact gaataattta acctcaacca
     8401 atttatatgt gggacagaaa ttatatcttt cagggaaacc tcgtcttgta ccttctacgc
     8461 ctcccgaaat tcttacagaa gaagaattga tgaaaagaga taagatccgc accgatctaa
     8521 ttatgccgat tgagggacat attatttccg aatatggttt gcgaaatgga cg
//
LOCUS       DAOSTH010000067         8477 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_7933_length_8477_cov_13.266089, whole genome shotgun sequence.
ACCESSION   DAOSTH010000067 DAOSTH010000000
VERSION     DAOSTH010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8477)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8477)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8477
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7933_length_8477_cov_13.266089"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="PLB65_06875"
     CDS             complement(<1..494)
                     /locus_tag="PLB65_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HOQ78287.1"
                     /translation="MKTHNLTLPLSPAEIDKLKINDKVLLTGYLFTARDVAHKRMMEI
                     IQKGEVLPFELSEIALFYCGPSPVRPGNICGAIGPTTSSRMNSYTPYFIQKGLRVMIG
                     KGEISSEVQNTIKEYKAVYLTMAGGISAYLSQFIVSCETFLWPELGPEAIHKLLVKEI
                     PAYV"
     gene            complement(498..1349)
                     /locus_tag="PLB65_06880"
     CDS             complement(498..1349)
                     /locus_tag="PLB65_06880"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOQ78288.1"
                     /translation="MNMRYVSRETIEEELTKAINYITFNPDPELPALLKSALADETND
                     VSKDILNCMLENIKLAPKIEIPLCQDTGSFIVFAEIGNQCSIPDAPLSQIINETLIKV
                     SAELYLRPSILKDPLLHRINTGTNAPAVIHISIVEGDKLILHIAQKGGGAENMSQLKM
                     FTPSANPEDIIDFVIHTVQVAKGNACPPLIIGLGIGGNFETCAILAKKALLAPISHKN
                     PVLEYAALEQNILKSVNRTGIGVQGMGGKNTALAVHILTAPCHIASLPVAVNLQCHSH
                     RHTTIII"
     gene            complement(1353..2231)
                     /locus_tag="PLB65_06885"
     CDS             complement(1353..2231)
                     /locus_tag="PLB65_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOQ78289.1"
                     /translation="MPLLSEETLARLNKFQLMAKSIVEGFLVGLHKSPYHGFSAEFSD
                     HRQYNPGEPLKDLDWKILAKTERYYIKRYEEETNLRSYVLLDHSKSMFFSSGKESKIE
                     YATQLAGALIWLMISQKDAAGLYTFNNKITSAYPPKAIRSYTSQLFTALINLKADDKT
                     DLLTPLHQIAELIKKRSLIILISDLLDDPEKIMESLKHFRARNNEVLVFHIVDPKEEK
                     FDYKREAQFIDSETGEKITVSPWQIRKEYQENYHYFTDYLKKECYKHQIEYNPVDTYT
                     PIEDLLLKYLIKRNKG"
     gene            complement(2233..2433)
                     /locus_tag="PLB65_06890"
     CDS             complement(2233..2433)
                     /locus_tag="PLB65_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78290.1"
                     /translation="MKPEKKDNDKLPLGRINFIILLVAALLLIAGYFIMSFNEISISP
                     ILLVVAYAIVIPLALLYQPKKK"
     gene            complement(2503..3288)
                     /locus_tag="PLB65_06895"
     CDS             complement(2503..3288)
                     /locus_tag="PLB65_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOQ78291.1"
                     /translation="MIIKKVHCILWHIWLMSDFFITAAIIRITNRNKIKRRQRYLRNN
                     ARISKKFLKAFNIKLNVKHIENLKQLHKLPYLAVSNHTTYLDIIILASLENYIFITSV
                     EMRGIPFLGKITRNGGCLYTNRKKYVSLPEEINRFTAALLQGFKVMLFPEGTSTNGIT
                     VQPFHKSLFQIAIKAKCPVLPICIKYLTLDGKKIQNENRDRIAWYGKMNFLLHYLKLV
                     GHKLEAEVEILEATPYQEGKTRQDISDDAYQKILKCYQQEKFS"
     gene            complement(3413..6136)
                     /locus_tag="PLB65_06900"
     CDS             complement(3413..6136)
                     /locus_tag="PLB65_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ78292.1"
                     /translation="MKRYLIYALLFFPALILANSYSVQIQFPFPREGFSSSALYEQGY
                     GFYIAPGALQLPVKQINILLPKDAIVNDWQLTFDPDITFKGEAPIFNSAFTNGEEILG
                     APIKRNNPSRYNFLGLRKWGDLNYASFYVIPGIYDGSNWLWNSSCRIDINYSASAKQN
                     GKIPPTFQKRDFFANPQCLINWYEASKEPNNRLLVITTPSLYTALNDLVLFRQTQGID
                     VNFCDIATALASGTGTDNAEKLRNYLQNTYNQNPFSYLLLVGDDVTIPTAYVTPEPNG
                     LETVATDFFYSDLSSNWDTDSDGLRGEYSTGYLNEDYGIDFTPEVFVGRISTNYASEV
                     SAIANRIVAYEQSTEPWKDKNLLSAAFLNYQGEPEIIYPQTDGGLFMEFLRNTCLVGQ
                     DNFTMYEQEGVIPSFPSDLPANYNNLLNALNNESWGFINWSAHGSAIHSSRKVWSIDY
                     NNNNIPDTYEMNWMPLVDCQSFDDLENQDGTVIFAASCYNGYMDYYDTCLSEYALIKK
                     AVGVLAATRTGWYKIGWLNPGWGGLSSYNYHFVENFRNNKLDLGSAHSWANLLHTQYY
                     LFGDPIDSEGIIYPELQNVYTYMLFGDPMIGWALEETDLDGEILVWEPEGNAGLEVVN
                     ALREIRNFNVIYTDKLIPEYSYLHNFEAVIYLAANSVASSSLDPDSYEYEYLNSYLDS
                     GGKLYCENYIDFSFGELYNKMGAQLDNPVNLNTIYHPESNISWNYSNADSTVYALIPI
                     QPNAQGIFISGDGETDNSIVGILNSTEDYSVITSSFQLSRILPGEHNLPDLMEIILAE
                     LNVTNSNPDSNNEQIICPTVQSVSVYPNPASQNSQLHFQMRKATPISISIFNIKGQKV
                     KTLINNELKEGEYHLSWDGKDNNGRTCSSGVYYYQIAAPNQNITKKMLLLK"
     gene            complement(6169..6927)
                     /locus_tag="PLB65_06905"
     CDS             complement(6169..6927)
                     /locus_tag="PLB65_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOQ78293.1"
                     /translation="MNYNYDHSRIVVILVEPIYGGNVGAIARIMNNFCFSQLRIIGKT
                     PEKNDFYLAMHSEHILENVCIYSSLAEAVKDLDRVIAFSRRIGKNKPVDLSPRQMARY
                     VHSVPNAKIGLVFGRETYGLTDSEAELCPLRCHFMANPDFPSINLAQAVALALWEIYS
                     LPLEEKQGENKYYNAASKEELDEIHHYIIQVMHSTGFFQKQETVNWDAFIAKMLNELN
                     PDKTMLYRLRQIFNRFNVLVTGKGLGYSISNDKE"
     gene            complement(6930..7814)
                     /locus_tag="PLB65_06910"
     CDS             complement(6930..7814)
                     /locus_tag="PLB65_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HOQ78294.1"
                     /translation="MIKEVGRLNLDDYKILDAEVEKIFRNLDKGKSALAEKQIIDIGN
                     TANYFVREELGKRLAQYSGKGELDDICGRLLENFIYGVRATALFYFYYKHQEHPERII
                     NTLDKTFETVPWESETICFELWKKDPEVMQEYMPLWAESDNEKKRAISMHGMENIAYR
                     HPQYVLSFVSRLLDDESEEVQKKVSHILTQVGRQRPVQCYNNIRHWLIEGDEKRIKTL
                     WQTMKKLANIYSQRSRRDKTNEFMNVTQKTIADWRNDSNPKVSMMGSKLSGIINKKRK
                     KTQQNQKVANSGNNHPSR"
BASE COUNT         2447 a         1587 c         1591 g         2852 t
ORIGIN      
        1 tggacataag ctggtatctc tttcaccaag agtttatgaa tagcttcggg gcccaattct
       61 ggccagagga atgtttcaca tgaaacaata aattgggata gatatgctga aataccccct
      121 gccatcgtta aatagacagc tttatattct ttaattgtat tttgaacttc actggatatt
      181 tctcctttgc caatcatcac tcttagtcct ttttgaataa aatacggagt gtagctattc
      241 attcgagaac tggtagttgg tcctatagcg ccacaaatat tacctggtct tacaggactt
      301 ggtccacaat aaaatagtgc tatttctgat agttcgaagg ggagcacttc ccctttttgg
      361 ataatctcca tcattctttt atgggcaaca tctctggcgg taaacaaata tccagttaat
      421 agcactttat cgtttatctt caatttatct atttcagcag ggctaagcgg cagggtaaga
      481 ttatgtgttt tcatctttta gataattatt gtagtatgcc gatgtgaatg gcattgtaaa
      541 tttacagcta ctggcaagga ggcaatatga cagggagcag ttaaaatatg gacagccaag
      601 gcagtgtttt ttcctcccat accttgcact ccaattcctg ttctattcac agattttagg
      661 atattctgtt ctaaggcagc atattccaaa actggatttt tatgggaaat gggagcgagt
      721 aatgcttttt tagctaagat agcacaagtt tcgaaattac ctccgatacc caatccaatt
      781 attaagggag ggcaggcatt tcctttggcc acttgaactg tgtgtattac gaaatcaata
      841 atatcttcgg ggttagcgga aggagtaaac attttcagtt gactcatatt ttcggcacct
      901 cctccttttt gagcaatatg caatattaat ttatcacctt ctacaataga gatatggata
      961 acagccgggg cattagttcc ggtatttatt ctatgtaaga gaggatcttt taggatagaa
     1021 ggacgcagat agagttctgc agagacttta attaaggtct cattaataat ttgggataag
     1081 ggtgcatcag gaatactgca ttgatttcca atttccgcaa aaactataaa agaaccggta
     1141 tcttggcaga gggggatttc gatttttggg gctaatttaa tgttttccag catacaatta
     1201 aggatatctt tactaacatc gtttgtttca tcagcgaggg cagattttag caaggcaggc
     1261 aattctggat caggattgaa ggttatgtaa ttaattgcct tagtgagttc ctcctcaatt
     1321 gtttcacgtg aaacatagcg catattcatc gcttatcctt tatttctttt aataaggtat
     1381 ttcagaagta gatcttcaat aggggtatag gtatctactg gattgtattc aatttggtgt
     1441 ttgtaacatt ccttctttag gtaatctgta aaataatggt aattttcttg atattccttg
     1501 cggatctgcc aaggggaaac ggttattttt tcacctgttt cactatcgat aaattgggct
     1561 tcccttttat aatcaaattt ctcttcttta ggatctacga tatgaaacac taaaacttca
     1621 ttattgcggg cgcggaagtg ttttaaggac tccataattt tttcaggatc atctaagagg
     1681 tcacttatta ggatgataag agagcgtttt tttatcaatt ccgctatttg atgtaaaggg
     1741 gttagcaggt ctgttttatc gtcagctttt aggtttataa gggcagtaaa cagctggctg
     1801 gtatagcttc taattgcttt gggcggataa gcagaagtta ttttattgtt gaaggtatat
     1861 agtccagcgg catctttttg ggaaatcatc agccagatta aagcacctgc cagttgagtt
     1921 gcatattcta ttttactttc tttgccagag ctaaaaaaca tagatttact atggtctaaa
     1981 agtacatagc tgcgcaggtt ggtttcttct tcgtaacgtt ttatataata gcgttccgtt
     2041 tttgccagaa ttttccaatc caggtctttt agaggttcac caggattata ctgtctatgg
     2101 tcagaaaatt cagcgctaaa gccatgatag ggggatttat gcagtccaac taaaaaaccc
     2161 tcaacgatgc ttttagccat cagctgaaat ttgtttaagc gtgctaatgt ttcctctgat
     2221 agtagtggca tttcatttct ttttaggttg gtaaagaaga gctaaaggaa ttactatcgc
     2281 ataggcaaca accaaaagga taggagaaat ggatatctca ttgaaggaca taataaagta
     2341 gccagcgata agcaataaag ctgccaccag cagaatgata aagttaatcc gacccagtgg
     2401 taatttgtca ttatcctttt tttcgggttt catatctgct ccttaaatta tagctttaat
     2461 atttcttgca tagagtcaat acttataaac ctgcgttgtt tgtcaactga acttttcttg
     2521 ttggtagcat tttagaattt tttgatatgc atcatcggat atatcctgtc tggtttttcc
     2581 ttcctgatag ggtgtagcct ctaagatttc aacttccgct tcaagtttgt gtcctactaa
     2641 tttgaggtaa tgtaacagga aattcatctt gccataccaa gcgattctat ccctgttttc
     2701 attttgtatt tttttaccat ccagggtgag gtatttaatg caaattggca ggaccggaca
     2761 ttttgccttt atggcaattt gaaataggga tttatgaaaa ggttgtacag taataccatt
     2821 tgtgcttgtt ccttcgggaa aaagcattac cttaaaaccc tgcaaaaggg cagctgtaaa
     2881 tctatttatt tcttcaggca gggaaacata tttcttgcgg tttgtgtata agcaaccgcc
     2941 attacgggtt atttttccta aaaaggggat tccccgcatt tctacagaag tgataaaaat
     3001 atagttttct aacgaagcca aaatgatgat atccagatag gttgtgtgat tggaaacggc
     3061 caaatagggt agcttatgta gttgcttcaa attctcaatg tgttttacat tcagtttgat
     3121 attaaaagcc ttgaggaact ttttactgat ccgagcattg tttctaagat agcgttgccg
     3181 gcgttttatc ttatttcggt tagtaattcg aattatggca gctgtgataa aaaaatcact
     3241 catcagccat atatgccaga gtatgcaatg tacttttttt attatcatag cttagttttc
     3301 catctggtct gcatcctatc cttaggcatt ttatctattt aaggttattt tatttgttca
     3361 tcctgggtat taatgaaact ctttatactc ccaaatctta atactccaag ctctatttta
     3421 gcagcagcat ttttttggta atattctgat tgggggcagc tatttgataa taataaactc
     3481 cactggaaca agttcttccg ttattatctt ttccatccca ggataaatgg tattcgcctt
     3541 cctttaattc attattaata agggttttta ccttttgtcc tttaatattg aagatagaaa
     3601 tggatattgg tgttgctttt ctcatttgaa agtgaagctg tgaattttgt gatgccggat
     3661 tgggataaac gcttactgat tgtactgttg gacagataat ctgttcgtta ttgctatcgg
     3721 gattactatt tgtaacattt agttcagcta gaataatctc cattaaatct ggtaaattat
     3781 gttcacctgg tagaatacga gataattgaa acgaagaggt aataaccgag taatcctctg
     3841 tcgaattcaa aatgccaaca atggaattat cggtttctcc gtctcctgat atgaatattc
     3901 cctgagcgtt aggttgaata ggtattaaag cgtaaacggt tgaatcggca ttgctataat
     3961 tccagctaat attgctttcg ggatggtaaa ttgtattaag attaactgga ttatccagtt
     4021 gagcgcccat tttattatat agttctccaa aactaaaatc tatatagttt tcgcagtata
     4081 gtttaccgcc agaatctagg tagctattta gatattcgta ttcataggaa tcgggatcga
     4141 gggaagaact ggcaacggaa ttagctgcca gatagataac agcttcaaaa ttgtgcagat
     4201 agctatattc agggatgagt ttatcggtgt aaattacatt gaaatttcta atttctctta
     4261 gtgcattaac aacttccaat cctgcatttc cttctggttc ccacaccaaa atttcaccat
     4321 ccaaatctgt ttcttctaaa gcccacccaa tcatcggatc gccaaaaagc atataggtat
     4381 atacattttg caattcggga tagattatcc cttcgctatc tataggatca ccaaaaagat
     4441 aatattgagt atgcagaagg tttgcccaag aatgtgcaga accaagatca agcttgttat
     4501 tacgaaagtt ttctacaaaa tgatagttat aactggaaag tccgccccaa ccaggattca
     4561 gccatcctat tttataccat ccggttctgg tggcagctaa aacacccaca gcttttttta
     4621 ttaaggcata ttcggacaag caggtatcat aataatccat atatccgttg taacaggaag
     4681 cggcaaatat aacggttcca tcctggtttt ccaaatcatc gaaactttgg caatctacca
     4741 agggcatcca attcatttca taggtatctg gaatgttgtt gttattatag tctatcgacc
     4801 atacttttcg ggaagaatgg attgcgctac cgtgtgcgct ccaatttata aagccccagc
     4861 tttcattatt aagtgcattt agaagattgt tataatttgc aggtaagtca ctgggaaatg
     4921 atggtattac accttcttgc tcatacattg tgaaattgtc ttgtccaacc aaacaggtgt
     4981 tgcgtagaaa ttccataaaa agtccgccat cggtctgtgg ataaattatc tctggttcgc
     5041 cttgatagtt taagaaggca gcagatagca gatttttatc tttccaaggc tcggtagatt
     5101 gttcatatgc aacaatgcga ttggcaatag cagatacctc tgaagcataa ttagtagaaa
     5161 ttctccctac aaatacttcg ggagtaaaat caataccata gtcctcattc aaataaccag
     5221 tagaatattc ccccctcaaa ccatcgctat cggtatccca attactgctg aggtctgaat
     5281 aaaagaaatc tgttgcaaca gtttccaatc cattgggttc gggggttaca taagcagtag
     5341 gaattgttac atcatctcca actaatagca aataggaaaa aggattctga ttataggtat
     5401 tttgcagata gttgcgtaat ttttcagcat tatcagttcc cgttcccgaa gccaaagctg
     5461 ttgctatatc acaaaaatta acatcaattc cttgtgtctg gcgaaataaa acaagatcat
     5521 tcaaagcagt ataaagcgag ggagttgtaa taactaaaag acggttattc ggttccttag
     5581 aggcttcata ccagtttatg agacattggg gattggcaaa gaaatccctt ttttggaaag
     5641 tgggtggaat tttcccattt tgtttggcag aagcagagta gtttatatct atccggcaag
     5701 aggagttcca caaccagttt gaaccatcat atataccggg aataacataa aaactggcat
     5761 aattcagatc cccccatttt cttaacccaa gaaagttgta acgcgaggga ttgtttctct
     5821 taatcggagc gcctaatatt tcttctccat tagtaaaagc ggaattaaat attggggctt
     5881 ctcccttgaa ggttatatct ggatcaaaag taagctgcca atcattcact atagcatctt
     5941 tgggtaatag aatattgatc tgtttaacag gtagctgtaa agctccagga gcaatgtaaa
     6001 agccataacc ctgttcatat aaagctgacg aagagaatcc ttcccttgga aaaggaaatt
     6061 gaatctgaac gctatacgaa ttagctaaaa ttagcgcagg aaagaatagt aaggcatata
     6121 ttaggtatcg cttcattttt tttacctcaa ccttatatag agcatttttt attccttgtc
     6181 attggaaatg ctgtaaccca ggcccttgcc agttactaaa acattaaaac gattgaaaat
     6241 ttgccgcaga cggtaaagca tagttttatc aggattaagt tcattaagca tcttcgctat
     6301 aaaggcatcc caattaactg tttcctgttt ctgaaaaaaa cctgtggagt gcatcacttg
     6361 gatgatgtag tgatggattt catccagttc ttctttactt gctgcattat agtatttatt
     6421 ttcaccttgc ttttcttcca gcggaagact gtaaatttcc caaagagcaa gagcaacagc
     6481 ctgagctaag tttatcgagg gaaaatcggg attagccata aaatgacagc gaagcggaca
     6541 aagctctgcc tcagaatctg ttaagccata agtttcgcgt ccaaaaacca gcccgatttt
     6601 agcattgggc acactgtgaa catatcttgc catctgcctt ggactaagat ctacaggttt
     6661 gttttttcca atgcgtcgag aaaaggcaat aactctatcc agatctttaa ctgcctcagc
     6721 cagagaagaa taaatgcaga cattttccaa tatatgttcg ctatgcattg ctaggtaaaa
     6781 atcgttcttt tctggggttt ttccgattat gcgaagctgc gaaaaacaaa aattattcat
     6841 aattcgagca atcgctccta cattcccgcc gtagattggt tctaccaaaa ttaccactat
     6901 ccgagagtga tcatagttgt aattcatcgt tacctggatg gatggttgtt tcctgaatta
     6961 gccacctttt ggttttgttg tgttttcttc cttttcttgt ttattatccc cgaaagcttt
     7021 gaacccatca tagatacctt gggattggaa tcatttcgcc aatccgcaat agttttttgc
     7081 gtaacattca taaattcgtt ggttttatcg cggcgactgc gttgcgaata gatattagcc
     7141 agctttttca tcgtttgcca aagagtttta atcctttttt catcaccttc aataagccag
     7201 tggcgaatgt tattgtaaca ttgtacagga cgttgcctgc ctacctgagt gaggatatgg
     7261 ctaacttttt tctgaacttc ttcgctctca tcatctaaaa gacgagaaac aaaagaaagc
     7321 acatactgcg gatgacgata agcaatattt tccattccgt gcatactgat cgcgcgtttc
     7381 ttttcgttat ccgattctgc ccaaagaggc atatattctt gcattacttc cggatctttt
     7441 ttccagagct caaaacagat ggtttccgat tcccaaggca cagtttcaaa ggttttatcc
     7501 aatgtattaa tgattcgttc cggatgctct tgatgcttat aatagaaata aaacaacgcc
     7561 gtagcacgaa ccccatagat aaaattctct aaaagccgtc cacagatatc atccaattct
     7621 ccctttccgg aatattgagc taagcgtttc cctagttctt cccgcacaaa ataatttgcc
     7681 gtattgccaa tgtctataat ttgtttttct gccaaagcac ttttaccttt atctaaattg
     7741 cgaaatatct tttctacttc ggcatcgagg atcttgtaat cgtctagatt taatcgtcca
     7801 acttctttta tcatttaacc tactttgtaa cggctatttg gataccgtca ctataattgt
     7861 tagtgtgttt tctgccactt gtaattttta atatttgaca ttaactaatt gatacccttc
     7921 ccttgttttc cctatatata ggattgggat ttaattggga ttgaagtgta tctacattaa
     7981 atacctttct atataaagat agctatctgt caagtaaaat ctgaaaatcg cggtttcttg
     8041 ccatcgtcat agttctatgc cctctgaagg ttcaaagttc tatgttcttg ttttagctca
     8101 caattttggt ttttttaaaa caagattcag gcttagttgt tagataaata gttagtagga
     8161 agctgatatt ccttagctga ttacaccaaa attgtaagac aaaacaagta tatcgccatt
     8221 catttttttt gttgatttcg gtggtctcgg tggctagaaa aaaatgcgga ggaacagcgt
     8281 cctccgtcta cacatctaaa tcctctaaat cctattcatc ctatgcctat tatcattttt
     8341 gaggggctta cgccaccgtc gctatccttg tgtcgcactt tgagttaaaa tcggctttgc
     8401 tcgtgatctt ccatacaatt ttattctttt tcctatcttt taaataggtc agtaataatc
     8461 tcatagaata ggccgat
//
LOCUS       DAOSTH010000068         8434 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_7981_length_8434_cov_13.643275, whole genome shotgun sequence.
ACCESSION   DAOSTH010000068 DAOSTH010000000
VERSION     DAOSTH010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8434
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7981_length_8434_cov_13.643275"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3251
                     /locus_tag="PLB65_06915"
     CDS             <1..3251
                     /locus_tag="PLB65_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOQ78295.1"
                     /translation="ALGYDINYTMEMQAVWYADNNLEMNSVTEPVNYTVNIEGPAITF
                     TGFANGWWLNPTFNTPLTFTVTVPQGRTIPNDGVWIDLYEVSAAGENQIQQMVLAPIS
                     VSGNVYSYSFNFGQLLSPMATAVKLYVEAIDNYNIVNQSQQTYGIDMAAPIVWALAPV
                     GAPIDNDGDGLFNEDVPNGVNEDLDWVDLDQDGFWDPEEPQIVDEDPIDYYPATLAQG
                     TDVVVAIAFEDYQGMQILMRGADSGRLNGKNSRTPIWYYTGASGIDVTNINVTLNGAA
                     ITGTITNGTFTHDAGVLPAGHYTVVAAVGDIVGNVGSLSYEFDVVGGAPTIAINPLNG
                     NWWLNTTGSNTFTFTVNSVAQLANGGVVANIYAEPSNTLIQGPITPTAGANNQYSVIL
                     YGGVVPADQTAVRLEVIATDVWGGTSTSNQTFLIDNNAPVITIHTPVAGTEVTYNEQV
                     SITATVTDNASTRGILKSSKDIQDRSGSGLQTVTMTVTPPTGTPVVFSNLTGAIAEIV
                     TANQYGNYTVAITAKDVVGNESVASTNFVVPAPAPEITFQPLANDSWWLNSTNNNKLS
                     FTINTAGINLAVGGVVANVYTIPANALFQGPVIPTAVNDVYSIDVMGGVIPVDQTGIR
                     LEVTATNILGGTSTSNQLYGIDNYAPLVTIINPTEDAQFSYNASVNIMATISEVVGTK
                     NNGQRSVSSDPKDKTGSGIAAVNLTVLAPDGNEAVNANYPENTQVIARQLVVNQYGTY
                     TINILATDNVNNQAMATRSFIVTPTVAPMISFTDIAWLNSIGLNNLDFTISAVVPVTV
                     TTNVITYPSGVTLMGPLTVSPVNGIYSVILNGSLIPAGETSVRLQVFATDQFGNATEA
                     NHYYGVDRSAPVITILNPVNGAEITLVDETTKVRIEAQISDLIPPLKNGSKTSAGSGI
                     AGSRMVIIDPLGLVISDVTEGAGITDITKELSNLMLGAYTVRVSAWDNAGNQAMTTIS
                     FTMINTPQPPAELEIVDAYAYPNPSTDGTAKFTVTLTEGAYVNIHIYDFAGREVRTLV
                     YSGKVQGKSKAEIVFDGRNNDGVKLARGAYFARVIANDGTKIVEKVVKIAIN"
     gene            3310..4266
                     /locus_tag="PLB65_06920"
     CDS             3310..4266
                     /locus_tag="PLB65_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78296.1"
                     /translation="MKQIKLVLIALILMISLSSVFAEDNSAAAYQLLGIDARSIGMGG
                     TGIAFLDNVSSAYLNPAVLADVKTIEFTSATRQNMVWDRNHNAVALGFVLPIGYVAAS
                     WQNATVDDIPGYDINGNPTGNFSNSDNTLALSYAAKIGKFNLGVTPKFYFSSQDDDSD
                     SGYGFDLGLLYHINRYFNAGMVVRDVIASYPGEEDKVPIEFIPGIAAYPIPGLIIAAD
                     LSGRDNFEEAKLRLGVEYWLGVKDDAEIGSSLSGIRIRESATWSDVLSKVQAGIRAGV
                     NDGAFTAGAGLRFKMLEINYAFQIAHDEYLNDTHSVSLLLRF"
     gene            4367..7093
                     /locus_tag="PLB65_06925"
     CDS             4367..7093
                     /locus_tag="PLB65_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOQ78297.1"
                     /translation="MKKIIIWLIILLSCQFALAIITDNIYYVNARQYYELGNYYSAKQ
                     NLDMMGAPDNQDPEYALLRGKVDLALGDFKQAYFWLTEYNKKSLGTDPLVQEDLLKML
                     NDVALYQEQTAISVSLGTLKGKINTGDSEYAPVLTPDGKYMYFSSLRRCEFKKENIFI
                     SLQQNNVWSEAVSISELCTDSNESFGSLSLDGNTAYLFGYYTKSNTSGDIYQTTQKNG
                     RWSKPTLIKEVSSNYYDLQPFVWRDKVMFLTSNRDGDHSNYDIYVSENINGTWSTPVN
                     IGNVINTPYDEQSPFLSEDGKNLYFASFGHNSFGGNDIFISTRIGNSWLEWSKPINMG
                     PIINSVKDDRYFVIAPDGKTAYLSSNRSGGIGQEDIYYIDLTLLGKIEEKIATLTVPE
                     TIPEEVPISFEELKISGVVVDNKNKPVQTDIIWVYHLEDKAYMRIISSESNGTFAFTL
                     PGNAQDIAFEINTPGYKKTTGALEIPSVKNEIFVNITLPIETEEIQGRNLAINGKVLD
                     EENNPVSCKIRFSYIYEDELNEVIVESDAQGTFKLYTPFVNKLKYKIEESGYAVREEM
                     ITLPEKANSYDMIIRLVKLGKEITLRGKIVDENDNPLVATVAWIYENEEEIVEYRVVT
                     DENGNYNISLPRMAKLEYGVSKPNYLQVSGEIDIPEDQQEITKNFTLSKLEKEAVFEL
                     KNVEFEFNKAVLTPKSIDILKPILATLKENETLEIELSGHTDNIGSRQVNKKISEARA
                     KAVADYFIQNGIDPKRIKTVGYGFDKPIASNDTPEGRQRNRRTEMKILGIEYQYDSFE
                     GSEKEFIEAEKKARIVKTVSKDSAYSTSQTGIPSSLETQFKTMILQNLASKKKLNLKV
                     DLFIDNGKIQSANVRDLMGNLDEKTVEEIADMMLGWKVQSQGRSIYTFSVEK"
     gene            complement(7748..8131)
                     /locus_tag="PLB65_06930"
     CDS             complement(7748..8131)
                     /locus_tag="PLB65_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78298.1"
                     /translation="MSLKRVLIATLSGILFGIVACLIASNSYTDPMAKKLLANIFASR
                     VLIGFVIGISACKIIWPLHGILMGIIVSLPYSFGAMAYQTPVLGKWHIFFLTLVLGAV
                     YGLLIELITSVIFKAKHTQEVIITQ"
BASE COUNT         2695 a         1443 c         1733 g         2563 t
ORIGIN      
        1 atgctctggg ttatgacatt aactatacaa tggaaatgca agcagtatgg tatgccgata
       61 ataatctgga gatgaatagt gtaacagaac ccgtaaacta cacagttaat attgaaggtc
      121 ctgcaattac ctttactggt tttgctaatg gctggtggtt gaatccaacc ttcaatactc
      181 ctttaacctt cacagtaact gttccacaag gcagaactat acccaatgat ggtgtctgga
      241 tcgatttata tgaagtatca gctgcaggtg aaaaccaaat ccagcaaatg gtccttgcac
      301 ccatttctgt ttctggaaat gtatatagtt attctttcaa ctttggtcag ttgctgtctc
      361 ctatggcaac tgcagttaag ctatatgttg aagcaataga taactacaat attgttaatc
      421 aatcacaaca gacctacgga attgatatgg ctgctccaat tgtttgggca cttgctccag
      481 ttggtgctcc tatagataat gatggtgatg gtttgtttaa tgaagatgtt cctaatggtg
      541 tgaatgaaga cctggattgg gttgatcttg atcaagatgg cttctgggat ccggaagaac
      601 cacaaatcgt ggatgaagac ccgattgatt attatcctgc aactcttgct cagggaaccg
      661 atgttgttgt agcaattgcc tttgaggact atcaagggat gcaaattcta atgcgaggag
      721 ctgattctgg aaggctaaac ggaaagaata gtagaacccc tatttggtat tatacaggtg
      781 ccagcggaat tgatgtaaca aacattaatg taactctaaa tggtgctgcc attactggta
      841 ctattaccaa tggaaccttc actcacgatg caggtgttct tcctgctggc cactatacag
      901 tagttgccgc agttggtgat attgtaggta atgttggatc tttatcctat gaatttgatg
      961 ttgtaggtgg agctcctact attgcaatta atcctcttaa tggtaactgg tggctaaata
     1021 ctacaggatc taatactttc acctttacag tgaactccgt agctcagctt gccaatggtg
     1081 gtgtggttgc taatatctat gccgaaccaa gcaacacatt aatccagggt ccgatcactc
     1141 ctacggcagg tgctaataat caatactctg taatccttta tggtggagta gttcctgcag
     1201 atcaaacagc agttcgttta gaagttattg ctaccgatgt ttggggtgga acttctactt
     1261 ccaatcagac cttcttgatt gataataatg caccggtgat tacaattcat actcctgtag
     1321 caggtaccga ggttacctat aatgaacagg ttagtattac agctacggtt actgataatg
     1381 caagtacgcg aggcatattg aaatcctcta aggatataca ggatagaagt ggttctggat
     1441 tacagactgt aactatgact gttactcctc ctactggaac accagttgta ttcagtaatc
     1501 tgactggagc tattgctgaa atcgtaactg ctaatcagta tggtaactat acagtagcta
     1561 tcacggccaa agatgtagtg ggtaatgaga gtgtagccag cacgaacttt gttgttcctg
     1621 ctcctgcacc tgaaattact ttccagccct tagcgaatga cagctggtgg ctaaattcca
     1681 ctaataacaa caagcttagc ttcactatca atacagccgg tatcaatttg gcggtaggcg
     1741 gagtggttgc taatgtttat actattcctg ccaacgctct attccaaggt cctgtaattc
     1801 ctaccgcagt aaatgatgta tacagcattg atgttatggg tggtgtaata cctgtagatc
     1861 agaccggaat tcgtttggaa gtgactgcta ctaatattct gggtggtact tccacttcta
     1921 atcaactcta tggtattgat aactatgctc ctttagttac cataatcaat cctacagagg
     1981 atgctcagtt tagttataat gcctctgtga atattatggc tactatttct gaagtagtag
     2041 gaaccaagaa taatggacag cgaagtgttt ccagtgatcc taaggataaa actggttcag
     2101 ggatagctgc tgtgaatctt actgtattgg ctcctgatgg aaatgaagct gtaaatgcta
     2161 attatcctga aaatactcag gttattgcaa gacagctggt tgttaatcaa tacggaacct
     2221 acacaattaa catcctggca accgacaatg tgaataatca agcaatggca actcgtagct
     2281 tcatagttac tcctacagtt gcgcctatga tttccttcac cgatattgcc tggttgaatt
     2341 ccataggcct taataacctt gatttcacaa ttagtgcagt agttcctgta actgttacca
     2401 ctaatgtaat tacctatcct tcgggagtaa ccctaatggg tccgcttact gtaagtccag
     2461 taaatggtat atacagtgta atattgaatg gtagtttaat tcctgcaggt gaaacttcag
     2521 ttcgcttaca agtatttgct actgatcaat ttggtaatgc cacagaagct aatcactatt
     2581 atggtgtgga tcgttctgct cctgtaatta caattctaaa ccctgtaaat ggtgctgaaa
     2641 ttactttagt ggatgagact actaaagtaa gaatcgaagc ccaaatcagt gatctgattc
     2701 ctcctttgaa gaatggtagc aaaactagtg ccggttctgg aattgcagga agtcggatgg
     2761 tgattattga tccattgggt ttagtaatta gtgatgttac agagggtgct ggcattactg
     2821 atattacgaa agaactatcg aatttaatgc tcggagcata cactgtgcgt gtatctgcct
     2881 gggataatgc aggcaatcaa gcaatgacaa caattagctt tacaatgatt aatactcctc
     2941 aacctcctgc agaattggag attgtagatg cttatgcata tcctaatccc agtactgatg
     3001 gaactgcgaa gtttaccgta accttaactg aaggtgctta tgtaaacatc cacatttatg
     3061 actttgcggg tcgtgaagtt cgcactttgg tttacagtgg taaagttcaa ggtaagagta
     3121 aagcagagat tgtatttgat ggtcgtaata atgatggtgt gaaattggca cgcggagcat
     3181 attttgcacg cgtgattgcc aacgatggca cgaagatagt agaaaaggta gttaagatag
     3241 ctatcaacta aattggtgaa gaggggagga ggatatcctc ctcccttcca accgaaagga
     3301 aggaaaaaaa tgaaacaaat taaattagta ctgatagccc tgatactgat gatttcactg
     3361 tcttctgtct ttgctgaaga taattctgca gcagcatatc aattgttagg tattgatgct
     3421 cgttcaattg gtatgggtgg aactggaatc gcgttcttgg ataatgtctc aagcgcatat
     3481 ctgaatccgg cagttttggc agatgttaaa acaatagaat ttacttcagc cacaaggcag
     3541 aatatggttt gggatagaaa tcacaatgca gttgctttgg ggtttgtttt acccataggt
     3601 tatgtagcag catcttggca aaatgctaca gttgatgata ttccaggata tgatattaat
     3661 ggcaatccta caggtaattt tagcaatagt gataatactt tagcgttaag ctatgctgcc
     3721 aaaattggga aattcaattt aggtgttact ccaaagttct atttttccag ccaggatgat
     3781 gattccgatt ctggttatgg ttttgatctt ggattactct atcatatcaa tcgctatttt
     3841 aatgcaggaa tggtagttcg agatgtaata gccagttatc caggagaaga ggataaagtc
     3901 ccgatcgaat ttattcctgg tattgcagct tatccaattc ctggtttgat tattgctgca
     3961 gatctttccg gacgcgataa ttttgaagaa gcaaaattgc gtctgggagt tgaatattgg
     4021 ttaggcgtga aagacgatgc cgaaattggt tccagtttat ccggaattcg tattagagag
     4081 agtgcaacct ggtctgatgt cttatccaaa gttcaggctg gaattcgtgc cggagttaat
     4141 gatggtgctt ttacagcagg tgcaggacta cggttcaaga tgttagaaat taattatgca
     4201 ttccagatag cacacgatga atacttgaat gatacacatt cagtttctct gttactacgt
     4261 ttttaaaaca taggtaactg ggatatctgg tatgcaataa aaagttgtga ttggtttccc
     4321 ctgagtgttt ttccttaggg gaaaccatta caatagagga gatataatga agaaaataat
     4381 aatctggtta ataatcctcc tatcttgcca gtttgctctc gccataataa ctgataacat
     4441 ctattatgta aatgccaggc aatattatga attaggtaat tattactctg ccaaacaaaa
     4501 tttggatatg atgggagccc cagataatca ggatcccgaa tatgctcttt tgcgaggtaa
     4561 agtagatctg gctttgggag atttcaaaca agcctatttt tggttaaccg aatacaataa
     4621 aaaatctttg ggaacagacc ctttagttca ggaagatttg cttaagatgc tgaatgatgt
     4681 tgctttgtat caagagcaga cagctatatc tgtatctttg ggtacactga aaggtaaaat
     4741 aaacacagga gattcagaat atgcacctgt acttactcca gacgggaaat atatgtattt
     4801 cagttcactg cgtagatgtg agtttaaaaa agagaatatt ttcatttctc ttcaacagaa
     4861 taatgtctgg agtgaagcag tttcgataag cgaattatgt accgacagca atgaatcctt
     4921 tggttccctt tctcttgatg gaaatactgc ttatttattt ggatattata caaaatctaa
     4981 tactagtgga gatatctatc aaaccacaca aaagaatgga cgctggagta aaccgaccct
     5041 gattaaagag gtctccagta attattatga tttgcaacct tttgtttggc gagataaagt
     5101 tatgttttta accagtaacc gtgatggcga tcattcaaac tatgatattt atgtgtcaga
     5161 aaatattaac ggaacctggt caacacctgt taatattgga aatgtaatta atactcccta
     5221 tgatgagcaa tcaccatttt taagtgaaga tggaaagaat ctctattttg cttcatttgg
     5281 gcataacagt tttggtggca acgatatttt tatttctacc agaattggta attcttggct
     5341 ggaatggagt aaacctataa atatgggacc tattattaat tctgtgaaag atgatcgtta
     5401 ttttgttatt gctcctgatg gcaaaacagc atatttatca tctaatcgtt caggcggtat
     5461 tgggcaagaa gatatatatt atatagattt aactctatta ggcaagatag aagaaaaaat
     5521 tgctacttta accgtgcctg aaactatccc tgaagaagtt cctataagtt ttgaggaatt
     5581 aaaaatcagt ggtgttgtag tagataataa aaataaacct gtacagacag atataatatg
     5641 ggtttatcat cttgaagata aagcatatat gcgcattatt tcttcagaga gtaacgggac
     5701 ttttgccttt actcttccag gaaacgcaca agatatagct tttgagatta atactcctgg
     5761 ttataaaaaa actacaggag cgttagaaat tccctcggtt aaaaatgaaa tattcgtaaa
     5821 tatcactttg cctattgaaa cagaagagat tcagggaaga aatcttgcca tcaatggaaa
     5881 agtattagat gaagagaata atcccgtctc ttgtaaaatt cgatttagct atatatatga
     5941 agatgagctg aatgaagtta ttgtggaaag tgatgcgcag ggtactttta aactttatac
     6001 tccctttgtt aataagttaa agtataagat cgaagaatca ggttatgctg ttcgtgaaga
     6061 aatgatcact ttaccagaaa aggcaaatag ctatgatatg atcattcgtt tggtaaaact
     6121 gggcaaagaa attacgctaa gaggcaagat tgttgatgag aatgataatc ctttagttgc
     6181 tactgttgct tggatttatg aaaatgaaga ggaaattgta gaatatagag ttgttacaga
     6241 cgaaaatggc aactacaata tttcccttcc cagaatggcc aaacttgagt atggagtttc
     6301 taagccaaat tatctacagg tatcgggtga aatagatatt cctgaagatc agcaggaaat
     6361 aacaaagaac tttacattgt cgaaactgga gaaagaagct gtatttgaac tgaaaaatgt
     6421 agaattcgaa ttcaataaag cagtattaac tcccaaatct atcgatattt taaaacctat
     6481 tttggctact ttgaaagaga atgaaaccct ggaaatcgaa ctatctggcc acacagataa
     6541 cattggaagc aggcaagtga ataaaaagat ttcggaagca cgggctaaag cagtcgctga
     6601 ttattttatt caaaatggaa ttgatcctaa acgaattaaa actgtcgggt atggatttga
     6661 taaaccaatc gcttctaatg atactccaga aggcagacaa agaaaccgca gaaccgaaat
     6721 gaaaattttg ggcatcgaat atcaatatga ttcttttgaa ggttcggaaa aagaatttat
     6781 agaagcagag aaaaaggctc gcatcgtaaa aactgtctct aaagattctg cttactctac
     6841 tagccaaaca ggtattcctt cttccttaga aactcagttc aaaactatga ttctgcaaaa
     6901 cttggcttcc aagaaaaagc ttaatttaaa agttgatctt ttcatcgata atggtaaaat
     6961 ccaatctgct aatgtgcgag acctaatggg caatctggat gaaaagactg tggaagaaat
     7021 tgcagatatg atgctgggtt ggaaagtgca atctcaagga cgcagtattt atactttctc
     7081 tgtagaaaaa tagtatatcc ttatacttat cgttaataag ggttccgaaa ttcagtttgg
     7141 gacccttttt tgtgtgtaat ctgccccaca tataaatagg gaaatatgga tccgaaattt
     7201 agtcagtagg cttattacgc ttggctatga atcctttctt gaaatgaaaa gataaaaaaa
     7261 gctggtgttg agttccagct tttgccatca ttatagcgat aatataaaag caatgaaaac
     7321 aatataatat gaagataaaa ccataaaacc gtatcctgga tatttcaatt tagttttatg
     7381 cttgctttat taggtgcggt gtagctgcaa aggtgcttct cggaattttg ccgagtagta
     7441 ccaccgtatc cctacgacag atatctaagg aagaaaaaaa ttatatttca acaatgaagg
     7501 tgctatatct gagattcaga ggacccttaa aaaaatagtt gcattaggaa cgaaaggtga
     7561 tagttattaa tattggtatt ggtggaaaag aaaaaacatc accattatgc gaagaaattt
     7621 gatatcttct tccaaagaaa aatgaagaga attaactaga aaaactgcga aatgctgttt
     7681 cttatatctt aaaagtactt acctcattgc tacaaaatga aattgttata atatcaaggc
     7741 aaaactctca ctgagtaata attacttcct gtgtatgttt tgctttaaaa atgacactgg
     7801 ttatcagttc gatcaataaa ccataaacag cacccaaaac aagagtcagg aagaaaatat
     7861 gccattttcc caaaactggt gtctgataag ccatcgctcc aaaggaataa ggcaaactga
     7921 caattatccc cataaggatt ccatgcaatg gccagattat cttgcaagca ctaatcccga
     7981 taacaaaacc aatcagaacc cgactggcga agatattggc taataatttc ttagccatgg
     8041 gatcggtata gctgttactt gctatcaagc aggcaacaat cccgaaaaga attccggaaa
     8101 gggtagcgat taaaactcgt tttaaagaca taatcacctc actaagtttg attatttcta
     8161 taaaagcgca gggcaataat aatgtcaata acttttttta aaagatatcg ataaattgcg
     8221 ggattctata aaataagatc gttgcacata aatggagcaa aaatctttta cttcctaagc
     8281 ggggaagcag tatgctcaga gacgaacccg gttgtcggat ttagaacaca aaaaccgttt
     8341 ctaccctttc attggaagtg taggactacg actattgtat ctatatgatg gttgattact
     8401 tcagggagat catctgaaaa gggtaacctt ttta
//
LOCUS       DAOSTH010000069         8333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_8102_length_8333_cov_12.306233, whole genome shotgun sequence.
ACCESSION   DAOSTH010000069 DAOSTH010000000
VERSION     DAOSTH010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8102_length_8333_cov_12.306233"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            105..1016
                     /locus_tag="PLB65_06935"
     CDS             105..1016
                     /locus_tag="PLB65_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HOQ78299.1"
                     /translation="MNIFLGIDIGGSSFKYGLGNCEVGLQYYQILNLPEKNLNTFLSV
                     ASKMFNDVDNQFGLNKLKGIGIGIPGAIDKITGCAIGTNPNLPFWVNHSPLELIPDNC
                     KLPLAFDNDANLMALSEANSYCKQYAIGVTIGSGIGCGIISDGQILHGANGYAGELGH
                     ICVVDNGAKCNCGKNGCLEAYSSVEGLRNLLAIHNPLYSKLDLPTLVAKKETDPLVNE
                     YCKQGKQLLSIALAGLATCLDPEIIILGGGAMDFDLYYISEIEKDILELLPKAHRAKI
                     SITKAKNGNKAGVLGAIKLIEEKFYGN"
     gene            1064..1420
                     /locus_tag="PLB65_06940"
     CDS             1064..1420
                     /locus_tag="PLB65_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78300.1"
                     /translation="MRKIMLTMVLMAVLMMLATAGYALKSSPVECFDISPNPMYKDCE
                     IYVAVSAPLSINIQIQSLDGKVIRDIYSGLVGKDITLYWERTDNWGNYVPDGEYFVVL
                     GFSTRYTSLKKTLILK"
     gene            1451..2212
                     /locus_tag="PLB65_06945"
     CDS             1451..2212
                     /locus_tag="PLB65_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOQ78301.1"
                     /translation="MQYKRKKSLTDFFLFLEEATTELFHIRGNRHVSLMVVIRQILFT
                     GVEALPLIGFIALAIGCLTIMQGYAFLSNFGQGIWVHIILVRVIVGELSGLITALVVI
                     ARSGTAISTELGNMVVNREMQLLKSFDIAPMGYLVVSRILGVVISMLVLTLYFNIIAV
                     LGGWLFAQFFNHLSFSAFMNDFFSILTFPHIIMTVLKSIVFGFVIAITASYQGMSVSK
                     ASTEVPQRTIKAVVNSIVAVIIVDIIISWLFWIGV"
     gene            2212..2919
                     /locus_tag="PLB65_06950"
     CDS             2212..2919
                     /locus_tag="PLB65_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ78302.1"
                     /translation="MHIAFENIVIKGLVNNLNLEFDTKGCTVLYDPCEIVSSTILSAL
                     IGLYELDGGNIYLDGIPKQEYFLNHSLISTFSMIFNEGIMLANLTVKENLLLPYHRRF
                     QIVETSIFEKELKFFMQVFNQDTDLELRPAFLTSSQRKFFCFIRSLLLKPKILLIDDP
                     YYLFNRNDRKQIYDFLKRFSSSSGIDIDFATKHNNIQDSYNYEKNGIPEMLISTSDED
                     FTDNFATQVIDLSQFKS"
     gene            complement(2916..5351)
                     /locus_tag="PLB65_06955"
     CDS             complement(2916..5351)
                     /locus_tag="PLB65_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HOQ78303.1"
                     /translation="MRKTTFCLLLLMLSISLSFGQYIVNFEGADETKTAYASGDVTLS
                     GLSWNLTEVLIGNLATDWKNGTKSARLRGYGTSAMTMLENKTSGLGTLSFYYRRYATD
                     AQVDWKVEYSIDNGSSWMQIGSVFTAPASDTPSLFSEDVNVSGDVRIRIKRATETGTS
                     NNRLNIDDITLTDFTGIEVEPPTLQASNIIAYPANTEITLEWTPGNGARRLVKINTIN
                     SFTSPVDGSNLAANDVYSGIGEQVIFNGSTQIVEGLPLNGCNVSGLTPLTTYWFRIYE
                     YNGSGSFTRYNTSESTNNPISAITTNTPGSDYYTGITGYGITLKTNLHNLLRTTHTTR
                     YSYDALWIKLPYTDEDPTNTNNIIEIYTGWSVPKAYYGSGTSQWNREHTWSKSHGNFS
                     ETPPAGTDLHHLRPCDSTVNSAKSNKDFDYATTSYYDSSPYNGYPQDTGCKTSTYAWE
                     PRDADKGDVARMIMYMAVRYEGTDTSYDLEIVDYTNTSGPYYGKLSTLLEWHIIDPPD
                     SIEMERNNRIEELQGNRNPFIDEPLYAVQIWAPMPQNPTNITQTSFTANWSAPISATK
                     YYLQLATDASFNNIVPGYDNLDAGLILSKTISGLSNTNTYYYRLRSFFVSGYSMYSPY
                     MEVTLSASAQLNSGTALTEYNLNAAILQLNLVDTTWRDNILMLANFTINNAPDGLSLL
                     SVQYLSSNSALLVMHFNNADFDDNILDFCVTIDAAEINYPSSITTNSLAIIAYVESPL
                     TIQIQGQQLILNIQTVAEADAYVVFSSIEPYGIYSEISSSGNFDAQIPTRWTCILPTE
                     NIRFYKAAAVKYP"
     gene            5539..6099
                     /locus_tag="PLB65_06960"
     CDS             5539..6099
                     /locus_tag="PLB65_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78304.1"
                     /translation="MERIAFILLITLLLCSCDLFTVRDSEHPGAETPWIDFATECDSV
                     LLNLEYSYEYVQNAVHYSGIFTPDYVFHFAPQDISDLSIEQTWTRVQEQDMIQVLHNR
                     YKEMQVTLEPVETQDQIESTQAKIYRQYLITGIFRSEGKERLVLASGNLELHLKKEYG
                     YWYLNQWYDYRSSNSPTWGRLKYENH"
     gene            6086..6661
                     /locus_tag="PLB65_06965"
     CDS             6086..6661
                     /locus_tag="PLB65_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78305.1"
                     /translation="MRTISIILIFLTFLIGSCSNPFCPELNDVSSAEIQNRTPEELLQ
                     NLERAYTEKNINLYKSLLHPDFRFELLASEVSLIGIDMNGDGMRDSWWGYDQEIEYTD
                     RMFNRGSSDGTYYPPDEIKLRLQIPPSDRWQTDPEVGHEDWIIIPCNFDLILSYKESN
                     SLFNANGIARFYLKPVKNGWQIAIWRDESNI"
     gene            6658..7074
                     /gene="ruvX"
                     /locus_tag="PLB65_06970"
     CDS             6658..7074
                     /gene="ruvX"
                     /locus_tag="PLB65_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOQ78306.1"
                     /translation="MNPEGRILALDYGEKRIGLALSDPNRIFAKPLCILSNKGFEQLI
                     CELQEIIVNNQVTMLIVGIPYAIDGSFTAKTEECQSVLDRLQEVLTIPVEGFDERYST
                     WEANEELKKMGYNWQQARKIKDAMAAAIFLREYLSK"
     gene            7071..8054
                     /gene="mltG"
                     /locus_tag="PLB65_06975"
     CDS             7071..8054
                     /gene="mltG"
                     /locus_tag="PLB65_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOQ78307.1"
                     /translation="MNKKKVVIISLAIVVFLVMLYIAYLIFVPKHSEELIVRIKPGDN
                     ARVIANKLSESGIIRSKTMFIWLTKLRNADRNLKPGSYIFGGNTYLWQTVTRLYKGQS
                     ENITITFPEGLSLYKTLKRIDASGLATFEELSAKAKDPTVVNKLTGFDVPSLEGFLYP
                     ETYRFPIDISADSILAIQTAEFFKKLNQEGIDPYSTPNFYNKLILASIVEKEAGTESE
                     KEIIAGLFMNRMAIGMALQSCSTVDYLLEPKGIKRDVLTYTDTQINSPYNTYIYQGLP
                     PTPICNPSVNTIKAVLNAKPNSYLYFFSDRKGKNVFSRTYEEHLAKQRQML"
BASE COUNT         2582 a         1409 c         1590 g         2752 t
ORIGIN      
        1 accggattgt ggttccgggc gttgtgggtt caaatcccat cacccacccc attttttttc
       61 ttagcgatct tactgtaaac aaaatgaata gatgaagcaa aattatgaat atattcttag
      121 gcatagatat tggcggttcc agttttaaat atggtttggg caactgtgaa gtaggtttac
      181 aatactatca gatcctcaat ttaccagaga agaatttgaa cacattttta tcagttgctt
      241 ctaaaatgtt caatgatgta gacaaccaat ttggtcttaa taaattaaaa ggtattggaa
      301 ttggcattcc tggcgctatt gataaaatta caggttgcgc tattggtaca aatcctaatt
      361 tacctttttg ggttaatcac agccctcttg aactgattcc cgataactgt aaacttccct
      421 tagcatttga taacgatgcc aatttgatgg ctttgtccga agccaatagt tactgtaaac
      481 aatatgctat tggagttacc attggtagtg gtattggttg tggaataatt tctgatggcc
      541 aaattcttca cggtgcaaat ggctatgctg gagaacttgg ccatatttgc gttgtagata
      601 atggagcaaa atgtaattgt ggcaagaatg gctgtttgga agcatattca tcagttgagg
      661 gactacgcaa tctattagct atccacaacc ccctgtactc taaattagat ttaccaactc
      721 tagtggctaa aaaagaaact gatcctttag taaacgaata ttgtaaacaa ggtaaacagt
      781 tattatctat tgccttagca gggcttgcca cttgtcttga tcccgaaata ataattttgg
      841 gtggcggagc aatggatttt gatttatatt atattagtga gatagaaaag gatattttgg
      901 agcttttgcc taaagcacat agagctaaga tatcgattac caaagcaaaa aacggtaata
      961 aagcaggagt tttaggtgct ataaaactta tcgaagagaa gttttatggt aattaggaat
     1021 gaatgttgca taatatattt acatcaataa ttaggagtaa aaaatgagga agattatgct
     1081 tacgatggtg ctgatggcag tgttaatgat gttagccaca gctgggtatg ctttaaagag
     1141 ttctcctgtt gagtgttttg atatttctcc caatcctatg tataaggatt gtgagatata
     1201 tgttgctgtg tctgctcctc tgagtattaa tatccaaatt cagagtttag atggtaaagt
     1261 tattcgtgat atttacagtg gtttggtagg caaagatata actttgtatt gggagcgaac
     1321 tgataattgg ggcaattatg taccggatgg tgagtatttt gtagttttgg gatttagcac
     1381 ccgttatacc tctttaaaga agacacttat tctcaaataa caacgctttt tttaagcgat
     1441 tatgtaactt atgcaataca aaagaaaaaa gtccttaacg gacttttttt tgtttttaga
     1501 agaggcgaca accgaattat ttcatattcg aggcaatcgc catgtaagtt taatggttgt
     1561 catcaggcag attctgttta caggtgttga ggcattaccc ttgataggtt ttatcgcttt
     1621 agctattggg tgtttaacga ttatgcaggg ttatgcattt ttaagcaatt tcggacaggg
     1681 aatttgggtt catataattt tggtaagggt tatagtgggg gaattaagtg gtcttattac
     1741 agcgttagta gttattgcca gaagtggaac tgcgatttct acagaattgg gaaatatggt
     1801 agttaaccgg gaaatgcaat tattgaaatc ttttgatata gcaccaatgg gatatttggt
     1861 tgtttctcgt attttgggag ttgtaatttc tatgctggtg ttaacattat atttcaatat
     1921 cattgcagtt ttgggtggtt ggctttttgc gcagttcttt aatcatttat cctttagtgc
     1981 gtttatgaat gatttctttt ctattcttac ttttcctcat attataatga ccgttttaaa
     2041 atctattgtt tttggttttg taatagcaat tactgcttcc tatcagggaa tgagtgtttc
     2101 caaagcatca actgaagttc ctcaacgcac gataaaagct gttgttaatt ccattgtagc
     2161 tgtaattatt gtagatatca taatctcttg gctgttttgg attggtgtgt aatgcatatc
     2221 gcttttgaga atatagtaat caaaggttta gtgaacaacc tgaatttgga gtttgacact
     2281 aagggatgca cagtattata tgatccttgt gaaattgtat cttccactat cttatctgct
     2341 cttattggac tttatgaatt agatggcggt aatatatatc tggatgggat acctaagcaa
     2401 gagtattttt taaaccactc tctcatttct actttttcta tgatttttaa cgaaggaata
     2461 atgctggcca atctaactgt gaaagaaaat ttgcttcttc catatcatag gcggtttcaa
     2521 atagtggaaa caagcatctt tgaaaaagag ttgaagttct ttatgcaggt ctttaatcag
     2581 gatactgatc tggaattgcg tcctgctttt ttaacatctt cccagcggaa atttttctgc
     2641 ttcatccgtt cgttgctctt aaaacctaag attttattaa ttgacgatcc ctattatctt
     2701 ttcaatagaa acgataggaa acagatatat gattttttaa agcggtttag ttcttcctct
     2761 gggattgata tcgatttcgc tactaagcat aataacatac aagatagcta caattatgaa
     2821 aaaaatggaa ttccagaaat gctgattagt accagtgatg aagattttac cgataacttt
     2881 gccactcagg ttatagacct ttctcaattt aaatcttaag gatatttaac cgcagctgcc
     2941 ttataaaatc ttatattctc agttggtaaa atacaagtcc aacgcgttgg aatttgagca
     3001 tcaaaattgc cgcttgagct tatctcagaa taaataccgt atggttctat gctggaaaaa
     3061 acaacatagg catcggcttc tgcaaccgtt tgaatattta aaattagctg ctggccttgt
     3121 atttggatag ttagcggact ttctacatag gcaatgatcg ctaacgaatt agtcgttata
     3181 ctagatggat aatttatttc ggcagcatct atagtaacac aaaaatccag aatattatcg
     3241 tcaaaatcag cattattaaa atgcataacc agcaaagcac tattagaact taaatattga
     3301 acacttagca aagaaaggcc atcaggagca ttatttatag taaagttggc tagcataagg
     3361 atgttatctc tccaggtagt atctaccaaa ttcaattgta agatagcagc attcaaatta
     3421 tattccgtta aagctgttcc agaatttagt tgtgcagaag cagaaagagt tacttccata
     3481 tagggagaat acatactgta gccactaaca aaaaatgaac gcaagcgata ataataagta
     3541 ttggtattgg ataagccact aatagttttg gaaagaatta aaccagcatc cagattatca
     3601 tatccgggaa caatgttatt gaaagaagca tccgtagcca attgcaaata atattttgtt
     3661 gctgatatgg gagcagacca atttgccgta aaactggttt gagtgatatt agtaggattt
     3721 tgtggcattg gtgcccagat ttgaactgca tagagtggtt catcaataaa aggattacgg
     3781 tttccttgca attcttcaat ccggttattt ctttccattt caatggaatc gggaggatca
     3841 attatatgcc attctaataa tgttgagagc tttccataat atggtccact ggtattggta
     3901 taatccacaa tttccaaatc gtaggaagta tccgttcctt cataacgaac tgccatatac
     3961 ataatcattc tggctacatc acctttatcc gcatctcggg gctcccaagc ataggtagat
     4021 gtcttacatc ctgtatcttg tggataaccg ttatagggag aagaatcata ataagaagta
     4081 gtagcataat caaaatcttt attacttttg gcagaattta ccgtagaatc gcaagggcgc
     4141 aaatgatgca aatcagttcc agcagggggt gtttcactaa aattaccatg acttttactc
     4201 caagtatgtt cccgattcca ttgtgaagtt ccgctgccat aatatgcttt gggaacactc
     4261 caacctgtat atatttcgat aatgttatta gtattagtcg gatcttcatc tgtgtagggt
     4321 aattttatcc acaaagcatc atacgaatag cgagttgtat gagtagtccg aaggagatta
     4381 tgcagattcg ttttcagtgt aattccataa cctgtgattc cagtgtaata atcactaccg
     4441 ggagtatttg tagtgatagc agaaatagga ttatttgtgg attctgatgt attatagcga
     4501 gtaaaactac cgctgccgtt atattcataa attctaaacc aataagttgt tagaggtgtt
     4561 aagccagata cattacaacc atttaaagga agtccttcta ctatttgtgt tgaaccatta
     4621 aagataactt gttctccaat accactgtaa acatcgttgg cagcaagatt gctgccatca
     4681 acgggagaag taaatgaatt aatggtattg attttaacta agcgtcgtgc cccattacca
     4741 ggagtccatt ccaaagttat ttcagtattg gcaggataag caattatatt tgaagcttgc
     4801 aaagtggggg gttcaacttc tatgcctgta aaatccgtta aagttatgtc atctatattt
     4861 aagcgattat tagaagttcc tgtttcggta gcgcgtttaa ttcttattct cacatcccca
     4921 ctaacattta catcttcaga aaataaactg ggtgtatcac ttgcaggagc tgtaaaaaca
     4981 gaaccaattt gcatccaact actgccattg tcaatactgt attctacttt ccagtccact
     5041 tgcgcatcag tagcatatct tctataataa aaagaaagag tgcccaaacc actagttttg
     5101 ttttctaaca ttgtcattgc agaagtccca taacctcgca acctagctga tttcgtacca
     5161 tttttccaat cagtagctaa attaccaatt aacacttctg tcagattcca agaaagaccg
     5221 cttaaagtta catcacctga agcataagca gttttggttt cgtccgctcc ttcaaaatta
     5281 actatgtatt gaccaaagct tagtgaaata cttagcatta aaagcagcaa gcagaatgtg
     5341 gttttccgca ttgagtaact ccctttgttt attatatatt atccttttcc cttgcttcct
     5401 caaattgtca agccaaatcc taaatttgtc tatggggtgc tctgtttttt tagcggtaca
     5461 caggactctt aattatttta gcatccaaaa tccacagatt ttgattgaca taatatttaa
     5521 aatccttttt atgaacttat ggaaagaatc gcttttattt tgctgataac tcttttgctc
     5581 tgtagctgtg atttatttac agtacgagat tcagagcatc caggagcaga aacaccgtgg
     5641 atagattttg ccacggaatg tgattctgtg ttattaaatc tggaatatag ctatgagtat
     5701 gtgcaaaatg cagttcatta ttcaggtatt tttacacctg attatgtttt tcattttgca
     5761 ccgcaagata taagtgattt gtctatagag caaacttgga caagggttca ggagcaggat
     5821 atgattcaag ttttgcataa tcgctataaa gagatgcagg ttactttaga gcctgtggaa
     5881 actcaggacc aaatagaatc cactcaagcc aaaatatatc gtcaatacct tataaccggg
     5941 atttttcgca gtgaaggaaa agagcgttta gttttggcta gcggcaatct tgaattacat
     6001 ttaaagaagg aatatggcta ctggtattta aatcagtggt atgattatcg cagtagcaat
     6061 tctccaacct ggggaaggtt aaaatatgag aaccattagc ataattctaa tattcttaac
     6121 ttttctgatt gggagttgta gcaatccctt ctgtcctgag cttaatgatg tttccagtgc
     6181 tgaaattcaa aataggacac cggaagaact tttgcaaaat ctggagcgcg catacacaga
     6241 aaaaaatatc aacctttata aatcgctctt gcatcctgat ttccgttttg aacttttggc
     6301 aagcgaggtc agtttaattg gtattgatat gaatggagac ggaatgcgag attcttggtg
     6361 gggttatgac caggaaattg aatatacgga tagaatgttc aatcgcggtt ccagtgatgg
     6421 aacttattat cctcccgatg aaataaaact gcgtttacag attcctcctt ctgatcgatg
     6481 gcaaaccgat cctgaggttg gtcatgagga ttggataata attccctgca attttgattt
     6541 aatcctatcc tataaagaaa gtaattcctt gtttaacgcc aatggcatag ccagatttta
     6601 tttaaaaccg gtaaaaaatg gctggcaaat tgccatttgg agggatgaat ccaatatatg
     6661 aatcccgaag ggcgaattct tgccttggat tatggtgaaa aaagaattgg gcttgcttta
     6721 agtgacccta atcgcatatt tgccaaaccc ttgtgcattc ttagtaataa aggttttgaa
     6781 cagcttattt gcgaattaca agaaattata gttaataatc aagtaacaat gctcattgtt
     6841 ggcattccat atgccattga tggttccttt actgctaaaa ctgaagaatg ccaatctgtt
     6901 ttagatcgtt tacaagaagt tcttactatt ccagttgaag gatttgatga acgctattct
     6961 acttgggaag caaatgaaga attaaaaaaa atgggatata attggcagca agcaagaaaa
     7021 attaaagatg ctatggcagc tgccatcttt ttaagagagt atttatctaa atgaataaga
     7081 agaaagttgt tatcatcagt ttggcaattg ttgttttttt ggttatgtta tatattgcct
     7141 atctgatttt tgtacccaag catagcgaag aattaattgt gcgtataaag cctggagata
     7201 atgccagagt aattgccaat aaactttccg aatcaggaat tattcgcagt aaaacaatgt
     7261 ttatttggct taccaaacta agaaatgcag atagaaacct aaaaccggga agttacattt
     7321 ttggaggtaa tacctatctt tggcaaaccg ttacccgctt atataaaggt caaagcgaga
     7381 acattacaat cacctttcca gaagggcttt ctctatataa aaccctgaaa agaattgatg
     7441 ccagcggctt agctactttt gaagaattat cagcaaaagc aaaagatcct acagtagtta
     7501 ataaacttac cggatttgat gtcccatctc tggaaggatt tttatatccg gaaacatatc
     7561 gttttccaat tgacatttca gcagattcca ttttggcaat tcagactgca gaatttttca
     7621 aaaagctaaa ccaagaagga atcgatcctt attctacgcc caatttctat aataaattaa
     7681 tccttgcttc cattgtggaa aaagaagcag gaacagaaag tgaaaaagaa attattgctg
     7741 ggctttttat gaatcgaatg gcaattggta tggctcttca atcttgttcc acagtagatt
     7801 atcttcttga gccaaaaggc attaaacgcg atgttttaac ctatactgat acccagataa
     7861 attctcctta taacacctat atttatcaag gtttacctcc tacgccgatc tgtaatcctt
     7921 ctgttaatac aataaaagcg gttttgaatg ccaaacctaa ttcgtacctc tattttttct
     7981 ctgatcgcaa agggaaaaat gtgttttccc gaacctacga agaacattta gccaaacaaa
     8041 ggcaaatgct ataaaatctg tgttatttgt gagaagtact tatatctctt tttcggtgat
     8101 ctcggtgtcc tcggtggcta agaaaatcct ctaaccctta aatccttatt tctaaaacta
     8161 aatgggtgta atttatgtag tctgcgtgaa ttattttcgg tgatttcggt gtcctcggtg
     8221 gctaaaaaac cccgtttcta aaatcaaatc tgtgttatcg gtgagaagct accatcattt
     8281 attttcaagg cattcccaca aaaaagtgat gtggacaagt tataacacac tgt
//
LOCUS       DAOSTH010000070         8019 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_8493_length_8019_cov_11.641637, whole genome shotgun sequence.
ACCESSION   DAOSTH010000070 DAOSTH010000000
VERSION     DAOSTH010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8493_length_8019_cov_11.641637"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="PLB65_06980"
     CDS             complement(<1..988)
                     /locus_tag="PLB65_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HOQ78308.1"
                     /translation="MHKIKSMCFIIGCLALVIMLSGCLQKVEKTPVNYYVLEYQPGSE
                     KPELRRANNTGKNLEVLDTVLPRTYDRNQIVVKENFYKVKFLQTDVWATRLRDAIPNL
                     VVQRVKAYNIFGTVSRGEILDKEPHYFLETTVYNIEKIEGAEPRAYLKMEFVLRDSTS
                     ERIVLSHKNERSVELIDPSMIYLVQAFNDMIMEETDLFAAKCNLYFSGHKIDEKSLAT
                     SASPMARYVYEEMFASETVNDYGELMVITKTQTEEELRYTIEELDSLNSVISTDELVM
                     GVPALLRPGRYRVIIGEMEDLIIPVQILPRQRTVVKPNWGELKIVIMDESKSR"
     gene            complement(997..2229)
                     /locus_tag="PLB65_06985"
     CDS             complement(997..2229)
                     /locus_tag="PLB65_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOQ78309.1"
                     /translation="MVSKAAKIRLGVFLTIGAILIIIFAAIVAGSRLVEKKDIYYIEF
                     EDYSVSGLQVGSSVNYRGIKIGRVEDIKINPKDVTKIIVSISVNRGTPIKEDTEAVLL
                     LSGITGLKSVEIRGGTNEAKLLKPKSYIKPGTTMLEDISERAKSIVDKIDMIAANLNL
                     ITGEENRQNISKILEQTSLILKDTHENLATTMQSISRIANNTADLTEELSRDLSAVTD
                     NLTKNLDAITNSATSNIQSVSETSQTSLISVTSNVNKQLEELTSQLEYSLARLTEDGS
                     ALIREANLKVTTVGEHSDQMILETTREILTISNNINKTLDQVNAILYTPGVDSLMTNL
                     GTLCGELSRTNINEMVSELSTTIHKTGNLVANLNRVVTRGQGDLLEILSTLTETSENL
                     NEFSRQIADTPAILLRGN"
     gene            complement(2232..3626)
                     /locus_tag="PLB65_06990"
     CDS             complement(2232..3626)
                     /locus_tag="PLB65_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HOQ78310.1"
                     /translation="MSTPFIRLMKQEGIREDVIRTFEAYYKMLVKGEKGLIKEEEISP
                     PAEENLIDYQQIRQNSRSSMLKNTAVIKLNGGLGTSMGLSKAKSLLPVKNNLNFLDII
                     SRQVLTLRSISGYDVPLLFMNSFSTEEDTLKYLEKYPDLGKQGLPLSFLQNKFPRIRQ
                     DNLMPYENKDKKLMWNPPGHGDIYTALGDLLEKMIMQGCRYAFVSNADNLGAIVDTSI
                     PAYMEDNNIPFLMEVCLRSPMDKKGGHLCEDKSGQLLLREIAQCSDNDLPRFQDIEYY
                     KYFNTNNLWIDLRALEWYIIANEGLLLLPLIVNPKIVEGTPVYQLETAMGSAISIFNN
                     SKALIVPRERFAPVKKTNDLLALWSDAYDLNDMYQVVLKRGLEKPPVIELDEIYYGKI
                     DDMQKRFSKGIPCLSGCKELKIYGDVSFGEGVICEGKTTLKASNPVFVQNCLLTGEVN
                     FDMQRNFPQDKAGG"
     gene            complement(3630..5639)
                     /locus_tag="PLB65_06995"
     CDS             complement(3630..5639)
                     /locus_tag="PLB65_06995"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HOQ78311.1"
                     /translation="MVYRKFEVHLTDELPPEPTFEPNIRRAPDRGLDLSKKDIALAVK
                     NALRYVPQKLHSVLAPEFLQELKTRGRIYGYRYRPQGKIYGKPIDEYKGITPAKAIQV
                     MIDNNLDFEVALYPYELVTYGETGQVCQNWMQYRLIKQYLEIMTENQTLVVASGHPLG
                     LFPSRPEAPRVISTNGLMVGKWDNPVEFKRLTALGVANYGQMTAGGWMYIGPQGIVNG
                     TYITLLNAGRKYLGIPENKNLAGVLYVSSGLGGMSGAQSKAIEIAGGIGIIAEVDKSR
                     IETRFNQGWVSKVSSNLEEIFSWVDEARLHKKALSIAYYGNIVDLLEYIDEHNIKVEL
                     LSDQTSCHVVYEGGYTPVGVTYEEGRKLLAKDIELFKQKVDESLRRHFNVLKSLTYKG
                     SKFWDYGNSFMASVFDAGVTEIARNGINTIDGFIWPSYVEDIMGPICFDNGYGPFRWI
                     CLSRKDEDLHKTDLAAISLIDKNRSAMDKDNHYWISTAEENNLVVGTKARILYADEES
                     RIKLGLKFNEMVRKGEIGPVMLGRDHHDVSGTDSPFRETANIYDGSNLMAEMATHCFA
                     GNIARGMTLVVLSNGGGVGVSRCINGGNGIVLDGSERMDEVVKNGLSWDVMGGIARRA
                     WAQNEGAIKTSNAWNEKHSSEGNITIAEKVDEEMVEHLVKKEFGE"
     gene            complement(5652..6932)
                     /locus_tag="PLB65_07000"
     CDS             complement(5652..6932)
                     /locus_tag="PLB65_07000"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HOQ78312.1"
                     /translation="MSETENILALLKQEVSPAMGCTEPAAVALAGAYAASVIDETIIS
                     ADLIVSPNILKNGMGVGIPNTNLLGLDIAFALGILVAKPEKELEVLADLDEKTIDAAK
                     RMLDEQKVNTTMKETEEKIWIEANLKTSKSESTAILRWRHNWLFHMEKDKKILCHNEL
                     GADKGPRLVSPASYILKDYYDFCINIEIDKLESIDLGLHLNRKIADFGLSNKSGINVG
                     KMIMDQIKSGLLGDDIHHYAMALTAAATDARMSGCNMPVISNSGSGNQGLSITLPIIA
                     VGEKLSSSREELIRALAMGHLVSIHIKEKIGILSCLCGCLSASAGAASGIVMLLKGDY
                     QQIEYAIKNMIADTAGMVCDGAKEGCSLKVATTTSAAVQAALLAKAGICVSSNDGIIT
                     ESIEGTIENLAYFVTTGMQDADSTILNIMLNKTK"
     gene            complement(7262..>8019)
                     /locus_tag="PLB65_07005"
     CDS             complement(7262..>8019)
                     /locus_tag="PLB65_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1848 domain-containing protein"
                     /protein_id="HOQ78313.1"
                     /translation="SKNPSPMLRKLSYFDEKGYNYYFQFTLNDYEQEKWEKNLPALNQ
                     RLENFIELSELIGNQKVIWRFDPFIISDKLHPEELLKRIENIGDQIYAYTNRLVISFI
                     DIDIYKRVKRNLSALPDEIKEIDIPTMNYFARELMRLNQKWNLKIATCAEQIDLHQYG
                     IEHNRCIDDRLIIKLFSDDTELMNFLGYKPTEQTDFLIGQNENSFYSYKKLKDKGQRK
                     FCGCIKSKDIGQYNTCSNGCVYCYANSKRKIDS"
BASE COUNT         2351 a         1734 c         1214 g         2720 t
ORIGIN      
        1 ctctgctttt ggattcgtcc ataatcacaa tttttaattc gccccaattg ggtttaacca
       61 ctgttctttg acgaggtaaa atctgaacag ggataattag atcttccatt tccccaatta
      121 ttactctata tctacccgga cgcagtaaag ctggcactcc cattactagt tcatctgtag
      181 agataacaga attcagcgaa tccaattctt cgattgtata acggagctct tcttcagttt
      241 gggttttagt aataaccatc aactccccat aatcattaac agtttctgag gcaaacattt
      301 cttcgtaaac atatctagcc attggtgaag cagaagtagc aagagatttt tcatctattt
      361 tgtggccact gaagtacaga ttacatttgg ctgcaaagag atctgtctct tccataatca
      421 tatcgttaaa tgcttgaacc aaatatatca tagaggggtc tatcagctct actgaacgtt
      481 catttttatg cgataaaact atccgctcag aagtggaatc ccgtaaaaca aattccattt
      541 ttaaataagc tcggggctcg gcaccttcta tcttttcaat attatacact gtcgtttcca
      601 gaaaatagtg aggttcttta tccaaaattt ccccgcggga tacagttcca aaaatattgt
      661 atgctttcac acgctggaca actaagttgg gaatagcatc tcgtagcctt gttgcccaaa
      721 catcagtctg caggaatttt actttataaa aattctcttt taccactatc tgattacggt
      781 cataagttct gggcaaaact gtatccagca cttccagatt ttttcctgta ttattagctc
      841 tgcgtaattc aggtttttca cttcctggtt gatattccaa aacatagtag tttacaggtg
      901 ttttttccac cttttgcaag cagcctgaaa gcataattac caaagctaaa catcctataa
      961 tgaagcacat actctttatt ttatgcataa taactcctaa tttccgcgta gcaagatagc
     1021 aggcgtatcg gcaatctgac gagaaaattc attcagattt tcactcgtct cggttagggt
     1081 gcttaaaatt tccagtaagt ctccctgacc acgcgttaca actctattca agttcgcaac
     1141 caaatttcct gttttatgaa ttgtagtgga tagctcactt accatttcat taatattagt
     1201 tctagaaagt tctccgcaca gagtccccag gttagtcatt aaactgtcta ctcctggagt
     1261 ataaagaata gcattcacct ggtctaatgt cttattaata ttattactga tcgttaatat
     1321 ttcgcgggta gtttccaaaa tcatttgatc gctatgttca cctactgtag taacttttaa
     1381 attagcctcg cgaatcaaag cagagccatc ttcagttaac cttgccaagc tatattctag
     1441 ttgagaagtt aattcctcca actgtttatt tacattacta gtaacgctaa ttaaacttgt
     1501 ctgcgaggtt tccgaaacac tttgaatatt gcttgtagcg ctattagtga tagcatcaag
     1561 attcttagtg agattatcgg ttactgcact taaatctcta cttagttcct ctgttaaatc
     1621 cgctgtatta tttgctattc tactaatgct ttgcatcgta gtagccagat tttcgtgagt
     1681 atctttcaaa atcaaacttg tttgctcgag gatcttagaa atattctgcc tgttttcctc
     1741 tccagtaatt aagtttaggt tggcagctat catatcgatt ttatccacaa ttgattttgc
     1801 ccgttcactg atatcttcca acattgtagt tccaggcttt atataacttt taggtttcaa
     1861 cagtttagct tcattagttc cgccccgaat ttccacagat ttcagcccag ttattcccga
     1921 taacagtaaa acggcttccg tatcttcttt tataggggtt ccccggttaa cacttataga
     1981 gacaattatt ttagttacat ccttaggatt gattttaata tcttctactc taccgatttt
     2041 aataccgcgg taattaaccg aactgccaac ttgcaatcca ctaacagaat agtcctcaaa
     2101 ttcaatataa tatatatctt tcttttctac taatctgcta ccagctacaa ttgccgcaaa
     2161 aattataatc agaattgccc cgatagttaa aaaaacccct agtcgtattt tagcagcctt
     2221 ggaaaccata ttcatcctcc tgctttgtct tgagggaaat ttctttgcat atcaaaattc
     2281 acttctccgg taagcaaaca attttgcaca aataccggat tactggcttt tagagttgtt
     2341 ttgccctcac aaataactcc ttctccaaaa cttacatccc cgtaaatttt caattcctta
     2401 caaccgctta aacaagggat acctttgcta aaacgctttt gcatatcatc aattttccca
     2461 taatagattt catccaattc aattaccggt ggtttttcca gaccacgctt caacactacc
     2521 tgatacatat cgttcagatc ataagcatcc gaccaaagag ctaaaagatc attcgttttt
     2581 tttacaggtg caaatcgttc cctgggaaca attaatgcct tggaattgtt aaatatacta
     2641 attgccgaac ccatagctgt ttccaattga taaacaggag ttccttccac tatcttagga
     2701 ttaacaatta acggtaacag cagcaaacct tcattagcaa ttatatacca ttctaaagct
     2761 cttaaatcta tccacaagtt attagtattg aaatatttgt aatattcgat atcttgaaag
     2821 cggggcaaat cattatcgga gcattgtgca atttccctta agagcaattg tccagattta
     2881 tcttcacaaa gatgacctcc ttttttatcc attgggctgc gtaaacagac ctccattaaa
     2941 aaaggaatat tattatcttc catataggct ggaatggatg tatctacaat cgctcctaaa
     3001 ttatctgcat tggaaacaaa agcatagcgg caaccctgca taatcatttt ttccagtaaa
     3061 tcacccaaag cagtataaat atccccatgc cccggagggt tccacatcaa tttcttatcc
     3121 ttattttcat agggcatcag gttatcctga cggatgcggg gaaatttatt ctgtaaaaaa
     3181 gaaagtggca aaccctgctt acctaaatct ggatattttt ccagatattt tagggtgtcc
     3241 tcttcagtac tgaaactgtt cataaaaagc aagggaacat catatccgga aattgacctt
     3301 aaagttagaa cctgacggct gataatatcc agaaagttca gattgttttt cactggtaat
     3361 agtgatttcg ctttggaaag ccccatactt gttcctaagc caccatttag ttttataaca
     3421 gcagtatttt ttaacataga gctgcgagaa ttttgtctta tctgttgata atcaatcaaa
     3481 ttctcttccg ctggaggaga aatttcttct tccttaataa gccctttttc gcctttaaca
     3541 agcatcttat aatatgcttc aaaagtacgg ataacatctt cccttatgcc ttcctgtttc
     3601 atcaaccgga taaagggagt gctcattttc tattctccaa attccttttt tactaaatgc
     3661 tccaccattt cttcatctac cttttctgca atagtgatat ttccttcaga gctatgtttt
     3721 tcgttccaag cgttacttgt tttaattgct ccttcatttt gagcccatgc ccgtctggca
     3781 atccccccca ttacatccca agagaggcca tttttaacaa cttcgtccat tctttcactg
     3841 ccatctaaaa cgattccatt tccaccattt atacaacggc ttactcctac tccacctcca
     3901 ttggaaagaa ctaccaaagt cattcctctg gcaatatttc cagcaaagca atgtgtagcc
     3961 atttcagcca ttaaatttga gccatcatat atattagctg tttcgcggaa aggagaatcc
     4021 gttccagaaa catcatgatg atctctacct agcataacag gaccaatttc acccttgcga
     4081 accatttcgt taaacttcaa ccccagtttt attctgcttt cttcatcggc ataaagtatt
     4141 cttgctttgg ttcccaccac caggttattt tcttctgcag ttgaaatcca atagtgatta
     4201 tctttgtcca tagcgctgcg atttttatct attaaagaaa tggcagccaa atccgtttta
     4261 tgcaggtctt catctttgcg ggaaagacaa atccacctga aaggtccata gccattatca
     4321 aaacaaatag gtcccataat atcttccaca taagaaggcc agataaaacc atctatcgta
     4381 tttataccgt tgcgggcaat ttctgttaca cctgcgtcaa acacacttgc cataaagctg
     4441 ttgccataat cccagaattt tgaaccctta tatgttaaac tttttagtac attaaaatgt
     4501 ctccttagag attcatcaac tttttgcttg aataactcaa tatcttttgc caaaagtttg
     4561 cgcccttctt cataagtaac gcccacagga gtatagccac cctcgtaaac tacatgacac
     4621 gaggtctgat cggaaagcaa ttccactttg atattatgtt catcgatata ttccagtaaa
     4681 tctacaatat tgccataata agctatagaa agagcttttt tatgtaagcg tgcttcatca
     4741 acccaactaa atatttcttc caaattactg gagaccttgc taacccaacc ctgattaaat
     4801 ctggtttcta tcctgctttt atctacttca gcaattatac caatgccacc tgcaatttca
     4861 attgccttac tttgagctcc actcattcct cccaaaccgg aagaaacata taaaactcct
     4921 gccaagttct tattttccgg aatacccaga tattttctac ctgcatttag cagggttata
     4981 taagttccat tcactattcc ctgtgggccg atatacatcc atccgccagc tgtcatctga
     5041 ccataatttg ccactcctaa tgcagttagc cgtttaaact ccactggatt atcccatttg
     5101 ccaaccatca aaccattggt agatattact ctaggagctt ctggacgcga agggaacaaa
     5161 cccaaaggat ggcctgaagc tacaaccagt gtctgatttt ctgtcataat ttccaagtat
     5221 tgctttatta accgatattg catccaattt tgacaaacct ggcctgtctc tccgtaggtt
     5281 accaattcgt aaggatataa ggcaacctcg aaatccaaat tgttatcaat catcacttga
     5341 atagctttag caggggttat acctttatat tcatctatcg gttttccgta aattttgcct
     5401 tggggtcgat agcgatagcc atatattcta cctctggttt ttagttcctg taaaaattct
     5461 ggtgctaaaa cgctatgtaa tttttgagga acatagcgaa gtgcgttctt tacagccaga
     5521 gctatatctt ttttgcttaa atccaatcct ctatctggag ctctacgaat attaggttcg
     5581 aaagttggtt ccggaggcag ctcatcagtt agatgaactt caaattttct atataccatt
     5641 ttttatctcc tttattttgt cttattcaac attatattaa ggatagtgct gtcggcatct
     5701 tgcattcctg ttgttacaaa ataagcaaga ttttcgatag taccttcaat gctttctgta
     5761 atgatcccat cgttggaaga aacacaaatt cctgcttttg ctaatagagc agcttgtacc
     5821 gcagcacttg tagtagtagc aacttttaat gaacaacctt ctttagctcc atcacaaacc
     5881 attccagccg tatcggcgat catatttttg atggcatatt caatttgctg ataatctcct
     5941 ttcaaaagca taactatacc actggcagct cctgcagaag cagaaaggca tccgcacaaa
     6001 caagatagaa tcccaatctt ttctttaata tgaatactta ctaaatgacc catagctaat
     6061 gctctgatta gttcttccct ggagctacta agtttttcgc caactgctat tataggaaga
     6121 gtgatggata aaccttgatt tccacttcca gaattactaa taacaggcat attacaacca
     6181 ctcattctgg catcagtagc agcagcagtt aaagccattg cataatgatg aatgtcatca
     6241 cccaaaagac cgctttttat ttgatccatt atcattttac ctacattaat gccagattta
     6301 ttggatagcc cgaaatctgc tatcttacgg tttaaatgta aacctaaatc aatagattct
     6361 aatttgtcta tctctatatt aatgcaaaaa tcatagtagt ccttcaagat ataagatgca
     6421 ggagaaacta aacgaggtcc tttatcagct cccaattcgt tatggcataa aatcttttta
     6481 tctttttcca tatgaaacaa ccagttatgc cgccaacgca aaattgcagt actttcagat
     6541 ttggaggttt ttaaatttgc ttcaatccag atcttctctt cagtttcttt cattgtggtg
     6601 ttcacttttt gttcatctaa cattcgtttt gcagcatcta tcgttttttc atcaagatca
     6661 gctaaaactt ccagttcctt ttctggttta gccaccaata ttcctaaagc aaaagcaata
     6721 tccaaaccga gtaaattcgt attgggaatc cctactccca tcccgttttt cagaatattg
     6781 gggctaacaa ttaaatctgc agaaataatt gtttcatcta taacacttgc agcataagct
     6841 ccagctaaag caacagcagc tggttctgta catcccatag caggagaaac ttcttgtttt
     6901 aatagcgcta agatattttc agtttcactc attatcgatc ctaaaactaa tttttaattt
     6961 tttactattc tttatatcgg atttattccg tcaagcaaaa aaggaagttg atattatgat
     7021 ttcaggatta tttcataccc aaaccaccga attaaaccag aacaacaaaa tagattttac
     7081 acacccagat tgcacaggat aataggtctg ggataaacaa gtttaagaga atttatagga
     7141 ttttatttta tgcaggactc tttaatttag aagcattaaa catatatcca gtcttccaat
     7201 ttgtctggac ttgcaaatat ataagtgatc tgaaatcccc tctttttacc tcacttatta
     7261 ttcaactatc aatctttctt ttagaattgg cataacaata tacacaacca ttagagcagg
     7321 tattatattg cccaatatct ttgcttttta tacaaccgca gaatttgcgt tgtcctttat
     7381 ctttcaattt tttgtagcta tagaatgaat tttcgttttg tcctattaaa aaatctgtct
     7441 gttcagttgg cttatagccc aaaaagttca ttaattcagt atcatcagag aaaagtttaa
     7501 taatcagacg gtcatcaata cagcggttat gttcaattcc atattgatgt aggtctattt
     7561 gttcagcgca ggtagctatt tttaagttcc atttttggtt tagtcgcatc aattcccgag
     7621 caaaataatt cattgttgga atatctattt cttttatttc atcgggtaat gcgcttaagt
     7681 tgcgtttcac tcttttgtat atatctatat ctatgaagct gataactaat ctatttgtat
     7741 atgcataaat ttggtcacca atattttcta tgcgttttaa gagttcctcg ggatgtaatt
     7801 tatcactgat aataaaggga tcaaaacgcc agataacttt ttggttacct ataagttcag
     7861 atagttctat aaagttctcc aggcgttgat tcaaagctgg tagatttttt tcccattttt
     7921 cttgttcata gtcattcagg gtaaattgaa aatagtaatt ataacccttc tcatcaaagt
     7981 aagacagctt tctcagcata ggagaaggat ttttgctcc
//
LOCUS       DAOSTH010000071         7678 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_8958_length_7678_cov_14.539551, whole genome shotgun sequence.
ACCESSION   DAOSTH010000071 DAOSTH010000000
VERSION     DAOSTH010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7678)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7678)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7678
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8958_length_7678_cov_14.539551"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="PLB65_07010"
     CDS             complement(<1..883)
                     /locus_tag="PLB65_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78314.1"
                     /translation="MKKPVAQCRHFIFLLAIAICASINLYADWRIKLTITNQGSDDSI
                     SASVFLGESEGAADGLDTYDFPLGPPPNNLPYIRAYFPHNDWGSTFNGNYIKDIRSIN
                     PAAKTWNFTFSKSGTPLSNNYRLSWNITGLFPACYQPRLVFKSENINMLTQSSYSYTG
                     WVTSGSIQMQYNNAMPYQVLTLQELLFSDNLTKGLNLNRYFKVGSGTLNFNCHPNANL
                     VQNLVTLQDSVWLNIYPVAGFVGTTIAEISVIGSGGTLNAQISVIRDATNSPPVYLDA
                     IQPLPIIQNQQGIFSWAG"
     gene            1509..5393
                     /locus_tag="PLB65_07015"
     CDS             1509..5393
                     /locus_tag="PLB65_07015"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ78315.1"
                     /translation="MSTSKLLPGMVQWTLEQKNPDNVSCSFGCGYLFLDVCGFTRLTE
                     TASAKGHYGVEVITNLLNEYFDLLNSQIMRAGGQIIKFEGDAVLAAFPDSENACHITI
                     KKCVEDIKQGLKKLNKRLESTYGSDLAFHSSMGFGISNIVILGKNKKHWDYFVYSPIL
                     ASLYTQCDIAGKNECLETNVLIPANDQYSYNHNQIPQSSELSYDHLFFPPEVLQRISS
                     ESFSGELRNAAILFIGINAEILIRKSDYKTLNKYYSSVQDIVYRLGGMINKIDYTDKG
                     LILLISFGILQTHIDDIERAIICANQINKLKSPLKAKIGLTYSNLYAGILGGQQRYEF
                     GIIGNGVNVAARLMTSAKYGQIVFTKDILPSVQSRFEVQFLRKDKVKGIKGELEFYHI
                     VRELPEYLNSYKKQYDDKTQVFYQNETESIIKKIASKKLSQLLISGEHGTGKSFISWK
                     IISSFYAKASKIAIIVLDEFNRHDFLILHRKFISKSLGIDDPLAEPDKLKEYLYNLMD
                     KRDSDILLQALGLQNQGSKQIIDSEKHKDLVLLSLYKSLYILMKDLDIVLLDNIQWLD
                     DLSNQIIQKRLEEETIITPILIMTSTDDFKHNKSNRSAKTAYLKLDNLDYQSSFELIR
                     SQLSNITHQAVDYILNTAGGNPRFIVELCAQIRSQFPNPAMLITESDIRDIQNRGLLP
                     LSVENLFMIKYDSLSADAKDMLKKASIIGKGFTLDEIVETQKSINQNEIVPIINELQK
                     KEIIDITELSPEVQYLFNNVLMRQAIYSTILLSEKVSLHNRVALFYEEKYAGWTSQHS
                     ELLAYHFHLGENKEKALHYSLLAAEQNYKISNYGEAIYYYQIALQNTDDSEQLNSIIL
                     AIIDSQLYLGEVDKAKMYLENIQVLEIRNADEISKYHFLRLRIYYLEGNYEEVLNYLS
                     QINFYTGIYGDQIQLYQLDCLYKLFRIDEFSTLMKKLKQEYLKQAATLLKDKQQKTTL
                     TSLLNIYKQIPDEKTTAEQRHYLYLLLKLEAIQANHFLNTGQYKEAVTSLKFQYNLAK
                     ALKDDLSMRIACGGLGIVYTRMGKLDEAHQAYIEAINIADKISDRFGYAKVLADMATL
                     HRRMGNHKQALDNFQRSLKIFESLGNLVLQGFVLHGIGEVLIQEEKYDESLVYFRKAL
                     KIAKQCNDLFGISFEQDAIGDILFQNGKITEAKTLYHKNLKLQKKIGDTEGIAHTYGN
                     LGNVARAENNLPLAMEYYNKNIAMTAEIGDLDGNGRGYYNLAGIYEIMKDKDKFVELL
                     NKSIECFQKAGSVQYLELAKQRLQEFERGSEV"
     gene            5830..>7678
                     /locus_tag="PLB65_07020"
     CDS             5830..>7678
                     /locus_tag="PLB65_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOQ78316.1"
                     /translation="MNAYYRQPAIYKDQVVFIAEDDLWTFSLKDEVARRLTANLGMIR
                     NPQFSPDGKQIAFTSTEEGTSEIYIMPSEGGEAKRLTYLGAYLYTLGWKGSKIIFASS
                     HSSYLQGYYKLYEIDCEGGAPVELPYGRATDISFGEKGVVLGRNTGNPARWKRYRGGT
                     AGYILIDKNGDGKFKKLIDLKGNFAHPMWIGNRIYFICDHEGIGNIYSCTPEGKDLQK
                     HSQHKDFYARGAYSDGKNIIWHAGAELWLMDVKENKPRKLSFAYSSPMVQRQRKFVSP
                     PKNLENVYLSPDGGSVSLELRGKALVSGNWEGSVQQYGWHQGVRYRLPQILPDGKHLL
                     LLSDEPGYERFEIHPLRKDFEKVTEEVVIIDNPDLGRVTSFVPAPKGSLVAIENHRCE
                     LIIMDLESKEFKTIDRNEFGLIWELNWSPDGRWLTYSNNRDRNTRHIIIWDSKENQLH
                     IVSKPVLNDTAPVFDPEGKYLYFVSVRNLNPLPDGIQFDFIYPNSYKPYLIPLKKELR
                     SPFIQEPKPFEAKPETPKKDEKKEEKEPKDEIKPVEIDFDGIYDRVIEFPVSSGMYGG
                     LSAIKDRLFYVNMPLENRAEDDNKFDLMCYDLNKLESWTYAKDITGYHIC"
BASE COUNT         2617 a         1288 c         1465 g         2308 t
ORIGIN      
        1 ttcccgccca ggaaaaaatt ccctgttgat tttggattat cggtaagggt tgaatagcat
       61 ccaaatagac aggtggcgaa ttagtagcat cccttataac agaaatttgt gcattaaggg
      121 ttcctccact tcctattaca gatatttccg ctattgttgt tcctacaaaa ccagctacag
      181 gataaatatt aagccacaca gaatcctgca aagtaaccaa gttctgaacc aaattagcat
      241 taggatgaca gttgaaatta agcgtgccac tgcccacttt aaaatatctg ttcaggttaa
      301 gaccctttgt aagattatcg gaaaaaagca attcctgcaa agtaagaact tgatacggca
      361 ttgcattatt atattgcatt tggatggaac cggaagtaac ccaaccagta taagaataac
      421 tactctgagt aagcatattg atattttcgc tcttaaaaac taaacggggt tggtaacaag
      481 cagggaaaag tcctgttata ttccaagaaa gacgatagtt attacttaaa ggagtaccgc
      541 ttttagaaaa agtaaaattc caggtttttg ctgcgggatt tatagatctg atatctttta
      601 tgtaatttcc attgaaagtt gagccccaat cattgtgagg aaaataagca cgaatgtaag
      661 gaagattgtt aggtggaggt cctaaaggaa aatcatatgt atctaaacca tctgcagcac
      721 cttcactttc acccaaaaat acagatgcac ttattgagtc gtcagaaccc tggttagtaa
      781 ttgttaattt tatccgccaa tcagcataca aattaataga tgcacaaata gcaatagcta
      841 ataaaaaaat aaaatgcctg cattgcgcaa caggtttttt catataaacc tctttccgac
      901 cgaatgttat acatatctct tgaattacta tttgcttttg ttgtcaagtt attttttatg
      961 cctaataaaa taatattatt ccaaataagg tagttaatta caaataaaga agatgctaaa
     1021 taaataacta ctcaattaaa tttaatttca tagtcaagag aattagcaag aattcccttt
     1081 tcattattaa gtaaaacttg taccttgctt aattggatgt cgtgcaattg tcgtggtgct
     1141 actaggcaaa tttacagaaa acacaacctc agcactacca tagataaaca agcaagcaaa
     1201 ttgcaaaact gctcgtttgg tttattattt tttttaagaa gtgtacagaa tcagatgtta
     1261 agtttatata agcatatata gttgtatata gcgattctga ggccactgga ttaaattttt
     1321 agataaccca agatcagcat tatgggatat ttagattttt taattagagt gcagaacaga
     1381 taatctatac gcattcacag gattagacaa tatcaagtat acttacctta aacactacaa
     1441 tatttttctt gacttatttt aggtattata taaaaaaagt aagattgact taaaggggga
     1501 aagtgatagt gtctacaagt aaattactgc ccggaatggt tcagtggact ttagagcaaa
     1561 aaaacccaga taatgtctcc tgtagttttg gttgcggtta ccttttttta gatgtttgtg
     1621 gatttacaag attaactgag acagcttctg cgaagggtca ttacggtgtt gaagtaataa
     1681 ccaacctatt aaatgaatat tttgatcttt taaatagcca gataatgcgc gctgggggtc
     1741 aaataatcaa gtttgaaggt gatgctgttt tagcagcttt ccccgattca gaaaatgcct
     1801 gccatattac aattaaaaaa tgcgtggaag atatcaagca ggggttaaag aaattaaaca
     1861 agcgcttaga aagtacttat ggaagcgatc ttgcctttca tagtagtatg ggttttggta
     1921 tctccaatat agtaattttg ggaaagaaca aaaagcattg ggattatttt gtttacagtc
     1981 caatcttagc atctttatat acgcaatgcg acatagctgg aaaaaacgag tgtttggaaa
     2041 caaatgtgtt aataccagct aatgatcagt attcatacaa ccataatcaa attccacaaa
     2101 gttcagaact tagctatgat catctatttt tccctccaga agttttgcag aggatatcct
     2161 cagaatcttt tagtggtgaa cttcgtaatg ctgctatttt gtttatcggc ataaatgcag
     2221 aaatattaat ccgtaaaagc gattataaga ccctgaataa atattattca tcagttcagg
     2281 acattgttta tcgtttgggt ggaatgatca acaaaattga ctataccgat aagggattaa
     2341 ttttgctgat cagttttggc attttgcaaa cacatattga tgatattgag cgagctatta
     2401 tttgtgcaaa ccagatcaat aaactgaaaa gtccattaaa agccaaaata gggctcactt
     2461 atagtaatct ctatgcggga atattaggag gacagcaacg ctacgaattt ggcataatcg
     2521 gcaatggagt taatgttgcc gctcgcttga tgacttctgc aaagtatggt caaattgtgt
     2581 ttacaaaaga tattctcccc agtgtacaaa gccgttttga agttcaattt ttacgaaagg
     2641 ataaagttaa gggcattaaa ggggaactag aattctatca tattgtgcgg gaattacctg
     2701 aatatctaaa cagctataaa aagcaatatg acgataaaac tcaggtattt tatcaaaatg
     2761 agaccgaaag cattatcaaa aagatcgctt ccaaaaagct ctctcaattg ctaatctcag
     2821 gagagcacgg aacaggtaaa tcttttataa gctggaaaat catcagcagt ttttacgcta
     2881 aagccagcaa aattgcaata atcgttttgg atgaattcaa ccgtcacgat tttctcattt
     2941 tacacagaaa gttcatcagt aaaagcttag gtattgatga tccacttgct gaacctgata
     3001 aattgaaaga atatctatat aacctaatgg ataagcgcga ttctgatatc ctgttacaag
     3061 cgctcggttt acaaaatcaa ggctctaagc aaattattga tagtgaaaaa cacaaagacc
     3121 tcgtgctgct ttctctgtat aaatcactat acatcttgat gaaagatctt gatatcgtcc
     3181 ttttggataa cattcaatgg ctggatgatc tttctaatca aatcatacaa aaacgcctgg
     3241 aagaggaaac aattataacc cctatcctga taatgacatc aacagatgat tttaaacata
     3301 acaaaagtaa tagatcagcc aaaactgcat acctaaaact ggacaattta gattaccaaa
     3361 gcagttttga gctaattcgt tcgcaattat ctaatatcac acatcaggca gttgattaca
     3421 tcctaaatac ggctggagga aatccgcgtt ttattgtgga actttgtgct caaattcgtt
     3481 ctcaatttcc aaatccagca atgctaatta cagaatctga tattcgtgat attcaaaata
     3541 gagggttact tcccttaagt gtagaaaatc tgtttatgat taaatatgat tcacttagtg
     3601 cagacgccaa agatatgcta aaaaaggcat ctattatagg aaaaggtttc actttggatg
     3661 aaatagtgga aactcagaaa agcatcaacc aaaatgaaat agtccctatt ataaatgaac
     3721 ttcagaaaaa agaaataatc gatatcacgg aactatctcc cgaagttcaa tatcttttta
     3781 ataatgtatt gatgaggcaa gctatttatt ctacaattct attaagtgaa aaggtgtcgc
     3841 tacataatag ggtagcttta ttttatgaag aaaaatacgc aggctggact tctcaacaca
     3901 gtgagctgct tgcctaccat tttcatttag gtgaaaataa agaaaaagca ctgcattatt
     3961 cactgctggc agctgaacag aattataaaa ttagtaacta tggagaggca atctactatt
     4021 atcaaatagc tctgcaaaat acagatgatt ctgaacaact gaattccatt attttggcta
     4081 ttatagattc tcagctttat ctgggagaag tggataaggc aaaaatgtat ctggaaaaca
     4141 ttcaagtgtt ggagattagg aatgctgacg aaataagtaa atatcatttt ctccgtttaa
     4201 gaatttatta cttggaagga aattatgaag aagtgctgaa ttatctatct caaataaact
     4261 tctatacagg aatatatgga gatcagatac aactttatca gctagattgt ctttataaac
     4321 tgtttcgcat agatgaattt tctactttga tgaaaaaatt aaaacaagaa tacctaaagc
     4381 aagctgctac acttctgaag gataagcaac aaaaaactac acttacatct ctattaaata
     4441 tttataaaca aatccctgat gaaaaaacaa cagcagaaca aagacactat ctatatctct
     4501 tgctaaaact ggaagcaatc caagcaaacc attttctgaa cacaggacag tataaagaag
     4561 cagttacttc gcttaaattt caatataatc tggcaaaagc tctgaaggat gatctttcta
     4621 tgcgtatcgc ctgtggtggt ttaggaattg tttataccag aatgggaaaa ttagatgagg
     4681 cacatcaggc atatattgaa gcaatcaata ttgccgataa aatcagtgat cgctttggct
     4741 atgcaaaagt acttgccgat atggcaactt tgcatagaag aatgggaaat cataaacagg
     4801 cattggataa tttccagcgc agcttgaaga tatttgaatc tctgggaaat cttgtgttgc
     4861 aaggttttgt gttacacgga attggcgaag ttcttattca agaagaaaaa tatgatgaat
     4921 ccctagtata ttttcgcaaa gcactgaaaa tagctaaaca gtgcaatgac ctctttggta
     4981 tttcatttga acaggatgct attggcgaca ttctgtttca gaatggaaaa attacggaag
     5041 caaaaacact ctatcacaaa aatttgaagt tacaaaaaaa aattggcgat acggaaggga
     5101 tcgctcatac ctatggaaac ctaggaaatg ttgccagagc agaaaataac cttcctctcg
     5161 ctatggaata ttataacaaa aatatagcta tgacagctga aataggagat ctggacggaa
     5221 atggaagggg ctactataat ctagcaggta tctatgaaat tatgaaggat aaagataaat
     5281 tcgtagaatt attgaacaaa tccatagagt gctttcaaaa agcaggttcg gtacaatatc
     5341 tggaattggc taaacagaga ctgcaggaat ttgaaagggg gtctgaagtc taaaggattt
     5401 gggatctgga tgtaaatctt gctggtaatt ttcttcctcg ttaaccaccc aaaaaatact
     5461 cgtgaaatta gttggaagat ctcggaaact ttataactct gcctccagga aacgaagaag
     5521 atatcgttct tcttgtaaat cagaaagcgt tttgataact ttgtctttgc ctataacttg
     5581 aaaaaccccc aactttatga cagctttatg tgttttattt attaattaca atcgttatga
     5641 gccatctata ctaaacaaat tataggaatg cgtgaaagtt actttggcag aaacgatttt
     5701 agtttcttcg atgtccttat ttttgatact ttttttagat taaccttatt atatatttac
     5761 ttgacttaaa tatccactat gaaaagagtg caaaataata aagtaagtta ttattaaagg
     5821 agcatcctta tgaacgcgta ttatcgtcaa cctgccattt ataaagatca agtggtcttt
     5881 attgctgaag atgacctctg gacttttagc ttaaaagatg aagttgccag gcgcttaact
     5941 gccaatttag gtatgattcg caatccccaa ttttctcctg atggtaagca aattgcgttt
     6001 accagtacag aagaaggaac ttccgaaatt tatataatgc cttccgaggg tggcgaagca
     6061 aaacgcttaa cttatttggg agcttatctt tatacacttg gatggaaagg cagcaagatt
     6121 attttcgctt cttctcacag ttcttatctc caaggttatt acaaacttta cgaaattgat
     6181 tgtgaaggcg gagctccggt tgaattacct tatggaaggg caaccgatat ttcttttgga
     6241 gagaaaggag ttgttttagg tagaaataca ggcaatcctg caaggtggaa aagatatcgg
     6301 ggtggaactg ccggctatat tttgattgat aaaaatggcg atggcaaatt caagaagctg
     6361 attgacctga aaggaaattt tgctcatccg atgtggattg gcaaccggat ttattttatc
     6421 tgtgaccacg aaggtattgg caatatttat tcttgcaccc cagagggcaa agacctgcaa
     6481 aaacacagcc agcataagga tttttatgcc cgcggcgctt atagcgatgg aaaaaatatt
     6541 atctggcacg cgggagcaga gctttggttg atggatgtta aagaaaacaa accccggaaa
     6601 ttgtcttttg cttatagcag tccgatggtt cagaggcaac gcaaatttgt tagtccgccc
     6661 aaaaatctgg aaaatgtata tctttcacct gatggtggtt ccgtttcttt agaattacgc
     6721 ggaaaggcat tagtaagtgg aaattgggaa ggcagtgtgc aacaatatgg ttggcatcaa
     6781 ggagtgcgtt atcgtctgcc ccaaattttg ccagatggaa aacatctgct attgctgtct
     6841 gatgaacctg gctatgagcg ttttgaaatt caccctttac gcaaggattt tgaaaaggtt
     6901 accgaagaag tagtgattat tgataatccc gatttgggca gagttacatc ttttgtccct
     6961 gctcccaagg gttctctggt ggctattgaa aaccaccggt gcgagctgat tattatggat
     7021 ttggaaagca aagaatttaa aaccatagac cggaacgaat ttggtctaat ttgggaactt
     7081 aattggtctc cggatggacg ctggctaact tatagcaata atcgtgaccg caacacccgg
     7141 catattatta tctgggatag taaagaaaat caattgcata tagtaagtaa acctgtgtta
     7201 aatgataccg ctcctgtttt tgatccggaa ggaaagtatc tctattttgt gtcggtgcgc
     7261 aatttaaatc ctttaccgga tggcattcag tttgatttca tctaccccaa ttcctataag
     7321 ccctatttaa ttccgctgaa aaaagagctg cgctctcctt ttattcagga accgaaaccc
     7381 tttgaagcaa aaccggaaac ccctaaaaaa gatgagaaaa aagaagaaaa agaacccaaa
     7441 gacgaaatta aaccagtaga aattgatttt gacgggatat atgatcgcgt aatagaattt
     7501 cctgtttcgt ccggaatgta tggtggtttg agcgcgatca aagaccgctt gttttatgtg
     7561 aatatgcctc tggaaaacag ggcggaagat gataacaagt ttgatttgat gtgctatgat
     7621 ttgaacaaac tggagagctg gacttatgcc aaagatatta caggttatca tatctgcc
//
LOCUS       DAOSTH010000072         7521 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_9171_length_7521_cov_9.621082, whole genome shotgun sequence.
ACCESSION   DAOSTH010000072 DAOSTH010000000
VERSION     DAOSTH010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7521
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9171_length_7521_cov_9.621082"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            430..1584
                     /locus_tag="PLB65_07025"
     CDS             430..1584
                     /locus_tag="PLB65_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78317.1"
                     /translation="MKQFLVVVMLFSCIGLAAIGEGAFLLLRFEPSNINRGMGEITGV
                     VNIWHNNPFTAYSNPAISSFQKGFAYSYAHEDWIDFGSDSKLEYNRALMTIAYNGFAI
                     TLPGYNSDQRWGISMPITYTLYPFTDVNIEDYTQIRAFGLAINPCEIFRNNHLETYPW
                     LKYIDLAIGANYIPLKDEVTYIIYDEDIDDYQMEKMKVDADVLNIGEILRLKYCFQDK
                     LALEAVYGLTHFNANAEHVKYDNDTEAEPIYADRNQGFAFSAAVKTNPILVNTKLHNL
                     GLFDNLFSFRYLNSALVPNYKNSEEITGNGIELGFLDTFYYRSGNYDDDAGGISGSTN
                     GWGINLHYKDLISFGYNYSETPGGDLVDKHKSKEYSCSFDFIGIYDIMVK"
     assembly_gap    2019..2118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2260..3117)
                     /locus_tag="PLB65_07030"
     CDS             complement(2260..3117)
                     /locus_tag="PLB65_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HOQ78318.1"
                     /translation="MLSIIVVPLAAGGISTILLPVAIMGSLGLIFGLVLAFASKVFAV
                     NIDERIEQIIEVLPGANCGACGQPGCAGYATAIVTEDKELNLCAPGGAAVVGKIAKIM
                     GKEASAQQRKIAYIHCSSGGKNNTKWKYDYQGIESCLSAVNIADGPNLCSWGCVGFND
                     CVAACKFDAISVDENGLRIIDRAKCTGCGACVNACPRRLIMLIPESRNVFITCSSKDK
                     NPLPKQNCGTDKPCIGCGLCAKKCPAQAITVENNIARIDYSKCTDCGTCATVCPTKAI
                     IDLKIANRT"
     gene            complement(3137..3739)
                     /locus_tag="PLB65_07035"
     CDS             complement(3137..3739)
                     /locus_tag="PLB65_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit A"
                     /protein_id="HOQ78319.1"
                     /translation="MGFLGQLFTMAISAILIQNFVLSRFLGLCPYLGVSKKMDSAFGM
                     GMAVIFVMTMASAFCYLIYQYLLVPFQLEYLQTLAFILTIAALVQFVEMVIKKNSPAL
                     YKSLGVYLPLITTNCAVLGVAILNITPLADGSRYSFIWAVLNGLFSGIGFTFVLLVMA
                     GIRERLEMVELPKSMRGMPSGLILAGTMALAFYGFMGMKI"
     gene            complement(3739..4329)
                     /locus_tag="PLB65_07040"
     CDS             complement(3739..4329)
                     /locus_tag="PLB65_07040"
                     /inference="COORDINATES: protein motif:HMM:NF009070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOQ78320.1"
                     /translation="MSFIKELTKGIIKENPTFVIVLGMCPTLATSTSVNNAIGMGLAA
                     TFVLICSNIFISLIKNITPDQIRIPVYVIVIAAFVSIVDMSMAAYLPALHKSLGLFIP
                     LIVVNCIIMGRAEAFANKNNVVNSIADGIGMGLGFTLSLSVVATIREILGNGTWLNMR
                     VMPKTYDPMLITLLAPGAFITLGFLMALMNMLKEKQ"
     gene            complement(4326..4916)
                     /locus_tag="PLB65_07045"
     CDS             complement(4326..4916)
                     /locus_tag="PLB65_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HOQ78321.1"
                     /translation="MKIYLQLGLILLAFCLVATALLAYVNTITKPQIDKIKTLEAEET
                     RRTLIPDSDFEEITSDITYYIAKDNKTKEVKGYVFTAEKNGYNGPVKTMVAVDNNFQI
                     IAIKVTDQSETPGLGSNSTQPKFSEQFKGKTSEQLIVDKDGGVPPNCIKAITGATITT
                     RAVVNSVKESIELLKAKVQENSTSSEETKVKEEVNK"
     gene            complement(4913..6016)
                     /locus_tag="PLB65_07050"
     CDS             complement(4913..6016)
                     /locus_tag="PLB65_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOQ78322.1"
                     /translation="MNDKYIVSPAPHFHDKTTTKNVMWNVVLALTPALVFAVYYWGFK
                     SLLLTLLGAVSAMVTEALIQKFRKVPITISDGSAFLTGMLLAYNINAGAPWWIPVLGA
                     AFAIAVGKQVFGGLGNNPVNPALLGRAFLLASWPTIMTSGWVKPKSGSLSGITDLDAI
                     ALNLQSLSPKAYDLVTGATPLKVAQTLRDSSFVSSIITNTDQGVDLANRIFTNLTDLS
                     AIKNLFWGNIGGCIGEVSVFALLLGAAYLLYKNIIEWRIPLYYIGTVFVLSFFLAPLK
                     GSGFSPLLPFFHIFSGGLILGAFFMATDYTTTPITKNGRIIFAIGCGILTMVIRLYGG
                     YPEGVSYSILFMNVLTPLIDKLTMPKAFGWVKK"
     gene            complement(6026..7348)
                     /gene="rsxC"
                     /locus_tag="PLB65_07055"
     CDS             complement(6026..7348)
                     /gene="rsxC"
                     /locus_tag="PLB65_07055"
                     /inference="COORDINATES: protein motif:HMM:NF003454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HOQ78323.1"
                     /translation="MRLKTFPGGVHPPDEKKYSASVPIVDIPIPQKVTIHLSQHIGSP
                     SKPIVSVGDEVLTGQKIAESTGFVSLNQHSSISGKVTAIGNFANATGSNSMAIQITGD
                     GLDKWIELTDEPNFIDLPLEEMKKRIGEAGICGMGGAGFPTLVKLSPPDDKPIDTVIL
                     NGVECEPYLTSDHRLMLEKTAEIVLGMKLIMKILGAQKGMIGIEANKPDAIAKMQEIT
                     KNEPAIEVVPLQLRYPQGAEKQLIYAATKRKVPAGGLPMAVGVVVQNVATAFAIYEAI
                     RYQKPLVQRVITVTGSPVVSPKNIMARIGTPLSELIEFCGGTSEEVGKVICGGPMMGF
                     ALPSLEATIGKGSGGLVLLNKKAASLNEEGNCLRCARCVDVCPMNLVPCMIVSAIKYN
                     NLDLAVKSGLNDCIKCGACAYVCPAQIRLVQYIDTGKIRYAETKQAKK"
BASE COUNT         2381 a         1493 c         1322 g         2225 t
ORIGIN      
        1 atgcctgccg gaatgcacag tgtgttgtgg gacggcagag ataataataa tgcagttgta
       61 gccagtggag tatatttcta ccgtcttagc agtataaata atgtccaaac taaaagaatg
      121 ctactaatga aatagaattt aaggatagga agcgggtttc cgcttctttt tcagttactt
      181 gtggttacag catactttaa gacacttccg tctcaatctt cccatcagaa ttgaatacta
      241 ataagaccat tcctgtgata actcttatgg tggtttacga atatatacag ctgataggca
      301 agaaaataag tggtgggaga tactttttac ctttaccacc catcagaaaa tcaaattttg
      361 cttgacatat ttttagaaag cacaaacaat gaaatatgta gtacataaaa attaataaag
      421 gagaaaacaa tgaaacaatt cttagtcgtt gtgatgcttt tcagctgtat tgggttagca
      481 gctattggtg aaggtgcttt tctactgctt cgttttgaac catcaaatat taatcggggg
      541 atgggcgaaa ttactggggt agttaatatt tggcataata atccttttac cgcatatagc
      601 aatccagcta tttcttcctt tcaaaagggc tttgcctata gttatgctca tgaagattgg
      661 atcgattttg ggtcggatag caaacttgaa tataatagag ctctgatgac tattgcctat
      721 aatggatttg ctataactct tcccggatat aatagtgatc aacgatgggg aatatctatg
      781 cctattacat atactttata tccttttact gatgtaaaca tagaagatta tacccaaatt
      841 cgtgcctttg gtttagcgat aaacccttgt gaaatattcc ggaataatca tcttgaaaca
      901 tatccttggc ttaagtatat tgatcttgcc ataggagcta actacatacc tctaaaagat
      961 gaagttacat atattatata cgatgaggat attgatgatt accaaatgga gaagatgaaa
     1021 gtggatgcgg atgtattaaa tattggagaa attctcagat tgaaatattg ttttcaggat
     1081 aaactggctc tggaagcagt atatggactt actcatttta atgcaaatgc cgaacatgta
     1141 aagtatgata atgatacaga ggcagaacct atttatgctg atcgaaatca aggttttgct
     1201 ttctctgcag ctgtcaaaac gaatccgata ttggttaata ccaaattgca taacttgggc
     1261 ttatttgata atctattcag ctttcgatat ttgaattcag ctttagtccc aaattataaa
     1321 aatagtgagg aaataactgg aaatggaatt gaactgggtt ttctggatac tttttattat
     1381 cgttctggta attatgatga cgatgcaggc ggaattagcg gatccacaaa tggttgggga
     1441 attaacttac actacaaaga tctgatttct tttggttaca actattctga gacccctggg
     1501 ggagatttgg tagataagca taagagcaaa gaatatagct gtagctttga ttttatcggt
     1561 atttatgata taatggtaaa ataaaaaata acactgaaaa aaatgatgtc cccgtcattc
     1621 ctttacaacc atactatgaa gtatcctctt caataatagg tagcttcata aaatgattga
     1681 taggtttgcc agggacatca accttccaga tattagcaag gattcctgat ataaagttct
     1741 gtcaggaacg atacatatta acgacgggtt tttaacccgg cgaaaatact ggagggttga
     1801 cctcctggtc aaccacaaca aacaaactat caagcttgta tagaataaag cgggaacttg
     1861 ataactcggc tgtcagggga acgacaccct accgttcttc ctttggatta tcctctcaca
     1921 gattacacag attgattttc acgcggatta cgcagattac gcagatttat attttataaa
     1981 ctaggagggt tgacctcctg gtcaaccctc aacaaaaann nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnga tttatatttt ataaactagg agggttgacc tcctggtcaa
     2161 ccctcaacaa aaacttctat cctataaatc ctaaaatccc agttctataa atcctaaaat
     2221 ccctgttcta aaaagttctt caatccttgt tccgaacatt taagttcggt tagctatttt
     2281 caaatctata atggctttag tagggcatac agttgcacaa gttccgcaat cggtacattt
     2341 gctataatca attcttgcta tattattttc aacggtaatt gcctgagcgg gacacttctt
     2401 ggcgcataaa ccacagccaa tgcaaggttt atctgttccg caattctgtt taggcagcgg
     2461 gtttttatct ttggaactgc aagtaatgaa cacattccta ctttctggaa tcagcattat
     2521 caatctacgc gggcaagcat taacgcaagc tccgcaaccg gtacattttg ctctatcaat
     2581 aattcgcaac ccattttcat caacactgat cgcatcaaat ttacaggcag ctacacaatc
     2641 attgaaacca acgcagcccc aggagcaaag atttggtcca tcggcaatat ttactgcaga
     2701 aaggcagctt tcaattcctt gatagtcata tttccattta gtattgtttt ttccaccgga
     2761 actgcaatgg atataggcaa tttttcgttg ttgggcactt gcttcttttc ccataatttt
     2821 agctattttc cctacgacag cggctcctcc gggagcgcat aaattcaatt ctttatcttc
     2881 agttacaata gcagtggcat atcctgcgca tcctggttga ccacaggcac cacaatttgc
     2941 accaggtaat acttcaatta tttgttcaat ccgttcatct atattaacag caaagacctt
     3001 ggaagcgaag gcaagaacaa gcccaaaaat caatcccaag gaacccataa tagcaacggg
     3061 aagtaatatt gtagaaattc caccggcagc taaaggtacc acgattatac ttaacatttc
     3121 ttcaccattc ccttaattaa atcttcatac ccataaatcc gtaaaacgct aaagccattg
     3181 ttccggcaag aatcaatccg cttggcattc cacgcatact ttttggcaat tctaccattt
     3241 ccaatcgttc tctgattcca gccattacca aaagcacaaa agtgaagcca ataccagaaa
     3301 ataatccgtt taacacagcc caaatgaaac tgtaacgaga gccatctgct agaggagtaa
     3361 tattaagtat ggcaacgccc aaaacggcac agttagtagt aatgagtggt agatatacac
     3421 ccaaggattt ataaagggct ggagaattct tctttataac catttccaca aattgcacca
     3481 aagctgcaat cgttaaaatg aaagccaggg tctgcaaata ttccagctgg aaaggaacca
     3541 gcagatattg atatattaga tagcagaagg cagaagccat ggtcatcaca aaaataacag
     3601 ccattcccat tccgaaagcg gaatccattt ttttggaaac acccaaatag gggcaaagac
     3661 ctaaaaaacg ggaaagcaca aagttctgga ttagaatggc agaaattgcc attgtgaaaa
     3721 gttgtccaag aaagcccatt attgtttctc cttcagcata ttcatcaatg ccatcaaaaa
     3781 tccgagagta ataaaagcac caggtgccag caatgtgatt aacattggat cgtaggtctt
     3841 gggcataact ctcatattta gccaagttcc gttacccaaa atctcacgga tagttgccac
     3901 aacagataag gaaagagtaa aacccagtcc catacctatg ccgtctgcaa tggaattgac
     3961 tacattgttt ttattggcaa atgcctctgc tctacccata attatgcaat taaccacaat
     4021 caagggaata aacaatccca aggatttatg cagtgcgggc aaatatgccg ccatactcat
     4081 atctacgata gatacaaaag ccgctatcac aattacataa acaggaattc gtatttggtc
     4141 tggggttata ttcttgatta gcgaaataaa gatattggaa caaattaaca caaatgttgc
     4201 cgccaaaccc attccaattg cattatttac agaagtagag gtggccaaag taggacacat
     4261 acctaagaca atcacgaagg tgggattttc ttttattata cctttggtaa gttctttaat
     4321 aaagctcatt tattcacctc ttcttttacc tttgtttctt cagacgaagt gctattttcc
     4381 tgaaccttag cttttagcaa ttcaatagat tcttttacag agtttacaac tgccctagtt
     4441 gttattgtag cgccagttat ggcttttata caattagggg gcacgcctcc atctttgtct
     4501 actattagtt gttcactggt tttaccttta aactgctctg agaatttggg ctgggtagaa
     4561 tttgatccca agcctggagt ttcagattga tcagtaactt ttatagcgat aatttggaag
     4621 ttattatcta ctgcaaccat agtttttacc ggaccgttat agccattttt ttcagcggta
     4681 aaaacatagc ccttcacttc tttagtttta ttatctttgg ctatgtagta agtaatatca
     4741 ctagtaattt cctcaaaatc ggaatctgga ataagtgttc tacgggtttc ttccgcttct
     4801 aatgtcttaa ttttgtcaat ttgaggttta gtaattgtat tcacataagc caaaagtgca
     4861 gttgctacta aacagaaggc aagtaagatt aagccaagtt gtaaatatat tttcattttt
     4921 tcacccatcc aaaagcttta ggcatagtta gtttatcgat tagaggggtt aacacattca
     4981 taaatagaat gctgtaggaa accccttccg gataaccacc atacaaacga ataaccatag
     5041 ttaatattcc gcaacctata gcaaatatta ttcttccatt tttagttata ggcgtagttg
     5101 tataatccgt tgccataaaa aaagcgccta agattaagcc accagaaaag atatggaaaa
     5161 atggcagcag aggactaaaa ccactacctt tcagcggagc caaaaagaaa gagagaacaa
     5221 acaccgttcc aatgtaatat aagggtatcc gccattcgat gatgtttttg taaagtaaat
     5281 aggcagcacc taaaagcaag gcaaaaacac tcacttcacc aatacaacca cctatatttc
     5341 cccagaagag atttttgatt gcagacaaat cagttaggtt agtaaaaatt cgattcgcta
     5401 aatctacacc ttggtctgta tttgtaatta tagaagaaac aaagctacta tctcttaagg
     5461 tttgagccac ttttaaagga gtggcacctg ttaccaaatc ataagctttg ggagataaac
     5521 tttgtaggtt cagggcaata gcatccagat ctgtgattcc acttaaagat ccacttttgg
     5581 gttttaccca tccagaagtc ataattgtag gccaggaagc aagtaaaaat gcgcggccta
     5641 aaagagctgg attaacagga ttattaccta aaccgccaaa aacctgtttt ccaactgcta
     5701 tagcaaaagc tgctcccaaa acaggaatcc accaaggggc tcctgcattt atattatagg
     5761 caagtagcat accagttaaa aaagcacttc catccgaaat cgtgattgga actttcctaa
     5821 atttctgaat tagcgcttca gttaccattg cagatacagc acctaaaaga gtaagcaata
     5881 gagatttaaa accccaataa taaacagcga aaacaagagc tggcgttaaa gccaacacta
     5941 cattccacat cacatttttg gttgtagttt tatcatgaaa gtggggagct ggagatacaa
     6001 tatatttatc gttcatttca ttctcctact ttttggcttg cttagtttca gcataacgaa
     6061 ttttgccagt atcaatatat tgaactaaac gaatctgagc agggcacaca taagcgcagg
     6121 caccacattt aatacagtca ttcaatccag atttcacagc taaatccaaa ttattatatt
     6181 ttatagctga tacaatcata cagggaacta agttcattgg acaaacatct acacagcgag
     6241 cacaacgcaa acaatttccc tcttcattta aactggctgc tttcttattt aatagaacta
     6301 atccaccact gccctttcca attgtagctt ccaaagaagg taaggcaaaa cccatcatcg
     6361 gacctccaca aataactttt ccaacttctt cgctagttcc tccgcagaat tcaatcaatt
     6421 cagacaaagg agttcctatt cgagccataa tatttttagg gcttaccacc ggagaaccag
     6481 ttacggttat aactctttgc accagaggtt tttggtaacg aatagcttcg tatatagcaa
     6541 aagcggttgc cacattttga actactactc ccacagccat tggcaatcca cctgcaggaa
     6601 cttttctttt tgtggcagca taaattagct gtttttcagc accttgtgga taacgaagct
     6661 gtaaaggaac tacttcaata gcaggttcat tttttgtgat ctcctgcatc ttagctatag
     6721 catcaggttt attagcctca ataccaatca tccctttttg ggcacctaag attttcatta
     6781 tcagcttcat cccaagtact atttcagctg ttttttctag catcaaacga tgatcactag
     6841 taagataagg ttcgcattcc actccattta agataacggt atctatgggt ttatcatcag
     6901 gaggagataa tttcactaag gttggaaaac cggcaccgcc cattccacaa attccagctt
     6961 ctccaatcct ttttttcatt tcctccagag gtaaatctat aaaatttggc tcatcagtaa
     7021 gctcgatcca cttatccaag ccatctccgg tgatctgtat agccatagaa ttcgaacctg
     7081 tagcattagc aaaattgccg atagccgtaa ctttcccaga aatggaagaa tgttgattta
     7141 gggatacaaa acctgttgat tctgcaattt tttgacccgt caaaacttca tctcctacag
     7201 aaacaattgg tttggaagga gaaccaatat gctgtgaaag atggattgtc actttttgag
     7261 gtataggaat atcaacaata ggcacagaag cactatactt tttctcatcc ggaggatgaa
     7321 cacctccggg aaatgtcttt aatcgcatca acactccctt tggtttgata gtatcaaacc
     7381 ggacatcaaa ttttaggtgc atttatatag agaggcatat ttaaggcaag taaaatttta
     7441 tgagagtgcc ctatgttgtt aaataggaag ccactatttt tgaataaatt ccgaatttgc
     7501 aggcagccta ttaatcttat g
//
LOCUS       DAOSTH010000073         7437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_9290_length_7437_cov_14.125982, whole genome shotgun sequence.
ACCESSION   DAOSTH010000073 DAOSTH010000000
VERSION     DAOSTH010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9290_length_7437_cov_14.125982"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..3022)
                     /locus_tag="PLB65_07060"
     CDS             complement(251..3022)
                     /locus_tag="PLB65_07060"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78324.1"
                     /translation="MKRRCLFFLMALSVAFVFAELGPVNEMGDSFFAGKLGCYAKGDT
                     LAYVSAKRSENNNLYYHILFQISYDGGQIWQSSVVDSSLFHPTEPTLYYSPEEIIVSW
                     NTREEHLCKYAQSINSGASWNISQNWNSFESSPYLEKYNGQLRQFSLKVPYPQWDQER
                     YLMPDEPDRFKPVHLYFKNTLSPNQTDTYFYGYEDITGVVRSNSDIKIKQLGGGQNNG
                     WPTFHEPVIISGSVNSTPSTYPIDAVFQGGLIENAPPLEFSDFIFTRSNYLPVGPTNY
                     DPNCILLVDVNGATYTSYLGHIIPPHTVTTDVWTNYPVGIEDPPLFTNTFAVSDTIWT
                     PGPSGVCSNRGFFVNSKLWIKGTFSGHQVWCAADTIMIIGEILLSGTVPGESPENNPN
                     DSVKLVSEKSVLLKYGYVDPVSGERIHPNCFSDDNPGNYYTDIYALREDPEHPYRKDG
                     IFSFEYQHPHPSMPDLVINDSLYTWVDLHRYHYPQTSVNPWSANLDLPWYNPLWPEAQ
                     PYLERGKIQLWGSVNQICRGFMHRSLSDSEYPSNSGIWNPENDYCGGISSVHYNDPAT
                     GILLSTQNFPGAAGSGVGYIRDYHADNRFSWGIEAPNPIFYPWKLGISLEDFVNNDLT
                     DIDWVPQYRPINSKCFTRKADKAYFASNDVLASRIGEIPMLEDLTSLTQNQGDICGLH
                     ILADNALLVHQEKEVNGSRELIIKVFSDVNHTLTDSFSLPVFSGMNDVAVLGNGTIIL
                     ATLGANRTINLRRLNSDGTFTTIDTWQLNFLDPQTQIHPKSKICLQPRHQNALEVTFM
                     LFAEGENGEPDYYQLYRADASVPLAIEDPSVPAIEPVMFYAYPNPVHTNLKLQVKVPN
                     QNTHKIEIYNLKGQKIATLKGNSAKNEIAEYNWNGCDAKGKSVSAGIYLVRLTVDGKV
                     VQSKRICKY"
     assembly_gap    3245..3344
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3405..4676)
                     /gene="eno"
                     /locus_tag="PLB65_07065"
     CDS             complement(3405..4676)
                     /gene="eno"
                     /locus_tag="PLB65_07065"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HOQ78325.1"
                     /translation="MSEIIYIKGREILDSRGNPTVEADVHLESGVVTKAGVPSGASTG
                     EREAIELRDGDKKRYLGKGVLKAVANIDLICDRLSGYDSLMQASIDKAMIDLDGTPNK
                     AKLGANAILAVSMAVARASAIELDIPLYRYLGGVNAKTLPVPMSNILNGGAHADNNVD
                     IQEFMVVPLGAETFRQALQMNAEVFHSLKAILKKRGMVTSVGDEGGFAPNLASNEEAF
                     IVIVEAIETAGYKPGKDIYIAIDAAASSFYQDGQYVFEGKKIDSEAMIEYYENMLTKY
                     PICSLEDGLAENDWSGWQKLNAKLGSKIQIVGDDVFVTNPAIIARGIKDNIANSVLIK
                     LNQIGTVTETIEAINMAHRAGWTTVVSHRSGETGDTFIADLAVALNSGQIKTGSISRS
                     ERIEKYNQLLRIEEELGETAYFPGKSIIKQL"
     gene            complement(4765..5013)
                     /locus_tag="PLB65_07070"
     CDS             complement(4765..5013)
                     /locus_tag="PLB65_07070"
                     /inference="COORDINATES: protein motif:HMM:NF013999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein"
                     /protein_id="HOQ78326.1"
                     /translation="MKEVPNFSKETIEQELKKIIYPQVGEDIIALKMVKYIGFKDGEV
                     IIDMNMMKFDQWRDNIADEIKEHLQKYPEVKKITINFP"
     gene            complement(5026..5388)
                     /locus_tag="PLB65_07075"
                     /pseudo
     CDS             complement(5026..5388)
                     /locus_tag="PLB65_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
     gene            complement(5390..6580)
                     /locus_tag="PLB65_07080"
     CDS             complement(5390..6580)
                     /locus_tag="PLB65_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HOQ78327.1"
                     /translation="MKIGRIYLDNCVTSKTAPEVLKEMQPYFEEKYWFPGTFISTGES
                     ANELISHSQQIVAESMGAKASEIHFTSGGTLANNIAIKGLANAYKKQGNHIIVSVVDY
                     PDLLTNAAYLEKNGFEVTYLSADNEGFISLTELQSAIRKETILFMTTAVNHTVGTIQP
                     LQEIKKVLESADHKIYFHLDAGQGYGKIPLNVNELGVDTMSISAHKIHGPQGIGALYL
                     RTGTKLTQLIHGVKRVDNLQTGGLSIALIAGFAKAVEITFSDFEKNTAYLRELSNYLL
                     EKMEQEIPYIELNGPRGEKRAPHNINVSIDFIEGEAITMMLDQKEITVATGSACASEG
                     LKANYVLMAMGKTHLQSHGSMKFTVSRYNTKAEIDETVQALKEITTELRSRSPLYQAL
                     LKEK"
     gene            complement(6580..6993)
                     /locus_tag="PLB65_07085"
     CDS             complement(6580..6993)
                     /locus_tag="PLB65_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOQ78328.1"
                     /translation="MAVNTRTEYALRALLEIADNPHEAVSAQKICQNQSLPKKYIERL
                     LTNLKTANLIVSSSGVRGGYFLSCNPEDISFLDILTAVQDESLDPTCNLDSRRYCPEG
                     ECPLSVFFKTLGDRIECILSEYSLADIYNAWKGEK"
BASE COUNT         2136 a         1497 c         1356 g         2348 t
ORIGIN      
        1 tgaagttatt gtaacataca attcggtgtt agtatttgtc gtcccgatgg ggctatttta
       61 tacagggaag gaatatttat tttttatctt attgtactcc gatgctggta atatgtgtat
      121 agataatgta ttatagtggt aattagttct ctgccctaca aaagatatct acagagaatg
      181 ggttatcaag gtgcaaatgg cttttcttaa gttggagggt tgaccagaaa agtcaaccct
      241 cctttataga tcaatattta cagattcgct tactttgtac aaccttacca tcaacagtta
      301 agcggacaag ataaattccg gcagaaacac ttttaccttt agcgtcacag ccattccagt
      361 tgtattcggc aatttcgttt ttagccgaat ttccctttag agtagcaatt ttttgtcctt
      421 tcaggttata aatctcgatt ttatgagtat tctgattggg aaccttcact tgaagtttca
      481 aattggtatg aacagggttc ggataggcat agaacataac aggttctatg gcaggaacag
      541 aaggatcttc gatggcaaga ggcacggatg catcagctcg gtaaagctga taataatccg
      601 gctctccgtt ctctccttcc gcaaatagca taaaggttac ttccagagcg ttttggtgac
      661 ggggttgcaa gcatattttg cttttaggat gaatttgggt ttgcggatcc aggaaattca
      721 attgccaagt atctatggta gtaaaagtcc catcgctatt taatctccga agattgatcg
      781 tccgatttgc tcccaaagtg gcaaggataa tagttccatt tcctaaaacg gctacatcgt
      841 tcattcctga aaaaactggc aaggaaaaag aatcggttaa agtatgatta acatcgctaa
      901 agaccttgat tatcagttcc ctgctgccgt ttacctcttt ttcttgatga actaacagag
      961 cattatccgc caatatgtgc aaaccacaaa tatcgccctg gttttgggta agcgaagtta
     1021 ggtcttcaag cattggtatt tcccctatcc tactggcaag aacatcgttg gaagcaaaat
     1081 atgccttatc cgctttgcgg gtaaaacatt tgctgttaat gggtctatat tgaggcaccc
     1141 aatctatgtc tgtaagatca ttgttaacaa aatcttcaag ggaaataccc aatttccagg
     1201 gataaaaaat cgggttcgga gcttcaattc cccaactgaa acgattatct gcatgataat
     1261 ctcttatata accaacccca cttcctgctg ctcccgggaa attttgcgtg cttaagagaa
     1321 ttccagtagc cggatcatta tagtgaacag aagaaattcc accgcaataa tcattttccg
     1381 gattccagat accactgttg gaaggatatt ctgaatcact caaagatcga tgcataaaac
     1441 cgcggcatat ttgatttaca gaaccccaaa gctggatttt ccctctttcc agataaggtt
     1501 gagcttcagg ccagagtgga ttataccaag gtaaatccaa atttgccgac cacggattta
     1561 ccgaggtttg aggataatga tagcgatgta gatcaaccca agtataaaga gaatcattaa
     1621 ttactaaatc aggcattgag ggatgaggat gttgatattc aaagctgaaa attccatcct
     1681 ttctatacgg atgctcagga tcttctcgca aagcataaat atcggtgtaa taattgcctg
     1741 gattatcatc gctaaaacaa ttgggatgta ttcgctcccc actaacagga tccacatagc
     1801 catatttaag tagaacactt ttttcggaaa caagcttaac ggaatcgttt ggattatttt
     1861 caggtgattc acctggaaca gttccgctta ataaaatctc accaataatc ataattgtat
     1921 cggcggcaca ccaaacttgg tgaccactaa aagtcccttt tatccaaagc ttggaattta
     1981 caaagaaacc ccgattactg caaacacctg aaggaccggg tgtccagatt gtatcggata
     2041 cagcaaatgt attggtaaat aaaggtggat cttcaattcc tacgggataa ttagtccaaa
     2101 catcggtggt tacagtatgt ggtggaataa tatggccaag ataggatgta taagttgcac
     2161 catttacatc tactaaaaga atacaattgg gatcataatt tgtaggacca acaggaagat
     2221 aattgctcct ggtaaaaatg aaatcggaaa attccaaagg aggagcattt tctattaacc
     2281 caccttgaaa tacagcgtct atgggatatg tggaaggagt actattaaca ctacctgaaa
     2341 taattactgg ctcgtggaaa gtaggccatc cattattttg tcctccacct aattgtttga
     2401 ttttaatatc tgaattacta cgcactacac ctgtaatatc ttcatatcca taaaaatatg
     2461 tatctgtctg atttggactt agtgtatttt tgaaatatag atgcactggc ttaaaccgat
     2521 ccggttcatc cggcattaaa tatcgttcct gatcccattg aggataaggg acttttaggg
     2581 aaaactgtcg taattgtcca ttgtactttt ctaaataagg actgctttca aaggaattcc
     2641 aattctgaga aatattccaa gaagcaccag aattgatact ttgggcatat ttgcagagat
     2701 gctcttcccg agtattccaa cttacgatta tttcttccgg actgtaatat aaagtcggtt
     2761 ctgtgggatg aaacaaagaa ctatctacaa cactgctttg ccaaatctgg cctccatcat
     2821 aactaatttg aaaaagaata tgataataca aattgttatt ttcagaccgc tttgcagaaa
     2881 cataagctaa agtatcacct ttagcataac aaccaagctt gccggcaaaa aaactatcac
     2941 ccatttcatt taccggaccc agctccgcga aaacaaaagc aacgctaagc gccatcaaaa
     3001 agaacaagca acgccgtttc atcgtatcct cctgatacaa aaaatattta cttaatatat
     3061 ttaagcacta ttcgttccaa aattagtata tatttatttg tcaattagtt aacttggatt
     3121 actattgctc atcttgaagc tttattgagt tttgggtagc cggcggacgc cgttccgccg
     3181 caaaagaaag ggtagccgga gctcgctgaa gctccgcttt ttttcttgcg gcggaacggc
     3241 gtccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntttttc ttgcggcgga
     3361 acggcgtccg ccggctacac attttatttg tgttgtattt atctttacaa ttgtttgatt
     3421 atggactttc cgggaaaata agcggtctca cccaattctt cttcaatccg taagagttgg
     3481 ttatattttt ctattctttc gctgcgagaa atagagccgg ttttgatttg tccagaatta
     3541 agagccacag ccaaatcggc aataaaagta tcacctgttt ctccgctgcg atgacttaca
     3601 accgttgtcc agccagctct atgagccata tttatagctt caatggtttc tgtgacggtt
     3661 ccaatttggt taagcttaat taacacgcta ttggcaatat tatcttttat cccgcgggca
     3721 attattgcag gattggtaac aaaaacatca tcgccaacaa tctgaatttt actacccagc
     3781 ttggcattca atttttgcca gccagaccag tcattttccg ctaagccatc ttccagagaa
     3841 cagataggat atttggtcag catattttca taatattcaa tcattgcctc gctatcgatt
     3901 tttttgcctt caaaaacata ttggccatct tgataaaaac tgctggcagc ggcgtcaata
     3961 gcaatataga tatcttttcc aggtttgtaa cctgcagttt caattgcttc cacaataact
     4021 atgaatgcct cttcattgga tgcaagattg ggagcaaaac caccttcgtc acccacggaa
     4081 gtaaccattc cgcgtttttt gaggattgct tttaaggagt gaaagacctc agcattcatt
     4141 tgcagtgcct gccggaaagt ttcagctcct aaagggacaa ccataaattc ctgtatatcc
     4201 acattgttat cggcatgggc tccaccattt aaaatattgc tcattggtac gggtagagtt
     4261 ttggcattaa cccctcccag ataacgataa agagggatat ccaattctat agctgaagct
     4321 ctggcaacag ccatagaaac tgctaagatg gcatttgcac caagtttggc tttgttggga
     4381 gtgccatcca gatcaatcat agctttatct atacttgctt gcattaggga atcatatccg
     4441 gataatctgt cacatataag atcaatatta gcaactgctt ttaacacacc tttgcccaag
     4501 tagcgttttt tatccccatc ccgaagttct atggcttcgc gttcacctgt ggaagctcca
     4561 ctggggacac cagctttagt taccactcca ctttccaaat gcacatccgc ttccactgtt
     4621 ggattgcctc ttgaatccaa aatttctctt cctttaatgt aaataatttc agacatttta
     4681 tcctcctgtc ttgtctaata taagtttata ataaattaac cgaaaatgcg aaaaaagcaa
     4741 atttggttaa tctacctatt gatattacgg gaagttaata gtaattttct tcacttccgg
     4801 atatttttgc agatgctctt ttatttcgtc tgcgatatta tcacgccatt gatcaaactt
     4861 catcatattc atatcaatta taacttctcc atctttaaaa ccgatgtatt tcaccatttt
     4921 caaagctata atatcctctc ctacctgagg ataaatgatt ttcttcaatt cttgttcaat
     4981 ggtttcttta ctgaaattgg gaacttcttt caagccgtga gcaatttcat aattggaaat
     5041 agcggattgt aatgtatcgg cagctaaaac actgcagtga actttaacgg gagggagacc
     5101 atctaaggct tccattgcat cacgccaggt tatttttttg gcttcttcca aagttttacc
     5161 ttttgccata tcggtgataa tggaagcggt ggcaatattg gaagcgcaac cataggacag
     5221 gaaactgata tcttcaatga ttttggtttc atcattaact tttagatata cggttacctg
     5281 atctccacaa gcagggcttc cttctgtagc tgtagcatca ggattttcca ttttgccaac
     5341 attgtgcgga tgcataaagt ggtctaaaac cttttgtgaa tattgcattt tatttctcct
     5401 taagtaatgc ctgataaaga ggactgcgac ttcgcaactc agttgttatt tcttttaaag
     5461 cctgaactgt ttcgtcaatt tccgctttag tattataacg ggacacagtg aatttcatcg
     5521 atccgtgtga ttgtaaatgt gttttaccca tcgccattaa aacataattt gcctttagtc
     5581 cttctgaggc acaagcactt ccggtggcaa ctgtaatttc cttttggtct agcatcattg
     5641 taattgcctc accttcgatg aaatctatag aaacattaat attatgcgga gcgcgttttt
     5701 cccctcgtgg tccatttaat tcaatatagg gaatttcctg ctccattttt tccagaaggt
     5761 agttggatag ttctcttaaa tatgcggtat ttttctcaaa atcgctgaaa gtaatttcca
     5821 cggctttggc aaagccagct attagagcaa tacttaaacc gcctgtttgt aaattatcca
     5881 ctcttttcac tccatgaatc aattgtgtca gttttgttcc agtgcgtaaa taaagggctc
     5941 cgataccttg aggaccgtgt attttatgag cactgataga cattgtatca actcccagtt
     6001 cattcacatt tagaggtatt ttgccatatc cttgtcccgc atccagatga aaataaatct
     6061 tatgatcagc actttccaag acctttttta tttcctgcaa gggctgaatt gttcccacag
     6121 tatgattcac agcggttgtc ataaataaaa ttgtctcttt gcgaatggcg ctttgcaatt
     6181 ctgttagtga aataaaacct tcgttatcag cagatagata tgtgacctca aaaccattct
     6241 tttccagata ggcagcgttg gttaacaaat caggataatc tactacagac acaattatgt
     6301 gatttccctg ttttttatag gcgtttgcca atcctttgat agctatattg ttagctaatg
     6361 ttccccccga agtgaaatga atttcactag ctttggctcc cattgattct gccacaattt
     6421 gttgagaatg ggaaatcagt tcattggcgc tttctccagt ggagataaaa gttccaggaa
     6481 accaatattt ttcttcaaaa tagggttgca tctcttttag cacttccgga gctgttttgg
     6541 aagttacaca attatccaga tatattcttc ctattttcat catttttctc ctttccaggc
     6601 attataaata tccgcaagag aatattcgct tagaatgcat tcaattctat ctcccaaggt
     6661 tttaaaaaat acgctaagag gacattctcc ctcaggacaa taacggcgtg aatctaaatt
     6721 gcaagttgga tctaaggatt catcctgaac cgctgttaaa atatctaaaa aagatatatc
     6781 ctcaggatta cagcttaaga aatatcctcc tcgcactcct gaactgctta ctatcaaatt
     6841 cgcagttttt agatttgtta agagccgttc tatatatttt ttgggtagag actgattttg
     6901 gcagattttt tgtgcgctaa ctgcctcgtg agggttatca gctatttcca gtaaagctct
     6961 tagcgcatat tctgtcctag tattaactgc catattttat ctccttaaaa tattctctct
     7021 aatccatact aatttgatat ggattcctgg caagtatttt ttaagccccg tagaatatgt
     7081 gaggtctaaa agatcagcgt aatattgaga aagccctaaa agattctctt ggtgagattc
     7141 agccatcatc aagaacaatg ttttactgta atgaaattgt tcaggatgat tctaacctat
     7201 tttattacta taaagagtga ttaggcacta tggaatataa ttagaaccga tggaaaaatt
     7261 ttaaccgcag aatatttcac caaacagtta taaaattgta tgtaccggcg gtcgctgaac
     7321 cgacgtgatt cagcttgaaa ttgtatgtac cggtggtcgc tgaactgccg tgtttttgga
     7381 accaaacagt tatagagtga aattgtcagt accggcggtc gctgaaccgc cgtgttt
//
LOCUS       DAOSTH010000074         7437 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_9291_length_7437_cov_13.175427, whole genome shotgun sequence.
ACCESSION   DAOSTH010000074 DAOSTH010000000
VERSION     DAOSTH010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7437
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9291_length_7437_cov_13.175427"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(359..1096)
                     /locus_tag="PLB65_07090"
     CDS             complement(359..1096)
                     /locus_tag="PLB65_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HOQ78329.1"
                     /translation="MNDNNSDMLFSDVYNNVLPFRDLILRISKIAKVIHQQGWAEANA
                     GNISIRISNMLIPFIQKNGWENNYPYQDWFLVSKTGSRFRDMIDEPENCLMIIGVGNE
                     EHYFPEDAKPTSEWLCHRKMQEMNKNTNLSCILHTHPTEVIALSNTSFFIDNKKYPKC
                     LNEHFYEALPEIRFFLPQGIAIADYAPPGSSVLAKKSCKSINDKQALIWEKHGLLVIA
                     ENIDIALDLTEVINKAAKLCLLKLLKS"
     gene            complement(1257..1895)
                     /locus_tag="PLB65_07095"
     CDS             complement(1257..1895)
                     /locus_tag="PLB65_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78330.1"
                     /translation="MSGFMATFGLWLSVFFTLSIFSFLYKDNPFYKLSEQIFVGLSAA
                     YWLVYLYYSVMVPNLFNKLFTDFKGNLILLIPTVLGLMMLLRLIPKTQWLSRYPIAIM
                     IGTSAGINMVRYAKTDLLGQISATMINPFAADNVVTVIGNILLIVGTICGVYFFYFSK
                     KQEGIRAVPSKLGIWFLMISFGASFGYTVMARISLLIGRLEFLLKDWLHIIK"
     gene            complement(1892..2722)
                     /locus_tag="PLB65_07100"
     CDS             complement(1892..2722)
                     /locus_tag="PLB65_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78331.1"
                     /translation="MSANTNSQTERRIIYLVLLIAVALPLIFPIGWKTEISGYTKLAW
                     ELIQNTPDSSVVVFSFDYDPSTLTEIQPMAEAMLEHSFSRNQKIIAMALWPQGVQMAD
                     QAFTEALKKYPDKKYGVDYVNLGYKVAGIVAIQAMGKSMPEVFPSDQRGIKYEDIPML
                     KKVKTLRDVAFICSLSSGTPGIKEWVMAARDPLGTPVTGGTTAVSAPGLLPYVNSQKQ
                     LYGLLGGLKAASEYETLINKIGSATIKMDAQSIAHLLILVFIIIGNIKAFYNSKRSEQ
                     "
     gene            complement(2719..3417)
                     /locus_tag="PLB65_07105"
     CDS             complement(2719..3417)
                     /locus_tag="PLB65_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78332.1"
                     /translation="MKYKVPLIFAFVCGILVVISEFIPHRPFNMLITTLQDWFMIISG
                     FAIILGQISLFQANIKKIRGKNPDWPFYLAGLISFSIMLIVGLFWGTQEYRGILGHGD
                     KIQAAIGMKPFDYLFANAFMPLSSTMFSLLAFFIASAAYRAFIIRSFESNLLMITAVI
                     VMLGRTSFGGMLTSWLPQGLYFLHLPNLSDFIMEFPNSAAQRAILISAALGIIGSSLR
                     IILGIERSYLGGDK"
     gene            complement(3414..4514)
                     /locus_tag="PLB65_07110"
     CDS             complement(3414..4514)
                     /locus_tag="PLB65_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78333.1"
                     /translation="MKKILLVLLMGLLLSFLFAKSSFFWVEDVPWDDGKALIIKANVP
                     ADTVYLQKALEDGSWQTLYAGKGINLEYTDSELNPDLEYRYRLLVPRPTYGDSISFKA
                     IAKPIEQWFYLHKLSYLLILLAVSFAIVYYVQKARKGKDYYIRRINGLDSIDESIGRA
                     TEKGKPILFVPGIGDLDDIQTISSLTILSHLAHKAAEYDTEIIVPCRFSMVLSASREV
                     VKEAYIKAGKPDAYNQDRIFYLTDDQFGYVAGIDGIMVREQPAANFFLGSFYAESLIL
                     AETGFSTGAIQTAGTAQASQLPFFVVSCDYTLIGEELFAASAYLSRDPQQLGSLKGHD
                     FGKLLVILLIVAGVIAEMSNWHWFKAFFEGAQ"
     gene            complement(4586..5383)
                     /locus_tag="PLB65_07115"
     CDS             complement(4586..5383)
                     /locus_tag="PLB65_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78334.1"
                     /translation="MRKQVWLIIPLIILIVIGGCKKKAHTLPDKETANAITAYAPIPS
                     YKEVFRFLDKYQAKEISAAIPDELYKTKQEDVRNAFSLGVISVDAILSAKGQNKKRLN
                     QYYSQMLDLTSVLGLDEEVNQISPNLKKHINEGQWLELETAIDAHKKKVEDIFWEKEI
                     FDYYTLLQLGSWIEATNRTAWLIQQNYSVEHTRALHQKEAFQNIIANMNNIDSEAIVN
                     QPEFQEVMGLVQQLKAIIDADYEKTYTSEQLDQIISLTNQIKETFQK"
     gene            complement(6690..7199)
                     /locus_tag="PLB65_07120"
     CDS             complement(6690..7199)
                     /locus_tag="PLB65_07120"
                     /inference="COORDINATES: protein motif:HMM:NF018154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HOQ78335.1"
                     /translation="MDSENEGINEPLLILIGGAPGTGKSSVASQLYCRLKNSVWLDGD
                     DLWRMNPFLVTEARKEMILNNAAFVLSSFIRESFSYIIFSWVMHEPAIVQRLLEKISI
                     RQYRLLHFNLLCSESTLMQHISTTDRDPGLCLERLRSIQENYPDAVDIDKMSVEDVVQ
                     CILKYIQAS"
BASE COUNT         2353 a         1449 c         1305 g         2330 t
ORIGIN      
        1 atccctttct attatagagc ggataaaatt ggaagttact gccttttctt ctttgttttc
       61 aattgtgttt tctttagtat ccatattcaa tccttttcct tatatacata taatcatttt
      121 ctgatagctt gtattcttgt caagtgcaat gaagtttatc tctcacagat taaattagaa
      181 cacggattta atggattata gcggttgaaa aaggtctttt attttttaag tacccatata
      241 aaaaccggtt tatccaaatg atccagttgt ttatcattgt tataagtcct tatcgctaaa
      301 tgtggttata gacaaggtta aagttataaa aatgatgatg aattttagct tacagacttc
      361 aggacttcaa gagctttaag agacatagtt tagccgcttt attaataact tccgtaaggt
      421 caagggcaat atctatgttc tcagctataa ccaagagacc atgtttttcc cagataagtg
      481 cctgtttatc gtttatactc ttacagcttt ttttagctaa aacagaactt ccgggaggag
      541 cataatcagc tatagcaatt ccttggggaa ggaaaaatcg tatttctgga agtgcctcat
      601 aaaagtgttc attcaggcat ttgggatatt tcttattatc tataaaaaaa gaggtattag
      661 aaagagcaat aacttcggtg ggatgggtat gaagaataca agacaaatta gtatttttgt
      721 tcatttcttg cattttacgg tgacagagcc attcggaagt aggttttgca tcttccggaa
      781 agtaatgttc ttcattacca acaccgatta tcattaaaca gttctccggt tcatcaatca
      841 tatcccgaaa acggcttccg gttttggaaa caagaaacca gtcctgataa ggatagttat
      901 tttcccaccc gtttttctgg ataaagggta ttagcatatt ggatatacgg atagagatat
      961 ttccggcatt agcttcagcc catccttgtt gatggataac tttagcgatc ttactaatac
     1021 gcagaatcag gtctctaaaa ggtaaaacat tattatagac atcgctgaag agcatatccg
     1081 aattattatc gttcattaac tcacctgttt cttttgtcat aatatatcaa aaacttcttg
     1141 atccgttcat aaagccaagg caggaaatga caagatgtca tttattccga tatccaaatt
     1201 ccatcttgcc aactcaccat ctcactatct cgcaacttca ccatctcact atctcactat
     1261 ttaattatat gcaaccaatc cttcagtaaa aattccaacc ggcctattaa taaagagata
     1321 cgagccataa ctgtataacc aaaagaagca ccaaagctaa tcattaaaaa ccaaattcct
     1381 agcttggagg gaactgctct tatgccttct tgttttttac tgaaataaaa gaaatatact
     1441 ccacaaatag ttcccacgat taggagtatg tttccaatta ctgttacaac attatcagca
     1501 gcaaaaggat tgatcattgt agcgctaatt tgtcccaata gatcagtttt agcatagcgc
     1561 accatattga ttccagcaga agtaccaatc attattgcaa taggatatct gctaagccat
     1621 tgtgtcttag ggattaagcg cagtagcatc atcaaaccca aaacggtagg aataagcaaa
     1681 atcaggtttc ccttgaaatc ggtaaaaagt ttattaaaca gattgggaac cataacactg
     1741 taatataaat agactagcca gtaagcagcg gaaagtccaa caaaaatttg ttcggagagt
     1801 ttatagaaag gattatcttt atagaggaag ctgaagatgc ttaaggtgaa aaaaactgaa
     1861 agccagagtc caaaagtagc cataaatccg ctcattgttc actcctttta ctgttataga
     1921 aagcttttat attaccgatg atgataaaca ccagaattaa aagatgcgct atagattgag
     1981 catccatttt aatagtagca ctgccaattt tattgatcaa agtttcatat tctgaggcag
     2041 ctttaagccc acccagtaga ccataaagtt gtttttggct atttacataa ggtaataagc
     2101 caggggcact gacagctgta gttccccctg taacaggagt tcctaaagga tcccgagcag
     2161 ccataaccca ttccttaata ccaggagttc cactggaaag agaacaaata aaggcaacat
     2221 ctcttaaggt cttaaccttt ttcagcattg ggatatcttc atattttatt ccgcgttgat
     2281 cagaaggaaa aacttctggc attgattttc ccattgcttg gatagcaaca atgccagcaa
     2341 ccttatagcc cagattaaca taatccactc catacttttt atcgggatat tttttcagcg
     2401 cttctgtaaa tgcctgatct gccatttgca caccttgagg ccagagagcc atagctataa
     2461 ttttttgatt tcgggaaaat gaatgttcca gcatcgcttc tgccattggt tgaatttctg
     2521 ttagggtaga aggatcataa tcaaaggaga aaaccacaac tgaagaatct ggggtatttt
     2581 gaatcagttc ccatgctaat tttgtgtaac cggagatctc ggttttccag ccaataggaa
     2641 aaatcaaagg tagggcaaca gcaattaaca aaactaagta gattattctg cgctcggttt
     2701 gtgaatttgt gtttgcgctc atttatcgcc tccaaggtaa gatctttcaa tacccagaat
     2761 aatgcgtaag ctacttccaa taatccctaa agcggcactt atcaaaatag ctctctgagc
     2821 cgcggaattg ggaaattcca ttatgaaatc actcaaattg ggtaaatgta aaaaatagag
     2881 cccttgtgga agccaagaag taagcatccc accaaaactt gttcttccta acattactat
     2941 tacagctgta atcattaata gattagactc aaaactacga ataataaaag cacgataggc
     3001 agcagaagca ataaaaaaag ctaaaagaga aaacattgtt gaggaaagag gcataaaggc
     3061 attggcaaat aggtaatcaa agggtttcat tccaatcgct gcctggattt tatctccatg
     3121 acccagaatt cctctatact cttgagttcc ccaaaaaagc cctactatta acataattga
     3181 aaaactaatg agcccagcaa gataaaatgg ccaatctggg tttttccctc tgattttctt
     3241 aatgtttgct tgaaacaaac ttatttgtcc caagataatt gcaaaacctg aaataatcat
     3301 aaaccaatct tgtaaagttg tgatcagcat attgaaagga cggtgaggga taaattctga
     3361 aataactacc agaattccac aaacgaaggc aaagataaga gggactttat acttcattgt
     3421 gccccctcaa aaaatgcttt gaaccaatgc caattagaca tttctgctat aactccagcc
     3481 actataagaa ggataactaa cagttttcca aaatcgtggc cttttagtga acccagttgt
     3541 tgaggatctc ttgataaata agcagatgca gcaaaaagtt cttctccaat taaggtgtaa
     3601 tcacaagaga ccacaaaaaa aggtaattga ctggcctgtg cggttccagc agtctgaatt
     3661 gcaccagtac taaatccggt ttccgctaag attaaagatt cagcataaaa acttcccaga
     3721 aaaaaattag ctgcaggttg ttcacgtacc ataattccat caattcctgc cacataacca
     3781 aattgatcat cagttaagta aaaaattcta tcctgattgt aagcatcagg tttcccagct
     3841 tttatatatg cttcttttac tacttctcga gaggcagata aaaccattga aaacctacag
     3901 ggaacaataa tctcggtatc atattctgcg gctttatggg ctaaatggct taagatagtt
     3961 aaactggaaa tagtttgaat atcatctaaa tcgccaatac ctggaacgaa aagaataggt
     4021 ttaccctttt ctgtggctct gccaatagat tcatcgatac tatctaagcc gttgatccgg
     4081 cgaatataat aatcctttcc tttcctggct ttttgcacat aataaacaat agcgaaagaa
     4141 acagccagca aaattaatag ataggagagt ttatgcaaat agaaccattg ctcaataggt
     4201 ttcgctattg ccttaaaaga aatactatca ccataagtag gacggggaac tagtaaccgg
     4261 tatctatatt ctaaatcggg attaagctca ctatctgtat actcaaggtt tatacctttg
     4321 ccagcatata atgtttgcca gcttccatcc tcaagagctt tttgcagata aacagtatca
     4381 gcaggaacat ttgccttgat tatcagtgcc ttgccatcat cccagggaac atcttcaacc
     4441 cagaaaaaac tggatttggc aaatagaaag ctcagcaaca accccataag taaaaccaat
     4501 aaaatctttt tcataatgct cctttagttc ctaaaatacg gtttaaatat ctgttctgaa
     4561 ctatgaaaca gcattgtcat gatcttcact tttgaaatgt ctctttaatc tgattagtta
     4621 gagaaataat ctgatccagc tgttcagaag tgtaggtctt ttcataatcg gcatcgataa
     4681 ttgcttttag ctgttgtact aaacccataa cttcttgaaa ttcaggttga ttaacaatag
     4741 cttctgagtc gatattattc atattggcaa ttatattttg aaaagcttct ttttggtgta
     4801 aagccctagt atgctccaca gagtaatttt gttgtattag ccaagctgta cgattcgttg
     4861 cttcgatcca gcttccgagt tgcagtaaag tgtagtaatc aaagatctct ttttcccaga
     4921 atatatcttc aaccttcttt ttatgagcat ctattgcagt ttccaattct aaccactgtc
     4981 cttcgttaat atgttttttc aaattaggac taatttgatt tacttcctca tctaaaccaa
     5041 gaacagatgt taaatccaac atctgagaat agtattgatt taatcgtttt ttattttgac
     5101 ctttagcaga tagtattgca tccacagaaa ttactcccaa agaaaatgca ttgcgaacat
     5161 cttcctgttt tgttttatac agttcgtcag gaatagctgc agatatttct ttcgcttgat
     5221 atttatctaa gaaacgaaaa acttctttat aagaaggtat gggagcatac gctgttattg
     5281 catttgcggt ttctttgtca ggaagagtat gagctttctt tttacaaccg cctatcacaa
     5341 tcagaataat gaggggaata attaaccaga cttgttttcg cattgtttac tccttatctc
     5401 taaatcatca ttttccaaaa agttatgtat acagcttagt gagttattca acttttttta
     5461 attaattttt gaccagagag aactgtcaag tacaaaactg cttttcttct ttccagctgt
     5521 ttcctaattg ttagagaaaa ttctaaccct ttgtttctct atctttttga cagtgttaga
     5581 tattccttgt taaacaatct tggtaagtcc aaacgaaatt agcattattt tcattctgcc
     5641 aatctcagta aaatctcctt aatagaagag atagttaatt atttcttttc agggagctcg
     5701 ctatcattgt gctgcttccc tttcacaacc gaaaataact ccattattcc tgattatgga
     5761 tgtgcaaaaa tatttcttca aaaaaagatc gcataataaa tttgcggata tgaactgtcc
     5821 tgcaacttta taatttttcc gagacaccct ccatatcggt atcaatctga taaactttct
     5881 caatcctcta tatcgttgta tttgtctttt cttagggaaa actgttatca aatctcgtta
     5941 tattgatatt gatggccagg aaatttcgtt aataatccgc cttccgcagc ccttttattg
     6001 aacagcaggt taattgtaat tataaactga catctataat tgtgagtagt gacaaagggg
     6061 agagtgagga taaggctttg attagcagga aggctctgga tgtccggcaa aacgaataat
     6121 tgtactacaa ctccatctat acctttttct cagtgtaatg ctcatccatt tactcctatg
     6181 taccagtact gaggatttgt atactcaaca tccatagagg taggatcata tggctgttgt
     6241 actagggcaa ttgcaagcgg taatctatat ggtgctaggc agacttttca aagtagctat
     6301 agccttgtat agatctacct ttgcctagat ctacctgtag gaataactca gtttcataat
     6361 ctcaaacatg gcacatttga tccctcaatt aagcttgaac ttccacactc aggtgaagga
     6421 actgaaattg cccgatatta ggatctccag gttgcttatg aaggaaaagc tggaaatcta
     6481 caagatcatt tcgatcgtca tagtgccgaa atcatcatcc aaagcagttc ataagccttt
     6541 gctctggatc tgagaggctt tcgaagtatg gggaaacgca attcgctgaa tactcttagt
     6601 tttacttacc gcgatatttt ttgtccttct ggggataggc ttgctgattg tgctagggtt
     6661 ttttcaaatg atctttctcg tcagttttgc tagcttgcct ggatatactt cagaatgcat
     6721 tgcacaacat cttccacact cattttgtct atatctacag catcgggata gttttcttgg
     6781 atggatctca agcgctccag acacaatccg ggatcacggt ctgtggtaga aatatgctgc
     6841 atcaatgttg actcactgca caataggtta aaatgcagca acctgtactg ccggatggat
     6901 atcttctcga gcaaccgctg gacgatggca ggttcatgca tcacccagga aaagataatg
     6961 taagaaaaag actctctgat aaatgaggac aacacgaagg ccgcgttgtt tagtatcatt
     7021 tccttacgag cctcagttac caggaaagga ttcatccgcc atagatcatc accatcaagc
     7081 catacgctgt ttttcaatct acagtaaagc tgggaggcca ctgaactctt acctgttcct
     7141 ggagctccac caatcaagat cagtaagggt tcattgatgc cttcattttc actatccata
     7201 atgctaccta atctgatttc agaagtgttt gacataatac ctcaatatga aaaaaaggat
     7261 ggttgagtat gtttgcgaag gagcaggaaa actgtcaaat atcttttagc tcttttagag
     7321 tagcgggcaa gttctatgag ttgtaaagtt atagatttca aaaaaaccta ggttgctgaa
     7381 gaactgtatc cagaatggag tttcctcgca ggatcggaat tctgcattac gacagtg
//
LOCUS       DAOSTH010000075         7407 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_9333_length_7407_cov_13.810256, whole genome shotgun sequence.
ACCESSION   DAOSTH010000075 DAOSTH010000000
VERSION     DAOSTH010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7407
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9333_length_7407_cov_13.810256"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(607..1317)
                     /locus_tag="PLB65_07125"
     CDS             complement(607..1317)
                     /locus_tag="PLB65_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HOQ78336.1"
                     /translation="MKKEVIICMAIQSLFLLLTLSACIPVNCDDNCEKGVRVNGVTLK
                     YKNSASLSQSYEDNNLEIKGGTLSIDLKGHSEPQADLQVEYCEYEPGDATIILKKGKI
                     SWESKSNKPVSLTRISGSIPEALNLNIETGTGETKLDAIKGNQSISVDKGTGRTEISN
                     SQFNQLTINSGTGYVTLINTSISKCSIDTGTGSVNLKNSVIEDAMIETGTGDIIIEKS
                     KINKQNFTTGTGKVITHN"
     gene            complement(1399..2448)
                     /locus_tag="PLB65_07130"
     CDS             complement(1399..2448)
                     /locus_tag="PLB65_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78337.1"
                     /translation="MLDIYADIKNISYQPTLCKPLPKYSVDKLSIALLKSCFQLEFSP
                     SVSFAISKWVSPKRTRSYPYTRVYNTYSFEGKRVTLIPVIKDEGLDGDRDFIQWDTIS
                     LMSLLQTYIIFVYYIEAEINPNYNNKITSQQISEEYLKIKLQELSVYKSDALHWNLNQ
                     TKNIAEILEKALQSYELMSSNLGVIFHSNKNAYSKIERIKADYNNFLIDSRINAKKAQ
                     YRESMTIQPKESIAIGQKAKITIENYLKGKYYLTVDEAIINGNQIQLIEAKHTNSDRL
                     PSYDDVIDGIVKMILFTNLDLVEIKGKRYRPQAVLKLTNKNGIKENNLSSSDNEFLMK
                     LRNEAKINKFKIVLG"
     gene            complement(2450..3277)
                     /locus_tag="PLB65_07135"
     CDS             complement(2450..3277)
                     /locus_tag="PLB65_07135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOQ78338.1"
                     /translation="MKTTHKLIIGSSELMPELADSSIQLIVTSPPYFNAPFDYEGFYY
                     NYDKYLEVMSTVSKEMYRVLDKGRILILNIDDMLVNGVKYPITADFTKILQGVGFLYR
                     DRIIWKKPDGYLRISRRSGVLLQHPYPMYFYPDNLLESLLIFQKGEFDYKSIPDTLKQ
                     ESKIDLDEFKKNKWYSNLWHITNVLPNAKYENGVAAFPDEIPRRFIKLFSYKGETVLD
                     PFLGSGTTMKVAIELQRNSVGYELKPELEEKITKKLKLDCTDIFIDYDVNFEVIRRF"
     gene            3632..4600
                     /locus_tag="PLB65_07140"
                     /pseudo
     CDS             3632..4600
                     /locus_tag="PLB65_07140"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            4888..7194
                     /locus_tag="PLB65_07145"
     CDS             4888..7194
                     /locus_tag="PLB65_07145"
                     /inference="COORDINATES: protein motif:HMM:NF024565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ78339.1"
                     /translation="MINKINYINNIGLFVNMRGASNIDLKRLTLIYAENGRGKTTLSA
                     ILRSLMTGDPIYINERRSIGSQSNPHVVLTCGGNQTVQFQNGSWNKSIQGIVIYDDVF
                     VANNVCSGLMVDASHRQNLHEFIIGEQGVRLNRQLYDYIEKIEAHNIELQRLSSLIPR
                     EARFGLNIDDFCSLQIEENIDNKIMALERDLLAAQNSVEISKADLFTPIHIPEFSQSA
                     YSDLLGSSLDTLDSTAMKLVEDHFNKYSHNPEQWISEGLDLLQSIPEETDDYICPFCS
                     QPLKGSIIIQHYRVYFSAEYKQLIGRINESIMFLNNQCGEDKLIAFERAIRSYNENRQ
                     YWSKFTDIPDIIINSDSVIQSWKNLRDMIYKKLSTKKSSPLDKVIFTEDELVLFSKYE
                     ASRKNIDKHNRQVAQINEELKTIQQRTKCADISVINSNINRLKAIKSRYSPDFSQYCE
                     NYLKEIANKQSTEILRDTARKELQDYRESIFPKFQEAVNHYLQVFNAGFTLCNLSPTN
                     IRIGSTCNYRVLINNIPVPITSEPGSQAFHNTLSSGDRNSLALAFLFATLSHDPNLSN
                     KIIIIDDPVSTLDEHRTLSTAQEISRLSSQAEQVIVLSHSKSFLCSVWDGTDRINCAS
                     LKIVREGTNSTINDWDVNSDSLTEHDRLFSLLDSYMNVENVNKKEVAIAIRPYLEGLL
                     RVNYPKYYKPGMMLGNFIHLCKQNMNTQEQILNEKQINELENIKEYADRFHHDTNPNW
                     QTLSVNDGELRGFVRRALNFTTISNSVT"
BASE COUNT         2468 a         1247 c         1297 g         2395 t
ORIGIN      
        1 taaataactt ttacacatcc cacaaactca tttggcagtg aggactaatg acgacgactt
       61 atttcacgca tatttcgctg attacgcaac tcactgtatt agtaggagtt atatataatc
      121 agtgtatatc tgaacaagcg gttaaatctg tgttactatt ctttatatta tatgagtaat
      181 ataagctgat gaagtttctt ataaggaaaa tcaatggaaa agagaaaaaa cgattaaggt
      241 ttttttaaga taaaaatggg gttgcaaaat gcctgattgc tcaagtaagg tagttccatt
      301 ctgcgtttgg tttatttcgc taaaccgata ctttatttga ttacaaagag gtgggtagag
      361 ctactgaata tctattactt tgtccttttc ttgcaaggca taagttcaga caaaagttaa
      421 aactaaatgg caactctata gatatgcatt cttccgaaat ttttaccaaa accagcaaaa
      481 caaagaaagt tatttagctc ttcctatggt cttcctttgg cttttccgat ttttactaac
      541 tttgagacac aaccctaatt gtagtgtacc taccctttat agagaaaaaa taagatagag
      601 ataaactcag ttgtgggtta tcacttttcc cgttcctgtt gtgaagtttt gcttgtttat
      661 cttactcttt tcaataatta tatctcctgt tcctgtttct atcatagcat cttctatcac
      721 gctatttttt aggttaacag agccggtgcc tgtatctatg gaacatttac tgatactggt
      781 attaataaga gtgacataac cggttccgct atttatagta agctgattaa attggctgtt
      841 agatatttca gttctacctg ttcctttatc tactgagatg gattgatttc cttttattgc
      901 atctaatttt gtttcacctg tacctgtttc tatgttcaag tttagagctt cgggaatgct
      961 tccactgatt cttgttaaag aaacaggttt atttgattta ctttcccagg atattttccc
     1021 ctttttcaaa ataatagtgg catccccggg ttcatattca caatattcta cttgcaagtc
     1081 agcttgtggt tctgagtgtc ctttcaggtc aatagataaa gttccgcctt ttatttccag
     1141 attgttatcc tcataactct gggataggga tgctgaattt ttgtatttta aggtaactcc
     1201 atttactctt acaccttttt cacaattgtc atcgcaatta acaggaatac aagcggaaag
     1261 cgttagtagc aaaaatagag attgtattgc catacaaata attacttctt ttttcatcag
     1321 ttctccttga attaaagctt tttatagcat attaaggtca tttcatcacc tatatccatt
     1381 attatcaaag caacggtttt atcctaaaac aattttgaat ttattaattt ttgcttcatt
     1441 gcgtaacttc attaaaaact cattatccga agaagataga ttattctctt taattccgtt
     1501 tttattggtt aatttcaaca ctgcttgtgg tctgtacctt ttccctttta tttcaactaa
     1561 atccagatta gtaaacaata tcatttttac aataccatct ataacatcgt catagcttgg
     1621 taatctatca gaatttgtgt gttttgcctc aattaattga atctgattcc cgttaattat
     1681 tgcctcatca actgttaaat aatattttcc cttgagataa ttttctatag ttatttttgc
     1741 tttctgacca atagcaattg attcttttgg ttgaatggtc atactctctc tatactgagc
     1801 tttttttgca ttgattctgg aatctataag aaaattattg taatctgctt taattctttc
     1861 tattttggaa taagcattct tattgctatg aaatatgacc cctaaattag atgacattaa
     1921 ttcataactc tgtaatgctt tttccaaaat ctctgctatg ttctttgttt gatttagatt
     1981 ccaatgaaga gcatcacttt tatatactga tagttcttgt agtttaattt ttaaatactc
     2041 ttcggaaatt tgttgggagg taattttatt attataatta ggatttattt ctgcttcaat
     2101 ataataaaca aaaataatat atgtttgaag taagctcatc aaagatatag tatcccattg
     2161 aataaaatcg cggtcaccat ccagtccttc gtctttaatc actggtatca atgttactct
     2221 tttgccttca aaggaatatg tattatacac acgggtatag ggatagcttc ttgttctttt
     2281 tggggagacc catttactga ttgcaaaact gactgaagga gaaaattcaa gctgaaaaca
     2341 ggattttaaa agagcaatac tcaatttatc aacagaatac tttggtaatg gtttacaaag
     2401 tgttggttga taagatatat ttttaatatc agcataaata tctaacatcc taaaatctcc
     2461 ttataacctc aaaattcaca tcataatcaa taaatatatc tgtgcagtcc aactttaatt
     2521 tctttgtaat tttctcttct aattccggtt ttaattcata cccgacacta tttcgctgca
     2581 gctctattgc cactttcata gttgttccgc tacctaaaaa agggtctaaa accgtctctc
     2641 ctttataaga aaacaactta ataaatcttc tgggaatctc atcaggaaaa gcagcaactc
     2701 cattttcata tttagcattt ggcagaacat tagtgatatg ccataagttt gaataccact
     2761 tattcttttt gaattcatct aaatctattt tggattcctg ttttaatgta tccggaattg
     2821 acttataatc aaattcacct ttctggaata tcaataaact ttctaataag ttgtcaggat
     2881 aaaaatacat cggatagggg tgttgtaata atacgccact tcttctgcta atgcgtaaat
     2941 atccatccgg ttttttccag attattctat ctctatagag aaaaccaacc ccttgaagaa
     3001 tttttgtaaa atcggccgtt ataggatact taactccgtt tactaacata tcatctatgt
     3061 ttaatattaa tattctgcct ttatccaaaa ctctatacat ctctttggaa actgtagaca
     3121 ttacttccaa atacttatca tagttgtaat agaaaccttc ataatcaaaa ggtgcattaa
     3181 aatagggagg agatgtaact atcagctgaa tagagctatc tgccagttca ggcattagct
     3241 cgctgcttcc gattattagt ttatgtgttg ttttcattat aatccttaat aaaaacacca
     3301 gtaataaatt caatttaact gtcaaggttt ttgaattttc agaagtagtg tctcaaaggt
     3361 tatggtaaaa tcgggtaagc cagataaata acaaggttga gccagaacct actctttgtt
     3421 atgatggttt aattaaaata aaagaataag aagaagatta tgactcaacc aaaaagatag
     3481 aaagagaaaa gagtagcatc agtcatgcta attcagtgta attcaaaatg gcttatttaa
     3541 aatgtagaaa aaaaacatac aaagagttgt agaagaagaa gttagtatga tacttagtcc
     3601 agaatagtat taagatactg caaagaggac taactatcgt aatggttaca gagagcgcaa
     3661 ggagccactc gttacaggta taggtccagt gaacataagc atacctaagt taagaaaagg
     3721 aagtttctat ccgacaattt tggagcaata tcagcgggta gatagagcta ttatcagcat
     3781 tatcagtgat gcttattttg ccggagtttc tacccgcaag atgaacaagt tatttgtgga
     3841 tttgggcttg gaaaacattg atcgcagatt tgtaagccgt tgcgcagctc agataaataa
     3901 ggaagtggag atatggaaga accagctgct tgataatcgt tatgcttata ttatctggct
     3961 ggatgcgatt tacaccaaga ccagaacaga gggtaacgtt aactctactg cggtgttaat
     4021 agcgattggt ctcagagaag atggtcatcg cgatgtcctc ggccttcact tggggaatcg
     4081 tgaaagctat tataactgga aggactttct acagagttta aaggatcgtg gactacagag
     4141 gtcggaactc tggattagcg atgagcatga tggtttgata aagtcaatag aggaatgttt
     4201 ccctgttcag cgaaagcaac gcgatatcgt ccactggatg cgcaatgacc agagcaagga
     4261 gtccaagacg gatcttctgt ggttgttgcc actcctaaat gatctggtag gttacgggac
     4321 gaaagaatat tttcaattgg cgtggacaga gcttactagg gtagctcagt ccaaaggtaa
     4381 ggacaaactt atagattggc ttgatagcac ttaccatgag atatcgttct atcttgattt
     4441 tcctgtagca cattggagta gaatcaaatg cacaaactca ctggagcgct taaatgaaga
     4501 gctcaggcgc agagagaaat gcatcaggat cttcacagat gaaagagttg tctgcggctg
     4561 tttggtgcga tcatgcaagg ctattcagag gactggataa gcgggaaact ttatctttag
     4621 gaaccaatgg aaagaatcag aaaaaaccta aaaaagccga tcccatttga ggcagcccgt
     4681 cgcagcaaag taacttattt tgaaaaagta cttgacaggg attatcaata tgaaaataaa
     4741 gtaattagaa gcttggccat acatagattg taagtaacga gggataacag atgctctcaa
     4801 agaacctgag aaacaatgag ttggaattta caccaacatt tgggactcaa caacccaaga
     4861 agattataaa gaatagaaaa gacgtttatg ataaataaaa tcaactatat caataatatt
     4921 ggtctctttg taaatatgag aggggcttct aatattgatt taaagagatt aactttaatt
     4981 tatgctgaaa atggaagagg taaaacaacc ttatccgcta ttctaagatc tcttatgact
     5041 ggggacccta tttatattaa cgaaagacga agtataggta gtcaatccaa tccccatgta
     5101 gtactcacat gtggaggaaa tcaaactgtg caatttcaaa atggctcatg gaataaatct
     5161 atacaaggaa ttgtgattta tgatgatgtc tttgttgcca ataatgtttg ctctggtttg
     5221 atggtagatg caagtcacag acaaaatctg catgaattta taattggcga acaaggagta
     5281 aggctcaacc ggcaattata cgattacatt gagaaaatag aagcccataa tattgagctg
     5341 caaagattat ctagtttgat acctagagaa gccagatttg gacttaatat tgatgatttt
     5401 tgtagcttac aaatcgaaga aaatattgat aataagatca tggctttaga gagagacttg
     5461 ttagctgccc aaaattctgt tgaaatctcc aaagctgatc tatttactcc aattcatata
     5521 cctgagttca gccaatcagc ttattcagat cttttaggat catcattaga tactttagat
     5581 tctacggcaa tgaaactagt agaggatcat tttaataaat actcgcacaa tccagagcag
     5641 tggatttcag aaggattaga tctacttcaa tcaattcctg aagaaactga tgattatatt
     5701 tgcccattct gttctcagcc acttaaagga tcaataataa tacaacatta tcgagtttac
     5761 tttagtgcag aatacaaaca attgataggc agaataaatg aatcaattat gtttttaaac
     5821 aatcaatgtg gtgaagacaa acttattgca tttgagagag cgataagatc ttataatgaa
     5881 aatcgtcagt attggtctaa gtttactgat attcctgata ttataatcaa cagcgattct
     5941 gtcatacaaa gctggaaaaa ccttcgagat atgatttata agaaactaag taccaaaaaa
     6001 tcttctcccc tagataaagt tatttttacc gaagatgaac ttgttctgtt ctcaaaatac
     6061 gaagctagtc gtaaaaatat tgataaacat aatcggcagg ttgcccagat aaatgaagag
     6121 ctaaagacta tccaacaaag aacaaaatgc gctgatatct cagtaataaa cagtaatatc
     6181 aataggctta aggcaataaa atctcgttac tctccagatt tctcacaata ctgcgagaac
     6241 tacctaaagg agatagctaa caagcagagt actgaaattt tgagggatac agcaagaaaa
     6301 gaactacaag actatcgaga aagcatattc cctaaatttc aggaagcagt taatcactat
     6361 ctgcaagtct tcaatgctgg atttacactg tgcaatttat ctcctaccaa tattcgtatc
     6421 gggtctacat gcaattacag agtgttaata aataacattc cagtaccgat tacctcagag
     6481 cctggttcac aagcatttca taacactctc agctcgggag atcgtaattc cttggcacta
     6541 gcctttttat ttgccactct tagtcatgac cccaacctta gtaataaaat aatcattatt
     6601 gatgacccgg tttcaactct tgatgagcat agaactcttt caactgctca agagattagt
     6661 aggttatcca gtcaagccga acaagtgatt gtcttatcac acagtaaatc attcttgtgt
     6721 agtgtctggg atggaacaga tcgtattaac tgtgcatcat taaagatagt cagagaagga
     6781 accaactcaa caattaatga ttgggacgtg aatagtgatt cgttaacgga gcatgatagg
     6841 ctgttcagtt tgttagattc ctacatgaat gtagaaaatg ttaacaaaaa agaagtggca
     6901 attgctattc gtccatatct cgaaggactc ttgagagtca attatcctaa atactataaa
     6961 ccagggatga tgttaggaaa ttttattcat ttatgcaaac agaatatgaa tacacaagaa
     7021 cagatattga atgagaagca aattaatgaa ttagaaaaca ttaaggaata tgctgataga
     7081 tttcaccatg acacaaatcc aaattggcag acattaagtg taaatgatgg agaattgaga
     7141 ggatttgtaa gaagagcatt aaattttacg actatatcta attcagtaac ctaacgcgta
     7201 ggaggatcat taatgactat tcacaatgtc tcatttattg aaggccattt aaaagctata
     7261 ttatcactta tacaacttct ggctactaaa ggttataagt actcccctaa cagagagtat
     7321 aaatggcact aagcaaaaac tagtaaaaaa aaataaaaaa atatataaat gggtgttatt
     7381 gagacaggta gagggaatgc acccgtt
//
LOCUS       DAOSTH010000076         7370 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_9392_length_7370_cov_14.781135, whole genome shotgun sequence.
ACCESSION   DAOSTH010000076 DAOSTH010000000
VERSION     DAOSTH010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9392_length_7370_cov_14.781135"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1343
                     /locus_tag="PLB65_07150"
     CDS             <1..1343
                     /locus_tag="PLB65_07150"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOQ78340.1"
                     /translation="IKMLAHYHLNGILADEMGLGKTIQALSAILSTTAGKVSLVICPK
                     TLLYNWAAEIDKFHTNIPYAIVDGNKETRIEILSNPNVRLFIMSYSMVLGDIAYLKNM
                     EFEWIVLDEAQNIKNVSAQRTSAIKKLNSKHRLALTGTPIENNLTELWSIFDFLNPGY
                     LGTLNKFKQNYLPAEGEIKARLSLQKMVAPFLLRRVKKEVLLELPDKQEQISWCKLNT
                     LQEKLYLQILDMVHKKLLPEGQEMQSYIHILAALTKLRQVCNHPHLANSDILAELEAS
                     SKLEQLLELVTEATNAGHKVLVFSQFVQMLSIIRKVFDAHSLSYSYLDGQTKDRVTPI
                     KRFETNPDIKLFLISLKAGGTGLNLTAADTVILYDPWWNPMVENQAIDRTHRIGQTQK
                     VQVFRLITKGTVEEKILQLQESKRELFDTVIEGGQNMIKAMTKEDIKSLFSYSG"
     assembly_gap    1746..1845
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1900..2880)
                     /locus_tag="PLB65_07155"
     CDS             complement(1900..2880)
                     /locus_tag="PLB65_07155"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017118 - lipoyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HOQ78341.1"
                     /translation="MLSIFSPSNYAPFNIACEEYILRDFSDDVFLLYINDSSIIVGKH
                     QNTLAEINYEWVLKHNIPVVRRLTGGGTVFHDSGNLNYSFMVKENGDMNRSFERYTKP
                     ILAILHDLGVPAILEGRNDLTINGCKFSGNAKTVAFGKTLQHGTILFSSNITNLSAAL
                     NPREEKFNDKAVKSVQARVTNVSDHLPYPLSLQDFVTLIRAKVKIMYPEIQDYFLSLK
                     DKEEIQALMNSKYDTWQWNFGKSPRYNLSHSIKTQAGVIEFFLYVNKGIIEEVSIYGD
                     FFTNREISELENALRGIEHKPDTVTKVLQQMDYQSYFGEVNLDELLSAMF"
     gene            complement(2882..4603)
                     /locus_tag="PLB65_07160"
     CDS             complement(2882..4603)
                     /locus_tag="PLB65_07160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HOQ78342.1"
                     /translation="MFITDEKLKQMPYENLPVDFDVSSWEQVQAFLEELVQHDVSTID
                     NLETMLLKYSDLLKCMADELSWRYIKMTVNADDETKEQAYNDYFANIYAPAEAYQFKI
                     KKRFYESPVRTELESRKYSLLNQIFSNDIKLFREENIPLMIQESELANKYAGIVSKMS
                     AIFEGEEKTPAQLSVYLKDPDRNKRENAWRLRMNMFAEKQEELNKLYSDLIALRHQIA
                     VNADFSNYRDFKHQEMGRFSYTPEELFAFHKSVEKVVMPFIAEQNKKRKEALKLDTLR
                     PWDTAVDLDGRILKPFETTEEFINKSLKVLYQVNPEYAMQLEKMKNTGLLDLENRKGK
                     APGGYNSSINNLASSFIFMNYVKIHNDIVTLLHESGHAMHSAAMANIKIAQYLETPSE
                     VAELASMTMELLTMDYWDEFYSNPEDLKKAKRDQLEGTLSFLPWCMTVDALQHWVYLH
                     PEHSVKERYDAFVDINKRFSAGVDWTGLENYRKILWTQQLHIFEVPFYYIEYGMAQLG
                     ALSIYMNYRQDKQKALQQYQDFLNLGYTKSVKDIYKTAGISFDFSEDRIYTLVNFVRE
                     ELEKLED"
     gene            complement(4607..5011)
                     /locus_tag="PLB65_07165"
     CDS             complement(4607..5011)
                     /locus_tag="PLB65_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein GerW family protein"
                     /protein_id="HOQ78343.1"
                     /translation="MDIKDIFSQIRSMVNNAMGGGLSFGQPSKLGDLYVIPVARVIYG
                     MGGGGDQPSSEQETEKEEKTETDKAENKTKVKVAFSGGGGVGTFSKPVGLFSIKEDRI
                     KFHPVIDLMELVALGSFMTIFMIIMKKLRFIK"
     gene            complement(5150..6997)
                     /locus_tag="PLB65_07170"
     CDS             complement(5150..6997)
                     /locus_tag="PLB65_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOQ78344.1"
                     /translation="MKNFKMLICSFLLVCGGSLCGSITRYYTFDYSTAEYNPINGSQL
                     TFPDMNNYISSGIEIGFPFPYCGEMYEYVKISTNGFINPGATWNDDSPNNQLEYGIYS
                     IIAPLWDDLSLGEGNLQYATIGSAPNREFWVQYSHARWPYNQFNCYVNFQVVLSENGK
                     IEFHYGNTNGAPVNGSASIGINMPNAGAGNFFSITPGNPPLVSTTQENYSINEIIPSG
                     TVYTFNPLPPVLYDLSLEIELDLIYMNGIYHNWQESFVLPVTVKNEGTETVDSYDVVL
                     FCQQMELCRLNGISLAPAESQLYELVATIDSSGWQYLTAVAELPEDEYPEDNIDGYTC
                     IIRPNPATGIIVGAGNELQRIPIDVYWKYSLYEAIYYADEIGDSGTIYGISFYMNSTF
                     CHFLTVKVWIGETNLTNLSEGWIAAGELTPVYYSDINIPADSIEMYLPFSVPYQYHQG
                     NLCVMVYHTHTYLLTCTDPFLAQTVPEMRALKRWTDAYDPDPYNPPIYNDLYGKIPKT
                     AFYLISANDIDDEYLPIVKLLSCYPNPFSETVTLSLNLAKREQICLEIFNCKGQKVTT
                     LYNGNIEKGETKISWNGLDSNNRKVANGLYIGQLRTKERCEKVKLIVLK"
BASE COUNT         2251 a         1445 c         1267 g         2307 t
ORIGIN      
        1 ggattaagat gttggcacat tatcacttaa atggtattct tgccgatgaa atgggtttag
       61 gaaaaaccat tcaagccctc tcggctattt tatctacaac tgccggaaag gtttccctgg
      121 ttatttgtcc caaaacatta ctttacaact gggctgctga aatcgataaa ttccatacta
      181 atatccctta tgccattgtg gatggtaaca aagaaacccg cattgaaatt ctctccaatc
      241 ctaatgttcg actctttatt atgagttatt ctatggtttt gggagatatt gcttatctga
      301 agaatatgga gtttgaatgg attgttctgg atgaggcaca gaacatcaaa aatgtttccg
      361 cacaacgcac ttctgctatt aaaaaactga acagcaaaca ccgcttggct ctaacaggaa
      421 cccctatcga aaataatcta accgagcttt ggtctatctt tgattttctg aatccaggtt
      481 atttaggtac cctgaataaa tttaagcaga attatcttcc tgcagaagga gaaataaaag
      541 cccggctttc tttacaaaaa atggttgctc ctttcttact gcgtagagtg aaaaaagaag
      601 ttttattgga acttcccgat aaacaggaac aaatatcttg gtgtaaacta aatactctac
      661 aagaaaaact atacctgcaa atcttggata tggtgcataa aaaacttctg cccgaaggcc
      721 aagaaatgca aagctatatt catatcttag cagcacttac aaagcttcgc caagtttgca
      781 atcaccccca tcttgccaat agtgacatat tagcagaact ggaagcatct tccaaattgg
      841 aacaactttt ggaattggta accgaagcga caaatgcagg acataaggtc ttggttttca
      901 gtcagtttgt acaaatgcta tctattattc gcaaggtttt tgatgcccat tccttatcct
      961 atagttattt ggatggtcaa acgaaagata gagttacccc aattaaacgc tttgaaacta
     1021 atcctgatat caaactcttt cttatttctt tgaaagcagg aggtaccggc ctaaatctaa
     1081 ccgctgccga tacagttatt ttgtatgatc cctggtggaa tcctatggtg gaaaatcaag
     1141 caattgatag aactcataga attggccaaa cccaaaaagt tcaggttttt cgtttaatca
     1201 ccaaaggcac cgtagaagaa aaaatattac agctacagga aagtaaacgc gaactctttg
     1261 ataccgttat tgagggcgga cagaatatga taaaggctat gacaaaagaa gatataaaat
     1321 cgcttttcag ttactcagga tgaagaaggt agaattacag gttcagtgag tcctctttgt
     1381 ggaatggaag tagagaaggt taaagataat gggaaaaagt ggaaaagtta gacccaagac
     1441 gaacatcgta taatagatag ataataaaag tcataagtta ttgtgtagca ggagctccgc
     1501 attttgtgta gtcagagctc gctgaagctc tgcattcttt cagttattat tttttgcgga
     1561 ggaacggcgt cctccgtcta cacatcaaaa tcctaaaatc ctcaaaccct tgtttctgaa
     1621 actaaatctg tgcgaaatat cttcggtgtt ttcggtggct tataaacaaa tcctcttaat
     1681 cctaaaatcc ttaaatcctt gtttctaaaa ctcaaatctg cgtaatctgc gaaatctgcg
     1741 tgagannnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnccttg tttctaaaac
     1861 tcaaatctgc gtaatctgcg aaatctgcgt gagaaaaaat tagaacatag cgctaagcaa
     1921 ctcatccaga tttacttcgc cgaagtagct ttgatagtcc atttgttgta gcacttttgt
     1981 cacagtatca ggtttatgtt caattccgcg gagagcgttt tccagttcgc tgatttcgcg
     2041 gttggtaaag aaatcaccgt aaatactcac ttcttctata attcccttat tcacataaag
     2101 gaaaaattca atcacaccag cttgagtctt aatagaatgg gataaattat agcgaggaga
     2161 ttttccgaaa ttccattgcc aagtatcgta tttactattc atcagggctt ggatttcttc
     2221 tttatctttc aaggatagaa agtaatcctg aatttcagga tacataattt tcactttagc
     2281 tctaattaaa gttacaaaat cttgcaaaga aagcggatag ggaagatgat cagaaacatt
     2341 ggtaactcgt gcctgcacag attttaccgc tttatcgtta aatttttctt ctctaggatt
     2401 tagagcagcg ctaagattgg taatatttga actgaaaagg atggtgccat gttgcaaggt
     2461 ttttccaaag gcaacagttt tcgcgttacc ggaaaactta cagccattaa tagtaaggtc
     2521 atttctgcct tccaaaatag caggaacacc caaatcatgt aatatagcta aaattggttt
     2581 agtatagcgt tcaaaactac gattcatatc tccattttct ttcaccataa atgaataatt
     2641 caggttacca gaatcatgaa atacagttcc gccaccggtt aaccgacgca ctactggaat
     2701 attatgtttc aggacccatt cataattaat ttccgccaaa gtgttttgat gttttcctac
     2761 aatgatgctg gaatcgttaa tatagagcag aaatacatca tcagaaaaat cccttaggat
     2821 atattcttcg caggcaatat taaaaggagc atagttgctc gggctgaaga tagagagcat
     2881 tttaatcctc caatttttcc agttcttctc ttacaaaatt caccagagta taaattctat
     2941 cttcagaaaa gtcaaaagaa atccctgcag ttttatagat atcctttacg gattttgtat
     3001 atccgagatt cagaaaatct tgatattgct gtagtgcttt ttgcttatcc tggcgataat
     3061 tcatatagat agaaagcgct cccaattgtg ccattccata ttctatataa tagaaaggca
     3121 cttcaaaaat gtggagctgt tgcgtccaca gaattttacg ataattttcc agaccagtcc
     3181 aatctactcc cgcggaaaag cgtttattaa tatcaacaaa ggcatcatat ctttccttaa
     3241 cgctatgttc tggatgtaaa taaacccaat gttgcaaagc gtccacagtc atacaccaag
     3301 gaagaaagct taatgtccct tccaattgat cgcgttttgc tttttttaga tcttccggat
     3361 tggagtaaaa ttcgtcccaa taatccattg ttaaaagttc catcgtcatt gaagccagtt
     3421 ctgccacttc cgaaggagtt tccaaatact gagcaatttt gatattcgcc atggcagcgc
     3481 tatgcattgc atgtcccgat tcgtgcagta aagtaacaat atcattatga attttcacat
     3541 agttcataaa aatgaaagaa cttgccagat tgttaatcga gctgttatag cccccgggag
     3601 ctttgccttt acggttttcc aaatccagta gacccgtatt tttcatcttt tccaattgca
     3661 ttgcatattc aggatttact tgatagagca ctttcagcga cttattgata aattcttccg
     3721 ttgtttcaaa gggttttaaa atgcgcccat ccaagtccac agcagtatcc caaggtctta
     3781 atgtatccag ttttaatgcc tcttttcttt ttttgttttg ttccgcaata aaaggcatca
     3841 ccactttctc cactgactta tgaaaagcaa aaagttcctc tggagtatat gaaaaccgcc
     3901 ccatctcctg atgcttgaaa tcacgataat tgctaaaatc tgcatttaca gcaatttgat
     3961 gacgcaaagc aatcagatca gaatagagct tattcagttc ttcctgtttt tcagcaaaca
     4021 tattcattct caggcgccat gcgttttccc gtttgtttct atcaggatct ttcagataaa
     4081 cggaaagttg tgccggggtc ttttcttcgc cttcaaaaat agcactcatt ttgctgacaa
     4141 tcccggcata tttatttgcc aattccgatt cctgaatcat caaggggata ttttcctcgc
     4201 ggaaaagctt tatatcatta ctaaatattt gattcagcaa actgtatttt ctggattcca
     4261 attctgtgcg gacagggctt tcataaaaac gctttttaat tttaaattga tatgcctctg
     4321 cgggagcata aatattggca aaataatcgt tatatgcttg ttctttagtt tcgtcatcgg
     4381 catttacagt catttttata taacgccagg atagttcatc tgccatacat ttcagcaggt
     4441 cagaatattt taataacata gtttccaaat tatcaatagt gctaacatca tgttgcacaa
     4501 gttcttctaa gaatgcttga acttgttccc aagaagagac atcaaaatcc acaggcagat
     4561 tttcgtaggg catctgcttt aacttttcat cagttatgaa catttcttac tttataaacc
     4621 tcaacttctt cattattatc ataaatatag tcataaaact tcctaaagcc actaattcca
     4681 taagatcaat tacaggatgg aatttaattc tatcttcttt aattgaaaat agacctactg
     4741 gcttactaaa agttcctacg cctcctccac cactgaacgc aactttcacc tttgtcttat
     4801 tttctgcttt atcggtttcg gttttctctt ctttttcagt ttcttgttca gaagagggtt
     4861 ggtcgcctcc accacccata ccataaataa ctctggcaac cggaatcaca taaagatctc
     4921 ccaatttaga aggttgccca aaagataaac caccgcccat agcgttattt accatagagc
     4981 gaatttggga aaagatgtcc ttaatatcca tagccaactc cttattcata tctctatttt
     5041 atatattatg ttttttttgt caagcgacaa ttgcactttt cgctaaattg gataccagaa
     5101 gtctgttaat tatctttgcc taaaactggt tataagttag cttgaggttc tatttcaaca
     5161 ctatcaattt taccttttca caccgctctt ttgttctcag ctgaccaata tacaagccat
     5221 tggcaacttt cctgttattg ctgtctaaac cattccaaga tattttagtt tcgccttttt
     5281 ctatattgcc gttatatagg gttgtaactt tttgcccttt gcaattgaat atttctaagc
     5341 agatctgttc tcttttagcc aaatttaagc ttagggttac agtttctgaa aagggattag
     5401 gatagcagga gagtaattta acaatgggca gatattcatc atcaatatcg ttagctgaaa
     5461 ttaaataaaa agccgttttt ggaatcttac catataagtc attgtaaatg ggaggattat
     5521 aaggatcagg atcgtaggca tcagtccagc gttttagagc tctcatttct ggaaccgttt
     5581 gagccaaaaa aggatccgta caggtaagta gataagtatg agtatgatat accattacgc
     5641 acaaattgcc ctgatgatac tgataaggga ctgaaaatgg cagatacatt tcaatgctat
     5701 ccgcaggtat gttaatatcg gaataatata ctggcgtcaa ttctcctgca gcaatccagc
     5761 cctcgcttaa atttgttaaa ttggtttccc ctatccaaac ctttactgtc agaaagtgac
     5821 aaaaagtgga attcatataa aaactaatgc cataaatagt tccgctatca ccaatttcgt
     5881 cagcatagta aattgcttcg taaagtgaat atttccagta aacatctatg ggtattcttt
     5941 gaagttcatt tcccgcacct acaatgatgc ctgtagcagg attagggcgg ataatacagg
     6001 tataaccgtc aatattatcc tccggatatt catcttcggg aagttctgct accgctgtta
     6061 aatattgcca tccactggaa tcaatagttg ctactaattc atataattgg ctttctgcag
     6121 gtgccagaga aataccattt aaacgacaaa gctccatttg ctggcaaaaa agaaccacat
     6181 catagctatc aactgtttcg gttccttcat tttttactgt taccggaaga acaaaacttt
     6241 cttgccaatt gtgatatatc ccattcatat aaattaaatc taattctatc tcaagcgaaa
     6301 gatcataaag cacaggaggt aatggattga aagtgtaaac tgttccggaa ggaataattt
     6361 cgttgatact ataattttct tgcgtagtag aaaccaaagg aggatttcca ggagtgatac
     6421 taaaaaaatt gcccgctcca gcattaggca tattaattcc gatggacgca gaaccattta
     6481 caggagctcc attcgtgttc ccataatgga attcaatttt accattttcg gaaagcacta
     6541 cttgaaagtt tacataacag ttaaattgat tatagggcca gcgagcatga gagtattgaa
     6601 cccaaaattc acggttggga gcagagccaa ttgttgcata ttgtaaattg ccttccccca
     6661 aactaagatc atcccaaagg ggagcgatta tagaataaat gccatattct aactgattat
     6721 tagggctgtc atcattccaa gtggcacccg gattgataaa accattagta gaaattttaa
     6781 catattcata catctctccg caatagggaa aaggaaaacc gatttctatg ccagaactga
     6841 tataattgtt catatctgga aaagtaagtt gacttccgtt tataggattg tattcagcag
     6901 tagaatagtc aaaagtataa tagcgggtaa tggagccaca caagctccct ccgcagacaa
     6961 gcagaaatga acaaattagc attttgaagt ttttcatcgt ccatctcctt ttggaaagga
     7021 accgaaacct aatattctca tttttcttta tattatttct tcaaaactaa gtcaagcatt
     7081 ttttatttct ttattttctt attacttact tacagacatc tcctcttcct cccgtaatca
     7141 actcctcctc cactcgtcat taaggatgag taaaggttgg gtgaattaag tgaggggaaa
     7201 aagggagaga gaataactag atgatagatg aagaactaag aatgtctatg gtttcgccgg
     7261 cttaaaaccc gtcgctcaaa tctgtcgtcc cgctgggact tttttatccg ttctcttttt
     7321 tcgccgggtt aaaacccgtc gttagtatgt atcgttccta acggaactaa
//
LOCUS       DAOSTH010000077         7317 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_9495_length_7317_cov_9.462545, whole genome shotgun sequence.
ACCESSION   DAOSTH010000077 DAOSTH010000000
VERSION     DAOSTH010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9495_length_7317_cov_9.462545"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..900)
                     /locus_tag="PLB65_07175"
     CDS             complement(<1..900)
                     /locus_tag="PLB65_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78345.1"
                     /translation="MPFVLSGATLYVALDGSQAYSSIQSAVNAAADQDTILIYPGTYY
                     ENIEIIGRNLSIGSLEFTTADSTYIAQTVIDANQSGSCFNITEDSEITLQGLYLTNGI
                     GTPSFNWGDRKGGAVCVSESNINIINCQLIGNEAWTGAGVYLLHSFAYLAGTVITDNW
                     EKCFGALTFAGYSLDGPRTLEFDPINRCSIYNNIAGSCGNDITIISQFYNSIDIYLDK
                     ATVAANSPYLKECIYTEQYDTDQEFTYNAFINEAVTQQQFADLYVSSEGDDSNSGLTP
                     QTALKTIDLGADWDQNAPYNNYCP"
     gene            complement(1042..3207)
                     /locus_tag="PLB65_07180"
     CDS             complement(1042..3207)
                     /locus_tag="PLB65_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78346.1"
                     /translation="MKKEYKLLKRSEWRIILLFIALALISSVLDAINTWDASIEYLSL
                     IERIENDHRQHQRIDIGDIRMGKNNDNYSPSPFREKHWLFYDSKNLYYAVIAEIDSSF
                     SRGVEAVRDGNTASDNIYLQLITQPESNLAYVYGFSPLGCKMDYTIDYSFGSNYNWDS
                     SYQYKSELHNNLWFIEATIPFNDLRYSGAPPYEFSVMFKRTHYKSMSYYRYPYVDSKI
                     GLDYFRSFYPITIETHIEHQWNPKLKLHSTMVYDLQEEQTNDLADYTGLNFSLKPGQN
                     STAKLTIQPDFSDTPLDSESNVYNSKYPPTIEENRSFFIEDYDLLAPNADFWYTRKII
                     SPIIALKFNRIQNNSALAVLALKDKENSDVMDSGDYWLAAGYSNNVGNNQVIFNAYTR
                     GSEDWADYNSLVYLITGIRPGKNILVNPEFGFSFDHKPSTDKVGTYGKLAIAWQGEHF
                     YLMGGSEYLSRDFTASMGSINETDRANLVAQIEYQKYFDTYLNYLTSQLTMSTTYNLK
                     FDTNYYRSLIFSTSATGFNNVLTLSYNTDYSEEYFSYIMHSTYAHTLGLSSSKYQYLM
                     PSASFTSGKSIIYSQNRTADYFSFVPTIWTEINQNTTLGLGIYYIKYNAKQTDVFDNE
                     YYFSNINLKTQFVDKISITQGIRINNYTNTMVPASDVSSAIFKNGYIGYYVNIDWMVT
                     KHFNLITGYKSIESRYRMDEERELAVEDQNMYLKVEYQF"
     gene            complement(3495..4586)
                     /locus_tag="PLB65_07185"
     CDS             complement(3495..4586)
                     /locus_tag="PLB65_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOQ78347.1"
                     /translation="MKEIGSIAMDKNLYNEIVRYISELTLQRFYYLNLADDIANSILV
                     SFMQSSTSTDPYEHTKDYIKSVFPKAVEDVFSYYQKLTLQYCLTKTKDHSVSEDISQE
                     AIRQLLSSKNKINDVYSWLRQVTHNLICKHYQFKANEKDLHNLLYMETSSLQNVMESG
                     TTIDIEGLDPIKKKEILASQEYRDYCAMLSFDSIKEYASSMNVSEKVAQKRKERITRN
                     IRSKILLAIGWEASHEILSYNQYNAIQKFIRELLKIGCNGEDIKQNSKLNPKLTQVMN
                     GIERIDDWGITMVDNCKFQLHIFHLNAEKQPIIATFYITLNERNHISVENCKKNEIIG
                     VHPIPANILIPKEKGKSLWSYEKIISLLK"
     gene            complement(4903..5937)
                     /locus_tag="PLB65_07190"
     CDS             complement(4903..5937)
                     /locus_tag="PLB65_07190"
                     /inference="COORDINATES: protein motif:HMM:NF024706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HOQ78348.1"
                     /translation="MEQKNMPAKSEFLLYQSKDGKLRMETRLENETVWLTQKQLAELF
                     QTTVANINIHIKNIYGEGELEPDRTIKDFLIVQNEGEREVQRQVTHYNLDMIISVGYR
                     IKSTIATHFRQWATQRLREYIIKGFTLDDERLKQMGGGNYFDELLARIRDIRSSEKIF
                     WRKVLDIYATSIDYDPRAEVSRDFFKVIQNKMHWASHGHTAAEIIYARADAQKPYLGL
                     TSWSGDQPRKADTEIAKNYLNEQELDILNRIVTMYLDFAELQALNRKPMRMQDWIIKL
                     DEFLKLSGRDILKHSGKVAHEEALQKARLEYDKWQQEQLTHPTEVEKHFVEVIEEVKQ
                     LEKKSPKRKM"
     gene            complement(5939..6184)
                     /locus_tag="PLB65_07195"
     CDS             complement(5939..6184)
                     /locus_tag="PLB65_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78349.1"
                     /translation="MITDYHAKYYAYELSRMGGAGVNRVGRALFDACVDLNPHQIEAL
                     SQRMKQKTKVANLFTIHWKVRLIEEEFLKRMWSAEGV"
     gene            complement(6664..>7317)
                     /locus_tag="PLB65_07200"
     CDS             complement(6664..>7317)
                     /locus_tag="PLB65_07200"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78350.1"
                     /translation="TPYEVAMFAFNESTFADVSFNNCLVSGGSDSFFILGGDITHTWM
                     DGNMFGYPSFLGGDTNNPLYYSLASNSPCIDSGTPDTANLDLPPYDLAGNWRIWNGRI
                     DMGCYEYDSEPWVDNDDPFLPQPESTILLQNYPNPFNPTTTISYQIPKSGSVKLEVYN
                     LKGQLVKTLIDDTQNSGIHGIIWNGTDSHNRNVASGVYLYRLSSINNVQTKRMLLMK"
BASE COUNT         2089 a         1449 c         1340 g         2439 t
ORIGIN      
        1 aggacaatag ttgttataag gtgcattctg atcccaatca gcacccagat cgatcgtctt
       61 cagtgcggtc tggggtgtta aacctgaatt cgaatcatcg ccttcagaac ttacatacaa
      121 atcggcaaat tgctgctgag taaccgcttc attaatgaat gcattgtagg taaactcttg
      181 atcagtatca tattgctctg tataaatgca ttctttcaag taaggggagt tggcggctac
      241 tgtggcttta tccagataga tgtctataga attataaaac tgcgaaatta ttgtgatatc
      301 attgccacaa ctgccggcaa tattgttgta tatactacac cggttgatag gatcaaattc
      361 cagtgttctg ggaccatcca gagaatatcc cgcaaatgtt agtgctccaa agcatttctc
      421 ccagttatca gtaataaccg ttccggcaag gtaggcaaag ctatgaagca aataaacacc
      481 ggctccagtc cacgcttcat tgccaatcaa ttggcagttg ataatattga tgttactttc
      541 tgaaacacat acagctccac ctttcctgtc cccccaattg aagctcggag tgccaatgcc
      601 attggttaga tacaagccct gcagcgtaat ctcagaatct tcagttatgt tgaagcaact
      661 tccactttga ttggcgtcta tcaccgtttg tgcgatgtat gtgctatccg cagtagtgaa
      721 ttccaagctc ccgatgctga ggttcctgcc aataatctca atattctcat agtaggtgcc
      781 ggggtagatg aggatagtat cttgatccgc agcggcatta actgcacttt gaatagatga
      841 atatgcttgc gagccatcca aggcgacata gagagtcgcc ccactaagaa cgaacggcat
      901 aagaagcagg accatcaata gagttttcat attatcctta atagtcaaaa tggaagcaag
      961 catcaatttg gatgaccgct tccgaatata tggaaatagt attttagagt gtgaatagaa
     1021 gttgttaatg atgctaacca tttagaattg gtattccact tttagataca tattctggtc
     1081 ttctacagct aattctctct cctcatccat tctatatctg ctttctatgg acttatatcc
     1141 ggttattaaa ttgaaatgtt tcgttaccat ccagtcgatg ttaacataat atcctatata
     1201 gccatttttg aatatcgccg aggacacatc tgaagctgga accatagtat tagtatagtt
     1261 attaatcctt atcccttgcg taatggatat tttgtcaaca aactgagttt tcaaattgat
     1321 gtttgaaaaa taatactcat tatcaaaaac atctgtttgc ttggcattat acttgatata
     1381 gtatataccc aatcctaatg tagtgttttg attgatctca gtccatatag ttggtacaaa
     1441 actgaagtaa tctgcagtcc tattctggct gtagataata ctttttccgg atgtaaaact
     1501 tgccgatggc attaaatatt ggtatttgga agatgataat ccgagagtat gagcataggt
     1561 tgaatgcata atgtagctga aatattcttc cgaatagtca gtgttgtagc ttagggtaag
     1621 gacattatta aatccggtgg cactggtaga aaatattaaa ctccggtaat agtttgtgtc
     1681 aaatttaagg ttatatgttg ttgacattgt caattgggat gtaagataat tcaagtaagt
     1741 atcaaaatac ttttgatact caatttgcgc tacaagattc gctctatcgg tttcgttgat
     1801 tgagcccatg ctcgcagtaa aatcacggct gaggtattca gaaccaccca taagataaaa
     1861 gtgttcacct tgccaggcaa tggctaattt accataagtc ccgaccttgt ctgtgcttgg
     1921 tttatggtca aaagaaaaac caaactctgg attaacaagt atatttttcc ctggtcgtat
     1981 gccagtgatc aagtacacca gagaattgta atcagcccag tcctcagagc cgcgagtata
     2041 ggcattgaaa ataacttgat tattaccgac attatttgaa tatccggcag ctaaccaata
     2101 atctccagaa tccatcacat cgctattttc tttatccttc aacgccagaa cagctaaagc
     2161 gctattattc tgtattctat tgaacttcaa tgctataatg gggctgatta tttttcgggt
     2221 ataccaaaaa tctgcatttg gtgccaatag gtcatagtcc tcaatgaaaa aactgcgatt
     2281 ttcttctatc gttggtgggt acttgctgtt atacacatta gattcagaat caagaggtgt
     2341 atctgaaaaa tccggttgga ttgtgagttt ggcagtgcta ttctgacctg gttttaagct
     2401 gaagtttaac ccagtataat cagccaaatc atttgtctgt tcttcttgca aatcatagac
     2461 catagtggag tgtaatttaa gcttaggatt ccattgatgt tcgatatgag tttctatcgt
     2521 tattggataa aaacttctaa aataatctaa tccaattttt gaatccacat aaggatacct
     2581 atagtaagac atagatttgt agtgtgttct tttaaacatc acagaaaact catagggtgg
     2641 agctcccgag taccgaagat cgttaaaagg gatcgttgcc tcaataaacc ataagttatt
     2701 atggagttcc gatttgtatt gatagctgct atcccaatta tagttagatc caaagctata
     2761 atctattgta taatccattt tacacccaag cggagaaaaa ccataaacat aagcaaggtt
     2821 tgattctggt tgggttataa gttgtaagta aatattatca ctggcagtgt tgccatctct
     2881 gacagcttca actcctctac taaagctgct atctatttct gcgattacgg catagtaaag
     2941 atttttagaa tcataaaaca accaatgctt ttccctgaaa ggggatgggg agtagttatc
     3001 attattttta cccatacgaa tgtcaccaat atctatgcgc tggtgttgcc gatgatcgtt
     3061 ttcgattctt tcgatcaagc tcaaatattc tattgaagca tcccaagtat tgatagcatc
     3121 taacacgctt gagattagag caagggcaat aaataatagt atgattcgcc attcacttct
     3181 ttttaatagc ttatactctt ttttcacttt tttctcccat aaagtagata aatatccacc
     3241 ccgcacgatg aatattatcg attcggaact tcgtaaacaa ttttaacgat agaatcattt
     3301 aatgaacctt tatagacggg aagagtttgg tgctcaatgg ctgtgcagta gataactgca
     3361 caaaacagat taagacacac tacagccaac actatgtaaa gaattagttt cttaatttta
     3421 acgctccttt gaatttttct ctaatatata agtgtcaaaa ttgagaaaat tgagatgcca
     3481 gaattgaaga tattttactt cagaagtgaa ataatttttt cgtaagacca gagtgattta
     3541 cctttctcct ttggtatcag tatgttagca ggaattgggt ggacaccgat gatttcattt
     3601 tttttgcagt tttctactga gatgtggttt ctctcattca aggttatata aaatgtggct
     3661 atgatcggtt gcttttcagc gtttaaatga aatatatgga gctgaaattt gcagttatct
     3721 accattgtaa ttccccaatc atctatcctt tctatgccat tcataacttg tgttagcttt
     3781 ggatttagct tactattttg cttgatatcc tcgccattac aaccaatttt tagaagctcg
     3841 cggataaact tctggattgc gttgtattga ttgtagctaa gtatctcgtg gcttgcttcc
     3901 cacccaattg cgagtagtat ctttgaccta atattgcggg tgattctctc ttttcttttc
     3961 tgtgccactt tttcactaac attcatagaa gacgcatatt cttttatact atcgaatgat
     4021 agcattgcac aataatcacg atattcctga ctggcgagaa tttccttctt tttaatggga
     4081 tccaatccct ctatatctat ggtagttcca gattccatca cattctgtaa agaagaggtt
     4141 tccatatata gtaagttgtg caaatctttt tcgtttgctt taaactggta gtgcttacag
     4201 attagattat gcgttacctg tcttagccaa gaataaacat cattgatttt attctttgat
     4261 gataatagct gccttatagc ttcctgtgaa atatcttcgg atacactatg atctttagtt
     4321 ttcgtaaggc agtattgcaa tgtcaatttc tgatagtagc taaatacatc ctctactgcc
     4381 ttcgggaata cggacttgat gtaatctttt gtatgctcgt aaggatcggt ggaggttgag
     4441 ctttgcataa aacttactaa aatggaattg gcgatatcat ctgccagatt aagataatag
     4501 aatctctgta aggtcaattc acttatatat cttacgattt cattatataa atttttgtcc
     4561 attgcgatag agccgatttc tttcatttta ttatcctaag gagcagtttc actaagaaca
     4621 atattaagtt ccttaattga tctgtaattt ctaatgtata gtcgggataa atacattcaa
     4681 cacctctctt gtaagataga ttatattatt gaactaatca tactatctat gtaattaggg
     4741 tcttccataa aaaccacatt ttcctgcaga tactcgtctg gatcataacc tcggagcttg
     4801 agctcctgtt taggagtccc tgacaatatc cagcaactaa ggatgacttt gggatcagtt
     4861 tattggtgaa tttatgtgtt tttagagtag ttttgcgtgc cattacattt ttctcttggg
     4921 gcttttcttt tccaattgtt tcacttcttc aatcacttcc acaaagtgct tttccacttc
     4981 agtcggatga gtaagctgtt cctgctgcca tttgtcatat tccagacggg ctttttgcag
     5041 tgcctcttca tgagctactt taccactatg tttcaagata tccctaccgc tcagtttgag
     5101 aaactcatcc agcttgataa tccaatcctg catccgcatc ggtttcctgt ttaatgcctg
     5161 taattccgca aagtccagat acatagtgac aatccgattc aggatatcca gttcttgctc
     5221 attcagataa ttcttggcta tttcggtatc tgccttccgg ggctgatctc cactccaact
     5281 ggtgagtccc agatatggtt tttgggcatc tgctctggca tagatgattt ccgctgccgt
     5341 gtgtccatga cttgcccagt gcatcttgtt ctggatgacc ttaaagaaat cccgagaaac
     5401 ctccgctctg ggatcataat ctatactggt ggcatatata tccagcactt tacgccagaa
     5461 tatcttttct gaggagcgga tatccctgat gcgggcaagc aactcatcaa agtaattgcc
     5521 accccccatt tgcttcaagc gttcatcatc caaagtaaag cccttgatta tatattccct
     5581 taacctttgg gttgcccatt gccgaaaatg agtggctatg gtagatttga tccggtagcc
     5641 cacactgata atcatatcca gattgtaatg tgtaacttgc cgctggactt ctctctcacc
     5701 ctcattttga actattaaga aatccttaat agttcgatcc ggctccaatt caccctctcc
     5761 atatatgttt tttatgtgga tattgatatt agccacagtg gtttgaaata gctctgccag
     5821 ttgcttttgg gttaaccaca cggtttcgtt ttccagccgg gtttccatcc tcagcttgcc
     5881 atctttgctt tgataaagta aaaactcgct ttttgcgggc atattctttt gttccatctt
     5941 acaccccctc tgcactccac atccgtttca aaaattcttc ttcaattagc cgaactttcc
     6001 agtggattgt gaaaagatta gcaaccttgg ttttttgttt cattcgctga gacagggctt
     6061 caatctgatg gggatttaga tcaacacagg catcaaatag agccctacct actctattta
     6121 cgcctgctcc tcccatacgg ctgagttcgt atgcatagta ctttgcatgg tagtcagtaa
     6181 tcattcgcct gcctataata actatattat tgtgttatcc tataaatcta tctgcttttg
     6241 gatgtgaaaa tgataacaat actactttta gattattatc attctgtcaa tcacaaattc
     6301 agttgagtcc taaatattag tgtcctttta actcattgtt tctcaggttc tttgagggca
     6361 tttaatagac ctcgtaactc actatctata ttaagttata cgacaaattg tataattttc
     6421 atcttttcaa tatatgtcaa gttctttttc ataaaaaaat gggagggtaa agtaaaaagt
     6481 atcctccctt attttctttg cctatcaggt gcaaatattt gtaaaccacc ataaaagtta
     6541 tctcaggatt aggttcattg gcattcaatt cagatgagaa gtttgaaata aaagtgtcat
     6601 aaagtatgct gtaaacacaa ctaactgcaa aagaagcgga aacccgcttc ctatccttaa
     6661 attctatttc attagtagca ttcttttagt ttggacatta tttatgctgc taagacggta
     6721 gagatatact ccgctggcaa cattgcggtt atggctatca gttccattcc atataatacc
     6781 atgaatgcca gaattctgag tgtcgtcaat cagggttttt accaattgtc ccttcaggtt
     6841 atagacctcc aattttacag atcctgattt gggaatctga taagaaatgg tagttgtggg
     6901 attgaaggga ttggggtagt tctgtaagag gatggtgctt tctggctggg ggagaaatgg
     6961 atcgtcatta tctacccaag gttcagaatc gtattcatag caacccatat ctatccttcc
     7021 attccatatt cgccaatttc ctgctaaatc gtaaggaggc agatcaagat ttgctgtgtc
     7081 tggtgtaccg gaatctatac aaggcgagtt tgaagccaga ctataataca aaggattatt
     7141 agtatcccct cccaagaaag acggatatcc aaacatgttg ccatccatcc aggtatgagt
     7201 gatatcccca cccaggataa agaagctatc gctaccacca ctaacaaggc agttgttaaa
     7261 tgaaacatca gcaaaagtag attcgttaaa agcaaacatc gccacttcat aaggcgt
//
LOCUS       DAOSTH010000078         7223 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_9637_length_7223_cov_12.566546, whole genome shotgun sequence.
ACCESSION   DAOSTH010000078 DAOSTH010000000
VERSION     DAOSTH010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7223
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9637_length_7223_cov_12.566546"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="PLB65_07205"
     CDS             complement(<1..781)
                     /locus_tag="PLB65_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase"
                     /protein_id="HOQ78351.1"
                     /translation="MLQLDDLQISIAVSGLKTGDNPQPGIPVIRSLRAAGFKGKIIGF
                     VYDAMESGIYLEDIADEIYQMPYPSTGAESFLTRLGYINSRSKIDVIIPTLDSEIPLY
                     IRLQKELQELGIHTFICTEKQFNLRDKSKLFQYFTSEDITVPKTVLLNSVAEINKAIQ
                     DIEFPVFIKGHLYEAYKANNIIEAQKYFLELQAKWGLPVILQELIEGDEFNVVILGDG
                     KGNCLGMVPQRKLVITDKGKGFGGVVVNNPALEKFARKIIQI"
     gene            complement(787..1035)
                     /locus_tag="PLB65_07210"
     CDS             complement(787..1035)
                     /locus_tag="PLB65_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOQ78352.1"
                     /translation="MNTEQLMTLAVNKNGFVFDPENGISYTVNETGLFILQKLQSGVT
                     KEEIIDFLTEEYEVTAKNAESDLDHFMAMLKALNLIEE"
     gene            1480..3021
                     /locus_tag="PLB65_07215"
     CDS             1480..3021
                     /locus_tag="PLB65_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOQ78353.1"
                     /translation="MVGIVLSYTTLGIDAYQISVEADIGAGFNPMNIVGMASNAVKES
                     KDRVLAAIKNAGYQISTQHYTINLAPADLKKDSAALDLPIALAILECNKIFQIIAYDK
                     IAVIGELSLDGYVRPVSGVLPIALAAKKDGIDTLIVPLENAEEAAIIEGLQVIPVTSL
                     KECVGWLCNEIKIQAAKVDRDKIFQVLNDFPVDMCDVKGQFQVKRALEVAAAGGHNVL
                     MIGPPGSGKTMLARRVPTILPELNLEEALEATKIHSVAGYSKNFRNGILTTRPFRSPH
                     HTISDVALIGGGAFPKPGEVSLSHRGVLFLDELPEFKRVVLEVLRQPLEDGVVTISRA
                     ASSLTFPAEFMLIASMNPCPCGYYGANIPNHQCTCEWGSILRYRSRVSGPLLDRIDIH
                     VEVPTVSYADLASLPTGDKSSDIRNRVNTARTIQYERFKGKGIFNNSQMNSRQLRKFC
                     ILDDECNTLLQNAIDKMGYSARVFDRILKVSRTIADLDGRQNILPDDISEAIQYRTLD
                     RKYWS"
     gene            complement(3195..4316)
                     /locus_tag="PLB65_07220"
     CDS             complement(3195..4316)
                     /locus_tag="PLB65_07220"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ78354.1"
                     /translation="MIKRFITSRILNNLHKGKAILIMGARQSGKTTVLKGLFPAQPDV
                     LYLNCDDLEDRNLLAAETISSMKQFAGKSKYILIDEAQRVQNIGLKLKLLVDNFPEVQ
                     IIATGSSSFELSNQIMEPLTGRKYEFYLYPFAYQELADNFGEYQERKELANRLIYGSY
                     PDIVTHPSEAATLLNHLVGSYLYKDIFTFQDLRKPELLDRLLNALALQIGSEVSYNEL
                     AQLISTDQKTIQKYIQLLEQAYIIFRLPNYSTNQRNEIKRSRKIYFWDNGVRNCLIND
                     FRDIAVRDDIGKLWENYIVSERMKLLSNAGILRRSYFWRNIHKSEIDYLEVYEDKITA
                     YEIKWNPRRNFKATAFTSLYPKAGLFFINPENYSEFLYV"
     gene            4864..7077
                     /locus_tag="PLB65_07225"
     CDS             4864..7077
                     /locus_tag="PLB65_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HOQ78355.1"
                     /translation="MKRLYIIIVILIISICLWAQPFELDNNVFNPSGVPSFSFSQPRF
                     MDIDGDGDYDFWLGNTNRAPLYIQNNGSATNPAFSPGLDLLSSISYLAAEVSVSADMN
                     GDGILDLVTGGFTGLHLFLNSGTNASPVFTESGGYFSGLNTVMNPVPDVADVDNDGDL
                     DLVVGFSEDGRVNVYFNSGTAVSGQFSEDNCLLLADIGLYAYPIFCDFDLDGKQDIFC
                     GRDSYGFVYFQNTGTATNPVWEENNALFAGLGMSTYWNSPDLVDLNGDGQLDLVYGTA
                     DGPLQYYVNNGTAQNPSWQENTSLFGGVLDVDGASNPVFYDFDGDGDYDIISGSQMGD
                     IKFYRNTGTPYSPAWQADNAFFASIDHSIYSAVAVGDVDADGFPDVIVGDLNGGLYFH
                     HNTGTGLIEQTGILPAVSVGGWSCPRLVDMDFDGDLDLVVGNEAGNIFYYQNNGTPVS
                     PSWQVVNGFFGTIDVGSDCVPTFGDLDADGDLDMVTGNLFGEVQCFLRQRQIWEENNV
                     LFNGIETDQNATPALVDLDHDGDLDLVLGDYDGTFKFFRNLKYSGAVLNPPLNPSAEI
                     NNEIMVLWDNPTTGSTSPFEHYNIYLDEVFCGSTNDNFWIFSGLETNVTYLVSITAQY
                     IAGESMPVTMEVIILGNEDPVLKPYTLTNFPNPFNPSTQIQFSLSKAEDVSLTIHNIK
                     GQLVKTMYLGKAESGMHTLTWNGKDESGNLCAGGVYFYKLQTKDSVLVKKMLMLK"
BASE COUNT         2197 a         1236 c         1431 g         2359 t
ORIGIN      
        1 gaatctggat tattttacga gcaaactttt ccagagcagg attgtttact actacgcctc
       61 caaaaccttt gcccttatct gttataacca gttttcgttg tggaaccatt cctaaacagt
      121 ttcctttgcc atctcctaaa ataacaacat taaattcatc accttcaata agctcttgca
      181 gaataaccgg taagccccat tttgcctgaa gctccagaaa atacttttga gcttctataa
      241 tattattggc tttatatgct tcgtagagat gacctttaat aaaaactgga aactctatat
      301 cttggatggc tttattgatt tctgcaacac tatttaaaag gactgtttta ggaaccgtaa
      361 tatcttcaga tgtaaaatac tgaaacagct tgcttttatc gcgcaggtta aattgcttct
      421 ctgtgcagat aaaagtatgt ataccaagtt cttggagctc tttttgtaaa cgaatataaa
      481 gcggaatttc agaatccaaa gtagggataa taacatcaat tttacttctg gaattgatat
      541 aacccaagcg ggttaaaaat gattccgcac ccgtggaagg ataaggcatc tgataaatct
      601 cgtctgctat atcttccaga tatattccgg attccatagc atcataaaca aagccgatta
      661 ttttaccttt aaaaccagca gctctaagcg aacgaattac tggaattcca ggttggggat
      721 tatcacctgt ttttaaccca gatacagcaa tgctaatctg tagatcatct aattgtaaca
      781 ttgctcttat tcctcaatca aattcaaggc ttttagcatt gccataaaat gatctaaatc
      841 actttcagca ttctttgcag taacttcata ttcttcggtt aagaagtcga taatctcttc
      901 ttttgtaact cccgattgta atttctgcag aataaaaagg cctgtttcgt ttacagtata
      961 gcttatccca ttttcaggat caaaaacaaa gccattttta tttacagcaa gggtcataag
     1021 ttgttctgta ttcatttttt cctcagcatt tagtttgcag gatataataa tatgtatccc
     1081 gattatccta actaatatgc aattcttatg ccaattatct ttaagaaaaa agataataag
     1141 aagaagattt tggaagtact aggctgagaa cagataactg cataataact attactttat
     1201 aaccaattat cgcttatggg attaagggca taatcggttg ttgcttcaat agtaagtatc
     1261 acttttaaat cccaaataag ttttaggatt gtgaaaagag atttacaggc agcgatcact
     1321 tccaaatttt taagatctct aagatctccg tattcccgtg taaaccatac ctattcaatc
     1381 tgtaaatcaa gcgaagtctg tttgagaaga tctttttcct tgactttgta actttctact
     1441 tttttgatgg cctgatgtaa aatattaagg aaaaaggata tggttggaat tgttctttca
     1501 tacactactc ttggaataga tgcttatcag atttctgtgg aagctgatat tggagctggt
     1561 tttaatccta tgaacattgt aggaatggct tctaatgcag taaaagaaag caaagataga
     1621 gttttggctg cgataaaaaa tgccggttat caaatcagta ctcaacacta tactatcaat
     1681 cttgctcctg cagatttaaa gaaggatagt gctgctttgg atttaccgat tgccttagct
     1741 attttggagt gcaacaaaat ttttcagata atagcttacg ataaaatagc ggttatagga
     1801 gagctttctt tagatggcta tgttcgtcca gtgtcaggtg ttttgcctat tgctttagct
     1861 gccaaaaagg atggaattga tactctcatt gtccctttgg agaatgcaga agaagctgct
     1921 ataattgaag gacttcaagt aattcccgta acttctttaa aggaatgtgt cggttggctt
     1981 tgtaacgaaa taaaaataca agctgccaag gttgatcgag ataagatatt tcaagtgttg
     2041 aatgattttc ctgtagatat gtgtgatgta aaaggtcagt ttcaagtaaa acgggcatta
     2101 gaagttgccg ctgctggagg tcataatgtt ttgatgattg gtccccccgg aagtggcaaa
     2161 acaatgttgg cacgtcgagt tccgacaatt ttaccagaat tgaatctgga agaggcattg
     2221 gaggcaacga aaattcattc tgtagctggc tattccaaga attttaggaa tggtatttta
     2281 actaccagac catttcgctc accccatcat acaattagtg atgttgcttt aattggaggt
     2341 ggtgcatttc ccaaaccggg agaggtttct ctctctcata gaggagttct atttttggat
     2401 gagcttccgg aatttaaaag agtagtttta gaggtcttga ggcaacctct ggaagatgga
     2461 gtagtaacaa tttcccgcgc agcttccagt ttaactttcc cggctgaatt tatgctaata
     2521 gccagtatga atccctgtcc ttgtggatat tacggtgcca atattcccaa tcatcaatgc
     2581 acttgtgaat ggggttcaat tttgcgttat cggagcagag tttctggtcc gcttttagat
     2641 agaattgata tccatgtaga agttcctacc gttagttatg cggacttggc ttctttacct
     2701 actggcgata aatcttctga tattagaaat agagtaaata cagctcgtac cattcaatat
     2761 gagcgtttca agggaaaggg catatttaat aacagccaaa tgaactcccg ccaattgcgc
     2821 aaattctgta ttctggatga tgaatgtaat accctactgc aaaatgcgat agataaaatg
     2881 ggctattcag caagggtctt tgatagaatt ctaaaagttt ccagaaccat agcagatctg
     2941 gatggacgcc agaacatact tcccgatgat atcagcgagg caattcaata ccgtactctg
     3001 gatagaaaat attggagttg aaaatataga gagaatctca cgcagattat gcagataccg
     3061 ctgattccaa tttaaaatat ttaagattag agggatttaa ttttaatctg aaagaggaaa
     3121 tagaatttag ttcttaccta atgtctattg tcaatatggt ggctaactct atagttgaca
     3181 aacataattt aagctcaaac atacagaaac tcagaatagt tttcaggatt tataaagaat
     3241 aatcctgctt ttggatacaa agaagtaaaa gcagttgctt tgaagtttcg ccggggattc
     3301 catttaattt cataagcagt aattttatct tcatagacct ccagatagtc aatttcggat
     3361 ttgtggatat tacgccaaaa atatgacctg cgcagaatcc cggcattaga aagcagcttc
     3421 attctttcag aaacaatata attctcccac aatttaccta tatcatctct aactgcaatg
     3481 tctctaaaat cgtttattaa gcaatttcgc acaccattgt cccaaaaata gattttacga
     3541 gaacgcttaa tttcattacg ctgatttgta gagtaatttg gtaaacggaa aatgatatat
     3601 gcctgctcta aaagctgaat atacttttga attgtttttt gatccgtaga aattaattga
     3661 gccagttcat tataggagac ctctgaacca atttgcaaag ctaaagcgtt aagaagacgg
     3721 tctaaaagtt ccggtttacg gaggtcttga aaagtgaaaa tatctttata taagtagctg
     3781 cctaccagat gattaagcag agtagcagct tcggagggat gagtaacaat atcgggataa
     3841 gaaccataaa ttaagcggtt agctaattct ttcctttcct gatattctcc aaaattatct
     3901 gcaagttcct gataagcaaa tggatataag taaaattcat attttctgcc ggtgagtggt
     3961 tccattattt ggttggataa ctcaaaggaa gatgagccgg tagcaataat ctgcacttca
     4021 ggaaaattat ccactaagag ttttaacttt aagccaatat tctgaaccct ttgagcttca
     4081 tctataagga tatatttgct tttcccggcg aattgtttca ttgagcttat tgtctctgca
     4141 gccagtagat tacggtcttc caaatcatca caattcaaat ataaaacatc tggctgagct
     4201 gggaaaagac cttttaatac cgttgtcttt ccactctgcc ttgctcccat aattaaaatt
     4261 gcttttcctt tatgcagatt atttaatatg cgagaagtaa taaaccgttt aatcataaga
     4321 agttcctttt cttgatacaa tatatagata agataatcaa taagagaatg ccgtcaagta
     4381 aaataacccc tattttgcag agtatagtcc ggaaaataac ccctattttg cagaatataa
     4441 tctggaaaat aggggttagc atcttgggca ggtaaccttc actggcaaat gttaaattac
     4501 aagtatctaa cttcttgttt ttcaaatggt tgtttaacga ccctctaaca ctactctaac
     4561 actaccctaa cacttgcttt aactctgtta aagaatagat agaaaataga taaatagtca
     4621 tttaagtaaa actatgcagg gaattgaaga atgccctatt aagagtaaat tttataagag
     4681 agtttggata actaaatcta aattaaattt gttttttcaa acacccccct gagagctacc
     4741 caattgtggc ttttgtacag cccggaaagg aaagataata gacagtcggg ttttaagttt
     4801 ccgattgaca ataaagagga agtaaattat tatactttca atatgataag agaggtaaag
     4861 aaaatgaaac gcctttatat aattatcgta atcctcatta tatcaatatg cctttgggct
     4921 caaccctttg agctggataa caatgttttt aatcccagcg gggttcccag tttttcattc
     4981 tcccagccac gctttatgga tatagatggt gacggagatt atgatttttg gttagggaat
     5041 acaaatcggg ctccgcttta tatacagaat aatggaagtg ctaccaaccc tgctttttca
     5101 cctggtcttg atcttttgtc ctcaatttct tatcttgccg cagaagtttc tgtctctgca
     5161 gatatgaatg gggatggaat tttagatttg gttactggtg gttttacagg tcttcattta
     5221 ttcttaaata gcggaactaa tgccagcccc gtttttactg aaagcggggg ttatttttcc
     5281 ggattgaata ctgttatgaa tcctgttccc gatgtagccg atgttgataa cgacggcgat
     5341 ttggatctgg tagtgggttt cagtgaagat ggaagagtaa atgtatattt taatagcggt
     5401 acagctgttt ccggacaatt tagtgaggat aactgtctct tacttgccga tataggtctg
     5461 tatgcttacc ccattttttg cgattttgat ctggatggca aacaggatat attttgcggt
     5521 cgtgatagtt atggttttgt ttattttcaa aataccggta cagctactaa tcctgtctgg
     5581 gaggaaaata atgctctttt tgcaggttta ggaatgtcca cttactggaa ttcacccgat
     5641 ttagttgact taaatggtga tggacaattg gatctggtat atggaactgc cgatggacct
     5701 ttgcagtatt atgtgaataa tggaacggca caaaaccctt cttggcaaga aaatacatcc
     5761 ctttttggag gtgttttgga tgtggatgga gcaagtaatc ctgtttttta tgattttgac
     5821 ggggatgggg attatgacat aattagtggc agtcaaatgg gcgatattaa attttatcgt
     5881 aatacaggaa ctccctattc acctgcctgg caagcagata acgctttttt tgcttccatt
     5941 gatcactcca tatattctgc cgtggcagtt ggcgatgtag atgctgatgg ctttccggat
     6001 gtaattgttg gagatttaaa tgggggattg tattttcatc ataatacggg aacaggctta
     6061 attgaacaga ccggtatttt acctgctgtt tcagttgggg gatggtcttg cccgcgttta
     6121 gtggatatgg attttgatgg agaccttgat ttagtggttg gtaatgaagc aggcaatata
     6181 ttttattatc agaataatgg aacacctgta agtccttcat ggcaagttgt aaatggcttt
     6241 tttggaacaa tagatgtcgg ttccgattgt gttcccactt ttggagattt agatgctgat
     6301 ggtgatttgg atatggttac tggaaatctc tttggtgagg ttcaatgctt ccttcggcaa
     6361 cgccaaattt gggaggaaaa taatgttctc tttaatggaa ttgaaacaga ccaaaatgct
     6421 accccagcat tagtggattt ggatcacgac ggtgatttgg atttagtttt gggcgattat
     6481 gacggcactt ttaagttttt ccggaattta aaatattctg gagcagtttt aaatcctccc
     6541 ttaaatccaa gcgcagaaat taataatgaa attatggttt tgtgggataa tcctactaca
     6601 ggtagcactt ctccctttga acattataat atctatctgg atgaagtttt ctgcggttcc
     6661 accaatgata atttctggat attttccggt ttggaaacca atgtaactta tctggtaagt
     6721 attactgcac aatatattgc gggggaatca atgcctgtta ctatggaagt gatcatcctt
     6781 ggaaatgaag atcctgtcct taaaccttat actttaacca atttccccaa tccctttaat
     6841 cctagtactc agattcaatt ttctctgagc aaagcagaag atgtctcttt gactatccat
     6901 aacataaaag gccaattagt taaaactatg tatctgggaa aagccgaatc tggaatgcat
     6961 accttaactt ggaatggaaa agatgaaagc ggtaatcttt gtgccggtgg ggtttatttc
     7021 tataaactgc aaactaagga tagtgtttta gtgaagaaaa tgctgatgct taaatagcaa
     7081 gaggcctgaa ggtcttgggg tcttaggtct gagaggagaa aagataatag gccagggatg
     7141 aacaggattt tagggattat agggattaga ttttagaaac aaggatttta ggattatagg
     7201 gttgaattat agaaacaagg att
//
LOCUS       DAOSTH010000079         7111 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_9830_length_7111_cov_16.029053, whole genome shotgun sequence.
ACCESSION   DAOSTH010000079 DAOSTH010000000
VERSION     DAOSTH010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7111
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9830_length_7111_cov_16.029053"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..741)
                     /gene="rpoD"
                     /locus_tag="PLB65_07230"
                     /pseudo
     CDS             complement(1..741)
                     /gene="rpoD"
                     /locus_tag="PLB65_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
     gene            complement(1408..1740)
                     /locus_tag="PLB65_07235"
                     /pseudo
     CDS             complement(1408..1740)
                     /locus_tag="PLB65_07235"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
     gene            complement(1762..3519)
                     /gene="dnaG"
                     /locus_tag="PLB65_07240"
     CDS             complement(1762..3519)
                     /gene="dnaG"
                     /locus_tag="PLB65_07240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOQ78356.1"
                     /translation="MPFFPEKEIYTMDQNLIDQIRRANDIVDVIQGYIPLKKVGSNYR
                     GLCPFHNDTRPSLYVNQPKQIYKCFACGKAGNVISFVVDFEKLSFIEAVKKLAARAGI
                     SVPEYEKTKVVNTKREKLLSVYKCAADFFNESLFAYGQEVLDYLRKRSFSPDTAKELH
                     LGYALNSDKALLNHLLKEGYSVSLLKDSGLFGNYGGGLTDLFKDRLIFPIHNSLGEVI
                     AFGGRLLESKPGIGKYINSPGTELYTKGKELYGLFKTKYNISKAGTAIICEGYFDFLR
                     LYANGFTNSVASLGTALTEDQINLLARFCNRIIMLYDGDVAGIKAAIRGGLLCLSKGL
                     EVLIANLPEQEDPDSLILKQGNMAMQEVLGTAIPLISFLATDKRLEQPTAERIELILD
                     ALRMLNDQIKKELLLKEVSEAFGITIGALNSKLRSRGTYRYPASEPVKTAPQNENYEE
                     RNVLILALKDYDSYKLLAKELDSSYFNNKLYRELYKFLIDKNIKEEPWEPESLLDNIE
                     NNDIKESLAELLFEDLQQMRFEDCLHGLRIRKIQHELEEMDRAISKNPNNLELLKEKE
                     KLALKYRRMTKKVVNKVLY"
     gene            complement(3621..5963)
                     /locus_tag="PLB65_07245"
     CDS             complement(3621..5963)
                     /locus_tag="PLB65_07245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HOQ78357.1"
                     /translation="MFYNNPDLEFDTLVAQISKRCNSDLGRAQSANLRPLTDLSAIKK
                     SQALVAEIQEQLARGVNLDFSGLTNLAPLFEDSRNALFAFEEFTAVYNNNIIVEEILD
                     PQDIWEDFPEIAIYLKKLTSFADLNVRYLQIFDTDGEILDGASKELYTIRKRINSLRM
                     QIQKTMQSLLNDNNLERYLQDKYVTQREGRYVLPIKEGSAPFVNGIVQSQSGSKSTVF
                     IEPMAIIPLNNELQLLKQQEKQEVFKIFTEYTAAIKAEKKRILRNQEILAFLDFRFAC
                     GRLCNTIKAKTPVMLSEPCLKLKTARHPLLILRLGSSAKVIPFDLELGEEKKIIILSG
                     PNTGGKTVLLKATGLITLMALSGLPVPADEDSEIGFFENIFADIGDDQSIENALSTFS
                     AHLEKIGKMLQYANPQTLILIDEIGAATDPQQGSALAQAILERFLALNCPTIVTTHYT
                     ALKIFAEQHPKCLNASMQFDTKNLQPTYKFIAGFPGDSFAIEVAASLGIEPKLIDRAK
                     ELAGSQNVQFTELLKKMQEEKKKFSVSSYQNELKTRLLENKLQELAKKEEDWEKEVIQ
                     RRQNYLKELQQELISQQKIYQNELEELKTLSKQERKSLSEYKLHYIETKTAEIQKELN
                     DNINDNLSPIRNPQPGTKVWLSNFDTEAVILSIEGEQAKVDMNGITFQTPVSTLFETK
                     TKEVVIKNETKVKAYIAPKAKFELKILGLTFDEAKPLIDDFLDDATLAGLHSLRIVHG
                     KGTGALRTKVRDYLKKKKQVISIGTPGQAEGGSGVTIVNI"
     gene            complement(6131..6673)
                     /locus_tag="PLB65_07250"
     CDS             complement(6131..6673)
                     /locus_tag="PLB65_07250"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOQ78358.1"
                     /translation="MGVIDWIILAVLLIFTFFGWRKGLLANVVQLCGYILTFFLVSHY
                     YPLVQRSLILKYHFAKWLATIVSVILIVALIVVIARLVIYILEKFLHALKLTSLNRGL
                     GAVLGVVNGLLVVIILMVVLDFLPNISTSLKDGEKHRVYAGVNLVKEEIFTKLNLTER
                     MKLIKLPKLPFLEENEAEDK"
     gene            complement(6766..6990)
                     /gene="rpsU"
                     /locus_tag="PLB65_07255"
     CDS             complement(6766..6990)
                     /gene="rpsU"
                     /locus_tag="PLB65_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOQ78359.1"
                     /translation="MKVVIYLPTVVAKENESFDILLKRFKKKCEKAAILSEIKKNQAY
                     EKPSVRRKREANTARRKMLKMQRRMQRYMK"
BASE COUNT         1969 a         1426 c         1317 g         2399 t
ORIGIN      
        1 ttatttcaca tggaagttat ccagaaactg ttgcaaaata gctgcacgcg aaggatggcg
       61 caatttttta agggcttttt cttcaatttg gcgaatacgt tccctagtca cttgaaaaat
      121 attgccaact tcttccaggg ttctatgata gccatcatca atgccaaagc gcaggcggat
      181 aactctttcc tcgcgtggcg ttagggtctt tagaacttca tctacctgtt ttttcagtag
      241 ggagcgttca gccaggcgtt cgggagaaat aatggttcta tcttcgataa aatcgcccag
      301 gatgctatct tcgctatcgt ggccgatggg tttatccagc gagacgggtt ctttgctgat
      361 cgacataatg tttttaattt tttccacggg gatatccaga acttcggaaa tttcttccgg
      421 atagggatcg cgtcctaatt tttgcaccag gcgacggctg gttctattaa ttttattgat
      481 ggattcaatc atatggactg ggatgcgaat agttcttgct tggtctgcaa tagctcttgt
      541 gattgcttgg cgaatccacc aagtagcgta agtgctgaat ttatagcctt tgcggtaatc
      601 gtatttttcc acggcgcgca taagtcctgt atttccttcc tgaatgaggt ccagaaattc
      661 cagtccacgg ttgttgtagt gtttagcgat actaataacg aggcggacat ttgcctcaat
      721 catttcgttt tgggcttttt ctttattgcg ttcggcgcgg tcaatttcgc gcagcaggaa
      781 aattagttca tttccggtca ttttggtttc cagttctacg cggcggattt ttcggcgggc
      841 gtttttaatt ttgcgtaggg tttctacaaa gacattaggg tcaattcctg tttcatccag
      901 cacttcctga aagtcctcat cgcttttttt ggcgcggcgt ccatacatac aaatttcgtc
      961 tatagtaaag cgtttgatgc gggttaagtt attgatactt tgatagcttt cttcgatgcg
     1021 ttccaccaga gagcggatgc ggtaaaccat tcgtttaatc agtttatcgt tataggatag
     1081 ttgcataaag gtattaacga tttgattgcg aatttcgtca atttcttctt ggcgactggc
     1141 agtgccggta tcatcaaaat cgcaattatc aaggatatat cctacttttt cgaatttttc
     1201 ttcgttttct ttaagaattt ctttgaattt atcgataatt ttggcgtcct gatttttatc
     1261 tatccaggtg ctgatatcca ctttaaaaat ttggtctaag cggactcttc tttcgcggta
     1321 gcgatggagg aagttataca tttcgcgcag cgtgcttccg catttgaaga cattctggtt
     1381 aatcattttt tgataggtct ctatcttttt ggccacgcgc acttcccctt ctctatccag
     1441 caagggaacg cgtcccattt ctcgtaaata catacggaca ggatcgtcat agtagcgttt
     1501 acttttaaat tttttagtag ttgaaggctt acgaatagcc gcaccacttt ttgtaatacg
     1561 tttttccgtt tcgtcgatga tttcgatgcc atcttgggac aaatcgaaga taatatcatc
     1621 aactttatcg aggaagaaag ggcttggggg tagaatatca ttaatctgct taaaagtaat
     1681 gtaaccggat tctttgccca attccaccaa ttttttcaaa cattcgttgt aagtaatcat
     1741 caatgctcct tactgcgtcc ttcaatacag gactttgtta acgacctttt tggtcatacg
     1801 cctatacttt agagcaagct tttccttctc tttgaggagt tccaggttat tagggttttt
     1861 gctaatagcg cggtccattt cttccaattc gtgttggatt tttcggatgc gtaagccgtg
     1921 taggcaatct tcaaaccgca tttgctgtaa atcctcaaaa agaagttcag ctaagctttc
     1981 ctttatatca ttattttcaa tattatccag cagggattca ggttcccagg gttcttcttt
     2041 tatgttttta tctatcagaa atttatatag ttcacgatag agtttattat taaaataact
     2101 cgaatccagt tctttggcaa gtaatttata tgaatcataa tcttttaacg ccaaaataag
     2161 gacatttcgt tcttcatagt tctcattctg aggcgctgtt ttcactggtt cagaggcagg
     2221 ataacgataa gtaccacggc tgcgtaattt actgtttaat gcaccaatag ttattccaaa
     2281 ggcctctgaa acctctttta gcagcaattc ctttttaatt tggtcgttca gcattcgtaa
     2341 agcatctaaa ataagttcaa ttctttccgc agttggttgt tccagacgct tatccgttgc
     2401 cagaaaactg attaacggaa ttgctgtacc taaaacctct tgcattgcca tattgccctg
     2461 ttttaaaata agagaatcgg gatcttcctg ttcaggaaga ttggcgatta gaacttccag
     2521 tcctttgctt aaacataata gaccacctct tattgctgct ttaattcctg caacatcacc
     2581 atcatataac ataataatcc gattacagaa acgtgccaat aaatttattt gatcttcggt
     2641 taaggcagtt cctaaactag caacggaatt agtaaaaccg tttgcataga gccttaaaaa
     2701 atcaaaatac ccttcacaga tgatagctgt gcccgccttg ctgatgttat atttggtttt
     2761 aaacagtccg tagagttctt tacctttagt gtaaagttca gtgccggggg aatttatata
     2821 ttttccgatt ccgggtttgg attccagaag gcgtcccccg aaagcgataa cttcgcctaa
     2881 gctattatga ataggaaaga tcaagcggtc tttaaaaaga tctgttaaac ctccaccata
     2941 atttccgaaa agtccagaat cctttaacag cgaaacacta tagccttctt tcagcaggtg
     3001 atttaaaagg gctttatcgc tgtttaaagc ataaccaaga tgaagctctt tagccgtatc
     3061 tggagaaaaa gaacgctttc ttaaatagtc caaaacttcc tgtccgtaag caaaaaggga
     3121 ttcattaaag aaatccgcag cgcatttata aacacttaag agcttttcac gctttgtgtt
     3181 taccaccttc gttttttcat attcaggaac actgattccg gcacgcgcag ccagtttttt
     3241 aacagcttca ataaaactga gcttttcaaa atcaactaca aaactgatta cattgccggc
     3301 ttttccacaa gcaaaacatt tgtatatttg cttgggttgg tttacatata gcgaaggtct
     3361 ggtatcatta tgaaaagggc ataaaccccg atagttactc cctacctttt tcaagggtat
     3421 ataaccttga ataacatcta caatgtcatt agcgcgacga atttgatcga ttaagttttg
     3481 gtccatagta tatatctctt tttccgggaa gaacggcatt tcgtcgttct cctaacaacc
     3541 aagttttcaa gtttcctata gctaataggt aactacctaa aatatagtgt tgagggttga
     3601 tgaaaacatc aaccatcctg ttatatattc acgattgtaa ctccgcttcc accttcagct
     3661 tggcctggag ttccaatgct aattacctgt ttcttctttt ttagatagtc ccttactttt
     3721 gttcttaagg caccggttcc ttttccgtga acaattctta aagaatgcaa tccagccaaa
     3781 gtagcgtcgt ctaaaaaatc atcaattaag ggttttgctt catcaaaagt aagccccagg
     3841 attttcagtt cgaattttgc cttaggagct atataggctt tcactttcgt ttcgtttttg
     3901 ataacaacct ctttggtttt tgtttcaaaa agggtgctaa ctggtgtttg aaaagtaatt
     3961 ccattcatat ccacttttgc ttgttcaccc tcaattgaca aaataactgc ttccgtatca
     4021 aaattggaaa gccatacttt cgttccgggt tgggggtttc tgatagggct cagattatcg
     4081 ttaatattat cgttaagctc tttttgaatc tcagctgttt tcgtctcaat ataatgcaat
     4141 ttatattccg aaagagattt acgttcctgt ttagacagag ttttcagctc ttccagttca
     4201 ttttggtaaa ttttctgctg ggaaataagt tcttgctgca gttctttcag gtaattttgc
     4261 cgacgctgta taacttcttt ttcccaatct tcttcttttt tggctagttc ttgcagttta
     4321 ttttctagca gacgggtttt tagttcattt tggtagctgg aaacgctaaa tttctttttt
     4381 tcttcctgca tttttttcag caattccgta aattgcacat tttggctgcc ggcaagttct
     4441 ttggcgcgat caattaattt tggctcaatt ccaagggagg cagcaacttc aattgcaaag
     4501 ctatcacctg gaaaacctgc aataaattta taggtgggtt gcagattttt ggtatcaaac
     4561 tgcattgatg cattcaaaca tttaggatgc tgttctgcaa atattttcag agctgtgtaa
     4621 tgcgtagtaa caattgttgg acaatttagc gctaaaaatc tttccaaaat tgcctgagct
     4681 aaggctgaac cctgttgtgg atcggttgca gctccaattt catcaatcaa aattaaggtt
     4741 tgaggattag catactgaag cattttgccg attttttcca aatgtgccga aaaagtagaa
     4801 agcgcatttt caattgattg atcatcacca atatcggcaa aaatattttc aaaaaatcca
     4861 atttcgctat cctcatcagc aggaacagga agccccgata aagccattaa agtaatcaat
     4921 cctgttgcct ttagcaggac tgtttttcct ccagtattag gtccactgag aataattatt
     4981 tttttctctt cgccaagttc cagatcaaaa ggaatcactt tggcagaact gcctaaacgc
     5041 aaaattagta aaggatgcct tgctgttttt agttttaaac aaggctcaga aagcataaca
     5101 ggagtttttg cttttatagt attgcaaagg cgtccacagg caaaacgaaa gtccaaaaaa
     5161 gccaatattt cctgattgcg caaaatcctt ttcttttcag cttttatagc ggcagtgtat
     5221 tcagtgaaaa tcttaaatac ttcctgtttt tcctgttgtt tcaatagttg aagttcatta
     5281 ttcagcggaa taattgccat cggctctata aaaacagtag atttacttcc ggattggctt
     5341 tgcacgatac cgtttacaaa aggtgccgaa ccttctttaa tcggtaaaac ataacggcct
     5401 tccctttgcg ttacatattt atcctgcaga tagcgttcta agttattgtc gtttaagagg
     5461 gactgcattg ttttctgaat ctgcattctt aaggaattta tccgtttacg gattgtatat
     5521 agttccttgg atgcgccatc taaaatctcg ccatctgtat cgaatatttg cagatagcga
     5581 acattgagat cagcgaagga ggttaatttc tttaagtata ttgcaatttc gggaaagtcc
     5641 tcccaaatat cctgagggtc aagaatctct tccacaatta tgttattatt ataaacggca
     5701 gtaaattcct caaaagcaaa cagggcattt cggctgtctt caaaaagagg ggcaagattt
     5761 gttaaaccgc taaaatcgag atttacacca cgagcaagtt gttcttgaat ttcagcaacc
     5821 aatgcctggg attttttgat tgcggataaa tctgtaagcg gccgtaaatt tgctgattgt
     5881 gctcttccta agtcactgtt acagcgtttg ctgatctgcg ctactaaggt atcaaattct
     5941 aaatcgggat tattgtaaaa catacgctat aaagggagac aaaatatctg tgtgatgggc
     6001 attagaaatc cattatacct gtataagcta ttggcaaatg gataattaag aggagcacaa
     6061 gtaactcctg tcttacaaat gcagctaaaa ttattggcgg gtttcaattt cttcaccttt
     6121 ccactttttt ttacttatct tctgcttcat tctcctcaag aaaagggagc ttgggcagtt
     6181 taatcagttt cattctttct gttaaattca gcttagtaaa aatctcttcc ttaactagat
     6241 ttactccagc ataaacccta tgcttttctc catcttttag cgaggttgaa atattgggta
     6301 gaaaatccag cactaccatt aaaataataa caactagtaa accgtttact actcctaaaa
     6361 cagcacctaa gccacgattc aaagatgtca gctttaaagc atgtaaaaat ttctctaaga
     6421 tgtaaataac aaggcgagca atgacaacaa tcaatgccac aattaaaatc acggaaacaa
     6481 tggttgccag ccatttggca aagtgatatt ttaaaattaa actacgctgt accagaggat
     6541 aataatgact cacaagaaaa aaagtgagaa tatatccgca caattgaact acattggcca
     6601 gcaaaccttt tcgccatccg aaaaatgtga atattaacag aacggcaaga atgatccaat
     6661 caataactcc cattcttttc tccgcttaaa aaagcataca gggaatctaa gtccctgtag
     6721 ctaagttctt gattttttta attgtgttct ttggttaata taaccttatt tcatataacg
     6781 ctgcatccga cgctgcatct ttaacatttt gcgccgggct gtattggcct cgcgttttct
     6841 acgaacgctg ggcttttcgt atgcttgatt cttttttatc tctgaaagaa tagctgcctt
     6901 ctcgcatttt ttcttaaagc gttttaataa gatatcgaag ctttcatttt ccttcgctac
     6961 gactgtaggc aaataaatca ccaccttcat ttagtaaatt agtttatacc attattttag
     7021 aatgggtttg gctgtcaaga gagattattg gtctcacgct gattacgcag atttattttt
     7081 tagccaccga aggcaccgaa gccaccgaaa a
//
LOCUS       DAOSTH010000080         6918 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_10139_length_6918_cov_14.352907, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000080 DAOSTH010000000
VERSION     DAOSTH010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6918
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10139_length_6918_cov_14.352907"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            89..1123
                     /gene="mtnA"
                     /locus_tag="PLB65_07260"
     CDS             89..1123
                     /gene="mtnA"
                     /locus_tag="PLB65_07260"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HOQ78360.1"
                     /translation="MQINGQQYLSVWYENAKVKMIDQNKLPLEFRIVEYSNYLEICSA
                     IREMTVRGAPAIGAAGAYGMALAAQNAPDEKFRAYLRTARDELTSTRPTAIDLCNGVN
                     YVYENALKFIPDYEHSRLVAVFAAREFAKKSVDDCYQISKIGSEIVPKGARILTHCNA
                     GALATVDWGTALGVIRMAHRQHKDIFVYVSETRPRFQGARLTAFELAQEDIPHSIITD
                     NAGGFYFWKKEIDLVITGADRICLNGDIANKIGTYEKAVLAKEHKVPFYVAAPFSTFD
                     LSCKEGSDIPIEFRDEDEIKCINSNYLANPYSPALNPSFDITPAKFITGIITPKGIFK
                     PKDAARQVQV"
     gene            1120..2433
                     /locus_tag="PLB65_07265"
     CDS             1120..2433
                     /locus_tag="PLB65_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOQ78361.1"
                     /translation="MIFDLVLENGTIITMDSQNQILENHCIAIKDGMIQAIFPVDSLT
                     YETAEKIDASNCIIIPGLINMHSHLPMTYFRGLADDLPLNIWLQQYIWPLEAKLINPQ
                     FVYDATLHSAAEMIKNGISTANDMYFCMNSIADACSKVGLRVFISEALIDNEQTGNDG
                     TNAIGKRIIELKEEYKDNPLIDFTLAPHSIYACSENTLKNCAKVAGENDFIVHTHLSE
                     TAEEVENCLKEHKLSPVEYLQKIGLLDVQGIFAHGVWINEKEMDILAEKGNSSIAICT
                     ESNLKLSSGFAPIKKYQEKGINICLGTDGVASNNNLDLLAEMSVTAKLHKALNNDPTL
                     LPAKDAFALVTINAAKALGKEKELGSLEIGKIADLAVVSLQELENNPLYNPYSLLVYA
                     INHNSIRDMIIQGKIVMRNRQLLFVDEQHILEKANYYKKMILKPL"
     gene            2436..2864
                     /gene="smpB"
                     /locus_tag="PLB65_07270"
     CDS             2436..2864
                     /gene="smpB"
                     /locus_tag="PLB65_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOQ78362.1"
                     /translation="MKSIKNRRSQHEYYIIQTLEAGIVLKGTEIKSIRAGKVNFKDSY
                     AKIEDGECWLHNLHISPWEKAAYFNHEAERKRKLLLHKHEIKRLRSKVEEQGMTLIPL
                     ELLINEGGLCKVILALAKGKKEYDKRDAMRKKDLERNQEG"
     gene            2990..4465
                     /locus_tag="PLB65_07275"
     CDS             2990..4465
                     /locus_tag="PLB65_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HOQ78363.1"
                     /translation="MKEQNLQTETHLNQITFFLNGKQITEDVPAGISTLDWLHAKKQM
                     FGTKCSCNEGDCGACTVVIAYPFEGIITYRAINSCLYPAAKLHGKHLITIEGLGTPEN
                     LHPIQQTLLKYHSLQCGYCTPGFVMSLFALLAMNSHPDQETILAALEGNLCRCGTYNS
                     ILKAAQELSAKFSPSEIVPEWCRNTEQDLYSFNETDTMFFTKTDIPQQVNCYLFPKTM
                     KQLFDFYAEHPDANFIAGGTDIMVQKNIDRKVFPVLIDITNLPELNRLYLRQDGLHIG
                     SAVTYQQLYESGIVKTDYPVLHKLLAQIASQQIRNLGTLGGNIANASPVGDTLPLLLV
                     LDASLWLQSDLEIRQIPFKDFFVSYRKTALNKGEIIKEIIIPPVKSNSYIKFTKTSKS
                     KSVDISSVITAVKIEYNEDIIINAALALGGVSALPMLSQKFTELLTGKKLSTINIDSV
                     ISEVEKEFEPLTDIRGTALYRKKLIQNHILLYFQELQKEEK"
     gene            4462..6753
                     /locus_tag="PLB65_07280"
     CDS             4462..6753
                     /locus_tag="PLB65_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOQ78364.1"
                     /translation="MNSAVFHLSGPLHLCGKSHFIADEPPLQGQLYAKFLFSPVAHAQ
                     ISNLDISAAEKIPGVIKIITADSIPGENQIGTVVKDEPLFPETEIMYVGQPIAMVLAE
                     NEYLAELAVKKIVLKYKELPAIFDPEEAYKEKQGYIPERIIARGDVETALKNAPFTLS
                     GKTETSGQEHFYLESQRCLAVPMENSRIILHTATQSTMEVQEIASRILGIPVNDVIIE
                     VPRLGGAFGGKERGATIWACMAALGAFLTQKPVLVKLTRDEDMHLTGKRHPFTSLWKV
                     GFDEHGKILAYDILLLANGGAYTDLSIAILERAMFHSENAYYIPNIKIKGFACKTNLP
                     PNTAFRGFGAPQGIFVIENILAKIAEQLKIDPLEIRLQNAYQNGDKTPYGQEISDCRL
                     TDLFNILAEKTNYLALKQAVEVFNKNNTRFKRGIGIIPVKFGISFTTTLLNQGSALVW
                     VYIDGSVSVSTGGVEMGQELSTKVAIVVAKVLGIPVHQIRVESSNSQRIGNASPTAAS
                     TGSDINGNAARIAAEKIKMALTECAVKLLKQKHQLNVSPEDIVFKEGKVFYKENPDAC
                     VNFEELVRFAHSERVPLAAYGYYKTPALWFDRDKGSGNPFHYFVWGVALAEVEMDTLL
                     GEHKIIKLTVMHENGQTLNEEIDRGQIEGAIMQGIGWCTMEDLKWNEKGVYLSANPST
                     YKIPGIRNLPEELEIEMIPSATEEASVFGSKGIGEPPLIYGLSVFFALQAAAKEAKKD
                     AELSFPATPESVLQCLDGLYPIG"
BASE COUNT         2240 a         1271 c         1417 g         1990 t
ORIGIN      
        1 aaaaatgcta agaaagatat tgacgaaatt gccacttgtc agagaaaata cttcttaacc
       61 aaaaagatag ttaagttatg gagaataaat gcaaataaac ggacaacaat acctttccgt
      121 ttggtatgaa aatgccaagg taaaaatgat agaccagaat aaattaccgt tggaattccg
      181 gattgtggaa tacagtaatt atctggaaat atgttctgcc atcagagaaa tgacagtgcg
      241 cggagctcct gccattggag ctgccggagc ttatggaatg gctttggcgg ctcaaaatgc
      301 tcccgatgaa aaatttcggg cttatttaag aacagcaaga gatgaattaa cttccactcg
      361 ccctactgct attgacctct gtaatggtgt aaattatgtt tatgaaaatg cacttaagtt
      421 cattccggat tatgaacatt cgcgcttagt tgcagttttt gcagcacggg aatttgctaa
      481 gaaaagtgtt gatgattgct atcagataag taaaataggc agtgaaatag ttcctaaagg
      541 tgcccgaatt ttaactcatt gtaatgcagg tgccttagct actgtagatt ggggaactgc
      601 tttgggggta attagaatgg ctcatcggca acataaagat atttttgttt atgtaagtga
      661 aacgcgtccc cgttttcaag gagctcgttt aactgccttt gagcttgccc aagaagatat
      721 tcctcattca ataataacag ataacgcagg tggtttttat ttctggaaaa aggaaattga
      781 cttggtgatt accggtgcgg atagaatctg tctgaatggc gacattgcca ataaaatagg
      841 cacttacgaa aaagcagttt tggcaaaaga gcacaaagtg ccattttatg tagctgcgcc
      901 ttttagtact tttgatttat cttgtaaaga gggctcggat attccgattg aatttcgtga
      961 tgaagatgaa ataaaatgta taaacagcaa ctatcttgcc aatccttatt caccagcatt
     1021 aaatccttct tttgatataa ctccggcaaa atttataacc ggaatcatca ctcccaaagg
     1081 tattttcaaa ccaaaagatg cagccaggca ggttcaggta tgatttttga cctcgttttg
     1141 gaaaatggca ccatcattac aatggatagc caaaaccaga ttctggaaaa tcattgtata
     1201 gcgataaaag atggaatgat ccaggcaatt ttccctgttg acagcttaac ctatgaaact
     1261 gcggaaaaaa tagatgccag taactgtata attattccgg gattaataaa tatgcattca
     1321 catctgccga tgacttattt tcgcggtctg gctgatgacc tacccttaaa tatttggctg
     1381 caacaatata tctggccatt ggaagcaaaa ctgattaacc cgcaatttgt ttatgatgct
     1441 actttgcata gtgctgcaga aatgattaaa aatggaatca gcactgccaa tgatatgtat
     1501 ttttgtatga attccatagc cgatgcctgc tctaaagtcg gtttgcgtgt ttttattagt
     1561 gaagcattga ttgataatga acaaacgggt aatgacggaa ccaacgctat cggtaaaaga
     1621 attattgaac taaaagagga atataaagat aatccgctga tagattttac tttagctccg
     1681 cattccattt atgcctgcag tgaaaatacc ttaaaaaatt gcgctaaagt tgccggtgaa
     1741 aatgatttta ttgtgcatac ccatttatct gaaactgcgg aagaggtaga aaactgcctt
     1801 aaagaacata aattaagccc agtggaatat ctgcaaaaaa ttggtctctt ggatgttcaa
     1861 ggtatttttg cccatggagt ttggattaac gaaaaagaga tggatatcct tgctgaaaaa
     1921 ggaaatagca gtattgccat ctgcacggaa agcaatttga aattatcctc tggttttgcc
     1981 cctataaaaa agtatcagga aaaaggcata aacatctgtt taggaaccga tggtgtagcc
     2041 agtaacaata atctggacct gctggctgaa atgtccgtaa ctgcgaaact gcataaggca
     2101 ctaaataatg accctactct gttgccagct aaggatgcct ttgctttagt aacaataaat
     2161 gcagctaagg cattgggaaa agaaaaagag ctcggttcac tggaaatagg aaaaattgcc
     2221 gatctggctg ttgtttcttt acaggaattg gaaaacaatc ctctctacaa tccttattct
     2281 cttctggtgt atgctattaa ccataatagc attcgagata tgattattca agggaaaata
     2341 gtgatgcgca atcgccaact gctttttgtg gatgaacagc acatcctgga aaaagccaat
     2401 tactacaaga aaatgatttt gaagccatta taataatgaa atctataaaa aatcgccgct
     2461 ctcaacacga atattatatt atccaaactc tggaagcagg tatagtttta aaaggtacgg
     2521 aaataaaatc catccgtgcc ggaaaagtga attttaaaga tagctatgca aaaattgaag
     2581 acggagaatg ttggttacat aatttgcata taagcccttg ggaaaaagcc gcttacttca
     2641 atcacgaagc tgaacggaaa agaaaattac tgctgcataa acacgaaata aaacgcttgc
     2701 gttctaaagt ggaagaacaa ggtatgactc ttattccctt ggaacttttg atcaacgaag
     2761 gaggactctg taaagtgatt cttgccctcg ccaaaggtaa aaaagaatat gataaacgag
     2821 atgctatgcg gaaaaaagac ctggaaagaa accaggaagg ttgatatccc tctcaaccca
     2881 aaaagtatat ttttacagtt acttataagt aatagggaac ttaataactc gaagaaaaat
     2941 ggaacgatga agacatcgtt ctgcccctct tcccattaaa ggaggcaata tgaaagaaca
     3001 gaatttgcaa acggaaaccc atttaaacca gatcactttt tttcttaacg gcaaacaaat
     3061 tactgaagat gtccctgccg gaatttccac tttagattgg ttgcacgcaa aaaaacaaat
     3121 gttcgggaca aaatgcagtt gtaatgaagg tgattgtggt gcttgcacag tggtcattgc
     3181 ctatcctttt gaaggcataa ttacttatag ggcaataaat tcctgtcttt atcccgcagc
     3241 caaattacac gggaaacact taattacgat tgaaggttta ggtactccgg aaaatttaca
     3301 tcctatccaa cagacgctgc taaaatatca ttctcttcaa tgcggatatt gtacccccgg
     3361 ttttgtgatg agtttgtttg ctcttttagc tatgaattcg catcccgatc aggaaacaat
     3421 tttggctgct ttggaaggaa acctatgtag atgtggaact tataatagta ttttaaaagc
     3481 agcacaagaa cttagcgcta aattttcccc ttcggaaatt gttcctgaat ggtgtagaaa
     3541 cacagaacag gatttatata gctttaacga gacagatact atgttcttta ctaaaacaga
     3601 tatcccacaa caggttaatt gctatctctt tcctaaaact atgaagcagc tttttgattt
     3661 ttatgctgag catcctgatg ctaattttat tgctggcgga acagatataa tggtgcaaaa
     3721 aaacatcgac cgcaaggttt ttccggtgct gatagatata actaacctac cggaactaaa
     3781 ccgtttatat cttcgccagg atggattgca tattggctct gcagttactt accaacaact
     3841 ttatgaatcc ggtattgtaa agacagatta tccggttttg cataaactgc ttgctcagat
     3901 tgcttctcag caaatacgca atttgggaac tttgggtgga aatattgcca atgcttctcc
     3961 cgtgggtgat acacttcccc ttttattggt tttagatgct tccctctggt tgcaatcaga
     4021 tttggaaatt aggcagatac ctttcaaaga tttctttgtt agttaccgta aaacggctct
     4081 aaacaaggga gaaataataa aagaaattat tattccacca gttaaaagca acagctatat
     4141 aaagttcact aaaacttcca agagcaaaag tgtggatata tcttctgtaa ttactgcagt
     4201 taaaattgag tataacgaag atattattat aaatgctgct ttagctttgg gaggggtttc
     4261 cgctctgcca atgcttagcc aaaaatttac agaactacta accggtaaaa aacttagcac
     4321 cataaatata gattccgtta ttagcgaagt ggaaaaagaa tttgagccct taacagatat
     4381 acgcggaact gctctctacc gtaaaaaact aatccaaaat catattttgc tttattttca
     4441 ggaactgcaa aaggaggaaa aatgaattct gctgtttttc atctttctgg tcccttgcat
     4501 ttatgtggaa aatcacattt tattgctgat gaaccacctt tacagggtca actttatgcc
     4561 aaatttttat tttcgccggt agcgcatgct caaatttcta acttggatat ttctgcagcg
     4621 gaaaaaatac ccggtgtaat aaaaattatc acagctgatt ccatccccgg tgaaaatcaa
     4681 attggaacgg tagtaaaaga cgagcccctc tttcctgaga cggaaattat gtatgttggc
     4741 caacccatag ctatggtgct tgcggaaaat gaatacctgg cagaactggc agttaaaaag
     4801 atagtgctaa aatataagga attacccgct atttttgatc ccgaagaggc atataaagaa
     4861 aaacagggat atattccaga acgtataatt gccagaggtg atgtagaaac tgccttaaaa
     4921 aatgcccctt tcactttatc ggggaaaacc gaaaccagtg gccaggaaca tttttatttg
     4981 gaaagccaac gctgtcttgc agttccaatg gaaaacagca gaataatttt acatactgcc
     5041 actcaaagca ctatggaagt tcaagaaatt gcttcccgca ttttagggat tccggtaaat
     5101 gatgtaatta tagaagttcc gcgtttggga ggtgcctttg gtggaaaaga acgcggagct
     5161 acaatttggg cttgtatggc tgctttagga gcttttttaa cacaaaaacc ggttctggta
     5221 aaacttacgc gtgatgagga tatgcaccta accggtaaaa gacatccctt cacttctctg
     5281 tggaaagtgg gttttgatga acacggcaaa attctggctt acgatatatt gcttttagcc
     5341 aacggaggtg cctacacaga tctatccatc gctattttgg aaagagcgat gtttcatagt
     5401 gaaaatgctt actatatacc caatatcaaa ataaaagggt ttgcctgtaa aacaaatctg
     5461 ccgcccaata cggcttttag aggttttgga gctcctcagg gaatcttcgt aatagaaaat
     5521 atcttagcta aaatagcgga acagttaaaa atagacccgc tggaaattcg cctccaaaat
     5581 gcttatcaaa atggagataa aactccttac ggacaggaaa tatccgattg ccgtttaact
     5641 gatcttttta atattttggc agaaaaaaca aactacttag cgctgaaaca agccgtggaa
     5701 gttttcaata aaaacaacac ccgctttaaa cgcggcattg gcatcattcc tgtaaaattt
     5761 ggtatttctt tcacaacgac tttgctaaat cagggctcgg ctttggtttg ggtatatatt
     5821 gatggcagcg tttccgtttc taccggggga gtggaaatgg gacaggaact ttctactaaa
     5881 gtggcaattg tggttgctaa agtattagga attccagttc atcaaattag ggtggaaagc
     5941 agtaacagtc aaagaattgg caatgcgtct cctacggctg cttctactgg aagtgacatt
     6001 aatggtaacg ctgcccggat tgcggctgaa aaaataaaaa tggctttgac tgagtgtgcg
     6061 gttaagctac tgaaacaaaa acatcagctg aatgtttctc ccgaggatat tgtttttaag
     6121 gaaggcaagg tcttctacaa agaaaatccc gacgcctgcg tgaactttga ggaattagtc
     6181 cgttttgctc actcggaaag agttcccttg gcagcttatg gctattataa gacacccgct
     6241 ttgtggtttg atagggataa aggcagcggc aatccttttc actattttgt gtggggagtt
     6301 gccttagcgg aagtggaaat ggatacactt ttgggcgaac ataagatcat caaacttacg
     6361 gttatgcacg aaaacggaca aactttgaat gaggaaatag accgcggaca aattgaaggt
     6421 gccataatgc aaggcattgg ttggtgcaca atggaagatc tgaaatggaa tgaaaaaggg
     6481 gtttacctaa gtgccaatcc ttccacttac aaaattcccg gaattcgcaa tctgccagaa
     6541 gaactggaaa tagaaatgat accctctgct actgaagagg caagtgtttt tggctccaaa
     6601 ggaattgggg aaccaccttt aatttacggg ctttctgtgt tttttgcttt gcaggcagcg
     6661 gcaaaagaag caaaaaaaga tgcggaactg agttttccgg ctactccgga atctgttttg
     6721 caatgcctgg atggtttata cccgataggt taacctcctg gctaacctca acagtgtgtt
     6781 ttttagcatt attgcaaacc agcataaagc aataaacata aaaaacgcat tttaagctct
     6841 ctggtaactt tcttgcagct gccttagtaa atttgtatct tacaatcttg ctttaatcgt
     6901 ggctatatat tccttcca
//
LOCUS       DAOSTH010000081         6823 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_10321_length_6823_cov_11.062352, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000081 DAOSTH010000000
VERSION     DAOSTH010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10321_length_6823_cov_11.062352"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            482..964
                     /locus_tag="PLB65_07285"
     CDS             482..964
                     /locus_tag="PLB65_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HOQ78365.1"
                     /translation="MELITEPLKFPSDCNIILGQSHFIKTVEDLYEALVTSVPNIKFG
                     LAFCESSGPCLIRKEGNDNELIEIAAENMFRLGAGHSFLIILQNAFPINVLPAIKRCQ
                     EVVNIFCATANPVEVILAQSEQGRGILGVIDGFSPKGIELDTDITHRKKFLLDIGYKR
                     "
     gene            969..1547
                     /gene="thpR"
                     /locus_tag="PLB65_07290"
     CDS             969..1547
                     /gene="thpR"
                     /locus_tag="PLB65_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOQ78366.1"
                     /translation="MIRTFIALELPKPLKSRLDSVINRYSKVTPVGVNWVKPENLHLT
                     LLFIGDVQPAQLSEIEEVLSHQLTKIPAFNFTSEGLEFFPATNPHLLWLKLSSETDDI
                     FKLNRCLIRELSTKGIMPDKKAMKLHITLARLKMNLAPELEREIMSKDLKQGPLVFDS
                     ICLYKSLLLPEGPQYTILNKYNLLDSRRLNAG"
     gene            1537..2592
                     /gene="recA"
                     /locus_tag="PLB65_07295"
     CDS             1537..2592
                     /gene="recA"
                     /locus_tag="PLB65_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOQ78367.1"
                     /translation="MLDKNKDAALKTAISQLEKKYGVGTLMRLGDKPNQAVDVIPTGA
                     PNLDIALGIGGIPKGRITEIYGAEASGKTTIALHIAAECQKLGGTVAFIDVEHALDPF
                     YAKRLGVEIDNLLLSQPDGGEQALEITETLVRSSAVDLIIIDSVAALVPKQEIEGDMG
                     DSHVGLQARLMSQALRKLTAIVSKSNTAVIFINQTRMKIGVTPYMNPETTTGGVALKF
                     YSSVRLEVRHIGQIKDVGSDSQVIGTRTRVKVVKNKFAPPFKTVEFPIIFGQGISKLD
                     ILLDMAVQQDIIKKSGSWFSYQDLKLAQGADKTKLYLTENPQLLEEIETKLREKLKPE
                     DFNLTPEENVPEDNEES"
     gene            2564..3199
                     /locus_tag="PLB65_07300"
     CDS             2564..3199
                     /locus_tag="PLB65_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HOQ78368.1"
                     /translation="MYLKITKKAENDKKSLIIIDNQIWGILPDRILLPFFPIPYTGEI
                     NNDQAKELLSLIEKQARQQLLKYLGNREHSSLECKQYLKRKHYPTELVDTLICEFQDK
                     KYIDDHRYVQILISSLISRKKSKNAIAMKLKETRLPTEIWKELLNELYNQEEGMENLK
                     EQITQLRFRYRDLPPSKQKEKIYASLYRKGFNLNDIHLAWQATAQEIILVD"
     gene            3377..>6823
                     /locus_tag="PLB65_07305"
     CDS             3377..>6823
                     /locus_tag="PLB65_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HOQ78369.1"
                     /translation="MKRISYFVLVLLLITGGVLSAQTLVSIGSGTTVNTTTGVPTPYG
                     TYYKNFHQQFLVLASELNDAGGGAGNITSIAFNVDNVNNCSPMPNYTIKLKQTTQTTL
                     STTFEVGDYTTVWSAPEFMPVNGWNVHTFTTPFNWDGASNLIVDIVTTLITGSYTQNA
                     SCYYSTTSFNSSLRYQSDSVDASTSLTGTVSTNRSNIQFVMAPIIVTNPPNPAMLVSP
                     ANGAILINPNVNLNWNSGGGAPSGFKLSLGTNDPPTNILNNQDMGLVTTYNPPQDFDL
                     NTTYYWKVTPYNAIGDAINCPVWSFTTYPAPIVTQLPYMQNFDISVIPPALPYDWTSI
                     VQATVTTAYVNTLASTTYAHSQPNCVRLYNSTDANATVMLVAPVIGDALDPHSVRIKF
                     WARSSSAGYPLSVGVLTNQTDPATYQEVQNIALTTTLTEYVVDLTTYTGSGRYITFKH
                     GLGGTSRTLYLDDISLELIAPNDLAALSVTGNVTPSVGNASNYTVTVKNWGTATQNAY
                     IVKLMSGDTELASVPGQSITPGSTVSVVVTWTPIAEGPMSIYGKVVLTGDVNPVNDNS
                     PVMNISVMPPGATVVTIGEGNLAEGVPWEFFYKSSLFQTLYYQAELGVMGNITAITFY
                     NNFVTNLTDKPVKLWLGTTDLADLSAGWILNGLTLVYDGTLNFPSGENTIVVPLQTPY
                     LYTGGNLVLYANRPMDTQYFSSSDNFRAQTIGTTRARKLMSDSVIYDPMNPSATGTLS
                     GTFPMTSFTFVVTGMGSLSGTVTSSGSPVADVLIQVSSANYNYSRITSATGQYNFEYL
                     PIGTYTVTASKLGYETQTVNVTIIEDQNTIQNFSLVASSTVSVSGHIVGSDQPTVGIA
                     NAHIFLDGPLDYEGTTNANGDFTITGVLSGNTYNYTIQAIGYADFTGTVVVGSTSVNM
                     GTLVMSELALPPVQILAVENTAQTQVTLTWRPPGSTGTGAGLEDFEIDDGGWVSSGFG
                     DWEWGHYNVTAYTDIDTYVDTPPPSAHSGTGMWGTVLEGGYSNCGAWSYLRKTFDFST
                     ISNPVLSFWHYMNGYNTWDYGLIKVNGNTVWGNSSSAVFMPWQELTINLSAYANMNSV
                     EISFEWYATTTVSYAGWYIDDIYVGHSINKTVNYVYKPIPEKEHLLSEEEEALLHSKQ
                     YVQ"
BASE COUNT         2086 a         1386 c         1309 g         2042 t
ORIGIN      
        1 tccggttgtt ctttctaatc ggcagccacc ataacatata gataaaaaca tacaccttgt
       61 gcatgaacta gcaaaaacaa tcttcatttg ttcttccatc ctcttactaa tggaatcaaa
      121 tccatctttt atttttccac ataagcattt ctaaattcct gtgttacttg cataaatata
      181 tgtccataaa actgtcaata ataaaaacat tctttgccca atataaacat agaaacttcc
      241 caaaatactc attccgaata ttataaatcc tcatccatta cttcaaattg acttcacttg
      301 gacaaatcaa cctatcactt tatcaataaa tatgatatgg cattgttagg gaatttgttt
      361 ggacttggat agttgaagtt ttcagtgagg gtctatatct gaaaactttt gcttgacagc
      421 gctattcatt tttatttaat gagttttatt aaaatttaga ggcaggaagg aagttagcgc
      481 catggaacta attacagaac cactcaagtt tccctctgat tgcaatatca tactgggtca
      541 aagccatttt attaagaccg tggaagattt atatgaagca cttgttacca gcgttcccaa
      601 tatcaaattc ggtttggcat tttgtgaatc ctcaggccct tgcttaattc gtaaagaagg
      661 caatgataat gaactgatag aaattgccgc ggaaaatatg tttcgtttag gcgctggcca
      721 cagctttttg attattttgc aaaatgcttt ccctattaat gtcctaccgg caattaaacg
      781 ctgccaggaa gtagttaata ttttttgtgc tacagccaat ccggtggaag taattttagc
      841 tcaatcagaa cagggacggg gaattttagg tgttattgat ggtttttcgc caaagggaat
      901 tgaattggat accgatataa cgcaccgaaa gaaatttcta ttagatattg ggtataaacg
      961 ctaaaataat gatacgcact tttattgctc tcgaattacc gaagcccttg aaaagtaggc
     1021 tggatagcgt tattaaccgt tattctaaag ttactcctgt gggggtaaat tgggtgaagc
     1081 cagaaaatct acatctcact ttgcttttta ttggtgatgt tcaacccgct cagctttctg
     1141 aaattgaaga agtgctttcc caccaattaa ctaagatccc tgctttcaat tttacttccg
     1201 aaggcttgga attctttcca gcaaccaacc cccatctttt gtggctgaaa ctctccagcg
     1261 aaaccgacga tattttcaaa ctaaatagat gcttaattag agagctttct acaaaaggaa
     1321 ttatgccaga taaaaaagca atgaaactgc atatcacctt agcccgttta aaaatgaact
     1381 tagcgccgga actggaaaga gaaataatga gcaaagatct aaaacaaggc cctctcgttt
     1441 ttgacagtat ctgcctgtat aaaagtctgc tgctgcccga agggccacaa tatactattc
     1501 taaataaata taatctattg gacagccgga ggttaaatgc tggataaaaa caaggatgcc
     1561 gctctgaaaa cggcaatttc gcaactggag aaaaaatatg gagtgggaac tcttatgcgc
     1621 ttaggagata aacctaatca ggccgtggat gtaattccca ctggagcacc aaacttggat
     1681 attgctttag gcattggagg aattcccaaa ggtagaataa ctgaaattta cggagctgaa
     1741 gcatcaggta aaacaactat tgctttacat atcgcagcag aatgtcaaaa actgggtgga
     1801 acggttgctt ttatcgatgt ggaacatgct ctcgatcctt tttatgccaa gcgcttaggc
     1861 gtggaaattg ataaccttct tctctcccaa cccgatggtg gtgaacaagc cttagaaatc
     1921 acagaaacct tggttcgcag ttctgctgtt gatttaatca ttattgattc agtggcagct
     1981 ctggttccta aacaagaaat tgaaggagat atgggagata gccatgtagg tttacaggca
     2041 aggttgatga gccaggcttt gcgtaaatta accgctatcg tctccaaaag caataccgct
     2101 gtaattttta tcaatcaaac ccgtatgaaa ataggagtta caccctatat gaatccagaa
     2161 accacaactg gtggtgtagc gctaaaattc tattcttccg tacgtttgga agttcgccat
     2221 ataggtcaga taaaagatgt gggttcagat tctcaagtaa taggaacccg caccagagtt
     2281 aaagtggtta aaaacaaatt cgcccctcct tttaaaactg tggaatttcc tattattttt
     2341 ggtcaaggta tctctaaact cgatattcta ttagatatgg cagtgcagca ggatattatt
     2401 aagaaaagcg gttcctggtt ctcctatcag gacttaaagc ttgcccaagg tgcggataaa
     2461 actaaactgt atctaaccga aaatcctcag ctgttggaag aaattgaaac aaaattgcgg
     2521 gaaaagctga aacctgaaga tttcaaccta actcctgaag aaaatgtacc tgaagataac
     2581 gaagaaagct gaaaacgata aaaaatccct tatcataatt gacaatcaaa tatgggggat
     2641 tcttcctgat agaatcctcc ttcctttctt ccctatccct tatacaggag aaattaacaa
     2701 cgaccaagca aaagaactgc tttccctaat tgaaaaacaa gccagacaac aattactaaa
     2761 atatctaggc aacagagaac atagctctct cgaatgtaaa caatacctta aaagaaaaca
     2821 ttatcccaca gaattggttg atactcttat ttgcgaattc caagataaaa aatatattga
     2881 tgaccaccgc tatgtgcaaa tcctgattag ttcacttatc tcacgcaaga aaagtaaaaa
     2941 tgcaatcgca atgaaattga aagaaactcg cctgccaact gaaatttgga aagaactact
     3001 aaatgaactt tataatcagg aagaaggaat ggaaaatctg aaagaacaga ttactcagct
     3061 gcggtttcgt tatcgggatt taccccccag caaacagaaa gaaaagatat atgcttccct
     3121 ttaccgtaaa ggctttaacc taaatgatat tcatcttgcc tggcaagcta ctgctcaaga
     3181 gattatcctt gtggattaaa ataatgttgc ataaggtata agaattgctt attttatatt
     3241 gagatatata actgtgtgta cggttcctac tccaaattag atgagatgat atagagtgtc
     3301 ttgcaaataa ccggtagtat gcatatttac tccggaagta atgctaacct aacataaccc
     3361 aaataaggag tatactatga aacgaataag ttacttcgtc cttgtgttat tgctgattac
     3421 aggaggtgtt ctttctgccc aaaccctggt cagcattgga agtggaacca cagttaatac
     3481 tacaacggga gttcctacac cttatggaac atattacaag aacttccatc aacagttttt
     3541 agttttggca tccgaactta atgatgctgg tggaggagct ggtaatatca catctattgc
     3601 cttcaatgtc gataatgtaa ataattgtag tccgatgccg aactacacta tcaaattgaa
     3661 acaaacaact caaactaccc tgagcactac ttttgaagta ggagattata caactgtttg
     3721 gagcgcaccg gaatttatgc ctgtaaacgg atggaatgtt catactttta caactccctt
     3781 taactgggat ggtgcctcca acctcatagt agatattgtt accactttaa ttaccggaag
     3841 ttacactcag aatgcctctt gttattattc taccacttct ttcaatagct cgctccgcta
     3901 tcaaagtgat agtgttgacg cttctacatc ccttactgga actgtatcta ctaaccgctc
     3961 caatatacaa tttgttatgg ctcctattat agtaaccaat cctcccaatc cggcaatgtt
     4021 ggtttctcca gccaatggag ccatccttat taatcctaat gtaaatttaa attggaatag
     4081 cggtggcggc gctccctctg gttttaaact ctctttggga accaatgatc ctcccaccaa
     4141 catattgaac aatcaagata tgggcttggt taccacttac aatccccctc aggattttga
     4201 tcttaacact acctactatt ggaaagtaac tccttataac gctataggag atgcaataaa
     4261 ctgccctgtt tggagtttca ctacttaccc tgcaccaatt gtaactcaac ttccctatat
     4321 gcaaaacttc gatatctcag ttattcctcc cgctttacct tatgattgga caagcatcgt
     4381 acaagcaaca gtaactactg cttatgtaaa cacattggct tctacaactt atgctcatag
     4441 ccaaccaaac tgtgtccgtc tttataattc tacggatgcc aatgctacgg ttatgttagt
     4501 agctccagtt attggagatg ctcttgatcc tcattcagtc agaattaaat tttgggctcg
     4561 ttccagtagt gccggctatc ctctttccgt aggggtctta actaatcaaa ccgatccagc
     4621 cacttatcag gaagtgcaaa atatagctct tactacaacg ctaacagaat atgtggttga
     4681 tttgactact tacacgggat ccggacgcta tattactttc aaacatgggc tgggtggaac
     4741 aagccgcact ttgtatctgg atgatatcag cttggaatta attgctccta acgatttggc
     4801 tgccctttct gtaaccggaa atgttactcc cagcgtaggc aacgcctcga actatacagt
     4861 aacagtgaaa aattggggaa ctgccaccca aaatgcctat attgttaaat taatgagtgg
     4921 cgatacagaa ttggcaagcg ttcccggaca atcaattact cctggatcta ctgtttctgt
     4981 agttgtaact tggacgccta ttgcagaagg accaatgagc atttatggaa aagtagtcct
     5041 taccggtgat gtaaatcctg ttaacgataa ttcaccggtt atgaatattt ctgttatgcc
     5101 tcccggagcc actgtagtaa ctattggaga gggtaatctt gctgaaggcg tgccctggga
     5161 atttttctat aaaagcagct tgttccaaac tctgtattac caagctgaac ttggggtgat
     5221 gggaaatatt actgcgatta ctttctacaa taactttgtc accaatttaa ctgataaacc
     5281 agttaaactg tggctcggaa caactgattt ggcggatctt agtgcgggct ggattttgaa
     5341 tggtttaacc ttggtttatg acggcactct gaactttccc agcggagaaa ataccattgt
     5401 agttccgcta caaacacctt atctctatac cggtggtaat cttgttctct atgccaaccg
     5461 ccctatggat acacagtatt ttagcagtag tgataatttc agagctcaga caattggcac
     5521 tacccgtgct cgtaaactta tgtcagattc agtgatttac gatccgatga atccttcggc
     5581 caccggaacc ctttccggaa cttttccaat gacctcattt acttttgtgg taacgggaat
     5641 gggttcttta agcggaactg ttaccagtag cggttctcct gttgccgatg ttttgattca
     5701 agtaagtagt gctaattata attactcccg cattacctct gcaacaggac aatacaattt
     5761 tgagtattta ccgattggaa catataccgt tactgcttct aaacttggtt atgaaaccca
     5821 aacagtgaat gtaaccatta ttgaagacca aaatacaata cagaatttca gcttagtagc
     5881 ttccagtact gtatctgtaa gtgggcatat tgtgggtagc gatcaaccca cggtaggtat
     5941 tgccaatgct catatatttt tggatggacc tctggattat gaaggcacta ccaatgccaa
     6001 tggtgatttt accatcaccg gtgtcctttc ggggaatacc tataactata ctattcaggc
     6061 aattggctat gcagatttta ctggaaccgt ggtagttggc tccacaagtg taaatatggg
     6121 aaccctggtt atgagcgaat tagccctacc cccggtgcaa attttagcag tggaaaatac
     6181 tgctcaaacc caagttacac tcacttggcg tcctcccggc tcaacaggaa ctggagctgg
     6241 tttggaagat ttcgaaattg acgatggcgg ctgggtttcc agtggtttcg gtgactggga
     6301 atggggtcac tataatgtta ctgcttatac agatatagat acttatgtgg atacacctcc
     6361 tccctccgca cattctggaa ccgggatgtg gggaacagtt ttagaaggag gttattctaa
     6421 ttgtggtgca tggtcttatt tgcgcaaaac ttttgacttc tccactattt ccaatcctgt
     6481 cttaagtttt tggcactata tgaacggtta caatacctgg gattatggat taataaaggt
     6541 gaatggcaat acagtttggg gaaatagttc ctctgctgtc tttatgcctt ggcaggaact
     6601 tactataaat ctttcggctt atgctaatat gaatagtgta gagataagtt ttgaatggta
     6661 tgctacaact acggtttcct atgctggatg gtatattgat gatatctatg ttggacattc
     6721 tataaacaaa acagtgaatt atgtatataa gccaataccg gaaaaagaac atttgctttc
     6781 cgaagaagaa gaggccttgt tacattctaa acagtatgtt caa
//
LOCUS       DAOSTH010000082         6670 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_10602_length_6670_cov_10.925170, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000082 DAOSTH010000000
VERSION     DAOSTH010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6670
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10602_length_6670_cov_10.925170"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(537..1535)
                     /locus_tag="PLB65_07310"
     CDS             complement(537..1535)
                     /locus_tag="PLB65_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOQ78370.1"
                     /translation="MSKRIFNSFLRNLRRDILFNYIEGDRYLTVREIASKFNVSLQTA
                     QKGVHALKAEGLILSKQKSGITVLNKHMHGDTLKKDLVVISNKQDERFHRAFFDGVLD
                     RLVGTGVVAQLIINTEADTNSLQFGEYLESLQCDGIIALSFKNAALPFYHAIHKGVNI
                     ISDIIIDELPILPAIQTDNYKHAFQAGKILLQNDYKSFYAFGYYPEANRRYQGFYDAV
                     NLRAKSVNYIHLSDMDALSKADAVFNNFDATTTIFSCDYSANYILASLFLKHDTPIQG
                     YNFLAYDSESDFFHYPGLPPIRCVAPSFYCLGKALADSMILKWQTGAFQQPLQQKI"
     gene            1852..3174
                     /locus_tag="PLB65_07315"
     CDS             1852..3174
                     /locus_tag="PLB65_07315"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOQ78371.1"
                     /translation="MKKVCVFLLVVLLLLPALLFAGGQKEEKIVLTWYQWFDAKAHET
                     ELLPTIAEFEKIYPNVKVELAAIPNEAYWDRLALDIASGVEGDIVTLDTGAGLMGYYS
                     QRPGGAFIPLDKYIKGYVLPDGTRLEEDILLIDSVKIEGNIMALPYIMFSAPMTAYRK
                     SVLKEAGIDPSELTRWTSYYEAAKKLTKDVDNDGKIDVYGFGHPQFAETISRWWHMHW
                     LWTAGGGIFPKEQPPYTADNLIFNSKENVTALSFLKKMLDETAPSGQKTVSELHSMFA
                     NGSLATVQIATWTLANFEEMISESEYRDDVAMVPFPAYDLDGKYLEPIYVSWGNPLAI
                     SSNCKHPKEAFDFIAFMHTKEAQKLAMRRASPTNILIYDEFAKIKPKQAEFVQKAQKY
                     EQRIVPDIVEWNQFDRAIQESVKSALLGTSSIEEALASGEEEMRRALK"
     gene            3286..4185
                     /locus_tag="PLB65_07320"
     CDS             3286..4185
                     /locus_tag="PLB65_07320"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ78372.1"
                     /translation="MSRIISRRHRDRIITIQFILPSIVIMSVFILFPIFKSIVMAFQE
                     WRLTSASTSHPFVGLDNIREVITLQHFPTMMKNTLIYTFFSVIGKMVIGLGVAVLLNR
                     EFVGRSFVRGLMLIPWAMPVVVVTNVFRIALDPDYGLLNVLLSSLPFVYGPIEFFSNK
                     ILALGTVITIGIWKNFPFVALMLLAILQSVPQDYYDAASIDGANFISQFRHITWPLIK
                     PIWNTLMILQILWTIKEFELIFLITQGGPSNGTNIIGIDIYLNAFRFYKVGVANAEGL
                     LLLVFCLIFATVYFRNLNKEGEL"
     gene            4185..5033
                     /locus_tag="PLB65_07325"
     CDS             4185..5033
                     /locus_tag="PLB65_07325"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ78373.1"
                     /translation="MMTGKRIASRVVLYLMVILVSIIITTPFVIMLSYSFRTSQNIFS
                     LKISLIPENPTIAAYKNAIFKYKFSGHGFGKWTINSLLTCGVATFVSIFVAAMCGYAI
                     SRFKFTGKRFLWFLIAITQTIPWIVILIPYYITASKLKLVNNLSFLSLTYTAVFLPTS
                     AWLFIGFFNNIPYEIEEAAKIDGCSTWGIFLRIIIPLSIASISAIALVAFVTGWSDYL
                     FASVFLKRASLWTLPLGLTSFRGEHQIQWAEIMAMSTLVTVPIVVLFVYLQKYLVDLM
                     AGGVKQ"
     gene            5056..6165
                     /locus_tag="PLB65_07330"
     CDS             5056..6165
                     /locus_tag="PLB65_07330"
                     /inference="COORDINATES: protein motif:HMM:NF022623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HOQ78374.1"
                     /translation="MNGFNFGTLSSLYRLRNSCSRSINPENPTGAVGEGGKAKSNLGV
                     GRKGKPYLDLPPSTETELAAIDGPGIINHIWITVQDRTAFGEFVLSDLIFRIYWDDET
                     VPSVDVPLGDFFCNGFGTKCQVNSLPIVVAPYGGFNSFFQMPFFKRARLTIVNQHPCE
                     IHAFFYTINYSLEESLPDNTAYFHAQWRRTKFSKPGVDHVILDGIVGEGHYVGTYLAW
                     SALERYWWGEGEMKFYIDSDTNYPTICGTGVEDYVGGAWGFYERNEEGIVQKYESTYC
                     TPYMGYPYYSTKDGTKVNVYGYDAVPMHGLYRWHILDPIRFTQKLKVTVQQIGHDGSR
                     LFERTDDVASTAFWYQREPHNPFPKLPAAEDRRPR"
BASE COUNT         2012 a         1146 c         1360 g         2152 t
ORIGIN      
        1 tccgcatcgc ggaaaaagcg aaaagcgatc ccgcctcgct gaagaaggcc ccggtctcga
       61 ccccggtagg ccggctcgac caggtgaagg ccgtcaagga ggcgagggtc cggtattaaa
      121 gtcattcttc aacctgattt gattcgaagg gcagggttat gatctatctg agttgagtgg
      181 atcattgttt tcatcaatat aatatacaat cttatttttt atagtagaat agccccaagt
      241 ataggcatga agatttggca tagaacaacc aactccctag gtaactggag agttttgtat
      301 tagtacatcc aaactacgta caatgttagc atgagcatgg ggctattgaa tgacctgtac
      361 aattatttcc aatcaccaaa agctttagga agtataccga cataccattg gactctccta
      421 attcaacttt ttttggggct cacttagagg cgcgaacccc attacatcgg gttaataata
      481 ctggttgtcg gtatatactt ggtaaagctt tttgtcggtt gctatttttc ggaaacttaa
      541 attttctgct gaagtggctg ttgaaaggct cctgtctgcc actttaaaat catagaatcc
      601 gccaaagctt ttcctaaaca atagaaagaa ggtgcaacac agcgaattgg aggtagccct
      661 ggataatgaa agaaatcact ttctgaatca taagctagaa aattgtaacc ctgaatggga
      721 gtgtcatgct tcagaaacag tgatgctagg atatagttag ctgaatagtc gcaggaaaaa
      781 atagtggtag ttgcgtcaaa attattaaag actgcatcag cttttgagag agcatccata
      841 tctgataaat gaatatagtt taccgatttt gctctaagat ttaccgcatc atagaatcct
      901 tgatatctcc tgttagcttc agggtaatat ccaaaagcat aaaaagattt ataatcattc
      961 tgtaataaaa tttttccagc ttggaaggca tgcttataat tatctgtctg aattgctgga
     1021 agtattggta attcgtcaat tatgatatca gaaattatat taactccttt atgaatagca
     1081 tgataaaaag gtaaagcagc atttttgaaa gatagggcaa ttattccatc acattgtaga
     1141 gactccaagt attctccgaa ttgaagggaa ttagtatctg cttcagtatt aataatcaat
     1201 tgagccacaa ctccggttcc gactaaccga tccagaactc catcaaaaaa tgctcgatga
     1261 aaccgttcat cttgcttgtt tgaaattaca actaaatctt tcttcagagt atctccgtgc
     1321 atatgtttat tgagaacagt aattccagat ttttgcttac ttaaaataag tccttcagcc
     1381 ttgagtgcgt gaacaccttt ttgagcagtt tgaaggctaa cattaaattt cgatgcaatt
     1441 tcacgaaccg ttaaatatcg atctccttca atatagttaa aaagaatatc tcggcggaga
     1501 tttcttagga aggaattaaa tattcttttg gacatcttaa attctccact tatgtcagtg
     1561 ttcttagatt aacttaatta taatgcaatg ttttgttatg atatacagac actatgtgct
     1621 aacggctcaa agattataac agatatttat cttatacaat ccaaattact gataatcaaa
     1681 ggataaaaac cataacaagt gtgattctaa taatagtaaa atcaaatata ttatgattaa
     1741 ctgttatgat atataaaaaa ttccattaga tatcataaga taaatttgac attcataaca
     1801 cagaaggtaa tactttttac atggtgttat tccattttaa ggaggaaagc tatgaaaaaa
     1861 gtatgtgtgt ttctgttagt agtattatta ctactaccgg cacttttgtt cgcaggagga
     1921 caaaaggagg aaaagattgt tttgacttgg tatcaatggt tcgatgcaaa ggctcatgaa
     1981 actgagcttc ttccaacgat tgcagaattt gaaaagattt atcccaatgt aaaagttgaa
     2041 ttagctgcaa tcccaaatga agcgtactgg gatcgtcttg cacttgatat agcaagtgga
     2101 gtagagggag atatcgtaac tctggacact ggagcaggtc ttatgggtta ttattctcaa
     2161 agaccaggtg gagcctttat tcctcttgat aagtatatta agggatacgt gcttccagat
     2221 ggaacgagat tggaagaaga tattttgttg attgattcag tcaaaataga aggtaatata
     2281 atggcacttc catacattat gtttagcgcg ccaatgactg cttatcgaaa atcagtttta
     2341 aaagaagctg gtattgatcc aagtgagcta actagatgga caagttacta tgaggctgcg
     2401 aagaagctta ctaaagatgt tgataatgac ggcaaaatag atgtatatgg ctttggacac
     2461 ccacaattcg ctgaaactat aagtcgttgg tggcatatgc actggctatg gaccgctggt
     2521 ggtggaattt ttcctaaaga acagcctcca tacactgctg ataacctgat ttttaacagc
     2581 aaggaaaatg tgactgcttt gtcattcctt aaaaagatgc tcgatgaaac agctccatct
     2641 ggacagaaga cggtaagtga acttcattcc atgtttgcaa acggttcttt ggcgactgtt
     2701 caaattgcta cttggacgtt agcaaatttt gaagaaatga ttagcgaatc tgaatacaga
     2761 gatgatgttg ccatggttcc tttccctgct tatgatttag atggtaaata ccttgaaccc
     2821 atctatgtat catggggtaa tccgcttgca atttccagta actgtaaaca ccccaaagaa
     2881 gcatttgact tcatagcatt tatgcatact aaggaggctc aaaaacttgc aatgcgtagg
     2941 gcatcgccga cgaacatact gatttatgat gagtttgcga aaattaaacc taaacaagct
     3001 gaatttgttc aaaaagctca gaagtatgag cagaggattg ttcccgatat cgttgaatgg
     3061 aatcaatttg atcgagctat ccaagagtcg gtcaagtctg ccctgctagg aacaagtagc
     3121 atagaagaag cccttgcctc aggtgaagaa gagatgagac gggcactgaa atagcattcc
     3181 tatttctatc atgctatcca gatagagctt aatccctatt gattctccta tgatggagaa
     3241 tcaataggtg gcttttgaaa gcataaactt aaattggagt aagtaatgtc ccgaattatt
     3301 agccgaagac atagagaccg gattattact attcagttta ttctcccatc tattgttatt
     3361 atgtccgttt ttatattatt tccaattttt aaatctatag taatggcatt tcaagagtgg
     3421 aggttgacat ctgcgtcaac ctcgcatcct tttgtgggtt tggataacat acgtgaagtt
     3481 attaccctcc agcatttccc tacgatgatg aaaaatactc tgatctatac ttttttcagt
     3541 gttataggaa aaatggtgat aggattgggg gttgctgtat tactcaatag agaatttgta
     3601 ggtagatctt ttgttcgggg cttaatgctc atcccatggg caatgccagt tgttgtggtt
     3661 acaaatgttt ttaggattgc acttgatcct gactatggtt tactaaacgt attgctaagt
     3721 tcactcccat tcgtatatgg ccctattgaa ttcttttcaa ataaaatact tgcgttagga
     3781 actgtcatca caattggaat atggaaaaac tttccttttg tagcgttaat gttgctcgca
     3841 atattgcaga gtgttccaca agattattat gatgccgcct cgattgatgg cgctaatttt
     3901 atatcccaat tccggcatat tacttggcct ttaattaaac ctatttggaa tactctgatg
     3961 attttacaaa tcttatggac aataaaggag tttgagctga tttttctaat tactcagggt
     4021 gggccatcaa atggaacaaa tattatagga atagatatat atctcaatgc gtttaggttt
     4081 tataaggtag gggtagcaaa tgctgaaggc cttttacttt tagtgttctg tttaattttt
     4141 gcaacagtct attttaggaa cctgaacaaa gagggagagt tataatgatg accggtaaaa
     4201 gaattgcatc gcgagttgtt ctatatctaa tggttattct tgtaagcatt atcattacaa
     4261 caccgtttgt cataatgctt tcttattcat tcaggacctc tcagaatatc ttttccctaa
     4321 agattagttt gattcctgag aatcctacaa ttgccgcata taaaaacgcc atttttaagt
     4381 ataaatttag tgggcatggg tttgggaaat ggacaatcaa cagtctgctg acatgtggag
     4441 tagcgacgtt tgtttctatt ttcgtagctg caatgtgtgg gtacgcaatt agtcgtttta
     4501 aatttacagg aaaaagattt ttatggtttc tcattgctat cacccaaaca ataccttgga
     4561 ttgtaatctt aattccctat tacattactg caagtaaact taaactggtt aacaatctta
     4621 gttttctcag tctaacgtat actgctgtct tcctgccgac cagcgcttgg ttgtttattg
     4681 gtttttttaa taacatccct tatgaaattg aagaagctgc aaaaattgat ggatgttcaa
     4741 cttggggtat ttttctgcgg attattattc ctctttcaat agcgagcatt tcagcgattg
     4801 ctttggttgc ctttgttact ggttggagtg attatctttt cgcttctgta tttcttaaac
     4861 gagcttcttt atggacatta cccttaggtc ttacaagttt cagaggagag caccagatac
     4921 aatgggctga aataatggca atgtctactt tagtaaccgt tccaatcgtt gtacttttcg
     4981 tctatcttca gaaatatcta gtcgatttaa tggctggtgg agtaaaacaa taatcattta
     5041 tatgtaggag tgaatatgaa cggatttaat tttggaactt tatcatcgct gtatagattg
     5101 agaaatagtt gttcaaggtc tattaatcca gagaacccca ctggagcagt tggggaagga
     5161 gggaaagcga aaagcaattt aggtgttgga aggaaaggaa aaccttatct tgatcttcct
     5221 ccatctacag aaactgagtt ggcagccatt gatgggcctg gaattattaa ccatatttgg
     5281 attactgtcc aagatagaac agcttttgga gaatttgttt tgtcagactt gatttttaga
     5341 atctactggg atgatgaaac cgttccttca gttgatgtac cgcttggtga ttttttttgc
     5401 aatggatttg gaacaaaatg ccaagtgaac tcacttccaa tcgtggtagc cccgtacggg
     5461 ggttttaata gtttctttca aatgccattt ttcaaacgag cacgattaac tatcgtaaac
     5521 caacatccat gtgaaatcca cgcttttttt tatacaataa attactctct tgaggaatct
     5581 ttgccagaca atacagcata ttttcatgct caatggagaa gaaccaagtt tagcaagcca
     5641 ggtgtagacc acgttatttt ggatggtatt gtaggtgaag gccattatgt tggaacatat
     5701 ttagcatggt ccgcgttgga gcgttattgg tggggtgaag gtgaaatgaa attctatatt
     5761 gatagtgata ccaattatcc cactatttgc ggtactggtg ttgaagatta tgttggaggg
     5821 gcttgggggt tttatgagcg aaatgaagag ggtattgttc aaaaatacga gtcaacgtat
     5881 tgcacaccct atatggggta tccttattac tccaccaagg acggaacaaa agtaaatgtt
     5941 tatgggtatg atgcagttcc gatgcatggg ttgtaccgct ggcatatttt ggatccaatt
     6001 agatttaccc agaagctaaa agttaccgtt cagcaaattg ggcatgacgg aagtaggctc
     6061 tttgagcgga cggatgatgt agctagcact gccttttggt atcaacggga acctcataat
     6121 ccttttccta aactcccagc agcagaagat agacgacccc gctaacactg tgaatatttg
     6181 tatacctgat atacttcagt gaatataagt aatgttcaac atattgcacc gatgtcatgg
     6241 tctttaggac atcggtgtaa aattaattat atttggggta atccactaca tacatagagc
     6301 tatattttta ctgtgctaat tttgatatag tgctgattaa cagaactcac taactattca
     6361 attctgttgg ggctgaatag ttttagtgaa tagagaagtg cagtttttga atactgcttt
     6421 aaggtgctag catttgctga caatgattat caagtctaat caaacaaatc tatgttgttg
     6481 tctactccgc aatttatttc tgtgatcgtt ccctatcata aattaattca ttttcgatta
     6541 ctctcggaat tcttatattc gaggcacctt aaagcaggag ccggatcggg taaatgttga
     6601 taattgcttc ctcacggagt atcctaggtg tatgaaaaga cttctggtta cccaaggttt
     6661 gctggtggat
//
LOCUS       DAOSTH010000083         6588 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_10761_length_6588_cov_16.430277, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000083 DAOSTH010000000
VERSION     DAOSTH010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6588
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10761_length_6588_cov_16.430277"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            193..1275
                     /locus_tag="PLB65_07335"
     CDS             193..1275
                     /locus_tag="PLB65_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOQ78375.1"
                     /translation="MIDKIKHSRKIVVAFVSVPILILLLAMVLIAIKQNLLEKKYTYY
                     STVVNAMGLSNQTPVLYRGFEVGRVRNFSLADDGNIGIEFYILNRYKNIMVKGSVLVR
                     NTNPLTGKTTLEFIRNPNSYEPLPVESNIPSSDFSEGKQQLKLIAPTLSDPISVIIAN
                     LAQLSTAINEDNNADKGSIPRMLVNLADASEKANAGLDNVQNITRELSILTMNLNKDN
                     NPESGVLLRTLNNLAELTYNLNNRMTELEDILASVQIALDNYKQPDSLLIKLLDPNSD
                     KILKPLSETLHNLETATAEVSQILATVNNPELRMLLNNLNDSLAKARKTLEALNNNPL
                     LRKGISPASTQATPSSKRMHEVPNAQ"
     gene            1265..2128
                     /locus_tag="PLB65_07340"
     CDS             1265..2128
                     /locus_tag="PLB65_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78376.1"
                     /translation="MRSKWLLIILLILSACSSIKIPECPQAKIDADNQLRFASQLEST
                     KDYSAAGESYNFLLNSYRSFADVKGMLNCLSGLARIALEENDDKAYLEKRMQMAELIQ
                     TSANELNYYLLLLDIYYYQRENDLVAISEKAIITKDLPWEVSVNLATAKLQADSFLGT
                     ATGKQVQELNTLSNKLEKQLKKGKNANNELLSSAWYALAYYYFRNSNYKNAYAFAHKA
                     QELDYRYGNFSGMAHCLWLKGQIAFAENKISEAQSNWKKAEGIFISLNDTQALQKLRQ
                     DINKLPQEQMK"
     gene            2205..3482
                     /locus_tag="PLB65_07345"
     CDS             2205..3482
                     /locus_tag="PLB65_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOQ78377.1"
                     /translation="MKLLRSVSLPTSSTALKRVNVLFEDKILKLSTDDIEVEEDVEII
                     EAKGLLMLPGAIDPHTHILTQDSENASDLSRFTKIALEGGYTTLAELSYHTPNPIFDN
                     RSLKKKMAFINANSFTSMALWGHIDMGDYPYHSESAQEIWSKGVVGISLAVPSPNPAI
                     ADISFTEIMDLFLDIYESDTSFAFQGYDNENHQAFSSTAQLEAIKKILRRMQENPIHI
                     PRVSSYPTIEFINSVSKRSDISFAISLVDMMALYNPDIFETPFNCDFTDYADLLFELL
                     RTNKIYLLSNCAAPQETNPEYCELFQGSDPEMMSYSYLWVLSELWKKRRIPLATCIKM
                     TSENAAKRLGIYPEKGSLDPGSDADFVLYDPETTTLITGNKGKTTELIGGFKAVYLKG
                     NQVAGENCTTQKQGSFFARSTNPKRRHNKTTWI"
     gene            3914..5242
                     /gene="ssnA"
                     /locus_tag="PLB65_07350"
     CDS             3914..5242
                     /gene="ssnA"
                     /locus_tag="PLB65_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOQ78378.1"
                     /translation="MNRYILQGGTIITFDSQNPLLEEQAILIDDGMIVKIGDLEDFRD
                     EVCERIDVEGKFILPGLINAHHHFYSTFATGLTKTAVSHNFSEVLSNLWWRLDKNLLE
                     EDIYYSALVSALTSIRKGTTTIIDHHSSPFCVQNSLSQIAKAILDTGIKASLCFEVSD
                     RDGAEIAQAGIEENYQWLKHCQNNPNPYLKGLFGLHAAFTLSDKTLSQIAEKVVELNC
                     GIHIHTAEAETDEKYNVEHYGKRVVERLNDFNLLNSKSILAHCVYLNAKELILAAEKG
                     AAIVTNPQSNLNNAVGIADVCKMKELGVTVGLGTDAMTNNMLEELRIGVWAQHLKQND
                     PSRGFTEITSALVFNNPIITQKYWGEKHGTIKEGSPADIIVVDYEPYTPLTEANWMGH
                     LVYGISQAKVDVTICQGRILLWEGELLLNLDEQEIIAKAKECAEKLWDRM"
     gene            5381..6577
                     /locus_tag="PLB65_07355"
     CDS             5381..6577
                     /locus_tag="PLB65_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOQ78379.1"
                     /translation="MYKEIYERAKHYEDTNARFLMDIVKIPSFSTKEKEVAKRILKEM
                     QEIGLNEAYIDPLGNVIGRLGCGKKVIAFDAHIDTVYPGDLSLWDFDPFDAHLKDGKI
                     WGRGTVDQKGGMASMMTAARIIKDLNLDKDLSIYFTGTVMEEDCDGLCWQYILNEGEI
                     KPELVVITEPTNLNIYRGHRGRMEMEVSVKGLSCHGSAPERGDNAIYKISRIALEIEK
                     LHQHLRSDDFLGKGSICVTQVFFTGPSQCAVPDSARLHLDRRLTWGENKESAVAEVQD
                     ACIKAGYPDAQIEVLSYAEKAYTGLVYPTEKYYPTWVTPLDSAYVQNAADAYSKTLEI
                     EPVIDKWTFSTNAVAIAGMKGIPCIGLGPGNEIYAHSPNEAIPIEHLTKAAAFYAALV
                     YRLAQG"
BASE COUNT         2133 a         1171 c         1327 g         1957 t
ORIGIN      
        1 tagttcaaag tttttcattc ggtgttttcg gtgccctcgg tggctaaata ctcttctata
       61 atcctctttt ctaaaattaa atccttgatt ctgaagaaag actggacaaa taatatcagc
      121 aaaacagcag taaaagcttg acaaaaaaca gaacttaaaa ataataataa taaggcgaaa
      181 tagagttaaa ttatgataga taagattaag catagccgga agatcgttgt tgcctttgtc
      241 agcgtaccaa ttttgatatt gttactggca atggttctta ttgctatcaa gcagaatctg
      301 ctggaaaaga aatatactta ttatagcact gttgtaaatg caatgggttt atctaaccaa
      361 actcctgttt tgtatagagg ttttgaagtt ggtagagtgc gtaatttctc gcttgcggat
      421 gacggtaata tcggcataga gttttacatt ttgaatcgtt ataaaaatat aatggttaag
      481 ggatcagttt tggttcgtaa taccaaccca ttaactggca aaacgaccct ggagtttatt
      541 cgcaatccta attcatatga accattacct gtggaaagta atattccgtc ttcagatttt
      601 tcggaaggca aacagcagct aaaattaatt gctcctacat taagtgatcc gatttctgtt
      661 attattgcaa atttggcgca gctttctact gctatcaatg aagataataa tgcagataaa
      721 ggttccattc cgcggatgct ggtgaactta gcagatgctt ctgaaaaagc aaatgcaggt
      781 ttggataatg tgcaaaacat aaccagagag ctttctattt taacgatgaa cctaaacaaa
      841 gataataatc ctgaaagtgg agttctgcta aggactttga acaatctggc agagctaacc
      901 tataatttaa ataacagaat gactgagtta gaagatatat tggcaagcgt gcaaattgct
      961 ttagataatt ataaacaacc cgattcgcta ttaataaaac ttcttgatcc taatagtgat
     1021 aagatcttaa agccactttc tgaaacttta cataatttgg aaacagcaac agctgaagtg
     1081 tctcaaattc tggcaacggt taataatcct gaacttagaa tgctgcttaa taatctgaat
     1141 gacagcttgg ccaaagcccg taaaactctg gaagcattaa ataataatcc tcttttgcga
     1201 aaaggaataa gtccggcttc aacacaagcc actccttcct caaaaagaat gcatgaggtt
     1261 cccaatgcgc agtaaatggt tattgataat cttattaatc ctttctgcct gttcttctat
     1321 aaaaatacct gaatgtccac aggcaaaaat tgatgctgac aatcaattgc gttttgcttc
     1381 acaattggaa tcaactaaag attattctgc tgctggagaa agctataact tcttactaaa
     1441 cagctatcgt tcttttgccg atgtaaaagg gatgctgaat tgtttaagtg gtttggctcg
     1501 gattgcactg gaagaaaacg atgataaagc ttatctggaa aagagaatgc agatggcgga
     1561 actgattcaa acttccgcta acgagctgaa ttactatcta ctgcttttgg atatttatta
     1621 ttatcagcga gaaaatgacc tcgtagccat ttccgagaag gctattatta ctaaagattt
     1681 accttgggaa gtttccgtaa atttggcaac agcaaaattg caggcagata gctttctggg
     1741 aactgccacc ggcaaacaag ttcaggaact aaacacttta tccaataaac tggaaaagca
     1801 gctgaaaaaa ggcaaaaacg ctaataatga attactttct tctgcctggt atgcgcttgc
     1861 ctattattat tttcgaaatt ctaattataa aaatgcctat gcctttgccc ataaggctca
     1921 ggaactggat tatcgctatg gtaatttttc tgggatggct cattgcttgt ggctgaaagg
     1981 tcaaattgcc tttgccgaaa ataaaatatc cgaggcccaa agcaattgga aaaaagcaga
     2041 aggtattttt atttccttga acgatacaca agctctgcaa aaactacgac aggatattaa
     2101 taaactgccg caagagcaaa tgaaatgaac agcaaaaaaa agcggctggc agaattattt
     2161 accaattcag gtgaatttat tttaccgcca aaatgatatg aattatgaaa ttacttagat
     2221 ccgtttccct acctacttct tcaacagctc tgaaaagagt gaatgttttg tttgaagata
     2281 aaatacttaa gctttccaca gatgatattg aagtggaaga agatgttgaa ataattgaag
     2341 ctaagggact ccttatgctt cctggtgcta ttgatcctca cactcatatt ttaacccaag
     2401 attctgaaaa tgcttcagac ctatcccggt ttacgaaaat tgccttggaa gggggatata
     2461 ctaccctcgc ggaattaagc tatcatacac ctaatccgat ttttgataac cgctcgctga
     2521 aaaaaaagat ggcttttatc aatgccaatt cttttacttc tatggctctt tgggggcata
     2581 ttgatatggg cgattatcca tatcattcag aatccgcaca ggaaatttgg agcaaagggg
     2641 ttgtaggaat atctttagct gttccatccc ccaatccagc aattgctgat attagcttta
     2701 cagaaataat ggatctcttt ctggatatct acgaaagcga tacttccttt gcttttcaag
     2761 gatatgacaa cgaaaatcat caggcattta gttctactgc tcaactggaa gcaataaaaa
     2821 aaatcttacg cagaatgcaa gaaaatccaa ttcacattcc gcgtgtttct tcatatccta
     2881 caattgagtt tattaatagt gtttccaaaa gaagcgatat ctcttttgcc atctcccttg
     2941 tggatatgat ggctctttac aatcctgata tatttgaaac tccctttaat tgtgatttta
     3001 ccgattatgc tgatctgctt tttgaactgc tgcgcactaa taaaatttat ctgttatcca
     3061 attgtgccgc tcctcaggaa acaaatcctg agtattgcga actctttcaa ggtagcgatc
     3121 cggaaatgat gagttattca tatttgtggg tactttctga actttggaaa aaaaggagaa
     3181 ttccgcttgc tacctgcatc aaaatgacca gcgaaaacgc tgctaagcgt ttaggaatat
     3241 atcctgaaaa gggttcttta gatcctggct cagatgccga ttttgtgctt tacgatccag
     3301 aaacaacaac tttgataaca ggcaataaag gaaaaacaac agaactgata ggtggcttta
     3361 aagctgttta tctaaaagga aatcaagttg ccggagaaaa ctgcacaact cagaaacagg
     3421 gctcattttt tgcccgaagc acaaatccca aacgtagaca taataaaact acctggatct
     3481 aaatgaatct gtatgtagtt tatccgaaag gagtaaacac ctgcggaaag acaaatatat
     3541 gaggatagta cttaatatct acccaaatta taccaaatag agaaaataag ctagccagtt
     3601 cgtccctgga aaatataccg tagttcacca acggacttta tttatacctg ttttttgaaa
     3661 atattagttc agtgcctcag tggtaggaaa tgtccataaa ctatttggct ttctaattca
     3721 gatttttttt aagaggcaaa attcctgatg aattgtaaaa acggtatgtt actacttcct
     3781 tattaactga acttattaca aacggagatc ctctttttct ttactctctc ttttgaaaca
     3841 aacaatcaaa cttgacaaat atagcccgat ctcatttatt tacataagag gaatttataa
     3901 gggaggaata gtgatgaaca gatacatact tcagggtgga acgattatta ctttcgatag
     3961 tcaaaacccg cttttggaag agcaggcaat tttgattgat gatgggatga ttgttaaaat
     4021 tggcgatctg gaagatttta gagatgaggt ctgtgaacgc attgatgtag aagggaaatt
     4081 tatcttaccg gggctcataa atgctcatca tcatttctat tctacttttg ccactggctt
     4141 aacgaaaact gctgtttccc ataactttag tgaagtttta agtaatcttt ggtggcggct
     4201 cgataaaaac cttctggagg aggatattta ttacagtgct ttagtttcag cgttaacatc
     4261 tatccgcaaa ggaacaacca ctataattga tcatcattct tctccttttt gcgtgcagaa
     4321 ttctttgtct caaattgcca aagcgatttt ggatacaggc atcaaggcat ctttgtgttt
     4381 tgaagtttca gacagagatg gtgcagaaat tgcccaagcc gggatcgagg aaaattacca
     4441 gtggctaaaa cactgccaaa ataatcccaa tccttatttg aaaggacttt ttggtctgca
     4501 cgcggcattc actctttccg ataaaaccct ttcccaaata gctgaaaaag ttgtagaact
     4561 taattgtggt atccatattc atactgcaga agcggaaaca gacgaaaaat ataatgtgga
     4621 acattacggt aaaagagttg tggaacgctt aaacgatttt aatctactaa atagtaaaag
     4681 cattctggca cattgtgttt atttgaatgc caaggaactt attttggcgg cggaaaaagg
     4741 agctgccata gttactaatc cgcaatccaa tttaaataat gccgtaggaa ttgccgatgt
     4801 ttgtaaaatg aaagaactgg gcgtaacagt tggtctcggt actgatgcaa tgaccaataa
     4861 tatgctggaa gaattacgca ttggagtttg ggcacaacat ttgaaacaaa atgatccttc
     4921 tcgtggcttt acagaaataa cctctgccct tgttttcaat aatccaatta taacgcaaaa
     4981 atattggggt gaaaaacacg gaactataaa agaaggaagt ccggcagata ttattgtggt
     5041 agattacgaa ccctatacac ctctaacgga agcaaattgg atgggacatc tggtttatgg
     5101 aatatcccaa gcgaaagtgg atgtaactat ttgccaggga agaattttgc tgtgggaagg
     5161 agaattactg ctaaatcttg atgaacaaga gataatagcc aaagcaaaag agtgtgcaga
     5221 aaaactttgg gataggatgt gaagaaagtg gggtggggat tagatatagt tttaggtttt
     5281 attattatgt aataatcctt gaagtcccta taatcctgtt catcccagac ctatttattt
     5341 taggtaatct gcgtgaaaga taatttaagg agaagcgatt atgtataaag aaatttatga
     5401 gcgagccaaa cactatgaag ataccaatgc tcgtttcctg atggacatag taaaaatacc
     5461 ctcattttct actaaggaaa aagaagtagc gaagcgcatc ctgaaagaaa tgcaagaaat
     5521 aggcctgaac gaagcatata ttgacccttt gggaaatgtg ataggacgcc tgggctgtgg
     5581 gaaaaaagtg attgccttcg acgcacatat agatactgtc tatccgggag acctttcgct
     5641 gtgggatttt gatccttttg acgctcattt gaaagatggt aagatttggg gtcgcggaac
     5701 cgttgatcaa aaaggtggaa tggcaagtat gatgacagcc gcgcgcatta tcaaagacct
     5761 gaatttggac aaggatttaa gcatatattt taccggtacg gttatggaag aggattgtga
     5821 tggcttatgt tggcaatata ttttaaacga aggcgaaatt aagcccgaac tggttgttat
     5881 tacagaacct acaaatctta atatctatcg tggtcatcga ggcagaatgg aaatggaagt
     5941 aagcgtgaaa ggactttcct gtcatggttc cgctcccgaa cgcggagata atgccatata
     6001 taagatttcg cgtattgctt tggaaataga aaagttgcat caacacttgc gttcagatga
     6061 ttttttgggt aaaggcagta tctgtgttac tcaggtattt tttactggac caagtcaatg
     6121 tgcagttccg gatagtgctc gcctgcattt agaccgacgc ttaacttggg gagaaaataa
     6181 agaaagtgct gttgccgaag ttcaagatgc ttgcataaaa gctggctatc ctgatgccca
     6241 aatagaggtt ctatcctatg cagaaaaggc atatacggga ttggtttatc ccaccgaaaa
     6301 atactatcca acttgggtca cgcctttgga ttctgcttat gtgcaaaatg cggctgatgc
     6361 ctattctaaa actctagaga tagagccagt tatagataaa tggacttttt ctaccaatgc
     6421 tgtggcaatt gccggtatga aaggaattcc ttgtattggc ttaggacctg gcaatgaaat
     6481 ttatgcccat tcacctaatg aagcaatccc gattgaacat ttaacaaagg cagctgcgtt
     6541 ttatgctgct ttggtatata gattggcgca gggatgaaga ggtcgagg
//
LOCUS       DAOSTH010000084         6563 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_10807_length_6563_cov_11.893516, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000084 DAOSTH010000000
VERSION     DAOSTH010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6563)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6563
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10807_length_6563_cov_11.893516"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(701..2197)
                     /gene="lysS"
                     /locus_tag="PLB65_07360"
     CDS             complement(701..2197)
                     /gene="lysS"
                     /locus_tag="PLB65_07360"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOQ78380.1"
                     /translation="MPDNSNQFIRLRKEKLEKIKSLGVDPYPIDSNRTHYISALLEDK
                     DKWIAQAEEITIVGRLVAMRRQGKIGFGNIEDISGKIQIYVAQNLIGEENYDLFKLCD
                     AGDFIQATGILFYTQTGEYSLKCNAIKLLSKNIRPLPAVKEKIVDGKKVRYDEFSDIE
                     LRYRKRYLDLLLNPEHRKVFETRAKIITAIRSFMDSRGYVEVETPILQPLYGGANARP
                     FVTHHNTLDMDLYLRIATELYLKRLIVGGFERVYELGKDFRNEGMDRTHNPEFTMIEF
                     YEAYSDLRGMMDIAEDLIKHLAMDVIGKDTFLYHNHQVCLSGNWKRVSMPDLVSEFVG
                     QDVMNMDFATLQAFCKHKGLEIPAGSEKGKLIALIFEEFVEKTLIQPTFVCDFPKEVS
                     PLAKAIPDNPFLADRFELYIAGGEFANAFSELNDPLEQRMRLEEQAKLRDLGIDDANV
                     VDEDFLEALEYGMPPMGGQGIGIDRLVMLLTENDSIKEVILFPQMKTT"
     gene            complement(2205..3716)
                     /locus_tag="PLB65_07365"
     CDS             complement(2205..3716)
                     /locus_tag="PLB65_07365"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOQ78381.1"
                     /translation="MPNKLREDSNKATMKEMKEEYLRMLEESFQNTAEIKKGDVVEAP
                     IVSIGDQYLILNLGGKFDAYAEIGEFSDSKGDLPYRVGDNLKGYVVDQNENGYVIAKS
                     LTRQYVDKQSILDAFEKKIPVQGKVNSPTKGGFNVDILGARAFCPISQISLHPVEDTA
                     QFIGKNLDFLVIECSENCRRLVVSHRQLEEMKEKELKEEALAKLKVGDVIKGKVLRMT
                     NFGAFIDLGGIEGLMHVSEISWQHVVKPQDELKKGQDIEVQILDIKGDKIALSRKALI
                     ENPFLQALNELHEGDTINCRILRLHSFGAFAELKPGVEGLIPISEMSRNRNISHPREI
                     VKEGDWVAVQILRIDEDTRKISLSLKALEEDPWDKIDEIIELDNPFEGIVESSTNFGV
                     FVTITDGVTGLLPRSRVRKNDSFKTGDPVNLKVTAIDKDNHRITLDYTDRTPEELFEE
                     QNRSANYNQRESRNENPRSGNQRRGSRNRNDEEWRKYANQKVNSAADNPFKDL"
     gene            complement(3753..6323)
                     /locus_tag="PLB65_07370"
     CDS             complement(3753..6323)
                     /locus_tag="PLB65_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOQ78382.1"
                     /translation="MKEYSNLKQGLTTQEVEDKLKREGYNELPSSNARSIFKLMWDVI
                     SEPMFILLVSCAVIYFFLGDLQEALVLTASVVIVMSIELFQERKTEKALEALKNLSSP
                     RALVIRNSEQIRIPGREVVTDDLIILAEGDRIPADAYILENNSLSVDESLLTGESVPV
                     RKRIWNKEERPINPGGDDLPFVYSGTLVVSGRGIAQVFATGEKTELGKIGKSLEAITE
                     EETRLSKETGKLVKYFAAFALLICVVVVIVFYITRKHLVNALLTGITLAMSVLPEEFP
                     VVLTVFMALGAWRMSRKNVLVRRLQAIESLGSATVLCTDKTGTITYNKMTLQKIVSSD
                     ENIDLKSADKISNSIYTILYHSVLSSMREPFDPMEKAIHSALNEIEPSNYQELQNYQE
                     IKEYPLTDELRAMAKVWKLPDKTDYLVSLKGSPEAVIKLCALNSEEIEPILNQVSDLA
                     EEGLRVLGVAYCEVVPNVLPEKMTDLPFHYLGLIGLADPIRETVPEAVKMCYKAGIRI
                     IMITGDYIGTAKAIGKQIGLKNVDNIIVGSELDQMTDLELAEKCKQVSIFARVVPHQK
                     LRIVQALKANKEIVAMTGDGVNDAPALKAANIGISMGLRGTDVAREASAIVLTDDDFS
                     SIVSAVRMGRRIYDNLRKSFTYVFSLHIPIAGMAMIPAAFSNYPIIYFPVHIAFLELI
                     IDPACSIVFEMEKEEDDIMERPPRSIDEKLFGTRRLIIGALQGISVLILLVLIYFWGI
                     KSGMGDNHLRAHIFTTLVFSNLMLITTNRSWKKNLFQIIRTPNKGMTYLFLGVFTIVL
                     LMTQAPYITNIFHLESMSLLELLTCLVLGNISVAWFEIYKLIVNKKAKKQKPLQKEAL
                     "
BASE COUNT         2001 a         1347 c         1070 g         2145 t
ORIGIN      
        1 cttgatttac gcttggaggt aagagcaaaa agcgaagctt gcttccgata ttctgtctat
       61 tatagtagca aatacttgaa gatagataag tacattatga taataattaa tagcaaccta
      121 caattttaca gttcaacgaa aacgggctaa acaatccttt cttagcgaat aaactaaagg
      181 ttactaacgg acttagccca gctaatacag ttacaaatgg tagaaaactg gaagtcctta
      241 caaaattagg attatgtcta atattcagat gttagaatag gataaatctc cactgcattg
      301 cagccaaaaa aatcaaaaca taactaactg ttttaattat aattatgaaa cactctaaga
      361 atataatcct ctcaatccta caatcctgaa atccttgttt ctaaaaatca atccctaaaa
      421 tctcccaaat cttgttcagc ccatatctat tatcttaaat tcgtcatcgt cgctattatt
      481 atgtcttctt tgaagggatt tttaataagc cgtccgaagt tataagtcag gtatattgca
      541 tttacaaaat aggaaacatc attatagaaa ggaagtaagc actgctttaa tattcatata
      601 atccaaagga cttgcttttt tgagtatttt ttcacaatag ccataaatta gagagattat
      661 atccttgctt ttatattgat aaatggtgtt ataaaattga ttaggtggtt ttcatttggg
      721 ggaaaaggat aacttcttta atagaatcgt tctccgtaag taacataacg agacggtcta
      781 ttccgatacc ctgtccaccc ataggaggca ttccatattc aagagcttcc agaaaatctt
      841 catccacaac attggcatcg tcaattccca gatctcgtaa ttttgcttgt tcttccaagc
      901 gcattctttg ttccaaggga tcattcaatt cgctgaaggc attggcaaat tcacctcccg
      961 ctatatatag ttcaaagcgg tctgctagga aaggattatc gggaattgcc ttagctaaag
     1021 gagaaacttc tttaggaaaa tcacaaacga aggttggttg aataagggtt ttctctacaa
     1081 attcttcaaa aatcaatgca atcaatttac ctttttctga gccggcagga atttctagtc
     1141 ctttatgttt acagaatgcc tgcaatgttg caaaatccat attcattaca tcctgtccta
     1201 caaattcgct aaccagatca ggcattgaaa ctcttttcca gtttccgctt aaacatactt
     1261 ggtggttgtg atacaagaat gtatctttgc cgataacatc catagccaag tgcttaataa
     1321 gatcttcagc aatatccatc attcctctca gatcagaata tgcttcataa aactcgatca
     1381 tagtgaattc aggattgtga gttctatcca tcccctcatt acgaaaatcc ttacccagct
     1441 cgtaaacccg ttcaaaacct cccacaatta atcgtttcag ataaagctct gtagcgattc
     1501 ttaaatataa atccatatct aaagtattgt gatgagtaac gaaaggacgg gcatttgctc
     1561 ctccgtaaag tggttgtaaa ataggggttt ctacttctac ataaccgcga ctatccataa
     1621 aactacgaat agcggtaatt atttttgctc gggtttcaaa cacttttcta tgctcgggat
     1681 tcagtaaaag atcaagataa cgcttacgat aacgtaattc aatatcagaa aattcatcgt
     1741 agcggacttt cttaccgtca actatttttt cttttacagc tggcaagggt ctgatgtttt
     1801 tagagagcag tttgatagca ttgcatttca gagaatattc acctgtttga gtgtaaaata
     1861 aaattcctgt cgcttgtata aaatcaccag catcacatag cttgaaaagg tcgtaatttt
     1921 cttctcctat aagattttgt gctacataaa tctgaatctt gcctgaaata tcctcaatgt
     1981 ttccaaaacc tattttgccc tgcctacgca tagctacaag acgccctaca atagtaattt
     2041 cttcagcttg tgcaatccat ttatctttat cttccaaaag ggcagatata taatgagtcc
     2101 tattggaatc aattggataa ggatctaccc ctagtgattt aatcttttcc agtttttctt
     2161 tgcgtaaccg gataaattga ttagaattat ctggcattac tattttataa atccttaaag
     2221 ggattatcag cagcagaatt tactttttga ttggcatatt tacgccattc ttcgtcgttg
     2281 cgattgcgtg aacctctgcg ttgatttcca ctgcggggat tttcatttcg tgattctctt
     2341 tgattgtagt ttgcagagcg attttgctct tcaaatagtt cttcaggtgt cctatctgta
     2401 taatcaagag ttattctatg gttatcctta tcaatagcgg ttacttttaa atttacagga
     2461 tcacctgttt taaagctgtc attcttacga actcgagacc ggggtaaaag ccctgtaact
     2521 ccatcagtga tggttacaaa aactccaaaa tttgtagaac tttctacaat accttcaaag
     2581 ggattatcca gttcgataat ttcgtctatt ttgtcccagg ggtcttcttc caatgctttc
     2641 aaagaaagag atattttacg ggtatcttcg tcaattctta aaatttgaac agctacccaa
     2701 tcaccttctt tcacgatttc gcgaggatgc gaaatattgc gattacggct catttcggaa
     2761 ataggaatta aaccttccac accaggtttt agttcggcaa aagcaccaaa actatgtaaa
     2821 cgcagaatgc gacagttaat tgtatctcct tcatgcagtt cgttcagtgc ctgtaaaaaa
     2881 ggattctcta ttaatgcctt acgagaaagg gcaattttat cacccttaat atccaaaatt
     2941 tgcacttcaa tatcctgacc ctttttcagc tcgtcttggg gcttaacaac atgctgccag
     3001 gaaatttctg aaacatgcat cagaccctca attccaccta aatctataaa agcgccaaaa
     3061 ttggtcatcc gcaaaacttt acctttaatc acatctccaa ccttcaactt ggctaaggct
     3121 tcttctttca gttctttttc tttcatttct tccagctggc gatgtgaaac tactaatcga
     3181 cggcaatttt ctgaacattc aataacaaga aaatctaagt tcttacctat aaattgagct
     3241 gtatcttcaa ccgggtgtaa ggatatctgt gaaattggac agaatgctcg tgctcccaga
     3301 atgtcaacat taaatcctcc cttggtagga gaattaactt ttccctgcac agggattttt
     3361 ttctcgaaag catcaagaat ggattgctta tctacatact gtcttgttaa acttttagca
     3421 attacatatc cattttcatt ttggtcaaca acataaccct ttaaattgtc tcccactcta
     3481 tatggaagat ctccttttga atcggaaaat tctccaattt cagcgtaagc atcgaatttg
     3541 ccgcctaaat taagaataag atattgatca ccgatgctga ctatgggagc ttcaactaca
     3601 tccccttttt taatctcggc tgtgttttga aaggattctt cgagcattcg aagatattct
     3661 tctttcattt ctttcattgt agctttattg ctatcttctc gtagcttgtt gggcataact
     3721 atctcctaaa ggcatttcag aaaaccacct tttcagaggg cttctttttg taaaggtttt
     3781 tgtttctttg ctttcttatt cactatcagc ttgtatattt caaaccaagc gacgctgatg
     3841 ttccctaaaa ctaaacaggt aagtaattct aatagagaca ttgattctaa gtggaagata
     3901 ttggttatat aaggcgcttg agtcatcaac agaacaatgg tgaaaactcc caaaaataaa
     3961 tatgtcatac ctttattggg agtgcggata atctgaaaca agttcttttt ccagctacga
     4021 tttgtagtga ttagcatcaa attggaaaag accaaagtgg taaaaatatg cgctctcaaa
     4081 tgattatctc ccattccgct tttaattccc cagaaatata tcaacactaa taaaattaga
     4141 acgctaatgc cttgtaaagc accaataatt aatcttctag tgccaaacaa cttctcatca
     4201 atagagcgag gaggtctttc cataatgtca tcttcttctt tttccatttc aaaaacaata
     4261 gagcaggcag ggtctatgat taattctaaa aaggcaatat gaactggaaa atagataatc
     4321 ggataattag aaaatgcagc aggtatcatt gccatcccag caatgggtat atgtaaagaa
     4381 aacacataag tgaaggactt tcgcaggtta tcatatatcc ttctacccat ccttacggca
     4441 cttacaattg aagaaaaatc gtcatctgtt aaaacaattg ccgatgcttc acgcgctaca
     4501 tccgttcctc ttaaacccat agaaatacca atattggctg ctttcaaagc aggggcatca
     4561 ttaactccat ctcctgtcat agccacaatt tctttgtttg cctttagggc ttgcacaatt
     4621 cttaatttct gatggggaac aacccgggcg aatatagata cctgtttaca tttttctgcc
     4681 aattccagat cggtcatttg gtctaattca ctaccgacaa tgatattatc tacatttttc
     4741 agcccaattt gtttaccaat cgcttttgcc gttccaatat aatctccggt aatcataata
     4801 atcctgatgc ctgctttgta acacatctta actgcctcag gaactgtttc tctaatcgga
     4861 tctgcaagtc cgatcaaacc caaatagtgg aaaggtaaat cagtcatttt ttccggtaag
     4921 acattgggaa ctacttcaca ataggcaact cctaaaactc ttagcccttc ttctgccagg
     4981 tctgaaactt gatttagtat cggctcgatt tcttcagaat tgagagcaca gagtttgata
     5041 actgcttcag gagatccttt tagcgaaaca agataatctg tcttgtcagg taatttccag
     5101 accttagcca tcgctcgtag ttcatcagtt aaaggatact cctttatttc ttgatagttt
     5161 tggagctctt gataattaga tggttcaatt tcatttaaag cactgtgaat tgccttttcc
     5221 atagggtcaa agggttctct catactgctc aacacactgt ggtaaagaat agtgtagata
     5281 gagtttgaga tcttatcagc actctttaaa tctatatttt catcgctact aacgattttt
     5341 tgcaaggtca ttttgttgta agtaatagtt cctgttttat ctgtgcataa aactgtagct
     5401 gaacctaaac tttcaattgc ctgcaatctt cttactaaga catttttgcg tgacatcctc
     5461 catgcaccta aagccataaa aacagttaaa acaacaggaa attcttccgg caaaacagac
     5521 atagctagag tgatacctgt taataaagca ttaactaagt gctttctagt aatataaaaa
     5581 acgataacta cgaccacaca gataagcaaa gcaaaagcgg caaaatattt cactaattta
     5641 cctgtctcct tacttaagcg agtttcctct tccgtgattg cttccaggga tttaccaatt
     5701 tttcccagtt ccgttttttc tcctgtggca aaaacttgag ctatgcctct accactaaca
     5761 accaaagtgc cggaataaac gaagggtaaa tcatcacctc ctggattaat tggtctttct
     5821 tctttattcc aaatccgttt tctaaccggt actgattcac ctgttaaaag cgattcatca
     5881 acacttagtg aattattttc caatatgtag gcatcggctg gaatgcgatc tccttccgct
     5941 aatataatta aatcatcagt tactacttct ctgcctggaa ttctgatttg ttctgaattc
     6001 cgaataacta aagcgcgcgg actggagaga tttttcaatg cctccaaagc tttttctgtt
     6061 ttgcgttcct gaaaaagttc tatagacatt acaataacaa cggatgccgt taggacaagc
     6121 gcttcttgta aatcccctaa aaagaagtag attacagcac aggacaccaa tagaatgaac
     6181 attggttcac taattacatc ccacattaac ttgaatatac tgcgcgcatt ggaacttggt
     6241 aattcattat acccttcccg cttcaattta tcttctactt cttgggtggt caaaccctgt
     6301 ttcaagttac tatattcctt cattttatct cctcaaacaa aataaaatct tggtatcagc
     6361 aattatgttt ttagtcaaga aaaaccggtt cgtgtttagg gaagaaaaga gaaaacggga
     6421 aatattttta ttagcactct cacttttagg cattcccaca aaaaaacgat gtggataagt
     6481 tataacacac tgttgtatag atatatatgt tgtgtaagtg tccaagggat tcgttttgtc
     6541 attcgtggca agggtgatcg agc
//
LOCUS       DAOSTH010000085         6430 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_11091_length_6430_cov_14.025412, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000085 DAOSTH010000000
VERSION     DAOSTH010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6430
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11091_length_6430_cov_14.025412"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(160..567)
                     /gene="mce"
                     /locus_tag="PLB65_07375"
     CDS             complement(160..567)
                     /gene="mce"
                     /locus_tag="PLB65_07375"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HOQ78383.1"
                     /translation="MIKHISHIGIAVKDLSAGITFYENLGLKLEGTEEVPSQKVKVAF
                     FPCGDTRIELLCPTSDDSPIAKFIEKKGEGIQHIAFAVDDLPLALKEANELGINLIDK
                     EPRPGAHHSDIAFLHPKSSLGVLIEFCQEHKED"
     gene            complement(790..1326)
                     /locus_tag="PLB65_07380"
     CDS             complement(790..1326)
                     /locus_tag="PLB65_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HOQ78384.1"
                     /translation="MTKTYEVRLSGSGGQGLILAGIILARAAVLENRKVTQTQSYGPE
                     SRGGYSHADVIISDRDIHFPEATNISCLLALTQEACDKFLFDLSDNGILIIDTTFVKN
                     LALASENTYEVPFTDIAQEKLGSPISTNIMALAFLVKVTGIVKESSLKKAIEESVKPA
                     FIDVNLKAMQIGFDLAKK"
     gene            complement(1326..2165)
                     /locus_tag="PLB65_07385"
     CDS             complement(1326..2165)
                     /locus_tag="PLB65_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOQ78385.1"
                     /translation="MANIPQRIIHEYLRHDKKFPHVWCAGCSNGIVLGAIIRAIASLN
                     IDRNDIVMVSGIGCSSRMPVYVDFNTLHTLHGRSIAFATGVKLHKPHLKVIVVTGDGD
                     CAAIGGNHLIHAARRNIDLKVIMINNNIYGMTGGQCSPTTPHDAYATTAPYGNIEPDF
                     NICNLAMGAGASFVARTTAFHVQEMQSMIKDALQHTGFSLIEVISACPVIYGRLNKKG
                     GAPQMLKDFRDNSIPLSAVEKLPPDKIEGKIIRGILRKEIRPEFTAEYTALCKKIQTA
                     GGE"
     gene            complement(2167..3396)
                     /locus_tag="PLB65_07390"
     CDS             complement(2167..3396)
                     /locus_tag="PLB65_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOQ78386.1"
                     /translation="MTEKDKTKTTKEKASLAAKPESKLEEIQTKRERKTVLMQGNEAV
                     AYGALDAGVDFFAGYPITPSTEIAEILAAELPKRNGAFIQMEDELASICAIIGASLAG
                     AKALTATSGPGFSLMQEGIGFAKITETPCVVVDVQRVGPSTGMPTSPAQGDLMQSKWG
                     SHGDSPAIVLYPDSVKESYELTIRAVNLSEKYRTCVILLLDEVIAHMREAVCLPDIAN
                     VRIINRIKPTVPPQWYKHYDENQKYLSPLASFGEGYRYHVTGLTHDSHGFPTNKPSEA
                     AEMMERLRKKISYNIRDLVQIESYEMEDAKIAIFAAGITSRAAKAAIATARAEGIKVG
                     LLRPLTIWPFPDDAVRKMLRNVDTVIVPELNQGQLIHEVQRLTKDKSDGGLIPINKVS
                     GELITPNDILNKIKEVC"
     gene            complement(3408..3707)
                     /locus_tag="PLB65_07395"
     CDS             complement(3408..3707)
                     /locus_tag="PLB65_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOQ78387.1"
                     /translation="MSKKVEKDRMGMIIREEDTPEKMEVEITEIKAPCDEDSPVLIYY
                     NWCKKCGICVAFCPTGCLGRKSDGSPFVQAPEKCIHCENCDRLCPDFAITGAKDR"
     gene            complement(3727..4941)
                     /locus_tag="PLB65_07400"
     CDS             complement(3727..4941)
                     /locus_tag="PLB65_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOQ78388.1"
                     /translation="MITDLQNILSTNIKGMKRSAIRELLKYLGQPGLISFSGGFPSPL
                     TFPIEELKTIIAEVMENEAAYALQYGTTEGDNLLRTMLANRYKANGIDVTQDNIIITT
                     ASQQALDLISKMFINPGDYVVVGLPSYLGALQAFGSYGANMVGIPMDDEGEDPNILEE
                     TLKDLTAKGKKPKFIYLIPDFQNPAGITMTERRRKQILEIAHKYDVLILEDSPYRELR
                     YEGETQRTLYELDGTGQVVILGTFSKIFCPGFRIGWVVGHPDILDKIVVGKQATDLCT
                     PPFTQRIAARYMEKGYLDSKIADIIEMYSVKKTIMLESLEKYMPEEMKWTHPEGGLFL
                     MVTGPEDLDTNAILLDCIKEANVAYVAGNSFFCNGKGYNTMRLNFSYESIETNIEGCK
                     RLGNYFKKVLQK"
     gene            complement(4964..5254)
                     /locus_tag="PLB65_07405"
     CDS             complement(4964..5254)
                     /locus_tag="PLB65_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78389.1"
                     /translation="MNIHFRDVQSGSVEARAIIEIADGVFLNEVTILNIEGELVVEFP
                     RKSFIGKSRRTHYIDIVTFEDNDKRILWEIEIKNAYKEWRKSNKKVLIYEEK"
     gene            complement(5515..6015)
                     /locus_tag="PLB65_07410"
     CDS             complement(5515..6015)
                     /locus_tag="PLB65_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78390.1"
                     /translation="MKRLIILLMLIAGSFALSAQTGLFNLSYAIPIEEADSLLALSNF
                     YTTNIDESLVRYYPKDSDLVEAILVFLEPQTRYVIGWFIKYNPDNTEENDAFVLQALQ
                     DLHGKTNHYDEETGQLIWFLSTTRTVHIMYADDNSLTVLYYDSYFAEIFRIQDQTLTP
                     ENPESD"
BASE COUNT         1888 a         1316 c         1187 g         2039 t
ORIGIN      
        1 ggcaagtatt atgtacctgc ggtaataatg agacttctgt gctgtaaaaa agttatcaca
       61 aggttcaagg ttattaaggc actcaagatt cccaacttag catttctttc aaatttatat
      121 ctaatctttt aactctttct tttttggtta cttcacccat taatcctctt tatgctcctg
      181 gcaaaattct atcaaaacac ctaggcttga ttttggatgt aaaaaggcaa tatcggaatg
      241 atgagctccc ggacggggtt ctttatcaat cagattgatt cctaattcat ttgcttcttt
      301 tagggcaagg ggaagatcgt caacggcaaa agcaatatgc tgaattcctt cgcctttctt
      361 ttcaataaat ttggctatag ggctatcatc tgaagtaggg cagaggagtt ctattctcgt
      421 atccccacaa gggaaaaaag ccacttttac tttttgcgaa ggaacctctt ctgtcccttc
      481 taattttaag ccgagatttt cataaaaggt gatacctgct gaaaggtctt taactgcaat
      541 accaatatgg ctgatgtgtt tgatcatttt tttattctcc tttatctata ggcgggttga
      601 actcttggtt tagccccaca aacataattt aaggctataa tagaatatag cggaaacaac
      661 attatttggt tgtatgagga acggcaggat gtcctaattc caaatagtta cgagtcctca
      721 ctgccaaatg gattggtggt aagtgtagtt tttaccttgg cagtgatgac tcctaacttt
      781 ttttaaggtt tattttttag ccaggtcgaa gccaatttgc attgctttca gattcacatc
      841 aataaatgcg ggcttgaccg attcttcaat agcttttttt aatgaacttt ctttaacgat
      901 tccggtaact ttcaccaaga aggcaagagc cataatattg gtagaaatag ggcttcccaa
      961 tttttcctga gcaatatccg tgaaaggaac ttcgtatgta ttttcggatg ctaaagcaag
     1021 gttttttacg aaagtagtat cgattatcaa gattccatta tcgcttaaat cgaagaggaa
     1081 cttatcgcag gcctcttgag ttaaagctaa gaggcaggaa atattcgttg cttcgggaaa
     1141 atgaatatcc ctatcactaa taataacatc agcatgggaa tatccacctc tggattctgg
     1201 accgtagctt tgagtttgag taacttttct attttccaaa acagctgccc gagccagaat
     1261 tatgcctgct aaaataagac cttgacctcc acttccggaa aggcgaactt catatgtttt
     1321 agtcatcatt cacctccagc ggtttggatt tttttgcaaa gggcagtata ttcagcagta
     1381 aattcgggac ggatttcctt gcgcaaaata ccacggatga ttttgccttc aattttatct
     1441 gggggcaatt tctctactgc agaaaggggg atggaattat cgcgaaagtc cttcaacatt
     1501 tgaggtgctc cacctttttt atttaagcgt ccgtatataa cgggacaggc actaatcact
     1561 tcaattaaag aaaaaccggt atgctgaagt gcatctttta tcatagattg catttcctga
     1621 acatggaaag cagtggttct agcaacaaaa gaagctcctg cacccatagc cagattgcag
     1681 atattgaaat cgggctctat atttccatag ggagctgtgg tagcataagc atcatgaggt
     1741 gtagtgggac tgcactgtcc acccgtcatt ccataaatgt tattatttat cataatcact
     1801 ttcagatcta tatttctgcg cgctgcatga attagatgat ttcctccgat ggctgcacaa
     1861 tcgccatcac cagtaactac gataactttc agatgtggtt tatgtaattt aacccctgtt
     1921 gcaaaagcga tactgcgtcc atgtaaagtg tgcaatgtat taaaatctac atacacaggc
     1981 atccgggaag aacatccgat tcctgatacc ataacaatat cgttgcgatc tatattcaaa
     2041 gaggcaattg ctcgtataat ggctcctaaa acaataccat tactgcatcc tgcacaccac
     2101 acatggggaa attttttatc atgacgcagg tattcatgaa tgatgcgttg gggaatatta
     2161 gccatcttag catacctcct tgattttgtt cagaatatcg ttgggagtaa ttaattcgcc
     2221 actaacctta ttaattggaa ttaatcctcc gtcactttta tcttttgtta agcgttgaac
     2281 ttcgtgaatc agctgtccct gatttagttc tggaacaatc acggtatcta catttcgtaa
     2341 cattttacga acagcatcat ccgggaaggg ccaaatggtt aaaggtctta aaagcccaac
     2401 tttaattcct tctgcacgag cagtggcaat tgcagctttc gcagcgcgag aagtgatccc
     2461 tgcggcaaaa atcgcaattt tggcatcttc catttcatag ctttcgatct gaaccagatc
     2521 gcggatatta taggatatct ttttgcgtaa acgttccatc atctctgctg cttcacttgg
     2581 tttgttagta ggaaagccat gtgaatcatg agttaaaccg gtaacatgat accggtagcc
     2641 ttctccaaaa ctggccagcg gagaaagata tttttgattc tcatcatagt gtttatacca
     2701 ctgaggtgga acagttggtt tgatccgatt gatgattcgg acattggcaa tatcaggaag
     2761 acaaactgct tcacgcatat gagcaatcac ttcatctaat agcaagatta cgcaagttcg
     2821 atatttttca cttaaattta ctgcccgaat ggttaattcg taactttctt taacggaatc
     2881 cgggtaaaga acaatggctg gagaatctcc atgcgaaccc catttggatt gcatcaaatc
     2941 tccctgggcg ggactagtag gcatacctgt acttggtcca acacgttgaa catcaactac
     3001 cacacatggt gtttcggtaa ttttggcaaa acctattcct tcctgcatca aagaaaaacc
     3061 aggtccgctg gttgctgtta aagcttttgc tcctgccaaa gaagcaccaa ttatggcaca
     3121 aatagaggca agctcatctt ccatttgtat aaacgcacca tttcttttag gaagttcagc
     3181 tgccaaaatt tctgcaattt ccgtagaagg cgtgattggg taaccggcga aaaaatctac
     3241 tccggcatcc aacgctccgt aagcaacagc ttcgttacct tgcataagaa ctgttttccg
     3301 ctctcgtttt gtttggattt cttccagttt actttctggt tttgctgcca gggaggcttt
     3361 ttctttggtt gttttagttt tatctttttc tgtcatcata cactccttca acgatcttta
     3421 gcaccggtaa tagcaaaatc agggcaaaga cggtcacaat tttcacaatg aatacatttt
     3481 tccggagctt gaacaaaagg tgagccgtca ctttttctgc ctaaacatcc ggttggacaa
     3541 aaagccacac aaattccaca cttcttgcac cagttataat agatgaggac gggtgaatcc
     3601 tcatcgcaag gagctttaat ttctgttatt tccacttcca ttttttcggg agtgtcttct
     3661 tcgcgtataa tcatgcccat acggtctttt tcgacttttt tggacattct ttcctccata
     3721 atatacttat ttttgtaata cttttttaaa gtaattgcct aaacgtttgc atccctctat
     3781 gttagtttca atgctttcgt agctgaaatt caaacgcatt gtgttgtagc ccttgccatt
     3841 acagaagaaa ctatttcctg ccacataggc aacattagct tctttgatgc aatcgagcaa
     3901 aattgcattg gtatcaagat cttcaggacc tgtaaccatt aggaatagac caccttcggg
     3961 atgggtccat ttcatctctt cgggcatata tttttccaag gattccagca taattgtctt
     4021 ctttacagag tacatttcga taatatcggc aattttagaa tccagataac ctttttccat
     4081 atagcgagca gcaattcttt gagtgaaagg tggagtgcaa agatcagttg cctgctttcc
     4141 aactacgatt ttatctaaaa tatcgggatg accaaccacc caaccaattc tgaaaccggg
     4201 gcagaagatc ttgctgaatg ttcctaaaat tactacttga ccagttccat caagttcata
     4261 aagggttctt tgggtttcgc cctcataacg caattcgcga tagggactat cttccaggat
     4321 aagaacatcg tatttatgag caatttccaa aatttgttta cgccggcgct cagtcattgt
     4381 gattccagca ggattttgga aatctggaat caaatagatg aattttggtt ttttaccttt
     4441 ggctgtaagg tcttttaaag tctcttctag aatattggga tcctcacctt cgtcatccat
     4501 aggaatacca accatatttg ctccgtagga tccaaaagcc tgaagagctc ctaaatagga
     4561 aggtaaacca acaacaacat agtcacctgg attaatgaac attttggata taaggtctaa
     4621 tgcctgttga gaggcagtag tgatgatgat attatcttga gtaacatcaa tgccattggc
     4681 tttataacgg ttagccagca ttgtccttaa tagattatca ccttcagtag ttccatactg
     4741 aagtgcataa gcagcttcgt tttccataac ttcggcaata atggttttca gctcttcaat
     4801 cggaaaagta agcgggctgg gaaaacctcc ggagaatgaa atcaaaccgg gttgaccaag
     4861 atacttaagc agttcacgga tagcggaacg tttcatacct ttaatgttag tcgaaaggat
     4921 gttttgcaga tcggtgatca ttttgatcct ccttgtattt tttttatttt tcttcgtaaa
     4981 ttaacacttt tttgttggat ttgcgccatt ctttgtacgc attttttatc tctatttccc
     5041 acaggatccg tttgtcgtta tcttcaaaag taacaatatc tatataatga gtgcgtctgg
     5101 atttaccgat aaagcttttg cgcggaaatt ctacaactaa ttccccttct atgttcaaaa
     5161 tggtaacttc atttagaaaa acaccatcag cgatttctat tattgctcta gcttccacgc
     5221 ttccactttg gacatctcta aaatgtatat tcattaataa ctcccattct cttcttatac
     5281 tttgaataaa aaatacacaa attggtcaag cgattttttt tagtggctga gtcacaagga
     5341 aaaaagtcct aatgcaatgt tcaaaaatac cgttaagata ttctcccata aatagcttta
     5401 ctacaataat gcttactgca cattacccta atgtgactga aaaggtaata tgaatcgtac
     5461 acctatgctc aattgataat actattgtgc tgtaacatca attgaattac gggcttaatc
     5521 tgattctgga ttttctgggg taagggtttg atcttgaata cgaaagatct cagcaaagta
     5581 agaatcataa tatagcacag ttaaactatt atcatctgca tacattatat gaacagtgcg
     5641 cgttgtggat aaaaaccaaa taagctggcc agtttcttca tcgtaatgat tggttttacc
     5701 atgcaaatct tgtaatgctt gtagtacaaa agcatcattt tcttctgtat tatcgggatt
     5761 atatttgatg aaccaaccga taacatatct ggtttggggc tctaaaaaaa ccaaaattgc
     5821 ttctaccaaa tcactatcct tagggtaata acgcactaaa ctctcgtcta tattagtagt
     5881 ataaaagtta cttaaagcca gcagggaatc tgcttcctca atagggatag catagcttaa
     5941 attaaaaaga ccggtctgag ccgacagggc aaaactgccc gcaataagca ttagcaggat
     6001 aattaatctt ttcattatac agtccattat cattaattta ttccaattta aattataaga
     6061 gcagaaagtc aagcaaaata agctctcata tcgatgttgc ctctcacggg ttacacagat
     6121 gtagttttag aaacgaggat tacaaaagag tatttagcca ccgaagtcac cgaaagaaaa
     6181 actttgaact aagaactcct gcactttgaa ctttgaacta agaactcctg cactttgaac
     6241 taagaatagt tcacgcagat tacgcagatc acgcagattt agttttagaa acgaggatta
     6301 caaaagaata tttagccacc gaagtcaccg aagtcaccga aagaacaact ttgaactaag
     6361 aactcctgca ctttgaactt tgaactaaga actcctgcac tttgaacttt gaactaagaa
     6421 ctcctgcact
//
LOCUS       DAOSTH010000086         6382 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_11194_length_6382_cov_12.091196, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000086 DAOSTH010000000
VERSION     DAOSTH010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11194_length_6382_cov_12.091196"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(66..2432)
                     /gene="cas3"
                     /locus_tag="PLB65_07415"
     CDS             complement(66..2432)
                     /gene="cas3"
                     /locus_tag="PLB65_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HOQ78391.1"
                     /translation="MVDHLAEVASNAKEMIQKLSLNLNTTTKDNLASVAEKIGIMHDL
                     GKASSYFQNYIHGGKQNHLTHHSFISAVITYVNFNSWEELSCFAPFAFKCVQKHHSNL
                     SSFFNGGLDNAALINETLTIYQSVLDNINNDVGLTKLLQKHNISLPDLSYSHFQLLND
                     NLGDFSLTYSPANIDDTIESFLIQNLLFSVLIDADKHNAAGKEYICLEKLNPRLCYSP
                     AKLIAEKNKKQGALNPLRNQFIANIQENLNIELSQKLYTLTAPTGSGKTFACLEFTNC
                     IQKLEDTSRRVIYCLPYTSIIDQNYKEFESVLHSNLSEQATLDYRFIVKHHHLVDYST
                     VTKAEPTYNLDELQKDILSIESWESACVISTFVQLFHSLIGNRNGMIRKLHNIINSII
                     LLDEVQNLPADYYPLLRVLFKVLAERFDTFILSCSATQPYLFPQDTYIELCEKELFSV
                     PDFNRVKLNIDLKEQTLDEFCQNYLELGDSKSILIVMNTKRSALAIYDYLSSKFKSSY
                     QVLCLTTLHIPLCRQKIIEKIGCIIKEEKTKVVLVSTQLIEAGVDISFQKVYRDFGPL
                     DSIIQVAGRCNRHNELGELGGEMFLVNLQKDGKFLSHYVYDKYLLSKTKECLEGYTSL
                     ESRQFTDIVDNYYRVLDVGAKGRSLLEAISILNYDQNIQNQIPIADFKIITDNYATET
                     LYILCDDTSTKAISSIISKLEYIKQNKLTKEELQKTKTALETEYRSLNSYQINISLNE
                     LNAIYDSYKVKKLNDHIYYIENEFVNDFYECTTGFKLYSTAKSSCLAF"
     gene            complement(2474..3253)
                     /gene="cas5"
                     /locus_tag="PLB65_07420"
     CDS             complement(2474..3253)
                     /gene="cas5"
                     /locus_tag="PLB65_07420"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HOQ78392.1"
                     /translation="MKIPDNFNQILELKLTGKFAHFRKFYTNASSLTYIIPPRTTICG
                     LLASIMQIERDGYYDIMSSENLGVAISLVPNIYYHKVFQTLNYAQDATPKIPINDLSA
                     HKQCRLELLKAQGSEDLEWILFLCFDRMKSNVFTELENKIKVKDFGYGLYLGQRQFIA
                     HLELIRTYNQGEFEPISESDYLDSAIERELIEGITSQDCDLIMERMPLEQELIEGKRK
                     SYRQTKRFGNIVIETNGKRIPGKFKDLIELQNIQRTRISFL"
     gene            complement(3266..4027)
                     /locus_tag="PLB65_07425"
     CDS             complement(3266..4027)
                     /locus_tag="PLB65_07425"
                     /inference="COORDINATES: protein motif:HMM:NF016963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HOQ78393.1"
                     /translation="MNLVSDVRLKRYIRDYFEDIKGEEIFISNKAKDAKERNSQIKAI
                     KKGHIDLIDVRLFGAVTAEEDKGGHFTGPVQFNWGYSLHPVEMVDSSTITSSFSGGQG
                     IGKDYRLYYSLIAFSGSINANTAKETNLSETDLQLLDEAMLNSIPLARTRTKIGQYPR
                     LYLRIELKDKDKFLKDLRSMIKVTSQNSNIAQNLRLIRKPEDYELNINPLIEYLNQNR
                     ELISCINYWKDEQISIPEFDKIFIQENWKKPLSLK"
     gene            complement(4030..6147)
                     /locus_tag="PLB65_07430"
     CDS             complement(4030..6147)
                     /locus_tag="PLB65_07430"
                     /inference="COORDINATES: protein motif:HMM:NF021033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM1802 family CRISPR-associated protein"
                     /protein_id="HOQ78394.1"
                     /translation="MIPLYQAIGKIVLKTVNFNSLPEKEQKLLIVKQYCNELNNYKWP
                     KKVVNNVVNNVDNNVDNNVDNNVDNNVDNNVKDDVGRLLELNLDTTKKVCYFSQGTQL
                     IPEKKDELLVFSNYSPNDPSIYAAHISLKPLLQFELYNYVENNFKKIPWKEETRGKEF
                     LNYLKDIQETFYRIENNIISLKYDKLIEDQQKDFPDPENIPDLKNKLNKKKDYNKAFQ
                     KYLKNYLTSTLEEFVTERQAYALKIDGKFLHQTDYAECYIDVLYYYLFDRHYLDNAKQ
                     GYCHICGNVSTLPKGIAIKQKFFGTTNPLYFDKVDGSLTHNAFSMCEKCNQQVLIGIQ
                     YASTKLRTTLLGLKTIVLPEIKFEIDNDEELIDPQKLNMATKLLERIPLDQKRDDINT
                     LNTLLRRLKEFTLLFYNKPSPTSQEFIINGMIKNINLKELIAKTEHLNKMVQDYNLGS
                     LYGYQACLSFEGLRYLILPSKESHPGMDQNDYTVLNKNIISSLGSYIYKRNFQYYDLI
                     CRFADIYNRKNNNLKTENKYFLNITPFLMTLYLDHLIKFNQLEGVSIMEQRNLISKLQ
                     DERITVYFNSHSQIYENNFFAQGLFILGMYIAAIESEQRKKNIKSTLINRLNLRGIPV
                     QKVKSLMAMVDEMGKVWNIFYDSITENYYRECLQGIENSSLSPEEIVFHILSGRAYSN
                     YLGAMYKLEHPDKTEKNQEEQND"
     gene            complement(6144..>6382)
                     /locus_tag="PLB65_07435"
     CDS             complement(6144..>6382)
                     /locus_tag="PLB65_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas6"
                     /protein_id="HOQ78395.1"
                     /translation="ILKNNLKTLSKAFNYWIPDVEKLNVDGWFKPLEVNFHNQPMQCF
                     TGDFTTNFLIPEFLGLGKQSARGFGVVRKRKEEK"
BASE COUNT         2055 a         1043 c         1004 g         2280 t
ORIGIN      
        1 gctcaattca aacatttcat cttttttgcg taagtaactg ccgtaggtat ttaaatggac
       61 ttccatcaga aagcaagaca gcttgattta gctgtagaat atagtttaaa gccagttgtg
      121 cattcgtaaa agtcattaac aaattcgttc tcaatgtaat agatatggtc attaagtttt
      181 tttaccttat acgaatcata aatagcgtta agttcattga gggaaatatt tatttgatat
      241 gagttcaaag aacgatattc tgtttccaga gctgtttttg ttttttgtaa ttcctccttt
      301 gtaagcttat tctgcttaat gtattctaat ttacttatta tagatgaaat tgctttagta
      361 gatgtatcat cacaaagaat atagagcgtc tcagtagcat aattatcggt aatgatttta
      421 aaatcagcaa tgggtatttg attttgaatg ttttgatcat aattaagaat ggagatagct
      481 tctaataagg atcttccttt tgcaccaaca tcaagcactc gataatagtt atctacaata
      541 tcagtaaact gacgactttc taaggaggtg taaccttcaa gacattcttt tgttttagat
      601 agtagatatt tatcatatac atagtggcta agaaacttgc catctttttg tagattgacc
      661 aaaaacattt ctccgccaag ctcacctaat tcattatgtc tattacatct acctgcaacc
      721 tggataatac tatctaaagg gccaaaatca cgatatactt tttgaaaact tatgtccaca
      781 cctgcttcaa tcaattgagt ggaaaccaaa actacttttg tcttttcttc tttaatgata
      841 caaccaattt tttctataat tttctgccta cataaaggga tatgcaaagt agttaaacat
      901 aaaacttgat aagatgattt gaatttagaa gaaagataat catatatagc aagagcagag
      961 cgtttagtgt tcatcacaat taaaatactc ttggaatctc caagctccag gtaattctgg
     1021 caaaactcat ctaaagtttg ttcctttaga tcaatattta gttttacacg attaaaatcg
     1081 ggaactgaga aaagttcttt ctcacataat tctatatatg tatcctgagg aaataaatag
     1141 ggttgagtgg cagaacaaga caatataaaa gtatcaaatc gttccgcaag caccttaaat
     1201 aaaactctta gtaagggata ataatctgca ggcaagtttt ggacttcatc cagaagaatt
     1261 atagaattaa taatattatg cagcttacgg atcatcccat ttctattgcc aattagggaa
     1321 tgaaacaatt gaacaaaagt agaaataacg caagcagatt cccaggattc aatagacaag
     1381 atatcttttt gcaattcatc caaattgtat gttggctcag cttttgtaac tgttgaataa
     1441 tctaccaaat gatgatgttt tactataaat ctgtaatcta aagtagcttg ctcagagagg
     1501 ttagaatgta acacactttc aaattcttta taattttgat ctattataga agtataggga
     1561 aggcaataaa taactcttcg ggaagtatcc tcaagttttt gaatgcaatt agtaaattcc
     1621 aagcaagcaa atgtttttcc acttccggtt ggagcagtta gagtatataa tttctgagag
     1681 agttcaatgt ttagattttc ttggatatta gcaataaatt gatttcttaa gggatttaaa
     1741 gctccctgtt tcttattctt ttctgcaatt aacttagcgg gggaatagca aagtctagga
     1801 ttaagttttt ccagacaaat atattccttc cctgctgcat tgtgtttatc agcatcaata
     1861 agaacagaga ataatagatt ttgaatgaga aatgattcta ttgtatcatc aatattggca
     1921 ggagaataag taagagaaaa atcacccaaa ttatcattca aaagctgaaa atggctataa
     1981 gacaaatccg gcaagctgat attgtgcttt tgtaggagtt ttgttagtcc aacatcgtta
     2041 tttatgttgt ccaatacact ttgatagata gtaagcgtct cattgataag agctgcatta
     2101 tccagtcctc cattaaaaaa agaacttaag ttactatgat gtttttgcac acatttaaag
     2161 gcaaagggag caaaacagga aagttcttcc cacgagttaa aattaacata tgtaataaca
     2221 gcagaaataa agctatgatg tgttaaatga ttttgtttcc caccatgaat ataattttga
     2281 aaataggaag atgctttccc taaatcatgc attataccaa ttttttcagc aacagaagct
     2341 aaattatctt tagttgtagt atttaaattc agggaaagct tttggatcat ctctttagca
     2401 ttagaagcta cttcagctaa atgatctacc aaaagcttct cctgatatcc attttttgca
     2461 ggatgggata taatcataaa aaagatattc tggttctttg gatattttgt aattcaatca
     2521 aatctttgaa cttaccggga attcttttcc cattcgtttc tattactata tttccaaagc
     2581 gcttggtttg acgataactt tttcttttcc cttcaattaa ttcctgttcc agaggcatcc
     2641 tttccataat taaatcacaa tcttgagaag taatgccttc tatcaattct ctttctatag
     2701 cagaatccag atagtcagat tcagatattg gttcaaattc accttggtta taggttctaa
     2761 ttaattccag atgagcaatg aactgtcttt gtcctaaata taaaccataa ccaaaatctt
     2821 ttactttgat cttgttttcc aattctgtaa atacattact tttcatccta tcgaaacaaa
     2881 gaaaaagtat ccattccaga tcttcagaac cctgtgcttt aagaagttcc aaacgacatt
     2941 gtttatgagc agacagatca tttatcggaa ttttgggggt tgcatcttga gcataattca
     3001 atgtctgaaa tactttatga tagtatatat tgggaactaa agatatggca actccaagat
     3061 tttctgaact cataatatca taataaccgt ctctttcgat ttgcataata gaagcaagta
     3121 aaccacagat tgtagttcgg ggcggtatta tgtaagttag agaagatgcg ttagtataaa
     3181 atttacggaa atgcgcaaat ttacctgtta gcttcagctc taaaatctga ttaaaattat
     3241 caggtatttt catatctttc ccccatcatt taagggaaag cggttttttc cagttctctt
     3301 ggataaaaat tttatcaaac tcaggaattg aaatctgttc atctttccag taattaatgc
     3361 aggaaatcaa ttctctgttt tgatttaaat attctattaa ggggttgatg tttaattcat
     3421 aatcttccgg ttttcggata agacgaagat tttgtgctat attagaattc tgagaagtta
     3481 ccttaatcat actgcgtaag tcctttaaaa atttatcttt gtccttcaat tctatccgca
     3541 aatacaaacg tgggtattgt ccaattttag ttctggttct ggcaagagga atggaattaa
     3601 gcattgcctc atctaaaagt tgcaggtctg tttcggaaag atttgtttct tttgcagtat
     3661 tggcatttat acttccggaa aaggcaatta gggaataata taaacgataa tctttaccaa
     3721 taccttgacc acctgaaaaa ctggaagtga tcgttgaact atcaaccatt tcaacaggat
     3781 gtaaggaata accccagtta aattgaaccg gtcctgtaaa atgtccaccc ttatcctctt
     3841 ctgcagttac agctccgaat agacgaacat caatcaaatc aatatgtcct ttcttgattg
     3901 ctttaatttg gctattgcgt tctttagcat ctttggcttt attagaaata aaaatttcct
     3961 cgcctttgat atcctcaaaa taatctctaa tatagcgttt taagcggaca tcggaaacaa
     4021 ggtttatggt taatcatttt gttcctcctg atttttttca gttttatctg gatgttctaa
     4081 tttatacata gcacctaaat aatttgaata agccctgcca cttaaaatgt gaaagacaat
     4141 ttcttccggt gatagagaag agttctctat accttgcaaa cattctctgt aataattttc
     4201 agttatagaa tcataaaata tattccaaac cttacccatt tcatcaacca tagccatcaa
     4261 ggacttaact ttttgaacag gaataccacg cagattcaat ctattaatta aagtgctttt
     4321 gatgttcttt tttctctgtt cggattctat cgcggcaata tacataccca atatgaaaag
     4381 tccttgagca aagaagttgt tttcataaat ttgggaatga gagttaaaat atacagtgat
     4441 tctttcatcc tgcagctttg aaattaaatt acgctgttcc ataattgata ctccttctaa
     4501 ttgattaaat tttattaagt gatctaaata tagggtcata agaaaggggg ttatatttaa
     4561 aaaatactta ttctcggttt tcaaattatt gtttttccga ttataaatat cggcaaacct
     4621 acagataaga tcataatatt gaaagttcct tttatatatg tatgacccta aagaactgat
     4681 aatgttttta ttcaatacag tataatcatt ttgatccata cctggatgag attcttttga
     4741 aggtagaatt aaatatctaa gcccttcaaa agacaaacat gcctgataac catataaaga
     4801 acctaagtta taatcctgaa ccattttatt caagtgttca gttttagcga ttaattcctt
     4861 tagattgatg tttttaatca taccgttaat gataaattct tgtgaagtag ggcttggttt
     4921 gttataaaaa agcaaagtaa attcttttaa ccttctgaga agtgtattaa gagtattaat
     4981 atcatccctt ttttgatcta agggtattcg ctccagaagc tttgtagcca tattaagttt
     5041 ttgaggatca attagttcct catcattatc aatttcaaac ttaatttcgg gtaggactat
     5101 agttttcaaa ccaagtaagg ttgttcttaa ctttgtagat gcatattgaa ttcctattaa
     5161 aacttgctga ttgcattttt cgcacattga aaaagcattg tgagttaaac ttccgtctac
     5221 tttatcaaag taaagagggt tagtagttcc aaaaaatttc tgtttaatag ctatgccttt
     5281 aggtaaggta gatacatttc cacagatatg acaatagccc tgttttgcgt tatccaaata
     5341 atgtctatca aaaaggtaat aatacaaaac atcaatatag cattctgcat aatcagtttg
     5401 atgcaaaaat ttaccatcaa tctttaaagc ataagcttgt ctttctgtaa caaattcttc
     5461 cagcgtagat gtaaggtaat tttttaagta tttttgaaat gctttgttgt agtccttttt
     5521 tttattcagc ttatttttaa gatcaggtat attctcagga tctggaaaat ccttttgttg
     5581 gtcttctata agtttatcat atttaagtga gataatgtta ttttctattc tgtaaaatgt
     5641 ctcttgtata tctttgagat agtttaagaa ctcctttccc cgagtttctt ctttccacgg
     5701 tatttttttg aagttgtttt ctacataatt atatagttca aattgcagta gtggttttaa
     5761 cgatatgtgg gcagcataga tagaaggatc atttggagaa taattagaaa aaaccaagag
     5821 ttcatctttt ttttcaggaa ttaattgagt accctgggaa aaatagcata cctttttagt
     5881 agtatcaagg tttaactcca ataaccttcc aacatcatct tttacattat tgtccacatt
     5941 attgtccaca ttattgtcca cattattgtc cacattattg tccacattat tgaccacatt
     6001 attgaccact tttttcggcc atttataatt gttcagttca ttacaatact gtttcacaat
     6061 caatagtttt tgttcttttt cgggaagcga gttaaaattc actgttttta atactatttt
     6121 ccctatagct tgatataaag gaatcatttt tcctcctttc tttttcttac tacaccaaag
     6181 ccgcgagcac tttgttttcc gagaccaagg aattcaggga taaggaaatt ggttgtaaaa
     6241 tcgccggtaa agcattgcat aggttgatta tggaaattta cttccagggg tttaaaccag
     6301 ccatccacat ttagtttttc cacatctgga atccaatagt taaatgcctt ggaaagggtt
     6361 ttgaggttat ttttcaggat gg
//
LOCUS       DAOSTH010000087         6323 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_11344_length_6323_cov_17.141991, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000087 DAOSTH010000000
VERSION     DAOSTH010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6323
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11344_length_6323_cov_17.141991"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(221..1693)
                     /locus_tag="PLB65_07440"
     CDS             complement(221..1693)
                     /locus_tag="PLB65_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOQ78396.1"
                     /translation="MNRYKQHLLFWAFITTALFANAVPITIFHTNDTHGAYLPQNSKN
                     GKIGGYANLEYYLNAEREKASRSLYLDAGDQQTGSIFASQKYNNAIGGAVIKVFNSLN
                     LDATTYGNHEFDYSLNNTIKLRKLANYPFISTNILDESKKPFGEIPYKIFKLDSLKVG
                     VLGLTLTDLPEKVKRENVAELTILPYKEAIDKYIEEVDKQSDLLILLTHNGFAADSLL
                     ATELDDRVDLIIGGHSHTVISEPCKVNGIYLASTGAYLNYLGAINLEVQNDHITSYES
                     CLIPLFAPENLPETPLNRFVSAMADSLDIETGKVIATIPEDWVPDKFKETAVSKWAAE
                     ALKNEYYDIYKPDLAIINNGGIRKIIPAGAVTLKDMYELFPFNNYIVLFSCYGKDLLT
                     MESLNREIAENKPYDIVQTSSTQWKKKKRLFGLMKPKELYYINGKQVDPDKIYRVVSF
                     DYILGQWDKYLGFKPFDIQETGDLFTEVMIRQLKKQFSHR"
     gene            complement(1752..2474)
                     /gene="kdsB"
                     /locus_tag="PLB65_07445"
     CDS             complement(1752..2474)
                     /gene="kdsB"
                     /locus_tag="PLB65_07445"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOQ78397.1"
                     /translation="MNVIAIIPARFASTRFPGKPLAMLKGKPIIQSVYERVVNSGLFT
                     SVCIATDDLAIIDAVKRFGGRAVLTSDKHQSGSDRIAEALQYFPKAELVLNVQGDEPL
                     IEKEPLQKLISVFEDREVQMASLMTPLQDLEMLTNPNIVKVITDRQNNALYFSRSAIP
                     YNRDNLSDVTYYRHIGVYAYLPDCLKKFVSLKQGKLERIEKLEQLRALENGISIKMVE
                     TSYQGIGIDTPADLATVEKLLS"
     gene            complement(2471..4318)
                     /gene="mnmG"
                     /locus_tag="PLB65_07450"
     CDS             complement(2471..4318)
                     /gene="mnmG"
                     /locus_tag="PLB65_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOQ78398.1"
                     /translation="MQTSNKFDLIVVGAGHSGIEAALAAAKRGLKVALFTIKLEAIGR
                     MSCNPSIGGPAKGHLAREIDALGGELAKSADLSGIHFRMLNRSKGPAVWAPRSQNDRQ
                     QYHLLMREAVEKQQNIHLMEAIIEGIIVEQGKVKGVISQIGCYYYAPKVILATGTFLK
                     GKIHIGKISYSGGRSGEPSAEELSDSLSAMGLKVRRFKTGTPPRIDLRSLNYNILEEQ
                     PGDLNPQGFSYYRDIELKNLISCYITHTTEETHKIIRDNINKSALYSGIIKGIGPRYC
                     PSIEDKIVKFPQRESHHIFIEPEGINTFEGYLNGVSTSLPADIQEKIVHSIPGLEEAR
                     ILRYAYAIEYDFIDPEEIDNSLECKKIKGLYLAGQINGTSGYEEAAAQGLIAGINASL
                     ALETKDPLIFKRSDAYLGVLIDDLVTRGTNEPYRMFTSRAEYRLLLRQDNADERLMPI
                     GYKLGLVEESRWQRFMHTQEIYQRELEYLKQHNCMAKGEIKEPIRYAQLLKRPEISFT
                     DLPKFGYHIPEDVNADIQNRLELEIKYSGYLNRMEEEIKRFKTAENIAIPKELDYFQI
                     PSLAYEAREKLSKIRPKNIGQAMRIPGINYSDSVALMIWLHKNAKKEAK"
     gene            complement(4320..4931)
                     /gene="yihA"
                     /locus_tag="PLB65_07455"
     CDS             complement(4320..4931)
                     /gene="yihA"
                     /locus_tag="PLB65_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOQ78399.1"
                     /translation="MLRFVESFFVKSAIEPSDYPASAYPEFAFAGRSNVGKSTLINLL
                     TNHKGLAKTSSTPGKTRLLNFFLIRYKIDETDSSGFLQFTDLPGYGFAEVSQAEQEKW
                     RRMINRYLEQRNQLRALIVLVDIRHPADSKDVMLLEMLRVRRIEYSIVATKADKIPKT
                     KIDKTVKDLAKGLGLQEEPIFPVSALNNTGMEPLYNWIQDKLS"
     gene            complement(4924..5295)
                     /gene="queD"
                     /locus_tag="PLB65_07460"
     CDS             complement(4924..5295)
                     /gene="queD"
                     /locus_tag="PLB65_07460"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HOQ78400.1"
                     /translation="MFYLNVIDTFSAAHRLCGYQGPCSNLHGHNWKVRVCVKTEHQDE
                     IGMSLDFGFLKSMLSAILKDLDHRFLNEVEELKNINPTSENLARFIYERMDEALIDKP
                     VTIYEVEVCESDRSSVIYKNA"
     gene            complement(5314..6246)
                     /locus_tag="PLB65_07465"
     CDS             complement(5314..6246)
                     /locus_tag="PLB65_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HOQ78401.1"
                     /translation="MIHLFKVSDQHFIPALTMDEAFWIHLENPSPEEIAKVVTKYNLE
                     EDFITDLQDADENARLEWDEGTLLTILRVPVYYKHRSAKISFATAPLGIISTEDKMIT
                     VAFYDNEILTQYLEGKHRPFNITQQSFLLSISLRAAIYFLKFLKEINRRTTLIEDELQ
                     QSMRNKELIRMVRMEKSLVFFNTSLKSNEIILERLQRTRWLLNDPDAEDLVEDVIIEN
                     KQAIEMTNIYSNILSSMMDAFASIISNNLNIVMKFLTSITIILSVPTLISSIYGMNVH
                     LPFQESEYAFAIVMGLSIIISLVLVFIFIHKKYF"
BASE COUNT         1908 a         1268 c         1065 g         2082 t
ORIGIN      
        1 ttaactcaaa ggccgataca gttatattga tggtggtgta agcccctaga aaaaaatttg
       61 aataaagata taagccctgc tgggacttaa actataaatc ctctaaatcc tataatccta
      121 aaatccttgt ttcgataaat cagatctgcg tcatcagcgt aatctgcgtg aactaatcct
      181 aaaatccttg tttcgataaa tcagatctgc gtcatctgtg ttatctgtga gagaactgtt
      241 tttttaactg tctgatcatc acttcggtaa acagatctcc cgtttcctga atatcaaatg
      301 gcttaaatcc caaatattta tcccattgtc ctagaatata atcaaagcta accacccggt
      361 aaattttatc cggatcaact tgttttccat ttatataata gagttcttta ggcttcatca
      421 aaccaaaaag gcgttttttc tttttccatt gtgtggaact ggtttgcact atatcatagg
      481 gtttattttc agcaatttct ctatttaggg attccatagt taatagatct tttccgtaac
      541 aggaaaacag aactatataa ttattgaagg ggaaaagttc atacatatct tttagggtaa
      601 cagctcccgc gggaataatt ttgcgaatgc caccattatt aataatagcc aaatcgggtt
      661 tataaatatc gtaatattca ttttttaggg cttcggcagc ccatttggag accgctgttt
      721 ccttaaattt atcaggaacc cagtcctcag gaatggtcgc gattactttt cctgtttcta
      781 tatccaagct atctgccatt gcgctaacaa atctatttaa tggtgtttcc ggtaaatttt
      841 ccggtgcaaa aagtggaata agacagcttt cataagaagt gatgtggtca ttttgtactt
      901 cgagattaat tgctcccaaa taatttaaat aagcacccgt agaggcaaga taaattccat
      961 tcactttaca gggctcggaa ataactgtat gggaatgacc tccaataatt aaatccacgc
     1021 ggtcatccaa ttctgtggcc aaaagcgaat ccgcggcaaa gccgttatgg gtaagtaaaa
     1081 taagtaaatc actctgttta tcaacttctt caatatattt atctattgct tctttgtagg
     1141 gaaggatagt taattcggct acattttctc ttttcacttt ttccggcagg tctgttaaag
     1201 ttaagcccaa aacacctact tttagagaat ccagcttaaa tattttgtag gggatttctc
     1261 caaagggctt tttactctca tccagtatat tagtgctgat aaaaggataa tttgccaatt
     1321 tccgtaattt aattgtatta tttaacgaat agtcaaactc gtggttaccg taggttgtgg
     1381 catccaaatt tagagaatta aagaccttta taactgctcc gccaatggca ttattatatt
     1441 tctgcgaggc aaatatgctg ccggtttgtt gatctccggc atctaaatat aaagatcggc
     1501 tggccttttc tctttctgcg ttcagataat attccagatt ggcataaccg ccaattttgc
     1561 cattttttga attttgcggt agataggcac catgcgtgtc gttagtgtga aaaatggtta
     1621 ttggaaccgc attggcaaat aaggcagtag ttatgaaagc ccaaaaaagt aagtgttgct
     1681 tgtatctatt catagtattc tccattgttg gtcccttgaa aatctatata ggttaaaatt
     1741 atattgtagc gttaagaaag cagtttttct accgttgcca aatcggcagg tgtatcaatt
     1801 ccgatgcctt gatagctcgt ttctaccatt tttatcgaaa tcccattttc cagagctcta
     1861 agctgttcca atttttcaat tctttccagt tttccttgtt ttaaagatac aaatttcttt
     1921 aagcaatcag gaagataagc atatacccca atatgacgat agtatgttac atcagatagg
     1981 ttatcccgat tataaggaat agcagaacgg gaaaaataca gagcgttatt ttgcctatcc
     2041 gttataactt tcacaatatt gggattggtt aacatttcca aatcctgtaa aggggtcatc
     2101 aaactggcca tttgcacttc tctatcttca aacacggaaa ttaacttttg cagaggctct
     2161 ttttcaatta agggttcatc accctgaaca ttcaatacca attctgcttt aggaaaatat
     2221 tgtaatgctt cagctatacg atcagaacca ctttgatgct tatcggaagt taaaactgct
     2281 ctacctccaa atctttttac tgcgtctatg atagctaaat cgtctgtagc tatacaaaca
     2341 gaagtaaata aaccggaatt cactactctt tcataaacgg attgaataat aggtttccct
     2401 ttcagcatcg ctaagggctt ccctggaaaa cgagttgagg caaaacgagc tggaataatg
     2461 gcaattacat tcattttgct tcctttttag catttttatg tagccatatc attaaggcaa
     2521 cgctatcact ataattaatt ccaggaatgc gcattgcttg gccaatattt ttaggacgga
     2581 ttttacttaa tttttcgcgg gcttcgtagg caagggaagg aatctggaaa taatccagtt
     2641 ctttaggtat ggcaatattt tcagctgttt taaaccgctt aatttcttct tccatccgat
     2701 ttaaatatcc tgaatattta atttccagtt caagacgatt ttggatatcg gcatttacat
     2761 cttcaggtat atgataaccg aatttaggaa gatcggtaaa tgaaatttcc ggccgtttta
     2821 ataattgcgc atagcggatc ggttctttta tttctccctt tgccatacag ttatgctgtt
     2881 tcaaatattc cagttccctt tgataaattt cctgggtatg cataaatctt tgccaacggc
     2941 tttcttccac taaacccagt ttgtaaccaa taggcatcaa acgctcatct gcattatcct
     3001 gtcttaaaag taaccgatat tcagctcgcg atgtaaacat tcggtagggt tcattagttc
     3061 ctctggtaac aagatcatca atcaaaacac ctaggtaagc atcactccgc ttaaatataa
     3121 gtgggtcttt agtttctaaa gccaaagacg catttatgcc tgctataaga ccttgggctg
     3181 ctgcttcttc atatccagaa gtgccattaa tttgtcctgc caaatataaa ccctttattt
     3241 ttttacattc cagcgaatta tctatttctt cgggatcgat aaaatcatac tcgatggcat
     3301 aagcatatcg taaaatacgc gcctcttcta aaccaggaat actgtgcaca attttctcct
     3361 gaatatctgc tggaagcgaa gtagaaaccc cattcagata accctcaaaa gtgttaatac
     3421 cttccggttc aataaaaata tgatggcttt cacgctgcgg gaattttact attttatcct
     3481 ctatcgaagg acagtaacgc ggtccaatac ctttaataat gccagaataa agagctgatt
     3541 tattaatatt atctctaata attttatgcg tttcttccgt agtatgcgtt atatagcaag
     3601 aaataagatt ctttagttca atatccctgt aataagaaaa accctgagga ttaagatcac
     3661 ctggttgttc ttctaagata ttataattta atgatcgcag gtctatccgg ggaggggtac
     3721 cagttttaaa tctgcgaact tttaatccca ttgctgatag tgaatcggaa agctcttcgg
     3781 cagaaggttc tccactccgt cctcccgaat aactgatttt gcctatatgt attttcccct
     3841 tcagaaaagt tcccgttgcc agaataacct tgggagcata atagtaacaa ccgatttgac
     3901 tgattactcc cttaactttt ccctgctcta caattattcc ctcaataata gcttccatta
     3961 aatggatatt ctgttgtttt tcaactgcct cgcgcataag tagatgatat tgttgacggt
     4021 cattctgaga acgcggagcc caaactgccg gacccttact tctatttaac attctaaaat
     4081 ggattccaga aaggtctgca ctctttgcta attcaccacc taaagcatca atttctcttg
     4141 ccaaatgacc tttagcggga ccgccaatag atggattaca gctcattctg cctatagctt
     4201 ctagtttaat tgtaaacaga gctactttta agcctctttt ggcagctgct aaagctgctt
     4261 caattccaga atgacctgca cccacaacga tgagatcaaa cttattgctg gtttgcatct
     4321 tagcttagtt tatcctgaat ccagttataa agcggttcca tacctgtatt attaagagcc
     4381 gagacaggaa aaatcggctc ttcttgtaat cctaaacctt ttgccaggtc tttaactgtc
     4441 ttgtcaattt tggttttggg aatcttatct gctttggttg caacaattga gtattctatt
     4501 cttcttaccc gtaacatttc taatagcata acatctttgc tatcagcagg atgacggata
     4561 tccacgagca caattaaggc acgcaattga ttacgctgct caagatatct attaatcatc
     4621 cttctccatt tttcttgttc tgcttggcta acttccgcaa aaccataacc tggtaaatcc
     4681 gtaaattgta aaaaaccaga actatcagtt tcgtctattt tataccgaat aagaaaaaaa
     4741 ttaagcaaac gcgtttttcc tggagtggaa gaggtcttag caaggccttt atgattagtt
     4801 agtaaattaa taagtgtaga tttacctaca ttgcttctac ccgcgaaagc aaattccgga
     4861 taggcagaag ctggataatc acttggctca atggcacttt ttacaaagaa agattctaca
     4921 aatctaagca tttttgtata tcacactgct gcggtcagat tcgcagacct caacttcata
     4981 gatagttact ggtttatcaa ttagagcttc gtccattcgc tcatagatga atctagccaa
     5041 gttttcagaa gtaggattga tattttttag ctcttctact tcgttcaaaa aacggtggtc
     5101 aaggtctttc aatatagccg ataacatact ctttagaaaa ccaaaatcaa gagacattcc
     5161 aatttcatct tgatgttctg ttttcacgca aacccgaacc ttccaattgt gaccatgcaa
     5221 attactgcaa ggaccttgat aaccgcaaag acgatgggct gcagaaaatg tatctatcac
     5281 atttaagtaa aacattgctc ctctcccgga ttattaaaag tacttcttat gaataaagat
     5341 aaacaccaaa actaacgata ttataattga tagtcccatc acaatagcaa aagcatactc
     5401 gctttcctga aaagggagat gtacattcat cccataaata ctggaaataa gagtaggaac
     5461 ggaaagaata atcgttatcg aagtaagaaa tttcatcaca atgttcaaat tattggaaat
     5521 gatagaagca aaagcatcca tcatagaact taggatgttg ctgtaaatat tagtcatctc
     5581 tattgcctgt ttgttttcta tgattacatc ttctaccaaa tcttcagcat caggatcgtt
     5641 taataaccag cgcgttcttt gcaaacgttc caaaattatc tcgttggatt ttaatgaagt
     5701 attaaagaaa acaagggatt tttccatccg caccatccga attagctctt tgttccgcat
     5761 tgattgttgt agctcatctt cgataagagt agttctacga ttaatctctt ttaagaactt
     5821 taaaaaatag atcgctgcgc gtaaactgat acttaacaaa aaactctgct gagtaatatt
     5881 aaacggacga tgcttacctt ccaaatattg tgttagaatt tcattatcat aaaaagcaac
     5941 cgttatcatt ttatcctctg tggaaattat ccctaaaggc gctgtggcaa aagatatctt
     6001 agccgaacga tgtttataat aaacaggaac gcgtaatata gttaatagag ttccttcatc
     6061 ccattccaaa cgagcattct catccgcatc ctgaagatca gtaataaaat cttcttccaa
     6121 attatatttt gttacgacct tagctatttc ttccggagag gggttctcca gatgtatcca
     6181 aaacgcttca tccattgtta atgcaggaat aaaatgttgg tcacttacct tgaataagtg
     6241 gatcacttta gaacctcctt cccatattta tttagttctg ccattatttt atatcttgtt
     6301 tttatggcaa gtaaaaaaag cca
//
LOCUS       DAOSTH010000088         6270 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_11485_length_6270_cov_16.410298, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000088 DAOSTH010000000
VERSION     DAOSTH010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6270
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11485_length_6270_cov_16.410298"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="PLB65_07470"
     CDS             complement(<1..418)
                     /locus_tag="PLB65_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOQ78402.1"
                     /translation="MANMINLTINNIPVSVEEGSTILQAARKLNIRIPTLCNHDDLCV
                     AGNCRVCVVEQIGNRTLQAACATPVRENMQILTNSMMVMQARKTIIELLLSEHNADCT
                     KCYKNGNCELQDLSNEYRTGNQVYIDLIPLKHYTIDQ"
     gene            complement(440..1603)
                     /locus_tag="PLB65_07475"
     CDS             complement(440..1603)
                     /locus_tag="PLB65_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HOQ78403.1"
                     /translation="METKKLTRVDYIFRPDNTCMDTLKKSLQRDRDEIVLELIDSGLK
                     GRGGAGFLTGLKWKLAKEAPGDIKYVICNADEGEPGTFKDREILDRVPRRVFTGMALC
                     GYVIGAKEGFLYLRAEYKFLAPKLKTIMAEMNEEFKKLGLDFTLNIRYGSGAYVCGEE
                     TALIDSMEGRRGEPRNKPPFPVTEGYLNKPTVVNNVETFVNCSIIMAIGAEEYKRFGT
                     DSSRGFKLFSVSGDCSKEGIYELELGMLLSDFVEEFGDGDTKAVQVGGASGFCVPRKK
                     FNQTIIGFEGIPTGGSMKIFNSSRSMYHVLHNYLEFFEEESCGQCTPCRVGCQQLLKG
                     IEAVKSKQRPPSYLDELVKLAKVMKVAAKCGLGQSVGNAFVSIVDNFREEIVY"
     gene            complement(1640..2104)
                     /gene="nuoE"
                     /locus_tag="PLB65_07480"
     CDS             complement(1640..2104)
                     /gene="nuoE"
                     /locus_tag="PLB65_07480"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF005722.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOQ78404.1"
                     /translation="MANTEMRVKELAEKYGRNREALMPILQELIDERRYVNDEAMVNV
                     ARELDLSAAEVYGTASFYSFIETEEVGKFVIRLCKTIVCDMKGKEDVLRAIENTLKIK
                     VGQTTADKKFTLKTTNCLGWCHKGPTMLINDEVYTELTPGKAIEIIESYMNK"
     gene            complement(2402..3151)
                     /locus_tag="PLB65_07485"
     CDS             complement(2402..3151)
                     /locus_tag="PLB65_07485"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HOQ78405.1"
                     /translation="MKKFTKLSFAMLFFLGAFMLNVSAADWSAIVKKLSDAQLTAMSN
                     QKPLPYAYNALEPYIDARTMEIHYSKHHATYTSNMNKAAKGQAFESLSLLRIFVEVDK
                     YPVAVRNNGGGFYNHYLFWEIMKPQGGGLPSEILQKAIQDAFGSFDAFKSEFSKAALD
                     RFGSGWAWLSVDETGHLFVSSTPNQDNPLMPIVEKRGIPIFCLDVWEHAYYLNYQNRR
                     ADYVQNFWNLVNWQEVEKRYTEALMALGITK"
     gene            3572..>6270
                     /locus_tag="PLB65_07490"
     CDS             3572..>6270
                     /locus_tag="PLB65_07490"
                     /inference="COORDINATES: protein motif:HMM:NF040284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78406.1"
                     /translation="MPLRLQAQCPPIVTPQPAPGVIINGNNVKFCAGDSVILSSSPSA
                     TSGYSWYHNGTLIPDSTRSIFTAKVAGTYKAKAPGCSNYSNTISLTVNPLPTAYITSM
                     PTPPICSGTPITFYLHTNAPEYYWLPPVPTVLMTEDTITLTLFSSTTLKAVAVSNTTP
                     QCSYFTSYWVRVDFPISGGIIASDQTICSGTVPEPFTNVAYPSGGNGTYTYKWAMSNS
                     GPSGPWNFIPGANDSIYAHPTPLTQTTWFVRFAESPPCSEGISNPVMVTVNPIPEVLS
                     PTDAEICSGNTFSLFPVTNDPGATTSYTSVVSSGTVVGNTPAGNGPINDILTIPEGIT
                     TSGIVTYTITPTGSTPTFCVGTPVEVNATVYPLPQITNPPLEQTICAGEATSPINLTF
                     NIAGSSAVWSATTVPDGIISLTPSSGSGTTIPAITIYSINIDPVDVIFTITPSGPNPL
                     NCPGPSTSFTVTVDPAPTVTNSPLSQEICSGATTEPVTLIPNIPGTNFTWTATPDPST
                     LTGYEPSGTNIIPAQTIINPENEAGTVTYHIIPSEVLGSCAASPRDYIITVYPVPEIT
                     STLTSEICSDNLFEYTMTSDVLNSTFTWNRPTIPEITPDSASGMSPVIYEVLHNSTAS
                     SINVIYNLTAIGPAPLFCDSLPTPLQLLVRPLPIVSAGNDTVIHYGTATQLKGNGTGE
                     SPLLPPQWKPTDKINGDPNQWVVNTTNLYTDTVFTLSVEDDKGCENFDNKWVYLTGTP
                     LAVDPSALNNPICEGSSTLLDANASGGSENYTYLWNPPDYLDDSTIATPTASPPTSIT
                     YTVTVNDGYNTANGTINLMVIPTPTAFNVNGGGAYCAGGTGVSIQLDGSTMGVNYTLV
                     HQNSIGEIDTLYETTQTGLDDTLSWDNQTQAGIYTIIASASGCP"
BASE COUNT         1909 a         1376 c         1061 g         1924 t
ORIGIN      
        1 attgatcaat tgtatagtgt ttcaaaggga tcaaatcgat atatacctga ttgccagtac
       61 gatactcatt cgacaaatcc tgaagttcac agttcccatt tttataacac tttgtacagt
      121 cagcattatg ctcggataat aaaagttcga taatagtttt ccgagcttgc atcaccatca
      181 tgctattggt taagatttgc atgttttctc taactggtgt ggcacaagct gcctgaaggg
      241 tacggttgcc aatttgttca actacgcaaa cacggcaatt accagcaaca caaagatcat
      301 cgtgattgca aagagtagga atacgaatat tcaacttgcg tgcagcttgt aaaatagtgg
      361 aaccttcttc cacacttact ggtatattat taatcgtcag atttatcata tttgccataa
      421 tatattcttt ttaaagtttt taataaacga tttcttctct aaaattgtcg actatagata
      481 cgaaggcatt gcccacagat tgacccaagc cacattttgc tgccaccttc attacttttg
      541 caagttttac gagttcgtcg aggtatgagg gtgggcgctg cttactcttt acagcttcta
      601 tccctttgag caattgttga cagccaaccc tgcatggagt gcattgcccg caagattctt
      661 cttcgaaaaa ttcgagataa ttatgcaata catgatacat agaacggctg ctgttaaaaa
      721 ttttcattga accgcctgtt ggtattcctt cgaaaccaat gatggtttga ttgaattttt
      781 tacgtggcac acaaaaacct gatgctccgc ctacttgaac agcttttgta tcaccatctc
      841 caaattcttc cacaaagtcg ctaagcagca tgcccaattc cagctcatat atgccttctt
      901 tggaacaatc gccactcacc gagaataact tgaaacctct cgaactatca gtaccgaatc
      961 tcttatattc ttcggcacct atagccataa taatagaaca attaacgaag gtttctacat
     1021 tattaactac ggtaggttta ttcaaataac cttctgtaac tggaaaagga ggtttattac
     1081 gaggttctcc acggcgtcct tccatcgaat cgattaaggc ggtttcttca ccacaaacat
     1141 aggccccact tccataacga atattcaaag tgaaatcgag cccaagtttc ttaaactctt
     1201 cattcatctc ggccataatc gtctttaatt tcggagcaag gaatttgtat tcggcgcgca
     1261 aatacagaaa tccttccttt gccccaataa catagccaca caaagccatt cctgtaaaaa
     1321 ctctgcgagg aactcggtcg agaatttctc tatctttgaa agtacctggc tcgccttcat
     1381 cagcattgca aatgacgtat tttatatctc caggtgcttc cttagcaagt ttccatttca
     1441 atccggtcaa aaatcctgct cctcccctgc ctttcaaacc agaatctatc aattcgagaa
     1501 caatctcatc acgatcgcgt tgcaaggatt ttttcaaagt gtccatacaa gtattatcgg
     1561 gccgaaaaat ataatcaaca cgtgtcaatt tcttggtttc catatcgtat taagttttct
     1621 tgtcaaaaaa atttcagatt tacttgttca tataactttc tataatttca atggctttcc
     1681 cgggtgttaa ttcggtataa acttcatcat taataagcat ggtcggaccc ttgtgacacc
     1741 atccaaggca attggtagtt ttcaaggtaa atttcttatc ggctgttgtt tgacctactt
     1801 tgattttcaa agtattttca attgcccgca aaacatcttc cttacctttc atatcgcata
     1861 ctatggtttt acatagccgt attacaaatt tcccgacttc ttccgtttct atgaatgaat
     1921 aaaaggaagc cgttccataa acttcagcag ccgataaatc gagttctctt gctacattca
     1981 ccatggcttc atcgttaaca taacgccttt catctatcaa ttcttgtaaa atcggcataa
     2041 gagcttcacg gtttcggccg tatttttcgg ctaattcctt tacacgcatt tctgtatttg
     2101 ccatttatcc tccttatttt aaatgagtta tcactaaatt tcaattattt aagtttccac
     2161 gaaaaaacct attgttagcg tactaacaat aggtcggcaa aattattgtt tttctactta
     2221 aaagaaaata tttttagtaa ggcggacagg atttaaaaac attctaaata agatgaacgc
     2281 attatcaact ccttatatct gtttaagatt tttttgaaat acgtaaactg aacctcctcc
     2341 ctaaaatgat ccattgcaaa ttttactcac gtaagtgagg atgagcgtat ttaaaaacat
     2401 ctcactttgt gataccaaga gccattaatg cttctgtata gcgtttctca acttcctgcc
     2461 aattgacaag gttccaaaag ttctgaacat aatcagcacg tcggttttga taattcaggt
     2521 aataggcatg ttcccaaaca tcgaggcaaa aaattggaat accccttttt tcaactatgg
     2581 gcattaaagg attatcctga ttgggtgtgg acgatacaaa caagtgacca gtttcatcaa
     2641 ccgataacca agcccaaccg ctaccgaaac gatcgagtgc agccttcgaa aattctgact
     2701 tgaacgcatc aaaacttcca aaagcgtcct gaatagcttt ttgcaatatc tcagacggca
     2761 aacccccacc ctgaggcttc attatttccc aaaaaagata atgattgtaa aaaccacctc
     2821 cattattccg gactgccaca gggtatttat ccacttcaac aaaaattctc aacaaagaaa
     2881 gcgattcaaa agcttggccc tttgcggctt tgttcatatt gctggtatag gttgcatgat
     2941 gcttggaata atgaatttcc atagttcgag catcaatata aggctcaagt gcattgtagg
     3001 cataaggtaa tggcttttga ttgctcatgg ccgttagctg tgcatcactt aattttttaa
     3061 caatagctga ccaatcggca gcagaaacat ttaacataaa agctcctaag aaaaagagca
     3121 tggcgaaaga aagctttgtg aattttttca taatgttacc tcatttagta aattaaaccc
     3181 ttatctaaca aaggaatcga caagtttgtt cgaaaaaaga gatgagaatc aaacaataga
     3241 agaaattaca aaatttcttt aagcactaaa atataaatta aagcatattt tcaaaagcta
     3301 tgcaacaaac taaaaggaga agatgtcttt ttgataattt aattttgaac ttcggtggat
     3361 acaagagaaa actttttatt tttggggaga aaaaatttta cttacttctg gcacatataa
     3421 ttagtatata atgatatagt gttgaatgaa ttactgaaat ttttattgaa ttgtttcaaa
     3481 aaccaaaacc atgataacca aaggaataga gttattaatt aagaaatttt caataattac
     3541 tattgcagca ttttattttg catttagtct tttgccttta agacttcaag cacaatgtcc
     3601 acctattgta acacctcagc cagcaccagg agttataatt aacgggaata atgttaaatt
     3661 ttgtgcgggt gattcagtta ttctttcatc gagtccttca gccacaagcg gatattcatg
     3721 gtatcacaat ggaacattaa ttcccgattc gacaagaagt atatttacgg caaaagtagc
     3781 tggtacttat aaggcaaaag cccctggttg cagtaattat tcgaatacta tttctcttac
     3841 cgttaatcct ttacctactg catatattac cagtatgccc actcctccca tttgcagcgg
     3901 aacacctatc actttttacc tacatacgaa tgcacctgag tattactggc taccaccagt
     3961 gccaacagtt ctaatgacag aggatacaat cacgcttacc ttgttctcat caacaaccct
     4021 caaagccgta gctgtttcca ataccacgcc gcaatgttca tattttacaa gttattgggt
     4081 tcgtgttgat tttccaatta gtggaggcat aattgcttca gatcaaacaa tttgttctgg
     4141 tacagtgcca gaaccattta ccaatgtagc atacccttca ggaggcaacg gaacatatac
     4201 atataaatgg gctatgagca attcaggccc ttcagggcct tggaacttca tacctggagc
     4261 caatgacagc atatacgcac atcctacacc tttaactcaa acaacatggt ttgtacgttt
     4321 cgctgaaagc cctccctgtt cagaagggat aagcaatccc gtgatggtta cggttaatcc
     4381 tattccagag gttctttcac ctactgatgc tgagatatgt tctggaaaca ccttctcttt
     4441 atttcctgtg actaatgatc caggagctac gacatcttat acatcagtag taagttcagg
     4501 tacagttgta gggaataccc cagcaggaaa cggaccaata aatgatattc taacaattcc
     4561 tgaaggaata acgacttctg gtatagtaac ttatactatt acgcccactg ggtccactcc
     4621 aacattctgc gtgggaacac ccgtagaagt taatgctaca gtttatcccc ttcctcaaat
     4681 taccaatccg cccttagaac aaaccatttg tgccggcgaa gccacttccc ccatcaatct
     4741 aactttcaac attgctggtt caagcgctgt ctggagtgct actacagttc ctgatggaat
     4801 aatatctttg acaccatcgt ctggctcggg aactactata ccagccatta ctatttacag
     4861 cataaatatt gatcctgttg atgtcatttt caccattact ccttctgggc ccaatccact
     4921 gaactgtcct ggaccttcaa ctagttttac ggttacggta gatcctgctc ccacagtaac
     4981 aaatagtcca ttatctcaag aaatctgttc tggagcaact actgagcccg taactcttat
     5041 accaaatatt cctggcacca actttacatg gacagcaaca cctgatcctt ctacgcttac
     5101 aggatatgaa cctagtggca cgaatatcat tccagctcaa acaataataa atcctgaaaa
     5161 tgaggctgga actgtaacct atcacatcat tcctagtgaa gtactaggaa gttgcgcagc
     5221 ttctcccaga gactatatca ttactgtata tcctgtgcca gaaattacca gtaccttaac
     5281 tagtgaaatt tgtagcgata atttgttcga atacacaatg acaagtgatg tactcaacag
     5341 tacgtttact tggaatcgcc caactatacc cgaaattact cctgatagtg cgtcaggtat
     5401 gagtcctgtt atttatgaag tcctacataa ctctactgct tcaagtataa atgtaatcta
     5461 taatcttacg gcaattggtc ctgctccttt attttgtgac agcttaccta ctccgcttca
     5521 gctacttgta cgacctctac ctatcgtttc cgctggcaat gatacagtca tccattatgg
     5581 cactgcaact cagttaaagg gcaatggaac gggagagtca ccattacttc ctcctcaatg
     5641 gaaacccacc gataaaatta atggagaccc gaatcaatgg gtcgtcaata ccacgaattt
     5701 atatactgat actgtattta ccttgagtgt agaagatgac aaaggatgcg aaaactttga
     5761 taacaaatgg gtttatctta ctggaactcc acttgctgtt gatccttcag cattaaataa
     5821 tcccatttgt gaaggtagca gtactcttct tgatgcgaat gcttcaggtg ggtctgagaa
     5881 ttatacttat ttatggaatc ctccagatta tctcgatgat tcgactattg ccactccaac
     5941 tgcctcgcct cctacttcaa ttacttatac agtaactgtg aatgatggat acaatacagc
     6001 caatggcacg ataaacttga tggtcatacc aactccgact gcattcaatg taaatggagg
     6061 cggagcctat tgtgccggtg gcacaggagt aagtattcaa ttagatggtt caactatggg
     6121 ggtgaactac actttagttc atcaaaactc aataggtgaa atagatacac tttatgaaac
     6181 cactcagacg ggtttggatg atactttatc ctgggacaat caaactcagg caggaatata
     6241 tactataatt gccagtgctt caggatgtcc
//
LOCUS       DAOSTH010000089         6145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_11743_length_6145_cov_12.109688, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000089 DAOSTH010000000
VERSION     DAOSTH010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6145
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11743_length_6145_cov_12.109688"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(118..1779)
                     /locus_tag="PLB65_07495"
     CDS             complement(118..1779)
                     /locus_tag="PLB65_07495"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HOQ78407.1"
                     /translation="MKRALIILVALTLFCPIAKAEDLRLDLIFSNDVHGGIDRSEATF
                     MNPDFPPLLGGGGSAATLIKHIRSLADIERRDNLLLDAGDIFQGRPVGTVTNGKAVVE
                     FMNAIGYDAMTIGNHEFDIPEEKLKETLQLAKFPILSCNLIDKRTNQIPSYAKPYIVV
                     ERLGLKIGILGFTTTDTEKMSFPENIKNVKFLSEKETIDKYVKILRNQEKVDIVIVLG
                     HAGLPYDIVPTYLTRYDAMGNPLFSERNAVWGYDAQEIAREVEGIDIFIGGHMHKGIP
                     TPWIDPYTHTMVIQGYAYGSNLGWITLKIDPKTKSVSGYEVPALREGSMVTLFEDEFI
                     PDEELGQMIAEQVAIAEKGMDEVVGYAGVYLSRINVDAQSLIGNTIVDAMRAEVNADF
                     AFLNLGGVRADIKAGPVTYRDIFEVMPFDNMIVSFRCTGEFLRKIIETRVEGSRHGLI
                     ISGGKVVYSKKRENFDRVTHFEIGGKPLDPKKIYTVATTDFIMQGNAGLTMLIQVPQE
                     DITNHQINLRDAIVHYFQKNSPIMTRIDDRWTRNDNAKPSAEMLQ"
     gene            complement(1785..3212)
                     /locus_tag="PLB65_07500"
     CDS             complement(1785..3212)
                     /locus_tag="PLB65_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78408.1"
                     /translation="MLSHKTIILLTLLIFASVLSAWSITDTYFGNPYSTFDARSFAMG
                     GAGLFNSKGAFGIADNPANLTLMKKTLGVSANTYLNRNEDNRSIPLYNSFDNYIDDSV
                     YSSNINTFDNYAPAGFYAHRFNKWGVGLGAYYKPVSSFDANYYEEVRNNRNTDNDVYP
                     EKIAINEIESEGTLNKTGIVVSGAYGLSDLIDLNLGFEYGILAGDVKREKSIRWTDWA
                     ITQVGTYHLPELTEIEDYELSGDQFKIGASALLSKRFGLAATFTAGTTLDKKGSYYYK
                     RDAYRNTAVDSINTAIKEDYKLPTQIRFGFVYTPRNVIRTEFNMDIEYVAYSEIHKRY
                     DDVVNFYAGVEHQVVNFMPLRLGFQAVNNWYVTTEEGTDENNHPVTLYHASKIVTPMI
                     TGGSSIKLMDNLKIDLGFGYTWREYEAVDMFGDAYYNDKTYTGASTYALWPNSHITLQ
                     DRGWENPDKVRENNISLNAGLNFTW"
     gene            complement(3225..5987)
                     /locus_tag="PLB65_07505"
     CDS             complement(3225..5987)
                     /locus_tag="PLB65_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOQ78409.1"
                     /translation="MKKFVVMLFVLFGAAFLFAKVDLNTASLADLKQLPITEKQAQDI
                     YDYREFVKVFDNIYELKEIPSIDQATLDRLKPLVSVSIYVETDEAAVRREEIRDLLER
                     LDSNEGASEGMADVWEDYLMTPQNVNKMHLDDFMSLPNVSGIDAVAILKRVAQGDTIA
                     DTRDLRNTQGLSYYGYTNLRSYVYYNEPPVKNRLMCDASLLYYTRYFEEGQYDMLHES
                     FLRNDYNASNVVVPHLKTNSYWGYFGLDELDPDLMLKLRMRYGNNYKLGLMHYSAKGQ
                     EDLFNTKAEDFLADSKWYVGYENNDMPGLYNSRLKVYAGNYRATFGEGLVMENTDYYS
                     ARKTGFGFSKRILGITPDLSRTQENSLRGGAVEYTTPLFGVTAFVSVDNKDALTYIDK
                     DGYAVKRDEYGNDIYTDHDINSSYYGRQYYLNDNGQHIYNYEDGTDVQGDKTKVFALI
                     NPTLRYDDDTMLEAEKWFNDEISAGVPYAMNYINLATRKDVLQEKLWGTHLQVNPFLG
                     TKLGFTTYTSIYDDAHFVVPSYNNLLPTVLRDSYYYSKLVKNLNAEISNLYSTQTDSY
                     TRDYRRVIGFDGQTVIGNTSIQGEYAELSVDGDDLKLGDDPKAYLVSAHTLFENLYFI
                     TLFRNFDVDFDNPYSNSFSEHQRFEDTILEKNVYALTNPTIADLYLNSNQSQPERGVY
                     FETRYKFNRYFTIGRSYLDLWERLTDGRRSARFQSELEFRPIYQLGLRLRYKNQVNRY
                     DDDAERGVSKTNEYTLAVRSFLSNRDFLEFEYRYNTVWNPPYTSLTYPAAEGENSMAA
                     AQTLMIGDYIAVNYTHYFNKSLKMQGSFLYWFGHGISHWDWEDLEIDFMGEKGSKAWV
                     AFTSRISPNMYLNLKFKNKTYQDKEINIRNYNLSDDPTLLDDPVYFQRVEHSENTIRL
                     SIDYRY"
BASE COUNT         1882 a         1278 c         1110 g         1875 t
ORIGIN      
        1 cgccctgagg ggcttttatt ggctatataa ttcaatcccc agaatcccag aaatcttgtt
       61 catcccatac ctattctttt tttaatctgc gaaatccgcg aaatctgcgt gagacaatta
      121 ctgtagcatt tctgcagacg gtttagcatt atcgtttctt gtccagcgat catcaattct
      181 ggtcataatc gggctatttt tttggaaata gtgcacaata gcatcccgca aattgatttg
      241 gtgattggtg atatcttctt gagggacttg aatcagcatt gtgagtccgg cattaccttg
      301 cataatgaaa tctgtagttg ccacagtata gattttcttt ggatccagcg gtttgcctcc
      361 aatttcgaaa tgagtaacgc ggtcaaaatt ttcccgtttt ttggaataga caactttgcc
      421 cccggagatg attaatccat gcctggagcc ctccacgcgg gtttcaataa tcttgcgtaa
      481 aaattctccg gtacaacgaa aagaaacgat catattatcg aaaggcataa cttcaaaaat
      541 atcgcgatag gtaacaggcc ctgcttttat atcagctctc actccgccca aatttaagaa
      601 ggcaaaatcg gcattaactt cggcgcgcat agcatccaca atcgtattgc caattaagga
      661 ttgggcatcc acattgattc tggaaagata aaccccggcg tagccaacaa cttcatccat
      721 tcctttttct gcaatggcaa cttgttcagc tatcatttgt cccaattctt catcgggaat
      781 aaattcatcc tcaaaaagag ttaccatact gccttcgcgt agggcaggaa cttcataacc
      841 gctaacagat ttagttttgg gatcaatttt aagagttatc cagcccagat tggaaccgta
      901 agcataacct tggatgacca ttgtatgggt gtaaggatca atccaggggg tgggaattcc
      961 tttatgcata tgcccaccga taaaaatatc tatgccctca acttctctgg ctatttcttg
     1021 agcatcatat ccccaaacgg catttctttc actaaataaa ggattcccca tagcatcata
     1081 acgcgtgaga taggtaggaa cgatatcata aggaagtccg gcatgtccca aaacaataac
     1141 tatatccact ttctcctgat tacggaggat ttttacatac ttatctatgg tttccttttc
     1201 ggaaaggaat tttacatttt tgatgttttc cgggaagctc attttttcgg tatcggtagt
     1261 agtaaaacct aaaatgccga ttttgagccc taagcgttct accacaatat agggcttggc
     1321 atagctggga atttgattgg ttcttttatc aatcaggttg caggaaagaa tcggaaattt
     1381 agctaattgt aaggtctctt tcagtttctc ttctggaata tcaaattcgt gattgccaat
     1441 ggtcattgca tcgtaaccga tagcattcat aaattccaca actgcctttc cgttggttac
     1501 agttcctacg ggacgaccct gaaaaatatc tcccgcatcc aaaagtaagt tatctcttct
     1561 ttcaatatct gccaaagaac gaatatgctt aattaaagta gctgcggaac ctcctcctcc
     1621 cagcagaggt ggaaaatctg gattcataaa agttgcctcg gaacggtcta tgccaccatg
     1681 tacatcattg gaaaatataa ggtctaaacg cagatcttcc gctttggcga tagggcaaaa
     1741 taaagttaag gcaacaagta taattaaagc tcgtttcata gttattacca ggtaaaatta
     1801 aggccggcat taagggagat attattctca cgcactttat caggattttc ccaaccgcga
     1861 tcttgtaaag taatatgaga atttggccat aaggcataag tggaagcgcc ggtataggtt
     1921 ttatcgttat aataggcatc accaaacata tccactgctt catattcacg ccaagtatag
     1981 ccaaaaccga gatcaatttt caaattatcc atcagtttga tactgctgcc accggtaatc
     2041 ataggggtaa caattttgct ggcgtgatat aaagtaacag gatggttatt ttcatccgtg
     2101 ccttcttcag ttgtgacata ccagttatta actgcctgaa aaccaagccg taaaggcata
     2161 aaatttacta cctgatgttc aaccccagca taaaaattga ccacatcatc atagcgttta
     2221 tgaatttcgc tgtaggcaac atattctata tccatattga attcggtgcg aataacatta
     2281 cgaggcgtat aaacaaagcc gaaacgaatt tgagtgggaa gtttgtaatc ctctttgata
     2341 gctgtattga tactatctac ggcagtattc cgataggcat ctcttttgta ataatagcta
     2401 ccttttttat ctaaagtggt tccggcagtg aaagttgccg ccaaaccaaa acgcttactt
     2461 aaaagagctg aagcgccaat tttaaattgg tctccactta attcataatc ttcaatctca
     2521 gttaattccg gtagatgata tgttccaacc tgagttatcg cccaatcggt ccagcgaatg
     2581 gatttttcgc gttttacatc tccagctaaa ataccatatt caaaacctaa attcaggtct
     2641 atcagatcgc ttaaaccata agcgccagaa acaactatgc cagttttatt gagggtgcct
     2701 tcactttcta tttcgttaat ggcaattttt tcaggataaa catcattatc ggtattgcga
     2761 ttgttccgaa cctcttcata ataattggca tcaaaactgg aaaccggttt ataataggca
     2821 cccaaaccaa cgccccactt attaaaacga tgagcataaa aaccagctgg tgcataatta
     2881 tcaaaagtat tgatattgga ggaatagaca gaatcgtcaa tatagttatc aaaagaatta
     2941 tacaagggta tactgcgatt atcttcattg cgattcagat aagtatttgc tgaaactcct
     3001 aaggtctttt tcatcaaagt tagattggca ggattatcgg ctattccgaa agcacccttg
     3061 ctattaaata aaccagctcc tcccatagca aaactgcggg catcaaaagt ggaataagga
     3121 ttgccgaaat aggtatcggt aatgctccag gcagaaagaa ctgaagcaaa tattaagaga
     3181 gttaaaagta tgattgtttt atgagataac attattcctc cacattagta acggtagtca
     3241 atactcaagc gaattgtatt ttcgctatgt tcaactcttt gaaaataaac cggatcatcc
     3301 aatagggttg gatcatcgga gagattataa ttacggatat ttatttcttt atcctgatag
     3361 gttttatttt taaacttcag gttcagatac atatttgggg aaatgcgact ggtgaaggca
     3421 acccatgcct tggaaccttt ttcacccata aaatcaattt ccagatcttc ccaatcccaa
     3481 tgcgaaattc catgcccaaa ccaatatagg aaagaacctt gcatctttaa tgatttatta
     3541 aaatagtgag tataattaac tgctatatag tccccaatca tcaaggtctg agctgctgcc
     3601 atagaatttt cgccttccgc ggctggataa gttaaagatg tataaggggg attccaaacc
     3661 gtgttataac gatattcaaa ttccaaaaag tcacgattgg agaggaaaga ccgaactgct
     3721 aatgtatatt cgttagtttt ggaaacaccg cgttctgcat catcgtcata acgattaacc
     3781 tggtttttat aacgcaatcg caaacccagt tgatagatag ggcgaaattc cagttcactt
     3841 tgaaatctgg cacttctgcg tccgtcagtt aatcgttccc agaggtctaa atagcttctg
     3901 ccaattgtga aatagcgatt gaacttgtag cgggtttcaa aatatacacc gcgttcaggt
     3961 tgtgactgat ttgagttaag atataggtct gcaatagtgg gattggttaa agcataaaca
     4021 tttttctcta aaattgtatc ttcaaaacgc tgatgttcag agaaactatt actgtaagga
     4081 ttatcgaaat ccacatcaaa attgcggaac aaagtaatga aatagaggtt ttcaaacagc
     4141 gtatgcgcag atacaagata tgcctttggg tcatctccca atttgagatc atcgccgtcc
     4201 acggaaagtt ctgcatattc accctggatg gaagtgttgc caattacggt ttgaccatcg
     4261 aaaccaatca ctcttctgta atctcgtgtg tagctatccg tttgagtgct atagagatta
     4321 ctgatttccg cattcaaatt ttttaccaac ttagaatagt aataggaatc ccgtaaaaca
     4381 gtaggtaaca aattattata actaggaact acaaaatggg catcgtcgta aatggatgta
     4441 taggttgtaa aaccaagctt tgtgcccaaa aatggattta cctgcaaatg cgttccccac
     4501 aatttttcct gcaaaacatc tttgcgggtg gcaagattaa tatagttcat tgcataaggg
     4561 acgcctgcag aaatttcatc attaaaccat ttctctgctt ccagcattgt gtcatcatca
     4621 taacgcaaag taggattaat aagtgcgaaa accttggttt tatcaccctg aacatcagtt
     4681 ccatcttcat aattatagat atgctgtccg ttatcattaa gataatattg tctgccataa
     4741 tagctggaat ttatatcatg gtcagtatag atgtcgttgc catattcgtc gcgtttaaca
     4801 gcataaccat ctttatcaat ataagtgaga gcatctttat tgtcaacaga tacaaaagct
     4861 gtaacaccaa acaagggagt agtatattct acagcacccc ctctcagaga attttcttga
     4921 gttctggaaa ggtctggagt gatgcccaaa atccttttgc tgaagccaaa accggttttg
     4981 cgggcactat aataatccgt attttccatc actaaccctt ctccaaaagt ggcacgataa
     5041 tttccggcat aaaccttcaa acgcgagttg tataaaccag gcatatcatt attttcataa
     5101 ccgacatacc atttggaatc ggctaaaaag tcctctgctt ttgtattgaa caaatcttct
     5161 tggccttttg cagaataatg catcaaaccc agcttatagt tatttccata gcgcattcta
     5221 agctttaaca taagatcggg atcaagttca tccaagccaa aataacccca gtaagaattg
     5281 gtttttaagt gcggaaccac aacattggaa gcattatagt cattacgcaa aaaactctca
     5341 tgcagcatat catactgccc ttcttcaaaa tagcgagtat aatataataa cgacgcatca
     5401 cacatcaaac ggttttttac cggcggttca ttgtaatata cataactgcg caagttcgta
     5461 taaccataat aagaaagacc ttgagtattg cgtaaatccc ttgtatcggc aatagtatca
     5521 ccttgagcaa ctcttttcaa aatggcaact gcatcaatgc cagaaacatt gggcaagctc
     5581 ataaaatcat ccaaatgcat tttgttcaca ttttgaggcg tcattaaata atcttcccaa
     5641 acatctgcca tcccttctga ggcaccttca ttactatcca aacgttccag caaatccctg
     5701 atttcttctc tgcgaacggc tgcttcatcg gtttccacat atatagagac tgatactaag
     5761 ggttttagcc gatcgagcgt tgcttgatcg atagaaggga tttcttttag ctcatagata
     5821 ttatcaaaga ccttcacaaa ctcccggtag tcatagatat cctgtgcttg cttttctgtt
     5881 atgggcaatt gctttaaatc tgccaaagaa gctgtgttta aatctacctt ggcaaataaa
     5941 aacgctgcgc caaacagaac gaagagcatt actacaaatt tcttcatcgc ttttccttaa
     6001 agtgctttat actttttcct ttataatata accactaatt gacactctgc aagtatcaat
     6061 aaatctgtcc agttttttct ttgcagttgt tagccattgc gatttgtatt gttttgtttt
     6121 aactctcaat tttgaaatct tgata
//
LOCUS       DAOSTH010000090         6041 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_11993_length_6041_cov_11.595389, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000090 DAOSTH010000000
VERSION     DAOSTH010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11993_length_6041_cov_11.595389"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..592
                     /locus_tag="PLB65_07510"
     CDS             <1..592
                     /locus_tag="PLB65_07510"
                     /inference="COORDINATES: protein motif:HMM:NF021622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78410.1"
                     /translation="STFSKLHYTRKSQSYKNAIDIAQLLFSNLHPDLQAGKNSVLALM
                     FDMNLLWERFIYSSLAKMFRQCNNGYIVTAQYPMPFWNTNNNRKDIKPDIVIQSPEGE
                     TFILDTKWKIGSDNSPDSSDLYQLFAYSQFFQAKRTALIYPSLSKNIVPGKYLMPNLS
                     DGNPYCDLIQLEVQPKISDWQKKIYEEILGWMENQT"
     gene            771..1478
                     /locus_tag="PLB65_07515"
     CDS             771..1478
                     /locus_tag="PLB65_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78411.1"
                     /translation="MKISAIFYAIVILAFLMPFFVVSCDTTELASLKGIKLVTGGEVK
                     ITIPEIMQDMQQDENKINNPSMSAQPFAIVAIVLAVAALILVLALPVKMYLLPAIVSI
                     LGIISLQLLNANIMSVLSSTKTGLDPSIDLSSIMSIKTQSGFWLANIAFILGGVFTII
                     LGMKNQSKLALSPQAVEEVSASIISDENLSDWEGTENKTFSEVDDNNIISSEENETVD
                     QEEEEEEKEKEEKEKLE"
     gene            1488..2546
                     /locus_tag="PLB65_07520"
     CDS             1488..2546
                     /locus_tag="PLB65_07520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOQ78412.1"
                     /translation="MDSLAELRWIRISVIIIAIAVIAYILKTLKVIFIPLVFAVFLLF
                     LFAPLINYLKKHKVPMFLILLITFVIIALFLGVVILLIYAASNSLISGLPRYEDKFNS
                     LLSTITIYIQNFANRWNISSENFSFNNITQILSSGFISLPQLLSNTMNTLVSVIQNIF
                     LIIIFLIFLLLEIDKLPIRLKRVTTKFSKEQTLNIIQNIEKQIQNYLTIRTLINLSAS
                     FLCMLWMYIFGVDFILVCGILLFVLDFIPDLGSVLASAIPILIYLLQSGFSIELLFFS
                     LLIVATQMLIGNLLEPKLQSVQLNLTPIMVLVSLIFWGWLWGIVGMLICVPLTSAINI
                     ILKQVAPDNVISALISSD"
     gene            complement(2901..3758)
                     /locus_tag="PLB65_07525"
     CDS             complement(2901..3758)
                     /locus_tag="PLB65_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78413.1"
                     /translation="MNQCKTLLKKEWWINWKQLLLPVWVSLAFIGVCIIGLLIGLIKG
                     KGITYFRMEGNFPPGFNNLFLFGSVQTLNIALGFLSIMVAIMLADNLINGGFKLKCEI
                     LHQSQPVSLTKILSSKYVFLILGSFIVYAVISFIGTLVITFTQMYFTSALFYFGFYAW
                     LQSAVELFFTLIFVCSLFWFFACLFQRKSFFMGILTLIGIELAIKILNYTSALNIPSL
                     LGYLTKFIKISSDPGFGKAMDLNVNTLISIVQANWKNILSNNTLLKLLYSAILTLGGS
                     LFYKRRELI"
     gene            complement(3745..4602)
                     /locus_tag="PLB65_07530"
     CDS             complement(3745..4602)
                     /locus_tag="PLB65_07530"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ78414.1"
                     /translation="MTEESYIVHNVTKYYGKFKAIDSVSLELRQGKIIGLLGKNGAGK
                     STLMRCMLGFLNHSGEVYIDGNLIKRRDPKVFEKVAFIPDVSGLDDRLTVKQTIDYVK
                     RINPKWNEVTANKLLEISNLPVNKKVSQLSKGMKTKLYLMITLSLDVNYLILDEPTLG
                     LDIAFRKEFFNTILNEFYEGNKTIFISTHQVEEVEDILQEIVFIDKGKILLHEEVHTL
                     KELFKIVSLPLEQSEKIMQYNPKVYTKNMGFMNAVLSSDIEIEGAIYGKANLADIFLA
                     MVGGTNESV"
     gene            4861..5922
                     /gene="dinB"
                     /locus_tag="PLB65_07535"
     CDS             4861..5922
                     /gene="dinB"
                     /locus_tag="PLB65_07535"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOQ78415.1"
                     /translation="MKKIIHIDMDAFFAAIEIRENPRLKGKCVIVGGPPNSRGVVSTC
                     SYEARKFGVHSGMSSYQAWNLCPDAIFVDHHFNLYIQVSNQIREIFYKWTDTVEPMSL
                     DEAYLDVTVNKMGEEDTVKIAQLIKEEVFLKTQLTCSAGVSYNKFLAKIGSDLNKPNG
                     LTFIPPEKAKEVLFALPIEKFHGIGKVTAARMKKMGINNGADLYKRELKDLLMHFGKI
                     GIFYYYVVRGIDNRELITNFEPKTLSCETTFDKDVDNLEELLTVLHHLSERLSERLHR
                     KGIRGNSLTLKLKYDNFQLITRSCRMQNNTYICEDLYKYGEQLLIANWDCSRKVRLLG
                     LGISNLDNVTTNEQLLLPL"
BASE COUNT         2068 a          982 c          997 g         1994 t
ORIGIN      
        1 aagcactttt tcaaaattgc attataccag aaaatcacaa agctataaga atgccattga
       61 tattgctcaa ttactttttt caaacttaca tcctgatctg caagcgggta aaaattctgt
      121 tctggctttg atgtttgata tgaacttatt atgggaaaga tttatttact ccagtttagc
      181 taagatgttt cgtcaatgta ataatggcta tattgttaca gcacaatacc ctatgccttt
      241 ctggaacaca aataacaatc gtaaagatat caaacctgat attgtgattc aaagtccaga
      301 aggtgaaact ttcattttgg atacaaagtg gaaaatcggt tctgataaca gtcctgattc
      361 ttccgattta tatcaattgt ttgcttattc acaattcttc caagctaaac gaactgctct
      421 catttatccc agcttatcta aaaatattgt tccaggtaag tatttaatgc caaatctctc
      481 agatggaaat ccttattgtg atctaattca actggaagtt caacctaaaa tatcagattg
      541 gcaaaagaaa atctatgagg aaattttggg atggatggaa aatcaaacct agcactcata
      601 ataaataaat cttggcgtta taacaataat ttgagtagga tcaaattcat attaaacttg
      661 aagaataaat gaggtttaag gcaaaaacta aacaagataa gcaaagaaat ttgcttgaca
      721 gtaaaaagtg attataatat gaagaaaaaa taaaagaaag ggaggatatg atgaagatat
      781 ccgcaatatt ttatgctatc gtaattcttg cgtttttaat gccatttttc gtcgtcagtt
      841 gtgatacgac agaacttgct tcgttaaaag gtattaaact cgttacaggt ggagaagtta
      901 aaattactat acctgaaatt atgcaagata tgcagcaaga tgaaaacaag attaataatc
      961 cgagtatgag tgctcaaccg tttgccatag tagcaattgt tttagctgtt gctgctttaa
     1021 tattggtatt agcattaccg gtaaaaatgt acttattacc tgcaatagtt agtatcttag
     1081 gtattatttc gctacagttg ctaaatgcga atattatgag tgtgttatct tctaccaaaa
     1141 ccgggctaga cccttcaata gacttaagca gtataatgtc tattaaaacc caatccggtt
     1201 tttggctggc aaatattgct tttattttgg gtggtgtatt tactatcatt ttaggaatga
     1261 aaaaccagag taaattagcc ttaagtccgc aagcagtaga agaagtatca gccagtatta
     1321 tctctgatga gaatttatct gattgggaag gaacggaaaa taagaccttc tctgaagtgg
     1381 atgataataa tattatatct tcagaggaaa atgaaacagt agaccaagaa gaagaagaag
     1441 aagaaaaaga aaaagaagaa aaagaaaaat tggaatagct aaaacttatg gatagtttag
     1501 cagagctaag atggattcgc atttcggtga ttataatagc gatagcagta attgcttata
     1561 tccttaaaac gctaaaagtt atttttatcc cgttagtatt tgctgtcttt ctgctctttt
     1621 tatttgcgcc actgattaac tatttgaaaa agcataaagt tccgatgttt ttaattctgc
     1681 tgatcacttt tgtaattatt gctctttttt tgggagtagt aattctttta atctacgcag
     1741 cttcgaatag tttaatcagt ggtctacctc gttatgaaga taagtttaac tccctactat
     1801 caacaattac tatatatatt cagaattttg ccaatagatg gaatatatct tcggagaatt
     1861 tctcattcaa caacattaca caaatattaa gcagcggatt tatttcgcta ccacaattat
     1921 taagtaacac aatgaataca ttagttagcg taatacagaa tatctttcta attatcatct
     1981 tcttaatctt tttgctgttg gaaattgata aacttcctat acgtttaaaa agggtaacta
     2041 ccaaattcag caaagaacag acattaaata ttatacaaaa catagaaaaa caaattcaga
     2101 attatctcac tatcaggaca ttgataaatt tatctgcctc ttttctatgt atgctatgga
     2161 tgtatatttt tggtgtagat ttcattttag tgtgtggtat tttacttttt gtactggatt
     2221 ttatacccga tttaggttca gttctggctt cagcgattcc tattcttatc tatttgttac
     2281 aaagtggttt tagtatcgag ctattatttt tttcgctgct gattgttgct actcaaatgt
     2341 tgattggaaa tttactggaa cccaagctgc aaagcgtcca attgaattta actccgatta
     2401 tggtattggt ttccttaatt ttttggggtt ggctatgggg aattgtagga atgttgattt
     2461 gcgttccttt aacttctgcc atcaatatta ttttaaaaca ggttgcaccg gataatgtaa
     2521 tttctgcact aatcagcagc gattaggatt tattttatca cctttactat atgaaaatgc
     2581 aattgtatta atttaaccat atacacaata tctccaaaat gaaacttgta tctttcttag
     2641 ttaattgtgg tagaattgag gtgctgttcc cgggcaaaat accgagcaac actaccgcag
     2701 ctctactgta accaactagg taataataaa cataagctac catatttagg ttatataaag
     2761 caatagaata ttcttaatct tctctgaaac ttgataactg aattaatggt tttatgtgct
     2821 tggcatatgc aaaatagctt gcctattcga ctgctgcaga tgtgctatct aattaactgt
     2881 ttgctaagta ctaattgcag ttaaataagc tcacggcgct tgtagaaaag agatcctcct
     2941 aatgtaagaa tagcgctata gagcaatttt aacagagtat tgttacttag tatatttttc
     3001 caattcgctt gaactatgct tataagagta ttaacattca aatccattgc ttttccaaaa
     3061 cctggatcgg aggatatttt aataaatttg gttaggtaac caagcaacga aggaatattc
     3121 aatgcggaag tataattcag aattttaatc gctagttcta ttccgattag cgttaagatt
     3181 cccataaaaa aggacttgcg ttgaaacaaa caggcaaaaa accaaaacag cgagcaaaca
     3241 aaaatcagtg tgaaaaataa ttccacagca ctttggagcc aggcataaaa tccaaaataa
     3301 aacaaagcag aagtaaagta catctgagta aaagtaataa ctaaggtccc gataaagcta
     3361 ataaccgcat aaacgataaa tgatcccaaa attaaaaaga catatttact gctaagtatt
     3421 ttggttaaag aaaccggttg agattgatgt aatatttcgc atttaagttt aaaaccgcca
     3481 ttaatcaggt tgtctgctaa cataatagct accattatgg ataaaaaacc caaagcaata
     3541 ttcagagttt gaacactgcc aaagagaaac aggttattga atccgggggg aaaattacct
     3601 tccattctaa aataggtaat gcctttccct ttgataagac caattaataa gccaataatg
     3661 caaactccaa taaaggcaag cgaaacccat actggtaata acaattgttt ccagtttatc
     3721 caccattctt tctttaacaa ggtcttacac tgattcattt gttcctccca ccatagctaa
     3781 aaaaatatct gcaagattgg ctttcccgta aattgcacct tcaatttcta tatccgaaga
     3841 cagcacagca ttcataaaac ccatattctt agtatacact ttggggttat attgcataat
     3901 tttttcagat tgttccagag gcaaacttac tatcttaaaa agttctttaa gtgtatgaac
     3961 ttcttcgtgc aatagaattt tccctttatc aataaagacg atttcctgta atatatcttc
     4021 cacttcctcc acttgatgag tagagataaa tattgtttta ttcccttcat agaattcatt
     4081 taagatggta ttgaagaatt cttttctaaa ggcgatatct aaccctaagg taggttcgtc
     4141 caatataaga taattaacat caagggaaag agttatcatt aaatacagct tagttttcat
     4201 ccctttggaa agttgagaaa cttttttatt cacaggtaaa ttgcttattt ctagtaactt
     4261 attcgccgta acttcgttcc attttggatt tatccttttc acataatcta ttgtctgttt
     4321 aacagttaat ctatcatcca aaccactcac atccggaatg aaagctactt tttcaaaaac
     4381 tttcgggtct cgccgtttaa ttaaattccc gtctatataa acttcaccac tatgatttag
     4441 aaaacccagc atacaacgca ttaaagttga tttgcccgct ccgtttttac ctaaaaggcc
     4501 aataatttta ccttgtctaa gttcaagtga aacactatca atagctttaa atttaccata
     4561 atatttcgtt acattgtgaa ctatataact ttcttctgtc actgcttacc tctaatatat
     4621 attacttaat ttttagggac tatctgttct aagtgtaacc tatagtaaga tttctgatag
     4681 ttaaaaaaaa gctaacttga ttgctaggta aggatactgt agcttattca tctgtatcag
     4741 aaaagatcta ttatctgtac cattttgtta actaatcact cttttttcca atcttataaa
     4801 gagagtctgc tgtcaagttt tttcttgact taaaggtgat tgtaaaagaa tagaatcatt
     4861 atgaagaaaa taattcatat tgatatggat gccttttttg cggctatcga aatcagagaa
     4921 aatccccgac tgaaagggaa atgtgtaatt gtaggtggac cacctaatag tagaggtgtg
     4981 gtttccactt gttcttatga agctcgtaaa tttggtgttc atagtggaat gagcagttat
     5041 caggcctgga atttatgtcc ggatgcaata tttgtagatc atcattttaa tctatatatt
     5101 caggtatcca atcagataag ggaaatcttt tataaatgga cagatacagt tgaaccgatg
     5161 tcattagatg aggcatattt agatgttaca gtaaataaaa tgggtgaaga agataccgtc
     5221 aaaattgccc aactaatcaa ggaagaggtg tttcttaaaa cacaattaac ttgttctgca
     5281 ggtgtttcat ataataaatt tctcgctaaa attggcagtg atctgaataa accgaatgga
     5341 ttaactttta ttccaccaga aaaagcaaaa gaagttttgt tcgctttacc aatagagaaa
     5401 tttcatggta ttggtaaagt cactgctgcg cgaatgaaaa aaatggggat aaataatggt
     5461 gcagacctct ataaacggga attgaaagat ctactaatgc attttggtaa aataggtata
     5521 ttctattatt atgtggtgcg aggaattgat aatcgagaac taattactaa tttcgaaccc
     5581 aaaaccctaa gctgtgaaac tacttttgat aaagatgttg ataatttaga agagctttta
     5641 actgttctac atcacctgtc ggaacgcctt tcagaacgtt tacatagaaa gggtattaga
     5701 ggaaacagtt taactttgaa gttgaaatac gataatttcc aattaatcac tcgtagctgt
     5761 cggatgcaaa ataatactta catctgcgaa gatttgtata aatacggaga acaactttta
     5821 atagccaatt gggattgttc tcgtaaggtt cgtttattag gcttgggaat aagcaacctt
     5881 gataatgtaa caactaacga acaattatta cttcctcttt aaaacaccct taaaaccctt
     5941 ttgcatcaaa ttactgaaaa caattaataa aagcccgata aaagtagcat aatgaatgct
     6001 ctccttcaaa atagaactga tgaaaagcaa agatacaaaa g
//
LOCUS       DAOSTH010000091         5819 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_12566_length_5819_cov_10.031228, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000091 DAOSTH010000000
VERSION     DAOSTH010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12566_length_5819_cov_10.031228"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            900..1856
                     /locus_tag="PLB65_07540"
     CDS             900..1856
                     /locus_tag="PLB65_07540"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOQ78416.1"
                     /translation="MMINILAVIIFKLDTPFDLILSILGIAFSIIQNPFKIFGSTVFG
                     IPLATVDNVHLTEYAFVLILVVFAYYIFKINIKVSEHNSSEIERLYTNIYRLTKMNVV
                     FQDKAISLKAESASKERNRIIREVHDITGYYFTNILMLLKTTLLLVKQNDNKSVQLLE
                     NAIKQVLQGIDEIRRTLKILNTISSQKIASNVNSLLELTQAFQYLTDIKVELLFGNIA
                     KTYGTTADLTIYRFVQEGLTNAFRHGRATKIEVQFWATTELLTINILDNGMGAFKFNK
                     GMGISGMEERLSRIGGTLEAGNISDGFQISAFIPLPIHEEQE"
     gene            1853..2530
                     /locus_tag="PLB65_07545"
     CDS             1853..2530
                     /locus_tag="PLB65_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOQ78417.1"
                     /translation="MNKTIKVLLADDQKLFIDSLKTAIECLSDDIQIIGIAYNGLEVI
                     DLIHKEIPDIILLDVRMPKMDGVETAKYISVNYPKIKIVMLTTFDDDDYVQEALKYNV
                     VGYILKDTPLTELITMIPIVNEGTVLISQKVAKKLLSGHHHKNMGQSASITDEKLDSM
                     PEWYDELTPRELEILVCVAQGLDNGEIADSLCIAEQTVKNHISIIYSKLGVHKRLQAM
                     RLGSSLL"
     gene            complement(2565..4343)
                     /locus_tag="PLB65_07550"
     CDS             complement(2565..4343)
                     /locus_tag="PLB65_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78418.1"
                     /translation="MCNSTAPNAHYGNRNTQISDSWNANRSDQIVFDYYYARLNMSTI
                     ATEVLYNGIKLDVEWPPRNQNVDTFSAFDAPYLENPPHIIPIDIGRQLFVDDFLIEET
                     DCVRFFVKPKIHPSSPILIPETYEEMDRGNCPMAAPFNDGVWYDSSDQLFKMWYMAGW
                     FHTTAIATSVDGIRWTRPQLDVVPGTNLVWKTQKSYERDGCLVWLDGNASNPEQKFKM
                     FQFYRYNSDVFYDGDSEVSWLQTSKDGIHWSTPVSTTPVGDNTSFFYNPFRKKWCMSI
                     RRAIESNNKLLPIGIRSRFYTESDDFMKGANWDVSSSEVLWHLADTHDLPDPAFPNHV
                     VTLYDVNAVAYESLMIGLFGIFRGPENNICKDLGIPKTIDLELGYSRDGFHFTRPDRT
                     PFIGSSRVVGDWRRAYLHAAGGICLVVNDELYFYFTGFSGESPNLKNDETGSPGRMRN
                     VMYAGASTGLATLRRDGFAFMKPKDEEGRLTTRLLSFSGTHLFINGNFSDGELQVEIL
                     DVNGRIIEGYSRYDCLPVSSDKTKVEIQWKERNSLVNVKKQPIHIRFYLRNGELYSFW
                     ISASETGASNGYVAAGGPGFTGGRDI"
     gene            complement(4253..5095)
                     /locus_tag="PLB65_07555"
     CDS             complement(4253..5095)
                     /locus_tag="PLB65_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOQ78419.1"
                     /translation="MLNQYKVKKYIKRGIEFTFLILLVCFAFFPIFLMIINSFKNPLD
                     LWVYPPKIFTRPTLKNFSRLAKESPRFFPGLLNSIIITIGALILSLLVSFPAAFAFSR
                     YRHRLLNIPAQFLIIMRMFPPIVITIPLYPFLRSLGLVDNHLTLIVINAVFSLSLATF
                     MMKTFVDDVPIELEEAAMIEGCSKWKAFIRITLPLTAPGITAISIFVSIGIWNEYTFG
                     YIFSSTKAVTAPVVITTVSSDVMGVNWGGLFAGCVIQLLPMLIMVIAIHKYLIRGMQT
                     GAIK"
     gene            complement(5107..5760)
                     /locus_tag="PLB65_07560"
     CDS             complement(5107..5760)
                     /locus_tag="PLB65_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOQ78420.1"
                     /translation="MIGSLSFELIIGLEGAILLNKRFKFQPLWVCLILSPYAISPVVS
                     VVIWKYLLDPSYGMINYLITRFGFQPIIWFSTPLTSFIPISLVSVWKNFPFLVIILYA
                     ALTAIPEEIIEASKIDGVKGFTLFRLVTLPLILPALLVGLMFRMIFLIRTFELVWVFT
                     GGGPGRSTEILAIHLYKEAFLYLNFGKASALAWILLTITFTLSNYIMRSSNKNLIHF"
BASE COUNT         1934 a         1129 c         1027 g         1729 t
ORIGIN      
        1 tcaaggacga catcccggtc gaggtctatc cgatgaagac cctaccccga ctggtaaacg
       61 agctggccaa agagaacggg tttgagtggt ttgattagct gaaggataat aacaaaagca
      121 tcacttgcag tactcctttt accagtaaaa gaggtaccgt atttggtatg tcaaagaaaa
      181 ctagagattg atcctttctc ttttcaaagc aggaaaagct gtgaaacctt tttcaattta
      241 ctgtcaaatt gcgaacataa atatacttga tcttctcgaa tgctccctca tgaagtgatt
      301 tcctaatagt attcccataa cattcataca aatccggttt tattctttag ctcaacaatc
      361 gactcgtcat tacagatatt ccttactttc aatctcagtg gcttattctc agcccttatt
      421 gttccatccc agaattcttt tgtatctctc ccaattaacg tgatgtatcc tttttatcaa
      481 tctctatttc actgtcagaa tgttcggtca ggctatcttt ttacagcggt gaaaacatgg
      541 acataggtaa aaatgaactg cataattata atgccataag ccaaaatgtt ttgaatagta
      601 tacttggttg tgacatgata aaagatctga gaaaattagc aaacaagatt gtctccattc
      661 agatactgat cacccatacg atatgtttca tatccattgg tagcataatt cctcagttta
      721 catatcccac tgtgctatta tcccagatac gatttttatt agtaattgct actttgatct
      781 tactatatat aaattttaaa aaagacgcga gatatgtatt gattaaatta ctgtttacgt
      841 tcgtcttata cttagtgatt ggctatcctt ttggaccatt tctaataatt gacattttaa
      901 tgatgatcaa tatccttgca gtcatcattt ttaagttaga cacgccattt gatttgattc
      961 tttcaatact tgggattgct ttttcaataa tacaaaatcc cttcaagatt tttggatcca
     1021 cggtgtttgg aatccctttg gctactgttg ataacgttca tctcactgaa tacgcttttg
     1081 tgttaatatt ggtagtgttc gcatattaca tttttaagat caacatcaag gtatctgaac
     1141 acaactcaag tgaaattgaa agattatata caaatatcta ccgcttaacg aaaatgaatg
     1201 tcgttttcca ggataaagcc atatctttga aagcggaatc agcatctaag gagcgaaata
     1261 gaatcatcag ggaagttcat gacataaccg gttattattt cacgaatatt ttgatgttat
     1321 taaaaactac ccttttactt gtaaaacaaa acgataacaa atcggttcaa ttgcttgaaa
     1381 atgccattaa gcaagttcta caaggtattg atgaaattag aaggacttta aaaatcttaa
     1441 acacaatatc atctcaaaaa atcgctagca atgttaattc cttgttagaa ttgacccaag
     1501 catttcaata ccttactgac ataaaagtag agttgttgtt cggtaatatt gccaagacat
     1561 atggaacaac cgcagatctc acgatctata ggtttgtgca agaaggttta acaaatgctt
     1621 ttaggcacgg cagagctacg aaaattgaag tgcaattttg ggctacaaca gaactcttga
     1681 cgataaatat tcttgataat ggtatgggag ccttcaaatt taacaaaggc atgggaatat
     1741 cgggtatgga agaacgccta tcaaggatcg gtggaactct tgaagcagga aatatttctg
     1801 atggatttca aatatcagct ttcatacctt tgccaataca tgaggaacaa gaatgaacaa
     1861 aactataaaa gtcctcctag ctgatgatca aaaactgttc attgatagtc tgaaaaccgc
     1921 aatagaatgc ctgtctgatg atatacagat cattggcatc gcctacaatg gacttgaagt
     1981 tatcgatctt attcataagg aaattccaga cataattttg ctggatgtca gaatgccaaa
     2041 gatggatgga gttgagacag caaaatacat atcggttaat tacccaaaaa ttaaaatcgt
     2101 gatgctaacg acctttgatg atgatgatta tgtgcaagaa gcattgaagt acaatgttgt
     2161 tgggtacatt ctaaaagata ctccattaac tgaactcatt accatgatac cgattgtaaa
     2221 tgagggaact gtactgattt cacagaaagt agctaaaaaa ctgctctcag gacaccacca
     2281 taaaaatatg ggacaatctg catccataac tgatgaaaaa cttgattcga tgcctgaatg
     2341 gtatgatgaa ctgacaccca gagagctaga aatattggtg tgtgtggctc aaggtctcga
     2401 taatggagaa attgctgata gcttgtgcat tgctgaacag acagtaaaaa accatataag
     2461 cataatctat tcgaaattgg gagttcacaa acgtttgcag gcaatgcgtt tgggaagttc
     2521 gctattataa ggattcttaa tctcaaacgt cagtatcttt cggattagat atctcgccct
     2581 cccgtaaaac caggacctcc ggcagcaaca tacccgttac ttgcaccagt ttctgaagca
     2641 gaaatccaga aggaatataa ttctccattt cttaaataga atcgtatatg tattggttgc
     2701 ttcttaacat ttacaagact atttcgttct ttccattgaa tttcaacttt agttttatcc
     2761 gatgaaacag gaagacaatc ataccgacta taaccttcaa tgattctccc attaacatct
     2821 aatatctcta cttgcagttc cccatctgaa aaatttccgt taataaacaa gtgtgttcca
     2881 gagaagctca atagtcgggt cgtaagcctg ccttcttcat cttttggttt catgaaagca
     2941 aaaccatcac gacgtaaagt ggccaaccct gtactcgctc ccgcatacat tacgttcctc
     3001 attcgtccag gagaaccggt ttcatcattt tttagattgg gggattctcc agaaaaacca
     3061 gtaaaataga agtataattc gtcattaaca actaaacata ttcccccagc tgcgtgtagg
     3121 tatgctcttc tccaatcacc aacaacccta ctggatccaa tgaaaggtgt tcgatctggc
     3181 cgagtaaaat gaaatccatc tctgctataa ccgagctcta aatcaatggt ctttggaatc
     3241 cccaaatctt tgcaaatatt attctctggc cctctgaata tgccaaaaag acctatcatt
     3301 agactctcat aagctacagc attcacatca tacagagtca caacatgatt tggaaaagct
     3361 ggatcgggaa gatcatgagt atctgccagg tgccataata cttcggaaga tgaaacatcc
     3421 caatttgcac ctttcataaa atcatctgat tcggtataaa acctcgatct gataccgatt
     3481 ggaagtaatt tattattact ctcaatagct cttctgatgc tcatacacca ctttttccgg
     3541 aaaggattat agaaaaagga tgtattatct ccaactggag tagtagatac cggtgtagac
     3601 cagtgtattc cgtcctttga tgtctgaagc catgagactt ctgaatctcc atcataaaat
     3661 acatcagaat tataacggta aaattgaaac atcttaaatt tctgttcggg atttgaagca
     3721 tttccatcca accagaccag acacccgtct cgttcatagc tcttctgagt cttccaaacg
     3781 agattagttc ctggaactac atctagctgt ggacgagtcc atcgtattcc atcaacactc
     3841 gttgctatag ctgttgtgtg aaaccatccc gccatgtacc acattttgaa taactgatct
     3901 gatgaatcgt accagactcc atcattaaaa ggagcagcca tcggacaatt tcctctatcc
     3961 atttcttcat aagtctcagg aataagaata ggactggagg gatgtatttt gggtttcaca
     4021 aaaaatctga cacaatcagt ttcttcaatt aagaaatcat ctacaaataa ttgtcttcca
     4081 atatctatcg gaataatatg gggtggattt tccagataag gggcatcgaa tgcagaaaaa
     4141 gtatctacat tctgatttct tggaggccat tcaacatcca gcttaatacc attgtataac
     4201 acctcggtag ctattgtaga catattcaac ctcgcataat aataatcgaa aactatttga
     4261 tcgctccggt ttgcattcca cgaatcagat atttgtgtat tgcgattacc ataatgagca
     4321 ttgggagcag ttgaattaca caacctgcaa aaagacctcc ccaattaact cccattacat
     4381 cagagctgac agtagtaatc acaacgggtg cggtaaccgc ttttgttgaa gaaaaaatat
     4441 agccgaatgt atactcattc catattccta ttgaaacaaa tatggatatt gctgtaatcc
     4501 caggagctgt caaaggtaaa gtaattctta taaacgcttt ccatttggaa caaccttcga
     4561 tcatcgcagc ttcttcaagt tcaattggga cgtcatcaac gaatgttttc atcatgaatg
     4621 tagcgagact taacgaaaaa actgcattta tcacgattaa agtcaaatga ttatctacga
     4681 gacctagtga acgaaggaag gggtataaag gaatagttat tacgatagga ggaaacattc
     4741 gcatgataat aagaaattgc gcaggaatgt ttaacaatct gtgtcgatac cgagagaaag
     4801 caaatgctgc cggaaaagat accaacaatg aaagtatcaa cgcgccaata gtaataatta
     4861 tgctattcaa aagcccagga aagaatcttg ggctttcttt tgcgagtctg gaaaaattct
     4921 ttaatgtcgg acgagtaaaa atttttggag gatataccca taggtcaaga ggattcttga
     4981 atgaatttat aatcatgaga aatattggaa aaaaagcaaa acaaactaat agtatcaaga
     5041 aggtaaattc aatacctctc tttatatatt tttttacttt gtattggttt aacatgcaaa
     5101 acctcattaa aaatgaatca gattcttatt cgaacttcgc attatataat ttgaaagagt
     5161 aaatgttata gtgagaagga tccaagctaa tgcagacgct tttccaaaat tcaaatataa
     5221 aaaagcttcc ttatatagat gaatagcaag aatctcggtg ctacgtcccg gcccccctcc
     5281 agtgaaaacc caaaccaact cgaaagttct aatcagaaaa atcattctga acattagtcc
     5341 gaccagcagt gcaggtaaga taagcgggag tgtcactagc cgaaagagtg taaatccctt
     5401 cacaccatca atctttgaag cctcaataat ttcttctggg attgccgtta acgctgcgta
     5461 gagaataatt acaaggaacg gaaaattttt ccaaacactg acaagcgaaa ttggaataaa
     5521 tgaagtaaga ggcgtactga accaaattat cggctgaaag ccaaatctag taatcagata
     5581 gttgatcatc ccataacttg gatcaagtaa atacttccaa atcactactg agacaactgg
     5641 acttattgca tatggagata gaattaagca tacccataaa ggttgaaact taaaccgctt
     5701 gttaaggagg attgctcctt cgagtccaat aatcagttca aatgacaatg agccaatcaa
     5761 aaaatatggt tattcgctat tttgtgtggg taacccctac atgattgttg ctttaggaa
//
LOCUS       DAOSTH010000092         5792 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_12639_length_5792_cov_10.635349, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000092 DAOSTH010000000
VERSION     DAOSTH010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12639_length_5792_cov_10.635349"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PLB65_07565"
     CDS             <1..341
                     /locus_tag="PLB65_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78421.1"
                     /translation="FINTTPWGYDLYRGVDGGGWILPKTGRWSLAPTIFYPFGGDQAF
                     KAQMSVWGEKAANLKTCDEEFWELVQEADLQYLYLREGKGSLQVAALQGCAGLKRLYT
                     AGGVSIWLIE"
     gene            complement(620..1102)
                     /locus_tag="PLB65_07570"
     CDS             complement(620..1102)
                     /locus_tag="PLB65_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78422.1"
                     /translation="MKKQLLPIIIVCLSLTGCANNGKIETVVTPTANELIEFEVPEPI
                     PQPISGKGSVTGQILTDNSQELVGLIVYLGELIQLPENTYGAYLDTKSAPSSLISIEN
                     GKFYFKDVEPGIYSLIIYEVMMGGKVYQDTNGSAIPIEVNEGEITNVGKIKFFFRGNE
                     "
     gene            complement(1198..2679)
                     /locus_tag="PLB65_07575"
     CDS             complement(1198..2679)
                     /locus_tag="PLB65_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78423.1"
                     /translation="MKKFFVYAMIGLIFIIGLNPTIAYALPAGDWASGIKIQNLDPAN
                     AANLAVDLYDEEGTLEISITTTANGNPLIAPIGGSIELYMPAYVSVPSGQYSAVVSSS
                     TAVGAVVTSTNYESGMADSYASMEPSTAISIPYVYHNHNHYHTEITLLNTTNNEATVV
                     VNFKEPSSSVAFGDSGSHEKSINITIAGNGSITIDTSTAQFNDLGWFIGAASLTSTQP
                     LTAVANQYRVVGSGDTKGNLMISSRGLSSADSGTQLVVPSLYKQFIGASGTWQSGITI
                     QNPNDSTASVQVVFTADPDMPAWTGTKSLTINANDSVELYLPGVILDSEDPIPDMFKG
                     SAIITSDVSIVANVQHTNYSGANGYGVGMGYTAFSQGGKKLSLPSLYNWPSGAGVWIS
                     GIKVQNLTSTSVVFKATFVPDPDSVSQFTGTASNIALSGYGATELYFGAAFLDGGEAI
                     PSGWKGSAIIEVTTGDSDIAATVIHTNYGRHVANMYTAIVIPE"
     gene            complement(2795..3403)
                     /locus_tag="PLB65_07580"
     CDS             complement(2795..3403)
                     /locus_tag="PLB65_07580"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ78424.1"
                     /translation="MKSKNSVITGNYFNKYKSKNLLYSILVDHYRFALQSLIQDLPID
                     TCLEIGSGEGYIIDYILSTKPEIQIIGSDIGFSVVSLAKTDRQFVKWCVSDGKNIPIT
                     ENSFDIVFACEVLEHIDKPIDVLKEINRVSKKYVIISVPFEPFWRIMNIMRLKYLSSL
                     GNTPGHINHWSINGITKLVEVYFQPIDVRISFPWIFIKAQKR"
     gene            complement(3545..5449)
                     /locus_tag="PLB65_07585"
     CDS             complement(3545..5449)
                     /locus_tag="PLB65_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78425.1"
                     /translation="MDNPITIVDISFISWLRLLFGLILFLGPGKLLLSISPFRKEFDR
                     SIAFIVTFGYSVAIWAILLSISYLFKINIPTHIAIFFYLLCWLIYIIIDKPHKSYSLN
                     LNKVSFYRISLWIFIFLTFMIRIWIVRFEVAGLGSDSYHHTLFAQMIRDNGMIPMNMG
                     SNYPVITFTYHFGFHAIVAFLGWISGLPTRLLVLILGYILLAICSLAVGFSTERITGS
                     KIAGFAATICVACFFIFPSYMLMWGRYTQLTGLTLISIFLIPFWSWINNDYSKSGIIE
                     LGILAAGTGLTHYRMVAFLVIGVLVIVVFQLLGKETSNWKKGIKGGILLFFTSLSFLV
                     PWFIHLWQSFQVGYPVEASLPASAFFSIDRLGQEALNYPLNLAALILVGISILLGILH
                     KDKIIIAITLWCLIALLPSRNIQLLDTVSVLISLGIPLAILVGVAISKFCDFITSSKF
                     SPFIKNIITPVLVGALGISGIIGTLTYPTTLDSYLKASDLKAFEFINEDIPMDAKFMI
                     NIYRFNFSDILMVGSDGGYWIPLLTGRETVVPPMVFTNERVSDPIFVENLRMLGKLNG
                     DLTSPESLDMLSYQGITHVYIGERGGPINPVDLLSSPYFKLVYESNSVYIFEYNQKGA
                     FPRSLLRKIE"
BASE COUNT         1899 a         1125 c         1023 g         1745 t
ORIGIN      
        1 tttttatcaa caccacgccg tggggctatg acctataccg cggcgtagat gggggaggat
       61 ggattttgcc aaaaacgggg cgatggagcc tagcaccaac catcttttac cctttcggcg
      121 gggatcaggc tttcaaagcg cagatgagcg tttgggggga gaaagctgct aacctcaaaa
      181 cttgcgatga ggaattttgg gaactggtgc aggaggctga tctccaatac ctctaccttc
      241 gcgagggcaa aggcagcctg caggttgctg cactgcaagg ctgtgccggc ctcaagcggc
      301 tctatacagc cggcggcgta agcatttggc tgattgagta aaaaatgagt ttaggaacag
      361 agtggaggac tgggtagagt taatgagcat ctgtagaatg cactcagcat taattcaggg
      421 tatcatagaa ttgaattgag agaaaacgaa tacttaatca ctaggagttt atgatcgtct
      481 gctatttcgg catatcttgt ggaattattt ttaatattac atgttaattg taaaaaccac
      541 catttcgaat aaaagtaatc tccaagtatt aaaaatgggg taaacagtaa ttattataag
      601 attaataata ataatataat tactcgttcc ctctaaagaa aaatttaatc ttgcccacgt
      661 tagtaatttc accttcattg acttcaattg gtattgcgct tccatttgta tcctggtaaa
      721 cttttccccc catcattact tcataaatga ttaatgaata aattcctggc tcgacatcct
      781 taaaatagaa tttgccattt tctattgaaa ttagtgaaga tggtgcagat ttggtatcaa
      841 gatacgctcc atatgtattt tcgggaagtt gaattaactc gcccagataa acaattaatc
      901 ctaccaattc ttgtgaatta tctgtaagaa tttgtccagt aacagaacct tttcctgata
      961 ttggttgagg tattggctcg ggtacttcaa attctattaa ttcattggca gtgggagtaa
     1021 ctactgtctc tatcttacca ttattggcac aacctgttaa actaaggcaa acaataatta
     1081 ttggtaataa ttgttttttc atagaatgat tatatcttga atagagttag aatattaatt
     1141 aagtaacacg ccgggaaaca tactcgacgt gttacttatt ctgagttatc agttttatta
     1201 ttcgggaatc acgattgctg tatacatatt tgcaacatgg cgtccataat tagtatggat
     1261 aactgttgca gcaatatctg aatctccggt tgtaacttcg attatagccg aacccttcca
     1321 gcctgaggga atggcttcac ctccatctag gaatgcagca ccaaaatata actcggttgc
     1381 accataacct gaaagggcaa tgtttgaagc agttccagta aattggctca cagaatctgg
     1441 gtccggaacg aaagtagctt tgaatacaac agaagtagat gttaaattct gtaccttgat
     1501 cccagaaatc catacccctg cccctgaggg ccagttatat aaggatggca agcttaattt
     1561 ctttccgccc tggctaaatg cggtgtaacc cattccaacg ccataaccat ttgcacccga
     1621 gtaatttgta tgttgaacat tagccactat agagacatct gatgtaatta tcgctgaccc
     1681 cttaaacata tcaggaatag gatcttcgga atcaaggatt acacccggta agtataattc
     1741 aacactatca ttagcattaa tagttaagct ttttgttcca gtccatgctg gcatatctgg
     1801 atctgcagta aaaactacct gcacactcgc cgtcgagtcg ttaggatttt gtatcgtaat
     1861 accagattgc caagttccac tagctccgat gaattgctta tataatgatg gaactactaa
     1921 ttgagtgcca ctatctgcac tagacaaacc ccttgagcta atcattaggt tacccttcgt
     1981 atcaccagaa ccaacaaccc tatactgatt agctactgca gttaagggtt gagttgaggt
     2041 aagcgatgct gcgccaataa accaacctaa gtcattgaac tgagcagtcg aagtatctat
     2101 tgtgatggac ccattaccgg cgatagtaat gttaattgat ttttcatgag aacccgaatc
     2161 accaaaggct actgaagagc tcggttcttt gaaatttact acaacggtcg cctcattatt
     2221 tgttgtgttc agaagagtga tttcggtgtg gtagtgatta tgattatgat aaacataggg
     2281 aatagaaata gctgttgatg gttccatcga cgcataacta tcagccatac cggattcata
     2341 gtttgtgctt gtaaccacag cgccaacggc agtagaagat gaaacgacgg ctgagtattg
     2401 gccacttgga acacttacgt acgcaggcat gtacaactca attgatccac caattggtgc
     2461 aattaatggg ttgccatttg cagtcgttgt tatggaaatc tctaatgttc cctcttcatc
     2521 atataaatct accgcaagat tagccgcatt ggcgggatct aaattttgga tcttaatccc
     2581 agatgcccaa tcccctgctg gtaaggcata ggcaatcgta ggattaagac caattataaa
     2641 tattaatcca atcattgcat aaacaaaaaa ttttttcatt ttatcctccg ttcaattttt
     2701 tataagtata cttgtctcat gccaaaaaac aataatactt atagattatt tttcgactaa
     2761 gaagaatttt acaggtattt ccattcttaa tcggttatct tttttgagct ttaataaaaa
     2821 tccacggaaa actaatccgt acgtcaatag gctgaaaata aacttctaca agcttagtta
     2881 tcccatttat tgaccaatga ttaatatgac cgggagtatt gcctagtgaa cttaaatact
     2941 ttaatctcat tatattcata atacgccaaa atggttcaaa tggtacgctt ataatgacat
     3001 attttttgct cactctatta atttctttta ggacatcaat tggtttatca atatgctcta
     3061 atacttcaca tgcgaacact atatcaaatg aattttccgt gattggtata ttcttcccat
     3121 ccgatacgca ccattttaca aattgtctat cggttttagc taaagaaacc accgaaaagc
     3181 caatatcgct accaataatt tgaatttcgg gttttgttga caaaatataa tcaataatat
     3241 atccttcgcc acttcctatt tctaagcagg tatctattgg taggtcctgg attagagatt
     3301 gaagggcaaa tctataatga tcaactaaaa tactatacaa caaatttttc gatttgtatt
     3361 tattaaagta gtttccagta ataacactat ttttactttt caatttaatt ctccgaaatt
     3421 aggcaagtaa aaattagctt ttctatctga tgtcactata agtgaccggc gttgagtaag
     3481 atttaataat aataataata taatttaagt tgttcgtcat cagcatatca cttatttatc
     3541 tatcttactc tatttttcgc agcaagctgc ggggaaatgc ccccttttga ttatattcga
     3601 aaatgtatac agagttactt tcatatacca atttaaaata cggtgaactt aataaatcca
     3661 ctggattgat tggcccaccc cgttccccta tataaacatg ggttatccct tgatagctca
     3721 acatatccag gctttctggt gaggtcaagt caccatttaa ctttccaagc attctcaaat
     3781 tttcgacaaa tataggatct gagaccctct cattggtaaa aaccattgga ggaacaaccg
     3841 tttctcttcc tgtaagcaat ggaatccagt aaccaccatc acttccaacc atcagaatat
     3901 cggaaaaatt aaaacgataa atattaatca taaactttgc gtccattggt atgtcttcat
     3961 ttataaattc gaatgctttg aggtctgacg cttttaaata actatccaat gttgtagggt
     4021 aagttaatgt gccaataatt ccgctaattc ccaatgctcc cactagaact ggtgtgataa
     4081 tatttttgat aaatggagag aatttacttg aagtaataaa atcacaaaac ttacttattg
     4141 caacaccaac aagaattgct agtggaattc ctagcgatat caaaacacta actgtatcta
     4201 gaagttggat atttcttgag ggtaataagg cgattaagca ccatagggta atcgcgatga
     4261 taattttatc tttgtggaga attcctaaga ggatagaaat tccaaccaag attaaagcag
     4321 ctaaatttaa tggataattt aaagcttcct gaccaagcct gtctattgaa aaaaaagcag
     4381 atgccggaag agatgcttct acaggataac caacttgaaa cgattgccac agatgaatga
     4441 accatggcac caaaaacgat aaggaggtaa aaaataataa aataccacct ttaatacctt
     4501 tcttccaatt ggatgtttcc ttgcctaata attggaaaac aacaataaca agcacgccaa
     4561 tgactaaaaa tgccaccatc cgataatggg ttaatcctgt cccggcggca agaattccta
     4621 gttcaatgat tcctgattta ctataatcat tatttatcca ggaccaaaat ggaattaaaa
     4681 atattgaaat taatgtcaaa ccagttaatt gagtatatcg cccccacatt agcatatagg
     4741 aaggaaaaat aaagaagcag gcaacgcaga ttgtagctgc aaaaccggca attttagaac
     4801 cagttattct ttcagttgaa aacccaacag ccagactaca gatcgctaat aaaatgtaac
     4861 ctaaaattaa aaccaataat ctggtcggaa gcccactaat ccatcctaaa aaagctacaa
     4921 ttgcatgaaa accgaagtga tatgtgaagg tgattactgg atagtttgac cccatattca
     4981 tcggaatcat tccattatct ctaatcatct gcgcaaataa agtatgatgg tagctatcac
     5041 ttcccagacc cgcaacctca aaacgaacaa tccagattcg aatcatgaaa gtgaggaaaa
     5101 tgaagatcca aagactaatt cgatagaatg aaaccttatt taaatttaga gaataagact
     5161 tatgtggttt atctataatt atataaataa gccaacataa caaataaaag aaaatagcga
     5221 tatgagtagg aatattaatt ttaaataaat agcttatact taataaaatt gcccatatcg
     5281 ctacagagta tccaaaggta actataaatg ctattgagcg atcgaactct ttccggaatg
     5341 gtgatatcga tagcaacagt tttccaggac ccaaaaatag gattaaaccg aatagaagtc
     5401 ttaaccaaga aataaaagat atatctacga ttgtaatcgg gttatccata attccaatct
     5461 tttctttccc ctttacccac aaaatttgga agcctatgtt tagttgggct atcgtctaaa
     5521 acagcgaata aatatgccca cagccctgac cctggacgct ttattacaaa aagcgccaca
     5581 ctggggtgaa ttaatacagt aaagtaattc cgccaggaag gaataaaaag ccgaattcgg
     5641 ctattgcttt ctcgttcttg atctgctaat tttttgtacc tcttattctg cacacaaata
     5701 attttatctc atcatccgtt gcactagatc actgaaacca aatttgccct caaataattc
     5761 cagggcataa tcggtgggag aagagtcgag ct
//
LOCUS       DAOSTH010000093         5741 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_12761_length_5741_cov_15.639817, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000093 DAOSTH010000000
VERSION     DAOSTH010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5741
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12761_length_5741_cov_15.639817"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            326..2293
                     /locus_tag="PLB65_07590"
     CDS             326..2293
                     /locus_tag="PLB65_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78426.1"
                     /translation="MKRLSLIFAVLILFISLNAQFSNSASTPTLFAGGTGEQVIPKIA
                     ICNNGNIYLARFDNANGGYQVWLQYMDAAGNLLWQNPQGILISNFPQMTWLTEWDLDV
                     DNLGNAWLTFQDIRTAEVNNVMLYKVDASGNILLSDSGLALSSDTSTDFSNMSPLLMC
                     HSSGDVYVAWQRMSTTTEIHLQKISSTGDIYWGVNGLVFAEDAVQYTWPQLLDSGDGN
                     LLLKYYVDSGPFWAPNRQVFVTKLTPDGTPLWNASINTVGGLTAWEQIIPYVSDGNGG
                     AVLAWYDDRNNDMINEVYMARVNSAGTITTPENGALISNDTFNQQYYPKLAVDSEHGY
                     VYAWFRTTDPDQNNVGLARQLMDFSGNRLWGDNGVQIIDQGTLYPDPIAAYFYQDTAY
                     CYYSLAASPGDANNEHLKVNAIRSDQSSPWSADVFLDSSGSSKLHYDLGVSNQGWSVL
                     AYEEGNNYDIYAMRINPDGTLGSAYYPAPQSLAGYVDNQGIVYLNWQAPSLAPSQYQV
                     YMNDALILTISADLTNCSIPDLEAGNYSFYVCAVYPPDHISLPSNTINLTVLPEAVED
                     GTQNPAIPQLAVNPNPFKNNLTISYWQDKSPAQLTIYNLRGQKIYEQSFAGGQNSFVA
                     QNLHLDLPSGVYLFSLVIKGERLQKKVSVIK"
     gene            2381..4432
                     /locus_tag="PLB65_07595"
     CDS             2381..4432
                     /locus_tag="PLB65_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOQ78427.1"
                     /translation="MSFNTSSMFIESINNMESRMVLRSVMRLSAIIVYLLLFASLNAE
                     VITIGYDNYLNQGLPIEPVARFTYSQQLFLPSEIGCSGLIASVAFQYNVVSNLFYNGN
                     KNWKIWMGHTSLAELETWIPVNEMQVVFDNIIPESAFTNGLPGTGWLIINLEEPFYYN
                     GMDALIISVDENSFDSGSTTDDFFCSFCPYHYAIQYLHNTINPDPNAPPETGWTLKNY
                     RSNIQLFIEAQYYAPIQPFPADGAVNIALDTNLSWVSSCSSFSLFLGTHPDSLIQVIN
                     HTPNNYWQPDNPFLYHKTYYWQVIGYTNEIEYPSAVWIFSTITEQISPPQNLTGVYNG
                     YAAELNWNPPQTNNQHHYRLYRNASFYATTTNSSYCDDHIQAGQSYYYYVTAVSNSGE
                     ESYPSNLITVTIPSQETEQILFQGFENIPAFTEDIISWQNWDLDGNATSEWEGISYPN
                     ETNPLGWLCFEPLATNPPLHNHPPYAGNKMLASICAIPPPNNDWLISPAIHLGTNSRL
                     NFVARSAFANFGLERLCVLISNTTSEIHSFTTLNSGSYISVPVQWTNYEYDLSAWDGQ
                     RIFLAWRCLSVDAMALFLDNIEVYSNSGWVSNLDILAPQTTIKSYPNPCKGTFILESN
                     ITDAFNLEIYNLKGQCLYKERGLKSFDSKRMNSDLANGIYVLKISQGGKVITIKQTVV
                     K"
     gene            complement(4594..5565)
                     /locus_tag="PLB65_07600"
     CDS             complement(4594..5565)
                     /locus_tag="PLB65_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HOQ78428.1"
                     /translation="MKKFKYHLLSLFIGLIFAVSIWQAAEPHLADFWGKTCLSIFGTS
                     FMEVSTMDKNGVPMRYYPSAGKVYDPELIASKSSLFYKTRIEETRKNAFLFYGEWLEE
                     NLDANGNIPNNFDYLKAGIKKPWFSSASNSATMLALTQRAGYLRSPETFLKARQMLYN
                     LQPEKGSLSFYNKNGGIWFQEFPTEPYSLSGMLKTLINLSEYHKLVDDSLSQNLFQQG
                     VIALQAKLPELAKHNWLDDKYHYKNKRSEHLELTKLLEEVNSATPDSVFTPYIYNYQA
                     KNRQFVLFQLISRPQPGRILGFMFAWAVLYLIAYLFLKPKKSDIGEL"
BASE COUNT         1652 a         1111 c         1118 g         1860 t
ORIGIN      
        1 tcgtgtcgcc ctaaggggct taatcctaaa atccttgttt ctaaaaaaat cctctaaatc
       61 ctaaaatcct taaatcctcg tttcgaaaac taaatctgcg tgatctgcgt gctcacattt
      121 tttcggtgtt ctcggtggct tattaataaa tcctttaaat cctaaaatcc ttaaatcctt
      181 gtttcgaaaa tccaatccta aaatccttgt ttctaaaacc aagtctgtcg tcccgcttta
      241 atagcgtcac tttttttaaa tttgcattag caaaaaaatt gagtatcata tcttaaaatg
      301 ctgcttactc tatacgaggt gaaaaatgaa acgactatcc cttatttttg cagtccttat
      361 cttatttatc agcttaaatg ctcagttcag caattctgct tctacgccaa ctttgtttgc
      421 tggtggaaca ggagaacagg ttatccctaa aatagcaatt tgcaataatg gcaatatcta
      481 tcttgcccgt tttgacaatg ccaatggcgg ttatcaggtc tggttgcaat atatggatgc
      541 tgccggtaat cttttgtggc aaaatcccca aggaatttta atcagcaact ttccccagat
      601 gacttggcta acggaatggg atttagatgt tgataatttg ggtaatgcct ggcttacttt
      661 tcaggacatt cgtactgcgg aagttaataa tgtaatgctt tataaagtgg atgccagtgg
      721 taatatctta ctttcggata gtggccttgc tctttcctct gatacttcca cggattttag
      781 taatatgagt cctcttctta tgtgtcattc cagtggtgat gtttatgttg cctggcaaag
      841 aatgtctaca acaacggaaa ttcacttaca aaaaatatct tcaactggag atatttattg
      901 gggagtaaac ggtcttgttt ttgcggaaga tgccgttcaa tatacctggc cccaattact
      961 ggattctggg gatggtaatt tactgctgaa atattatgtg gacagcggtc ctttttgggc
     1021 tcccaatcgt caggttttcg taaccaaact tactcccgat ggaacacccc tttggaatgc
     1081 ctccatcaat acagtgggtg gcttaactgc ttgggaacag ataatacctt atgtttcaga
     1141 tggaaacgga ggagcggttc ttgcttggta tgatgatcgg aataatgata tgataaatga
     1201 ggtctatatg gcaagggtaa attctgccgg aaccattacc actcccgaaa acggagctct
     1261 tatttccaat gatactttca atcagcaata ttatcccaaa ctggctgttg attctgaaca
     1321 cggttatgtt tatgcttggt ttcgtactac cgatcctgat caaaataatg taggacttgc
     1381 cagacagctt atggattttt cgggaaaccg tctttggggc gataatggag ttcagataat
     1441 tgatcaaggc actttatatc ccgatcccat tgctgcttat ttttatcagg atacagcata
     1501 ttgttattat tctttggcag caagcccggg agatgctaat aacgagcatt tgaaagttaa
     1561 tgccattaga agtgatcaaa gcagtccctg gtctgccgat gtttttcttg attcctccgg
     1621 aagttccaaa ttgcattatg atttaggtgt ttccaatcag ggctggagcg tgcttgctta
     1681 cgaagaaggt aataactatg atatttatgc gatgcggata aatcccgatg gcactttggg
     1741 aagtgcatat tatcctgctc ctcaatccct tgcaggttat gtagataatc agggaatcgt
     1801 ctatttaaat tggcaagcgc cttcccttgc gccaagccaa tatcaggtct atatgaacga
     1861 tgccttaatt ttaactattt ctgcagattt aaccaattgc tccattcccg atttggaagc
     1921 gggaaattac tctttttatg tgtgtgccgt gtatccgcca gaccatatat cgttaccctc
     1981 caataccatt aatttaactg ttcttccgga agcggtggaa gatggaactc aaaatcctgc
     2041 cattccgcaa ctggcagtta atcccaatcc gttcaaaaac aatctcacca tcagttactg
     2101 gcaggataaa tcccctgccc aattaactat ttataatctg cgtggacaaa agatttatga
     2161 acagagcttt gcgggtggac aaaacagttt cgtagcgcag aatttacact tagatttgcc
     2221 cagtggagtt tatcttttta gcttagttat aaagggagag cgtcttcaga agaaagtttc
     2281 agtcatcaaa taagaaacta aaaacaaaga acaatataga ataatgaaaa gaggacaaac
     2341 tgaatggtga gctgattttc gaagtgttaa agcaacataa atgagcttta acacttcttc
     2401 tatgtttata gagagtatta ataatatgga aagtagaatg gttttaagat ctgtaatgag
     2461 attgagtgct attatcgtgt atcttttgct gtttgcttcg ctgaatgctg aggtaattac
     2521 catcggctat gataattatc tgaatcaagg tctcccaatt gagccggtag cgagatttac
     2581 ttattcgcag cagttatttt tgccttccga aataggctgt tctggattaa tcgcttctgt
     2641 tgcttttcaa tataatgttg tcagtaacct tttttataat gggaataaga actggaaaat
     2701 atggatgggt catacttccc ttgcagaatt agagacctgg attccggtta acgaaatgca
     2761 ggttgttttt gataatatta ttccagaaag tgcttttact aatggtttgc cggggactgg
     2821 atggctgatt ataaatttgg aagagccctt ttattacaat ggaatggatg cgttaattat
     2881 tagtgtggat gaaaacagtt tcgattccgg aagcactact gatgattttt tttgtagttt
     2941 ttgtccctat cattatgcta tccaatatct gcataatacg ataaatccag acccaaatgc
     3001 tcctccggaa acaggctgga cgctaaaaaa ctaccgtagt aacatacagc tttttattga
     3061 agctcaatat tatgctccca ttcaaccttt tcctgcagat ggagctgtaa atatagcttt
     3121 agatacaaat ctatcctggg ttagtagctg ttcaagtttt tctctttttt taggtacgca
     3181 tcctgattct ttaattcagg ttataaatca cacacctaat aattactggc aaccggataa
     3241 tccgtttctg tatcataaaa cctattactg gcaggtaatc ggttatacta atgaaataga
     3301 atacccttcg gcggtatgga ttttttcaac cataacggaa caaatatctc caccccaaaa
     3361 tttaacagga gtttataatg gttatgctgc tgagttaaac tggaatccac cccaaactaa
     3421 taatcaacat cattatagat tatatcgaaa tgcctctttt tatgcaacta ctaccaattc
     3481 aagctattgc gatgatcata ttcaagccgg acaaagctac tattactatg ttacggcagt
     3541 aagcaattca ggagaggaaa gctatccttc caatctgata actgttacta ttccttctca
     3601 ggaaacggag caaatattgt ttcagggctt tgaaaatata cctgctttta cggaagatat
     3661 tatttcctgg cagaattggg atttggatgg caacgccact tccgagtggg aaggtatttc
     3721 ttacccaaac gagacaaatc ctttaggttg gctttgtttt gagccacttg ctacaaatcc
     3781 accattgcat aatcatccgc cctatgctgg aaataaaatg ttggcttcaa tttgtgctat
     3841 tccaccgccg aataatgact ggctaatttc tccggcgata catttgggaa ctaattcccg
     3901 cctgaatttt gtggcaagat ccgcttttgc caattttggc ttggaacggc tatgtgtatt
     3961 aatttccaat accacaagcg agattcattc ctttactacc ttgaattctg gttcatacat
     4021 ctcagttccg gtgcagtgga caaattatga atacgattta tctgcttggg atggacaacg
     4081 catttttctt gcttggcgtt gcctttctgt tgatgcaatg gctctatttt tagataacat
     4141 agaagtgtat agtaattcag gatgggtttc taatctggat atccttgccc cccaaacgac
     4201 aattaaatct tatcctaatc cctgtaaagg aacctttatc ctggaaagta atattactga
     4261 cgcttttaat ttggaaattt ataacctgaa aggacaatgc ctgtataaag aaagaggatt
     4321 aaaaagcttt gatagtaaaa gaatgaatag cgatttggct aatggtatct atgtgctgaa
     4381 aatttcacaa ggcggaaaag tgattactat taagcagaca gtggttaaat gagaaaaaag
     4441 gtaatcatct caggtaacgc tgattgaatt ttaaacctct gaaatcactg ctgaataatt
     4501 atttatacag gatattccat atcctggaaa ccagataaat tctcgcttta ttcgctatct
     4561 ggcttgattt taaattataa tcctgttccc gctttatagt tcgcctatat cacttttctt
     4621 cggtttcaaa aacaggtagg caatcaaata taaaacagcc caggcaaaca taaagcccag
     4681 aattcttccg ggttggggac ggctgatcag ttgaaatagg acaaactgtc tgttttttgc
     4741 ctgatagtta taaatatagg gagtaaagac agaatcggga gtggcagaat tcacttcttc
     4801 caaaagcttg gtaagttcca gatgttcact gcgtttattt ttatagtgat atttatcatc
     4861 cagccagtta tgttttgcca gttcaggtaa ttttgcctgc agagcgatga ctccttgctg
     4921 aaagaggttt tgtgacaagc tatcatcaac caatttatga tattcagata gatttatcaa
     4981 ggttttcagc attccggaaa gcgaataagg ttcagttgga aattcttgaa accagattcc
     5041 accattttta ttgtaaaagc tcagcgaacc tttttctggc tgaaggttat acagcatttg
     5101 tctggccttt aaaaatgttt ctggactgcg taaataacct gctctttgag tcaaagcaag
     5161 cattgttgca ctattactgg cagaactgaa ccaaggtttc tttattccag ctttgaggta
     5221 atcgaaatta ttaggtatat ttccattagc gtccagattt tcttccaacc attcaccata
     5281 aaatagaaaa gcatttttac gagtttcttc tatgcgggtt ttataaaata gggaggactt
     5341 tgaggcaatt agttctggat cataaacttt tcctgcagaa ggataataac gcatcggaac
     5401 gccgttttta tccatagtgg atacttccat aaaacttgta ccaaatatac ttaaacaggt
     5461 ctttccccaa aaatcggcaa gatggggttc ggctgcttgc caaattgata cagcaaaaat
     5521 aagacctatg aataatgaga gcaagtggta tttaaatttt ttcatctttc ccttcttgcg
     5581 gcggatcggc gtcctccggc cacacttttt tttgcggcgg atcagcgtcc gccggctaca
     5641 catttttctt gcggcggatc ggcgtccgcc ggctacacat tttttcttgc ggcggatcgg
     5701 cgtccgccgg ctacactaaa tttcatttga tttatcaatc t
//
LOCUS       DAOSTH010000094         5635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_13081_length_5635_cov_17.035842, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000094 DAOSTH010000000
VERSION     DAOSTH010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13081_length_5635_cov_17.035842"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(186..2609)
                     /locus_tag="PLB65_07605"
     CDS             complement(186..2609)
                     /locus_tag="PLB65_07605"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HOQ78429.1"
                     /translation="MKVDIEHLLPLVEKPSRYIDHEINACRKDFSAFPIHFAFAFPDT
                     YELGFSHLGIKILYSIINQLDYAMADRVYLPWIDLIEKMQDEGLELFGWESRRQIKDF
                     DILGITLQSELTFTNVLELINLSGIEIHRDKRKETDPIILAGGPCATNPLPLAPFIDV
                     FFIGEAEDGIIEIAEILKTESNRTERIKQMAQLKSCYVPSLHNGTELIQSRKYINFPS
                     SEAQHTPQLLPWQLATHNRLTSEIMRGCTRGCRFCFAGYFYRPVREREPQTIIENILK
                     EIKESGWDEVGLLSLSSSDYSCIQNLLQQLLENLDTQKTHISLPSLRVDTLDNAIVQS
                     LKNLGREGLTIAPEAGSQRLRQVINKNLSEEQILAGIETALKLGWQKIKLYFMLGLPT
                     ETEEDIEAIIALIQKINNAGNRHLQINVTLSPFVPKPFTPFQWCAMQDFDTLLKNAMR
                     IKNAFARAKNIKIHYHTIENSLLEALLSRGDIKIAELIETAWKKGAHFDGWKECFNFS
                     LWQEALTECSITLDNYLGERNLQDILPWDFISLGIDKSFLISEWEKAKTGIQTKDCRE
                     ICSSCGVCKDEVQTIIAQGTFLKEGTSSTEVKKTFLQNRQYKYRVFYQKKGLLRFIAH
                     LDWMRMLFRRISLIDLQTVFTQGFSPHPKVSLCPPLAVGIEGLAEYFDLAFYQQYSPE
                     EILREFQQTPILDFTVTAVQPLNKDEKQPTSEVISVFLADDLLSQVRTKIDFFAQSKT
                     FSYTKSRPAKDKTYDLKKIILAMNLKGNELHIEKLLESPSLFDILAAILEMDKTILYQ
                     QHIYRIAFK"
     gene            complement(2606..3319)
                     /locus_tag="PLB65_07610"
     CDS             complement(2606..3319)
                     /locus_tag="PLB65_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HOQ78430.1"
                     /translation="MKIGGLQKFSLLDYPGELSAIIFTQGCNFRCPYCHNPELVDPVR
                     YSPLLDTEWVLRFLYKRHKKLSAVVITGGEPTLQEDLIPFLKLIKAMRYKIKLDTNGS
                     HPDVLQEIGNLGLVDYWAMDIKAPIKLYKVITRADLESGIIEKSMDIIRQSGIDYEFR
                     TTYFDLLLTSSDLASMQELLKPGDRFIIQQCRYENTLQEITREELLTPALSFNENPAC
                     RSLINWGTEHSVKISLRSL"
     gene            complement(3319..5454)
                     /locus_tag="PLB65_07615"
     CDS             complement(3319..5454)
                     /locus_tag="PLB65_07615"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside triphosphate reductase"
                     /protein_id="HOQ78431.1"
                     /translation="MFSSIRKRNGSIVVFDQQKIAKAIESAGFATGEFGQDIAAVLTL
                     RVLNLAQQVIQDEIPEVEKIQDIVEEVLLASPYKKTAKAYIIYRDQHSQIRNLVSKAG
                     VDLIDQYLDRLDWQVNENSNMAYSLQGLNNYIASEVSKTYWLNKIYPPEIREAHLSGD
                     LHIHDLGQLSVYCVGWDLMDLLLTGFCGAEGKVESSPAKHFRSALGQIVNFFYTLQGE
                     AAGAQAFSSFDTLLAPFIFYDGLNYKEVKQALQEFIFNINVPTRVGFQTPFTNITLDL
                     KPHQLYKDQAVIIGGKPMNKTYGDFQAEMDILNRAFLEVMIEGDAKGRVFTFPIPTYN
                     ITKDFNWDDPNLDYLWEATAKYGIPYFSNFVNSDMDPKDARSMCCRLRLDTRKLETRG
                     GGLFGANPLTGSIGVVTINLPRIGYLAKTEEEFFALLEERLQLAKKSLEIKRKVLESF
                     TQSGLYPYTKFYLKSIYERFGQYWKNHFSTIGIIGMNEAMLNFMDENIGASKAKDFAL
                     KIMDYLRNRLLEFQEETENNYNLEATPAEGTSYRLALLDKKHFPEITCANCDDKAPFY
                     TNSTQLPVNYSDDIFEVLDLQDELQTKYTGGTVLHIFGGERVEYGQSIKALVKTICEG
                     YRLPYFTFSPTFSICPTHGYLDGEQPICPLCQNRTEVFSRIVGYLRPVSQWNEGKKAE
                     FKMRTTFDLHKQPKKDSLILPETVSAKSV"
BASE COUNT         1713 a         1104 c         1038 g         1780 t
ORIGIN      
        1 ttcggtgact tcggtgttct cggtggctta ttataaaaga atcccttaaa tccttataat
       61 cctgttcatc ccagacctat tattttctct ttttgcggag gaacggcgtc ctccggctac
      121 acatttttag ctataagcaa gataccataa agaaaaaagt tttgcaggca cggatggctt
      181 cttactcact taaaggcaat tctatatata tgttgttgat agaggatagt tttgtccatt
      241 tccaaaatag cagccaaaat atcaaaaagg gagggacttt ccaacaattt ttctatatgt
      301 aattcgttac ccttaaggtt catagctaaa ataatcttct tcaaatcgta agttttatct
      361 tttgcaggtc tgcttttcgt ataggaaaat gttttgcttt gggcaaagaa atctattttt
      421 gttctaacct ggctaagtaa atcatccgct aaaaaaacac tgataacttc gctagttggt
      481 tgcttctcat ctttattcag aggttgaaca gcagttacag taaaatctaa aataggggtt
      541 tgctgaaatt cccgcaaaat ttcttccggc gaatattgtt gataaaaggc aagatcaaaa
      601 tattctgcca aaccctcaat accaacagcc aaaggagggc agagcgacac cttagggtga
      661 gggctaaaac cctgggtaaa aacagtttgc aggtcaatta gagaaatgcg gcggaaaagc
      721 attcgcatcc aatccagatg ggcaataaag cgtaaaagtc ctttcttttg ataaaaaacc
      781 cgatatttgt attgtctgtt ttgtaggaat gtttttttta cttctgtgga agatgtaccc
      841 tcctttaaaa atgttccctg ggcaattatt gtctgaactt cgtctttaca taccccacag
      901 gaagaacaaa tttctctgca atccttggtt tgaatacctg tttttgcttt ttcccattct
      961 gaaattaaaa aacttttatc tatgcccaat gatataaaat cccaaggcaa aatatcttgt
     1021 agattgcgct ctcccaagta gttatctaaa gtaatactac attcagttaa cgcctcttgc
     1081 cacaaggaaa aattaaagca ctctttccag ccatcaaaat gagctccttt tttccaggcg
     1141 gtttcaataa gttctgctat ttttatatct ccccgactaa gcaatgcttc taaaagggaa
     1201 ttttctatag tatgatagtg gattttgatg tttttagctc tggcaaaggc attttttatt
     1261 cgcatcgcat tttttagcag agtatcaaaa tcctgcatcg cgcaccattg aaaaggtgtg
     1321 aatggttttg gtacgaaagg cgaaagggtt acatttattt gcagatgcct atttccagca
     1381 ttatttattt tttgaataag agcaataatg gcttcaatat cttcttccgt ttcggtagga
     1441 agccccaaca taaaatatag cttaattttt tgccagccaa gttttagtgc ggtttcaatg
     1501 ccagcaagga tttgttcttc gcttaggttt ttgtttatca cttggcgtaa acgctgtgaa
     1561 ccagcctcgg gagcaatggt taaaccctct ctacccaaat ttttaagtga ttgaacaata
     1621 gcgttatcca aagtatccac acgcaaagaa ggcaaagaaa tatgggtttt ttgagtatcc
     1681 agattttcta aaagttgctg cagcagattt tgaatgcagg aataatcgct actggaaaga
     1741 gataacagac caacttcatc ccaccccgat tcttttattt cttttaaaat gttttctata
     1801 atagtttggg gctcgcgttc ccggaccgga cgataaaaat acccggcaaa acaaaatcta
     1861 caaccccgag tgcaacctct cataatttcg gaagttaagc gattatgagt tgccagctgc
     1921 caaggtaaaa gttggggagt atgttgtgct tcagaagaag gaaaattaat atatttacga
     1981 ctctggatga gctcggttcc attatgaaga gaaggaacat aacaagattt tagctgtgcc
     2041 atttgtttga tgcgttcagt gcggttactt tcagttttca aaatttctgc tatttcaatg
     2101 attccgtctt ccgcttcgcc aataaaaaac acatcaataa aaggagctaa gggtaaagga
     2161 tttgtggcac agggtcctcc ggcaaggata atggggtctg tttctttgcg tttgtctcta
     2221 tgtatctcaa taccgcttaa gtttatcagc tccaatacat tagtaaaagt aagttcactt
     2281 tggagagtaa tccctaaaat gtcaaaatcc ttaatttgtc ttctactttc ccaaccgaaa
     2341 agttccaaac cctcatcctg cattttttca attaaatcaa tccaaggtag ataaacccta
     2401 tccgccatag cgtaatccaa ctgatttata atggaataga gtatctttat gcctaaatgg
     2461 gaaaaaccca attcgtaggt atccggaaaa gcaaaagcaa agtgaattgg aaaagcagaa
     2521 aaatccttgc ggcaggcatt tatttcgtga tcaatatacc tggaaggttt ttccaccaaa
     2581 ggcagaaggt gttctatatc aactttcata aactgcgcaa agatattttc acgctatgtt
     2641 ctgttcccca attaattaga gagcgacaag caggattttc attaaaagaa agggcagggg
     2701 taagcaattc ttctctggta atttcttgta gagtgttttc atagcgacat tgttgaatga
     2761 taaatctatc ccccggtttt agcaattctt gcatagaagc aagatctgaa gaagttaata
     2821 gcaaatcaaa ataggtagtg cggaattcgt aatcaattcc ggattggcga ataatatcca
     2881 tacttttttc gattattcca gattctaaat ctgctctagt aataacttta tatagtttta
     2941 taggtgcttt tatatccatt gcccaataat ctaccaaacc cagattacca atttcctgca
     3001 aaacatcagg gtgagagcca ttggtatcca gttttatctt ataacgcatt gccttaatta
     3061 attttaggaa tggaataaga tcttcttgca gagttggctc ccctccggta attactactg
     3121 cggatagttt tttgtgccgt ttatacagaa agcgtaatac ccattcagta tccagtaatg
     3181 gactatatct tacaggatca accagttcgg gattgtgaca ataggggcag cgaaaattgc
     3241 atccctgcgt aaaaattatt gctgataact cacctggata atccaacaat gaaaactttt
     3301 gcaaaccacc aattttcatt atacgctctt tgctgaaacg gtttctggta gaattagact
     3361 atcctttttc ggctgtttat gaaggtcaaa agtagtgcgc attttaaatt ctgctttttt
     3421 accttcgttc cattgggaaa cgggtcgcaa ataacctaca attctggaaa agacttctgt
     3481 cctattttgg cagagaggac aaattggctg ttcgccatcc agatagccat gagttggaca
     3541 aatactgaag gttggagaaa aagtgaaata gggcaagcga taaccctcac agatagtttt
     3601 taccagtgct tttatacttt ggccatattc aactctttct ccgccaaaga tatgtaaaac
     3661 tgttccacct gtatatttgg tttggagttc atcctgcaaa tccaaaacct cgaaaatatc
     3721 atcagaatag ttaacgggca gctgagtgct atttgtataa aaaggcgctt tgtcatcaca
     3781 attggcacaa gttatttctg ggaaatgttt tttatccaaa agtgctaaac gatagcttgt
     3841 tccttcggca ggagttgctt ccaagttata attattttct gtttcttcct gaaattccag
     3901 taaacggttg cgtagataat ccataatttt cagggcaaag tccttagctt ttgaagctcc
     3961 tatattttca tccataaagt ttagcatcgc ttcgttcatt ccgataattc cgatggtgga
     4021 aaaatgattt ttccagtatt ggccaaagcg ttcataaatg ctttttaaat agaactttgt
     4081 atagggataa agtccgcttt gcgtaaagga ttctaaaact ttgcgtttaa tttccagcga
     4141 cttttttgct agttgtaaac gctcttccaa gagagcaaaa aattcctctt ccgtttttgc
     4201 tagataacct atgcggggaa gatttatggt taccacaccg atggaaccag ttagaggatt
     4261 ggcaccaaaa agtccacccc ctctggtttc cagcttacgg gtatccaaac gtaaacgaca
     4321 acacatactg cgtgcatctt taggatccat atcggaattt acaaaattgg aaaaataggg
     4381 aatgccatat ttggctgttg cctcccataa gtaatccaga ttaggatcat cccagttaaa
     4441 atcttttgtg atattatagg ttggaatggg aaaagtaaaa acgcgtccct tggcatcacc
     4501 ttcaatcatc acttccaaaa aagctctatt caaaatgtcc atttctgcct gaaaatcacc
     4561 ataggtttta ttcatcggtt tgccacctat gataactgcc tgatctttat ataattgatg
     4621 tggctttaag tctaaagtta tattagtaaa tggggtctga aaaccaactc gcgtaggcac
     4681 attgatatta aagatgaatt cctgcagcgc ttgtttcact tctttatagt taagcccatc
     4741 ataaaaaata aagggtgcca aaagtgtatc aaaactggaa aatgcttgtg ctcctgcagc
     4801 ttcaccttgc aaagtataaa agaaattaac aatctgtcct aaggcgcttc taaaatgttt
     4861 ggcaggagaa ctttccactt tgccttctgc accgcaaaaa ccggttaaaa gtaaatccat
     4921 caaatcccag cccacacaat aaactgaaag ctgacccaaa tcgtgaatat gtaaatcgcc
     4981 acttaaatgt gcttcccgaa tttcaggagg atagatctta ttcaaccagt atgtcttact
     5041 aacttcagaa gcaatataat tgttcaagcc ctgaagtgag tatgccatat tggaattttc
     5101 gtttacctgc caatctaatc tatccagata ttgatctatc aaatccactc ccgctttgga
     5161 aaccaagttg cgaatctggg aatgctgatc acgatagata atatatgcct tggcggtttt
     5221 tttgtaagga gaagcaagca aaacttcctc tacaatatct tgaatctttt ccacctcggg
     5281 aatttcatcc tgaataacct gctgggctaa attgagcacg cgtaaagtta aaaccgcggc
     5341 aatatcctgt ccaaattcac ctgtggcaaa tccagcactt tcaatggcct tggctatttt
     5401 ttgttgatca aaaacaacga tggaaccgtt tcttttgcgt atagatgaaa acatctcata
     5461 acctctattt tataaattta atatcttatt tagatataat aagttagaat gctgagaccg
     5521 tgttttggga aatcgggtct cagcaaccga agtttatgga aatatggagt tacaatttgt
     5581 caagcggaat tgtgattttt cccgcagatt tagtttcacg cagattacgc agatt
//
LOCUS       DAOSTH010000095         5539 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_13396_length_5539_cov_11.203683, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000095 DAOSTH010000000
VERSION     DAOSTH010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5539
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13396_length_5539_cov_11.203683"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..828
                     /locus_tag="PLB65_07620"
     CDS             121..828
                     /locus_tag="PLB65_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78432.1"
                     /translation="MKVNSKKLEKYLRYFFIILTSIMLGYNFYMLQWETFWSAVMTLL
                     LFILPTILEKRIAIRIPPSLQIVILLFIFASMYLGEIHGYFYRYRWWDRMLHSASAVI
                     LSYSGFILIFTLNKDNQVDIKLSPKFMALFSFCFGLMVCTLWEIFEYGVDCIFGVDMQ
                     KARNLELIYGTFDTRLGVKDTMKDLIVDTIGALVVSLIGYFHLKRKKDEDSAFWSLHR
                     QFMKDNPELFGLVETKD"
     gene            complement(976..2259)
                     /locus_tag="PLB65_07625"
     CDS             complement(976..2259)
                     /locus_tag="PLB65_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOQ78433.1"
                     /translation="MNLSKALSRLCYLLILMVIISGCGNKKSPTGGPQDIEKPTVLAT
                     SPIEFGDISSGVIEISFSKPMDKNSLANSIYIYPPVQNKKITLDKSNLKIRLNEPLRA
                     NTNYFITLTKRLKDIYGNSLVNNQTLIFRNGKLATNRIAGIIEYEEPTDKGLPLEVTV
                     LSLDSLLVISNVIRGGAYAIDNLNPQKHILRSYIDKNQNSRYDFGVEPFYEGISDGSP
                     LANMNISMTYSDTTLARINKINVNSSRELQIVLSKDITDIKDISIYSKDNPLHIAHQL
                     IEGNIINLLTAKMDSTEYQLILTQVKDNKNNIKDKLEAKFQAKPLPDTLAPKITYTNP
                     RNGASVNTLTPVLEIHFNEIIPSSNVKAKLLCSNQEIPIKHLSGTERIHRFQPTKELE
                     NYKSHILIITSETTDFSGNHLKNKYELQFLPLQRK"
     gene            complement(2246..2848)
                     /locus_tag="PLB65_07630"
     CDS             complement(2246..2848)
                     /locus_tag="PLB65_07630"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOQ78434.1"
                     /translation="MFTGIIEATEKIISIQHFGRKKLISISKPAIFSDIEIGSSIACD
                     GICLTIVKLDNRTFTVEIMNETIQKTTAKNWSINKLLNLERALTITSRLNGHFVQGHI
                     DRELRLISHLIIEGTSYLRFEFPFREKELIVPQGSIALNGVSLTIAEIRTEYFSVAII
                     GHTLEHTNLNTIKPGEMVNVEFDIIGKYIIAQKDKIYELE"
     gene            complement(2850..3449)
                     /gene="recR"
                     /locus_tag="PLB65_07635"
     CDS             complement(2850..3449)
                     /gene="recR"
                     /locus_tag="PLB65_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOQ78435.1"
                     /translation="MLFSENFEKLIQTLNRFPGIGRKTAQRLAWFMVSQDKNFALNLA
                     ETIITTVESFKTCSKCNMLSESDPCPYCTSVERLDSHLCVVENSSDIQILEGMNEYKG
                     RYFVLGHLLSPLDGYGPEQINSQQLMERILTLHPTEIILALKPSAEGEATIHYLWELL
                     KDKEINITRLSTGIPFGGDLEYSNLNTLSNAWKRRYIVQ"
     gene            complement(3450..3773)
                     /locus_tag="PLB65_07640"
     CDS             complement(3450..3773)
                     /locus_tag="PLB65_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010330208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOQ78436.1"
                     /translation="MLPGGKNMQQLMKQAQKMQAEMMKQQEELQNTVFSASSGGGMVT
                     VNMNGQYEIVSLKIDPQVVDPEDVEMLEDLVTAAIQEVLTKVSEATNNAMSQLTSGIK
                     IPGLN"
     gene            complement(4386..5462)
                     /gene="hydE"
                     /locus_tag="PLB65_07645"
     CDS             complement(4386..5462)
                     /gene="hydE"
                     /locus_tag="PLB65_07645"
                     /inference="COORDINATES: protein motif:HMM:TIGR03956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HOQ78437.1"
                     /translation="MRPDKKDLIELLSTRGEEDLEALYKRSYEIKKKYVGTSVYLRGL
                     IELSNICSKNCYYCGIRSGNKEVNRYQISLEEAIEEAKLCLDFHYGSIVIQAGEREDK
                     PWIEFITKLVKSVKELSDNQLGITLSLGEQELDVYQQWFEAGAHRYLLRIETSNRKLY
                     QDLHPADHNFEHRLNCLHLLRQCGYQVGTGVMTGLPGQTIEDLANDILFFYDENIDML
                     GMGPFIPHHNTPFKYLIDSFNPIESLNMGLKMIAVCRIALKDVNIASTTALQAIHPEG
                     RELGLKAGANVLMPNITDTKYRAGYQLYEGKPGLNETAIQFQNELEKHIYAIGETIGY
                     DQWGDSKHFFKRINKRKSEANTEN"
BASE COUNT         1679 a          916 c          965 g         1979 t
ORIGIN      
        1 cggtgtttgt atagtgaatt aacaaaaact taaaatgctg gaatagaatc tttgtcttga
       61 caattgggag catattgata gactagttca tatgtagatt tgaaccttgg agtaaaaaga
      121 atgaaagtta actcaaaaaa gctagaaaag tatctccgtt acttctttat cattttaacc
      181 tcaattatgc ttggttataa tttttatatg ttgcagtggg aaaccttttg gtctgcagta
      241 atgactttgc tattatttat attaccgacg attttggaga agaggattgc tataaggatt
      301 cctccatcat tacagatagt gatattatta ttcatttttg cttctatgta tttaggagaa
      361 attcatggtt atttttatcg ttatcgttgg tgggaccgca tgcttcatag tgcttcagct
      421 gtgattttga gttattcagg ttttatttta atttttacat tgaataaaga caatcaagta
      481 gatattaagt taagtccgaa atttatggcg ctttttagct tttgttttgg tttaatggtt
      541 tgtactttat gggagatatt tgaatatgga gtggattgca tttttggtgt tgatatgcaa
      601 aaagcgcgga atttagagtt gatttatgga acttttgata cgcgtttagg tgttaaagat
      661 acgatgaagg atttaatagt tgatactata ggtgccttag tagtgtctct gattggttat
      721 tttcatttga aaagaaaaaa ggatgaggat tcggcttttt ggtcacttca taggcagttt
      781 atgaaagata atcccgagct tttcggatta gtggaaacaa aggattgaac agctattgag
      841 tatgatgatt tagtaatcaa caactctctt tttcccacat agtataaaac ttttatgaac
      901 ttactaaata ttaagttaga gaatcctata aatcagtata attctattta tatcagagtt
      961 actttcctgg tatatctatt ttctttgaag gggaagaaac tgtaattcgt atttgttttt
     1021 tagatggtta ccactgaagt ccgttgtttc cgaagtaata ataagtatat gtgatttgta
     1081 attttccagt tctttggtgg gctgaaaacg atggatccgt tctgttcccg acaagtgttt
     1141 aataggtatt tcctgatttg agcagagcaa tttggcttta acatttgagg aagggataat
     1201 ttcattaaag tgaatttcca gaacaggtgt aagtgtattt actgatgcac cgttacgagg
     1261 attagtataa gtgattttgg gagccaaggt atcaggcaat ggtttagctt gaaatttggc
     1321 ttccaacttg tccttaatat tgtttttatt atctttaacc tgagtaagaa tcagctgata
     1381 ttcagtgcta tccattttag ctgttagaag gttaattata tttccttcaa tcagttgatg
     1441 tgctatatgt aaaggattat ctttggaata tatgctaatg tctttaatat cagtgatatc
     1501 cttgcttaat acgatttgca attcacggct actattaaca ttaatcttgt ttattcttgc
     1561 caaggttgta tcactgtaag tcattgatat attcatattt gctaaaggag agccatcaga
     1621 tatcccttca taaaatggct caacaccgaa atcgtaacga ctattttgat ttttatcgat
     1681 ataggatcgc aagatatgtt tctgtggatt caaattatca atagcgtaag ctccacctcg
     1741 aataacatta cttatgacta aaagagaatc aagagaaaga acagtcactt ccaaaggcaa
     1801 acctttgtcg gttggttctt catattcaat aatacccgcg atacgattag tggctaattt
     1861 cccattgcga aagataaggg tttgattatt cactaaagaa ttaccatata tatctttaag
     1921 acgcttagtt aaagtaatga agtaatttgt atttgccctt aatggctcgt ttaaacggat
     1981 tttaagattt gatttatcca aagtgatttt tttattttgt acagggggat agatatagat
     2041 agaattggcc aatgagtttt tatccattgg tttagaaaag gatatttcaa taactcctga
     2101 agaaatatca ccaaattcaa taggagaagt agcaagaact gttggctttt caatatcctg
     2161 gggtcctccc gttgggctct ttttatttcc acatccactt ataataacca ttaatataag
     2221 caaatagcat agacgagata atgctttact caagttcata tatcttatcc ttttgtgcta
     2281 taatatattt accgataata tcaaattcta catttaccat ttcgccgggt tttatagtat
     2341 ttagatttgt atgttcaaga gtgtgaccta ttatagctac actaaaatac tcagtgcgaa
     2401 tttcagctat agttaaacta actccattaa gagcaattga gccttgagga acgatcagtt
     2461 ctttttccct aaaaggaaat tcaaaacgca gataagaagt tccttcaatt attaaatgtg
     2521 agattagtcg taattctcta tcgatgtgac cttgaacaaa gtgtccattc aagcgagaag
     2581 taatcgttaa agctctttcc agattcagta atttatttat actccaattt ttagcagtgg
     2641 ttttttggat tgtttcgttc ataatttcca cggtaaatgt tctattatcc aatttgacga
     2701 tcgttaagca aattccatca caagcaatgc tagaaccaat ttctatatca ctaaatattg
     2761 caggtttgga aatagatatc agttttttcc tgccaaaatg ttgaatgctg ataatttttt
     2821 ctgtagcttc gatgattccg gtaaacatac tactgtacta tgtatctacg tttccaggca
     2881 ttagataaag tattcaagtt actatattcc aagtctcctc caaaaggaat acctgtagaa
     2941 agtcgggtaa tattgatttc tttatcttta agcaattccc aaagataatg gatagttgct
     3001 tcaccttcag ctgaaggttt taaagccaag attatttcag ttggatgaag ggttaggatg
     3061 cgttccataa gttgctgtga atttatttgt tctggtccat atccatccag aggtgaaaga
     3121 agatgaccca aaacaaaata tcttccttta tattcgttca ttccttccaa aatttgaata
     3181 tctgagctat tttctactac gcataaatgg ctatccaatc tttcaacaga agtacaataa
     3241 gggcagggat cgctttccga aagcatattg cacttactgc aagttttaaa gctttctact
     3301 gtagttataa tggtctcagc caagtttaag gcaaaatttt tatcttgcga aaccataaac
     3361 caggcaagac gctgagctgt ttttcgtccg attccgggaa aacggtttag agtttgaatt
     3421 aatttttcaa aattttcgga aaagagcatc taatttaagc ctggaatttt aataccactg
     3481 gtgagttggc tcatagcatt attggttgcc tcactcactt tagtgagaac ttcctgaatt
     3541 gcagcagtaa caaggtcttc cagcatttct acatcttcag gatctacaac ctgaggatct
     3601 atttttaagg aaacaatttc atactggcca ttcatattca ctgttaccat tcctccacct
     3661 gaagaagcag aaaatacagt attttgcagt tcttcctgct gtttcatcat ttcagcctgc
     3721 atcttttggg cttgtttcat taattgttgc atattttttc caccgggaag catataaatc
     3781 tccttatctt atggttttgt tctcattctt tttattatgt ttattcgtca agaaaaacat
     3841 ttcctgccgg aactagttat tatagattac gcagctggca cagaaaaaaa gggaataagg
     3901 acaaagatag gaaagagatt tgtactgatt aatatagtcg acatagataa aattgtaatc
     3961 ttcactaaag aaagaaggtt tacatcctgc ttatatacta gccgtggagt tgccgtgggg
     4021 caattgggta aactccctaa tagttctacc tcagctctat catagctaac taagctataa
     4081 taatccagaa caatgatctc gttcccaaac attacccaaa aagagtaatc aattcaatgg
     4141 ctatgataac ttacttaata agcttataaa gttaggatgg ttgaaaatta ttaaactatc
     4201 ttgttttttt taaattctgc acgaatagga agttattatc catattttac caatagcatt
     4261 attatatttg aacagatttt agcagatcga gataagcaaa agaatatgtg gattaaatgg
     4321 attgtaacgg atttatacag gtttgaattt ttctaaacaa tctctttcca agctaattac
     4381 taagactaat tttcagtatt ggcttcactt tttctcttat ttatccgctt aaagaaatgc
     4441 tttgaatcgc cccattggtc gtaaccaatt gtctcaccga tggcatatat gtgtttttcc
     4501 agttcatttt gaaattggat agcagtttca ttaagtcccg gttttccttc atatagttga
     4561 taacccgctc tatatttggt atccgtgata tttggcatta aaacatttgc tcctgcttta
     4621 agacctaatt ctcttccttc tgggtggatg gcttggagtg cagttgttga agctatattt
     4681 acatctttta atgctatacg gcaaacagcg atcattttca atcccatatt caatgattca
     4741 atagggttaa aactatctat caaatatttg aaaggagtgt tatgatgtgg aataaaaggt
     4801 cccattccca gcatatcgat gttctcatca tagaagaaaa gaatatcgtt agctaaatct
     4861 tcaatagttt gccccggcag acctgtcatt actccagttc caacttgata accacactgt
     4921 cttaagagat gcaaacaatt caggcggtgt tcaaaattgt gatcagcagg atgcaaatct
     4981 tgatatagtt ttcggtttga agtttctatg cgtaataagt aacgatgagc tccggcttca
     5041 aaccattgct gataaacatc taattcctgc tctcccagcg aaagggttat accaagttga
     5101 ttatccgata gttcctttac gctttttact agtttggtga taaattctat ccagggttta
     5161 tcttctcgtt ctcctgcctg gataacaatt gaaccataat ggaaatctaa acatagtttg
     5221 gcttcttcaa ttgcttcttc gagactgatc tgataacgat taacttcttt atttccactg
     5281 cgaattccac aataataaca attctttgaa cagatattac tgagctcaat tagtcctcgt
     5341 aaataaacac tggttccaac atactttttt tttatctcgt aagatctttt atataaagct
     5401 tcaagatcct cttctcctct tgtagagaga agctctatta aatccttttt atctggtcgc
     5461 atattttttc ctttttacaa tatatagtaa tccatctttg tcttttggca agtaaaggat
     5521 actgattcca ccgaaaaca
//
LOCUS       DAOSTH010000096         5536 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_13404_length_5536_cov_18.510491, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000096 DAOSTH010000000
VERSION     DAOSTH010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13404_length_5536_cov_18.510491"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(298..2373)
                     /locus_tag="PLB65_07650"
     CDS             complement(298..2373)
                     /locus_tag="PLB65_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78438.1"
                     /translation="MKKFFLLALILTAGMLNATVITAWTLENTLSPATGSGTVSLIGG
                     VTDDGFNTGYNGGQGWSTTSYPTQGTNNMTAGIMIEVSTQNFQNITLSWALRHSNKSA
                     NRAVLFYTLDRTAPEPVWVQAGIYDATAGDSWFPNSFDASSIAGINNNPNLAFKLVSA
                     FANPENTLYLPSDPTKTYDGGKWRFDDIILEGTSSAPYVQITSNLQPFYALPGSVSAV
                     QTYQIIATNLTGNLVINAPQYFYLRLFDTDPFVSSLELIPRNGGFDKQIQVSFQPTES
                     GNFNGNIEHSGGGIEPQYIEVSGSTIFPEPTNYPVSFSASGITYYQTYLNWTDSTGAT
                     LPSGYLIKGSEIGLSEIADPIDGTPENDSEFIYNVSYGVQTLLLFGLKENQEYFFKIF
                     PYTNSGDNIDYKADANAPALQFTTPTGPVGSILVPGDLAFVEYATDSPDRFSFVLLKD
                     IQENTKIYFTDKAWTGTAFLANEGLYEWRGIGRSYAKGEVIHLVEGQTFTNEGIHSPG
                     VEGLSNDGDQVIAFQGTIDEPSFLAAISSTGWLTEGTVNNNSSYLPSALTLGENALGF
                     ANEVDDGVYNGPGTGSVSEIRIALNNPANWNRANNLNNITFPVWNFVLDALIMPEPLI
                     TKINDTTVRISWQEVSGASHYKVYKSALPDAIFPQEWVLIADNIMGLYWETTDITYPK
                     QFYRIVAVN"
     gene            complement(2383..5490)
                     /locus_tag="PLB65_07655"
     CDS             complement(2383..5490)
                     /locus_tag="PLB65_07655"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HOQ78439.1"
                     /translation="MKKAYFILCLLVCLAFNLVAQTDFYDINTVNTIQLFFTQSNWDQ
                     ILDQLYAAGEGRLVGTAIINGVTYDSVGVRYKGNSSYNANRNKNPFNIKLDHIIEDQL
                     IDGKYGTLKLANGFSDPSFIRETLSYEIARKYMPACKANYANVWVNNVLIGVYTSVED
                     VDSNFMREHFYTSGKPRFKCDTNTMSPVTVWGYLGADSTAYQTYYALESDYGWSTLIN
                     FTYTLQNNYTNIAQVMNVDQNLWMCAFDNLLVNLDSPINVFHNFYLFADADNRINPLL
                     WDLNMSFGGFQGGSVTSMQNLDPLRNVNSNTFLLLKNVLVNPRYKKMYLAHIRTMIEE
                     NFSNGWYATRAAELQSICGPSVQNDPNFFYTYANFQANLNNQVGGGSGGPGGGQTVPG
                     ITQLMNARATYLLNNTNFAGTVPTLTTQSYYPANPEPGSNLQFTATFTNATYAQLGIR
                     QNIAHQFNYYQMYDDGAHSDGEANDGVYGISVPITYGDINYFFWAENSSQGMFFPARA
                     EHEYYTIPVANFTGELFINEIMAKNASFADPNGEFDDWVEIYNPNNYAVDLGGMYMTD
                     SHYSNGISAWTQIPTTYPDITTIPPHGYKIVWFDEDLDQGPLHINDKLGGGADAVYLI
                     DSDGVTLIDSYIWTEATNLNVDDRSIGRLPDGSDNWVLFGAGQTNPCTPGASNQGTVN
                     ALPVIENIAYSPLTTDENSIITVSASVTDSDGTINSVQLLYGISDWTLNAIPMNLTGN
                     SYTAQIGPFAWGSIIKYRIQATDNASGITQSPVYAIIIGYSAPTLYINELMPSNATTI
                     MDENSEYEDWIEIYNPNDFAIDLAGYFFTDDHYPDTGSSLTQIPTGYNDTIIPANSYK
                     IIWFDEDLDQGPLHINTKLSATADAVYFLAPDMLTVIDHISWTADLALASDISYGRYP
                     DGSENWITFGVGFDHPVTPGTSNYPVSNTDEIVTTAVMSLEVWPNPVKDVLNINLKGA
                     TDKYRVKVYNLKGQLVADYYTTKDGKNQWDLRDKNGNKIGSGIYLIRTQFADKQYSKK
                     ICIIQ"
BASE COUNT         1720 a         1115 c         1083 g         1618 t
ORIGIN      
        1 actaattttt ccttcgccta acttattgaa agctaaattc tctccggata gttctcttga
       61 taaacgatat agctattttt gttgactatt actataaaac accgaacgag cattttgaaa
      121 aataagcaat tatattgaag gaaaatgggt tggcacattc tgatttacta aagagttaaa
      181 aaagccctta acttaaagta ttaaaggtag ttgccaaatt agtaatccgg taattcataa
      241 tataaaattg ccggttggaa atatattcca gccggcatag ttgtttaaca tagctaatca
      301 gttaactgct acaatgcgat agaactgttt gggataggtg atatcggttg tttcccaata
      361 aagtcccatg atattatccg caattaaaac ccattcctgc ggaaaaatgg catccggtaa
      421 agctgattta tagaccttat aatgcgatgc accggaaacc tcctgccagg aaatcctaac
      481 agttgtgtca tttattttgg taattagcgg ttccggcata attagggcat ccagaacaaa
      541 attccagaca gggaaagtta tattgtttaa gttattggct ctattccagt tagcaggatt
      601 attgagagct attctaattt ctgaaactga accggtacca ggaccgttat aaacgccatc
      661 gtctacttcg ttagcaaagc ccaaagcgtt ttctcccaag gtcaaagccg atggaaggta
      721 ggaactattg ttatttaccg ttccttctgt tagccatccg gttgaagaaa tggcagcaag
      781 aaaagagggc tcatctattg ttccctgaaa ggcaataacc tgatcaccat cattggaaag
      841 tccttctacg ccgggactat ggataccttc attagtaaag gtctgccctt caaccaaatg
      901 tataacttct cctttagcat aagaacgccc aattcctctc cattcataaa gcccttcatt
      961 tgccagaaaa gcagttcctg tccacgcttt atcggtaaag taaatcttgg tattttcctg
     1021 aatatctttc aacagaacga aactaaatcg gtcaggtgaa tcggttgcat attctacaaa
     1081 cgccaaatcc ccgggaacaa gaattgaacc aacgggtcct gtaggggttg taaattgaag
     1141 tgcaggagcg tttgcatccg ctttgtaatc tatgttgtct ccgctattgg tatagggaaa
     1201 aatcttaaaa aaatattcct gattttcctt caagccgaat aaaagtaggg tctgaacacc
     1261 atagctgaca ttgtatataa actcactatc gttttcagga gttccatcta tggggtctgc
     1321 aatttccgat aagccaattt cactgccttt aatcagatag ccactcggta aagttgctcc
     1381 ggtagaatca gtccaattta gatatgtttg ataataggta atcccgctgg cagaaaagga
     1441 aacaggataa ttggtcggtt cgggaaaaat ggtggaaccg cttacttcaa tgtattgtgg
     1501 ctcaattccg cctccgctat gctcaatatt gccgttaaaa ttgccacttt cggttggctg
     1561 aaagctcact tgaatctgtt tatcaaagcc accattacga ggaataagct ctaaagaaga
     1621 aacaaaagga tcggtatcaa acaaacgcag ataaaaatat tgaggtgcat ttataaccaa
     1681 atttccggtt aaattggtag caattatctg ataggtctga actgcggaaa cgcttcctgg
     1741 caaagcataa aaaggttgca aattagaggt tatttggaca taaggagcag atgaagtccc
     1801 ttccaaaata atatcatcaa aacgccattt tcctccatca taggtcttag taggatcgga
     1861 aggtagataa agggtatttt ccgggttggc aaaagcggaa acaagcttaa aagccagatt
     1921 aggattatta tttattcccg caattgagct tgcatcaaaa gaattgggaa accagctgtc
     1981 tccagcggta gcatcgtaaa ttcctgcctg aacccagacc ggttcaggag cagttctatc
     2041 cagagtataa aaaaggacag cccgattggc ggatttattg ctgtgccgaa gagcccaact
     2101 aagggttata ttctgaaaat tttgagtgga gacctcaatc attattcccg ccgtcatatt
     2161 attggttccc tgagtaggat agcttgttgt gctccaacct tgccctccat tataacctgt
     2221 attgaaaccg tcatccgtaa ctcctccgat taaagaaacg gtgccactgc cggttgcagg
     2281 actaagggta ttttccaaag tccaggcagt aataaccgta gcatttaaca tccctgccgt
     2341 taaaatgagt gctaataaaa agaacttttt cacttttgct ccttattgga tgatgcatat
     2401 ctttttgctg tattgtttat cggcaaattg tgttctgatg aggtaaattc cactgcctat
     2461 tttattgcca tttttgtcgc gtaaatccca ttggttcttt ccgtctttcg ttgtataata
     2521 atcggcaact aattgacctt taagattata cacttttact ctatatttat cggtagcgcc
     2581 tttcagattg atgtttagaa catctttaac aggattaggc caaacctcca aactcataac
     2641 cgcggtagta acaatttcat ccgtgttgga aacaggataa ttgcttgttc caggtgttac
     2701 cggatgatca aagccaactc caaaagtaat ccagttttca cttccgtcag gatacctacc
     2761 ataagaaata tcactggcta aagccaaatc tgcagtccag gaaatatgat caattaccgt
     2821 taacatatcg ggtgccagaa aatacactgc atccgctgta gcgcttaatt ttgtattgat
     2881 atgcaaagga ccctggtcaa ggtcttcatc aaaccagata attttatagc tattggcagg
     2941 aattatggta tcgttatagc ccgttggaat ttgggttagg gaactgcctg tatcgggata
     3001 atgatcatcc gtaaaaaaat aacccgccaa atcaatagca aaatcattgg ggttataaat
     3061 ctctatccag tcctcatatt cgctgttttc atccataatg gtagtggcat tggaaggcat
     3121 caattcatta atataaaggg taggtgcaga atatccaatt attatagcat aaaccggtga
     3181 ctgcgtaatt cccgaagcat tatctgttgc ctgaatgcga tatttaataa tggagcccca
     3241 agcaaaagga ccaatttgcg ctgtgtaact atttcctgtt aaattcatag gaatggcatt
     3301 caaagtccaa tcggaaatgc catacaataa ttgcacgcta tttatagttc catcactgtc
     3361 cgttacgctg gcagaaacgg ttataatgct gttttcatct gtagtaagag gactgtaagc
     3421 aatattttca attacaggaa gtgcatttac tgttccctga ttggaagcgc ccggagtgca
     3481 gggatttgtt tgtcctgctc caaataaaac ccaattatca ctgccgtcag gtaatctgcc
     3541 aatggagcgg tcatccacat taagattggt tgcctctgtc cagatatagc tatcaataag
     3601 ggtaactcca tcgctatcta ttaaatagac ggcatctgct cctccgccga gtttatcatt
     3661 gatatgcaaa ggaccctgat caaggtcttc atcaaaccac acgattttat aaccgtgagg
     3721 aggaatagtt gtgatatcag gataagtagt cggaatttga gtccaggcac taattccgtt
     3781 gctgtaatgg ctatcggtca tatacattcc gcctaaatcc acggcataat tattgggatt
     3841 atagatttct acccaatcat caaattctcc gttaggatcg gcaaaagaag catttttagc
     3901 cataatttca ttgatgaaga gctctcctgt aaaattagcc acaggaattg tgtaatattc
     3961 gtgttccgct ctggcaggaa aaaacatccc ttgagaggaa ttttccgccc agaaaaagta
     4021 attgatatcg ccataagtaa taggaacact tattccataa acaccatcat tggcttcacc
     4081 atcggaatga gctccatcat catacatttg gtaatagtta aattggtggg caatattttg
     4141 tctgataccc agctgagcat aagtagcatt ggtaaaagtt gcagtaaatt gaagatttga
     4201 acccggttca ggattggcag gataataact ttgagttgtt aaggtgggaa cagttccggc
     4261 aaaattggta ttattcaata agtaagtagc acgcgcattc atcaattgtg taattccggg
     4321 aacagtttgg cctccaccgg gacctccgga accacctcca acttgattat tcagatttgc
     4381 ctgaaaattg gcataagtat aaaagaaatt aggatcattt tgcacggacg gaccacaaat
     4441 actttgcagt tcagcagccc ttgtggcata ccagccattg gaaaaattct cttctatcat
     4501 agtgcgtata tgggcaagat acatcttttt gtaacgagga ttaaccaaca cattcttgag
     4561 cagaagaaaa gtgttactgt tcacattacg caagggatca agattttgca tactagtaac
     4621 ggaaccacct tgaaatccgc caaaggacat attcaaatcc cataaaagcg ggttaatcct
     4681 gttatcggca tcggcaaaaa gataaaagtt atgaaagaca tttattggag aatccagatt
     4741 taccaagaga ttatcaaaag cgcacatcca taaattctgg tctacattca tcacctgggc
     4801 aatattggta tagttgtttt gcaaggtata ggtaaagtta attaaagtgc tccaaccata
     4861 atctgattcc agggcataat aagtttgata ggcagtagaa tcagctccca gataacccca
     4921 aacggtaaca ggactcatag tattggtatc acatttaaag cgtggcttac ccgaagtgta
     4981 aaaatgctct ctcataaaat tgctgtccac atcttccacg gaagtataaa cacctataag
     5041 gacattattc acccacacat tagcgtaatt agctttgcag gcaggcatat atttacgggc
     5101 aatttcgtaa gaaagggttt cccgaataaa agaaggatcg gaaaaaccgt tggcaagctt
     5161 aagtgtgcca tattttccgt ctatgagctg gtcttcaata atatgatcca gtttaatatt
     5221 aaaaggattc ttgtttctat tggcattgta ggaactattg cctttataac ggactcctac
     5281 gctatcataa gttactccgt taataattgc tgtgcctact aatctaccct cacctgccgc
     5341 ataaagctgg tcaagaattt gatcccaatt ggattgagtg aaaaataact gaattgtatt
     5401 cacggtgtta atatcgtaaa aatctgtctg agccaccaga ttaaaagcaa ggcaaaccaa
     5461 caggcagaga ataaaatatg cttttttcat attcacctct ttgcaggcaa aaaaattaca
     5521 gggactgcat tataca
//
LOCUS       DAOSTH010000097         5484 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_13568_length_5484_cov_15.501381, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000097 DAOSTH010000000
VERSION     DAOSTH010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5484
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13568_length_5484_cov_15.501381"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            559..693
                     /gene="rpmH"
                     /locus_tag="PLB65_07660"
     CDS             559..693
                     /gene="rpmH"
                     /locus_tag="PLB65_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOQ78440.1"
                     /translation="MKRTYQPSNRSRKNTHGFRSRMATKNGRKVLARRRAKGRKTLTV
                     "
     gene            698..1030
                     /gene="rnpA"
                     /locus_tag="PLB65_07665"
     CDS             698..1030
                     /gene="rnpA"
                     /locus_tag="PLB65_07665"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOQ78441.1"
                     /translation="MVRWITSHSEYVDFYNPDFQFRTAYFYVPVLYSKGETAVGITIG
                     RKIGNAVKRNLLKRRIKSWLYSNQEKLPIGFRFNLVARIGAADLQWLELSQELLKVIT
                     QINTKAVY"
     gene            964..1257
                     /gene="yidD"
                     /locus_tag="PLB65_07670"
     CDS             964..1257
                     /gene="yidD"
                     /locus_tag="PLB65_07670"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOQ78442.1"
                     /translation="MAGIIARAIKSNNSDKYKSGLLRQILRLPNLLFLFLIRFYQLAF
                     SPFLPPSCRFEPSCSVYGYQAFKKYNFFKAFYLTSMRILKCNPFHKGGYDPLP"
     gene            1268..2899
                     /gene="yidC"
                     /locus_tag="PLB65_07675"
     CDS             1268..2899
                     /gene="yidC"
                     /locus_tag="PLB65_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOQ78443.1"
                     /translation="MEKRTLIALLLVFILFLVFNEFVWKPQQIRHQQQLQEQQAVTPE
                     PVKEQVKPDSSQLIPLADTLLAKGEHPEIIKLSNQYMTVTFNSRGGIIQQVELHNFVM
                     HDSTKVKLIPENASIASVKIIHPASETALQDVVFQYKISPDSKGVTFFLGEESQPKLI
                     KSFALDDQYGIALEVEVANYLVTNGIELDFGSGIADTEKTTKSKLQDYRFMFYADNEI
                     FKTTLAKMKKKQPSGTFNSFNWMALRGKYFTLVLKENEPALMRSYTSTLNPETGNPAF
                     VIDSRQSNPKRSWKQSFIIYAGPADYQILQKYGKQMDSIPERGASWLRWLSNIFAGLL
                     KFLHRYIKNYGVVILIFALIIKLLLHPLTQHQMVHSMRQQKMQPYIEEIRKKYANDVV
                     KQREELTKLYKENKTSMTAGCLPLLIQMPILIPLYSVLRYSLDMRNASFCLWLKDLSE
                     PDPYMILPIIMGGFMLLQSLMMRPRNVDTSQMTEQQKMQQSQMKIMTWGMPIFMIIIF
                     RNMPAGLVLYWTTFNVLSIIHQYFLTKHLKKKENQ"
     gene            2896..3684
                     /locus_tag="PLB65_07680"
     CDS             2896..3684
                     /locus_tag="PLB65_07680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HOQ78444.1"
                     /translation="MTTIDKSGESLEKIIKDFRNEYKIHDWELQYEILKKPSKGIFGL
                     FAHKLALVRFQLPSSLDRAKLFIQNLLEKMRIKYTSLESRMEGKTIYLDINGSPDSGF
                     LIGKNGSMLDNLQYLLNRVFENDRQVERIYLDVDGYRERQADNFLKPYLSQIVNVKLS
                     DKPVTLEPMNAAERRIIHRFVEKENGLRTLTIGEGERKRIVIFSAKQSEKEVLSKSKK
                     YSQHRSKSTDPQTKNKSGKPKSTPGKGNPKPHIKQPQMSNPKHQ"
     gene            3681..4511
                     /locus_tag="PLB65_07685"
     CDS             3681..4511
                     /locus_tag="PLB65_07685"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOQ78445.1"
                     /translation="MKQIYPVPAGFYWSDSGAFMGVLPYALWSKKSEIDERFRRKLNL
                     NLLLIQSGNRNILIDTGLGNRLSAKQREIYQPSEFLLPVSLAELGLQDKDITDVIMTH
                     LHFDHAGGIVTDFGTYEALTFPKAHYWIQKAEWEMAKNPDELNQAAYNYEQQLSLLEQ
                     KGNITLLEGEAEIEPGITLTLVGGHTVGSQIITINFGTEYFIYAGDIIPTMFHTSLAI
                     TSAYDVSRIQTVAAKKLIYSRLQEHNGILLLNHDLSHWQVPFSELKIGKKEKKDKMDS
                     "
     gene            4651..5157
                     /locus_tag="PLB65_07690"
     CDS             4651..5157
                     /locus_tag="PLB65_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78446.1"
                     /translation="MFFTNRIIKIVSFCLVGLLLSSCVKYRGIEVPVNPPPSFEKNYI
                     LLIWTQKGKSKTNPKKSTLQANELFMKNITIAGDTLTGFVYNKPNKKHIKNQISVYID
                     DSIAQPDSIPSPFSLPVSAIQKIETYEVNVGTTILTSILGIATTTLTIAIICVGYLII
                     YQIVTQYD"
BASE COUNT         1794 a         1041 c         1029 g         1620 t
ORIGIN      
        1 agaatgaaat gaggtggcag atcacataga aagccgattt taactcaaag gccgacacag
       61 tgatagcgac ggtggcgtaa gcccctcgaa aatgataata ggcataggat gaataggatt
      121 caaaggatta aaatttagaa acaaggattt taggatttaa ggattagttc acgcagatta
      181 cgcagattac gcagatataa ttttagaaac gaggatttaa ggattatagg attaagagga
      241 tttcgaattt aaagttccgc taggaacgat acatactaac gacgggtttt aacccggcga
      301 caagaagaac aaattaaaaa agtcccagcg ggacggcaga tatggttttc gaaacaagga
      361 tttaaggatt tggaggatat atcttccaga aaaaggtgtc ctccatctat ataagttcgg
      421 ggtcagcaaa tgcctaaata actttcatcc ctttctataa atctggagtt aattttaaaa
      481 attcttgaca gagcaggcac aggaaatttt atgacataat aactctaaat gcatacatct
      541 ttaaggagat acataaaaat gaaacgaact taccaaccct caaacagatc gcgaaaaaac
      601 actcacggtt ttcgcagtag aatggctacc aaaaatggtc gtaaagtatt agctcgcaga
      661 agagcaaaag ggagaaaaac tttaacagta tgagcttatg gttaggtgga ttacttccca
      721 ttctgaatat gtggattttt acaatcctga ttttcagttt cggacggcat atttttatgt
      781 gccagtatta tatagtaagg gtgaaacagc tgtgggcatt actattggta gaaaaatcgg
      841 caacgccgtt aaacgcaatt tacttaaacg cagaatcaaa tcctggctgt attccaatca
      901 ggaaaaatta cccattggtt tccgatttaa tttggtagcc agaattggag cagctgattt
      961 acaatggctg gaattatcgc aagagctatt aaaagtaata actcagataa atacaaaagc
     1021 ggtttactaa ggcagatatt gcgattaccc aatctgcttt ttttgtttct cattcgtttt
     1081 taccagctgg cattttctcc ttttttacct cccagttgcc gatttgagcc cagttgtagt
     1141 gtttacggat atcaagcgtt taagaaatat aacttcttca aagcgtttta tttaacctct
     1201 atgcgtatcc tgaaatgtaa tcctttccac aagggtggat acgatccact tccctaaagg
     1261 acaagaaatg gaaaaaagaa ctttaattgc cttactcctt gtgttcattc tctttttagt
     1321 tttcaatgaa tttgtttgga aacctcaaca gattaggcat caacagcagt tgcaagagca
     1381 acaagctgtt acccccgaac cagttaaaga acaggtaaaa cctgattcat cacagcttat
     1441 acctttggcc gatacgctgt tagcaaaagg tgaacatccc gaaataataa aactttccaa
     1501 tcagtatatg acggttactt tcaacagtcg gggtggcatt attcagcaag ttgagttgca
     1561 taattttgtt atgcacgata gcaccaaggt aaagttaatt cccgaaaatg cttccatagc
     1621 cagtgtaaag ataattcatc ctgcttcgga gacagcttta caggatgttg ttttccagta
     1681 taagatttct cctgattcta agggagtaac ctttttcttg ggcgaggaaa gccaacccaa
     1741 gcttattaaa agtttcgctc tggacgatca atatggtatt gctttagagg tggaagtagc
     1801 taattattta gttaccaatg gcatagaatt agattttgga agcggtattg ccgatactga
     1861 aaaaactact aaaagcaaac tgcaggatta tcggtttatg ttttatgccg ataatgagat
     1921 ttttaaaaca acattagcaa aaatgaagaa aaaacagccc tctggtactt tcaatagttt
     1981 caactggatg gctttacgcg gtaaatattt tactcttgtg ttaaaagaaa acgaacctgc
     2041 tctgatgcgt tcgtatacca gcactttaaa tcccgaaact ggcaatcctg cttttgtgat
     2101 agattccaga caaagtaatc ccaaacgaag ctggaagcag agttttatta tatatgcagg
     2161 ccctgcggac tatcaaatct tgcaaaaata cgggaagcaa atggatagca ttccagaaag
     2221 aggtgcatcc tggttacgct ggctttccaa tatttttgct ggtctactga aatttttgca
     2281 tcgctacatc aaaaattacg gagtggtaat tcttatcttt gctcttatca taaagttatt
     2341 actgcatcca ttaactcagc atcaaatggt gcacagtatg cgtcagcaaa aaatgcagcc
     2401 ctatattgag gaaatccgta aaaaatatgc caatgatgta gtaaaacaac gcgaagaact
     2461 aaccaagctc tataaagaaa ataaaaccag tatgacagct ggttgcttgc ctttactaat
     2521 tcaaatgccg attttaattc cgctttacag tgttttgcgt tattctctgg atatgcgaaa
     2581 cgcttctttt tgtttgtggc tgaaagacct ttctgaacct gatccctata tgattctgcc
     2641 tattattatg ggtggattta tgctcttaca atctttgatg atgcgtccgc gcaatgtaga
     2701 tacttcgcaa atgacggaac agcaaaaaat gcagcagtcc caaatgaaaa ttatgacctg
     2761 gggtatgccg atttttatga ttatcatctt tcggaatatg cctgcaggat tggttttata
     2821 ctggactacc ttcaatgtgc tgtctataat tcatcaatat tttttaacta aacacctcaa
     2881 gaaaaaggaa aatcaatgac aactatagat aaaagcggcg aatccctgga aaaaataatc
     2941 aaggatttcc gcaatgaata taagatccac gattgggagc tgcaatacga aatcctgaaa
     3001 aaaccctcaa aagggatttt tgggttgttt gcccataagc ttgctctcgt gcgttttcaa
     3061 ctaccttctt ctttggatag agctaagctc tttatccaaa acttgctgga aaaaatgagg
     3121 attaaataca cctctttgga gagcagaatg gaaggtaaaa caatttattt ggacatcaat
     3181 ggctcccccg attctggctt tttgattggg aaaaacggct ctatgctgga taatttgcaa
     3241 tatttattaa atcgtgtttt tgaaaatgac cgccaggtag agagaattta tctggatgta
     3301 gatggttata gagaacgcca ggcagataat tttctgaaac cctatctttc tcaaatcgta
     3361 aatgttaagc ttagcgataa acccgtaacc ctggaaccta tgaatgctgc agaacgcaga
     3421 attattcatc gatttgtgga aaaggaaaat ggtttgcgca cccttactat cggtgaagga
     3481 gaaagaaaac ggatagtgat cttctctgcc aaacaaagtg aaaaagaagt tctctccaaa
     3541 agcaaaaagt atagccaaca tcgctctaaa agcactgatc cacaaaccaa aaataaaagc
     3601 ggtaaaccta aatctactcc aggaaaagga aacccgaaac cccatataaa acaacctcaa
     3661 atgagtaacc caaaacacca atgaaacaaa tctatcctgt tcctgcaggt ttttattggt
     3721 ctgatagcgg tgcctttatg ggtgttttgc cctatgcgct ttggagcaaa aaatccgaaa
     3781 tcgatgaacg ctttcgccga aaactgaatt taaacctact cttgattcaa agcggaaacc
     3841 gcaatatttt aatcgatacg ggtttgggaa atcgcctttc cgctaaacaa agagagatct
     3901 atcaaccaag tgaatttcta ttgcctgttt cgcttgcgga attgggtctg caagataaag
     3961 atataacaga tgtaattatg actcatctgc attttgatca cgccggcgga attgttaccg
     4021 attttggaac ttatgaagca ttaactttcc ccaaagcaca ctactggatt caaaaagcgg
     4081 aatgggaaat ggctaaaaat cccgatgaac taaaccaggc agcttataac tatgaacagc
     4141 aattatcgct tctggaacaa aaggggaata ttactctcct tgaaggtgaa gcagaaattg
     4201 aaccggggat tactttaacc ctggttggag gccacactgt cggtagccaa attattacta
     4261 ttaactttgg tacggagtat tttatatatg ctggagatat tattcctact atgtttcata
     4321 cttctttggc gataacctcc gcttatgatg tttcccgcat tcaaaccgtt gctgccaaaa
     4381 aacttatcta tagtcggtta caggaacata acggaatttt actcctaaac cacgacctct
     4441 ctcattggca agttcccttt tcggaattga aaattggcaa gaaagaaaaa aaggataaaa
     4501 tggatagtta gttggaacaa taaaggaagt gagaaattac catactttgg tttacatagc
     4561 tgccaataat ctaaccaaaa ccattgtctt tccgttcttt tcctactttt tccagctgtg
     4621 gaagctgcag aaaaaaagag aggtgataaa atgttcttta caaaccgcat tattaagatt
     4681 gtatcttttt gcctggtagg attattactt tcttcctgcg taaaatatag gggtatcgaa
     4741 gttccagtaa atcctcctcc ctcttttgaa aaaaactata tcttgctgat ctggacacaa
     4801 aaaggcaaat ccaaaacaaa tcctaaaaag agtactttac aagccaacga actctttatg
     4861 aaaaatataa ctattgcagg agacacttta acgggatttg tgtataacaa acctaataaa
     4921 aagcatataa agaaccagat aagtgtttat attgatgatt caattgccca accagattcg
     4981 attcccagcc cattttcttt acctgtttct gccatccaaa aaatagaaac ttatgaagtg
     5041 aatgtgggaa caactattct taccagtatc cttggtattg ctacgacaac tttaaccata
     5101 gcaatcattt gtgttgggta tttaataatc tatcaaattg tcactcaata cgattaatat
     5161 agagagtata gcagagggct tgcgccaccg tcgctataat tgtatcggcc tttgagttaa
     5221 aatcggcttt ctatgtgatc tgccacctca tttcattctt ttttcctatt tttgaataag
     5281 gtcattaata atctcataga aaaggccgat tttgacttaa agccgactaa tcgtcattta
     5341 taaaatacaa tcctcttaat cctaaaatcc ttaaatcctt gtttctaaat tttaatcctt
     5401 tgaatcctat tcatcctatg cctattatca ttttcgaggg gcttacgcca ccgtcgctat
     5461 gatcgtgtcg ccctaagggg cttt
//
LOCUS       DAOSTH010000098         5342 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_14020_length_5342_cov_11.450539, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000098 DAOSTH010000000
VERSION     DAOSTH010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14020_length_5342_cov_11.450539"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="PLB65_07695"
     CDS             complement(<1..230)
                     /locus_tag="PLB65_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78447.1"
                     /translation="MGIHPSGKGWTLFQGRKVFIPNTITGEIISGEIVYGGSEFLSGI
                     LIEIEKQHPYRREPVCKHFKLCGGCLWQHIQYP"
     gene            complement(376..669)
                     /locus_tag="PLB65_07700"
     CDS             complement(376..669)
                     /locus_tag="PLB65_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78448.1"
                     /translation="MKAVFIVFNQAITDKVENALELAQIKGFSKWNEMAGSGSNEGEP
                     HLGSHTWPALNSGILIVLEDEKVKTLLNLVEQINKIAETQGIHAFVWNIETMV"
     gene            complement(739..3882)
                     /locus_tag="PLB65_07705"
     CDS             complement(739..3882)
                     /locus_tag="PLB65_07705"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOQ78449.1"
                     /translation="MSIYSESVRKPVTTIMIFVAVIVLGVFSLVNLPIDLMPDMDIPA
                     MSVITFYPGASAEDIEVNVTKPLENQLNSLQNLKKVTSTSKDNISFILIEFEYGTNMD
                     QSSNDVRDLLSLVENTLPEDAEKPTLFKFNSSMIPVVIYGVTAKESYPALEKIIDERI
                     TERLSRIDGVGAVTVMGKPIRIVDVRVDPRKLDSYGLTLEQVGNAIGAENLALPAGRM
                     EVGQMEFPIRTNGEFKNSDVVKDIVVSKSSAGLVYLRDVAQVKDTLKHIYVDERIDGE
                     VGVRLMVQKQSGANTVQIANLVRERMDELSKELPSDVKIVNIIDTSEFIQSSINNLSE
                     TLLYAFIFVTLVVFIFLGRWRSTFIILLTIPVSLIAAFIFLYAIGGTLNIISLSALAI
                     AIGMVVDDAIVVLENITKHIERGSNPMEAAVFATREVGLAVVASTLVIVAVFFPLTLM
                     TGLTGVVFRQLGWIVSITITISVLAALSLTPMLSSRILRSNLYYNYHPNKFLNAVTRF
                     WGRFDDWYAKSLSYALRHKKLILSAGFVLFVGSLFLLNFIGTEFIPEQDNGRIGINIE
                     IPQGVRIDESKKLARDFENVIRQKYPEIELISTSIGIDEEGSMASAIQKAGSYIITMT
                     IRLSSVEERSRSVFEIVDSLRNDLTKYPIIASSYVSVGGSRNQMAATAMGGGGNNVVV
                     NIFGYDINKTNYIADQISSYMKTQPGFRDVTISRDKDRLEIQVIPDRTKLAQLGMNTA
                     MLSSAIRNRVLGFTATKYREEGNEYDVIVKYNDDNLVSIDDIRNITFRGPQGNLVRVN
                     DVAEVREVYTTPNIEHENKTRVVRVEASLSGTDLGKATNMINSRLKTLDIPPEIFVEI
                     SGAAKDMADTFRDLGLLFILIMVLTYIVMASQFESMREPFIIMFSIPFAITGVLIALA
                     ITNTSINIISGIGIVMLVGIAVKNSIVLVDFINLLVSRGYSIKDAIIAGGRSRLRPIL
                     MTSFTTLLGMLPLAISQGEGSEIWRPLGISIIGGLFFSMLISLIIVPVIYSLFAHAKK
                     RREENNKAAQELFLPSE"
     gene            complement(3925..4965)
                     /locus_tag="PLB65_07710"
     CDS             complement(3925..4965)
                     /locus_tag="PLB65_07710"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOQ78450.1"
                     /translation="MKNKNFLAIMLLLNMAFLISCSNSKSSETTVQQTPNVVTSPIEL
                     TSIERVIRYPVTILAYKENHLAPATAGRVEKILVDIGDQVKTGQVVAQLDRTNYQQAY
                     IQYQKIHLDLMRMDSLLKVGAIAPQQYDQVKMQYDVAKTNLEFLEDNTTLKSPIDGEV
                     TGKYLNDGEMFTMSPAPGIGKPAIVSIMQLNRLKMLVGVSSKYLPNIKRGMKVDITSE
                     IYPGKVFTGSIDKIYPTVDNMSKTFTVEVIINNQGHILRPGLFATASIDIGKDEALVI
                     PSFIIQKQSGTNERFVFIYEDGKAIRKSIEVGDVFDDKVEIVSGLNPGDELIIEGQMA
                     VKDGQSVNRVSK"
     gene            complement(5006..>5342)
                     /locus_tag="PLB65_07715"
     CDS             complement(5006..>5342)
                     /locus_tag="PLB65_07715"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOQ78451.1"
                     /translation="MRRQLSQAEIGLRQLDLQEAYTRNNLEAQIQNAKNAIYTAIKKV
                     DAASGNVELSQKGYKIAQTRYNTGQATLVELNDAENAMMQARLNLIQARSEYLNARNE
                     YQKIIGKTM"
BASE COUNT         1570 a         1086 c          954 g         1732 t
ORIGIN      
        1 ggatattgaa tatgctgcca taaacatccg ccacaaagct tgaaatgttt acaaacaggt
       61 tctcgcctgt aggggtgctg tttttctatt tctattagaa ttccgcttaa aaattccgaa
      121 cctccatata caatctctcc cgaaatgatt tcgccagtta tggtgtttgg gataaaaacc
      181 ttcctccctt gaaataatgt ccaacctttt cctgaaggat gtatgcccat tatttccaaa
      241 tggcgtgcaa tttttcgttt cttctgttta cggctcatgg aatttcaaaa atataacaaa
      301 ccaccttagc atgtatagct ataggtggtt tgttagaatg ttgataaagt ttagtttgat
      361 ttgttaacta aaccactaaa ccatagtttc tatattccag acgaaggcat gtataccttg
      421 agtttctgct attttattga tttgttcgac taaattgagc agagttttaa ctttttcgtc
      481 ttctaaaacg ataagaatac ctgaatttag ggccggccaa gtatgagaac ccaagtgagg
      541 ctcaccttca ttagaacccg agccagccat ttcgttccat ttactaaagc ctttaatttg
      601 agctaattct aaggcattct ccactttatc ggtaattgcc tgattaaaaa cgataaatac
      661 tgctttcatt ttttttattt tttaatgaga aaaggtgtca gtgctcgaaa aataagcacc
      721 aacacctgaa attagacttt attcggaagg gaggaataat tcctgggcgg ctttattgtt
      781 ttcttctctt ctctttttcg catgagcaaa taaactgtaa attactggca cgatgatcag
      841 agagataagc atagagaaaa acaatcctcc gataatagaa attcctaatg gacgccagat
      901 ttcagaaccc tcaccttgag aaatagcgag aggaagcata cccagaaggg tggtaaatga
      961 agtcattaga ataggtctta accttgatct tccaccagct ataatggcgt cttttatgga
     1021 atatcctctg ctaacgagca aattgatgaa atcgaccaat acgatggagt ttttcactgc
     1081 aattccgact agcattacta tcccaatacc cgaaatgata ttgatactgg tgtttgtaat
     1141 ggccaatgcg attaatacac ctgtaatagc aaatggtatt gaaaacatga taataaaagg
     1201 ttcgcgcatc gactcaaact gagaggccat tacaatatat gtgagcacca tgataagaat
     1261 gaacaaaagc cccaaatctc taaaagtatc ggccatatct ttggcggcac cacttatttc
     1321 gacaaaaatc tcaggaggga tgtcgagagt cttcagtcga gaattgatca tattggtagc
     1381 ttttccaagg tcagtgccac tgagcgaggc ttcgactcta actacacggg ttttattttc
     1441 atgttcgata tttggtgtag tataaacctc cctaacttcg gctacgtcgt ttactctgac
     1501 caaattgccc tggggtcctc taaaagtgat atttcgtata tcatcgatcg aaaccaggtt
     1561 atcatcgtta tatttaacaa taacatcgta ttcatttcct tcttctctat attttgtagc
     1621 cgtaaatcct aacaccctat ttctaatagc gctcgataac atggctgtat tcatacccaa
     1681 ttgggccagt ttagttcgat cgggtattac ctggatttcg aggcggtcct tatcgcggga
     1741 aatggtaacg tctctgaaac cgggctgtgt tttcatatag cttgagattt gatcggctat
     1801 atagttagtt ttattaatat cgtagccaaa aatatttacc actacattat tacctccacc
     1861 acccatagct gtggctgcca tttgatttct tgaaccacca acactaacat aagaagaagc
     1921 tatgatggga tatttggtta aatcgttgcg caaactatcg actatttcaa aaacactacg
     1981 tgaacgttct tctacactac ttaatctaat ggtcatggta ataatgtaag aaccagcctt
     2041 ctgaatggca ctggccatag aaccttcttc atctatgccg atactggttg aaattaattc
     2101 gatttcgggg tatttttgtc ttataacatt ctcaaaatcc cgagctagct tcttagattc
     2161 atcaattcta actccctgag gaatttcaat attgatacct attctgccat tatcttgttc
     2221 aggtataaat tcggttccaa taaaattgag taagaataag cttccaacaa agagaacaaa
     2281 tccagcagaa aggataagtt ttttatgtcg aagggcataa cttagagatt ttgcatacca
     2341 atcgtcgaac ctcccccaaa atcgtgttac agcatttaaa aatttattag gatggtagtt
     2401 ataatagagg ttgcttctca aaatacgaga agaaagcatg ggagttagcg ataatgcggc
     2461 taatactgaa attgtgatag ttatgctgac tatccaaccc aattgtctga atacgactcc
     2521 agtaaggcct gtcattaaag tcaatgggaa aaatactgct acaataacta aggtacttgc
     2581 tacaacggct aaacctactt ctcttgttgc aaatactgcc gcttccatag gattactacc
     2641 tcgctctata tgtttagtaa tgttttccaa cactacaata gcatcatcca caaccatacc
     2701 tatggcaatg gcaagtgcag aaagtgaaat gatattcagt gttccaccga tggcgtaaag
     2761 gaagataaat gctgcgataa gtgaaactgg aatagtgagt aggatgatga aggttgaacg
     2821 ccatctgccc aagaaaataa atacaactaa agtaacaaaa ataaaggcat ataaaagagt
     2881 ttcggaaaga ttgttgatag aactctgaat gaattcggat gtatcgatga tatttactat
     2941 ttttacatca ctagggagtt ccttgctgag ttcatccatt ctttcacgca ccaaattagc
     3001 aatttgaacg gtatttgctc cactttgctt ctgtaccatc aatcttactc caacttcccc
     3061 atcgatgcgt tcatctacat aaatatgttt aagtgtatcc ttaacttggg caacatctct
     3121 taaatacact aaacccgcac tcgatttcga aaccacaata tctttcacaa catcgctgtt
     3181 tttgaattca ccattggttc taattgggaa ctccatttgt ccgacttcca ttcttccggc
     3241 tggtaatgca agattctctg caccaatcgc attacctacc tgctcgaggg taagaccata
     3301 agaatctaat ttgcgaggat caaccctcac atctacaata cgtatgggtt ttcccattac
     3361 ggttactgca cctaccccgt ctatacgact taatctttca gtaattcgtt catcaatgat
     3421 tttttctagc gcaggataac tttcttttgc tgtaaccccg taaattacta caggtatcat
     3481 cgaagaattg aatttgaaca aggtaggctt ttcagcgtct tcgggtaaag tgttctctac
     3541 caaactaagt aaatctctta catcattact cgactgatcc atgttagtac catattcaaa
     3601 ttcaattagt atgaacgaaa tattatcctt actggtagaa gttacctttt tcaagttttg
     3661 aagtgaattt aattgatttt caagtggttt tgtcacattc acctctatat cttctgcact
     3721 ggctcctggg taaaaagtaa ttacactcat cgccggaata tccatgtcag gcattaaatc
     3781 aatgggtaaa ttcacaagtg agaatactcc caatacgata acagccacaa aaatcatgat
     3841 ggtggttacc ggctttctta cagattcact atatatactc atattttttt cctatttaaa
     3901 aagtttagct tatctggtca atgcttactt cgacacacga ttgacactct gtccatcttt
     3961 tacagccatt tgaccttcga taattaattc atctcctgga ttcaatccac ttacaatttc
     4021 aactttatca tcaaaaacat cacccacttc tatcgactta cgtattgctt taccatcttc
     4081 ataaataaac acaaagcgtt cgttggttcc cgattgtttt tgaatgataa atgatggtat
     4141 aaccaaagct tcgtcttttc caatatctat ggaggcagtg gcaaataaac caggtctgag
     4201 aatgtggcct tgattattga tgattacctc aaccgtaaat gtcttactca tattatcaac
     4261 tgtaggatat attttgtcga tgcttccagt aaaaacttta ccaggataaa tctcggaagt
     4321 tatatctact ttcatacctc ttttaatgtt gggaagatac ttcgaggaca cgcctaccag
     4381 cattttcaat cgatttaact gcatgatgga tacaatggca ggtttaccga tgccaggagc
     4441 cggactcatg gtgaacatct ctccgtcgtt aaggtactta cctgtaactt ctccgtcaat
     4501 gggagatttc aaagtggtgt tatcttctaa aaattcgaga ttggtcttgg ctacgtcata
     4561 ttgcatcttc acctgatcgt attgttgagg agcaatagcg ccaactttca gaagcgagtc
     4621 catgcgcatt aaatctaaat gtattttttg gtattgaata taggcttgtt gataatttgt
     4681 tctgtcgagc tgggctacta cctgcccagt ttttacctga tcgccaatat ctacaagaat
     4741 tttttctact ctcccagcag tcgctggagc aagatgattc tccttatagg caagtatagt
     4801 aacaggatac ctgataaccc tctcgatgct ggtgagctca atgggtgatg tcaccacatt
     4861 aggagtttgt tgaactgtgg tttcggagct tttactattg ctgcatgaaa tcaaaaaggc
     4921 catgttcagg agcagcatta ttgctaagaa atttttattt ttcatagtta tttgtatgtt
     4981 ttattttaaa atgattcttg ttgaattaca ttgttttgcc aataatcttt tgatattcat
     5041 tgcgggcatt tagatattcc gaacgagctt gaatgaggtt gagtctagct tgcatcatag
     5101 cattttcggc atcatttagc tcaaccaggg tggcttgccc agtattatat cgtgtttgtg
     5161 caatcttata tcctttttgt gaaagttcta cattacctga ggcggcatct actttcttta
     5221 tagctgtgta aatggcattc ttcgcatttt gtatttgggc ctcaagatta ttgcgtgtat
     5281 aagcttcttg aagatcgagc tgacgcaaac ctatttcggc ttggctcagt tgacggcgca
     5341 tc
//
LOCUS       DAOSTH010000099         5312 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_14129_length_5312_cov_11.691079, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000099 DAOSTH010000000
VERSION     DAOSTH010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14129_length_5312_cov_11.691079"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLB65_07720"
     CDS             complement(<1..465)
                     /locus_tag="PLB65_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78452.1"
                     /translation="MKKNILLILCVLAAAILPGQKMVLRNIQQYHKLTDHPYQNKLIQ
                     HNSKQDLTKRDNANYNRLLVILVDFALEETDDTNTTGNGKFQLQPDSTYIYSVGAPPH
                     NREYFEANLEALKYYYLAVSAGDYTLNYDVYPKNKPAYTLPKSMGYYNPPGAS"
     gene            complement(532..1512)
                     /locus_tag="PLB65_07725"
     CDS             complement(532..1512)
                     /locus_tag="PLB65_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018196970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HOQ78453.1"
                     /translation="MFKRHAIIFSILLMLILPYALSGISQATMLFLTLEPGARANAMG
                     RAYSAIADDAYAMWWNPGAAAFNKDTQIAATHIPWLQGSGIDDMFYEYLGFNNYFYGI
                     GNINAHIIWLDAGTQPHTSSQNVDLGDFHTYEVAGAIGYGYEIVPEKIGIGGNFKLLY
                     SYLGPGTGLTETEGSAFSFAFDAGALFKDVAVDNLNASIVIQNIGPDITYVDKDQSDP
                     APMTFRAGLGYTVFDNPMGCLITTAEMSKILADDDPIYGVGAEYTYLNLIALRGGYYS
                     DNVGEVEGASFGVGVHYKFSNRYKLGFDFAMVPAGGLTKYNKIFSLGFEF"
     gene            complement(1708..1794)
                     /locus_tag="PLB65_07730"
     tRNA            complement(1708..1794)
                     /locus_tag="PLB65_07730"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1758..1760),aa:Leu,seq:caa)
     gene            2046..3155
                     /locus_tag="PLB65_07735"
     CDS             2046..3155
                     /locus_tag="PLB65_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ78454.1"
                     /translation="MLTKLWVNNYRCLVNFEIEFDSLTLLLGPNGGGKTSIFDILYNL
                     RRLIIDNAKVYEVFFPEDLTAWLDSNEQTFELQVEGNGGIYTYRLVISHQRELGKLRV
                     ERETLHYDHKVIFSFEKGDIHLYNDYFKEGAVISFDWMFSGLSMVVPRQDNTKLTWFK
                     DWINKLFILNLEPKAIHSVSEDESSQLFRDGKNFVSWYRYFSQEHQDKMYDLITYLRN
                     SIYGFDSLKLELAGKHRILQVGFRSENKKDKTLFFDFDRLSDGQKVLLILHTLLVCLQ
                     DMGNTLMMDEPENYVALAEIQPWLMELSDVCGVTIPQIILISHHPELIDYFGVEKSRL
                     IVREPLGPARVKKIFNRFEDSLKLSELIARGWENE"
     gene            3148..3705
                     /locus_tag="PLB65_07740"
     CDS             3148..3705
                     /locus_tag="PLB65_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78455.1"
                     /translation="MSKRKVSLVICCEDKQQECFARRFLKGLGWDYRQLFFRINSAGK
                     GSGEQWVREKFVEELNIYRSKQSTNAFIAIIDGDTKGVEARITQFNNICREKGVQVRS
                     NNDAVAIIVPTRNIETWIHYLNGETVDEETVYPKLKFESECQSAIDNLVTMCKGNGLN
                     QDAPPSLHSACKEYRNRIYNFRFAH"
     gene            complement(4760..>5312)
                     /locus_tag="PLB65_07745"
     CDS             complement(4760..>5312)
                     /locus_tag="PLB65_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOQ78456.1"
                     /translation="ITWVTQSETNIMGYYIYRSPQADLNTAINLNYLISATNSSQLQI
                     YTYKDQVPANNIYYYWLEVVDYDGYNSFYGPSIVEVNDNGNQTPSTPVKAGFYSSSPN
                     PFDSQTKIFYGIPSKSPVLMQVYNIKGQKIRTLVNETKDTGNFDLFWDGRDENGNLLA
                     NGNYILHLAAAGNSWTKKITILH"
BASE COUNT         1680 a          912 c         1028 g         1692 t
ORIGIN      
        1 actggctccc ggaggattat agtaacccat actttttggc aaagtatagg caggtttatt
       61 tttaggataa acatcgtaat taagagtata atcgccggca gaaacagcta aatagtaata
      121 tttcaatgct tccagatttg cctcaaaata ttctctgtta tggggtggag caccaacaga
      181 ataaatatag gtggaatctg gttgcaattg gaattttccg ttaccggtag tattggtatc
      241 atcggtttct tctaaggcaa aatctaccag gataaccaat aaacgattat aatttgcatt
      301 atccctttta gttaaatcct gtttggaatt atgctgaatt agcttatttt gatatgggtg
      361 atcggtaagt ttatggtatt gttggatgtt ccgcaaaacc attttttgtc ctggtaagat
      421 agctgctgcc aagacacata agatcagtaa tatattcttt ttcatcttac ttatgtgaag
      481 ctattgttcc ttcatcttct atagtgcaag gagggcatca ttctcccggt tttaaaactc
      541 aaaccccaga gagaaaatct tattatattt tgttaatcct cctgcaggaa ccatagcaaa
      601 atcaaatccc agtttataac gattgctgaa tttgtaatgc actccaacac caaaactggc
      661 gccttccact tcccctacgt tatcggaata ataaccgccg cgtaaagcaa tcagatttaa
      721 gtaagtatat tctgctccaa ctccataaat gggatcatcg tctgccagaa ttttactcat
      781 ttcggcagtt gtgattaaac atcccatcgg gttgtcaaaa actgtatagc caagcccagc
      841 tctgaaggtc atcggagcag ggtctgattg atctttatcc acataagtaa tatcgggtcc
      901 gatattttgg ataacgattg aagcattcag attatctaca gccacatctt tgaataaggc
      961 acctgcgtca aaagcgaaac tgaaagcaga accttcggtt tcagttaaac cagtgccggg
     1021 tcccagataa gaatataata gcttgaaatt gcctccgata ccaattttct cagggactat
     1081 ttcgtagcca taacctatag ctccggcaac ttcataagtg tgaaaatcac ctaaatctac
     1141 attctgggaa ctagtatggg gctgagttcc ggcatccaac caaattatat gagcattgat
     1201 gttaccaata ccataaaagt aattattgaa tcccaaatat tcatagaaca tatcatcaat
     1261 cccagaacct tgtaaccaag ggatgtgagt tgcggctatt tgtgtatctt tgttaaaagc
     1321 tgcagccccg ggattccacc acatagcata agcatcatct gcaatggcag aataggcacg
     1381 gcccatagca ttagcacgag ctccgggttc taaggttaag aataacattg ttgcctgaga
     1441 aatgccagac aaggcataag gcagaattag catcagcaaa atgctgaaga taattgcgtg
     1501 gcgcttaaac attatacctc cacagggttt tgtataaaag tttcaggttg catataaaga
     1561 tcaaccgaag tatatgatat gaatttatat aatctaatct gcgggaaata aatatcagtt
     1621 tagattatgc ctcttctaca gtcaactaca ttattaggac ttagtcccca tagcaaataa
     1681 aaggtcttcg ctcctcaaag ttaacgatgg tgccgaaggc cggaatcgaa ccggcatgag
     1741 ctaatgcccg gtggattttg agtccactgc gtctaccagt ttcaccactt cggcactctc
     1801 tttattttgc cacaaaatca agcactgttt ccctgtcaag taaaaaggca tttttgctct
     1861 cttcggaaca ttttatcttt aataacaata taagccatca accgcaaaaa gcaaagttat
     1921 ccaattggcg ttagcttgag aggagagaat tgctttgaca taaatactta acttaattat
     1981 ttggtttgaa agataattgt atatcaggat tcagatttat gaattgctta agtaaggagt
     2041 ataaaatgct aactaaactt tgggttaata attatcgctg tttagtcaat tttgagattg
     2101 aatttgatag tttaacttta ttacttggtc ccaatggcgg gggtaaaaca agtatatttg
     2161 atattctgta taacttaaga agattaataa tagataatgc caaggtctat gaggtctttt
     2221 ttcctgagga tttaaccgct tggcttgatt ccaatgagca aacatttgag ctacaagttg
     2281 agggtaacgg aggaatttat acctatcgtt tagtcatttc tcatcaaagg gagttaggga
     2341 aattacgcgt tgaacgggaa acacttcatt atgatcataa agtaatcttc tcttttgaaa
     2401 aaggtgatat tcatttatac aatgattatt ttaaggaagg agcggtcatt tcttttgatt
     2461 ggatgttctc aggactttct atggttgtcc ccaggcagga taatacaaaa ttaacttggt
     2521 tcaaggactg gataaacaag ttatttattt taaatctgga accgaaagcc atccattctg
     2581 tttctgaaga tgaatcctca cagttattta gggatgggaa gaattttgtt tcctggtatc
     2641 gttatttttc tcaagagcat caggataaaa tgtatgattt gataacttat ctacggaatt
     2701 ctatctatgg atttgattcc ttaaaattgg aattagcagg taaacatcgt atcttgcaag
     2761 ttggctttcg gagtgaaaat aaaaaagata agaccctatt ttttgatttt gatcggcttt
     2821 ccgatgggca aaaagttttg ctcatcttac atacattgtt agtatgttta caagatatgg
     2881 gtaatacatt gatgatggat gaaccggaaa attatgtagc acttgcggaa attcaaccct
     2941 ggcttatgga acttagtgat gtttgtggag ttacgatacc ccaaattata cttatcagtc
     3001 atcatcctga actgattgat tattttggag tggaaaaaag ccgtctgata gttagagagc
     3061 cactgggtcc tgcaagagta aaaaaaatct ttaacaggtt tgaagatagt ttaaaattat
     3121 cagaattaat tgcccggggt tgggaaaatg agtaaaagga aagtaagctt agttatatgt
     3181 tgtgaagata aacaacaaga atgttttgcc agaagatttc tcaaaggttt gggttgggat
     3241 tatagacagt tattttttag aataaactct gctggaaaag gttcaggaga acaatgggtt
     3301 agagaaaagt tcgttgaaga attaaatatc tatcgttcta agcaatctac taatgctttc
     3361 atagctatta ttgatggaga cacaaaaggt gttgaagcaa gaattactca atttaataat
     3421 atatgccggg aaaagggagt tcaagttcga agtaataatg atgccgtagc cataatagtt
     3481 ccgaccagaa atatagagac ctggatacac tatttaaatg gagaaacagt tgatgaagaa
     3541 acagtatatc ctaagctgaa atttgaaagt gaatgccaat ctgcaataga taatcttgtt
     3601 acaatgtgta aaggtaatgg acttaatcag gatgctccac cttcactaca ttcagcttgt
     3661 aaagaatata gaaatagaat ttataatttc aggtttgcac attagtatgt ttaaaactga
     3721 ttattatgga taatgaccct taatgaagat ttccgttttc cccattttaa aacagggtag
     3781 tatcaacttt atgctcttta ccctctgaca aaaaaccttt aggccaaccc aatgataaca
     3841 atggtggtgt aagtttctcg taaaattcac gatgcaaccg gcaggatgtt ttgacttaca
     3901 attttcacct gccttcaatg taactacacc ccacactttt agttcactta caaccgctct
     3961 gtagcttgtt tccaaaccgg aaaattgtga gttaaaacaa aaagcacaca cccttccaac
     4021 ccaactatca atcaccctct atttacacag catagaacaa caacaccata atgcgataac
     4081 tcactatcag caatgattat tctttttccc ttcccaagat atttgtttat caattcctgt
     4141 ttttttgtgc tatgcagcta atgagattcc cgtatcgttc ccatattgcg atacgagaac
     4201 gatacgggag tcatatcgga gagttattga gaaaattcag ggttctcttt ggcagtgatt
     4261 tgtagtcaat ttgctaatgg caaaccgtgt gttgatttag tggtttattt cggtaaaatg
     4321 agaaagcgcc gactcttttt tttcaaataa ggtatggatt aaggtttatt ttgccagcta
     4381 ttatgtcaga tttcgggtta tttggtttta tcgctttgaa gggttgttta tctatacagt
     4441 ttaaagaggt tgcttgattg tttggttgta aggacgcata ctttctgttt taactcacaa
     4501 tttttcggtt tggaaaccag tagtagaata gtcggaagtg agaaaagaag gtgaggtgtg
     4561 attacattga aggcaggtga aaattgtaag tcaaaacctt ctgccgcttg catcgtgaaa
     4621 aaaggtaatt atttaattct ttcctaaatt aactactgac accttaccta cccattgagg
     4681 acggcatccg taacaggtga gcatttagtt ggtgaaacta ttaaggaagg caaaagttat
     4741 accggagtat aatattgtat tagtgtaaga tagtaatttt ttttgtccaa gaatttcctg
     4801 cagcagcaag atgtaagata taatttccat tggccaacaa atttccattt tcatctctac
     4861 catcccagaa aagatcaaaa ttacctgtat ccttggtctc attcacaagg gttcttattt
     4921 tttgcccctt tatattgtaa acttgcatta aaacagggga ttttgaggga atgccataga
     4981 aaattttagt ttgcgaatca aaaggattgg gagaagatga atagaatcct gccttaacag
     5041 gtgtggaagg tgtttgattt ccattatcat ttacttctac aatagacggt ccgtaaaatg
     5101 aattatatcc atcgtaatca acaacttcca gccagtaata gtaaatatta ttagcaggaa
     5161 cctggtcttt ataagtgtat atttgtaatt gggaagaatt cgttgctgaa ataaggtaat
     5221 tgaggtttat ggctgtattt aaatctgcct gtggggaacg gtaaatataa tatcccataa
     5281 tatttgtttc ggattgagtt acccaagtta tt
//
LOCUS       DAOSTH010000100         5293 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_14210_length_5293_cov_13.289996, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000100 DAOSTH010000000
VERSION     DAOSTH010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14210_length_5293_cov_13.289996"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..1671
                     /locus_tag="PLB65_07750"
     CDS             76..1671
                     /locus_tag="PLB65_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78457.1"
                     /translation="MQKNMKEALFLDKLFVLLETQGEVMEAVKHQKLNKKHYSFEELT
                     AHNRKTENLLKAISKNKALEAKLEGIFHELSVLPLKHIHTNERLDLNELFEIKSFLFY
                     YLHLKQTLQENKLDCLHPLPDMQNLFTLLDPEGNNLPTFRIYPSYSPLLIKLTQRQFA
                     IANRLKEVRNRDLENARKELGYPTLKEEFTLSRNRKEQLEAILASQYFIVVSEGLANY
                     NFRLADSKEAAELKTELHQLSILLTEEENRILSSLTAQIQKDFNLLEQAYNGASEYAW
                     NFCLAKFALQYDCCLPVLTNNSEGISISQAVNLPLKLFLENIKRKYQPLEIKLSRKDN
                     LITGPNMGGKSSALITIGQICTLASLGIPLPAKNAKLPIYDEVYYNHDTGENSETLSS
                     FAREVVSLSTMLQHKGKKLILLDEFAKGTNPEEGEAICVATLKYLINSEHTLISATHF
                     SAPTELSGLSHFCIKGIDEKSLKRLEQMKDSSLETRLALLSEAMDYSLMPVSENTNPP
                     KCAIRIAGILGLPQEILEMLAKE"
     gene            1673..2464
                     /locus_tag="PLB65_07755"
     CDS             1673..2464
                     /locus_tag="PLB65_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOQ78458.1"
                     /translation="MQKIKPQQVIDLAIEAGRIILQSGGTTNRVELMMRKVCNCFGYP
                     KTESYVTPTGIFISVKDEYGSIFTSIKRIDNRRIDLGKITSISRMVNCMQLKPPSEQD
                     YNEQLIDFEEELKKIDTENPWPSWLKDLCGGGTSGFFCLLFGGSWAEFAVAFTIGVIV
                     TFGLKYLEKLAFNNFLLNAIAAALIVLLAKTIDIFVPYIRLDNIIIGGIMLLVPGLSI
                     TNAMRDTMSGDLVAGTARAVEAFFITVGIVAGSASMLKILSLWGY"
     gene            2464..2916
                     /locus_tag="PLB65_07760"
     CDS             2464..2916
                     /locus_tag="PLB65_07760"
                     /inference="COORDINATES: protein motif:HMM:NF018448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HOQ78459.1"
                     /translation="MKPFDGLTLMQFLWAFLAILGFSIRSNLKGIKILFTALSGGLCW
                     FFYLIVLYYSKSVLFSVFIAIIVVCIYSEILAPRLKTPVSVFVTCAIIPLVPGRGLFN
                     SMQLYIAGDNLNASKTILQTLLIAGTISIAIAIVSSVTNLINKLMNRF"
     gene            2929..3735
                     /locus_tag="PLB65_07765"
     CDS             2929..3735
                     /locus_tag="PLB65_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HOQ78460.1"
                     /translation="MYKSVKQQLLEQRILKLLSGKALKTAEWLFANPELQAAQEYANI
                     VSIKRLGYNDHGPVHTRKATFNAIKMFKLLNQAEIKMNLEKEGAGTADDSLTAVILAS
                     LLHDLGMAITRDSHELLSIQLALPYIDAVLNTLHSDEEINVRTAIRSLAIEGIFGHMA
                     TRKINSLEAGLVLIGDGSDMEKGRARIPTMLSSKARVGDIHRTSASAIQKVKIFKGQE
                     KPINITVEMNASVGFFQIEEVFFPKINTSPVKPYIELFAGVTDREMLKYL"
     gene            complement(4130..5149)
                     /locus_tag="PLB65_07770"
     CDS             complement(4130..5149)
                     /locus_tag="PLB65_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78461.1"
                     /translation="MKRYLYLALLVGMLFVLNSCITDPPAIPTEEWLCSINADGTNFR
                     LIQKDPNITSGIEEMYSTVDGKLLIYSNAMWLLDPQSLSLVKITADTLINDKICRVSQ
                     LPDGSKMYFSANRNLYELMYPSLHLRQLTSNISESRFKNPVISKSGNILCFSSHTQIN
                     QHKDYTNYGAYINMETQEIVNLPSLGHYLDNLVYNERDKKLYFTISSQLKRCNLDGTD
                     LENVGTGCAETHRFNLSDDQIHLIYDPGVLKIINLLNCSEEIIETGVDMGGHGFFSSS
                     QSGDVIFYVPKYLDKIWKYSVGNHVSELVAGIPSNVSISRIGSLASAWDGSMVYFYCA
                     LKRRK"
BASE COUNT         1655 a          994 c          968 g         1676 t
ORIGIN      
        1 tatagtaagg caccctcaga aaaatgattg acagcagcag caactgatat aaaatgccat
       61 tatgaggttg attttatgca aaagaatatg aaagaagcgc tgtttctgga taaactgttt
      121 gttctgttgg aaactcaggg cgaagttatg gaagccgtaa aacaccagaa acttaataaa
      181 aaacactata gctttgagga gcttacagct cataatcgaa agacggaaaa cttattaaaa
      241 gccatttcta aaaataaagc actggaagca aagttagaag gaattttcca tgaactttcc
      301 gttctgcctc taaagcatat acacactaat gagcgtttag atctaaacga gctttttgaa
      361 ataaaatcct ttcttttcta ttaccttcat ctgaaacaga ccttgcagga aaataaattg
      421 gattgcttac atccgcttcc ggatatgcaa aatctgttta ctttacttga ccctgaaggt
      481 aataatctgc ctacttttcg gatttatcct tcctattcac ctcttttgat aaaacttacc
      541 caaaggcaat ttgccatcgc taaccgctta aaagaagtgc gtaatagaga tttggagaat
      601 gccagaaaag aacttggtta ccctactctg aaagaagaat tcactctttc ccgcaatagg
      661 aaagaacaac tggaagcaat tttggcttct caatatttta ttgtagtttc cgaaggactg
      721 gcaaattaca atttccgttt agctgattct aaagaagctg cagaattgaa aacagaatta
      781 caccaactct ccattctgct tacagaagaa gaaaacagaa ttttaagctc tctaactgca
      841 cagatccaaa aagattttaa tctcctggaa caagcatata acggtgccag tgaatatgcc
      901 tggaattttt gcctggctaa atttgctttg caatatgatt gctgcttgcc ggttttaact
      961 aataactctg aaggaatcag catttctcag gctgtcaatt taccgcttaa gctctttctg
     1021 gagaatataa aacgcaaata tcaacccctg gaaattaaac tctcccgaaa agacaatcta
     1081 attaccggtc ccaatatggg tggaaaaagt agtgctctaa taactatcgg acaaatatgc
     1141 acgctggctt ctttaggaat acccttaccc gctaaaaatg ctaaattacc tatttacgac
     1201 gaagtatatt ataatcatga tacgggtgaa aacagtgaaa cccttagttc ctttgcgcgg
     1261 gaagtggttt cgctatctac gatgttacaa cataaaggca aaaaactcat cttgctggat
     1321 gaatttgcta aaggtactaa tccggaagaa ggagaggcaa tttgtgtagc tacccttaag
     1381 tatttaataa acagtgaaca tactttaatt tctgccacac atttcagtgc tcccactgaa
     1441 ctatccggat tatctcattt ctgcataaaa ggtattgatg agaaaagctt gaaacggctt
     1501 gaacagatga aagatagcag tttagaaact cgtcttgctc ttctttctga agcaatggat
     1561 tattctctga tgcctgtgtc cgaaaataca aatcctccta aatgcgcaat taggattgct
     1621 ggtattttag gcctacctca agaaatattg gaaatgttag ccaaggagta atatgcaaaa
     1681 aattaaaccc caacaagtta tcgacctggc tattgaagca ggacgcatta ttttacaaag
     1741 cggaggaact acaaatcgcg tagaattgat gatgcgtaag gtttgtaact gttttggtta
     1801 tcccaaaaca gaatcctatg ttactccaac gggtatcttt atcagcgtta aagatgaata
     1861 cggcagtatc ttcaccagca tcaaaagaat agataatcgg cgcatagact tgggaaaaat
     1921 aactagcatc agtagaatgg ttaattgtat gcagctaaaa ccaccttcag aacaagatta
     1981 caatgaacag cttatagatt ttgaagaaga actgaaaaaa atagatactg aaaacccctg
     2041 gccttcttgg ttgaaggacc tttgtggagg tggtactagt ggtttttttt gtttgctttt
     2101 tggtggttcc tgggctgaat tcgctgttgc ttttactatt ggagttatcg ttactttcgg
     2161 cttaaaatat ctggaaaaat tggcttttaa taacttcctc ctgaatgcta ttgctgctgc
     2221 tctgattgtt ttactggcaa aaacaataga tatcttcgtg ccttatattc gcttggataa
     2281 tatcatcatt ggaggcatta tgttacttgt tcccggactt tcaattacta atgctatgcg
     2341 cgatacgatg agcggagatt tagtagctgg aacagccaga gctgtagaag ctttttttat
     2401 aacggttgga atcgtggcgg gaagtgcttc tatgcttaaa atcttgtcac tttggggata
     2461 ttaatgaaac cttttgatgg tttaacttta atgcaattcc tttgggcttt cttagctatt
     2521 ttgggcttct ccattcgctc caacctgaaa gggatcaaaa tcttatttac agctttaagt
     2581 ggaggacttt gctggttttt ctatctaatt gtgttatatt atagtaagtc agtattattt
     2641 tccgtgttca ttgccattat tgtagtttgc atttattctg aaatactggc accacggtta
     2701 aaaacaccag tttctgtttt tgtaacttgt gccattattc ctttggttcc aggacgcgga
     2761 ctatttaatt caatgcaact ttatatagct ggtgacaatc taaatgcttc caaaactatt
     2821 ttacaaacct tacttatcgc tggaaccatt tcgatagcta ttgctattgt gtcctccgtt
     2881 acaaatttaa taaacaaact gatgaacaga ttttaggaga aaaacaatat gtataaatca
     2941 gttaaacaac aacttctgga acagcgtatc ttaaaattgc tttccggcaa agcacttaaa
     3001 accgctgaat ggctatttgc caatcctgaa ctacaagcgg ctcaagaata tgctaatata
     3061 gtatctatta agcgtttggg ctataatgat catggccctg ttcatacacg taaagcaact
     3121 tttaatgcga tcaagatgtt caaattgctc aatcaggcag aaataaaaat gaaccttgaa
     3181 aaagaaggtg ctggtactgc agacgattca ctcaccgctg ttattttagc ttcattacta
     3241 catgatctgg gtatggctat tactcgcgat tctcatgaat tgctctctat tcagcttgcc
     3301 ctgccatata ttgacgcagt attaaacact ttacatagtg atgaagaaat aaatgttaga
     3361 accgctatcc gctctcttgc cattgaagga atctttggac atatggccac ccggaaaatt
     3421 aattctctgg aagctggatt agttttaatt ggtgatggta gtgatatgga aaaaggtaga
     3481 gccagaatac ccactatgct ttcttccaaa gctcgcgttg gagatattca tcgcacttcc
     3541 gcttctgcta ttcaaaaagt gaaaatcttt aaaggacagg aaaaaccgat caatataacc
     3601 gtagaaatga atgcctccgt tggctttttt cagatcgaag aagtgttttt ccctaaaata
     3661 aataccagcc ccgtgaaacc ctatattgaa ctttttgcag gagttacaga tagagaaatg
     3721 cttaagtatt tgtaggtctt gagcaaggat aagcgcaggg ttttagaaac ccatatcaaa
     3781 atcccattaa tgcaacagaa tattttatta tgtctcaact cttccgtcgg gtgtttgtag
     3841 atggttatag aatgatgtgc gggttcattg attatatata ggaatagtta ataatgggat
     3901 ccatcgcggc caatacaaga attgaataaa agcacttgtt ttctttttcc ttattttaag
     3961 gtcagtttcg ttgatgaact ttgtccgagt aatgtgttta cctttgcgaa acatacaccg
     4021 cttgccaatt tgttttgcat tgtatctctt ccatcaataa tgttagtgga gtaaatattt
     4081 gtagtgcaaa ataccagtat cgcaatataa taataaactc tatgtttcac tattttctcc
     4141 ttttcaaagc acaatagaaa tataccattg atccatccca agcgcttgct aagctcccta
     4201 ttcgagatat cgacacattg gatgggatac cggcaactaa ttcgcttaca tgattaccaa
     4261 ctgaatattt ccaaatttta tcaaggtatt tgggaacata aaaaatgaca tcacctgatt
     4321 gagaagatga gaaaaatcca tgaccaccca tatcaactcc tgtctcaata atttcctcgc
     4381 tacaattaag taagttgata attttgagca cgcccggatc atagatcaag tgaatttggt
     4441 catctgacaa attaaaccga tgagtttcag cacagccagt acctacattt tcaagatcag
     4501 taccgtcaag attacacctt tttagctgac tagatatagt gaaatacaac tttttgtccc
     4561 tttcgttgta aactaagttg tcaagatagt gtccgagaga tggtagatta acgatttcct
     4621 gagtttccat atttatgtaa gctccatagt ttgtgtaatc tttatgctgg ttgatctggg
     4681 tatgtgaact gaagcacagg atgtttccag attttgaaat cacaggattt ttaaaacgac
     4741 tttcagagat atttgatgtt aattgtctta ggtgcaaaga agggtacata agttcatata
     4801 gattcctatt agcactaaaa tacatcttag agccatcagg taactgtgaa actctgcata
     4861 tcttgtcatt tattaaagta tcagctgtta tcttaactaa gctcaggctt tgcggatcaa
     4921 gcaaccacat agcattggag tagattagaa gttttccatc aactgtggag tacatttctt
     4981 cgatgcctga tgtaatgttt ggatcttttt ggatcaatct aaaatttgtc ccatcggcat
     5041 ttatgctaca caaccattcc tctgttggta tggcaggagg atctgttatg caagaattca
     5101 acacaaacag cattcccacc aataaagcca gatataaata tcttttcatt tcttcctcct
     5161 atctatctga tctttggaat tcttggattt ggtacttact ttgtttcacc agtcctgcat
     5221 taccttcata aacatacatc atagtgtcag gaattgctgt tacatcaatc gtaaacactg
     5281 aatctataac agc
//
LOCUS       DAOSTH010000101         5087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_14947_length_5087_cov_13.939587, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000101 DAOSTH010000000
VERSION     DAOSTH010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5087
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14947_length_5087_cov_13.939587"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            179..1624
                     /locus_tag="PLB65_07775"
     CDS             179..1624
                     /locus_tag="PLB65_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOQ78462.1"
                     /translation="MEDQQNQENQQIIKTRDDPEPVTLEQVNNESSFKIKESKSCSWA
                     KSEDEITPEKLRERIEPWLTALFQSEHLSLLIGSGLTTAVHFLATEKLANGMSKQDFN
                     TFKDEINKSVKDSAEKAGRGEGNFEDQIRVVNELLAGLNILESTKPDENKGKVLEQWK
                     QNHKDLEEDLKSLQQELENALKNFAGSILKSENDLITAKGTKEESLNYLVSFLMSFAS
                     RTGTRDRLNIFTTNYDRYIELGADIAGLRLIDRFVGTISPIFRASRMDIDYHYNPPGI
                     RGEPRYVEGVARYTKLHGSIDWFYSNGEIRRIGLPFGANDITPYLQPCELEKSSAMQL
                     MIYPNPAKDLETLFYPYSELFRDFAAAICRPNSTVVCYGYGFGDEHINRIIKDMLTIP
                     STHLVIISYSDPNDRIMKTYEKLGRNAQITLLIGDHLGDLKTLVEHYLPKPAIDRTTI
                     RMADLIKSRLGAQEQTTQVKTSENDEGGTLP"
     gene            1621..3732
                     /locus_tag="PLB65_07780"
     CDS             1621..3732
                     /locus_tag="PLB65_07780"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ78463.1"
                     /translation="MSQTPFEQASSLVIGTVEFVSPDEIKIAIDIEAPDSMALNTGGP
                     RPFPRVNEYLLIPVEQGFLVGQVEWITIERSPFPKRKGMQDFGLIDLPYPLRRLSLNP
                     LGTLRPLHSGKNFEFTRGCEVLPSVGTPALLPTDEQLKAIIESGEKKRVCIGTSPVAN
                     NAKVYVDPDRLFGRHLAVLGNTGSGKSCTVAGLIRWCIEESKKVNKEVNARFIILDPN
                     DEYSEAFDNLGKVKKFAVKLKEECNEINQLKVPSWMWNSWEFASLSQASEKAQRPLLT
                     RALREIKNGSDFERETKSIQFRRFLTSLKVSLLNYQKSGPKLSEFPIKQNYGGFLEGT
                     QKSLSTFDNNNMTNKEAEIKNELIQQIQRILENKPKDKGYFPPFSLQEVNPLLNILYE
                     FEARVGPLIPYEGPDEDTPIFFKNENFLAHLNEIAQEQSQQQYFDYFIMRIRSLFTDK
                     RIASVISTENCDEVTLLQWLNDYIGQDYNKPSISIIDLSLLPSEVLYTITSVMARIVF
                     EAHHRYKKLNDTVLPTVLVVEEAHNFIKKYETSNDASPQQLCTQIFEKIAKEGRKFGL
                     GLVLSSQRPSELSPTVLSQCNTFLLHRISNDKDQELVHKLVPDNLRGLLRELPSLPSQ
                     HAILLGWASELPVLVKINDLPKLQQPRSDDPDFWNVWTGSDEADNAVERKINWETIAD
                     DWQQKTSSNTNEEKEENEKNE"
     gene            3769..>5087
                     /locus_tag="PLB65_07785"
     CDS             3769..>5087
                     /locus_tag="PLB65_07785"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOQ78464.1"
                     /translation="MNSTTNEILPEGYKMTELGPLPEEWEVVRLGEVADFFLGRTPKR
                     AEKSYWDNGKYSWISIADMKDFGFISKTNGKVSQLAFEKIFKENIVPKGTLIMSFKLT
                     IGRTAILNIDALHNEAIISIYPKGELLKEFLFYFLPTIDFSNYYDNVVKGKTLNSSKI
                     KNLLLPLPPLPEQQKIAAVLSAVQEAKEKTQAVIDATKVLKKSMMKHLFTYGPVPPEE
                     TENVPLKETEIGKVPEDWEVEKLGDLSTKKLIKVQFGFPCGKWNDKGIGIAQVRPFNI
                     NENGNIDLTVLKYIETDNDIGKYLLQKEDIIFNNTNSEELVGKTAFWKDGKIKYVLSN
                     HMTIIRIVDKSKLNAYFLAKYLHKRWNDGFYKTICRRHVNQSSISLARLNDISLPLPP
                     LPVQQKIASILSAIDAKIEAEENKKQALEELFKTLLHNLMTAKIRVN"
BASE COUNT         1797 a          870 c          989 g         1431 t
ORIGIN      
        1 catcaaatgg tatctgctaa aaattaaact aaggaacata tgattatgaa accgtccacc
       61 aacgaaatac tgccacaagg ctacaagatg acagaactcg gaccgctgcc agaagagtgg
      121 tggagtgaga agaaaaatat ttaactttat tatttaaaat ttttggaggg tatttattat
      181 ggaagaccaa caaaaccagg aaaaccaaca aattattaaa acccgggatg accctgaacc
      241 tgtaactctt gaacaggtca ataatgaatc ttctttcaaa ataaaagaaa gtaaaagctg
      301 ttcatgggca aaatctgaag atgaaattac tcccgaaaaa cttagagaac gtatagaacc
      361 ctggcttaca gctctttttc aatcagaaca tttatcatta cttattggaa gcggattgac
      421 cactgctgtt cattttttgg caactgaaaa actggcgaat ggtatgagta aacaggattt
      481 taatactttt aaagatgaaa taaacaaaag tgttaaggac tctgcagaaa aagcaggaag
      541 aggagagggg aattttgaag atcagatacg agtcgtcaat gaattgctgg caggtttgaa
      601 tattcttgaa tcgacaaagc ctgatgaaaa taagggaaaa gtgcttgaac agtggaaaca
      661 aaatcataag gatttggaag aagatcttaa gagtttacaa caagagcttg aaaatgctct
      721 taaaaatttt gcagggtcaa ttcttaaaag tgaaaatgac ctaattactg ctaaaggaac
      781 gaaagaagaa tcgttaaact atcttgtaag ttttctaatg agttttgcaa gtagaacagg
      841 tacacgtgat cgacttaata tttttactac caattatgac agatatattg aattaggagc
      901 tgatattgct ggccttcgac tgatagatag atttgtagga actatttccc caatttttcg
      961 agcttcccga atggatatcg attatcatta taacccacct ggaattcggg gtgagccacg
     1021 ttatgtggaa ggagtcgcgc gttatacaaa actgcatggt tcaatagatt ggttttatag
     1081 caatggtgaa ataaggcgta ttggattgcc ttttggcgct aacgacatta caccctattt
     1141 acaaccttgt gaactggaaa aatcaagtgc aatgcaattg atgatttatc ctaacccggc
     1201 aaaagacctg gagactttat tttatccata cagtgaactt ttccgagatt ttgctgcagc
     1261 tatttgccga cctaacagta ctgtggtttg ttatggatat ggattcggcg atgaacatat
     1321 caatcgcatt ataaaagata tgttaacgat tccctccaca catctggtta taatctcata
     1381 tagtgatcct aacgatagaa taatgaaaac ctatgagaaa ttagggcgaa atgctcaaat
     1441 taccctcctg attggtgatc atctaggtga tctcaagaca ctcgttgagc actatttacc
     1501 aaagcctgca attgatagaa caactatacg aatggcagat cttattaaat caagactggg
     1561 agctcaggag caaacaaccc aggtaaaaac ctctgaaaac gatgaaggag gaacattacc
     1621 atgagccaga caccttttga gcaagccagc agtttggtaa tcggtaccgt tgagtttgta
     1681 tcaccggacg aaataaagat tgccattgat attgaagctc ccgattccat ggctcttaac
     1741 acaggagggc cacgaccatt cccaagggta aatgaatacc tgcttatccc tgttgaacag
     1801 ggttttcttg taggacaggt ggaatggatt acaatagaac gttctccatt tcctaaacgc
     1861 aagggaatgc aggattttgg tctgatagat ttaccatatc ctcttagaag acttagttta
     1921 aatccacttg ggactttacg tccattgcat tctgggaaaa attttgaatt cacccgtgga
     1981 tgcgaagtct tgccatcagt ggggaccccg gcattacttc ctacagacga acagttaaaa
     2041 gcaataattg aatcgggcga gaaaaaacgt gtatgtattg gaacaagccc tgtggcgaac
     2101 aatgcaaaag tatatgttga cccggatcgc ctttttggcc gtcatcttgc tgtacttggc
     2161 aatacaggga gcggtaagtc atgtaccgta gcaggactaa taagatggtg catagaagag
     2221 tcaaaaaaag taaataagga agtcaatgca agatttatta ttctagatcc gaatgatgaa
     2281 tattcagagg catttgataa tttaggaaag gtaaaaaaat ttgctgtaaa acttaaagaa
     2341 gaatgcaatg aaattaatca attaaaagta cccagttgga tgtggaatag ctgggaattt
     2401 gcatcattat cacaagccag tgagaaagca caaaggcctt tgttaaccag ggctttgagg
     2461 gagattaaaa atggatcaga ttttgaaagg gaaactaaat caattcaatt taggagattt
     2521 ttgacaagtc ttaaagttag cttattaaat taccaaaagt caggaccaaa attatctgaa
     2581 tttccgataa aacaaaatta tggaggattc ttagaaggta ctcaaaaatc attaagtact
     2641 tttgacaata ataatatgac taataaagaa gccgaaatta aaaatgaatt aatccaacaa
     2701 atacaacgaa tattagagaa taaaccaaaa gataagggct attttcctcc attctctttg
     2761 caagaagtta atccgttatt aaacattttg tatgaatttg aagctcgggt tggtccttta
     2821 attccttacg aaggacctga tgaggataca cccatcttct ttaaaaatga aaactttctt
     2881 gctcatctta atgaaattgc tcaagaacaa agtcagcaac aatattttga ttattttatt
     2941 atgcgtattc gcagtttatt tactgataaa agaattgcat cagttatatc aactgaaaat
     3001 tgcgatgagg taacattgct acaatggctc aatgattaca ttgggcaaga ttataataaa
     3061 ccttcaatct ccataataga tttatctttg ctcccttctg aagtgctcta tacaattaca
     3121 tcagttatgg ctcgcattgt ttttgaagca catcatcgtt acaagaaact taatgacact
     3181 gtattaccta ctgtattagt agttgaagaa gctcacaatt ttataaaaaa atatgaaact
     3241 tctaatgatg cttcaccaca gcaattatgt acacaaatat ttgagaaaat tgctaaagaa
     3301 ggtcgcaaat ttggacttgg tctggtttta tcctctcaac gtccctccga actttctcca
     3361 acggttttat cgcaatgcaa cacttttttg ctacaccgta tcagtaatga taaggatcag
     3421 gaactggttc acaaattggt accagataac ttgcgtgggc tgttacgtga attgccatcg
     3481 ctaccctcgc aacatgctat tttactagga tgggcttcgg aacttccagt tcttgttaaa
     3541 attaatgatc tgccaaaatt acagcaacca cgctccgacg accctgattt ttggaatgtc
     3601 tggacaggaa gtgatgaggc agataatgct gtagaacgta aaattaattg ggaaactatt
     3661 gcagatgact ggcaacagaa aacatcaagc aatacaaatg aggaaaaaga agaaaatgaa
     3721 aagaatgaat aataatatct taaaattatc atataggata ccattattat gaactcaacc
     3781 accaacgaaa tactgccaga aggctacaag atgacagagc tcgggccact gccggaagaa
     3841 tgggaggtag tgagattggg agaagtggct gattttttcc ttggaagaac ccctaaaagg
     3901 gctgaaaaat cttattggga taatggaaaa tattcttgga ttagtatagc tgatatgaaa
     3961 gattttggct ttatatctaa aactaatgga aaggtatctc aactagcatt tgagaaaata
     4021 ttcaaagaaa atattgttcc gaaaggcaca ctaattatga gctttaaact taccattggg
     4081 agaacagcca ttcttaacat tgatgctctc cataatgaag caataatttc aatttatcct
     4141 aaaggcgaat tattaaaaga atttttattc tattttctgc caacgatcga cttttcaaat
     4201 tattacgaca atgttgttaa aggaaaaact ttgaacagtt ctaaaatcaa aaatctttta
     4261 cttcccctcc ccccacttcc cgagcagcag aagattgccg ctgtgctttc tgcggtgcag
     4321 gaggcaaagg agaaaaccca ggcggtgata gacgccacaa aagtcctcaa aaaatcgatg
     4381 atgaaacacc tgttcacata cggacccgtg cctccagagg aaacagaaaa tgttccgctc
     4441 aaagaaacag aaatcggaaa agtgccagaa gattgggaag tggaaaaact gggagattta
     4501 tcaaccaaga aattaattaa agttcagttt ggctttccat gtggaaaatg gaatgataaa
     4561 ggaataggga ttgctcaagt gcgacctttt aatataaatg aaaatggaaa tattgattta
     4621 acagttttga aatacattga gacagataat gatataggta agtatcttct tcaaaaggaa
     4681 gatatcatat tcaataatac aaatagtgaa gaattagtcg gcaaaactgc attctggaaa
     4741 gatggaaaaa ttaaatatgt tctttcaaat catatgacaa tcattagaat tgttgataaa
     4801 agcaaattaa atgcttattt tctagcaaaa tatcttcata aaagatggaa tgatggtttt
     4861 tataaaacaa tttgtagaag acacgttaat caatcaagca taagtttggc aaggttaaat
     4921 gatatttctc tccctcttcc ccctctccct gttcaacaaa aaattgcttc gattctttcc
     4981 gccattgatg ctaaaatcga agcggaggag aacaagaagc aggcgcttga ggagctgttc
     5041 aaaacgcttt tgcataatct tatgaccgca aaaatcaggg ttaatca
//
LOCUS       DAOSTH010000102         5050 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_15084_length_5050_cov_11.064464, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000102 DAOSTH010000000
VERSION     DAOSTH010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5050
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15084_length_5050_cov_11.064464"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1084)
                     /locus_tag="PLB65_07790"
     CDS             complement(236..1084)
                     /locus_tag="PLB65_07790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78465.1"
                     /translation="MVKKFLGLIICLMSIGYLFSAEKYVTIEVDTVKTYPLTISVAEA
                     RIKTLQEVRQVALERALPQDISLTTFISSFSVEKNNFYDEQVASSVFMNCCSSGRIIE
                     EKILEDYPSFPKKSNVFEYRMKYRATILPLKTIYNSELDLKVQLSETLLKNNEQFTLE
                     LTPNQDGYLYIFDFLPDNSVLVVYPTLHYPESLIIKDTAWKQTLTAVTSPDKKHSIET
                     LYIVFSEVPISGWENFKSNSNFTELIFSAGEESFILFQKWLGRSDPNKRIEKMVQLHI
                     FEQKEN"
     gene            complement(1078..3267)
                     /locus_tag="PLB65_07795"
     CDS             complement(1078..3267)
                     /locus_tag="PLB65_07795"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOQ78466.1"
                     /translation="MLKKICLIVLMSVIICQVIADLNDEVLALKSWIIVPFSSSSLEA
                     ERDAKLAALPDISKGEFENTAQFELRKQDATNRANQIREEYAQKIADARAAYERHQTG
                     LRNRLNYLLAQTRETIIRQGTLESYDADSQKYRVSIPEKSFQIVVPLDKAPQLKNDFG
                     NYQLKVIRQLNDKLEWEYLEAKLSGPAGEFSSTDKAPGISPSSTITTLVPPQLSSKIS
                     FSEPSGNNMLDAEETADLSIEISNTGKGSGYMVQANITITGVNGIDYDKMIYFGELAP
                     GKSQSKSCQISAGQNLSEGTAQLKISFSEQNGFPPEDKIIQFSTKSLIPPDIQITDIA
                     IDDQNKNGKIEQGEQFDLLVRIQNKGNGLAKNVSTKLILGKDIFLLPGSNAENQFDQL
                     SAGEYKDLHYTLVTAKTATNLNLTLDILEAHTQFNKLNQPLNLAFNRQERTAESIIIS
                     GKERKINIENAPALSIDVEQNIPVLSKADNKKWGVIFGIENYRNVSSVPFARRDAEFM
                     KEYFNKVLGIPTENIYFQTDESATLSEFKTVFDPGGWLAKNAGKPGNEIYIYYSGHGA
                     PDPAGNKAYLLPADGNPNYAAISGYGLDQLYANLAQLKVKQITLFLDSCFSGANRDNE
                     TILASARPVFITTTIPSVASNIAVFSAATGNQISSGYSDKQHGLFSYYLMKGLRGEAD
                     SNKDNKLTQKELNDYLSEQVNTQARRMGREQEPQLQSAEPERVILQW"
     gene            complement(3261..3614)
                     /locus_tag="PLB65_07800"
     CDS             complement(3261..3614)
                     /locus_tag="PLB65_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78467.1"
                     /translation="MNEAKKIYDSYNLLKWMIPIIAKYPRKWRYSLGLRIENRRYDNL
                     EPDNRNNNIGLRVLYAIVKFYHTQPELINEKIYRVCKKIAQFCVRILMVSYYFFMVSS
                     SAMSRKPDLFGEKIC"
     gene            complement(3663..4703)
                     /locus_tag="PLB65_07805"
     CDS             complement(3663..4703)
                     /locus_tag="PLB65_07805"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HOQ78468.1"
                     /translation="MRLRLVLFLLAMSAVQLAAQTISNIRISQDKELGYYTITFDLSG
                     KADDLYFIQAVPYKNGNELANPRSFYGKSISERCAARENLTIFWNPVIEGVDKYGWQF
                     KIYAFLAPSTLVFVEGGTFMMGSNEGEADENAVHQVTVSSFYLGNCEVTQKEWTEVMG
                     SNPSCWKGDNLPVEQVSWYDAVDYCNKLSEKEGLKPCYSKSRSKIICDWTANGYRLPT
                     EAEWEYAARGGKQSKGYIYSGSNELGSVAWYYENSGGRTHEVGTKKPNELGIFDMSGN
                     VYEWCWDSYAYYPSDSQSNPKGANERSYIIVRGGSFNSGPDGSCQVYSRAKVSYYADC
                     NFIGFRVLRASF"
BASE COUNT         1401 a         1132 c          897 g         1620 t
ORIGIN      
        1 atgggaacag gtgtaccctt gctgctataa ccaccatcgc taacggcgga aaagacctaa
       61 aactctagac atctaagggt ctatagctct ggaggattgt tagcaatagg tctccatgat
      121 atgagaaagt ggatacaaag agtaaaatat ctaggtgagg gacaatcgtg atagtcctgc
      181 tcttcccgga tcaatttatc ataaaaaatc cgtattgttc gctaaatcct tgttcctaat
      241 tttctttttg ctcaaaaata tgcaactgca ccattttttc tatgcgtttg ttaggatcgc
      301 ttcttcctaa ccatttttga aacaaaataa agctttcctc tccagcacta aatatcaatt
      361 cggtaaaatt actgttactt ttaaaatttt cccacccgga aatcggcact tctgaaaata
      421 caatgtaaag cgtttcaatg ctatgctttt tatctggact tgtaacagct gttagagttt
      481 gtttccaggc agtgtctttt ataattaaag attccggata atgcaaagta ggataaacta
      541 ccaaaacgct gttatccggc aaaaaatcaa aaatatacag atatccatcc tgattaggtg
      601 taagctctaa ggtaaactgt tcgttatttt taagcaatgt ctcgctcaat tgaactttca
      661 ggtccagctc cgaattataa attgtcttta aaggaaggat agttgcccta tacttcattc
      721 tatactcaaa aacattgctt tttttaggga aagaaggata atcctccaga attttctcct
      781 ctataattcg ccccgaagaa caacagttca taaaaacgct ggaagcaact tgctcatcat
      841 aaaaattatt cttttccaca ctgaagctgg aaataaaagt ggtaagcgaa atatcttgcg
      901 gcaatgctct ttctaaagcc acctgacgaa cttcctgcaa tgtttttatt cttgcttcgg
      961 ctacagaaat agtaagagga taggtcttta cagtatccac ttctatggtt acatatttct
     1021 ctgcgctaaa caaatagcca atactcatta aacaaattat cagccccaga aattttttta
     1081 ccattgcaaa ataaccctct ccggttctgc actttgcaat tgtggctcct gttcgcgtcc
     1141 cattctgcgt gcctgagtat ttacctgttc actcagatag tcgttaagtt ctttttgggt
     1201 aagcttgtta tctttattgc tatctgcctc gccccgtaaa cctttcatca aataatagga
     1261 aaataagcca tgttgtttgt cgctatagcc agaagatatc tgatttcctg ttgctgctga
     1321 aaagacagct atattagatg caaccgaagg tatcgtagta gtgatgaaaa ctggtctggc
     1381 acttgctaaa atagtttcat tatcgcggtt tgcacctgaa aaacagctat ccaaaaacaa
     1441 agtaatctgc ttcactttta gctgcgctaa attggcataa agctgatcaa gaccgtatcc
     1501 ggaaatagct gcataattgg gatttccatc cgctggcaat aaataagctt tattccctgc
     1561 tggatcggga gctccatgtc cgctgtaata aatataaatt tcattgcctg gtttgccggc
     1621 atttttagct aaccagccac ccggatcaaa aaccgtctta aattcactca gagttgccga
     1681 ttcatctgtt tgaaagtaaa tattttcagt tgggattcct aataccttat taaaatactc
     1741 tttcataaat tctgcatctc gcctggcaaa aggaacacta ctcacattcc gataattctc
     1801 tatcccaaaa atcactcccc actttttatt atctgcctta gataaaactg ggatattttg
     1861 ttccacatca atagataatg ctggtgcatt ttcaatattt atctttcttt ctttacccga
     1921 tatgataatg gactccgccg ttctttcctg acggttgaaa gccagattta gaggttgatt
     1981 aagcttatta aattgagtat gagcttctaa gatgtccaaa gttaaattta aattcgtagc
     2041 tgtcttggca gttactaaag tataatgtaa atctttatat tctccagctg acaattgatc
     2101 aaactggttt tctgcattgc tgccaggtaa aagaaatata tccttaccta aaattaactt
     2161 ggtacttaca ttctttgcga gtccattacc tttattctga atacgaacca aaagatcaaa
     2221 ttgttctccc tgctctattt tcccattttt gttctggtca tctattgcta tatcagtaat
     2281 ctgaatatca ggaggaatca atgatttagt gctaaactgt ataatcttat cttcaggggg
     2341 aaagccattt tgctcgctga aactgatctt aagctgtgcc gttccctcgc tgaggttctg
     2401 ccctgcgctt atctggcagc ttttactctg gcttttaccc ggggcaagtt ctccaaagta
     2461 gatcatttta tcataatcta tgccattaac tccggtgatc gtgatgtttg cctgcaccat
     2521 ataaccactg cccttacccg tattgctgat ctctatgctt aagtcggctg tttcctccgc
     2581 atccagcata ttattaccac tgggttcgct gaaactgatt ttgctggata gctgaggtgg
     2641 aaccaatgtg gttattgtgc tgcttggtga aatacccggt gctttatctg tggaactgaa
     2701 ttcccctgcg ggaccactta actttgcttc cagatactcc cattccagct tatcgttcaa
     2761 ttgcctgatt acctttaact gatagttgcc gaagtcgttt tttagctgag gagctttatc
     2821 caagggaacc actatctgaa agcttttttc cggaattgat actctatact tttggctatc
     2881 tgcatcatag ctttccaggg ttccctgcct gataatagtt tcccgggttt gtgccaaaag
     2941 gtaatttaac ctgtttctta gcccggtttg gtgtctttca taggcagctc tggcatcggc
     3001 aatcttctga gcgtattcct ctctgatctg atttgccctg tttgtggcat cctgcttgcg
     3061 caattcaaat tgggcggtgt tttcaaattc acctttgcta atatctggca gtgctgccag
     3121 ttttgcatcc cgttctgcct ccagggatga agaggaaaag ggaactatga tccaggactt
     3181 aagtgccaat acttcatcat taagatcagc tataacctga catataatga cagacatcaa
     3241 cacaattaag cagatttttt ttaacatatt ttctccccga ataaatctgg ttttctactc
     3301 attgcagagc tactaaccat aaaaaaataa tacgatacca ttagtatccg aacgcaaaac
     3361 tgggctatct ttttgcacac ccgataaatc ttttcattta tcagttctgg ctgtgtatgg
     3421 taaaacttaa caatagcata tagtacccgc aagccgatat tgttgttcct gttatcgggc
     3481 tctaaattat catacctacg gttctcaata cgcaaaccca aactataacg ccactttcgc
     3541 ggatatttag caattatcgg tatcatccat ttcaaaaggt tatagctatc atagattttc
     3601 ttagcttcgt tcaaaatttt cctcctcccg gggactgcca aataccaaag ctccaaagtc
     3661 cattaaaagc tagccctgag aacccgaaag ccgataaaat tgcaatctgc atagtaactg
     3721 actttggcgc gactgtaaac ctggcaactg ccgtcgggac ctgaattgaa tgaaccacca
     3781 cggactataa tataacttcg ttcatttgct cctttaggat tactttgtga atcacttggg
     3841 tagtatgcat agctatccca gcaccattcg tacacattac cgctcatatc aaatatgccg
     3901 agttcattgg gcttttttgt tcccacttcg tgagtcctgc ctcctgaatt ttcataatac
     3961 caagccacgg aaccaagctc atttgagccg gagtatatat agcccttgct ttgttttcct
     4021 ccccttgctg catattccca ttccgcttcg gtgggcaggc gataaccatt agctgtccag
     4081 tcgcagataa tcttgcttct acttttactg tagcagggtt ttaatccttc cttttcgctt
     4141 agcttattgc agtaatcaac agcgtcatac cagcttacct gctcaaccgg cagattatcg
     4201 cctttccaac aggaagggtt gctgcccatt acttcagtcc attctttttg agttacctca
     4261 caattgccta aataaaaaga ggaaaccgtt acctgatgca ccgcattttc atctgcttcc
     4321 ccctcgttgg aacccatcat aaaagttccg ccttccacaa aaactaaagt agatggagct
     4381 aagaaagcat atattttgaa ttgccaaccg tatttgtcca cgccttctat gacaggattc
     4441 caaaatatcg taagattttc tctcgctgca cagcgttcag agatactttt tccataaaag
     4501 ctgcggggat tggcaagctc attaccattt ttatagggaa cagcttgaat gaagtagaga
     4561 tcatctgcct taccactcaa atcgaaagta atggtatagt agcccagctc cttgtcctgg
     4621 ctgatccgga tgttgcttat tgtctgagca gcgagctgga cagcgctcat tgccaataaa
     4681 aataaaacta accttagcct catagtttgt atcctgctat agtaattttc ataatttact
     4741 ctactaattt gtttaggatt tttgtcaagt ttttttgtgc tctcatcgga aaagaggtag
     4801 tgaagttgct aatgggcgag atggggtaga tatgatacaa catttagatt gacaatccat
     4861 atataataat acctttaggg atggcagata ttaacgacag gtttcaagcc ggcaaaaagc
     4921 atacagaata aagaaagttc cgttcttcta tacatccagc tctcaatccc tctcgtcttc
     4981 tatcctcaaa agcaaacgcc gttagcgatg gcggcgacct actcttccac acagttgccc
     5041 gtgcagtacc
//
LOCUS       DAOSTH010000103         4861 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_15853_length_4861_cov_15.903870, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000103 DAOSTH010000000
VERSION     DAOSTH010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15853_length_4861_cov_15.903870"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..2160)
                     /locus_tag="PLB65_07810"
     CDS             complement(10..2160)
                     /locus_tag="PLB65_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ78469.1"
                     /translation="MQHLRFLSVFFILLGFVSILESTKVSGIVTDVDKKPLESVRLVS
                     GKKTTLTRQNGSFTIEVMDSMQVSRLGYKKQTLSLKEISALPKDNRGIQIILQDEPIQ
                     LPAYYVFAYLGEENISAADVVTLPIDPDKNYNSASELISQTSSFQSTGTQLKGEIAEI
                     NILGNISRHTLVLVDGIAMNTLGEPFDFSRLNSENIASIDVVKNNASVYGGGSAIGGI
                     IMITTKQGAAIKTNLESKTEFGSYGYLMQQITVSGLNPKNSYRLCLNAYNTDNDFPIR
                     KGELIPEDSESIRKYNSKQQLSFSGAYSTRIAKMLTSFSTDYLTFQRELPGPLNFADL
                     YYKSFLEGSSLRPQIRLSANFGNFTWQSNNWLLKDETTYDNTQSLIEGFSQKYKQKMD
                     NYGFRQGLNYNSQFFQAAFSGEYSYNRYRYQDLINPHQSKIDYVRNQFALALKLNQQK
                     EFAWLIVNNGLAGRYDYIEGEDEFSGRLEFSVRNWGNNYLETGATIGNSFALPSPFDL
                     YWKGDSQTLGNPNLKSEKATGYQIWIKGNIPQATLKATLHQNKIKNLIQWRQVQMYGP
                     VWKPMNIGKATVRNLELEGNCQPLKQLELTASAVFTKAKDTTYYSFEDAPKLMYRPEV
                     LYSFALDWQPGIFHFWTKYSFTGKQWITPDNLIDPISAYALMDCGLSLHWQKGNWKFT
                     PALTIKNLTDENYMVHAYVPEPGRTFYATLQLNR"
     gene            complement(2172..2939)
                     /locus_tag="PLB65_07815"
     CDS             complement(2172..2939)
                     /locus_tag="PLB65_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOQ78470.1"
                     /translation="MIELKNVTCGYGDFPVLKDISLQIAEGDFCALLGPNGAGKSTLL
                     YTIMGYLKPSEGYITIFEKDIAKIKHSWLAKQIAFVPQETRSEFDHTVQETVLMGRYP
                     YLGFLQSYSSEDYEAVDAVLENLKLTEFKHRWLSEISGGEKQRVLIARALVQQTPFIL
                     LDESLSQLDINYQIEIMKLLRTIHSKENKTVLIVSHNINLASNYAERLIFLKEGKLLA
                     AGKPEDLMQKETLKDLFAVELDIMINPHTGHPNIIYP"
     gene            3090..3305
                     /locus_tag="PLB65_07820"
     CDS             3090..3305
                     /locus_tag="PLB65_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78471.1"
                     /translation="MIIVLAIYPLNKTDYLLKVKRGILFNLGKEEDLFICTLSKKPGV
                     CLFYFFLSLCKRLSSKKAVDIFSLSGG"
     gene            3340..4188
                     /locus_tag="PLB65_07825"
     CDS             3340..4188
                     /locus_tag="PLB65_07825"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOQ78472.1"
                     /translation="MRENPKSIFTIDVEDYYNVTEERGEPPISEWDFLPSIVESGFMK
                     LLNLLDLYQVKATCFFLGYVAQKYPHLVIEAKKRGHEIASHGMFHRLVYKLSREEFIA
                     DLLSSKKILEDIISEEVISFRSPSFSVTESIPWFFDALVEAGFKIDSSVFPVKRDYGG
                     YQTTQKKPYWIKTDKGMLCEFPISVAPFCGKDFCFFGGGYLRLFPKRIILSMHSKLAR
                     EGNPTLFYIHPREMEPQHPRLKMNKLAYFKSYVNMNTVKPKLEAILSSSKFITCKEYM
                     AIIKDN"
     gene            4189..4833
                     /locus_tag="PLB65_07830"
     CDS             4189..4833
                     /locus_tag="PLB65_07830"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOQ78473.1"
                     /translation="MQNNKKTASFFDGYSGDFNAIYGTGTGAFQRLINKWFRQTMLIR
                     FQKTIDGCKPLEGKTILDIGCGPGIYDVTLAKNSPAYVYGIDFAPGMIELAKNEAKKA
                     GVDKICEFEIADFNTLPEDRKYNYIILMGFMDYMENPKQVIEKAMRLATDKVFFSFPS
                     NKGFLAWQRQIRYKFKCPLYLYSEKQLQELFSNIAPWKYRIENIGRDFFVTLYK"
BASE COUNT         1513 a          890 c          883 g         1575 t
ORIGIN      
        1 ataaaaaagt tatcggttca gttgcaaagt ggcataaaaa gttctacccg gttcagggac
       61 ataagcgtga accatatagt tttcatccgt aaggttttta atagtgaggg cgggggtaaa
      121 tttccagtta cctttttgcc aatgcaagct taatccgcaa tccattaaag cataagcaga
      181 aataggatct atcaaattat cgggagttat ccattgtttt ccggtgaagc tatattttgt
      241 ccaaaagtga aaaatccctg gttgccaatc aagggcaaaa gaataaagaa cttcaggacg
      301 gtacataagt tttggtgcat cttcaaaact ataataagta gtatccttag ctttcgtaaa
      361 aaccgccgaa gcagtaagtt ccaattgttt aagcggttgg caatttcctt ccaattccag
      421 attccgaact gttgcttttc cgatattcat tggtttccaa acaggaccat acatttgcac
      481 ttgccgccat tgaatcagat ttttaatctt gttttggtgt aaggttgctt ttagcgttgc
      541 ctggggaata ttgcctttta tccagatctg ataaccagtt gccttttcac ttttaagatt
      601 tggattaccc aaagtttgag aatccccttt ccaataaaga tcaaaaggcg aaggcagagc
      661 aaaagaattg ccaattgtgg ctcccgtttc cagataatta tttccccaat tgcgaacgga
      721 aaattccagc cgtccgctaa attcatcttc accctctatg taatcgtaac gaccagccaa
      781 accgttattc acaatcagcc aggcaaattc tttttgctga ttaagcttta aggcaagcgc
      841 aaattgattc ctaacataat caatcttgct ttgatgagga tttattaaat cctgatagcg
      901 atagcgatta taagaatatt caccgctaaa agctgcctgg aaaaattggg agttgtagtt
      961 aagaccttgt cgaaaaccgt aattatccat tttttgttta tatttttggg aaaatccctc
     1021 aattaaggac tgcgtattat cgtaagttgt ttcatccttc aagagccagt tattactttg
     1081 ccaagtaaaa ttgccgaaat tggcagagag ccgaatttgc ggacgcagcg aggaaccctc
     1141 caaaaaagac ttataatata aatcggcaaa atttagcggg ccaggaagct ctctctgaaa
     1201 ggttaaatag tcagtagaaa agctagttag cattttggca atacgagttg aatacgcacc
     1261 agaaaaagaa agctgttgtt tactgttata tttccggatg ctttcactat cttctggaat
     1321 caattctcct ttccttatgg gaaaatcgtt atccgtattg taggcattaa gacataatcg
     1381 ataagaattt ttgggattta atccgctaac cgtgatttgt tgcattaaat agccataaga
     1441 gccgaattct gttttgcttt ccagatttgt ttttatggca gcaccctgtt ttgtagtaat
     1501 cattattata ccaccaatgg cagatccacc tccataaaca ctggcattat ttttcactac
     1561 atctatgctt gctatgtttt ctgaattcaa ccgggaaaaa tcaaagggct cgcctaaagt
     1621 attcatcgcg attccatcta ctaaaaccaa agtgtgtctg ctgatatttc ccaggatgtt
     1681 tatttccgct atttcaccct ttagctgagt tccagtactt tgaaaagaag aagtttgtga
     1741 gattaattcc gaagcgctat tataattttt atctggatct attggcaatg ttaccacatc
     1801 tgcagcagaa atattttctt cgccaagata ggcaaaaaca tagtatgcag gtagctgaat
     1861 cggttcatct tgcagaatta tttggattcc tctattatcc ttaggcagag cagatatttc
     1921 ttttagggat agggtttgct ttttatagcc caaacggcta acctgcatag aatccattac
     1981 ttcaattgta aaactgccat tttgtcttgt taaagttgtt tttttcccgc taaccaaacg
     2041 aaccgattcc aaaggttttt tatctacatc tgtaacaata ccagatactt tggtactttc
     2101 cagaatagat acaaaaccaa gaagaataaa gaaaacggat aaaaaacgaa ggtgctgcat
     2161 agcgtaaact cttatggata aataatattg ggatgtccgg tatgcggatt gatcataata
     2221 tccagctcca cagcaaatag atctttcagt gtctcttttt gcatcaagtc ttccggtttg
     2281 ccggcagcca atagttttcc ttcttttaga aatattaacc tttcagcata gttggaagcc
     2341 aggtttatat tgtgagaaac tattaaaacc gtcttatttt ctttactgtg aatggtcctt
     2401 aagagtttca ttatttctat ctgataattt atatccagtt gggacaaact ctcgtccaac
     2461 aaaataaatg gtgtctgctg aactaacgct ctggcaatta ataccctctg tttttcgcct
     2521 ccactgattt cagaaagcca gcggtgttta aattctgtta acttcaaatt ttccaaaaca
     2581 gcatctaccg cttcataatc ctcagagcta taggattgta aaaaacccag atagggataa
     2641 cgacccatca aaacagtttc ctgaactgta tgatcaaact cagacctagt ttcttgaggt
     2701 acaaaggcaa tctgttttgc caaccaagaa tgttttattt tagctatatc cttttcaaat
     2761 atggtaatat atccctcaga tggtttcaaa tagcccataa ttgtgtatag taaagtagat
     2821 tttccagctc cgttaggacc caaaagtgca caaaaatccc cttctgcaat ttgcagagaa
     2881 atatctttca aaaccgggaa atccccgtaa ccgcaagtta cattctttag ctctatcatt
     2941 gttttgtttt ccagtttttt tctccttaag tatgatcatt tttttgcttg cggtttatct
     3001 gtcaactgaa ctttcagcaa agatatgttt gttttttggt aaaaaagtct ttttatatct
     3061 cggcaaccaa ataagaactc ggataaaaga tgattattgt tttagccatt tatccactga
     3121 ataaaacaga ttatttgctt aaagtgaaaa gaggaatatt atttaatttg ggaaaagaag
     3181 aagatttgtt tatatgtaca ctaagtaaaa aacccggtgt ttgtttattt tatttcttct
     3241 tatcgctatg taagcgttta agttctaaga aagcagttga cattttttcc ctttcaggag
     3301 gataggatat tacttgtttt ataggaaatc taaatagtta tgcgagagaa tccgaaatct
     3361 attttcacta ttgatgtgga agattattat aatgttaccg aagaaagagg tgagccacca
     3421 atttctgagt gggatttttt acccagcata gtagaaagcg gttttatgaa attgctaaac
     3481 ttgcttgatc tttatcaagt taaggcaacc tgtttctttt taggatatgt agcccaaaaa
     3541 tatcctcatt tagtgataga agcaaaaaaa agaggtcacg aaattgcatc tcatggaatg
     3601 tttcatcgcc ttgtatataa gctaagcaga gaggaattta ttgctgattt gctatcctcc
     3661 aagaaaatat tagaggatat tatttccgag gaagttatat ctttccggag cccaagtttt
     3721 tcagttacag agtctatccc ctggtttttc gatgctttag tagaagcagg ttttaaaatt
     3781 gattcttccg tttttccggt taaaagagat tatggtggtt atcaaacaac tcaaaaaaaa
     3841 ccgtattgga tcaaaaccga taagggtatg ctttgtgaat ttcctatttc ggtagccccc
     3901 ttttgtggta aggacttttg tttttttggg ggaggatatt tgcgtctgtt cccaaaacgg
     3961 attatacttt ctatgcacag taaactggca agagaaggaa atcctacttt attctatatt
     4021 cacccgcgag agatggaacc acagcatcct cgcttaaaaa tgaacaaact ggcctatttc
     4081 aaaagttatg taaatatgaa tacagtaaag cccaagctgg aagcaatact ttccagcagt
     4141 aaatttatca cttgcaaaga atatatggca ataataaagg ataactaaat gcaaaataac
     4201 aaaaaaacag cttctttctt cgatggctat tctggcgatt ttaatgccat ttacggaact
     4261 ggaactggag cttttcaacg ccttataaat aaatggttca gacaaacaat gctgatccgt
     4321 tttcaaaaga caatagatgg atgtaaaccc ttagaaggaa agacaatttt ggatataggt
     4381 tgtggacccg gaatttatga tgtaactcta gctaaaaact ctcccgctta tgtgtatggc
     4441 atagattttg ctcccggaat gattgaattg gccaaaaacg aagcaaagaa agccggtgta
     4501 gataaaatat gtgaatttga aattgccgat tttaatactc tgcccgaaga tagaaaatat
     4561 aattacatta ttttgatggg ttttatggac tatatggaaa acccaaagca ggttattgaa
     4621 aaagcgatgc gtttggcaac cgataaagtt ttctttagtt ttccttccaa taagggcttt
     4681 ctggcctggc aacgccaaat tcgctataaa tttaaatgcc cgctttatct atatagtgaa
     4741 aaacaactac aagagctttt ttctaatatt gctccctgga aataccggat agaaaatatc
     4801 ggcagagatt ttttcgttac tctgtataag tagcaatagt gtacactatc tccccaaaag
     4861 t
//
LOCUS       DAOSTH010000104         4675 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_16661_length_4675_cov_8.327273, whole genome shotgun sequence.
ACCESSION   DAOSTH010000104 DAOSTH010000000
VERSION     DAOSTH010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4675)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4675
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16661_length_4675_cov_8.327273"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            820..>4675
                     /locus_tag="PLB65_07835"
     CDS             820..>4675
                     /locus_tag="PLB65_07835"
                     /inference="COORDINATES: protein motif:HMM:NF038128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOQ78474.1"
                     /translation="MKKLLLFFATMLFLAFGLYAVDVQIGEGSATSYNLPINSCYGYS
                     YSQQIYLENELNDNGGGPGPITKIRFYLNTSANPITAWDNWTVYLGSTTKDVFLSTSD
                     WVPVSELTEVFSGTIPNPVAGTWLELTLPTPFNYTGGNLVVAVDENSSGYACSASWGV
                     FDCGDNRGMIYYDDDTNPNPANPPQANKGPIATISRIQFDMAAYVPTTPPNCAVLLGP
                     NDNSTVLLDQVLIWKSGGGAPTSYDVYFGDSNPPPYIQNQIETNYMPSTIAGKTYYWK
                     IVPRNAVGAADGCDIWSFLTPTATQLFESFEATTFPPLGWTNPGNFSRNTGITYHGVA
                     SAYKYSTPNESLLTTPMLTITSTSKLEFYARTNAANTFQRIQVKYSTDRLTWEDIGDP
                     VQLGANSPFEYFSVDISSLAGAKSDYYLAFAVYHVGTAGAVFIDKVFGPEVTPVLPGE
                     VTLTVPADGAINVEARAEFSWTPDVTGGIPMGYKVYIDTSNPPAFEWANVTASPFKAE
                     PPLNYSTTYYWRIGAYNAAGETLSSIRSFTTMDDPTITLFQWIEDFGNLASDPFPPLN
                     WKKHSGKLADPTVLYPHPSGTWLQGKWLNNASFENNAAKVRVFSSYINAWLITPPIAI
                     PQDDFELVFDLALMKTNSNNPPQQTGTDDFFAVLVGDGLSWTPANIVRKWDNQGSEYV
                     YNEIPNEGTIIHIPLGEAGTKYLAFYAESTVINADNEIMIDNVKVRQISTCPDPTNLA
                     VSEITSASVILSWTSGGEEELWNVKYGLNGFNPETEGTLVEGVTNPFALTGLSHSTFY
                     DAYVQADCGSNEVSPWSTKVSFRTDCGTHDLPFAEDFNTLSNGDIPYCWTKTHSNWGA
                     VSSTKAGGVANEMRFHYGPSATSVFRLITPPLNGASVSDYLLTFKHYISYYTTPTLFK
                     VQGSTDGENWIDLWSLASPTGNVGPETITIPLTTISGEVFYIAWTFEGNSYNINGWYI
                     DDVTIDLPQECSAPTMLAITNITNSTAKLGWTANSNENKWNIKYGDPGFDPETEGMLI
                     EGVTDNPFIITGLNENSQYDVYVRADCGDGLLSEWSSKVTFQTLCDIYDLPYIQNFDD
                     VTAPAIPMCMTVTDDNGDEKKWVTSTTNPRSSPNCMYMTYNSSISMDDWFFTPGFNLT
                     GGKAYQVDFYYRSSGPNYPEKLKVKWGVEPNAAGMTGGVIWQNDNVINSVYEKATAIF
                     TPNEDGVYYVGWHGYSDPDKFYLCVDDISIYEAPECPPPAMLTATDITTNSALLGWTP
                     GGDETVWNIEYGPAGFELGQGNL"
BASE COUNT         1450 a          780 c          922 g         1523 t
ORIGIN      
        1 attttagtaa attgggacaa aagtaaaata ttttaatcca gattaaacat ttgataaatg
       61 caaaatcatg actagtctta aatatttttt actttgccta aaaggacatt ttatctttta
      121 atttgagtta aatatgcaga aagtgcaaaa tgctaattac ggaaaatttt ttttgtaggt
      181 gtttatcagt agctttttac tttatacttg atagttgtgt aaaaaataaa tttaaaattt
      241 ctttaagtct attttgcttt tttatttaat gagttgttta aaaaagattg tatgttagtt
      301 aggtacatat aaaagcgatg tttaaaagaa aaggttattt ttatttatta taaattacaa
      361 aagataagaa aaataattta cattaaataa aaaattttat atttgcccct tggaaaaatt
      421 aaaggatggg cacatttaag ttttccgttt tacatattct caaactttca cggataccaa
      481 accggtaacg gtttgctgaa atgttgggta tataatggct tacaaatctg taaattaatg
      541 tttcatttta ttaattaact ttttagtcct aacgtttatg aaaagagtta caaaatttat
      601 tcttaatggt tagtattatc ttttgtgata atacttactg atgatctgat aaaagataca
      661 atacgttcag cgattgttac tctaaaaagc aacctaaatt attttgcttt ttgtgacaat
      721 tacaaagtat tataatgtat ctccattatt gatgattatc agctgtttat gaaattaaaa
      781 tcaaaagtta tttttatgtt taactaaaat tcagaaagta tgaaaaagtt attactgttt
      841 tttgcaacaa tgcttttttt ggcatttggt ctgtatgctg ttgatgtaca gatcggggag
      901 ggaagtgcta cttcctataa tttaccaatt aactcttgtt atggctatag ttatagccaa
      961 cagatttatt tggagaatga gcttaatgat aatggtggtg gacctggacc tattacaaaa
     1021 atcaggtttt atttaaatac aagtgccaac cctatcacag cttgggacaa ttggactgtt
     1081 tatcttggga gcacaacaaa agatgtgttt ttaagtacca gtgactgggt gccagttagt
     1141 gagttaacag aggtgttttc tggaacaatt ccaaatcctg ttgctggaac atggcttgaa
     1201 ttaacattac ctacaccatt taattacact gggggtaatc ttgtagttgc cgttgacgag
     1261 aattcttcgg gttatgcctg ttctgcaagt tggggcgttt ttgattgtgg cgataaccgt
     1321 gggatgattt attatgatga tgataccaat ccaaacccgg ctaatcctcc acaagctaat
     1381 aaagggccta tcgctactat ttcaaggatt cagtttgaca tggctgctta tgttccaact
     1441 acaccgccaa actgtgccgt tttacttggg cctaatgaca attcaacagt tttgttggat
     1501 caagtactta tttggaagag tggtggtgga gctcccacat cttacgatgt ctattttgga
     1561 gatagtaatc ctccaccata tattcaaaat cagattgaga ctaattatat gccatctact
     1621 attgctggaa aaacctatta ctggaagata gtaccacgaa atgctgtagg agcggccgac
     1681 ggatgtgata tttggagctt tttaactccg acagcaacgc aactttttga gagttttgaa
     1741 gctacaacat ttcctccact tggctggaca aatccaggaa atttttcacg caacactggc
     1801 ataacttatc atggagtagc ttcagcttac aaatattcaa cacctaacga aagcttactt
     1861 acgacgccaa tgttaactat tacatcaaca agtaagttgg aattctatgc tcgcacaaat
     1921 gctgctaaca cttttcaaag gatacaggtg aaatattcaa ccgatcggtt aacttgggag
     1981 gatattggcg atcccgtaca actaggcgcc aattcacctt ttgagtattt cagtgtggac
     2041 attagttcac ttgctggagc aaaatcagat tattatcttg cttttgcagt ttaccatgtt
     2101 ggaacagccg gtgcggtttt tatagataag gtatttggac cggaagttac tccggtgtta
     2161 cctggtgagg taacacttac ggttccagca gatggagcaa taaatgttga agcaagagct
     2221 gaattctcat ggactcctga tgttactgga ggaataccga tgggttataa agtttatatt
     2281 gatacttcta accctcctgc tttcgaatgg gctaatgtta cggcatcacc tttcaaagcc
     2341 gaaccgccat taaattattc cactacttac tattggagaa taggagctta taatgcagct
     2401 ggtgaaacgt taagttctat aagatcattt actactatgg atgatcccac aataacactt
     2461 ttccagtgga tagaagattt tgggaatctt gccagtgatc cttttccacc tttaaattgg
     2521 aaaaaacatt cagggaaact tgctgaccca acagttttat acccacatcc atcaggtact
     2581 tggctgcaag gtaaatggct taacaacgct tcttttgaaa ataacgcggc aaaagttagg
     2641 gtcttcagct cttatataaa cgcatggctc ataacaccgc caattgcaat tccccaagat
     2701 gattttgaac tggtatttga tctggcttta atgaagacta attcgaataa tcctcctcag
     2761 caaacaggaa cagatgattt ttttgctgtg cttgtgggtg atggattaag ctggacaccg
     2821 gcaaacattg tcaggaaatg ggataatcag gggtctgaat atgtttataa tgaaatacca
     2881 aacgagggaa caattattca tattccatta ggcgaagctg gtacaaaata tctggcattt
     2941 tatgcagaat ccactgtaat taatgctgat aatgaaatta tgattgataa tgttaaagta
     3001 aggcaaattt ccacttgtcc tgatccaacc aatttagcag tttcagagat aaccagcgct
     3061 tcagttatac tatcttggac gtctggcggc gaagaagagc tttggaatgt caaatacgga
     3121 ttaaatggtt ttaatcctga aactgaagga actttagttg aaggtgtaac taaccccttt
     3181 gcacttacag gactaagtca tagtactttt tatgatgctt atgtacaggc cgattgtgga
     3241 agtaatgagg taagcccatg gtcaaccaaa gttagtttta gaactgattg tggtacgcat
     3301 gaccttcctt ttgcagagga ttttaatacc ctgtctaatg gtgatattcc ttactgttgg
     3361 actaaaacac attctaattg gggcgctgtt tcttcaacta aagctggagg tgtagcaaat
     3421 gaaatgaggt ttcattatgg tcctagtgca actagtgtat tcaggcttat tacaccacca
     3481 cttaacggag cctcagtatc tgattatttg cttacattta aacattatat ttcttattat
     3541 acaacaccga cattatttaa agtacaagga tctaccgatg gtgaaaactg gatagattta
     3601 tggtcactag caagtccaac aggtaatgtt ggccctgaaa caattactat acctttgact
     3661 accatttcag gtgaggtatt ttatattgca tggacttttg aaggaaatag ctataacatt
     3721 aatggctggt atattgacga tgttactata gatttaccgc aggaatgtag tgctccaaca
     3781 atgcttgcca ttaccaatat tacaaacagt actgctaaat tggggtggac tgctaatagt
     3841 aacgagaata aatggaatat taagtatgga gatcctggat ttgatcctga aacagaaggt
     3901 atgttaatag aaggtgtaac agacaatcca ttcattatta ccggccttaa cgaaaatagc
     3961 caatacgatg tttacgttcg agccgattgt ggagatggat tattaagtga gtggtcaagc
     4021 aaggtaactt tccagacact atgcgatatt tatgatttac cttatattca aaattttgat
     4081 gatgttacag cgcctgctat tcctatgtgt atgacagtaa ccgatgataa tggagatgag
     4141 aaaaaatggg ttacatctac tactaatcca aggtcttcgc ctaattgtat gtatatgact
     4201 tataatagct ctatttcaat ggatgactgg ttctttacac caggcttcaa tcttacagga
     4261 gggaaagctt atcaggttga tttttattat cgttcgagtg gacctaacta tcctgaaaaa
     4321 ttaaaagtta aatggggtgt tgaacctaat gcagcaggta tgactggtgg tgtgatctgg
     4381 caaaatgata atgtcattaa tagtgtttat gagaaagcaa ctgcaatttt tacacctaat
     4441 gaagatgggg tctattatgt tggttggcat ggttacagtg atccagataa gttttatttg
     4501 tgtgttgatg atatttcaat ctatgaagcg ccagaatgcc ctcctccagc tatgctaact
     4561 gccacggaca ttaccaccaa cagtgcgctt cttggctgga ctcccggagg cgacgagacc
     4621 gtgtggaata tagaatatgg acctgcaggc tttgaattag gtcaagggaa tctca
//
LOCUS       DAOSTH010000105         4601 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_16996_length_4601_cov_12.572151, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000105 DAOSTH010000000
VERSION     DAOSTH010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16996_length_4601_cov_12.572151"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            290..1579
                     /locus_tag="PLB65_07840"
     CDS             290..1579
                     /locus_tag="PLB65_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOQ78475.1"
                     /translation="MNTTGLKNDNIVALVDCNNFYVSCERVFNPKLNNKPVAILSNND
                     GCIVSRSQEIKDLKIPMGAPGFKYEALIKKNGGTLLSSNYALYADMSSRVMEVLAMFS
                     PDIEIYSIDEAFLGFNGFKTRNLAEFGNKIKRTVFQWTGIPVSVGISKTKTLAKVANH
                     FAKRYSAFKGSLCLLNDERIANALAKTPVSEVWGIGRQYDKFLRQNKIETALQLRDAD
                     DKFIDHYLTSTGLKTVLELRGYSCIDLDDAPISKKSIVTSRSFGKQVSELSELQEAVS
                     EYVTRAAEKLRKQNCVAGLLMVFLSTNRFKEGPQYNNSLSTTLFPPTAYTPDLIEKAL
                     NLLEELFLPGFEFKKAGVMLADIIPEKDVPLSFMEVNYLDDKRKKLMETVDKLNRIYG
                     QDTLFYASNGIKKDWKMRRAKLSPHYTTNWNDLPKVK"
     gene            1633..2223
                     /locus_tag="PLB65_07845"
     CDS             1633..2223
                     /locus_tag="PLB65_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HOQ78476.1"
                     /translation="MLYTIENKTQSEIKIKRSRFICTLYPLPHAENVKDLLNEHCRLY
                     ATANHHCYAYIYGFNKDITYYSDAGEPSGTAGKPILNALLRNNLTNVLAVVSRYFGGI
                     KLGVKGLIDAYSGSVEQTILQSKLVEAKQLITISLTCDYPAFEAIKHNVETLSGDISQ
                     IVWAEEVSFLVTLPAEQESAFMENINCLSSNYRLNY"
     gene            2246..3118
                     /locus_tag="PLB65_07850"
     CDS             2246..3118
                     /locus_tag="PLB65_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HOQ78477.1"
                     /translation="MKYLIIILVILMNVTMLNAVEYRIVKATTGETITLQQLAKALQK
                     YDIIFFGEFHDNETLHYLERELVPLLDTKRELIISMEMFERDVQSDLDAYIEGWLSED
                     DFLQKSRPWNNYITDYRPIIEYAKEKKLSVIAANIPRHLAGKMARMGDNFPDTITEED
                     KKYLPDRITSPDDDYKKAFLVTMVDTHSPMMNIDPELLYKSQCLKDDTMAESIVSALK
                     MKPKARVIHFNGDFHSRSFLGTASRIQEMLPKKKVAVISPSSREDWRTGILTKEETQS
                     GTFIILIPPQGGEQ"
     gene            3115..3933
                     /gene="amrB"
                     /locus_tag="PLB65_07855"
     CDS             3115..3933
                     /gene="amrB"
                     /locus_tag="PLB65_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOQ78478.1"
                     /translation="MIRNPMHAGTFYPRFEQQLKRQIETWISKAPNPITSERSLGVIL
                     PHAGYMYSGECATLGMYSISHENIDSFIILHPSHQSNYFDFSVSPYQEYLTPLGTMSL
                     DVELYNRIAPQADQNIPLVLHQEEHSMEIQLPLVNYFFPQAKILPIMIGNQIPAVAKR
                     LAELLYESIYSSTKRIVVLCSTDLSHYHRAHNAEQMDSVLLKDVTSLEPDNLWQDILK
                     GNCEACGIGGILAMLYYGKLCTNAKMRVVQYTHSGIISGNDSQVVGYLSAKLYI"
     gene            3952..4497
                     /gene="amrA"
                     /locus_tag="PLB65_07860"
     CDS             3952..4497
                     /gene="amrA"
                     /locus_tag="PLB65_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOQ78479.1"
                     /translation="MFTNEQKKELLSLARNALKAHFTGETITLPDDPAFQPKRGVFVS
                     LHINNDLRGCIGYIKGYKPIAESVVEMAEAAAFQDPRFPPLTEKELETVIIEISVLGE
                     LIPLLKNELPVIGRDGLYIQNPYGSGILLPQVAVEYNWKPETFLREVCRKAGLPPNAY
                     LEPYSVVFRFTAEVFSEADTF"
BASE COUNT         1478 a          827 c          926 g         1370 t
ORIGIN      
        1 tcaacccatc gttgaggagg agtgaagatt gggtagatga caagttgtat atggttaacc
       61 agcaagaaaa ctaaggtgta ggctaaaaca atctttatgt gctgttaatt ttcctgcaat
      121 tccaccaatt atgattgata aatatatgta gatgataaga aaggataata aaagcggggg
      181 tactttttac ctttattaac caagcacaaa acaacaattc ttgacaatta aaggcaatcg
      241 gaaatagagt aacactaagg aaaattatta atatagagaa agatgagcga tgaatactac
      301 tggcttaaaa aatgataaca tagttgccct ggtggattgc aacaattttt atgtgtcctg
      361 tgaacgggtt ttcaatccta aactcaataa caaaccggta gctattttat ctaataatga
      421 tggttgcatc gtttctcgct cgcaagaaat caaagaccta aaaattccta tgggagcacc
      481 tggtttcaaa tatgaggcgc tcatcaaaaa aaatggcggg acattacttt cttccaacta
      541 tgctctctat gctgatatga gttctcgcgt aatggaggtg cttgctatgt tctctcctga
      601 catagaaatt tacagcatag atgaagcatt tttagggttt aatggtttta aaaccagaaa
      661 tttggcagaa tttggcaata agataaaaag aacggttttc cagtggacgg gcatacctgt
      721 ctcggttgga atttccaaaa ccaaaacgtt ggcaaaagtc gcaaatcatt ttgccaaacg
      781 ctattccgct tttaaaggtt cgctatgttt actaaatgat gaacgcattg ccaatgcatt
      841 ggcaaaaaca cctgtttctg aggtctgggg aattggacgg caatatgata aatttttacg
      901 acagaataag atagaaacag ctctgcaatt gcgagatgcg gatgataaat ttatagacca
      961 ttatttaacc tctactggct taaaaacggt tttggaattg cgtggttatt cttgtatcga
     1021 tttggatgat gcgcctattt ccaaaaaaag tatcgttact tctcgttctt ttggcaaaca
     1081 ggtttccgag ctttcagaat tacaagaagc ggtctctgaa tatgttacaa gggctgcgga
     1141 aaaattgcgt aaacaaaatt gtgtggcagg tctgctgatg gtttttcttt ccacaaaccg
     1201 ctttaaagaa ggtccacaat acaataattc attatccaca accctcttcc ctcctacagc
     1261 ctacactcca gacctgatag aaaaagctct aaacttgctg gaagaacttt tcttgcctgg
     1321 ctttgaattt aaaaaagccg gcgtgatgct ggcagatata attcccgaga aggatgtccc
     1381 cttaagcttt atggaagtga attatctgga tgataaacgc aaaaaactga tggaaaccgt
     1441 tgataaactg aatcggattt atggtcagga cacccttttt tatgccagca acggaataaa
     1501 aaaggactgg aaaatgcgcc gggcaaaact ttctccgcac tatacaacaa attggaatga
     1561 ccttcccaaa gtgaaataaa gtgtgggagt gcataagttc aaagtgcggg agtaaaggaa
     1621 aaggattatc tgatgcttta tactatagaa aataaaacgc aaagcgaaat caaaataaag
     1681 cggtctcgtt ttatttgtac attgtatcct cttccccacg cagagaatgt taaagacctt
     1741 ttaaatgaac attgccgtct ttatgccact gccaatcatc attgctatgc ttacatttat
     1801 ggttttaaca aagatataac ttattattcc gatgcgggag agccatctgg aacagccgga
     1861 aaacctattc tgaatgccct tttgcgtaat aatttaacaa atgtattagc ggttgtttct
     1921 cgttactttg gaggaatcaa attaggagta aaaggtctga ttgatgccta tagtggatcc
     1981 gtggaacaaa ccattttaca aagcaaacta gtagaagcaa agcaattaat aactatttct
     2041 ttaacttgtg attatcctgc ttttgaggca ataaaacaca atgttgagac tctttctggc
     2101 gatattagcc aaattgtttg ggcggaagaa gttagctttt tagttactct ccctgctgaa
     2161 caagaaagtg catttatgga aaatattaat tgcttatcca gtaactatcg cttaaattat
     2221 tagtataatc gttatggaag ggatgatgaa atacctaatt ataattctgg tgattctgat
     2281 gaatgtaaca atgctaaatg cagttgaata tcgtattgtg aaagcaacta ctggagaaac
     2341 tattacctta cagcaattag caaaggcatt acagaaatat gatataatct tttttggaga
     2401 atttcacgat aacgagactt tacattatct tgaaagagaa ttggtgcctt tactggatac
     2461 caaaagagag cttattatct ctatggagat gtttgaacga gatgttcaga gtgatttaga
     2521 tgcctatatt gaaggttggc ttagtgaaga cgatttttta cagaaaagcc gtccctggaa
     2581 taattatata acggactacc gtccaataat tgaatatgca aaagaaaaaa aactaagcgt
     2641 aattgcggct aatatacctc gccatttagc agggaaaatg gcaagaatgg gtgataattt
     2701 tcctgataca ataacagaag aagataaaaa gtacttgcca gacagaataa ccagtcctga
     2761 tgatgactat aaaaaggcat ttctggttac gatggtagat acgcactccc caatgatgaa
     2821 tatcgatccg gaactgcttt ataaatccca atgtttgaaa gatgacacta tggcagagag
     2881 tattgttagt gctttgaaaa tgaagcccaa agcacgagtt attcatttta acggtgattt
     2941 ccacagtagg agctttctgg gaactgcttc cagaattcaa gaaatgctgc ctaagaagaa
     3001 agtcgctgta ataagcccgt ccagtcgtga agattggaga acaggaatct tgacgaaaga
     3061 agagacacaa tcaggaacat ttataattct tattccccca caaggaggtg aacagtgatc
     3121 cgtaatccta tgcacgccgg aactttttat ccccgttttg aacagcaact aaaacgccaa
     3181 attgaaacct ggattagtaa agctcccaat cctattacct cagaacgcag tttgggagta
     3241 attcttcctc atgctggtta tatgtattca ggtgaatgtg cgaccttggg aatgtatagt
     3301 atctcccacg aaaatattga ttcctttata attttacatc ccagccatca atccaattat
     3361 tttgatttca gcgtttctcc ctatcaggaa tatttaactc ctttgggaac tatgtctttg
     3421 gatgtagaac tatataatag aattgccccc caagcagatc aaaatatccc tctggtccta
     3481 caccaagaag aacattctat ggaaattcag ttacctttag taaattactt tttccctcag
     3541 gcaaaaattc tgcctattat gattggtaat caaattcctg ctgtggctaa acgcttggct
     3601 gaattgcttt atgaatcaat ttacagttca actaaaagaa ttgtggtttt gtgttccaca
     3661 gatttgtctc attaccatcg tgctcataac gctgaacaaa tggatagtgt tttgttgaaa
     3721 gatgttactt ctcttgaacc ggataacttg tggcaagata ttcttaaggg caattgtgag
     3781 gcttgtggaa ttggtggaat cctcgctatg ctttattatg gaaagctttg cactaatgct
     3841 aaaatgcgtg tagtccaata tacacattcc gggattattt ccgggaacga ttcgcaggtt
     3901 gttggctatc tttctgccaa attatatatc taagcaaaag caggaggaat aatgtttact
     3961 aatgaacaaa aaaaagaact actatccttg gccagaaatg ctctaaaagc acattttaca
     4021 ggagaaacta ttactctgcc cgatgaccct gcttttcaac ccaaacgcgg tgtttttgta
     4081 agtttacaca taaataatga tctgcgtggc tgtattggct atataaaagg ttataaacct
     4141 attgccgaaa gcgttgtcga gatggcagag gcggccgctt ttcaagaccc ccgttttcct
     4201 ccattaacgg aaaaggaact ggaaacagtg attatagaaa tatctgtttt aggcgaactt
     4261 attcctttgc taaaaaatga actgccagtt atcgggagag atgggctcta cattcaaaac
     4321 ccttatggaa gcggaatctt gctgccccag gtagctgttg aatataattg gaaaccggaa
     4381 acttttttgc gtgaggtttg cagaaaagct ggtttacctc ctaatgctta tcttgaacca
     4441 tacagtgttg tttttcgttt cacagctgaa gtcttcagtg aagcagatac attttgattg
     4501 acagataagc ggtcatattt tttttggaaa ttactgagaa gctaatcgcg ggaatagctc
     4561 agttggcaga gcgcaacctt gccaaggttg atgtcgcggg t
//
LOCUS       DAOSTH010000106         4438 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_17786_length_4438_cov_19.442391, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000106 DAOSTH010000000
VERSION     DAOSTH010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17786_length_4438_cov_19.442391"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..2759)
                     /locus_tag="PLB65_07865"
     CDS             complement(45..2759)
                     /locus_tag="PLB65_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78480.1"
                     /translation="MRRILFFLLLIVLLSSCNLRENLLLPPEISAADYQTGNTIKIFS
                     DYLIKAENDDSYLLLHKESIAEELIHMGDEIVFRKVKTFALRDSLGFQNNAEEQSNTY
                     QFSVLRSGTIISLIASINMAEIYTEVKPSSDPFYLVSFNYYLQASSINPTYYSKKRAH
                     FPVSATGEYALFSIPEEENPTVQHNGRDNFYTVLINKTKAQVAVNFPAAYTSMAGNIT
                     ISLKDKLPEYDKLTAYYPNAAISYPVVDLHTENQLANGLAFLRILNAGKGFFGRQWIY
                     LSENSVYSWSENDPTSGNSNWWIDSNGLYSFLKGSGKYFLLTPLENQNEVTVPLDGSI
                     NSVFLQQVWFDLHSNYLPDTQMKVNIAPDISSIVTDYFQNNPYSFNSTVQAFVINFYQ
                     GSELITALPENNWIEFGFFTNLPENSHNRLFSIYRDKLQDIITYKSSGSSYDANHYSQ
                     VNSYIYSGITSSAAYLYGCLQTAPYQLKVPYYKKRQYLQTENTIISWWNTRKFDYDYL
                     VLNMKPTIPNHPWLKGEPFHISSASALADFCFYTNNEKQSSLPAGFYLAMPVLSPQEN
                     YLLLSTFPYSRLKNYIQGTADCSIQNGWLTIYPEFPGIIINSKINYTNPLQLRTYSTM
                     NFLWNDLIFYTYGNASQTANTIFSIYKTNALADPYSILANQYNLSYTSAVYTISSDSE
                     DNFALFQPVLFFPRTTKGQNLLLYEKREPYYRLYPYTESSAFDPWHFYIDNGFNGIAL
                     ANSGSYASFVDNNPHNSVNISVDNSSQDKVVSLYQAQFVLPKFFINKGVPVGSILNMS
                     KLNNVPGVSNLLSAYQVNISNSSGVTINPDFYNIIGATQEPYVYLPISDVTLLNSAHL
                     FYRNQLGNESELNRVDSFSENYANEYIVVGNCFICTVPNPGIFYITSF"
     gene            complement(2759..3961)
                     /locus_tag="PLB65_07870"
     CDS             complement(2759..3961)
                     /locus_tag="PLB65_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOQ78481.1"
                     /translation="MRYLWLILLLIPGLLFSDDIIPLQFNLITTAYNNMLTGNYELAE
                     KQYQQAAILYPQESSAWEGLLWSLNAQGKFRKTLKTFSDAKTKLPEPEKLYNYYAYAL
                     SMQNRFPEARFYYKQALENMPVNPLANEVSCEGLAYAYLALDNFPEAQTYFSRAAFIS
                     HRPIPAFKPSFNSTVYYKVPGKNKTAYGLMQSAQYKSAKLKLNYEYFQLDNAYFRDLF
                     RADFTYQFTPLEAGINGSYLSGKDERVYPAWQIGAKLCPKLYPGKIALNPELFVSLSH
                     YPRFNVQQISFQPQVYWRDFSFAYALHSVFMDNEPSSTDSVHFAQQLSLSKFLPGDFK
                     LGLYCGFGNDTWMIDNSGVIIDTFNQNGSYYGISLGKELFKHFYLYSYYQKWDSEDLI
                     YFSLAGIY"
     gene            complement(3966..>4438)
                     /locus_tag="PLB65_07875"
     CDS             complement(3966..>4438)
                     /locus_tag="PLB65_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78482.1"
                     /translation="EDLSANDPNDEFYILRIAWLQYLLGRYNDSLKNYQKSNNIFPSL
                     DAQTGILNCQLALGLWNDAHNQAEQILKKFSQNIVVLSKAAYATYMLQNYRATADYYQ
                     RIINISPWDMEARGYLVNNLYLAKDIENARKHYQILKKYAPDSAMAKEYSNIFK"
BASE COUNT         1496 a          718 c          854 g         1370 t
ORIGIN      
        1 tcctaaaatc cttgtttctt aaatctaatc ctaaaatcct tgtttcaaaa actggtgata
       61 taaaatatcc ccggattggg aacagtacaa ataaagcagt tacctacaac tatatattca
      121 tttgcataat tttcgctgaa actatccact ctgtttagtt ctgattcatt tcccagttga
      181 ttgcggtaaa aaagatgtgc cgaatttaag agagtaacat cagaaatagg gagatataca
      241 taaggttctt gtgtagctcc gataatatta taaaaatcag gattaattgt cacaccagaa
      301 gaatttgaaa tattaacctg gtaagcagac aagaggttag agactccagg aacattattc
      361 aatttgctca tattgagaat tgaaccaaca ggtacgccct tattaataaa gaatttaggc
      421 agcacaaact gtgcttgata aagagaaaca actttatctt gggaagagtt atcgacagat
      481 atgtttacgc tattatgagg attgttatct acaaatgagg cataagaacc gctattagct
      541 aaagcgatgc cattaaagcc attatcaata taaaaatgcc aaggatcaaa ggctgagctt
      601 tctgtatatg gatataatcg ataatagggt tctctttttt cgtataaaag cagattctgt
      661 cctttagtgg tgcgtgggaa aaataacact ggctggaaaa gagcaaaatt atcttcggaa
      721 tctgaactaa ttgtgtatac agcagaagta taactgaggt tatattggtt ggcaagaatg
      781 ctatagggat ctgccagtgc attagtttta tatatgctaa agattgtgtt tgctgtttga
      841 gaggcatttc cataagtata aaaaatgaga tcattccaga gaaagttcat cgtggaataa
      901 gttctcagtt gtaacggatt ggtatagttt attttgctat ttataataat ccctggaaat
      961 tcgggataga tcgtcaacca gccattttgg atagagcaat ctgctgttcc ctgtatataa
     1021 ttttttaagc gcgaataggg gaaagtggac aatagaagat agttttcctg tggacttaaa
     1081 acaggcatag caagataaaa acctgcaggc aaagagcttt gcttctcatt atttgtatag
     1141 aaacaaaaat cggccaatgc gctcgcagaa ctaatatgaa acggttctcc tttcagccaa
     1201 ggatgattag gaatagtagg tttcatattt agaacaagat aatcgtaatc aaatttacgc
     1261 gtattccacc aactaattat agtgttttca gtttgcaggt attgtctttt tttgtaataa
     1321 ggaactttta gctgatatgg agctgtttgt aaacagccat aaagatatgc tgcagaagat
     1381 gttatgccag aataaatata agaattaact tggctataat gattggcatc atagcttgat
     1441 cctgagcttt tatatgtgat tatatcttga agcttatctc ggtaaatgct aaataagcga
     1501 ttatgggaat tttcaggtaa attagtgaaa aagccaaatt cgatccagtt attttcggga
     1561 agggcggtta taagttcaga accctgataa aaattgataa caaatgcctg cacagtagaa
     1621 ttgaaagaat agggattatt ttggaagtaa tcggttacta ttgaagaaat atcaggagcg
     1681 atatttactt tcatttgggt atccggtaaa taatttgaat gtaaatcaaa ccagacttgt
     1741 tgtaaaaaaa cgctatttat gcttccatcc aaggggacag ttacttcatt ttgattttct
     1801 agaggagtaa gcagaaaata tttgccgctg ccttttaaga aactatataa accatttgaa
     1861 tctatccacc aattagaatt tcctgaagta ggatcatttt cagaccaact ataaacagag
     1921 ttttctgata gatatatcca ctgtctgcca aaaaagcctt tacctgcatt taggattctt
     1981 aagaaagcaa gaccgttggc taattgattt tccgtgtgta aatccacaac aggataagaa
     2041 atagcagcat tgggatagta ggcagttaat ttatcgtatt caggtaattt atccttcaaa
     2101 ctaatcgtta tatttccagc catcgatgtg taggcagcag gaaaattaac agctacctga
     2161 gctttagttt tattaattaa aacagtatag aaattatctc taccgttatg ttgcacggta
     2221 gggttttctt cttcaggaat agaaaataga gcatattctc cggtagcgga cacaggaaaa
     2281 tgagctcgtt ttttactata ataggtagga ttaatggaac tggcttgcag ataataattg
     2341 aaactaacca gatagaaagg gtctgaactt ggttttactt cagtgtatat ttctgccata
     2401 ttgatagaag caatgaggga aatgatagtt ccggagcgta aaacactaaa ttgataggta
     2461 ttactttgtt cttcagcgtt attttgaaaa cctaaggaat cgcgcagagc aaatgttttc
     2521 actttgcgaa aaacaatttc gtcacccatg tgaatcaatt cttcagctat ggattcttta
     2581 tgcagcaaca aataggaatc gtcattttcg gctttaatta gataatcgga aaagatcttg
     2641 atcgtgtttc ccgtttgata atccgctgca gagatttcgg gaggcaaaag taaattttcc
     2701 cgtaggttac aggaagaaag caaaacaatt aacaacaaga aaaaaaggat acgacgcatt
     2761 aataaatacc tgcaagcgaa aaatagataa ggtcttcgct atcccacttt tgataatagc
     2821 tatataaata gaaatgcttg aaaagttcct tgcctaagga aataccataa tatgaaccat
     2881 tttgattgaa ggtatcgata atcactccgg aattatctat catccaagta tcatttccaa
     2941 aaccacaata taaacccagc ttaaaatcgc ctggcaaaaa tttggaaaga gaaagctgtt
     3001 gagcaaaatg aacactatcc gtggaggatg gttcattatc cataaacaca ctgtgcaaag
     3061 cataggcaaa agaaaaatca cgccagtaaa cttggggctg gaaactaatc tgctgaacat
     3121 taaaacgcgg gtaatgactg agagaaacaa aaagctccgg attcaaagca attttacccg
     3181 gatacaattt tggacataat tttgctccta tttgccaggc aggatagacg cgctcatctt
     3241 ttccggaaag ataactacca tttattcctg cttccaaggg agtaaattgg taagtaaaat
     3301 ctgctctaaa gagatctcgg aaataggcat tatccaattg aaaatattca tagttaagct
     3361 ttaacttagc agatttatat tgtgcagatt gcattagacc ataggcagtt ttgttttttc
     3421 ctggaacttt ataatagaca gtgctattaa aagagggttt aaaagcaggt ataggcctgt
     3481 ggctaatgaa agctgcccgg gaaaaatatg tttgtgcttc aggaaaatta tccaaagcaa
     3541 gataagcgta tgctaaacct tcacacgaaa cttcatttgc caacgggtta acaggcatat
     3601 tttccaaagc ttgtttataa taaaacctgg cttcgggaaa acggttttgc atagaaagag
     3661 cataggcata ataattataa agtttctctg gttcgggcag tttagttttg gcatcagaaa
     3721 atgtctttag ggttttgcga aatttgcctt gtgcatttaa agaccaaagt aacccttccc
     3781 aagcgctact ttcttgtgga tataaaatgg ctgcctgttg atactgtttt tcagctagct
     3841 catagtttcc tgtaagcata ttattatagg cagtagtaat aagattaaac tgcaaaggga
     3901 taatatcatc tgaaaatagc agaccaggga tgaggagaag aatcagccaa agataacgca
     3961 ttccgctatt taaaaatatt agaatattcc tttgccattg cagaatcggg tgcatacttt
     4021 tttaatatct gataatgctt tcgcgcattt tctatatcct tagcaaggta tagattattt
     4081 actaagtaac cacgtgcttc catatcccaa ggtgatatat ttataattct ttgataataa
     4141 tctgctgtgg ctctgtaatt ttgaagcata taggttgcat aagcagcttt actaagaact
     4201 actatattct gtgagaattt tttcaagatt tgctctgctt ggttatgtgc atcattccat
     4261 aaacctaaag ctaattgaca gttcagaatg ccagtttgag catccaaaga aggaaagata
     4321 ttgttagatt tttgataatt tttcagtgaa tcattgtagc gacctaaaag atattgcaac
     4381 caagcaatgc gtaaaatgta aaattcgtca ttgggatcgt ttgcacttag gtcttcca
//
LOCUS       DAOSTH010000107         4397 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_17972_length_4397_cov_15.317135, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000107 DAOSTH010000000
VERSION     DAOSTH010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17972_length_4397_cov_15.317135"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(243..2153)
                     /locus_tag="PLB65_07880"
     CDS             complement(243..2153)
                     /locus_tag="PLB65_07880"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOQ78483.1"
                     /translation="MRRCILLFGIILSFHSYAQNHFNVVHIPDSLRENADAVIRYSNT
                     NYEIKNNVLLKQVSFAITILNENAKERASLVIPYNSYTNIKSVAGVVYNSMGIKTNKL
                     SKDDMADYAAYPSFTLFSDSRIKIARPVIKNYPITFEYIYEIEYSGFLMPESWMPILD
                     YRISLEKGHLQILIPDTIILFRYQNIQPAFNIKKLNGKFLYTWDVDYIKAFEKEPFSK
                     PFSMYMPKIFINIRNTGFYGHKVGYDNWESYGKMIYNLLIDRDKLPRETIEEISALCR
                     NAENDLGKVNLVYSYMQNKVRYVNVTKGIGGYQPIAAADVNKYGYGDCKALANYTRAL
                     LQSVGIKSYYTEIGSGKDKTIAFTDFASIDQTNHAILCVPLTSDTMWLECTNSNMPLG
                     YIGAANSNRKALLVAEEGGKLVNTPTYDEFANRNNVTGQIYIDTTGVVKLELILSYAN
                     ELFDEIMPLMSLSKDEQEKYIIHSMPINGFNLRSYKFENKSTLNYPLGELEIKAEIPK
                     YADLMGKRIAFKPNVINRYNFTLHKKRRTNFYISQSYTQKDSIIIHIPDGYRLEFEFQ
                     PVEFSCQVGSYNMMFTQIANHSFLMKRTLVLKRGEYSIDEYPAIYDFFRKIASYDNKN
                     CVFNKYNRINKN"
     gene            complement(2156..4198)
                     /locus_tag="PLB65_07885"
     CDS             complement(2156..4198)
                     /locus_tag="PLB65_07885"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HOQ78484.1"
                     /translation="MRKQLLLFMVLLSACMVGFPQNSPSKFGKVSMDELANKVCPIDS
                     NAHAYFIFNCGLSHFTYPQTVTREDDATESKGFILETSRHFRIKILDETAFDWANVEI
                     PLYVLGNDKEKIVSFKACTYNLENGKMEKTNFSKGNLIYEKTSDRWETAKAPLPNVKK
                     GSIIEVSYTIVSDFYFNLDKWLFQYSIPVLYSNYEVRIPEYFMYHTNIVGYYPVQVIN
                     SHKYESLTLTYIRGQLNGGDRITNTFNYEVGYNEYESTNIPAFMEDSYLKSSKNYLTA
                     VEFELSATKFPNSPMKSYSNTWEDVINQFLSNSEFGTRVKRTGFLDDVVQQISNKAES
                     QLEKMNMAFRHVANRYKWNEKSSVVTSKSLNNSYREGFGNSADINLCLVGLLQSMDIE
                     AYPMLLSTQSNGFVKLTHPSISNFNYVIALAKIDRKTYLLDATEPLTVVNQLPVRCLN
                     DKGLVVKSGPVEWIDLTLNTNYKSQVTLEYELAEDLQITGRYLSTLEGYAALEQRKEI
                     ISYGSQNDYIQSVSKNIPGIEINQFEIGGMDTLEHPVHLAFDFNYPNQVEEAGDLIIF
                     KPLLFQAIKRSPFKLKERIYPVEYNYHTADLVICQIKIPHGYSIESLPPSINMSLPDK
                     SARYVYTVMENNGKIVITAFFTRNKLTYLPKEYGDLKEFYRMMVDKQNEKIVFKKI"
BASE COUNT         1382 a          795 c          708 g         1512 t
ORIGIN      
        1 cagttttaat taattagtta tcaaaaagtt attaacaatt tgagtttata gacaaactct
       61 atttaaactc ttccttgtaa tttgaattat tattaacttt tttactctat gtgaatgttt
      121 ttatagttca tccctgttaa tttatactta taccgcattt ttaggaattc aattcaattt
      181 tttaaaaatc tcctaagtaa aattcatctt ctaaggtata gaaaattttg agaatgaaga
      241 gattaatttt tatttatcct attatattta ttaaagacgc aatttttatt atcgtaggag
      301 gctatttttc tgaagaaatc atagatagct gggtattcat caatggaata ctcccctctt
      361 ttgagcacta atgttctctt cattaagaaa ctatgattgg caatttgtgt gaacatcata
      421 ttgtaacttc ccacctgaca cgaaaactca accggctgga attcgaattc taatcggtag
      481 ccgtctggaa tatgaatgat gatcgaatct ttttgtgtat aagattgtga aatatagaaa
      541 ttagttcttc gttttttatg caaagtgaaa ttatagcggt tgataacatt aggtttaaaa
      601 gcaattcttt tgcccattaa atcggcatat ttaggaattt cggctttaat ctcgagctct
      661 cctaatggat agtttaatgt acttttattc tcaaacttgt aacttctaag gttaaatcca
      721 ttgatgggca tagaatgaat gatgtacttt tcctgctcat ccttcgacaa gctcatcaat
      781 ggcattattt catcgaaaag ttcattggca taggatagaa ttagttctaa tttcaccact
      841 ccagtggtat caatataaat ttgtccagta acattattcc gatttgcaaa ttcgtcataa
      901 gttggggtgt ttaccagttt tccaccttcc tcggcaacca gcaaagcttt tcgattgcta
      961 ttagcagcac caatatagcc caggggcata ttgctatttg tacattccaa ccacatagta
     1021 tcggaagtta aaggtacaca aaggattgca tgattggttt ggtcaatact ggcaaagtcg
     1081 gtaaatgcaa tggttttgtc tttaccactt ccaatttcgg tataatagga tttaattcca
     1141 acagattgta gtaaagccct tgtatagtta gccaaagctt tgcaatcgcc atagccatat
     1201 ttattgacgt ctgccgctgc aatgggttgg tagcctccaa ttcctttagt aacatttaca
     1261 tatcgtactt tattctgcat ataactgtaa acaagattaa cctttcccaa gtcgttctcg
     1321 gcatttctgc ataaagcact aatctcctct atggtttcac gaggcaactt gtctctatcg
     1381 atcaaaagat tataaatcat tttgccatac gattcccaat tgtcataacc aactttatgt
     1441 ccataaaaac cagtgttcct gatatttata aaaatttttg gcatgtacat cgaaaatggc
     1501 ttcgaaaaag gttctttctc gaatgccttt atatagtcaa catcccaggt atataaaaat
     1561 tttccgttca atttctttat attgaaggct ggttgaatgt tctgatatcg aaacaaaata
     1621 atggtgtcag gaattagaat ctgcaaatgc cctttttcca gcgaaattct gtaatcaaga
     1681 ataggcatcc agctttcggg cattaagaat cctgaatact cgatttcgta aatatactca
     1741 aaagtaattg gataattttt tatcaccggc cttgctattt taattctact atcactaaaa
     1801 agtgtgaatg aagggtaggc tgcataatca gccatatcat ccttagaaag cttgtttgtt
     1861 ttaattccca tcgaattata aaccacgcct gctacactct ttatattcgt ataactgtta
     1921 taaggaatta ctaagcttgc tctttctttt gcattttcat tcaaaatagt gatagcaaac
     1981 gaaacttgtt tcaataagac attgttttta atctcgtagt tggtgttact gtatctaatg
     2041 acagcatcgg cattttccct aagtgaatcg ggtatatgaa ctacattaaa atgattttgc
     2101 gcatagctat gaaaagacaa aataattcca aagagtaaaa tacaacgacg cataattaaa
     2161 tctttttaaa gacgatcttt tcattttgtt tatcgaccat cattctgtaa aattccttca
     2221 aatcgccata ttctttgggt aaatatgtca atttgtttcg tgtaaaaaag gcagtgatca
     2281 caatttttcc attattttcc atcaccgtgt acacgtaacg ggccgattta tccggcaaac
     2341 tcatattaat agacggggga agagattcta tggaataacc atgaggaatt tttatttggc
     2401 atattactaa atctgcagta tgataattat attcgacggg atagattctc tctttgagtt
     2461 tgaaaggaga tcgtttgatg gcttgaaata acaatggttt gaaaatgatt aaatcaccag
     2521 cttcttcaac ctgattagga taattgaagt cgaatgccaa atgaacagga tgctcgagcg
     2581 tatccatacc accgatttcg aattggttga tttcgatccc tggaatattt ttcgaaacgc
     2641 tttgtatata atcgttctgt gaaccatagg atattatctc tttacgctgt tccaaagcag
     2701 catacccttc caaagtgctt aaatatcttc cagttatctg taaatcttct gccaattcat
     2761 actctaaagt aacttggctt ttgtaatttg tatttaaggt aagatcgatc cattctacag
     2821 gaccactttt cactaccaat cccttatcgt ttaggcaacg aacaggcaac tgattaacta
     2881 cagtcagagg ttcagtggca tcaagtaaat aagtctttct atcaattttt gctaaagcaa
     2941 tcacatagtt gaagttggaa atggaaggat gcgttaattt cacaaatcca ttgctttgtg
     3001 tcgacaataa cataggataa gcctctatat ccatactttg taaaagtcca acaaggcaaa
     3061 gattgatgtc ggccgagtta ccaaaacctt ctctataaga attattcaga cttttggaag
     3121 taaccacact gcttttctca ttccatttat aacgattagc aacatgccgg aatgccatat
     3181 tcattttttc gagttgactt tctgctttat tactgatttg ctgtaccaca tcgtctagaa
     3241 atccagtacg ttttactcgc gttccaaact cactgttact taagaattga ttgattacat
     3301 cttcccatgt gttggaataa ctcttcatag gtgaattagg aaattttgta gctgaaagtt
     3361 cgaattccac ggcagtaaga taattttttg aactttttaa ataactatct tccatgaaag
     3421 ccggaatatt cgtgctttca tattcattat accccacttc gtagttgaat gtattggtaa
     3481 ttctatcgcc cccattgagc tggcctctaa tgtaagtaag cgttagagat tcgtatttat
     3541 gactatttat tacttgaaca ggataatagc caactatatt ggtatgatac ataaaatact
     3601 cagggattcg tacttcgtaa ttgctgtaga gtactggaat agaatactga aaaagccact
     3661 tatcgaggtt gaagtaaaaa tcggacacaa tggtatatga aacctcgatt atgcttccct
     3721 tcttcacatt aggcaaaggt gccttggcag tttcccaacg atctgaggtt ttttcgtaaa
     3781 tcagatttcc cttactaaaa tttgtttttt ccatctttcc attttccaaa ttgtaagtgc
     3841 aggctttaaa agaaactatc ttttctttat cattgcccaa aacatagagt ggtatttcca
     3901 cgtttgccca atcgaaagca gtttcatcaa gtatcttgat tctgaaatgc ctcgaggttt
     3961 ccagaataaa tcctttactt tcggtagcat catcctcgcg tgtaacggtt tgaggataag
     4021 taaaatgcga taacccgcag ttgaatataa aataggcatg agcattcgaa tcaatcggac
     4081 acactttatt tgccaattca tccatactaa ctttaccaaa ttttgaaggc gagttttgag
     4141 gaaaaccaac catacaggcg cttaataaga ccataaaaag taacaattgt tttctcatga
     4201 tttttatatc tattgcatta tgtatcaata tgttaatgat gttagaggca atattaaaca
     4261 caataacaac ggtacaaaat taaaaaattt tagattgtca aatagaagaa aaccagaaac
     4321 gatcatataa aaataggaaa cttaatttca gaactcctga tcctataatt gcatatcaat
     4381 attttacaaa agtacca
//
LOCUS       DAOSTH010000108         4282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_18591_length_4282_cov_13.908209, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000108 DAOSTH010000000
VERSION     DAOSTH010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18591_length_4282_cov_13.908209"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     regulatory      145..243
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            310..1086
                     /locus_tag="PLB65_07890"
     CDS             310..1086
                     /locus_tag="PLB65_07890"
                     /EC_number="2.4.2.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR00292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide-dependent adenosine diphosphate thiazole
                     synthase"
                     /protein_id="HOQ78485.1"
                     /translation="MEAQISQAITSGWFTKLQNHLISDVIIVGGGPSGLVCAAELASQ
                     NIKTTLIEKDLAPGGGMWGGAMFFNQIVFHKDALQLLERFAISYSYYGNDLYVCDAVE
                     ATSSLIYNATHKGAGIFTGICMEDVLVKDNRVSGVVINWSTVKKDRLHIDPLSLSAQA
                     ILDATGHPCEVVSVLCRKNTIHLNLPSDTIPYEKSLNAEAGEKSCLEFTQEVYPGLFV
                     SGMAACGVSGSNRMGPIFTSMLLSGMKAADLILQSLNREN"
     gene            1089..1742
                     /locus_tag="PLB65_07895"
     CDS             1089..1742
                     /locus_tag="PLB65_07895"
                     /inference="COORDINATES: protein motif:HMM:NF014622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOQ78486.1"
                     /translation="MDNFGLYIVMTNPLLGYETFTEICVQEEVPMLQIRDKHLSDKEL
                     LNLCKRLKAITEGSKTRFIVDDRPDICLLTKADGLHLGQDDLPWKEVLPFLPTNTLLG
                     VSTHNLKQAKELMESITAQKNLQKPDYFSFGPIYPTVAKEKPDPAIGVEDLKTVLNLS
                     PIPVVAIGGLFPENLPNVLAVGAQNMAFIRHFTLSRETAELKAKIREMKSLLKEAQK"
     gene            1739..3010
                     /gene="thiC"
                     /locus_tag="PLB65_07900"
     CDS             1739..3010
                     /gene="thiC"
                     /locus_tag="PLB65_07900"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: protein motif:HMM:NF014067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HOQ78487.1"
                     /translation="MTQLQQATKGIATSEILTAAKEEHIEADILMQLIKEGKVVIPHN
                     QNRKAIPTAIGSKMTTKINANIGTSELCCEPDTEIAKLQLCKKYQAHSVMDLSTGGNL
                     DNIRIKMLEATDLILGTVPLYAVATELQRTDKDITAFEPEMLFAEIEKQAEQGVDFMT
                     VHAGINQWSLKAHQKENRLTGIVSRGGAFIKRWMLHTGKENPLYEQFDRLLNICLKHD
                     VTLSLGDGFRPGAICDATDCTQISELLVLSELVKRARLKGVQVMVEGPGHIPLPEIEA
                     NVLLEKKLCAEAPFYVLGPLPTDFACGYDHITGAIGGAIAASFGADFLCYVTPSEHLC
                     LPNLEDVKQGIIASRLAAHIADIAKGNANAREIDNIIAKARREMDWETIFRYSIDPEL
                     ARKRKSETSSEVQDQCSMCGKLCAIKTDKQS"
     gene            3014..3781
                     /locus_tag="PLB65_07905"
     CDS             3014..3781
                     /locus_tag="PLB65_07905"
                     /inference="COORDINATES: protein motif:HMM:NF020132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOQ78488.1"
                     /translation="MQLRTNLAFIEPRDPTFHTGLELDYIMTLGLGFAPVFCSSGLTI
                     QSEKGVEEIIPVNNQNFEAGIRKIVLSDKVQYCKIGALCSVEQVNLLEQTLSSKRELK
                     IVVDPVFTPTKGKAFLTESALPDFLKLMNLVDFITPNLPELSFLTGKDTTAFETAIEV
                     GKELSQKTGVTVLLTGGHGKTNNIQEAMINKKEVTIFHFPRSKWIYSHGTGCVFSTFF
                     TCSLAMGNDPVEAFTEASNFVRKLFDAYNAKISPENQ"
BASE COUNT         1286 a          850 c          885 g         1261 t
ORIGIN      
        1 ctaaaatcct tgttcctaaa actcaaatct aaaatcccac agaatccttt tcgcccaaag
       61 ctctttgccc cctgcaatca taatcctgtt catcccctaa ttattttatc ttttgcttga
      121 catttaggtg ccaagtattt ttgtaaccta aaacaggggt gctggaaaat attccggctg
      181 agattatacc cttcgaacct gatccggata ataccggcgc gaggaagaag accttaaatt
      241 tcactcagtt aaccatcgaa ggataaagct catacaaaca accccttaaa aaactaaaaa
      301 ggggacaaaa tggaagctca aatttcccaa gccattacca gcggctggtt tacaaagctg
      361 caaaatcatc tcatcagtga tgttataatt gttggaggag gtccttcggg tcttgtttgt
      421 gccgcggaac ttgcctcgca aaacatcaaa accaccctta ttgaaaaaga ccttgctcct
      481 ggaggcggga tgtggggtgg agcgatgttt tttaatcaaa tcgttttcca taaagatgcg
      541 cttcagctat tagagcgttt tgcaatttcc tatagctatt atggcaacga cttatatgtt
      601 tgcgatgccg tggaagcaac ttcctctcta atttacaatg ccactcataa aggagcgggc
      661 atttttaccg gtatttgtat ggaagatgta ctggtaaaag ataatcgggt ttcaggtgta
      721 gtgataaact ggtcaactgt aaagaaagac agattacata ttgatccctt atctttatcc
      781 gcccaagcca ttttggatgc aactgggcat ccttgtgagg tagtgagtgt tttatgccgc
      841 aaaaacacta tccacttgaa tttgccctct gataccattc cttatgaaaa gtctttaaat
      901 gctgaagcag gcgaaaaatc ctgtttggaa tttacgcaag aggtctatcc gggacttttt
      961 gtttccggta tggctgcttg tggagttagc ggcagtaata gaatgggtcc tatttttacc
     1021 agtatgttgc tttccggaat gaaagccgcg gatttgattc tgcaatcgct aaaccgggag
     1081 aattaacaat ggataacttc ggcttatata tcgttatgac taatccgctt ttgggttatg
     1141 agacattcac cgagatctgt gttcaggaag aagtaccgat gctgcagatt cgcgataaac
     1201 atctttccga taaagagttg ctgaatcttt gcaaaaggtt gaaagctatc accgagggct
     1261 ctaaaaccag gttcatagtt gatgaccgac cggatatctg tcttttgaca aaagcagatg
     1321 gcttacattt agggcaggat gatcttccct ggaaagaggt tttacctttt ttgcccacca
     1381 acactcttct aggtgtttct acgcataatt taaagcaggc aaaagaatta atggaaagta
     1441 tcactgccca aaaaaaccta caaaaacctg attatttcag ttttggcccc atctatccta
     1501 ctgtagccaa agaaaaacct gatcccgcta taggggttga ggatttaaaa actgttctga
     1561 atttatctcc tataccggta gtggcgattg gagggttatt tcctgaaaat ctgcctaatg
     1621 ttttagccgt cggagcccaa aatatggcat ttatcaggca ttttactctt tcccgcgaaa
     1681 cagcggaatt aaaagccaaa attagagaaa tgaaatcatt attaaaggag gcacaaaaat
     1741 gacccaatta caacaagcaa ccaaaggcat agccacttcc gaaatattaa ccgcagccaa
     1801 agaggaacat attgaagctg atattcttat gcagcttata aaagaaggca aagtggttat
     1861 ccctcataac caaaaccgta aagcgatacc aactgctatc ggaagtaaaa tgactaccaa
     1921 aataaatgcc aatatcggaa cttcggaact ttgctgtgag ccagacaccg aaattgccaa
     1981 gctgcaactg tgtaaaaaat atcaagctca tagcgttatg gacctttcca caggtggcaa
     2041 tttagataat atccgcataa aaatgttgga agcaacagac ctaattttgg gaacggtacc
     2101 tctttatgct gtagctacag aattacaaag aaccgataaa gatatcactg cttttgaacc
     2161 cgaaatgctc tttgccgaaa tagaaaaaca ggcagaacag ggagtggatt ttatgaccgt
     2221 gcacgcaggt attaaccaat ggtcacttaa ggcacatcaa aaagaaaatc ggttaacggg
     2281 aattgtaagc agaggaggtg cttttatcaa acgctggatg ctacatacag gaaaagaaaa
     2341 tccgctttat gaacagttcg accgcctgct aaatatttgc ctgaaacacg atgtaacact
     2401 aagtttggga gatggcttca gacctggagc aatttgtgat gccaccgatt gtacccagat
     2461 ttcagaattg cttgtgctga gcgaactggt taaaagagcc cgcctaaaag gagtgcaagt
     2521 tatggtggaa ggtccggggc atatacctct tccagaaatt gaagctaatg ttttgctgga
     2581 gaaaaaatta tgcgctgaag ctccttttta tgtgttgggt cctctgccaa ctgattttgc
     2641 ttgtggttat gatcatatca caggagccat tggaggtgcc atagcagctt cctttggagc
     2701 cgatttttta tgctatgtaa ccccctccga gcatctttgt ctgccaaatc tggaagatgt
     2761 aaaacagggc attatcgctt ctcgcctggc agcccatatt gcagatattg ccaaaggaaa
     2821 tgctaacgcc agagaaattg ataatattat cgccaaagcc agaagagaaa tggattggga
     2881 aaccatattc cgttatagta ttgatcctga gttggcaaga aaacgcaaaa gcgaaacttc
     2941 cagcgaggtt caagatcaat gcagtatgtg cggaaaacta tgtgctataa aaacagataa
     3001 gcagtcataa ataatgcaat tacgaacaaa tctggcattt attgaaccca gagatcctac
     3061 cttccatacg ggtttggaac tggactatat aatgacttta ggtttggggt ttgctcctgt
     3121 tttttgttcc agcggattaa ccattcaaag cgaaaaagga gtggaagaaa tcattcccgt
     3181 aaataaccag aactttgaag ccggtattcg aaaaatagtg ctttctgata aagttcaata
     3241 ttgcaaaata ggtgcgttat gtagcgttga acaagttaac ctgctggaac aaactctttc
     3301 ctcaaaacgg gaacttaaaa ttgttgttga tcctgttttt actccgacca aagggaaggc
     3361 atttttaact gaatcagccc tgcctgattt tttgaagcta atgaatctgg ttgattttat
     3421 aactcctaat ttgcctgaac tatctttttt aaccggaaaa gatacaactg cttttgagac
     3481 ggctattgag gttggaaaag aacttagcca aaaaacggga gtaactgttt tgctaactgg
     3541 agggcacggc aaaacaaata atatccaaga agctatgatt aataaaaagg aagtaactat
     3601 tttccatttc ccgcgtagta aatggatata ttctcacgga acgggttgcg tgttttccac
     3661 cttttttact tgttctttgg caatgggaaa tgatccggtt gaggcattta ctgaggcatc
     3721 taactttgtg cgtaaacttt ttgatgccta taatgcaaaa atctctccgg aaaatcagtg
     3781 aataggagtt caaagtgcgt agttcaaagt gcgtagttca aagttatttc ttccgtggct
     3841 tttgtaactt cggtgctttc ggtggctaaa tattcttcta aaatccttgt ttctaaaact
     3901 caaatctgtc gtcccgctgg gactttttat atttgtactt tttgtcgccg ggttaaaacc
     3961 cgtcgttagt atgtatcgtt cctagcggaa cttttttcat tgtatgtctt ccttttcgag
     4021 gggcttacgc caccgtcgct atgatcgtgt cgccctaagg ggctttattc acatcttata
     4081 taataagcag ttatataata taatcctctt aatcctataa tccttaaatc cttgtttcta
     4141 aagctatatc tgtgtaatcc gtgtaatctg tgagaggata tttttttcgg tggcttcggt
     4201 gctttcggtg gctaaatatt cttctaaaat ccttgtttct aaaactcaaa tctgtcgtcc
     4261 cgctgggact ttttatattt gt
//
LOCUS       DAOSTH010000109         4226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_18906_length_4226_cov_15.274754, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000109 DAOSTH010000000
VERSION     DAOSTH010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18906_length_4226_cov_15.274754"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..889
                     /locus_tag="PLB65_07910"
     CDS             23..889
                     /locus_tag="PLB65_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78489.1"
                     /translation="MKTRYLLILFTLIVLFGAWLYLSYQIGISETDKLEKLANLPIYA
                     YVADTTQVAAILSELKPVPGIKNVVYETPMQAASELIQAYALPITEEMISDYALPAII
                     TINLEPNRESISSKPIIMDILRSHLPEADIDSQSSAYRIIIEELKHLDLYNIVFNVVI
                     GILILIIYIFSRTTLELHKLFHYKGNKHSAIENIRLQRQSVKHTWLMLLVPLPICLIG
                     YFAWIYFKPLPQLIPYWVFLVQAAAAILGTLINHFILHSFERQMVMEENPIEVIYQKN
                     NGTEDNNDTPLT"
     gene            870..1427
                     /gene="pgsA"
                     /locus_tag="PLB65_07915"
     CDS             870..1427
                     /gene="pgsA"
                     /locus_tag="PLB65_07915"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOQ78490.1"
                     /translation="MIRHLPNLLTIFRILLVPIFLYTVFLTQYQSRILVALVIFIVAS
                     LTDYLDGKIARKLNLISDFGKIMDPLADKLIVLSALAALCWLPPFQVSIIVFFVIFLR
                     ELVITIMREVYQKKGIVVAADIFGKLKTFLQMAGIIIAFAFWAFCDPVSPVVILIVNI
                     WFWFVALITVLSGLNYLKATKKERS"
     gene            1430..2491
                     /locus_tag="PLB65_07920"
     CDS             1430..2491
                     /locus_tag="PLB65_07920"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HOQ78491.1"
                     /translation="MRKYILFIPLLFVLFACTQEKGKIDRYTIYDKVIDHSKPLAMGD
                     DRDVYVFCDSINWTGLKPFIGSAIEQQINIVYPEKYFNLIMADIKDAQKLSKYKNLLY
                     IGHLDSDLPVSTFVQKSLSKDFIKRVQNSGGDLFAAENANCCDQIALFLLGKDAKTLQ
                     KVGALQAENIFALLLKRFTQRQGYYAYQGKVIDPSFFDKYPFSLKIPDTYRLYSNDSA
                     GRFLSFIYRARMENREVPDKYISIYYEPMPENKVDLAWLISKRQEIGQKYFEGDEFDP
                     ELVRKEPFKFKKRDGYRLLGAWKNMKYAIGGGFQSYGFWDEKTKTAYIVDSSVFFPAG
                     DKLPVLLELYVISNSLEIK"
     gene            2488..>4226
                     /locus_tag="PLB65_07925"
     CDS             2488..>4226
                     /locus_tag="PLB65_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78492.1"
                     /translation="MNNFLPTKKLLSWLNIILLFFLAACAGPQKQPQRNIIDNSKPNI
                     PSLYYYISASLQYYAGEFLVADDYYKLALDQDYGSYQIRKQILINSAFAFFNNQQDAQ
                     TTVSLYDKARKEIDFDSDLLNVAYSIYSYTNNEEGLAWVIAESVLYYPSTINYLRKFY
                     LEYSKNKKADIQYLELAYKYADHNPEDLILTAKMYSLVNSKRSAAILKEAYILEPKPE
                     TDNLLNEIILKYLGKEEAQNKFLAYQYPIDKEQMLSFLLMANQNRQFEIVNSLNNQIF
                     KTGDLALLSELALSAYLADETDILQELSRFLLQINPEPNINSQVAIILFAEALFSDNL
                     QEPIIYTDYFCSASDLQDAIIFAMLKYSMKTKSKEINSEFSADLVLSVQKRLPESVFA
                     DYLKIAARANTVEDQDFLQARANLCNYFLQKNLGELDDWTFLLQYYHQQNRQEEKITL
                     LRKAINIYPDNPLFLNDLGYTLLDFSEHLNEAGLLIQRAVALDPANAYYQDSLAWYYY
                     LTSDFNSALEHINLPMQLKDMPAEISYHIGMILIANQKNEEAIKYLQAVTEDQTNPLY
                     QEKARQALEELQK"
BASE COUNT         1321 a          756 c          766 g         1383 t
ORIGIN      
        1 aaataaccaa aaggattaag caatgaaaac ccgctatcta cttatcctgt ttactcttat
       61 cgtccttttt ggtgcttggc tatatctgag ctaccaaata gggataagcg aaacagataa
      121 attggaaaaa ctggcaaact tgccaattta tgcttatgtg gctgatacta cacaagttgc
      181 tgcaatatta tcggaattga aacctgttcc cggaattaaa aatgtggttt atgaaacacc
      241 tatgcaagct gcttctgaac ttattcaagc atacgcttta ccaattactg aagaaatgat
      301 cagtgattat gctttgccag ctataattac gattaacttg gagcccaatc gtgaatctat
      361 tagcagtaaa ccaattataa tggacatatt gcgttctcat cttccagaag cagatattga
      421 tagtcaatcc agtgcttatc gaattattat tgaagaactt aagcacctgg atttgtataa
      481 catcgttttt aatgttgtta tcgggatctt gattctgatt atttatatct tcagtcgtac
      541 tactctggaa ttgcataagt tgtttcacta taaaggtaat aaacatagtg cgattgaaaa
      601 cattcgcttg caaagacaaa gtgtgaaaca tacttggtta atgcttttgg tgccattacc
      661 catttgtctg attggctatt ttgcctggat ttattttaaa cccttgcctc agctgattcc
      721 ttattgggtg tttttagttc aggctgctgc tgctattttg ggaactttga tcaatcattt
      781 tattttgcat agtttcgagc gacagatggt tatggaagaa aatcctattg aagttatcta
      841 tcaaaaaaac aacggaacgg aagataataa tgatacgcca cttacctaat cttttaacaa
      901 tcttccggat tttgttagtg ccgatttttc tttatacggt gtttttaacg caataccaat
      961 caaggatatt agttgcccta gttattttta ttgtagccag cttaaccgat tacctggatg
     1021 gaaagattgc ccgtaaacta aatttgatta gcgattttgg taaaattatg gaccctttgg
     1081 cagataaact gattgtccta tctgctttag ctgctttatg ttggcttcct ccatttcagg
     1141 ttagtattat cgttttcttt gttatttttt tacgcgaact ggtgattact attatgcgcg
     1201 aagtctatca aaaaaaagga atagtagtag ctgccgatat tttcggtaaa ttaaaaacat
     1261 ttttacagat ggccggaata attatcgctt ttgcgttttg ggctttttgt gatcctgtct
     1321 caccggtagt tattcttatt gttaatattt ggttttggtt tgtggcactt attacggtgc
     1381 tgagtggttt gaactattta aaagctacaa agaaagaaag gagctaacta tgcgtaagta
     1441 tatacttttc atcccgctat tattcgttct ttttgcttgc acccaagaaa agggtaaaat
     1501 agaccgctat actatttatg ataaagtaat tgatcattcc aaacctttgg ctatgggcga
     1561 tgatagagat gtttatgtat tttgcgattc gataaattgg acaggactta aaccctttat
     1621 tggcagcgcg atagagcaac aaatcaatat tgtttatccg gaaaagtact ttaacctgat
     1681 tatggcagat atcaaggatg cccaaaagct ctccaaatat aagaacctgt tatatattgg
     1741 gcatctggat agcgatctgc ctgtttctac ctttgtgcaa aaatctctta gtaaagattt
     1801 tataaaacga gtgcaaaata gcggaggaga tctttttgca gctgaaaatg ccaattgctg
     1861 cgatcaaatt gcccttttcc tcttaggaaa ggatgctaaa actttacaaa aagtaggcgc
     1921 cttacaagcc gaaaatattt ttgctctgct cttgaaacgc ttcacccaaa gacagggtta
     1981 ctatgcctat cagggaaaag tgattgatcc ctcctttttt gataaatatc ctttttccct
     2041 taaaattccc gatacctatc gtttgtattc caatgactct gctggcagat ttctaagctt
     2101 tatatataga gctagaatgg aaaatcgaga agttccagat aaatatatat ctatttacta
     2161 tgaacctatg cccgaaaata aagttgacct tgcttggttg atttccaaac gccaagagat
     2221 tgggcagaaa tatttcgaag gcgatgaatt cgatccggaa cttgttcgca aggaaccatt
     2281 caagtttaaa aaacgcgatg gttaccgtct tctgggtgcc tggaaaaata tgaaatacgc
     2341 tatcggaggc ggttttcaaa gctatggttt ttgggatgaa aaaaccaaaa ctgcatatat
     2401 agttgatagc agcgtcttct ttccagctgg agataagctt cctgttttat tggaactata
     2461 tgtgatctcc aattcactgg aaattaaatg aataacttct tacccaccaa gaagttacta
     2521 tcttggctaa atattattct attgtttttt ttggctgcct gtgctggacc ccaaaaacaa
     2581 cctcaaagaa atattataga taattccaag cccaatatcc cttccctgta ttattatatt
     2641 tctgcttctt tgcaatatta tgcaggagaa tttcttgttg cagatgacta ctataaactg
     2701 gctctggatc aagattatgg cagctatcaa attaggaaac aaattctaat taatagtgcg
     2761 tttgcgtttt ttaataatca gcaggatgca caaactactg taagtctata tgataaagcc
     2821 aggaaggaaa tcgattttga cagcgacctg cttaatgttg cctattctat ttatagctat
     2881 accaataacg aagaagggct tgcttgggtt attgctgaaa gtgttttata ttatccttct
     2941 acgattaatt atctacgcaa attttatctg gaatatagca aaaataaaaa agctgatatt
     3001 caatatttag agcttgccta taaatatgcg gatcataatc ctgaggacct gattcttact
     3061 gccaaaatgt attcattagt taattctaaa cgctcggcag ctattttgaa agaagcttat
     3121 atactagagc caaaaccgga aacagataat ttgctaaatg aaattatcct taagtatttg
     3181 ggtaaagagg aagcgcaaaa taaattcctg gcttatcagt atcccataga taaagagcag
     3241 atgctatctt ttttactaat ggcaaatcaa aaccggcaat tcgaaattgt taactcccta
     3301 aataatcaga tatttaaaac cggcgatctg gctcttttaa gtgagctggc tttatctgct
     3361 tatcttgcag atgaaacaga tattttacag gagttatcca gattcctgtt gcaaataaat
     3421 cctgaaccaa atatcaattc acaggtagct attattctct ttgccgaagc tctgttctct
     3481 gataatctac aggaaccaat catttataca gattatttct gttcagcttc agacctgcag
     3541 gatgctatca tattcgcaat gctgaaatat tctatgaaaa ctaaatctaa agaaataaat
     3601 tctgaattct ccgcagattt ggttctatct gtgcaaaagc gtttaccgga aagtgttttt
     3661 gccgattatc ttaagattgc agcaagagct aacactgtgg aagatcagga ttttctacag
     3721 gcaagagcaa atctatgtaa ttactttcta cagaagaatc tgggtgaatt ggacgactgg
     3781 acttttctcc tgcaatatta tcatcagcag aataggcagg aagagaaaat cacccttctg
     3841 cgaaaagcta taaatatata ccccgataat cctctctttc taaacgactt gggttatact
     3901 ttgctggatt tttcggaaca cttaaatgaa gccggtttgc tgattcaaag agcagtagct
     3961 ttggacccgg caaatgctta ttatcaagat agcttggctt ggtattatta tctaacttcc
     4021 gattttaata gtgctttgga acatattaat ttgccaatgc aattaaaaga tatgccagca
     4081 gaaatctctt accatatcgg gatgattctt attgccaatc aaaaaaacga agaagcaata
     4141 aaatatctgc aagccgtaac ggaagatcaa accaaccctc tctatcagga aaaagccaga
     4201 caggcattag aagaactgca aaaata
//
LOCUS       DAOSTH010000110         4211 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_19005_length_4211_cov_6.915784, whole genome shotgun sequence.
ACCESSION   DAOSTH010000110 DAOSTH010000000
VERSION     DAOSTH010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19005_length_4211_cov_6.915784"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..827)
                     /locus_tag="PLB65_07930"
     CDS             complement(90..827)
                     /locus_tag="PLB65_07930"
                     /inference="COORDINATES: protein motif:HMM:NF015914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3944 domain-containing protein"
                     /protein_id="HOQ78493.1"
                     /translation="MGNKYKQDSDLEFLQFCDNEDLSILVDFLTKDKKGNDRITSDLL
                     SDDRFNNCKGNYKRAWDLIATELQYYGGDTILNIIRRHGISYREILTDVCKKLKVNFN
                     KKSSVEKIEANLFLKIIEDSLEKMTDEQKREFAKGMKLNLKHASSVAIMAALQSAIIA
                     GEFTSYKIALYVANSVAKAILSRGLSFGVNAGLARGLAIFAGPVGWVITSILTIPMFS
                     GTAYRVTIPSVIQIAYMRQKYLNKEAL"
     gene            complement(1348..1593)
                     /locus_tag="PLB65_07935"
     CDS             complement(1348..1593)
                     /locus_tag="PLB65_07935"
                     /inference="COORDINATES: protein motif:HMM:NF018500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HOQ78494.1"
                     /translation="MSIHIIPVNKLSAHALKGVIEEFISRNGTDYGAIEASLETMVRQ
                     VKSKLKDGSAVLVFDDETETTNIFLADDPILKKLEGV"
     gene            complement(1607..1927)
                     /locus_tag="PLB65_07940"
     CDS             complement(1607..1927)
                     /locus_tag="PLB65_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78495.1"
                     /translation="MATGSRVGYYAYMTVEGSDSMTPIESIYDIEAAALYLQKYLRPK
                     NRVVPKRAQMIYEQLVDRFFREHVNLVEQGKRRSLRLDCLQDVNSFLRLLETAKEQYS
                     FYDD"
     gene            2142..2417
                     /locus_tag="PLB65_07945"
     CDS             2142..2417
                     /locus_tag="PLB65_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78496.1"
                     /translation="MKKTLGTIVAAAAVVLLTATFGFAEYAATGAANFPYFQLGCLII
                     GGLLMVALKKKYEKMYTAEVVGAFALYTILMALFTNPVIDMVKIIVT"
     gene            complement(2678..3856)
                     /locus_tag="PLB65_07950"
     CDS             complement(2678..3856)
                     /locus_tag="PLB65_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78497.1"
                     /translation="MLKKLKEVFQGIFGIPLPESPMADKLLVPLTDNVKECLLFGSEI
                     AAKDAFLIQGSLSRFISECPEGYFLIGFWGHGINSHAFYYSRVDSWSKVLFRLGYGGA
                     YMDNALEAKYIKDFLIRYFDFERKISGQVKSFIAIDSMDEGYYSIELPDGKEYEIQES
                     LLRRPNFDDIFNHPYRPKEVDTMITKKSSWLWPHLESILKQKRSTLLNWRKQIPTQQG
                     RVKNFENWVLVELVNKLIENDEVLEVRTNGHFTDKKISVKDVENHLGRELKGSKSSAK
                     NLSADISVKMKKLNPVTGNNFIYNAEIKTGLSPLEILNDLLIVKYYSEVSVADKAEFG
                     WVVLLPEDEKAYASSLKTYKKICEKIETEYPDFTLLKSDKTNDESIIFCVAIHNLGAS
                     "
     gene            complement(4012..>4211)
                     /locus_tag="PLB65_07955"
     CDS             complement(4012..>4211)
                     /locus_tag="PLB65_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78498.1"
                     /translation="QQMKNVEMKVEDNMLTIKVDLTKEFGPSASGKTIIIASTEGNIS
                     IPEKDEIKIGLNVYKKKPVAV"
BASE COUNT         1249 a          901 c          687 g         1374 t
ORIGIN      
        1 cagcaatcat tcctgttgca gaaaaataca accgtgttcg gggaatgaaa ggaacaaaaa
       61 gataaattac agttacatgc tgtagatatt tacaaggctt ctttgtttaa atatttttgg
      121 cgcatgtagg ctatttgtat tactgatggt attgtaacac gataggccgt tcccgaaaac
      181 attggtatag tgagaataga tgttataacc catccgactg gcccagcaaa aatggctaat
      241 cctcttgcta atccagcatt tactccaaat gacaatcccc tggataaaat tgccttggca
      301 acactattag caacataaag agctattttg tatgatgtaa attcacctgc tattatagcc
      361 gattgtaacg cagccataat agcaaccgag gaagcgtgct tcaaattcaa tttcattcct
      421 tttgcaaatt ctcttttttg ctcatcagtc atcttctcca gtgaatcctc tatgattttg
      481 aggaacaggt tggcttcgat tttttctact gatgattttt tattaaaatt tacttttaat
      541 tttttacata catcagttaa tatttccctg tatgaaattc catgtcgcct aataatattt
      601 aaaattgtgt ctccaccata atattgcaat tctgtcgcta ttaaatccca tgctcgctta
      661 taattacctt tgcaattatt aaatctatca tcacttaata agtcggatgt aattctgtcg
      721 ttcccttttt tgtcttttgt aagaaaatca actaaaattg acagatcctc attatcacag
      781 aactgcaaaa attctaaatc gctgtcttgc ttatatttat tacccatttt acctccatta
      841 ctgaagtttt atacacgtag tattttatta caattttatc atatgataaa ttactaccac
      901 ttcattaaat actattacat cccagtttat ttatcaacct tcagtttcac aagagctatg
      961 aagcttttta tcaatcagac taattttatt gcaaaattaa aattatttaa acaaattaaa
     1021 cgcagactcc tgcgcttctc ctgtataacc ttttagcatt ctcttttaag aagctgcgcc
     1081 acaacagcta tccaccccag catcaacatc aaaggctgct gatatagttt ccgctatacc
     1141 gttaatatag caacactaac aacagcatca gctatcctcc atagcatcca cgcgtggtgc
     1201 tcatgagtag cagctagtag cagtagcagc ttcccaatca atttaatggg agtcattaca
     1261 gctgtggtta gaggtacgat catgccgaca tcaagttgct gggcagcttg tataagattt
     1321 ttttaggagt agctgattaa acagcagcta tactccctca agcttcttca atataggatc
     1381 atccgccaga aatatattgg tggtttccgt ctcgtcatca aaaacaagaa ctgccgatcc
     1441 gtctttaagt ttggatttta cctgtctgac cattgtttcc agggaagctt caatagcgcc
     1501 ataatccgtc ccgtttctgg aaatgaattc ttcaatgact cccttgaggg catgagcact
     1561 gagtttattg acggggatga tgtggatcga catttttagc tccgtgtcaa tcatcataaa
     1621 aactatattg ctcttttgcc gtttccagca accttaaaaa ggaattaaca tcctgcaaac
     1681 aatctaaacg cagactcctg cgctttccct gttccaccag gttaacatgc tcccggaaaa
     1741 atctgtccac caactgttcg tatatcatct gtgcccgttt tggaactacc ctgtttttgg
     1801 gacgcagata tttctgcaga tacaatgcgg ctgcttctat gtcgtagatt gactcgatgg
     1861 gagtcattga atcacttcct tccactgtca tgtaggcata atacccaact ctgctgccgg
     1921 tggccattat ttttcacgta tcattaaact ctgctgaaca tttgattaaa gaaaagatgg
     1981 aaaatgatcc ttgcaaactg tacttgccgc ttacaagtaa ataaatatca tttattaaat
     2041 cagcatatta taacaatatc cggtccgtcc tgaaattctg gcacgcaaat ttcattataa
     2101 taaaggcaaa taagcaaaca ccctcaagga ggtaacagat catgaaaaag acattaggaa
     2161 ccatcgtagc ggcggcagca gtagttcttt tgacggcaac ctttggtttt gcggagtatg
     2221 cggcaacggg agcagccaac ttcccgtatt tccagctggg ctgcctgatc atcggcggct
     2281 tgctgatggt agctttgaag aaaaaatatg aaaaaatgta cacagcggaa gtagtcggcg
     2341 ccttcgctct ttacaccatt ctgatggcgc tcttcaccaa tccggttatt gatatggtaa
     2401 aaatcattgt gacctgagaa taagtattcg gaagtagccg ctacaacaac aaacatgatc
     2461 tgtttattat ataacccctc aaggctccaa tagcttgagg ggttttttat ggttcatccg
     2521 gttgatgcgt cgtccagtta tcttttgcat ctaccaacca aaggatttta aaaagcctta
     2581 acattggaaa taacgacttg aaataaagta accgtgaaag taacaagcat atctataccg
     2641 catccatgca gatgcttagg tgggcagtat ttttgaacta tgacgcaccc agattatgta
     2701 tagcaacaca gaaaataatc gattcatcat tggttttatc tgatttcagt aaagtgaaat
     2761 ccggatattc agtttctatt ttttcgcaaa tttttttata tgttttcaaa gatgaagcat
     2821 atgccttctc atcttctggc agaagaacaa cccatccgaa ctcagcttta tctgcaacgc
     2881 taacttcact gtagtatttt acgattagta aatcgtttaa gatttctaac ggactaaggc
     2941 ctgttttaat ttcagcgtta tatataaaat tattacctgt cacaggattc agttttttca
     3001 tttttacgga aatatctgct gataggttct ttgcactgga ctttgatccc tttaattctc
     3061 tgcctaaatg attttcaaca tctttcacac tgatcttctt atctgtgaag tgaccattag
     3121 tacgaacttc tagaacttca tcattttcaa taagcttatt tactaattct acaagaaccc
     3181 aattttcaaa atttttaaca cgtccttgct gagtaggtat ttgtttacgc caattcaata
     3241 gggttgatct tttctgcttg agaattgatt ctaaatgtgg ccacagccac gaggattttt
     3301 tagttatcat ggtatccacc tccttaggtc tgtatggatg attaaatata tcatcaaaat
     3361 ttggcctcct aagtaacgat tcttgaattt catactcctt tccatcaggc aattctatgc
     3421 tataataacc ctcatccatg gaatcaattg caataaagct cttaacctga ccagaaattt
     3481 tacgctcaaa atcgaaatag cggatcaaga aatcttttat atattttgct tctaatgcat
     3541 tgtccatata ggccccacca taaccaagtc tgaataacac cttgctccat gagtccacac
     3601 gtgagtaata aaaggcatga ctatttattc catgccccca gaatccaatc agaaagtatc
     3661 cttctgggca ctcactaata aatcgtgaaa ggctaccctg aatcagaaat gcatctttcg
     3721 cggcaatttc agaaccgaat aataagcatt ctttgacatt atcagttaaa ggaaccaaaa
     3781 gcttatcagc cattggactt tcaggaagtg gaataccaaa aatcccctga aacacttctt
     3841 tcaatttttt cagcataaat aatatcctgc tattgttcca acgtttcgag atgtccgcta
     3901 taccatacca attcaatgtg ccccttgtta tttatttcaa catgaaactc acataaattg
     3961 tttctccatg tccattattt cttagaggaa gcaagaaccg gatgaatctt tttataccgc
     4021 cacaggtttc ttcttataaa cattcaatcc aatcttgatt tcgtccttct ccggaataga
     4081 aatgttgcct tccgttgacg caataataat ggttttacct gatgccgatg gcccgaactc
     4141 tttggtcaga tcaactttga tagtcagcat attgtcttcc actttcattt ctacgttttt
     4201 catttgttgc c
//
LOCUS       DAOSTH010000111         4189 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_19123_length_4189_cov_13.800194, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000111 DAOSTH010000000
VERSION     DAOSTH010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19123_length_4189_cov_13.800194"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2463
                     /locus_tag="PLB65_07960"
     CDS             <1..2463
                     /locus_tag="PLB65_07960"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOQ78499.1"
                     /translation="ECGSEIKALFLNHLEYSLIKDTTTVRNWDIYYALALSLRDLLIE
                     RWLRTQYEYRKQDVKKVYYLSMEFLLGRMLTNSLINLEVYNEVYDMLKQMGYRLEDII
                     ELEPDMGLGNGGLGRLAACFMDSLATQAFPAYGYGIRYDFGIFKQQIVQGYQVEEPDH
                     WRKNGCPWEINRPELTYRVRFGGKVITQTLPDGRIEYHWVDTEDVMAVAWDIPVPGYE
                     VDNVNNLRLWQATSSDEFDLEYFNSGDYVKAVEKKTISENISKVLYPNDNVHLGKVLR
                     LQQEYFFVSATLQDIFSHWKRDHNSFTDLPEKIAIQLNDTHPALAIPEMMRILIDEDR
                     LSFDSAWEITKRCFSFTNHTILPEALEKWSISLFEELLPRHLLLIYQINSVVMEEVIR
                     LYPGDIVKMRNVSIIEETNGKAIRMAQLAIHSAHKVNGVAKLHTKILCERIFPDLAEM
                     YPDKFHNITNGITPRLWLHTCNPQLASLITEYIGNGWIRNLEEISKIEQYIDNYDFRT
                     SFAEVKQINKNHLSKYIYRETGIRVQDNSLFYTQIKRLHEYKRQLLNVLGAIARYFQI
                     KDNPQGDFVPHTVIFAGKAAPGYYLAKRLIKLINNIGEVVNKDPDVKDRLKVVFLPNY
                     SVSLAEKIIPATDLSIQISTAGYEASGTGNMKFALNGALTLGTLDGANIEMSEEIGIE
                     NMFIFGLNAEQVRHLQQSGYNPYSYYENDPELKRVVDSLIDGSFEEGEKGLFLPIWKS
                     LMEEGDLYLNLVDFRAFMDAFDRVDTLYQNREQWITKAIINVSKMGRFSSDRAIMEYA
                     NQIWNIQPLPLVLNNGNNNSSV"
     gene            2460..4076
                     /locus_tag="PLB65_07965"
     CDS             2460..4076
                     /locus_tag="PLB65_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HOQ78500.1"
                     /translation="MNRIKTCLLFLCGCLYLFPLTAAIVINEVCYDPVGSDDGFEWIE
                     LFNNGNSSEQLEGAKIVSGGASFTLQYTIPFFELRPGRYLLIGGSSVPNAQLIYNFSF
                     QNGGSETDGICYISPDGIYTDTVLYDSPNTNQLIDDTNYPGIYFAPDVIAGNSLARII
                     DGLDTNNCAIDFIAEANPTPGLPNRRHCDYAFGQYNFNYDYGFAEIDLWLKNLSSITP
                     LISAELSVMQNDDLLAQQIISPIPALDSLWVNLAFYCNNYPLIIKLNLTDDPDSTNNI
                     LLIPINPDMEQGIYINEYLANPETNNQEWIELYCGIKQRGDYYIEDSSNSKIRFSLPE
                     NSGYYVICQYPEELLIRYPDCPIGSVILAESWTFLNNSGDLLVLKNESTTLDSISYTG
                     EEILKGISRERVLSDSTVFWQNCYNEIGGTPGLPNSILPQTELPDLGKVTLSGSPCNA
                     KAGEKITLAYNFPAETNRISCRIFDLSGRKICTIADYSLVNASGFVHWNGCKQDGTFA
                     PRGLYIILWESQNASGGKIMRKQFTAVLKG"
BASE COUNT         1297 a          677 c          853 g         1362 t
ORIGIN      
        1 gaatgtggaa gtgagataaa ggccttattt ctaaatcatc tggaatattc attaataaaa
       61 gatacaacca ctgttagaaa ttgggatata tattatgctt tggctctcag tttacgcgat
      121 ctattaattg agagatggtt acgcactcaa tatgaatatc gaaagcagga tgtgaagaaa
      181 gtctattatc tttcaatgga attcttgctg gggcgaatgc taaccaacag tttaataaat
      241 ctggaagtgt acaacgaagt ttacgatatg ttaaagcaga tgggttaccg cttggaagat
      301 ataattgaat tggaaccgga catgggttta ggaaatggag gtttaggacg tttagctgcc
      361 tgttttatgg attctttagc cacccaagct tttcctgctt atggttatgg aattcgttat
      421 gattttggca tattcaaaca gcaaattgtt cagggttatc aagttgaaga accagaccac
      481 tggcgaaaaa atggttgccc atgggaaata aatcgtccgg aactcacata ccgagttcgt
      541 tttggaggca aagttattac gcagaccctt cctgatggca gaattgaata tcattgggtt
      601 gatacagaag atgttatggc tgttgcttgg gatattcctg ttcctggtta tgaagtagat
      661 aatgtaaata atttacgtct ttggcaagca accagcagtg atgaatttga tttggaatat
      721 tttaatagtg gggattatgt aaaagcggtg gagaagaaaa ccatttccga gaacatcagc
      781 aaagtgctct atcccaatga taatgttcat ttaggtaagg tgttacgttt gcagcaggag
      841 tatttttttg tttctgctac gctccaggat attttttcac attggaaaag ggatcataat
      901 agctttacag accttccaga aaagattgct attcagttaa atgacactca tccagctctt
      961 gccattcctg aaatgatgcg tattttgatt gatgaggatc gtctcagttt tgattctgcc
     1021 tgggagatta caaagcgttg tttttctttt acaaaccaca caattttacc tgaagcttta
     1081 gaaaaatgga gcatttctct ttttgaggaa ttactgccgc gtcatctatt acttatttat
     1141 caaataaaca gtgtagtaat ggaagaagtt attcgcttat atccaggtga tattgttaaa
     1201 atgcgtaatg tctctataat tgaagaaact aatggaaaag caattaggat ggctcagtta
     1261 gcaattcata gtgcacacaa agtgaatggg gtagccaaat tacataccaa aattctatgt
     1321 gagagaattt tccccgatct tgctgaaatg tatcctgata aatttcataa tattacgaat
     1381 ggtatcactc ctcggctttg gctgcatact tgtaatcctc aattagccag tttaattaca
     1441 gaatatatag gaaacggctg gattaggaat ttggaagaaa ttagtaaaat tgaacagtat
     1501 atagataatt acgactttcg tacttccttt gctgaagtga agcagatcaa caaaaatcac
     1561 ttaagtaaat atatttaccg agaaacaggt atcagagtgc aggataattc tctcttttat
     1621 actcagataa aacgattgca tgagtataaa cgtcaattat tgaatgtgtt aggagcgatt
     1681 gctcgttatt ttcagataaa agataatccg caaggtgatt ttgttccgca tacagttatt
     1741 tttgccggaa aagcggctcc tggatattat ttagctaaac gactgataaa attgataaat
     1801 aacattgggg aagtggtgaa taaagatcct gatgttaaag accgtttgaa agtagtcttc
     1861 ttgccaaatt attctgtttc tctggcagaa aagattattc cggcaacaga tctttccatt
     1921 caaatttcta ctgcaggtta tgaagccagc ggaacgggaa atatgaaatt tgctttgaac
     1981 ggagctttaa ccctgggaac tttggatgga gccaatatcg aaatgtctga agaaatcgga
     2041 attgaaaata tgtttatttt tggcttgaat gcagagcagg taagacatct acaacaaagc
     2101 ggttataacc cctatagtta ttatgaaaat gatcctgaac tgaagagagt agttgattcc
     2161 ttgatagatg gcagctttga ggaaggcgaa aaaggtctct ttctgcctat ctggaaatca
     2221 cttatggaag aaggtgactt atatctaaac ttagttgatt ttcgtgcttt tatggatgca
     2281 tttgatagag ttgatacact ttatcaaaac cgcgaacagt ggataacaaa agctataata
     2341 aatgtatcta aaatggggag attctccagc gatagagcaa ttatggaata tgctaatcaa
     2401 atttggaaca ttcaaccctt acccttggta ttgaacaacg ggaacaacaa ttcttctgta
     2461 tgaacaggat aaaaacctgt ctcctctttc tttgtggctg tctttatctt tttcccttga
     2521 ctgctgccat tgttataaat gaggtttgtt acgatccagt tggttctgat gatggttttg
     2581 aatggataga actttttaat aacggcaata gttctgaaca actggaaggg gcaaaaattg
     2641 tctctggagg agcttctttt acccttcaat acactattcc attttttgaa ttaagaccag
     2701 gtcgttattt attgataggg ggttcctcgg ttcctaatgc tcaacttatc tataatttta
     2761 gttttcaaaa tggtggtagt gaaacagatg gtatctgtta tattagtcca gatggaattt
     2821 atactgatac tgtgctttac gattctccta atacaaatca actgatagat gatactaatt
     2881 atcccggaat ttattttgca ccagatgtta tagcaggtaa ttctctggca agaattattg
     2941 acggtttgga tactaataat tgtgcaatag atttcattgc agaagcaaat cctactcccg
     3001 gcttgccaaa ccggcgccat tgcgattatg cttttggcca atataatttt aattatgatt
     3061 atggctttgc cgaaatagat ctgtggttga aaaatcttag ttccatcact cctctaatat
     3121 cagcagaatt atctgttatg caaaatgatg atttactggc tcagcagatt atttctccca
     3181 ttcctgcatt agattcattg tgggttaatt tagcttttta ctgtaacaat tatcctctga
     3241 ttataaagct aaatttaaca gacgatccgg attctaccaa taatatatta ttgattccta
     3301 taaatccgga tatggaacag ggaatttata tcaacgaata tttggctaat cccgaaacca
     3361 ataaccagga atggattgag ctctactgtg gaattaaaca gcgaggggat tattatattg
     3421 aagatagttc caattccaag atccgttttt ctttgcccga aaacagtggc tattatgtta
     3481 tctgccaata tccagaggaa ttacttattc gctatccgga ttgtccaata ggttctgtta
     3541 ttttagctga gagttggaca tttctaaata attctggaga tcttttggtt ttgaaaaatg
     3601 aatcgacaac tttggattct atatcttaca ctggagagga aatattaaaa ggaatatccc
     3661 gtgaaagagt tttgagcgat tccactgttt tctggcaaaa ttgttataac gaaataggag
     3721 ggactcctgg tttaccaaat agtattttac cccaaacaga acttcctgat ttagggaaag
     3781 ttacgctatc tggaagtccc tgtaatgcta aagctggcga aaaaatcacc cttgcttaca
     3841 attttcctgc tgaaactaat cgtatttcct gccgtatttt tgatttgagt ggccgtaaaa
     3901 tttgtacaat tgcagattat tctttagtaa atgcttcagg atttgttcat tggaatggtt
     3961 gcaaacagga tggcactttt gcccctcgcg gactttatat tattttatgg gaatcgcaaa
     4021 atgcgagtgg ggggaaaata atgcgcaaac aatttactgc tgttctgaaa ggataattgc
     4081 tattttcttc ggttgagaag gattagaggg tttagaaaat tcaaaactct cagagcgaca
     4141 caatgatagc gatgggtgcg taaagcactc gaaacgagga ttttaggac
//
LOCUS       DAOSTH010000112         4083 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_19766_length_4083_cov_11.472939, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000112 DAOSTH010000000
VERSION     DAOSTH010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4083
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19766_length_4083_cov_11.472939"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="PLB65_07970"
     CDS             complement(<1..373)
                     /locus_tag="PLB65_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78501.1"
                     /translation="MELKSNLVIPSNFSHSKWITYFHTNSNIFSEKTEVMPKYEDDFF
                     NNTTTIGKLIFEDATQCGIYLIHVKRPLTDRTARKKQFNKAVEILKNEYVQAGLFVFY
                     DDNHSFRFSLIYPIYKGTKRSY"
     gene            complement(364..3714)
                     /locus_tag="PLB65_07975"
     CDS             complement(364..3714)
                     /locus_tag="PLB65_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOQ78502.1"
                     /translation="MSNFITNSGSKNLTTRLSELIQESDELKFLVGFFYFSGAMELID
                     SLQKNPDSILKILVGLSVDSSIYGIVEYAESSERTNEERKDMYQNSVLKAINNDEFDK
                     EQFYRQAYYVIEQISAGRIVIRKTLKSNHSKLYLFKLKESQVIRKNLFITGSSNLTKA
                     GLTDQNEFNVEISDYGFTEAEQYFDSLWDSAVHITEDDREKRRLISNLKNKTLLKSVH
                     PFDAYMYVVKTWLDTFYTEEYNTDIAYFLQKKGYRPLKYEIDAVWQGLSIIEEYGGVI
                     LADVVGLGKSIIASLIGWKMRKRGIIICPPGLIGDENANSGWKKYKEDFELYDWQVRS
                     GGDLDSIIEFLKRSKNIEVIIVDEAHRFRNGTTETYEKLKTICRGRKVILLTATPFNN
                     TPADILSLLSLFIVPKKSNITLSSNLVDLFRFYNGLFKDLAFITKNWKSKDPSKKDDA
                     RRKYNALFCDGDVDMKIVTGRAKLLSQQIREVIEPVIVRRNRLDLQNDPEYKKEIKEL
                     SKVEEPKEWYYKLTREQSEFYDRVIEKYFTDNLDYPDRFKGPVYKPYKYEGNIIDEKK
                     LTRQEQIDRLSQDNLYDILRRQMVKRLESSFGAFKQSVENLLNSYIQIKTFIQNSNGR
                     YILDRNLINKADVDDPESIDEILSQFIQNNQDREGRRNKIYDVNKFAFKDLFFADMDS
                     DIALFKKIKEEIDTLKLVENDPKLDTLLSNVGKILKSSHPKNEPPAKVVIFSEYTDTI
                     KHLKSSLNEYFNNRVLVITGGLSASILDDINTNFDAAYPTQYNDYDILLSTDKLSEGF
                     NLNRASTVINYDIPWNPVRVIQRVGRINRISRKVFDKLYIINFFPTEQGADIVRSRQI
                     AEQKMFMIHQTLGEDTRIFDPDEEPSPAKLFSKLQTNPYELEPESLYSKLKNLMHTWQ
                     DQYPEKVDALNNMPIRVKTAKSYSSDSLVMCFRKGKLFCVSQDFKDENNHYLPLPFEE
                     VLKRIECQPETEHLPLSNDFWINYNLLKREAEKFSYRQSQLSNSEKAYNFLSFLVGVP
                     DSDIQNWRSFILMLKEDIQIYGTLPEYTLKTIADWDNNGKPNFKHITQELEKLSQALG
                     NDYLNKVKFSLKEYEPQIIVAIENQKWN"
BASE COUNT         1261 a          725 c          645 g         1452 t
ORIGIN      
        1 tataacttct ttttgtccct ttatatatag ggtagattaa gctaaaacga aaggagtgat
       61 tatcatcata gaaaacaaac aatccagctt ggacatattc attttttagg atttcaacag
      121 ctttattaaa ttgctttttc ctagctgttc tatcggttaa gggtctcttt acatgtatca
      181 aataaatccc acattgggtt gcatcttcaa aaatcaattt acctatggta gttgtattgt
      241 taaaaaaatc atcttcatac tttggcataa cttcagtttt ttctgaaaaa atattagaat
      301 tagtatgaaa ataggtgatc cacttagagt gggaaaaatt ggaagggata acgagatttg
      361 attttaattc catttttgat tctctattgc tacaattatt tgaggttcgt attcttttag
      421 gctgaattta actttattca gataatcatt acctaatgcc tggcttaatt tttccagttc
      481 ttgggtaata tgtttaaaat tgggtttacc attattatcc caatcggcta tcgtttttaa
      541 agtatattct ggtaaggttc cataaatctg gatatcttcc ttcagcataa gaataaagga
      601 acgccaattt tgaatatcag aatcgggaac tccaacaaga aatgatagaa agttgtatgc
      661 tttctcagaa ttactaagtt ggctttgtct ataagagaat ttttccgctt ctctttttaa
      721 cagattataa ttgatccaaa aatcattgct taaaggcaga tgttctgttt ctggttggca
      781 ttcaattctt tttagtactt cctcaaaggg taatggtaaa taatgattgt tttcatcttt
      841 aaaatcctgg ctaacgcaga aaagtttgcc tttacgaaaa cacataacta aggaatctga
      901 actataggat ttggcagttt tcactctgat aggcatgtta tttaaggcat ccactttttc
      961 aggatattgg tcttgccagg tgtgcatcaa attttttagt ttggaatata gactctcggg
     1021 ttccagttca taaggattgg tttgtagttt ggaaaaaagt ttagcagggc ttggttcttc
     1081 atcaggatca aaaatgcggg tatcttcccc taaggtttga tgaatcataa acattttctg
     1141 ttctgctatt tgtctgctcc gaacaatatc tgcaccttgt tcagtgggaa agaaattgat
     1201 aatgtatagc ttatcaaaga cctttctact tattctattt attctgccga ccctttgaat
     1261 aacacgaact ggattccatg gaatatcata attgataact gttgacgcac gattgaggtt
     1321 aaacccttcg gagagtttat ccgtggaaag aagaatgtcg taatcattat attgtgtagg
     1381 atatgctgca tcgaaattag tattaatatc atccaatata ctagctgata atcctccggt
     1441 gataaccaga actctattgt taaaatattc atttaaagat gatttaagat gtttgatagt
     1501 atcggtatat tcagaaaaaa taacaacttt ggcaggtggt tcatttttcg gatgggaaga
     1561 ttttaatatc tttcccacat tgcttaacaa ggtatctagc ttggggtcgt tttccaccaa
     1621 ctttaatgta tcaatttctt cttttatttt cttgaacaaa gcgatatcag aatccatatc
     1681 ggcaaaaaac agatctttga aggcaaattt attaacatca taaatcttgt tccttctgcc
     1741 ttctctatct tgattgtttt gtataaattg actcagaatt tcatcaatgc tttcaggatc
     1801 atctacatcg gctttattaa ttaaattacg atcaagaata tagcggccat ttgaattctg
     1861 aataaatgtc tttatttgta tataagaatt tagaagattt tctacggatt gtttaaaagc
     1921 tccaaatgaa ctttccaaac gctttaccat ttggcgtctt aaaatatcat ataagttatc
     1981 ttgacttaac cgatcaattt gctcttggcg ggttagtttt ttttcatcaa ttatatttcc
     2041 ttcatattta tagggtttat atacaggacc tttgaaacga tcaggataat ccagattatc
     2101 ggtaaaatac ttttcaataa ccctatcata aaattcggat tgttctcggg ttaatttata
     2161 ataccattct ttgggttctt ctactttaga tagttcttta atttcctttt tatattcagg
     2221 atcgttctgt aaatccaatc ggtttctcct aacaataact ggttctataa cctctcggat
     2281 ttgttgtgag agtaatttag ctctaccagt tactattttc atatctacat ccccatcaca
     2341 aaaaagggca ttgtattttc ttcgggcatc atccttttta cttgggtctt tggacttcca
     2401 atttttagtt ataaatgcaa ggtctttaaa taagccgttg taaaaacgaa aaagatcaac
     2461 caaattgcta ctgagagtaa tattagattt tttgggaaca ataaataaac tcaatagaga
     2521 aagtatgtct gcaggtgtgt tattaaaagg agttgccgta agcaatataa ccttgcgtcc
     2581 tcggcaaata gtcttcagct tttcgtaggt ttcggtagtg ccgtttcgaa aacgatgtgc
     2641 ttcatcaaca atgatcactt caatattttt agaccgttta agaaattcaa taatgctatc
     2701 caaatcccct ccggaacgaa cttgccaatc ataaagttca aaatcctcct tatatttttt
     2761 ccatccggaa ttggcattct catctccaat taaacctgga ggacaaataa taatacccct
     2821 tttcctcatt ttccagccaa ttaatgaggc aataattgat ttacccaaac caacaacatc
     2881 cgctaaaatc acaccaccat attcttctat aatagataaa ccctgccaaa cggcatcaat
     2941 ttcatatttt aggggtcggt aacccttttt ctgcagaaaa taggcaatat cggtattgta
     3001 ttcttctgtg taaaaagtat ccagccaggt tttaacaaca tacatataag cgtcgaaagg
     3061 atgtacggat tttaggaggg ttttattctt tagattagat attaatctgc gcttttccct
     3121 atcatcttct gtaatatgaa ctgcgctatc ccataaagaa tcaaaatatt gttctgcctc
     3181 ggtaaaacca taatcagaaa tctctacatt gaactcattc tgatctgtaa gacctgcttt
     3241 ggttaaattg ctgctaccgg taataaataa attcttacga attacctggc tttctttcag
     3301 cttgaaaagg tagagcttgg aatgattgga ctttagagtt tttcggatga ctattctgcc
     3361 agctgaaatc tgttctatta cataatatgc ctgacggtaa aattgttcct tatcaaattc
     3421 atcattattt atagctttca gtacggaatt ctgatacata tctttccgtt cttcatttgt
     3481 tctttcagat gactcagcat attctacaat tccatatatc gaagaatcaa cacttaaccc
     3541 aaccagaatt tttaaaatag aatccgggtt tttctgtaaa ctatctatca gctccatcgc
     3601 acctgaaaaa taaaaaaaac ccactaaaaa ctttagttca tcgctttcct gtattagctc
     3661 tgaaagacgg gttgttaagt tcttgctccc cgaattagta ataaagttac tcattgatat
     3721 ttccttactt aaatataatt aacatagctt caaattatca gcataagcat tgattcaata
     3781 tcctgcataa acttatttag cctgaagatg actttgcgca tataagagga cacaatcaaa
     3841 ttcatcttcc aaaaataatg atagggttgc acactctatc tttttcacat ttacactatt
     3901 ttatagcctg ttttcaaaaa tttaaaacga aaatgatctt tccaactgct acaaaatagt
     3961 caagaacttt atctttctac tgtaacacac aagttatcag gggctaatga tgcaatggga
     4021 ctttactgta ttgctgtaac tcaaaaccgg cctttgggtt gactgtgtaa agcgaaggca
     4081 ggt
//
LOCUS       DAOSTH010000113         4065 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_19872_length_4065_cov_13.975561, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000113 DAOSTH010000000
VERSION     DAOSTH010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19872_length_4065_cov_13.975561"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..769
                     /locus_tag="PLB65_07980"
     CDS             176..769
                     /locus_tag="PLB65_07980"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOQ78503.1"
                     /translation="MIHNLLSHKKVILASASPRRQELFSLLGIAYQIIPAEIEEKVND
                     KLPQNQAMENALLKAKAVLNKVPDDALIVAADTLVAIDNIILGKPQDITEAKGYLSIL
                     SGRRHSVFTGICIYCNDTVNISYEQTFVQFAPLTEAEIDAYIATGEPMDKAGAYGIQG
                     YGAQFIIKVEGCYFNVMGFPIRLFYDLLKQILGKEEQ"
     gene            766..2229
                     /locus_tag="PLB65_07985"
     CDS             766..2229
                     /locus_tag="PLB65_07985"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOQ78504.1"
                     /translation="MKVLSNLYLWVNDRFTAEPQSLLVEKGIIIEQLPYPVPEQYKQL
                     YQQDFAGAYAYPGFIDTHTHSFEGGLYSLGVDLSIAKNIAEVLELLSAESAKKTKDEI
                     IFAWQLDETSLAEKRFPTVPELDSAVPGKPLLLRRIDGHSCMLNSFARKQIKDLDTDR
                     EILRGAENDRAVHYFHSCLTDDRILEAYQVSAKLGLQAGFCSLHTMIGNAQNSITHYE
                     LIRDNLDQFPLRFILYPQSFNIEAALNAGAQRIGGCILADGSIGSETAAVSEPYLNST
                     SRGVLYQSDEFWQQFINEASKHNLQVAVHCIGDRAIRQINNVYKAIPATEELRHELIH
                     CELTDDALIQEIAISKAVPVMQPNFDLLWGGENGFYAKKLGIKRSQIMNRFATLLKAG
                     ITITGGSDWYVTPLNAAQSINAAIHHHNPQEGLTIAQAIDIYTKNAAWLNFEEHCAGQ
                     IKPSYVADLSIYSQPVEKETSQLCYVVRKGEIKYAAQ"
     gene            2216..2800
                     /locus_tag="PLB65_07990"
     CDS             2216..2800
                     /locus_tag="PLB65_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78505.1"
                     /translation="MQHNNANGRAMDIKQIISLELTLHPHLQLIDIYKLVFQAYLGPA
                     HLISDKDEAAKRIMTEMLLHPEPYRPFKQDIGSCNGFFRLSLDLLLPNKSMRPEKAIT
                     ELYRKSYILAEYMQESVLDYTYNLDITKLWKEAIPVVQELAVFDITEWQEVNTIAESK
                     AIPRHSQQYKIHYNPKYRVLKYQFYNSIEQLLEK"
     gene            2817..>4065
                     /gene="gltX"
                     /locus_tag="PLB65_07995"
     CDS             2817..>4065
                     /gene="gltX"
                     /locus_tag="PLB65_07995"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOQ78506.1"
                     /translation="MQQVRVRFAPSPTGFLHIGGLRTALYNYLFARKEQGTFILRIED
                     TDQSRIVPGAVENLIASLHNLGLDFDEGPGKEGSFGPYIQSQRLNLYHQQAQYLLEKG
                     FAYPCFCSSETLTKMRKEQQEKGEFVKYDRRCLSLSKAEVESKIAAGEKYVIRLKMPD
                     NRRFRFYDLIRGEVEIDSSQSDDQVLLKSDGFPTYHLAAVVDDHFMQISHIIRGEEWL
                     SSTPKHIYLYECLGWEAPKWVHLPLILNPDRSKLSKRMNDVSVDNYLAKGYLKEAIVN
                     FVALLGWHPADERELFTLEELCEFFALEKVNKAGAIFDLTKLEWMNGHYLRSLPLEEI
                     ALRCKPLFLEKGYDISNPEKYNLCIAVARERCSLLNEIVDYCKMFYLPDTLTEEEEKI
                     LATPEAQKVLAWFVQKLPTLTNWE"
BASE COUNT         1258 a          738 c          845 g         1224 t
ORIGIN      
        1 gcaggaccag cgttaaagtt atgtacgcgt tccatttgta tcctcctgga tatatttttc
       61 ttctaaggaa ctataaaatc tgaggtcaag ctttcaggca agttattttt tagcaactga
      121 gaattttatt gactgaaaac cgctattttg ttctatgcag caaaggatta aagtgatgat
      181 acataatttg ttaagccata aaaaagtaat tcttgcttct gcttcacctc gcagacaaga
      241 attattttct ctcttgggta ttgcctatca gattattccc gcagagattg aagaaaaggt
      301 taatgataaa ttaccgcaaa atcaggcaat ggaaaatgcc ttgcttaaag cgaaagcagt
      361 gctgaacaaa gtgcctgatg acgctttaat tgtagctgcg gatactttgg tagcgataga
      421 taatattatc ttaggcaaac cacaagacat tacggaagct aaaggttatc tcagtatcct
      481 ttccggtcgc cggcatagtg tttttaccgg tatctgtatt tactgcaacg atacagttaa
      541 tatcagctac gagcaaacct ttgtgcaatt tgccccgcta accgaagctg aaatagatgc
      601 ctatattgcc actggagagc cgatggataa agcaggtgcc tatggaattc aaggttatgg
      661 agcacaattt ataatcaaag tagaaggatg ttatttcaat gtgatgggct tcccgattcg
      721 gttattttat gatttgctga agcaaatttt aggtaaggaa gaacagtgaa agttttatct
      781 aatctctatc tttgggtgaa tgacaggttt accgcagaac cccaatcttt attagtagaa
      841 aagggaatta ttatagaaca gctgccctat cctgttccgg aacaatataa gcagctctat
      901 caacaggatt ttgccggggc ttatgcctat ccgggcttta ttgatactca cactcacagt
      961 tttgaagggg gcttatattc ccttggggta gatttatcta tagccaaaaa cattgcagag
     1021 gttttggaat tgctctctgc tgaaagtgca aaaaagacaa aagatgaaat aatctttgcc
     1081 tggcagctgg atgaaacctc attagcagaa aagcgttttc ctacggttcc agaactggat
     1141 tccgctgttc ctggtaaacc attgctttta agaagaatag atggtcattc ctgtatgctc
     1201 aattcctttg cccggaagca aattaaggat ttggataccg atagggaaat tttgcgggga
     1261 gcagaaaatg acagggcagt tcattatttt catagttgct taacagatga taggattttg
     1321 gaagcttatc aggtatcagc taaacttggc ttgcaagcag gtttttgcag tttacataca
     1381 atgattggca acgctcagaa cagtattact cattatgagc ttatcagaga taacttggat
     1441 caatttcctt tgcgttttat cctctatcct caatctttta atattgaagc agctttgaat
     1501 gcaggagccc aaagaatcgg aggttgcatt ttagcagatg gctccatcgg ttcagaaacc
     1561 gcagctgttt ctgagcccta tttaaacagc acttcccgag gtgttctcta tcagagtgat
     1621 gaattctggc agcaatttat aaacgaagca agtaaacaca atttacaggt agcagttcac
     1681 tgtattggag atagagcaat tcggcaaata aataatgttt ataaagctat tcctgctacg
     1741 gaagaattac gccacgagct aattcattgt gagttaacgg acgatgcatt gattcaggaa
     1801 attgccattt ctaaagcagt tcctgttatg caacccaatt ttgatctgct gtggggtgga
     1861 gaaaatggct tttatgccaa gaaattaggc attaagcgta gccaaataat gaatcgcttt
     1921 gctaccttac taaaagccgg aattactatt accggtggtt cggattggta tgtaaccccg
     1981 ctaaatgcag cccaatcaat caatgctgct atccatcatc ataatccgca ggagggttta
     2041 accattgccc aggcaattga tatttacacc aaaaatgctg cctggctaaa ctttgaagag
     2101 cattgtgcag gacagataaa accatcttat gtagccgatt tatcaattta ttcgcaaccg
     2161 gtagaaaaag aaacatcgca gctttgttat gttgtaagaa aaggtgaaat taagtatgca
     2221 gcacaataac gctaacggca gggcaatgga tattaagcag attatttctc tggaactaac
     2281 tcttcatcct catttgcagt tgattgatat ttataaactt gtctttcaag catatctggg
     2341 acccgcacac ctgatttcag ataaggatga ggcagccaaa agaattatga ccgaaatgct
     2401 attacatcct gaaccctata gaccgtttaa acaggatatc ggttcctgta atggcttttt
     2461 tcgtttaagc ttggatttat tgcttccaaa caaaagtatg cgcccggaga aagcgataac
     2521 cgaactttac cgtaagagct atattttagc ggaatatatg caagaatccg ttttggatta
     2581 tacttataat ttagatataa ctaaactctg gaaagaggca atacccgttg tccaggaatt
     2641 ggcagtgttt gatataacag aatggcagga agtgaatact attgcagaaa gcaaagctat
     2701 tccaaggcat tcacagcaat ataaaattca ttataacccc aaatatcggg tgttaaaata
     2761 tcagttttac aattccattg aacagttatt ggaaaaataa ggataaggaa tttcttatgc
     2821 aacaagttcg tgtgcgtttt gctcccagtc caacaggttt tttacatatt gggggtttga
     2881 ggactgctct ttacaattat ctctttgccc gtaaagaaca gggaaccttt attctccgga
     2941 ttgaagatac agaccaaagt agaattgttc ccggagccgt agaaaatctg attgcttctt
     3001 tacataattt agggttggat tttgatgagg gaccaggtaa agaaggaagt ttcggaccct
     3061 atattcaatc ccaacgcctg aatttatacc atcaacaggc acagtattta ttggaaaagg
     3121 gctttgccta tccctgtttt tgttcatccg aaacattaac aaagatgcga aaagaacaac
     3181 aggaaaaagg ggaatttgtt aaatatgacc gtcgttgtct gtctttaagt aaagctgaag
     3241 ttgaaagcaa gatagccgct ggtgaaaaat atgtaattcg tttaaaaatg ccggataacc
     3301 gcagatttcg cttttatgat ttgattcggg gtgaagtaga aatagattcc agccagtccg
     3361 atgaccaggt tttactgaaa agtgatggat ttcctactta ccatctggca gctgttgtag
     3421 atgaccattt tatgcaaatt tctcatatca tccgagggga agaatggctt tccagcactc
     3481 ccaaacatat ttatctttat gaatgtttag ggtgggaagc gccaaaatgg gtgcatttac
     3541 ctttaattct taatcctgat cgctcaaaat taagcaaacg gatgaatgat gtatccgtag
     3601 ataactattt agctaaagga tatttaaaag aagctatagt taattttgtg gctttactgg
     3661 gttggcatcc tgctgatgaa cgcgaacttt ttactctgga agaactctgt gaattttttg
     3721 ctctggaaaa agtgaataag gcaggcgcaa tttttgattt gaccaaactg gaatggatga
     3781 atggacatta tttacgctct ctgcctttgg aagaaattgc cttgcgttgt aaaccacttt
     3841 ttttagaaaa gggttatgat atcagcaacc ctgaaaaata taacctctgc atcgcagttg
     3901 ccagagaaag atgttcttta ctgaatgaaa ttgtggacta ctgtaaaatg ttttatctgc
     3961 cggatacatt aaccgaagag gaagaaaaaa tacttgctac tcctgaagcg cagaaggttc
     4021 ttgcctggtt tgtccaaaaa ttacctaccc ttactaattg ggaag
//
LOCUS       DAOSTH010000114         4008 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_20232_length_4008_cov_10.130787, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000114 DAOSTH010000000
VERSION     DAOSTH010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20232_length_4008_cov_10.130787"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..824)
                     /gene="istB"
                     /locus_tag="PLB65_08000"
     CDS             complement(<1..824)
                     /gene="istB"
                     /locus_tag="PLB65_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HOQ78507.1"
                     /translation="MKVKLNQEIKEYCSILKLKGIRAHFEETMSEATDYEEFLHRLLT
                     FELEEKDKRSIECRIRNSHLPYKQYLEDIEIDCLPEDMQKKLPELATLNFIEKGKNII
                     MSGNPGTGKTMVSIALGLKACVAGYKVLFTTVPLLVTTLKECNSSKTLRYFENRFEKY
                     DLVIADELGYTSFDREGTDLLFNNLSLRAARKSTIITTNLSFERWVEVFGDPTVTSAM
                     IDRLTYKAILVDMEGDSYRLRETLRENGASFNFEDKTVLRIPEHPDGDSGNIRTRNR"
     gene            complement(826..2409)
                     /gene="istA"
                     /locus_tag="PLB65_08005"
     CDS             complement(826..2409)
                     /gene="istA"
                     /locus_tag="PLB65_08005"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOQ78508.1"
                     /translation="MTLTQKQKIILKHIDGMSNRSIAKELHMSKDTVNKYVTEYEKQK
                     AELLLMKPEADTEELIQAIVEKPKYNSDNREPKRVTPDMIKAIEECLELNEWRRVNGM
                     SKQQMKKIDIHTYLVDKKKFNISYSTVKRLVRSIEDKHKEAFIRQEYEHGDICEFDWG
                     TVKLDIGETGYDTYQMAVFTPAKSGYRYSMLFKAQDTPAFQQSHAEFFDYCKGNFKMM
                     VYDNMRVAVKKFVGLHEKEPTKALMELSIYYGFNFRFTNIARGNEKGHVERSVEYVRR
                     RVFSEPGNDKFDTLADANRFLAMGCTRLNSQPMSNGTIPLEVFEEEQKHLLPRLPKFE
                     SCIYSENRVDKYSTIMVSQNHYSVPDTLVGKMVSVKTFTDKIIVYYDNNLIAAHERSF
                     KLHDWKIDIFHYLRTLHKKPGALRGSTALLQADTQIKYIYEHYYSKDAKTFLQVLEIV
                     YEKGTDVVMKALRELELLSPMDMSADKVKVICEHKQEKKNGITVSYTDSLTEKSRSTL
                     SAYDRLAALQSGKIKKEVV"
     gene            2690..3001
                     /locus_tag="PLB65_08010"
     CDS             2690..3001
                     /locus_tag="PLB65_08010"
                     /inference="COORDINATES: protein motif:HMM:NF039453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6442 family protein"
                     /protein_id="HOQ78509.1"
                     /translation="MDKDRILEQVRTENIDEGVEHIKNKGLGIGYKIFLALSIIIIIF
                     NLFTGQKTYSVQSLFWGFIAAENHSLYKFSGDKSNKFGAICAGIACVVFLINHILYSL
                     G"
     gene            3007..3216
                     /locus_tag="PLB65_08015"
     CDS             3007..3216
                     /locus_tag="PLB65_08015"
                     /inference="COORDINATES: protein motif:HMM:NF024835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOQ78510.1"
                     /translation="MKKEYEFKNNLKEIRNKKGLTQTELAEMVGVSRNTISSIETGQF
                     SPTARLALVLCIALDKKFEELFYFE"
     gene            3425..3745
                     /locus_tag="PLB65_08020"
     CDS             3425..3745
                     /locus_tag="PLB65_08020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004634439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOQ78511.1"
                     /translation="MADLKERDMKNVTANLVGEVKTASFDWDGETINVTNFSLVEKRK
                     GKRHYTNCAAYGEWSEVAKKLKPGDLIHVFGYIKERRNNDKLYKNFIVKHMNKIEKEN
                     KEEK"
     gene            3747..3962
                     /locus_tag="PLB65_08025"
     CDS             3747..3962
                     /locus_tag="PLB65_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maff2 family protein"
                     /protein_id="HOQ78512.1"
                     /translation="MAFFTEGIAVLKTLVTAIGAGLGAWGVINLMEGYGNDNPGAKSQ
                     GIKQLMAGGGIVLIGMKLIPLLANTLN"
BASE COUNT         1216 a          743 c          761 g         1288 t
ORIGIN      
        1 cggtttcgtg tccggatgtt tccggaatcg ccgtccggat gttccggtat acgcaatact
       61 gtcttatctt cgaaattgaa ggatgccccg ttttctctta aggtttctcg aagccgatag
      121 gaatcccctt ccatgtccac caggatggcc ttataggtaa gccgatctat catggcactt
      181 gttactgtgg ggtccccgaa aacctccacc caacgttcaa aggataggtt tgtggtaatg
      241 atggtggatt tccttgctgc cctcaaagag aggttgttaa acagcagatc agttccctct
      301 cgatcaaagg aagtgtatcc taattcatct gcgatgacca ggtcgtactt ttcaaatcgg
      361 ttttcaaaat atcgcagtgt cttggaactg ttgcattcct taagtgttgt gaccaaaaga
      421 ggaaccgttg taaacaatac cttgtaaccc gccacacatg cttttaagcc caatgcaatg
      481 ctcaccatag tctttcctgt accaggatta ccgctcatga tgatattttt tcctttttcg
      541 atgaaattta aggtcgccaa ttccggcagt ttcttttgca tatcctccgg taagcagtcg
      601 atttcaatgt cttccagata ctgcttgtat ggtagatggg aattgcggat tcggcattca
      661 atggatcgtt tatccttctc ctccagttca aaggtaagca ggcgatggag aaattcttca
      721 taatcagttg cttccgacat agtctcctca aaatgtgccc ggattccttt cagtttcaat
      781 atactgcagt attcctttat ctcttgattt aattttacct tcatcctata cgacctcctt
      841 cttaattttc cctgactgta gtgctgcgag cctgtcgtag gctgacagcg tacttcggga
      901 tttctcagtt agagaatccg tataagacac cgttatgcca tttttctttt cctgcttatg
      961 ttcacatatc accttcactt tatcagcact catatccatg ggagaaagca gttctagttc
     1021 ccttagggct ttcattacta catccgtgcc tttttcgtaa acaatctcta acacctgtag
     1081 aaatgttttt gcatctttgc tataataatg ttcatagata tacttgattt gggtgtccgc
     1141 ttgaagcagt gcagtgcttc ctctcagggc acctggtttt ttatgtaagg tcctaagata
     1201 atggaagatg tcaatcttcc agtcatgcag tttaaagctc cgttcatgtg cagcaataag
     1261 attattatca tagtagacaa taatcttatc tgtaaaagtc tttacgctca ccatcttacc
     1321 cactaatgta tctggtacag aataatggtt ctggctgacc atgatggtgg aatacttgtc
     1381 cacccggttc tcagagtata tgcagctttc aaacttcggc agccgaggca gcagatgctt
     1441 ttgctcttct tcaaatactt ccaatggtat agttccattg gacataggct ggctgttaag
     1501 acgcgtgcac cccatggcaa gaaatcggtt tgcatctgca agtgtgtcaa atttatcatt
     1561 acccggttca ctaaataccc gccttcttac atattccacg cttcgctcaa catggccttt
     1621 ttcattaccc ctggctatat ttgtaaatcg gaaattaaac ccgtaataaa tagatagttc
     1681 catcagtgcc tttgtgggtt ctttctcatg cagccctaca aacttcttta cagccactcg
     1741 catattatca tagaccatca tcttaaaatt acctttgcag taatcaaaga attctgcatg
     1801 ggactgttgg aaggcaggtg tatcttgagc tttgaagagc atagaataac ggtagccgct
     1861 tttagccgga gtaaatactg ccatctgata ggtatcataa ccggtttctc ctatatccag
     1921 cttaaccgtt ccccagtcaa actcacatat atcaccatgt tcatattcct gtcgtatgaa
     1981 tgcctccttg tgcttgtcct ctatggatct tactaaccgc tttacagttg aataactgat
     2041 gttgaatttc ttcttatcca ccaggtatgt atggatatca atttttttca tctgttgttt
     2101 tgacatcccg tttactctcc gccattcatt taattctaaa cattcctcaa tggctttaat
     2161 catgtctgga gtgaccctct ttggctcccg gttgtcggaa ttgtactttg gcttctcaac
     2221 aattgcttgt atcagttctt cagtatccgc ttccggtttc ataagcaaaa gctcagcctt
     2281 ttgcttttca tactcagtca catatttatt caccgtatcc ttgctcatat gtagttcttt
     2341 ggcaatgctc ctgttactca taccgtcaat gtgtttgagt atgatttttt gcttttgtgt
     2401 caatgtcacc acttcctttc aggcctcctt tcagctgtta ccaaaaggat accatgtttt
     2461 attggaagtg acctggtttt caatgagcca tctgacctaa ttttaaatta ccataaacag
     2521 ctaggtaagg agtctataat aacaagattg taggtaataa ttatgaaggt aaaaacccaa
     2581 tatagttaat atataaaata tattatataa tttataaaaa cccttgacaa gtttacttgg
     2641 aaaatataca ataaagatgc aaagtaaact tggcgaggga ggaagcgaaa tggataaaga
     2701 tagaatttta gaacaggtaa gaactgaaaa tattgatgaa ggagttgaac atattaaaaa
     2761 taagggacta ggcataggtt acaagatttt tttagcacta agtattataa ttattatttt
     2821 taatttattt actggtcaaa aaacctattc agttcaatca ctattttggg gatttattgc
     2881 tgcagaaaac cattccttat ataaattttc aggagataaa tcaaataaat ttggggctat
     2941 atgtgctggt attgcctgtg tggtattcct tattaatcat attctttata gtttgggtta
     3001 gtaattatga aaaaagaata tgaatttaaa aataatttaa aagaaattag gaataaaaaa
     3061 gggctaaccc aaactgaatt agcagaaatg gttggagttt caagaaatac aataagttct
     3121 attgaaacag gacaatttag tcctacagcg agattagcat tagtactttg tatagcatta
     3181 gataaaaaat ttgaagaatt attttatttt gaatagtaat aaagaaatct agtagaagat
     3241 gatagtcagt agaatctatc atcttttttc atgtctagaa caaggagatc aatatgaaaa
     3301 ggataagaag tccaactaaa tagtttataa taaaaatata gaatagataa tagcgattgg
     3361 ataagaaaca agtccagtcg ctttttttat ttcaaaaaag aaaataggag gctaggaata
     3421 ggatatggca gatttaaaag aaagagatat gaaaaatgta acagctaatc tagtggggga
     3481 ggtaaaaaca gcttcctttg actgggatgg tgaaaccata aatgtaacaa acttttctct
     3541 tgtagaaaaa aggaagggta aaaggcacta tacaaattgt gcagcctatg gggaatggtc
     3601 agaagtagca aagaaactaa agccaggaga tcttatccat gtctttggtt atatcaaaga
     3661 aagaagaaac aatgacaagc tatataagaa ttttatagta aagcatatga ataaaattga
     3721 aaaagaaaat aaggaggaaa aataatatgg cattttttac agagggaata gcagttttaa
     3781 agactttagt tacagcaatt ggtgcaggtt taggggcatg gggagtaatt aaccttatgg
     3841 agggttatgg gaacgataac cctggggcaa agtcacaagg aatcaagcaa ctaatggcag
     3901 gtggaggtat tgttcttatt ggaatgaagc ttataccatt acttgcgaat acacttaatt
     3961 aagaaagagg tagataaaaa atgtttggac ttttcgacaa gataactg
//
LOCUS       DAOSTH010000115         3996 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_20307_length_3996_cov_12.743212, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000115 DAOSTH010000000
VERSION     DAOSTH010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20307_length_3996_cov_12.743212"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLB65_08030"
     CDS             <1..918
                     /locus_tag="PLB65_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgD immunoglobulin-like domain containing
                     protein"
                     /protein_id="HOQ78513.1"
                     /translation="NDIITVSMSYGIHPEGLQYNLAFNNRSIGIGAYVLYYTGLSYHP
                     TDVRYRLNGGELQTVTFSVNPVTESGDKSITVTINNLPELSKATYNLQIVLNDDSGQT
                     LPVVLSSFTATLMVQGKVRLDWVTQSASGLTGFRVLRNTVAEVSTAIAVSSLIEANNT
                     STAVPYSFIDNEIPCNGTYYYWLQIEGQDGSVSYYGSIAVEVTNGNNPDIPIPQITAL
                     NNPFPNPFNPSLTIPFDLSKDGRVTIKIYNLKGQLIKNLIDEDKKASNYRIVWDGKDN
                     NGHIVSAGTYIVRMNAPEYNSSRKIVMVK"
     gene            1360..2988
                     /gene="groL"
                     /locus_tag="PLB65_08035"
     CDS             1360..2988
                     /gene="groL"
                     /locus_tag="PLB65_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425343.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOQ78514.1"
                     /translation="MAKQMQYAHDARTSLKSGVDKLADAVKVTLGPKGRNVVLDRKYG
                     SPTITNDGVTIAKEIELEDEFENMGAQLCKEVAEKTHDTAGDGTTTATILAQAIIEEG
                     LKHVTAGVNPMYLKRGLEKATKVIVDKIKEYSKEIKSSEEIAQIAAISANNDPEIGAL
                     IAEAMDSVGREGIINIEEAKSIDTGLEKVEGMQFDRGYISPYFVTNAEKMIAELEDPF
                     ILIYDKKISGLKELLPILQEVSQNGRPLLIIAEDIEGEALATLVVNKLRGILNVVAVK
                     APGFGDRRKAMLEDIAVLTGATLISEEMGRKLDSATMSDLGRAKKIIVDKENTTIREG
                     AGDPEDVQARIKQIKAQIEETTSDYDKEKLQERLAKLSSGVAVLRIGAATETEMKEKK
                     ARVDDALHATRAAVEEGIVPGGGVTLIHAAKALKQMKGLSFEEKMAVDILSKALEKPA
                     YQIAANAGEEGAVVVEKLKGFRDIHMGFNAANGEYEDLFKAGIIDPAKVVRSAIQNAA
                     SIAGLMLTTECTITDIKEPEPAVPMPNPGMGDMY"
BASE COUNT         1321 a          652 c          815 g         1208 t
ORIGIN      
        1 aatgatatca ttactgtttc tatgagttat ggcattcatc ctgagggttt gcagtataac
       61 cttgccttta ataatcgtag tatcggcatt ggagcttatg ttctttatta tacgggattg
      121 agttatcatc cgactgatgt tcgttatcgt ttgaatggtg gtgaattaca gactgttact
      181 ttcagtgtca atccagtaac ggaatctggg gataaatcaa taacagtgac aataaataat
      241 ttacctgaac taagcaaagc aacttataat ctgcaaattg ttcttaatga tgatagtggg
      301 cagacccttc ctgtagttct ttcctctttt acagccactt taatggttca gggtaaagtg
      361 cgtctggatt gggtaactca atcggcatca ggtttaactg gctttagagt attgcgtaat
      421 actgtggcag aagtttctac tgctatagca gtaagttctt taattgaggc aaataatact
      481 tccacggctg ttccctattc ctttattgat aatgaaattc cctgcaacgg cacttattat
      541 tattggctac agattgaagg acaggatggc tctgtatctt attatggctc aattgctgtg
      601 gaagtgacca atggcaataa tcctgatatt ccgattccac agattacggc tttaaataat
      661 cctttcccca atcccttcaa tccttctttg accattcctt ttgatcttag caaggatggc
      721 agggtaacga ttaaaattta caacctgaaa ggtcaactga ttaaaaacct gatagatgaa
      781 gataaaaagg catcgaacta tcgtatcgtt tgggacggga aagataataa cgggcacatt
      841 gtttctgccg gaacttatat tgtgcggatg aatgcccccg aatataattc ttctcgtaag
      901 attgtgatgg ttaaataaca caatagtaca tttctgcaag gtgcgtagtt tttttacgca
      961 tcttttattt tatctctgaa aaatggaaga ttagcaattc atagtatcgc taaacaatta
     1021 ggttccttgc tatgtttagt tacttccaat tgtggaatag tgcaattaac taatattatc
     1081 ttgcctgagg atagttttga gggatgagtt ctttaagtaa atttcgggca aaaatgaaag
     1141 aaaaaatgag cagcagtttt agcacaactc tctaatatcc ctatttcttg taataaaaaa
     1201 gagtagttag accatttagg tgtaagtaaa gaatagggga gagcataaaa aaataaattt
     1261 gcgcttgaca gttttctgac tataataata ttagcactca aatgaattga ctgctaagaa
     1321 aacgaaaaca aaataattaa tattatggag gaaaacaaga tggcaaaaca aatgcagtat
     1381 gcacatgacg cccgcacaag tttaaaaagc ggtgtggata aactggctga tgccgtaaaa
     1441 gttacccttg gtccgaaggg tagaaatgtt gtattggata gaaaatatgg ctctcctaca
     1501 attaccaatg acggagtgac cattgccaaa gaaattgagc tggaagatga atttgaaaac
     1561 atgggtgctc agctttgtaa agaagttgct gaaaagactc atgacactgc tggtgatgga
     1621 acaaccacag ctacaatttt ggcgcaagct attattgaag aaggtttaaa acatgtaacc
     1681 gccggcgtta atccaatgta tttgaagcgt ggtttagaaa aggcaactaa ggttatagtg
     1741 gataagatta aagaatatag caaagagatt aaaagtagcg aagaaattgc tcagattgcg
     1801 gctatttctg ctaataatga tcctgagatt ggagctctta ttgctgaagc tatggatagt
     1861 gttggtagag aaggaatcat caatattgaa gaagcaaaat ctattgatac tggcttggaa
     1921 aaagtagaag gaatgcaatt tgatcgtggt tatatttcac cctattttgt cactaatgca
     1981 gaaaagatga ttgccgagtt ggaagatccc tttattctga tttacgataa aaagatcagt
     2041 ggactcaagg aattgctgcc tattttgcag gaagtttctc agaatggccg tcccttattg
     2101 atcattgcag aggatattga aggtgaagct cttgctactt tagtagttaa taaacttcgc
     2161 ggaattctga atgtggtagc ggttaaagct cctggttttg gagatcgcag aaaagctatg
     2221 ttggaagata tcgcagttct tactggagca actcttattt ctgaagagat gggcagaaaa
     2281 cttgatagcg ctacaatgag tgatttaggg cgtgccaaga agataattgt ggataaagaa
     2341 aataccacta tccgtgaagg tgctggtgat cctgaagatg ttcaagccag aattaaacaa
     2401 ataaaagctc agatcgaaga gactacctcc gattacgata aagaaaaact tcaggaacgt
     2461 cttgccaagc tttccagtgg agtagccgtt cttagaatcg gagcagctac cgaaactgaa
     2521 atgaaagaga aaaaagctcg tgttgatgat gctttacatg ctacccgggc tgcagttgaa
     2581 gaaggaattg ttcccggtgg tggagttact ttaattcatg ctgcaaaagc ccttaagcaa
     2641 atgaaagggc tctcttttga agagaaaatg gcagtggata ttctttctaa agcgttagaa
     2701 aaacccgcat atcaaattgc tgctaatgct ggagaagaag gtgccgtggt agttgaaaaa
     2761 ctaaaaggtt tccgcgatat ccatatggga tttaatgctg caaacggtga atatgaagac
     2821 ctcttcaaag ccggaattat tgatcctgcc aaggttgtgc gttctgctat ccaaaatgca
     2881 gcttccatag ctggtttgat gttgactact gaatgcacaa ttaccgatat caaagaacca
     2941 gaacctgcag ttccaatgcc caatccaggt atgggtgata tgtattaatt tcacaataaa
     3001 tcatataaca agaatgggcc tctcaatcca ggagaggctc ttttatttaa taagcaatta
     3061 tcaataaaaa aaagactcct gcagattgcg ctgcaaatta taacattaga tctgaagtga
     3121 acaagatttg gctagtatag ttaaaaatta tcccctctat agtttttatt cctataagac
     3181 acatataagc atcaattaca attggaaaga ttaaagtaac aatataaaaa tatcacgata
     3241 attcccctct tgaaactgca ttataccaac tgtaacaatc aattttgtaa taaaatccca
     3301 ataattttgt ctcctattat ccttgctcga ggccattaat ctatcgttta acaggcctta
     3361 atcaagcgtt aataattaac gcttgattaa ggctagatta acacttgatt aacgctttcc
     3421 attaagagcg agtagttgaa gtcgaagccg atagacagtt tatccaaaag taataaatac
     3481 ctgatgacag atagatatat taggaaagta cttaatatca atccataatt ttaaagttcg
     3541 gggaaaatga gctaacctgt tcttgcttgg caaataaact gaagttaatc agcggacttt
     3601 gagcttgcct gtttttagga taattttatt caacgattca gttgctgaaa atgtctataa
     3661 actatgtggt tacattattg ggattttttg gttagcggga aatttctgat gaattgtaga
     3721 aacagaatat aacttttttc ttaggaactg aacttaataa aaaagggggg tacttttttc
     3781 ctttactcgg caaagagatt ctgcactttt ttcttgacaa caaatcttaa tgcagcattt
     3841 ggattaaaat ctatacatat aaagctaggg aacaactaat gaaagaaggt ttaatacaaa
     3901 aaaatgacta tcattccata ttcttttcag atcgagcaga ggaatgtgga agtgagataa
     3961 aggccttatt tctaaatcat ctggaatatt cattaa
//
LOCUS       DAOSTH010000116         3922 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_20832_length_3922_cov_21.678304, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000116 DAOSTH010000000
VERSION     DAOSTH010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20832_length_3922_cov_21.678304"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            360..2513
                     /locus_tag="PLB65_08040"
     CDS             360..2513
                     /locus_tag="PLB65_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78515.1"
                     /translation="MKKIFLLLMLLIVCILGYAQSAELTSTNQIGDAVANAGLTKKRL
                     FTFQLKMTGGGGTLTDVTFSTSGNYTANELNKLELYYNTANTFTSATSIKSLTSNLET
                     GSHIFSGLNKALGNGNHYFWITADIAQSIIPNHTISVPAISTTDITFSETFYKGGSTI
                     AGGTQYLEYVVVTENFDGTWPPSGWTFYGAARNSPAYSGAYSLELQPAGDYAYTRKVN
                     TPGLITFFAMVNTGSQYSFEIQCSTDTTNWTNITTYSNTANSLTRSFKFYSIDVHAAT
                     YTNVYFRFYLASGNGDIHIDDVNITLRSPNTQASNVTFSDIGPSRFTVSCTAGSAAQR
                     IMFMKEGNVSPVDADNGVFYTASTNWNDKGSQIGSSGFYCIYRGTGTSVSVTGLTKNT
                     TYTVEVMEYNYSDVANTYIYMPSPAYNHTTTASTDPPSAIIFDSVTSSSFNLSWSTTT
                     GLSYVLFLKEDTSVYPPTPSGTYTASSDWSNKGSQLETSGFYCVYNDTGNNVTVTNLQ
                     SSTRYYAVIFTLHDGTYSSGCACGGEVPTATKAADYFASIASGNWAQVHTWDSTNHSW
                     AGNTTWKSSRDGTHWNDATAKPTSSAAKVYIESGHIVTLTANESCNNLHFDGGWIQLG
                     NFNLTINSYDSASGNPQFIYNGTGAPQMTTQGTNYIVSVTSQSITSLPSEVYTLRINL
                     TGSDTLSVPLPNDVTVTNLAFDNGGLRMNSHTIKY"
     gene            2559..3476
                     /locus_tag="PLB65_08045"
     CDS             2559..3476
                     /locus_tag="PLB65_08045"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78516.1"
                     /translation="MNIRYTEDVNIVGSNTSLPITWYTSGYVTDVFQATMYFPSSLTT
                     STSLRLWLRNNATKGWYLKGEYPVQGEGDTKYIVADNLTSPDMSGIVFFDFTLSEVNQ
                     TLPVELSSFIVNVNYQNYVQILWVTQSETELSGFRIYRGVSDDLSSALDLGIFIPATN
                     TSEPKYYVYTDKEIDASGTYYYWLECIDLNANSTFYGPKELIFHPGVDGNNIPVLEGI
                     NSIYPNPFNPDTTIRFGVLESSTVKAIVYNNRGQKVCEPINGYYDKGTYSYIWNATDS
                     YGRRLSNGIYILSLQVGSKTYIRKMAILK"
BASE COUNT         1245 a          718 c          689 g         1270 t
ORIGIN      
        1 gaataaaatg cagataaacg gatcaacagg atttaattaa aaataattat gatccgctta
       61 aacccattgt cattcctacg aaggcaggaa tccattatga aaataaatta ttttttttaa
      121 tcctctcaat cctaatcagg aatacaaaaa aaaatatttt tttactgcct taaataactt
      181 tattccttta tgttacatca tttaagaaag aaaaattcca ttaacattga aaaaaagcat
      241 ttgacatatc ttgaatattg attattttaa tagagcataa tagataaaag tatatatgaa
      301 ttagttactg cttatacgat aaaagttata catacggaat aagcgggaag gagttaaaaa
      361 tgaaaaaaat atttttgctg ttaatgctgc ttatagtttg catcttgggc tatgcacaaa
      421 gtgctgaatt aacttctact aatcaaattg gagatgcagt tgccaatgca ggattaacaa
      481 aaaagcgtct cttcaccttc cagcttaaaa tgactggtgg tggtggcact ttaacagatg
      541 taaccttctc tacctccggt aattacactg caaatgaatt aaacaaactg gagctttatt
      601 acaataccgc taatacattt accagcgcta cgagtattaa atctctaact tctaatttag
      661 aaaccggttc acatattttc tctgggttaa ataaagcttt aggtaatggt aatcattatt
      721 tctggataac ggctgatatt gcacaatcaa ttattcccaa tcatactatt tctgttccag
      781 ctattagtac taccgatatc actttttctg aaacttttta taaaggcggt tcaactattg
      841 caggcggaac tcaatatctg gaatatgtgg tagtaacaga aaattttgat ggaacctggc
      901 ctcccagcgg ttggactttt tatggagctg ccagaaattc tcctgcttat tccggtgctt
      961 attctcttga gcttcaacct gccggtgatt atgcttatac caggaaagtt aatacacccg
     1021 gacttataac tttttttgct atggtaaata ctggtagcca gtattctttt gaaattcaat
     1081 gtagtaccga taccacaaat tggactaata ttacaaccta ttctaatact gcaaattctc
     1141 ttaccagaag ttttaagttt tactctattg atgttcacgc cgctacttac actaatgtct
     1201 atttcagatt ttatctggca agcggaaatg gagatatcca tattgacgat gtgaacataa
     1261 ctttgcgcag ccccaatact caagcttcca atgttacttt ttcggatatt ggaccaagca
     1321 gatttacagt aagttgtaca gctggaagtg ctgctcaacg cataatgttt atgaaagaag
     1381 gtaatgttag tccggttgat gcagacaatg gtgtttttta tacagccagc actaattgga
     1441 atgataaggg ttcccaaatt ggttcatccg gattttattg tatctataga ggcactggaa
     1501 caagtgtaag tgttaccggt cttacaaaaa atacaactta cacagttgaa gtgatggaat
     1561 acaattacag cgatgttgcc aatacctata tttatatgcc gtcccctgct tataatcata
     1621 ctacaactgc atctaccgat cctccttccg ctattatttt tgatagtgta acttcttcca
     1681 gttttaatct ttcctggagc actactaccg gcttatctta tgttttattt ctaaaagaag
     1741 atacttctgt ctatcctcct actccctctg gaacttatac tgccagtagt gattggagca
     1801 ataaaggatc tcaactggaa acatcaggat tttattgtgt ttacaacgat acaggaaata
     1861 atgtaacagt taccaattta caatccagca caagatatta tgcagttatt tttactttac
     1921 acgatggaac ttattcttct ggttgtgctt gtggaggtga agttcctacg gctacaaaag
     1981 ctgctgacta ttttgccagt atagcaagtg gaaattgggc tcaagtgcat acatgggata
     2041 gcacaaatca tagttgggct ggaaatacaa cctggaagtc ctcaagggat ggaactcact
     2101 ggaatgatgc tacagcaaaa cctacttcca gtgcggctaa agtttatata gaatcgggac
     2161 atattgtaac tttaacagca aatgagagct gtaataattt acattttgac ggtggttgga
     2221 tacaacttgg taacttcaat ttaaccatta attcatatga ttcggcaagt ggaaatccgc
     2281 agtttattta caatggaact ggtgcccctc aaatgacaac gcagggaacc aattatattg
     2341 tctccgtaac ttctcaaagt ataaccagtt taccctctga agtatatact ttaagaatta
     2401 atcttaccgg ctcggatact ttatcggttc cgcttcctaa tgatgttaca gtaacaaatt
     2461 tggcttttga taacggtggt ttaagaatga attcccacac catcaaatat taatataaat
     2521 atgccgatat atattcaaag aatgctatat tttatagtat gaatatcaga tataccgaag
     2581 atgttaatat tgtaggaagt aacaccagtt taccaataac atggtatact tccggttatg
     2641 taaccgatgt ttttcaggca actatgtatt ttccgtcttc tttaacaacc tccactagct
     2701 taagattgtg gctgcgcaat aatgctacca agggttggta tttgaaaggt gaataccctg
     2761 ttcaagggga aggtgataca aaatacattg tagcagataa tttaaccagt cccgatatga
     2821 gtggtattgt cttttttgat ttcactcttt cggaagtaaa tcaaacccta ccggtggagc
     2881 tatcttcctt tattgtaaat gttaattacc agaattatgt tcaaatatta tgggtaacgc
     2941 aaagtgaaac ggagctttct ggttttcgga tttatcgtgg tgtctcggat gatttatctt
     3001 ctgctctgga cttaggcatt tttattcctg ccaccaatac atccgagccc aaatattatg
     3061 tttatacgga taaagaaata gatgcttcgg gaacttatta ttattggctg gaatgtatag
     3121 atctaaatgc taacagcact ttctacggtc ctaaagaatt aatcttccat ccaggtgttg
     3181 atggcaataa tattcctgtt ttagaaggaa ttaacagtat ctatcctaat cccttcaatc
     3241 ccgatactac tattcgtttt ggggttttag aatcctctac ggttaaagcc attgtctaca
     3301 acaaccgggg acaaaaggtt tgtgaaccaa taaacggata ttatgataaa ggcacctatt
     3361 cctatatctg gaatgcaact gacagttatg gcagacgctt aagcaacgga atttatatct
     3421 taagcttaca agttggctct aaaacatata tccgcaaaat ggctatactg aagtaatgga
     3481 gaattgaaaa ttaagtcagg agtcgtcact gctgaaataa attttaccct acctacaaac
     3541 ttatttggca gtgaggactc gtgacggtta gaaaatgtgg tcttggtgct ttagtttatc
     3601 aggattcctg cttttacagg aatgactaag acaagacatc ctaaaaaagt tgggcttaaa
     3661 ttacagaaaa tatccagtaa attcagttat ccagtcatct gtgttctatc atcctcagtt
     3721 aaaatcagct tgacacaaac ccttgttaat ccataaatgt tttcaataaa gttattcacc
     3781 ctatcaggag ttttaatgaa aaaacggtta tttccggttc ttctttgttg catgctgatt
     3841 ttcagtgctt gctctcaaaa tgcccgactt tccaaaaaga cccaaaagaa cttaaacaaa
     3901 ggtaattacg aagaagcaat tc
//
LOCUS       DAOSTH010000117         3860 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_21235_length_3860_cov_14.083311, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000117 DAOSTH010000000
VERSION     DAOSTH010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21235_length_3860_cov_14.083311"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..629)
                     /locus_tag="PLB65_08050"
     CDS             complement(138..629)
                     /locus_tag="PLB65_08050"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOQ78517.1"
                     /translation="MKKITTILFMLLLVSFALAEVMPDFKLPDAAGKNITLQELLGKG
                     PVLIDFWADYCKPCKDAMPQLNTLAEKYDSLTVVMISIDPPKNLPKAKNYLKSKNYKF
                     ITLFDSEKILAKKLGVVNPPHTFILNKTGEIVYSHIGYEPGVENEYELNIRNLLGLQN
                     EAE"
     gene            complement(641..1750)
                     /locus_tag="PLB65_08055"
     CDS             complement(641..1750)
                     /locus_tag="PLB65_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78518.1"
                     /translation="MMKKIVYAIMLLALLGIFSCDRFDHSFKPLEQVDFGTELFVPLQ
                     EAYNNISASDLTPVMAFYAEDYNHYGVNKSQWETMLHNMLSEISDPVIEVTLVKASLQ
                     NETNALANWRLKISDSNKAIIADSLYVGERLAKVNGKWLLRGNQCGCTPEVPTQKVIV
                     EYVTNVGCSYCPAVEELLHNLSSEFGDNFIYLTHQLSGPVAINDPLYVYYSAFSAPVS
                     IIQGKHKLASGTQEILNQYYPLVQTELAISNPMDYNIMSALVTGNSISGSVKLTPTTD
                     NFSQEELILNLVIIDRVSTANNAVGEPLTNVVIGRKRIDISSADLNNPVTFDFTANVD
                     IPDDAALVVFAQKTPTTFANNATIYSGLQYPLTVR"
     gene            complement(1769..3598)
                     /locus_tag="PLB65_08060"
     CDS             complement(1769..3598)
                     /locus_tag="PLB65_08060"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HOQ78519.1"
                     /translation="MNRGYKLLLALCLIMLIQPFFAQTVKFLLSPDVLKPGEKGVIKA
                     TLTIPEGKKQTVNPKNPEYFYLEASHPDLSFGKVEYPKPTNIISDEEWNYHPKVTLTL
                     PFTVKQTAKAGNKQVEVLLSYNLCYESGMCDPPEEATKKLSFQIITAEAAEGVVSEET
                     EATENIEKGETSPVSEVDRAATEPIAKRSLGEILKYILFAFLGGIILNITPCVLPILP
                     IRIMSIMNQAQKDRTKVLHHILVYTFGVLISFTIMAVIFIALQKAGESVGWGLQNQNP
                     GFVVTLMAIVFVFALSLLGVFEITVPGMNTANKATSKGGYSGSFFGGIFAFLMAISCT
                     GPFLGAALPFALALPSVLMLIFFLTIGLGFAFPFLLIGIFPKALKIIPKPGNWMIIFK
                     EVMGFVLLFIVYTQLKTLLLLTDGNYLLNVLWFLIILGFASWLYGRFVRVENSKLIQW
                     LFTLISIALVIFAAITYLPIPKADKAEVQVQSGELIPAPNAPEGWYVFSEDILNKALK
                     ENRAVFLDIGAAWCKNCMTNEKTVLFTDTMMQEFKQKNVLLLRGDFTKKDETLLSWIK
                     KHNRAGVPFNALYIPGEEPYIFGELLSKDEVIDALKKIQAKTE"
BASE COUNT         1241 a          743 c          692 g         1184 t
ORIGIN      
        1 gtgacttcgg tgacttcggt ggctaaatat tcttcttaaa tcctcgtttc taaaactcaa
       61 tcctctaaat ccttaaaatc ctgttcatcc catacctatt ccatctgcga aatctgcaaa
      121 atctgcgtga aaatatacta ttccgcttcg ttttgaagcc caagcaaatt acggatattc
      181 aattcatatt cattttctac accgggctca tagccaatat gggaataaac aatttctccg
      241 gttttattta gaataaaggt atgcggagga ttcaccactc ccaatttttt ggctaatatc
      301 ttttcggaat caaacagagt gataaactta tagtttttgc ttttcaggta gttttttgct
      361 tttggtaaat ttttgggggg atcaatagag atcattacaa cggtaagcga atcatacttt
      421 tctgctaaag tattcagctg aggcattgca tctttacagg gtttgcaata gtccgcccaa
      481 aaatcaataa ggacgggacc ctttcccaaa agttcttgta gggtaatatt ttttccagct
      541 gcatcgggaa gcttgaaatc tggcataact tctgccagcg caaaagaaac caaaagcagc
      601 ataaagagga tagtagtaat ttttttcatt cgctatgctc ctatctaact gttaacggat
      661 attgtaagcc actataaatt gtggcgttat tggcaaaggt ggtaggtgtt ttttgggcaa
      721 aaacaactaa cgctgcatca tctggaatat caacattggc agtaaaatca aaggtaacag
      781 ggttatttaa gtctgctgaa ctgatatcta tgcgttttct gcctataaca acattggtaa
      841 gtggttcacc aacagcatta ttggcagtag aaacacgatc tataattacc agatttaaaa
      901 tgagttcttc ttggctgaaa ttatctgtag tgggggtaag ctttacgctt cctgagatcg
      961 aatttcctgt taccaaagca ctcattatat tataatccat cggattggaa atagcaagtt
     1021 cagtttgtac gaggggataa tattggttta gaatttcttg cgttccgctg gctaatttgt
     1081 gttttccttg aattatggaa acaggagcgc tgaaagcact gtaataaaca taaagtggat
     1141 cgttaatagc aaccggaccc gatagctgat gcgttaaata gataaagtta tctccaaatt
     1201 cagagcttaa attatgcaga agttcttcca cagcagggca gtaactacat cctacattag
     1261 taacatattc tactattact ttttgggtag gaacctcagg ggtgcatcca cattgatttc
     1321 cgcgtaaaag ccattttccg tttactttgg ctaaacgctc tcctacatat aaactatcgg
     1381 caataatagc tttattgcta tcgctaattt ttaatcgcca gtttgccaaa gcattggttt
     1441 cattttgtag agaggctttt accaaagtaa cttctataac gggatcgcta atttcagaaa
     1501 gcatattatg cagcattgtt tcccactggc ttttattaac tccatagtga ttataatcct
     1561 ctgcataaaa agccatcacg ggagttaaat ctgaggcgct gatattgtta tatgcttctt
     1621 gcagaggaac aaaaagttct gttccaaaat ctacctgctc gagaggttta aaagaatgat
     1681 caaagcgatc acaggagaaa attcctaaga gcgctaagag cataatagca taaacaattt
     1741 tcttcatcat aatccttctt gtcttatcct attctgtttt tgcctgaatc ttttttagag
     1801 catcaataac ttcatctttg gaaagcagct cgccaaatat atatggttct tcaccgggaa
     1861 tgtataaagc attgaaagga acaccagctc tattatgttt ttttatccaa gaaagcaggg
     1921 tttcatcctt tttagtgaaa tcacctcgta gcagtaaaac atttttctgc ttaaattcct
     1981 gcatcatcgt atctgtaaat aggactgtct tttcattcgt catacaattt ttacaccagg
     2041 ctgctccgat gtcaagaaaa acagctctat tttcttttag tgccttattt aaaatatctt
     2101 cactaaacac ataccaacct tctggagcat taggagcagg aataagttca ccactttgaa
     2161 cctgtacttc tgccttatct gctttaggga taggcaaata agtgattgcg gcaaaaataa
     2221 ctaacgcaat cgagatgaga gtaaacaacc actggataag cttactgttc tccactctta
     2281 caaacctgcc ataaagccaa ctggcaaaac caagaataat taaaaaccat aaaacattca
     2341 gtagataatt accatcagtt agcaacaaaa gggtttttaa ctgggtgtaa acaataaaga
     2401 ggagaacaaa acccatcacc tctttgaata taatcatcca gtttccaggc ttggggataa
     2461 ttttcagtgc cttggggaaa ataccaatca ataaaaacgg aaacgcaaat cccagaccaa
     2521 tagtgagaaa gaagattaac atcaaaaccg atggtaaagc taatgcaaaa ggtaatgccg
     2581 cccctaaaaa aggtccggta caagaaatag ccatcaaaaa ggcaaaaata cctccaaaaa
     2641 atgaaccact gtaaccacct ttggaggttg ctttatttgc tgtattcata cccggaacag
     2701 ttatctcaaa aacacctaaa agcgacaaag caaatacaaa cacaattgcc atcagcgtta
     2761 ccacaaaacc gggattctga ttttgtaaac cccagcctac cgattcaccc gctttttgta
     2821 aagcgataaa aattactgcc ataatagtaa atgagattaa caccccaaaa gtgtaaacca
     2881 aaatatgatg caaaaccttg gttctatcct tctgtgcctg attcattatg ctcattatcc
     2941 ttatgggtaa aatgggtaac acgcaaggag tgatattcaa tataattcca cctaaaaagg
     3001 caaataggat atattttaag atttcgccaa gagacctttt ggcaataggt tctgtcgctg
     3061 ccctatcaac ttccgaaacg ggggaagttt ctcccttttc aatattttct gtggcttctg
     3121 tttcttcaga aacaactccc tcagcggctt cagcagtaat tatctgaaaa gaaagcttct
     3181 ttgttgcttc ttcgggggga tcacacatac cactttcata acaaaggtta tagcttaaca
     3241 aaacttcaac ctgtttgttg ccagcttttg ctgtctgctt cactgtaaaa ggtaaagtta
     3301 atgtaacctt gggatgataa ttccattctt catctgaaat gatattagtc ggtttggggt
     3361 attcaacctt accaaaagat aggtccggat gactggcttc taaatagaaa tattccggat
     3421 ttttaggatt tacagtctgc tttttgccct caggaattgt taaagtggct ttaataacgc
     3481 ctttttcgcc tggctttaaa acatcaggag aaagtaagaa tttgacagtt tgagcaaaaa
     3541 agggttgaat taacataatc aggcaaagtg ctaacaatag cttataacca cgattcatta
     3601 tcataatcct tttaacaatg attttttatt ataagttaat gcagaatttg ttccagacaa
     3661 attgttttat tataggatga gaaattatgg aatgtctcac gcagattaca cagatttcgc
     3721 agatggaata ggtatgggat gaacaggatt ttaaggattt agaggattga gttttagaaa
     3781 caaggatttt agaagaatat ttagccaccg aagccaccga aaatagttta cgcagaatag
     3841 attttcgaaa caaggatttt
//
LOCUS       DAOSTH010000118         3757 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_21984_length_3757_cov_14.030524, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000118 DAOSTH010000000
VERSION     DAOSTH010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21984_length_3757_cov_14.030524"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            713..3199
                     /locus_tag="PLB65_08065"
     CDS             713..3199
                     /locus_tag="PLB65_08065"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOQ78520.1"
                     /translation="MKKICFLLAFLIVISFLFAQFAEPKPDIINLISIVNADTLRAYV
                     QHLQNYQTRYALADNHLEVANWIKGKFESYGFTNTWMQEYPHNNTTQYNVIATIPGYL
                     HPDIYIIVGGHYDSISFNSDFFTFAPGADDNASGTAGVLEIARVMMATGYQPKCSIRF
                     IAFSAEEGAAWGSETYCDYAIAENQNIRVMINLDMIANNLEVSQEFQVMPYLGCEEYS
                     YETMRIAELYSSYQPVLGITGMGSDSDIFSRNGFNAVFFFERYISSYYHSNEDLIDYL
                     DFQYEAEIVKAATATVAVFANQPILVENLKVYDPGTGNSLLAEWDISSDPEVTKYAVY
                     YGTEIDSLTFWQYVNANQCSISGLEEGLFYNIAVCAVNDSGFESMRKYANGIPFLVPT
                     TPNLIADFPDKASITIKWLPNPELDIASYSIYRSLGLNGTKTLIATVPFSDTSYNDAN
                     VESNEEYYYYCITANDLEGNQSQFSTPLASRMVSLDRGIYVIDESKNFSGTSPFQPTD
                     EAVDDFYNDLLENFENVSHLDLEEQTSILKMADIGIYSSILWHGNELNDNTYPYEIRD
                     ALEQYIRSGGNLFITVYYPGKAFELNAGYPALFSADSFIYEVLGIGGVNYNGQARFKC
                     AIPNEDNYPALQVDSLKTLSTWNGHIFGVEGLEPVNPEESIYQYGSDYSSDSNQGTLN
                     GKSVGIHHTYAEGQTVCLSFPLYNMQAESAKKLIKYVFANLFQETTKPDIPPPNIGLK
                     VLPNYPNPFVNETTLTVENADPSKLMDVKIYNLKGQIVQSVFHGYPNKDLQFIWNGKD
                     NAGKVTSSGIYFVKVTSGKEKVITKIIKIH"
BASE COUNT         1185 a          640 c          710 g         1222 t
ORIGIN      
        1 aaaaatccaa aaagcggagc ttcagcgagc tccgactacc caaaagtgta gccggaggac
       61 gccggtcctc cgcaagaaaa aaaatccaag aatttattag cccattttcc tccgcactta
      121 aaaattgcag gttaatattt agtaatatcc tcatatattt acttgtcatc tgatattttc
      181 tacattaacg ccaactacat atctaatgca atcaccactc ttcgttctta agtgaaagcg
      241 ttaatcaagt gtttatcact ccttaatcaa gcgttaatta ttaacgcttg attaaggtat
      301 gttaaacgat agattaatgg cttcgcacat gaaatataga aagaaatacg gagattctat
      361 taggaaagtg gtagggaaag ccagcataat atggttccga tagctaaatt tattcgatat
      421 ttctaattta gtgctttcag ttgtagttga tagttctatg tatcttatag gaatgataac
      481 ttaaggggag atacttttta cctttaccac tcccaaaatg gtatttattt taattgacaa
      541 ttccacattc atatctttta ttagttttag tggagaaaag ttacggaaag tgaataagat
      601 aatgcaaaag aaattactcc tgtgaatggc actcatcaga caagaagcag cgggcagaat
      661 aagaaaaaac aattgcttag ctgcccgata aatatatttt ataggagaaa caatgaaaaa
      721 gatctgcttt ctactggctt ttttaatagt aatcagtttt ctgttcgctc agtttgcgga
      781 accgaaaccc gatattataa atttgatatc tatagtgaat gctgatacat tgagagcgta
      841 tgttcaacac ctgcagaatt atcaaacgcg ttatgctttg gctgataatc atttagaagt
      901 ggcaaattgg ataaaaggaa aatttgaaag ttatggcttt accaatactt ggatgcagga
      961 atatccacat aataatacta ctcaatataa tgtaattgcc actattccag gttacttaca
     1021 tccagatatt tatatcattg taggagggca ttatgattca atttccttta attctgattt
     1081 ttttactttt gctcccggtg cagatgataa tgctagtggc acagctggag tattagaaat
     1141 tgccagagtg atgatggcta caggatatca accaaaatgt tcaattcgtt ttattgcctt
     1201 tagtgcagaa gaaggtgccg cctggggatc ggaaacatat tgcgattatg ctatcgctga
     1261 aaaccaaaat atcagagtga tgataaacct ggatatgata gctaataatt tggaggttag
     1321 tcaagagttt caggttatgc catatttagg ttgtgaggaa tatagttatg aaacgatgag
     1381 aatagcagaa ttatatagtt cctatcaacc ggttttggga ataacaggta tgggctcgga
     1441 tagcgatatc ttttcaagaa acggatttaa tgctgtattt ttctttgaac gatatatcag
     1501 ttcctattat catagcaacg aggatttaat tgattatctg gattttcagt atgaagctga
     1561 aatagttaaa gcagctactg caaccgttgc tgtatttgcc aatcagccaa tattggtgga
     1621 aaatttaaag gtctatgatc ctggaacagg aaactcctta ttggctgaat gggatatttc
     1681 ctccgatccg gaagttacaa aatacgcggt atattacgga acagaaattg attctctaac
     1741 tttctggcaa tatgtaaatg ctaatcaatg ttctattagt ggacttgagg aaggactatt
     1801 ttataacata gctgtttgtg ccgtaaatga ttctggcttt gaaagtatgc gtaaatatgc
     1861 aaatggaatt ccttttttgg ttcctaccac accaaattta attgctgatt tcccggataa
     1921 agcatctata actatcaaat ggctgcctaa tccggaactg gatattgcca gctatagtat
     1981 ctatcgctct ctcggcttaa acggaacaaa aacactgatt gcaactgttc ctttctctga
     2041 tacttcttat aacgatgcga atgtggaaag taatgaggaa tattattact attgcattac
     2101 tgccaatgat ttagagggaa atcaaagtca attttctact cctttggcaa gtagaatggt
     2161 ttcactggac agaggtattt atgtgataga cgaaagcaaa aattttagcg gaacatctcc
     2221 ttttcaaccg actgatgaag cagttgatga tttttacaat gatctattgg agaactttga
     2281 aaatgtctcc catttggatt tggaagaaca aaccagtatc ttgaaaatgg cagatatcgg
     2341 tatctattca tctattttgt ggcatggcaa tgaactgaat gataacacat atccctacga
     2401 gattcgtgat gctttagaac aatatatccg gtcgggaggt aatttattta ttactgtcta
     2461 ttatccgggt aaggcatttg aattgaatgc tggctatcca gcattatttt ctgcagatag
     2521 ttttatctat gaagttttag gaattggggg tgtaaattat aatggacaag cacgctttaa
     2581 atgtgcaatt cctaatgagg ataattatcc tgctcttcaa gttgatagct taaagacact
     2641 gtccacttgg aatggtcata tctttggtgt ggaaggattg gaaccggtaa atcctgagga
     2701 aagtatttat cagtatggtt ccgattacag ttctgatagc aatcagggga cattaaatgg
     2761 taaaagtgtt ggtattcatc atacctatgc agaaggtcaa acagtatgtt taagttttcc
     2821 tctttacaat atgcaagcag aatcggctaa gaaattaatc aaatatgtgt ttgctaacct
     2881 tttccaggaa acaaccaaac ccgatattcc ccctcctaat ataggactaa aggtacttcc
     2941 caattatcct aatcccttcg tgaatgaaac gacattaact gtagaaaatg ctgatccgag
     3001 taaattaatg gatgttaaaa tctacaatct gaaaggacaa atcgtgcaaa gtgtatttca
     3061 tggctatcct aataaagatc ttcaatttat ctggaacggt aaagataatg caggaaaagt
     3121 caccagctcc ggaatctatt tcgttaaagt aacctcaggc aaggaaaaag taattaccaa
     3181 aataatcaag atacactgat tcttgccttc ccaaattgat taagttaatt gagattttag
     3241 ggccctatta tgttctttcc tctttaaaaa aatcctaaaa tccttgattc tataatcaaa
     3301 tctgtgtaat ctatgtaatc tatgagagat atttttcagg aagaacgaaa tcctcgtcgt
     3361 tctcctgaca gccgagttat gtaattccca ctatatctta cgactgcttg atagtttgtt
     3421 tgttgtgggt tgaccaggaa gttaacctcc ctatattttt acactggatt aaaatccagt
     3481 gttagtatgt gtcgtcccgc tgggactttt atacccattc tttattgcgc cgggttaaaa
     3541 cccgtcgtta atatgtatcg ttcctaacgg aacttttgtc gtaattctat ttttttgagg
     3601 ggcttatgcc gccgtcgcta taattgtatc gccctttgag ttaaaatcgg ttttgtttgt
     3661 gatcttccat ctaatttcat tcttttcccc tatctttgaa taaggtcagt aataatctta
     3721 tagaaaaggc cgattttgac ttaaagccga cagccaa
//
LOCUS       DAOSTH010000119         3731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_22173_length_3731_cov_12.470348, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000119 DAOSTH010000000
VERSION     DAOSTH010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22173_length_3731_cov_12.470348"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1576
                     /locus_tag="PLB65_08070"
     CDS             194..1576
                     /locus_tag="PLB65_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOQ78521.1"
                     /translation="MSKIYKKQKDSRLRTSFRTSITEGIFAQIYGNLSVIGSSFIVKL
                     MVILNASPMHYSLLSAIGQVSAIWQPLGVAITHKLRERKNACIFITALGRFLTFFLGL
                     SLLFHSQLQGIWFVLILLFFSAGLQSLGSNIWIAWISDLIPLSIRGRFFSKRNQVLIG
                     VGLIVSYIVSFHIDLFEAAKGAFKASYIHLLKAEHFFIPQNQPWFLAGVFAFASIIGL
                     IGLIFLKRQPENLITSVPELSLRERYLSPFRDKNFRLLLIFGIWWMLATGVGSPFWGP
                     YMMKKLSMSLFEIQLYNTISVCSSLLSYQFWGRFIDNNGNKTAMKICVALGGLNPMLW
                     LFTSPGNYTILWFEALISGCMWAGTGIITTNFVLSVAAKGSEQVYSGVYGAITGLAMM
                     VSTLACGIFFPSALTFGKRVFEPEQVIFGVGGILRWLSFIPLFFVVEKQNVPLHKALI
                     SLFKSPFEKR"
     gene            complement(1584..>2286)
                     /locus_tag="PLB65_08075"
     CDS             complement(1584..>2286)
                     /locus_tag="PLB65_08075"
                     /inference="COORDINATES: protein motif:HMM:NF017927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HOQ78522.1"
                     /translation="LDLSCLPKNNKKKKSSEVIMLTYTEIRSNAREFLRGKWNNPCAL
                     IILILALINFGVSCVPYLGSVISILVTGPFFLGLAIVFLKLVRGEEITIELMFEGFKD
                     FTRSFIAGVLIIVYIFLWSLLLIIPGIIASYAYSMTFFILADNPNLTANEAITASKEL
                     MKGHKTELFLLDLSFIGWILLGILSCGIGFLWIASYIETARAIFYHEIKGEAIVEPIV
                     DATFEEVANKPLEEE"
     assembly_gap    2287..2386
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2472..3416
                     /locus_tag="PLB65_08080"
     CDS             2472..3416
                     /locus_tag="PLB65_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOQ78523.1"
                     /translation="MAYLYVVLIFAILVLTFISRGLIIVRQASVVIVERLGKYYRTLD
                     SGIHIIIPIFDKTRPIHWRYNKIDYRGNVVVVNKIEDRIDLRENVYDFPRQNVITSDN
                     VSININALLYFQITDPYKAVYEIGNLPEAIEKLTQTSLRNVIGELTLQDTLTSRDAIN
                     AKLRDILDEATDKWGVKVNRVEMQEILPPEEIRTAMEKEMRAERDKRAKILQADGERE
                     YQIRVADGEKQARIARAEGESQAKKLVAEGERQAIISIAEAVKNTGTDPAQFQIALRY
                     IEAFREIVKQGDKTVVLPYESSALLGSVKTLGEIFQKV"
BASE COUNT         1138 a          627 c          687 g         1179 t
ORIGIN      
        1 aatctgcgta atctgcgtga acatatcctt aaatccttaa atccttgttt ctaaaactaa
       61 atctgcgtga tctgcgtaat ctgcgtgaac caatcctaaa atccttgttt cgaaaattca
      121 cttttggtta caattttctg gacaggaaca gccccttaat aaaatgagca atagaaatta
      181 taagggaatt taaatgtcca agatatataa aaagcagaag gatagccgtc ttcgaaccag
      241 ttttaggacc tctatcacag aaggtatttt tgctcagatt tatggtaatc tgtcagtgat
      301 aggttcttct tttattgtaa aattgatggt tattttaaat gcttcgccaa tgcattacag
      361 tttactaagt gcaattggtc aggtttctgc aatttggcaa cctttgggag tggctattac
      421 tcataaattg agggagcgta aaaatgcctg tatattcatt actgccctag ggcgtttttt
      481 aacctttttt ttagggctat cattattatt tcatagtcag ttacagggta tttggtttgt
      541 actgatattg ctttttttca gtgctggact acaatctctg ggatccaata tttggattgc
      601 ttggatcagt gatctaattc ctctatctat tcgcgggcgt ttcttttcca aacggaatca
      661 ggtcttaatt ggtgtagggc ttatagttag ctacattgtt agttttcata tagatttatt
      721 tgaagcggca aaaggtgctt ttaaagctag ctacatacat ttattaaaag cagagcattt
      781 ttttattccg caaaatcaac cctggttttt agctggtgtt tttgctttcg cttctattat
      841 tggtttaatt gggttaatct ttctaaagcg tcaacctgaa aacctgataa cttcggttcc
      901 ggaactaagt ttacgggaac gttatctttc accttttagg gataaaaatt ttcgtttatt
      961 actcattttt gggatatggt ggatgctggc aacgggagta ggaagtcctt tttggggacc
     1021 ctatatgatg aaaaagctat ctatgagctt atttgaaatt cagctatata acactattag
     1081 tgtatgttct tcccttttat catatcaatt ttggggacgc tttatcgaca ataacggcaa
     1141 caaaacagct atgaaaattt gcgttgcttt aggtggatta aatccaatgc tttggctatt
     1201 taccagtcct ggcaactata caattttatg gtttgaagca cttatttcag gttgtatgtg
     1261 ggctggaaca ggaataatca ctaccaattt tgttttatcg gttgcagcca agggtagcga
     1321 acaagtttat tctggagttt acggagcaat aacaggttta gcaatgatgg tttctaccct
     1381 tgcctgtggt atattttttc catcggcttt aacttttggc aaacgcgttt ttgaacctga
     1441 acaggtgatt tttggggtag ggggaatatt acgttggcta agttttattc ctcttttttt
     1501 tgtagtggaa aagcaaaatg tacctttaca taaagcgtta atatcgcttt ttaaatcacc
     1561 ttttgagaag cgctgaattt aaattattct tcctccagcg gtttattggc tacctcttca
     1621 aaagtagcat caacaatcgg ttccacaatt gcttcgcctt taatttcatg atagaaaatt
     1681 gccctagctg tttcaatgta acttgctatc cacaaaaaac caattccgca agataatatt
     1741 cccagtaaaa tccaaccgat aaaagaaaga tccagcaaaa agagctccgt tttatggcct
     1801 ttcatcaatt ctttactagc tgtaattgct tcattggcag ttaaattagg attatcagcc
     1861 agaatgaaaa aagtcattga ataggcatac gaagcaatga ttccagggat tatcaacaat
     1921 aaactccata aaaagatata aactatgatt aaaacaccgg caataaaact acgggtaaaa
     1981 tccttaaagc cctcaaacat caattctatt gtaatctctt cacctctaac cagtttcaga
     2041 aaaacaatag ctaatcccaa aaaaaaaggt cctgtaacaa gaatagaaat aacagaacca
     2101 agataaggta cacaagaaac tccaaaatta atcagggcta aaataagaat aatcaatgca
     2161 cagggattat tccatttccc tcgtaaaaat tcacgcgcat tactacgaat ttcagtatag
     2221 gttaacataa ttacctcact tgatttcttt tttttattat ttttgggaag gcaacttaag
     2281 tcaagtnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnncact ttgacctact
     2401 ctttttcagt tttttcttgc caaaaaaaca aagtggggaa ataaggaaaa aaaatagtaa
     2461 gggaggtaat aatggcttac ctatatgtgg tactcatttt tgcaatctta gttcttactt
     2521 tcatttctcg tggactgata attgtgcgtc aggcgagtgt cgtaattgta gaacgcttag
     2581 gaaaatatta tcggactttg gattccggga ttcacattat cattcctatt tttgataaaa
     2641 cccgtccaat tcattggcgt tataacaaaa ttgactatcg cggaaatgtt gttgttgtaa
     2701 ataaaataga ggatagaatt gacctaaggg agaatgttta tgattttccc cggcaaaatg
     2761 ttatcaccag tgataatgtt tccatcaata ttaatgcttt gctttatttt caaataaccg
     2821 atccttataa ggctgtttat gaaataggca atttacctga agcgattgaa aaacttactc
     2881 agacctcact gcgtaatgtt atcggagaat taaccttgca agatacttta acttcgcgcg
     2941 atgctattaa tgctaaattg cgtgatattt tagatgaagc taccgataaa tggggcgtaa
     3001 aagtaaaccg ggtggaaatg caagaaattt tacctccaga agaaattaga accgctatgg
     3061 aaaaagaaat gcgcgctgaa cgcgataaac gcgccaaaat attacaagca gatggggaac
     3121 gagaatatca aattcgggtt gcagacggag aaaaacaagc tcgcatagcg cgtgcagagg
     3181 gtgaatcaca agcaaaaaaa ctggttgccg aaggtgaaag acaagcaatt atatccatag
     3241 ctgaagctgt aaaaaataca ggaaccgatc cagctcagtt tcagattgct cttagatata
     3301 ttgaagcatt cagagagatt gtcaaacaag gagataaaac agtcgtttta ccctatgaat
     3361 catcagccct gttaggcagc gtgaaaaccc tgggtgagat ttttcaaaag gtataaataa
     3421 ctattttcta attacaagtt attaagtctt accctaggta acagaaaatt tacgctttat
     3481 cgtaaccgtg aagcggatct tggttacgcc aatatttttc cgaaggctat aaggaagata
     3541 tagtaaattc taatcctaat attgcaatag ccatataacg atattgcttc tttaatgtta
     3601 ggagtatttc tatatcttcc ttagggatct atggtagagc tgtggtgcag cctcttgggg
     3661 attttcccca gaagcaggac ggtagttcta cttcaggtaa ctaaggaagg agtatttttg
     3721 ttttgaagtt a
//
LOCUS       DAOSTH010000120         3699 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_22433_length_3699_cov_12.752470, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000120 DAOSTH010000000
VERSION     DAOSTH010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22433_length_3699_cov_12.752470"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(61..3213)
                     /locus_tag="PLB65_08085"
     CDS             complement(61..3213)
                     /locus_tag="PLB65_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor J domain-containing protein"
                     /protein_id="HOQ78524.1"
                     /translation="MNRKVLFLFLLFLIGIFFLCWGEQITIGNPASATYGLIPINRHH
                     NYSTYEMLYTSGELRSECLINKIAFYKRRGNPSLLISNVSIYMKVSSATSLSEGEISP
                     PYSEYQLVYSGNFPNNFGSSGWAEVTLFDPYPYNATGNLHILVVKGFEVWSTDRPDYT
                     YSSTGSTYLCRGSYSDNFQPTYLTPTYNRPVIRFDAEFLIENEPPLPPINPFPANSAI
                     DVLKNITLHWRSGGGCPSRYDVYFGDTSNPVKVISLLNETNFNPGILEYGKTYYWKID
                     PYNIYGYASDKYELEVWSFTVMNDPTITRNDLPYLQTFDNEWSGSPPSPLGWIVINAN
                     NDDYTWRQTNECNIPAYSEPYAAQGMGNTDDYLISPPLDLTAVNIKIKWYDMVESAQY
                     PNSYKVLLSTTDSDISSFTEELAVFTCYNTEWTEHSLDLSTYRDHHIYLAFYQYASTA
                     TNWSFGIDDFQIEKLPDVPILNYSPTEIIFPFTPVNTPSSYLNVTIANTGEGIIDLTE
                     DNFTLLGDYDDFEVDYSNLDAHLETDQSVNIPVRFYPRSFGEKNAILRINYSSRTQYD
                     VILKGTTYFEGLLMEDFEDDFLPVGWTKYVNNNNDIIQSNDNNHTFNGQFSARFSSLE
                     VSNDYNQYLFSPPVLINAFHHNLSFWHLKQNSEETLEWGIATDTNPENYDWKPVNLSC
                     VSWQKTQMDLKDYADQMVYLGFHYYGNNSQSVYLDDVHISCDIPANYPTEIDGITITS
                     TIHLNINNAVTATHPLVKALPNYNNLNAPIVLGLLGSGKGNLSLKLSSGNWYCLIYHS
                     GQWHYGSSYPCNITEGNTGIVNISNVEFGAKNDVILVIEEGIDPTLPVNLSRFTAVVT
                     SENFVIISWTAESETNHSGYNILRSEDKDLQNAIMINGSIINKGSSEGTQIKYNYTDF
                     EVYTNMIYYYWLENVSLDGNSEFYGPITVIIGDPTQEPVPPTVPMVTKLYNAFPNPFN
                     PNTNIRYSLKEAGKVKIEIYNMKGQKIKTYNLEHTSPGYYQVSWDSCDEKGRSVASGI
                     YLYRLSTGNYTSAKKMVLAK"
BASE COUNT         1246 a          675 c          621 g         1157 t
ORIGIN      
        1 taaaccttat aaaccctttc aaccttctaa acctttctta aattcatttt acattctaca
       61 ttatttagct aagaccatct ttttagcgga agtataatta ccggtggata aacgatagag
      121 ataaattcca ctggcaacgc ttcttccctt ttcatcacag ctatcccagg aaacctgata
      181 gtaaccagga cttgtgtgtt ccagattgta ggttttaatc ttttgtccct tcatattgta
      241 tatttcaatt ttaacttttc cagcttcttt cagggaatag cgaatattgg tattcggatt
      301 aaaaggattt gggaatgcat tatacaattt tgtaaccatc ggaacagttg gaggaaccgg
      361 ttcttgagtt ggatcgccaa taataactgt tataggaccg taaaattcac tgtttccatc
      421 aagggaaacg ttttccagcc aataataata aatcatattc gtatacacct caaaatccgt
      481 gtaattatat ttaatctgag ttccttcaga agaaccttta ttgattatag aaccgttgat
      541 cataatagca ttttgtaaat ctttatcttc actacgcaaa atattatagc cggaatgatt
      601 agtttcactt tctgcagtcc aggaaataat aacaaaattt tctgaggtta ctactgccgt
      661 aaaacgggat aggtttaccg gcaaagtagg atcaatgcct tcttcaatta ctaaaataac
      721 atcattctta gctccaaatt ccacattaga tatatttact atgccggtat tcccttcggt
      781 tatattgcag ggataagaac ttccataatg ccactgtcca ctatggtaaa ttaggcaata
      841 ccaatttccg gaactgagtt ttagagataa attccctttt ccagaaccta ataagccaag
      901 cacaatagga gcgtttagat tattataatt cggtaatgcc ttcaccaatg gatgagtagc
      961 agttacagca ttattaatat ttaggtggat agtggaagtt atggttattc catctatttc
     1021 tgtaggataa ttagctggaa tatcacatga gatatgaaca tcatcaagat aaactgactg
     1081 agaattattt ccataataat gaaatcccaa atagaccatt tgatccgcat aatctttcaa
     1141 gtccatttgg gtcttttgcc aggaaacaca gctgagatta acgggtttcc agtcataatt
     1201 ttcaggattg gtatccgttg caatacccca ttccaaggtc tcttcactat tttgtttaag
     1261 atgccagaac gaaagattat ggtgaaaagc attgattaaa acaggaggac taaacaagta
     1321 ttggttataa tcgtttgaaa cctcaagaga actaaatcgg gctgaaaatt gtccattaaa
     1381 tgtgtggtta ttatcattgc tttgaataat atcgttatta ttattaacat attttgtcca
     1441 tcccaccggc agaaagtcat cttcaaaatc ctccataaga agaccctcaa agtatgtagt
     1501 tcctttaaga attacatcat actgagttcg agaactataa ttaattcgca aaatagcatt
     1561 tttttctcca aaggagcgag gatagaaacg aaccggaata ttaacacttt gatctgtttc
     1621 caaatgtgca tcaagattgg aataatcaac ctcaaaatca tcatagtcgc ctaaaagcgt
     1681 aaaattatcc tccgtaaggt ctataattcc ctctcccgta ttggcaatgg taacatttaa
     1741 atagctggaa ggagtattaa caggggtaaa gggaaaaata atttctgtag gtgaataatt
     1801 cagaattggc acatcaggca gcttttcaat ctggaaatca tcaattccga aactccaatt
     1861 tgttgctgtg gaagcatatt ggtaaaaagc aagataaata tgatggtctc tgtaagtgct
     1921 caaatccaag ctatgttccg tccattcagt attataacag gtaaaaacag ctaattcttc
     1981 cgtaaaagaa gagatatctg aatcggtagt agaaagcaaa actttatagg aattaggata
     2041 ttgggcactt tctaccatat cataccattt tatttttata ttaaccgctg ttaaatccaa
     2101 aggtggagaa ataagataat catcagtatt tcccatccct tgagcagcat agggttcgga
     2161 ataggcggga atattacatt cgttagtttg cctccaagta taatcatcat tattagcatt
     2221 tattactatc caacctaaag gagagggagg actacctgac cattcattat caaaggtttg
     2281 caaatagggt aaatcatttc gggttatggt tgggtcattc attaccgtaa atgaccatac
     2341 ttcaagctca tatttatctg aagcatagcc ataaatatta taaggatcaa tcttccagta
     2401 ataggtcttt ccatattcca gaatcccggg attaaaattt gtttcattca gaagggatat
     2461 aacttttacc ggattggatg tatctccaaa ataaacatca tagcgagaag gacaacctcc
     2521 tccactacgc caatgcagag ttatattttt taatacatca atagccgaat ttgccggaaa
     2581 aggattgatc ggaggaagag gaggttcatt ttcaattaaa aactccgcat caaaacgaat
     2641 tacaggccta ttataagtag gagttaaata ggtcggttga aagttatcag aataactgcc
     2701 ccggcagaga tatgttgatc ctgtagaaga ataggtataa tcgggcctat cagttgacca
     2761 tacctcaaaa ccttttacaa caagaatatg cagatttccc gtagcattat aaggataggg
     2821 atcaaaaaga gtaacctctg cccaacctga agagccaaaa ttgttcggaa aattgccact
     2881 gtaaacaagc tgatattcag agtagggtgg agagatttct ccttcagata aactggttgc
     2941 ggatgatacc ttcatataaa tgcttacatt ggaaattaat aatgaaggat ttccgcggcg
     3001 cttgtagaat gcaatcttat tgatcaaaca ctcagaccgt agttcccctg aagtatacaa
     3061 catttcgtaa gtggaataat tgtgatgcct gtttatggga attaagccat aagttgccga
     3121 agcaggattt ccaatcgtga tttgttctcc ccaacataag aagaaaatcc cgattaaaaa
     3181 taataggaac aaaaacaaaa ctttccgatt cataacttat gcctccttaa agatattttt
     3241 tcaaccaaaa aaagcaatat ttcctgttcg cttccagtgt tgaaattgta agaggaatca
     3301 aacaagaata cccaaaccaa tcaattttaa gctggatata atagcataaa ttgagcataa
     3361 gtaggaataa agaattttca ctggaaaagg aatcatttga agaaagaata ttatgaaagc
     3421 gaaaggcgtt atacttggaa atagatacta ctttaacatc cgtctttttt aaagtgtttt
     3481 ccgttgttgc taaaaaaacg ccttgcctta agctactatc cacattcctc tcctcaaaca
     3541 caaaaaaata ttttattttg attctatata tattttttcg cttttattaa tcacaccttc
     3601 agagctctaa cttatatcca aaaacttcta tattattctt tacttcattc tcattttcct
     3661 tttactttaa acacaaattc ctgtcaagaa aaatcttac
//
LOCUS       DAOSTH010000121         3646 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_22876_length_3646_cov_10.966862, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000121 DAOSTH010000000
VERSION     DAOSTH010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3646
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22876_length_3646_cov_10.966862"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1701)
                     /locus_tag="PLB65_08090"
     CDS             complement(<1..1701)
                     /locus_tag="PLB65_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78525.1"
                     /translation="MKKMIIITLLTVTFMGVLVAGITNYYNAPVASQGTYQEITGTSA
                     GAGGDTFLSPALDIGFTFNYCGTAYTQLKMASDGYLALGTSQTSSSLSNNLSTTSVLP
                     IIASLWDDLTSNTMSYTTIGTEPNRVFIAQWKNTKWNYNGTAGQNFQIRLYETSDKIE
                     LIYGSMITPNSPSASIGINCSPGGSGNFYSITPGNPITYSTTTSNNSISAITYLTSGT
                     TYTFTKVDPNAPPLPAVIVSPTNGQSNVSNLPTLSWSSGGGIVTKYLVYFGTDGGGTS
                     TPANILNGYDNLTSTSYTFANPLTYNTTYYWQIVPYNGVNPAANCPIWSFTTMDDPTI
                     YPPFTEGFENGNTHETAVLGWTQESVSGSNVWTANNSYTNYNRTPRSGNWNAFLQYSN
                     TRWMFKPIQLEADINYRVIIYARQDATSGASIKISYGATATAAGMTNAIISNTNITNG
                     EYQRLEGIFTPTTSGLYYIGILGTLTSTPWYLSIDDITIEEVPDNPVCSVYPESWDFG
                     TVVLGSSATKQFTISNTGGGKLMINSVETIGDYYSISSLPTDTELEAGENTNFTVKYE
                     P"
     gene            complement(2506..>3646)
                     /locus_tag="PLB65_08095"
     CDS             complement(2506..>3646)
                     /locus_tag="PLB65_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOQ78526.1"
                     /translation="SKIESQNKYCVTQQQSSFCLLQKILFHFFLTVRPLPNIRFFMQI
                     NDIIKIIFWMQILPCLAYCAYAKGNMYPLDSKYIKYEELNDNLDKIVQINPDLAQKRI
                     IGFSSAEYLPIYALQLGNKNASEAILIIGQHHGEEVIGVNIAFTLAETLLNGYSKNIK
                     QQHLLQRYQIWIVPTLNPEGFRIVSSGILKTKRKNNRDTDNNKKLDLRTDGVDLNRNY
                     PVFWDLDPETDTNSPFYKGSEPASENETKAIVELAQNHNFVLAIFLHSSISGAYNEHI
                     YLPARINNSELFQKNLALARTYADATKKDYLKGNYEVFEGSTSEVGNARNFFFHRMKT
                     PAFLVEIGGKNKRGQSIIHPPNKTLIKIVDKNVNALLSVFYKLDN"
BASE COUNT         1185 a          599 c          656 g         1206 t
ORIGIN      
        1 cggttcatat ttaactgtaa aattagtatt ttctcctgct tcaagttctg tatctgttgg
       61 caagctactt atactataat agtcacctat tgtttctaca ctatttatca tcaatttacc
      121 tcctccggta ttggaaatgg taaattgctt agtagctgat gaacccaaaa caacagttcc
      181 aaaatcccag gattcaggat atactgagca aacaggatta tcaggaacct cttcaatcgt
      241 aatatcatca atagaaagat accaaggtgt tgaagtaaga gtccctaata taccaatata
      301 gtataaacca cttgtagtag gggtaaatat accttccaaa cgttgatatt caccatttgt
      361 aatatttgta ttggatataa ttgcattagt cattcctgca gcagtagctg ttgctccata
      421 actgattttg atacttgcac cggaagtagc atcttgtctg gcataaatta ttactctata
      481 atttatatct gcttccaatt gaattggttt aaacatccat cttgtattgc tatattgtaa
      541 aaaagcattc caatttccag atctgggagt tctgttataa ttagtgtatg aattattagc
      601 agtccataca ttggaaccac ttacactttc ttgagtccat cccaatacag cagtttcatg
      661 tgtgttgcca ttttcaaagc cctctgtaaa tggaggataa attgtcggat catccatagt
      721 tgtgaaagac cagattggac aattagcagc aggattaaca ccattataag gaacaatttg
      781 ccaataataa gttgtattat atgttaaggg gttagcaaat gtataagatg tggatgtaag
      841 attatcataa ccattaagta tattggctgg agttgaagtt ccaccaccat ctgtaccaaa
      901 ataaactaaa tattttgtta caatgcctcc acctgaactc caactcagtg ttggaagatt
      961 agaaacatta ctttgtccat ttgtaggaga gacaataaca gcaggtaaag gaggagcatt
     1021 gggatcaacc tttgtgaaag tgtaagttgt cccggatgta agataagtta tagcactaat
     1081 tgagttattt gatgtagtag tggaatatgt tataggattt ccgggagtaa tactatagaa
     1141 atttccagag ccaccagggg aacagtttat tccaatagaa gcacttggag aattaggagt
     1201 aatcattgaa ccatagatta gctctatttt atcacttgtt tcatagagtc gaatttgaaa
     1261 attctgaccg gcagttccat tatagttcca ctttgtattc ttccactgtg ctataaaaac
     1321 tctatttggt tcagttccta tagtagtata agacatagta ttagaagtta gatcatccca
     1381 caaagaagct ataattggta aaacagatgt tgttgaaaga ttattactta aactagaaga
     1441 tgtttgcgat gtccccaaag ccaaataacc atctgaagcc atttttaact gtgtgtatgc
     1501 agttccacaa taattaaatg taaaaccaat atccaatgca ggagatagaa aagtatctcc
     1561 acctgctcca gctgaggttc cagttatctc ttggtatgtt ccttgagaag caactggggc
     1621 attataataa tttgtaatac ctgctactaa cacacccata aaagtaactg ttagtagtgt
     1681 aataattatc atttttttca taataaaaac tccttataaa atgaagattt ttttgatttt
     1741 tgtaaatgga aggaattatc ttcaatcgta tttgggtggg aagtttgtat taggactgaa
     1801 gatgtttcct gtttgttttt gaaggctaga aagtagggaa atacagcgtt tgatgtttct
     1861 gaatttctat aatataaaaa gtaataatgg agcgtgaata agagattacg gaaaaaatca
     1921 aacgagcaga ttttgcattt ttgaaaattg tgaaaataca gagaaacagc acttccccaa
     1981 taggatgggg agggttgtgt tttattatga aaagggaagg tttttattaa gaaattgggg
     2041 agagttatct tatctgcgcc ttggcaaaat gtggctcttt tttgtacaca agggttgggc
     2101 tttggctcaa aataaaaaaa caatatattc tccttagaat aaacaaatat tctgcttagc
     2161 ttctgttatt ccacaagaaa ggaacacagc ttccgaaaca gaattaagtt acgaaaatac
     2221 aaaccgttaa gatacttttc aagtaccttt tccattacaa tttaattaaa atggcagctg
     2281 agaattatgt caagtacaaa ttttgtcacc caatgatttg aataggtttg caaagagcag
     2341 aattatccca atttaaagga ttttaattcg ccattaggac actgcagagc attaaaccac
     2401 actatagatt acacatatct cacagacaaa ataggtttta gaaatatgtg ttattgatca
     2461 taatatgata ttgtatgaga tgttcataac caattgaact ataatctagt tatccaattt
     2521 gtaaaacaca ctcagcaagg catttacatt tttatccaca attttaatta gcgttttatt
     2581 aggtgggtgg ataatacttt gtccgcgttt attcttcccg ccaatttcta ctaaaaaagc
     2641 tggtgttttc attctatgga agaagaaatt gcgcgcattt ccaacttccg aagtggagcc
     2701 ctcaaaaact tcataatttc ctttcaggta atcctttttg gttgcatctg catatgtcct
     2761 tgccagggca agattttttt gaaatagttc agaattatta atacgagccg gcagataaat
     2821 atgttcatta tatgccccag aaattgagct atgcagaaag atagccagaa cgaaattatg
     2881 gttttgagcc aattccacta tagcttttgt ttcgttttca gaagcgggtt cggagccctt
     2941 atagaaggga ctattagtat ctgtttcagg gtctaaatcc caaaaaacag gatagttacg
     3001 atttaaatca acgccatcag tgcgtaagtc taatttttta ttattatcgg tatcgcgatt
     3061 atttttgcgt ttagttttta agattcccga gctcacaatc cgaaaaccct ctggattcaa
     3121 agttggaaca atccatattt gatagcgctg taaaagatgt tgttgtttta tatttttaga
     3181 atagccattt agcaaggttt ctgctaaagt aaaagcaata ttcactccga taacttcttc
     3241 cccatgatgt tgaccgatta tcaaaattgc ttctgaggca tttttattgc caagttgcaa
     3301 agcataaatt ggcaggtatt ctgcactgct aaaaccgatt atgcgttttt gggcaagatc
     3361 ggggtttatc tgaacgattt tatccagatt atcattcaat tcttcatatt taatatattt
     3421 ggaatcgagt ggatacatat tccctttagc gtaggcacaa tatgctaaac agggcaaaat
     3481 ctgcatccag aatatgatct ttataatatc gtttatttgc ataaaaaacc ttatattagg
     3541 taatggcctt accgtcaaga agaaatgaaa aaggattttt tgaagaaggc aaaaagagga
     3601 ctgctgttgt gtaacacaat acttgttttg actttcaatt ttgctt
//
LOCUS       DAOSTH010000122         3617 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_23117_length_3617_cov_12.813307, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000122 DAOSTH010000000
VERSION     DAOSTH010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23117_length_3617_cov_12.813307"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(319..2205)
                     /locus_tag="PLB65_08100"
     CDS             complement(319..2205)
                     /locus_tag="PLB65_08100"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOQ78527.1"
                     /translation="MKQLKIDLLLILVLVTITLGGTAKQDSLKTYTLPTVYVIVEKPS
                     EAIGSLHIIEGEDVKNALSLREAMQNSVGISATIGTKDESNLRLRGFRKNEIKVMVDG
                     KPLNNGYFGNVDISKLSLLDIEEIQILKGPASPLYGTNNMGGVVNIITKAPPNNYWLE
                     LNTLFKRNNTNEITLSSAHSFDTWNYRIGVSQEKTDGFVLSEKFIPTPYEDGGVRNNS
                     DKCLYNISGSLEKEFLNFHNLGVDFGYNKMDKKNIPSSIYEQRYRQYQDWMRYHIGLW
                     TKMQVSESGTLNLQLINDGSTDRYLEYKDPAYENLALDSKMHNTGFGFHSRFTKTFTG
                     NKKLDIGYNYDFQGNNRKDNDSYQQWTDSVVHIHQAFTQFECTIIPKLLLTSGIGVNT
                     RINRDNNKIQPQLEPAVGIVYEIIPDSETSFAMGRNTSHPTMRQLYSASKGNPDLKPQ
                     NSLKLEINHNQALWQNKISLSFSVYFNDTKDLIDLYNGRYENIYKVQSQGTEIGVSFS
                     PDKFFQTSVNYAYLSYRKISDYYLTETPKNSLELCQQFTFPYKIGFRINSSYRDRCLS
                     QDDDGRYHTLAPYWKHDLILTIPWKRLKLSIAVENVLDEDYQSEYGYPEPGRNFSLGL
                     QAKI"
     gene            complement(2227..3039)
                     /locus_tag="PLB65_08105"
     CDS             complement(2227..3039)
                     /locus_tag="PLB65_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78528.1"
                     /translation="MKKFSLLLLVFLTLLMFSCSEKDDNPTNVDFYGYKLDQFISNTI
                     LRDLIDPNASDTLNFRELFAYEIEASDGFSPRNSSYAGYDLTWNFFKEGYIVPADNNR
                     TWFPDELGLPNAFKVKSTTQFNLYRKVEVTSGESSKTIELKGLTIYTIPNWNSANEEA
                     IKLSDLLQGISLSDSSNIRLIAIDDYYKDYTPEQIAEGYYLLDSEVTTFPEINDTLPG
                     ALKKFKKLASIEVINGSSQDHNFANAPHDKKDIAIPVPANFSDYEATPLPDY"
     regulatory      complement(3082..3200)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3255..3458
                     /locus_tag="PLB65_08110"
     CDS             3255..3458
                     /locus_tag="PLB65_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78529.1"
                     /translation="MTQIKNENKYGMNRMIWAEGRGLRAEGLGQRENRYGMNRMIWAE
                     GRGLRAERKKVWDEQDKRDLQDF"
BASE COUNT         1107 a          673 c          703 g         1134 t
ORIGIN      
        1 gcttttggga accgggaaag gtaaataaaa gaaaaataat cggggcttac tttttacgag
       61 gggcttacac caccatcgct atcattgtgt cgccctccgg gctttaaggt atctcaaccc
      121 tctcaacctt ctaaaccttc tcaaccctct caaccctata aaccttctca accctctaaa
      181 ccatctcaac tttctcaacc ttctcaactc tataaacctt ctaaacccaa taaactttat
      241 aaaccctaca atcccttcag accttacaac cttcagaact taagacctct ggacttctcg
      301 ccttcttgat ctcccgacct atattttcgc ttgcaaacca agagaaaagt ttcttcccgg
      361 ttctggatat ccatattcgc tttggtaatc ttcatccaag acattttcca cagctataga
      421 aagtttaagt cgtttccagg gtatggttaa tattaaatcg tgtttccaat agggagctaa
      481 agtatgatat cttccgtcat cgtcttgaga aagacatctg tctctgtaag aactgtttat
      541 tctaaatccg attttatagg gaaaagtgaa ttgctggcat aattcaaggg aattttttgg
      601 tgtttcagtt aagtaataat cgcttatttt ccggtagctg agataagcat aattaacgct
      661 ggtctggaaa aatttatccg gagaaaagct tactccgatt tctgttcctt gtgattgaac
      721 tttatagata ttttcatagc gtccgttata caaatcaatc aggtctttgg tatcgttaaa
      781 ataaacgctg aaagacagag aaattttatt ttgccaaagt gcctgattat gattaatttc
      841 cagtttcaga gaattttgcg gttttaaatc gggatttccc ttactggctg aataaagctg
      901 acgcatagtt ggatgagaag tatttcgacc catagcaaaa gatgtttcac tatcaggaat
      961 tatttcatac acaattccta cggcgggttc caattgaggt tgaattttgt tattatctct
     1021 atttattctg gtattaactc ctattccgct ggttaataac aacttaggaa taatggtgca
     1081 ttcaaattga gtaaatgcct gatgaatatg gacaaccgaa tccgtccatt gttggtaaga
     1141 atcattatct ttccggttat tgccctggaa atcgtaatta taaccaatat ccagtttttt
     1201 atttcccgta aatgttttag taaaacggga atgaaagcca aaaccggtat tgtgcatttt
     1261 agaatccaaa gctaaatttt cataagcagg gtctttatat tccaaatagc gatcggtgct
     1321 tccatcattg attaactgca gatttaaagt gccggattca cttacttgca ttttagtcca
     1381 taagccgata tgataacgca tccagtcctg atattgccta tagcgttgtt cataaataga
     1441 agaaggaata ttctttttat ccattttatt atagccaaag tccacaccta aattatggaa
     1501 atttaagaat tccttttcca gagaaccgga aatgttatag aggcatttat cgctgttatt
     1561 acgaactcct ccatcttcat aaggggtagg aataaatttt tcggagagca caaaaccgtc
     1621 tgttttttcc tgggaaactc ctatacggta attccaggta tcaaaactat gagcggaaga
     1681 tagggttatt tcattggtat tgttgcgttt aaagagtgta ttcaattcca gccagtaatt
     1741 atttggcggg gctttggtaa taatatttac aactccaccc atattatttg ttccgtaaag
     1801 aggtgaagca ggacccttca aaatctgaat ttcttcaata tccagcaaag agagtttact
     1861 gatatccaca ttgccgaaat aaccgttatt taagggtttg ccatctacca tcaccttgat
     1921 ttcgtttttg cggaaaccac gtaagcgtaa attactttcg tcttttgtgc caatagtagc
     1981 gctgatgcca acgctatttt gcattgcttc gcgtaaagaa agcgcatttt ttacatcttc
     2041 accttcaatt atatgcagag aaccaattgc ctctgagggc ttttctacta ttacataaac
     2101 ggtgggaaga gtataggtct tcagcgaatc ctgttttgct gttccaccaa gagtaatggt
     2161 aactaatact aagattagta gcaggtcaat tttcaactgc ttcacttctt tggcgaaata
     2221 gtttttttaa tagtccggta aaggagtagc ttcataatcg ctgaaattgg caggaacagg
     2281 aatggctata tcttttttat cgtgaggcgc attggcaaaa ttatgatctt gtgagctgcc
     2341 atttatcact tctatgcttg ccaatttctt aaactttttt agcgctcctg gcaatgtatc
     2401 atttatctca ggaaaagttg taacttcaga atccagcaga taataaccct cagcaatttg
     2461 ttccggggta taatctttat aatagtcatc tatagcgatt aaacgaatat tactgctatc
     2521 ggataaactg ataccctgta aaagatcgct gagtttaatt gcttcttcat ttgcggaatt
     2581 ccaattcggg atagtataaa ttgttaaacc tttcaattca atggtcttac tgctttctcc
     2641 actggtaacc tcaactttgc ggtataaatt gaattgggtt gtgcttttta ctttaaaagc
     2701 attaggaagt ccaagttcat cgggaaacca ggtcctgtta ttgtcggcag ggacaatata
     2761 gccctcttta aagaagttcc aggttaaatc gtaaccggcg taagatgaat tacggggact
     2821 gaatccatcg ctcgcttcaa tttcataagc gaataattcc cggaaattca aggtatcact
     2881 tgcattggga tcaattaaat cgcgtaaaat agtgttggaa ataaattgat ccagtttgta
     2941 accgtagaaa tcaacatttg ttgggttatc atccttttca ctgcaggaaa acataagcag
     3001 cgtaaggaaa acgagtaaaa gtaagctaaa ttttttcatt tttcctccat aataagatat
     3061 agaggcagac agggatgaaa agttatattt ccactgttgc ctttccaaat acgggaggca
     3121 taatcgtttc caattatacc caatggcgca attccatacc aaaggggttt aggaatatgc
     3181 gctcggcttt tttcaaaaaa tccaaagcag gaaaatttgt caaccccaac ttaatgttca
     3241 cgcagatttc gcagatgacg cagattaaaa atgagaataa atatgggatg aacaggatga
     3301 tttgggcaga gggcagaggg cttagggcag agggcttagg gcagagagaa aataggtatg
     3361 ggatgaacag gatgatttgg gcagagggca gagggcttag ggcagagaga aaaaaggtat
     3421 gggatgaaca ggataaaagg gatttacaag atttctagta tcagttccgt aggaatgaaa
     3481 gctattatag aagggtttca acccggcgag taagaggaga atgaaaaaag ggctcacgca
     3541 gattttattt gtatttggca gagaggacta agaacagaaa aggacaaagg aagtatgata
     3601 tgcagaagta taactat
//
LOCUS       DAOSTH010000123         3416 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_24900_length_3416_cov_10.231181, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000123 DAOSTH010000000
VERSION     DAOSTH010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3416)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3416
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24900_length_3416_cov_10.231181"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            447..1784
                     /locus_tag="PLB65_08115"
     CDS             447..1784
                     /locus_tag="PLB65_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOQ78530.1"
                     /translation="MTKEYRFKGINPEGKLVQGTFMADSKKDAKAQLAKLELRYKLRI
                     QTFEKKRDWLYKVQVKGRKAVKGRLSAFSKEEVVAALSKMGYDKVQVNPVLFDLPSKP
                     SVADIMMFIKLSSTMLRDKMNFGKILEMLAEEQPNRGFRDALLQIESQLKSGAEGREV
                     FMRYSHIFGKFPAYMLGLATRSGNMAEVFDATSKFIERDMEIKKNIKKATISPAFALL
                     ATIGATIYYIIDIFPATAKMFLKYDLPLPPMTKATLEVSDWLGVYWWVILLIFLIPVI
                     IIWRWWSTPKGRIWRDQHIVKLPLIGNLLHKQSIEIYFRVFGTIYSGAGDNIETIKTA
                     AEACRNAWMEDRIKKVTIPLMLKEGEAFVPAMEQSGVFTRTALMRLKTGQETGTILQS
                     AQQIATFYEAETTYKMNNLIEYIQSIVGLVVAIAITFLTVVSAEIATISPPTY"
     gene            1798..>3416
                     /locus_tag="PLB65_08120"
     CDS             1798..>3416
                     /locus_tag="PLB65_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOQ78531.1"
                     /translation="MLNNKFSKYLIDNELFDEDTMEKAEKKMNEYGGTSSGALARVLE
                     IEFGAPHDKIYEALALHYAFPTFKHTIEEITEQQLEVCKNILSKLHRDTDEDFKKRLF
                     FNKILPFAIKRTPREVLQVLVSDPTNLLIQEIPVRTPYKRMEVYWAPVKLIEAIIEKV
                     APQQNEFLMMLEEAGQVLSNIEETEETVINEQELDEEINKSLLVNLFEGALIEAVRRD
                     ASDIHIIPSGKTSTDFYFRIDGKLQLWFRQENTAPEAVAAVVKDRSTGVDRFERDTAQ
                     DGFAQRYIDENLIRFRISILPIVSQEYERRFESIVIRVIDDRKVITDFRKLGFQEQAE
                     REFLKAINTSKGIVIVTGPTGSGKSTTLIAALHHVIHPGVNVLTCEDPVEYAIKGARQ
                     LKIGHKMDFEQAVRSILRHDPDIVMVGEIRDKITADTAIKLANTGHLTFSTLHTNDAP
                     SAITRLYKMGVETFLLAYSINIIIAQRLVRKLCPYCRKPLSKEHWDAAMQMGISKEEL
                     EAGKIYEPVGCPKCNGTGYKGRINVAEALYF"
BASE COUNT         1131 a          591 c          696 g          998 t
ORIGIN      
        1 aattttgaaa tctggatacc agttagaatt cttggacagg tgataatttg gctggctgat
       61 agtgaattct tgttttaact ctcaattttg aaatcttgat gccagttcaa agtaagatcc
      121 cctatcttga aatagtagga aggtggtttc ttaaaggcct tcgcaaaatt gaaagtcaaa
      181 acaagcaggt gtgttttgtt ttgactctaa accgattctt tattcaattg caacgaggtt
      241 tatatagcaa caggtaattt tgttcgtatc tctttcctga caaagctgaa gctcggttta
      301 gggttaaaat aaaacatcct cacggtgggg aaaaaacaag cacctctctc tttcccataa
      361 caacggtaaa aatagtaaaa aaagcgtgac taaaatataa acaacaacta tggtgttttt
      421 atattattat agccatagga gattagatga caaaagaata tcgcttcaaa ggtataaatc
      481 ctgaaggtaa gcttgttcaa ggcacattta tggcagatag taaaaaggac gcaaaagctc
      541 aacttgctaa attagaattg cgttacaagc ttaggattca gacctttgag aagaagcgag
      601 attggcttta caaggttcaa gtgaagggac ggaaagctgt taagggtcgt ttaagtgctt
      661 ttagtaaaga agaagtagta gctgccttaa gcaaaatggg ctatgataaa gttcaagtta
      721 atcctgtgtt atttgattta ccatccaagc cctccgttgc agatataatg atgtttatta
      781 agctatcatc tacaatgctt agagataaga tgaactttgg caaaatcttg gaaatgttag
      841 ctgaagaaca acccaatcgt ggttttcgtg atgctctttt gcagattgaa agtcagctaa
      901 aatcgggtgc agaagggcgt gaggtcttta tgcgctattc tcatatattt ggtaaatttc
      961 ctgcttatat gcttggttta gctacgcgaa gtggtaatat ggcagaagtt tttgatgcaa
     1021 ccagtaaatt tatagagcgc gatatggaga ttaagaaaaa catcaagaaa gcaacaatat
     1081 cacctgcttt tgctttgctg gctacaattg gtgctacaat ctattatata atagatatct
     1141 ttcccgctac agcgaagatg tttttaaaat acgaccttcc cttgcctcct atgactaaag
     1201 ctactttgga agtaagtgat tggttgggag tatattggtg ggttattctt ctgatatttt
     1261 taattccagt aataattatc tggcgttggt ggagcactcc caaaggtaga atatggcgcg
     1321 atcaacatat tgtaaagctt cctttaatag ggaacttgtt acataaacaa tccatagaga
     1381 tttattttcg ggtttttggc actatttata gtggtgcagg tgataatata gaaacaataa
     1441 aaacggctgc tgaagcctgt agaaatgctt ggatggagga tagaattaag aaagttacta
     1501 tccctttgat gttaaaagaa ggtgaagcct ttgttccagc tatggaacag tcaggtgttt
     1561 ttacccgcac ggctcttatg cgtttaaaaa ctggccaaga aactggaact atcttacaat
     1621 cggcacaaca aatagccact ttctatgaag ctgaaacaac ttataaaatg aataacttaa
     1681 ttgaatatat ccagagtata gttggtttag tagtagctat tgcaatcact tttttaactg
     1741 tcgtttcggc agaaattgct acaatttctc ctcccaccta ttagaaggaa tattacaatg
     1801 ctcaataaca aattttccaa atatttaatc gataatgaac ttttcgatga agatacgatg
     1861 gaaaaagctg aaaagaaaat gaatgaatat ggaggaactt catctggggc attagcgcgc
     1921 gttctggaaa ttgaatttgg agctcctcac gataaaattt atgaagctct tgcccttcat
     1981 tatgccttcc caaccttcaa acatacaatt gaagaaatta cagaacagca attggaagta
     2041 tgtaagaata tattaagcaa actccataga gacacagatg aagatttcaa aaaacgcttg
     2101 ttttttaata aaatccttcc ttttgcaata aaacgcactc ctcgggaagt gttacaggta
     2161 ttggtaagcg atcctaccaa tttactgatt caagaaatac ctgttcgcac tccctataaa
     2221 cggatggaag tatattgggc tccagtgaaa ttgatagaag ccataataga aaaggtcgct
     2281 ccccagcaaa atgaatttct tatgatgctg gaagaagcgg gtcaggtttt atcaaatatc
     2341 gaagaaaccg aagaaacagt tataaacgaa caggaactgg atgaagaaat aaataagagt
     2401 ttattggtga acctctttga aggtgccctt atagaagcag tgcgtagaga tgccagtgat
     2461 attcatatta tcccttccgg aaaaacctca accgatttct attttagaat tgatggaaag
     2521 ctgcaacttt ggttcagaca ggaaaatact gccccagaag cagttgcggc tgttgtaaaa
     2581 gatcgctcta caggcgttga ccgttttgaa agagatactg cccaagatgg ttttgctcaa
     2641 cggtatattg acgaaaattt aatccgcttt agaatttcta tcctgccgat tgtttctcaa
     2701 gaatacgaaa gacgattcga aagtatcgtt atccgggtaa ttgacgaccg gaaggtaatc
     2761 accgatttcc gtaaactcgg tttccaagaa caagcagaac gggaattctt gaaagccata
     2821 aatacttcaa agggaattgt gatcgttacg gggcccacag gcagtggaaa atctacaact
     2881 ttaattgctg cccttcatca tgttatccat cccggagtaa atgtgctaac ttgtgaagac
     2941 cccgtagaat atgcaattaa aggtgcacgc cagcttaaaa taggccacaa aatggatttt
     3001 gaacaagcag taaggtctat cctgcgtcac gatcccgata ttgttatggt tggcgaaata
     3061 agagataaaa taactgccga tacagcaatt aaacttgcta atacaggtca cttaactttc
     3121 agcacattac ataccaatga tgccccttcg gctattaccc gtttatataa aatgggagta
     3181 gaaacatttc ttctggcata ttcaatcaat attattattg cccaaaggtt agtgcgtaaa
     3241 ctttgcccat attgcagaaa acccctttcc aaggaacatt gggatgccgc tatgcaaatg
     3301 ggaataagca aagaagaatt ggaagcagga aaaatatatg agccggtagg ttgccccaaa
     3361 tgcaatggaa ctggctataa aggtagaata aatgtagctg aagcattata ttttta
//
LOCUS       DAOSTH010000124         3298 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_26126_length_3298_cov_25.002158, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000124 DAOSTH010000000
VERSION     DAOSTH010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3298
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26126_length_3298_cov_25.002158"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(251..703)
                     /locus_tag="PLB65_08125"
     CDS             complement(251..703)
                     /locus_tag="PLB65_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78532.1"
                     /translation="MKVKFKYGIRTYSGTLDELTFGSYFKDHVCIARRYVVPRLTDQN
                     NLIGGKMKNLAVIYGDVSESYKAELKQYAAMHSILTPKGKLPATAYALWVKMMFQFAS
                     ADAEHIDLLTLTYSDLQTVGDDILSIASAVSNGFMDNVPGADKLTANM"
     gene            complement(736..1173)
                     /locus_tag="PLB65_08130"
     CDS             complement(736..1173)
                     /locus_tag="PLB65_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78533.1"
                     /translation="MIVESKFELLFCGNCDGMVYYYNKRLGKMIARKWVKPKTTAANR
                     RLGAITRNLKSLKPSEGFIKDLRVYIALYDYSPGERHYMSWQNVYLTLMYALAKQNPA
                     VDLLTITREQITSDNLPCKSVKDAIEAGLLREVNGYENLTQEI"
     gene            1425..1952
                     /locus_tag="PLB65_08135"
     CDS             1425..1952
                     /locus_tag="PLB65_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78534.1"
                     /translation="MTKPVEPIQTMLQTYPNFKGSKVIDLLYAHPDTPIPAADMELAL
                     NLQIPPDFINRNRYRFAPIRMTDEQTLRCVDKRLNRLIELKAFNATTAYDDEIQALIR
                     YRKETTLPTGKIKCFNDDDSKAYDRLRKDIDTLLKQAEKDGYSEAVAIVKRCMRRGLS
                     FVWDSDYNYNLFTER"
     gene            2294..>3298
                     /locus_tag="PLB65_08140"
     CDS             2294..>3298
                     /locus_tag="PLB65_08140"
                     /inference="COORDINATES: protein motif:HMM:NF020811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HOQ78535.1"
                     /translation="MLRKISETQGELLTSPNYYDAEQPENKKKGHQAIVFVDLNDAWL
                     YYEQLGYKMVTFTLEEKKGKKVVSNMPQWKNSSLSVESIKQEHNAIAIITGEASQLMV
                     IDIDKRDVNIYSLLAEYGLEIDNYCYALTPSGGMHIYLNLSHSELWKKRYRCKTLTTT
                     NKNIGIDIRAEIALIFAPPSIITNGGCYEWVNMPVNKEDLDYDANKFIPIMDAIFGYN
                     NNPTPLAPIQKTITKSDSPTAGYYDIQEDYDQAAQLISKLNGIIINYNDWIRMGIALK
                     NRFGERGLSLWLLFAYNSAYQDTEEELIKKWNSFPITDNVHFGTFIYLTKKYLKNEIT
                     Y"
BASE COUNT         1041 a          623 c          595 g         1039 t
ORIGIN      
        1 acttggctaa gcaaaaagtg actaacttta taaatgcaat taacattcca tttatcactc
       61 tttatagagg aaaaactaaa tccctgtcat tcttcgggaa ttaagtaagg aattaatagc
      121 acctttttta acctttgcct gttagataat ttttcgggag cttatggcta atgactacca
      181 agaatatttt ataaaataaa tctgtgtaaa ataagcgaaa aaaaaggcgg gaactaagtc
      241 ccgcccttgt ttacatattt gccgtcagct tatccgctcc gggaacatta tccataaacc
      301 cgttggatac agcggaagca atagacagaa tgtcgtcacc tacggtttgc aaatccgaat
      361 aagtaagtgt taataggtca atatgttccg catccgcact ggcaaattgg aacatcattt
      421 ttacccacaa agcataagcc gtagccggaa gttttccctt gggtgttaaa atggaatgca
      481 tagcagcgta ttgtttcagt tctgctttgt agctttccga aacatctcca taaataacag
      541 ccaggttctt catcttgcca ccaatcaaat tgttttgatc tgtaagccga ggaactacat
      601 acctgcgtgc aatacacacg tggtctttga agtaggaacc aaaagttaac tcatccagag
      661 ttccactata ggttctaatg ccatacttga atttgacctt cataatagaa agccctcctt
      721 gtaatattta gtgttttata tttcctgcgt taaattctca tatccgttaa cctcacgcag
      781 aagccctgct tcaatagcgt ctttcacact tttgcagggc aggttatcag atgtaatttg
      841 ttctcgggta attgttaata aatcaactgc tggattttgc tttgccaacg catacatcag
      901 cgttaaatat acattttgcc agctcatata atgtctttca ccgggagaat aatcatagag
      961 tgcgatataa actcgcaggt ctttgataaa accttctgat ggcttcaggg acttcaaatt
     1021 tcgggtaatt gctcccaaac gccggtttgc tgccgttgtc ttaggtttca cccatttcct
     1081 ggcaatcatt ttgcctaagc gtttgttgta ataatatacc attccatcgc agttaccgca
     1141 gaagagtaat tcaaatttgg attctacaat cattatttta tcctcctata tatatacgcc
     1201 ggaaatttag cccctatttc tcaaatattt ttgtttcccc tggtactcac gcagatttta
     1261 ttttctcctt ctctcttttc ctctttataa aactctcctc tcccctcttc attcttccct
     1321 ctttttttct aataatttct cccctctttt ctcatatttt acttgacaag atattgttta
     1381 tctattattg gtattaggta tttagttact ataaggagat aaatatgact aagcccgttg
     1441 aacctatcca aacaatgctg cagacctatc ctaactttaa gggtagcaaa gtgatagacc
     1501 tgctttatgc ccatcctgat accccgattc cggctgcgga tatggaattg gctcttaact
     1561 tacaaattcc tcctgatttc ataaaccgca accgttaccg ttttgcccct attcgtatga
     1621 ctgatgaaca gaccttgcgc tgtgtagata aacgcctgaa ccgtcttatt gaacttaagg
     1681 cattcaatgc tacaacagct tatgacgacg aaattcaagc cctcattcgg tatcgcaagg
     1741 aaaccacttt gcctacaggt aaaataaaat gctttaatga tgacgattcc aaggcatacg
     1801 accgcctgcg caaagatata gacaccctgc tgaaacaagc ggaaaaggat ggctacagcg
     1861 aagcagtggc tattgttaag cgctgtatga gaagaggtct gagttttgtt tgggatagtg
     1921 actataatta taatttattt acagaaagat agatagagcg ttttaacttc tttaccagat
     1981 ttggctaata actctctaac tatcatataa ttaaaaaaat ggcgataaag atgaaaaata
     2041 ttacttgaca tactaacagc aatggttttt atggttacga taatatttat agagtaaatc
     2101 gtaaaagtga ggaaaaagaa tagaaaagaa taagatataa atttattatt ttctggatac
     2161 caatttcaca tttttatgcc tatatattaa agcaagtaaa ataactgtag gatttgctca
     2221 ttatcaaaaa gagctatttt cattaattgt ggaaatagtc aaacaaagaa aatcttaata
     2281 attaggagga tttatgttaa gaaaaattag tgaaacccaa ggggaacttc ttacttctcc
     2341 gaactactat gatgcagaac agcccgaaaa caagaagaaa ggacaccaag caattgtttt
     2401 cgtggatttg aatgatgctt ggctttatta cgaacaactg ggttataaaa tggttacatt
     2461 tactttggaa gagaaaaagg gtaagaaagt agtcagtaat atgcctcagt ggaaaaatag
     2521 ttctttatcg gtagagtcaa ttaaacagga acataatgcg atagccatca ttactggtga
     2581 ggcatcccag ctgatggtga ttgatattga taaaagagat gtgaatattt attctttact
     2641 cgcagaatac ggactggaaa ttgacaatta ttgttatgct cttacaccct ccgggggaat
     2701 gcatatttat cttaatttat cgcactcgga actgtggaaa aagcgttata ggtgtaaaac
     2761 gcttactact acaaacaaga atataggtat tgatatccgg gctgagattg cgctgatttt
     2821 tgctccccct tccatcataa cgaatggagg ttgttatgag tgggtaaata tgcctgtaaa
     2881 caaagaggat ttggattatg atgcgaataa gttcatccct attatggatg ctatatttgg
     2941 atataacaat aatcctacac ctttggcacc tatccaaaaa acaataacaa aatctgattc
     3001 ccctactgcc ggttattatg atattcagga agattacgac caagccgctc agttaattag
     3061 caaactgaac ggtattatta ttaattataa tgattggata aggatgggaa tagcgctgaa
     3121 gaatagattc ggggagaggg gtttatctct ttggctgctg tttgcctata attctgctta
     3181 tcaggatacg gaagaggaac tgataaagaa gtggaattcc tttcctataa ccgataatgt
     3241 tcatttcgga actttcatct atctaaccaa aaaatatctg aagaatgaaa ttacttat
//
LOCUS       DAOSTH010000125         3287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_26242_length_3287_cov_12.560953, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000125 DAOSTH010000000
VERSION     DAOSTH010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3287
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26242_length_3287_cov_12.560953"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3235
                     /locus_tag="PLB65_08145"
     CDS             <1..3235
                     /locus_tag="PLB65_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ78536.1"
                     /translation="CKPATFTQDCMGERYINNANGGGVAFIGNSDVGWSSEWTQLRDF
                     ANSIYETIGHPSSGRFDIGCAFQSAIGRSSSNWRLTLLGDPEMQIWTNTPQTLNVSVS
                     PTIVVSGTNTIAVTINNLPSGKTATICLQKGDEGYATRTVTSNSISYFTFTPHTSGQI
                     NVTVTGHDFKPYESTITVNTNQNQNIYISNLTFDDDKTGNSNGNSDQQIDAGETIELT
                     VELKNNGASVATNVSATLTCSSPYVSISNNNSSYGNISSGATKSSLSKYVFTIDKNAP
                     EILVNDLNPVKFILLITDGASHSYTDVFNIDVFASVIEQANKSIVTTTDGDKIIEPNE
                     TVTVNIDLFNKGKAQATGITGVLSGNSSYITSCSATPRNYPAIEKFGTKTNTSVYQFT
                     VSSSYTVGQALDFILQVENEFDKTWSFNFNLLDKPAKINTSTIDFTADKTEIELFWTP
                     YTNISGYNIYRSDADASGNPVGNYQKINTFAVPAAYFKDFGLNELTKYYYKISAVSLT
                     GNESELSDAFLAWTSYPSKGLFPIQMDIQDANSIISSINVSDINQDALQEIFTSITID
                     SKNSYLIGLNSDGTELFDIDGNVTSYSGFAYLNAAMNSTPAIGDLESNGQYKVLSMTR
                     DGSADRTNYFSCHSVKDNNSDGKPDLFWQHSISSPCYRAPIIANLDNSADGSLESVFY
                     TEWGGIRIYSATGQLLYSFGDGIGGTYGSLAVADIDRNGDKEIIGCYRNSSTGITGIY
                     IWHPNGTNYGANQPFYTLSGYNFGSSVVVCDIDNDGDKEILTSALKSGGTEGRILAIH
                     HNGTVVSGWNGNQKISYPNDWHSQDISVGDLNNDGNLEVVAIGSNIVKVWNKSGTQIS
                     STTVSSLNPGKLTPILADVDNDADIEIIFGSNDEGKIHALNMDGSKVLGFPLHIDDAL
                     QGSPCVADVDNNGNNEIIVGAGIKIYMWETNGNSQRIEWGSERHDCHNTGVYHEICPP
                     TIITSNTTWSTNKNLCGDIIINSGTLTIASTCTVTMSDYTRVLVNSGGELKVDAGKIM
                     NVSIKALSGSNIILKSNGYIKISDHGEFNICTGATFDYQYGTLDITQ"
BASE COUNT         1126 a          537 c          628 g          996 t
ORIGIN      
        1 ctgtaaacct gcaacattta ctcaggattg tatgggtgaa cgatatatta ataatgcaaa
       61 tggcggaggg gttgcgttta ttggaaattc agatgttggc tggtcatctg aatggaccca
      121 attgagagat tttgctaatt caatttatga aacaatagga catcctagtt ctgggagatt
      181 tgatataggt tgtgcatttc aatcagccat agggcgtagt tcttcaaatt ggcgactaac
      241 tcttttagga gaccctgaaa tgcagatttg gacaaatacc cctcaaacgc taaatgtctc
      301 tgttagtcct acaatagtgg tcagcggaac aaatacgatt gcagtaacaa ttaacaatct
      361 gccatcgggt aaaacagcaa caatatgtct gcaaaaaggc gatgaaggtt atgccacccg
      421 aacggtaaca tccaatagca ttagttattt tacctttacc cctcacacat caggacaaat
      481 aaatgttaca gtaacaggtc atgattttaa accttacgaa tcaacaatta cggttaatac
      541 aaaccaaaat caaaatattt atatttctaa cctgactttt gatgatgaca aaacaggaaa
      601 cagcaatggt aatagcgacc aacagataga tgcaggagaa accattgaac ttaccgttga
      661 attgaaaaat aatggagctt cagttgctac aaatgtttcc gcaactttaa cttgtagctc
      721 tccttatgtt tccatttcaa acaataattc aagttatgga aatattagtt caggagctac
      781 aaaatcgtca ttatcaaaat atgtttttac aatagacaaa aatgctcctg aaattctcgt
      841 aaatgattta aaccctgtca aatttatctt actgattact gacggggcaa gccattctta
      901 tactgacgtc tttaatattg atgtatttgc ctctgtaatt gagcaagcca ataaatcaat
      961 cgtcacaact accgacgggg ataaaataat agaacctaat gaaactgtaa ccgtaaacat
     1021 tgacttgttt aataagggca aagctcaagc aacaggtatt acaggtgtat tatcaggtaa
     1081 cagttcttat attacaagtt gttcggcaac acctaggaat taccccgcaa ttgaaaaatt
     1141 tggcactaaa accaatactt ctgtttacca atttacggtt tcatcttcct atactgttgg
     1201 tcaggctttg gattttatct tgcaggttga aaatgaattt gataaaacat ggtctttcaa
     1261 ctttaattta cttgataaac ctgctaaaat taatacttca acgatagact ttacggcaga
     1321 taaaactgaa atcgaattat tctggacacc ttacaccaac attagtggtt ataatattta
     1381 ccgtagcgat gccgatgcaa gtggaaatcc ggttggcaat taccagaaaa taaacacgtt
     1441 tgcagttccg gcagcttatt ttaaagattt tgggttaaat gagcttacca aatattacta
     1501 taaaatatca gcagtttcat taaccgggaa tgagagcgaa ctttcagatg cctttttagc
     1561 atggacttca tatccttcaa aaggattgtt tcctatacaa atggatatcc aagacgcaaa
     1621 tagtattata agttctatta atgtttcaga cattaatcaa gatgctttac aagaaatttt
     1681 tacgagtata acaattgata gtaaaaacag ttacctgatc ggattaaatt cagatggtac
     1741 tgaattattc gacatagatg gaaatgtaac ttcttatagt ggttttgctt atctaaatgc
     1801 ggcaatgaat tccacgcctg ctattgggga tttggaatca aatggacaat acaaagtact
     1861 ttcaatgaca agggacggta gtgccgatag gacaaattac ttttcgtgcc attctgtaaa
     1921 agacaacaat agtgatggta aacctgattt attttggcaa cattcaatat caagcccatg
     1981 ttaccgggcc ccgataatag caaacctgga taatagtgct gatggttctt tagagagtgt
     2041 tttttataca gaatggggcg gtataaggat atacagtgca acaggccaat tattatacag
     2101 ttttggagat gggattggag gtacttatgg ttcattggca gtggctgata ttgatagaaa
     2161 tggcgataaa gaaataattg gttgctatag aaatagttca acaggaataa caggtattta
     2221 tatttggcat cctaatggta caaattatgg tgctaatcaa ccattttata ctttgtccgg
     2281 ctataacttt ggaagttcag tagtagtgtg cgatatagat aatgatggag acaaggaaat
     2341 tttaacttct gctttaaaat caggtggtac agaaggaaga attcttgcca tacaccacaa
     2401 cggaacagtt gtcagcggat ggaatggcaa tcaaaaaatt agttatccca atgattggca
     2461 ttcacaggat atttcagtag gtgacctcaa taatgatggt aatcttgagg ttgttgcgat
     2521 aggctcaaat attgtaaaag tttggaacaa gtcaggtact caaatcagtt ctacaactgt
     2581 aagtagctta aatcctggta aattaacacc aatacttgct gatgtggata acgatgctga
     2641 tatagaaata atttttgggt ctaatgatga aggtaaaatc catgctttaa atatggatgg
     2701 ttcaaaagtt cttggtttcc cacttcatat tgatgatgca ttacaaggtt ccccatgtgt
     2761 ggcagatgtg gacaataatg gtaacaatga gataatcgtt ggtgctggca ttaaaatata
     2821 catgtgggaa acaaatggca attcacaacg tattgaatgg ggaagtgaaa ggcacgattg
     2881 tcataataca ggggtatatc atgaaatatg tcctccaacc ataattacct caaatacaac
     2941 atggagtacg aataaaaacc tttgtggcga tattattatt aattccggca cattaactat
     3001 tgcttccaca tgtacggtaa ctatgtctga ttacacgaga gttttagtaa attccggcgg
     3061 ggaattaaaa gttgatgctg gtaagataat gaatgtcagt attaaagctt taagcgggag
     3121 caacatcata ttaaaaagca atggttatat aaaaatcagt gaccacgggg aatttaatat
     3181 ctgcaccggg gctacttttg attatcaata cggcacatta gatattacac aataatttaa
     3241 aaaaaatagg aaatcatgaa aataaatatt tttattttaa tcatatt
//
LOCUS       DAOSTH010000126         3282 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_26296_length_3282_cov_14.339324, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000126 DAOSTH010000000
VERSION     DAOSTH010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26296_length_3282_cov_14.339324"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(72..1001)
                     /locus_tag="PLB65_08150"
     CDS             complement(72..1001)
                     /locus_tag="PLB65_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78537.1"
                     /translation="MSRYKEINLNKIKTYPIDQRYSKVNKNAFAQKGKVPVSDFLNCL
                     PDILAAKDIKELIATCKKAKQNHKPIIIGLGGHTIKCGLAPLLIEVMEAGFVSAFVCN
                     GSVAIHDYEIACFGKTSEDVSAALSDGSFGMAEETAKGINTAINKGFEEGLGLGEAIG
                     KELDNIAKNKELSLLASAYQFGIPVCVQVAIGTDIIHQSPYANGKAIGDCSMRDFRIF
                     AEKVSKLNEGGVFLNLGSAVIVPEVFLKALTVARNIYGKVQDFTTAVFDFNMHYRAKV
                     NVVQRPIENGGKGYYFIGHNEIMVALLLKAILE"
     gene            complement(973..1530)
                     /locus_tag="PLB65_08155"
     CDS             complement(973..1530)
                     /locus_tag="PLB65_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HOQ78538.1"
                     /translation="MAEKELLLHCCCAPCFIVPLYKLRAEGKKISAFWYNPNIHPLEE
                     YQKRRNTLRDFTFKENIELIEKDEYGLINFLQATLKDIENRCLYCYETRLNATAKIAK
                     ELGYTAFGTTLLYSKYQKHELIVDIALKMSAKYGVDFFYEDWRCLWWEGKKLAKETGL
                     YRQQYCGCIFSEEERYREQIQRDKP"
     gene            complement(1626..2771)
                     /locus_tag="PLB65_08160"
     CDS             complement(1626..2771)
                     /locus_tag="PLB65_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOQ78539.1"
                     /translation="MDYFLTEDQLEMQEIARRIAEEKIKPVSEKYDEEGIFPWDIIEV
                     MKQSDLFAILIPEEYGGISGKVVDLAVVTEELCTVDAGISLAFGATGLGMYPILIAGS
                     EEQKQKYLSQIAAGEKLAAFALTEANAGSDAGAIETTAKKEGDYYILNGTKQWITNGG
                     EAGIYCVFAMTDRTKGARGCSCLLVEKGTPGFNFGKKENKMGIRASATRELIFEDCKV
                     PAENLIGREGTGFITAMKVFDKSRPMVGAQAVGVARGAFEAAIKYSKERQQFGKPISS
                     FQGIQFMLADMATQIEAARALVMQTARMIDSGARNYSKESAMCKYYASDVAMKVTTDA
                     VQILGGYGYMKEYPVEKMMRDAKILQIYEGTNQIQRNIVAANLLKEF"
     gene            2983..3147
                     /locus_tag="PLB65_08165"
     CDS             2983..3147
                     /locus_tag="PLB65_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78540.1"
                     /translation="MQEFKVREFKVQEFKVREFKVQEFLVQSAGVQSAGVQSAEVQSA
                     GVLSSKFFFR"
BASE COUNT          975 a          728 c          511 g         1068 t
ORIGIN      
        1 aaaatcctta aatccttgtt tctaaaacta aatctgtgta atctgtgtaa tctgtgagag
       61 attataatca cctattccag aatcgctttc agcagcaaag ccaccattat ttcattatgc
      121 ccaataaaat aatatccctt tcccccattt tctattggac gctgcaccac atttactttt
      181 gctcgatagt gcatattaaa atcgaaaacc gcagttgtga aatcttggac tttaccataa
      241 atatttctgg caaccgtcaa tgcctttaaa aacacttcgg ggacaataac tgccgagcct
      301 aaatttaaaa acactccacc ctcattcagt ttagagacct tttccgcaaa aatcctaaaa
      361 tcccgcatcg agcaatcacc aatagcttta ccgttggcat aaggactttg atgaattata
      421 tctgttccaa tagccacttg cacgcaaacc gggataccaa attggtaagc agaagccagt
      481 aatgataatt ctttattttt ggctatatta tctaattctt tcccaattgc ctctcccaaa
      541 cctaatcctt cctcaaaacc cttatttatg gcagtattaa ttccctttgc agtttcttct
      601 gccattccga atgaaccatc tgataaagca gcagatacat cttcagaggt tttaccaaag
      661 caggcaattt cataatcatg aatagcaaca gaaccattac aaacaaatgc ggacacaaaa
      721 cctgcttcca taacttcaat tagtaaagga gccaaaccgc atttaattgt atgacctccc
      781 aaaccgataa ttatgggttt atgattttgc tttgcttttt tacaagttgc tattagttct
      841 tttatatctt tagcagcaag gatatctggt aggcaattta aaaaatcgga aaccggcact
      901 ttcccttttt gggcaaaagc atttttattt actttactat agcgttggtc aataggatat
      961 gtttttattt tattaaggtt tatctctttg tatctgctca cgatagcgtt cctcttcact
     1021 aaatatgcaa ccacaatatt gttgacggta aagtccagtt tccttagcca attttttccc
     1081 ttcccaccat aagcatctcc aatcctcata gaaaaaatct acaccatatt tggcgctcat
     1141 ttttaaggca atatccacaa tcagctcgtg tttttgatat ttactataga gcaatgtagt
     1201 accaaaagca gtgtaaccaa gttccttagc tattttggca gtagcattta aacgagtctc
     1261 atagcaatat aaacagcgat tttctatgtc ttttagagtt gcctgtagaa aattaatcag
     1321 accgtattca tccttttcaa tcagttcaat attttctttg aatgtaaaat cccgcaaggt
     1381 gttgcgcctt ttttgatact cctcaagcgg atggatattg gggttatacc aaaaagcact
     1441 tatttttttg ccctcagctc tcaatttata caaaggaaca ataaaacaag gggcacaaca
     1501 gcaatgcagt aaaagttctt tttctgccat aatgtatagc tcaaatcatt cttcagaatg
     1561 atagttcttc ggttctaact ttgttctttg ttctccctgc tattaacagg aatccctgta
     1621 tcaccttaaa attcttttaa taaattggca gcaacaatgt tccgttgaat ctggttagtg
     1681 ccttcatata tctgtaatat tttggcatcg cgcatcattt tctctactgg atattctttc
     1741 atataaccat atccacctaa gatttgcacg gcatcggtag taactttcat tgccacatcc
     1801 gaagcgtaat atttgcacat agcgctttct ttggaataat ttctggctcc actatcaatc
     1861 attcttgctg tttgcattac taaagctctg gcagcttcaa tttgagtagc catatctgcc
     1921 agcataaatt gtatcccctg aaaactggaa atcggctttc caaattgctg tctttctttg
     1981 gaatatttta tagcagcttc aaaagcaccg cgagcaacac caacagcttg tgcacccacc
     2041 ataggacgag atttatcaaa aaccttcata gcagttataa aacctgtgcc ttcacgcccg
     2101 atcaaatttt ctgcaggaac tttacaatct tcaaaaatca attctcttgt agcggaagca
     2161 cggataccca ttttgttttc ttttttgcca aaattgaaac caggagtgcc tttttccact
     2221 aaaagacagg aacatcccct cgctcctttg gttctatccg tcattgcaaa aacacaataa
     2281 ataccagctt ctccgccatt agttatccac tgtttagtgc catttaaaat ataataatcg
     2341 ccttcttttt tagctgtagt ttcaattgct ccagcatcac tacctgcatt tgcttcggtt
     2401 aaagcaaaag cagcaagctt ctctcccgca gctatttgcg ataaatattt ctgcttctgc
     2461 tcttcactgc ctgcaattaa tatcggatac attcctaacc cagtagctcc aaatgctaaa
     2521 gatattccag catcaacagt acaaagttct tccgtaacta ctgccaaatc aacaactttg
     2581 ccactaattc cgccatattc ttcaggaatc aaaatagcga aaagatcgct ttgcttcatc
     2641 acttctatga tatcccaagg gaaaataccc tcttcatcat atttttcgga aacaggtttg
     2701 attttttcct cagcaatccg ccgtgctatt tcttgcattt ccaattgatc ttcagttaaa
     2761 aagtaatcca ttatgtaact cctctatatt tcaatttgtt atatgagtta tcattaagca
     2821 gaaatacctg aaaattgtca atctattttt tctccactta tttataagcc ttatattcaa
     2881 caatatcatc ccatacgatt cccttatccg atatgggaac gataagggaa ttatatgttc
     2941 tgcatatcac cagcaagaaa gttcaaagtg caggagttca aagtgcagga gttcaaagtg
     3001 cgggagttca aagtgcagga gttcaaagtg cgggagttca aagtgcagga gttcttagtt
     3061 caaagtgcag gggttcaaag tgcgggagtt caaagtgcgg aagttcaaag tgcaggagtt
     3121 cttagttcaa agttcttctt tcggtgactt cggtggctaa atattaaaaa tcctcttaat
     3181 cctaaaatcc ttaaatcctc gtttctaaaa ctaaatctgt gtaatctgtg taatctggtg
     3241 cgccacgaag cgttgcatgg gaagctggtg gaaatccagc ac
//
LOCUS       DAOSTH010000127         3052 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_29053_length_3052_cov_13.112779, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000127 DAOSTH010000000
VERSION     DAOSTH010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29053_length_3052_cov_13.112779"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..798
                     /gene="wrbA"
                     /locus_tag="PLB65_08170"
     CDS             190..798
                     /gene="wrbA"
                     /locus_tag="PLB65_08170"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HOQ78541.1"
                     /translation="MKVLVLFYSAYGHIYRMAEAVAEGARKVEGMEVELKQVPETLSK
                     DILDKIGATEAKKAFNHIPIAQIDDLTKADAIIFGFPTRFGSLPSQMKTFLDGTGSLW
                     AKGALIGKIGSVFTSTGAQHGGQESTILGFYPILIHHGMLITGLPYSFAGQQTMAEIS
                     GGTPYGASTIVGEGDRMPSENELEGARYQGWYVASLVAKMNK"
     gene            962..2269
                     /locus_tag="PLB65_08175"
     CDS             962..2269
                     /locus_tag="PLB65_08175"
                     /inference="COORDINATES: protein motif:HMM:NF024700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ78542.1"
                     /translation="MIIRFSVENCLSFNEKTSLEFFASKDRNLKEQLWISESSFIPNI
                     LKAAFIFGPNAGGKSNLIKAFHYAKSIVLGETNPLKNRTIYFKLCPDCPQKPIFLEFE
                     MLIKRNIYRYGFEILGGTIFREWLWKINSTGEHKIYERTYDKLQKNKISYGDNYKALQ
                     EDEYFNFVLKGTPENKLFLTETINRNIEQFRDVFEWFKKIVIIYPQSRLQSFNVLINN
                     NFLLQEYRKLMKLCDLGIDDISIEKIDADEVLNKLPSTIRDSLRMDLLGNITLKTDER
                     YSMNFKNGMQEAYRILISHKIPATESKVEFKINEESDGTNRIFDLIPVLQLLQEDAIV
                     LVDELDRSLHPLVSRAFVKALFTANKQHQGQFIVTTHDATLLDVKLLRRDLIWFIRKN
                     YQMESTLYSLHDFEKIRNDKALQKAYLAGLYGAVPIIIDEDEE"
     gene            2266..2859
                     /locus_tag="PLB65_08180"
     CDS             2266..2859
                     /locus_tag="PLB65_08180"
                     /inference="COORDINATES: protein motif:HMM:NF025084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RloB family protein"
                     /protein_id="HOQ78543.1"
                     /translation="MSYARKSQKRIPRRIYIFTEGSKTEPKYFREFIQYYKISQAQVK
                     VIGRETTASSPKSVIEAMDYYKKSNPVKTKNDIYCMVIDTDRWGKNLKEAVDDAHQRR
                     YLVALSNPCFEIWLLMHFQDANTILNNANLLMTKTEINTQIHSYPITGNNEIDYFPRT
                     DSAVETAEKLDPKPQQRLLEQIGSRVYSLMKILQEYI"
BASE COUNT         1065 a          488 c          571 g          928 t
ORIGIN      
        1 ctattaagaa gaagcagttt ataaaatcct ctaaatcctg aaatccttaa atcctaaaat
       61 ccttgtttct aaaattcact ccttacatcc ttgtttctca aattcacgca attcaaaact
      121 atagcttgtc agatgaaatt attggattat actattatca aagcaataaa acaaggagta
      181 taaacaaaaa tgaaggtatt agttctcttt tactctgctt acggacacat ttacagaatg
      241 gcagaagcag ttgctgaagg tgccagaaaa gttgaaggaa tggaagtgga actcaaacag
      301 gttccggaaa ccctcagtaa ggatattttg gataaaattg gtgctacgga agccaagaaa
      361 gcttttaatc atatcccgat agcccaaatt gacgatttaa ccaaagctga tgccattatt
      421 tttggttttc caactcgctt tggcagttta ccttcccaga tgaaaacatt tttagatggg
      481 actggttcgc tatgggcaaa aggtgcttta attggtaaaa ttggtagcgt ttttacttct
      541 accggagctc agcatggggg gcaggaatca acaattttag gtttttaccc gatcttaatt
      601 catcacggaa tgttaataac cgggctacct tattcctttg ctggacagca aactatggct
      661 gaaatttcag gaggcacacc ttacggagca tcaacaattg tgggtgaggg tgatagaatg
      721 ccttccgaaa atgaattaga aggtgccaga tatcaaggct ggtatgttgc ttctcttgta
      781 gctaaaatga ataaataaaa tacataccct aactgcgcat acaatagata cttaaagtgg
      841 aaatctctat tttatttaga aaagtttaga agtgcagaga aaactcttga cgcggaaaac
      901 tagataggag attatgcact taatgagaaa aattctccct aagtgaatat aagaggtggc
      961 tatgatcatc agattttctg tggaaaattg tctatctttc aacgagaaaa catctttgga
     1021 attctttgct tccaaagatc gcaatttaaa agaacaactc tggatttcag aaagcagctt
     1081 tattcccaat atattgaaag cagcttttat ctttggcccc aatgcgggtg gtaagtccaa
     1141 tctaattaaa gcatttcatt atgccaaatc cattgtttta ggggaaacga atcccctaaa
     1201 aaaccgaacc atatatttta agttatgtcc ggattgtcct caaaaaccga ttttcttgga
     1261 atttgagatg cttattaagc gcaacatata tcgctatggc tttgaaattt tgggagggac
     1321 aatttttcgc gaatggctgt ggaagattaa ttcaaccgga gaacataaga tatatgaacg
     1381 aacttatgat aaacttcaaa agaacaaaat cagctatggg gataattaca aagctctgca
     1441 agaagatgaa tatttcaatt ttgtgcttaa aggaacaccg gagaataagt tatttcttac
     1501 ggagacaata aatagaaata tagaacaatt tcgcgatgtt tttgagtggt ttaagaaaat
     1561 agtgataatt taccctcagt caagattaca atcctttaat gttttgataa acaataattt
     1621 tctcttgcag gagtatcgga agttaatgaa gttatgtgat ttgggaattg atgatattag
     1681 tatagagaaa attgatgcag acgaagtttt aaataaattg ccgagtacca taagagacag
     1741 tttgcgaatg gatttacttg ggaacattac tttaaaaact gatgaacgct attcaatgaa
     1801 ctttaaaaat ggtatgcaag aagcatatag aatccttata tctcataaaa taccggcaac
     1861 agaatccaaa gtggaattta aaattaatga agaatccgat ggaacgaatc gtatatttga
     1921 tctgattcct gtgttgcaat tattacaaga ggatgctatt gttttagtgg atgaacttga
     1981 tcgcagttta catcctttag tatccagagc atttgttaaa gctctattta ctgcaaataa
     2041 gcaacatcag ggtcagttca tagtaactac ccatgatgca actttattgg atgttaaatt
     2101 actgcgcagg gatttaatct ggttcataag aaagaattat caaatggaat ccacattata
     2161 ttcacttcac gattttgaaa agataagaaa tgataaggca ttgcaaaaag catacttagc
     2221 agggttatac ggagctgttc caattataat tgatgaggat gaagaatgag ttatgcccgt
     2281 aaatctcaga aaagaatccc caggcgcata tatattttta cggaaggtag caaaacggaa
     2341 ccgaaatatt tcagggaatt tattcaatac tacaaaattt cgcaggcaca agtaaaagta
     2401 ataggcagag aaaccacagc ttccagtcca aaatctgtta ttgaggcgat ggattattat
     2461 aagaaaagta accccgttaa gacaaaaaat gatatttatt gtatggttat agataccgat
     2521 agatggggta aaaacttaaa ggaagctgtt gatgatgccc atcaaagaag atatttagtt
     2581 gctttgtcca atccttgttt tgagatatgg cttttgatgc actttcagga tgctaataca
     2641 attttaaata atgctaatct gctaatgaca aaaacagaga taaatacaca aattcatagt
     2701 tatcctatta ctggcaataa tgaaattgat tactttccca gaaccgattc cgctgtagaa
     2761 acagctgaaa aacttgatcc aaaaccgcag caaagattat tagagcagat tggttccaga
     2821 gtgtattcac taatgaaaat actacaagaa tatatatgat tgcctattta ttctttattc
     2881 caatctccag atgatttgtt atattcgttc agggttggtt acttgttaga taattagcag
     2941 tcgaagagtt ggtctgctaa tatttccctt gacattccct caaactcgat tatattttac
     3001 ttatactgga ggtatatatg aatacacaaa gatttacaat aaagtcacaa ga
//
LOCUS       DAOSTH010000128         3003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_29669_length_3003_cov_12.645862, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000128 DAOSTH010000000
VERSION     DAOSTH010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29669_length_3003_cov_12.645862"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..2705
                     /locus_tag="PLB65_08185"
     CDS             195..2705
                     /locus_tag="PLB65_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78544.1"
                     /translation="MKKRVYLILAILILMLGSLSAKHSDKYLLANYTLLRCYQNVPGW
                     YYTLLDSIQAAKFNASVITVMPGDFPKRTDLLFNAMDERGLDVLHYDMAFNPDSKNPE
                     YGIEAFSIANYWRFEAEYDSTFKENLLDDKYFYNNSLTTGKQVYNELASGKYLLQLNN
                     GQPGYAFNKLEFRWQDKGYTNYLIGNEFRFIQREWAGNFKANPSVNDTLFVTIAFKCS
                     NIPTDPNAELMQFSFNGFDNTKNEHKVPHLNTLSSESGFTSKLTVNQYNKLPLISTDE
                     QGNMLWPHKEITLKVSLQDLHDAGLLEGGILWKYLLSNLNPQIYWNGKGILELDYVEF
                     EDTMNKRQKTDTELIQAVRKRVYDFAERYDNIKYFYLTDEPTQGQFSSYKRIHKDIFK
                     DIKHLTGGSSLFTCSILHRKYVIKPNNMMYDHIGLYSREVDPEIISFDIYPLWGWIQW
                     NNPEERRGIQRRLHYDMLEPYKYYKELCMKTGAKYMPCPQSYGEWVYTSPDTGFWGLL
                     RPPKYMQKCFQLLPLCYGADGILTYRINNKIYDATALKLRYQEFTTIDVAPSGELTFR
                     AGWDGLKEANEKILVYADYMNPKEWIDTDVIQTNGYQNPAKLESMLIKSVEVIPQKLV
                     QNGVDLYDGYVQCGLFKAAEKYPYFIMVNRRTEYISMENNPNKCDSLMYVHPDKLDDA
                     YLTAPPQTVRFYIDDNAKNILGEYVALYDPFDKSIFYSDKKCPEISIDPGDGRLLQMC
                     ATLPKKVTGKNELNYLAVLDGNLVLEKNAAVTVNPGCKLIIQDNSKIVVKKGAKLNIL
                     GETEIGDNVQIQIYKGGKVYLNETDCQWGKNSQITAVK"
BASE COUNT         1011 a          497 c          567 g          928 t
ORIGIN      
        1 agatcatttt agaattgcct aagcaggaag tgccagtaat cgatttagtt aagctgcgct
       61 tttttgctaa aaatcagatt aggataagta tcccttgaat atttgcttga cagctaccaa
      121 atagaaattg ttaaggtata aattgataat atgtgaaaaa atagaacttt atgagttcta
      181 taaggaaggt tcagatgaaa aaaagagtat atcttatttt ggcaattctg atcttaatgc
      241 ttggttcgct atctgctaag cattcagata aatatctttt agcaaattac actttgttaa
      301 ggtgttatca aaatgttcct ggctggtatt atactttatt ggattcaatt caggcagcta
      361 aattcaatgc ttctgtaata accgtaatgc ctggtgattt tcctaagaga accgaccttc
      421 tttttaacgc gatggatgag cgtgggttgg atgttctgca ttatgatatg gcatttaacc
      481 ctgatagtaa aaaccctgaa tatggtattg aggcattttc tattgctaat tattggcgtt
      541 ttgaagctga atatgattct actttcaaag agaatctatt ggacgacaag tatttttata
      601 ataattcctt aacgactggc aagcaagttt acaatgaact tgcttctggt aaatatcttt
      661 tgcaactaaa taatggtcaa cctggttatg cttttaataa attagaattt cgctggcagg
      721 ataagggcta tacaaattat ttaatcggta atgaattccg ctttattcaa agagaatggg
      781 cgggtaattt taaagcgaat ccctcagtaa acgatacttt atttgtaacg attgctttta
      841 aatgttcaaa tatccctacg gatccaaatg cagaattgat gcaattttct tttaacggtt
      901 ttgataatac taaaaatgaa cataaagttc cacatctgaa cacactatct tccgaatctg
      961 gctttaccag taaactaaca gtaaatcaat ataataagct acctcttatc tctacagatg
     1021 aacagggaaa tatgctctgg cctcataaag aaatcacttt aaaagtttct ttacaggatt
     1081 tacacgatgc cggccttttg gaaggaggca ttttgtggaa atatctactt agcaatctca
     1141 atccccaaat ctattggaac ggaaaaggaa ttttggaatt ggattatgta gaatttgaag
     1201 acacaatgaa taaacgccaa aagacagata ccgaactgat tcaagctgtt cgcaaaagag
     1261 tttatgactt tgcagaaaga tatgataaca ttaaatattt ctatcttacc gatgagccaa
     1321 cccaaggtca attcagttct tataagcgta tccataaaga tatttttaaa gatataaaac
     1381 atttaaccgg tggttcatct ctattcacct gttccatttt gcataggaaa tatgtaataa
     1441 aacctaataa tatgatgtat gaccatattg gattatactc tcgcgaggtt gatcccgaaa
     1501 tcatctcttt cgatatctat cctctttggg gttggatcca atggaataac cctgaagaaa
     1561 gaagaggaat ccaaagacgc cttcattatg atatgttgga accatataaa tactataaag
     1621 aactctgtat gaaaaccggc gcaaaatata tgccctgtcc ccaatcttac ggtgaatggg
     1681 tttatacaag ccctgataca ggtttttggg gattgctgcg cccgcccaaa tatatgcaaa
     1741 agtgttttca acttttacct ttatgttatg gagccgatgg tattttaact tacagaatca
     1801 ataacaaaat atatgatgcc acagctttaa aattgcgcta tcaagaattt accactatag
     1861 atgtagctcc ctcaggagaa ttaactttcc gcgctgggtg ggatggtttg aaagaagcta
     1921 acgaaaaaat cctggtgtat gctgattata tgaaccccaa agaatggatt gacacggatg
     1981 ttattcaaac aaatggctat caaaaccctg ccaaactaga atcgatgcta ataaaatcag
     2041 tagaagttat acctcaaaaa ttggtgcaaa atggagttga tctctatgat ggctatgtgc
     2101 aatgcggact ttttaaagct gccgaaaaat atccttattt cataatggtt aatcgccgaa
     2161 ccgaatatat ctctatggaa aataatccta ataaatgcga tagcttgatg tatgtgcatc
     2221 ctgataaatt ggatgatgcc tatttaaccg ctcctcctca aacagtgaga ttttatatag
     2281 atgacaacgc caaaaatatt ctgggagaat atgttgccct ttatgacccc ttcgataaaa
     2341 gtattttcta ctcggataaa aaatgtcccg aaataagtat tgatcctgga gatggacgcc
     2401 ttttgcaaat gtgcgcaact ttacctaaaa aagtaacagg taaaaacgaa cttaactatc
     2461 tggcagtttt agatggtaat cttgttttgg aaaagaatgc tgctgtgaca gttaaccccg
     2521 gttgcaaatt gattattcaa gataacagca aaattgtggt taaaaaaggt gctaaactga
     2581 acattcttgg cgaaactgaa attggtgata atgtccaaat ccaaatatac aaagggggaa
     2641 aagtatacct aaatgaaact gattgccaat gggggaaaaa ttctcagata acagctgtga
     2701 aataagcaaa agacaaagtt cagagttcag agttaatctt gcggttttgg aaacaaggat
     2761 tttaggatta gttcaagcag attacgctga tgacgcagat ctgatttatc gaaacaagga
     2821 tttaaggatt ataggattta gaggattatt gttttataaa tggcgattat taaagagggt
     2881 tatggaaagc ccctcagagt gacacaatta tagcgacagt ggcgtaaata atctgtcagc
     2941 tttaagcagt cggcggtcag ctttaagtca aaatcggcat tttgtcatcc tgtcagcttt
     3001 aag
//
LOCUS       DAOSTH010000129         3003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_29670_length_3003_cov_11.787992, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000129 DAOSTH010000000
VERSION     DAOSTH010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29670_length_3003_cov_11.787992"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            112..297
                     /gene="secE"
                     /locus_tag="PLB65_08190"
     CDS             112..297
                     /gene="secE"
                     /locus_tag="PLB65_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HOQ78545.1"
                     /translation="MKFEKISRFFRDVYSEMKCVSWPTKADLKEGTLVVIIMSAIVAI
                     FLSLVDFGFTKIMELVF"
     gene            317..850
                     /gene="nusG"
                     /locus_tag="PLB65_08195"
     CDS             317..850
                     /gene="nusG"
                     /locus_tag="PLB65_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HOQ78546.1"
                     /translation="MKWYVIHTYSGHENKVKTAIEKGLSGTPMEKNVGRLLVPVRKTF
                     VIREGKKIERDKKIFTSYVIMEADMTPELKAYILQIAGVTSFLGISKEHKEPIPLSQE
                     EVDRLLGITDPNRDVKVFSYMPGDMVKVISGPFTDFEGIVQKTTENGTKLIIEVTVFG
                     RRTPVELNANQVELIKK"
     gene            874..1305
                     /gene="rplK"
                     /locus_tag="PLB65_08200"
     CDS             874..1305
                     /gene="rplK"
                     /locus_tag="PLB65_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HOQ78547.1"
                     /translation="MAKPKDAVAMIKLQLPAGKATPAPPVGPALGQVGVNIPEFCRQF
                     NEKTADQPGMVFPVVIYVAKNKSFTFEIKTPPASVLIKKEAGLATGSATPNKVKVGKI
                     NREQLKNIAQMKMKDMNCFSLESAMNMIAGTARSMGVTIED"
     gene            1344..2048
                     /gene="rplA"
                     /locus_tag="PLB65_08205"
     CDS             1344..2048
                     /gene="rplA"
                     /locus_tag="PLB65_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HOQ78548.1"
                     /translation="MKHSKRYNVAYSMFDRQKRLDLNEAVKLIKSLPASKFDETVEVH
                     FNLGVDPRKADQQIRNSLVLPHGTGKEMRVLVFAEGDKAEEAKQAGADYVGVDDLIEK
                     ISGGWFDFDVVIATPNLMGRIGKLGRVLGPRGLMPNPKVGTVTMDVSKAVQEAKGGKV
                     TYRVDKFGNLHIPAGRLSFDEDKLKENIKAIIAAVLRERPSTLKGIYIKSITLCTTMS
                     PGIKIQVASATLEARS"
     gene            2062..2598
                     /gene="rplJ"
                     /locus_tag="PLB65_08210"
     CDS             2062..2598
                     /gene="rplJ"
                     /locus_tag="PLB65_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HOQ78549.1"
                     /translation="MVQSIKYDIVTDLTARLSGAKAIVLVDYKGINIEQVNQLRNRFR
                     ESQVDYFVQKNTLIKIALNGLGITELDPYLNGPTAVAVCKMDEVSPARELTKFLKEVM
                     ENKNYPSFKVGYIAGHIFDAVELTALAKLPSREELLAKVLSCMNAPLSNFVSINQGII
                     RKFVYALDAIVKKQAEAS"
     gene            2632..>3003
                     /gene="rplL"
                     /locus_tag="PLB65_08215"
     CDS             2632..>3003
                     /gene="rplL"
                     /locus_tag="PLB65_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HOQ78550.1"
                     /translation="MSDKKQQVIDLIKEMTVLELSELVKEMEEVFGVSAAAPVAVVAA
                     TQGAAEGGVKEEQTEFDVILTSAGEKKINVIKVVREITKLGLKEAKDLVDSAPKMVSE
                     KVSKEEAESIRKKLEEAGATVE"
BASE COUNT         1019 a          504 c          676 g          804 t
ORIGIN      
        1 ctccaaaacc gggggttgcg ggttcgattc ctgcctgtcc tgccacaaca ttttatagaa
       61 atactatatc ggaggcaggt tctacctcca ggatgaaagg gaataaagat tatgaaattc
      121 gagaaaatta gtcgtttctt tcgagatgta tattccgaaa tgaaatgcgt aagctggcct
      181 acaaaagcag accttaagga agggactcta gtagttatta ttatgtctgc aattgtagct
      241 atatttttat ccttggtaga ttttggtttt accaaaatta tggaacttgt attttaaaac
      301 aatggatgaa aaacaaatga aatggtatgt gattcatacc tattcaggcc acgaaaataa
      361 ggttaaaaca gccatcgaaa aaggtctgtc tggcactcct atggaaaaaa atgtaggacg
      421 cttactagtc ccagtgcgca agacctttgt gattagagaa ggcaaaaaga tagaacggga
      481 taaaaaaatc tttacttcct atgtgattat ggaagcagat atgacacctg agctgaaagc
      541 ttatattctt caaattgcag gggtaactag ttttttagga atcagcaaag aacataaaga
      601 acccataccc ttgtcacaag aagaagtgga ccgcctttta gggataactg atccaaaccg
      661 tgatgttaag gtcttttctt atatgccagg agatatggtt aaagttattt ctggtccttt
      721 tacggatttt gagggtattg tacaaaagac cacggaaaac ggaactaaac tgattataga
      781 agtaacagtg tttggacgca gaaccccggt tgaacttaat gccaaccagg tagaactaat
      841 taagaaataa agaagataaa atagaggtaa taaatggcta aacctaaaga tgctgtagcg
      901 atgataaaac tacaacttcc agctggaaaa gcaactccgg cacctcctgt aggacctgcc
      961 ttagggcaag taggagtgaa tattccagaa ttttgccgtc agtttaatga aaaaacagct
     1021 gatcagccgg gcatggtttt tccggtagta atttatgtag cgaagaataa atcttttaca
     1081 tttgaaataa aaacccctcc agcttcagta ctgattaaaa aagaagcagg tctggctacc
     1141 ggtagcgcta cccccaataa agtaaaagtt ggaaaaatta accgcgaaca gcttaaaaat
     1201 atagctcaga tgaaaatgaa agatatgaat tgcttttccc ttgaatctgc tatgaatatg
     1261 atcgctggaa ctgcacgaag tatgggtgtt acaattgaag actgatactt cgatgcagga
     1321 gatagtattc ataaggagag tcaatgaaac atagtaaaag gtataatgtt gcctattcga
     1381 tgttcgatcg tcaaaaaaga ttagacctta acgaagctgt taaacttata aagagtttac
     1441 ctgcttccaa gtttgacgaa acagttgagg tccattttaa tttgggtgtc gatcctcgaa
     1501 aggcagatca gcaaattaga aactctcttg tcttgcctca tggaactggt aaagagatgc
     1561 gagttttagt ttttgctgaa ggtgacaagg cagaagaagc caaacaagct ggtgcagatt
     1621 atgttggtgt ggatgatctc atcgaaaaaa tatcgggcgg atggttcgat tttgatgtgg
     1681 tgattgccac tcccaattta atgggacgca tcggaaaact gggacgcgtt ttaggcccca
     1741 gaggattaat gcccaaccct aaagtaggaa ctgttacaat ggatgttagt aaagcagtgc
     1801 aggaagctaa aggtggaaaa gttacttacc gtgttgataa gtttggtaat ttacatattc
     1861 ctgcaggtag acttagcttt gatgaagata aactaaaaga aaacatcaaa gctattattg
     1921 ctgctgtgct tagagagaga ccttctactt taaagggtat ttatattaag agtataaccc
     1981 tctgcacaac tatgagtcct ggaatcaaaa ttcaggtcgc aagcgcaacc ttggaagcaa
     2041 gaagctaagg ggagtatgaa gatggttcaa agtattaagt atgacatagt gacggacttg
     2101 acagcaagac taagtggcgc aaaagcgatc gtattggtag attacaaggg aataaacatc
     2161 gagcaagtga atcaattgcg caaccgcttc agagaaagtc aagtggacta ctttgtccaa
     2221 aaaaataccc taattaaaat tgctttaaat ggtttgggta ttacagaatt agatccgtat
     2281 ctcaacggac caaccgctgt ggcagtatgt aaaatggatg aagtatcccc tgcccgcgag
     2341 cttactaaat tcttaaaaga ggtgatggag aataagaatt atccttcgtt caaagtcggt
     2401 tatattgcag ggcatatatt tgatgctgtt gaattgactg ctttggcaaa acttccatcc
     2461 cgcgaagaac tacttgccaa agtgttaagc tgtatgaatg ctccgcttag caactttgta
     2521 agtattaatc agggtatcat ccgcaagttt gtttatgctc ttgatgccat agtaaaaaaa
     2581 caagctgaag ccagctaaaa aaaaattatt catatatccg atggaggaac aatgtccgat
     2641 aaaaagcaac aagtaattga tctaattaag gagatgacag ttcttgagct ctctgaattg
     2701 gtaaaagaga tggaagaggt ttttggtgtt tccgctgctg ctcctgtagc tgttgttgcc
     2761 gcaacacaag gtgctgctga aggtggagta aaagaagaac agacagaatt cgatgttata
     2821 ttaaccagtg ccggcgagaa aaaaatcaat gtgattaaag ttgtaagaga gataaccaag
     2881 cttggtctca aggaagcaaa agaccttgtt gatagtgcac ccaagatggt ttctgagaaa
     2941 gttagcaagg aagaagcaga atcaattagg aaaaaactcg aagaagccgg tgcaactgtc
     3001 gaa
//
LOCUS       DAOSTH010000130         2991 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_29814_length_2991_cov_19.773161, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000130 DAOSTH010000000
VERSION     DAOSTH010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29814_length_2991_cov_19.773161"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(313..987)
                     /locus_tag="PLB65_08220"
     CDS             complement(313..987)
                     /locus_tag="PLB65_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HOQ78551.1"
                     /translation="MKTNTFLLIMLLLLLAIGLPSQTLPKIQQGMIGGLNIANFSGDD
                     IEDLDDAGFTINNRTLFHLGLTGTTNITSNLDLRGEIALSMNGSKWKVNLDFEDEDFD
                     TVPFTITHTMYMIQAPVSLILNFPLPNSAVKPYLGAGLSASIPVSSSWEISALGEDEE
                     GDYTDEEFAELSSFVIGYQLNLGLDFNKILCEFRYERGLTPVFDNSDIDETFYSNFKF
                     LVGFRF"
     gene            1209..>2991
                     /locus_tag="PLB65_08225"
     CDS             1209..>2991
                     /locus_tag="PLB65_08225"
                     /inference="COORDINATES: protein motif:HMM:NF027446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4836 family protein"
                     /protein_id="HOQ78552.1"
                     /translation="MNEELKEQMQDQVSEESSETEEQVPAVLEGKRKKKYALIPVLII
                     LFIILVIIAGYFYATNSPQIRHISKNALVVAKVDIPKLIKKSGAMKKGEIDEEIISAL
                     EDYNLEALVKDPRSTGLITAKPSYLFGEFDTKENIPYIFLVMPIANAEKVYSFAKNIA
                     LPNGKELPVSKNSGIYEMEINGLSCMWAGKSMVMGMTDPDKDNDLSKKVKNYLTQDKS
                     ACITANKDFPSRGLRGHDITLWANLDGMAHIAQKGLKDAQGNVNRLISQRKKREELSN
                     YYYESNLRYYYNYWEIMNQIRVDYPENVFESVLLALVYGGKIPMDAVIDLPTNLYKTY
                     FSFFADFDKGKLVSSVKFETTPERQKKLESVITNAVNVKDLYPYVPTEGLIMLSSVKT
                     NYPKLYALSEKRIDKLTKSITNPEISKFMKHLNKLADGSGIIAINILDEKEDPFITLV
                     ASVKNNKGVQDLLKDMGREGMLSKDGKYYYLDDLIFFFEDNVMICTTNNNNYKKSNRG
                     LEGTLAREMNKNPLNMTMDFAVLKDLKHVFSDEEEDALEMFAKLNANVKTNSGTPNEL
                     KVELTFSDKKHNCLKQVKDYLSDIDNFW"
BASE COUNT         1026 a          477 c          582 g          906 t
ORIGIN      
        1 ttaatttaac tggcggatta tgtcaagaaa aaaggttgag aaggttgaga aggttaagat
       61 acattaaaag cccggagggc gacacaagga tagcgatggt ggcgtaagcc cctcgtaaaa
      121 atgaaagcca cgagtatttt ttgctttttt tctttatttc ttaaaaaagc cccaacaggc
      181 gacacaagga tagcgatggt ggcgtaagcc cctcggaaaa ttaatttacc ttaaaatccg
      241 cgaattacac aaaatctgcg taatctgcgt gaaacaattt cataaagaac attgtcctcc
      301 gtctttatta ctttaaaaac ggaagccaac aagaaatttg aaattgctat aaaatgtctc
      361 gtctatgtca ctattatcaa atacgggagt gagacctctt tcatatctga attcacaaag
      421 aattttattg aaatccagac ccagattgag ttggtagcca ataacgaagg aagataattc
      481 agcaaattct tcatctgtat aatcaccttc ttcatcttca cctaatgcgc taatttccca
      541 gctggaacta acgggaatac tggcagaaag tccggcaccg agatatggtt taacagctga
      601 attggggagg ggaaaattta ggatcaagga taccggtgcc tggatcatat acatagtatg
      661 ggtaatggta aaagggactg tatcaaaatc ttcatcttca aaatctaaat taactttcca
      721 tttggaacca ttcatactca aagcaatttc tccacgcaga tcaagattac tggttatatt
      781 tgtagtacct gtaagcccga gatgaaaaag ggttctgtta ttgatagtaa aacctgcatc
      841 atcaaggtct tcaatatcat ctccgctaaa attagcaatg tttaaacctc cgatcatacc
      901 ttgttggatt tttggtaaag tttgggatgg aagccctatt gccaacagca ataaaagcat
      961 tattaacaag aaagtattgg ttttcataat tcttcccttt ttgcattaga aatttatgct
     1021 tctgtttatt cttttaacct ttgtattttt attagtttta ctgtcaatct tttttctgct
     1081 ccttatagga tataagatta gtaactaacc ataaaaaaag aaaaaccttg acattatttt
     1141 agagaaatac atatttgcat ttgtctttat ttaagctgaa gaaaaaataa ataactatgg
     1201 aggttcaaat gaatgaagaa ttaaaagaac aaatgcagga tcaagtttcg gaagaaagtt
     1261 ctgagaccga agaacaagtt cctgcagttt tagagggtaa gcgtaaaaag aaatatgctt
     1321 taatccctgt tctaatcatt ttatttatca ttcttgtgat tattgctggt tacttttatg
     1381 caacaaattc tccccaaatt cgtcatatca gtaaaaatgc tttggtagta gcaaaggttg
     1441 atattcccaa gctcattaaa aagtccggag caatgaaaaa gggtgaaatt gacgaggaaa
     1501 taatttcagc cctggaagat tacaatttgg aagcattagt taaagacccg cgtagcacgg
     1561 gactaataac tgctaagcca tcataccttt ttggtgagtt tgatacgaaa gaaaacattc
     1621 cctatatttt tttggttatg cctattgcta atgcagaaaa ggtgtattcc tttgctaaaa
     1681 atatagctct acccaatgga aaggaacttc ccgttagtaa aaattctggt atctacgaaa
     1741 tggaaataaa tggtctttcc tgtatgtggg caggcaaatc tatggttatg ggaatgaccg
     1801 atcctgataa ggataacgac cttagtaaga aagtgaaaaa ttacttaact caggataaat
     1861 ctgcctgcat tactgccaat aaggattttc ccagcagagg cctaagaggt catgatatta
     1921 ccctttgggc taatctagat ggaatggctc atattgctca aaaggggtta aaagatgctc
     1981 aaggaaatgt aaataggtta atttcccagc gcaagaaacg agaagaatta agtaattatt
     2041 attatgaaag taacttaaga tattattaca attactggga gattatgaat caaattagag
     2101 tggattaccc ggaaaatgtt tttgaaagtg ttcttttagc attagtttat ggtggcaaga
     2161 ttccaatgga tgcagtaatt gatttgccta ctaatttgta taagacctat ttctctttct
     2221 ttgccgattt tgataaggga aaattggttt cttcggtaaa atttgagacg accccagaaa
     2281 ggcaaaagaa actggaaagc gttattacca atgccgtcaa tgtgaaagac ctttatcctt
     2341 atgttcctac cgaaggattg attatgctga gctccgtaaa aaccaattat ccgaaacttt
     2401 atgcactttc ggaaaagaga attgacaagc tgacgaaaag tattaccaat cctgaaatta
     2461 gtaagtttat gaaacatttg aataagctgg cagatggttc aggcataata gctataaata
     2521 ttttggacga aaaggaagat ccctttatta ctcttgttgc ttcagtaaag aacaataagg
     2581 gagtgcagga tttgctgaag gatatgggac gtgaaggtat gcttagcaaa gatggtaaat
     2641 attactattt ggatgattta atcttctttt ttgaagataa tgtgatgatt tgcactacca
     2701 ataataataa ctataaaaag agtaaccgcg gtttggaagg aactcttgcc agagaaatga
     2761 ataaaaatcc gttaaatatg acgatggatt tcgcggtttt aaaagattta aaacatgtat
     2821 tctccgatga agaagaagac gctttggaaa tgtttgcgaa attgaatgcg aatgttaaaa
     2881 ccaatagcgg aactcctaac gaactgaaag tggaacttac attcagtgat aaaaagcata
     2941 actgcttgaa acaggttaag gattatctaa gcgatattga caatttctgg g
//
LOCUS       DAOSTH010000131         2972 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_30071_length_2972_cov_14.675694, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000131 DAOSTH010000000
VERSION     DAOSTH010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30071_length_2972_cov_14.675694"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            690..1175
                     /locus_tag="PLB65_08230"
     CDS             690..1175
                     /locus_tag="PLB65_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78553.1"
                     /translation="MNLWMYPKQKNPKIEKSRKGKNYALWCLSNSLFIFFNPSKSVLQ
                     KSRLNSPATSGTINQDKCPTRQKNNSNFSQKPYKHNGNLIIFSEIRNALKTGKRNLTN
                     LLHSFCNHHRTLLLKHSAKETNSVFPYKLKPSKTNLAFLCLPFYKPKTLIKIPKVMPI
                     M"
     gene            1227..>2972
                     /locus_tag="PLB65_08235"
     CDS             1227..>2972
                     /locus_tag="PLB65_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HOQ78554.1"
                     /translation="MKKTIIFLTFLLFIAGMFGVEVIIGTGTTYNTYSSYPAVYGGYY
                     MNAREQYIVTPAEFAANGGGAGNITSIAFNVNNVNGCGSLPNFTIRMGNTTQTAFTTT
                     TFITGLSQVYNVASYQPVAGWNTHIFNSAFNWDGTSNVVIEVSFDFQDDWTENTSTYY
                     TSTSDYKAMYYRNDYTAWNTVTTGTQSYNRPNMKFNIAAGGPMAPFPALLVSPANGAS
                     MLPLNTTLNWASGGGNPTGYRLYFGTTTPPLLYDDLGNVTTFSPVLDYETTYYWQIVP
                     YNGEGDAQNCPIWSFSTAAEGMVLIGTGTSTQRQPFGIWYGFERSAAIYTNAQIGNYG
                     VLDKVAWNCATANPSQVPYNIYAMQTTDTEFTTAMTWDAFTANATLVDTGTHTFDTTG
                     WHIFELTTPFTYTSGNLVIGVETNYGGGGTSPYPYFYYTAGTASRHQYWNADNNPPTA
                     NGYLNAYVPNIVLHLGALSEEPVFNVSPASWDFGQVQINTIKTKNFTISNIGGGTISV
                     SSIIIAGDDYYTLTTNPAPVNLTAGQPATFTVQYAPTAVGNHNGTVTITDNRGTTIVD
                     LSAVCYDPTIYTFPYT"
BASE COUNT          950 a          578 c          546 g          898 t
ORIGIN      
        1 aaaattgtga gttaaaacaa gaaacaagca caccgttaac cgagctacaa caaaccaacg
       61 aatcccacgg tcacggaaaa aatatatatt tagccgctcc agaaatgtgg ggtaggaatt
      121 gttcgtaatt tgtggcttcg ggggattctt ttgttttaac tctaaaccga gcttcagctt
      181 tgaaagtaga agacagtgaa taacatttct tgttacctca aaatctcgtt gcaatcacaa
      241 aagaatcggt ttagagtcaa aactccaccg cgtctgcata tagttgcaaa cacagcaaga
      301 gccggtttag agtcaaaacc tcacctcaaa actccccgaa accttatttt ttacttgaca
      361 taaattcagg aactattatg aaggatttat agtatgatga actcatcttg aagttgaagt
      421 aatatcctta aaactggtaa gttaagaaga atggcttaat aaaaaacagt cattttacta
      481 tcaacttgcc agaaggaaaa tttggattat attatcttaa gacgaagaag aaaacggttg
      541 aatacaaaat ttctatgttg tatttagccg gtagagatat gagtcctcgc taccgaaagg
      601 atagtaaaaa gataaaaact gcttaggtag tgatgaacta tcagccaagg aacgataaga
      661 tgtcgttctt ccgtagcaaa ggagtttaga tgaatttgtg gatgtaccca aagcagaaga
      721 accctaaaat agaaaaaagc cgaaaaggga agaactatgc actttggtgt ctatccaatt
      781 ctttatttat tttctttaac ccttcaaaat cagtcctcca gaaaagcaga ttaaattccc
      841 ccgccacttc aggaactatc aatcaggata agtgccctac aagacaaaaa aacaatagca
      901 atttttctca aaagccatac aagcataatg ggaatttaat cattttttca gaaatacgca
      961 atgcgcttaa aacaggaaaa cgcaatctta cgaacttatt gcactctttt tgtaaccatc
     1021 atcgcacatt attactaaaa cacagtgcaa aagaaacgaa tagcgttttt ccctataagc
     1081 ttaaaccgtc aaaaacaaac ctggcattcc tttgcctgcc attttataag cccaaaacct
     1141 taatcaaaat accaaaggta atgcctatta tgtaacaaat taaacaaaat cttcaagaaa
     1201 aaacatctaa aaaaggagtt tttgttatga agaaaactat aatcttctta acattcctgt
     1261 tgtttattgc aggaatgttc ggtgtggaag taataatcgg gacgggaact acctataata
     1321 catatagtag ttatcctgca gtttatggcg gatattatat gaatgcacga gagcagtata
     1381 tagttactcc tgctgaattt gctgctaacg gtggtggtgc cggaaatatt acttcaattg
     1441 cctttaatgt aaacaatgtt aatgggtgcg gttccttacc caatttcact attcggatgg
     1501 ggaatactac tcaaactgca tttacaacta ctacctttat tactggtctt agccaggttt
     1561 ataatgttgc ttcctatcaa ccggttgccg gctggaatac tcatattttt aatagtgctt
     1621 tcaattggga cggaacttct aatgtagtaa ttgaagtttc ctttgatttc caggatgatt
     1681 ggactgaaaa tacaagtact tattatactt caaccagcga ctataaagca atgtattatc
     1741 gtaatgatta tactgcctgg aatacagtca ccacaggaac ccagtcctat aaccgtccca
     1801 atatgaaatt taatatagca gcaggaggtc ctatggcacc ttttcctgct ttattggttt
     1861 caccagctaa tggagcatca atgcttcctt taaatacaac tttgaactgg gcttccggag
     1921 gtggaaatcc cactggttac aggttgtatt ttggaactac aacaccccct ctcttatatg
     1981 atgacttggg taatgtaaca acctttagtc ccgttttgga ttatgagaca acttattatt
     2041 ggcagattgt tccttataat ggtgaaggtg atgctcagaa ttgtcctatc tggagttttt
     2101 ccactgcagc tgaaggaatg gttctaatag ggacggggac atcaactcag cgtcagccct
     2161 ttggaatttg gtatggcttt gagcgttcag ctgctatata taccaacgct caaataggaa
     2221 actatggagt gttggataaa gttgcctgga attgtgcaac agctaaccct tctcaggttc
     2281 cctataatat ctatgcaatg cagacaactg atactgagtt tactacagct atgacctggg
     2341 atgctttcac agcaaatgct actttggtag atacaggaac tcataccttt gatactaccg
     2401 gttggcatat ctttgaactt actactcctt tcacctatac ttcgggtaat ttagttattg
     2461 gagttgaaac aaattatggt ggtggcggaa caagtcccta tccttatttc tattacactg
     2521 ccggaacagc ttccaggcac caatactgga atgcagataa taatcctcct actgctaatg
     2581 gatatctaaa tgcatatgtg cctaatatag tattgcatct tggagctctt tctgaagaac
     2641 cggtttttaa tgttagccct gccagttggg attttggaca ggtacagatt aacactatta
     2701 aaactaagaa ctttacgatt agtaatatag gtggtggtac tattagtgtg tcttctataa
     2761 ttatagcagg agatgattat tatacactga ctaccaatcc tgcccccgta aaccttacag
     2821 ctggtcaacc tgctactttt actgttcagt atgctcctac tgcagttgga aatcacaatg
     2881 gcactgttac tattactgat aacagaggta caaccattgt tgatttgagt gctgtatgtt
     2941 atgatcccac aatctatact ttcccttaca ct
//
LOCUS       DAOSTH010000132         2961 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_30218_length_2961_cov_12.633517, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000132 DAOSTH010000000
VERSION     DAOSTH010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30218_length_2961_cov_12.633517"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2248)
                     /locus_tag="PLB65_08240"
     CDS             complement(<1..2248)
                     /locus_tag="PLB65_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78555.1"
                     /translation="MKQLSLLLVMISMVCFAWATIDEHYEFFATTGTYTPITGTNAGI
                     SSDDVLSDIIPIGFTFSYGDFSYSEVKISSNGWIGLGTSQTYSNLSNSLNSTTYQPVV
                     APLWDDLSMVSGTVSYLMSGTAPNRVFIIQYENAKWYYSGPVGFNFQVQLFETGKISF
                     IYGSNPGIPNNPSASIGINMSPGGVGWFYSVNPTDGSASTTIETSNISTFPNQGTVFE
                     FNPVIPQPNDLAAIAISGNITPSVGLTANYTITIRNRGTNPQSTYQVQLIDSNGTELG
                     SVNGATIQPNQNLDFQVSWIPSVGGPLVIRGKVILAGDQNPNNDLSYPINIYIQPEGV
                     QAVTIADGTETMRIPMDFYFRNSLSETIYLSDELGFVSGAITALAFYNNFSESPANGA
                     TKIWLGSTNVQNLSGGWIPSTQMTLVFDGNITYPMGANPITIPLQTPYMHTPGNLVMM
                     VQRPMDTTWYSSTDNFYGMTMGSNRTLNIFNDGTAYDPANPPGGTTPINQVPKITIFY
                     TGQLIENDLGCLSITGNTVPTVGISSDYVITVKNNGIAAQTNYSIKLMKEGGIELVSV
                     PGPTINSLQTLSVTVSWTPTQAGSTYVYGQVVLTGDEFATNNQTAPLNINVQPEGVQA
                     VTIADGTETFRIPMDFFYCTSLCEVLYYADELGFVSGTITSLALYNNFSGTPANGATK
                     IWLGSTNVQDLSGGWIPSTQLTLVFDGNVNYPSGTNTIIIPLQMPYMHTPGNLVMMVQ
                     RPMDTTWYS"
BASE COUNT          941 a          548 c          601 g          871 t
ORIGIN      
        1 acgaatacca ggtagtatcc atcggacgct gaaccatcat aactaaattt ccaggggtat
       61 gcatataagg catttgtaag gggataataa tagtatttgt ccctgatgga taattgacat
      121 ttccgtcgaa aactaaggtt aattgtgtgg aaggaatcca tccaccactt aaatcttgaa
      181 cattagttga tcccaaccaa atcttggtag caccattggc aggagttcca gaaaagttat
      241 tatataaagc caaggaagtt attgttccac ttacaaaacc tagttcatct gcataatata
      301 gaacttcgca caaggaagta caataaaaga aatccattgg gattcggaat gtttctgtcc
      361 catcggcaat agtaactgcc tgaacacctt ctggttgaac attaatgttt agaggtgcag
      421 tttgattatt tgtggcaaat tcatcaccag ttaaaactac ttgaccataa acataggtag
      481 atccagcctg ggttggagtc caagaaaccg taacagaaag ggtttgaaga gaattaatag
      541 taggtcctgg cacagagaca agttcaattc ctccttcttt catcaatttt atactatagt
      601 tggtttgagc tgcaatacca ttgtttttta cagttattac ataatcagaa gaaatcccta
      661 ctgtgggaac tgtatttcca gtaatgctaa ggcagccaag atcattttca attagttggc
      721 ctgtgtagaa aattgttatc ttaggcacct gattgatcgg agttgttcct ccaggaggat
      781 tagcaggatc ataagctgtg ccatcgttaa atatatttag ggtgcgatta ctgcccatag
      841 tcattccgta aaaattatct gtggacgaat accaggtagt atccatcgga cgctgaacca
      901 tcataactaa atttccagga gtatgcatat aaggcgtttg caagggaatg gtaatggggt
      961 tggcacccat gggataggta atattgccat caaagactaa agtcatctga gtggaaggaa
     1021 tccatccacc acttaaattc tgaacattag ttgagcccag ccaaattttt gtagctccat
     1081 tggcagggga ctcagaaaag ttgttgtaga aagccagagc tgttatagct ccgctgacaa
     1141 agccaagttc atcggataaa tagatagtct cagagagaga attccgaaag tagaaatcca
     1201 tcggaatacg cattgtttct gtcccatccg caatagtaac tgcctgaaca ccttctggct
     1261 gaatgtatat gtttatagga taacttaggt cgttatttgg attttggtca ccagctaaaa
     1321 taactttacc gcgaataacc agtgggcccc ctacacttgg aatccagcta acctgaaaat
     1381 cgagattttg atttggctga atagttgctc cgttaactga accaagctcg gttccgttgc
     1441 tatcaattaa ttgaacttga taagtacttt gtggattagt tccccggttg cgaatcgtta
     1501 ttgtgtaatt agctgttaaa cctacagagg gagtaatgtt cccagaaata gcaatagcag
     1561 ctaaatcatt aggttgaggt attacaggat taaattcaaa aacagttccc tgattgggga
     1621 aggtagaaat attactggtt tcaatagtag tagaggctga accatcagtt ggatttacgc
     1681 tataaaacca gccaacgcca ccagggctca tattaatacc tatggaggca gaaggattat
     1741 ttggtatgcc tgggttggag ccataaataa aactgatctt gcctgtttca aacaactgaa
     1801 cctgaaaatt aaagccaaca ggaccagaat aataccactt ggcattttca tattgaataa
     1861 taaaaacacg attgggagca gttccagaca taagataact tactgttcca gaaaccatac
     1921 tgagatcatc ccaaaggggg gcaacgacag gttgatatgt agttgaatta agcgaattac
     1981 tcagattgct gtaagtttga gaagttccca aaccaatcca tccatttgag gagattttta
     2041 cttcgctata agaaaaatct ccataactaa aggtgaatcc aattggaata atatccgata
     2101 gtacatcgtc agatgaaatt ccagcattag ttccggtaat cggtgtgtat gttcctgtag
     2161 tggcgaaaaa ttcataatgt tcatcaatcg tcgcccaggc aaaacaaacc atcgatatca
     2221 tcaccagaag taatgatagt tgtttcatag ttatctcctt tccctttata taattatttt
     2281 tagggtttat tgcgaagctt ggtttcggaa tcgcctatgg ctatcagaaa ctatatgcta
     2341 actaaactat cgattatctt gttctgattc agatggcagc gaagaatttc ttacaaatca
     2401 gcatacctaa ttccaacaat ttactaataa aattattcaa ataagttgtc aagcaagatt
     2461 ttatgtgtat tatatgcaga catctcagtt ttttttagag attgtagggt atagaaaaaa
     2521 cataaggtaa ggaatgaaca ggattcaatt ggaagagggc agaaagtaat gagagaatac
     2581 cactagaaat actataagaa aaactactaa cgcagaaaac gaagttacct ttgttcaaac
     2641 agaataatgg tgtccttgtt gtgtagtggc aactgtgttt tttcgttatg gcttgccttt
     2701 ttagtaaaag caataaagcg tgagaggtgg ttgacgagga catcaacctt ccgatattaa
     2761 ctagccggat taaaacccgt cgttaaaata tatcgtcccg ctgggacttc ttttaggcat
     2821 tctttttatc gccgggttaa aacccgtcgc tcaaatctgt cgtcccgctg ggacttcttt
     2881 taggcattct ttttatcgcc gggttaaaaa cccgtcgtta gtatgtatcg ttcctaacgg
     2941 aactttatag aagaaatcct c
//
LOCUS       DAOSTH010000133         2939 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_30523_length_2939_cov_14.246879, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000133 DAOSTH010000000
VERSION     DAOSTH010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30523_length_2939_cov_14.246879"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            393..635
                     /gene="xseB"
                     /locus_tag="PLB65_08245"
     CDS             393..635
                     /gene="xseB"
                     /locus_tag="PLB65_08245"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOQ78556.1"
                     /translation="MSIEQNKINEMSFEESLKELEKIVERLSTGETSLDELLSLYEAG
                     VAYLKHCQSRLGAAEAKIKILSEQLSPRLDTEGNNG"
     gene            658..1518
                     /locus_tag="PLB65_08250"
     CDS             658..1518
                     /locus_tag="PLB65_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOQ78557.1"
                     /translation="MKEKQELVNIILDRFLPRKDEYPKDLHKAMRYSIFAGGKRLRPY
                     LTMTTFEMFNPEVELITPVAAAIEMIHTYSLIHDDLPDIDDDEFRRGKQSCHTVFGEG
                     IALLAGDALLISAFELVTLAELDAKLKVQLVKELAQEVGIKGLIAGQMVDIESEGKQV
                     DKKTLSYIHENKTARLINICLRFGALAGEAQEEELKILDSYGKLIGMVFQIVDDLLDI
                     EGTQEDLGKSIGKDMDREKATFPSVYGIAESHEMAKEMTEQARQVIAPLGEKALKLDI
                     LAEYLLSRKG"
     gene            1515..1949
                     /gene="dut"
                     /locus_tag="PLB65_08255"
     CDS             1515..1949
                     /gene="dut"
                     /locus_tag="PLB65_08255"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOQ78558.1"
                     /translation="MKIAIQKLTNTAILPQRMTEQSSGFDLFADILESVNLEPYQRKA
                     IPCGIAVEIPIGYEAQIRPRSGLSLGSGLSVLNSPGTIDSDYRGEIKVILINLSDQAM
                     IIEPQMRIAQMVFCPIVIPELCETSILNQSKRQEGGFGHTGK"
     gene            1954..2865
                     /gene="nadA"
                     /locus_tag="PLB65_08260"
     CDS             1954..2865
                     /gene="nadA"
                     /locus_tag="PLB65_08260"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOQ78559.1"
                     /translation="MNKDELIQEIKSLKKDKDALILAHNYQIVEVQEIADYRGDSLQL
                     AMLASELKAPKIVFCGVKFMAETAAILNPESTILLPAMDAGCPMADMIDENQLKAFKQ
                     NYPGSPVVCYVNSSVEVKAESDICCTSSNAVKVINSINTDKPILFVPDRNLGSWAAKQ
                     TGKNVIPWDGYCLVHQWGFTEEDVRSLKSKYPSHKLLVHPECDPTIVKYADFVMSTGG
                     MINWMKENDKAIIATEIGLVEYLQHIHPEKSIIPLSNKAICKNMKKTTLEHIYKALKY
                     DQYAIEVKTDIAQKAKIAIDRMLELSK"
BASE COUNT          987 a          472 c          641 g          839 t
ORIGIN      
        1 aacagggaac gactgacggt gaactagtgc gggggttcaa agttcaaagt gcaggagttc
       61 aaagtgcggg ggtgcaggag tgcttagttt tttagttagc tgcggtggct gattaaatcc
      121 tttttacctc atacataaat aatctgtgga atctgtgtaa tctgtgagag gtatttaaag
      181 ttcaaagtgc aggagttcaa agtgcggggg tgcaggggtg cttagttttt tagttagctg
      241 cgtggctgat taaatcttgt ttatttcata tatagaaaat ctgtgaaatc catgtaatca
      301 gtgagaagta acttaagttt taactttatg attgacaaaa tatctttact atagtataag
      361 gaaacgaata aaaatgaagg agtgaggtga aagtgagtat agaacaaaat aaaatcaatg
      421 agatgagctt tgaagaatcc ttaaaagaac tggaaaaaat tgtggaacgc ttatccactg
      481 gtgaaaccag tttggatgaa ctgctttctt tatatgaagc aggtgtagct tatttaaaac
      541 actgtcaaag taggctcggt gctgctgagg caaaaattaa aatcttaagt gaacagcttt
      601 ccccacgcct ggatacagag ggtaataatg gatagaaaaa cacttcttaa gaaggatatg
      661 aaagaaaagc aggaactggt aaatatcatc ctggatagat ttctgccccg taaagatgaa
      721 tatccgaaag acttgcataa ggcaatgcgc tacagtattt ttgctggtgg aaaacgctta
      781 agaccatatt taacaatgac gactttcgaa atgtttaatc ctgaggtcga acttatcact
      841 cctgttgctg ctgccataga aatgattcat acttattcac tgattcatga tgaccttccc
      901 gatattgatg atgatgaatt tagacgcggt aaacaatcct gccatactgt ttttggggaa
      961 ggcattgctt tattggcagg tgatgcctta ttgataagtg catttgaatt agttacttta
     1021 gcggaactgg atgccaagct gaaagttcaa ttggttaagg aactggcaca agaagttggc
     1081 atcaaaggtt taattgctgg tcagatggta gatatagaat ctgaaggtaa acaggtggat
     1141 aaaaaaaccc tatcctatat tcatgagaat aaaactgcgc gtttaatcaa tatctgtttg
     1201 cgttttggag ctcttgctgg tgaagcacaa gaagaagaac taaagattct ggacagctat
     1261 ggaaaactaa ttggaatggt ttttcagatt gtggatgacc ttttggatat tgaagggacc
     1321 caggaagatc tcggaaaaag cattggtaaa gatatggata gggaaaaagc tacttttcct
     1381 tcggtgtatg gaatagctga atcacatgaa atggcaaagg aaatgacgga acaggcaaga
     1441 caggttattg ctcccttagg tgaaaaagca ctgaaattgg atatcttggc ggaatatcta
     1501 ttaagtagga agggatgaaa atcgctatac aaaaattgac taatactgct attctgcctc
     1561 aaagaatgac ggaacaatct tccggctttg atctttttgc cgatatattg gaatctgtaa
     1621 atttggagcc ctatcagcga aaagcaattc cctgtggtat agctgtcgaa ataccaattg
     1681 gttatgaagc tcaaattaga ccccgtagtg gtttgtccct aggttcaggt ttaagtgttt
     1741 taaacagtcc aggtacaatt gattccgatt accgcggtga aataaaagtg attctgatta
     1801 atttgagcga tcaagcaatg attattgaac cccaaatgcg aatagcacaa atggtattct
     1861 gtccgatagt tattcccgaa ctatgtgaaa cctcaattct taaccaaagt aaaagacagg
     1921 aaggtggttt tggtcacaca ggaaagtgaa aaaatgaata aagatgaatt aattcaagaa
     1981 attaaaagcc tgaagaaaga caaagatgct cttattttgg ctcataacta tcaaatcgtg
     2041 gaagttcaag aaattgctga ctatcgaggt gattcattac agctggcaat gttggcttcg
     2101 gaactgaaag cgccgaaaat tgttttctgt ggtgttaaat ttatggcaga aacagctgca
     2161 attctaaatc cagaatcaac aattctgctt ccggctatgg atgctggatg tcctatggct
     2221 gatatgattg atgaaaatca gctgaaggca tttaaacaaa attatcccgg ctcaccggtt
     2281 gtctgctatg taaattcttc tgtagaagta aaagccgaaa gtgatatctg ctgtacttca
     2341 tccaatgctg taaaagttat caattccata aatactgaca agccaatttt atttgttcca
     2401 gaccgtaatt taggctcctg ggctgccaaa cagacaggaa aaaatgtaat accctgggat
     2461 ggttactgtc ttgtccatca atggggtttc acagaagaag atgttcgatc tctaaaaagt
     2521 aaatatcctt cgcataaatt gctggtgcat ccagaatgtg atcctaccat tgtaaaatat
     2581 gccgattttg tgatgtccac aggaggaatg attaattgga tgaaagaaaa cgataaggcc
     2641 ataattgcta cagaaattgg gctggttgaa tatcttcaac acatacatcc ggaaaaatct
     2701 attattccac tttctaataa ggcgatctgc aaaaatatga agaagacaac tttggaacat
     2761 atatacaagg cgttaaaata cgatcaatac gccattgaag taaaaacgga cattgctcaa
     2821 aaggcaaaaa tagcgatcga tagaatgtta gagctctcca agtaaacaaa tgattacttt
     2881 cttaaaataa ccaaaaggat taagcaatga aaacccgcta tctacttatc ctgtttact
//
LOCUS       DAOSTH010000134         2907 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_30982_length_2907_cov_11.960729, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000134 DAOSTH010000000
VERSION     DAOSTH010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30982_length_2907_cov_11.960729"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..1025
                     /gene="htpX"
                     /locus_tag="PLB65_08265"
     CDS             156..1025
                     /gene="htpX"
                     /locus_tag="PLB65_08265"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HOQ78560.1"
                     /translation="MTFLKRIGIFLLTNFLVMLVLGFIAGLLGLKVDGLWSLLIICAF
                     FGMGGSLISLWLSKPMAKASYRVQIIKPDVAHPKLAFLLSAVENMATERNLKTPEIGI
                     YNSRDANAFATGASQNNALIAFSSALVDRLSEDELAAVAGHEMTHISNGDMVTMTLLM
                     GLVNTFVMFFARILTVLIDNALRDDQGGGLGYFGYMMVIMLLQNVLMLLANIPIAAFS
                     RWREFRADAGAANLAGASAMIEALKKIANAYVPEKRSNAYALAKINNEKRISLFATHP
                     PIELRIKRLQEML"
     gene            1022..2395
                     /gene="rlmD"
                     /locus_tag="PLB65_08270"
     CDS             1022..2395
                     /gene="rlmD"
                     /locus_tag="PLB65_08270"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOQ78561.1"
                     /translation="MKQLIRLKIEKIAMGGFGLAYNDGRAIFVPYTVPEDIVEIVLTH
                     ISKNYAFGKVVNFISYADSRIKVNCDAFAAKNACGGCDWLMLKYTEQLKYKDQLIQDV
                     FSPFIKQDIILPIVASPKANHYRNKVFMPVAENRKTGKISYGIYERFSHRVVEHKSCE
                     NHPPDFDLLANEIMEFCTTTNIKPYNEIQHRGQLRHIGFRCSADTSEILVILVTLGAK
                     LPFTKLLVKKLTADFPHIKGIIQNINREKGNVILGDEEKLLFGSKHFIDTLNNIKFRV
                     NYRSFWQINSGTMANILNIISSQVNSEDVILDTYCGIGTIGISLAAKVKRVVLMEELS
                     AAIDDAKENARMNDIDNVSFVLGKVEDNLASILAKENITKIILDPPRSGVNKNALEAI
                     IQTDLKEIIYLSCSPMTLARDIKILSASDRYTIGYLQPFDMFPNTWHIECLAFLFHKD
                     YNVNREQ"
     gene            2415..2861
                     /locus_tag="PLB65_08275"
     CDS             2415..2861
                     /locus_tag="PLB65_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78562.1"
                     /translation="MKKYLAVLLGMLIMLVLSSCESSDSDTLAQREIRDILYDISYDF
                     NLKDMNGIMEHLHNDYLHRGMNRYNFNNLWLNRMTLYSLLEIEVLYIDLNGNSAIVYS
                     KNKFISNSDTQILNEPEESGDISYFKRDNGVWYIYGDQHMIKSKRH"
BASE COUNT          963 a          473 c          582 g          889 t
ORIGIN      
        1 aactattttc ggtggctaga aaaaaagcgg aggaacggcg tcctccggct acacatataa
       61 aaaaatcctt gccttggacg gcatataaat aatattgtct tagaatgatt taaaatctgc
      121 tttagtagaa aagataagta tacaaggaga atacgatgac attcctaaaa cgaattggaa
      181 tattcctgct aaccaacttt ctggtgatgt tggttttagg ctttatagca ggactgttgg
      241 gtttaaaagt agatggttta tggagtttac taatcatctg tgcttttttt ggaatgggcg
      301 gttcgctaat ttcgctatgg ttaagtaaac ctatggctaa agctagttac cgtgttcaaa
      361 ttataaaacc ggatgtggct catcctaagc tagctttttt actttctgcg gtagagaata
      421 tggctacaga aaggaatttg aaaactccag aaataggtat ttacaatagt agagatgcta
      481 atgcttttgc taccggagcc tctcaaaaca atgccctgat tgctttttct tcagctttag
      541 tagatcgcct ttcggaagat gaacttgctg cagtagccgg tcatgaaatg acccatataa
      601 gtaatggtga tatggttacg atgactttgc tgatgggatt agtgaatacc tttgtgatgt
      661 tttttgcgcg cattttaact gttttaatag ataatgcctt gcgtgatgac cagggtggtg
      721 gattaggata ttttggatat atgatggtga taatgctctt acagaatgtt ttgatgttac
      781 tggcaaatat ccctattgct gctttttcgc gatggcgtga atttagagct gatgctggag
      841 ctgcaaattt agcaggtgct tcagctatga ttgaagccct gaaaaaaatc gctaatgcct
      901 atgttcccga aaaaagaagc aatgcctatg ctctggcaaa aataaataac gagaaacgaa
      961 tttctctatt tgctactcat cctccaatcg aactccggat aaaacgcctt caggaaatgc
     1021 tttgaaacaa cttatacgac tgaaaattga aaaaatagca atgggcggtt ttggtcttgc
     1081 ctacaatgat gggcgggcta tttttgtccc atatacagtt ccggaagata tagtagaaat
     1141 tgttcttacc catatcagca agaattacgc ctttgggaaa gtagttaact ttatcagcta
     1201 tgccgatagt cgcataaagg taaattgtga tgcttttgca gctaagaatg cttgtggagg
     1261 atgcgattgg ttgatgttaa aatatactga acagctgaaa tataaagatc aactaatcca
     1321 agatgtattt agccctttta tcaaacagga tattatttta cctattgtag cttccccaaa
     1381 agctaaccac taccgaaata aggtatttat gcctgtagcc gaaaacagaa aaacgggtaa
     1441 aatcagctat gggatttatg agcgtttttc acatcgggta gttgaacata aaagctgcga
     1501 aaatcatcct cctgattttg atttgctggc aaatgagata atggaatttt gcacaacaac
     1561 aaatatcaag ccttataatg aaattcagca tagaggtcag ctaagacata tcggttttcg
     1621 ttgtagtgca gataccagcg aaattttggt aatattagta actttaggcg ctaaactgcc
     1681 ttttactaaa cttttggtga aaaaacttac tgctgatttt cctcacataa agggaattat
     1741 acaaaacata aatagagaaa aaggtaatgt gattctggga gatgaagaaa agctactttt
     1801 cggcagcaag cactttatcg atacactaaa caatataaaa ttccgggtaa attatcgtag
     1861 tttctggcaa ataaatagtg gcacaatggc caatatcctt aatataataa gcagccaagt
     1921 aaatagtgaa gatgtaattc ttgataccta ctgcggaata gggactatag gtatttcctt
     1981 ggcagctaaa gtaaaaagag ttgttctgat ggaagaactt tctgctgcta ttgatgatgc
     2041 aaaagaaaat gctaggatga atgacattga taatgtttcc tttgtcctcg gtaaagttga
     2101 agataaccta gcttcgatat tagcaaaaga aaacatcact aagataattt tagacccgcc
     2161 tcgcagtggt gttaataaaa atgccttaga agccataatt caaacagatt taaaagagat
     2221 catttatcta agctgctcac caatgacttt ggcaagagat attaaaatct tatcagcaag
     2281 cgacaggtat acaattggat atcttcaacc ctttgatatg ttccctaata cctggcatat
     2341 agaatgcctt gcctttcttt tccataagga ttataatgta aatagggaac aataatcttg
     2401 agagcgagaa tcaaatgaaa aaatatctgg cagttttatt aggaatgttg ataatgctgg
     2461 tattgagtag ttgcgaaagt agtgatagtg atacccttgc tcaacgcgaa attcgagata
     2521 tcctctacga tataagttat gattttaact taaaagatat gaatggaatt atggaacatc
     2581 tccataatga ctatctacat agagggatga accgctataa cttcaataac ctgtggctga
     2641 atagaatgac tctatattca ctgttggaaa tagaagtcct ctatatcgat cttaatggta
     2701 attctgctat tgtttacagt aaaaataaat ttatttccaa ttccgatacg cagattctta
     2761 atgaacctga agaatcgggg gatatatctt atttcaaaag agataacgga gtttggtata
     2821 tctacggcga tcaacatatg ataaaatcaa aaagacacta acgctctata agcaatagag
     2881 atgcaggctt atactatcag tgaatta
//
LOCUS       DAOSTH010000135         2731 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_33695_length_2731_cov_14.925635, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000135 DAOSTH010000000
VERSION     DAOSTH010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33695_length_2731_cov_14.925635"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            466..1656
                     /locus_tag="PLB65_08280"
     CDS             466..1656
                     /locus_tag="PLB65_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HOQ78563.1"
                     /translation="MAQDMNEDRDLYSSELKELLRETLKHSIEGEFSTHSEDPYFYTK
                     KGYPLQTEIDGLPLYPEFIPEVLLTWANLDTTIKRHKEDILVLDLETTGLRSNGIFAF
                     MIGLGYYENEQYIVEQIFLPDPEAELNSFDRLIELIKEKSLLITFNGKTFDVPVLESR
                     LLYHQIWLNLREMEHLDLLHIARRLWKNKLPSCALETLEFYILGQIRDKELDIAGGEI
                     PQTYFNYLINGDPELIRRVFLHNHTDILHTAALFTLICNSISYPPEKGMDIRIDYHSL
                     AMLYQSQNRIDIAKNILLDLLSLGFIDKDILRELGFIYKKEKDYDSAYDCFEIAASLN
                     CPIAMQESCIILEYKYKRYAKALEIAEKLKAYLLSRPIVNLNKVTNTEKRIERLKLKQ
                     NKIK"
     gene            1668..2693
                     /locus_tag="PLB65_08285"
     CDS             1668..2693
                     /locus_tag="PLB65_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HOQ78564.1"
                     /translation="MNSVSFGSDNHSGIHPQVLEAIQKANYGYCPAYGDDPLTIKVLE
                     QIKQLFGGNCDAWFVITGTGANILSLQSVIHSYNAIICPKSAHINTDECGAVQKFTQS
                     RLQPIETTDGKLTPELIAPLLLGNRDQHHSQAKVISISQSTEYGTLYSLQELKALADF
                     AHQNNMFLHIDGARLANAASALNCSLKEMTKDISADVVSFGGTKNGLLCGEAIISFNP
                     EITSDMRFYRKQASQLLSKMRFVSAQFEAYLQDDLYLQNAFCANKMAKLLAKRLQEIK
                     EVTVTQEVEVNSLFVILPEHIIETLQQKYHFYTWNEATNEVRLMCSFNTKEEHIDEFI
                     NDLKTLL"
BASE COUNT          942 a          485 c          491 g          813 t
ORIGIN      
        1 acgcagattt agttttagaa acgaggattt aaggatttta ggattaagag gatttctaat
       61 gtaaagttcc gttaggaacg atacatacta acgacgggtt ttaacccggc gcaaagaaga
      121 acgaataaaa aaagtcccat cgggacgaca gatttgatcg atgggtttta acccggcgaa
      181 aaatacagaa aggtagataa cttcatcaat tcacaacaaa caaactatca agccttcata
      241 gaatatagca gaaacttgat aactcagttg caagctgaac gacaggattt cgttcttcca
      301 ggatatcaaa agtaaaaaag ccccttcctc ctaaaaatcc tgatatccta taatcctcgt
      361 ttctataatg ttggtaatct ctaaaatcta ttcgtgatac ttgacaaaaa ccaaaggcaa
      421 tcaaaaaaag gaatatatta gtatagaaac ttacaggtta ggttaatggc tcaagatatg
      481 aatgaagacc gggatttata ctcttccgaa ttgaaagaac ttctcagaga aacattaaaa
      541 cactctattg agggagaatt ttccactcat agtgaagacc cttatttcta tacaaaaaaa
      601 ggctatcccc tacaaaccga gattgatggt ttacctcttt atccagaatt tattccggag
      661 gttttactaa cctgggcaaa tttagataca actattaaaa gacataaaga agatatccta
      721 gttttggatt tggagactac tggtttacgc agtaatggca tttttgcttt tatgattggt
      781 ttgggttatt atgaaaacga gcagtatatc gtagaacaga tctttctgcc tgatccagaa
      841 gcagaattaa attccttcga tcgcctcatt gaattgataa aggaaaaatc actgctaatt
      901 acattcaatg gtaaaacctt tgatgtgccg gttttagaat ctcggctttt atatcatcaa
      961 atatggctta atttacgaga aatggaacac ttggatttat tacatattgc ccggcgacta
     1021 tggaaaaata aacttccctc ctgtgctttg gaaaccttag aattctatat tctgggtcag
     1081 atcagagata aggaacttga tattgcaggc ggggaaattc cgcagaccta ttttaattac
     1141 ctaattaatg gtgatccaga acttatccgg agagtatttt tgcacaatca tacagatatt
     1201 ttacatacag cagcactgtt cactttaatt tgtaacagca tttcctatcc tcctgaaaaa
     1261 gggatggata taagaattga ttatcattct ttggctatgc tgtaccagag ccaaaatcga
     1321 atagatattg ccaaaaatat acttctggat ttactatcac ttggatttat cgataaagat
     1381 attttaagag aactcggctt catctataaa aaagaaaagg attacgattc tgcctatgat
     1441 tgttttgaaa tagctgcttc tctaaattgc cctatcgcta tgcaagaaag ctgtatcatt
     1501 ctggaatata aatataaacg ctatgcaaaa gcgctcgaaa tagcagaaaa attaaaagct
     1561 tatctgctta gccgtccaat cgttaatctt aataaagtga caaatacaga aaaacgcatc
     1621 gaacggctta aactaaagca aaataagata aaatgaggta aaaataaatg aattcggtaa
     1681 gttttggttc ggacaatcat agtggaattc atccccaagt tttggaagca atccaaaaag
     1741 ctaattatgg ctactgccct gcctatggag atgatcctct aactatcaag gtcttagaac
     1801 aaatcaaaca actttttggg ggaaattgtg atgcctggtt tgtaattaca ggaaccggag
     1861 ctaatatact ttctttgcag tctgttatac attcctacaa tgcaattatc tgtcccaaaa
     1921 gtgctcatat aaatactgat gaatgcgggg cagtgcaaaa atttacgcaa agccgtttgc
     1981 aaccaattga aactaccgat gggaaattga ccccggaatt gattgctccc ttacttttag
     2041 gtaatcggga tcaacatcat tctcaggcaa aagttatttc tatctcccaa agcacagaat
     2101 atggaacttt gtattcattg caggaactga aagcacttgc cgatttcgct catcaaaata
     2161 atatgttttt acatatcgat ggagcgcgtt tggcaaatgc tgcttccgct ttaaactgtt
     2221 ctctgaaaga aatgacaaaa gatatttctg ccgatgttgt tagcttcggg ggaacaaaaa
     2281 atggtttact ttgcggcgaa gcaattatca gttttaatcc agaaataact tccgatatgc
     2341 gtttctatag aaaacaagca agccagcttt taagtaaaat gcgttttgtt tctgctcagt
     2401 ttgaagcata tttacaagat gatttatatc tgcaaaatgc tttctgcgcc aataaaatgg
     2461 caaaactatt agcaaaacgc ttacaggaaa taaaggaagt taccgtaact caggaagtgg
     2521 aagtaaattc cctatttgtt atcttacctg aacatattat tgaaaccctg cagcaaaaat
     2581 accattttta tacttggaat gaggcaacta acgaagtccg actaatgtgc agttttaata
     2641 ctaaagaaga acatatagat gagtttatta acgacctcaa aacgctactg taaatctgat
     2701 aacttctatg tgagtaagtt gtccttgtaa a
//
LOCUS       DAOSTH010000136         2700 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_34218_length_2700_cov_10.450284, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000136 DAOSTH010000000
VERSION     DAOSTH010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34218_length_2700_cov_10.450284"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            152..1021
                     /locus_tag="PLB65_08290"
     CDS             152..1021
                     /locus_tag="PLB65_08290"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOQ78565.1"
                     /translation="MKISYQVGMDGAKHSFTLPRYALILIAIVLLFFITSTIIMLSTA
                     GGNAQRNKHIKHLEAENKELRAKLNFYAATVDSIYKRLDTLQVIINSDSKDYPSLDFE
                     QQLKPNYTFDPALKNRMEELETKLATILVVISDPVTSNYTSTLEASESDFIPSIYPSF
                     GRISDGWGLRVHPISNEIEFHYGIDIANQPGTPIYATAAGTVVTTDYDTSYGKRIIID
                     HGSGYQTLYGHLYSYMVHSGDNVIKGQIIGLMGSSGVSTGPHLHYEVRDNQGKINPTA
                     YLNRIDEPRYAMR"
     gene            996..1676
                     /locus_tag="PLB65_08295"
     CDS             996..1676
                     /locus_tag="PLB65_08295"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020268364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HOQ78566.1"
                     /translation="MNPDMLCAEKVRAYLFTNYAPKNIVSGYQNIEPNDHIIAVDNGL
                     KYVDELGLSPYILIGDQDSLEPALLNKYSQIPCYLHPSEKNDTDTELAINWCINQKCY
                     AEIIICNSLEGRFDHSLAIALNLLKLPYPTMEHMIFPTPYRIESDKQTVFILPDNIEF
                     NNCEGKTLSLISLSHRVIFYNSQNLKYSLNNLVIKQFQSRGISNLIISKQASISKKEG
                     VCLAILSE"
     gene            complement(2440..>2700)
                     /gene="rmuC"
                     /locus_tag="PLB65_08300"
     CDS             complement(2440..>2700)
                     /gene="rmuC"
                     /locus_tag="PLB65_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOQ78567.1"
                     /translation="AAVINSLQVGFRTLAIQKKSSEVWKILASIKQEFGKFGTLLDQT
                     QKKLQEASNTIEKASHRSRQIEKRLRKVQELPVQETEQIMDV"
BASE COUNT          881 a          482 c          492 g          845 t
ORIGIN      
        1 ggactcgtga ttctcagtca cgcaacaggg gttcgattcc cctacgggat gccagaaatc
       61 atgcatattg gtttataaat tgcatatata atttaagtaa tagaaaacaa tcaggtttga
      121 agtggaaaac gaatttatta gtaaaggaaa aatgaaaatc agctatcaag ttggaatgga
      181 tggagctaaa cacagcttca ccttacctcg ctatgcattg attttaatag ctatagtgct
      241 gctttttttt ataacttcca caattataat gctatctaca gcaggtggaa atgcacagcg
      301 gaataaacat atcaagcatt tagaagcaga aaataaagag cttagagcca agctcaattt
      361 ttatgcggcg actgtggatt ctatctataa aagattagat actttacaag ttattataaa
      421 ttcagattct aaggattatc cttctctgga ttttgaacag caattaaaac ctaattatac
      481 ttttgatcct gccctaaaga atcggatgga agaactggaa acaaaactgg ccactatttt
      541 agtggttata tctgatcctg taactagtaa ttatacctca actttagagg ccagtgaatc
      601 agattttatt ccctctatat acccttcttt tggcagaata tcggacggtt ggggtttacg
      661 tgtgcatcca attagcaacg agattgaatt tcattatggg attgatattg ctaatcaacc
      721 aggcactccc atttatgcaa ccgcagctgg aactgttgtt actaccgatt atgataccag
      781 ttatggcaag aggattataa ttgatcacgg aagtggttat caaaccttgt atggtcattt
      841 gtacagttat atggttcatt ccggagataa tgttattaaa ggtcagatta ttggcttgat
      901 gggaagttca ggtgtttcca caggtccaca tttgcattat gaagtccgtg ataaccaagg
      961 taaaataaat ccaacggcat atctaaaccg catagatgaa ccccgatatg ctatgcgctg
     1021 aaaaagttag agcttatcta tttaccaatt acgcaccgaa aaatattgtt tccggctatc
     1081 aaaatattga gcccaatgat catatcatag cggttgataa tggattaaaa tatgtagacg
     1141 aactggggct tagtccttat attcttattg gagatcagga ttcattagaa cctgcattac
     1201 tgaataaata tagccagatt ccctgttatc ttcatccttc cgagaaaaac gatacagata
     1261 cagaactggc gattaattgg tgtataaatc aaaaatgtta cgctgaaata attatctgta
     1321 acagcttgga aggacggttt gaccactcat tagccattgc cttgaacctt ctaaaattgc
     1381 catatccaac aatggaacat atgatatttc caactccata ccgtattgaa agcgataaac
     1441 agacagtttt tatcctgccg gataatatag aattcaacaa ttgcgaaggg aaaacactct
     1501 ctctaatatc actttctcat agggttatat tctataactc ccaaaatctt aaatactcac
     1561 taaataatct ggtaattaaa caatttcaat caagaggaat cagtaatttg attatctcga
     1621 agcaggcaag cattagtaaa aaagaaggtg tctgcttagc tattttaagt gaataataat
     1681 tcgcagctgc atcgataggg gataagcggt ttacaacata tcagcagaat gatagatagt
     1741 tactattgtg tgctaaaatg ttacttgctt tactaacctt attcattatt tcaatgtggg
     1801 ggatctgcga ttctattgta ttacctgggg ttagggaatt aataaaataa gcctgttatc
     1861 aacttattag ctatgataga gttccatttg cttgcttaat tagctaccgt ggcattgccg
     1921 tgtcactact gggcaaatta ccgagaagca gcaccccagt tctaccacag ataattaaca
     1981 aaggacttac tccgttgatt acatatatac cttcctagat taagaggatt tatttataag
     2041 ccaccgagac caccgaaaat aactcacgca gattacacag atatagtttt agaaacaagg
     2101 attttaggat ttgagaagat tatattgtat aactgacaat tagtcgactt taagtcaaaa
     2161 tcggcctttt ctatgagatt aatagtgact tttttcaaag ttagggaaaa agaatgaaat
     2221 tatgtggaaa atcacaaaca aaaccgattt taactcaaag gccgacacaa tgaaagcgat
     2281 ggtggcgtaa gcccctcgaa acctaattgc caggtgctta aagtctattt tcaatcaatt
     2341 aggcattatt actttctgta tggttttatg cttgctcagg aaggataatt acgaagcagg
     2401 aaaattagcg ctcggcattt atcacacaca taaatgatat tatacatcca taatttgttc
     2461 tgtttcttga acaggcaatt cctgaacctt cctcaggcgt ttttcaattt ggcgactccg
     2521 atgacttgct ttttcaattg tattagatgc ttcttgtagt ttcttttggg tttgatccag
     2581 taaagttcca aattttccaa attcctgttt gatagaggca aggattttcc atacctcaga
     2641 gctttttttc tgaatagcca gagttcgaaa acctacttgt aaactattaa taacagcggc
//
LOCUS       DAOSTH010000137         2618 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_35693_length_2618_cov_17.101053, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000137 DAOSTH010000000
VERSION     DAOSTH010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35693_length_2618_cov_17.101053"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            126..2420
                     /locus_tag="PLB65_08305"
     CDS             126..2420
                     /locus_tag="PLB65_08305"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HOQ78568.1"
                     /translation="MKLAQKIILTLLLFLPVFIAAELPSCYYTYDQIYTLLQNHQQAH
                     PDIAKIYTIGHSQQDNIPIYAIRISDNVETDESEPALLFVGQVHAEEVLGVQTTMANI
                     AEILTYRYQQPYSQWISQLDLWFIPTLNPEGHNVVTANLDVSYRKNKRDNNNNGIFDF
                     NPVTGGDIDGVDINRNFAFNWVHGDTLYCPVGDEQYDYYRGPASMSESEIIGFQQLCE
                     QYKFIYAVCWHSSRRGGLSEKAYYPFNWKNVRPSPDLNFTASIATGFASQILKQDGTG
                     TYQAYPNLSRQGAFHDWLYQQYGTIAFIVECGTRDLQPDSTVMVGTVQRCSNGVRWLL
                     NRALMFSTNVSSSSMLTGTVSSGGVPLEAEIIIQQHSAPWFRPRTSFASSGKFYRPLA
                     SGSYTVIARKKGYYDKVINNQTVNNGSWTNIQINLEPKPAATLRIGVRCDAEPISARV
                     IIGDVFPDTLFVNGFANLNTYAGEYPIQIYAEGYTPYLGTINLIEGHNHKEYNLSTST
                     TIFTEDWENGSSAWEINGPWKVQNQLAASGYAITDSIGGNGFYDMNCDVWIKTIQSIQ
                     IPATNNTWLIFDSHLYTEWNYDPCRVEVSTDGTNWQEIWTKSGQWDTFRKEFVSLNAF
                     AGQSIFLRFRLTDSSTDVDLTDPGWTLDNILITSGTATANNDPNIPVTPLTLLYKNYP
                     NPFNPETTLSFTLAKSGRTILKIYNQKGQLVKTLIDADLPSGDYQYVWNGKDNKGISV
                     ASGVYLYSLIAEGETHLRKMLLLK"
BASE COUNT          757 a          526 c          534 g          801 t
ORIGIN      
        1 gcactttgaa ctttgcactc ctgcactttg aactttcatt gtttcgcatc tttattttct
       61 tcttgtttgg agcataaatt gcattattat ttgctaagag agaaaaatca aggagaatcc
      121 aaacgatgaa attagcacaa aagataatat taaccctgtt gctttttttg cctgttttta
      181 tagcagcaga actaccttct tgttattata cctatgatca aatatatact ttattacaga
      241 atcatcagca agctcatcct gatatagcca agatatatac aattggccat tctcaacagg
      301 ataacatccc aatttatgct atccgaattt cagataatgt agaaactgat gaatctgaac
      361 ctgctttact ttttgttggt caggttcatg cggaagaagt tttgggagtg caaactacga
      421 tggctaatat tgctgaaatt ttaacttacc gctatcagca accttattcg caatggattt
      481 ctcagcttga cttgtggttt attcctactc tcaatccgga agggcataat gtagttacgg
      541 caaatttgga tgtttcttac cgtaaaaata aaagggataa caataacaac ggcatttttg
      601 attttaatcc ggtaaccggt ggggatatag acggtgtaga tataaaccgc aattttgcct
      661 ttaattgggt gcatggtgat actctttatt gtccggtggg agatgaacaa tatgattatt
      721 accgcggtcc tgcatcaatg agtgaaagtg aaataattgg ttttcagcaa ttatgtgagc
      781 agtacaaatt tatttatgcg gtctgctggc attcttcgcg cagaggtggt ttatcggaaa
      841 aggcctatta tccttttaac tggaaaaatg ttcgtcccag tcccgaccta aatttcactg
      901 cctctattgc tacaggtttt gcttctcaaa ttctcaaaca ggatggcacg ggaacttatc
      961 aagcatatcc taatttgagt cgtcagggtg cctttcacga ttggctttat cagcaatatg
     1021 gcaccatcgc tttcatagtg gaatgcggaa ccagagatct tcaaccggat tcaacagtta
     1081 tggttggaac cgttcagcgt tgtagcaatg gagtccgctg gttactaaac agagctttaa
     1141 tgttcagcac caatgtttca tcatcatcaa tgctaactgg aacagtttct tctggaggag
     1201 ttcctttgga agcagaaatt attattcaac aacacagtgc gccctggttt cgtccccgaa
     1261 cttcttttgc ttccagtgga aaattttatc gtcccttggc aagcggctct tacactgtca
     1321 ttgctcgtaa aaagggttat tacgataagg ttattaataa tcagacagtt aataacggga
     1381 gctggacaaa tatccagata aatctggaac ccaaaccagc agctacttta agaatagggg
     1441 ttcgctgtga tgcagagccc atttctgccc gagtcataat cggtgatgtt tttcctgata
     1501 ctctttttgt taacggtttt gccaatctga atacttacgc aggagaatat cctatccaga
     1561 tttatgctga aggctacact ccctatctgg gaacaattaa tcttattgaa gggcataatc
     1621 ataaagagta taacctgagt acttccacta cgattttcac tgaggactgg gagaacggaa
     1681 gttctgcctg ggaaattaac ggaccctgga aagtgcaaaa tcaacttgct gcttctggat
     1741 atgccatcac cgatagcatt ggaggtaacg gattttacga tatgaattgc gatgtgtgga
     1801 ttaaaactat tcagtcaatt cagattccag ctaccaataa cacctggcta attttcgatt
     1861 ctcatcttta tacagaatgg aattatgacc cctgccgagt tgaggtttcc accgatggaa
     1921 ctaattggca ggaaatttgg actaaatcgg ggcagtggga tacttttaga aaggaatttg
     1981 tttccctgaa tgcctttgct ggacagtcca tttttttaag gttccgcctt accgattcct
     2041 ccacggatgt ggatttaacc gatccgggat ggacattgga taatattcta atcaccagcg
     2101 gaaccgctac cgctaataac gatcccaata ttccagttac gccgcttact ttgctatata
     2161 aaaattatcc caaccccttt aatcccgaaa ctacccttag tttcaccttg gcaaaaagcg
     2221 gaagaaccat tttaaaaatc tacaatcaaa aaggccaatt ggtaaagacc ctcatagatg
     2281 ccgatttacc ctcgggtgat tatcaatatg tttggaatgg aaaagataat aaaggtattt
     2341 ctgtggccag cggagtgtat ctctattctc tgatagcaga aggtgaaacg catctccgaa
     2401 aaatgctgct tttgaaataa cagccctccc tttagaacgc tataatcatc aattgcccta
     2461 tcgaggatgt tgctaaaatc ggttcctatg tatagggctg ataattattg cttgcttaat
     2521 tagctgtggt ggagataccg tggtgcctct gcgggaaatt ccaaggtgcg ctgccacagc
     2581 agcactgaag atagtaagga ggaaacagaa ttgtagag
//
LOCUS       DAOSTH010000138         2614 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_35770_length_2614_cov_12.096522, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000138 DAOSTH010000000
VERSION     DAOSTH010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35770_length_2614_cov_12.096522"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            378..1916
                     /locus_tag="PLB65_08310"
     CDS             378..1916
                     /locus_tag="PLB65_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78569.1"
                     /translation="MRTNKVTILIVLIWLVTIALYAQQPHLEVTIKDSLTIPHTYDYV
                     FQLPNGDLQFYKLHYGTSTLQISSFQYNIQINQTTPLVDIGTVSGLEGITNYGMYWKW
                     RFNRLYLVHELTSGIAVIMLNQGLLTSRVISDVSVDQDFNLRTMTDIVAVDAIVIAAT
                     DSLVYYNIIDGNSVTLLQGNAYYCVIDQYPVVLSLPDTHFLYAKDGVFGVLEEVWFIY
                     DSAGNIITTQVSTDELLLNSYFEKGSGFEPKSIFGKWYVRYPGLPMSNGCLECSFPEP
                     DSLHYYFFEPPSAIEEWLEDISQFGDNGILRLYFDDLTELLFFYYNRSPLEQYPDVIY
                     SFCIGPYTPTITPVSEDITTISTSLPSSIAISALWTYDFPVVHQFYFPVSTNVSYSCK
                     AFSYNKTQFIIKDNKIYSFDVSVSCSNDEDLAVINNCEIQIFPNPIISDQQLTIRSNS
                     KNNNVLDIYNLKGQKVKTLYMKDIREIVWDCTDDQGNKLAAGLYIINQRNRGTKPLKF
                     MLIK"
BASE COUNT          872 a          437 c          496 g          809 t
ORIGIN      
        1 ctgatcaaag cccggaaggt gatacaatca tagcgagggt ggcgtaagcc cctcgagaat
       61 tgaaattggc aagatgaaga taatgcattt gttagtatat actaacatag attgtgagaa
      121 atgaggataa tatgaagtgt cctcaactaa gctgagaaac aatgagttgg aatggacacc
      181 aacatttggg actcaactcc caattttttt tgaaaaagaa cttgacttaa attgataaga
      241 tgaaattaat acaatttgtc gcttaagctt ttatatattg tgtgtaatga agataataaa
      301 ttgtcctcaa ctaacctgag aaacaatgag ttggaatgga caccaacatt ttggactcaa
      361 caaatctgga ggattaaatg agaacaaaca aagttactat attgatagta ctaatttggt
      421 tggtcactat cgcattgtat gcacaacaac cgcaccttga ggttacgata aaagattctt
      481 tgactattcc acacacatac gattatgtgt ttcaacttcc taatggcgat ctccagtttt
      541 ataaactaca ctatggaact tccaccctgc agattagttc ctttcaatat aatattcaga
      601 tcaatcaaac tacacccctg gtggatattg gcaccgtttc agggcttgag gggattacga
      661 attatgggat gtattggaaa tggaggttta ataggttata tcttgttcat gaactgactt
      721 ctggaattgc ggttattatg ctaaatcaag gccttctaac gagcagagta atttctgatg
      781 tttccgtgga ccaggatttt aatctacgca ctatgactga tattgttgcc gtagatgcta
      841 ttgttattgc agcaacagat agcttagttt attataatat aatagacggc aacagtgtaa
      901 cattactcca gggcaatgcc tattactgtg taattgatca gtaccctgtg gtgctttcat
      961 tgcctgatac ccattttcta tacgcaaaag atggagtttt tggagtactt gaagaagtgt
     1021 ggtttattta tgattcggct ggaaatatca tcacaaccca agttagcact gatgagttac
     1081 tattaaatag ctattttgaa aagggatctg ggtttgagcc caaatcaatc ttcggtaaat
     1141 ggtatgttcg ttatcccggg cttccaatgt cgaatggttg tcttgaatgc tcttttcccg
     1201 aaccggattc tttgcattat tatttctttg agccaccctc agcaatagag gagtggcttg
     1261 aggatatctc gcaatttgga gataatggaa tcctaagatt gtattttgat gatttgactg
     1321 agctgctttt cttttactat aacaggagcc cattagagca atatcctgat gtaatctatt
     1381 ctttctgtat tggtccctac actcccacta tcacaccagt ttcggaagac attactacta
     1441 tttctactag ccttcccagt tctatagcaa tatcggcatt atggacttat gactttccag
     1501 ttgtgcatca gttttatttt ccagtaagca ctaatgtgtc atatagctgt aaagcttttt
     1561 cttacaacaa gacccagttt atcataaaag acaacaaaat ctatagtttt gatgttagtg
     1621 tatcctgttc caatgacgaa gatttagcag ttataaataa ctgcgagata cagatatttc
     1681 ccaatccaat aatatcagat cagcaactta caatacgatc taactctaag aataataatg
     1741 ttttagatat ctataatctt aaggggcaaa aggtcaaaac actttatatg aaagacatta
     1801 gagagattgt ttgggattgt acagatgatc aaggtaataa gcttgccgca ggtttatata
     1861 taataaatca acgtaacagg gggacaaaac ccctgaaatt tatgctgata aaatagcata
     1921 aagagatatt ataagatcat tgcacagaaa agtggacaaa atatggtctt aaataatttt
     1981 tgacaaaaca aatggggata ttaaattagt tccataatta ttaaggagct tattatggaa
     2041 aagcaaacta aagccgatca agagattata aaccgcttaa gctaatcaaa agcactttct
     2101 taaagaaata gatgtgataa tggactggac taaaatcaac aaagttctct ccaaggtaga
     2161 aattggacgc gaatctgtag ctggcaaaaa ctaacaccga tgcttgtttt gacttacaat
     2221 tttgccacta ccctctcagt aaccacatcc ctaaaattgc acacattttc aaacgaatta
     2281 taattggtcc cctaacttca aaattgtgag ttaaaacaag aaaaaagcac attgccaacc
     2341 gagttgtagt tttggaaatt tgatgaaata ttttggttgg gataggtcta tctatatggg
     2401 attaaagaaa actgcagatt atttggtgat gggggcaatt gcctttaatt tgaaacgcag
     2461 tataaaaatt ttacgagcat agtatagaag gggtttgccc agattatggg ataagggtaa
     2521 aaaatgaaca aatttgaggg tttggggaag gcataatggt aaaaaacaga taaaaaagac
     2581 ctattctttg attatttctc ccaatcagcc aaaa
//
LOCUS       DAOSTH010000139         2573 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_36523_length_2573_cov_11.530977, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000139 DAOSTH010000000
VERSION     DAOSTH010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36523_length_2573_cov_11.530977"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /gene="cas1"
                     /locus_tag="PLB65_08315"
     CDS             <1..1025
                     /gene="cas1"
                     /locus_tag="PLB65_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOQ78570.1"
                     /translation="RKKDEMFELSIEDRKTKLSPEKISSIVISNAAIITTDAIQLAMD
                     YNIDIVFLDKYGNPYGRIWFPKIGSTVLIRRRQLEMLSDSVGLQFTKDWVAIKIMNQY
                     RFVQRLLSKRDCDKSIFQNRMQNMQEAAICIMQAEGNLEELSGSFMGWEGSASKNYFS
                     ILAELIPDAYKFAGRSSRPAKDAFNAMLNYGYGMLYSKVERALIIAGLDPYVGILHSD
                     NYNKKSFVFDFIEPYRILVDEPVFYIFSRHKFSPEFIEPVHQGVLLSAAGKKFLAPLL
                     LEHFDEIIRYRNKNRKRIEMLKTDAHAFANYLIDKRENYLETSINRKLQNFLNSNFED
                     NGGEEC"
     gene            1019..1309
                     /gene="cas2"
                     /locus_tag="PLB65_08320"
     CDS             1019..1309
                     /gene="cas2"
                     /locus_tag="PLB65_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOQ78571.1"
                     /translation="MLTWVMYDIVKDKIRNKVSKCCEQAGIYRVQYSVFLGEMSRTKC
                     KELSCQIEDLINPEIDRVYIFPMCNEDFKSCQLLGQAFDPELITNEIKAFFL"
     gene            1306..1896
                     /gene="cas4"
                     /locus_tag="PLB65_08325"
     CDS             1306..1896
                     /gene="cas4"
                     /locus_tag="PLB65_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HOQ78572.1"
                     /translation="MSDFQFITPSEVMEYLFCPRFVYYMNTMKIEQHEHRRTLVNKGR
                     DIHKLKMVQNKDYLRKKAGAIDKLTDVYLSSEKLKLVGKVDEVLFLVDGSAAPLDYKY
                     AFWENKIYKTLKYQQILYSLLIMENFQVPVYKAFIVYTRSQNHLEELAITNRMLDKAK
                     LILDDIFKIINMEIYPKPAKAKRKCLDCTYRNLCSA"
     gene            2337..>2573
                     /locus_tag="PLB65_08330"
     CDS             2337..>2573
                     /locus_tag="PLB65_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78573.1"
                     /translation="MTKEGFISYYPLKQGLKQYTFSEGNSSWKIFISYYPLKQGLKLH
                     ARHQRNSIGCIYILLSIKTRIETLHYLAVLGFGIE"
BASE COUNT          823 a          412 c          491 g          847 t
ORIGIN      
        1 tacgcaaaaa agatgaaatg tttgaattga gcattgagga taggaaaacc aagctctctc
       61 cggaaaagat atcttccatt gttatttcca atgctgctat tataactacg gatgctattc
      121 aattagcgat ggattataat atagacatcg tttttctgga taaatatggc aatccttatg
      181 gcagaatttg gtttccgaag attggcagca ctgttttaat tcggagaagg cagctggaaa
      241 tgttatctga tagtgtaggt ttacaattta ctaaagattg ggttgccatt aaaataatga
      301 atcagtatcg ttttgtgcag cgtttacttt ccaagcggga ttgtgataaa agtatctttc
      361 aaaatcggat gcaaaatatg caggaagcag ctatttgtat aatgcaggca gaaggtaatt
      421 tagaagaact ttcgggttct tttatgggtt gggaaggaag tgcgtccaaa aattactttt
      481 ctattctggc agagctcatt ccagatgctt ataaatttgc gggcagaagt tcgcgtcctg
      541 ccaaagatgc tttcaatgct atgttgaatt atggctatgg tatgctttat tccaaagtgg
      601 agcgagcttt aattattgct ggtcttgatc cctatgttgg tattttacac agtgataact
      661 ataacaagaa gtcctttgtc tttgatttta tagagccgta tcgtattttg gtggatgagc
      721 cggtattcta tattttttcc cggcataaat tttctcctga gtttatcgaa ccggttcatc
      781 aaggtgtttt acttagcgct gcaggcaaaa aattcctggc tcctttgttg ttagaacatt
      841 ttgatgagat tattcggtat cgcaataaaa accgtaaacg cattgaaatg ctaaaaactg
      901 atgctcacgc ctttgccaat tacttaattg ataaaaggga aaactatctg gaaacatcta
      961 taaacaggaa attacagaac ttcctaaata gcaattttga ggataatgga ggtgaagaat
     1021 gttaacttgg gttatgtatg atatcgttaa agacaaaatt cgaaataaag tttccaaatg
     1081 ctgtgaacaa gccggaattt atagagttca atactctgtt tttttgggag aaatgagccg
     1141 cactaaatgc aaagaactaa gctgtcagat agaggactta atcaatcccg aaattgatcg
     1201 agtttatatt tttccgatgt gcaatgagga ttttaaaagt tgtcaattgc ttgggcaagc
     1261 atttgatcct gaactcatta cgaatgaaat aaaggcattt ttcctatgag cgattttcag
     1321 tttatcactc cttctgaagt gatggaatat ctcttctgtc caagatttgt ttattatatg
     1381 aacacaatga aaattgaaca acacgaacac agaagaactc tcgtaaataa aggcagagat
     1441 attcataaac tgaaaatggt acaaaataag gattatttac gcaaaaaagc cggtgccatt
     1501 gataaactaa cggatgtata tctttcttcc gaaaaactta aactggtagg aaaggtcgat
     1561 gaggttcttt ttcttgtcga cggcagtgcc gcgcctctgg attataaata tgccttttgg
     1621 gaaaacaaga tctataaaac ccttaaatat caacagattc tctattccct tcttataatg
     1681 gaaaattttc aggttcctgt ttataaagca tttattgtct atacgagatc tcaaaaccac
     1741 cttgaagaat tggctattac caatagaatg ctggataaag ctaaattgat ccttgacgat
     1801 attttcaaga tcattaatat ggaaatatac cctaaaccag caaaggcaaa aagaaaatgc
     1861 ttggactgca cctatcggaa tttgtgttct gcctgactaa atgagagaaa aacagttttt
     1921 tgcttttctg aataaactct ttattatata gtgtgtaaca ggatattaca agacacttga
     1981 gattcctgat ttttatcgga tatgataaca aatatagcta gttactatat tatagcttgt
     2041 cattccgagc ttgatgcaga atctttatta ttcctgctat tgtcaggact ataagtgttt
     2101 ttttttaaga gagatagtaa taattgattt atccttgctg atatatatgc tttgtacttg
     2161 aatcctgaga agaaattatt gtttttttag cgtgtttttg ctcttttata tgtgtataaa
     2221 gtgtttgtgc ctgcccgaaa gaactttttg gctctttcga gaactggttt tcctgacgaa
     2281 agagtgattt taagattccg aaaaaccagt ttaatactat tatttgtgtg gcacttatga
     2341 caaaagaagg atttatatcc tactatccat taaaacaagg attgaaacaa tatactttta
     2401 gcgaagggaa cagttcctgg aaaatattta tatcctacta tccattaaaa caaggattga
     2461 aactacatgc tcgacaccag aggaacagca tcggctgcat ttatatccta ctatccatta
     2521 aaacaaggat tgaaaccctt cactatcttg ccgttctcgg attcgggata gaa
//
LOCUS       DAOSTH010000140         2497 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_38035_length_2497_cov_10.893120, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000140 DAOSTH010000000
VERSION     DAOSTH010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38035_length_2497_cov_10.893120"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(261..2024)
                     /locus_tag="PLB65_08335"
     CDS             complement(261..2024)
                     /locus_tag="PLB65_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78574.1"
                     /translation="MKKLFALILGLMLATGILFAQPWVYDFGTETRSFPSTGYTNSTF
                     LPQPTSGEDYVKVGSGGGIYLNNPGLDDFGSDSELRIVAASSGNMNKFSVYDYSSFVR
                     FYIKFDIRFGDSVGGNTANSGKFYFVQGHGNTFSSEKNYDPAQSFTVLSFEFGDNGSI
                     TTKHRSDTSIINLLTSSPITQGTNNTIEIWGNNSSVNSTFTYYRNGISYTLRQNMQDI
                     WINGIRNSSIPKASIKDGIAIDSFMFDGEGSTNNVANCFIDNIVYSYDLYSSPSTQAT
                     INSSPISSYDSATLNWSNGNGSKRVVLLSTHSDIIPPQDGTDPEANSVFNSRGQQVVY
                     NGTGNSVTVTGLSPSTTYYAKVFEVQGSGLTTMYNTTGTSTQFITQEETLPVELTNFN
                     ADLDYRNRIRLQWVTQSEANLLGYYIYRNCADNFSSAKVVSPLIGATNTSQQQIYAFT
                     DETINTGGIYYYWLQSIDYNASENIFGPAIYKFELPETGVGVEIPLQEGIRSVYPNPC
                     NPATTINYELEGAETVQLSIYNLKGQRVVDKTFEHSGKGSYNYFWNGCDERGKECATG
                     IYNVIMRCGERIYIRKLTLLK"
BASE COUNT          810 a          469 c          395 g          823 t
ORIGIN      
        1 gcactttgaa ctcctgcact ttgaacttcc tatttagaat cataataagg agatatcacc
       61 attaacatta tcctccaatt cttaatctat tttatctgtg aaatctgtgc aatctgtgag
      121 agctttcatc ctttaaattt tttacttttt tatctgtgaa atctgtgcaa tctgtgagag
      181 ctttcatccc ttaaattctt tactttttta tctgtgaaat ctgtgcaatc tgtgagaggt
      241 aaaaaaatcc gcattaggtt ctatttcagc aatgtcaatt ttctaatgta aatcctttct
      301 ccacatctca ttataacatt atagatccct gttgcacatt cctttcctct ttcatcacaa
      361 ccgttccaaa aataattata actgcctttc ccgctatgtt caaatgtttt atctaccacc
      421 ctctgccctt taaggttata aattgataat tgaacagttt cggcgccttc caattcataa
      481 ttgatagttg tagcaggatt gcaaggattg ggataaacac tccgaatccc ttcttgcaaa
      541 gggatttcta ctcctacacc tgtttcagga agttcaaatt tatatatcgc tggaccgaat
      601 atattttcgc tagcattata atcaatactc tgcagccagt aataataaat acctccggta
      661 ttaattgttt catcagtaaa agcataaatc tgttgctgcg aagtattagt ggctccaatc
      721 aaaggagaaa caacttttgc agaggaaaaa ttgtccgcgc aattcctgta aatataatac
      781 cccaaaagat tcgcttccga ttgagtaacc cattgcaacc taatcctatt ccgataatcc
      841 aaatcagcgt tgaaattcgt caactctaca ggtagagttt cttcttgagt tataaattga
      901 gttgaagttc cagtagtatt gtacattgtg gttaagccag aaccttgaac ctcaaaaacc
      961 tttgcataat atgttgtaga aggtgaaagt ccagttactg ttactgaatt ccctgtccca
     1021 ttataaacaa cttgctgacc tctgctatta aaaacagaat tagcttcagg gtcagttcca
     1081 tcctggggag gtataatatc gctatgtgtg ctaagtaaaa caactctttt actcccgttg
     1141 ccattactcc aattaagtgt agcagaatcg tacgaactta ttgggctact attaattgta
     1201 gcctgagtgg aaggacttga gtatagatca taagaataaa ctatgttatc tataaagcaa
     1261 ttagccacat tgttagtact accttctcca tcaaacataa aggaatctat ggcaatacca
     1321 tcttttatag atgctttagg tatactgctg tttcgaatac cattaatcca aatatcctgc
     1381 atattttgtc taagtgtgta agaaattcca tttctataat aagtaaatgt tgaattaact
     1441 gatgaattgt ttccccatat ttctatggtg ttattagtac cttgggttat tggtgatgaa
     1501 gtaagaaggt taataattga agtatcagac ctatgttttg ttgttataga accattatcg
     1561 ccaaactcaa atgatagtac agtaaaagat tgtgctggat catagttttt ctcacttgaa
     1621 aaagtattgc catggccttg cacaaaataa aatttccctg aatttgcagt atttccacct
     1681 actgaatcac caaacctaat atcaaatttt atatagaaac gaacaaaact actataatca
     1741 tataccgaaa acttattcat attgcctgaa cttgcagcta ctattcttaa ttctgaatca
     1801 gaaccaaagt cgtcaaggcc aggattattc aaatatattc ctcctccact accaactttt
     1861 acataatctt caccagatgt aggttgaggt agaaaagtag aattagtata acctgttgag
     1921 ggaaaagatc tggtttcagt tccaaaatca taaacccagg gttgagcaaa gagaataccc
     1981 gttgccaaca taagccccaa aataagagca aatagctttt tcattgtttt cctctatgct
     2041 gttactatat ttacgcaata gtaagcatgt ttttaataaa ttcatttatc ttttcagaga
     2101 ggatgctgtc aagataaact ttccccatat aagcaaaagc gggataacta tcccgctttg
     2161 caataggtta tgagaccaaa ctttctgctc gtctctatga gaaagaaaca aaaagtttag
     2221 aattttatgc tgaaaaaaag atggttttag agaataataa tgccgatttg gctatgaaac
     2281 actttttttt actcttcgta attactcccc cgcgctaaca aaagatagat ccccctttac
     2341 aaattaagaa acaaggattt aaggattagg agtgttatta tattagctta agaaacaagg
     2401 atttaaggat tttaggatta agaggatttt ttattattag ccaccgaaga caccgaaaaa
     2461 atgtaagcac gcagtagaaa caaggattta aggattt
//
LOCUS       DAOSTH010000141         2405 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_40078_length_2405_cov_14.605106, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000141 DAOSTH010000000
VERSION     DAOSTH010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40078_length_2405_cov_14.605106"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..1214
                     /locus_tag="PLB65_08340"
     CDS             165..1214
                     /locus_tag="PLB65_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78575.1"
                     /translation="MRKIQIVLILITALIFSACCHNKAKPVTSETENLNYTGSKLKSN
                     YIWGGAMNLAWNELIENFTGEPIELATKDENALKTLHCLNNPVFTKDDIDEASYYVKS
                     GYGQKTVDLINQECRAKFPDKSISDLQLKLADKDIISYAYFLKQIEYEIAFKKQAVNF
                     LDKQVKGFTSDPESYTNVYIVNYENDDKFLLALKLKDNQDQIFLAKGYPMDKPDEVLK
                     MLKTKAPANEKADFIPGTVMNRRDIFQAPVLHLNYERQYKEMLNKKIKNKKLSGYQIA
                     LMQEIIKFDMDEKGARVENEAIIGMITSANPNASKPKNLILDKPYWVIMKRANSNNPY
                     FILGVGDTELMNAAD"
     gene            1238..2404
                     /locus_tag="PLB65_08345"
     CDS             1238..2404
                     /locus_tag="PLB65_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HOQ78576.1"
                     /translation="MPDPVINAFENALKELNSFSELKKDLRNRNLSNPLKILAIGKSA
                     YPMANVCCDILTTSGIEYSGYLLTKYGNIGAEIPNLVIKEAGHPVPDNNTLQNSQEIL
                     NWLQHIDEKEDLIILLSGGGSSLFEVLENGHTLEDLIALNKTLLQSGVGIKEINMERA
                     KISKVKAGKALNYINAQNIYCYALSDVQNNEPETIASGCFYPTNAIKIDENSYQAILN
                     NNKQQLFYTIVGDNYSLRKQLANFIPPPVYIQPIYFYQNVDIVADYLANFAFSPNQKG
                     LYIFGGEAPVKVTGNGIGGRCTHLALLMAKKIAGKKQITFYALASDGNDNLDNVSGAW
                     VDQNTANKLQNKGIDISCVIKNCDSYTALKTVESIIPAWVQPLNLNDIYLLEIK"
BASE COUNT          862 a          421 c          438 g          684 t
ORIGIN      
        1 ttaaagtcga ctaattgtca gttaaaaaaa tcaatcctct taatcctaaa atccttaaat
       61 cctcgtttct aaaatcaaat ctgtgagaac caagagctaa acatttgtat tgacacaatc
      121 tcctcggaat ttatcctgcc aaaaagaata gaaagaggta tccaatgcgg aaaattcaaa
      181 ttgtattaat cttaataaca gctcttatat tttcggcttg ctgtcataat aaagccaaac
      241 cggttacatc tgaaactgaa aacttgaatt atacaggcag caaactgaaa agcaattata
      301 tttggggcgg agctatgaat cttgcctgga atgaattaat agagaatttt acaggagaac
      361 ccatagaact tgccaccaaa gatgaaaatg cattaaagac attacattgt ttaaataatc
      421 ccgtttttac caaagacgat atagatgaag caagttatta tgtcaaaagc ggatatgggc
      481 aaaaaaccgt tgatctgata aatcaagaat gccgggcaaa atttcctgat aagagtatat
      541 cggacttaca gttgaaattg gctgataaag atataatctc ctatgcctat ttccttaaac
      601 agattgagta cgaaattgct tttaagaagc aggcagttaa ttttttggat aaacaagtga
      661 agggctttac ttctgatccc gaaagctata ctaatgttta tattgtgaat tacgaaaatg
      721 acgataaatt cctgctcgcc ttgaaattaa aagataacca agatcagatt tttttggcaa
      781 agggctatcc tatggataaa cccgatgaag ttctaaaaat gctgaaaacc aaagctcctg
      841 ctaatgagaa agcggatttt attcccggaa ccgtaatgaa ccgaagagat atatttcaag
      901 caccggtttt acacctaaat tatgaacgcc aatataaaga aatgttgaat aagaaaatca
      961 agaacaaaaa gctttctggt taccaaatcg ctctgatgca agaaattatc aaatttgata
     1021 tggatgagaa aggtgctcgc gtagaaaatg aagctatcat cggaatgatc acttccgcca
     1081 atcccaatgc ctctaagccc aaaaatctga ttttggataa accttattgg gtaataatga
     1141 aaagggcaaa ttccaataac ccctatttta ttctgggagt tggtgataca gaattaatga
     1201 acgcagccga ctgaacgaga tatgtaaagc taaatatatg ccagatccgg tgatcaacgc
     1261 ctttgaaaat gccctgaaag aactcaattc cttttcagaa ctaaaaaagg acttaaggaa
     1321 tagaaattta tccaatcctc ttaaaatttt agccatcgga aaaagcgctt atccgatggc
     1381 taatgtttgt tgcgatatcc ttactacaag cggaatagaa tactccggct atttgctaac
     1441 caaatatgga aatattggtg ctgaaatacc aaatttagtg ataaaagaag ctggtcatcc
     1501 tgttcctgat aacaatactc ttcaaaatag ccaggaaatt cttaattggc tgcaacatat
     1561 tgatgaaaaa gaagatttga ttattttgct tagcggaggt ggaagttctt tatttgaagt
     1621 gctggaaaat ggacatactt tggaagacct gattgctctg aataagacat tgctacaaag
     1681 cggagtagga atcaaagaga taaatatgga gcgcgccaaa atatccaaag tgaaagccgg
     1741 aaaagcacta aactatataa atgcacaaaa tatatactgt tatgccctat ccgatgtgca
     1801 aaataatgag ccggaaacta ttgcttcggg ctgtttctat ccaacaaatg ccataaaaat
     1861 tgatgaaaat agttaccagg caattttgaa taataataaa caacagcttt tctatactat
     1921 tgtgggagat aactattctc tgcgtaagca gttagctaat tttattcctc cacccgtata
     1981 tattcagccc atatattttt atcagaatgt ggatatagtg gcagattacc ttgctaattt
     2041 tgctttttcc ccaaaccaaa aaggtctgta tatctttggt ggagaagcac ctgtaaaagt
     2101 tacaggaaat ggaattggcg gtcgttgcac tcatcttgct ctgctgatgg caaaaaaaat
     2161 cgctggcaaa aaacaaataa ctttttatgc tcttgccagc gatggaaatg ataatttgga
     2221 taatgtttca ggtgcctggg ttgatcaaaa cacagctaat aaattgcaga ataaagggat
     2281 agacatctcg tgtgttatta aaaattgtga ttcctacaca gccctaaaaa ccgttgaatc
     2341 aataatccct gcttgggttc agcccctcaa tttaaatgat atctacctcc tggaaataaa
     2401 gtaag
//
LOCUS       DAOSTH010000142         2374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_40805_length_2374_cov_10.273825, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000142 DAOSTH010000000
VERSION     DAOSTH010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40805_length_2374_cov_10.273825"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            330..2126
                     /locus_tag="PLB65_08350"
     CDS             330..2126
                     /locus_tag="PLB65_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOQ78577.1"
                     /translation="MTIQRYSVNQYPIQTIISWIQSNEIAIPEIQRPFKWDATKVRDF
                     IDSLYYGYPVGYFIIWRNPNVRLKDGTISIGKRILIDGQQRVTAIMASLLSTEIVNSE
                     YIKKKITIAFNPVRKEFAVSDAAIKKDKKWISDISDVFHPDFSLLDFIERYCDQNEEL
                     DKNAVFKSVELLKGIVNNSIGLIELNSDLDIDTVTEIFIRINSQGVVLSQADFVMSKI
                     AANESYSGNLLRKAIDYFCHLAVEPSFYRQLKEIDKEFAQTEYFQKMSWLKDEIDDLY
                     DPSYTDVLRVAFTYKFKRGRLQDLVALLSGRNFETKQYEETIAEESFYQLSEGIKAYI
                     NENNFKKFVMIIRSAGFTTSSLIRSQNALNFSYILYLSLRESGVSQAEIESLVRKWFV
                     LSVLTSRYSGSPESVFDYDIKHINNDGAKKYIDDIIAAQLPENYWEIALPLQMDTSVT
                     SSPLFNVFLAAQIKNNDKGFLSRDVAVKDLVELKGDSHHIFPKEYLKKQGFNRSIYNQ
                     IANYAMAQSEINIAIGASAPSIYLNDVLEQCRGGQLKYGGIQSEEDLMKNMQIHCIPL
                     EIFQMTASDYNDFLEIRRKLMSRKIRDYFLSL"
BASE COUNT          785 a          380 c          467 g          742 t
ORIGIN      
        1 gattcattgc aattctatgc atttggagaa cttgatgcac ggaagatatc atcgacaaat
       61 agcattgagc gactgaatgc tgagattcgg aggcgctaaa aggtgttggg gatatttcca
      121 agcgtcgatt catatctgcg actcttatgt tcgtacctca tggaatacga agaggattgg
      181 cagacagggc ggtgttatat ttcagaggct tcgcttgcaa agcagcaaac tttgctatat
      241 caagtaacat aagggaaggg aagctcctat gactgctttg aaaccaaatt gcgaacataa
      301 cttgacacga tcgtatggag gcctattaaa tgacaattca aaggtattct gtaaatcaat
      361 atcctattca gacaatcatc agctggattc aatctaatga aattgctatt cctgagattc
      421 agcgaccctt caaatgggat gcaacaaagg ttagagattt tattgattca ttgtattatg
      481 gctatcctgt aggatatttt atcatttggc gcaatccaaa tgttcgcctt aaggatggca
      541 caatctctat tggcaaacgt atcctgatag atggccagca aagagttact gcaattatgg
      601 catcactttt atctactgag attgttaata gcgaatatat caagaaaaaa atcactattg
      661 cttttaatcc agtaagaaag gaatttgccg tttctgatgc tgcaataaag aaagataaga
      721 agtggatctc ggatatatct gatgtattcc atcctgactt ttctcttctt gactttattg
      781 aaagatattg tgatcagaat gaagagcttg acaaaaatgc tgttttcaag agcgttgaat
      841 tattaaaagg tattgtaaat aatagtattg gcctcattga actcaattcc gatttggata
      901 tagatactgt cacagagata tttattcgaa taaattccca aggcgttgtt ttaagccaag
      961 ctgattttgt tatgtctaag atagcagcaa atgagagcta tagcgggaat ttattgcgaa
     1021 aggctattga ctacttttgt catctagcag ttgaaccatc attttacagg cagctcaaag
     1081 aaatagataa agaatttgct caaacagaat attttcaaaa gatgtcttgg ttaaaagatg
     1141 aaattgatga tttgtatgat ccgtcatata cagatgttct tagagttgca tttacttata
     1201 aattcaagcg gggcagactt caagatcttg ttgccctttt gtccggaaga aatttcgaga
     1261 caaaacaata tgaagaaact atagcagaag aatcctttta tcaattatca gaaggaatta
     1321 aagcctatat aaatgagaat aactttaaga aatttgtcat gattatacgc tccgctggat
     1381 tcactacctc ttccctcatt cgatctcaga atgcactaaa cttttcatat atcttgtatc
     1441 tttctcttag agaatctgga gtatcacagg cagaaattga atctcttgta cgcaaatggt
     1501 ttgttttatc agtacttacc agccgctatt caggctcgcc agaatcggta tttgattacg
     1561 atattaagca tataaataat gatggtgcta aaaaatatat tgatgacata attgctgcgc
     1621 aacttcctga aaattattgg gaaatagccc ttcctctaca aatggatacc tctgttacga
     1681 gtagcccttt gtttaatgtt tttcttgccg ctcagattaa gaataatgat aaaggatttc
     1741 tttcaagaga tgttgcagtc aaagatctcg tcgaattgaa aggtgattcc catcatattt
     1801 ttcctaaaga gtatttaaaa aaacaaggat tcaatcgttc aatatataat caaatagcta
     1861 actacgctat ggcccaaagt gaaataaata ttgctatagg cgcttctgcc ccttctatat
     1921 atcttaatga tgttttagaa cagtgtaggg gaggacagtt gaaatatgga ggcatacaat
     1981 cagaggaaga tctaatgaaa aatatgcaga ttcattgtat cccattggag atatttcaga
     2041 tgaccgctag cgactacaat gattttttag aaattaggcg taaattgatg tccagaaaaa
     2101 taagggatta tttcttatct ttatgagctt cttttaaaag tccaagaata ttctgacatg
     2161 aattgtagga ataagaaata gagacaggat tttgcaggat aaaccatgaa gtcaggatta
     2221 acaggtacaa aaaagtaaga aaaagggcat taagtgagct ttttgcgagg taaaagctta
     2281 tagctctatt cgatgggtca tgagagttat ggcttaagat attcgtcttt agatgaggct
     2341 aggtagaata aactgctcaa gaatcttgat tgcg
//
LOCUS       DAOSTH010000143         2352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_41352_length_2352_cov_16.052242, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000143 DAOSTH010000000
VERSION     DAOSTH010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41352_length_2352_cov_16.052242"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1252)
                     /locus_tag="PLB65_08355"
     CDS             complement(<1..1252)
                     /locus_tag="PLB65_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOQ78578.1"
                     /translation="MLAKPEKCQKITIGATMQAIQLKNIHFTYPDQYQEILSGITLDI
                     CDGQKIALIGKNGCGKSTLLKIIMRELQPTSGSISYPNSIPKIGYLPQDLMVRGSITV
                     RDYLLQVDINSYNIINEINALSRIDNLQEKDGLRLAELWQEYDQSNVIMWEQTVDDVI
                     REMDLNLLAQQSFNTLSAGETTRVQLASLLLQNPDILILDEPTNHLDLKELVWLENWL
                     NAYPKAILYVSHDRIFIDNTASKIIELEKGKVLIRTGNYQSFTKDKQELQEHQLKQYT
                     ERKKLIGKLQEAAQKRHNWANSFQKETRPEGGGFKYESIFNAARTMNQQAKHIEERII
                     MLQTRFPIEKPIKDKTRKIVFSPATGQQKELLSLKDIDFAYGEQQVLHNLYFYIWSGE
                     KIWLAGANGKGKTTLLQIIAGDLKP"
     gene            1732..2226
                     /locus_tag="PLB65_08360"
     CDS             1732..2226
                     /locus_tag="PLB65_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015425517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HOQ78579.1"
                     /translation="MIELRFGDITNFKGEAIVNAANSTLLGGGGVDGAIHRAAGKGLL
                     EECKTLGGCKTGEAKITRGYNLKAQFVIHTVGPIWQGGNSGEAELLASCYKKSLELAF
                     SHRIKSIAFPNISTGVYRFPKDEAAKVAISTAQEFVEEHPEMEIYFYCFDLENYNIYQ
                     KLIG"
BASE COUNT          671 a          408 c          450 g          823 t
ORIGIN      
        1 taggtttcag gtcgccagca attatttgca gcagagtagt tttacccttt ccgtttgctc
       61 ctgcaagcca gattttttca ccgctccaga tataaaaata gaggttatgc aaaacctgtt
      121 gttcgccgta agcaaaatca atatctttaa gagaaagcag ttctttttgt tgtcctgtgg
      181 caggagaaaa cactattttt ctggtcttat ctttgatcgg tttttcaatt ggaaagcgag
      241 tttgcagcat aattatgcgc tcttcaatat gttttgcctg ttgattcatt gttcgggcag
      301 cattaaaaat tgattcatat ttaaaaccac ccccctcagg acgagtttct ttttgaaagc
      361 tatttgccca gttatgtctt ttttgggctg cttcttgcag ttttccaatc agttttttgc
      421 gctcggtata ttgttttaac tggtgttctt gcaattcctg tttatcttta gtaaaacttt
      481 gataattgcc tgttctaatc aggacttttc ccttttccag ttcaatgatt tttgaagcag
      541 tgttatcaat aaaaattcta tcgtgggaaa cataaaggat agctttagga taggcattta
      601 gccaattttc caaccagacg agttctttta ggtcaagatg atttgttggt tcatccaaaa
      661 ttaatatatc cggattttgt aataaaagcg aagcaagctg aacgcgggtt gtttctcctg
      721 cggaaagagt gttgaaactt tgttgggcaa ggagattcaa atccatttct cttatcacat
      781 catccaccgt ttgttcccac attattacat tactttgatc atattcttgc cacaattcag
      841 caagacgcaa gccatctttt tcctgaaggt tgtctattct ggaaagggca ttgatttcat
      901 taattatatt ataactgtta atatctactt gcaatagata atcacgcacg gttatactac
      961 cacgaaccat aagatcctga ggcagataac caattttggg aattgaatta gggtaagaaa
     1021 tagaaccgga agttggctgc aattctcgca ttattatttt tagcaaggtt gattttccgc
     1081 acccattttt tccgataagg gctatttttt gtccgtcaca aatatccaga gtaattccgg
     1141 atagaatttc ttgatattga tcgggatagg taaagtgtat atttttcagc tgtattgcct
     1201 gcattgtagc tcctatagtt attttttggc atttttctgg tttggcaagc ataacagctt
     1261 tgtagcagaa ctaaaaatat agattctttt ttcctatgct gttatgcatt cattttagag
     1321 ttgatcacag attttacctc ctattcttaa atttgtaatc ataatttgca gttatgagga
     1381 aaaggtcaag gaaaataatg cttttcccgt tccttgcttc actctgtaga ctttcggtaa
     1441 cgattctcta atactctaat aacgaaacta ctttagcgtt acttcattgt tactttatta
     1501 ttacggagtt gccttagaac cgattaagca ataaggagtt atgctatttt ttcttttagg
     1561 gtcttgtgtt tgtgcaaaaa atttgctaga acatctttta tttatgtttg ctgttgccaa
     1621 tggcttattt tcattgacgg aattctgcat aatcattaag tgttttggaa tggattaaca
     1681 tataagttga aaaagaactt accagcccaa ttatcaaatg gaggtgaaag aatgatagaa
     1741 ctcagatttg gtgatataac taattttaag ggcgaagcaa ttgtaaatgc agccaatagt
     1801 acccttttag gtggtggagg agtagatggt gcaattcata gagctgctgg aaaaggtctt
     1861 ttagaagaat gtaaaacatt gggcggttgc aaaacaggtg aggcaaagat taccaggggt
     1921 tataatctta aggcacaatt tgttatccat acagttggtc ctatttggca aggaggcaac
     1981 tcaggggaag cggaactttt ggcttcttgt tataaaaagt ccctcgaact ggcattttca
     2041 catagaatca aatccattgc ctttcccaat atatctacag gtgtatatcg ttttcccaaa
     2101 gatgaagcag ccaaagtagc tatttctaca gcacaggaat ttgtagaaga gcatcctgag
     2161 atggagatct atttttattg ttttgatctg gaaaattaca atatatacca aaagcttata
     2221 ggatagtatg caaggggatg ggaaacatat aacatatctt ttacgagtgc tttacgcacc
     2281 catcgctatc attgtgtcgc cctgaggggc ttttattggt tatataatac aatcctctaa
     2341 atcctaaaat cc
//
LOCUS       DAOSTH010000144         2194 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_45464_length_2194_cov_11.092099, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000144 DAOSTH010000000
VERSION     DAOSTH010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45464_length_2194_cov_11.092099"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(454..1659)
                     /locus_tag="PLB65_08365"
     CDS             complement(454..1659)
                     /locus_tag="PLB65_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78580.1"
                     /translation="MNEWGYRQLRTNKLVRLCTEYLLKDTITAKQVYGLCRLTWITNS
                     NDSANGRYIKSTKLPALSNIFEVDFLDYPYDEIGKKIAELTGNSVKVVHDLIYEGTGY
                     TNFYNAYRVSAKNWISENIDEIIPLIHIGFNIRNDNEAESIASAISLLPGIPKGNKAP
                     GKMHPENLLSPLFFSLDKRRRFPLINGNEGVTKILKQLRVHNQDFVIKFKAIVGLLGK
                     GDIKTAIDVDCLGGELPYFVSIDDEVPERKRLTKKTDKELELKDEDDVIVIKKALSTK
                     AKRLHNKLTNILLDRLSQFELHEGNSNENKFDVLVKNITSNKEDILIEVKSASDISNV
                     RMAVGQLMDYSRQLDNHNQTYEAVLMPEKPDKHVIDFLDFYDFGIIWIEENEIYANNK
                     AFPFKYKKI"
BASE COUNT          583 a          487 c          324 g          800 t
ORIGIN      
        1 cacaaaatta aacttttatg cttaactctg tgtcccaact aagttagtaa ttccaatgtt
       61 ttcattttct ttctctttaa gttaattaat gattgttata tttgatttct gaaaacaaat
      121 tctaaacatc tatcttaaaa aggatccttt caaaaagcag tgttttaacc cgagtcgagc
      181 caaggcatca gtcattctcc ttttgctccg ccagctccgc ttcattccgg accacaaact
      241 cttgacgctc caggcttaat cggtattcgc tccgctcatg atcctttaac cccacccagc
      301 gaacttcgtt cgcctcccgc cctggaacca gcggcgttcg ctgcgctcac tcgccgtcac
      361 aaacgctttt gtggcacaga atatttgata taatcatttt attaccaatg ttttataaac
      421 tacgttgtgc aaagtttatt ttttttacat caactatatc tttttatatt taaatgggaa
      481 tgccttatta ttcgcatata tttcgttttc ttcaatccat atgataccaa agtcataaaa
      541 atctaagaaa tcaatgacat gtttatctgg tttttcaggc atgagtactg cttcataagt
      601 ttggttatgg ttatctaatt ggcgcgaata atccattaat tgtccaacgg ccattcttac
      661 attggatatg tctgatgctg atttcacttc aatcaaaatg tcttccttat ttgaagttat
      721 gtttttaacc aaaacatcaa atttattctc atttgaattt ccttcatgta attcaaattg
      781 cgacaatcta tctaataaaa tatttgtcaa tttattgtgc aatcttttgg ccttggttga
      841 taatgctttc tttattacta tcacatcgtc ttcatctttc aattctaatt ctttatctgt
      901 cttttttgtt agtcttttcc tttctggtac ttcatcatca atacttacaa agtatggaag
      961 ttctccacct aaacaatcaa catcaatagc ggttttaata tcacctttac ccaatagccc
     1021 tactattgct ttaaatttaa tgacaaaatc ttgattgtga actctcaatt gcttaagaat
     1081 cttggttacg ccttcattac cattaattag ggggaatcta cgtcttttat caagagaaaa
     1141 gaaaagtgga gaaagtaaat tctctgggtg catttttcca ggtgctttgt tcccttttgg
     1201 tattcctggc agaagtgaaa tagctgaagc aatggattct gcttcattat cgtttcgtat
     1261 attaaaacca atgtgtatta atgggataat ttcatctatg ttttcagata tccaattctt
     1321 tgcgcttact ctataagcat tataaaagtt tgtatatcca gttccttcat aaattagatc
     1381 gtgaactact tttacgctat tacctgtcag ttcagctatc tttttcccaa tttcatcata
     1441 tggataatct agaaaatcta cttcaaaaat gtttgataag gctggtaatt ttgtactttt
     1501 aatatacctg ccatttgctg aatcattact atttgtaatc catgttaacc tacataatcc
     1561 ataaacctgc tttgcagtaa tggtatcttt aagcaaatat tcagtacaaa gtcgtaccaa
     1621 tttatttgtc cttaactgtc tatatcccca ttcgttcatt ttaatataag tttaagtaaa
     1681 ttataagtct atgtcaatca atatggtgtt gtgttttaga gttttgcaca acttgtttat
     1741 atatatttat gtgtatctat accctcaaat tccggggtca taaataacca tccaaccaaa
     1801 cccctcaagg cctccttttc ctgaattccc acggcgcgac cgccctcgga tcactccata
     1861 aacccctttt attatcccgt gctgtctgct ccgcttgcgc atacgcttcc gacttgtcgt
     1921 aatgcttgta atgccaggcc agcccgtcct gaatcatctc cttcccgaca ttcctcccgt
     1981 cctctgttgc aaccctggcg atataccggc cccatctatc ttggtcgttt acgtcgacgg
     2041 ttacgttttg ctcaaaatca acgagctgag gtgttccctg gctttcgttc caaaggcttg
     2101 cttcttctcc ggcgcatcga tcccgtgtat cctgaatttc agttggaggt tgtcgctgtt
     2161 caatcccgta aaggtatccc catcagaaat cccg
//
LOCUS       DAOSTH010000145         2123 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_47575_length_2123_cov_12.393617, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000145 DAOSTH010000000
VERSION     DAOSTH010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47575_length_2123_cov_12.393617"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="PLB65_08370"
     CDS             complement(<1..349)
                     /locus_tag="PLB65_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HOQ78581.1"
                     /translation="MIYEKELSDRIIGCAIAVHKELGAGYLEKVYERALCVEFKYQGI
                     SYVSQVPIQVFYRDQIVGDYVADIIVESKIILELKACNIIHAMHSAQIINYLCATGLK
                     VGYILNFGNEAKLE"
     gene            complement(384..857)
                     /locus_tag="PLB65_08375"
     CDS             complement(384..857)
                     /locus_tag="PLB65_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOQ78582.1"
                     /translation="MIQEKRMANGVRWLLWGWLILVLVLNVVPLGNETNRGLSGNKIF
                     QFRLDYVVHSLTFLVFAWIWVLGKIKDVCWFESYEVLKFGGIIFVSAMGIELLQIFVP
                     YRTFNPMDMMANLFGAILTMLFIFISHRVHRKHRKMINNTKIQATECTQDTEKVI"
     gene            complement(982..2097)
                     /gene="wecB"
                     /locus_tag="PLB65_08380"
     CDS             complement(982..2097)
                     /gene="wecB"
                     /locus_tag="PLB65_08380"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HOQ78583.1"
                     /translation="MLKVITVVGTRPEIIRLSCVIPLLDKYTNQILVHTGQNYDYELN
                     QIFFEDLELRQPDYYLNVNTSSLGYVLGETLIKIEEILDKEKPDAMVILGDTNASISA
                     IMAKRKHIPIYHLEAGNRSFDNNVPEEINRKMIDHIADFNLVYTENSRRHLLSEGLLH
                     RRIYLTGSPMFEVLQHYLPKIKRSNILETLKLEPQKYFLISVHREENVDYPDNLKKIS
                     IFLNTLAEQYHYPVIVSTHPRTRKRLENLNSFKFNPLIQFMKPFSFTDYNFLQMNSTC
                     VISDSGTISEESAILKFPAITIRNSMERPEAIDAGTIILSGFDPDIVLDSIKVAIEEG
                     AKYTKICPEYEIDNCSMRVLKLILGTAKLHKQWSGLY"
BASE COUNT          669 a          432 c          303 g          719 t
ORIGIN      
        1 attctaattt tgcctcattg ccaaagttta agatatatcc aacttttagc cctgtagcac
       61 atagataatt tattatttgt gctgaatgca tggcatgaat tatattacaa gccttaagct
      121 ccagtataat tttactttct acaataatat cagcaacata atctccaaca atctgatctc
      181 tataaaaaac ttgtatagga acttgagaaa cataactaat accttgatat ttaaattcta
      241 cacataaagc tcgttcatat actttttcca aatatccggc accaagctct ttgtgaacag
      301 caattgcaca accaataatt ctatcactca gctccttctc atatatcata aatattggct
      361 tttcctcttt cttattttga aaattatata accttttcgg tgtcttgggt gcactcggtg
      421 gcttgaattt ttgtattgtt aatcattttt cggtgttttc ggtgcactcg gtggctaata
      481 aaaataaaca gcattgtcaa tattgctcca aagagattcg ccatcatatc catcggatta
      541 aatgttctat agggcacaaa aatctgtagc aattcaatcc ccatagcaga aacaaatata
      601 atccctccaa acttcaaaac ctcataactc tcaaaccaac aaacatcctt tatcttccct
      661 aaaacccata tccaggcaaa aaccagaaat gttaacgaat gcactacata atccagccga
      721 aactggaaga ttttattacc actcaaacct ctatttgttt cattccccaa aggcacaaca
      781 ttcaaaacca aaaccaaaat caaccatccc cataataacc atcttactcc attagccatc
      841 cttttttctt gtatcataat cctatttata ttctcagtat tctcagtgct cttagtgtcc
      901 tcagtggtta caatttttcg gtgctttcgg tgttctcggt ggcttatttc tcagtgccct
      961 cagtgtcccc agtggtcgca attaatataa cccactccac tgcttatgca atttagcagt
     1021 ccccaaaatc aatttcaaca cacgcattga gcaattatca atctcatatt ccgggcatat
     1081 ttttgtatat ttagcacctt cttctattgc aactttaatt gaatcaagca ctatatcagg
     1141 atcaaaaccg gaaaggataa tcgtcccggc atctattgct tccggacgtt ccattgaatt
     1201 cctgattgtt attgcaggaa atttaaggat tgcactttct tcacttatag ttccactatc
     1261 tgaaataacg caggtagagt tcatttgcaa aaagttatag tcagtaaaac tgaatggctt
     1321 cataaattgg attaagggat taaatttgaa actatttaaa ttttctaatc gtttacgggt
     1381 tctgggatgc gtgctaacaa tcacaggata atgatattgc tctgcaagag tatttaggaa
     1441 aatgcttatt ttcttcaaat tatcaggata atctacattt tcctctctat gtacagagat
     1501 gagaaaatac ttttggggtt ccagttttaa ggtttctaaa atatttgatc gtttaatttt
     1561 aggtaaataa tgttgtaaga cctcaaacat aggagagcca gttaaataaa ttcttctatg
     1621 aagaagccct tcagataaaa gatgtcttct tgaattctcg gtatagacca aattaaaatc
     1681 cgctatatga tcaatcattt tccggttgat ttcttccggc acattgttat caaaacttct
     1741 gttacctgct tctaaatgat agataggaat atgttttcgt ttagccataa tagcagatat
     1801 agaagcgtta gtgtctccaa gaataaccat tgcatccggt ttttccttat ccagaatttc
     1861 ttcaatttta atcagcgttt cacctaaaac atatcctaag ctggaggtat ttacattaag
     1921 ataataatca ggttgccgaa gctctaaatc ctcaaagaaa atctgattca gttcatagtc
     1981 ataattttga ccagtgtgaa ctaagatttg atttgtgtat ttatccaata aaggaattac
     2041 acaggataat ctgattattt caggccttgt gccaacaact gtaattactt taagcatttt
     2101 ctatttcccg cagatttcgc aga
//
LOCUS       DAOSTH010000146         2092 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_48584_length_2092_cov_11.194894, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000146 DAOSTH010000000
VERSION     DAOSTH010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48584_length_2092_cov_11.194894"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..237
                     /locus_tag="PLB65_08385"
     CDS             58..237
                     /locus_tag="PLB65_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78584.1"
                     /translation="MIKNRLEKIEQKLDEGKPRVAAIFRIKYHDGTVVEHKQERKGYK
                     GNPVVIECDEDDWRL"
     gene            279..1385
                     /locus_tag="PLB65_08390"
     CDS             279..1385
                     /locus_tag="PLB65_08390"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOQ78585.1"
                     /translation="MSMKIKTLILLVILFQASILFGGESIQTIDQEFREEVLKGIRQY
                     RFYKYTEYSSGFYTVSSEIELDDNTSIRMVSFIDSGNLRGKLEFNYSYHQSINNLYFD
                     SVLTFIADEEEIRLFDDTPDIYGNLFSDSFFSFHFWLTELRRGDLRKISESKNLKLKI
                     DETTLNLSLGQIKSIQQYIKFILLTNLTDADIEYLSSIVTIEYVDFNDPKYRLPLPTT
                     DKIIAPAPEPEPFSLPTLEPALETDLKPLTISLLFDAIENNQIDYIKQAMVSKIDLKG
                     INDEGFTPLLWAALKSSNPEVISLLIGNGADINKTDTLGRTPLIISATYNENVEILKR
                     LIESGADPNSTSIDKDSDTALYGATIFNPNLNII"
     gene            1538..>2092
                     /locus_tag="PLB65_08395"
     CDS             1538..>2092
                     /locus_tag="PLB65_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78586.1"
                     /translation="MTKILQDYKFWGNVKYSRWYKYNLLDANAGYEKRWESDSFRDSI
                     ILNIRNACAVQLVNIEKYEPFKEIVFNKKQEIINYLRCGSLYSWDFLFAEKPSIHVDN
                     TYYLNQKIFDQGEFTYSDYFFMFPNIKLTKFGYDNFEAIVDELSNIIEVRHKQPENLI
                     LKYYYKFRRDNSRYCRNFSKLLLNN"
BASE COUNT          746 a          323 c          385 g          638 t
ORIGIN      
        1 cacaatggat tacacaggat atgcttccaa cctctccgat aggacaagga agtaaagatg
       61 ataaagaaca ggctggaaaa gattgaacag aaactggatg agggcaagcc tagagtggca
      121 gccatattca gaattaaata tcatgatgga actgtagtgg aacataagca ggagcggaaa
      181 ggatacaagg gaaaccctgt agtcattgaa tgcgatgagg atgattggag gctgtgattc
      241 ttattaaggt tgttttttga attatatcga cttatgctat gagcatgaaa atcaaaacac
      301 tgatattgct agttatccta tttcaagctt caattctttt tggtggtgaa tcaatacaga
      361 caatagatca agaatttaga gaagaggtgt tgaaggggat aaggcagtac aggttttata
      421 aatataccga atatagctct ggcttttata cagtttcatc agaaatagaa ttggatgata
      481 atacgagtat ccgaatggta agttttatag attctgggaa tctaagagga aaattagaat
      541 tcaattatag ctaccaccaa tcaatcaata atttgtactt cgatagtgtc ttgacattta
      601 ttgctgacga agaagaaatc cgcttatttg atgatacccc agatatttat ggcaatctat
      661 ttagtgacag ctttttttct tttcatttct ggttaactga attgagaaga ggtgatttga
      721 gaaagatttc agaaagcaaa aatctgaaac ttaagattga tgagactaca ctaaatctat
      781 ctttggggca gattaagagc attcaacaat atatcaagtt tatccttctt acaaacctca
      841 cagatgcaga tattgagtat ctgtcttcta ttgttacaat agaatatgta gattttaatg
      901 atccaaaata taggttacct ttaccaacga ctgataaaat tatagcacct gctccagaac
      961 cggagccttt ttcattacca acactagaac cagccctaga aactgatctc aaacctctca
     1021 caatttcttt gctatttgac gcgatagaga acaatcaaat tgattatatc aaacaagcaa
     1081 tggtttcaaa aattgaccta aaaggaataa atgatgaagg tttcacccct ttattgtggg
     1141 ctgctcttaa atcaagcaat cctgaagtta tctctctgct tatagggaat ggagcagata
     1201 ttaataaaac agatacactt gggcggacac cacttataat atccgccact tataatgaga
     1261 atgttgaaat tctaaaaagg ttgatcgaat caggcgcaga tccaaattca acaagtattg
     1321 acaaagatag cgacacagca ctttatggag ctacaatatt taacccaaat cttaacatta
     1381 tttaatggat tgaaaagtga tgaattactc cgaattcata ttggaatacc aaccgtttac
     1441 aaataccgac aatttcatcg atgttgagta caatcatatc attaagagaa taagcacttg
     1501 cgaaacccgt aatgaagtaa tgttctttaa aagaactatg acaaagatac tccaagacta
     1561 taaattttgg ggaaatgtaa agtattccag atggtacaag tataatcttt tagatgctaa
     1621 tgccggttac gaaaagagat gggaaagtga tagttttagg gattcaataa ttctgaatat
     1681 tagaaatgcg tgtgctgttc aattggtaaa tattgaaaaa tatgagccat ttaaggaaat
     1741 tgtattcaat aagaaacaag aaatcatcaa ctatttgagg tgcgggagtt tgtactcttg
     1801 ggattttctt tttgctgaaa agccaagtat tcatgttgat aacacttact atttaaacca
     1861 aaaaatattt gatcagggtg aatttactta ttctgattat ttttttatgt ttcccaacat
     1921 caagctaact aaatttggat acgataattt tgaggcaata gttgatgaac tttctaacat
     1981 aatcgaagtg aggcataaac aaccagagaa tctaattctg aagtactact ataaattccg
     2041 cagagataat tctagatatt gtcgcaattt tagtaagctc cttcttaata at
//
LOCUS       DAOSTH010000147         2082 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_48914_length_2082_cov_11.713863, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000147 DAOSTH010000000
VERSION     DAOSTH010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48914_length_2082_cov_11.713863"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="PLB65_08400"
     CDS             <1..1202
                     /locus_tag="PLB65_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HOQ78587.1"
                     /translation="FYQTSVHCAQFQDNNLDGYADRRFTHTSEEVRDYIIGQGKTVNR
                     VYYTSSSAFPKNYNKYHYSDGQAIPSELRKDIAPFYPWTGNNTNIASEINAGKFYVLH
                     RDHGSYTGWKHPNFSVTDISNLTNGDKLPVVFSINCQTGGFLQTECFAEKFIRQSGGG
                     AVGVFAASQISYSGYNDALTVGMFDAIWSNPGLLPNFGSGGISNPNVNTHSDIYKMGH
                     VLNQGLLRMGQTWGLDQYTNRIFHYFGDPSMEMYTASPSTFTGVTVTENGTSATVNTG
                     VSNCKITVCSILDMGSSCYEVADNVSSYTFTDIVKPYYISVTKHNYKPYIYPQDIYIQ
                     NYTFTSDRLIIGRNIFVGNNVTPSQTQGPVIIKNGANVIFSAEQDVLLDRGFEVELGG
                     TFEIKKR"
     gene            1731..>2082
                     /locus_tag="PLB65_08405"
     CDS             1731..>2082
                     /locus_tag="PLB65_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78588.1"
                     /translation="MNKKQIADSRSAVIKNLRMKPTFYLILILFFCNLIVAQESFPNL
                     DDKPSWKVVNTITSNPETKTLFEYQFEKDTLIKSVTYQKITNNAGYVRLLNDKVYYRL
                     ESNSKDFLLYDFGLN"
BASE COUNT          692 a          349 c          419 g          622 t
ORIGIN      
        1 cattttatca aacctctgtt cattgtgctc aatttcaaga taataatctc gacggatacg
       61 cagataggcg gtttactcat acaagtgaag aagttcgaga ttatattata gggcaaggaa
      121 aaacggttaa tagggtatat tatacgagta gttctgcatt tcccaaaaat tacaataaat
      181 atcactattc cgatggtcaa gcaatccctt ctgaattaag aaaagatatt gctcctttct
      241 acccatggac tggtaataat acaaatattg caagtgaaat aaatgcagga aagttttatg
      301 ttttacatag agatcatggc tcctatacag gctggaagca tccaaatttc tctgtaacag
      361 acatttcaaa tctcactaat ggggataaac ttccagttgt ttttagtata aattgccaaa
      421 caggtggctt tctacaaacg gaatgttttg ctgaaaagtt tatacgccaa tctggcggag
      481 gtgctgttgg ggtttttgct gcatctcaaa ttagttatag cgggtataat gacgcactta
      541 ctgttggaat gtttgatgct atttggtcta atcccggttt acttccaaat tttggtagtg
      601 gcggaatatc aaaccctaac gtaaacactc attctgacat ttataaaatg ggacatgttc
      661 ttaaccaagg attattgcgt atgggacaaa catgggggct agatcaatat acaaatagaa
      721 ttttccatta ttttggtgac ccaagtatgg aaatgtatac cgcaagccct tcaactttta
      781 caggtgtaac agttactgaa aatggtacat cagcaactgt taatactggc gtatcaaatt
      841 gtaaaataac cgtttgtagt attcttgaca tgggtagcag ttgttatgag gtggcagata
      901 atgtatcgtc atacactttt acagatattg ttaaaccata ctatatatct gtaacaaagc
      961 ataattataa accatatatt tatccacaag atatttatat tcaaaattat acatttacct
     1021 cagacagatt aataattgga aggaacatat ttgtaggaaa taatgttacc ccgtctcaaa
     1081 cacaaggtcc tgttatcata aaaaatgggg ctaatgttat ttttagtgca gaacaagatg
     1141 tacttttaga cagaggattt gaagtagaat taggaggaac atttgagata aaaaagagat
     1201 aatatgatga tgtacaggtg ctaacaaacg gacataaaaa cattggaggc aaagagatta
     1261 ttcgagcctc ggtactcgca tcaaagttaa atgtgcctta attgtgaagt agctccgaaa
     1321 tccctaacgt ttcaggggat gcggcattga cgttcaccag aaagtggtgg tggccaccat
     1381 aagtggcgat ggcctaaaga aggaaacccg tgagtttggt accttcactc gatctttgac
     1441 agaactgaaa gactggttat tggaaaaggg cgttacccac gtagcgatgg agagtaccgg
     1501 tatttattgg aaaccggtgt accaggttct tgagcccaca tcgctcaagg tttgggtagt
     1561 taatgctcgt cacatcaagt acgttccggg tcacaagacg gataaacagg acagtgcctg
     1621 gatttgcaag ttgttgtaaa acaaaacccc gaaccaataa ccccgcattc atcgaccagt
     1681 gagtgctctt ttaggttttg tccaaacgga atcgtaggga atataatata ttgaataaga
     1741 aacagatagc agatagcaga agtgctgtca ttaaaaattt acgtatgaaa ccaacattct
     1801 acttgatttt aattcttttc ttctgcaacc tgattgttgc acaggaaagc tttcctaatt
     1861 tggatgataa gcctagctgg aaagttgtga atacaatcac gagtaatcct gagacaaaaa
     1921 cattatttga atatcaattt gaaaaggata ctctgattaa atctgttact tatcaaaaaa
     1981 tcacaaataa tgccggatat gtcagattgt taaatgataa agtctattac agactggaaa
     2041 gcaacagtaa ggatttttta ttgtatgatt tcggtctgaa tg
//
LOCUS       DAOSTH010000148         2073 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_49217_length_2073_cov_11.469772, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000148 DAOSTH010000000
VERSION     DAOSTH010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49217_length_2073_cov_11.469772"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            253..1836
                     /locus_tag="PLB65_08410"
     CDS             253..1836
                     /locus_tag="PLB65_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78589.1"
                     /translation="MKKSMKKKSSFPFFQFLLAAFPVVSLLGNNIQEVSLRAGLRPLF
                     YILLVTVCVWAALSFATKSFQKASFITLWVLLLFFTYGRVYSAFSTTNSFGQILGHHR
                     FLLPLWFVLGAGGVYVILKFAKENNNLTAFLNLFSVFLFIIPVYQVINYEIRVNEAEK
                     HLSQIEVASTQEDVTLHPETLPDVYIIILDAYLRSDVLKTTFNLDNGAFIETLTKMGF
                     YVPSCSMSNYAYTPLSMASFLNMEYVQNLSSDFVPPNTDRTLLFELINHNTVRKNLES
                     LGYSTYSLASYQNIAWTDADHYYPLDAASLPALDDQTVLSGFERMFYDTTIVRILFDY
                     NGGKTTSNKIPVNYPFADHVRQQWTILKELQQISSLRGPKLVWAHLTMPHAPYVFYPD
                     GSLIPDPPPLPWVVPLPWDQYLQYYSWGVQYTNNAIVPVLEAIISNSDTPPIIILTGD
                     HGADSDNRLAVLSAFYVPDQVKEKIPEDITLVNYFRVIFNEMYGSNYQILDNFSYLST
                     QEEPYNFTLYPETMPNCIP"
BASE COUNT          591 a          425 c          397 g          660 t
ORIGIN      
        1 caccgctatc atcatgaagg gcaggctgag cagtttgtca tccgcattta cagccagaaa
       61 agttagtcca atataagcat agaaagaggc gttccttggt agaattaatt agctgacttg
      121 gtaaggtttc aaacaagatc aaataaggca ataaaactag aattagattt gtactccatt
      181 aattttctca tttggtaagg actataccga ttctaaagtt atccgtaggt tgaaagtggt
      241 aagatcatag aaatgaaaaa atccatgaaa aagaaatcat ctttcccatt tttccagttc
      301 ttattagctg cttttcctgt agtatcgctt ctaggaaaca atattcaaga agtatcactg
      361 cgtgccggac ttcgcccgct gttttatatc ctccttgtta cggtttgtgt gtgggctgca
      421 ctttcctttg ctaccaagtc gtttcaaaaa gctagcttta ttactctctg ggtactgcta
      481 ttattcttta cgtatggaag ggtttacagc gcattcagca ctactaactc ctttggtcag
      541 attttaggac accatcgatt tttgttgcca ctttggtttg ttttaggtgc tggtggcgta
      601 tacgtgattt tgaaatttgc taaagaaaat aataatctca cagcatttct aaatttgttc
      661 tcggtatttt tatttattat tcctgtatat caagttatca attatgaaat acgcgtaaac
      721 gaggctgaaa aacacttgtc acagatcgag gttgcctcga ctcaggaaga tgtaaccttg
      781 cacccggaga cattacccga tgtctatatc atcattttgg atgcctattt acgcagcgac
      841 gtgctaaaaa ctaccttcaa cctggataac ggagcattta ttgagacgtt gactaagatg
      901 ggtttttatg tcccctcatg cagtatgagt aattatgcat atactccgct ttctatggca
      961 tccttcctta atatggaata cgttcagaac ctgagtagtg attttgtgcc cccgaatacg
     1021 gataggactc tcttatttga gctaatcaat cacaacactg tcagaaagaa tctagaatca
     1081 ctgggatatt caacgtattc tctagcttca tatcaaaata ttgcctggac cgatgcagat
     1141 cattattatc cgttggatgc tgccagcctc ccggcattag atgaccaaac ggttttgagc
     1201 ggtttcgaga gaatgttcta tgatacgacc attgtgcgta tactttttga ctataacggc
     1261 ggaaaaacaa catctaataa aatcccggtc aattacccgt ttgctgatca tgtgcgtcaa
     1321 caatggacaa ttctgaagga attacaacaa atttcatctt tgagaggtcc aaagctggta
     1381 tgggcacatc ttaccatgcc acatgcaccc tatgtatttt atccagatgg ttcgcttatc
     1441 ccagatcctc caccgcttcc atgggtagtt cctttaccat gggaccaata ccttcagtat
     1501 tactcttggg gggttcagta tacaaataat gccattgtgc cggtactgga agcaattatc
     1561 agtaattcag atacccctcc tatcattata ttgacaggag atcatggcgc tgatagtgac
     1621 aatcggctgg cagttttaag cgctttttat gtaccagatc aggtcaagga aaaaattccg
     1681 gaagatatta ccttggtaaa ctatttccga gtaattttta atgagatgta tggatctaat
     1741 taccaaatcc tagataattt ctcatatcta agtactcagg aagagcctta taattttaca
     1801 ttatacccag agacaatgcc caactgtatc ccttaatcaa aaatatcttt aatcacttta
     1861 ttttattttg catattctca cccgcctttg caaccagtaa atcctttcct gcggatctat
     1921 ttgtgaggaa gttaaaattt aaatgaacta ttctcgaagc agggtatggg gaacttccct
     1981 cttttgatta tagcggcaat cttaaggtga agatagcccc accttgcggg tgatttgaaa
     2041 cagtgagggt gccgccatgc gcttcagcca gtt
//
LOCUS       DAOSTH010000149         2039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_50413_length_2039_cov_9.025202, whole genome shotgun sequence.
ACCESSION   DAOSTH010000149 DAOSTH010000000
VERSION     DAOSTH010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50413_length_2039_cov_9.025202"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            57..1004
                     /locus_tag="PLB65_08415"
     CDS             57..1004
                     /locus_tag="PLB65_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78590.1"
                     /translation="MNNPLKYTDPDGESIVAAIIVGVIVGAYIGGAAAEGWEYNPGKW
                     AWDGKTWTGIGLGAVIGGSAGVGFYYAAPALANTGFFAHFGASGTVAAYTVTGGVAGG
                     LAGYGAGFSGGMLYSDGNWGYSHQSGIHGAKVGATVGSVIGAISGNIAAYEPPKAPPP
                     VKNPEKPRWDGPYFQGTEQEAKEMLLSSSKMFNVETSYWSTSKGYYFEPIQGNAYGYS
                     YDPISKTMIDYNVSIGYQSNTISSTYRYTYVENLGGNLYLSPNIFQRSLVYNKAHTHP
                     KSSPPSASDLTFSYLFGIPGIVFGWNGAVYNYGGHGYWR"
     gene            1019..1627
                     /locus_tag="PLB65_08420"
     CDS             1019..1627
                     /locus_tag="PLB65_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78591.1"
                     /translation="MINGILILLLMGSLNSMGQNSLTPCVDFSNEINQIKKYNEHLEK
                     VLMNNLKEDYIIRFIAKPTYKPEYAFQIRKIDNSNFEIEAVFFQENLWNVQSINPSVY
                     VRKRNINKELVLKIDALFKVFTNSISNKDALVIGEDGTSFIFKRKTKDGARCGETWSP
                     NETSPLGKLVCICNVLTEYAKGEYVELEKVHENINKLYDSMK"
     gene            complement(1648..1803)
                     /locus_tag="PLB65_08425"
     CDS             complement(1648..1803)
                     /locus_tag="PLB65_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78592.1"
                     /translation="MCKHPVPIWIITYLVTEDNEVYLVYIYDKSQLENITKEQIIELL
                     KNAGLIK"
BASE COUNT          652 a          311 c          453 g          623 t
ORIGIN      
        1 ggttgcctaa tgcgtgtgag acaacctgtt ttgggcttta ctggtaaggg tattgtttga
       61 ataatccact taagtatact gatccggatg gggagtctat cgttgctgca attattgtcg
      121 gcgtcattgt cggtgcttat atcggaggag cagcggcaga aggctgggaa tataacccag
      181 gtaaatgggc ttgggatggt aaaacttgga caggtattgg tttaggggct gtgataggag
      241 gctcagcagg tgttggtttc tattatgctg ccccggctct cgcaaataca ggcttttttg
      301 ctcattttgg tgcaagtggt acagtcgcag cctatactgt gactggcggg gttgcaggtg
      361 gattggcagg atatggagca ggtttttctg ggggaatgct ttatagtgat ggaaattggg
      421 gatattcgca ccaaagcgga attcatggtg ctaaagttgg ggcaactgta ggttctgtta
      481 tcggtgctat ttctggtaat attgccgctt atgaaccgcc aaaggctccc ccgccagtta
      541 aaaatcctga gaaaccaaga tgggatggac catattttca aggaacagaa caggaagcaa
      601 aagagatgtt attgtcttca agtaaaatgt ttaatgtgga aacaagttac tggagtactt
      661 caaaaggtta ttattttgaa cctattcaag gaaacgctta cggttacagt tatgatccta
      721 tttctaaaac gatgatagat tacaatgtca gtataggcta ccaatcaaat acgattagtt
      781 caacatacag atatacttac gtagaaaatc ttggaggaaa tttatatttg agccctaata
      841 tttttcagag gtcattggtt tataataaag cacatacaca tccaaaaagc tctcccccca
      901 gtgcttcgga tttaacattt tcctatctat ttggaatacc aggcattgtt tttggttgga
      961 atggtgcagt atataattat ggagggcatg gatattggag ataaaaaaga gtcatagaat
     1021 gattaacggc atattaatac tattattaat gggttcatta aattcaatgg ggcagaatag
     1081 tttgactcct tgtgttgatt ttagcaatga aataaatcaa attaagaaat ataatgagca
     1141 tttggagaaa gttttaatga acaatctcaa agaagactac attataagat ttattgcaaa
     1201 accaacatat aaacctgagt atgcatttca gatcaggaaa atagataact caaactttga
     1261 aatagaggct gttttttttc aggaaaattt atggaatgtc caaagcatta atccttcagt
     1321 atacgtgcga aaacggaata ttaataagga attggtcttg aagatagatg cattgttcaa
     1381 agtatttacc aactcaatat caaataaaga tgcacttgtc ataggtgaag atggcacttc
     1441 ttttatattt aaacgcaaaa ctaaagatgg ggcaagatgt ggagaaacat ggtctccgaa
     1501 tgagacctct cctttgggga aacttgtatg catttgtaac gttttaacag aatatgctaa
     1561 aggagaatat gttgaattag aaaaggtgca tgaaaatata aataagctct atgatagtat
     1621 gaaatagcct cgggaaaccg gggcttttta cttaataagc cctgcatttt taagcagttc
     1681 aataatctgt tctttggtaa tgttttcaag ctggcttttg tcgtagatgt acacgaggta
     1741 aacctcgtta tcttcggtta caagataagt aataatccaa attggtactg gatgtttgca
     1801 cacccacctt taccatttat gtggtggcta tttttctaaa caattaacat taaaattatg
     1861 aaaatgaata ttgtaatggc cccataattc atcggacgag attatagaca gaaatctgta
     1921 attttatgat gatgaaaaag agaacattca cccgggaaga gaagcttcgg atcattaaag
     1981 aagccagtga acaaggagta aaattcattc ttgagaagta tgctttgtat cctgccacg
//
LOCUS       DAOSTH010000150         2023 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_50978_length_2023_cov_10.387703, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000150 DAOSTH010000000
VERSION     DAOSTH010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50978_length_2023_cov_10.387703"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1264
                     /locus_tag="PLB65_08430"
     CDS             101..1264
                     /locus_tag="PLB65_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78593.1"
                     /translation="MSQDILSNFNADSREVTDAKIQAMRDAKLDTTQIEKQVAVLRQQ
                     DAAYKAQLKADSLAQQSAGKETYGYAHSTPGNMVSGASVEYPGGSKKMAGASFLDNLL
                     GTANAAENEGNYVQYDWEGEYRESEKKVGYFQSLVQNSIAEASKKMNDNYSKVETEKS
                     FTEEPNITDYSDYKITKFGLSAGFSAIKQVVMGSGIVEFGKDTWSQSSGLQKALYMGI
                     GLPVGLAIMNEAVEVKCDYKKSEDESLPISVKGQSVEIGNSSEFGKYFKANLNIEVTK
                     NSVKPTKDDYSDGFVINLGVDTSNNEVKVSGGVKKSVVINDNVSASGGFNYLQNAKGT
                     NYSLVMTGGLNGKLYESNWNVGFTKEISNMNVRREKNRNYLFFGVKVPISLGN"
     gene            1275..2006
                     /locus_tag="PLB65_08435"
     CDS             1275..2006
                     /locus_tag="PLB65_08435"
                     /inference="COORDINATES: protein motif:HMM:NF014543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78594.1"
                     /translation="MKNIFAIGLMLSQLLMCSHGLIGKDNSSNKDRIGKEEYVGIFSG
                     KYQGEELNGKAHGKGKVTSVQGDVLEGIFVNDEANGFFLIKYVDGQRFEGEFRKGVAE
                     GKGKFFWPEGDRYEGEYKNGKKNGYGIFYYAEGDKYEGYFKDGLRHGKGKYFAANGGS
                     YDGEYYKDKMHGKGIKIYRNNCKYIGDFKDGKLSGFGTYYNPDGSYYQGEFANDKPHG
                     RGKLFDKNGKLLGEGVWENGVYKGK"
BASE COUNT          766 a          239 c          510 g          508 t
ORIGIN      
        1 aatgaaagag agaaaaagaa attaagggga ctggggatat ccccagcggc ttttctttgc
       61 gagctttctt tttgccgcga aaaaagaaag cgaaatcgat atgagtcagg acatattatc
      121 caacttcaac gctgattcca gagaagtgac agatgccaag atacaggcga tgagagacgc
      181 taaacttgac acgacacaga tagaaaaaca ggtagcggta ctcagacagc aggacgcagc
      241 gtataaggcg cagttaaaag cggatagtct tgcacagcaa agcgcgggaa aagaaacata
      301 cggatatgcg cattcaacac cgggaaatat ggtatcagga gcttcggtag aatatcctgg
      361 cggaagtaaa aaaatggcgg gcgctagctt cttagataat cttttgggga ctgcaaacgc
      421 cgctgaaaac gaaggaaatt atgtacagta cgattgggaa ggagaataca gggagtctga
      481 aaaaaaagtt ggatactttc agagtcttgt tcagaattcc atagctgaag cgtcaaaaaa
      541 aatgaatgat aattatagca aagttgaaac ggaaaaaagt tttacagaag aacctaatat
      601 aacagattac agtgattaca aaatcactaa gttcggattg tctgctggat tttctgcgat
      661 taaacaagta gtaatgggat cgggcattgt agaattcgga aaagatacat ggagtcagag
      721 ttcaggattg cagaaggctc tttatatggg aatcggtttg ccggtaggat tggcaataat
      781 gaacgaggcg gttgaagtaa aatgtgatta taagaaatca gaagatgaga gtttgcctat
      841 tagtgtaaaa ggacaaagtg tagaaatcgg caatagcagt gaatttggta aatattttaa
      901 ggcaaatttg aatatagagg ttactaagaa tagtgttaaa cctactaaag atgattattc
      961 tgatggattt gtgataaatc tcggagttga tacatctaat aatgaggtta aagtttcagg
     1021 cggggttaag aagagtgtag ttataaatga taatgtttca gcctcaggag gatttaacta
     1081 tcttcaaaat gccaaaggaa caaactatag tttagtgatg acaggggggt taaatggaaa
     1141 actatatgaa agcaattgga atgtaggttt tacaaaagaa atatcaaata tgaatgtaag
     1201 aagagagaag aatagaaatt atttattctt tggagtaaaa gtcccgatca gtttagggaa
     1261 ttaggagaaa ataaatgaag aatatattcg caataggatt gatgctgtca cagctattaa
     1321 tgtgcagtca tggattgatt ggcaaagata attcaagtaa caaggatcga attgggaaag
     1381 aagaatatgt gggcatattc tcggggaaat atcaaggaga agaattaaac ggaaaggctc
     1441 acggaaaagg caaagtgact tcagttcaag gggatgtatt agaaggaatt tttgtaaacg
     1501 atgaagcaaa tggattcttt ttaattaagt atgttgatgg acaaagattt gaaggagaat
     1561 tcaggaaagg ggttgcggaa ggcaaaggaa agttcttttg gcctgaagga gacagatatg
     1621 aaggagaata taaaaacggt aaaaaaaacg gatatggaat attttattat gctgaaggag
     1681 ataagtatga gggatatttt aaagacggat taaggcatgg taaaggcaag tattttgctg
     1741 caaatggagg atcatatgat ggagaatatt ataaagacaa gatgcatggt aaaggaatta
     1801 aaatatacag aaataattgc aagtatatag gtgattttaa agatggtaaa ctaagcggtt
     1861 tcggcactta ttataatccg gacggttcgt attatcaagg agagtttgct aatgataaac
     1921 ctcatggacg cggaaaatta tttgataaaa atggaaaact gttaggagaa ggcgtttggg
     1981 aaaacggcgt atataaaggc aaataaacac aaggaagcaa tga
//
LOCUS       DAOSTH010000151         1938 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_54275_length_1938_cov_11.710037, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000151 DAOSTH010000000
VERSION     DAOSTH010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54275_length_1938_cov_11.710037"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="PLB65_08440"
     CDS             complement(<1..1275)
                     /locus_tag="PLB65_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78595.1"
                     /translation="MIGVGATRIEDRVFAALGIRKAKIFFEPCESVEYGGVLLLLPFL
                     IANGLLSYKNYYSERLSGYYNFDNVMLSLAFMYLCRIKSIEQLKHFSPGELGKLLGLD
                     RIPEARCMRGIIKEITVQERASQWNAYLSEQWINQEDTCIYYIDGHVQVYHGHLANLG
                     KKHVSRQKLCLPGMVEFWVNNADGLPYFYVTGQVNEKLQEMLTTELVPRLLELTDGRV
                     SQEDLDADHRLPRFTLVFDREAYSPVFFKHLWENFRIAVITYRKNIKDQWEEEEFNVH
                     DIHTDVKSTMVLCEKQVELNGVALREVRRLTDTGHQTSVITTNYKMSTALIALYMFSR
                     WAQENFFRYMRQEYDIDRIIQYGVNELDKSIMVVNREYSNLTYRLKKLRERIARRQAK
                     LYVLLEKNISDEMEQTGKNIQKQLLLSKEIELF"
     gene            complement(1344..1793)
                     /locus_tag="PLB65_08445"
     CDS             complement(1344..1793)
                     /locus_tag="PLB65_08445"
                     /inference="COORDINATES: protein motif:HMM:NF019096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUMOD1 domain-containing DNA-binding protein"
                     /protein_id="HOQ78596.1"
                     /translation="MQQILPIFPQDIKMVNYQVGFKSMDDFVHYFVNGMPVYCHELND
                     KNGYRYVLATLVNHNFCSIKELSNALGVNRKNIERYAKDLREKGMSHFFNRKETRGQC
                     YKFTPEKMNQAQRLLDQGYSQYRTAKIVGVSESAIRYHIKANSLKKK"
BASE COUNT          569 a          412 c          325 g          632 t
ORIGIN      
        1 aaataattct atttctttgc tcaggagtaa ttgcttctga atattctttc cggtttgttc
       61 catttcatct gaaatatttt tttccaataa cacataaagc tttgcttgac gccgagctat
      121 tctctctctc aattttttaa gtctatatgt aagattacta tattccctat taactaccat
      181 aatggattta tctaactcat taacaccgta ttgtattatt ctatctatat cgtattcttg
      241 cctcatatat ctaaaaaagt tttcctgcgc ccaacgtgaa aacatgtata aggcaataag
      301 agctgttgac atcttataat tggtcgtaat cacactggtt tgatgaccgg tatcagtaag
      361 cctgcgtact tcacgtaatg caacgccatt gagttctacc tgtttttcac ataagaccat
      421 ggtggatttt acatcggtat gaatatcatg tacattaaat tcttcttctt cccattgatc
      481 ttttatattc tttctgtagg taatcactgc gatgcggaag ttttcccata agtgtttaaa
      541 aaataccgga ctgtatgctt ccctgtcaaa tacaagggtg aacctgggaa gcctatggtc
      601 tgcatcaaga tcttcttggc tgactctgcc atcagttagt tcaagcagcc ggggaaccaa
      661 ttcggtggta agcatttcct gaagcttttc attcacctgc ccagtcacat aaaaataagg
      721 caacccatcg gcattattca cccagaactc aaccattccc ggcagacaaa gcttctgacg
      781 gctaacgtgt ttcttgccca gattggctag atgtccatga tatacctgta catgaccgtc
      841 gatatagtat atgcacgtat cttcttggtt tatccattgc tctgaaaggt aagcattcca
      901 ctgcgaagct ctttcctgaa cggttatttc tttaatgatc cctctcatac aacgagcctc
      961 tggaattctg tccagaccca gaagctttcc tagttcacca ggactgaagt gtttgagttg
     1021 ttcaatgctt ttgatacggc acaggtacat aaaggcaagg cttagcataa cattgtcaaa
     1081 attatagtac cccgaaagcc tctctgaata ataattctta tagctcaaca atccattggc
     1141 aataaggaaa ggaagcaata ataagactcc tccatattca acactttcac atggttcaaa
     1201 aaaaatcttt gccttacgta tgccaagggc agcaaataca cgatcctcaa tacgagtagc
     1261 tcctacacct atcatttctg aagccagaaa atcagcaata tctcgttcac agggtgtagt
     1321 agccgatact ggactgtaag tagttacttt ttttttaaac tatttgcctt aatatgataa
     1381 cggattgcac tctcacttac acctacaatt tttgctgttc gatattggga atatccctga
     1441 tctaaaagac gttgtgcctg attcatcttt tccggagtaa acttgtagca ttgaccccga
     1501 gtttcttttc gattaaaaaa gtgagacata cccttttcac gcaaatcctt agcataacgc
     1561 tctatatttt tcctgtttac acctaaagca ttgctcaact ctttgatact gcaaaaatta
     1621 tgattaacaa gagtagctaa tacataacga taaccgttct tgtcatttaa ctcatggcaa
     1681 taaacaggca ttccatttac aaaataatgg acaaagtcat ccattgattt aaatcctacc
     1741 tgataattaa ccatctttat gtcttggggg aaaataggaa gtatctgttg catatctgtg
     1801 tttttggcaa atttacgaca tttattgtct gcgataggtg aaaggtaacc tactgattct
     1861 caaagatggg atcaggagtt ctgaatttgg taattgaaca ctacaccaaa ctgtggaata
     1921 ttgaaaaggc atttcgta
//
LOCUS       DAOSTH010000152         1916 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_55148_length_1916_cov_13.423966, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000152 DAOSTH010000000
VERSION     DAOSTH010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55148_length_1916_cov_13.423966"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1364
                     /locus_tag="PLB65_08450"
     CDS             <1..1364
                     /locus_tag="PLB65_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOQ78597.1"
                     /translation="NRLYESGNFVFAYPNIICKAELFSTFPNDPYFQFQITCHNTGQT
                     FNGHGGTSDADIDAPEAWDITTGNNNIIIAVFDEGVTSDHPDLPNTRQVRLNGSNYGS
                     GDANNPSPTGDDNHGNSCAGVIGATMNNNEGIAGIAPNCRIMPLRWDATTTSDEMADG
                     IEFAVDNGANIISNSWGYSTSNNNYIPAIVTAIQYAINNNVVVVFAAGNTARHNSCCD
                     NGYVTFPANANVNGLLTVGASDRFDNQADYSPTSSLIDIVAPSHRAYPPEAYSPSCGG
                     ISGETFEMWTLDIPGDAGYNTWPSTGIHPPATGETLPNTGTNNLSYTGRFGGTSHACP
                     VVAGVAALLLSENPNLTPQEVFNTLTNTADKIGGYTYTDGRCNQTGFGRVNAFNALQA
                     LCTTTNFVNQTVTTNTTVEDCNVNVQNVDVTNNSKLTIEAENEVIINGEFEVELGSEL
                     EIK"
     gene            1515..>1916
                     /locus_tag="PLB65_08455"
     CDS             1515..>1916
                     /locus_tag="PLB65_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78598.1"
                     /translation="MKQSANLKHIPTMKIAFQIQLIVSAVFFFGLGCTSDDPKPASKD
                     KITGTWIEKNPELFDGVSDTIVFTEDALIRKHFYFAGWSYSTSSDTISFQKEETIKKF
                     TYSTEKENEMVIYNFIDRLITSQVKDIPFIKI"
BASE COUNT          632 a          355 c          378 g          551 t
ORIGIN      
        1 caaataggtt gtatgaatcc gggaattttg tttttgcata tccaaatatt atttgtaaag
       61 ccgaattgtt tagcacattt ccaaatgacc cgtattttca gtttcaaatt acttgtcaca
      121 acacaggtca aacatttaat gggcatggcg gaacatctga tgctgatatt gatgcacctg
      181 aagcatggga tattacaacg gggaataaca atattataat tgcagttttt gacgaaggag
      241 ttacttcaga ccatcccgat ttacctaata caagacaagt aaggctaaat ggtagtaatt
      301 atggttcagg tgatgcaaat aatccatcac caacaggaga cgataatcat ggaaattcct
      361 gtgctggtgt tatcggggct actatgaata ataatgaagg aattgcagga attgcgccaa
      421 attgtagaat tatgccttta agatgggatg ctactacaac ttctgatgaa atggcagacg
      481 gcattgagtt tgctgtggat aatggagcaa acatcatttc taatagttgg ggttatagta
      541 catctaataa taactatata cctgcaattg taacggctat tcaatacgcc ataaacaata
      601 atgtggtagt tgtttttgct gctggtaata cagctcgtca taattcttgc tgtgacaacg
      661 gttatgttac attccctgca aatgcaaatg taaatggtct tctgacagtt ggtgcttcgg
      721 atagatttga caatcaagcc gattatagtc ctacaagctc actaatagat atagttgccc
      781 cctctcacag agcatatccc ccagaggctt attcccctag ctgcggtggt attagcggcg
      841 aaacttttga aatgtggact ttagatattc caggagatgc tggatataat acatggccat
      901 caacaggtat tcatccgcct gcaactggtg aaacattgcc caatacagga acaaataatc
      961 tttcatatac aggtcgtttt ggaggaactt ctcatgcttg ccccgttgtt gccggtgtag
     1021 ctgcattatt attatcagaa aaccccaact taacgcccca agaagttttt aacactttaa
     1081 caaatacagc agacaagata ggaggatata catataccga tggtagatgc aatcagacag
     1141 gttttggtag agtaaatgct tttaatgcat tacaagcttt atgtactacc accaattttg
     1201 ttaatcaaac tgtaactaca aatactacgg ttgaagattg taatgtaaat gttcaaaatg
     1261 ttgatgtaac aaataactca aaattaacaa ttgaggctga gaatgaggtg ataataaatg
     1321 gagaatttga agttgaatta ggctctgaat tggagataaa ataagtactg gctctaatgc
     1381 gtcatacgta atggcggggc aagtagtata tatcaaggtt tgcggctcgc atcaacacca
     1441 tctcggatag agaaaagctc cgaagtccgc cactacgcat acacccccgt aaacatcgtt
     1501 catagccaaa agcaatgaaa caaagtgcta atcttaaaca tataccaaca atgaaaatcg
     1561 cgtttcaaat ccaactgatt gtaagtgcag tattcttttt tggtttggga tgtacaagcg
     1621 atgatcctaa gcctgcatca aaagacaaaa ttaccggaac gtggatagaa aagaatcccg
     1681 aattattcga cggcgtctcc gacaccatcg tcttcacgga agatgcgctg ataaggaaac
     1741 atttttattt cgctgggtgg agttattcta cctcttccga taccatttca ttccaaaaag
     1801 aagaaactat aaagaaattt acgtattcaa ccgaaaaaga gaacgaaatg gttatttata
     1861 acttcatcga caggctgata acttcccagg taaaagacat tccatttatt aaaatt
//
LOCUS       DAOSTH010000153         1873 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_56973_length_1873_cov_13.349285, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000153 DAOSTH010000000
VERSION     DAOSTH010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56973_length_1873_cov_13.349285"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(23..178)
                     /locus_tag="PLB65_08460"
     CDS             complement(23..178)
                     /locus_tag="PLB65_08460"
                     /inference="COORDINATES: protein motif:HMM:NF019493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HOQ78599.1"
                     /translation="MPINPDTHASVSVNMPKELYEQLKVLAKKNHRSVSAEICRLVEK
                     ELEAAKE"
     gene            319..462
                     /locus_tag="PLB65_08465"
     CDS             319..462
                     /locus_tag="PLB65_08465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78600.1"
                     /translation="MVKTFPIVMDDKFHERLQKAAEKEDKPIKALILEAIEQKIIEVE
                     EGE"
     gene            463..1200
                     /locus_tag="PLB65_08470"
     CDS             463..1200
                     /locus_tag="PLB65_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78601.1"
                     /translation="MPNRIIKESICTSDNLDKLSPEAEVFFYRLMVTCDDYGIMFAAP
                     SILRSKCFPWRIDKIKDKDIEKWRTELIEAELIFLYEYDGKTYLKFSKWEQHQQVRAN
                     KSKYPTPDMEGVKLISNDINGNQKISNVPVFDISNRNSNRDKIIGEYTTNSELKQTIF
                     DFIEMRNKIKKPMTDKAISLLLKKLSELATADEEKILILEQSIANSWQGVFELKGSNS
                     NGSNKQHNGEDKKTFKLPPEFYAVRST"
     gene            1118..1864
                     /locus_tag="PLB65_08475"
     CDS             1118..1864
                     /locus_tag="PLB65_08475"
                     /inference="COORDINATES: protein motif:HMM:NF005378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOQ78602.1"
                     /translation="MEAISNITERIKRLSNSHPNFTQSAAHDCEICKDTEWIINTETN
                     SATPCKCQEAKHYKRILENCGISEVFLKKTFHNYIPKNQTQREAKDMAQEYAKNFQQI
                     RNERNNSIAFLKQVGSGKTHLSIAISNKLMEQGVGVRYMQYREVITHLKQNIVDEEFY
                     QREMSKYKNAPVLLMDDLYKGKTTESDINLVYEIINYRYLKGMPIILSCEYSVDKLLD
                     FDEAIGSRIAEMCKGRIVEFIGTELNDRLR"
BASE COUNT          753 a          294 c          365 g          461 t
ORIGIN      
        1 gaaagttgga agtaaaagct gcctattcct tggcagcttc caactccttc tcaaccaatc
       61 ggcatatttc agcagatacg cttctgtgat tcttctttgc cagtaccttc aactgctcat
      121 acaactcttt aggcatattc acagaaacgc ttgcatgagt gtctggatta atcggcataa
      181 tatcccctcc caataactat tatacacagt ccacagaaaa aaataaacaa taaataatat
      241 gtcgattgcg gtaaataaca ataaataaat gttgtaataa gttagtaact atgttaaaat
      301 gagagagagg tgagaaatat ggtgaaaaca tttcctatag tgatggatga taaattccat
      361 gagagattgc agaaagcggc tgagaaagag gacaagccta taaaggctct tatccttgaa
      421 gcgatagaac agaagataat tgaggttgaa gaaggtgagt aaatgccaaa ccgtattata
      481 aaagaatcaa tatgcaccag tgataatctg gataaattaa gcccagaagc agaagttttt
      541 ttctataggt taatggttac ttgcgatgat tatggaataa tgtttgcagc accttctata
      601 ttacggtcaa agtgttttcc gtggaggata gacaaaatca aggataagga tatagagaaa
      661 tggagaacag aacttataga agcagaattg atatttttgt atgaatatga cggcaaaaca
      721 tatttaaaat tttcaaaatg ggagcaacat cagcaagtga gagcaaataa aagcaaatat
      781 cccacacctg atatggaagg ggttaaattg atatcaaatg atatcaatgg aaatcaaaag
      841 atatcaaatg tccccgtatt cgatataagt aatcgtaata gtaatcgaga taagataatc
      901 ggagagtata cgactaattc agaattaaaa cagacaattt ttgattttat agaaatgcgt
      961 aataagataa aaaaaccaat gacagataaa gcaatatcac tattactaaa gaagctttca
     1021 gaattagcaa cagccgacga agaaaaaata ttaatactag aacaatcaat agcaaatagt
     1081 tggcaggggg tatttgagtt gaaaggaagt aatagcaatg gaagcaataa gcaacataac
     1141 ggagaggata aaaagacttt caaactccca cccgaatttt acgcagtccg cagcacatga
     1201 ttgcgaaatt tgcaaagata ccgaatggat cataaatacc gagacaaact cagcaacgcc
     1261 ctgtaaatgt caagaagcca aacattataa acgaatcctt gaaaattgcg gaatatcaga
     1321 agtatttttg aaaaagacat tccacaacta cataccaaaa aaccaaacac aaagagaagc
     1381 aaaagacatg gcacaagaat atgcaaagaa ttttcaacag atacgaaatg aacggaataa
     1441 ctcaatagcg tttctaaaac aggtcggctc cggcaagaca catttatcta tagcaataag
     1501 taataaactc atggaacaag gtgtaggagt gcggtatatg cagtatagag aagtcataac
     1561 acacctaaaa caaaacatag tagacgaaga attttaccaa agggaaatga gcaaatacaa
     1621 aaatgcaccg gttctactga tggacgattt atataagggc aagaccacag aatcagacat
     1681 caatcttgtc tatgaaataa tcaattacag atacttgaaa ggtatgccaa ttatattaag
     1741 ttgcgaatac agtgtagaca agctattaga ttttgatgaa gcaatcggca gtaggatagc
     1801 ggaaatgtgc aagggcagaa ttgttgaatt tatcggtacc gaattaaacg ataggttgag
     1861 gtgatgaaat gaa
//
LOCUS       DAOSTH010000154         1714 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_64928_length_1714_cov_10.346594, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000154 DAOSTH010000000
VERSION     DAOSTH010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64928_length_1714_cov_10.346594"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            205..>1714
                     /locus_tag="PLB65_08480"
     CDS             205..>1714
                     /locus_tag="PLB65_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HOQ78603.1"
                     /translation="MPNPILVTKPFLPNIQDFYSKLLGLWQSRVLTNSGELHQLLEKQ
                     LTEKLDVTSISLINNGTIALEVAIQALDLKGKIITTPYTFIATANSILLKGLDPVFID
                     IEDNGFNLDPELIDEVKDKDVSAIMPVHVYGLPCDDESIGQRAKGRGLKVIYDAAHAF
                     GIKKKGESILNWGDASTLSFHATKVFHTFEGGAVVCNDPLLKNKINQVRNFGYTSDSF
                     DIITLGTNGKMNEAQAAMGLALIPYLEEIITKRKHWFNFYVEQLSEIPGIKLMTIPSD
                     LEYNYSYFPILVQSGFSLTRDELFNRFQEENIFPRKYFYPLITDFSLYKNYNIISKHK
                     IPNARKIADSVICLPLYPDLTEDDVSRVCEVIKKAEAEGGEQRAEGGEMMDKEMYRFE
                     KLDVWKKGLELSDRLFDIADRTDMKHCYRFSEQLRAAAMSITNNIAEGSASTSDKDFA
                     QFLNIARRSVFECVNILILFERRKYITLDELDSLKLELSSISKMLTNLRKSLL"
BASE COUNT          559 a          277 c          314 g          564 t
ORIGIN      
        1 tttgttgctt tagatattca ttacatagat gtaaagcaac acaatgaact aatgttaaaa
       61 ctaaatagta tcaaaagttt aattggcggt tttatccgct accttaaaaa ctctgaacac
      121 actaactaag aactttgaac tttgaactcc cgcactttga actcctgcac tttgaacttt
      181 gaactttgaa ctcccgcact aaaaatgcct aacccaattt tagtaactaa acccttctta
      241 cccaatatcc aagattttta ttctaagttg cttgggctct ggcaatccag agttcttacc
      301 aatagtggtg aattgcatca gctattagag aagcaattaa cagaaaaatt agatgtcaca
      361 agtatttcct tgattaataa tggcactata gcattggaag tggcaattca agcattagat
      421 ctaaaaggga aaattattac taccccctat acttttattg ctaccgctaa ttctattttg
      481 cttaaaggac ttgatccggt ctttattgat attgaagata atggcttcaa tcttgatccg
      541 gaattgatag atgaagttaa ggataaagat gtatcagcaa ttatgccggt tcatgtttat
      601 ggcttaccct gtgatgatga aagcataggg cagagggcga agggcagagg actgaaggtt
      661 atttatgatg cagctcatgc ttttggaatc aagaaaaagg gagaatcaat tcttaattgg
      721 ggtgatgcct ctacattaag tttccatgct actaaggttt ttcacacttt tgaagggggc
      781 gcagttgttt gcaatgatcc tttattaaaa aataaaatca accaagtaag aaattttggt
      841 tatacatctg atagttttga tattattact ttgggaacaa atggaaaaat gaatgaagcc
      901 caagctgcta tgggcttagc tcttattcct tatcttgagg agataataac taaaagaaag
      961 cattggttta atttttatgt agaacagctt agtgaaattc cgggaataaa gttaatgact
     1021 attccttccg atcttgaata taattattca tattttccta ttctggttca gtcaggtttt
     1081 tctttaaccc gagatgaatt attcaatcgc ttccaagaag aaaacatttt tccccgtaaa
     1141 tacttttatc ccttaattac ggacttttct ctttacaaga attataacat tatctctaaa
     1201 cataaaatcc ctaatgctcg taaaattgcg gattcagtaa tttgccttcc actttaccct
     1261 gaccttacag aagatgatgt atccagagta tgcgaggtca tcaaaaaagc tgaggcagag
     1321 ggcggagagc agagggctga gggcggagag atgatggata aagaaatgta tagatttgaa
     1381 aaactggatg tgtggaagaa aggtcttgaa ttgtctgata gattgtttga tatagcagat
     1441 agaactgata tgaaacattg ttatagattt tcagaacagc ttagagctgc agctatgagt
     1501 attactaata atattgcaga aggttctgct tcaacttctg ataaagattt tgctcagttt
     1561 ttaaatattg ctcgtcgttc tgtctttgaa tgtgttaata ttttaattct ttttgaaaga
     1621 agaaagtata ttacattgga tgaattggac tctttaaaat tagaactatc ctccatcagt
     1681 aaaatgctaa ctaacttacg caaatctcta ttaa
//
LOCUS       DAOSTH010000155         1703 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_65516_length_1703_cov_8.490291, whole genome shotgun sequence.
ACCESSION   DAOSTH010000155 DAOSTH010000000
VERSION     DAOSTH010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65516_length_1703_cov_8.490291"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(404..1363)
                     /locus_tag="PLB65_08485"
     CDS             complement(404..1363)
                     /locus_tag="PLB65_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOQ78604.1"
                     /translation="MTLEYLWIEKYKCFEKQGVNLGGEYLFRIDLSHYLTGTITIISK
                     NNPSYIHDFYNLPAANQVANVANITAIVGENGSGKTTLFDFIKLYFPDGEGEINTCCL
                     FITRENRGPYTVHSYNERDIKILDETGLNLKIEKRKFDTSSRVTAVGTKPFKDVNIIY
                     FSNIFDLRSEYEVAGLNNLSTNYLVRNEKNRITEYKAHQAQFITEVQAYQNSEIDKQL
                     RFVRYYYNKNKLLALPFKLPDKLIIERANGRTIKVIDILRSKQIYNKVYNKSMGCLVQ
                     NLEHLSDKEIRRALFSVSSIPEEYHERPLSKFINDILDQYQST"
BASE COUNT          490 a          335 c          284 g          594 t
ORIGIN      
        1 ggaataggat gaattcatct caattcaaat gcgggtgtat tattcaacgg tcttcaattt
       61 atatgcaatg gttctttcaa tagtctcctt gcacgcaggc attaactacc agacactcgc
      121 tcaagtatac aatgcaacgt tgctttcact tcttatcaat ccagttccga aacaggatgc
      181 tatgtatgat ttggctatct aacaatagtt tcaaaagcaa gctacaattt aggacttcac
      241 gatgctatct ttagcgagta agtgtttttt ttgttgaagg tgtgcgtcta atctgccatc
      301 caggcatatc ttaggaaaag agagatagat acagattatc ctttaattat tcataaagct
      361 ccagaagtat tcgcctacta cctccaattg attgtttaca attctatgta ctctgatatt
      421 ggtccaatat gtcgttaata aattttgata gtggcctctc atgatattct tcgggtatcg
      481 aagaaacact aaaaagagct ctacgaattt ctttgtcgga taaatgttct aaattctgaa
      541 ctaaacaccc catgctctta ttgtaaactt tattatatat ttgtttggac cttaagatat
      601 caattacctt tattgttcga ccatttgctc tctcaataat tagcttatca gggagtttaa
      661 atggtaaggc taacagttta tttttattgt aataatatct aacaaaacgt agttgtttat
      721 ctatttcaga gttttgatat gcttggacct cagttatgaa ttgtgcttga tgggctttat
      781 actccgttat cctgtttttc tcgtttctaa ctaaatagtt agtagataaa ttattaagac
      841 ctgctacctc atattcactt cttaggtcaa agatatttga aaaatatata atattaacat
      901 ctttaaaagg ctttgttcca actgctgtaa cacgggagct tgtatcgaac ttcctttttt
      961 cgattttcaa attcaatcca gtctcatcta aaattttaat atcacgctca ttgtaggaat
     1021 gtactgtgta aggacctcta ttttctctag taataaatag acagcaggtg ttaatctctc
     1081 cctctccatc gggaaaatag agtttgataa agtcgaacaa ggttgtcttg cctgatccgt
     1141 tttctccgac aattgcggtt atgttagcaa cattggctac ttgatttgcg gcgggcagat
     1201 tgtaaaaatc atgaatatat gacgggttat tcttggatat tatagtaatt gtaccagtaa
     1261 ggtaatgtga taaatcaatt ctgaaaagat actcacctcc aaggttaaca ccctgtttct
     1321 caaagcattt gtatttctct atccaaagat attctaatgt cataaaaact cctaagctat
     1381 acaagctgat tcatggagtc ccaatgttgt tgtacaatcc aacccattgt ttctctgaat
     1441 attagaaggc atctgatatc cccgttactc actatctatg caaggccaaa cgaccattcg
     1501 cattgttttc atcttgcaaa tccaagtcaa gttctttttt caagtgagct tatccggctg
     1561 ttcatttcct gacccgccct ttgccccagc ccatttgcct ccttttcgcc tctcgctgag
     1621 ttcccgtttg acgggcggga actaagcgag aaaagaccgt tgaataatac acccgcattt
     1681 gaattgagat gaattcatcc tat
//
LOCUS       DAOSTH010000156         1694 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_66039_length_1694_cov_13.001830, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000156 DAOSTH010000000
VERSION     DAOSTH010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66039_length_1694_cov_13.001830"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(216..1442)
                     /locus_tag="PLB65_08490"
     CDS             complement(216..1442)
                     /locus_tag="PLB65_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HOQ78605.1"
                     /translation="MRKFILPLLIAFIYLPFMFADNIYVVNSESRTLSRIDTDTQTVN
                     NTFTQLGLTPNLLKVNEDYIYVVLSGANSIQMIERESGTTLRNIFIASSSNPWDVVEY
                     DGYLYVTGMFSNKVYKISQESWSVVDQITVGTAPEGLAVCNGKLYVCNTGGYASNYAN
                     SSVSVIDLVDFSVIKTIPVWTNPQDIAVLDSQLHIVCTGNWSSISGAVDVIDSITDEK
                     TDRIMIGGNPWGIWISPDGIAYLGEGYNSGIYSYNANTLSVLNNSSNPLSPGGIAVSG
                     LNEHIAVLSANWGENGIVYLRNSNWQPITQWTVALTPTDICYFESPSPVVDENLPQPK
                     ITLYPNPVKKEGVLNFKTDNNFRGELEIYNLKGQKVLQAKVTKGETSIDLKGKGLQSG
                     IYFYKSGQAQGKIIIK"
BASE COUNT          533 a          342 c          270 g          549 t
ORIGIN      
        1 taaaatcctt aaatcctcgt ttctaaaact aaatctgtgt aatctgtgta atctgtgaga
       61 ggatattttt ttcggtgtct tcggtggcta aatataacaa atcctgtaaa tccctaaaat
      121 cctgttcatc ccatacctat taaatctgcg taatctgcgt aatctgcgtg aaacaaaatc
      181 tgcgcaatct gcgggcaata ttttttctct gcagtctatt tgattattat tttcccctgt
      241 gcctgtccgc ttttgtaaaa ataaattccg ctctgcaaac ccttcccttt aaggtctata
      301 cttgtttccc ctttggtaac tttagcttgc aaaactttct gtccctttag gttatatatt
      361 tccaattctc cccgaaagtt attatccgtt ttaaaattta ggacaccctc ttttttaacg
      421 ggatttgggt ataaggttat ttttggctgg ggtaaatttt catccacaac aggtgaaggg
      481 ctttcaaaat agcaaatatc ggtgggagtt aaggcaacgg tccactgagt gataggttgc
      541 caattactat ttcttagata cacgattcca ttttcacccc aattagctga aagaactgca
      601 atatgttcat ttaagccact aactgctatt ccacctggac ttaaaggatt gctactatta
      661 tttaaaacag ataaagtatt ggcattataa gaatatattc cgctattata accctctccc
      721 aaataagcaa ttccatcggg actaatccat ataccccaag gattgcctcc aatcattatc
      781 cggtctgttt tttcatcggt tatggaatca attacatcaa ccgctccgga aattgaactc
      841 caattgccag tgcaaacaat atgcaattgg gaatcaagaa cggcaatatc ctgaggattg
      901 gtccaaacag gaatggtctt tataacgcta aaatcaacta aatcaataac agaaacactg
      961 ctattggcat aattactggc ataacctcct gtattgcaaa catatagttt tccattacat
     1021 acggctaaac cttcaggtgc cgtgccaacc gttatttgat ctacaacgct ccaactttct
     1081 tgactgattt tatagacctt gttagaaaac attccggtca cataaagata accatcatat
     1141 tctacaacat cccaaggatt ggaactggaa gcaataaaaa tgttgcgaag agtggttccg
     1201 gattctcttt caatcatctg aatactattc gctcccgata agaccacata aatataatct
     1261 tcattcactt tcagtaaatt gggcgttaaa cccaactgag taaaagtgtt gtttacagtt
     1321 tgggtatctg tatcaatcct gcttaaagtg cgggattcgg aatttactac atagatatta
     1381 tctgcaaaca taaaaggcag atagataaaa gctatcagta acggcagaat gaacttacgc
     1441 attgtaacct ctatatattt tatttatttt tagtaacata ttttgttatg cttcaaactt
     1501 tctaaaccca caattctatt ttagctcttt atgtcaaatc ccatttctat atattaaggg
     1561 ggtgacaaat ctcaattttg tcacccgccg caagaactga cggcaaaata gtgagatata
     1621 aattctgagg aaagcatagt tattggagag aacaaaaaga aaataattat ggcttgatta
     1681 caaaattata aatc
//
LOCUS       DAOSTH010000157         1691 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_66192_length_1691_cov_13.746333, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000157 DAOSTH010000000
VERSION     DAOSTH010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66192_length_1691_cov_13.746333"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..>1691
                     /locus_tag="PLB65_08495"
     CDS             176..>1691
                     /locus_tag="PLB65_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78606.1"
                     /translation="MKSPKLRSLPLGLILFFLCFSISPCLAQFGGGTGSVASPYLVAT
                     AQHLSNVRDYADAYFLQTGDIFLNVEPYNSGQGWLPIGNQSAPFTGSYDGNSFHIYGL
                     YINQPEGKNLGLFGYTNSAVLKNIALNNVNISGKDYIGSLVGSVNGSFIQSCSCDGTI
                     TGVSVTGGLVGALELSCHLEKCSADCVVSGQFSIGGLCGYSKGASEIYTSYSKGTVTG
                     NNATGGLVGMQGNSTISQSFSASAVQGNWGAGGLLGVNDTQSLVSNCYSIGVVTGNSN
                     TGGLIGRNVVSQTLSSYWNLESSGQVTSAGGIGRTTDEMTFTYAANTYEDWNLTEIWQ
                     PDTEYFVNAGYPYLQWMYPCPPVSHLSYLIENYSVILQWSPPYVSSYTGFNVYKNGEL
                     YTAVTDSFFIDTDVYHGEQYTYQVSVVYEGGESSLSEPVSFYVYFPPLPANCVFPLNG
                     STEMDILPLIQWSPDAHHNYPLPDGYLLEVWALSGTHVVDEDVGNVTSYQITNPL"
BASE COUNT          468 a          312 c          354 g          557 t
ORIGIN      
        1 aaaatgttta ttaaagttat tgccgttaac gaataagcgt gaacactatg aaagacaccc
       61 agatacactt aagctattct aaaaagacga aaaaccttaa ccggatatat tactgcaggc
      121 aaaaaagcga tgaactataa ttatagacaa taaaaataac ttcattggag gaacgatgaa
      181 atccccaaaa ctgagaagcc tacctttagg acttatttta tttttcctct gtttttccat
      241 cagcccttgt ttggctcaat ttggcggagg aacaggttct gtggcatcac cctatttagt
      301 agcaactgca caacatttat ccaatgtgcg tgattatgcc gatgcatact ttttgcaaac
      361 aggtgatatt tttctgaatg ttgaacccta taattcagga cagggctggt tacctattgg
      421 caatcaatcc gctcctttta ccggttccta tgatggaaac agttttcata tttacggctt
      481 atatattaat caaccggaag gaaaaaatct gggtttattt ggctatacta attctgctgt
      541 gctgaaaaat attgccctta ataatgtaaa catcagcgga aaggactata taggttctct
      601 tgtcggctct gtaaatggca gctttattca aagttgcagc tgtgatggaa caattacagg
      661 tgtttctgta accggtggtt tagttggtgc tctggaactt tcctgccatc tggaaaaatg
      721 ctctgccgat tgtgtggtca gcggtcaatt ttcaataggt ggactttgcg gctacagtaa
      781 aggcgcaagt gaaatttata cttcatacag taaaggaact gttaccggta ataatgctac
      841 aggtggtctg gttggtatgc agggaaattc tactataagc caaagcttct ctgcatctgc
      901 tgttcagggc aattggggtg cagggggatt acttggagtt aatgacaccc aaagtttggt
      961 ctctaattgt tattctatcg gagtagttac cggcaatagt aataccggtg gacttattgg
     1021 cagaaatgtt gtcagtcaga ccctttccag ttattggaat ttagaaagca gcggacaagt
     1081 aacttctgca ggtggaattg gtagaactac ggacgaaatg acttttacgt atgccgctaa
     1141 cacttatgag gattggaatt taaccgaaat ttggcaaccc gatactgaat attttgttaa
     1201 tgcgggctat ccttatctgc aatggatgta tccttgtcct cccgtttctc atttaagtta
     1261 tctaatagaa aattactccg ttattttaca gtggagccct ccttatgttt cttcttatac
     1321 gggatttaat gtctataaaa acggagagct ttatactgct gttaccgata gcttttttat
     1381 agatactgat gtctatcacg gtgaacaata tacttatcag gtgagcgttg tttatgaggg
     1441 tggtgaatct tcgctttctg agccggtttc tttttatgtg tattttcctc ctttaccggc
     1501 gaattgtgtt tttcctttaa atggctctac cgaaatggat atcttacctc ttatacaatg
     1561 gtctcctgat gctcaccata actatccttt gcccgatggc tatttattgg aagtttgggc
     1621 tttatcagga acccatgtgg tggatgaaga tgtgggaaat gttacttcct atcaaattac
     1681 taatccttta g
//
LOCUS       DAOSTH010000158         1635 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Candidatus Cloacimonas sp. isolate AMR_MDS_2220
            NODE_69651_length_1635_cov_13.577215, whole genome shotgun
            sequence.
ACCESSION   DAOSTH010000158 DAOSTH010000000
VERSION     DAOSTH010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32864287
            Sequence Read Archive: SRR4236674
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Candidatus Cloacimonas sp. (wastewater metagenome)
  ORGANISM  Candidatus Cloacimonas sp.
            Bacteria; Candidatus Cloacimonadota; Candidatus Cloacimonadia;
            Candidatus Cloacimonadales; Candidatus Cloacimonadaceae; Candidatus
            Cloacimonas.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 04:24:59
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,700
            CDSs (total)                      :: 1,680
            Genes (coding)                    :: 1,673
            CDSs (with protein)               :: 1,673
            Genes (RNA)                       :: 20
            tRNAs                             :: 17
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Candidatus Cloacimonas sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69651_length_1635_cov_13.577215"
                     /isolate="AMR_MDS_2220"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053534"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            177..>1635
                     /locus_tag="PLB65_08500"
     CDS             177..>1635
                     /locus_tag="PLB65_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOQ78607.1"
                     /translation="MKIRFCFIALISLVAILLCSCDSNTKIQKGVISGVIILDGQTDY
                     SGITVMLYSTDIVPEKLKEINKQYPQLAFPLKDKHIFDHRERTPLFTVVTNNNGFFSF
                     PEVVYGKYIITYLKEGWGYNYLFDIELNAQEISLNNTQKADLLLYPETIVPVTIEGNY
                     NFLQNHCYVVENDVTALPTSTLNFAQDAKILIAPSKKITIYGNCSFPEGDDFAYINSY
                     SGIYSSTNTEILNGEGIVYLGVTGTINNICIANQNTGISIRTNETTLHNLLVRDCVFG
                     ITSVQVNNLEISNSIIMDNKSQDAVAISNYTVNGCNVQDNIFYNNYISLKHEIVKNAV
                     VSNNAFISGSREFINSWESTCEFSHNLIDNVGIGIVNTGQSNLNISYNSIKANVCIQT
                     YHSHHSWNTVSNGWTKANNNNFYASQYVMESGASYIYPDGLPYPLDFKNNFWNTTSTT
                     EIDELIIDFNDLGLELNYGVTSIVNYVPYKNGPVAG"
BASE COUNT          565 a          237 c          292 g          541 t
ORIGIN      
        1 gccaaaccag ataatgctta acacgcatcc acaaagatgg aatgatgaat atatgaaatg
       61 ggggaaggaa ttggtggggc aaaatgtgaa gaaccaggta aaaagattgg tctctcacag
      121 attacacaga tgataactct cacagattac acagatttca cagattaagt actataatga
      181 aaataagatt ttgttttatt gccttaattt ctttagttgc tatattatta tgtagctgtg
      241 atagtaatac taagatacaa aagggtgtta tttccggggt tatcatttta gatggtcaaa
      301 cagattattc tggaattacg gttatgttat attctactga tatagttccg gaaaaattaa
      361 aagaaataaa taagcaatat cctcagcttg cttttccact taaggataag catatatttg
      421 atcatagaga aaggacacct ttgtttacgg ttgttactaa caataatggt ttttttagct
      481 ttccggaagt tgtatatggg aaatacataa taacatatct gaaagaaggt tggggatata
      541 actatctttt tgatatagaa ttaaatgccc aggaaatttc gttgaataac actcagaaag
      601 ccgatcttct cctatatccg gaaacaatag ttccagtaac aattgaggga aactacaatt
      661 ttttacaaaa ccattgttat gttgtagaaa atgatgtaac cgcgctacct acttcaactc
      721 ttaattttgc tcaagatgct aaaatcctta ttgctccaag taaaaagata actatctatg
      781 ggaattgttc ctttcccgaa ggggatgatt ttgcttatat aaattcatat tctggaattt
      841 attcttcaac aaacacagag attttgaacg gggaaggaat tgtttattta ggtgttacag
      901 gaacaatcaa taatatatgt attgctaatc aaaacacagg aataagcatt agaaccaatg
      961 aaacgacttt gcataattta ttggtgagag attgtgtttt tggaatcact tctgtgcaag
     1021 taaacaactt ggaaataagc aattctatta ttatggacaa taaatctcag gatgcggtag
     1081 ctattagtaa ttatactgtt aacggttgta atgttcaaga taatatcttc tataataact
     1141 atatttcctt gaaacacgag atagtaaaaa acgctgtcgt tagtaataat gcttttatca
     1201 gtggttcacg ggagtttatt aattcctggg aatcaacttg tgaattctct cacaacctga
     1261 tagataatgt gggtataggc attgtaaata caggccaatc caatcttaat atttcatata
     1321 attccattaa agcaaatgta tgtattcaaa cctaccattc tcatcattca tggaacacag
     1381 tgagtaatgg ttggactaaa gcaaataaca acaattttta tgcttcgcaa tatgttatgg
     1441 aatcgggggc atcttatata tatccggatg gtttacctta tccattggat tttaagaata
     1501 atttttggaa tactacatca actacagaaa ttgatgaatt gataattgat tttaatgatc
     1561 ttggcttaga gcttaattat ggtgtaactt ctattgtaaa ctatgtgcca tataaaaatg
     1621 gtcctgtagc gggag
//
